LOCUS       DAHEYX010000001        57585 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHEYX010000001 DAHEYX010000000
VERSION     DAHEYX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.001"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            58..1575
                     /locus_tag="MB219_000001"
     CDS             58..1575
                     /locus_tag="MB219_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCC8425747.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1547..2836
                     /locus_tag="MB219_000002"
     CDS             1547..2836
                     /locus_tag="MB219_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8425748.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3011..3907
                     /locus_tag="MB219_000003"
     CDS             3011..3907
                     /locus_tag="MB219_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8425749.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3921..4784
                     /locus_tag="MB219_000004"
     CDS             3921..4784
                     /locus_tag="MB219_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8425750.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4790..5353
                     /locus_tag="MB219_000005"
     CDS             4790..5353
                     /locus_tag="MB219_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCC8425751.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5375..6886
                     /locus_tag="MB219_000006"
     CDS             5375..6886
                     /locus_tag="MB219_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCC8425752.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6898..7857
                     /locus_tag="MB219_000007"
     CDS             6898..7857
                     /locus_tag="MB219_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCC8425753.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7943..9022
                     /locus_tag="MB219_000008"
     CDS             7943..9022
                     /locus_tag="MB219_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8425754.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9274..10674)
                     /locus_tag="MB219_000009"
     CDS             complement(9274..10674)
                     /locus_tag="MB219_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCC8425755.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10892..12553
                     /locus_tag="MB219_000010"
     CDS             10892..12553
                     /locus_tag="MB219_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCC8425756.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12510..13208
                     /locus_tag="MB219_000011"
     CDS             12510..13208
                     /locus_tag="MB219_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCC8425757.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13224..14648
                     /gene="araA"
                     /locus_tag="MB219_000012"
     CDS             13224..14648
                     /gene="araA"
                     /locus_tag="MB219_000012"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCC8425758.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14955..16114
                     /locus_tag="MB219_000013"
                     /pseudo
     CDS             14955..16114
                     /locus_tag="MB219_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            16232..18091
                     /locus_tag="MB219_000014"
     CDS             16232..18091
                     /locus_tag="MB219_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCC8425759.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18201..18635
                     /locus_tag="MB219_000015"
     CDS             18201..18635
                     /locus_tag="MB219_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425760.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18894..19055)
                     /locus_tag="MB219_000016"
     CDS             complement(18894..19055)
                     /locus_tag="MB219_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425761.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19074..20096)
                     /locus_tag="MB219_000017"
     CDS             complement(19074..20096)
                     /locus_tag="MB219_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCC8425762.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20224..20577
                     /locus_tag="MB219_000018"
     CDS             20224..20577
                     /locus_tag="MB219_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCC8425763.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20589..21755
                     /locus_tag="MB219_000019"
     CDS             20589..21755
                     /locus_tag="MB219_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCC8425764.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21847..22467)
                     /locus_tag="MB219_000020"
     CDS             complement(21847..22467)
                     /locus_tag="MB219_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCC8425765.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22659..22955)
                     /locus_tag="MB219_000021"
     CDS             complement(22659..22955)
                     /locus_tag="MB219_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCC8425766.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23159..23809
                     /locus_tag="MB219_000022"
     CDS             23159..23809
                     /locus_tag="MB219_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCC8425767.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23825..25552
                     /locus_tag="MB219_000023"
     CDS             23825..25552
                     /locus_tag="MB219_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8425768.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25549..27441
                     /locus_tag="MB219_000024"
     CDS             25549..27441
                     /locus_tag="MB219_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8425769.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28145..29014
                     /locus_tag="MB219_000025"
     CDS             28145..29014
                     /locus_tag="MB219_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCC8425770.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29223..30248
                     /locus_tag="MB219_000026"
     CDS             29223..30248
                     /locus_tag="MB219_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCC8425771.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30287..31237
                     /gene="manA"
                     /locus_tag="MB219_000027"
     CDS             30287..31237
                     /gene="manA"
                     /locus_tag="MB219_000027"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCC8425772.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31331..33034
                     /locus_tag="MB219_000028"
     CDS             31331..33034
                     /locus_tag="MB219_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8425773.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33036..35069
                     /locus_tag="MB219_000029"
     CDS             33036..35069
                     /locus_tag="MB219_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCC8425774.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35080..35520
                     /locus_tag="MB219_000030"
     CDS             35080..35520
                     /locus_tag="MB219_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8425775.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35720..36847)
                     /locus_tag="MB219_000031"
     CDS             complement(35720..36847)
                     /locus_tag="MB219_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8425776.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36850..37527)
                     /locus_tag="MB219_000032"
     CDS             complement(36850..37527)
                     /locus_tag="MB219_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8425777.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37791..39557
                     /locus_tag="MB219_000033"
     CDS             37791..39557
                     /locus_tag="MB219_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCC8425778.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39572..40009
                     /locus_tag="MB219_000034"
     CDS             39572..40009
                     /locus_tag="MB219_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8425779.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40022..41173
                     /locus_tag="MB219_000035"
     CDS             40022..41173
                     /locus_tag="MB219_000035"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCC8425780.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41235..41933)
                     /locus_tag="MB219_000036"
     CDS             complement(41235..41933)
                     /locus_tag="MB219_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCC8425781.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41926..43278)
                     /locus_tag="MB219_000037"
     CDS             complement(41926..43278)
                     /locus_tag="MB219_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCC8425782.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43501..44901)
                     /locus_tag="MB219_000038"
     CDS             complement(43501..44901)
                     /locus_tag="MB219_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCC8425783.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45062..45799
                     /locus_tag="MB219_000039"
     CDS             45062..45799
                     /locus_tag="MB219_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCC8425784.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45924..46403
                     /locus_tag="MB219_000040"
     CDS             45924..46403
                     /locus_tag="MB219_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCC8425785.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46400..47167
                     /locus_tag="MB219_000041"
     CDS             46400..47167
                     /locus_tag="MB219_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425786.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            47397..47966
                     /gene="lepB"
                     /locus_tag="MB219_000042"
     CDS             47397..47966
                     /gene="lepB"
                     /locus_tag="MB219_000042"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCC8425787.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48070..48672)
                     /locus_tag="MB219_000043"
     CDS             complement(48070..48672)
                     /locus_tag="MB219_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCC8425788.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48778..49247
                     /locus_tag="MB219_000044"
                     /pseudo
     CDS             48778..49247
                     /locus_tag="MB219_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            49353..49937
                     /locus_tag="MB219_000045"
     CDS             49353..49937
                     /locus_tag="MB219_000045"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCC8425789.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            49940..51013
                     /gene="prfA"
                     /locus_tag="MB219_000046"
     CDS             49940..51013
                     /gene="prfA"
                     /locus_tag="MB219_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCC8425790.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51006..51845
                     /gene="prmC"
                     /locus_tag="MB219_000047"
     CDS             51006..51845
                     /gene="prmC"
                     /locus_tag="MB219_000047"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCC8425791.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51838..52017
                     /locus_tag="MB219_000048"
     CDS             51838..52017
                     /locus_tag="MB219_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425792.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52039..53064
                     /locus_tag="MB219_000049"
     CDS             52039..53064
                     /locus_tag="MB219_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCC8425793.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53239..54483
                     /locus_tag="MB219_000050"
     CDS             53239..54483
                     /locus_tag="MB219_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCC8425794.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54636..55583
                     /locus_tag="MB219_000051"
     CDS             54636..55583
                     /locus_tag="MB219_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8425795.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55716..56345
                     /gene="upp"
                     /locus_tag="MB219_000052"
     CDS             55716..56345
                     /gene="upp"
                     /locus_tag="MB219_000052"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCC8425796.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56484..56726)
                     /locus_tag="MB219_000053"
     CDS             complement(56484..56726)
                     /locus_tag="MB219_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425797.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56847..56919
                     /locus_tag="MB219_000054"
     tRNA            56847..56919
                     /locus_tag="MB219_000054"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56880..56882,aa:Thr,seq:cgt)
     gene            complement(57038..>57585)
                     /locus_tag="MB219_000055"
     CDS             complement(57038..>57585)
                     /locus_tag="MB219_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCC8425798.1"
                     /translation="PSIISIFSLTITGFIAFTLVFGKKDTKKMFSKPVAPVKNIAKYF
                     FINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFLAIFS
                     SKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKSNSII
                     TSWAVHVIFDFTAILLVVLFS"
BASE COUNT        19130 a        10081 c        11928 g        16446 t
ORIGIN      
        1 attgacaacg ctttcaacga ggtgtattat tcatgtataa attaaaggag gtcagttatg
       61 gaagccacaa tgttgattag tagtgtcaag cgtgtaggca aagttgatcc gcggttgtac
      121 agttcattta ttgaacatat gggacgagca gtatatgaag ggatttatca gccagatcat
      181 ccatcagcta ataaaagtgg atttcgccaa gacgtcattc acttggtaaa agaattgaac
      241 attcccatga ttagatatcc tggtggaaat tttgtgtcag gatttcgttg ggaagacagt
      301 attgggccca tagcaaatag acctagaagg ttggatttag cttggcggac aattgaaacg
      361 aatgaagttg ggattcatga attttataac tggtgtcagc atgtgggggc cgaaatcaat
      421 atggctgtca atctgggtac tcgtggagta gatgcagctc gtaatttagt cgaatattgt
      481 aattttccta aaggaactta ttggagtgat ttaaggcgtt cgaatggtca agagagccct
      541 tttggaatta agacatggtg tttagggaat gaaatggacg gtccttggca gattggacat
      601 aaaacagcgg aagaatatgg acggttagca gaggaaacgg ctaaggcgat gaaactcgta
      661 gatgattcaa ttgaggtggt tttatgcgga agttcgaatc ggcaaatgcc tacatttggt
      721 gattgggagt taacagttct tgatcaagcg tacgatcaaa tagattattt gtctctgcat
      781 caatattatg gcaaccaaaa aaatgatctt tctcattatc ttgcacgttc cttagacatg
      841 gatcggttca tttctgaagt gacagcgatg tgcgatacag tcaaggcgaa gaaacatagt
      901 aagaagcaga tcaacctgtc ctttgatgaa tggaatattt ggtatcattc agcggaacaa
      961 gatgagcaag cagcgccttg gcagattgct cctcctttac tagaagataa ctataatttt
     1021 gaagatgcat tactattagg ctgtctatta atcactttat tgaaacatgc agatagagta
     1081 aagattgctt gtcttgctca attggtaaat gttattgcac caatcaggac agaaacaaac
     1141 ggagaagctt ggagacagac gatctattat ccatttatgc aagctgctgt gtatggacaa
     1201 ggagaggtgt taagcccaca aattcgttca gcaaactatg cgacggaaga ttttgaagaa
     1261 gtgccgtatt tagaaagtat agccgtaata aatgacaaag aacttgtcat atttgcagtt
     1321 aaccgtggag aagaagaaat gaatctaact gttcacttgt ctgaattatc attagaggga
     1381 gtgattgatt tttctgaaat gagtggattt gatacaaaag caactaatct tcctaaaagt
     1441 gaacaagtaa aaccaagtca ttcaacaaat gtaaaagctg aagatgggaa aatcaattgt
     1501 ttattgaaac cgctatcttg gaatgtggtc agatgtcgtt tatcagatga aggaggaaca
     1561 aaaaatgaaa ggtaaaaaat ggttaattgg ctctttgttg gtagctgcaa caggtatttt
     1621 aggagcttgc ggtggcggaa gttcagcaga atctgattct gataaagaag tgactttgac
     1681 tttttggaat gggtttactg catctgatgg tgaaatattg caagaaatcg ttaatgaatt
     1741 taataaaaca aatgataaaa acatcaaaat tgaaatggat atcatgactt gggccaattt
     1801 gaatgaaaaa cttcctacag cgatttcttc taaaaaagca cctgacttta ttgcattaaa
     1861 ttatccagat tttgcacagt atgtagaaaa cggagcggta caaccattag acgatttctg
     1921 ggaatatgat ggagtagata aatctgattt taaagaaacg gctattgaat taggtgaagt
     1981 agaaggtaaa cagtattttg tccccatgca ggtacaaggc atgtatatgt attggaataa
     2041 tgatttgttt aaacaagctg gattagatac tgaacaacca cctactactt gggaagaatt
     2101 gacagaaatg gcaccaaaat taactgatag tagtaaaaat gtatcaggtt ttgtcttcaa
     2161 taaggatgga actgcaccgt tatataactg gatgttagca aatggcggta aattagtgaa
     2221 cgatgattat actaagtctg aatttgcgtc tcctgagaac cttaaaacgt taaaagcgat
     2281 ccaaaaaatg atccatgtag acaagactgg tccagaatcg atttctggcg ccgaaatgga
     2341 caatttaatg aatgctggtc aactagccat tgaaatcaat gggccatggt taaataacgg
     2401 attgaaagca aacgagattg attataatgt gactacctta ccacaaggga gtgattcaga
     2461 caaaaaaaca gctattttag atggcgtagg atatgccatt ccggcaagta cagataataa
     2521 taaaaaagaa gctgtctatg agtttttgaa atattggaat acaactgaaa ttggtaaaaa
     2581 atggagtatc gaaaatggtt ttcctgccta cttggattca gtagcaaaag atgaagaagt
     2641 aaaaaatgat cctattgtat ctgaattatc gaaacaaatg gactatgcag agccatattt
     2701 accaggtttt acccaaatct ctgcgatcaa taacgatatc atcaatccac tgattgaaaa
     2761 attattagca ggagaagatc ctgagacact catgaacgat gcagatgatg ccatcaatca
     2821 attactgcaa aaatagtaca gaactttata caggatagac tgttggcaag atacagatat
     2881 ctgaatgatc gacctagtat ttgcagatag gtgttattgc aaaaatcaca gcgattgttc
     2941 ggatatctta ttcagattcg tttaaaccaa gattgtatat tttatcaaaa tgaaagatga
     3001 ggtggactat atgaacggat tcaccaaata ttggaatcgt cctgatcgtg ctgcgtattt
     3061 gtttttaact ccatcttttt tagttttatt attatttact gtcataccgc ttttaggaac
     3121 atttgctatt agttttacag atttgaatat tttctttaca tcaagaaatt ttgtaagttt
     3181 tgaaaatttc acacgtatat ttagtgatga acgagcagtt aattcgttga ttcattcttt
     3241 atatttcacc gtactcgaaa cgccaacaca aattatcgtt ggtttattag cagcagtagc
     3301 attatctaaa aataccagat tcaatcgttt ttgtcgttcg acattttaca ttccagttat
     3361 ttgttcattg acgtcaattg cgattatttt ttctatgttg cttgatccga atgtaggggc
     3421 gattccttac ttgttttcac aagtcggctt gccgatccct caattttttc gcgatccaac
     3481 ccttgctatg ccgacggtag ccatcatgac tgtttggaag aattttggtg ttactatgac
     3541 gattctcttg actgctattc agggaatctc tccctctttg tatgaatctg ctcaaatgga
     3601 tggtgcaaca aatcgacagc agttttttaa cattacgcta cctcagatcg ttccctcctt
     3661 aggtttttgt attttaacga atctgattgg ttcgatgcaa gtgtttgacc aagtatatgt
     3721 tgcaaccgct ggtggcccac aatttaagac agaaacagct gttcaatata tttacagtcg
     3781 aggatttaca gcaccttatg aattgggcta tgcatcagca ctgtcttcgg ttttgtttat
     3841 cattgttgca actatagcta tctccactaa tgtgtacatg tcacggaaag aaaaacaaat
     3901 gaaataggag ggaaacctgt atggcaccag aaagctcgat tacagttact gtgaatagtc
     3961 gaaaagcagt tagtggtaaa aaaatcattc gaacgattag tatgctagtt atattgttgg
     4021 ttattgcgtt tccttttttg tggctgatta tttcttcttt taaacacgaa aaagacatta
     4081 tctcatttcc gcctagaata tttgcagata gctataccct agataactat attaaggttt
     4141 ggacaacgat ccctttatta gattacatca aaaatacagt gatttttgct ggagggacag
     4201 tgatcacttc tgtattcttt gattctcttg cggggtatgc atttgctcgt atgcgtttca
     4261 aaggaaaatc agttttgttc tatttcgttt tgctgacaat gatgatccca ttccaagtct
     4321 tcatgattcc tctatttatt gaagtcaatt tgcttggcat gcttgatact tatgcaggtt
     4381 tgatcattcc tcgtatgaca actgcatttg gtatcttcat gatgcgttcc ttttttatta
     4441 ctttaccaga ttctttggaa gaggcagcac gcattgatgg attaagtgag tttaatattt
     4501 ttttgaaaat catgttgcca ttgagcaaac ctactctttt atcattaggg attttcacgc
     4561 tgatgaatag ttggaatgac ttgctttatc cattgatatt gacaagtagt tcaaaaatga
     4621 gaactttacc agcagggtta gctttattta cagggcagaa catttctttt tatggacctg
     4681 taatggctgg aacggttatt tcaatgctgc cattactagt ggtttatatt tttgcacaga
     4741 aatattttgt tcaaggaaca gctatgtctg gaatgaaaga gtgaggaaga tgaaaatagt
     4801 tgagactaaa ctttatggac agttgattgt ggtttctgat tacatcttat taggtattct
     4861 ctgggtaatc gtgtgtttgc ctgttattac tatcgtgcct gctacatgtg ctgtttttta
     4921 tgtaatggcg caatggaaag gtgagggatc aggaaatatc atttattttt tctttcaagg
     4981 aataaagcaa cattttggta agaacttgtt ccttagtctg ttaacgatca ttatttattt
     5041 tcttgttaat caattattag aagaacagac aatcgtactt tcaattagtg gatatgtagt
     5101 tttacttatt tattccatgt ttcttttatc ctggatatat gtgatttctg aaaacaaaga
     5161 tatcacattt ttacaaattt ttgaacaaag tgcatggaaa gtattttttg attttttgag
     5221 aaatattctt tgttgcgtat tgttattagg atttgtactg ctcatatttc ttttccctcc
     5281 ttttattttt atctttgctg gtggcgtatg gaaacttgta tctattatct tgactatagg
     5341 aaatgaaagg tagggaaagc tgttggacga agaagtgaaa gtaaaaatca gagaggattt
     5401 tacggtagga gagatcagtt ctcggctgtt tggctcattt gttgagcata tgggaagtgt
     5461 cgtctataca gggatttatg aaccgggcca tgaaacagct gatggtgacg ggtttagaca
     5521 agatgtcctc tcttatgtaa aggaattaga attaggtgtc attcgctatc ctggaggaaa
     5581 ctttacatct ggttatgatt gggtggatac tgtaggtagg aaagaacaac gaccaaaaaa
     5641 aattgattta gcatggcgag gtatagaacc aaattctttt ggactccacg aattttttaa
     5701 atggatcagt catgtacaag cgaaaccgat attcacggtg aatttaggaa caaaaggaat
     5761 tgatgaagca aaaaatttag tagaatattg taattttcca aaaggaacct tctggagtga
     5821 tttaagaata aaaaatgggc aaaaaaagcc ttttggcgtt aaattatggt gtttagggaa
     5881 tgagctagac ggtccatggc aagtgggggc taagagtgct tatgaatatg gtaggttagc
     5941 aaatgaagcc tcaaaagtca tgaagttagt ggatgattca attgaaacaa tcttagttgg
     6001 aagctctacg ccacgtttac catcgtaccc tgaatgggat cgtatcgcat tagaagaagc
     6061 ttatgagaat gtagattatt tagctttgca taactatatt gataagtatg atgaagaaga
     6121 cttgactaaa cctcctaaaa atgagcgtga tgatacagcg acataccttg caaaagctta
     6181 tcgattcgat cgtcaaattg aagaaatcgt tgctacttgt gattatgtaa aaggatattt
     6241 gagaagtgag aaaacaatgt atctagcgtt tgatgaatgg aatgtacatg cccaaccgga
     6301 aaaaagacat agggattttg aagtcggtag tcccatcgat tggtgttact ttactatgga
     6361 agatacatta ttatttggtt cattagggct ggctatttta cgacacgcag atcgcgtaaa
     6421 gattagttgt caatcattgt tagtaaacac aataccttta atactgacag atgctaaagg
     6481 acaagcatgg aggaatccaa cttattatgt gatgcaacac ttatcgaaat atgctaaagg
     6541 tcaagtattg aaacaacaaa tcatttgccc tgtttatgat tgtgaaaaga taaaaaatgt
     6601 tccttatgtg gacagtgtga tcgtagataa cggcaatcag ctgactattt tcttgatcaa
     6661 tcgcacaaat gaacaacaaa agataacgat tgaacatgat ttcaatttgc tagatcaagg
     6721 agagcactac acattaacca gtagcgatct gttggacaaa aatacacgtg aagaaccaga
     6781 aaaaataaaa cctgaaataa actatttttc aatagaaaaa catgacgaat taaatgtagg
     6841 gatagaaaaa tactcttgga atgtaattag aataaagaaa atgtgaggaa atttgttatg
     6901 atgaagacac tttataataa tcctatcgtt cggcaacggg cagatccttg ggtatataaa
     6961 catacagatg gttattatta ttttacagga tcggttccag gctaccaagt gattgaatta
     7021 agaagggcaa agtcactgaa tgaattagaa catgctgcaa gcctgattgt atggcaggct
     7081 catgaagaag gaccgatgag tgaattgatc tgggcacctg aagtccatta tatcaatgga
     7141 aaatggtata tttactttgc tgcaagcaat cataaaacta tacgtgatga atctcatcat
     7201 caccgcatgt tcgtacttga aaatcagcat atagatccaa tgaatacaga ttggatagaa
     7261 aagggacaaa taaaaacgaa aagggaaact ttcagtttag acggaacaac tttctcttct
     7321 caaggaaagc tatattatgt gtgggcccaa caagatcctg aaattactgg gaattctaat
     7381 ctttatctat ccgaaatgga taatccttgg acgttaaagg ggaagcagat cctcctttct
     7441 atacctgagt atgaatggga aaaacggggt ttttcagtaa atgaaggtcc agcagtcctt
     7501 actcgcaatg ggaaaatatt cattacgtat tctggtagtg caacagatga aaactatgcc
     7561 atgggcctgc tgtgggctga tgagcacaag aatcttttat taaaagaatc atggcataaa
     7621 ttatcggagc cagtattcgt ttcttctgaa aaaaataagc aatttggtcc tggtcacaac
     7681 tcgtttacgg tttcagaaga cgggtgcttt gatattttga tctatcatgc tcgaccagag
     7741 aagaaccaaa aaggggatcc gttagacaat cctaatcggc acgcgaacgc gcaatgcttt
     7801 acgtggaatg agcaaggatt tccagaattt ggtgaacctg ttccgtatgc cttatgagca
     7861 caagaaacct tgatttttgc ttgaataaac cttgcagact cgttatgata tatgtacgta
     7921 caaataaaaa ggggcaaggc aaatggcaaa atatcaagat attgcagaag agataaaaca
     7981 aaagatcctt cgtgaggaat acgaagtaaa tacaaccatt ccctcagaat tcaagctgca
     8041 agaaatatat gacgtcagca gacatacgat tcggcaagca atcgctgtgt tggtaaatga
     8101 gggctatctg cgtaaagaaa aagggtcggg cacatatgta gatgacagct ataagaaaga
     8161 aaaaacacca ttgaacagaa caaaaacgat cggtgtcatc accacatatc tctctgatta
     8221 tatttttccg tcgattatcc gaggaatcga aaatgaatta cgagaatctg ggtattcatt
     8281 gcttttagcc agtacgaata acgatcacga attggagaaa gagtgtttga agaaaatgat
     8341 tgcttatggg gtagatggtt tgatcgttga gcctacgaaa agtaatcagt ataacccaaa
     8401 cttagcactt tatgttgctt tgagagaaca aggcattccg atggtgatga tcaatgctgc
     8461 ttatgaagag ttaaatgcct cttatatttg tttagatgat gtgaaagtcg gttttatggc
     8521 agcagaatat ttgataaaaa aaggacatca gcgtctgttg ttcatcacaa aaatcgacga
     8581 tcttcaagga aaatacagga tgaaaggttt tatcagcgcg tgtgaacagt ataatgtgcg
     8641 ttttttgacg gaagatatca ttactttcac cacggaaaca cgagaaacaa tcgctgaaaa
     8701 aacagtacaa cggttgaaag aagaacaagc tttagatggc atcatttgct ataacgatca
     8761 aatagctagt ttaatgctag ataagttgac agaaaatggc tatcatattc cagatgacct
     8821 atcgatcgta ggaacagacg attcttcttt aagccattta gggaataaaa aattgacaac
     8881 attgacgcat ccaaaagaaa agttgggaca agatgccgca cggtggatcg tcacaacgat
     8941 tgaaaaagga caggcacagc caaatattct ctatgaacct gtactagttg aacgggaaac
     9001 agtgaaagaa aacgaaaaat aagagacggg tttttaccgc ctcttattgc tcctacggtt
     9061 actcgtcgca atgtttgtga gagcttttgt catcgctcaa ggctgaacgt ttctttcacc
     9121 acctctccac taaacggtgt tctcctacca actggtcaca agtcacaata ttcatcaaac
     9181 atgagaagct gttttttgaa attgttcctt gtgactggaa actaagcggt tccttcttat
     9241 ttcttgaagc tgaccacttc tgtcacaacc tctttaagaa tgatggatag aaatacgttt
     9301 aggataagtt tttttagatg cactttgttc gatttcctct aaagatttcc cacgagtttc
     9361 tggtacgatc tcttgaataa ataaaacccc taaaaagcag atgatcccaa aaatgccgaa
     9421 aacgatttct tgagacatag aagcagtcat cacaggaaaa agcaagccaa ctaggaaaga
     9481 accaatccaa ttaaaagaag aggctaggcc agatgctcgc ccgcggacag ctagtgggaa
     9541 tatttcgcct acgattaccc atgtcagtgg agcccatgtg caagaataaa atgcgacata
     9601 aatacataaa aacaagagaa tcaacaacga atttgtatga gcatccagta cagttcctaa
     9661 aacagctggt aggatgaaag aaagtcccat cacgctaccg cctattttca ataaagttcg
     9721 ccgattgaac ttgtctgcaa taaccaaaaa gagcaaagaa ccagcaacta aaatgattcc
     9781 ttggatgatc ggccacatca atgcatcgct cgctgcatgc ccagtcgctt tttctacgat
     9841 cagcggaata tagtaaaaaa tagcattagc tccttgaaat tgctggaata ctgctacacc
     9901 aataccggca atgactaagg aacgatattt ttctaaaaac aaagtatgcc aagaagttcc
     9961 ggttgtcttt tcttgtttga cattcagctg gatttgttcg aattcctgtt cagcttcttt
    10021 tggtgtccga atatagttca atactctttt tgcctcttcc aagcggccag attgcataag
    10081 aaaacgcggt gactctggta gagctaaaac accgaaaaat aaaatcagcg cggggacagc
    10141 agcgagccct aacatcacgc gccaagccat cgtttcaggc aaccctttta gcaaataatc
    10201 tgcaacatag gaaaggagca tgccagaagc aatcatcact tgattgatcc ctgacaatct
    10261 tccccttagt ctagcaggag ccatttcaga catataagca ggaactaggg cagatgcagc
    10321 gccaacagct aaacctagaa gaatccttgc tgcaatcaga aaataagaac cattgtgagg
    10381 ggcagatgca gaaaggacag aaccagccat aaaaataagt gcagaaagaa gaatcatttt
    10441 tcgtcgtcca agcttatcag aaagaagacc agataaagag ccgccaagga tcgcacctaa
    10501 catgaccgaa gacgtgatcc atcctatcaa agaagcttta cctgccaaac cccaatcatg
    10561 ctgtaaaaaa ggtaaggcgc cggtcataac accaatatca tatccaaata gaatgccgcc
    10621 aaatgcgcca aaaaaataaa taaacgatga ggaaactttt ttttcttttt tcataagtat
    10681 tctctccttc tagaatttcg agaaggcggt tgatcagatt ttgtaagcaa tttcatttta
    10741 taacttatgt acgtacaaaa caagtgtttt ttattgtatt cttgtaaata actgcttgac
    10801 atgtacgtac aaaagtatac aataatcatt gtaagcgcta ctaaaaagac ggtttgatca
    10861 ggtttgacga gaaggaggga gttaaatcat tatgaaggaa gtaaatgcgc aaagtatgtt
    10921 agatgtcata gatggtcgta catctttagg gattgagttt ggatcaacta ggatcaaagc
    10981 tgtattgatt gattcatctt ttactcctat tgccgctggt agttatgagt gggaaaacaa
    11041 actagaaaat ggtatctgga cctattcatt agaagatatt tggaaaggac tgcgcgtcag
    11101 ttaccgaaaa atggcagcag aagtattcga aatctatggc gagaatctta cgacaatcgg
    11161 gtcgattggg ttcagtgcaa tgatgcacgg ttatctagca tttgatagca aggaacagtt
    11221 actagtacca tttcgtactt ggcgaaatac aatcactgaa gaagcagaac gaatattaac
    11281 ggaaaaattt caatacaata ttccgcagag atggagtatc gctcatttgt atcaatctat
    11341 tttgaataaa gaagaacatg tgaaagaaat taattttttg acaacgcttg caggttatgt
    11401 ccattggcag cttactggtg agaaagtact aggcgtagga gatgcatcag gcatgtttcc
    11461 tatcgatcct tcaacgaaaa cctaccatca agaaatgaaa aatatttttg gtcagttaat
    11521 cgaaaaagcg cacgtagcga ttgacttgga tcacattttg cctaaagttc tagtagcagg
    11581 agaagaagct ggcaagttaa gcgaatcagg ggcttatctg atcgacccta cgggaaattt
    11641 gagaccagga atcccggttt gtccgccaga aggggatgca ggaaccggta tggtcgctac
    11701 gaacagtgta gccaaaggga cagggaacat ttcggctggt acttccgcct tcgctatgat
    11761 cgtattagaa aaagaactag aaaatgtcca tccggaaatc gacatggtca ctacaccttc
    11821 aggagaacta gtaggcatgg ttcatacgaa taactgctct tcggatatca atgcttgggt
    11881 cagtattttt gaagaattta ccaattcttt gggtatggag ataagcagag ataaattatt
    11941 cacggtatta ttcaacaaag cgctagaagg cgatacggat tgcgggggat tactttctta
    12001 tggttatttt tccggtgaaa acattacagg agtaaatgaa ggaaggccat tatttgtacg
    12061 tacaccaggt agccgttttg atttagcgaa tttcatgcgg atccatttag ccagtgcttt
    12121 tggtgcaatg agaatcggaa tggatatatt gaaatctgag aatgtccaaa ttgatcgttt
    12181 agtggggcat ggggggattt ttaaaacacc agaagttgga cagcggattc tagcatctgc
    12241 tatggaggca ccagttacag tgatggacac agccggagaa ggtggagcgt ggggaatcgc
    12301 gttattagca gcctatatga tggatcgaca tgggaagtct ttagaaaact tcttggcaga
    12361 agacgtgttt ggacaagacc aaggaatcac catcacacct tcagcagaag aaatagcagg
    12421 ctatcagata tttatgagac gttaccgtga ggggatcgac attgaaaaaa cagcgattca
    12481 tcaattgcaa gaaaaaggag tgatagaaaa tgctggaaca actgaaagaa gaagtatacc
    12541 aagcaaatct tgacttgcct aaacacggac tcgtcaaata tacatgggga aatgtgagcg
    12601 cttttgatcc tgaaacacga tatttcgtta tcaaacctag cggagtcagt tatgaagaga
    12661 tgacagcaga cgacatggtc gttgtggact tggacaatca tatcattgaa ggtaaactca
    12721 atccctcttc ggatacgcct actcatgctg ttttatatcg ttcatttcct caaatcggag
    12781 gaatcgtcca tactcactcg acatgggcga cgatctgggc acaagcagga ttggatgttc
    12841 ctgcgatggg gacgacgcat gcagatactt tttatggatc ggtaccttgt gctcgatttt
    12901 tgacacaaca ggaaatcgac agcgggtacg aatatgaaac aggaaaagtt atcatagaaa
    12961 cattcaaaga acgcaaaatt gatcccttag caattccagg ggtactttta cacggacacg
    13021 gtccatttac ttgggggaaa gatgcgcaaa gtgcggtaat gaacgcagtc gtactagatg
    13081 aagtatgcaa gatgaattta tttaccagac agctcaattc attttcagaa gagctgcctc
    13141 aacggatttt agataaacat tatttacgaa aacacggaaa aaatgcgtat tacggacaaa
    13201 acaaataaaa ggagtggcta attatgttga atattggaga aaaagaattt tggtttgttg
    13261 taggatcaca gcatttatat ggggaagaag cactgcgaga agtaaagaaa catgcacaag
    13321 aaatggtcga tgaactgaat gaaaatggcc aattgcctta tccaatccgc cttcaagaat
    13381 tagcagttac tgctgatacg atcacaaaaa tcatgaaaga ggtcaattat cgagaagaag
    13441 tagcaggtgt gatcacttgg atgcatactt tctcaccagc aaaaatgtgg atccgcggga
    13501 caaagcttct acaaaaacca ttgcttcatt tagctacgca atataacgaa agcattcctt
    13561 ggaaaacgat cgatatggat ttcatgaatt taaatcaatc cgctcatggt gaccgcgaat
    13621 atgggttcat caatgctcga ttaaataaac aaaataaaat cgtcgttggc tactggaaac
    13681 gacctgagat ccaaaaagag attgctgatt ggatggatgt tgcagtagcc tataatgaaa
    13741 gttttggaat aaaagtggct cgttttggtg acaacatgag aaacgtgggt gtcacagaag
    13801 gagacaaagt ggaagcacag attcaatttg gttggactgt tgattatttt ggtatcggag
    13861 atcttgtaca agtaatcgat cgcgtatcag acgaagaagt cgatcaatta tttgaagagt
    13921 acaaggaatt gtataccttc gactatggtg attatgaaga aaagacatgg gaggaacatg
    13981 taaaagtaca agctcagcag gaaatcggta ttcgccgttt cttagaagaa ggaggctata
    14041 acgcttttac gactaatttt gaagacctgt atggaatgaa acaactacct ggactagctg
    14101 tacaacgttt gatggcagaa ggatacggtt ttgctgggga aggcgactgg aaaactgcag
    14161 cgattgatcg gttgctgaaa atcatggctc gagggaaaga tacaggattc atggaagatt
    14221 atacttacga gctagctagt ggccaagaag caatcttgga atcccatatg atggaagtag
    14281 atccgacgct tgctgcgacg aaaccaagaa tcgttgtttc tcctttatca atgggagata
    14341 gagaagatcc ggcgcgtctt gtatttgatg ggaaagcagg agaaggggta gtcgtatcga
    14401 tggcagattt cggtacgcat tataaactgc tgatcaatga agtagaagcc tttgaaccaa
    14461 cgactgaagc acctaattta cctgttgcac gtgtgttatg gaaaactaaa ccgaatttcc
    14521 atgaaggtgt ccattcatgg attcaggcag gtggcggtca tcataccgtt gtctcgctaa
    14581 atctaacaac agatcaaatt gaaacgtggg caaaattagt tgagttggaa acagtggtta
    14641 ttagataaaa agaggttgtg aaaaagccac ttagttttaa gaaataagaa gagattgacg
    14701 aaatttgctt ttcaactttt ttgaaattta gtcttattat caatgtcagc taacggaaca
    14761 ccatttatca agatatctca ccctcaatat tttgctctcg aaaataaggc ttatggcagt
    14821 aacttgtcat agccttattt ttatcactca gcctactctg ctctaactgc tctttttgct
    14881 tctaggaagt caaatggtaa acagttgtct aattgcagaa atttcgagat acttgttagt
    14941 aaggaggggg ttttatgtat cacgttcatt tgattgatgc tattccagaa caagagatag
    15001 aaaagttaca aggggaatat ttcaaaataa tagatgattt gccagccgaa ggaatagtta
    15061 ttcgtagtac gaaagtgaaa gatgaatggc ttacaccaga tttgctagca atttcaagag
    15121 ctggtgttgg ggtgaacacg atcaacgttg aaaaagcttc tgaaaacggg acgatcgtca
    15181 tgaatacacc aggtgtaaat gctaatgcag taaaagagct tgttttgtgc tgtttgctct
    15241 tatcttcccg tccgataatc gaagcaagtc gtatggtaca aacactaaca ggtcctaata
    15301 ttttagaaca ggcagaaaac aaaagaagtg cttatgtggg acgagaattg caaggcaaaa
    15361 ccatcggtct tcttggtctt ggagcgatag gcacaaaagt agcgctaagt tgctatagct
    15421 tagggatgga tgtcttgggt tattctatcc gcgatgccca gttggattat gttcggcaag
    15481 cagatttaga aacagttctt tctacctcgg attacatcgt agtcatgctg ccgttgacag
    15541 aagatacaaa aggattgatc gatcaggcga atatagaaaa aatgaaaaaa gatgctgtac
    15601 ttatcaatgt aggaagaagt gaaattgttg ataaatatgc tgttatgcaa gcattggaaa
    15661 aacaacatct ggctaaatac ttgactgatt ttccagaaga agagttctta gaaaatgacc
    15721 gtatacttat gttgcctcat ctaggggatc gactcaggaa gctttggcag acagcggacg
    15781 tttggcagtt gaagccttaa aagattatct tttgttcggt acggttagag aagcggtcaa
    15841 ttatccatcc gcacgaatgt ttttccaagc gccgtttcga ttcactatat tttatcaaaa
    15901 gaaaaccaat atattagcag agatattttc tctgctgaat gagaacgagc tagcgatcgc
    15961 agatatatcg cgtaaccata agaatgagca tgtctacata ttgattgaca tagacagtac
    16021 tgattttgaa cagctcaatc aagtgaaaca acagctagaa aaaatttctg gagtacgaaa
    16081 ggtccgctta ttaaagaaac cagaaaggcc ataaagaaag agaatatttg gatttcatta
    16141 attcttcttt ccgttcttta ctttcgaatt tcgtacaact aaaataagag aagcaacatt
    16201 cttttattga tagaaatgaa ggagcaagca tttgaaaaga tatataagca gactttcgac
    16261 caaacagaaa aagattttaa aaggaacagc agcaatcatc agtatctttt ttcttgttgt
    16321 ttttagtaat ttatttttgc agtggtgtca aaatgatcta tccgtagacc ttgcgttgaa
    16381 atttgctttt tcttggcata cggaaaaatt cttcttagcc tgtctcgtct tgctgattat
    16441 tcttattttt ctgattgcac tagcaggatc tgtcccttta ggttccctta cgtatgtcgt
    16501 agccatcggt gtattaggtt ttgcaaatta tatgaaaatg agttatcggc aagaaccgat
    16561 ttatccagac gatttgaaga tgatcactga aatcggattg ctgaaagata tgaccggcac
    16621 tatgctgttt acagtgatct tagctgccgc aggaaccgtg ataggattgt tttgctggta
    16681 catgtttcga agcctgaaaa aaggaaggcg attccaattg atccgattaa cgacactgct
    16741 tgtggcaatt ggactgctag ggtatatcag taatttcaac aatcccgata atttgttgcg
    16801 aaaagcatat aataaaacgg cactatggat accttacagc cagaaaatga actactataa
    16861 cactggtttt atcggtgggt ttttgtacaa tctgaaggta gaaccaatgg atgaacctga
    16921 aggatattcg aaagaaaaga tcaaagaaat cactgaaaaa taccagaagc tagccgatga
    16981 aaaaaataaa actgtagagg aagagtcgcc gaacatcgtg ttcgtgatga gtgaaagttt
    17041 ctcagatcct agtcgattaa acggcgtaga agtatctggt gagccattgg cagattatta
    17101 tgaagtagct gatcaaacat atagtggcaa tatgctttct caaaactatg gcggtggaac
    17161 agcaaatatc gaatttgaag cattgactgg tttttctatg gcgttattta atgcgcaact
    17221 caccacccct tacacgatgc ttgtaccaaa aatggatcag cttccttcta tcgtttcagc
    17281 gttaaacgca caatcatacc agacgacagc gatccatcca tacaatactt cgatgtataa
    17341 aagagaggat gtttatcaga cattaggatt tgaccagttc atcagcgagc gtacgatgac
    17401 ctatacggat acgatagaga ataatccata tatttctgat gagtcagctt acaaagaagt
    17461 aatgacactt ctcaaggaag aaaaaacacc tcagttcatt catttagtca cgatgcagac
    17521 acatatgccc tacgatggaa agtatgataa tctagactat cctgcaaaaa cttcagatgg
    17581 aagtgggaca cttgacttag agaattatct gcaagatatt tcctatagca gcacggcact
    17641 gaagcagttc acggaagaat tgaagaatct ttctagaagg actttagttg ttttttgggg
    17701 ggatcatctt ccagggatct attcagatac gatccaagca aaaaatgaca aacaaacatt
    17761 gcatgaaaca caatttctta tgttcgacag taaaggagaa ttagaaaagc aaacgactca
    17821 cgatgcaatc accagtccgt tttattttgc agcgaatcta atggaacaga cgaatcagac
    17881 aacaaatggc ttttatcagc tgcttttatc attagaacag gaacttccgg cgtttgaacg
    17941 agaactgtac tatcaaaacg gacagtggta taaagaagcg caattcaatc gctcgcaaca
    18001 agagatttac gacgaatatc aattgatcca atatgacatt gtcgcaggga aacaatatag
    18061 tttagcagat ggtttctttg agcacgaata agaatggcga gctgtataga aaccattaaa
    18121 ttttttcaag tacttgtcat ttgaggataa aaacaagtag aataaataat aacaacaatg
    18181 gagaaagtag gaaaacgaac atgttaccga atattggcta ttatgtgcag tccatcgatc
    18241 aaatcgtcaa ggaaacagaa gcaatcggag aaggaatgca tccgaaatat gaagtgatca
    18301 gaaacgcaat tgatgaagga aaaacaagtg aactggacaa atcgactctt gaagagatca
    18361 tcctgttatt tgaagaagga acaaaaaaat atcgggcaat gcttatgaaa gtcagtactt
    18421 tgcgtccacc agcaaaagca atgggcattc ataaaaattt tgaaaaagct tatctttcat
    18481 atgtagcagg ctgtgaagaa atgatccaaa gtatcgatct tgaatcaggg gtagatgaag
    18541 atctattcaa tacatctgaa gcaaaacaag accaagcgac agaccaattg acagctgcca
    18601 tccaaaaaat gactaatttg ttgttgaaaa aataaaagaa atctttgata acaataaaaa
    18661 ctagagttat ctaatataaa tgtaatatta cctttatgat agagtgatca aaatccttta
    18721 tgataatgac atacctataa ccaacaacaa ccttgtactt actcaaaatc gagtaaatac
    18781 tccttttccg tcgagtctaa ccatctcggc ggttttttat ttgtagaaaa gaacatcagg
    18841 atagacggaa aagtcttgtc ctgatgttct tttcagatag tatggaaagc tgattagttt
    18901 tctacattct gtacatattg ttcaaagtag ttctgtaaat cggctttgag tttctgatat
    18961 tcctcttcgc tatactcttt atcttcgatt ttcccatcaa agaaaggaat atttagttct
    19021 tcccgtagtc tgtctaatac gtcttgctga ttcatttcat tctccttttc ttactaacga
    19081 tcaaaataaa cacagacaca ttctggtaca tagacatctt ttttcagtaa ttccagtaat
    19141 cctgtttcag gatgacggcg atataatgtc agattatcgg tgttctgatt ggcacacacg
    19201 acaaaattat tgcttggatc tagtgcgaaa tcgcggggaa aatccccttc tgttgagatg
    19261 atttgcttgt tttgaatgtg ggaaccatct tcaaaaactg cgaatacagc aatcgagtta
    19321 tgtcctcggt tagaagcata aaggaacttg ccatcatttg aaatgcggat agctgcccct
    19381 ccattttcac cgtcgaattc ttctggcaag gtcgatattt tctgtaattc ttcaaaagca
    19441 cctgtttctt tattataaga aagcacgcgg actgtgctgt ctaattcacc aaataaataa
    19501 gcgaattgtc catttggatg aaagacaaga tggcgagggc ctgttcctgg ctctgcagtg
    19561 aaggtagtta caagattcaa tttgccgttt tcggacacat cataagtata cacacgatct
    19621 gtaccaagat cacatacgac taatcgttgg tcaggagtca gatccgtata gtggacatgc
    19681 gcatgatctt ggtttttatg aggacctgtc gcttcttgat ggaatattga atctgctgct
    19741 ttcagttcgc catctcctaa tatatggtag acatttactt cacctttatg atagttcgcc
    19801 ccgtatagta attgacgtgc ttcatcaacc gctacataac acaaaggagc gccagactct
    19861 gttaccgcat tcaaaaaatg aaaatcctta tcgtacgcac cggcaccacc taatccatcc
    19921 acactagtaa ctgtgtaaag attgccagct ttactttttg ctacataggt agggctattt
    19981 tcttcaacag ctaatgaaag gttggctaat tcttcttttt ctgtatcaag gtcgatcgta
    20041 tagattcctt tactttcgcg acgcgtatag gttcctaaaa ttatcttttc gatcatatga
    20101 gctgattcct cctaattgta atgaaatagt tttcgttttt attctatcac ataattattc
    20161 cacacggcta aagaatgaga atgtgttata ctgtttttga aagcaattaa aggagagagt
    20221 gcaatgattc aggcaaagat tacagaaatt gggacagaag caataaacaa ggaagaaccc
    20281 atgctgattt tgttcgataa aacagcaaca gctgccttac gaaaatattc ggtgattcaa
    20341 gaagtatcaa aagacgaacc tttttctttg aaagcaggcg gaacaattgc ttttgatgat
    20401 caggtatata cgatcgatta tgtcggtccg atggccaatg ggaatttaaa gacagtagct
    20461 cacgtatcat tgatttttga tgcttgtcca gaagaaggac aaattgcaaa tggaatctat
    20521 cttcatccgc atagactgcc tgctattggt ttgggaagcc aaatttttta taactaacag
    20581 gaggaattat ggaaggaaaa aataagaaga acagaccggt atcgtggttt tggcgctggt
    20641 ttttaaataa tcaagtagtc acagccttac ttgttgtatt gttaatattg ctgatcgtct
    20701 ttttatttac aaaagtttca tatttgtttg caccaatctg gcaattttta gcaatcgtag
    20761 gtctgcctat cattttagca ggaatcttgt attacttgat gaatccggta gttgattact
    20821 ttgaaaagcg aaaagtacca aggctttaca gtatcattgg gctgtttgtt ttggttgttg
    20881 cactaatcgc atgggggagc gtagtgatca tccctaagat tcaggaacaa acagtgagct
    20941 tcatcagtaa cttccctcaa tatgtagata cgattgataa taaattaacc gagattttgc
    21001 gggacccgct gttcaaccaa tttcgcgaac agttggaaac agcaggtgat aagttcatga
    21061 attcggcagg cgatatgatc caagatatat ctaagagtac agttcagagt cttggaagtt
    21121 ttgtcggcgc ggtagcgact attttagtgg ctctgctgac gatgccgttt atcttatttt
    21181 atctattgaa agacggtaaa caattagcac cttattttgt caaattttta ccgacacgca
    21241 tgcaaaaacc gacgttgaat gtgctgagtg aaatgaatag tcaagtttct tcttatataa
    21301 gaggacaact aactgtggct tttgctgtgg caatcatgtt tatgattggt tttgcggtca
    21361 ttggtttaga gtatgctgtc acgttgggga ttattgcggg ttttttaaac ctgatccctt
    21421 acttaggatc atttttagcg atgattccgg ctatcttttt agccattgta gcaggtccat
    21481 tcatgatcat caaagtactg atcgtttttg cagtagagca gacaatagaa ggacgcttga
    21541 tctctccatt agtactaggg aatcagttag caatccatcc agtaacgatt ttattagttc
    21601 tgctgacttc tgggaaattg ttcggaatca tcggtgtgat cttggggatt cctgtttatg
    21661 cagcagctaa agtagtgatt actcatgttt ttgaatggta cacaacaatc tcttctcttt
    21721 atgaagaaaa tccgttgaaa aagcagaaag aataacaaaa agagacagat gggaaaatat
    21781 ccaaggcaca aatgctgctt tgaagatttt ttcctctgtc tcttttgtat gcatttatct
    21841 caatttttaa aatgatttac ctaaagcagc agctttatct aatgcatttt gcatcaactc
    21901 atctgcacgg tcagggtcat aatcgattcc ttcgatgaat agctgatcta cttgatcgat
    21961 gcctacaaag tttaaaatac cttttacata ttgagaggca aagtcttgtc cgttgtatac
    22021 accgccatta gattggatgt gcagtgcttt tttacctgtc actaatcctt ctggcccgtt
    22081 ttctgtatag cggaacgttt ttccagctac attgacggta tcgaaccaag cttttaattt
    22141 tgttgggata tttagattcc ataaggcgtt tgcgataacg attttatcag cagcaaggaa
    22201 ctgatcagtc aattcattga aacgagcaat tttttgttgt tgactttctg ataaggaagt
    22261 gaattctgca ccagcacgta atgcttccca acctgataat aattcttcat cgatttccgg
    22321 aatatcatcg cgataaagtt ccaaaacagt cacttcatca tctggatgat tttctttata
    22381 acttgttaaa aaagacgtca atgccttaac tgtgcgcgat tcttctgcag ttaggggatg
    22441 acctttgact actaaaacag atgccatatg cttaccttct ccttttattg ctatgattgt
    22501 tataactatg actaacaaaa tcagtttact cgaaaaagaa aaggctatca agtcatattg
    22561 ataaggttga agaataaata actaaaaaaa gacagggtat aaaagaggct gtgacacgat
    22621 tttttgtcac agcctctttt gaatctaaca agcaatattc aagcaacact cgactgaaga
    22681 gcttccatcc caccaatgat ataaacagca cgaaaaccat tagcagtaag ttgatcggct
    22741 aatgcttctg atcgcctgcc tgtatagctt agaatataat aaagtttctt tggatctaga
    22801 ttttttaatt gtcttggcaa gatagcagcg gggatagaaa gcgagtggct taagtggttt
    22861 tttgtgtaat ctaaggtatt tcttgtatct aaaacgatgc tgtcagcatc gagaggcaat
    22921 ttattgaagt cttggacact aattgattgg gccatttatc tcatcctttg tttagtatgt
    22981 ggctatttta atgggcaact ctcattaatt caaatggaag gattcctctt atcacttttt
    23041 taatgtatcg gatgataatg aaaaaaacta tgcaaatgtc ttgcaaaaca ggtaggaggt
    23101 gttataatga catagcgtca tatgacaatg tgtcatcttg aaagaaagga gaatccatat
    23161 gccgacacag acatttttcc atttgcctaa agaaaaacaa aaaagactaa tcgaggctgc
    23221 gagaatcgag ttttctagag tacccttaaa agaagcttcg attgctaata tcgtcaaatt
    23281 agcggatatc cctagaggca gtttctatca gtattttgaa gataaagagg atctttattt
    23341 ttattacttt gagacaatga gacgtgacag ctctaaagat atgttacaac taatgaaaga
    23401 atccgatggt gatctattca aaggattcga atcctatttt tctaaaatga tttatgagat
    23461 tttggaaggc gagaatgctt ctttttatcg taacttgttt atgaacatgg attaccgtgc
    23521 ctcaagaaaa gtcgctcctc atacgagtcg ccaaaaaacg cctcacgatc cacataaaga
    23581 acacaaacaa gatgcaaaag agttgcttga attgatcgat cggacgaagc tgaaagtaaa
    23641 aaatgatcat gaattggaat tactgatcca attagtgatg aatactgtat ttacaacaat
    23701 tgcgcattcg tatcgcttat tttcaacatc tgaagattac tcagtagagc aagcaatcag
    23761 tgaattcaat ttgaaacttt cttggctgaa aaacggagta tataaataga aagaagggaa
    23821 ctagatgcta aaaatcgtaa aacggatctc cttgacttca gcaattgcag ctgcagtttt
    23881 catggtgatc caagtactgg cagatttata tttgccgaca cttacctcca atattattga
    23941 taaaggggta gcgcaaggag atgtcgatta tatttggcat acaggttttg tgatgatcgg
    24001 cttttctttg atcagtatct tagctgctat cgcaaataca ttttttgcta ctcgtgaatc
    24061 acagaaactg ggtaagcaat tgcgtacgga tgtctataaa aaatcagaga gtctgacaaa
    24121 agatgcgttt gataaatatg gaacggcctc tttgatcaca agaacgacca atgatgtgac
    24181 tcaaatccaa atggttacac aaatgttttt acgtatgatg atcaatgcac caatcacgtt
    24241 gattggcgcg agcatacttg cttatcaaaa agatcatcag ttgacaaaaa tcttcttagt
    24301 tgtcatccca gtaatgatta ttttgatcgg cggaatcatg tactttgctg ttccattatt
    24361 caaaagtatg caaaagaaaa ctgaccgttt aaacctggta tttcgagaag gattgacagg
    24421 ggtcagagtg attcgagcat tcgataagac acgctttgaa gaaaatcgtt ttgatttagc
    24481 gaataaagat tatacaaata cggcaatcaa agttaatacg atcgtcgcat tgatgatgcc
    24541 aatgatgaca ttgatcatga gcggtacaaa cgtagcaatc acttggtttg gtggacacta
    24601 catagcagat atgactttgg aagtcggtaa tctgattgca tttatgactt atgcgatgca
    24661 gatcttgatc agctttatga tgctttccgc tatttttatc atggtgcctc gtgcacaagc
    24721 ttcagctgac cgtattaatg aagtgttgga tgaaaaaatc ggtatccatg accctgaaaa
    24781 tcctaaaaca gtttcttttg ctggaaaaaa cgcaacattg gccttcaatc atgtcaatta
    24841 tcgatatcat ggagcagaaa aacttgcatt ggaagatatt gatttccaag ccaagtcggg
    24901 cgagatcgtc gcaatcatag gggggactgg ttcaggtaaa acgaccttag tcaatttgat
    24961 ccctcgatta tatgacatcg aatctggttc gatccaaata aatggaacgg acattagtga
    25021 catgactcaa tataatttgc gagaattaat gggctttgtt cctcaaaaag cagttctgtt
    25081 ctctggaacg atccgagaca atatgcagta tggtaagccg gatgcaacag atgaaatgat
    25141 ttggaaagca ttagaaatcg cacaagctaa agattttgtt tcagagatgg aagatggctt
    25201 agatagccat gtggaacaag gaggaggaaa cttctctggt ggacaaagac agcgtctggc
    25261 aattgcacgg gcattagtca aaacagcaga tatctatgtg tttgatgatt ccttctcagc
    25321 gttggatttc aaaacggatg ccaatcttcg ccaagcattg aaaacgaata tgaaagaaag
    25381 tatcaccgtt ttagttgctc aacgagtaag tacagtaatg gatgcagata tgatccttgt
    25441 gctagatgaa ggaaaactgg ttggcaaagg aacacatgaa gaactattag caacaaacga
    25501 aacttatcaa gaaatcgttc attcacaact aagagaggag gacttagcat gagtaaaaca
    25561 aacacacgtc cctctcatgg accagcaggc ggtccaggac atggtggacc tggcagaaat
    25621 ctcggtgtaa aaggtgaaaa gcctaaaaat ttctggggaa cggtacgccg attgtttggc
    25681 tatatgtcta aacgtatggt tgcgatcatc gctgtatttg tactagcaat agcagccgtg
    25741 atcttccaaa tccaaacacc aaaggtcttg gggaaagcaa ccacagaaat attcaaaggt
    25801 gtaatggctg gcgctcaaca gatgcaacaa ggccaacaag taacaaaatt tccgatagac
    25861 tttgataaaa tcgcgcagat cattgcgaca gtaatcgtga tgtatttgat ttctgctgta
    25921 ttcaactttt tacagcaatt tattatgaca cgtgtttccc aacgaacagt ttatgaatta
    25981 cgtcatgatt tggaagccaa aatgaatcgt gtaccgattt cctattatga tacacataca
    26041 aatggggata tcatgtctcg agcaatcaat gatatggata acatcgcaag cacgctgcag
    26101 caaaatttga ctcaatttgt aactagtgca gtcacattga ttggggtcat cgtgatgatg
    26161 ctttcaatca gctggcaatt gacgttagtc gcgttattga tgatcccttt aagcttgatc
    26221 atcgtgatga tcgtagcgcc gaaatcacaa gtgtttttcg cagatcagca aaaaagttta
    26281 ggtttattga ataaccaagt agaagaaaca tatggtggtc ataccattgt taaaacattc
    26341 aatcatactg aaaaagacca ggaagtattt gaaaaagaaa atcagaatct gtatgccgct
    26401 ggttggaaag ctcaatttat ttcggcaatc atcatgccgt tgatgaattt cgttaaaaat
    26461 ctaggctatg tgttcgtcgc tgtactaggc ggagtaaaag ttgcaaatgg gaacatgaca
    26521 ttaggggatg ttcaagcgtt cttgcaatat acaacacagt tctctcaacc aatcactcaa
    26581 cttgcaagtt tgatgaacac tatccaatca acagttgctt cagccgaacg ggtatttgaa
    26641 gtgttggatg aagaagagat gtccaatgaa aaatctggcc ttccagaaga aaaagataca
    26701 ccatacaaag ttcgttttga aaatgttcaa ttcggctaca caccagataa cttgctaatg
    26761 actgatttta acttggatgt caaacctggt gagatggtcg ctattgtagg tccgactggt
    26821 gcagggaaga cgacactgat caaccttctt gaacggttct acgatgtttc tggaggtagt
    26881 attcgttacg atggtgtaga tacccgtgat ctttcacgtg atgaattgcg cgcacagttt
    26941 tcaatggtac tgcaagatac ttggctgttt acaggaagta tctatgacaa tatcaaatat
    27001 gggaacgacg atgcaacaga cgaacaaatc attgaggctg ctaaagctgc tcacgtagac
    27061 gattttgttc gtaaattgcc agaaggatat aacactgtat taaacgagga tgcaagcaat
    27121 atttcgcaag ggcagcgtca gctaatcaca attgcgcggg cattcttagc aaatccagat
    27181 gtcctgatcc ttgatgaagc aacatctagt gtagatacac gaacagaaat actgattcaa
    27241 aaagcgatga accgcctatt ggaaaaccgg acaagtttcg ttgttgctca ccgcttgtct
    27301 acaatacgcg atgcagacaa cattattgtg atgaatcatg gttcgatcgt agaaacagga
    27361 aaccacgata cattgatgga aaaagacgga ttctatgcag atctttataa cagtcagttt
    27421 tctgaagaag aagcttcata gaatagtaca aatttattat tttttttagg ggctgtgaca
    27481 aaagtcgtgt cacagtccct ttttccgaat aaacggtgtt caatcagctg tccttcggta
    27541 ttagctcaaa aaacggcaaa atatgagaag ctatttttcc gttttgtatc tctaatactc
    27601 aggtcaaacc gctgatttta caacctcttc cttaaacggt gttctcctgc caactcctcg
    27661 gtcacaagtc acaatagtca acaaacctga gaagctgttt tttgaaattg ctccttgtga
    27721 ctcggagtta aacggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagtttct
    27781 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac
    27841 tactcgaaac taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca
    27901 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
    27961 ttattacttg atgcagaaca gctttttcac aacctctttt tataagagtt tttactcatt
    28021 aatttacaaa aaacaagcaa aatgcgaatt ttctcacatt ttttttaaca aaatcaaagt
    28081 tattgcttga aagaagaaga atccatcgtt aaaataaagc ttgacgtaga ggggagcaag
    28141 aaatatgaaa gattttcaat tgggggctgc cacctattta aaaagtgtag ccattcgggt
    28201 gaaagaccga gataaaatga ttgattttta caagaacgtt gttggctttg atttgaaaag
    28261 agaagaaaac gaattagcca ttttaggaac agaagaagct ggaagtgaaa tgttgctgct
    28321 ggaagaaagt cctagagcga atgatcacat gggggtgatc aaaaagctga accgtttttc
    28381 attgattatc cctagcacag aagaaatggc agatatcctt tgtcgtatcc gtaaaatgaa
    28441 ttatccaatc aaagaagctt tagaagatca tggacgtatg gggattttgc tcgtagaccc
    28501 agaagagaat gaattggaaa tttaccatga agagaataag aacgaccaaa gagaaattcc
    28561 agaaacaatg gatcaagaag cattattaag aaaatctgaa gaaaaattca gtaaactatc
    28621 aacaggttct tattttgaaa aaatccattt aaatgttact aacttaacaa aagaacgcca
    28681 atttcttaat catgtacttg gtttgaaggt acataatgaa gaagagggca tccatgtatt
    28741 gaatgaagga gatttccatg ttggtttgac agaagctagc ggaggcgcaa ttgatttgcc
    28801 tacagatgaa gtgattggac ttgattttct gaaattcatg atcgatactg aaagcatgga
    28861 aggactgatt gctcacttgc agcaactatc agttgatttc ttcttagaca aaaagcacac
    28921 agtactcaca atctatgatg ctataggaat cgaatggtgg ttcgtcagtg agccaaaaga
    28981 aaagaataaa actgacattc gatgcgacgc ttaacttttt ctttcttaac gattgtcgca
    29041 tgtctcgtca ttttatgaac ggtcatacta tcgcttattc tagagataga aagagaaaat
    29101 agtataccgg gggaaactgc gcggtttttc gcgcagtttt tttgtgtaaa aaaggttgat
    29161 tcctcataga ttaagcttta agcttggact gtaagaatct gatagaaaag caggagaaaa
    29221 agatgtatcc acacacgaaa cagcttattt tgaaaaagat ggaagaagga ttttatccag
    29281 gtgtagcagt cgccttcata aaagaaaatc aagtagaaac atttacagcc ggctttgccc
    29341 aagtcgttcc tgaaaagcag ctgatgacag aagatctgtt gtttgatgca gcctcactga
    29401 cgaaagtcgt tgcaaccact tcattagttt tacgtctatt agaagatgga aagatagaac
    29461 tcgatcaacc gcttcataaa tatcttcctg aatttgaaaa tcagacaatc actttgcgtc
    29521 atttactgac acatacttct gatatcaaaa catggatacc aaacagagat aagctatctc
    29581 aggaagaatt gatcgccgct tatttgaagc tagaaccagg tgagcaacta ggaaaagaag
    29641 taaaatatac agatgccggt acgattcttc ttgggttgat gctagaggaa atttaccaag
    29701 aaactgcagt ggaaatcttt caagaacaag tattggaacc attaggaatg atgaacagct
    29761 tatttcttcc ttttccttcc aaaaaaatcg ttcccacaga acaactgcct tcgggtgagg
    29821 ttttgcgtgg acaaactcat gatccgaaag caagagtttt ggctgaacat gcaggtaacg
    29881 cgggtctctt tattgatttg aaagacagca ttacttttgt ccaaatgtat ttgaacaagg
    29941 gaaaaatccg taatggtcgt tttttagaag aagagacgat tgattcttta ctgcaagatc
    30001 aaacaccgaa aaaaaatggc tatcgttctt taggatggga cttaaaaaag gatgtagaag
    30061 acggtcatgc gattcttttt catactggtt atacaggtac gtttttcttg attgatataa
    30121 aaacacaaga agcgttgata tttttgtcaa atcgcgtaca tcctgtcgat catcgtgcgg
    30181 agtatctcat atgtcgagat gaaattgttg aaaacttttt gaaagagaaa gcggtattac
    30241 cgaaataatg ctataatagc tatgaaaaat gagaggagtt tgaattatgc aggaacctat
    30301 gtttttaaaa ccagtttttc aagaaaaaat ttggggagga agtcgtttac gttcagtctt
    30361 tggttttgat atccctaacg ataaaatcgg agaagattgg gcgatcagtg cgcatcctca
    30421 tggtgtcagt gtagtagaaa atggcgaatt caaagggaaa agattagatg aactatggtc
    30481 agaacacaag gaattgtttg gccatccttc agaaccagtg tttccattat tgatcaagat
    30541 cttagacgca gaagatgatc tatctgtaca agtacatcca gatgatgcct atgggatgaa
    30601 acatgaaggt gaattaggaa aaactgaatg ctggtatatc atcgatgctg aacctggtgc
    30661 agaaatcatc tatggtcatc atgcaaagac aagagaagaa ctagctgaga tgatcaaaga
    30721 tgggcgctgg gatgaccttt tgaagaaagt accagttaaa aaaggtgatt tcttttatgt
    30781 accaagcgga acgatccatg ctattggtaa aggaatcatg attttagaaa cacagcaaag
    30841 ttctgacacg acttaccgcg tgtatgacta cgacagaaaa gatgcgcaag gacaaacaag
    30901 agagctgcat atccaacaat cgatcgatgt aacaactgtc cctgcgaaaa caccagaatt
    30961 acaaatcaaa gaagtccgaa aagggaattc atcgatcgtc acttatttag aaactgagtt
    31021 tttcaatgta tatgagtggg atattaaagg aatcacatct ttcaaaaaac aagcccctta
    31081 tacattgatg acggtaatcg aaggagcagg agaccttgtc gttgacggaa aaacatatcc
    31141 attagaaaaa ggaacaagct gtattattcc aaatggcgtt tctgagtggg cggtccaagg
    31201 tgaactagca atcattgcgt ctgaaccagg caaatagttc ccagatagct gaaagcagca
    31261 gacggttttt cgtttgctgc tttttctaca taaaatccgt ccatcaataa atagaagtct
    31321 ggtgaaaaag atggaataca aatggaaagc aaacaacagc agtcgcgtgt ctatgaaaca
    31381 actgagaaaa gctggacatc acttaaatga aatgatcatg ccaaatctaa gtctgtttat
    31441 cgcttgggga atcttgtcat tagcaacaaa aggtgtgtct ggcgatttgc acaccacatt
    31501 agaagaggta tgccattgga tgatccagct gcttttaccc atcttgatca gttattcagg
    31561 cgggaaatcg gtagggggac aaaaaggggc agtcgccggt gcaattgctt ctttagggtt
    31621 gatcgctcgt tccaatgctc cacaaatcgt aggagcgatg attatcgggc cgcttgccgg
    31681 catcgtatat gaacaatttg tttctagatt ccatgtgaaa ttcaaggctg gatatgaaat
    31741 gcttgtcagc aacctactag taggactgac tggtagcgtt atttgtataa ttggaatcgt
    31801 ttttattgaa ccagttttaa atagtgttca cttgttgctc gcctccgttg tgtcttggct
    31861 agtcacaatg aatctgttac cacttgtcag tctgatctta gaacctctaa aagtactatt
    31921 tttcaataat gcaatcaatc atggtgtatt gacaccttta ggaatcgaat tctcacaaac
    31981 agtcgggtac tcttccttat ttttgatgga ggcaaatcct ggacctggtt tagggatctt
    32041 actttctatc ctttgttttg cagaaaaaca ggaaaaagta aatgcgagtg gagcattgat
    32101 gatccatgcg attggcggga ttcacgaaat ctattttcca tttgtcttgc tgcgaccgta
    32161 tctattttta gctgtaatgg ctggtggggc aagcggcacg ttgatatttc aatggttaga
    32221 tacgggatta gctgcacctg tatcgcctgg gtcactactc gtgatttttg ccaatgcgcc
    32281 ttatccacaa ctagctgggg taagtatagg gatagcaatc agtacatttg tgaccttcat
    32341 ttgtgcagcg atactattga aattaccaaa taaagaaaaa gagaacaaaa cggtcaaaga
    32401 aaggggagaa atcttggaac catctatcat tcattccgtc atttttgctt gcgactcagg
    32461 tatgggatca agtgctatgg gagccgccct gctgaaaaag aaattagcag cagaaaaaat
    32521 cgatctttct gtcggataca gctctatcta tgaattgaat aaccaagtaa atcagctggt
    32581 tatcgtgcaa catgaactag cgggtcttgc aagagaaaaa gtacctcaaa gtcaaatcta
    32641 tgtactggat cactttttgg aaacagatat cttgctgtca cgaataaaag aagcgatcca
    32701 atttccaaag cgggaagaaa aaaaagagat agcagatatc acagaacaaa aaacgtcgcc
    32761 tgctaatatt gtatttttgt ataaaaacaa cagacgcggc tcacaaacta tgggcatgac
    32821 gattttaagt caattagcaa aaaaagcgaa aaaacagttt gttatctcaa aagaatcaat
    32881 agaaaaaatc aaaatccaag aagaaacgat ttatatcgca caaagagaac tagctgtaac
    32941 tgaacagcta gaagaaaaga tccctcggct agttctagtt gaacattggg tagctacgaa
    33001 tgaatacgaa aaactcgtga agggagaaat ataagatgta tttatcgccc agagaaaaac
    33061 aattattagc agaatttctc agtagcccgt cacctgtatc tatccaaaaa atgatgaatt
    33121 tattgaaggt cagtaaacgg actatttacc gcgaattaga taatcttgaa gtctcattga
    33181 aatcggtaaa tgcttctttg aaaaaagcag ctagaggctc atttgtcatt gaggcatcag
    33241 aagaagtatt agagcaatta aaagaaaata cagatgggaa aagtaatgac ttatccactg
    33301 ctaaacgaca acacgcgatt ttagccgagt tgttactgac gacagagccg ttgagtcttg
    33361 ctcatttcat ggaatcatat cgcatcagta ataccacttt ttatgcagat atcaaacagt
    33421 tggaagaaag tattcgccaa ctgcctttgg aaatcattcg gaaccaggga tacgagatcg
    33481 aaggaccgga aaaataccgc cggcttctga cagcgaatgt attagaatta gaaatcaatg
    33541 aatatgaact ttttcatagt atttctttcg atgcatcact gaattatttt tttcgattcg
    33601 ttgatccgca acatctatct cttgcacgca aagtaatcgg ggaagaattg atccaaaaaa
    33661 agaccaatct aagtgataga aagcttgaac atttggtttt gatgctgaca ttgacgatgg
    33721 agcgtgtgac gaaaaatcat ttactgacaa atgagacata taatagtctg gcaaacaaag
    33781 aattactaca gacagctaaa cgattgtttt cacggatcgc ttctgagaca aaacaacttt
    33841 atccagtaaa tgagatcgtc ttttttgcag gattattaaa tgacttcacg aactcatttg
    33901 atcagaattt ctttgaagag acgttcgata cacagcttgc ttatttagta aaacaactga
    33961 ttcaagaagt aagtgaagag acgaatgtcc atttttatga agatgaaact ttgtacaaaa
    34021 tgctgttgac tcatttatct ggtgtgtttt caagagccgt tttacaagaa gagacactga
    34081 ccaatccgat tttagaaaaa atcatggttc aatacgagga agttgcgaat gcaatccgta
    34141 aagcagtaga gcggattttt caagaaaaaa aattatccga agaagagatc gcttatatgg
    34201 tccttcattt tgcgaattca ttggaacgaa gtccgaaaga aagcaatatc agtattgcag
    34261 gattttcgcc aagtggatta gcttctacta gcatgttgga actacgattg aaaaggtatt
    34321 ttcccttcat ccaacaaatt catttctatc ggattgctga tttaaaaaaa atcgatttac
    34381 aagaagaata tgatgttgtt atttctacat ctattttacc aggatacggt ggaaaatata
    34441 ttttagtctc cccgctactg ctagaagatg aagtcaagca gctaaaagaa gctttttcgc
    34501 atattgagaa gaaaaaaagg caaaaaatta cgtataacag caataacgag tggatgatag
    34561 aagatactta tgaggaaaca atgcatttca tggatcaaat caatcaatta ctcggtcgtt
    34621 tttttatcca agaactggat aatcctgaga cgatttccga aacgattcgc agcttatttc
    34681 cttattttcc tgaaggacta gtaacggatg aagaggtggt tcatcgccgg ctgcttaagc
    34741 gatatgaaca agcacctatc ggtattcctc atacaaatat ggggttattc catacatcca
    34801 gctcattgat ttctaaaccg atcttttgta ttttcaattt gaaacatccg ttgaccattg
    34861 aagggatgga tagacagcca gtcgatttgt cccgtatgtt agttatgctg gcgccttcgc
    34921 caatccaaga aaaagacggg attcttttag gaaagatcag tggagcaatc atcatgaatg
    34981 acttgaacac cgagatcttc cgctcaggaa ataaagaaat cgtttatcag ttgttagcga
    35041 aaatcctgat tgaagaaata aagaaatgaa ggtgaaaaga tgatacagct accagcagaa
    35101 aaaatattgc tcgatcaatc ctatcaaaat tggaaagaag cgttgaccga gatcggccag
    35161 tggatgaccc aacaaggggg aatcaaaccg gcatatatcc aatcaatgat cgaaagacaa
    35221 gaaaaagcga gtgtctatat cgggaatttc gtagccttgc cacatgggac tgacaaagat
    35281 gaagaagtag tagaagaagg aatctattta gtccaagtgc ctgatggtgt ggactttgga
    35341 acaaaagagg aaccaaaaat cgcgacactc ttatttgtag tggctttaaa aaaagaacat
    35401 cagttagcgg tgctccaaga tttagccttt ttctgttcag acatcgacca agtaatggcg
    35461 ctcagtgatg cggaaaccat tgaagaagtc caaatgattt ttgaacatgg acatatataa
    35521 ccaatgttca cagtaaaaaa agctgccttg catcaagtaa taacttcaac caagataaaa
    35581 ataagaggag ctatttttgt tgggaggttc ttattactgc ggatgcaagc ttttgaaaac
    35641 tatttattct agaaaaccgt ttatttgaaa taggggacgg tgacaaggtt tttgtcaccg
    35701 cccctattat ttttgtgttt taatagctct tttttatata gatagtgccc atacctatga
    35761 ataataaaac gatactccat ataccaagtg tcaagatacc attcatgaaa gtaacctgtt
    35821 ctgaagctag tggcgagaaa atatctcctc ctatgatcac attttgaaaa tcaacataag
    35881 aagttggcaa gtaatgactg attgattcta tccatccaat cgaagaaaac aagcgatact
    35941 ctgctaagaa aagacaaaga agtgtaccta aaagattgac tagagaatgt tcaaataaag
    36001 cagatagaag aaaactgatt gtacttaaaa agagcaccca caacaacagg aacagaaagc
    36061 tctggaaaag atactcatac atcggcagga ttcgtatctc ctgaccaatg atcgttccta
    36121 caggataacg gaagtctccc aaaccatttt tgattattac tagtatagag acgatcaaaa
    36181 atggcatcac gagacttagc atagctgcac cataataaat ggtaaaacgg atgctatgtt
    36241 tctttaccat actatccggt aatagattga ctagagagat tacctgcttt cttttttcat
    36301 aagagatgat ggaagaaaga aacaaggcgg ttatacataa aatcaaaaaa ctagaaatca
    36361 ttcctgaaaa aatattttcg tagtaatttg ctaaagagag atgcgctggc aagtcaaaaa
    36421 tagaatatct ttggatctga tgttctttca tataacggag caactcgaca cgtttttgtt
    36481 gttcaatgat tggaattcct tgtctttgac cagaaatcag tcgatccagt atatcttttt
    36541 cataatgata agttgcttcg accatttgct ggacattccc tgtgtttttt gcatgtaaga
    36601 ttgcttcgat cagtttgttg cttttctcta aactttccac aaattctttc tcattctcat
    36661 cttctttcaa cgtttcaatc aaacgttgca tctcttcata gttatgattc agttcatcga
    36721 attctgtata agcaagttta tgatcaaaaa aaagtgggaa tgcaaagagc acgaccaaca
    36781 atagaggaaa taaaaagttt actttgtttt ttagtaagag attgcgttca aatgtaagta
    36841 aagacatagt tacacctcat ataatttacg ataaatagct aaaatctctt ccatatttga
    36901 ggattctgtg acgatttctc cattttttag aaaacatgct ttagaacaaa tcgattggat
    36961 attcgcgata gcgtgggaag aaagaagaat cgtcttcccc tctttctgcc attgtctgat
    37021 gaggctattc aagatatcaa tacttcctgg gtctaatccg ttcaacggtt catcgaataa
    37081 catgacagat gaatcactga tcaagtaagc agcaagcaag acatgttgtt tcattcctag
    37141 agacattttt ccaatcggca aagtaaggta ctcagtcatc ttcaatcgtt ggaaaacagt
    37201 ttggcattct actttgctct gccaatagta tttgatcaaa gtcaaataat caacagccga
    37261 tagatagtcg gataactgat tcgttgattc tagaaaaaag aaagaccggc gataatctgt
    37321 ataagcagta ggagagatgt tgtgaaggga caattgatca taagttgttg gcagcagttg
    37381 ggcaatgcca ttcagcaaag ttgttttgcc aaatccgttc ggggcaatca aagcagctac
    37441 ttctcctgat tctactaaaa gattcaattg tcgaatcaca cgttttttac cacgtttcat
    37501 gttcatgttt ttgatcagta atgtcataga taggcctcca atataaaatt taataacata
    37561 atagaatgga aaacatctaa attaaatgct aacaaaagtt attgtttgca tattataaaa
    37621 aaattaaaat aacagtttta tttaaaattg aagttttctg ataccgaaat ctttttattt
    37681 tgctgacata ctcaaacctt tggcacaaag tttcgtgcca aagtacgact atttctcttt
    37741 ttagtaagcg gatacaatta gagtatcaaa aggagagagg gagagacaaa atggaacgga
    37801 cgattcccaa aaacaacatt tcgctaaaag cgagggtaca aaaattaggc agtactttat
    37861 caagcatggt catgccaaat attggagcac tgattgcctg gggtgtatta acggctctat
    37921 ttatcccaga tggttattta ccaaatgagg ctttcgctac catggtaagc ccaatgctaa
    37981 cttatctgat tcctttattg atcggttata ctggcggaaa agtaattgct ggtgaccgcg
    38041 gatcagttgt tggggcaatt gcaacgatgg gtgtgattgt cggaacagat attccaatga
    38101 tgctaggcgc gatgatcatg ggacctcttg gtggatatgc catcaagaaa tttgatcagc
    38161 tattccaaaa acggatcaaa tccggttttg aaatgctagt caataatttt tcagcaggtc
    38221 ttatcggttt tggattagcg ttattaggtt tttctgcgat tggtccagtt gtcgatgcat
    38281 tgactcaagc aatggcaaaa ggtgtagaga tcattttatc tgcccatttg attccattga
    38341 caagtatttt tattgaacca gcaaaaatct tatttttgaa taatgcaatc aatcatggga
    38401 ttttgacacc attgggaact gaacaagtag cagaggctgg aaaatctatt ctattcttgc
    38461 ttgaagcaaa tcctggtcca ggacttggcg tattacttgc ttttactttg tttggcaaag
    38521 gagcagcaaa atcttcagca ccaggagcca tgatcattca ctttttaggc gggatccatg
    38581 aaatctattt tccttacgtt atgatgaaac cgttattatt cttatcagtt ataggcggag
    38641 gaatgtcagg aagtttcatt ttccaattga tggatgctgg actaagagcg ccagcttctc
    38701 cagggtcgat cattgcgatt ctagcgatga caccgttcaa ttcttacctt cctgttattt
    38761 tgggcgtgat tgttgccgca gctgtttcgt ttggtatttc tgcagtaatt ttgaaagcgg
    38821 atgcgaaaga tacaaatgaa gacttcgaaa aatctgttca agagacaaag gcagctaagc
    38881 aagcttccaa aggtgcccaa ggtattcctc aaggagctac attctctggc gtacaaaaaa
    38941 tcatttttgc ttgtgatgca ggaatgggat caagtgcgat gggcgcttcg atcttgagaa
    39001 aaaaagtaca ggaagcagac ttgcctcaaa cggtgaccaa tcaagcaatc agccaactga
    39061 cagatgactc gaatacattg atcgttactc aggtcgaact acaagaaaga gcaaaacaaa
    39121 aagcaccgag cgcgcgtttt gtctcagtag aaaacttttt gaattcacct cgatatgatg
    39181 aaatcattca agaattaagc agcaagcaga aaacatcagt ttctgaaatt gagaatgagc
    39241 cagcagaaga gtttctttca gcaaacatca aacaaatcaa agagattcta cttgtccacg
    39301 atgatcgttc cggatcagca acaatgggga tgaccgtgct caaagatata ttagacaaga
    39361 accatttaac tatcccgtta agaaaagtca atatccaaga tttaaaagat gaacctgatt
    39421 cactgatcgt agccaaagaa agtttagcgg aaaaagtaca gatggctgca cctcaatcca
    39481 ttcatttatc agtcaacagc atggtcaatc cgcaaaaata cgaatcgatt gcacaatatt
    39541 tgaaacagac agcttaaggg aggaaaacag catgcaaagt ttgccattcg acatgatcga
    39601 gctcaatcga tcttttaaaa ccaaagaaga agcgatccgc tattgcggtc agatgcttgt
    39661 agaagcaggg tgcgttgatg aggaatatat cgatgccatg gtagatagag acaaaatgtt
    39721 atctgtttat atggggaatt ttatcgcgat tcctcatgga acagatgaag caaaaaaata
    39781 tgtgaaaaaa tccggcatct gtgtcgttca agtaccagat ggtgtagatt ttggtaccga
    39841 agaagaagaa aagattgcga ctgttttgtt cggtattgca ggagtcggag atgagcattt
    39901 gcaacttgta cagcaaatcg cactttattg cagtgatatg gacaatgttg tccagctagc
    39961 agacgcgctc agcaaagaag aagtaacgga aaatctagca attgcttaag gagagagaaa
    40021 aatgagagca gtacattttg gtgcaggaaa tatcggtcga ggatttattg gagaggtttt
    40081 ggcagctaat ggatttgaga ttactttcgt agatgtgaat cacacgatca ttgatgcttt
    40141 aaatgaaaga aaagaatacg aaatcgaatt agcagatgaa tcaaaagaaa aaatcactgt
    40201 aaaaaatgtc aaaggaatca acaatcaaac aaatccagaa aaagtaatcc aagaaatcgc
    40261 tgaagcagat ttagttacta ccgccattgg cccgaatatc ttacctttta ttgctgagtt
    40321 gattgcaaaa ggtatccaag aaagagaaaa ggaaggaagc acaacatctt tagatatcat
    40381 tgcctgtgaa aatatgattg gcggttcagc atttctagaa aaagaagtgt ataaatatct
    40441 tccggaaaca tcatttactg acaaatatat cggctttcca aatgcagcag tcgatcgaat
    40501 cgtcccatta caacaccatg aagatccttt attcgtccaa gtggaacctt tcaaagaatg
    40561 ggtcattgac gatagccaaa gaaaaaataa aaaaatccaa ttaaaagggg ttttatatgt
    40621 agatgatcta gaaccatata tcgaacgcaa actatttagt gtcaatactg gtcatgcgac
    40681 tgtcgcttat acaggtgcct tgttaggtta ccagaccatt gatgaagcaa tgcaagatgc
    40741 gttagttgtg gcacaattaa aatcagtttt gcaggaaaca ggaagtttgc tgattgccaa
    40801 atgggggttt gatgcagagc agcatcatgc gtatattgaa aaaatcattc atcgcttcca
    40861 aaacaaaaat atctctgatg cgatcacacg tgtcgctcgg acaccattac gtaagttagg
    40921 ttatcaagaa agatttacac gtccagtgag agaactacaa gaacataact tgacttgccc
    40981 acatttgacg gcaacaatgg ggattatttt taattactat gatcctgaag atgaacaaag
    41041 ccgtcaattg catgaaatga agatccatga aaacttggaa caattgattc aagaagtcac
    41101 aggaatcaat gatccgaaaa caatcgggaa tatcaagcaa aacgtgaacc gttatgccaa
    41161 gcaagtcgct taaaatagga tacgaaaaag gggaatgtga cgaaattttt gtcacattcc
    41221 cctttttcga ataactaggc tgtttgtcct tcaagatagg gcagatccaa ttctttaccg
    41281 taacgttgtt ctaaagctag gcggatcaaa cgagctgcca ggttctcatt tcttgcaagg
    41341 ataggtccat ggaaataaga accgaagaca tttcggtaaa tgacaccttc agattggtct
    41401 tcaccattat ttccttgacc ttgtaccact ttccccaaag gtcgttcacc ttcacctaag
    41461 aaagtccgac cattgtggtt ttcaaatcca taataagttt cattgaattc ttcgttatgg
    41521 ataacgatat caccgataaa ccggttattt tcctgactta acgtgtagtg atccagtgca
    41581 ctgattcctt ggatcttttc tccattagct cccatgtagt agtgacctag caattggtag
    41641 ccaccacaaa tggctaacat gacgccatca ttttcgatat agttgatcaa agaatctttc
    41701 tttttctgga tgtcttttga aatgattaat tgctcaaagt cttgaccacc gccaaagaaa
    41761 accaagtcat atttatcagc atcaaaaggt tcatgtatac taacgatctc cgtatggcaa
    41821 gaaaccccca ttttttctgc tatatatttc aacatcaaaa ggtttccgtt atcaccatat
    41881 gtgttcatca aatttccata aaggtgggcg atattgagtt cataattagc cacggttcat
    41941 tcctcctttg ataaatcctt gagcagctag ttctttacga agctgcaata cagcagtgta
    42001 agtagctaaa atatagacat gctcagttgg caattctttg attttttcga ttacttctgg
    42061 taattctttt gtttctgtca atttattttc tggaatcccg gcaactttta gacgtaaagt
    42121 catatctgta tgacggtctc ctccagaaat aacagctggg atatccatat cggcaaagtt
    42181 ttcaaaatta ccatcccaaa tccagctgac atcgattccg tctgcgtaat tggcatttaa
    42241 taaagccact aaagaaaatg gataaggagc tagaccaatc atatcgatta cttgattgat
    42301 acctacagga tttttgacaa gtactaatgt acattttttc ccattgatat tgatgacttc
    42361 ttgtcggccg aatactttct cgtcatatcc taaaccttgt ttgattttat caggatcgac
    42421 attgaagtac tctgccactg cagtagctgc taaagcattg tagacattat acataccgcc
    42481 aacttcgatt ccgtattcat gaccatcgat cacgaaatct gctgacacat tgtccatccg
    42541 taccatatca gttaatcgat aatccaattc aggacgtttg aagtcacaat ttggacagta
    42601 atatttccct aagtttgcat aagtgatcat tttataatgc aagatgtgat gacaatttgg
    42661 acataagaca ccgtctgtat tgtaatgagc catttgttca cgatcttctt catggtcaaa
    42721 gccataatat tttctaggat tgactgtttc gatagaattg aagattggtg aatcaccgtt
    42781 actgataatc gttgcatttg gtgactttgc ggcaccgtct acaatcattt tataggtcgt
    42841 atagatttct ccataccgat ccatttgatc acggaaaata tttgtaaata agaacagttc
    42901 tggtttcata tattctgtga ctttgctcaa acttgcttca tctatttcta ggacagcaaa
    42961 ctttttctgt cctttttttg gttttgcctg caagaaagtc gatacaattc cctgtaccat
    43021 attcgcacca gtagggttgg ttagaacttc atcgaattgc tggcgcaaaa tattgactgt
    43081 taaagcggta gtcaatgttt taccgtttgt tccggtgaca acgacgactt ggtagtcttt
    43141 tgccaaacta tctagtatat gtggatcgac tttcagcgct agtttacctg gtaaacttga
    43201 tccgccttta aaaaaagttt ttaatgccca ttgcgacgtt tttccaactg caatcgcaaa
    43261 atgactgcgt attcccatta tgtaacctcc aaaaacgatt tgctgacgaa taaattcaac
    43321 taatcatacc atatgttgaa aaaaaggtta aattgctttt cttattctta agaaaaggtt
    43381 acggctgaaa gtgcatgaaa tcggacaaaa tcgcatgatt taacaaaaaa acagcttgaa
    43441 acaaaatgat tgtttcaagc tgctgtgatt ttattattcc ctcttaccta tattcccatt
    43501 ttaaccgata ataattttct cgcttggata ttcatagcct agatctcgtg tttcttttgg
    43561 cacgaataac atcaatgtat atagcattcc gatccgccca ataaacatca gtgtagcaat
    43621 cgtgattttg cccacaacag acaagtctcc ggtaatccct aaagataaac cagttgttcc
    43681 aaacgcagaa gttacttcga tgatgatcga gatcagcgtt tgttcttctg tagcagataa
    43741 aaaaacgata caaaagaaac acataccaag agaaagcata aagacaacga ctgattttcg
    43801 cacatcatct tgatcgattc tccgtccaaa aatattgatg ttatcttcac ttttgaggaa
    43861 ggaatataga tacaaaccga tgattgctac agtagttgtt cgaatcccgc cgcctacaga
    43921 actaggactg catccgataa acatcagtaa agaaaaaatg atcagtgttg tgatttgaaa
    43981 atcacccaaa tcgtgaatct gcagaccagc attacgagtc gtaatcgaat aaaacatcga
    44041 attgatccat ttgacactca tattggaatc ttggaataaa tggtcctttt ccaatagata
    44101 aatcaagaca gttcccgaaa caaacaagat aacaaaagct aacacagcta atttggtaaa
    44161 caaggaaaaa cgaaacggaa gcttcgcatt ggaacgtttg tataatagcc attcccggca
    44221 ctccatcaat acaggaaaac caattcctcc gacaaaaatc aaaaacatga tcaggatcaa
    44281 gaaaaaatag tcatgtgcaa agggcttgat ggaatcccca gtgacgtcaa acccagagtt
    44341 cgtgacagcc gatatcgcct gataaaaacc gtaaaaaacg gcatcacgcc aacgatcaaa
    44401 atagcctctg taataaaaat aaatagaaaa gaatgtccca aaaagtaatt gaaaccagat
    44461 taaaatagca aaagtgatcc gaatcagtcg gacgatacca gacaaacgcg gttgattcat
    44521 atccgtcatg atcagctgtc gctgtttcag tgtgatccgt cgtttagaaa aaatgacaaa
    44581 agcagtagaa atcatcatga tccccaaacc accaacttga aataatattt ccagcagtat
    44641 aataccgtta tcattgaaca cagaattgat atcaaaagtc gatagtccag tcacactcac
    44701 tgtactgatt gccatgaaca gcatgtccac aaaagaaaca tggctatctg gttcgcgaaa
    44761 aatcggcata taaaatagaa cgagtgataa gaccgtcatc aatatataat aaaaaacgat
    44821 gatttggatc gatgagaaat gattggcagc aaacttctgt cctcgccgct gcagccttcg
    44881 aaagaataaa tcaacatgca cagtactatc ctcctgacaa tagtcgtttc taccagtata
    44941 caaggggaat taggaatact caaagaaatt atcaaacaga taacaataca gctttcgatt
    45001 aaaatagaaa tggcttataa taaggaaaat cctagaaaaa agaaggagga ggaataacaa
    45061 attgattaat gctcaacaaa tcgtagaaaa aatgaaaaat caaaaaataa attatgatac
    45121 agttctgaaa aaaatgatca tgcaatggga gaaagaagaa gtaaagccta agatattaat
    45181 acatacatgc tgtgctcctt gcagtacgca cacattggaa ttcatgtctc aatatgcaga
    45241 gatcacgtta tttttttcaa attcgaatat tcatcctaaa agtgagtatt tgcgaagatt
    45301 acatgaacaa aaacgatttg tagaccaatt taatgagaaa actgaccatc atgttgcgct
    45361 aatcgttgat gaataccgtc caaatgaatt caccaaaatg gttttaacaa accatcttga
    45421 aaacgaaaaa gaaggtggtg ctcgctgcag tgcatgcttc aatatgcgat tagatcttgt
    45481 tgcgcaaaaa gcgcaagaac taggatacga ttactttgga agtgccctga ccatctcgcc
    45541 aaagaaaaat gcagctttga tcaatcagat cggaatggat atccaaaaga tttacgggac
    45601 acaatacttg ccaagtgatt tcaagaaaaa caaaggctat gaacgctctt tgcaaatgtg
    45661 caaagagtat gatatttatc gccaatgtta ttgtggttgc gtgttcgctg ccaagcagca
    45721 aggatgcgat ctgaaagaaa tcaatcggga agcaaaagct tacttaaaaa catcaaccgt
    45781 agtttttgag gaaaattaaa gaaatctttc ggaaaaacgt aagaaaaaaa taaagaattt
    45841 atggtagaaa taaggtagac tgtataattt gtccgtagta aagtaaggaa acaagaataa
    45901 atttcaatga aggaagagat aagatgcgaa aagtagcacc tattttgaag ccaatcgctg
    45961 tattaacaat ctcgacgatt gtcgcgacta ctacaggtat tgtgaatagt tccagctacc
    46021 gtgcaccaag ttcaagttct agtactgtag aagaaacaac accaactact tcagtctcga
    46081 catctgaacc aacaacaacg gaaagtttga atgaaaccac tacgacaagt acatgggacg
    46141 atacaacaac tagttcgtcg tatgatgaga catatacaac tgacagctca agcgaaacaa
    46201 gtaatacggt accagatggc actccatcgg attcaaccaa tccaagtaca agtccggata
    46261 caagtgctag ctccgaaacg ataccaagca gtacgactgt agatgataac acgcaaaatg
    46321 atgatgcagc acgtacagca cagcaaaacc aagattcagg aaatacccaa acgtataacc
    46381 agcaagcagg tgattctcaa tgacattttt actagacctt atcaacgggg tgcataaatt
    46441 ccttggctat ttagacatca gtcctaaata tcttaaccgt ggatatacga ttttaagtgt
    46501 gattcccacg ctttatttat tgagaattgt ttatggactt tggcaaaacc aaaactatct
    46561 tcaatttttc ttatatggac ttgcatttct agtgcttgtt tattttactg ttttaaatgt
    46621 attttattac tttttagata aaaacacgaa agccgatgtc actcagcttt ttgtgaagta
    46681 tttacctgat gaagcgttca acatccagac agaagaatcg atagataatg gaaattcgat
    46741 cgataaagtg aatacacaag aggtcatggt ttcttatgac gaagattatc aattgaaatt
    46801 agccgagaat atgcggtatt tgattggaaa tggtgagatc aaaacaaatg aactcggtcg
    46861 catggatgga tttctggtcg atagaaatac attgtatcct tattactatg tcaagaaaac
    46921 aggagagaaa gagtatgttc taaatattgg acgaagctac tcggacatgg aaaaagtagg
    46981 gaccattcga cttgactcaa cagaagacac attggaacct gtcggattgt tcatcgtcgg
    47041 cggtgatttt gtcaaagacg ggttacgcta tcatgagcct tatcgtctga aattgattgc
    47101 aaaaaaggca cagtcgcagg aagaactagt ggtttatagt cgtagccaaa gaagaagaca
    47161 ttcataatgt gatgaaacgc cgtgtatcag tggttgcgat aaaaacgttt attcggaata
    47221 agggggatgt gacacgtctt ttgtcacgtt ccttttttat ataacgagaa agataagacg
    47281 aacacatcac ttattctttc ctttcattta taaacattgt agaataaaga aagtacaacg
    47341 aagtattgtg aaaaaacgta ataaataacg aggaaaatac aagtaggagg taagctgtga
    47401 aagaattttt gaaaaattgg gggattttga tccttgtatt agcggctatt ttattagcta
    47461 gagtgtacgt gtttacacca gttactgtaa acgggcattc gatggaccct acattaagtg
    47521 atggacaacg attgatttca tctaagatct caaactatga acgaatggat attattacta
    47581 caaaggaacc gggggatgaa gaacggatga ttgttaaacg gatcatcggt atgcctggag
    47641 atacagtcaa gatggaaaat gaccagttga ccattaatgg caaaaagtat gatgaacctt
    47701 atctggatga gttcaaaaaa gaattttcag aagataagtt acaaggagaa tatgcttata
    47761 gctcaggatt ccaagcacaa gcagaaagtt catccacctt tacgagtgac tttgaatata
    47821 ctgtgccaaa aggaaagtat cttgttctag gcgataaccg cttgatttca aaagacagcc
    47881 gaatgtttgg gttagtcgac aaagatatga tccaaggaaa agttgttttc cgttattggc
    47941 ctctttcaga aattaaaatc atgtgatgaa aaaataaaaa gaagctgtga catgtttttt
    48001 gtcacagctt ctttttccga aaattccttc ttatttcttg gagttgctca ctttttttgc
    48061 aatctcattt taagcttttt ggctgatgga taagaaaagc gaagcgtttt tgttcataaa
    48121 tagcttttcc ccacgatgga aggtatgact agtagcagag acaaagcccg ttttttccaa
    48181 tcgattattc aattcttctt ggctaaagcc attatggact ttcggatgac taatttgttc
    48241 attcttgtca aagtctacga tgatcaattg accgccagga gctaaaattt cataaagttt
    48301 tgtaagaata ttctccgtat caggaatgtg taaaagtaca agtgatagaa ggatgagatt
    48361 agcttggaca gcaggaaatt cgacagaagc atcagcgtgg atcgttcgga catttttcag
    48421 atctgctgct tggatctttt cttctgccat tttcagcata gtttctgaag catcggcaat
    48481 gatcagttct ttgaatcttt ccgcaagcgg taaactaaca agcccagttc cgcctccgta
    48541 atccagcagg actttttggt ggctgtcgcg cggtagataa gtagttagtt cttcgcgaat
    48601 aatcttggct aatgcttgac ggtcagggga atcataatgg ttagcgatgg aatcaaaaat
    48661 attttttgtc atcaaatagc tccttacgta aaactttgaa attttaactt attatagcac
    48721 gtgaaaagaa agaaagcatg ctatactcaa ttcaaaaagg gagaatctag aaagaatatg
    48781 ccgatatcgt ttgtaaaaga tagagaggaa aaaggaaagt gtgtccgaga aatattacta
    48841 gacttgcctg agtggtttgg gcttcctgaa tcaacggaaa aatatatcga ggaatcaagt
    48901 aaactacctt tatggtgtga aaaaagaaaa gaagaatatt taggctttat cactctttca
    48961 cagacaagtg aagatactgc cgaaatctat tctattgtat gggaataaaa aaagactatc
    49021 atcaccaagg attaggaaca aaacttttta aagaagcaga aggttacgca gcaaaacatt
    49081 acaaatattt gcaagtgaag actgtagacg aggggcacta ctctatatat gatcaaacta
    49141 tttgtttcta cgaatcatta ggattttcaa ggttggaagt ttttccaaat ttatgggatg
    49201 aatggaaccc atgtttagtt cttgtcaaaa aattggaaca aaagtgagca atagatttaa
    49261 tactaatctg tgcttgatga aaattctcac tgtaaaaaat gctgaaaatc aagtataata
    49321 ccataggaac aaactggaag gtgaaaaaga gcatggcaca actatttttt aagtatggcg
    49381 caatgaatag cggcaagacg atcgagatcc taaaagtcgc acataattat gaagaacaaa
    49441 ataagccagt tgtcttaatg acaagcggta ttgatacaag agatggttta ggcgttgttt
    49501 caagccgaat cggactgaaa cgtaatgcga tgccgatatt tgaagaaaca gacgtatttg
    49561 acgtaattca gaaaatggaa agcaaaccct attgtgtgct gatcgatgaa gcgcaattct
    49621 taaaaaagaa acatgtcatt gcttttacga gaattgtgga cgaattggat attccggtta
    49681 tggcatttgg tttgaaaaat gattttcgaa acgagttatt tgaaggatca aaatatttat
    49741 tgctttatgc agacaaaatc gaagaaatga aaacaatctg ttggttctgc cacaaaaaag
    49801 caatcatgaa tttacattat attgatggaa accctgttta tgaaggcgat caagtgcaaa
    49861 ttggcggaaa tgaagcttat taccccgttt gtcgaaaaca ttactttcac ccatcaatga
    49921 ttacagaagg agaataagca tgtacgatca attacaagca attgaagacc gttatgaaga
    49981 gcttggagaa cttttaagtg acccagaagt gatttctgat acaaagcgtt ttatgcagct
    50041 ttcaaaagaa gaagcaaaca cgcgcgaaac agtcgaagtt tatcgtcgtt ataaagaagt
    50101 agtacaagga atcaccgata cagaagaact gcttggtgaa aaactggatg atgaaatggc
    50161 tgaactagca aaagaagaat tatctgaatt aaaaaaagaa aaagaagtct tagaagaaaa
    50221 aatcaagatc ttgcttttac caaaagatcc aaatgacgat aaaaatatca tcatggaaat
    50281 ccgtggagca gcaggcggag acgaagcagc attattcgct ggcgatcttt tcaatatgta
    50341 ccaaaagtat gctgaagcac aaggctggaa aacagaagtg atggaagcta acgttaccgg
    50401 aatcggtgga tataaagaag tgatcatgat gatcaccgga gacaacgtat attcgaaatt
    50461 aaaatatgaa agtggtgcgc accgcgtcca gcgtgttcct tcaactgaat cgcaagggcg
    50521 tatccatact tccaccgcaa cagttgtcgt gatgccagaa gctgaagaag tagagatcga
    50581 tattgaagac aaagatatcc gtacagatat ctatcatgct tcaggtgctg gcggacagca
    50641 cgtcaataaa acagcatctg ctgtacgttt gactcactta ccaacaggga tcgttgttgc
    50701 tatgcaagat gaacgttctc aaatcaagaa ccgtgaaaaa gcaatgaaaa tcttgcgtgc
    50761 tcgtgtgtac gataagatcc agcaagaagc acaaagcgag tatgatgcca atcgaaaatc
    50821 agcagtcgga actggggatc gttcagaacg gatccggacg tataatttcc cgcaaaatcg
    50881 tgtgactgat catcgtatcg gattaactat ccaaaaatta gatcagattt tagcagggaa
    50941 attagatgag atcgttgatg cattgatcat gtatgatcaa acatcaaaac tggaagctat
    51001 gcaaaatggg taaaatgacc tatcgagaag tcctgtcacg ggcttcttct tttttagagg
    51061 atcatgggaa agaagcatat agtatccaat ttttattttt ggaacgaaaa caatggaaaa
    51121 aacttgattg gctccttcat atgaacgaag agatttcaga agaagaacag cggttgatcg
    51181 aaaccgatct gcgacttctg cttgcagatc atccacctca gtatttgcta ggatatgcag
    51241 atttttatga tcatcggtta aaggtaactg aagcaacact gatccctcga ccagaaacag
    51301 aggagttagt cgaatggtgt ttagacgaga cgcctggtgt accgttagaa gtgatagata
    51361 ttgggacagg gactggagca attgctatta gtttgaaagc agcaagaaaa aattggcata
    51421 tatctgctgt ggatctttca gaagaagcat tagaagtagc gaaagaaaat gctcagaaag
    51481 aaggaacgaa aatcagtttt tatcatggag atacattagg accagtaatg gatcagcaat
    51541 ttgatgtgat tatttctaat ccgccgtaca tcagtcggaa tgaatgggaa ctgatggatg
    51601 aaagtgtccg gtcattcgaa cctaaaatgg ccctttttgc tgagaacgat ggattagcta
    51661 tttatgaaaa aatagcagtt gaagcaccaa gcgtactaaa atcaaatgga aagatttttt
    51721 tagaaatcgg atttcgccaa ggtgaagcag taaaaaaaat atttcagcaa gcatttccag
    51781 ataaaaaagt ggcgataaaa aaggatcttt ttggcaatga acgaatgatc caagtttatg
    51841 gttgaagttg tggatgaaaa attttttatt tgttttttta ttataaaaaa acacttattt
    51901 gacaaggatg taaacgactt tttggtttat caacaagttt atccactatt gacaaataga
    51961 ttatccacaa acagtggaaa acttgaggat aaactcagac aaaataagaa tgtatgaaaa
    52021 aggggttgtg gataaactgt ggaaacgaaa cgatatgatg aaacagaatt gcaagaggca
    52081 gccgaagcac tgaaagaagg agaacttgtt gcttttccta ccgaaacagt atacggattg
    52141 ggcgccaacg ctcttttacc aaacgctgtc aagaaagtgt tttctgtaaa agggagaccg
    52201 caagataatc cattaatcgt ccatgtcgcc tcttttgaac aagtaaaaga gtatgtggac
    52261 aactttcatc cggcagcaga gaagattgtg aagaacttct ggcctggacc attaactttg
    52321 atatttaaga tcaaaaaaga cactttgccc tcagttgtga cgggaggatt atccacagct
    52381 gcatttcgta taccggataa caaaaaaaca ctagaagtaa tcgaactttc cggtgttccg
    52441 cttgtcggtc caagtgcaaa tacgtcggga aaaccaagtc caacgactgc agatcatgta
    52501 tatcatgatc tacaaggaaa aattaccggg attattgatg atggagctac gcgaattggt
    52561 gtagaatcca ctgttcttga tttaagtgat ccaacagcca tgcctatgat tttacgacca
    52621 ggtgcagtaa cgaaagaaca aatcgaagca gtcatcgaat ctcctgttgc tattgatcag
    52681 catcttgtaa aagaaaatga aacaccaaaa gcccctggga tgaaatacaa acactactca
    52741 cccgatacac gtgttctgat ggtcagagat ggcgattggt caactgcagt tcaatgggca
    52801 aaaaataaga agatccgtgc aggtgtcata gcaagccccg aaattgcgga tcaagtgcgg
    52861 acagatactg cagctgttta tatgtataac gacaactcag tagaagcggc ggctaaagga
    52921 ttgtttgcag gtctaagagg attagatgaa ccaacacttg gattagattt gatttttgta
    52981 caagtgtatc cagaaactgg attggggaat gcttacatga atcgcttgaa aaaagcagca
    53041 ggacaaaatt attttgaaaa atgatcaatt ggttattgtc gcattctttt agtatgatac
    53101 aatgctatca agtaaatctt ctatcagtga acgaaaaact aagcgtaact aatcgtaata
    53161 gaagagatac gaatatttgg caatttgttt ttgatgggaa aaataaaatt gtaggaaaat
    53221 aaaaaggagc tgttgtttgt ggtagattac aaaacgtttg accctgatct ttgggctgcg
    53281 atcgccaaag aagaagaaag acaagaacat aatttggagc tgattgcctc tgaaaacttt
    53341 gtctccgaag cagtgatggc tgcacaagga agtatcttga caaataaata cgcagaaggt
    53401 tatccagggc atcgctatta tggtgggtgt gaatttgtcg atatcgttga aaatttagcg
    53461 attgaccgtg caaaagaatt attcggagcg aaatttgcga acgtccagcc tcactccggc
    53521 tctcaagcca acacggctgc ttatctcgct ttggtagaac caggggacac gatcttagga
    53581 atggatcttt ccgcaggcgg acatctaaca catggatcac cagtcaactt cagtggcaaa
    53641 acctatcact ttgttgctta cggtgttgat ccgacaacag aagttattga ttataatgtg
    53701 gtacgtattt tagctagaaa acatcaacca aaattgatcg tagcaggtgc cagtgcttac
    53761 ggacgaacga tcgactttgc gaaattcaga gaaatcgcag atgaagtagg cgcaaaactg
    53821 atggtagata tggcacatat cgcaggatta gtagcagcag gacttcatcc taatccagtt
    53881 ccttatgcag atatcacaac gactaccact cataaaacat tgcgtggccc tcgaggcggc
    53941 atgattttga caaacgatga agcattagca aaaaaaataa atagtgcggt atttcctggt
    54001 attcaaggcg gccctctaga gcatgtgatt gcgggtaaag cagtagcatt caaagaagca
    54061 ttagatcctg catttaaaga atatagtgaa caaatcatcg ccaatgcaaa agcaatggtc
    54121 aaagttttca atcaagcaat tggaaccaga gtcatttcag gggcgacaga taatcatctg
    54181 atgttgatcg atgttcgcga gttaggtata aatggaaaag aagcagagag cattttggac
    54241 agtgtgaata tcactgtaaa taaaaactcg attccttttg aaacactaag tccattcaaa
    54301 acaagcggta ttcgaatcgg cacgccagca atcactactc gcgggttcaa agaagaagat
    54361 gctgtaaaag tggctgaact agttgtcaaa gcattacaag caaaagatga taacgctcaa
    54421 ctagacgaag tcaaaacagg tgtacgcgaa ttaactgaaa aatttccgtt gcataaaaaa
    54481 taaaaattta aattccatta gtaaacagaa aagttcaatg ttgggctttt ctgtttattt
    54541 tttgggaaaa cggattcttt gacaatatcg gtgatgctct ctagtatatg aggtgtaatt
    54601 ttagtcttca cactaaatct cagggaggaa aagtcatgac tgatcgtatc tcgttagaaa
    54661 aggaagcagt agcattcagt gatgcaaatg cgcctcatcc gcgtatctat gaactagcac
    54721 cggaagaagg aagagtttta ctagaagaag tacaagaatc acctgtgaac aaatattccg
    54781 tcgatattga agacttgatg gtggatactg gcgaatgggg aaaactcaat attcgtctcg
    54841 tacgaccaga agggaatatg gataaattac cagttatttt ttatattcat ggagcaggct
    54901 gggtttttgg cagtgctcaa acccatgata aattgatcag agaacttgcg gttcgtacga
    54961 attccgtcgt tgtctttcca gaatatagtc gatcaccaga agcaaaatat ccaacagcaa
    55021 ttgaacaaaa ttatgctgtg ttgcaacagc tgaataaatt agccaagtca aaaaatctgg
    55081 atctcgagaa tctaatcgtt gcaggagatt cagtaggtgg taacatggct acagtcatga
    55141 cgatcttaac aaaagaacga aagggcctcc caatatccaa gcaattgctg tattatccag
    55201 tcacaaacgc tgattttgat acagattcct atcatgaatt cggagaaaac tattttttga
    55261 caagagaagg aatgatgtgg ttttgggacc aatatacgac gaatgaagaa gagcgtgctg
    55321 aaatcacagc ttcgcctttg agagcaaata taaatgaact aaatgatctt ccttcagcaa
    55381 tgattttaac gggagaagcg gatgtattaa gagatgaagg tgaggcttat gcgcgcaaac
    55441 ttcgtgatgc aggggttcct gttacacaag tacgcttcca aggaatcatc catgattttg
    55501 tcatggtaaa tgctatggat cagacaaagg caacgcgtgc agcaatggca ttatctactt
    55561 cttggatcaa gaaaaaagaa taagctttgc ttgtccgcag gaaacttctt ttcaaaaacg
    55621 gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg ttttacaatt
    55681 aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa gtgatcgatc
    55741 atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga acaaaggttt
    55801 ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca cgggatatgc
    55861 cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa acgttgtctg
    55921 gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt gacggtatct
    55981 tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat gaagaaacat
    56041 tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt cagttgtttg
    56101 tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat gcattgaaag
    56161 aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag ggagtaaaag
    56221 ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat gaaaaattga
    56281 atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg tttggtacaa
    56341 aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt ttggtcattt
    56401 ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa aaaaccatcc
    56461 caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt aacgattact
    56521 acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat ctcaacaaca
    56581 gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg aattgtgaac
    56641 attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat agttgtatca
    56701 aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta taccacgttt
    56761 tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa tcagccaaat
    56821 agagtatata atttcttttg gataaggcca gcttagctca gttggtagag caacgcactc
    56881 gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa gacagatata
    56941 tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat aaagtatgct
    57001 ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa acgactagca
    57061 atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc gagttgcttt
    57121 taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg atgtgtgcta
    57181 cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca aagatgattg
    57241 ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg agaaaataca
    57301 ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat aacgggctga
    57361 aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc aaaaataccg
    57421 agactgcggc actgattact acattgataa aaaaatattt ggctatattt ttgacaggag
    57481 cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt gtaaatgcta
    57541 taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcg
//
LOCUS       DAHEYX010000002        46808 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHEYX010000002 DAHEYX010000000
VERSION     DAHEYX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.002"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            195..1067
                     /locus_tag="MB219_000056"
     CDS             195..1067
                     /locus_tag="MB219_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8425799.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(1525..3723)
                     /locus_tag="MB219_000057"
     CDS             complement(1525..3723)
                     /locus_tag="MB219_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8425800.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            3955..4947
                     /locus_tag="MB219_000058"
     CDS             3955..4947
                     /locus_tag="MB219_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8425801.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGVYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            5007..6428
                     /locus_tag="MB219_000059"
     CDS             5007..6428
                     /locus_tag="MB219_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCC8425802.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            6421..7920
                     /locus_tag="MB219_000060"
     CDS             6421..7920
                     /locus_tag="MB219_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCC8425803.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            7959..9353
                     /gene="uxaC"
                     /locus_tag="MB219_000061"
     CDS             7959..9353
                     /gene="uxaC"
                     /locus_tag="MB219_000061"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCC8425804.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            9512..10195
                     /locus_tag="MB219_000062"
     CDS             9512..10195
                     /locus_tag="MB219_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCC8425805.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            10197..11033
                     /locus_tag="MB219_000063"
     CDS             10197..11033
                     /locus_tag="MB219_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCC8425806.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            11044..12798
                     /locus_tag="MB219_000064"
     CDS             11044..12798
                     /locus_tag="MB219_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8425807.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(13074..13415)
                     /locus_tag="MB219_000065"
     CDS             complement(13074..13415)
                     /locus_tag="MB219_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCC8425808.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(13483..15654)
                     /locus_tag="MB219_000066"
     CDS             complement(13483..15654)
                     /locus_tag="MB219_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCC8425809.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            15823..16722
                     /locus_tag="MB219_000067"
     CDS             15823..16722
                     /locus_tag="MB219_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCC8425810.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(16793..17650)
                     /locus_tag="MB219_000068"
     CDS             complement(16793..17650)
                     /locus_tag="MB219_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCC8425811.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(17635..20337)
                     /locus_tag="MB219_000069"
     CDS             complement(17635..20337)
                     /locus_tag="MB219_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCC8425812.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(20350..21639)
                     /locus_tag="MB219_000070"
     CDS             complement(20350..21639)
                     /locus_tag="MB219_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCC8425813.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            21789..22835
                     /locus_tag="MB219_000071"
     CDS             21789..22835
                     /locus_tag="MB219_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8425814.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            22837..24990
                     /locus_tag="MB219_000072"
     CDS             22837..24990
                     /locus_tag="MB219_000072"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCC8425815.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(25463..26914)
                     /locus_tag="MB219_000073"
     CDS             complement(25463..26914)
                     /locus_tag="MB219_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8425816.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(26911..28635)
                     /locus_tag="MB219_000074"
     CDS             complement(26911..28635)
                     /locus_tag="MB219_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCC8425817.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(28628..29242)
                     /locus_tag="MB219_000075"
     CDS             complement(28628..29242)
                     /locus_tag="MB219_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCC8425818.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(29587..31044)
                     /locus_tag="MB219_000076"
     CDS             complement(29587..31044)
                     /locus_tag="MB219_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8425819.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(31085..32005)
                     /locus_tag="MB219_000077"
     CDS             complement(31085..32005)
                     /locus_tag="MB219_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8425820.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(32017..32964)
                     /locus_tag="MB219_000078"
     CDS             complement(32017..32964)
                     /locus_tag="MB219_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8425821.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(33443..34162)
                     /locus_tag="MB219_000079"
     CDS             complement(33443..34162)
                     /locus_tag="MB219_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCC8425822.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(34211..35857)
                     /locus_tag="MB219_000080"
     CDS             complement(34211..35857)
                     /locus_tag="MB219_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCC8425823.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            36036..36626
                     /locus_tag="MB219_000081"
     CDS             36036..36626
                     /locus_tag="MB219_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCC8425824.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            36718..38223
                     /locus_tag="MB219_000082"
     CDS             36718..38223
                     /locus_tag="MB219_000082"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCC8425825.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(38307..39659)
                     /gene="celB"
                     /locus_tag="MB219_000083"
     CDS             complement(38307..39659)
                     /gene="celB"
                     /locus_tag="MB219_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCC8425826.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(39659..40177)
                     /locus_tag="MB219_000084"
     CDS             complement(39659..40177)
                     /locus_tag="MB219_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425827.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(40193..40519)
                     /locus_tag="MB219_000085"
     CDS             complement(40193..40519)
                     /locus_tag="MB219_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCC8425828.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(40532..42511)
                     /locus_tag="MB219_000086"
     CDS             complement(40532..42511)
                     /locus_tag="MB219_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCC8425829.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(42532..42846)
                     /locus_tag="MB219_000087"
     CDS             complement(42532..42846)
                     /locus_tag="MB219_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCC8425830.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            43014..43307
                     /locus_tag="MB219_000088"
     CDS             43014..43307
                     /locus_tag="MB219_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCC8425831.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(43385..44497)
                     /locus_tag="MB219_000089"
     CDS             complement(43385..44497)
                     /locus_tag="MB219_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCC8425832.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            44884..45063
                     /locus_tag="MB219_000090"
     CDS             44884..45063
                     /locus_tag="MB219_000090"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425833.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            45257..45427
                     /locus_tag="MB219_000091"
     CDS             45257..45427
                     /locus_tag="MB219_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8425834.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(45851..46798)
                     /locus_tag="MB219_000092"
     CDS             complement(45851..46798)
                     /locus_tag="MB219_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HCC8425835.1"
                     /translation="MQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTS
                     SGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        13999 a         9450 c         8353 g        15006 t
ORIGIN      
        1 tagcaatgat ttttgtgcta tacaaaacga taaagtgtat tttttcacaa ataaactacg
       61 catagcaaac cttctatttt tttctatctg acacttagca taggaaatga ttatatacgt
      121 aatctttcac cgatgataaa ttgaacatat agaaacagaa acatttaatt tattttgagg
      181 aggagcctat ctatatgaaa aagaaagtct ctataggaga aagaagtttt acacttttca
      241 acaatacgtt tctaattttg ctagctttga tctgtatcgt tcctttttta aatatcatcg
      301 ctacatcttt tgcttctaca caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta
      361 ccttttcttt agacgcgtat cgctacattc tttctacgcc gaccattttt agagcacttg
      421 ctgtttcgat tggtgttaca ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga
      481 tggcctacgg tctttccaga aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg
      541 tattttctat gttgttcagc ggggggatga ttccaacgtt cttagttgtt cgttctctag
      601 ggctggtcaa ttcttactgg gccatgatcc tgcctgtggc tgttaatgca atgaatatga
      661 tcattatgag aaactttttc caagccttgc cggatagttt ggaagaatca gcaaaaatgg
      721 atggttgcac agattttggt gtatttttca aaattatgct gccattagcg ttaccgtcta
      781 ttgctactat ctcgcttttt tatgctgtta cttactggaa cacttatatg actgctattt
      841 tgtatatcaa cgattcatct aaatggccga tacagattct tttacgccaa atcgtcattg
      901 tatcaagtgg gatgcaagca gaaagtagtg ccgttgatgt gattccacca gcacaaacag
      961 ttaaaatggc tgttatcgtt attgcaacag tgcctatgct gattgtttat ccttttgttc
     1021 agaaatattt tgtcaaagga gcactcgtag gttctgtcaa agggtgatta tgtactgaac
     1081 gcaaaatggt agttgtgtca gaagtggtca gcttcaagaa ataagaaggc atttacgaaa
     1141 attgcttctt aaatttttgt gaaatttcgg cttatttttc gaagaatcta ctcctggaac
     1201 accgtgtatc agaggttgtg aaagagacgt tcagccttga gcgatgataa aaactctcac
     1261 aaacattgcg acgagtaacc cacaggagca ataagaagcg attttgaaaa atagcttccc
     1321 atatttcaca aaattatgcc ttattgtcga tagcctgtct cttgaacgcc atttattcgt
     1381 aaaaaagaac tgtgacaaga cttttgtcac agttcttttt gcgtgcttag ccttgaaatt
     1441 taactatcca taaatacaaa tactagggtt attctaactt cttttattgc ttgaatcagt
     1501 ccacaagaaa aacagcctct tgattcacaa atattttttt cgatattctc ccggcgtaat
     1561 cgattccttc tttttaaaga aacgaataaa attttgcgga tttcgatatt gcaatctttc
     1621 tgcaatctct tttacggaga ggttagtctc tttcaaccat ttttttgcta cctctaaacg
     1681 atgattttga acaaaatcac caaatttttc tcctgtttcc tttttaaata cattacttaa
     1741 atagttaggg ttataatgca acctatcagc gatcacatct aaagacagat cacgatcata
     1801 ttctgtgtag acgatatgca taatattctc cgacaaattt ttaaattcat gatcagtgat
     1861 tgcttgagag cattctgtga ttggcagaac taattgttct aaaacaagtt tttctagttc
     1921 tttgggatga taattgttca gtgcttttac ataaatttgt ttcatgttat caaagttttt
     1981 tgaatctact cccaataact gtcctaattg aacgagttca ttgatcaagc ggaccgaaac
     2041 tacttcccga ctcaaaggat ttttattttg tgcaaacaat gcatcaatca attcatgtac
     2101 taaattcttc acttcctgat ttccagaacg aatggcttca aacaatcgat tttgttcttc
     2161 tatcggatat ctaataagtg ttttttcatg agcagcagga acgatttcct gataaaaaat
     2221 aattgaactt ggaccagtat ttacacgata atacagggct tcttttgccc gatcaaccga
     2281 ttgcttgctt tcattcagcg tttcgaaccg gttactgatg cctatactga tagttaaatt
     2341 caagtattct tttacacgtt tttgaatgag tcggcaatac tccatgactt ttttgcttgc
     2401 ttctgcatca tctttatcca ttcgataaat agtggcttgc atttcttcat tcaaaacgat
     2461 cggcagcatt cgattttcct ttggcacaat ttccgtgatc atgttgttga tcgctaaaag
     2521 tagcaaatca cgctccccac cgtgactatc ttctaacaga tcgatttgaa ccaaagctgt
     2581 ataatagcag tcattttgag aactataacc aaattgctgc aaccgctgat ctagttctcg
     2641 cctttctact cgatttctaa ataaactaag tacaaatagc gtttccagct gtggcttttg
     2701 ggtaactaag tgtgcactta acgcttcttt ttctcctatg atccgactga ctgattgagc
     2761 gaccaaatct agttctttcc cactgaatcc ttttgattta agattcagac gttcctgtat
     2821 ttgagaaatc ggacgggcaa atctgtccga aaataagtag gaaagaagac caatgagtcc
     2881 taccagtgtt aaagacactg caactagccc gattctcaga ttgcggatca ttgcgccaat
     2941 tgcactagag tcaacttcta atctgtaaat ccaattatta taatctgatt tcagtactat
     3001 atacgtttta tctttttctc ttatcacttt cagctgattg taatctagat ccacgtccgt
     3061 taatacagtt ggttgctctt tattttttac tgtctgagta gtaaaaaggt tgccaacttc
     3121 gttttcaata gaaagtatgt cttctccact ttcaccaaca acttgttcta ttgtatgttt
     3181 ctctatcgta gcaattccca atgcatattt ttctttttca aacattggca aagaaatcac
     3241 catttcgatt cctttgtttt tcttttgcca aaataaatta tttttgttat ttatgtactt
     3301 ctgttgatac ttttcaattt cctcttcggt cagttgactc aacgatccat tgattactcc
     3361 ccatttacca gccaaactga ctaaatcata attattatca tccataacaa tcgtttctat
     3421 aaaatttagt tctttcctga tttcagaata agtggagtaa tctgtatatg tcagcggatg
     3481 cttgaatatg tccgagaaat ttttggtcga accatacgta gaaaacgaat attcaatcgt
     3541 tctaattttc cattcaacgt tagttttaac ttgcctgagc aaattctttt tgtctgttaa
     3601 ataactcgat tcgatcgatt cataggtatt ctggtaaatg aataaactaa atcctatgac
     3661 aagtacggct cctaataaaa acatcggtaa aaatatttta taaaatactg tacttttctt
     3721 cattttcttc ctctcctcct tttaaaataa aaggctttca tttttctgta aaattgatac
     3781 tcatctaatt aaatgagcat tccccgcttt caagaatagc ataattaaaa ataaaaatcc
     3841 aagtaatttt taacgtgtgc aatcttttca tatcaaagat ctattgactt atcgtagtta
     3901 tcgtaatatg atgaatgcgt taacaaaaag tgaaaaagta aggaaaatga tggaatggta
     3961 acgatcaaag atgtcgctaa agcagctggc gtttctcata cgacagtttc tcgtgctttg
     4021 aataacagca gtttaataaa aaatgaaaca aaacaaaaaa tacgacaact agctaaggaa
     4081 atgaattatt cgccaaacta tagtgcaaaa ggtttagtga atcaaaaaaa atatctaatc
     4141 ggactatttt tctcaagttt gcataaagga acttcatcaa atttcctagc agattccatt
     4201 gcaggcatcc attcagtatt agacacaaat tacagtttat ctgttgaaag tatcgatgtt
     4261 gttgatttaa cagaaatcaa cctgcaacgc tacgatggac tgatcgttat gagtcaaagt
     4321 gatgatgatc agcctttcat cgactatctc aaagaaaaag aatttccttt cgttgttgtc
     4381 aatcgtcaca tcaatgatcc agcggttatc aacgtcactg ccgatgatgc ggccggtgtt
     4441 atgcaggcag tagattacgg tattggatgc ggacatacga agatcgctta tattggcgga
     4501 aagaaaaatt ttcgttcgac aaaagaacgg gaaaaagggg tattgaaaag tctaaaaaaa
     4561 gcgagcatcc ctgtcaatcc agcctatttt ttaacaggag tttacagctt agaaagcggc
     4621 ctccaaaaca tggaatacct attatcactt tctgacctgc ctactctggt tttttgcggc
     4681 aacgacgaca tagcgattgg tgcattgaga gctgtacatt tagctggcct ccgtgttccc
     4741 cagaacattt ctctgatcgg gttcgatgat tctccagtcg tctcctatct caatccgccg
     4801 cttaccaccg tacgtaagcc tttgaaagaa atgtgtcaag aaggaacaaa gcttttacta
     4861 gatttgatcg aaggcaggac gatcgaacaa tctaaaattg agctctccac cttgttgatc
     4921 cttcgtgagt cgatcaatta cttgtaaact acaagtaatt ttatataaaa atgttaacgt
     4981 gtgcataaca cagaaaggaa gtttatatga aaaaactgac aaaagaatta gtgaaaaaaa
     5041 acgaagcacc tgtaaaagtt cttcagtttg gtgaaggcaa ttttatgcgt ggatttattg
     5101 attggcagat tcagcagctg aataaccaac aattgtttaa aggtaatgtt gcaatcattc
     5161 aacctttaga acaaggtctt ggaaatatga tgaaccagca agataattta tatacggtca
     5221 ttttacaagg actcagcaat ggcgaaatcg ttgatacttc cgagattatc accgtagtag
     5281 aacaaatgat caatccttat gatgaatggg atgcttatct atctctagca gaaaatgacg
     5341 acttggccta tatcgtctcc aatacaacag aggcaggaat ccaattcgtg gagactgata
     5401 atttaacaga tactccccca cagagcctcc cgggcaaact gacagccttt ttataccgac
     5461 gtttcaaatt aaacaaagct ggctttacca tcattccttg cgaactgatt gacagaaacg
     5521 gcgaacgtct aaaagaaatc attttacgct acgcagaaaa atgggattta gaacctgctt
     5581 ttgttgagtg gttagaaaaa gaaaatgttt tctgttgtag tttagttgat cgcatcgtcc
     5641 caggttatcc tcgcgaagat gcaggtgttt tcaatcaacg gcataactat gaagatcaat
     5701 tgatggtcaa agcagaacca ttcatgcttt gggtgatcga agggcctaaa gaattggaga
     5761 atcaactacc attcaaacag gctggcttga atgtcatcat cacagatgat atgactcctt
     5821 atcgtgaacg taaggtgcat ttgcttaatg gaccgcatac agcgatggtc cctcttgctt
     5881 tgttggcgga attggaaact gtagaagaag taatgaaaga tcctttattc tccgcatatg
     5941 ttgatcagtt attcaatcta gaattgattc ctatgctttc tttacctaaa gatgaattag
     6001 ccatttatgc tgaccaaatc aaagaacgtt tcttgaatcc ttttgcccat cacaaattag
     6061 aagcgatttc attaaatagt gtttcaaagt tcagcacccg tctgcttcca gtgttcaaga
     6121 agtatatcga agaacaaaat caggtaccac cgttaatcac tgtttcacta gctgcgctac
     6181 ttcttatgta tcgaggcgat caagtaaagc cacatgatga tgaaaagacg ataagcgaat
     6241 ttactgatgc ttggtctgat aatggaacag ctattcctcg attattgcaa aacgctgcgc
     6301 tttggggaga agatttgtct caaataccga atgtgacaga tacagttcaa gaaatagctg
     6361 caaagatcga tcaaaaaggt gtccggtcag tattacaaga aatgaaagga acgatggtcc
     6421 atgcttgaaa ccctgcaacc aacaatgaaa ttaaatcgac gagacagtgt ggtcgtcgca
     6481 ttaaccccta ttccaaaaca aacacaactg acgattgatc agcaaacgat caccactctt
     6541 gaagatatcc cgcaaggtca taagattgca ttgggtgatt tgaaagctgg agacaacgtg
     6601 atcaaatatg gctatccaat cggtcatgtg acgacagacg taactgccgg tcaatggctg
     6661 cacactcata atgtaaaaac caatctttct ggtgaattgg attatcgata tgaaaaagat
     6721 gtccatccaa gtcattacct ttttgagaac cgaacatttc aaggttactt gcgaaaaaat
     6781 ggaaaagtag ggattcgtaa tgatttgttt ttagtaccta ccgttgggtg tgtgaatggc
     6841 attgctgaac tgatcgtcaa acagttcaag gaaaaacatc cagatctggg acagtttgac
     6901 catattacga ttttaaagca tccttatggc tgttcgcagc ttggaaaaga tcatcaaaat
     6961 actcgagaaa ttttagcgga cgcagtaaat catcccaatg ccggaggagt tttagtattt
     7021 ggattaggtt gtgagaacaa tacagttcct gaattcaaaa aaattttagg tgaatatgat
     7081 gaagaacgag tgaaatttct agtggctcaa gatgtttacg atgagatcga acaaggtgtg
     7141 gctctattag aagagctttt aacagcggcg gaaaatgacc agcgcatatc agtcccgctc
     7201 tctaagttga atgtcggttt gaaatgcggc ggttctgatg gtctatcggg aataactgct
     7261 aatcctttac ttggtgcatt ttctgactac ttgattgctc aaggaggcag cacgatctta
     7321 acagaagtgc cagaaatgtt tggcgcagaa caagtattga tggcacgagc ggaaaatgaa
     7381 gaagtttttg actcaatcgt tgacttgatc aatgacttca aacagtattt cttgtcttac
     7441 ggggaaccag tttacgaaaa cccatctcct ggtaataaag caggtgggat cactacatta
     7501 gaagataagt ctttaggctg cacgcaaaaa tcaggaagct ctcctgtcgt tgatgtgcta
     7561 caatatggcg aaaaaatcag aaaaccaggg cttagtttat tgcaagctcc cgggaatgat
     7621 cttgtagctg cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga
     7681 agagggactc cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt
     7741 tttgaacgca aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg
     7801 gaagtaatct tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg
     7861 aaaaatgaac aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga
     7921 ttttcataaa acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt
     7981 tattaaaaga cgattgggcg aaaaagcttt accattccta cgcaaaaaac atgcccatta
     8041 ttgattatca ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc
     8101 tgacagaagc ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg
     8161 tgcctgaaga aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc
     8221 aaaccgtacc aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc
     8281 gtttcttcgg tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag
     8341 caaatgaaat tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg
     8401 tagaagtgat ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg
     8461 ctcatgaaga accagatttg aaggttctac cttcattccg tccagataaa gcactcaata
     8521 ttgaaaaaga aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga
     8581 tcaccaccta tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac
     8641 atggatgtcg tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa
     8701 cagtattaga agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa
     8761 tcgatgctta tcgtacagaa acactgatac atttgaccca cttctatcat tctcataatt
     8821 ggacgatgca gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat
     8881 tagggcccga taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa
     8941 aattattgaa ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga
     9001 atccgaatga ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg
     9061 gaaaactgca gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc
     9121 aattgacaca atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt
     9181 caagaagttt cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt
     9241 taggcgaaat ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag
     9301 ttcaagcgat tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta
     9361 atagtaacag taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt
     9421 tttagagaag aagacggacc aaatttgctc cgtcttcttc tcttgctgtt ctgcttcatt
     9481 gcctataaac ctttatgaaa gaagagataa catgcctact atccatattg ccggtgattc
     9541 tactgcggca gaaaaagatg aaaacaagcg tcccgagaca ggttggggag aaaaaatctc
     9601 tgcttatttt tcacccgaga tccagatcaa taaccaagca aagaatggac gcagcaccaa
     9661 atcatttatc caggaaggaa ggttagcccg tctagcttca cagtttcagc cgaatgattt
     9721 cttatttgta caatttggac ataacgacca gaaaatccaa gaagaaagag gtacgcttcc
     9781 cttcggtagc tatttaaaaa atctgcagat attttttgaa tgcgcaaaaa aagcgaacgt
     9841 ccaaatggtc ttactgacat ctgtgacgcg acgagattac ttagaaaacg gaacattgaa
     9901 tccagatata cttggagact atccaaaagc gatgcgtgca tttgctgaga aacaccatat
     9961 tcctctttta gatgtgttct caagatcaca agaattgttc cagaccttct caaaagaaga
    10021 gacaaaaaaa ttctacctgc atcttatacc tgatacttgt aaaaattacc cagaaggact
    10081 aaaagacaat acccacttta gtccagaagg cgctgataaa gttgcccgaa taattgttga
    10141 attgataaaa gaatcccgtc tgcctcttgc taattattta caaaaaggag tttgagatgt
    10201 ttccattaaa tttaggcatc cgtgcccacg atttagatgt tcgaaatcgt gaagaaatcg
    10261 ttcaaaaagt atcacagctt cacttgtccc atatccaatt tgcaccacaa aaagcatttc
    10321 ctgaacaact taaattaact gacatgacac ttgggagtgc tgcctatttt ggtgaatact
    10381 tccaaaaaaa caatatcgaa ctttccattt taggatgcta tatcaaccta tccagcaaaa
    10441 atcaagaagt tcgtgcacaa gcattggcaa ctttcaaaca gcaaattgct ctgtgctctg
    10501 cctatcaagc caagatgatt gctacggaaa ccggtagtgt ctctgtcggt tacacaaaag
    10561 aaaattttac tgaagaagct tatcagatcg ccagaaattc tatcattgaa ttagtcgctc
    10621 atgcagaaaa ttttggcgta actgttgctg ttgaagcagg gatcaatcat ccaatctata
    10681 actatcagtt agctaaacgt ttaatcgatg aagtagcttc accaaattta aaaatcattt
    10741 tagattgtgc gaatttgatg cataaagaaa atcatgctga tcaggaaaaa gtgatccgag
    10801 gtgccttgga caatttacat ggttatatta cagcgcttca tctcaaagac tatatcatgg
    10861 aaaatggaaa gatccgcatg gtacctgttg gcaagggctg tttagatttc cgcccggtac
    10921 tgcattatat caaatatgag cgtcctctaa tgtacgccac tcttgaagct actcctgaga
    10981 tttatgttcc tgaagcaata gaacacttgc agtttattta ccaagattgt taaggagaag
    11041 attatggagg aacgaataca gaaaagttat caaaaatatc atgcaaatgc ttttcagaca
    11101 ttgctgcatc tttatcaggg aaattattgg cgttttgcca tgtcaggact cttttttatt
    11161 atcaaacatc ttccatcttg gttaatgccg atcgctattg ccaatgtaat caatgcagct
    11221 tcatcgaaag attcttctca gttgcaggcc atttatttca acgctttttt gatggcttgt
    11281 ctgattggtc aaaatatatt gacgaattat tttcatgtaa aataccatag cacttcgatc
    11341 cgtcaagtag aagctggact aagagcgacc cttatcaaga aaatccaaga actatctatt
    11401 ccttatcaaa aagaattgca atctggacga atccagtcaa agatcatccg tgatgtagaa
    11461 gctatccaaa cgttgtcctc tcaagtattt gtcagcggat taaatatcgc tgtcaattta
    11521 ttcgtagctt taggtatcac gctttataaa agtcctgttg ttttcatgtt tttcttattg
    11581 actgttcctc tcgccgcatt cgttactctc tttttccgta aaaggatcag acagtccaac
    11641 tcagacttca ggcaagaaat ggaagagact tctgcaaaag tgatcgaagt gatcgaatta
    11701 gcaccgattg ctcgagctca tgggttagaa aaacacgaaa caactcgctt aaataagcat
    11761 tttactcaaa tctatgaaaa aggattccac ttagatatcc tgcaatctgt tttcggatca
    11821 gtcagctggg ctagttttca gatatttcag ctgatttgtt tgattttcac tgggatatta
    11881 gctttacaag ggcgcatcca acccggcgat gtcgtgatgt atcagacgta tttcacgacg
    11941 atcgtcaatt ccgtttctgg aatgatcacg ctgattccta cgatttcaaa aggcatggaa
    12001 tctatcactt ctattggcga actgttgatt gctggcgatg tagaaactta tcacggtaag
    12061 aaaaaaatcc aacatttgga tggaaatttt cgttttgagg atgtttcttt tctttatccg
    12121 gactcactca aaccgattat cgatcatttt tctctagagg tgaaagctgg agaaacaatt
    12181 gcttttgttg ggccatctgg ctctggaaaa tctacactcc ttaacttatt gattggattt
    12241 atccagccga cttcaggaaa aatttttcta gatgatcagc ctttggatga actggatatg
    12301 cgaagtgtgc gaaattattt agccgttgtt cctcaaacca ctttgttgtt ctccgcttcg
    12361 atcaaagaga acatcactta tggcttgaaa aacgtatcaa aagaacgttt agatgaagtc
    12421 atcgaagctg cgcaactttc ttcactgatt gaccagcttc ctgatggtct ggatacctta
    12481 gtaggcgaac atggcaacaa gctgtctggt ggccaaaagc aacgtatctc cattgcacga
    12541 gcgttgatac gtcagccaaa aatcattttg ctagatgaag caacctcagc tttagataac
    12601 cagtcagaga aaaaaatcca gcaagccctg aattacctga cagaaacacc cactactttt
    12661 attgtggctc atcggctttc aacgatcaaa gaggcagata aaatcgttgt gatcgatgaa
    12721 ggaaaaatcg ttgaaatagg tacttacgaa aaactgttag ctcaaaaagg ctatttctac
    12781 cgcttgaaaa atgaatgaca aaaaatagga gctatgacaa gacttttgtc gtagctccta
    12841 tttctgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcatcaa
    12901 acatgagaag ctgttttttg aaattgttcc ttgtaactca gagttaaacg gtaggctcac
    12961 gacctcttgg taaatggtgt tcaagagaca gcctatcggc aataagaagc aattttgtca
    13021 tcgctcaagg ccgaacgtct ctttcacacc ctctgttgtt tcataaaata gttttattcg
    13081 ctgtaaaggt cgcgaactgg tgtcatgatg atacggttca agtcattgat gattgtactg
    13141 aagccttgct ctttttgcat caagtcattg attacttctt ctgattggat tttcacagcc
    13201 atttcttgtg ctttcattgc atcttcgtcg ctgaactctt gtccactcat ttgtttttct
    13261 tgtaattcga tttgcatcgc ttggaagtct ttgaacagtt tgtgtgcttc ttcgtttgct
    13321 tgtactttct cgtacgcttc tttcaacgct ttgaattctg gtaattcacg gatctcacgt
    13381 tcgatttgat tcgcgctatc gtagatgttt gacatgttat gccctctttt cttttagtag
    13441 ttactctcta ttgtaacatg gggttattcg ttttcaaagg ggctactctc cttgatattt
    13501 ccgccagagg tctttagccg cttcaccaat tccctgaacg ccttctttca ccatatcgcc
    13561 gaagtttttc agcccttctt ttgcttgttc accatattga ttcaagttat cttgccaatt
    13621 tccagactct ccattagaat ttcctgtgtt tccaacttga tctgcagcta ccacttgtcc
    13681 tcctgtggca taggcatctg ctacactaaa tcctgttttt gaagtatacg gaaggatgcc
    13741 ttctgccgct gatttgaaga ctttcccgac aacatttcca cttgtccctt ccaaatagtg
    13801 cagcttgctg gattcttcgt aacccatcca tgtcgaaatc actacgtctg gcgtgtaacc
    13861 gatcatccat tggtcatttg cttttgtggc atcgaaatta gtttctgtgg ttcctgtttt
    13921 ccctgctatc gtataacctg caggattcgc agcagccgct gttccatttg aaaatgtacc
    13981 aagaagcatg ctggtcattt catcggctgt ttcctttgat atgacctgtt ttggtttttt
    14041 gtttgtgtta tcgacgatca ccgctccagt cgaatcaacg attttcgtga taagatgcgg
    14101 cgtatacatc tgaccatcat tggcaaatgc actgtaagca cccgccatga tcaatggaga
    14161 tactcctttt tccagtccac caagggctaa gccataatac ttatcagatt ttgctaaagg
    14221 caggccgaat tcttctgctt tatcatatcc tttttgtaaa ccgatctcat gcaaaagcca
    14281 tacagtaggc aaattcaaac tttgtgcaac agcttgatac atcggtactt ctccttggta
    14341 tgtcccgtca aaattatgcg catcgtaata actttgcggt tcgtctttca agatactgga
    14401 aggcttgtat ccagcttcca gtgctggtgt atatacgctt aatggtttga tcgttgatcc
    14461 tggtgaacga cgcatctgtg tagcaaagct gaatcctcta aagacatgat ctcctcttcg
    14521 tcctactagt gcttgtacac cacctgtatt cggatctaaa gctactgaag cagattggac
    14581 catggcacca tctgaagcat ttgctgggaa taatgcactg ttttgataag tagcttctaa
    14641 ttgcttttga taatcctgat ctaaagctgt atagatttta tagcctttat tcatgacatc
    14701 ttcttcatca agaccatact cgttaactgc ttcatcgatc actgcatcaa aataataagg
    14761 ataacgataa ccagaatctg aattatcgta tgtgtcatta agatacgcgc tgatatctgt
    14821 ctggctttct gcttgttctt gttcttttgt gatttttccg ttttcttcca ttacacttag
    14881 tactgtgttt ttccgatcat tcgcattttt tgggtgatcg atcggattat aaataccagg
    14941 acctttcagc attcctgcca aagtagcagc ctcagacagc gtaacattcg ttgcatctac
    15001 tccaaaatac tttctggaag cgtcttggac accccatacc ccgttgccaa aataggcatt
    15061 gttaagatac atggtcaaga tttcttcttt gctgtacttc ttttcgatct cgattgccaa
    15121 aaataattcc ttcgcttttc gatcaaacgt ttggtctaat gtcagatacg catttttggc
    15181 aagctgctga gtgatcgtac ttcctccgcc ccctgatgtt ccgccagaca agaccattcg
    15241 gactgctgca cgggcaatcc ctttgatatc aaacccgtga tgctcgtaaa aattcctatc
    15301 ttctgtcgag attactgcat cttggatata gggagaaatc gcgtttaatt ctacatatgt
    15361 tcctttttgg gcgtacagtg tgccagcttc ttcgtttttc ttatcataaa tgactgtcga
    15421 ttggcttaat cctgatttta acgtttcgac atttgcttgt tttgctaaaa taaagaggta
    15481 gatacttgtc actaagacac agatcaaacc aatcagcaaa atcaatttgt tcacatgata
    15541 ttttttccag atgcgctttc gccatcgatg gaattgtccg agatatggtc tgatccaacg
    15601 ccaaaaactg atcaaagcag cttttatctt ttctaaaatg ctttgaaaat ccatttgctt
    15661 cttctcctgt ctatttgatt caatcgtctt attttaccat agtgaaagag tgttttcttc
    15721 ctgcttaagt tgtagttcaa tatgtttttt tacgttagtg caagtagagt aacataagaa
    15781 tgaacagaaa aatgtaagtt ttttcttaaa tatgttttaa aaatggtaca attagtctgc
    15841 gattgcactg aaaggagtac acttgtgaaa atcacgatca ctttacctat gaaccatcct
    15901 gaaacaacaa ttcgcgactt gcttgaaaag gaatggttag ttcctagaaa ggttcgacac
    15961 tttttgcgta tacgtaaaaa cgtttggatc aatcaacaac ctgctttgtt ccatcatgaa
    16021 gtaaagggca atgaccagat cacattgtta ttcgaagaaa cagattataa ttatcaagaa
    16081 gttcaattag gagatgcgaa gcacatccgt gttttatatg aagatgagca tttgattata
    16141 gtggataagc ctgcaggaat gaagactcat ccaaatgaac cgacagaaaa cagtacctta
    16201 ctcaatcatt tagctgctta tctcgacact aaacagcaac gaccttacgt tgtccaccgc
    16261 ttggacaaag aaacaagcgg tgccgtttta tttgcaaaaa atccatttgt cttgccgata
    16321 cttggaagaa tgttggaaca aaaaatgatc tatcggagat atcaagccat cgtatgggga
    16381 gaaattgcca aagatatgac catcaccaat aaaattggac gagatcgcca tgatcggaga
    16441 aaaagggtcg tagatgataa aaatggtcag agcgccttga cacatgtcac tgtcaacaaa
    16501 gtactttctg aacaaacaca agtctattgt gttttggaaa ccggacgtac acaccagatc
    16561 agagttcatt tagctcataa gggacatccg atcgttggag accctctcta ccaaaataga
    16621 acagctgaac gactgatgct ccatgcatta gaactgcaca tgatccatcc atttactcaa
    16681 gaaaaaatag tcggtaaagc tttacctgga ttatgggaat agaaataaaa aagaacagag
    16741 tcttatcagc agataatgat aagactctgt tctttttgtg taatttgtct gcttactttt
    16801 gatgttcgtg ttctgctaac agcaaactac cgataatccc actttcattc ggcagtcccc
    16861 aacgaacgat atattcactt acttgaggtg tttccatata accacccatc aattcaacaa
    16921 attgctgacg gattttttga aggagatgat cctgattcat taccccaccg cctaaaataa
    16981 tcttttcagg tgctaacgtc aacgtatagt tgatcagtgc ttgagcaata taataagcct
    17041 gaatagccca aacaggatga tcttgcggca gatcttgccc tttgatccct gtgcgtgcct
    17101 caatagacgg tcctgctgct aatccttcta aacaatcttt atgataagga caagtacctt
    17161 cataatggtc atctggatgg cgtttcaccc aaatatgtcc catttcagga tgtgcgatcc
    17221 ctgaaaaaat ttcttcattg atcacgacac ctgcaccgat tcctgttccc acggtaaggt
    17281 aaatacaact attttttcct tgtgctgctc cttgcttcaa ttccccatat gcagcagcat
    17341 tgacatccgt tgtccaagca gcaggaatat tgtagcgttc cttgattgtg ccaagaaaat
    17401 cgaattgtgc ccaccctttt ttgggagttg ccaaaacgta tccgtaatta tcttttgttt
    17461 tgtcgatacc aatcgggccg aatgagccaa tcccgattcc cttcaattca taacgatcaa
    17521 aaaattcaaa tacctgatcc aatgtttctt ctggtgttgt ggtagggata cttaattttt
    17581 ctaaaatttc tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc
    17641 cgccatacat tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat
    17701 tgggacaacc gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt
    17761 gtctttagtt atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac
    17821 tttatcacta aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt
    17881 taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa
    17941 aggaatctgt gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag
    18001 ttcaatgctg taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata
    18061 tccccaatcg cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc
    18121 tgttacttca tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg
    18181 aatattgaca aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac
    18241 atggtttggg cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa
    18301 ctttgtagga aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc
    18361 aaagtcgatt tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg
    18421 tttttctttg gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt
    18481 ttctaaacgc tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc
    18541 tatttccggt gtatctacta atacttttac ttcagagttt tctttgttac tggataggat
    18601 tggctgatca cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc
    18661 aaaaatatgc agattattca actttcgttt gtttttgcga tcttccattg taatggtacc
    18721 atttttttca atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat
    18781 cattgtttcc ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga
    18841 aaatgctggc atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac
    18901 acggaaacgg tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc
    18961 tggtatagtg gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa
    19021 ctcttgatat agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt
    19081 tatttctgct tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct
    19141 gtcttttggg aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc
    19201 aagatacttt ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga
    19261 atcgactgaa caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc
    19321 ataatctaat tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg
    19381 ttctgtaata ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg
    19441 agcagatgct gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc
    19501 ttctactgag cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa
    19561 attagaatga ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga
    19621 tatatctttt tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga
    19681 ggcatattgt tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc
    19741 tactggaatc tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt
    19801 ttgatcaggt cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa
    19861 aacttgatta tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc
    19921 agcttgtttc attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc
    19981 taacatgaca ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac
    20041 gttcgattcc gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa
    20101 ttttccttca ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata
    20161 gtcatctaaa ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc
    20221 aaatagctct agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc
    20281 catgtaccat tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta
    20341 cgatacctcc tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat
    20401 catatttgcc caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc
    20461 ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt
    20521 ttcagatttg tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt
    20581 ttctggcgta tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt
    20641 ttctttactc aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata
    20701 tcccaagtaa ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt
    20761 tcccattcca tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc
    20821 gtgattctga atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc
    20881 tttatcatcg agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa
    20941 catattggat ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga
    21001 ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc
    21061 ttttcttgtc gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt
    21121 gaacacgtcc aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt
    21181 tcctgtattt ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc
    21241 atattttctt tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc
    21301 gttagcttct tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt
    21361 tttgactaac ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata
    21421 aggacgaact tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa
    21481 aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc
    21541 tgtaaaattc tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat
    21601 aaatttctgc accgattttg gtatatcttt ataagccact aataaaacct tctttctatt
    21661 tgatatatct ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata
    21721 tctttttatt tactttttca tccattatgg tattatttga atatgatata tcattaagag
    21781 gtgtttatat gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt
    21841 taactggtga attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct
    21901 ataaagtaag cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta
    21961 tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg
    22021 cttctcctac aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg
    22081 ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga
    22141 tgacgacgct tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg
    22201 gattgattta ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat
    22261 tgaaagaatt ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg
    22321 ttctttccga taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac
    22381 attcaaaaat cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt
    22441 accttggtta ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg
    22501 acaaaagcgc gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt
    22561 ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt
    22621 atcatatccc tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg
    22681 ttgacccgcc actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag
    22741 atgctttggt cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt
    22801 cattcatcaa aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact
    22861 agacatgcaa cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc
    22921 gttccttttg ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg
    22981 tttcatcctg atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg
    23041 atgggtgatt tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc
    23101 cttttccatg ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg
    23161 tcgatctgtc aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga
    23221 atcttatcta ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc
    23281 catcagctct ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt
    23341 tcagaagatc ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt
    23401 gatgagttag ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca
    23461 aaaaaacaaa ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac
    23521 ctggaactag aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg
    23581 cagtcctatt ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt
    23641 tatcataatt tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt
    23701 gggcaaaaaa tccactatga tacgactagt cgatctataa aacctggtgt tctctataca
    23761 aataatgggt tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta
    23821 gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg
    23881 ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc
    23941 attgcagatg ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca
    24001 atggaaaaag gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa
    24061 gattacttga catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta
    24121 gactatgctt atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa
    24181 atgacgaaga aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca
    24241 ggatttatgc gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt
    24301 tggggacaag attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat
    24361 ttttcaggac tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag
    24421 ctgtgcaatc agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg
    24481 agtgagatgg ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac
    24541 tatccttact tgttcagtta tataggacaa cctgaaatgg cacagccact gcttaaacag
    24601 ttgatgactc agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga
    24661 agtatggccg cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc
    24721 ggtgaatatg ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga
    24781 aaggaattga cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc
    24841 aaacggatgg acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc
    24901 ggcacgatct cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat
    24961 ttgccttttt ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc
    25021 aagtaataag aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat
    25081 taccgcagat gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc agcgttctga
    25141 cctgagtatt agagagacaa atggcaaaat agttcctcat attttgccat tttttgagct
    25201 aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggtcgtgaaa aagctgttta
    25261 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    25321 cttaataccg agaaactggc ttttgaactc cgtttatcga gaggtcatga aaaagctgcc
    25381 ttgcctcaag taataagaac agcccaaaat tagatttcta gacaattttt tataagttat
    25441 ttcattacat tggctttttc ttttatgatt ctccactgtt ttctcggtac tctttagggg
    25501 atctgccgac gattttcttg aataatttag agaaatacgt ggtgttgttg tagccaatct
    25561 cacttgctat ctcattgatg ttttgattac tgtgcaaaag cagttgtttc gctttttcca
    25621 tacgtaaatg gttcaaataa gctgaaaaac tttttttcgt ttcttttttg aacagctgtc
    25681 ctagatacat gacatttaaa tgcaggtttt ctgacacttc tttgagtgta agtggttctt
    25741 gataatgctg gtgcaggatt acgattactt tttccacatt gtcactgtac tttttctgtc
    25801 tctgatgctt tttgatgaat gacagaagta gctgctgaag ttcttgcacc gtttttgcct
    25861 gatggatttt ttcgattcct tgtaaatatt cctcatcttc cagatcaatc aattcccgtt
    25921 gaatctccat aaacagtaaa aaactggaat atcgtatatc ttccggcatc atggctgcta
    25981 actgaaactg ttcaaaaaaa tcagtgatca ttttttgagc gacttccaat tgtttatttt
    26041 gcagcacgcg tctgaaactt gaaaaatcga tggtttgatc ttttgtttct gcttgtactg
    26101 cgtaaaaaac atgctcgtct ttatctccat aaaattgatg caactgtaga ttgtctttcg
    26161 cttgttggaa tgtttcaggg attttgtcca aagaatctgt ctcttccccg atactgatca
    26221 gccattcctg atcaggaaag ttttcttgaa caaaacaaca gaagccatca acttcatctg
    26281 cctccaacaa caacgtgatc aatacacggt ctccgtaatt tcgttgatag taaaagattt
    26341 gttgccgttc ttgcaaccat ctattgataa gagtatcatg agttcttgcc agttgaatca
    26401 atactatcct atgctgcttc tcacttattt gtcctattat ttcttcttca cttatttcat
    26461 ccaattcttc attcaaccat tgactgaata acagttcttg atagatttct tgttgatttt
    26521 tttgttcatt ttgctgatct aaccgtgttt tgattttctt gaggctttca accaattcga
    26581 acttattcac gggtttcatc aaataattca cggcgcctaa ttgcatacct cctttcaaat
    26641 aatcgaattc ttggtatcct gataaaatca taaattcaaa attatgacgt tctttttgag
    26701 ccgcttcaat aaattccagt ccatttattt ctggcattgt cacatccgtt actactaaat
    26761 caatctgtcg ctgttcaagt acagctagtc cttctttggc attctctgct gttgctacta
    26821 tttgaaagcc taaactttgc caatcaatta tcttttttaa cccattcaaa atcatgtatt
    26881 catcatctac caataacact tgatacacgc tcatccctcc ttgaatgtaa tagaaacggt
    26941 cagtccacct gtctcattat tctcaagcga catataataa ctgctgccga agctggcacg
    27001 taaacgttca ttcacgtttt gcagaccgat agaaccgtga agttccactt taggatgaga
    27061 cagtttttct tcaatctgtt ttagccgttt ttcagtcatc ccttttccat tatctttgat
    27121 gatgatcctt acccgtttgc cttcctgcaa gactttgaca cttagcgcat tatcgaatcg
    27181 agtaaaatca ataccgtgtt taaaataatt ttcaactaat ggctggatca caaatttagg
    27241 tacttcgatc ttttccaaat caggatcgat cataaaatga taggctactc gattaggata
    27301 acgcatttga tacaaataga catatttttc gcaaaagtcc agttcttctt ttaaagtgat
    27361 cgttttctct tgattcgtat tgtttcgaag caaagcggaa aacgcataca caacatctgc
    27421 taattcttca cttccttcgc tgattgcata cattcggata tattctaatg tattatataa
    27481 aaagtgtgga ttgatctgtg cttgaagcgc ccgcatatgt gcgtcttgct gtttgatttc
    27541 taatttgtag atgtcttcta caaactgatt gatgctatcc aacatttcgt tgataccgat
    27601 tgaaagttcc ttcagttcat attccgtgtt tgttatgtcg atccgcgtct ctaaattccc
    27661 tgctgctgtt tgatccatgg cctgcatgac gatagatact tgctgtgaat atcgtttgaa
    27721 tgttttataa agaaagataa gcagtatatt gtctaaaata atgataccaa tgatcagcgg
    27781 ggcaagattt tttcccgttt cgatttttac acttttttta tctagtacag ctaatatacg
    27841 ataatcatca gacagttctt ggtgttgaac aaaattgttt ttttccagat cgtcaatcgg
    27901 caattgtgaa tttttcttca gactctctgt gatgatcatt tcttgtcttt caaacgtttc
    27961 tttagctatt ttattatcat ggaatgtgta tagtcgattt gtcgttccag aaatcatgta
    28021 taaagagagt ccattgaatg acgttagatt ttctaaaatc atatctagtt ctttcttttg
    28081 aaaaccaata aaaatacttc ctgactcagc tcctccttgt gtgataggca acttgatata
    28141 aaaggcgtct agtaattttg gtgtcccctt ttttatttcc ccacctttgt tttctctaga
    28201 agattcataa tactccggta gttgattcaa cacgatatat atcgcactta gttcttcata
    28261 atttgcgtag agatctttga tttgatttgg gaaagaaact aattcatcgg tactgttttg
    28321 ctgttcgtat acatacatca gatactgatc tatgggtttt gttaagtagg tcgtcacatt
    28381 ttcgatcttt tcggaattcc ctacaagctg gtttttgatg atcctactca aggttgtttt
    28441 atcattcagc attctgcttg tgctttgaac ggcatcactt gtcatgtttt ctgcctgttt
    28501 ttgattttgt tcccaagtat ggatactaaa aataacggta gcagtcatcg ttacaccgat
    28561 catgataacc gcgtatttcc gcaacagacg atttagtaag gttccttttg aaaatagaat
    28621 ccgaaactta ttcataactt tccaactgtt tatctaattc ttcacgccaa tgtgttgtca
    28681 tcgtaccatt ccaaaccgtc aaaagaccaa tgacaccaaa caggatcaat cctttgaatt
    28741 gccaagtcac gcctaaaatt ataccgctac caataattat ttttagaaaa gacgaaaagc
    28801 tggaaaaaac cgaaataaaa cttagcttta gcagattagg aacagagatc gtatattcac
    28861 tatctaaaat catactgtat tgataagtag caaataggca gagcaaggca aaaagtaaaa
    28921 taaaatcaat agctaaaaaa atgagccctt tgatttgaac agataaatat aaattgtaac
    28981 tcaataaagc agtaccagat aaaaataaac taaatagcag attgcctctt aaaaaatttc
    29041 ttttatatat attccagcct ctttttattg tgatttcttt atactcgaat ccgtacaaat
    29101 aaaatagttc attgactgtt ttccatgccg cacctaatcc aaagatgatc ccgccggcaa
    29161 atgtatatat ccagaaatac aaggttagtt ttataatgac ccagatccgg ataaataact
    29221 cttccaaaac cttccctaac atatcttctc ctccaaacat taagtttata ctttctcatt
    29281 atacaaaaaa aggttgggca atcagcacct tgacctgatt attagagaaa caaacggaaa
    29341 aatagttcct catattttgt cgttttgtga gcgaataccg agggttagct gattgaacac
    29401 cgtttaccaa gagtttgtga aatagccgct tagtttcgag tagtaagaaa aattttgtga
    29461 atatggcttt ccacattttt acatatattt agcttaatac cgagaaactg gcttttgaac
    29521 accgtttatt cggaacaaga ggttgtgaca cgtcttttgt cacaacccct ttgtctttat
    29581 tcattattat tcttgtcgga actcatcata ttgtttttgc atttctttca ataccttatc
    29641 atagccagcc ttgtccaaag cgtctttcaa tttcggtaat gtttcatctg gatcaacagt
    29701 ccctgtattc aatccatcta aatattgatt catgacatta gaaatattac ttaattctgt
    29761 tttgacactg tcagtgttga agctgaatcc taaaatcggt gaagtttctg catcagcaat
    29821 cgattgatca cgtttggcaa tcatgtcatc cgtaatagac tcttgtgtat atagaatctt
    29881 gttgtttcct gtattccaag cagacatatg tgtatttggc tgataaccat ctagcaattt
    29941 gattttatca tctgaaccat cgactttttc ccatgcttct ccctcgatcc cccaaacaag
    30001 cccattcaat aattctggat cactgttaag cagccctaga agttctactg atttttcttt
    30061 attttttgaa gtatttgata cgacaaaatt tgccatttgt gcttgagctg tagattttag
    30121 tggaacagta attgcttttg agacaagttc ttgttttgct gcatttgtca aaattgtatc
    30181 gccataatca tacggtcctt gtgtttctcc acgcattagc caagtatttc cttctagtgg
    30241 ataatcctta ttgctcgttg cagcgtcact tggaatgagt ccttgtttgt accagctatg
    30301 cattgtacgt aacagtccga tatacgtttc attatcgtat tggttgatga ttttcgaatc
    30361 atctccatcc agatcaatag caaatggtaa cgtattccct aaaggataat caaagttccc
    30421 ttggactttg aagccttgtc caatggcaaa agctgccgtg tttggttctt tttcatggaa
    30481 ttccttcaag actttttctg catcagcgta agattggatt ccatcaattg aaagatcgta
    30541 tttatctagt aaagctttgt taaaagtaag tacttgttga gcaaagacat ttccattgac
    30601 tgggaaagca tataattttc cattgaccag atttccttta atatatgctt catctaaatc
    30661 attataagct ttttcagcgt acttaggcgc taattcagtc aaatcagcaa aggctccttt
    30721 ttgggcattg gaaacatagt tgttcgcaaa ggcgatatca taattttccc cggaagaaat
    30781 gatgacgttc attttctttt cgtaatcgcc ccagccaatg taattgatat tgacttttac
    30841 accgatctct ttttcgattc gtttgtttgc tacttccatc aattgatcga aattatctgg
    30901 tttatctccg atttgataca tctgcaaagt gtccgaattg ctagcttctg cctggtcttt
    30961 agaaccagaa cctccgcatg ctgctaatgt tcccaacaac aacgctgaaa cacctgtaaa
    31021 ggctagtttc ttccataact tcatttttct tcctcctaat tttatgattt atatttttgt
    31081 ttctttattc ttttactccg ccgattgtta gacctttgac aaaatgcttc tggaaaaatg
    31141 gatagctgat cgcaataggc aacgtcgaga tgactacgat cgccattctg gcagattctc
    31201 ctggaactga ggctaatccg cctgtcaatt ggcttcctgc tcctgaattt tgtgtcagat
    31261 attgaatatt actttggatt ttcatcaaaa gatactgtag tggaactagc ttatcatctt
    31321 ggatgtatag taaagcattg aaccagtcat tccagtaacc caatgcggca aataaactga
    31381 ttgtcgcaat acctggaaca gctaacggta aaacgatctg aacaaatatt cgcagttcgc
    31441 tagctccatc gatccgtgcc gattcaataa tcgaatcaga gaccgaacgt ttaaagaatg
    31501 tgcgcatcac aataatattg aagggactga cagccattgg caagatcaat gcccaaatcg
    31561 tatcttttaa ctgaagaaga tttgtcataa caagataatt ggccaccatc cctggtgaga
    31621 acaacatcgt aatcaaacaa aaaacggtaa aaaaacgacg gaatggaaag tttgaccgtg
    31681 aaatcgcata agcatataaa gaagtagccg tgctattgat cagtgtacct aaaacagtaa
    31741 caagtacagt gacgaaaagc gcttggaaaa ttttatcctg catctggtcg aacaagtacg
    31801 tataaccaaa tatactgaac ttttctggcc aaaaactgta cccgtgagtc gacaatatgg
    31861 actcatcggt aaaagagatg gcgataacaa atataaatgg caaaatacag gaaatagcaa
    31921 agagtgcaat cattatatta gagaaaaaat tagccgtctt attgaaagag cggacttgga
    31981 ctctctccac tttctttttt ttcatttttt tcctccttag aataatgcag aatcgctatc
    32041 aaaacgtcgg gcgatcgtat ttgtgatcat caacaagaca aagccgacaa ctgattggta
    32101 aagtcctgca gcagccgtca taccaaaatc tcctgtggat gtcaggccat tgtagatata
    32161 cgtatccaaa acttgagtca catcatataa tgcaccggaa ttcctaggaa tgttataaaa
    32221 caggccaaaa tcagctcgga aaatgtttcc tacggctaaa atcgttaaaa tcgtcattag
    32281 tggaataagt tgtggtatcg tcacatgttt gatctgctgc catttgctag ctccatcaac
    32341 catagcagct tcataataag tcggatcgat tcccataaca gatgcaaagt aaataatgct
    32401 gttataacca atccctttcc atgtaccaat gaacaccaag ataaacggcc aatatttcgg
    32461 ttcattatac caattgacag cttctcctcc atgtccagtg atccactgat tgaatattcc
    32521 tttgtcagga cttaaaaaag cgtaaacaaa gtaactgata atgacccaag atagaaaata
    32581 aggtagtaag gacatcgttt gataaacttt aacgagtcgt ttatttctca actcgctcat
    32641 aatgattgca aatacgactg agatcagcag attcaaaagg ataaatgcta cattatataa
    32701 aattgtattt ttagtgatga gaaatgcttg atccgaagaa aataaaaatt tgaaattttc
    32761 ccaacccacc caaggacttt ccctcaagct agccaagaac ccatctgctg aaatatgaaa
    32821 atttttgaat gctacgacat tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag
    32881 catacccggt acagccatca ggacaagtgc tttataacgc catagattca taaaaaaatg
    32941 attgaacccc tttcgttttt tcatctaaaa acccctttcg attcattcgc aacgtaatta
    33001 tagcgctttc aataatgaga ataaataaac aaagtatagg gaaaatgaaa caaagtatag
    33061 gagaggttgt gaaatcagcg ttttggcctg agtattagag aaaaaaacgg gaaaatagtt
    33121 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    33181 cagaggttgt gaattcagcg ttttggcctg agtattaggg agatcgtgtc cttactatct
    33241 aactctgagt cacaaggaac aatggggact gtaacaaaag tcttgtcaca gtcccttatt
    33301 ccgaacaaac ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaaa
    33361 gccatccgaa agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt
    33421 attcttttag ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca
    33481 tgggtcgata ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc
    33541 tttttaaata taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact
    33601 ttccttcatg caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa
    33661 tttggagttt tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata
    33721 aacggatatc ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat
    33781 gttttttagg tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc
    33841 cctctgtgtc tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt
    33901 ccaataaatc gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag
    33961 gtttatgcaa aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa
    34021 ccgcatcttc gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt
    34081 cgatcgttcc ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg
    34141 ctaaaaactt atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct
    34201 tctgattcac ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta
    34261 gtctttctgc caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg
    34321 ctactacaag aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa
    34381 aaatcatctt tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca
    34441 atgtcctgcg ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga
    34501 tcaaggtaca agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa
    34561 ataaacgaat ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat
    34621 taattgccgc cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc
    34681 ctaggaagag tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg
    34741 tataaaataa tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca
    34801 taaaaaatga aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt
    34861 tcaacgtaac agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg
    34921 caataactac catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta
    34981 actgtgcatt ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga
    35041 ctaatttgaa gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg
    35101 tatctaacaa caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg
    35161 ccgatgtata cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca
    35221 taccgataaa tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga
    35281 ctttcatgat gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa
    35341 tctgcgacag cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac
    35401 tcatacaagg aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt
    35461 ctccgccacc agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca
    35521 acaggcctaa cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat
    35581 attcattcaa tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg
    35641 tagagatcag tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag
    35701 ccttagcatt ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc
    35761 ttgaaatgat attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg
    35821 ttaattgatc taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca
    35881 aaacattact atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt
    35941 tataagaaca attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata
    36001 cagcctttta tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac
    36061 ccgttatatt tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc
    36121 acgatatagc ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta
    36181 tccaacgctc agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc
    36241 tattaggaaa atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt
    36301 tagagattgt tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa
    36361 gtcttttgtt cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta
    36421 taacgatacc gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt
    36481 tacttaccaa tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac
    36541 tattcgttct cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt
    36601 cccctgtgtc aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat
    36661 attaaaaaga gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg
    36721 agcttctctt tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt
    36781 gtttcaacaa gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt
    36841 tatgattcaa gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa
    36901 gaagaatatt tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca
    36961 tacgaaaatt cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt
    37021 gctatggctt tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca
    37081 aaaggtaaaa caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa
    37141 aaaacagcat tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc
    37201 catttgacaa cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat
    37261 ggtatgactc atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac
    37321 ggcttgactt tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc
    37381 gaacatccga catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa
    37441 acagttgttt taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta
    37501 ttccagattc caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt
    37561 tcagataaag gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc
    37621 gatactgctt atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg
    37681 atcattgccg cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa
    37741 gctcgtgtac caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg
    37801 gactatgcac ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat
    37861 ccagatggcc gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga
    37921 catgattttg gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac
    37981 ccagcatttg aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca
    38041 gatttgatcg ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa
    38101 agcgctccgc aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc
    38161 aatggtgtag atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa
    38221 taatggtatt tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt
    38281 tgtcacagtt ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc
    38341 ttttctcttt cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata
    38401 atgaatgcga tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta
    38461 ccaacaaatg ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg
    38521 acgatttcca gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa
    38581 aacggaaccg ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg
    38641 atattgaata atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc
    38701 gcgaggaatg caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca
    38761 taagaattgt tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata
    38821 ttagctaata cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg
    38881 atccacaatg catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac
    38941 tgcgtaacaa acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact
    39001 aataatccat tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg
    39061 aaagaacgag aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg
    39121 acacacatac aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct
    39181 gaagttccta aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc
    39241 tcattgatag aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac
    39301 ataaagaatg caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt
    39361 tcatctcgtt caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa
    39421 atccccatcg tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca
    39481 ttaaaaaatc cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc
    39541 gagcctacaa tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt
    39601 ttaagttgcg caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt
    39661 agttattctc ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt
    39721 ggtcttggtt gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac
    39781 cgcttacaac aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat
    39841 acaagaatgt aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct
    39901 ggaacaaaaa ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta
    39961 cttgttctcc tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta
    40021 aacttttagc tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag
    40081 tcaggtaacg tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt
    40141 tttctaattc gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat
    40201 ttttttgata caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca
    40261 gatgatcttg tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt
    40321 ctttcagaag ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca
    40381 acttctcttc tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca
    40441 gtgtccgtgc atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac
    40501 tgtttatttt ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc
    40561 tgaaattctc aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta
    40621 acctaacgat tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa
    40681 ctaaaaaaac aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg
    40741 caacggctac ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat
    40801 cactgaatat gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta
    40861 tatatccttc ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct
    40921 ttttcggttc gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct
    40981 gctttttttg ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag
    41041 attctccaac tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat
    41101 gtaaaactgg aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga
    41161 ttccttttaa agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat
    41221 ttttaccgaa ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg
    41281 cacagataat caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga
    41341 gatgcaaagc caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca
    41401 tgttgctgaa atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct
    41461 cagtcaatgg gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat
    41521 gatcgagtag tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag
    41581 acaatctttt cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt
    41641 tgtgattcga cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg
    41701 atacacctgc gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc
    41761 gcttagaaac ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt
    41821 tgatccccaa agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact
    41881 tagcttctct tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga
    41941 aaaaacggct ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag
    42001 tatccatgat aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat
    42061 gcttagaaac gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct
    42121 ttgtcaaact ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg
    42181 catcttccaa aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc
    42241 cttgcggttc tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg
    42301 tcagtctttg taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc
    42361 cgtttttttc tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata
    42421 ataacaattc agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat
    42481 atctcaacag caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt
    42541 ctcttcgatc acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa
    42601 agggatacta acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa
    42661 catcgtcgtt tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc
    42721 gccttctgta gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc
    42781 cgttgtcttt gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt
    42841 tttcataaat caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc
    42901 tattcaaaat atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat
    42961 aatttctggc accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta
    43021 tcacagtgaa tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa
    43081 tggtcgaaag aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact
    43141 attactttcc actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag
    43201 aagcaatcga aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa
    43261 agtataaatt aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg
    43321 gaatgatagc aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc
    43381 tcatctaatg attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc
    43441 caatatacat gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg
    43501 ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg
    43561 ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt
    43621 taaaaggaat aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga
    43681 ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag
    43741 aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga
    43801 tcaaaagact aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc
    43861 ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt
    43921 aaaggagaac tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga
    43981 aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt
    44041 acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta
    44101 aaataaaatg aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta
    44161 ctaatagtga gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa
    44221 agtcaacaaa acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct
    44281 tcgattcaaa taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata
    44341 atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc
    44401 gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt
    44461 gtcgtccttt ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat
    44521 ggaacatcat atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata
    44581 aaaaaacata tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg
    44641 atatgcttta tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg
    44701 attacagagg tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt
    44761 ctcttccctg ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg
    44821 aaattaatgg tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat
    44881 catatgccaa tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc
    44941 cattggaacg ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga
    45001 cataaaatca cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt
    45061 taggtatctt tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag
    45121 tcttccaaaa gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg
    45181 ttgaggataa actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa
    45241 tttacctaca caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa
    45301 agaaatagaa ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt
    45361 tctaccaaat gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga
    45421 ggcgtaacct ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt
    45481 ttctccttat tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt
    45541 atttgtagaa agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac
    45601 tgcgaaactc aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac
    45661 tacaaaggtt tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg
    45721 ttgggttcaa ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg
    45781 gtagtttcca ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt
    45841 ttattttagc tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca
    45901 cacccaatat tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct
    45961 tatttccact gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat
    46021 tctcttttgt taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg
    46081 tcgcatccaa ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg
    46141 ctaagccatc tatcgccagt tttccatctg ctcctgtggt tagacctgac tgcagaacgg
    46201 ttccattggc atcttgtaac tcaaaaacag ctccttgcag gacttctccg ctttggtcat
    46261 cgatctttgt caacacgaca ccgcctggtg taagtcgatt ttctttcgtc aattctacca
    46321 ccgctgtctg acttctttcg atctcaaact cgattggtgt cgcatccaat tcataccccg
    46381 ttggcgcttg ggtttctacc agctgataca ctcctggtgc taagccgtca atcgccagtt
    46441 ttccatctgc tcctgtggtt agacctgact gcagaacggt tccattggca tcttgtaact
    46501 caaaaacagc tccttgcagg acttccccgc tttggtcatc gatctttgtc aacacgacac
    46561 cgcctggtgt aagtcggttt gctttcgtca attctaccac cgctgtctga cttctttcga
    46621 tctcaaactc gatcggtgtc gcatctaatt cataccccgt tggcgcttga gtttctacca
    46681 gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct cctgtagtta
    46741 gaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct ccttgcagga
    46801 tttccccg
//
LOCUS       DAHEYX010000003        35670 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHEYX010000003 DAHEYX010000000
VERSION     DAHEYX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.003"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="MB219_000093"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="MB219_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCC8425836.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="MB219_000094"
     CDS             complement(2204..4390)
                     /locus_tag="MB219_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCC8425837.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="MB219_000095"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="MB219_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCC8425838.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="MB219_000096"
     CDS             complement(4612..5052)
                     /locus_tag="MB219_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCC8425839.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="MB219_000097"
     CDS             complement(5126..5665)
                     /locus_tag="MB219_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HCC8425840.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="MB219_000098"
     CDS             complement(6280..7746)
                     /locus_tag="MB219_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8425841.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="MB219_000099"
     CDS             complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="MB219_000099"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCC8425842.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10662..11021)
                     /locus_tag="MB219_000100"
     CDS             complement(10662..11021)
                     /locus_tag="MB219_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCC8425843.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11051..11392)
                     /locus_tag="MB219_000101"
     CDS             complement(11051..11392)
                     /locus_tag="MB219_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425844.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11389..12063)
                     /locus_tag="MB219_000102"
     CDS             complement(11389..12063)
                     /locus_tag="MB219_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCC8425845.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13116..14414)
                     /gene="asnS"
                     /locus_tag="MB219_000103"
     CDS             complement(13116..14414)
                     /gene="asnS"
                     /locus_tag="MB219_000103"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCC8425846.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14454..15644)
                     /locus_tag="MB219_000104"
     CDS             complement(14454..15644)
                     /locus_tag="MB219_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCC8425847.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15665..16192)
                     /locus_tag="MB219_000105"
     CDS             complement(15665..16192)
                     /locus_tag="MB219_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCC8425848.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16239..19028)
                     /locus_tag="MB219_000106"
     CDS             complement(16239..19028)
                     /locus_tag="MB219_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCC8425849.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19175..19375)
                     /locus_tag="MB219_000107"
     CDS             complement(19175..19375)
                     /locus_tag="MB219_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCC8425850.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19827..20147)
                     /locus_tag="MB219_000108"
                     /pseudo
     CDS             complement(19827..20147)
                     /locus_tag="MB219_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20650..21768)
                     /locus_tag="MB219_000109"
     CDS             complement(20650..21768)
                     /locus_tag="MB219_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425851.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            22240..23547
                     /locus_tag="MB219_000110"
     CDS             22240..23547
                     /locus_tag="MB219_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCC8425852.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            23666..24864
                     /locus_tag="MB219_000111"
                     /pseudo
     CDS             23666..24864
                     /locus_tag="MB219_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            complement(24891..26159)
                     /locus_tag="MB219_000112"
     CDS             complement(24891..26159)
                     /locus_tag="MB219_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCC8425853.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26322..26969)
                     /locus_tag="MB219_000113"
     CDS             complement(26322..26969)
                     /locus_tag="MB219_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCC8425854.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27377..27880)
                     /locus_tag="MB219_000114"
     CDS             complement(27377..27880)
                     /locus_tag="MB219_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCC8425855.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28003..28767)
                     /locus_tag="MB219_000115"
     CDS             complement(28003..28767)
                     /locus_tag="MB219_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCC8425856.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28875..29150
                     /locus_tag="MB219_000116"
     CDS             28875..29150
                     /locus_tag="MB219_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCC8425857.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29746..30696
                     /gene="yidC"
                     /locus_tag="MB219_000117"
     CDS             29746..30696
                     /gene="yidC"
                     /locus_tag="MB219_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCC8425858.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30875..31849)
                     /gene="lacD"
                     /locus_tag="MB219_000118"
     CDS             complement(30875..31849)
                     /gene="lacD"
                     /locus_tag="MB219_000118"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCC8425859.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(31846..32304)
                     /locus_tag="MB219_000119"
     CDS             complement(31846..32304)
                     /locus_tag="MB219_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8425860.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(32301..33764)
                     /locus_tag="MB219_000120"
     CDS             complement(32301..33764)
                     /locus_tag="MB219_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8425861.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33746..34696)
                     /gene="pfkB"
                     /locus_tag="MB219_000121"
     CDS             complement(33746..34696)
                     /gene="pfkB"
                     /locus_tag="MB219_000121"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCC8425862.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            34839..35588
                     /locus_tag="MB219_000122"
     CDS             34839..35588
                     /locus_tag="MB219_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCC8425863.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT        10196 a         7672 c         6227 g        11575 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
     8041 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
     8101 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
     8161 aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
     8221 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
     8281 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
     8341 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
     8401 tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct tctggaagca
     8461 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
     8521 aatcgcccgt caacatgact gtgttcttca caccagcctt ttttaattga accaatgcgt
     8581 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
     8641 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
     8701 ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct aagatccctt
     8761 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
     8821 cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaacatat tgcatgaata
     8881 tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc
     8941 cggttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt
     9001 ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta aggggtacgg
     9061 agatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt
     9121 cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc
     9181 ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt
     9241 ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag
     9301 atttttctac ttgaactaaa aatggtttat ttttattgat aaatccactt aatactgttt
     9361 caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg
     9421 attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa
     9481 actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc gtctgcacgt
     9541 gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg
     9601 ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat
     9661 attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga
     9721 tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat
     9781 aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag
     9841 cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt tcatttccat
     9901 gttcagatac tttttcctta tctatttctg ttactttgac atctggttct aattccttca
     9961 ctactttttt tatttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta
    10021 gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc tgaactcctt
    10081 tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt
    10141 taatgacctc ctcgcctcaa catatgaata tctcttcata actaattata aatgaacggt
    10201 tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct
    10261 tccatacaga tgttttggct tattctttct ttttcttttc gatcattgcc actgctctta
    10321 tccaacccgc acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg
    10381 ctggcaattt gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg
    10441 caaaatgtcc ttcccagata ttcgaagctc cactacgctt tcaaaaatat agaaagagtt
    10501 ctttaatgaa aattatttca actttccata aacagtaacc ccatataaat aaaagacgta
    10561 aaagtcacat ggaagaactt atacaggaga acgcatagag tcaggtgaaa tacatgtatc
    10621 ccacctgact ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg
    10681 aatggcatca acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg
    10741 atcgtgatac atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa
    10801 acgatcagcc agatgatatt aacaatcaat gacatgccag actggtgata aattacttct
    10861 ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata
    10921 cctatgatcg tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt
    10981 ccgccgaata taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta
    11041 ctgtttttga ttaaaactgg gcaaaaaatg attttattct tcttttttta gcttccagta
    11101 tcaaatattc tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt
    11161 gttggattag ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg
    11221 cttgcgcaaa tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct
    11281 tatcatttag gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca
    11341 gctgattgat ttgaggtaga tcttctttta ctgcccaccg tatttccatc atttctcact
    11401 tttctttcgc tcatttttat ctccactaaa aaaatcgact gcggcaattg catctatcca
    11461 gctttgtgca atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata
    11521 tttcgaaaaa ccataaacaa tccctaaagc attgaatata ccatctaaag gaaccagcaa
    11581 tgcgccatat tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg
    11641 ccagccatga cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca
    11701 gattcccact gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc
    11761 agatttctca gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc
    11821 gatcaaaatc gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg
    11881 cagatctgca attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg
    11941 atattgggag attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc
    12001 ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt
    12061 catttgtcaa aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata
    12121 agccctatca agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagtccctt
    12181 caaacaattg aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg
    12241 tgccggattc cccttgattc cttcgttgtt tggtccagag agatcgatca cacctaagct
    12301 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
    12361 ttctagtaaa tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata
    12421 actttatctg gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc
    12481 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga
    12541 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc
    12601 tttgtaaatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
    12661 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
    12721 actgcgagag ttcgcagttc ttttttagaa taaacggtgc tctccggcca actcctcggt
    12781 cacaagtcac aatagtcatc aaacatgaaa aactgttttt tgaaattact ccttgtgact
    12841 cggagctgaa cggcaggcgc acaacctctt ccttaaacgg tgttcaaaag ccagtttctc
    12901 ggtattaagc caaatttatg caaaaatgtg gaaagccatt tccacaaaat ttttcttaat
    12961 actcgaaact aaacagcttt ttcacaacct ctccactaaa cggtattcaa aagcttacat
    13021 ctgcggtaat aagtcccgcc aattcaaaaa catgaacagc tgtttttgct tggctgttct
    13081 aattacttga tgcagaacag ctttttcatc acctcttatg gataaattcg gttcaacaaa
    13141 cgtgggaatg gaatagcttc acgtacatgg tcgataccag caatccaagt gactgttcgt
    13201 tcaagaccta gaccaaaacc agagtgcgga acagaaccgt atttacgtag atcaagatac
    13261 catgaatatt ctttttcatc caaaccgtga ttacggattt gttctagcaa gaaatcatag
    13321 tctgttgccc gttcgcttcc gccaataatt tcaccgtaac cttctggtgc gatcatatct
    13381 gcacagatca ccacgtcttc tcttgttgga tgtggtttca tgtagaatgg cttgatggct
    13441 tttggatagt ttaggataaa gacaggctgt tcgaatgaat tcgcaatgaa cgtttcatga
    13501 ggtgaaccaa agtcttcgcc ccattcgatg tcatcaaatc catttttctt cagtaattcg
    13561 atcgcatcat cataagtaat acgagggaat ggcaattttg tataagtttt taagacttct
    13621 ttgtctcgtc ctagtacatc taatgcatat tcgcaatgat ctaaaacatt ttgtactaag
    13681 aatgctacat attgttcctg tacttctaaa ctttcttctt gatgagtgaa agccatttct
    13741 ggttcgatca tccagaattc gatcaaatga cgtcgtgttt ttgatttttc agcacggaaa
    13801 gtcggaccga atgaaaagac ttttccgaaa gccatagcag cagcttccat gtataattgg
    13861 ccactttgag ataaataagc tttttgatca aagtaatttg tttcgaatag atccgtagtg
    13921 ccttctggtg cagaaccagt caagatcggc ggatcaattt tgacaaaatg atgttgatta
    13981 aagaattcat acgttgcacg aatgatttca tttctgatct gcataatcgc atgttgtctt
    14041 gaagaacgca gccataaatg acgatgatcc atcaagaagt ctgttccgtg ttcttttggt
    14101 gtgatcggat aatcgtggct ctcgcttacc acttccacat ttgtaattcc gatttcataa
    14161 ccaaattttg aacgactgtc ttcacggatt tctccagtta ctaacacgga tgtttcttgg
    14221 tgtagatttt tagctgtttg gaaaacttct tccgacactt cgctttttac aactacacct
    14281 tggaaaaagg ctgttccgtc tcttaattgt aaaaatgcga tttttccgct tgatcgctta
    14341 tttgcgaccc aagcgccgat tgttactgtt tctcccacat ggtttttcga atcgatgatt
    14401 tgaatttgtt ccacgtatga ctctcctttt atattccttg aatttcttta ggattaattt
    14461 tgacttttac tctcaataaa ttgcttgatt ctgcgtaccg cttcttctaa cgtagcgcga
    14521 tcagtagcat aactcatgcg gacattttct ggtgccccaa agccttcgcc agtcacaagc
    14581 gctacccctg tttcttctaa aagatcttct acccattgtg tgacattttc atatttacac
    14641 atgcgtagcg tttcttttac attaggaaaa agataaaaag ctccttgtgg cttttcaaga
    14701 gaaacacccg gcagttctgc cacaagcgaa tataacgtat tcaggcgttc ttcaaaggcg
    14761 ttccgcattt cttccactgt atcttgctcg cctttcaatg cttcaacagc tgcatattga
    14821 ctgactgctg tcggattact ggttgactgg gaagcaacag cgatcattcc attgatgatc
    14881 acaggatttc ccaccgcata accaatgcgc cagcctgtca tcgcatatgt tttagacacg
    14941 ccgttgatga tgatagtctg atttttgatt gcttctgaaa tcgttgcaat tggagtaaac
    15001 tcattgccat tataaactaa acgaccataa atatcatcag caactatcaa aatatcattt
    15061 tttactgccc attcaccgat tgcctgcagt tcattcttgc tataaatcat tccagtcgga
    15121 ttcgatggag aattgatgat tacggctttt gttttttctg aacgtgcttg ttctagctgt
    15181 tcaacagtaa ctttccagct attggattct tcccctttca caaagacagg aagtccttct
    15241 gccagtttta cttgctcacc gtaactgacc cagtaaggca caggaatgat gacttcatct
    15301 tgcgggtcaa ggatcgtttg gaaaagggta tacaaagcaa atttcgctcc gtctgttacg
    15361 actgtttgtg aaggttcata gacaagtcca tagtttttct tcagataatc aacgattgcc
    15421 tgtcgaagct ctgggattcc tgctgttggt gtataatagc tagctttccc attgcggatt
    15481 gtttcgattg ctgcttcttg gatgttttct ggtgtcgcaa aatccggttc tcccacagtc
    15541 aaactcagca catctctgcc ttttgctttt aatgccttgg cctttgccgc agctgccaaa
    15601 gtgacagacg gttctaatcg ttttgttctt tttgataact ccatcccatt cattccttta
    15661 ccttttaaat tcccttgatt gtcttgactt catctccaga ttcaaagcca atcaggtaat
    15721 aattcagttt cccatttgtt tcttttccta ctacttccca cacaggctga tcatcataca
    15781 tccctaaagc agctttttcg atttggattt caggatgggc cgcagcaatt gtctgttttg
    15841 cttcgttttc agtcagtccc tttgtttgat ccaatacttt tactttttca ccagatttag
    15901 gaatgataac ggcgatttct tttcctttat catttgtacc tgtcagtgaa aagtatgtct
    15961 tctttcgagt aaaccaataa aaatgatcgg tttcctcaag atttgcatat ttctttgcga
    16021 tttctactgc ttctttcttc gcctgcaccg ccggacgact ggaacgcaag taaaaaatcg
    16081 agctgatcac gatgaccgct aataaaaata cgatcaagcc aagtaacacg cgattaatcg
    16141 ttttttcctt tgtctgtttt ttcttcaagg gtcgtcgctc ctttatcttc attccttttc
    16201 attatagcag attttaatta tgaaaaaaga cagctcctct aacttccttc ttcatttttc
    16261 tttaagaatt catggatatc ctgatagatt tcctccatag ctagctcttt gatcggtact
    16321 tttttcggta atgcatggat gatccgtgcg ccatattttg cagtgactag tcttcgatcc
    16381 aaaataagca atacgccacg atctgtctcc gtccttatca agcgacctaa tgcttggcgt
    16441 aacttcaaag ctgctttagg tacggattcc tggtaaaaag gattgatccc ctctgattct
    16501 aaataagcgt tcctagcttt gaccatcggg cgaagcggat tatcgaaggg taaacgtgtg
    16561 actacaacga tctgcaaagc atctccaggc aaatctacac cttcccaaaa gctgtcagcg
    16621 ccaaacaata cgcttttttc tgacaaaatg aagcgtttca aaagcttctc ccggcttcca
    16681 ccaaaccctt gagctagtat ttctcgtcct ttttctagca tagacagatg cattttctga
    16741 tagaccttct gcaaaacatc atgagaagtg aataacacaa gtattggccg atcttcttgt
    16801 tcgatcagac ctgtaagtgc tgtctctaaa taatcgatat atgcttgatt gctgacttga
    16861 tggacggcag gtgcctcttt tggcacatag atccttgcct gtttctcata atcataagga
    16921 gaggcaatca ctttaagagg tgctgcttca atgccaagcc tcttagagag atagtgtcga
    16981 ttagggccga tcttcagcgt tcctccaata taaagaatac gatcatatcg aggataccaa
    17041 acagtcgacg gcaacatagc cgcttgaaaa tcatggagat gaaaaatcat ctgctgcgct
    17101 gtgttcccag gaacgaacca atggatcaaa tgatctcgcc agtcttccag ccattgatcc
    17161 atcagcaagt attgcttgtc taattgatca aataggctat ataaacttgc taaatcgatc
    17221 cgcatctggt ttgtccagct ttcttggata atctcaaaag tcagacgcat ttgtgactgt
    17281 agctgctgga tctcctgata aaaaagctgc aggtgatcaa tggctgtccc agattccagt
    17341 ggcagctgat ccaacatctc ttttgtcatc atgacttctt ctggatactg tccgttaggt
    17401 tgcttgccgc tcactaaact cattaatcct tctgccatac aaatctgtgc ttcgcatagc
    17461 gcagaaagtt cttctcgata aatcgataac cagcgtaagg cttcatgttc tttcggtaaa
    17521 tagctttgta ttcggtcaaa cagctgattt ggttcttcta attgctggat ttgtttatgg
    17581 aaatagttaa tacttaatcg tttgtcattt actttttcag cgatttccgg caaatgatgt
    17641 acttcatcga tcaacaaaaa tggactagct ggaagcaatg gttcttttcg ctgtgtttct
    17701 tgtactaaaa acgcatgatt gacaatcaaa aaattacttt gcctgatttt cttttgcaag
    17761 aaacgaacaa agtcttgttc gtaaaatgac tgatttcttg ctaaaaatcc aatacctctg
    17821 tgcgcgatat ctgcaaagaa tgaatggttc aaatttgtta aatgtaattc atccaaatca
    17881 ccagtcttcg tttgcgtcag ccaaactaag accgccattt gatacaaagc atattgtttt
    17941 tgttctggcg gttccacaag tgtgccttta aaacgctgca aatcaatata gtggcgatag
    18001 cttttgataa ttacaggttg tatgggctga tctagcaatt gattcagcaa tggaatgtct
    18061 ttttctatga tctgctgctg caaaactagc gaaacggtac tgatgacagc gggtttttca
    18121 ggtgtagcca aataactcaa tggcagcaga taaccgattg ttttacccat tcctgtagct
    18181 gcttcgatca tcaaatcttt tgattcatct tttgtgaaat ggtcgtagac cgcattcatc
    18241 agtctgttct gttcttttcg atacatcagt gttttaccaa acattttttc ttttgcttgt
    18301 ttttttcttg gatatgtccg ctcgccataa tggacggatg tgaatagttc cacttctttt
    18361 ttctgcaaag ctagcccatc gacgatttct aatgagggat caagtggctc tggtttttct
    18421 ttcatttctt caatcacatg ataaatgaaa cgactcgtat ccatacctgt gtgtctactt
    18481 aatcgcgcaa tctgctctaa cgtgatgatt ggcaattttc tcatccgttc ttcgatcagc
    18541 aaaagaagtt gacctgtcac ttcggcatca ctatctgcct gatgaggatt ctcatgaata
    18601 aatccaaggc tttctgacaa atcagctaag cgaaagcttg gttccgtcgg caagaaaatt
    18661 tgtgcaagtt ctactgtatc gatgcctggt atcttcaaac taggaagtcc gcatcgcatg
    18721 agttcatggt tcaaaaaatt ataatcaaaa taaatattat gcgcgacaaa tatggtgtct
    18781 gccaaaagat tgtaaatcgt ttgggcgaca tcttcgaaat aaggcgcttt ttggacgcgt
    18841 tgattcgtga tccctgttaa atgctggatt tgttttgaaa tcttccgccc tggatgaata
    18901 tcgatcgaaa aacgtgaagt gatccgacct gattcaatca tgacgcagcc aaattggatg
    18961 atccgatctt cttttggatt cgtcccagtc gtttcgatat caacgactgc ataagttctt
    19021 ggtttcaaaa tatcttcctc ttctcctgcc tctaatgttc ttttgaaatt atactacaaa
    19081 gtgcaaaaaa atactctcat cgctttttaa agaaaataga caaaaacgcc cagacacgca
    19141 aatgtctggg cgtttatttt ttagtatagg ttaattaagc tttagtaacg tttgttgctt
    19201 gaggtccgcg ttggccttct tcaacatcga aagttactgc ttggccttct tctaaagttt
    19261 tgaatccgtc gccttggata gctgagaaat gaacgaatac atcgtttccg ttttctgcag
    19321 tgatgaatcc aaatcctttt tctgagttaa accattttac tgtacctgtt tccataataa
    19381 aaatcctcct tgtgtatcaa cacatatttt gcaattactt gaatggtgta tatctggaag
    19441 atgtttattt gaaacaagct accacagatt accgaacaaa aactactcct atagtatatc
    19501 ataccgttta caaaaaagct agagtttttc gacttttttt attaaaaaaa gttttctcag
    19561 cgattacata aagtgaagaa ccgaactttt tttaaaataa acgacatatt gttcagttgg
    19621 tgtttttttg ctctgatgtt cttctttaaa ctaaaaaaat tattctttct gactacttat
    19681 cggttctgtt tcaacggtcg aggctgggga agattcttta gtaggtctga tgttctcatg
    19741 gtatttctga tagcctatga cagtgaccag aagaacaagg caaaccaata cgatcaacga
    19801 gattagtttt tcttttttgc tcatatctat acctcataaa taacaggatg atgtccatca
    19861 tatttctgag tatgagtaaa agcaaaagct gcagctgtag aatgtaagaa atgccagcaa
    19921 ctttcttaat cacaggtcgg acagtaccaa aagtaaccaa atcttggctg caaagaccgt
    19981 tcttctgttt caggaatcgc agaatcaaga tttggtgtct gtacataaac atttttgtac
    20041 agacgtgtgc ctgtaatagg agaccgctgt gtttcttcag caaaaactgg ctgacttgtt
    20101 aaacttaggg atagaaacag caaacaccat aacagtcttt ttttcataaa gacttcctta
    20161 tcgataagtt tttcccaatt aaaatcatat ctcaagaacg cccgttattc acattttgtt
    20221 cataaatgat gttttttgta tcataatcgt caaaaagagg tcgtgaccac gtcacaacct
    20281 cttttttaaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa
    20341 tttgagaaat aatttttgtc aatctcttct tatttcttga agctgaccac ttctgtcaca
    20401 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc accaaacaaa
    20461 aaatatgaag agctattttt gcttgctgtt cttattactt gatgcagaac agctttttca
    20521 caacctctca tacacggtgt ttaatcagct gaccttcggt attagctcaa aaaacggcaa
    20581 aatatgtgaa actatttttc cgtttgtttc tctaatactc aggtcaaaac actgatttca
    20641 caacctcttt taataacgaa aaatcgctgt cagactcttt tcatctggcg catctttttg
    20701 atccaaaata aacacttttc cgccattaat gatcacatca tctgctaatg cattcaacac
    20761 ttgacgccga tcatattctg tttcaggatc ttcaccgaaa ccatcagcta agtttgacgt
    20821 agcgataaat aaatgtgaca cttttccttc tttcgcagct ggaacgatat caactaactg
    20881 atcgatgaat ttcttatcta agagtttatt gtattctttt gattcttgag ctgcgagttc
    20941 acttgacagc ttcttcgccc cttcttcgat atctctaaag gatagctgcg caggagaagc
    21001 agaaatcgca acgtctgcac aataataggg atttttagca atttttttga acatcgtctg
    21061 attttctggt agagcaaata aataaatcgg ccattgttct ggattttcga aagaatcctt
    21121 caagtacgta tcaactgctt ggtaatagtt gacccaatcg atctctactt cttcatcttt
    21181 tggatttacg ccatggaaag ttactccatc tcgggctccg cctcctccat tagaagagaa
    21241 gttcaaaccg ccacctgtca gttcagatcc caatgcagta acgacatcaa ccggtgcccc
    21301 ttctggtaga gatacttctt ttacttgttt gttgctgact tcatacaatt tcatggagtc
    21361 gcggttcaat cccaataaat aataagaata gttgaactgc ccatttttga taatagccag
    21421 taaataagga agatcactta catagtattg atcgtctacc gggatactca aacgatggat
    21481 ataggtttgg tcagcagtta taatgaccgc tacactcgta gttgcactac gccaaaacga
    21541 aggatctcct aacaaatttt caattttttc ttggaacggg gaccagtcat gttcgtaata
    21601 ttttttctca aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga tctgatcttt
    21661 ttccacatct tgatgggcga catgcgtatt cagcatgatc gtgacgaatg gtcctttagc
    21721 gccttctgaa gtcaattgtg acaataattc tttatccttt tttgccataa acgtacccct
    21781 cctaaaaaaa tcattccttt gtttctatct aaagtctacc acaacactat ttttccatac
    21841 aataaaacgt atctaaaaaa ataaaacggt ttctacggtt ttaaatttag ctattttttt
    21901 aagttatctt cataatttcc tcaattattt agccccagtc ttcaactatt cttcatgaaa
    21961 tgctgataaa ctaagaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
    22021 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
    22081 aaacacagcc tattcctgta aaagtacgta aaaacgaccg ctaaccttcc ataacgtgcg
    22141 ttcttatcct aaagtttttt cagaaacatt gaaactctct cccctctctg ataaaatttt
    22201 aatttgtagc gaaacaaatt aattacaagg agatcggata tgtccataaa aaaaacacgt
    22261 ctagtcctat tattggctag tctcattctt tgccttgctt cttttctacc agctgttgct
    22321 gtgcaagctg aagatacgtt caaagtaaat gcaaaagcag cttttgctgt agatgcgcaa
    22381 agcggtaaga ttctttatga tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca
    22441 aaaatcatcg gtctttacat cgttcttgat caagtaaaag aaggaaaact atcatgggat
    22501 gataaagttt cgatttcaga ttatgcagaa aatttgagta tcaccccaga tttatcgaat
    22561 gttcctttgc ataaagaaaa cacctacact gtcaaagaat tattcgactc tgcaatcatc
    22621 cagtcagcca atgcttcaat ggtggccttg gcagaaaaaa tctctggcag cgaatctaaa
    22681 ttcactgaac gaatgaaaga acaattgaaa gattggggaa tcaaagatgc aacgatcgtt
    22741 aatgcatcag gactgaataa ctcttatctt ggcgaaaacc gaccagaagg tactggagaa
    22801 aacgacgaaa atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca tttgatcttg
    22861 gattttccag aaatactgga cgtatctagc actacgacac aaatgtttgg cgaaaacacg
    22921 caatcaccag tggaaatggt caactggaac tggatgctcc caggattcgt taattacaag
    22981 gaaggcgtgg acggattaaa aactggtacg actgagcttg caggtgcttg tttcgttggt
    23041 acgatcacta aaaacgatca acggatcatc actgtcgtat tgaatgcgga cggccatgcg
    23101 gaaaacccga gtgcccgctt caatgaaaca gaccgtttga tggactactg ctatgacaat
    23161 tggtcagaaa aagagctcgg aaaagctaac gcaagtattc ctagcctaaa aaccattgat
    23221 gtaaaagacg gaaaagaatc aacagttcct gttgtgctta aaagccctgt aaaagtatgg
    23281 gtacgaaacg atatggatac tggcaaatta acgatcacgc caacacttga caagaagcaa
    23341 gtcactgaca acgagatcca agcacctatg gataaaggta caaaaatcgg taccgctacg
    23401 atcaccttgg cagacgataa attaggctat ttagaagaca acaatgcgcc atcaacagag
    23461 atcattacgg ataaaacagt ggaaaaagcg aatatgttcg tgattggctg gcgacgcgtc
    23521 gcagaatttt tcggcaactt gttttaattg aacaataaga aaagcatctt tccatcggaa
    23581 agatgttttt ttgcttttac tagtttaaat aacagctttt ttcatctatt ttcgttatac
    23641 tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgatct ttttagttct
    23701 ctatcttatt ggggtattct ctatcgttta ttggcgaaaa cagaagaagg atccttcctt
    23761 gttcttttac ggcagtacgt ggacatttac ggtaatctta acttattttg tcgtgttgga
    23821 aattttttat tcgacctttt attttttgat tccattgttg ttctttagta tctttgcttt
    23881 ttcttatttc acagaaaaac gaaggctgct gaatggtctt ttattcaatg ttttcctcat
    23941 cagtttcggc atctacttgt ttgtcttgct gtatgaaacg caaaatattt tccttggcgg
    24001 actgatcgcc ttgatcacga ttccattgct acttgtcctt ttatttggta tttatggatt
    24061 gattgttttt cttttttgga acggggtcac agttttacgt agagaatcgc attcattagc
    24121 taatctttta acgttgatac tagccatttt tcttacactg tttcttgttt ttgacttttt
    24181 cttgttaaaa tatttgccgc aatggataaa tgctttgttt ttctgcgttc ccctgatctt
    24241 gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa
    24301 ccggcctcga tacaatcaag acttcatcgt tgtgttaggc gctgggttga tcaatggaga
    24361 aacagtttct cctcttctag ccaagcgaat caataaggca attgcttttt atcgggctca
    24421 aagccgtgct acattaaatc cgcccatctt attgatgtct ggcgggcaag gagcagacga
    24481 aaaagtacca gaggccattg cgatgaaaca atatgccatg gaacaaggaa ttcccgaaag
    24541 agacattctt gtcgaaacca attcgacaac cactttggag aacatgctct attcaaagga
    24601 aatcatggat cagcaaatga aagggccata ccgcgcgatc ttcagttcca acaattacca
    24661 catttttaga gccggtcttt atgcgcgaca agccaaattg aaagcaaatg gtattggtgc
    24721 aaaaacagct ttctactact tgcctaatgc gttttgagag aatacatcgc catcttgatg
    24781 atgaacaaaa aaagacatat gatcatttgc ggattgcttt tcgcaggctc cgcattctta
    24841 tcgctgatta ctttactttt ctaaaaaaac aaggtgtgac aaaacatatg tcacaccttg
    24901 tttttttctt tcattctctt aactattggc aaaaagagta tccctttttt gaccgctact
    24961 tgctgagcag aataaatacc gctgttgcct gaacacataa agctgatcaa gcagatcata
    25021 aagaagtata ctgccgcttg tgaaccgaac aattcgatcc ccatcacaaa gcaggcaatc
    25081 ggtgtatttg tcgcaccaga aaatactccg ataaagccca atcctgcaag aaaaggaaca
    25141 gaaacatgga gcaaaggcgc tagtgctgca ccaagcgttg caccaatttc aaacaatggt
    25201 gtcacttctc ccccttgata tcccgcgcct aaagacaaaa ctgtgaagaa taacttccca
    25261 ataaaatcat agacatgtgc ctgtccatta aatgattctt gaagtaacgg aagactcaat
    25321 ccgagataac gttgtgtttg caaaatcaat gctgctgcca caactacagc tccacctatc
    25381 gtatttcgcc aaacaggatg aggaatccat tgtgcatacc gtgcttttaa aaagacgatc
    25441 gaacggctga atacccagcc aatcaaacca aaagcaatcc cagcaacaaa aactttgaag
    25501 aacagcaaca tactccattc agggatcaca cccatttggt aatgtaggtg agaaacacca
    25561 tagctttctg tcataaaatt tgcaaacagt gcagcaaaga agctagggaa gatcgcttct
    25621 gttcggactt tcccgattgc caacacttct aatccaaata atgttccagc taaaggtgtg
    25681 ccaaatacag aactaaaacc cgcactgatt ccactgataa tcaagatttc ccgttctgct
    25741 ttatccaatc ggaacaaacg tgctgtatta tctgctaacg ctccccccat ttggactgct
    25801 gttccttcac ggccaacaga tcccccaaat aaatgggtag ttatggttcc aaacagtgta
    25861 agtggaataa gtcgcaacgg gattttttct tctccaccat ttccttgatc gatcactaag
    25921 ttgtttccac gactagaaag accgccgtga tacgcataga gataagcaaa aagcgcacca
    25981 ctaactggta gtagataaag gagccaagga tgtgatagtc ggatatctgt cacaactgtc
    26041 aggctattta agaaaaaagc agacaagctc cccatgaata taccgagaat cgctgtgatc
    26101 accagccact tcagcatgta cactaaaatg cgtactgtac tcggtaattg tttttccata
    26161 aaaaattcct cctcaataaa aaaacgccta ctaaagaaag gggctgtccc cagactttag
    26221 taggcgtcat tagctatctt tcgttagcat ttaaaggcga acaccatcac ctattcactt
    26281 ccattaaaag atagcatatt tttctgtaaa aaacaataaa attatcgttc tttctttaca
    26341 cgttcagcaa attcatagaa aggagccaat ttcgttaact gttgattaca ttggtcacta
    26401 tcatgacaaa tataatttcc ctttttcgta tacgtgccat cagaacctgc tttcgttgta
    26461 gaaagaaaaa gcgaaacatt cgatgttttc tgacagattg cacagattcc cttatttatc
    26521 gttggtgata aagtaccgct aatccctttt aatttttcat cttcgtaata gaccatgaat
    26581 tttttctgcg tacctggatc attccagccg atgtaagtat gttcttgcaa gtcgatttca
    26641 ttccaagctg gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg
    26701 ctaggctgca taaacggctg aacgatttgc ttcagctcct ccaatgctct ttctgcttgc
    26761 gcttttgtca gttttacgtc taaaactgtc gttaaaaatg tatcgatttc tggcagttcg
    26821 tgatctaata tgtctcgaat cttttcagct gctagtgcct gaactgtttg taccgttttt
    26881 ggatcattga cagatgagta cgcatttaat aactgttcta cttctctttt tataaaaaaa
    26941 tattggtatg gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg
    27001 catactttgt agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact
    27061 cttttaaata agatacgttt tgcatcttat catcaccccc tttaatgctt attattctat
    27121 gtacatgatt cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa
    27181 acagaaaaaa ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt
    27241 tcaaaagctt gcatctgcgg taataagccg aaacttcaca aaaatttgag aagtaatttt
    27301 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca atatggtcta
    27361 ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac
    27421 gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg tccacgaatg
    27481 tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac atgtgcttca
    27541 aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc tttacagcaa
    27601 ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt
    27661 agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt
    27721 ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa
    27781 catgctgtgt gtaatttgct aattttttca ctgcttctgt ttctaaaaga tcttgtacat
    27841 aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt
    27901 tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc ggattattct
    27961 agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag aaaaatgata
    28021 aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt tcattgggat
    28081 atatacgttc tgatcagtca aagacaagat ttcctgactt actccctgac cttcgtttcc
    28141 catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg cttcttcatt
    28201 taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca cttctgccaa
    28261 atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca gcacctttgt
    28321 actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa acgcatctgc
    28381 cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta aaagccagcc
    28441 tcctgaaaaa tctggcgtat ctttagatgt taacgtcatc actgctaaga tcccttgcgg
    28501 tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag tagaaatctc
    28561 tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca caaaaagttc
    28621 ttttatttct acatcagacg acgcagcttc ttcgatcaga tggaaccctt ccaaaagata
    28681 ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt tgattcttgg
    28741 attttttgct gattggatct ctttcacatt ccttcacctc acctgattat atcatggaat
    28801 aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttgatttata aaacaggaaa
    28861 gaaaggtgac gaacatgcgc aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg
    28921 gatttcgcta tatgaccaag atggttgctg atcagttagg cgtgaccgga acggtaaaaa
    28981 acgaagacga cggctcagtc acaatcgaag caatcggaaa tgacgacatc atccaaaaat
    29041 ttatcgaaga agtcaagaag tctccgagcc caagtggacg agtgcagtat gtggatgtcc
    29101 aagaggatcc gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa
    29161 aggtcgctga gttttgagta ttaaggaaaa ttttgtgaaa atagctttcc acatttttgc
    29221 ataaatttga cttaataccg agaaactgac ttttgaacac cgtcgatcag gttgcgaaaa
    29281 agctgatctg catcaagcat taaggactgg ttgtgaaaaa gtcgcttagt tttgagtatt
    29341 aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccaag
    29401 aaattgactt ttgagcaccg tcgatcaggt tgtttttgag gcgctttgcc tcaagtatta
    29461 aagataggta gtaaaaaagc tgatctataa cttgcaataa atttgcttaa cccatatttt
    29521 ccagacagaa caaaaaagtt tttagttttt taaataagaa agaaacactt ggaatcactc
    29581 ttttctttcc aagtatttct ttcttgattt tttgtctttt ttcaaaattt ttcgctacga
    29641 aaaagaaaga atatttatat gaaatttcta tgaagttgat tgaattccat gcgctttttt
    29701 agtatagtta agtttgtgta aaaattatag aagaggaagt attctatgaa taaatggaaa
    29761 aactggttat tgggatccgg acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg
    29821 atggattcaa acggaaaccc tgacacttca ggaatcgttt atcgagtact cgtccatcct
    29881 atgggccaag cgattacata tctagttgag aatttcaatt ggtcttacgg ctgggcagtt
    29941 attgcaatga ctgtgatcgt tcgtatcatc atcttgccac taggtatcag tcaatcgaaa
    30001 aaaacaatga tccaatctga aaaaatgcaa gcgttaaaac cacaagttga agcagcacag
    30061 caaaagctaa aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag
    30121 caagtatatc gcgaaaacgg attgagtatg accggcggaa tcggctgtct gcctctattg
    30181 atccaaatgc cgatcttctc tgccttgtat tttacggctc gctacacaga aggtatccgt
    30241 gaatcatctt tttacggcat cgatctaggc tcaccaagta tggtgcttgt ggccatcgca
    30301 ggggtcgctt atctgcttca aggatatatt tcaacaatcg gtattcctga agaacagaaa
    30361 aagacgatgc gtacgatgct gatcgtctct cctgctatga tcgtcttcat gtcgatcagt
    30421 gcgccagccg gtgttacact ttactgggta gtcggtggtg tgttcagctg tctgcaaaca
    30481 ttcatcacaa acgttatgat gaaaccacgt ttgaaagcac aagtggcaga agagttgaaa
    30541 caaaacccgc caaaacaagt cgtcactcca agaaaagacg tgacagaatc agcaaagcca
    30601 gctgacaaca aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac aaataacaaa
    30661 ggccgcaacg caggaaagca gcagaagcgg aagtagatag taaataagag tgtaataaaa
    30721 attcattaac agttttagta attaaaaata tcgaccaaga tttttgaaaa agaaatctgg
    30781 tcgatatttt tatttaagtg aatgtttaat ttgaatcatc gattgatcgc tatctaattg
    30841 cagcagcatc tttttatgaa gaaacaactt tttcttatct atcaacgtag acaggtgttg
    30901 ctgtttcttc taatagtgta cttaattctt taatattttg ttttccttct tctaacatcc
    30961 aactactcgc tttttcttcg ccttcggtaa caaaaggtgc tactcccttc gcccatgttg
    31021 ctcgtccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa cgcaatgtat
    31081 cttgaaatag tgcagcgctg actcccgcac taaaaaaaat aaacggttgt tcagttactt
    31141 tcgattgttc atagaaataa gtggatgcct ctgcttgact ataacaagtc tcttgcccat
    31201 atcctttcac aaatctcata ttcactggta cttctacttt caatacatcc acttgataac
    31261 gttcttttga aaattctcgc atggcttcaa tcactttatg cggttttact tttgcatagg
    31321 caagagaaga cggatcgcta atagttgcat cataagaaag gatctctaaa aagaaaggca
    31381 taccttctgc ttcgcattct gaatcaatcc gttctataac tatttgtttt tgcacattaa
    31441 tctctactga ttcatcaacg tcatagtaca ataaaaattt acaagcatcc gcacccactt
    31501 cttttaacct tttcactgac caactattca gtaaatctgg tagtcggccc ggcattttcg
    31561 catcatagcc ggttttttca taagctagca acaagccaca ttctttctct cgaagcgctg
    31621 ctgcaggcag accatattct ggatcgagta aaatagcgga agcatacgga gtcaggtact
    31681 gagaaaccat ctgtttaaat tggatgatat cttctgatgt agcttcttta tattggttga
    31741 tcattttttt caaagcacca cgctggtcga ttgctaacgc gctaataatt ccagcttcat
    31801 ccgtcaatcg cttcaaacat tcctgttttt ctatactcat tttcttcata tatcttcacc
    31861 tatttcttta gttaatattg tggctatttc tgtttttgat tttgctgctc tcaactgttc
    31921 tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca aatgagtcgt
    31981 tcctgcttct gaatctggga taaataacgc aaaaataaaa tcaactgact gtctatctaa
    32041 actgttccac tcaatccctt tgttcagtgt gacgataatc aaactaggtt cgttgattgt
    32101 ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtac cttctttttc
    32161 tctttctaga agttttcgat atacttcttt ttcgtcatga gcgattccta aacagaatgt
    32221 ttgctttgct aaaaacttaa atgcttcttc ttgactatct aatggctgtt gtaaaaaaat
    32281 gttctcttca tgaatgattg tcatgataag cctcctgctc ttccttcttc gataactgta
    32341 cgatttgctg ctttattatc catcttcttg tctttatcag ttactctttt cttttggatc
    32401 aaacctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac ccattggatt
    32461 tttcccgtca ctacaggtaa cactaaaaaa ccgccatgtg gggctggtac ttggacacca
    32521 aacagatagg tcaagacagc agcaattgat gagcctaaca tcatcgttgg taatactcgg
    32581 atcgggtctt tcgcagcaaa cggaatagct ccctccgtaa tatgagttga tcctaaaata
    32641 aaattaacca aacctgcatt acgatcatta gtatcaaaat atttaccaaa caataaagtt
    32701 gcaaaaccag taatcaacgg tggtgcaatg caagctactg acacgccagc cataaaagtc
    32761 gtgtttcctt gtgccaaaag agctgtacct gttacatatg ccgccttatt aatcggtcct
    32821 cccatatcaa acgcacacat gcaaccaaca atcacgccaa gaacaagtgg attcgaatgt
    32881 tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg acccgccagc
    32941 aaagacatca cgatgccgat aaacactaca ccaataactg gatataagaa gattgccttc
    33001 aagccatcta gttgtttagg taaaattgtc agtagctttt gcaaaaataa gataaaataa
    33061 cctgctaaga acccagctaa aatacctcct aaaaatccag taccgccact catggccacg
    33121 attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc tgcaatataa
    33181 gctgctaaaa ctggaaccat catccccatt gctgcatcgc caaccgtttt taacatcgcc
    33241 gctgtagcat tgtattgtgc atggtctgga tcaaaagaat agattcccca aacggcaaat
    33301 gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga gacgccattc
    33361 attaaatttt tatagatgct gcgaccaaaa ttagcaccac ttatcgtttc tgattctaaa
    33421 gtatcaacag cttcattatc tgctaagatg gtcccttttc ctgccaatgc ttcgtcaatc
    33481 agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg ttttcccgca
    33541 aatctttctg aatggacatc tttgtctgcg gcaataatga cagcatctgc ctgtttgatt
    33601 tcttcagccg ttaattcatt ctcgatccca atttgtccat gggtctccac tttgatcgtg
    33661 atgccttttt ttctagctgc ttgctctaat ccctcttgcg ccatgtaggt gtgtgcaatt
    33721 cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatctttcg aacctctctt
    33781 ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc agtcctttac
    33841 gaaaagcagt agagctgcct gctgctatgc ttttacgtaa cgtttcatca agcggtcgtc
    33901 gattcatgta tcctactaaa aaagtgccta acatggcatc acctgcacat gctgtattga
    33961 caacttttcc ttttggcgag tttcctcgaa tcaccctgtc tttcgtaaaa agaatcgctc
    34021 ctttctcacc aagggataac aaaacatttt ctgcacctga ctctaccagt cgttgtccat
    34081 aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa agttcttcct
    34141 cattcggttt cagcaaaaat ggtcgatagg gcaaacaacc catgacctct tgatcactgc
    34201 tatcaataat caaatgaatc tccttttcct gacaaatccg actgatttcc actaaaacct
    34261 tcggagacat tcctcttggt aaactcccag aaacacaaag ataatcctct gcttgtaatg
    34321 ctcttatttg atttaaaaaa ttatggacag ctgcctgcgg tatttcaggt ccttgattca
    34381 ctaatttgta ttcttctctc gtttgattga cttgtgtaaa tacattgata cgcgtcatac
    34441 caggcacttc aataaactct gtttggattt gtttcttctg taaatagtct tctatatatt
    34501 ttcctgtgaa accagcagaa aaacctaatg ccgtacttgg gaaatctagc atctttaata
    34561 ctagtgacac atttacccct tttccgtttg cctgaatgtc atcctccttt gtacggttta
    34621 ccataaaagg atgcatctcc tctgtttcaa taaatagatc aattgccaaa ttcatcgtgc
    34681 atgtgtagat agacataacc aatgacctcc ttgaatctat tatgaaatac gcgaaagaga
    34741 aaatgttttc ttaatcgttc caacacaaag aaaaccttta catttataaa cacatcgtgc
    34801 tattttttaa ctaactttta gtgaaagaag gaaagaatat ggatcacaaa ttaagtgaaa
    34861 cagaacagta cctctggaac tttatcgaac accacatcct agagatccct aactactcta
    34921 tcgtgaaact aagtgaacaa gcaaatgtct caacagccac cattgtgcgg acaatgaaga
    34981 aaaaaggcta tgaaggattt acttcgttta agcaccatct aaaagaagga gaaaacaaga
    35041 acatcaattt ttctttctta gataaagtag acaaaggtat ccgtcgagcc attctaaaaa
    35101 atgaacaaga agtggtacgt acgatcaata tgttggaaat cgggaacatt gaagatgcaa
    35161 tacaaagaat caaatttact gaccgggttt ttgtctttgc ccgaggattt tcagaaatga
    35221 ttggtcaaga gatgctcgta aaacttcaat taacaggaaa aaattgtcaa atgcatactg
    35281 atccagagat tatcaaaagc atcagtcgga aactaactaa gaaagacagc gttatttttg
    35341 tttctttgaa cggcgagaca aaagaactcg tcaccgccgc aaagaactgc tatgatgcag
    35401 agattggttc gattctagtt acagccaatc attcctctac actcaaagat tattgcgaga
    35461 taaatctcgt aggtttcaaa tcagaaggtt cttatttccc tgattatgaa gtacgttcac
    35521 gcttgcctct ccagatcatc gcgagaatcc tgcttgacgc ctatgtattg cgtatgaggg
    35581 agaattgaga agcagtttat tatggttggt tacagtttaa aacatcacat tttttattct
    35641 ttatattgta tggactcacg taaacttttt
//
LOCUS       DAHEYX010000004        33171 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHEYX010000004 DAHEYX010000000
VERSION     DAHEYX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.004"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            100..3267
                     /locus_tag="MB219_000123"
     CDS             100..3267
                     /locus_tag="MB219_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425864.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3287..3832
                     /locus_tag="MB219_000124"
     CDS             3287..3832
                     /locus_tag="MB219_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCC8425865.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            3860..4315
                     /locus_tag="MB219_000125"
     CDS             3860..4315
                     /locus_tag="MB219_000125"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCC8425866.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            4312..5070
                     /locus_tag="MB219_000126"
     CDS             4312..5070
                     /locus_tag="MB219_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCC8425867.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            5571..5759
                     /locus_tag="MB219_000127"
                     /pseudo
     CDS             5571..5759
                     /locus_tag="MB219_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5805..6770
                     /locus_tag="MB219_000128"
     CDS             5805..6770
                     /locus_tag="MB219_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8425868.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            6792..7004
                     /locus_tag="MB219_000129"
     CDS             6792..7004
                     /locus_tag="MB219_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCC8425869.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            7079..7555
                     /locus_tag="MB219_000130"
     CDS             7079..7555
                     /locus_tag="MB219_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HCC8425870.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            7552..9360
                     /locus_tag="MB219_000131"
     CDS             7552..9360
                     /locus_tag="MB219_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCC8425871.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            9437..9652
                     /locus_tag="MB219_000132"
     CDS             9437..9652
                     /locus_tag="MB219_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HCC8425872.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            9744..10607
                     /locus_tag="MB219_000133"
     CDS             9744..10607
                     /locus_tag="MB219_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425873.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            10644..11180
                     /locus_tag="MB219_000134"
     CDS             10644..11180
                     /locus_tag="MB219_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCC8425874.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            11194..11616
                     /locus_tag="MB219_000135"
     CDS             11194..11616
                     /locus_tag="MB219_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCC8425875.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            11546..13945
                     /locus_tag="MB219_000136"
     CDS             11546..13945
                     /locus_tag="MB219_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCC8425876.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            13977..15968
                     /locus_tag="MB219_000137"
     CDS             13977..15968
                     /locus_tag="MB219_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCC8425877.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            15991..16242
                     /locus_tag="MB219_000138"
     CDS             15991..16242
                     /locus_tag="MB219_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCC8425878.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            16232..17461
                     /locus_tag="MB219_000139"
     CDS             16232..17461
                     /locus_tag="MB219_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCC8425879.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            17458..19539
                     /locus_tag="MB219_000140"
     CDS             17458..19539
                     /locus_tag="MB219_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCC8425880.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            19688..23608
                     /locus_tag="MB219_000141"
     CDS             19688..23608
                     /locus_tag="MB219_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCC8425881.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            23609..24553
                     /locus_tag="MB219_000142"
     CDS             23609..24553
                     /locus_tag="MB219_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425882.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRNQLSDSKQAASPKKAAARTKKNELEV"
     gene            24557..24760
                     /locus_tag="MB219_000143"
     CDS             24557..24760
                     /locus_tag="MB219_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCC8425883.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(25060..25296)
                     /locus_tag="MB219_000144"
     CDS             complement(25060..25296)
                     /locus_tag="MB219_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8425884.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(25479..25865)
                     /locus_tag="MB219_000145"
     CDS             complement(25479..25865)
                     /locus_tag="MB219_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCC8425885.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(25847..27175)
                     /locus_tag="MB219_000146"
     CDS             complement(25847..27175)
                     /locus_tag="MB219_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCC8425886.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(27136..27465)
                     /gene="mobC"
                     /locus_tag="MB219_000147"
     CDS             complement(27136..27465)
                     /gene="mobC"
                     /locus_tag="MB219_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCC8425887.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(27723..28094)
                     /locus_tag="MB219_000148"
     CDS             complement(27723..28094)
                     /locus_tag="MB219_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8425888.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            28239..28424
                     /locus_tag="MB219_000149"
     CDS             28239..28424
                     /locus_tag="MB219_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCC8425889.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            28582..28749
                     /locus_tag="MB219_000150"
     CDS             28582..28749
                     /locus_tag="MB219_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425890.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            29367..30026
                     /gene="vanR"
                     /locus_tag="MB219_000151"
     CDS             29367..30026
                     /gene="vanR"
                     /locus_tag="MB219_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCC8425891.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            30029..31372
                     /gene="vanS"
                     /locus_tag="MB219_000152"
     CDS             30029..31372
                     /gene="vanS"
                     /locus_tag="MB219_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCC8425892.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            31543..32349
                     /gene="vanY"
                     /locus_tag="MB219_000153"
     CDS             31543..32349
                     /gene="vanY"
                     /locus_tag="MB219_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCC8425893.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            32362..33171
                     /gene="vanW"
                     /locus_tag="MB219_000154"
                     /pseudo
     CDS             32362..33171
                     /gene="vanW"
                     /locus_tag="MB219_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         8783 a         8280 c         8776 g         7332 t
ORIGIN      
        1 ttttccataa aaatacatat tttaaatatg aaagtacaat tggaaataga ttaaaacgat
       61 aaaataaaat tgtcaaaacg tcagagagga tggtggagaa tgaatcaatg tgtaaaaaag
      121 ctgattgcgt cgattttgtt tgtcttccct ctcttgatgg tgacatccgt ttttgccgat
      181 gtagagaaaa caaacgaatc aacgcaatca gagaatcaaa gttttgacaa gactactttt
      241 tcagaagaat cgattacttc tgaaactaca gaatctcaaa gtgaggcttc tactaataaa
      301 aggcaagaag aaaataagac aagtgagacc tcagaagtag ctgaacaaac gtctgatagc
      361 agtgaagcgg cactggcaag agctgtttca gcggatgggt acagcgaagc tgccacaaca
      421 gaagaactgg cgcagcttct cgcagacgaa tcagtaacta agatccgact gattcagcct
      481 ttgactttag acagggaatt agagatcaaa cgagatatcg ttattgattt tggtggattc
      541 gctcacaatt tcggcacaca ccatatttat atcaatgaga tacctcagtc gatcgagttc
      601 cagaatttta aaggaacagc tgcacatcca ggtggattga caccaaatat cgatggaaat
      661 gccattatca ttgcttatat gaccgatttt tggggaagca gatatcattt tacgggagaa
      721 ataaaattta ccgggacact tgatttgtat gatgggtcca aattaggact gatttacgct
      781 cctcgtgcaa ctgtcacatt agatggtgtt tccggtgtgt tggatgtcca accagtgaag
      841 gaaggactga atgccgctcc gggtaaagcc tatttttgta gaagctatca attgaatgtg
      901 atcaatggta gtcaacttta tggaccgtat ctagggaaat tctacggttt tctagattca
      961 ggagatgaga ctggtagtgg aaaaacagcg ccaggaatcc atatcttaag cggttcaaag
     1021 gtctctcttg attatgatcg ggcagatggc gttagcgatg aaggagaggc agtcgatacg
     1081 ctacctggta atgtcatgtt tgaagtcagt ggttcgggaa gcgaattttc ggtaaataca
     1141 aagatcaata ttaccaatga caacaatcgc ggaatcatcc aaatgagagg ttcaggatcg
     1201 aaagtcactg tatcgaatgg tgggaaaatc accatcaata cagcaactac gggcggattt
     1261 cgcttgcaag gagattctag tcaaatccat gtatcaagtg gtggacaaat ctcggtcaaa
     1321 atggctggag atgacgatca acccgggaac aatgggatgc gttttgtagg aaaaggcctt
     1381 tctttgatcg tagaaggagc cggatcagtt atcgacatcg ataagcagag tgggcgttcc
     1441 agcgcagttc gttttgagaa tggtacgcaa aagctgacag taaccaatgg aggctcattg
     1501 agggtaagaa atacaggtga tggcaaatct tacgtgaaca gactcaatca aggaaatcaa
     1561 gcgatccaat tttatgacgc ttctggtttt ctagaaagtc caggttcagc tgattttaca
     1621 gtcactggag aaaaatcaaa tatcgatata cgcgcagatt ccggagcaac tgtagataca
     1681 actggagata ttgatttaaa ttttacggca ggtgaaaaaa cttatttagt catggtcggg
     1741 aaaacagggg aagataagac cggcatcatc agtggggatg tgttgaatgt ggacttagaa
     1801 tctcctacct attttgattt ccaaaacaaa cggattggaa gcgagaccaa tggcataggc
     1861 ggctgggtat tccaaggaaa tggagattct tctttaaaga tcgcccattc agaaatcgct
     1921 ttatggcgaa aaacaggcgc aacattcgat aactttgaaa aagacccgga ttatttttct
     1981 gatatgacag acttatcgat caatgggacc gacttaggca atttcgtatc aagccctagt
     2041 gcaggtttaa caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta taatcgaatc
     2101 agtgccaata atcagctccc gatgattgaa tccttaagag tgccaacgaa tgcagataaa
     2161 aaaatctatg ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag agatgcttgg
     2221 acaggcgaag tagaagtgac tttacgtatt acttacgcag atacgacgaa atcatctgta
     2281 gaaattactg ctctcacgca aggacaaaca gatgaaaatg aaggggcagg atataaccca
     2341 tggggagaag gcgaacaagg cggcttgttc gaagttcaag tacccaatga tgagagatta
     2401 aaaacggagg atcaagtgca agttgttgct gctaaaaaag taagaggtgg ccaagaagtg
     2461 acagaactgg actctgccaa aacgacagtc gatgtgattc ctcctgaacc tgctaattta
     2521 agtaccacat tgatcaatgc agcttctcgc acgatcacag gtacaggtga agcaggagcc
     2581 ttggtttcat tgaaaaaaaa tcaagctgtt ttgtcagaaa caactattga tgatacaggg
     2641 gaatttgttc tttctatccc agaaaatcaa ctttttgcag gagatgtatt agaaattgta
     2701 ttaaacgacc aagcaggaga agctactgct aagggtgtgc agaacaagcc aagtaccaac
     2761 gatgaaattg ggaatcacaa tccaagtgat acgccagttg tttatcatga tgccccgcct
     2821 tttgcacctg ctacgaagat cgtagtagaa ggtggtctct catttcttgc cccttccaaa
     2881 ctcgattttg gtcagatcaa agcaactggt ttgacacaag aagcatttgg ccaaatggat
     2941 atacaggaaa aattatggat ttatgatcag cgagaaacga aaaatccgtg gatcttaacg
     3001 ataaaattat ctcagccttt cacaaatgat gcaggatttt ctatgcagga tgtcctatat
     3061 ttcaagaaag gagaggagta tcaattagtg aatgaagaag aactggaaat tgtcagagga
     3121 caaaattcaa cgaatgtgca agcagattat tcagaatatt taaataacgg tcgggcgttt
     3181 aaacttgtct taaccaaaga aaatcagata gctggtaatt actcagcaga gataacatgg
     3241 acattagcag atgcgccgac agaataaaga tcaaggagga tgattgatga atcgaatatg
     3301 gaataaaagt catcgaaaca actgctcttt acttatgatc cttctccttg gattcatcct
     3361 ttggcagaca cctgtccatg cctccgaatt caattttgcg gtagattttg aattaccggc
     3421 taatcagatt gataagaaca aaacatatat aaatgtaagg atggagcctg ggaaaataca
     3481 ggaaatcccc tacacggtct ttaaccgaac agataaatca gtaacagttg agacattgat
     3541 ccactccgct acgacgaatt caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt
     3601 tgatgaaaca ttaagttatg atatgaaaaa cttagcaaaa acagtgggag aaataactgt
     3661 tcctcctcaa agtgcggtca ctgagacact ttcactgaaa gcgccagaaa aagagctggc
     3721 tggtgtggtc gcaggaggga tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag
     3781 tacagttggt gtcgtcaatc agttttctca agtgaaagca atcttgattt gaaatagcga
     3841 aaaagagtct tccccagatt tgaaattaaa aaaagcttat gcggatcaaa gcgatggaag
     3901 aaatgtgatt aaggcgaatc ttcaaaatcc aaatgcttct tatatgttcg gcttgactac
     3961 atcggttgaa atctacaaaa aagaaacaaa agagccttac ctttcaataa aaaaagaaaa
     4021 aatagatgta gcacccaata caaattttga tttacttgtc ccattagaag gaaaaaaatt
     4081 agctccggga cattatcaag cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct
     4141 ccataccgat tttgaaataa ctgatgatgc agcggaccaa ttgaatgaaa aggatattga
     4201 tttaaaaaat cagccagaaa catttgactg gcgatggctg atacttgttg gagtcattct
     4261 tattggctta tttgctggat tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa
     4321 acaattgcat taagtaccgt tctagctgga agtctattat tatgtacaca aagtgttttt
     4381 gctgagaata agagctcagc cactacagaa ggaacgattt ctgtgaccaa accagatgct
     4441 gcaaatcctg aagacgttgt tccagttcct cctgttgatc caaatgatcc tgatactcct
     4501 tttacacctg acgaaccaaa tcagggaaca tccggattac tgacgattga ccaagctcca
     4561 gatcttgatt ttggaacaat caagttaggt acagcaaaaa cagtatatgc tgcattacaa
     4621 agcggaacgg acagtactgg agcacaaaca caagtacaga atttcgtaca agtaacggat
     4681 aaaagcggaa attatgccgg ttggaaatta tcagtcaaac gaacagaatt tattggtaca
     4741 acatctgata cgaataaact aactggttca aaggtgaaat tcaaaaacgc cgtgattcga
     4801 aatagcgcaa ctaatagcag tggaatgcca tctaccgttt ctggaactgg cgctgaagga
     4861 atcgaaatcc cagcagatca ggcagtagat ctagtaactg caggagcaaa tgaagggatt
     4921 ggcacatgga tttattcatt aggagagaat gtggaacaag ggagacaatc agtggaatta
     4981 tttgttcctg tatccaatta tgctgtagac aactacgttt caacattcac atggtcgtta
     5041 acagatgctc ctacagaaga ggaagcgtaa aatcatatca acgatttcag ggagtggagc
     5101 cgattcaagg cccactccct gaaatctgct atgcttacgt ctcagctctt attcatcaac
     5161 tcgaagcagt tttttggaag tcatggaaac gttcctttgt cttttcaaag atctttcgac
     5221 caacgagtaa ggagtcaccg ttgaaaaatt ctaacacata atgagcagaa tcaattttct
     5281 gcaattgcga taagttgata cagtaagatt ttaaaagtaa aaattttagg ttatcgctga
     5341 aagccccgga aatacgggca tttcgcagga tatgagaact caatttacta ccaatttact
     5401 actttcggat tgagccgcat atatgctgaa ccatactgct aatacccgcc accccaaata
     5461 cggcacatca taaattgaat acgacaccgt aaacgcggcg ttcctctttc ccgtcatggg
     5521 agaacaggac gccgcttttt tatgcccaaa tagagaaagg aggcgacgcc atgctattta
     5581 cttccggccc tgaccgggcg tttgaacggc tgatgcagga aaagcccggt ttcgatcact
     5641 acgacggcgg cggggcggac gcgccggagg attgcggcac ctgccgattt taccgcccgg
     5701 attggaaata tcaattctgc cagtttgcag agtgtcccta tcagccgggc aagctgaccg
     5761 actatgcagc ggtcaaattc acagtgaaag gagcgaacaa cgctatggca gttttccgtg
     5821 tggaaaagaa caaaggctat acggtgatgt caaatcacca cctgcggaat aagaacctga
     5881 ccttaaaggc caagggcctg ctgtcgcaga tgttgtcctt gccggaggat tgggactata
     5941 ccctaaaagg actgtcctat atcaaccggg aaagcatcga cgcgatcagg acagcggtat
     6001 gggagcttga aaaagctgga tatatccggc gtgagcaggg ccgtgacgca aaaggcaaaa
     6061 tggccgatat ggtctatacc atctacgaac agccggtatt ggaaaacccg gtattggaaa
     6121 atccaacatc ggataacccg gtgttggaaa atccgacatc ggataatccg gcgtcggaaa
     6181 atccaacgca attaaataaa gaagtacaaa gaactgactt atcaaaaaaa gaaaaaataa
     6241 ttacagatgg acaaagtacc cattccattc ctatcccatc cccgacccct gcccctatgg
     6301 agggcgaggc agcggctacg ccgccggaac ggaaaaggac aggaaaggat gaggctgtac
     6361 ggatttatcg ggaaatcatt ttggagaaca tcgagtatgc ctatctcatt caggacagga
     6421 gcattgaccg tgaacagctt gacgaaatcg tagacctgat gctggaaacc gtctgcaccg
     6481 cccgaaagac aatccgtgtc gccggggacg actaccctgc cgagctggtg aaatccaagt
     6541 acatgaagct gaccggcgaa catatccgct ttgtccttga ctgtctgcgg gagaacacaa
     6601 cgaaagtccg caatatcaaa caatacctgc gggctatgct gttcaacgcc cccagcacca
     6661 tttccaacta ctacacggcc cttgtcgcgc acgacatggc acagcccgat tggggaaagc
     6721 ctaaatccgg cctgccggac tattcctgcg gcgagggcga aagcctgtaa cgagaaaagg
     6781 aggacgaccc tatgaagcag ggcgcattga tttttgacga gcgaacagac cgttacgaca
     6841 tccgctttga tctggccgac tattacggcg gcctgcattg cggggagtgt ttcgacgtga
     6901 tgatcggcgg caaatggagg cccacccgca ttgaaatggc cgaaaactgg tatcttgtcg
     6961 gcatccgggc cgacgacctt tccggcctgc gggtgcggat ttgacctata tctacctgcg
     7021 gcgtctggtg gttccgcctt agacgccgct tttgactttc tgacaaggag ggatgcgatt
     7081 gcaggaagaa gttactcaaa agaccattgc cctgtccatg aaaacgggca agctcacggc
     7141 tcaagtgctg caagcggcgc tgaaaaagta cctgcaacac cgggccaagg gcaaaaacac
     7201 gctgcaccac ggccagcaga gcctaaagca gcttaaaaag catggggctg atctctccaa
     7261 cattgagatc accgaggcca acattggagc gttcaagcca tgcgccaaga aatacggcgt
     7321 ggattttacc ttacgaaaag ataagaccac ccagccgccc cactacgttg tgattttcaa
     7381 ggccaaggat gcagataacc ttgatctggc gtttcgggag tttaccgcca agacgctttc
     7441 caaggagcag cggccctcta tccgcaaggt tttgtccgct gtgaagcaaa aggcggcggc
     7501 ccagaccaag cagcgggcca aggagaaaat caaggaaagg gggctggaac tgtgaagcct
     7561 gacgtaaaga aattgctgat cctgaacctc ccgtatctga tttttgtgta cctgtttgcg
     7621 aaatgcgggg aggcgtaccg gctggccgct ggtgctgacc tgtcggggaa gctcctgcat
     7681 tttgcggatg gttttgcggc ggcttttgcc aacccgctgc caagcctgca cctgtttgac
     7741 ctgtgtatcg gtatcgccgg tgccgtgatc gtgcggctgg tggtgtactg caagagcaag
     7801 aacgtgaaga aataccgcaa aggcatggag tatggcagcg cccgctgggg gacggccaaa
     7861 gacatagcgc cttacatcga ccccaagttt gaaaataata tcctgctcac ccaaacggaa
     7921 cggcttacca tgaccggcag gcccaaagac cccaagacag cgcggaataa aaacgtgctg
     7981 gtgatcggtg gcagcggttc agggaaaacg agatttttcg tgaaacccaa cctgatgcaa
     8041 tgctttccga ccacggacta tccgacttcg tttgtggtga cagatccgaa aggcactttg
     8101 gttctggaaa ccggcaggat gttccagcag gcgggctacc gcattaaaat cctgaatacc
     8161 attaactttt ccaagtccat gaagtacaac ccctttgtct acatccattc ggaaaaggac
     8221 gtgctaaagc tggtaaatac cctgatttcc aacaccaagg gcgaggggga aaagtcagcg
     8281 gaggattttt gggtgaagtc ggaacggttg ttttattcgg ccctgatcgg ctatatctgg
     8341 tacgaggccc cagcggagga aatgaacttc tcaacgctgc tggaaatgat aaacgccagt
     8401 gaagcccgcg aggacgaccc ggagtttcaa agccccgtcg atctcatgtt tgagcgtttg
     8461 gagcagaaag accccgacca ttttgcggtg cgccagtaca agaaattcct gctgtccgca
     8521 ggcaagaccc gttcttccat cctgatttca tgcggtgcca gacttgcgcc ctttgacatc
     8581 cgggaggtac gcgagctgat ggaggacgac gaaatggagc tggacaccat cggggacgaa
     8641 aagacggtgc tgtatctcat tatgagcgat acggacacga cctttaattt cattctggct
     8701 atgctccaaa gccagctaat caacctgctt tgtgaccggg ctgatgatgt gtacggcggg
     8761 cggctgcctg tccatgtacg cctgattttg gatgaatttg ccaacattgg ccaaataccc
     8821 aatttcgata aactcattgc caccattcga agccgtgaaa tatcggcctc tattattttg
     8881 cagagccagt cgcagttaaa agcgatttac aaggacgccg ccgagatcat ttcggataac
     8941 tgcgatagtg tgcttttcct tagtgggcgc ggcaaaaatg ccaaggagat ttccgatgcg
     9001 ctggggaaag aaacgattga cagtttcaac accagcgaga accggggaag ccaaacgtcc
     9061 cacggattga actatcaaaa attaggaaag gagttgatgt cccaagacga aattgccgtg
     9121 atggacggcg gcaagtgtat cttgcagcta cggggtgtgc gcccgttttt atcggagaaa
     9181 ttcgatatta ccaagcatcc ccggtacaaa taccttgccg acgccgacaa gaagaatacc
     9241 tttgacgtgg acagattttt gactatcacg cgccggaaac ggcagcaagt ggtaacgcag
     9301 gatgaagtat tcgacctgta tgaaatcgac ctgtcggacg aggacgccgc cgctgaataa
     9361 acagcggctt ttttcattcc aacaacaaaa tcaaaaaatc attatgagcc ggaaaccggc
     9421 agaaagaggt aaatttatgg cattttttaa ttccgcagtt actactttgc agactatcgt
     9481 gatcggactg ggcggcgctc tgtgcgtatg gggcggcatc aatctgttgg agggatacgg
     9541 gcaggacaat ccgggggcaa aatcgcaggg tgtcaagcag cttgtcgctg gcggcggcgt
     9601 ggccctgatc ggtgtcactc tggttcccct gctgtccggg ctgctgggct gatcctggcc
     9661 tttgtccctg tccaaaccct taactaatcg cctccgcgtc ctcccggcca gaccgggagg
     9721 gcgcgggaaa ggaggtgcta cccgtgatta gtgaaatcat tgaagaatgg ataaaaggca
     9781 tcctcatgga cggtatcacg ggcaacctgt ccgggctgtt cgatacggta aatgccaaag
     9841 tcggagaaat cgccgccgac gtgggacgaa ccccgcaggc atggaacagc ggtattttca
     9901 atatgctgca cagcctgtcg gaaaccgtga tcgtccccat agccggggcc atactcgccc
     9961 ttgtcatgtg ctatgagctt atccagatga taacggaaaa gaacaatatg cacgactttg
    10021 acagctccat gttcttccgc tggatattca aatccgcgtt tgcaatcctg atcgtaacga
    10081 acacatggaa catcgtcatg ggcgtattcg acgccaccca aagcgtcgtc aatcaaagcg
    10141 ccggtgtgat tatcgggcag acatccattg attttaacac cctgatacca gaccttgaaa
    10201 gccaattgga ggctatgagc gtgggcgctc ttttggggct gtggttccaa acccttgttg
    10261 tgggcctcac catgaatatc ctttccatct gcattttcct tgtgacctat ggccgcatgg
    10321 tggaaatcta tgttgtcacg tcgctggggc ctatccccct tgccacaatg ggcaacgcgg
    10381 aatggcgcgg catgggccaa gggtatctga aatccctgct ggcgctgggc ttccaagcct
    10441 ttttaatcat ggttgtggtg ggtatctacg cggtgctgat acagaatatc ggttccgctt
    10501 ccgacatatc cggcgcaatc tggggctgta tgggctatac ggtgctgctg tgcttctgcc
    10561 tgttcaagac cggcagtata tcaaaggccg tattcggggc gcattgatcc caaaatccaa
    10621 gcatacacag gaggaacttg tttatttcaa cgtacaacat catttacgct gacccgccgt
    10681 ggcggtatga ctgcaaaaac gtacagggcg cagcggaaaa tcactattcc accatgagca
    10741 ttgacgagct gtgcgccctg ccggtggaac ggctggcgtc caaagactgc ctgctgtttt
    10801 tgtgggccac attccccatg ctgccggaag ctctgcggtt aatcaaagcg tggggctttt
    10861 cattcaagac cgtggctttt gtatggctga aacagaaccg taaaagcctt acatggtttt
    10921 acgggctggg ccgctggaca aggggaaacg cggaaatctg cctgctggca acacacggca
    10981 agccgaagcg tcgttctgcc tctgtccatc agtttattat cagcccgatt gaacaacaca
    11041 gcaaaaagcc ggatgtgacc cgtgaaaaaa tcgtagagct ggcgggcgat ttgccccgcg
    11101 tggagctgtt tgccagacag aaaacccccg gctgggacgt gtgggggaac gaggtggaat
    11161 gtgaccttat cctgacgtga aaggaggtgc caaatggctt atgtaaccgt ccccaaggac
    11221 ttaacccatg tgaaaagcaa ggttgtgttt gggcttacca aacggcaact gatctgtttt
    11281 tccggggcgc tggtgatcgg cgtcccctta tattttctca cccgtgacta tcttaccaac
    11341 agcgcggcgg ccctgctcat ggtatttgcc atgctgccgg gctttttgct tgccatgtac
    11401 gagcgtcacg gccagcccct tgaagtggtg attatgcaga tcatccagtg ctgcttcatc
    11461 cagccaaagg agcgtcccta tcagaccgac aacacctaca ccgcccttgt gcggcaatac
    11521 cagatggaac aggaggtaaa ggccattgtc caaaaaggaa atcccaagag aaaccgaaaa
    11581 aacaaagctg acccgcgccc agcgaaaaga aattgacgcg gtaatccgta aatataaggg
    11641 cgacggcagg ccccacacgg cgcagcagag catcccctac gaggtcatgt acccggacgg
    11701 cgtgtgccgg gtatcgcccg gcgtcttttc caagtgcatt gagtttgcgg acatcagcta
    11761 tcagcttgcc cagccggaca cgcagacggc tatctttgaa aaactgtgcg acctgtataa
    11821 ctatgtggac gcctccattc atatccagtt ttctttcctc aatcggaaag ttgacccggt
    11881 gcagtacgcc aagagctttg agatcgcgcc gcagggggac gacttcgacg acatccgggc
    11941 cgagtacacc ggcattctgc aaaagcagct tgcaaacggc aacaacggca tggtaaagac
    12001 caaatacctg acattcacca tcgaggccga aagcgtgaaa gcggcgcgag ccagattgaa
    12061 gcgcatcggc tttgacctgt taggctattt caagagcatg ggggccgtag cccatgtgat
    12121 ggacggctgg gaacggctga acctgctgca tggcgtgtac cacccggacg gcgagatatt
    12181 caattttgac tggaagtggc tggccccgtc cggcctctcc accaaggatt ttattgcccc
    12241 gtcctccctg tgcttcggca atgcaaagac attcggcatg ggcggcaaat acggcgcggt
    12301 aagtttcctg caaatcctgt ccccggagct gtcggacgat atgctggccg acttcctcaa
    12361 tacggaaagc ggtgttttag tcaatctcca tgtgcaggcc attgaacaga ccaaggcgat
    12421 caagaccatc aagcgcaaaa ttaccgacct tgacgctatg aagattgcgg agcagaaaaa
    12481 ggccgtccgc agcggttacg atatggacat tcttcccagc gaccttgcca cctacggcga
    12541 ggacgccaag aagctgctga caaagttgca gacccgcaat gaacggctgt tccagttgac
    12601 gttccttgtc ctcaacgtgg ccgacaccaa gcaaaagctg aataacgacg tgttccaagc
    12661 cgcaggcgtg gcccaaaagc ataactgtcc ccttgtccgg ctggactacc agcaggaaca
    12721 gggccttgca agcagcctgc cgctgggcgt caatcagatc aagatacagc gcagccttac
    12781 gacttccagc gtggccgtgt tcgtcccctt tgtgacgcag gagctttttc agggcggcgc
    12841 ggccatgtat tacggcatca acgccaagtc ccgcaacatg attatgctgg acaggaagca
    12901 ggcccgttgc cccaatgcct tgaagctcgg cacccccggc agcggcaaat ccatgagctg
    12961 taaatccgag atcgtcagtg tgttcttaac gacgcctgac gatattttta tttcagaccc
    13021 ggaggccgaa tattaccccc ttgtcaaacg gctccacgga caggtaatcc ggctgtcgcc
    13081 taccagcaag gactttgtga acccgctgga catcaacctg aattattcgg aggacgataa
    13141 cccgctggcc ctgaaatccg actttgtgct gtcgttctgt gagcttgtca tgggcggcaa
    13201 gaacgggctg gaagccattg aaaagaccgt gatagaccgg gctgtgcgcg tgatctaccg
    13261 gccctatctg gcagaccccc gcccggagaa tatgccgatc ctgtccgacc tccacaaagc
    13321 gttgttagat cagcatgtcc cggaggctga ccgggtggcg caggcccttg acctgtatgt
    13381 gtccggctcc ctgaacgtat ttaaccaccg taccaacgtg gacatcggca accggcttgt
    13441 gtcctttgac attaaggagc tgggcaagca gctcaaaaag ctggggatgc tcattgtcca
    13501 agaccagata tgggggcgcg tcacggccaa ccgaagccaa ggaaaggcca catggtattt
    13561 tgccgacgag ttccatttgc ttttgaaaga ggaacagacg gcggcctatt ctgcggagat
    13621 ttggaagcgg tttaggaaat ggggcggcat tccaacaggt gccacccaga acgtcaaaga
    13681 cctgctttcc tccccggaaa ttgaaaatat ccttgaaaac agcgacttca ttacgctgct
    13741 caatcaggcg tccggggata gaaaaatcct tgcggaacgc ttgaatttgt ccaccgaaca
    13801 gcagaagtac atcgacaatt ccgaacccgg cgagggcctt ttgatctttg aaaacgtggt
    13861 gctgcccttt accaacccca tcccgcacaa cacccagctt tacaagatta tgacgacccg
    13921 cttaaacgag gtggcgggag tatgacccgg cgacgccaca aaggaggtga accaccatga
    13981 aagagctgaa agccaaggcg aaagtgacgc aggccatgac ccgcgacggg ctgacgctgg
    14041 aaaatcaggc caccggcgag gtggagaaca tttccagccg ggaggccgaa caggatttgt
    14101 ccgcgccggg gccgggcggc accgccgata agctgctgga acgggccgac gccatccatg
    14161 acggccataa ggccaagaaa gccgcgaagc aggccggggc tgtggccgcc gagggggacg
    14221 ctgccctgca ccggccatcc gcccggctcc aactgtcgga ggaagaacgg gccgacccgg
    14281 atttacagcc ctatatccga aaatcggaca aacgggccga taagctggat aaggcccgcg
    14341 ccgccatccc caagaagcgc gtcccggtca aggaaaccgt ctttgacgag gcaagcggca
    14401 aagcaaagac cgtcctgcac tttgacaaac aggacaaggg gcctccctcc ctgaaaccca
    14461 atccggcagg ccgtcctgtt tccgaggttt ttctattcgg ccacgggaaa atccatgaag
    14521 tcgagcatga aaacgtgggt gtggagggcg gccataaggg ggaggaactt gccgagcggc
    14581 aggccggaaa agccatccgc agcaccatcc ggcaccacag gctgaaaccc tactgggacg
    14641 cggaaaaggc cgagcgcagg ctattgtctg ccaacgcgga gtatttctac cagaaatctt
    14701 tgcgggataa cccggagatc gcacaggcgg ccagcaaccc catttcccgg ttctggcaga
    14761 aacagcagat caagcgccag tacgccaagg ccgcccggac agcgggccag accgcaggcg
    14821 cggcggcaca gggcgcggcc aagacagccg gggcctccac gaaagcggcc aaggccgcag
    14881 ccaaagaaag tgaacgggcg gcctcctttg tggcccgcca ctggaaaggg gcgctggccg
    14941 tccttgtgtt ggggcttttg atgctgctta tcatcggcgg cttacagtcc tgcaccgcca
    15001 tgttcggcag cgccggaagc ggcatcacgg cgtcctctta cttttccgag gatagcgata
    15061 tgttggccgc cgaggacgcc tacgcggagc tggaagccga gctgcaagac tatctggaca
    15121 actacgaggc cacccatgac tatgacgaat accattttga ccttgacgag atcagccatg
    15181 acccctatgt gctgatttcc attctgtcgg cgctccatga cgggatattt accatcgggg
    15241 aggtacaggg tgatttgtcc atgctctttg acaagcaata tatcctgaca gaaaacgtcg
    15301 tggtagaaac ccgttaccgc accgaaaccg atacatggac ggacgcggag ggcaacaccc
    15361 atacagacac ctaccaagtg ccgtatgact attacatctg ctacgtcacg ctggaaaact
    15421 tcgacctgtc ccatgtgcca gtctatatca tggacgagga aacgctaagt ttctattccc
    15481 tgtatatgtc cacgctagga aaccggcctg atctgttttc cggcagccag taccccaacg
    15541 cctccaccct gacgccgccg acctactacg acgtaccccc ggaggctttg gaggatgcca
    15601 cctttgccgc catgctcaaa gaggcggaaa aatatatcgg ttatccctat gtgtggggcg
    15661 gcagctctcc cagcaccagc tttgattgca gcggcttcat ttcgtgggta atcaatcata
    15721 gtggatggaa cgtgggacgg ctgggggcgc agggcctata taacatctgc acccctgttt
    15781 ccagttcgca ggccaagccc ggcgaccttg tatttttcaa gggaacctac gacacccccg
    15841 gcgtgtccca cgtcggcctg tatgtgggca acaatgtgat gctgcattgc gggaacccga
    15901 tttcctacac gaaccttact tcaaactact ggcaatccca tttttacagt tacgggcgtt
    15961 taccttaacg ccagaaagga gctttcaatt atggcaacca aacttgaccg catcgaaaag
    16021 gacatccaga aaaccaagtc taagatcgcg gagtttcaga aacagcttcg ggagctggaa
    16081 acgcagaaaa ccgaacagga gaatttgcag atcatccagc ttgtgcgcgg catgaacatg
    16141 aagccggagg aatttgccgc gttcttacgc agcggcgcaa tgcaggccgc ccctgccgca
    16201 accccttacc atgaacagga ggacaatgcc catgaagaat aagaccagac ggatttcccg
    16261 tattctcccc gcacttctgg ccgtgatgct ctgcgtcacg gcctttcccg ttaccgccct
    16321 tgcaggcggc aatgacccgg caccggcccc gctgccggag gctacaaccg aacccgctac
    16381 gggcggtttg gagcctgaaa cagacgaaac cggcaccgcc acggacggga cagaaaccga
    16441 ggccgaaaaa gataccggcc ctctgaccgg caaggacatc accgacctgt tttccgcgct
    16501 gttcggttcc aaggtgagca ttgccgccac ggacgacggc atccagatca ccaccggcac
    16561 cgacaaaaaa gaacctgaac agaccggcac cgtcaccacc aacggcggca ggctgaatgt
    16621 ccgcaccggc gcggggcttg acaagaccgc gttcacccag cttcccaatg ggacgacggt
    16681 tgaggtagtc ggcacagacg gcgactggat taaaattctc ttgccggagc gcatcggcta
    16741 tgtccattcc gactatatga ccgtcagcga aaaggaagta gcggctaccg gcgagggcgg
    16801 tttttccctc tccattgacc cggaggaaat tgcttctctg ctggaactgt tcaatggcgg
    16861 cggggccagc gccgccctgt ccccggacgg gaacctgtca ctgattgacg acatcggcag
    16921 cgccgcgaaa aatggcaagc agtttatcac cgtggaaacc aaaaacggca atgtgtttta
    16981 tctgattatt gaccgggacg acgagggaaa agaaactgtc catttcttaa atcaggtgga
    17041 tgaagccgat ctgctgacgc tgatggagga cggggaaacc gtcacccccg ctgctgtctg
    17101 ctcctgtacc acgaaatgca aggccggggc tgtgaacacg aattgcccgg tatgtaaaac
    17161 caacctgacc gagtgcagcg gcccggagcc gcaggaaccc cagccggagg aacccgaagc
    17221 cccggaggaa gaacccaagg gcggcgcggg cggcctgatc gtgttcctgc tggtggcgct
    17281 ggcgggcggc ggtgcggccc tttactattt caagttcaaa aagcccaagg ctgaaaccac
    17341 cggcagcgac gatttgggcg agtatgattt tggggaggac gaggatttgg aggacgagga
    17401 acccgaaacc gagtttgacc cggaagctga ttttgtgagt gaacaggagg acgaggaatg
    17461 acatataaac ttgtaatcgc ggaaaaaccc agcgtggccc gcagcattgc gggcgtgatc
    17521 ggggccacgg agaaacacga cggctatttg gaggggaacg gctatctggt gagctggtgc
    17581 atcggacatc tggtgtcctt tgccgacgcc ggacgctatg acgagcgttt caaaaaatgg
    17641 cgctatgagg atttgcccat tctcccggaa acatggcagt acatcgtccc cgacgacaag
    17701 aaacagcagt ttgacgttct ctgttcgctg atgcagcgcc ccgacgtgac cggccttgtg
    17761 tgcgccaccg acgccgggcg cgagggggaa ttgattttcc gtttcgtcta ccagatggcg
    17821 ggatgccaaa agccctttga gcgcctttgg atttcctcta tggaggacag tgctattaag
    17881 gacggctttg cccacctgaa accgggcggc gactatgatc cgctgtatca atcggccctg
    17941 tgccgggcca aggccgactg gctgatcggc attaacgcca cccggctttt ttccgtcctg
    18001 taccacaaga acctgactgt gggccgcgtc cagacgccga ccttaaaaat gctggtggac
    18061 agggacgcca agattaccga tttcaagaaa gagaaatatc acatcgtcca tatcacggcg
    18121 ggcggcgcgg acgcggtaag cagccgcttt tccgacgctg ccgaggcaaa caccgtcaag
    18181 gcggcttgtg caaaagcgca ggccgtttgc gtttccgtga cgcgggagaa aaagacggaa
    18241 cagccgccca agctctatga ccttaccacc ttgcagcggg aggctaaccg gcttttcggc
    18301 tataccgcca agcagaccct tgactatgcc caggcactgt atctaaagaa gctgctgacc
    18361 tatccgagaa cggatagtca atatcttacc gacgatatgc tgcccgccgt ggaaagcctt
    18421 gtgtccggcc tgtgggagct ggtgccgttt gccaagggcc tgaacctttc cccgcagttt
    18481 gaccgcatcc tgaacagcaa aaaggtgtcc gaccaccacg ccattatccc caccgctgaa
    18541 tttgtgaagc agggctttgg cactctggcc gagagtgaaa aaaagctgct gtccctgatc
    18601 tgctgcaagc tcttatgtgc cgtggccggg ccgcatatct atgaggccgt caccgccaca
    18661 tttacctgtg ccgggcagga gtttaccgcc aagggcaaga ccgtcctttc ccccggctgg
    18721 aaagagattg aacgccggtt ccgttcttcc ttaaagaccg acgccgacga ggacgccgag
    18781 gcggtgcggg agctgccgga gctttgcgag ggccagacct ttgcagacgt ggccgcctct
    18841 gtcaccgagc atttcaccac gccgcccaag ccctacaccg aagatacgct tttgtccgct
    18901 atggaacggg ccggggccga ggatatgccg gaggatgccg agcgcaaggg attaggcaca
    18961 ccggccaccc gcgccgccat tctggaaaag ctggtgcaga tgggctttgt ccagagaaaa
    19021 ggcaagcagc tcattcccac caaggacggc atcaatctgg ctgtggtgct gccggaggcg
    19081 cttacttctc cccagcttac tgccgaatgg gaaaaccggc tgaccgagat cgccaagggg
    19141 caggccgatc ctgacgaatt tatggacggc atcgagacgc aggcgcggga gctggtgcag
    19201 acctattcct gtatcagtga ggaaaagcag aaattgtttc aggccgagcg tgttcctatc
    19261 gggacgtgtc cccgctgtgg tgaggctgtc tatgagggca agaaaaacta ctactgcgga
    19321 aaccgggcct gccagttcgt catgtggaag aatgaccgat tttttgagga acgcaagaaa
    19381 gcgttcaccc cgaagatcgc cgctgcgctg ctcaaaaccg gcaaagtcaa ggtaaagggc
    19441 ctctattccg taaagaccgg caaaacctac gacgggacgg tgcttttggc tgataccggg
    19501 ggcaagtacg tcaactaccg cattgaacac aggagctaaa ggacagtaac acagcaagca
    19561 tagggagtgt ggctacactc ccggtaaaat cccccggccg cgtccctgcc gggccgggga
    19621 tttttgcttg ttggggaagt ccccaacccc ctattttttg aaacagaaca gagaaaggag
    19681 tgaccgcgtg aacgaagtat tttctatcca agtccataac cggcagtttt tggaacaggg
    19741 caaacaaggc gtctggctgg atttgcccac cacaaccgaa acgctgcaag ccgccctgcg
    19801 ggatgttggc attactgccg acaatccgca ggattttttt atcaacgggt attcctgccc
    19861 ggaggacaga caccttgcca ttccctatga catggtgctg gccgccgacg tggacgagct
    19921 gaatttcctt gccgcccggc tgggccagct tgacgccgcc gagatcgcag agctgaacgc
    19981 cgccctgcaa aatccccgtg gcggctttga gaatatcggc cagatcatcg actacaccga
    20041 aaatgtggat tactatgtgc atcttcccga cgttaggggg gatggacagt tgggcgacta
    20101 ctacctgaac cgttccggca tggtggatat gccggaggaa tggaaagccg ggattgatac
    20161 ggcgcagttt ggacgccaca ttgcccagca ggaacaaggc gtttttaccc agtacggcta
    20221 tctggtgaga agcggcgacg aatggcagcg cgtccatgag ggccagccgg tgccggagga
    20281 ataccgggtg atgcactacc cccagccgga agctgaccgg gaggaaccgc aggcccagca
    20341 gccggaggca tccgaaagcc ccgacaaagc gccgcccccg gttatcccga tcatgctgga
    20401 cggcatgaac agcaaagaac gcatgaaagt gattactgac cgtctggaaa ccggcgtaca
    20461 ggagcttttt gaaagtagcc gctatatggc ttacttatcc gctatgtcca agatgcacga
    20521 ttacagtctg aacaatacgc tgctgatcgc catgcagggc ggttcgattg tggcgggcta
    20581 taacaggtgg caggatgaat ttgaccgcca tgtgaagcgg ggcgagaaag gcattaagat
    20641 actggccccg gccccgttca aggttaagca gcagatggag aaaatcgacc cggccaccca
    20701 gcagcccgtg atcggcgcgg acggcaagcc cgtgaccgaa gaacaggaag tgacggttcc
    20761 ggcctttaag gtggtgacag tctttgacat cagccagacc gagggcaagg attttcccat
    20821 gccctatatc agtgaactta ccggcgacgt gcagcagtat caggatttct ttgccgcgct
    20881 ggaacggaca tcaccctatc ccataagcat tgagccgatt tcagacgggg ccaagggccg
    20941 ctgcttctac gcaaaacagc gcattgccat caacgagggc atgagcgaat tgcagaccct
    21001 aaagaccgcc atccatgaga ttgcccatgc caagctccac gccattgacc gggaggcccc
    21061 cgccgaggaa caggcggccc ggccagaccg cagcacccgc gaggtacagg ccgaaagcgt
    21121 ggcctatacc gtctgccagt attacgggct ggacacgtcg gactattctt ttggctatgt
    21181 ggccgattgg agcagcggca gggagttatc cgagctgaaa gcctcccttg aaaccatccg
    21241 ggccacggcc agcgaattga taaccgagat cgacggccat tttgcccagc ttcagcagga
    21301 gcgcgaggcc cggcaggaac aggaggccgt cacggttgac gttaccccgg aggcagcggc
    21361 cccggcgcag gccgaggaag aacaggcccc ggcccttgat ccggcagccg agccgcttgt
    21421 gactgtgcta tggagcgaaa gcccccagct aaaggacggc cagcaaatgc cgttgcatga
    21481 ggccgacgcg ctgtttgccc gtctggatgc agcatatccg gcagaggcag gctatgacaa
    21541 aaccgcgttc cgcattgact gtatctttca gggcgagccg gacagctacg aggggcggca
    21601 ggattttgga gatagggacg gttccctgat cgaacatatc cagagctacc accaatttta
    21661 cgcgcaggac gaaaactgga aaaagcatgt aatcgctaca accggcatag agggctggga
    21721 gcaggacaag gcccatcggg atatgctgtt aggcgagttt gtcccctata tgaagctgca
    21781 ttgcagcctt gcccaaatgg aacagacggc gcagggccgc ttacagtccg gggaaaccct
    21841 gacgccggag gaaacggcct atttgggggc gcttctctct tatgtgcagg agtgccgccc
    21901 catgctcaat cagggccagt atcagttgcc ggagccgccg aaactggctg acttcgacca
    21961 gagcttgcag gactacaaag cacaggtaca ggccgagatc gcgcaggagg ccgccgcagc
    22021 cggtatgacc gtggaggaat acgcggcggc tggctatgaa gcaccccaac aggacacctt
    22081 ttccatctac cagcttcgag gcggggagga aacccgcgac tatcggtttg agccatacga
    22141 gcgtttacag gccgcagggc tggccgtgga cagtgccaac tatgaccttg tttatacggc
    22201 accgctggat aaaagcacgt ctttggagga tatttaccgc acattcaaca tcgaccaccc
    22261 ggcagatttt aggggacaca gcctttccgt ttccgacgtg gtggtgctgc accagaatgg
    22321 acaggacacc gcccactatg tagacagctt cggctatatg gaagtgccgg agtttttgcg
    22381 ggaaaatccc ctgcgtaccg ccgaactgtc tacggagcag aacgaaaaca tgattgacgg
    22441 cattatcaac aatacgccgc ccgaaccgca gccggaggaa cccgaacagc tatcttttga
    22501 ggatgtggca gttgcttccg gccagacaca ggaggaaccc ccggaggctg cgcccccgca
    22561 aacggctgtc cgctattatc ccatcaatga gggagccgcc cgccgcgcca aggaagccat
    22621 gagcttttct gactaccggc ccggcagcgc cacggcggaa taccggcact atgtcgatca
    22681 ggccgtggaa attgcggagc gccagaaaaa gcgtgtggag ccagagtatc acgaaaagat
    22741 agacagcctc cttgacacct acgcccgcaa gctggccgag aacatgaata agggctatga
    22801 gattaccgcc cgcgtcccct ctgtcctgat tgccgggccg tccaatttcc cggtacgggc
    22861 gaaagaaaag cagaacgccg ccagtgaccg caacatggag gaatttcagt acattcaggg
    22921 cttgcttgac aaaatccgca gcaccggcat gggtggtatc agcgccgacg atccgcaggc
    22981 cgtttccaag ctggaaaaga aactggaaaa gctggaagcg tcgcaggagt tgatgaaggc
    23041 cgtcaatgcc tactaccgca agcatgggac gctggacgga tgcccccatc tgaccgaaag
    23101 aggcattgaa aacctgaaag cggatatggc gtcgggctgg cactatgaga aaaaaccgtt
    23161 ccagtcctgg cagctctcca acaataacgc cgagatacgc cggttaaagg gccgcattga
    23221 ggaattgacc cgccaaaagg aggcggccta tgtgggatgg gagtttgacg gcggcactgt
    23281 ggagatcaac cgggtggcca accgcctgca aatcttcttt gaggggaagc cggatgctgc
    23341 tgtacgcgac gagctgaaaa gcaacggttt ccgctggtcg cccaaggccg gggcatggca
    23401 gcggcagtta aacgacacca caatccgcgt cgctgaccgt atcaagtgca tccagcccct
    23461 ttccggcgaa aagcccagcg ccttacagct tgcggcccgc agggagaaag aaaagccctc
    23521 tatccgggcg cagctcaacg cggccaagac cgaacaggcc aagcagccac cggcgcggga
    23581 aaaatcaaag ggattggagg tagagtaaat gcagaagttt gaaaatgtgg acgttctggc
    23641 cgcgctggaa cagatcatgc ggcagaacac cgcgttttat caaagtgact ttggcattga
    23701 caaagacatt atcaggcggg atgccgccag cgatcaggcc gcagacaaga ccctgctctg
    23761 gatgtcccgg ccctccggga cgtactgttt ccgggaacgt gatgttttcc tgaaagacac
    23821 ccgacagtac aacacatgga aattttacgg ggagcagacc cgtgacaaga tactggccta
    23881 cgcggtggag ctgaccggca cacagggcgg cactatccgg ggcaatctct atgagctgga
    23941 ctatccgcag catttccggc acgtcattga aaccgcccag ccggtgagtg tgaaccggct
    24001 cttttatgag catggcaccc gtgacatccc ggagagccag cgttttgacg gaagccctga
    24061 ccgggtattg ggaaactttt tgtactatga ggcccagccc catgacccgg ctgtgctgca
    24121 ggaggcgcta cggacggagc agcacagccg ggggcgggcc gtccccggcg atttcaaagt
    24181 ccacgtcacc gctttgcatg acagccggat tgaaaccgag gcccggcgta ttgtggacag
    24241 cctgaaagat ctgtccgcgc ctaacagccc caataaaacg catttcatgg tggagatctc
    24301 cccgcacttt gcggcgattg cttccaccaa ggataatgac cggctctttg ccatgctgcc
    24361 ctataaatcc ctgtgtttta ccgggatgaa aggcaggcat ggcgtgtatg ccgtgattaa
    24421 ccgggacgag cgccgggatg tgagtattcg caggccccgg ccctctatcc gcaatcagct
    24481 atccgacagc aaacaggccg ccagcccaaa aaaggcggcg gccagaacca aaaagaatga
    24541 attggaggta taaggaatga tgcactttac cgtggaggaa gaaaacctga tctgtatgta
    24601 ccacaatgca gaccggcgca gaacgataaa caggctcacc gccgtcatgc cggttatgga
    24661 tggggatatg cgggcgcagg cccagcagac cctttccaag ctggaacgca tgacggatgc
    24721 cgactatgac ggccagaagt tccacttcac agacgaataa cgaacagcag acgccgggcg
    24781 cggcggccat gtggtcgctt gcgtccggcg tcttttcttt ttgcctattc ctacgcattt
    24841 agaacccggt attgtcgggg ataggatgaa gtattcgctt gcccctgcaa acatgcggga
    24901 gaagccttgc agcgggcggt tttcaatccc ttaaatgcgt aggttgcagc tataattaga
    24961 cccctgaaac agatatagcc gcgcagcggg tgtatctgtt tcaggggtgt gcaggtgata
    25021 gccgcaaagc ggcgtaagag ggtgcagccc tcttgccgtc tatctctgtt ccagcgggaa
    25081 gctcggcaat gcttccagca gtttcaggga aatgcgctgc cgtatatctt cgtcaatgac
    25141 tttcttcttt ttgccgtttg cctgtctgat ctccaccgtg gaaagctcgt tgatgtagtc
    25201 agcataatgg gccaacactt tttccgtggc ccacctctca ccggcaacgg cggctttgat
    25261 cgtttcatag tccagcagct tttcttgtcc gtccatcctg atacccccgt cagactgatt
    25321 ttttctatga taccatattc ggaagcatgg aaccagagaa aacggcgctt ttctgctggt
    25381 aaattctacg catttagaac agcacttcgg cggggatagg ataaaacacc cacatacccc
    25441 gctgaccatg ccggaaaagc cttgaaatat aagggctttt acagctctaa gtgcgtaggt
    25501 ttccagtatc ttttccgctg gcgctcggcc tctttgcggc ggcgcatccg tttggagcag
    25561 gccgcgcagt atttggcccg gttagacttc ggcgtgaacg tggccccgca ctcacagcag
    25621 cgtttcactt cgtcccggtc tttggtgatc tcggcataca gggccgcatc cagcgggagg
    25681 acagcggcct taaaccagcg gcacagaagc gaataggaaa tgctctgtac gcaaatacat
    25741 tcttcgccgt cgtccagcaa caggcagttg ccgtcacaat agttgcagca ctcatgcgtc
    25801 agcttccggg ccttgcgata ttgagggtaa ttcattcgtg gaatactcat agctcttgcg
    25861 tccattcctt tcccggcgtc atacgcaaaa tgccgtccac attctgcttg atgatgccgt
    25921 attcctgcat ctgctttttc gcttcgccgt acttctcata cagccgttcc ttttcctcgg
    25981 ccaatcgctt gtactcggct ttcagggcgt acagatcggg gagctttttc acaccctgtc
    26041 cctttagggc ctttgccgca gcttcataca aaatcaacgt gctttcatgc tcacgccgga
    26101 attttgcctt atccgctgca ttgcggtatt ccatagccac gggccagagc tgcttgtagg
    26161 tagaaatatt ctttatcagc accgccatat ccgcaagccg gtgttccata gctttgaggg
    26221 tagcggcggc ctcgtcgctg gcggcggtga tctcggccac tttgctttcc aaatccgggt
    26281 aggtgtctat cccatgttca gtcaggaaat tcaaagtctt tgcggcctgc ttcaaattgt
    26341 ggagcttcgc ccacctctca tagcctgccg actgctgggc cttaatgttg ttttccagat
    26401 caatccgcaa agaaatcccc ttgcgctctt tgggcgctct ggtgataacg cggcctttga
    26461 tacgctcttt gatggcttcc tcggtgtagg cttcccccaa tgtcttgcag cgggtaaagc
    26521 gttcctgtcc gggagcgcgg aaagaaataa actttcccgg cttgatctca tagccctgcg
    26581 cctgcatgag ccgcaggaaa tcatcaaaat cttttgcctg cgggatagcg gcgtcaatgg
    26641 ctgctttgag cttcgctttc cagctcgtcc ctttccgctg ggcgtcccac tcggcatagc
    26701 tcttgccctt actctggccg ggcttcacca cggacaatcc gttttccttg cacagcctgt
    26761 cgctggtgcg ccggatatag gcatagctct gtttgttgga aatgtacttg cgctgattta
    26821 ccatatccac ggcacagaaa ataatgtgat tgtggacgtg gcccttatca atatgggtag
    26881 tcagcacata gggatatttc ccttgcagca cttcctcggc aagctgcttc ccgatctcat
    26941 gggcctgttc cggcgtggct tcgccggggg caaaggattg tatcaggtgg tggcccagat
    27001 tattgccctt ttggtatgcc tgcgccagca gcatttcaaa ttcaatgtcc gctgtttcat
    27061 aagagcaggc aaaggaggcc accagcagct tgccgtcagt tttgtccggg ttctcgatgt
    27121 agtcaagggc ttttttcagc gttgacttga taggatgtat ctttgtaact gccatatctg
    27181 cgccagcgcc ccctttatat cctcgatgtc ctgtgcgtac accggcccca taccgttcag
    27241 cctctttgca atctgattga cgttgacgcc gatcttctgt atctcggcgg tctgcgcccg
    27301 gatgtcgctg gtgtccatgt tgacgatata gccgtcaatg agcatcttcc gggcgtaggc
    27361 tgaaaagttc ttcgtatgca gcagcgccat tttctgttca atcatagccc gttcctccgg
    27421 cgtcacaggg acgcgcagca cgatttttcg ctttctgttt gccatgcttc accgtccttt
    27481 ccattcaggg gtttggggat ttccccaaca agcaaaacgg acgcggcccg gcagggacgg
    27541 ggcaggccgt ttttccggga gtggctacac tccctatgct tgctacgtta ctgtttctcc
    27601 actaacaaga cgtttccaaa gccccaaact acccgcaaaa tagccctctg gaaagaaaaa
    27661 agtgcaaaaa aatagaagct acgctgtgca gctcccgcta aaagtaaaac agaggatgcc
    27721 agttacacat cctccgtttc attcttcttc ggcttttcaa ttagtatggc gctgtcaatc
    27781 gcgccctcga tgatcggcag gtattcttcg gggcagagtt tcagcttgtg gctgaccctt
    27841 tgccgcagtt cgctttcctc ccgcatgatc tccgggttaa agtagcgttc cacaggaagc
    27901 ccgcaggctt taataagctg tatgacaacc ggcagactgg gaatggcccc gtcgttttcg
    27961 atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt gcgcgaaacg
    28021 cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc gggacaatct
    28081 tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc tgcccgtgga
    28141 atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct tttattcttc
    28201 gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg acgttgaaag
    28261 aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt gctgcgggac
    28321 gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact gcggaaaagc
    28381 cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat gaaaccggcg
    28441 cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg aaaaactata
    28501 agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg aaacagaaag
    28561 aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga aatggaaaat
    28621 gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag aattgaaaag
    28681 gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc tggtctaatg
    28741 cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt ttgaggaaaa
    28801 tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg acacggatga
    28861 tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt attaggactg
    28921 aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg ccctgcctgt
    28981 gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt tgctgaggct
    29041 tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt ggatcagaat
    29101 atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt ttgacaaccg
    29161 ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct tgatttaatt
    29221 cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc ccaatgacgg
    29281 atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag actgttatgt
    29341 tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc gaggatgatg
    29401 atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag gtggatgcct
    29461 gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa ctggttattc
    29521 ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt cgtgcgaaaa
    29581 atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa atccgggcgt
    29641 ttgatctaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt ttactaaagc
    29701 gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt gtcggcaggc
    29761 tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg cccctgacac
    29821 gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc ttaacgcatg
    29881 aaatcatttt gtcacgtata tggggatatg actttgagag tgatggcagc acagtccaca
    29941 ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc attcgcggtg
    30001 taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca ttaaggtttt
    30061 ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac tgtttgcaca
    30121 gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat cctatcagcc
    30181 attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg cagggctgtt
    30241 tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa gcgtactcta
    30301 tgccacacca aatgccaata catcaaatag ttttagaccc gactttcttt atgtggtaca
    30361 tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat tgctttatca
    30421 agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca gccttttatg
    30481 cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg acagtgcgaa
    30541 taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg agcttggcgc
    30601 actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg caaggctgga
    30661 ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct ttgcggcagc
    30721 ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg gaatgcttga
    30781 aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca aaatgatgga
    30841 taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg atgggagaat
    30901 cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc tgcccgattt
    30961 tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag ccgggcaaat
    31021 tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat tgaatgcagt
    31081 tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg ctgaaaaatg
    31141 ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc caaggctgtt
    31201 caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa acggtttagg
    31261 acttgccatc gtacaaaaaa cgctggatgc catgagcctc cagtatgcgc tggaaaacac
    31321 ctcggatggc gttttgttct ggctggattt accgctcaca tcaacattgt aaatatttaa
    31381 aacttaaatg attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga
    31441 aaactacaga ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca
    31501 gccacaaatc gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat
    31561 cattccaatg cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt
    31621 ctatgtgcgt tcattatttc gttcacagtc tgcacgctgt ttttggggcg gagactggct
    31681 tccgtattgg aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact
    31741 gacgtagtgg agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa
    31801 caggaatgga gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg
    31861 gagcttgagc aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa
    31921 gatttgtttg atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga
    31981 acaacagaaa aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc
    32041 tacacctctg cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc
    32101 gagcatcagc ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac
    32161 gaggtttata gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca
    32221 ccagacaaga cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc
    32281 atcgaagctg ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca
    32341 gaaaaatgag aaaaggatgt tatggacaga aaaagattga cacagcgttt cccgttcctg
    32401 cttccaatga gacgggcgca gagaaaaatg tgcttttatg cgggaatgag atttgacggc
    32461 tgtcgctatg cgcagacgat aggggaaaaa tcgctttccc atttgctctt tgaaacggat
    32521 tgcgcattat acaaccacaa taccggattt gacatgatat accaggaaaa caaggtgttc
    32581 aacttaaagc tggcagcaaa gaccttaaac ggcttattga taagaccggg ggaaaccttt
    32641 tctttctggt ggctggtacg ccatgcggac aaagataccc cctataaaga cggccttacg
    32701 gtgaccaatg gtaaactcac caccatgtcg ggcggcggta tgtgccagat gagcaattta
    32761 ctattttgga tgttcctgca tacgccattg acaattatcc agcgcagagg tcacgaagta
    32821 aaggagtttc cagagccaaa cagtgacgag attaaagggg tggatgcaac catttcagag
    32881 ggctggattg atttaaaagt gcgaaacgat accgactgca cctaccaaat atgagtgacc
    32941 ttagatgatg agaaaatcat cggtcaggtg tccgccgaca aagagcccca agcattatac
    33001 aaaattacaa atggcagtat ccagtatgtc cgtgaaagtg gcgggattta tgaatatgcc
    33061 caggttaaac ggatgcaagt tgccttaggt accggggaaa taatagattg caagctgctt
    33121 tatacaaaca aatgcaaaat ctgttatccc ctcccggaaa gtgtggatat t
//
LOCUS       DAHEYX010000005        32217 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHEYX010000005 DAHEYX010000000
VERSION     DAHEYX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.005"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            25..393
                     /locus_tag="MB219_000155"
     CDS             25..393
                     /locus_tag="MB219_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCC8425894.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            563..994
                     /gene="mraZ"
                     /locus_tag="MB219_000156"
     CDS             563..994
                     /gene="mraZ"
                     /locus_tag="MB219_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCC8425895.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1015..1974
                     /gene="rsmH"
                     /locus_tag="MB219_000157"
     CDS             1015..1974
                     /gene="rsmH"
                     /locus_tag="MB219_000157"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCC8425896.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1979..2380
                     /gene="ftsL"
                     /locus_tag="MB219_000158"
     CDS             1979..2380
                     /gene="ftsL"
                     /locus_tag="MB219_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCC8425897.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2381..4573
                     /locus_tag="MB219_000159"
     CDS             2381..4573
                     /locus_tag="MB219_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCC8425898.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4599..5561
                     /locus_tag="MB219_000160"
     CDS             4599..5561
                     /locus_tag="MB219_000160"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCC8425899.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5575..6936
                     /locus_tag="MB219_000161"
     CDS             5575..6936
                     /locus_tag="MB219_000161"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCC8425900.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6962..8050
                     /gene="murG"
                     /locus_tag="MB219_000162"
     CDS             6962..8050
                     /gene="murG"
                     /locus_tag="MB219_000162"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCC8425901.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8065..9285
                     /locus_tag="MB219_000163"
     CDS             8065..9285
                     /locus_tag="MB219_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCC8425902.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9441..10766
                     /gene="ftsA"
                     /locus_tag="MB219_000164"
     CDS             9441..10766
                     /gene="ftsA"
                     /locus_tag="MB219_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCC8425903.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10789..12030
                     /gene="ftsZ"
                     /locus_tag="MB219_000165"
     CDS             10789..12030
                     /gene="ftsZ"
                     /locus_tag="MB219_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCC8425904.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12047..12724
                     /locus_tag="MB219_000166"
     CDS             12047..12724
                     /locus_tag="MB219_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCC8425905.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12738..13337
                     /locus_tag="MB219_000167"
     CDS             12738..13337
                     /locus_tag="MB219_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCC8425906.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13352..13639
                     /locus_tag="MB219_000168"
     CDS             13352..13639
                     /locus_tag="MB219_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCC8425907.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13688..14470
                     /locus_tag="MB219_000169"
     CDS             13688..14470
                     /locus_tag="MB219_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCC8425908.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14491..15198
                     /locus_tag="MB219_000170"
     CDS             14491..15198
                     /locus_tag="MB219_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCC8425909.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15460..18258
                     /gene="ileS"
                     /locus_tag="MB219_000171"
     CDS             15460..18258
                     /gene="ileS"
                     /locus_tag="MB219_000171"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCC8425910.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18307..19833)
                     /locus_tag="MB219_000172"
     CDS             complement(18307..19833)
                     /locus_tag="MB219_000172"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCC8425911.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19848..20495)
                     /locus_tag="MB219_000173"
     CDS             complement(19848..20495)
                     /locus_tag="MB219_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCC8425912.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20679..21008)
                     /locus_tag="MB219_000174"
     CDS             complement(20679..21008)
                     /locus_tag="MB219_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCC8425913.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21185..21913
                     /gene="sfsA"
                     /locus_tag="MB219_000175"
     CDS             21185..21913
                     /gene="sfsA"
                     /locus_tag="MB219_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCC8425914.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21929..22942
                     /locus_tag="MB219_000176"
     CDS             21929..22942
                     /locus_tag="MB219_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8425915.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22942..24219
                     /locus_tag="MB219_000177"
     CDS             22942..24219
                     /locus_tag="MB219_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCC8425916.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24282..26984
                     /locus_tag="MB219_000178"
     CDS             24282..26984
                     /locus_tag="MB219_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCC8425917.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27136..27453
                     /locus_tag="MB219_000179"
     CDS             27136..27453
                     /locus_tag="MB219_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8425918.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27483..27803
                     /locus_tag="MB219_000180"
     CDS             27483..27803
                     /locus_tag="MB219_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCC8425919.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27911..29371
                     /locus_tag="MB219_000181"
     CDS             27911..29371
                     /locus_tag="MB219_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8425920.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29439..29660
                     /locus_tag="MB219_000182"
     CDS             29439..29660
                     /locus_tag="MB219_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425921.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29691..29873
                     /locus_tag="MB219_000183"
     CDS             29691..29873
                     /locus_tag="MB219_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCC8425922.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29873..30286
                     /locus_tag="MB219_000184"
     CDS             29873..30286
                     /locus_tag="MB219_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCC8425923.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30409..31590
                     /locus_tag="MB219_000185"
     CDS             30409..31590
                     /locus_tag="MB219_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8425924.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAELGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31661..31825)
                     /locus_tag="MB219_000186"
     CDS             complement(31661..31825)
                     /locus_tag="MB219_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425925.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31979..32052
                     /locus_tag="MB219_000187"
     tRNA            31979..32052
                     /locus_tag="MB219_000187"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32013..32015,aa:Arg,seq:cct)
     gene            complement(32121..>32217)
                     /locus_tag="MB219_000188"
     CDS             complement(32121..>32217)
                     /locus_tag="MB219_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCC8425926.1"
                     /translation="MSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
BASE COUNT        10783 a         5530 c         6852 g         9052 t
ORIGIN      
        1 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
       61 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
      121 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
      181 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
      241 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
      301 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
      361 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
      421 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
      481 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
      541 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
      601 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
      661 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
      721 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
      781 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
      841 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
      901 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
      961 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
     1021 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
     1081 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
     1141 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
     1201 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
     1261 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
     1321 tatgatctag gggtttcctc tccccagcta gatgaagcag aacgtggatt tagttatcat
     1381 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
     1441 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
     1501 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
     1561 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
     1621 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
     1681 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
     1741 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
     1801 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
     1861 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
     1921 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
     1981 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
     2041 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
     2101 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
     2161 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
     2221 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
     2281 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
     2341 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
     2401 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
     2461 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
     2521 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
     2581 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
     2641 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
     2701 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
     2761 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
     2821 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
     2881 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
     2941 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
     3001 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
     3061 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
     3121 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
     3181 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
     3241 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
     3301 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
     3361 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
     3421 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
     3481 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
     3541 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
     3601 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
     3661 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
     3721 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
     3781 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
     3841 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
     3901 tgaatatatg agagatgttg tcgaaagcga aaactacggt attgcttatg gtgtatatag
     3961 tgtacctgga tacaatgtat cggcaaaaac cggaacagcc caaatcgctt ctgatacagg
     4021 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
     4081 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
     4141 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
     4201 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
     4261 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
     4321 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
     4381 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
     4441 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
     4501 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
     4561 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
     4621 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
     4681 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
     4741 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
     4801 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
     4861 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
     4921 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
     4981 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
     5041 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
     5101 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
     5161 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
     5221 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
     5281 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
     5341 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
     5401 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
     5461 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
     5521 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
     5581 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
     5641 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
     5701 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
     5761 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
     5821 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
     5881 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
     5941 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
     6001 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
     6061 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
     6121 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
     6181 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
     6241 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
     6301 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
     6361 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
     6421 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
     6481 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
     6541 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
     6601 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
     6661 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
     6721 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
     6781 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
     6841 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
     6901 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
     6961 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
     7021 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
     7081 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
     7141 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
     7201 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
     7261 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
     7321 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
     7381 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
     7441 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
     7501 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
     7561 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
     7621 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
     7681 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
     7741 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
     7801 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
     7861 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
     7921 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
     7981 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
     8041 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
     8101 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
     8161 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
     8221 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
     8281 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
     8341 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
     8401 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
     8461 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
     8521 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
     8581 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
     8641 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
     8701 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
     8761 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
     8821 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
     8881 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
     8941 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
     9001 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
     9061 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
     9121 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
     9181 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
     9241 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
     9301 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
     9361 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
     9421 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
     9481 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
     9541 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
     9601 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
     9661 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
     9721 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
     9781 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
     9841 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
     9901 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
     9961 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    10021 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    10081 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    10141 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    10201 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    10261 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    10321 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    10381 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    10441 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    10501 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    10561 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    10621 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    10681 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    10741 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    10801 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    10861 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    10921 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    10981 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    11041 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    11101 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    11161 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    11221 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    11281 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    11341 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    11401 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    11461 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    11521 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    11581 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    11641 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    11701 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    11761 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    11821 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    11881 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    11941 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    12001 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    12061 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    12121 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    12181 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    12241 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    12301 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    12361 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    12421 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    12481 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    12541 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    12601 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    12661 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    12721 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    12781 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    12841 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    12901 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    12961 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    13021 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    13081 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    13141 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    13201 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    13261 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    13321 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    13381 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    13441 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    13501 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    13561 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    13621 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    13681 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    13741 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    13801 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    13861 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    13921 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    13981 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    14041 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    14101 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    14161 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    14221 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    14281 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    14341 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    14401 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    14461 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    14521 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    14581 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    14641 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    14701 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    14761 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    14821 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    14881 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    14941 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    15001 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    15061 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    15121 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    15181 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    15241 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    15301 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    15361 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    15421 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    15481 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    15541 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    15601 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    15661 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    15721 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    15781 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    15841 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    15901 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    15961 ggtaaaatgg ctgaaaaaag ctatatttac aaaggattga aaccaattta ctggtctccg
    16021 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    16081 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    16141 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    16201 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    16261 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    16321 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    16381 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    16441 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    16501 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    16561 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    16621 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    16681 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    16741 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    16801 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    16861 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    16921 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    16981 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    17041 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    17101 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    17161 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    17221 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    17281 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    17341 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    17401 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    17461 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    17521 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    17581 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    17641 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    17701 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    17761 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    17821 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    17881 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    17941 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    18001 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    18061 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    18121 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    18181 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    18241 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    18301 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    18361 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    18421 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    18481 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    18541 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    18601 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    18661 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    18721 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    18781 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    18841 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    18901 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    18961 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    19021 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    19081 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    19141 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    19201 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    19261 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    19321 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    19381 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    19441 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    19501 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    19561 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    19621 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    19681 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    19741 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    19801 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    19861 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    19921 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    19981 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    20041 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    20101 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    20161 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    20221 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    20281 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    20341 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    20401 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    20461 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    20521 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    20581 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    20641 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    20701 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    20761 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    20821 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    20881 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    20941 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    21001 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    21061 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    21121 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    21181 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    21241 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    21301 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    21361 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    21421 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    21481 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    21541 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    21601 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    21661 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    21721 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    21781 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    21841 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    21901 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    21961 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    22021 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    22081 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    22141 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    22201 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    22261 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    22321 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    22381 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    22441 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    22501 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    22561 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    22621 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    22681 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    22741 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    22801 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    22861 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    22921 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    22981 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    23041 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    23101 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    23161 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    23221 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    23281 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    23341 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    23401 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    23461 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    23521 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    23581 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    23641 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    23701 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    23761 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    23821 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    23881 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    23941 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    24001 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    24061 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    24121 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    24181 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    24241 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    24301 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    24361 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    24421 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    24481 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    24541 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    24601 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    24661 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    24721 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    24781 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    24841 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    24901 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    24961 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    25021 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    25081 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    25141 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    25201 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    25261 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    25321 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    25381 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    25441 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    25501 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    25561 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    25621 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    25681 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    25741 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    25801 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    25861 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    25921 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    25981 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    26041 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    26101 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    26161 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    26221 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    26281 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    26341 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    26401 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    26461 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    26521 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    26581 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    26641 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    26701 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    26761 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    26821 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    26881 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    26941 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    27001 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    27061 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    27121 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    27181 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    27241 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    27301 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    27361 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    27421 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    27481 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    27541 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    27601 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    27661 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    27721 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    27781 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    27841 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    27901 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    27961 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcacactgcc
    28021 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    28081 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    28141 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    28201 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    28261 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    28321 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    28381 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    28441 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    28501 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    28561 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    28621 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    28681 atatattgca gaaccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac
    28741 agtatttgtt caagtattct ggttctttgg tattcatgga ccaaatgtat tggcaccagt
    28801 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    28861 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    28921 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    28981 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    29041 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    29101 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    29161 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    29221 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    29281 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    29341 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    29401 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    29461 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    29521 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    29581 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    29641 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    29701 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    29761 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    29821 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    29881 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    29941 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    30001 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    30061 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    30121 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    30181 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    30241 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    30301 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    30361 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    30421 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    30481 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    30541 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    30601 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    30661 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    30721 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    30781 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    30841 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    30901 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    30961 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    31021 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    31081 gcggagttgg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    31141 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    31201 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    31261 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    31321 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    31381 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    31441 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    31501 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    31561 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    31621 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    31681 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    31741 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    31801 ctttttcttt tggcttatca ttcattggaa atgacctccc tacattattt cattctacct
    31861 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    31921 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    31981 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    32041 cagtcaggaa cgtacaaata acccctgtat cctttgcgat acaggggttt gttttgtgtt
    32101 tgtgccaaaa atgtgccaaa ctagttatca tttttatata aactatcgaa cacagcattg
    32161 attttttctt tatcctgatc ttcaagctct cgaacaatat gaagataagt agacatt
//
LOCUS       DAHEYX010000006        30085 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHEYX010000006 DAHEYX010000000
VERSION     DAHEYX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.006"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            260..598
                     /locus_tag="MB219_000189"
     CDS             260..598
                     /locus_tag="MB219_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425927.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(803..1042)
                     /locus_tag="MB219_000190"
     CDS             complement(803..1042)
                     /locus_tag="MB219_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCC8425928.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1018..1365)
                     /locus_tag="MB219_000191"
     CDS             complement(1018..1365)
                     /locus_tag="MB219_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCC8425929.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2097..2678)
                     /locus_tag="MB219_000192"
     CDS             complement(2097..2678)
                     /locus_tag="MB219_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8425930.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2861..3487)
                     /locus_tag="MB219_000193"
     CDS             complement(2861..3487)
                     /locus_tag="MB219_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCC8425931.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3509..4840)
                     /locus_tag="MB219_000194"
     CDS             complement(3509..4840)
                     /locus_tag="MB219_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCC8425932.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4995..5396)
                     /locus_tag="MB219_000195"
     CDS             complement(4995..5396)
                     /locus_tag="MB219_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCC8425933.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5482..5688)
                     /locus_tag="MB219_000196"
     CDS             complement(5482..5688)
                     /locus_tag="MB219_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425934.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5783..7285)
                     /locus_tag="MB219_000197"
     CDS             complement(5783..7285)
                     /locus_tag="MB219_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCC8425935.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7303..7758)
                     /gene="argR"
                     /locus_tag="MB219_000198"
     CDS             complement(7303..7758)
                     /gene="argR"
                     /locus_tag="MB219_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCC8425936.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7856..8473)
                     /locus_tag="MB219_000199"
     CDS             complement(7856..8473)
                     /locus_tag="MB219_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCC8425937.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8694..10067
                     /locus_tag="MB219_000200"
     CDS             8694..10067
                     /locus_tag="MB219_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8425938.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10142..11254
                     /locus_tag="MB219_000201"
     CDS             10142..11254
                     /locus_tag="MB219_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCC8425939.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11326..12963)
                     /locus_tag="MB219_000202"
     CDS             complement(11326..12963)
                     /locus_tag="MB219_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCC8425940.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13131..13739)
                     /locus_tag="MB219_000203"
     CDS             complement(13131..13739)
                     /locus_tag="MB219_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCC8425941.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14123..15274)
                     /gene="queG"
                     /locus_tag="MB219_000204"
     CDS             complement(14123..15274)
                     /gene="queG"
                     /locus_tag="MB219_000204"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCC8425942.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15293..16045)
                     /locus_tag="MB219_000205"
     CDS             complement(15293..16045)
                     /locus_tag="MB219_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425943.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16186..17136)
                     /locus_tag="MB219_000206"
     CDS             complement(16186..17136)
                     /locus_tag="MB219_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCC8425944.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17137..17976)
                     /locus_tag="MB219_000207"
     CDS             complement(17137..17976)
                     /locus_tag="MB219_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCC8425945.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17973..18719)
                     /locus_tag="MB219_000208"
     CDS             complement(17973..18719)
                     /locus_tag="MB219_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCC8425946.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18957..20327
                     /locus_tag="MB219_000209"
     CDS             18957..20327
                     /locus_tag="MB219_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8425947.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20382..21278)
                     /locus_tag="MB219_000210"
     CDS             complement(20382..21278)
                     /locus_tag="MB219_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCC8425948.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21392..21562)
                     /locus_tag="MB219_000211"
     CDS             complement(21392..21562)
                     /locus_tag="MB219_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425949.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21559..22236)
                     /locus_tag="MB219_000212"
     CDS             complement(21559..22236)
                     /locus_tag="MB219_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCC8425950.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22327..23232)
                     /locus_tag="MB219_000213"
     CDS             complement(22327..23232)
                     /locus_tag="MB219_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCC8425951.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23407..24831
                     /locus_tag="MB219_000214"
     CDS             23407..24831
                     /locus_tag="MB219_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8425952.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            24877..25908
                     /locus_tag="MB219_000215"
     CDS             24877..25908
                     /locus_tag="MB219_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCC8425953.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25915..27102
                     /locus_tag="MB219_000216"
     CDS             25915..27102
                     /locus_tag="MB219_000216"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCC8425954.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27258..28640
                     /locus_tag="MB219_000217"
     CDS             27258..28640
                     /locus_tag="MB219_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCC8425955.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28612..28998
                     /locus_tag="MB219_000218"
     CDS             28612..28998
                     /locus_tag="MB219_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425956.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29198..29866)
                     /locus_tag="MB219_000219"
     CDS             complement(29198..29866)
                     /locus_tag="MB219_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCC8425957.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8443 a         6153 c         5487 g        10002 t
ORIGIN      
        1 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
       61 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
      121 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
      181 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
      241 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
      301 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
      361 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
      421 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
      481 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
      541 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
      601 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
      661 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat
      721 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
      781 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
      841 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
      901 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
      961 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
     1021 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
     1081 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
     1141 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
     1201 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
     1261 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
     1321 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
     1381 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
     1441 tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc
     1501 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt
     1561 tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag
     1621 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat
     1681 ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa
     1741 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt
     1801 tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt
     1861 tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
     1921 cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt
     1981 tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt
     2041 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
     2101 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
     2161 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
     2221 ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
     2281 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
     2341 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
     2401 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
     2461 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
     2521 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
     2581 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
     2641 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
     2701 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
     2761 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
     2821 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
     2881 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
     2941 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
     3001 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
     3061 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
     3121 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
     3181 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
     3241 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
     3301 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
     3361 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
     3421 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
     3481 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
     3541 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
     3601 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
     3661 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
     3721 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
     3781 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
     3841 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
     3901 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
     3961 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
     4021 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
     4081 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
     4141 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
     4201 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
     4261 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
     4321 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
     4381 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
     4441 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
     4501 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
     4561 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
     4621 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
     4681 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
     4741 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
     4801 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
     4861 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
     4921 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
     4981 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
     5041 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
     5101 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
     5161 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
     5221 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
     5281 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
     5341 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
     5401 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
     5461 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
     5521 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
     5581 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
     5641 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
     5701 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
     5761 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
     5821 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
     5881 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
     5941 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
     6001 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
     6061 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
     6121 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
     6181 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
     6241 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
     6301 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
     6361 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
     6421 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
     6481 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
     6541 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
     6601 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
     6661 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
     6721 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
     6781 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
     6841 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
     6901 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
     6961 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
     7021 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
     7081 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
     7141 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
     7201 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
     7261 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
     7321 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
     7381 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
     7441 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
     7501 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
     7561 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
     7621 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
     7681 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
     7741 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
     7801 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
     7861 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
     7921 cgctgatgta aatctctttg tgttctttgg aagctctttc atagccttct tttgcgatga
     7981 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
     8041 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
     8101 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
     8161 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
     8221 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
     8281 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
     8341 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
     8401 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
     8461 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
     8521 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
     8581 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
     8641 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
     8701 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
     8761 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
     8821 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
     8881 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
     8941 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
     9001 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
     9061 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
     9121 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
     9181 catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg attatcggct
     9241 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
     9301 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
     9361 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
     9421 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
     9481 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
     9541 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
     9601 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
     9661 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
     9721 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
     9781 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
     9841 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
     9901 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
     9961 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
    10021 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
    10081 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
    10141 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
    10201 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
    10261 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
    10321 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
    10381 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
    10441 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
    10501 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
    10561 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
    10621 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
    10681 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
    10741 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
    10801 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
    10861 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
    10921 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
    10981 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
    11041 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
    11101 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
    11161 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
    11221 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
    11281 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
    11341 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
    11401 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
    11461 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
    11521 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
    11581 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
    11641 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
    11701 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
    11761 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
    11821 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
    11881 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
    11941 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
    12001 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    12061 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    12121 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    12181 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    12241 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    12301 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    12361 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    12421 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    12481 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    12541 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    12601 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    12661 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    12721 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    12781 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    12841 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    12901 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    12961 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    13021 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    13081 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    13141 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    13201 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    13261 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    13321 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    13381 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    13441 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    13501 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    13561 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    13621 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    13681 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    13741 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga
    13801 aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat
    13861 ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc
    13921 aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat
    13981 tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta
    14041 aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact
    14101 ttgaataatg ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt
    14161 ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa
    14221 gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga
    14281 tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta
    14341 gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag
    14401 ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt
    14461 ttggatatat ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt
    14521 gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt
    14581 tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg
    14641 cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac
    14701 agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct
    14761 caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa
    14821 atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg
    14881 gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga
    14941 tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag
    15001 caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa
    15061 gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat
    15121 cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt
    15181 cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt
    15241 tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc
    15301 tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg
    15361 caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg
    15421 aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac
    15481 ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat
    15541 acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc
    15601 caggcctagc aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa
    15661 taaattgact tttataatag gccgactgga tctttttgat tactccttct gctttgatga
    15721 ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc
    15781 caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta
    15841 aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg
    15901 tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt
    15961 gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat
    16021 atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg
    16081 aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct
    16141 tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt
    16201 ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc
    16261 tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc
    16321 cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac
    16381 cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg
    16441 actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata
    16501 cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact
    16561 ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg catttttttt
    16621 atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt cctccacata
    16681 ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat gttcttcacc
    16741 gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt ttccttcttc
    16801 ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa gattcaaacc
    16861 gttgtataaa ataacatctg catcgcttgc ctttttgatg tcttctggta gtacttcata
    16921 ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat ctgttccgac
    16981 ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact tttctttact
    17041 tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca agaaactaac
    17101 tgctaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt tctgaaataa
    17161 aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac atgctaacac
    17221 gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga caactgcact
    17281 tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg ttagcaagta
    17341 agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta ctgtctgtaa
    17401 actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt
    17461 cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa
    17521 gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca aatcgctttc
    17581 cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac tctgggcata
    17641 tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc
    17701 atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta gcatcccaaa
    17761 agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta cacctggaag
    17821 aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa taaaagaacc
    17881 gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg
    17941 ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc ttttactaaa
    18001 tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac ttcttgtact
    18061 ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata ttcttctact
    18121 ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag
    18181 atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc atctaaaaag
    18241 atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag ctgtccaccc
    18301 gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt caaagcttgt
    18361 cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt
    18421 cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc acttcgctgt
    18481 tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa ggtgacttta
    18541 ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt tcctgcaccg
    18601 tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat agttttcaaa
    18661 acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt acgttccaaa
    18721 tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta actaaaatgg
    18781 aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg cattttatga
    18841 aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat gaaaaacatg
    18901 tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg
    18961 aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc gcactaggtg
    19021 gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg acagggccct
    19081 cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg cgtgctttag
    19141 gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg agcgagtaca
    19201 tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat attgtcgtag
    19261 gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct gatttgccgg
    19321 caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca
    19381 aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg atcattttga
    19441 tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt caggcagtag
    19501 ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa ggatttttct
    19561 ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga atgactgctg
    19621 gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact atcggtcgaa
    19681 tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg tggaatcaat
    19741 tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt acatttgctg
    19801 caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat tcagggattt
    19861 tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct aaaaaatttc
    19921 tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca ggtggaatct
    19981 taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca gaaaatattt
    20041 tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta gtgattttga
    20101 tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat ccttttaaaa
    20161 tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc gtattgattt
    20221 tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt ttcttgcttt
    20281 acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag
    20341 cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa ttttagcgtc
    20401 tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa gatcgtttct
    20461 actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa aactacggtt
    20521 gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt tgtgtcttgt
    20581 gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga atcagaagat
    20641 gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg caaataaata
    20701 tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat caaagtatct
    20761 aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt tctgatccct
    20821 tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga taaaccagct
    20881 attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc aggcgtcttt
    20941 tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg caaaccattt
    21001 gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc acttgtttgt
    21061 ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa
    21121 ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa cacggcaact
    21181 gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc
    21241 atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac caatttttat
    21301 gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg tcgctgtgca
    21361 catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc caagattgtc
    21421 cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc agtttcttta
    21481 tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca tctaattgtt
    21541 ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa
    21601 gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt gtttttcaga
    21661 ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt caatccgctg
    21721 agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta gcttctccca
    21781 acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa gataggtcat
    21841 atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg tatattcttc
    21901 ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga ttcctagttc
    21961 tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa tttttaaatg
    22021 agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat gctgggccaa
    22081 gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt ccaaatgatg
    22141 ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta aagattcttc
    22201 gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt tttagtcttc
    22261 gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc attttaacca
    22321 gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt tcaaggtatc
    22381 caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg tagccgtttc
    22441 tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc ctacttgata
    22501 ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc gattgatccg
    22561 taccccgatc aaatattcct tgatactggt gcccatatga tttttgaata ctcgcgacaa
    22621 atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt tttcagcata
    22681 atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct tttgtacacc
    22741 tccgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg ttaatgctgt
    22801 taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta aaataatgtc
    22861 gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga aaagcgactg
    22921 ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca tccacggatc
    22981 ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca gatcaccttt
    23041 tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca tcacgacatg
    23101 caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg ttttagagaa
    23161 accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt ttaaaaaaat
    23221 cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa aagttaaata
    23281 aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag tcattaaaag
    23341 atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag gagaggaata
    23401 cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc ttttggacac
    23461 gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag ccagatgttc
    23521 gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag cttagttgta
    23581 ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat cgataatact
    23641 cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt tagttccgta
    23701 ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac gactttattt
    23761 atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt taaggatatc
    23821 gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga aaaatttgct
    23881 acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt cgttgtccct
    23941 atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc ttcaggctgg
    24001 ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat cactgccttg
    24061 ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat
    24121 gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct
    24181 tatgcgattg ccaatgcagc aacaactgcc gtattattct acttcttcaa atatgtactt
    24241 ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg tttagtagct
    24301 gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta ccttggaggg
    24361 atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag tttgccaatc
    24421 gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt atccgctttg
    24481 atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg taacgaagct
    24541 gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc taacgggatc
    24601 gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc tgatatcact
    24661 gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc tttgatgatt
    24721 ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca tgcacaaatc
    24781 gttgagaaat tggaaaacaa attggctcca gctgatacaa tggaagatta acttcacgat
    24841 gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga aaaagatttt
    24901 ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact ttctatctct
    24961 gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt tttgggattt
    25021 gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc
    25081 gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca attagccaca
    25141 gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg
    25201 tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac tacaagtccc
    25261 gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac tttgacaaaa
    25321 gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt attcaatccg
    25381 acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga tcaacatgag
    25441 ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc tataggggta
    25501 aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt
    25561 tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc atttttctta
    25621 aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa aagaatccaa
    25681 gtagatattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt cggcacagaa
    25741 acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt tgagactcaa
    25801 acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa agccggatca
    25861 gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact catcatggaa
    25921 atcaaaagaa gcttaaacac aaaatttgcc gaaatattcg gacctaaaaa aactacacaa
    25981 tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa tggaggccat
    26041 gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg agacgataat
    26101 aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt tactttagat
    26161 gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat ggtccttaaa
    26221 ttaaaagaag ctggacactc aatcgatcat gggtttgagt tattagtgga aggaacaatc
    26281 cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg tgttgtatta
    26341 gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg taaaaaagta
    26401 gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat cggcttcggt
    26461 gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat ggttccagtg
    26521 aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga attggcagat
    26581 tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact
    26641 ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac tattgaccaa
    26701 atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa tcagcggact
    26761 ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca actgttaaat
    26821 gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt agatacacta
    26881 gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg agctggattt
    26941 ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc
    27001 tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc acacattgga
    27061 aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa tcaacgatct
    27121 cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg agccttttgc
    27181 tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt tctaattgtg
    27241 ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat gaaaaagaac
    27301 cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta gagtgcgttg
    27361 aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg tccgattcga
    27421 cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg
    27481 gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt gatttatata
    27541 ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat tctatctata
    27601 aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc ttccttgatg
    27661 taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct aaactgatcc
    27721 ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc gataacccgc
    27781 tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg tttgctgaat
    27841 ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact gacttttggg
    27901 gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct gtttatgatt
    27961 tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt actcagcttt
    28021 atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca ccaaaatcaa
    28081 aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa gaaatcgaga
    28141 tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa
    28201 cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca aatggcaaga
    28261 ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat ctagcagatg
    28321 ctttattgac cattttccac cagcattacc actaccagga tattcggcgt gtaggcgtga
    28381 actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa
    28441 agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa aaatacggct
    28501 ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc gcacgtagtt
    28561 ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa gatgagacgg
    28621 acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg attaaaatgg
    28681 gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga agaagaagca
    28741 cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag tatactgaca
    28801 gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga tgaatttgga
    28861 agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga ttactttatg
    28921 attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa ggaaacaaaa
    28981 aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa ataagaaagg
    29041 atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt cgaagaagca
    29101 acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt agttttgagt
    29161 aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt cggtaagatt
    29221 catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac cattcaacat
    29281 aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata agccatgctt
    29341 cagtcaattg attggactga agcactttat gctgaatttg cccttcatct acaagcagga
    29401 tcgataaagc tttgtcttca tctgattttt tcaatacact catttgaccg gcattctcta
    29461 ggataacatg tttggcattt tcaagaaaaa agcattcttg ctgtcttaag attgctcgca
    29521 actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg ccgtctttga
    29581 ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt tggacgatac
    29641 gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca tgccctacat
    29701 ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc gtatagacta
    29761 aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc actaataata
    29821 aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt tctcccccta
    29881 actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat atacaaggaa
    29941 gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc aaaagttaga
    30001 cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat atttttacac
    30061 aaccgttacg gttgttttac gaaat
//
LOCUS       DAHEYX010000007        29385 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHEYX010000007 DAHEYX010000000
VERSION     DAHEYX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.007"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..484
                     /locus_tag="MB219_000220"
     CDS             <1..484
                     /locus_tag="MB219_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCC8425958.1"
                     /translation="LLSKPRSGYELKQLITIFWEAHHSQIYTTLAKLDQQGYVTVLDK
                     DEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLDKTTATGLLFTR
                     EQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFFE
                     "
     gene            complement(683..1129)
                     /locus_tag="MB219_000221"
     CDS             complement(683..1129)
                     /locus_tag="MB219_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCC8425959.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(1176..2654)
                     /gene="msr(C)"
                     /locus_tag="MB219_000222"
     CDS             complement(1176..2654)
                     /gene="msr(C)"
                     /locus_tag="MB219_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCC8425960.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(3008..3637)
                     /locus_tag="MB219_000223"
     CDS             complement(3008..3637)
                     /locus_tag="MB219_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCC8425961.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(3621..4085)
                     /locus_tag="MB219_000224"
     CDS             complement(3621..4085)
                     /locus_tag="MB219_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCC8425962.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(4572..5840)
                     /locus_tag="MB219_000225"
     CDS             complement(4572..5840)
                     /locus_tag="MB219_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCC8425963.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(6071..6808)
                     /locus_tag="MB219_000226"
     CDS             complement(6071..6808)
                     /locus_tag="MB219_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8425964.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(6875..7444)
                     /locus_tag="MB219_000227"
     CDS             complement(6875..7444)
                     /locus_tag="MB219_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCC8425965.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            7572..8444
                     /locus_tag="MB219_000228"
     CDS             7572..8444
                     /locus_tag="MB219_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCC8425966.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            8786..9658
                     /locus_tag="MB219_000229"
     CDS             8786..9658
                     /locus_tag="MB219_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8425967.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            9655..11613
                     /locus_tag="MB219_000230"
     CDS             9655..11613
                     /locus_tag="MB219_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8425968.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(11864..12715)
                     /locus_tag="MB219_000231"
     CDS             complement(11864..12715)
                     /locus_tag="MB219_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8425969.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            12838..13305
                     /locus_tag="MB219_000232"
     CDS             12838..13305
                     /locus_tag="MB219_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCC8425970.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(13568..14050)
                     /locus_tag="MB219_000233"
     CDS             complement(13568..14050)
                     /locus_tag="MB219_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCC8425971.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(14150..15013)
                     /locus_tag="MB219_000234"
     CDS             complement(14150..15013)
                     /locus_tag="MB219_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCC8425972.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            15611..16459
                     /locus_tag="MB219_000235"
     CDS             15611..16459
                     /locus_tag="MB219_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425973.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(16630..16971)
                     /locus_tag="MB219_000236"
     CDS             complement(16630..16971)
                     /locus_tag="MB219_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HCC8425974.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            17057..17764
                     /locus_tag="MB219_000237"
     CDS             17057..17764
                     /locus_tag="MB219_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCC8425975.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            17751..18074
                     /locus_tag="MB219_000238"
     CDS             17751..18074
                     /locus_tag="MB219_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCC8425976.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(18163..19200)
                     /locus_tag="MB219_000239"
     CDS             complement(18163..19200)
                     /locus_tag="MB219_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCC8425977.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(19647..20630)
                     /locus_tag="MB219_000240"
     CDS             complement(19647..20630)
                     /locus_tag="MB219_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCC8425978.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(21046..21816)
                     /locus_tag="MB219_000241"
     CDS             complement(21046..21816)
                     /locus_tag="MB219_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8425979.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(21809..22744)
                     /locus_tag="MB219_000242"
     CDS             complement(21809..22744)
                     /locus_tag="MB219_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8425980.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(23338..24987)
                     /locus_tag="MB219_000243"
     CDS             complement(23338..24987)
                     /locus_tag="MB219_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCC8425981.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(25182..26078)
                     /locus_tag="MB219_000244"
     CDS             complement(25182..26078)
                     /locus_tag="MB219_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCC8425982.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(26071..26751)
                     /locus_tag="MB219_000245"
     CDS             complement(26071..26751)
                     /locus_tag="MB219_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8425983.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(26752..27534)
                     /locus_tag="MB219_000246"
     CDS             complement(26752..27534)
                     /locus_tag="MB219_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8425984.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(27527..28444)
                     /locus_tag="MB219_000247"
     CDS             complement(27527..28444)
                     /locus_tag="MB219_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8425985.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(28447..28731)
                     /locus_tag="MB219_000248"
     CDS             complement(28447..28731)
                     /locus_tag="MB219_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425986.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         8677 a         6159 c         5210 g         9339 t
ORIGIN      
        1 gcttttatcc aaaccacgtt ctgggtatga attgaagcag ctgatcacaa ttttttggga
       61 agcccatcac agtcagatct atacaacgct tgccaagtta gaccagcagg gttatgtgac
      121 cgttttggac aaagacgagc actctcaaaa aaagatctac cacttgactg atgaaggacg
      181 tgctcttgta gaggaatgga tcaaagagga aactcccgct cctacgcaaa aagatgaatt
      241 tctagcaaaa atctatgctt tttcgacgct cgataaaaca acagctactg gactgctatt
      301 cactagagaa caacagctga acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct
      361 ctcgctagac aagaaacaag aatttggacg ttatgttgtg atccagcgca gaattatgct
      421 ttgtcagcag gaactccaat ggtgtcagca agtaagagaa caaatgcagc agttttttga
      481 gtaagggata aacagaacct ttctagaaat atctattgaa gagcaaatta tcttcaataa
      541 aagagcaata cagttggaaa agaaaaagcg atccgaacga caggccaaaa cctatcgttc
      601 ggatcactat ttttctagaa ctgcattcaa gagactatga caaaaagcat gccaaggcct
      661 tttttcaaat aacagtgttc catcattcat tactttgaat tgcttttagc acacttcttg
      721 aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc aatcacattc attttccaag
      781 cattcatata agcagagaat aattgatcag gtattccttg ttccatgatc acaccgaata
      841 aagacataca tgtcaccatt cctaaaacca tcaagcatga aatagttaag atcatttgga
      901 ccttgctttc tggatttact ggtagtttaa aggcaatttt tttcgccagc acaccgatga
      961 tgaagacatc caaaatgaaa gccaccacga atccaggcac tagaccaatc aacaaacttg
     1021 ataaatgaag atcttgatgc agccataaat tatacgcgct catccccaag accatcaaaa
     1081 aacacatcat tgttgtaaaa attattcctt cttttttatt tgtaggcaat ctgtaccact
     1141 cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt aagtactttt
     1201 tttattttct gcccttttat ttcataaact tcatcagccg tacgctcgac aaagtaagaa
     1261 tcatgagaag taaacaaaat cgttcccgga tatatttgca ttagcttctc taaagcttcg
     1321 atcgttgcca tatcaataaa attagtcggt tcatctaaca gcaacacatt acttggctta
     1381 gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc cacttaatgt acataacggt
     1441 ttcgtcaaag tttcctgagc aaaccctaag tgattcagca aactacggac aagcgattct
     1501 gaaaactctg tttgtctcat gacaaaacga atgatggatt cttcagatgt catttgataa
     1561 cccttttgct gatatacttg aaagcttact ttcggagaga gaaggattcc ttctccttgt
     1621 ttgcggatat gttctaataa ggttgtctta cctgaaccat tttcaccgac aatcgcgatt
     1681 tttttaccta aagaaaactg aaaatcacca tttaccagta atgttctccc acttctttcc
     1741 aattggacat tttgtcccat gatgggatag cggctgtgga gttcaagaga ttttggtact
     1801 ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt
     1861 ctctctaatg ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag
     1921 gaagacaacc gatccggtct gatttgttgc tttttctttt tactggacac tttttccaac
     1981 ttttccgctt gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg
     2041 atcgactctg cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca
     2101 ctataattcc ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct
     2161 agctcatcta gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga
     2221 agctcttcaa tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga
     2281 agcatccctt gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct
     2341 ccgctccatt tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc
     2401 aaatttaggt cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc
     2461 tctgtttgga ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc
     2521 gatttaccga caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga
     2581 gaatcaatag atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact
     2641 gctaaatttt ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta
     2701 aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac
     2761 gaaataacta tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc
     2821 gaaaaaatgg acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt
     2881 tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc
     2941 ttttttatta ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga
     3001 aaacctttta ttcgaacgta gtgatctttt ctggtgctaa acgaacagac tcgtaacctt
     3061 cttctaccgc ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac
     3121 caaatgcctc tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat
     3181 gatcacgcgc aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat
     3241 cgttcatttc ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat
     3301 ctctgacttc ttttggcatc ttccctgatt cgtaagcttg gttatagatt tcttcgccat
     3361 attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc
     3421 tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca
     3481 caaagcgcca tggttgcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt
     3541 ccagcatttc ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac
     3601 cagtcataat tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct
     3661 gttgttatat tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt
     3721 tctacatcgc tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa
     3781 tgctcgattt tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga
     3841 ggatttctct gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact
     3901 gctccttgat caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct
     3961 ttcagtctca tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc
     4021 tggacgattt tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa
     4081 gacataattt cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa
     4141 taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa
     4201 agtatagaga aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac
     4261 gctaaaacca agattattga ttcttatcaa actatagaag atagctcttc tgcagtagaa
     4321 aattttacac cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag
     4381 aaattggatc agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcacaacc
     4441 ttttttgagt aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa
     4501 aaacatgaaa atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc
     4561 actacctctt tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt
     4621 agccaatctc tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga
     4681 ttaaatagtc gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc
     4741 caaccgattt ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca
     4801 ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc
     4861 ccttcatatt cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat
     4921 aaatgttctc ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct
     4981 gtatcatact gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta
     5041 ggcgcagtgg cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg
     5101 cttttccatg caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc
     5161 gttacgttat cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc
     5221 gtaatccatt gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag
     5281 cttcctttaa tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag
     5341 ccactttcaa cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc
     5401 aaactttgtg cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag
     5461 cgagcataaa aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta
     5521 tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg
     5581 ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt
     5641 ggttcttcgg ttgaaattgt attttctgag acagtagctg actgcaaagt actctctgta
     5701 tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat
     5761 tcttcagcta gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat
     5821 aataaattct ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac
     5881 agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt
     5941 ttgataaatt tgctttttct acataaaaag acgtaaatga agaatattct tctccattca
     6001 cgccttaaat tgatggtcgt ttgaataact atttaacaag taattctctt tggatattat
     6061 ccgttatgga ttatacttcc tgtgtcgtcg gacggataat catgttactg acagacatcc
     6121 ggtctggcgt ttctatcgca aatgctacag cagaagctat atcttcggag gtcaatcccc
     6181 attctttttg cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata
     6241 attcagtttg aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct
     6301 cttgacgtaa tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg
     6361 aatcaggata aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt
     6421 gtttttccat gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat
     6481 caagcatatt ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc
     6541 ctgcattatt gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg
     6601 cttggacttg tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta
     6661 aagattcttt tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc
     6721 ctttttctgc taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga
     6781 caatcacttt ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg
     6841 cttatactca ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga
     6901 cgattttggc agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg
     6961 cattcgctgc aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg
     7021 ctccgatcca agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt
     7081 ttggatcggt tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat
     7141 cttctaaaca gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt
     7201 tccctatact aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat
     7261 catctacttt gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt
     7321 acccagtatt cagcgctgct aaaggctctt cgttttcagc ttggatctga tggatcactt
     7381 caaagagcgg atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg
     7441 tcattttgct ccctcctttt atccttactt tataacggac ggcttatcat gtctaatact
     7501 tataaaacat acttaactat tccttttaac tatacttagt ggtatgcttt tttaaaaaca
     7561 ggagggaaaa aatggatatt cgtatactga cgtatttttt agcagtagct agagaaaaaa
     7621 atatcagccg agcagcaaaa tctctgcata tcacgcaacc tactctctca aaacaactga
     7681 aagatttaga agaagaactt ggtgtgacac ttttcactcg gggctcccga gagatacagt
     7741 tgacggattc tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa
     7801 aaacggcgaa caatttaaca aatgacgcaa tcatctctgg agaaatctac attggcggag
     7861 gcgaaactaa agcaatctct ttcatttctg atagccttca ttcgttgatc aaggcgcatc
     7921 ctgatatcca gtttcattta tatagcggga atgcagatga tatcatagac aaactggata
     7981 gcggattact tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat
     8041 ttcgactgcc agcccaagat cgttggggac ttttgataca tcgaaatcat cctttagcaa
     8101 agcaaaccgt catccatccg caggaactga aaactgttcc tttgatcatt tcccggcaaa
     8161 cgtcagtgga cagtcaacta gctgaatggc taggtttcag catagatcag ttatcgatcg
     8221 tcggaacata taacttattg tacaatgctt ctttgatggt cgataacggg acttttggtg
     8281 cattagctct agatgggatc atcaatactt atgggacgga aaaaatattc atcccactcg
     8341 atccgccatt acaagcttca ctgaatctca tttggaaaaa agaccagcct ctttctcctg
     8401 ctgctcgtaa atttttagat gtcctaacgg agaaatacag ttaagtagag tttttttcct
     8461 tcgctttcag ctttttaaca acgaataaga taagcaaaag atccaacgat taaataacat
     8521 aagtttctta atttatttct tccttttatt tctgataatt ccagtaaatt actataatta
     8581 taaaaaaaga attgacaaaa cataactgtc tatataaaat tgattacgtt aacaataatt
     8641 cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta
     8701 ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa
     8761 ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt
     8821 attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag
     8881 aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga
     8941 aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc
     9001 agaaacgatc attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt
     9061 gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat
     9121 ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca
     9181 atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg
     9241 atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat
     9301 ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga
     9361 tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct
     9421 tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt
     9481 caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa
     9541 agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg
     9601 tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa
     9661 gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct
     9721 gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat
     9781 tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg
     9841 ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca
     9901 tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa
     9961 ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc
    10021 ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta
    10081 tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc
    10141 agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa
    10201 ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg
    10261 atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc
    10321 aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa
    10381 gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga
    10441 aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga tgctagcagc agcttttgct
    10501 tcattcttta ctggtgtgac agaaccactt gaattttcat ttatgttcgt tgcttggcca
    10561 ttatatgtct tgcatgcaat tttcactggg atttctttag cagtgagtgc attcttccat
    10621 tggacagcag gatttgcctt tagtgccgga tttgtcgatt acttcctatc tttgaaaaat
    10681 ccgattgcga atcagccatt gatgttgatc gtccaaggat tagtaacagc agtgatttac
    10741 tactttggct ttaactttgc tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa
    10801 gcagatcttt cagatgacga tactgcaacg actaattcag acaataaata tgcggcacaa
    10861 gcaagcaaaa tctacgctgc attaggcgga gccgacaatg taacttcgat cgataactgt
    10921 actacacgcc ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa
    10981 gccactggcg tacctggtat gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt
    11041 acagaagtac aattcgttgc agatgagatg gcaaaactac atggtggtgc tgcagcacga
    11101 cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg ccggtgaaac gatcgaacaa
    11161 gatatttacg ctatcgcaaa tgggaaactg attccaatca ctgaagtttc agatgatgta
    11221 ttcgcggaaa aaatgatggg tgacggctat gctgtattac cagaaaatgg agaaatcttc
    11281 tctccaatcg ctggtacgat cacaaacatt ttcccaacga aacatgctgt cggaatccaa
    11341 acagatgctg gtatcgaagt cttgcttcat atgggcatca acactgttga cttaaaaggc
    11401 gaaccattca ctttatatgt agaagaaggt caaaaagtcg caagaggtca attgattgct
    11461 ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca cggatatggt cgttgtattc
    11521 acaaatggcg acaaagtcca aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat
    11581 gataaaatcg gctctgtttc caacaaagcc tgagccatcg tcttccccag acttttttat
    11641 cgataaacaa acaggagctg agacaaaaat cttgtctcag ctcctttttc cgaataaaaa
    11701 cggcgttctc ctgccaactc cgcggtaata agtccagcta aacgaaacat atgaagagct
    11761 attttttatt tgactcttct tattacttga tgcagaacag ctttttcaca aaatttttct
    11821 tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata
    11881 tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat
    11941 cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag
    12001 ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt
    12061 caataatttc taactcttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt
    12121 cagttagatt gccggataat tctaagattt ctggagagtc actttctact agtacttttg
    12181 ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat
    12241 aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc
    12301 aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt
    12361 ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga
    12421 tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat
    12481 ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg
    12541 agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc
    12601 ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga
    12661 attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg
    12721 ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt
    12781 tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg
    12841 aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa
    12901 gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat
    12961 gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa
    13021 ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc
    13081 atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt
    13141 ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa
    13201 gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa
    13261 gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc
    13321 ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc
    13381 cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct
    13441 tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg
    13501 aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc
    13561 tatattatca tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg
    13621 taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc
    13681 ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg
    13741 ctaaagcgct tacaacaaga tacgttcctt ttgggaatgg gatgattcgc gtagtgtttg
    13801 gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt
    13861 tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac
    13921 cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc
    13981 tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat
    14041 actttgccat ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg
    14101 gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac tatttttttg
    14161 ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa
    14221 aagagatctc gggagcttcg actaacccat aatctgaaga aggaagccat tctgcataga
    14281 ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc
    14341 aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag
    14401 caaagccaat catgtgcatc aaactgccct tttcttccaa gcggtctcca tcaaatccat
    14461 aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt
    14521 gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga
    14581 caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt
    14641 ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta
    14701 tgccgtatct actgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa
    14761 aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta
    14821 gaagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc
    14881 ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta
    14941 ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca
    15001 gatctttaag catatttatc ttcctccttg attgcctttt tatcatatta tcaaagaaag
    15061 tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct
    15121 aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt
    15181 tttaccttta gaaaaataaa ttttggggaa gacagattct aagtaaacaa agcggtaaaa
    15241 atatctttta ttcccttagt taatccgctt actacctact gtacaaagca aattgttgtt
    15301 caaactgctg atcagaacgc tgcttggatt tttctttgcg atacatcata cctcttttat
    15361 caattggaac aattatttat ttctaacaaa atcacttcat ttttgtcaga taaataaaaa
    15421 acaaaaaaag ttccatcttt aaaacacgct attcttttac tttttctcac attctctaag
    15481 aaaagaaaag gagaatgaat ttttgacaat tcgagcaaaa gtgattatca aatatgtaaa
    15541 atcagctact tttttcttaa acgtttcttt atactaaata taaataagtg tttcatgaaa
    15601 ggagaactag atgaataatt ggcttgagta ttttcctgaa aacgttttgg aacgcggtta
    15661 cagctatcat ctgcacggtt ttgtccgtca tttgaactat acaagcaagt acttgtccgc
    15721 aacagtttct ggcacggaag attataaggt agtgatcact tgggatgaaa agaccaacat
    15781 gacttgtgat tgtctatatg ccatagaagg aaaaaaatgc aaacacatgg ctgctgtctt
    15841 attcgcttac gaagaacgtc caataaaaaa atccaactac tcactttccg aactctcgtc
    15901 ccttgtttcc tccgctagtt cctctcttgt acgggagctt ttaacagaga ttcttataga
    15961 acatccgcac tttatcgaac gcttcaaagt taagatgcca tttcatgcaa taaattattc
    16021 agataaatta acaactatca tccataaata cgatcatatt atcaagaaaa acaaaaacag
    16081 gaaaacagcg aagttcatca tggagatgcg aaagttcatc caagaagcag ttgaatcgct
    16141 tattcagcaa aatgcctatc ttcccgcatt cgaattgatc aatgaagtga ttgctactct
    16201 cgaaacattc tattgggagc ctgaggatga acgaaccctt cttctgatag aggactgtta
    16261 ttatttatgg aaagaattat tagcagaagc acctcatgca gaaaaaaggc aaatgttctc
    16321 ttggttcgtc tgccaagtcg atcatactga cgcctcttat tcaaaacgct actctataaa
    16381 aatcctgaaa gaagactttc gggaaaaaga attttctaac caaaagaaaa aaatagataa
    16441 aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt aaacgataac
    16501 aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg aatcatgcaa
    16561 aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact attaggtacg
    16621 cgttttgttt tataagtcat gacaaatttc cttcgttgct ggatagattt tccggcattg
    16681 ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccgctg catctgtata
    16741 ttggataaaa ttctgacaac aactttcagg tgtgtcgaac caccctaacc ctagtgcagc
    16801 aatcattgtt gcgcctaatg caggtccttc ctccatactt aaacaataga ccgttgcatc
    16861 gaaaatatca gcttggattt gtagccatgc aagatttttc gcgcctcctc ccaccgaaac
    16921 aatacgtgta aatctgcgac ccgctagttc tttacgtttc aatcccacca ttgacaaatg
    16981 ctcattaaaa atttagaata ttcacaagtt taacgacaat tatgaaaatt tatgtcgtgc
    17041 taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt
    17101 acctaccgtt ttcggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc
    17161 cggatttcat ccactagtcg tcacgttgat gtccctgctg gtctatgctg gttctgccca
    17221 atttatcaca gtcagcatgc ttgctagtca cagcccattg ctttccatcg ttttctcgac
    17281 ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa
    17341 aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact
    17401 gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc
    17461 taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa
    17521 ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg
    17581 gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct
    17641 gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa gcaacttagt
    17701 gattgtagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt
    17761 ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt tctagggtcc
    17821 taccatttgt cttactgaaa aaattcgatt taccgcaacc gttgcttgag tatttaagtt
    17881 ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg
    17941 gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg
    18001 ctattctttc aaaaagctta ttggtgatcg tcttggcagg aattctttcc ctttcgttga
    18061 ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt
    18121 ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc
    18181 actaacaacg acttttccta acatatgtcc tgtttctacg tctttggttg cttgtttcaa
    18241 gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc
    18301 atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata
    18361 gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc
    18421 tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag
    18481 gtgcttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg
    18541 atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc
    18601 tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag
    18661 gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccggatc cgttgatcac
    18721 taagattttt ttcccgcgat tggcattttc ttctggagtt agaccaaatt tttcaaataa
    18781 caattcataa gcggtcaatg aggtcaacgg caaggctgca gcttcttcat ctgaaagatt
    18841 ttttggtgca agagcgacaa tacgttcgtc aactagttgg tattcttgat tgcttcctgc
    18901 tcttgttgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcgtc
    18961 acctacagca acaactttcc cgactccatc aaaacctaag atacgtaaag catttcttat
    19021 cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac
    19081 cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg
    19141 atcatcgatc ggcaaccctt cataaaaacc gactaccttc attaattcct tttctgacat
    19201 acgaattcct ccttagcttc atcaagttgg agcatccata cagtgttcat ttcaatatat
    19261 atagtatgct atttgttcca ccttttttat tcatcataat tattctctta cttaaagtaa
    19321 actatttgca cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt
    19381 ataaacggtg ttctcctacc aactcttcgg tcacaagtca aaatattcat caaacatgag
    19441 aagctgtttt ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct
    19501 gatataaacg gtgttcaatc aactgaccct cggtattagc tcaaaaaacg acaaaatatg
    19561 aagagctatt tttgtttggc tgttcttatt acttgataca ggacagcttt ttcacgacct
    19621 ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg atgttctttt
    19681 tcaaaggtct ctaagatctc tcgtacttca tcagtagatt tcgtattcat caactgaact
    19741 cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg atgtagtcct
    19801 gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg cagacgaagt
    19861 agtccgatca gttctttttc tgtatgttcc ctcgggtctt tttcaaatgc ataaggattc
    19921 ttgaaaatcc ctcggccaat catgatgcca tcaacgccat atttctcggc caattcttct
    19981 cctgtctggc gatcggggat atctccattg atcgtgatta gtgtttgtgg agcgatttgg
    20041 tcacgtaaag caatgatttt aggaatgact tcccaatgtg catctacttt actcatctct
    20101 ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag gtgactgatc
    20161 cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa
    20221 ccgccagctt tagctgcttc aatcaattgt gccgctactt ctggacgaag gatcaagcca
    20281 ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat atcgattcct
    20341 tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg cttgtctccc
    20401 caaatatggg caaccatcgg ctgttcatct tctgtaaatg ttaatcttcc tcgtacactg
    20461 tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa gaagacatcc
    20521 ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc ttccattggt
    20581 gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat ttatttgtta
    20641 ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt cattatacta
    20701 ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa
    20761 agactgtgac aaaaatctta tcacagtcca ttttccggaa taaatggtgt tcaaaagctt
    20821 gcatttgcgg taataagtcc aaccaaacga aaaataggag gagctatttt tgtttggctg
    20881 ttcttattac ttgatgcaga acagcttttt cacaacctct ccactaaacg gtgttcaatc
    20941 agctgaccct cggtattcgc tcaaaaaaca acaaaatatg aggaactatt ttcccgtttg
    21001 tttctctaat actcaggtca aaacgctgat ttcacaacct cttttctaac ttctcaatcc
    21061 tcttccttta ttgatcaaat accaagtaac ggcgtacaaa acaacgatga ataacacaag
    21121 tacgatcatc gatgttgcta ctggcacatc gatcgttcca aggaatccgt aacggaaacc
    21181 ggagatcata taaacgatcg gattgacttt tgaaacagct tgccaaaagg gcggtaacat
    21241 agagatggca tagaaaacgc cacctagata agttaacggc tgcaagacaa atgtaggcac
    21301 gatcgataca tcatcataag actgagcaaa aatcccgttg agcagtcctg ctaatgaaaa
    21361 caagattgcc gtcatcaata atgtaatgat gaccatgaac catgaataca catgaagtgg
    21421 tacaaagaac aaggaaatga tcgttacgag agagccgacg agaatactgc gacccacacc
    21481 accaatcaca aagccccaaa taatcacatg cgtaggaaca ggtgctacca agatttcttc
    21541 gatatttttc tgaaattttt gagagaagaa ggacgaagaa acattggcat acgaactagt
    21601 gatagcagac atcataatca aaccaggaac gatgaattcc atatatgaaa aaccacccat
    21661 gtcaccgatc cgcccaccga tcatttttcc gaaaataaca aaatacaagg atgtcgtgat
    21721 cactggcgga accagtgttt ggacccatat acgcaaatag cggttcgttt ccttgatagc
    21781 taaacttttt aatgcagtaa aataaagatt aaacattttt ctcctccgta tgattatttt
    21841 cttctgtgat tttcaagaac agttcttcta aacgattcga cttattcctc atagaaagaa
    21901 ctttgattcc ttgtcttgta agctgatcga agattccgtt caccccttga tttcgttcaa
    21961 cttcgacagc gattgtctgc tcatcttcaa atgtacaagc atatccttca atcacaggtt
    22021 tggtatcata tggcgctaaa tcaaagataa atgtttcaaa ttgcagtttt gacagaagct
    22081 ctttcatact tgtattctcg atcagttcac ccgattggat gattccgatg ttgcgacaaa
    22141 gcatttcagc ttcttctaga taatgcgtcg tcaaaatgat cgttgtacca ttttcgttca
    22201 attctcgtag aaactcccac atctcgcgtc tcaactcgat gtcaactcct gctgttggtt
    22261 catccaagat cagcaaacgt ggttcatgca tcaatgccct agcaatcatc agtcttcgct
    22321 tcattccccc ggaaagcata cgtgcacgga catttctctt ttcccaaaga ttggactgct
    22381 tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga gacaccgtag tagcctgctt
    22441 gattcacgac gatttgctgg accgtttcaa aagggttaaa attgaactct tgtggaacca
    22501 atccaatctg ttgctttgct cgaactagat ccttgtccag atcataaccg aagacattca
    22561 cttttccaga tgttttattg actaaagaag taataatccc gatcgttgta gacttcccag
    22621 ctccatttgg ccctaatagc gcatagaaat ctccttcttt caccgaaaga tcgattcccc
    22681 gcaatgcttc gaccccactt ccatagatct ttttcaaatt atttatttca agcgcatttg
    22741 ccatttattt cctatctcct tatagacatg attcttaatt gtatcatgga atacagcaga
    22801 tgaaaaaaca acttggcttc cacacaaaaa cacacctgct aacaaagagg agatgtgttt
    22861 tctagttcat gatcatcttc gatagaataa ctatctaaac aactctggaa cttttaaaat
    22921 cataaaagta ccgacagcaa ctactacgat gacatataga agatccttat aactgaggtt
    22981 cttgtctttt ttattcatgt taagctttcc tccgtccttt tatcgaaagc ttttttcttt
    23041 tattttaacc gaaataaata aaatggaaat agttattttc aatttctgtt ttcgctgttt
    23101 gttttacgga gaactttgcc atggaacgtg cgtctcgtcg ctgatgctaa gttttttcga
    23161 taaaagctgc tagaaggaag acagcgtggt ttattttgta tcgtcttttg ctaaatcttc
    23221 caagatcatc tggacagcag actcgttccc attgaattct gccacttttt tggcatcaat
    23281 gtacttcttt tgataatcgt ctttttggac ccacgcatca tgcttcgttt gataaagtta
    23341 atcaaacgga taccactgac gctgttcatc gaacaacaaa ctttcttttt gggcctgttc
    23401 aactatggat ttaggataat tcaaaacttt cagcaaggca atcgcatttc gggtccttga
    23461 aggtccttct tttaatttga aatcaaaact gattcctttt ccttctgtta cttgttcttc
    23521 aaagtggaca ttctcacaat aattctttaa aatctctgtc aattcgatat catgtgtggc
    23581 gacaaatgct aaagatggat actccgcaag ccaccgaacg actgaagcgg aagaagcaat
    23641 ccgttcgatc gtatttgttc cttttaaaat ctcatctacg aagcagtagc agcgttcttt
    23701 ggaagccact tggtccagca aacgtttgat cgatttgatt tctgaaacaa aataactgtc
    23761 gccttcaaat aaatcatctt cgattgccat ggaagtcaac acatgtccag gttgcagggt
    23821 aaactgttct gcaatcgctg tttggatcgt ctgtgctaga atacagctga tggctatact
    23881 tttgacatac gtagattttc ctgatgcatt ggaacccgtg actaatgtat tcttctgcca
    23941 gttgactgga ttaactaccg cttcttttaa caatggatga tagatatctt tcgctgtcac
    24001 accgccctca gtaaactctg gtcggcaagt gatcggcata taggtgcgat aattcaaaac
    24061 ggctgctgca acttccaatt ctcccagcaa ttcccacagc ttgatggctt cagttgaatg
    24121 cttttctaat ttagtgagaa cgaaatgata ggagataaat ggcagcatga acatgatgtt
    24181 gacatattca aaaatcaatt ctgcttcact cccgctcttc attcgaaatg aaaaacctaa
    24241 tttagggatg tctcttaatg gtttagtttg ctgtttcagc tctttttgaa gtggtgtcgt
    24301 catttttgct aactgattgg cggctgaaat cgttgaaacc aaataactca tgctattcag
    24361 ctctgtttct agtgtttgtt tcttcactag ataatagacc gtattgaata ctaagccacc
    24421 gattgctaga tagattccta gtacttgccc taaaagaagc aaacctacac ccaccagcgg
    24481 aagcaagcca agaaaaataa ataaacctac gcgtccaatc tttttacttt ttccatccgc
    24541 tagatattgt tttgcaaaat tatgatcttt ttttcctaaa cgagcgaatt gatattggat
    24601 tctttctctc agttgaggat tttcctgata aaaagcaatt agtttttcta actgataatc
    24661 ttctccaaaa tcaaagctac gtaaacgctg atacaacgct tctgaaccta cactagaata
    24721 ggtactgttg atcccttcaa atacttcgaa catatccaag tcataccaag taagatcgtc
    24781 gatttcacta tcccatgaac ggaatttttt ctccgtgagc cacgcatcct tcaaactttc
    24841 ttctttatca aatctcggtt gataaggaat ttttccccaa ttactacgaa ctgtttcctt
    24901 tactttcaaa cgtgaaaaaa tgtctaaaac aactaataaa ataacaactg caataataat
    24961 taaaacgata aattctcttt ggcccatatc cctctccgtt ccttgatttg ttagttttag
    25021 tatagcactg ttgcgctatt tgaaaagggg gtacaaaaat aactgtcttc gttacctaat
    25081 tcgtatcaat cacaccaaaa taaaaacacc ttccaaaaaa gaagatgtct ttaaaacgag
    25141 atacaatttt ttgtcattca cttcaggtaa attagagcca attaaaccct ccgcaacttg
    25201 acggagatgg agaagatgcc cccgtctaat ctagcggtca tctctcctcc cattccttcc
    25261 accaattttt ttgacaggta aagcccgatt ccgctagatt ccgtatttgt gcggctggta
    25321 ttttcggtat aaaaacgctg gaagactttt tcaacagagg tttgcgctcc tggtttgata
    25381 ggattggcca ctgttaatcg aatgaatgaa tcctcttttg aaaatgtcac ctttgcttgt
    25441 ccatctgcat aacgcaataa attctgtatg atattttgca gcactcgcag caacatgagc
    25501 ggatctgcta atacagttgt atttatcgag tgttcagcga acgctacctc gatatttttc
    25561 tcagaaaatt gttcataaaa agagaaaaat acttcttcca cctctttaga aaaagaaagc
    25621 gtatggagat ccatttcttt ttgcttcgtc tctattcgag caagatcata aaactgatcc
    25681 gtcatactga gtaatcttgc aacagattgt ttgatgatcg ttaaatactt ctcccgtttc
    25741 tccgtatcct ctacctgttc cagtaattgt acataaccgt taatagcagt caaaggagtt
    25801 ctgagatcat gagaaaggcc gacgatcgat agtctgatgt tttcttccat ttcttgaaat
    25861 gcttgtcctt tttgttcatt ttcatccacc catgtattca gtgcatctac taggtccatt
    25921 agattctttt ctcgaaattc gactgaaaga cgaccaccat attcttgtcg ttcagaaaga
    25981 gtggcgattt tcttagttaa tctcttgatt tccctttgca ggaagaaaat aaaacttccc
    26041 ataaagataa caacgagaag caacagccac ttaatcaaat ctaaacccca ctccccacac
    26101 cgtatgaata tacacagcat gtgcctttgc tagtttccct cgaagattgg aaatatgcat
    26161 attcactgtt ttctcactat caaaatatgg ttcttgccaa actgtttcat aaagatttgc
    26221 tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc agcagatcga attctcttcc
    26281 tgtcaaatgg acagccacac cattcacatt tacatccctc gactctttgt ccagtaagat
    26341 ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg acagaagtct gatgacgaag
    26401 ttggatgccg attcgtaaca acagttcttc gatatcaaat ggtttcgtca catagtcatc
    26461 cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat
    26521 gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg
    26581 aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc
    26641 cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaaaga
    26701 atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggaaca cctacttaat
    26761 gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg ttactagact
    26821 gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat
    26881 gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac
    26941 accgggaacg aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt
    27001 acttctagtg atgaatgtca aagccactgg aacagataat gtcgctaata tcagcagaag
    27061 caatacagga aatactttta gaacataatt tccatagttt ttgattccct caacttcacc
    27121 aaatccgaaa gcaagtgtgt aagcaatcat ggctagcccc gtaatcaaaa ccacgccaag
    27181 tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc
    27241 taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc
    27301 aaatgctgcg aaagccagaa gaatccatgc agccattttt tccaccatta aaatccctgt
    27361 tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat
    27421 ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca
    27481 cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat
    27541 tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg tggataccat
    27601 ccacaggaac ttttgcctct accaatgctt gattgatggc tgctacttgg tcaattcctt
    27661 caaaaagctg gataacacgg ctagattgga caaaataatc cctataacca agagagtcga
    27721 gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt
    27781 gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccgtagt
    27841 gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc
    27901 gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc
    27961 cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag
    28021 ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca
    28081 atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt
    28141 ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt
    28201 gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt
    28261 catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccattct
    28321 ccccaatcaa accatagatc attccacgtt tgatttcgat cgatacttta tctaatgctt
    28381 tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat
    28441 ccatacttat ccttctttct tattgataag tgaagtatag ttgacgggta taaagaaagc
    28501 agccagaaat gtataaagaa agtgtaaatt ttccaattga atttcaagaa aaatcagact
    28561 gtctcgcaga cgtggttgag ttgtctgcca atcaaagagc gcaccaaaca gtgctaatcc
    28621 aagtggactg gccaccatgg taatcgttgt tatcgttgac atcagacgtc cttgtatatg
    28681 cgttggaact tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta
    28741 aaaacggact tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa
    28801 gaggttatga caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc
    28861 aactcctcgg tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc
    28921 ttcttatttc ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg
    28981 tttgatgata ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag
    29041 aatcctacct attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt
    29101 gccagaaaag ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc
    29161 gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg
    29221 ggctttctta agacctggtt aaacaaaact ctattttatt gcattttaca ggaatgtatt
    29281 tcatgcgaaa ttaataagtc tttgtttata gagttttact agggtataaa aagttgtatg
    29341 ttatcattac ctcaaaacat tacctcagct tggcgagttg gttga
//
LOCUS       DAHEYX010000008        28977 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHEYX010000008 DAHEYX010000000
VERSION     DAHEYX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.008"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            108..1853
                     /locus_tag="MB219_000249"
     CDS             108..1853
                     /locus_tag="MB219_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCC8425987.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(2133..2570)
                     /locus_tag="MB219_000250"
     CDS             complement(2133..2570)
                     /locus_tag="MB219_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCC8425988.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(2554..3927)
                     /locus_tag="MB219_000251"
     CDS             complement(2554..3927)
                     /locus_tag="MB219_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8425989.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(4249..4737)
                     /locus_tag="MB219_000252"
     CDS             complement(4249..4737)
                     /locus_tag="MB219_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8425990.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(4759..5361)
                     /locus_tag="MB219_000253"
     CDS             complement(4759..5361)
                     /locus_tag="MB219_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCC8425991.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(5865..6494)
                     /gene="udk"
                     /locus_tag="MB219_000254"
     CDS             complement(5865..6494)
                     /gene="udk"
                     /locus_tag="MB219_000254"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCC8425992.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(6578..7273)
                     /locus_tag="MB219_000255"
     CDS             complement(6578..7273)
                     /locus_tag="MB219_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCC8425993.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(7286..8002)
                     /locus_tag="MB219_000256"
     CDS             complement(7286..8002)
                     /locus_tag="MB219_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCC8425994.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            8332..8937
                     /locus_tag="MB219_000257"
     CDS             8332..8937
                     /locus_tag="MB219_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCC8425995.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            9262..9585
                     /locus_tag="MB219_000258"
     CDS             9262..9585
                     /locus_tag="MB219_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCC8425996.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            9767..11137
                     /locus_tag="MB219_000259"
     CDS             9767..11137
                     /locus_tag="MB219_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCC8425997.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(11267..12568)
                     /locus_tag="MB219_000260"
     CDS             complement(11267..12568)
                     /locus_tag="MB219_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCC8425998.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(12798..14756)
                     /locus_tag="MB219_000261"
     CDS             complement(12798..14756)
                     /locus_tag="MB219_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCC8425999.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(14933..15199)
                     /locus_tag="MB219_000262"
     CDS             complement(14933..15199)
                     /locus_tag="MB219_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCC8426000.1"
                     /translation="MLNYLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(15709..17835)
                     /gene="pnp"
                     /locus_tag="MB219_000263"
     CDS             complement(15709..17835)
                     /gene="pnp"
                     /locus_tag="MB219_000263"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCC8426001.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(18023..18292)
                     /gene="rpsO"
                     /locus_tag="MB219_000264"
     CDS             complement(18023..18292)
                     /gene="rpsO"
                     /locus_tag="MB219_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCC8426002.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18543..19106
                     /locus_tag="MB219_000265"
     CDS             18543..19106
                     /locus_tag="MB219_000265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCC8426003.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(19359..19880)
                     /locus_tag="MB219_000266"
     CDS             complement(19359..19880)
                     /locus_tag="MB219_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCC8426004.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(19883..20725)
                     /locus_tag="MB219_000267"
     CDS             complement(19883..20725)
                     /locus_tag="MB219_000267"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCC8426005.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(20718..21443)
                     /locus_tag="MB219_000268"
     CDS             complement(20718..21443)
                     /locus_tag="MB219_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCC8426006.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            21619..21915
                     /locus_tag="MB219_000269"
     CDS             21619..21915
                     /locus_tag="MB219_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426007.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            22483..24075
                     /locus_tag="MB219_000270"
     CDS             22483..24075
                     /locus_tag="MB219_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCC8426008.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            24088..24828
                     /locus_tag="MB219_000271"
     CDS             24088..24828
                     /locus_tag="MB219_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCC8426009.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            24996..26345
                     /locus_tag="MB219_000272"
     CDS             24996..26345
                     /locus_tag="MB219_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8426010.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(26473..27063)
                     /locus_tag="MB219_000273"
     CDS             complement(26473..27063)
                     /locus_tag="MB219_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCC8426011.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            27210..28562
                     /gene="brnQ"
                     /locus_tag="MB219_000274"
     CDS             27210..28562
                     /gene="brnQ"
                     /locus_tag="MB219_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCC8426012.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         8525 a         5889 c         5432 g         9131 t
ORIGIN      
        1 aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt
       61 gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa
      121 atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa
      181 actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca
      241 ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca
      301 ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa
      361 agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat
      421 cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg
      481 cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac
      541 tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa
      601 aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa
      661 gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca
      721 agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa
      781 acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa
      841 acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt
      901 ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa
      961 caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg
     1021 gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca
     1081 actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac
     1141 aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac
     1201 tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc
     1261 ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg
     1321 tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga
     1381 aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg
     1441 aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta gaagatacat
     1501 tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac
     1561 agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga
     1621 aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta
     1681 tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa
     1741 acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta
     1801 tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg
     1861 ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca
     1921 aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa
     1981 aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa
     2041 cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt
     2101 cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga
     2161 cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct
     2221 tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca
     2281 aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa
     2341 ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc
     2401 gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc
     2461 gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt
     2521 atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc
     2581 cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca
     2641 ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat
     2701 caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa
     2761 taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg
     2821 atccattgga atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac
     2881 tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac
     2941 caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt
     3001 caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat
     3061 aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa
     3121 aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt
     3181 ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc
     3241 ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa
     3301 caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt
     3361 gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac
     3421 gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat
     3481 cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt
     3541 gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc
     3601 tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa
     3661 tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact
     3721 atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa
     3781 cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag
     3841 ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg
     3901 cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat
     3961 ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt
     4021 aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact
     4081 caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga
     4141 taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga
     4201 gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc
     4261 ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc
     4321 tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac
     4381 tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac
     4441 tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat
     4501 ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa
     4561 aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt
     4621 cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaagcttccg gccatacttc
     4681 ttgtatgact gccatcagct catccagttc attttctgtt acttttattt tttccataca
     4741 cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat
     4801 catgctatac acacccaccg accatggttt caagaaaatc acaatctgca taaattccct
     4861 aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag
     4921 cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc
     4981 aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg
     5041 gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc
     5101 aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg
     5161 aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg
     5221 ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag
     5281 aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa
     5341 aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc
     5401 tccttagggg gtgcgacaaa agagcttctt caaaaaaata agtcgaaatc tcacacaaat
     5461 tcgagaaata attttcgtaa gtttcttctt atttattgca tctgaccact tctgttacaa
     5521 cctctcgtta aacggtgttc aaaagcttgc atctacgata ataagcccaa cccaacgaaa
     5581 aacgtgagga gctgttttat ttggatgctc ttatcgcttg atgcaaagac agctttttta
     5641 caacctctac ttcacggtgc tcaatcagct gaccttcggt attagctcaa aaaacggtaa
     5701 aatataaaca gctattttcc gtttcttctt tttaatactt aggtcaagat gctgttttca
     5761 caacctcttg ttataaataa aaaaacagtt ggctggtgac tgttcaaaaa gcagaatcct
     5821 tgcttttcaa acggtcgcag tccgttcaac tgtttcgctg cgcactattt atggtttaag
     5881 aaactagcta cttttgttgt aatcaaatca atagcaacgt ggttttctcc gccttctgga
     5941 acaatgatat ccgcatagcg cttcgttggt tcgataaatt gatgatacat cggtttaaca
     6001 actgtcaaat attgttcgat cacggagtcc aatgtgcgtc cccgttcttc catatcccgt
     6061 ttgatacgac gaatgatacg aatgtcatca tctgtatcta cgtatacttt gatatccatc
     6121 aagtcacgca gacgttcgtc ttctaaaatc aggattcctt ctagaatgat cacttctttt
     6181 ggttcctgaa tgatcgttgc ctgacttctt gtatgcgcta catagtcata cacgggtttt
     6241 tcaatggctt tatagttcag taagtcgcct acatgctgaa tcagcagatc attatcaaag
     6301 gcaaatgggt gatcgtaatt tgtgttcaat cgttcttcaa aactcaaatg gctctgatcc
     6361 ttgtaataag aatcttgttc aagcatcatg atggaatggt ccgggaagtt gttgaaaatc
     6421 gcacggctga cacttgtctt tccgcttccg gatccacccg tcacaccaat gataattggc
     6481 ttgctttttg tcattgacta aaacctcttt cgtatccgtt ttttcattct aatctattat
     6541 agcgaaaaaa cacgaaaatg ctatattttt ctgaaaatta atccttttca atttccaata
     6601 atgaataaag ttcctctaat gaatcgattc gataagtagg ctggatttct tcgatttctg
     6661 gtaatttatt tctattgaac caaatcgtat cgatccctgc catatttcct cctttgatat
     6721 cagaagtcaa agaatcacca atgatcacag tttgcttttt atcaaaatta ggaatgtggg
     6781 aaaagacatg atcaaaaaat tctttcatcg gcttttgcgc tccgacagct tctgagacaa
     6841 agatatcctt gaagtaatca agcattcctg catctgttaa tcgccgatgt tgcgtgtgcg
     6901 acacaccgtt cgtcacaata taaagatcag catgcggatg gacctttttc aggatttcta
     6961 gacttcctgg aacaagatca tgaccttggt tcaagtagtg gcggtagcgt ttttccattt
     7021 gatcactatc tactgttttc ccaaattgct ggaaaagtaa cccaaaacga ctgcctatta
     7081 tttcttctcg tgatagtctg cctttttcaa actcctgcca taaatgatga ttcaaagcct
     7141 tatagctggt ttcgatttct ggtgtcaatg tgattccttg ttctgcaaac aaagcactta
     7201 aagcattggc ttcagttaat tgaaaatcta acaatgtatc atctacatcg aacaataaag
     7261 ttttatattt catctttttg acctcctatt taaaataaat acttaacttt ttcatctctg
     7321 ctaatgtcaa cgatcgatac tcgcctactc ctagctgttc atctaattcc aatggtccca
     7381 tgcttagccg tttcaatgcg gtcactttct ttccttgggc taagaacatc tttttgactt
     7441 gatggaattt tccttcttgg atcgtcaata aaaccgtact ttggtgcttg tctgctttga
     7501 gtatttttaa atcagccggc ttacattgat acccatcagc gaatacgatt ccattttcaa
     7561 atgctgttac gtctgccggt gtcacccttt cgttcacaac tacttgataa gtctttgaaa
     7621 cctttttttc tggaagtagc atttcatatc ccaattgtcc attcgaagtg atcagcaata
     7681 gtcctgttgt atctctatct agtcttccca ctggatataa tggttggaaa cgatcggcac
     7741 ttgcaatcag atcgattact gtcgtctttt ctggatctga tacagctgta acagcttttt
     7801 caggtttatt gagaagataa taggactcat ttgtttctaa gtgttcacct gctacttcga
     7861 tttgatgcag taaactatct acatttcgat tttgtcgaaa ttcgactgtg ccatctatca
     7921 tcacttgctt caaagcaaac agccgcttca tttccttgcg tgtcgtttct aatttttctt
     7981 ctattaattt atctaaacgc atgctgtttc ctttcttatt actactaatt aattatacct
     8041 tgaatattct tgcttgcaaa tacatcatca tagttattcc ttgacttaat taacagttca
     8101 cgttacggtt aaatagtaaa gtgaattgga tttctcatac acagcgtaat gacctggtgg
     8161 agaaaagttg aatcctgact ttctaagggg agatgcgcgc atctctcctt ttgcgctttt
     8221 ttaagcgccc tgccttcgat gatgtgcacg agattagcct ttagaattca cttaaataaa
     8281 atctgtccgt taccaaagca gacagtcaca ttttaaggag gaattttggt tatgtcagta
     8341 tcattagaag taagtaaaag agaagtacgt ccccgttcat taagaaacaa acttcgtcac
     8401 gaagggaaag ttccagctgt tgtttatggg tataaagtag aaagtacccc tatctcattc
     8461 gatggtttga cactaagcaa attgttgcgt gaacatggag cgaacacagt catcacgatg
     8521 gacatcgatg gacaaaaagt caatacattg gttcacaaga tccaaacgaa cacttttacc
     8581 aatcatttcg aacatgcaga atttctatct gtaaacatgt cggaagaaac agaagtcgaa
     8641 acagaagtcg ttcttgttgg cgaatcagtg ggtgctaaat ctggtggagt gttagctcaa
     8701 aacttgtata cagtagtcgt atctgctaca ccagacaaat tgcctgaacg aattgaagtt
     8761 gacgtgacaa acttggaatt aggtgattct attacagtag ccgatcttcc aaaaaacgac
     8821 gattacaaat tcgtgaccga cagtgaagag caaatcgctg ctgtagtcgc tcaaagtgaa
     8881 gaagaaagca caggtgaagg tggcgaacca gaagtgatcg gttcaacaga agaataatac
     8941 tgaaacaatc aggtggtgtt agaagcggcg ggtcagcagc ttttaacacg ccttttttat
     9001 ttctaatcgt ttattttcat atggggcttc cacttttgta aatggcttta caaacgaaaa
     9061 atcctaattt accaagcttt tcctcgcata cttttatcgt acctttataa aaatttatac
     9121 tttgaaaaaa agtattcaat tattgttgca tttttcctga ttctttatta gaatgaaccc
     9181 tcgttgaagt aagtatttct agtttatcct aggattaatt ataaaaataa aacattcgtt
     9241 gattcgaagg aggttaaaca gatgtttgaa agttttgata gcagccgcaa ccgttacgct
     9301 tcagttggtg tcgtatcaag tttgccagat gaattgattg acagtatatg gtttattatt
     9361 gacctagatc ttaagggagt cattccatta gaaaatatat taacctttga tttgatcgaa
     9421 aatcgtggaa aagtgacgat gcatttttct caagcaggaa gcaatgtgga aatggatatt
     9481 gatttgccgt ttgcctattc gaaccaattt cctgctcaag tatacgccta tgatgacggt
     9541 acgagagaaa cgatcctttt accaagtgag attagacaat attaaggtga tagataaacg
     9601 tggatgtccc ttattttaca gaccaggcca agttgttgat tctttgactc aattcttggc
     9661 ttctttttat ggagtttcat aaattatcat tgatttctaa ttggtattct caggataaac
     9721 gctatatact actaactaaa ttttaaagat ataaggagtt taaaagatgc gcgatttaac
     9781 aaaaggaagc cccgctaaac tgattttgat gttcactgtt cctttactga tcggaaatgt
     9841 ttttcagcag ttctacaata tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa
     9901 tgcgctggca gcggttggtg caactggaag cctgacattt ttgatcattg gctttgccca
     9961 aggtttaaca gctggtttag ccattattac cgcacaacgt tacggtgcaa aagattatcg
    10021 gggtctgaag aaaagttttg ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt
    10081 aactgtccta agtctcctgt ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga
    10141 aatacttgat caagcacaaa catttatttc aattatttta ttagggatct ttgcatcagt
    10201 cagtttcaat ttgctttcta atgtcattcg cgcacttggt gacagccgca caccactatt
    10261 ctttctgatc attgcggtca tcatcaacgt cgtacttgat ttgatcttta ttatctattt
    10321 tggcatgggt gtggaaggtg cagctattgc tacagtaatc gcacaagttt cttccagcgt
    10381 actgtgtcta gtgtatatca agaaaaaaat gcctttgtta cagttacgca aaaaagattt
    10441 caaatttgaa aaagaagagt ttgctgtaca cctaaatgct gctttgccaa tggcgtttca
    10501 atcttcgatc atcgctgttg gtgctatcgt gttacaagca gcactaaata gtctagggac
    10561 agatgtcgta gctgctcaag ccgctgctgg tcgtatcgac caatttgcaa atcagccgat
    10621 gatgtcattt ggtattgcaa tggcaacttt cactgcacaa aactatggtg cgaaagaata
    10681 tggccgaatc ttgcaagggg tacgtcaaac tttactgatg agtgtcggat ttagtctctt
    10741 agcaggtgct atcgtcattt tcttcggtca tttctttgtt caattgtttg ttagcccatc
    10801 agaaacacgt gtatttgaat tagcacaaac ctacttcaat atcaatgggt ctctttactg
    10861 gatcttagca gtcctgttca ttttacgcta cacacttcaa gggcttggac aaagcaagat
    10921 ccctacgatc gccggtatga tggaattgct tatgcgttct tttgcagcta tcgttcttac
    10981 aggaatgtgg ggatacccag gagcagcagc agcttctcct ttagcttgga tcggttctgt
    11041 tgccgtgttg ctttactctt atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga
    11101 ggaacatttc acattagaac atgcagaagc aagatagcct tgattaaaag aaatcgttgc
    11161 ggctaaaaat gaaaactttt attgaaaata tcttcttcgt ttaccgaaaa caggtttttt
    11221 agataacaaa aaggctgtga caaaaaatgt cacagccttt tttcttttat tctatttttc
    11281 tttccgtgga ctctactttg aaataatcga tatgtgcatg ctggtattct aataccgtga
    11341 aacggaatga tgcaatctca atgattgttc ctgcttttat ttctttatat cggctagaag
    11401 caaagcccgc aagtgtagta aaattcgttt cttcaaactc tggaatctct acatgaaaat
    11461 aacgctggaa atcatctaat gttagtttgc cggagatgat ataagagcca tctgactgtt
    11521 ccgctatcaa tgcttcgtcc aaatctgtct catctcggat ttccccgacg atttcttcta
    11581 aaatatcttc aatcgtcact aagccgcttg tccctccgta ttcatctttt aagatagcaa
    11641 tatgctttcc cttttttcgc atgatcgtca ataatttttt tatagggatc gtttcagttg
    11701 ttacgatcgg ctcttcaatc aactgttcta ctgtcagctg gtcatcttta taagattgct
    11761 tgatcaaatc tggcaaggtg acataaccta aaacattgtc ttttgattct aagataaccg
    11821 gatagcgagt atgtccttgt tcaatcgaag cttttatcgc ttctttgacg gtttctgaag
    11881 cttcgaatac ttgcatggac gtacggtcta cttgtatatc ttttgccaag gtatcatcaa
    11941 attcaaacac gttttccaaa taatgatatt cttctttggt caactctcct ttttcataag
    12001 aattcaatgc gactaatgtc agctcttctt gagacaatgt ctcttctcct tcagatacga
    12061 gacgcacacc taatagcttc cctagccctg cagctgaatg attcaatacc caaataaaag
    12121 gaaacattac tttataaaaa taatgaagtg gtttaactac gaacaagacg accttctccg
    12181 tattgacgat actatagctc ttaggaagca gttcgccaat aaccacatcg atatacgtaa
    12241 ctaaaataaa tcccagcgcc acagagattg tactgctaat agccgaacta aaaggtagtt
    12301 taccgatcac tggttctagt aaagccgcga cagatccttc accgacccac ccgatgatca
    12361 aaccggttag cgtaattcct aattgactag ctgataaata gttatctaaa tggtgtgtaa
    12421 catgaagagc tagcttggcg ttctttacat tttccttttc tagttgttcc aatcttgatc
    12481 gtctgatttt cactaaagca aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca
    12541 aaaataaaat aatgaaattg ataaccatat tctcccgcct ttctcttttt ctattgtacc
    12601 ctttttctac aaaaagattg aaaataatgc gtattcatat ttaggaataa aaaaggagat
    12661 gcggcatttt tcagccacat ctccttatca cttcaaaatc attgcgttac ttgttgctaa
    12721 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
    12781 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
    12841 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
    12901 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
    12961 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
    13021 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
    13081 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
    13141 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
    13201 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
    13261 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
    13321 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
    13381 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
    13441 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
    13501 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
    13561 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
    13621 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
    13681 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
    13741 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
    13801 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
    13861 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
    13921 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
    13981 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
    14041 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
    14101 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
    14161 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
    14221 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
    14281 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
    14341 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
    14401 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
    14461 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
    14521 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
    14581 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
    14641 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
    14701 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agccattgat
    14761 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
    14821 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
    14881 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
    14941 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
    15001 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
    15061 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
    15121 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
    15181 accataaata attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
    15241 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    15301 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    15361 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    15421 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    15481 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaagtaaaa acagctccta
    15541 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    15601 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    15661 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    15721 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    15781 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    15841 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    15901 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    15961 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    16021 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    16081 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    16141 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    16201 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    16261 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    16321 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    16381 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    16441 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    16501 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    16561 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    16621 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    16681 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    16741 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    16801 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    16861 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    16921 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    16981 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    17041 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    17101 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    17161 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    17221 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    17281 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    17341 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    17401 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    17461 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    17521 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    17581 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    17641 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    17701 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    17761 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    17821 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    17881 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    17941 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    18001 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    18061 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    18121 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    18181 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    18241 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    18301 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    18361 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    18421 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    18481 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    18541 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    18601 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    18661 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    18721 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    18781 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    18841 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    18901 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    18961 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    19021 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    19081 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    19141 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    19201 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    19261 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    19321 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    19381 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    19441 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    19501 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    19561 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    19621 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    19681 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    19741 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    19801 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    19861 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    19921 gtttcatcaa atcaacatac gtgtctgtta ttgtttcctt atatccctta tttttcagca
    19981 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    20041 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    20101 ccaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    20161 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    20221 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    20281 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    20341 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    20401 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    20461 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    20521 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    20581 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    20641 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    20701 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    20761 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    20821 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    20881 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    20941 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    21001 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    21061 tttcgacaat gacattgata cagactccca tgaaaattga cgagttgcgg gcttttagcc
    21121 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    21181 tgtgctattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    21241 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    21301 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    21361 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    21421 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    21481 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    21541 gcaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    21601 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    21661 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    21721 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    21781 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    21841 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    21901 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    21961 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    22021 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    22081 ttttcaaaaa ccagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    22141 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    22201 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    22261 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    22321 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    22381 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    22441 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    22501 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    22561 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    22621 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    22681 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    22741 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    22801 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    22861 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    22921 gcaaaaagtt tagcagatct ttctggagca aagatcactg ctcagttaaa tacattccac
    22981 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    23041 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    23101 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    23161 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    23221 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    23281 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    23341 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    23401 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    23461 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    23521 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    23581 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    23641 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    23701 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    23761 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    23821 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    23881 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    23941 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    24001 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    24061 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    24121 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    24181 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    24241 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    24301 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    24361 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    24421 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    24481 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    24541 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    24601 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    24661 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    24721 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    24781 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    24841 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    24901 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga
    24961 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    25021 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    25081 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    25141 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    25201 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    25261 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    25321 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    25381 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    25441 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    25501 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    25561 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    25621 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    25681 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    25741 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    25801 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    25861 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    25921 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    25981 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    26041 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    26101 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    26161 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    26221 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    26281 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    26341 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    26401 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc
    26461 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    26521 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    26581 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    26641 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    26701 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    26761 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    26821 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    26881 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    26941 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    27001 aataactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    27061 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    27121 accatcgttt tatataggga attttctgaa aattgttgca tatttttcga aattttgtta
    27181 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    27241 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    27301 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    27361 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    27421 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    27481 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    27541 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    27601 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    27661 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    27721 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    27781 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    27841 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    27901 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    27961 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    28021 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    28081 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    28141 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    28201 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    28261 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    28321 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    28381 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    28441 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    28501 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    28561 gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa
    28621 tggcttctca catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc
    28681 gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa
    28741 aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca
    28801 ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc
    28861 tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat
    28921 ttcttttata atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DAHEYX010000009        28910 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHEYX010000009 DAHEYX010000000
VERSION     DAHEYX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.009"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            134..376
                     /locus_tag="MB219_000275"
     CDS             134..376
                     /locus_tag="MB219_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCC8426013.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            394..696
                     /locus_tag="MB219_000276"
     CDS             394..696
                     /locus_tag="MB219_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426014.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            767..2821
                     /locus_tag="MB219_000277"
     CDS             767..2821
                     /locus_tag="MB219_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426015.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2821..4653
                     /locus_tag="MB219_000278"
     CDS             2821..4653
                     /locus_tag="MB219_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCC8426016.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            5366..7264
                     /gene="ltrA"
                     /locus_tag="MB219_000279"
     CDS             5366..7264
                     /gene="ltrA"
                     /locus_tag="MB219_000279"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCC8426017.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            7381..8181
                     /locus_tag="MB219_000280"
                     /pseudo
     CDS             7381..8181
                     /locus_tag="MB219_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            8178..10709
                     /locus_tag="MB219_000281"
     CDS             8178..10709
                     /locus_tag="MB219_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426018.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            10759..11091
                     /locus_tag="MB219_000282"
     CDS             10759..11091
                     /locus_tag="MB219_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426019.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11092..11685
                     /locus_tag="MB219_000283"
     CDS             11092..11685
                     /locus_tag="MB219_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426020.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            11848..13872
                     /locus_tag="MB219_000284"
     CDS             11848..13872
                     /locus_tag="MB219_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCC8426021.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            13872..15077
                     /locus_tag="MB219_000285"
     CDS             13872..15077
                     /locus_tag="MB219_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCC8426022.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            15093..15737
                     /locus_tag="MB219_000286"
     CDS             15093..15737
                     /locus_tag="MB219_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426023.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            15748..16554
                     /locus_tag="MB219_000287"
     CDS             15748..16554
                     /locus_tag="MB219_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426024.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            16576..16806
                     /locus_tag="MB219_000288"
     CDS             16576..16806
                     /locus_tag="MB219_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426025.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            17352..17627
                     /locus_tag="MB219_000289"
     CDS             17352..17627
                     /locus_tag="MB219_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426026.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            17669..18091
                     /locus_tag="MB219_000290"
     CDS             17669..18091
                     /locus_tag="MB219_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426027.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            18110..19702
                     /locus_tag="MB219_000291"
     CDS             18110..19702
                     /locus_tag="MB219_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426028.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            19714..20049
                     /locus_tag="MB219_000292"
     CDS             19714..20049
                     /locus_tag="MB219_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426029.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            20196..20390
                     /locus_tag="MB219_000293"
     CDS             20196..20390
                     /locus_tag="MB219_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426030.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            20422..22794
                     /locus_tag="MB219_000294"
     CDS             20422..22794
                     /locus_tag="MB219_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426031.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            22855..24315
                     /locus_tag="MB219_000295"
     CDS             22855..24315
                     /locus_tag="MB219_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCC8426032.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            24326..26530
                     /locus_tag="MB219_000296"
     CDS             24326..26530
                     /locus_tag="MB219_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCC8426033.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            26551..26700
                     /locus_tag="MB219_000297"
     CDS             26551..26700
                     /locus_tag="MB219_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426034.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            26776..27369
                     /locus_tag="MB219_000298"
     CDS             26776..27369
                     /locus_tag="MB219_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCC8426035.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            27385..28284
                     /locus_tag="MB219_000299"
     CDS             27385..28284
                     /locus_tag="MB219_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCC8426036.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            28287..28772
                     /gene="ssb"
                     /locus_tag="MB219_000300"
     CDS             28287..28772
                     /gene="ssb"
                     /locus_tag="MB219_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCC8426037.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT        10883 a         4638 c         5954 g         7435 t
ORIGIN      
        1 ttgttcccta tgagaaggat ggcgtaaaaa aaataatttt tcttggatct agcaaggatg
       61 agaagcgaac gaaagagaaa ttaattcatg atggatcaca atcaaataaa tacaaattac
      121 aaaagactag cgagtgattg ctagtctttt tatttgtcct gaaggaggaa tcacagtggc
      181 aaaagctaaa acgccatttg aaaaagccgc gtctaaaatc ttagaacgcc gcggtgtatc
      241 tgaaacacag tggaaacaag aagtccttga aaaagcacaa tttgacctat tacggggaaa
      301 agacaaggac ttagaaggat atatctatca acgtgaacgt gaaaagcttg ttttcgatga
      361 aattattaaa cactaaaagg agcgaacaat tttatgtttg aatcaattac aatgaatttt
      421 ttaacacaac cgaaagtagt agctgcggtt gatattttta gagcattaca aactgccgca
      481 actagtttga aaaataatct gatgattttt gctgctgttg cggcagtggc ttccattgct
      541 cttggaggta ttttcttctt ctttgggcga agaggagctg aaaacgggaa gacacacgta
      601 ggttatgtat tgttgggggt aggagttatt ttagctgccg cttctattgt agtttcaatg
      661 ttctcaatgt ttggacaaac cccacaagga ctataaaaaa acaagtagaa ggagtaaaaa
      721 gtacgtggct actttttact ccttttttta tggaaggaga atattcatgt tttttacgat
      781 ttcaggcaat gcaaaattgg ataaagagtt aataagaaaa gcaacgaaga aagtgggaaa
      841 gcgtacttcc tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct
      901 acatgtgtta ggacaagtgt tagaacaaga gaatcaatct tctgttaaat tgactctcac
      961 gttacatgat aaagaaggaa aaatagaaaa ggctggcaca gaatacgttt ttggctcact
     1021 ggattatcaa atttcagaag gtttttatcc tacattgatt gatttaatcc gcctagatat
     1081 atttaaagat acggatgaaa ccatgagtgt ggaagagtct tatcgaaacg aaaaaatttt
     1141 acaagtttta gaagaagagt tagaaagcga ccaaagaaaa gtgaatcaag tgagtgaagt
     1201 cgaagcaaac gaagaagtac catgggaacg cccaatcttt ccaaaggatg aatcaaaaga
     1261 aaaagagaaa ggaattattg acgaacaagc tgacattttg actcataaat caatatttga
     1321 ggaagagaac gaggtaatgg gtcaattgga ggtagatccc tttgaagaaa caatggagga
     1381 tgaagagacg gccgaagtag aagttgatcc agttgaagct aatgaagacc caacatggac
     1441 tgaaggggat tctcaggaat atattgaacc acttatcagt gaagaagtag agaaaaatga
     1501 aacagagata caaaaggcaa cggtatttga ttatattttg gatcaatatc cgccagagca
     1561 tgaatggatg aaagaaaaaa ataaattatt tttaaatgaa ctttattcga aatatcaatt
     1621 acctcaaacc tatcaagcat ttttagagag taaagaagaa ttaatctcac agggtaaagg
     1681 cacactttta gaacagttag agtctgtcca atcaacaaat tggcatgagc ttgcacaaga
     1741 agatttatta gctacttttg aagaacgtca acaagaaaca gatcaagaaa ttaatgagta
     1801 tgcgacggaa cagtcgtcga attgggagaa agcaaaagag gaactaaata aagaagaaca
     1861 gcgagtgatt gaagaagaag tacaaaaaat taaagagcgg tacgaaaaga aacgtgacaa
     1921 tgcgtattcc ttgtgtgtag aaaagatcaa caattttact cagacgaatc aagaagcatt
     1981 atcgaaagaa aaagaaaact tattaaaaga acgtataaaa gaacgaaaag aagaatttta
     2041 ccgagggttg cgtactgata agctaaggat tagcaaagaa ttaaatgaac aactgaataa
     2101 actctatcaa actacgactg aagttctgat ggaaaaacac gagcagatcc aacaagaatt
     2161 acaagcacaa attccaaaat ggaaaaaaga gtatgaaatt caccaacaag aattgaaaga
     2221 acaagaagaa aaacaacgga aaaaagaaaa agagcgcgaa caattagaac taaaaaaacg
     2281 tgaacaggat cttgcggaac aaaaaatggc gatagaaaaa caacggctag aacaagaaaa
     2341 ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa atggatacat tgcgacaagc
     2401 acagttaaat tatctgacac atcctcaacc agtagctgct gttcaaacgc cagtgaacaa
     2461 ccaagcaaat ttaactcctt caccccagcg ttcctttaac ggatggatgg tgggatgtat
     2521 tgcaagttta agcttgcttc taggtggagg aacagtattt gcttttaatc atatcaatca
     2581 atcttcacag gctgattctg tagccacaac ggcgtcaatc aaggaagaag cccaggaaca
     2641 agcaagaaaa gagtatgaac aacaattaga agaattattg aatcgtacca atcagtcaac
     2701 aacagagtca agcatggaaa atagcaaaga gagtgattct agtaaggcta aagaaggatc
     2761 aagcgattca actgtacaat caaacacttc cgaaagttca gaatcaaatg aggtgaaata
     2821 atgcgtgtta gagaaacaac gaagcgaaaa gacaaacgat tcaatttaga aaagaatagg
     2881 tatctttctc atttctatcg ctatactccc tcaggagaag agctaccaga aaaacatgac
     2941 aaacgcattt cgaaaaagat tttttatagt ggacttgtat acctcccttt agagtattta
     3001 gtaacaagag taccagaagt tttcaaccga ttggaaattg ttaagggaag cttacaagga
     3061 ataaatctgt ttcaattaca ggaaatgcca caggtctttc attttgattc agctggtttt
     3121 tggaatccat ttactcctac aactagtttg ggtacacgtt tagcaatttc gacagtttgt
     3181 gtggttccag ctgtacttat gggattgaaa atggacatgt ggtttcgtcc tctagcagat
     3241 ggacaaaaag gggataatcg attaatgacc ttaaaagaaa tccaacagat ttatccgcaa
     3301 atacctgaaa caaaaactac ttttccaggt tatggaggta ttccaatcac acattacaaa
     3361 aaatattggt acattcaaac agatactgtt aatacgtgta ttgttggtac ctctcgatca
     3421 gggaaaggtc agattgaagt gttagccacc attgacaatt tatctcgtgc agaaaaacaa
     3481 agttcaatgg tcgttaatga tccaaaaaca gaattatatg ttggtagtaa agatgaacta
     3541 gaagctagag ggtatgatgt atatgccttt aatatcaccg atccactgca atcaatgagt
     3601 gataatccat tggcactcat cgtgaaatac tggaaacgag gagacataga tactgcaaca
     3661 cagttaacca atactttcac aaatacgatt tattatgatg ccaacgcttc agataacaaa
     3721 tactttaacg aaaacgcgca gaaagctgtc aatgcgctta tttttgcgtt gttagaacaa
     3781 gctgatcaaa caggagatta ctccaaattg acaccgaata atctcgttga gcttctttct
     3841 gagattggcg gttttaatta ttatccagat ccagacaatg aattcaaaca gttgaatgcc
     3901 ttagatgaat ttatgaatca gttgccacct ggaaacattg caaaaaaaca atatggggca
     3961 acgaaaattg gatctgataa agcgaaggga aatattttat caacggcaat cacgggattg
     4021 aatccattta ctctaacgaa aattgcaaag atgacttctc aatcaacgat tgattataaa
     4081 tcggttgggt ttccaaaata cttagatttg aaattacatg aaagtctttt aaatcaacga
     4141 gtcgtagtag aattttatca taagggaaag aaatttcatg aagaacgtgt caaagtaggc
     4201 tataaaggat ttagtgaaat caattttgat tgtcatttag ttgcaggtga tcaagtttgt
     4261 ttaaaatacg atcatcaagg aaaagaatat gtggcttggt atgaattttc tcaaagagta
     4321 ttgaaagacg aagagggtaa tatcgtttat cggaaaaaac gaggagaaac ccatttgcca
     4381 gaattagaaa aagaagccgt tctaacatta gatgaggaaa aaagtaactt attagtagag
     4441 tctattcgga tgcactatac cgataaacca atggctattt ttatgcttac acctgattat
     4501 gatccatcta accatatcat tgttagtatt tttcttagtc agttatataa agaattatct
     4561 acgcaatgtg ttaagactaa aggagataaa tgtcaccgca ggattcattt tattttagat
     4621 gaatttggga atagtgtgac ctgtttagcc taaggataaa agaggctagt tccaacgaac
     4681 attggaataa ctttactatc gagaaatcaa atgacggggt aacgccctga agggtttcca
     4741 ctgatactgc gacatgcgtt agtaggatgt gcatccaaac gcgtatgaag ctcgctgaag
     4801 tcggcagaag ggtaccgtaa ggaaagacac gatacgtatc taccacgcaa gtgctctaga
     4861 ggtagggtat gtacctgata tgtcgggtgt cctaaaaagt tcaataaggt gagaatgatt
     4921 tgattatctg acaaacttgc gaatggaatg tctgaaacga ataactcata gaaatgtgag
     4981 ggggaagggg gatacgtcct ctttccattg actcgaggtt gagtaaaaat gtgtttctat
     5041 gaaaaacctt gcatggttaa aggctatgga gtagtcaaca ggcaaaagca agcacctaag
     5101 ttgaaatcta tggatagata gtaaggaaca ggggaagcat ctagcacgga gggagcatac
     5161 cgaagatgtg tttctaagga aaaaccgaaa tcggttataa cttaggtgaa tagatgtgaa
     5221 agtagagcca cgaccagtga aaatttcgta atgaaattgg aggaacaggc tcaagtcgtt
     5281 atttataata taagaaacct atttaaaatc atggattctc gtaagactaa aaaggtcacg
     5341 aaccagaatg gagcgtgata aaaccatgac taacacaaaa ggaattagct taaagaacaa
     5401 acaactcaga cacgccgaat actacgacat gactgagact ttcgatgaat tgcacgcaat
     5461 gagtgttgca aaatcaaatt ttaaaaatct catgaaattc attattgatg acaataatat
     5521 ctttctagcg tatagaaata tcaaaaagaa taaaggaagt gcaacggccg gatacgatgg
     5581 tgtcactata aaagacattg aaaaattggc atgtggtgac tttttaacta ttattaaaag
     5641 gcgatttaag gattacagac caagaaaagt taaaagagta gagatcccga aaccaaacgg
     5701 aaaacttcga cctttaggaa ttccttcaat atgggacagg atagtccagc aatgtattct
     5761 acaaatttta gaaccaatat gcgaagcaaa atttaataag catagctatg gattcagacc
     5821 aaattgttct acagaaaacg ccatagcaga ttgtcttgct agaatcaatc aaagtaagat
     5881 gaattatgtt gtagatattg atataaaagg attctttgac gaagtaaacc ataccaaact
     5941 tatgcgtcaa atatggacaa tggggattcg agataaacaa ctccttgtaa ttattcgtaa
     6001 gatgttaaag gctccgattg ttatgcgaaa tggcaatgtt gtttttccaa ccaagggtac
     6061 tccacaagga gggattttaa gtccactgct tgcgaatata tgcctgaatg aatttgattg
     6121 gtggatagct aatcaatggg aggaacgaac tgtcaaagag ctgaaacacc actatcgaaa
     6181 aaacggagca tgggataaag gaaatatcta ttcgaaatta agaagccaca caagtttaaa
     6241 agagatgtat ctggtacgct atgcagatga ttttaaaatt ttctgccgaa caggagaaga
     6301 agcccaaagg gctttttatg ccacacaaaa atggctcaga gaaagattaa agctcccaat
     6361 ttcaatggag aaatctaaag ttacgaacct aaataaagct gaaagtgaat tcttgggttt
     6421 ttcaataaga ctccacaaaa agggaaaaaa gaaagtggct ttcacgtacg tttgtcgcaa
     6481 agcagaagaa aacattaaaa ataaactaaa acaacagatt aaaaaaattc aatacgctgg
     6541 agcaaaaaaa aataggcacc gagcaatagg tgactacaac atgatggtca tcggtatcca
     6601 caattactat cggatagctt cacattgtag tgtcaatttc actgagattt atagaagtgt
     6661 taggacagta atagaagcaa gactgaaagc tagaggaatg acgaaaaatg gaatctatgg
     6721 acaagatcat gaaggatacc gtccgtatct aaagtctaag caaatgagat atattgaagg
     6781 ttttccgata cttccaattg gctatattaa aaaaagaact ccactcaaca agcggagaaa
     6841 tctaaataaa tataccaagg aagggagaaa gctcattcat caagagcaac aagctataga
     6901 aagctggaaa atcaagtatc ttgtaaaagg ttttagtaac aataagaaat acacggtgga
     6961 attttctgat aacaaaatat ctaagtttat ttcccaaaaa gggaaatgcg ctatcacagg
     7021 aaacgaactt ttgatggaag aaattgaatg ccatcacatt aacccatatc atttgtccaa
     7081 agacgatagt tacaagaacc ttgtcattat ctctagagaa atccatagat taatccatgc
     7141 aactagtgaa gaaactattc tgaaactgct tcggttactg aaactaaatc actatcaaat
     7201 tagaaaacta aacacgtatc gaaaaaaagc agctaatttt ccaatatcaa tcaaaatgaa
     7261 ttagatagga aacaaattat attgtaatta acgatggaac gccgtgtgcg gtgaaagtcg
     7321 cacgcacggt gtggaccggg ggaaaagacg gagataatat caaactctta cctatcggaa
     7381 ttgccaccaa tggataatat ggaaggaatc atgacggtca cagctggccg aaacatgttg
     7441 tgggatctgt ttattcaatc ttatcagcaa ctttatgcga agtatggaga acaaaacgca
     7501 gcgattatca aagaaaattg tcagacacat atttatatta tgtcgacaga tgataatacc
     7561 atcgaagaat tttctaaaaa agtggggaat aagacggttg aacaagagaa cagtaccatc
     7621 aattcattag gtatgaatac tcatatcaat cgcaatgttg attcagatcg tatcttaact
     7681 cctgaaagaa tttctacttt tttagaaggt gaaacgattg tactcgctcc tttaaaacga
     7741 caagatttga aaggtcataa aatacggccg tatcctattt ttaatcatgg ggaaacgaat
     7801 cttccttatg cttatcaata tattgaagat ttcaatccat cgtgggatat caatgatata
     7861 gacatacatt gtacacatgc ggacttggat cttaaggctt tacaaattga ttatgattat
     7921 ttcctaccag atgaatatgc tagaaatctc tatcatggac tgcatgatca attagaagaa
     7981 aaaaacgaag aaaatatgtt ttataaaatc gttcaatctc ttgagaatga gacggctaaa
     8041 aaattgttaa ttcaatttta cgaagaacaa gcagcagatc gacttcaaga atatgttgaa
     8101 aagttgatca aaaatgaaga aatttctgaa atgattggga aacggttgtt attggaatct
     8161 gaaaaggtgg tgaaagaatg atcattggag gaattttttc tggcatagcc gactggtggt
     8221 caggcttatt cggagaaggg gatgcaaacc ccaaaaccgt aacgaaagtc ttacaagatt
     8281 atggtaatta tctgcagtat caagatgtgt tcagttggat ttgtaatact tttttatggg
     8341 ggttgatcaa agggttatat aatctcaata ctttactaga aaaaaccatt tatcaaagct
     8401 ttgatttaaa agacttattg aacgccgctg gggtaaatga actatttcaa agtttgatta
     8461 gtaaggtatt ggctcttttt tgtgtcgtca cattaattta tttaggcttg aaatttttta
     8521 tgagtaaaca cccaccaaaa gtcaaaaata tcttggttaa tttatttatg gccatgatct
     8581 tgatttttgg tggtagttct ttaatcgatg aagggttaaa tatcagtaaa agcttttacg
     8641 gagacgtcac tgcagcaaat aaagcagata aaacgggttc acctgctttt caaattattc
     8701 aaaataatgt gatcgatgtc agtacattac tagcaactga cccgaataaa gtagaatccc
     8761 tgccaacaga caaaagaaat gctttaacag cgaaaaattt taaaacagcg aatatcaatg
     8821 ggattattac tcctaaacaa gctaaagaaa tggcggataa agataagagt ggtttatcag
     8881 atttacaaaa ggatcggtta agagcgttac aataccgact ggaactggat gataatggaa
     8941 aagaaattcc tgtgcaaatc agtgatgaag gcttagccaa atatgtttat acttcagggt
     9001 atcgtcgata cattagtagt ccagggatta ttttagctgg tgaatcttct ttggcggtgg
     9061 cctatttatt tatcctattt acgatcgtta cttgcattat tgagctggta tttaaaaaat
     9121 tctatttagt gattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcta
     9181 tcgaagatat ttctcaatcc ttcttgttat tggcatttac aattttagaa ttacgaatct
     9241 ataccttaat gctttcaggt attggtgatt tgcatgcggc aggaaagatc aatggattct
     9301 tgtatgttgt cgctttgatt gtactaacca ttgcgctatt caaaggctct caatcagtta
     9361 cgaaaatttt cggcgttgat acgagtctga aaaacggcag taattctctt cttagcatgt
     9421 ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc taaaaacctt gcaagtgctg
     9481 gtaagagtgg tttaagccat ttaaatgata ttcgtaaaaa tggatttggc catcgaaaag
     9541 atgttacccc tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata
     9601 gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag
     9661 aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa
     9721 aagccaaaga caccttagaa gataaattag acggcacgag agctaaagaa ttggctgaag
     9781 ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gaagtcaaag
     9841 aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtgaaa gctaatacaa
     9901 caaccgaaaa agaaaatcaa gaaggtatag tgattccttc agtcaaagat caccaagaag
     9961 cgttgcactc taatgaaaat tcgattgaag agaaaaattc catagaagaa agaaattcag
    10021 aagaaggaaa aaatggacag ttaccaaaaa ctgatccaga acaatatcgc tataaaccta
    10081 acagtgacct tgaaaatgag aagcgaattt cagatttatc aaaagtaaat caagaagaca
    10141 aagcagaaaa tgagggacag ttaccaaaaa ctgatccaga acaatatcgg tatagatctg
    10201 ttaactccga aaatgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa
    10261 cggatgagga ttccatcgtt ctgccaaatg ataatggaat cgaagttgga actgaaaaat
    10321 caacgaaaat tggtttacct aaggcacaaa atttaccaat gaaagatagt cgtttgaatc
    10381 aattcaagga taatgatcaa agttttgttc atcaatcacc tgcaggtact gttcaaatga
    10441 aaccaagtcg tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttatctgaaa
    10501 gtggtgagac agggaatgcg tccgttcaaa atgttgtgac aagagaagct gcaccaaaac
    10561 ctattcaaaa cgttcataca ggttacaagc cagcaaatgt ggcatttact gcaccaacaa
    10621 aaataaatgt gacaagagaa actaaaaaca ggaacaatgc attgaaagaa caactattga
    10681 gggatatcaa acaggatatc aaaccataaa aggcctacaa gttaggcctt ttctttatat
    10741 gaaaaggaga aatcaattat ggataaatat ataaacatga aaaaccctcc aagatttagt
    10801 ggagaatgga agtttttcgc ttttttcttt ttagatctat taacgcttgg tggctgcttt
    10861 ttttgggcga aaatggtcaa taatcttgtc cacttaccac tcttttttgc actcttgaat
    10921 tatggcttat ggattgcgat agcgttagtg attgtttggc gaccctataa taacccaggt
    10981 caacgacaaa tttttgcgat tatcgagtct attttttttg cggacaagaa ccattatcat
    11041 gcgttagatc ctaaccgaaa ttatcatggt acgtataagg aggatgaata aatgcgagtc
    11101 aaaggacaaa attcagttga aaggaaatcc attttgcagt ttggaaaacc agaagagcgc
    11161 tctatcttgg acttatttcc gtataaatcc tgtacagaag agggagtctt aattactaga
    11221 gaagatcatt ttcaacgtta ttatcgtgta gtttctacag acgtggaagg attaaatgaa
    11281 caagagaaac tagaacgaat gaatcagttg accaccgtca tgcgtacgta tgtgccaaca
    11341 attaagttag ttagtttgac cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg
    11401 cagttattag ataaaaatcg tatggaacag tccttaggaa gaaatctaca gcgattaaga
    11461 aaatatgaaa agaaattagt agaggaaatc caggaattga aacgagcaga acaggaacgc
    11521 cctgatttaa gcttcttttt cgtcattgaa ggaaagacaa tcaaggattt gcaaaccaga
    11581 aaccgtcagt taatgcgatc tagtggcatt ttagggttaa aaccactcta caaaaaagaa
    11641 ttgatcaaga ttctttatcg aatgaataat atgaatgatg agtaggaatg gggaagagag
    11701 aatgaaatat ccgttgatat ggttagaaaa agatgttccg aaagaagaga ccactagaaa
    11761 atttcctcac actttaagcc tgggtaagtt gggaaaagga aaatctttta accaagtaaa
    11821 gaatcaaaca agaagaggtg actgtacatg ttagaagcaa ttaaaacagc tttttcacca
    11881 caaaatgatc aaaaggtact tgatccatta aagcaactag gaaatcggga aatagaaatt
    11941 ttaaaatcca aagggtatga tttagacttt ttaaaacaag tgatgccaca aggtggatta
    12001 aatttcgatg aagagtatgt gacctatgga aatggttatt cacgatgtgt gacagtttat
    12061 cactacacaa aaaatcctac gcttttttgg ttggccaatc tcatgaacaa tcctcataca
    12121 gtggcaacac tagatgttag cacagatgaa aagcaaaaag tgattgatca aatcaatcgt
    12181 gccttagatg aattaaatga tcggtcagat aatgaacgaa aagcgactga ccgaaataaa
    12241 agcgcggaag aatacctaga actagcggaa tatgcccgaa aattgacttc taatggggaa
    12301 atttctaaac aagtcaaagt gagagtacat gtttacggaa acacgattca agaagtagat
    12361 gaacgagcac aggaactgat cgaaaatctc aaagggatgg attatcgtgc agtgacctat
    12421 ctgcacaaaa cgaaaagaga ctggcaatcg atgttcacca tgttcgatga acaagctgaa
    12481 tggtttggaa atatcaaagg gcaatcacta ccatcgctaa acattggcgg aggtttcccg
    12541 ttccaccacc aaacgctaaa agatccaggt ggaacacaat atggggaaac catgacaggc
    12601 ggcccgtttg tttggaatgc cacgtataaa aatagtgtgc ggttgtctac caataccttg
    12661 cttctaggaa tgatgggagc tggtaaatcg accgttctta aaatgatcgc ggaagcccat
    12721 ttagctgcag gagactttat ttggggcttt gaaaagggaa aagactttat tcccttttta
    12781 aaggaatata acggcattat ggtgcgttta gacggatctg acgggatgat caatccttta
    12841 gaaatatttg ccacacgtac gtatgatgaa gcttccagtt tatatgacga tggatcagtc
    12901 aaagatttga agatcaatga agcagcgtcc tatcaaacgc acttagacaa agtggtctat
    12961 caggtacagt tagtgagtcc tcagctaaaa ggaacgatga aagcagaatt taagacctat
    13021 ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg gctttacttc ctcaaattcg
    13081 cgttcaaata ctgatacgca agtcacaggt aaagatccag aagcttatcc gacgttcaaa
    13141 gaatttttag actttttagg acaactggaa ttaccaggtg cttcccaaga gaaaaagaat
    13201 cgaaaagaag aaatggaatc catcgtagaa tccttatgtg agacgtatgg catgattttt
    13261 gatggtcata gtacgattcg acaccttgac caacaacagt tggtcttttt tgacattgac
    13321 tctattacag gcttagatcg aggggtacaa caatgtcaaa tgtacatggc tatgacgttg
    13381 atttggaatc aggcattgat tcaaggaaga agacaacgac gtttaattaa agaaaaacaa
    13441 atggaagaac aagacttcaa atgcttcttg gcgatgattg atgagtgcca taatgcgatt
    13501 aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa
    13561 gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg
    13621 tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt
    13681 atgcgtatgg atgaatctat tttacgtcat atagaaggat tagttggtaa atcaatgaca
    13741 aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
    13801 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat
    13861 ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg
    13921 caaagcgacg cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga
    13981 ttactgtgtt cgtgaccctt tttctaatac ttgcggcggg ttcgattgat aattctgatt
    14041 cagattcaag tagtggtggc gaagctttta caggagaata ttcggaaggc ttaccgattt
    14101 ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gctgtaggtg
    14161 cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt tatgaatcag
    14221 cgtttggtac gtcggcgtcc gcaagaaatg atctcaacta ttttgggatt acttggtttg
    14281 atggttgtct gtttcctaaa ggaactgcgc gaggaatcgg tggtattgaa ggtggctggt
    14341 acatgaagtt tcccaactca aaagcagcct tctcctatta cggctttatg gtagccacac
    14401 aatccaattt taatgcatgt gtaggaaata aaagccctgg cgcaagctta ttgatcttag
    14461 gacgaggtgg ttatgccgct gcaggaatta ctgaagatag tccgtattat acaggttgta
    14521 tgtccattat cacttctaat aaattaaccg aatatgatga attcgccatc aaacattggg
    14581 gtgaaggtgg caataacaac ggcacgatca ctggtgaatg gacgaatcca ttccctggca
    14641 gcagtttaga taaaagttcc ttcagtggtg gtcaattatt tggaacaaat ccaggcggtg
    14701 aattccgtcc gaatggcttc catgatggat tagactttgg ttcagtcgat catccaggaa
    14761 gtgaaattca cgcggtgcat ggaggaaaag tcgtgtacgt tggcaatcct ggtatttcag
    14821 gtttaggtgc atgtgtgatc gtgattaatt acgacggttt gaatatggta tatcaagaat
    14881 ttgcgaatag tacaggaaat tctcgtgtga aggttggcga tcaagtgaaa gttggtcaag
    14941 taatcgggat acgagataca gcacacttgc atttaggttt cactcgaatg gattggcgtc
    15001 aagcacaagg acacgcattc acagacgatg gtacatggat tgatccatta ccatctttga
    15061 atagcagcaa aaaatagatc aggagaaaaa atatggaaaa taaaacttta aaaatcgtct
    15121 taacagctgc aattagcgtt agtttaatcg gcaatatcgc gttgttttgg cagctgaaag
    15181 aagaacaaga gacaaatacc tttaaaattg aagaagtggc taaaaacgcg tctgagaagc
    15241 tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg
    15301 agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag
    15361 taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt gataaatcat
    15421 taagtagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca
    15481 aattaagtcc tgcagacgat gatcaccaac atgatgatta cggaagttac tcctttacat
    15541 taggtgacgt gcaaacttat gctcaaacaa gcagtgtaaa agaggaacaa aattttgtcg
    15601 tatttgttga ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca caaaaaataa
    15661 aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc atagacttta
    15721 gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag acaacgtttc
    15781 aaaaaacgaa aaagttatct tcaatggctg ggaatcttta tgattctcgg ctttttgatt
    15841 cttgctagtc cgatttatac gaatcagaag gtagatatac cagatgaggt aagcacgaac
    15901 gcctttttaa acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc gaaccagcaa
    15961 ttagtttttg aatttgcgat cgatgatgaa gatcaaagct tgttaaaaga actggctaat
    16021 cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat acgagccaaa
    16081 gtgatcaagg tgtctgatga ttattttgca gtgaagctaa cgcatgtacc agaagaatat
    16141 atcgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga tatgcaagaa
    16201 ccacaagatc tgatttatta cgtgcacgaa gataaggtta aagatcaagt gaaagatgaa
    16261 gattatgaac aacacgcgat caattacaaa gtgaaaggat acaagaaaga acaaaaagca
    16321 gctcaaaaac aaatggaatc tttccaagca accattgatt tgaatgaaga attagtgaag
    16381 aagctaaaag atcaattgcc ttatcaagtc gcagatgatc aggaaaatac tgaaaacaag
    16441 attaatagct accagcaaga aattgaaaca tcgaaaacgc aactgaaaga tcaagaagag
    16501 atcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaagat ttagaaggac
    16561 aggtggagtg aaaaaatgaa gcgaacaaac ttggctgaca aagatacttt cgctgagcag
    16621 aaagtattga ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcgagaagtg
    16681 aatttatcta acacaacaat atctgagttg aagaatggtc gaaagaattt agatacttta
    16741 aaattcagat catttgaagc tctatatgta gctgctaaaa aatattttac agctgataac
    16801 aaataaaaaa caggagtatt ttatgtgaaa aaagtatata aaaaatgctt atgaatcagc
    16861 tatatcacct taaccatttg ttaatgagat gggagaaaac attaaccacg attctaacgc
    16921 aaacatcaag cacataaaac atttaacact cctcctttct ggctgaagat ttcaaccaaa
    16981 caggaagaca gcgtatcaat tatagcatac actttttctt aaattcattt ttcatatgac
    17041 aaaatatttc cttctaatcg acaaagttag aaggattttt tattgtctaa atacacaaat
    17101 aaccttaatt tttaaagagc ataaggaaac gtgatatcac tttctcacga aaggtgttag
    17161 gtaggctgtg cctaccacgt ttctaagaaa gataaatgat atcaaaattt tggatttgtg
    17221 tgtagatttg tgggtaggat gaaagcgaat gtgggcagta aaataagcgt ttgtgtgtag
    17281 caaaatcaaa aaatgtgtag ggatatgtgt agctacacat atccctacac ataataaatt
    17341 tggaggaaaa tatggactta ttaattagaa atattgaaga aaagtatatt aataaaattg
    17401 acaaacgttg tgaagaactt acggtcaaga ctggcaaaaa atggagtcga aatcaatacc
    17461 taaaagtttt gatcgaaaat gattttgatc acgctcttct gaattataaa aaagaccaat
    17521 ttgatcggtt gttagaaaaa tttgtggata ttcaaactta taatacgaaa actttagaag
    17581 aatatattgc aacaaataat caattaattg gtttgttaat tgaataataa aaatagtatt
    17641 gtgaatttat aaaaggagga aatcaatttt ggcaaaagca acaagaaaac atgtatatct
    17701 ttacgatatg catatcgaag tacttgatga aataatttct actcatgaag atatatctaa
    17761 ttatagtgaa gcagtcagac atttgtgtat gatgtatgat ccaaaaatcg aagaaaaaat
    17821 aaatccaatt caaattaaat tgaatgcaat gggaaaagaa atttctattt taaccgaaat
    17881 ggtttcagag tttgcggatc ttcatttgaa agatacagga atactaattg gtacaaattc
    17941 aactgtttat caagaagcag aagaacgtgt ggaaggaaaa attaaatcca atcaaaccaa
    18001 aaaatattct tctaagttaa agaaaccagt gaatgaatct gttcatacca gagtagatcc
    18061 atttgatcca acgaagattc taaaaagata aaagtagaag gagaacgtga tgaataaatc
    18121 gcctgcagtt attcttactt gtcaatttac cttgcccaat aaaaaatctt tttcaacata
    18181 cattgattat atgacaagag aaaaagcact ggaagaaatg gaacgacgta cgcccgaaga
    18241 agaacaggaa ctgcaacgaa tcaagaatgc tttagaggat ttttatattc ctgaaggaga
    18301 aactttttcg gaaagtaaga cggataaaga attcagtgaa acgtcgaatg aagctcaatc
    18361 gttgatggaa catggaatca attttgacaa gttagaggaa caagatttta ccaaatatct
    18421 ttcttatatg acccgtcact atgcgttaga acaaaagaaa gaactgacgc aaagggaaca
    18481 aaaagaatac caacggttga accaagcaat gaaaaaatat caaacagagc cacataaaaa
    18541 agcttcagga aatcttcctg gagttttttc tatggcttca gacgaagtga aaggaagtga
    18601 tctgaaggag atcaaacaaa tcttccaaag aggacaacaa aaaggctcaa tcatttatca
    18661 agatgttatc tctcacgaca attcgtatct tgaaaaaatg ggcttgtacg atcccaaaac
    18721 agatacctta gatgaagaag gtttgaaagc agctggaaga aaaatgatgg agacattatt
    18781 cgagaaagaa caattgaatg aaacaggtta ctggatggca acgattcacc gtaacactaa
    18841 gcatatccat attcattttg cagttgttga aaaagaaaat acgcgtaaac cgtatactga
    18901 gattgaaaat ggtgtgagtt atatcgtacc gaaggggaag agaagacaag caacattaga
    18961 cgcaatgaaa accaattatg tacatgagtt agaacgtttc tcttatgaaa aggatcgaat
    19021 gatcggtatg gagaaacgaa atttattgac acgaaaaagt gatttgcgta acaccttaac
    19081 aaaagtagta aaagaaccag cacgttacga tcgacacgcc atgaacttat taaatgaagt
    19141 atataaaacc ctacctgaaa aacgttcgga atggaattat ggagatgaaa atcgaaccaa
    19201 gctttcacca tttacaaggg gaaaacttga tgagttaacc aaatatgttt taacaaaaga
    19261 acctggatat caagaatata tacagatttc aaaagcattg aaagaagaag ccaaaagcct
    19321 ttatggggaa agcaaaagag aaacaaagaa tgcagaaaaa aacgccctct ttgatttaaa
    19381 aaagcgcaca ggaaatgcga ttttaacaga actgaagaga caggatagtc aaataaaacc
    19441 attaatagaa agtgtcaatc agatgcaatc tatttacaat gaacgatcct ttcgaacact
    19501 caaacagttg ccaacaaatg aatacaagta tagttatcaa gaattcgtaa agcgtaggaa
    19561 acaattccaa caaagagttg tcacgcagcg aagcttgcgt caattaaatc gaacaattaa
    19621 tgcacatgaa gaaaaatacc acgcaagtaa agcatatgaa gagcaacagc gaagaatcca
    19681 aatggaacaa gaaagacaat aggaggaaga gaaatgaaac aatatttaga tcaatggaaa
    19741 gtcatagaag gatcactaaa agaagaacga atcgaacaac ttcctgattg tttagaaaaa
    19801 gagcatttgt ttcagataag cgaaatgttg agaaacgaac agtttgatcc aaatcaattt
    19861 ttagttgttg agtacccagc tactggtgtg tattgttgta atcatgtgaa gggtgaaaaa
    19921 tattttatta ttcaagaata cgaaggtaag ttagcgcctt attatactac ttgggaaata
    19981 aatgaagagg gaatcaataa tttcccatgt gaaagcattg aagagtctat ttctctcacg
    20041 gaatgttaaa taaatagaga cgtaaaagag tcctgaacgt gattgtccag gactcttttt
    20101 caatgaattg ttttccgcca attcactgag tggtgcgatt caattttatt atatcaaaag
    20161 aaagtgagat tccaaagtga agggaggaag aagaaatgac tcagacaacg attggtgttg
    20221 tggttgaaaa aagcagggat aatctcgttt ttgtgacaga aattcaaaca ggtagagctt
    20281 ttgtagtcac agacaaagct gcaaaagcgt atcaaaatgg caatatttta acgttaaatc
    20341 tgactacgaa gatgttcgtt gatgcagtag aaaattatcc atttgtttag acaaacgata
    20401 ttttacaagg aggaaacgga catggcagaa gaaaaagaac cactagataa tagtcgtttg
    20461 ggaaagtcca aaagaaaatt agtacggttg caaaacgaat taaataatca atttcaaaag
    20521 ctatttgatc accaaaaatt gacaaatggg caaccaatga atgacaaacg aaatggtcgt
    20581 tcatggttta accagcaaga aaaaatagaa aataaaatac aaagtttgag agaagagatc
    20641 aagcaacaag aaaaacaggt agaaaaactt gaaagacaag aagagctaaa ggaaatgggt
    20701 tataacaagt acggtggcct ggacatgacg atcgaaaata ttccaagaat caaagaagaa
    20761 atcgaacgct ttgaaaaagg tgaatcgact ttttcagctg ccacgatccg aaaataccaa
    20821 cgaaaattgg aaacattaga acagctgaaa gaaaaatcag agaaaggaaa agaaaatctt
    20881 cttcctgaag tacaagcgat cattgacagc ggacgagtga cgcaatggaa aaagaatcca
    20941 actatctatt ttctgaaagg ctatcgaaaa gtcgctttag aactcaatga aaacggggga
    21001 tttgaggaat ctaaaaaata ccaagccacg tcggaagaag aaaaggcagc tgttcgtgaa
    21061 ttacttgaag aagctacacc tactgattct gtgaaggaga aagaaaccga tcatttctat
    21121 cgagtagaat tcaacgaaaa tgatccagca aaagggttga aaagttatgg tggggagatc
    21181 gtaacactag aattgatcga tcaactcaaa acactggata accaatacgc cgcagaagca
    21241 ggctattata aattctattt tgaagaagtg aagaatggag aagtagtcga tcagatacga
    21301 atggatctag gggatggcgt caatagtaat caccccatct atttagagtt agctagattg
    21361 tgcgaacaat cacctgaaaa ggaacaagaa caaacagtca aaaatgaacc tatttcaaaa
    21421 gtacaaaacg agattaaaga gacaaaaaca aaacaaaagg gttatcaaaa acgaacggca
    21481 gaagagatta agcaagaagt caaatcactt tcccaacagg cgcttgatgc ggtgaaaaag
    21541 catactcatt cacctgaaga cgtcaaagaa cttttgaatt ttatgtctcg ttttccagaa
    21601 cgatccttta gaaatcaatt attgatcgag gaacaatttc caggcgcaac ggcttgttta
    21661 ggtcgtgccg cgttgaaaaa agaaggaatc tatatcaaaa aaggagaaca agggaacaag
    21721 atctttgtca gaaaaacggt caaaggattt tatgagaaag gacgtggctt tgttcgagaa
    21781 acagaggcta caccagaaga taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa
    21841 aaaccttact acacgattga aaaagtgttt gatatcacac aaacacaatt gaaacctgaa
    21901 gactatccga aaatcttccc gaaccgcgtc tttgattttc aattggatca aacagggcag
    21961 acagaacttc aagttgggat tcaagcggta gccaaaaata ttggaattca aattcgagat
    22021 atgacagaaa gtgaagtcta tcaaagagaa ttaggacaag cacgtggggc gtacgttcgt
    22081 aacgaattta ctggcgaaga agaaatcgtc atgaatactc gaaatactcc tacgcagcat
    22141 ttagccacaa gtattcacga attagcccat gctagaatgc acaaattttc agaattagat
    22201 actgcaacaa aagaactaca ggcagaaatg acctcctata ttgtgtgcaa gcattttggg
    22261 atggacacct cggagaaagc tattccttat attgcgacat ggacgaagaa tagtcagacg
    22321 ttggatgata aggaagctgc ggaacgtggg aaaatcatga atgatgtcag tcgtgtcgca
    22381 aatgaattca ttcaaaccat ttctaatgaa atcaatcaat acagagaaag agaaccagag
    22441 atccagccag cagaagtgaa gcaggagcat aaaaatactg tagaaacggc agaacaaaag
    22501 aatattgtgc ccgttggctc actttggatc gacaagaaag acgggaaagt cctggaagaa
    22561 gacacgggcc gaacgttaca tttgaaggat gcggcgttta ttgaagatcc gcagcataat
    22621 caagtgattt taaaaggaaa aagttatgga aaagagatcc aaacggcgtt accttcagaa
    22681 gaatttgcgc taggaaatcc attccgattt tcacttgaga cagtgtcaga agtggcaaaa
    22741 cgaaaaaatg aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag
    22801 caagaaagac tatctgaaaa atggatagtc ttttttcttt tgggaggttt gaaaatgaca
    22861 gagaaaaaaa tgagtctcat tgatcgatgc aagcaaatgg atattgtgga ttttgcacga
    22921 aacaatggtt tagcggtggc caataaagga cgagactatc gcttagaaga ccatgattca
    22981 tttgtttttg atcgaagaaa acaacgtttt tattggaaca gtcaaaatat tagtggcgac
    23041 attattgaat tagctaaact ttttttcata gacaaagaga tccaagaccc taagcaacaa
    23101 tttaaagcag ccactgattt tattctaaag aatgaagaca aaacagaacg agtggaaaat
    23161 cttcattttg agacagaaaa gtacaaagat caccccgttg attatcaacc actcacagaa
    23221 aaaggaagga attatctgaa ggaagaacga aaactgcccg attggttgat tgattatgca
    23281 gaaaaagaag gattgattgc cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt
    23341 cgagacgatc gtttggatca tgcggtcgcc tttttatgga aagatccaca gacaaaagag
    23401 acagtagggg cgagttatca aggaacgttc attgattatg aacgttttgg tgagagaggc
    23461 acatataagc atatagataa aaattcgaca gcgaatcatg gctttaacct aaagattggt
    23521 gatcctaaac agttaaaatt ttttgagagt agtattgatt tacttagcta cgctgcactg
    23581 aatcgtgatc agttaaacga tacgtggtta gtctctatgg aagggttaaa acaccatgtg
    23641 atcagtcatt attttggtga ggcagtatct gaattgagaa agaaacaagc ctttcctcaa
    23701 tccatcgaga tttgtgtgga taatgaccgt gcaggacata ttttctacga aaaagaacaa
    23761 ttaatgggcg cagttgatcc atttacgaat cagaaagtac gttgtgaacg aggaatcgct
    23821 aatgattggc aagtgccaag agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa
    23881 aaggtcacac cagaagctat tatggccata cacaagacag aaaacaatct gcaattaacg
    23941 aaccaactgg tttccgctca caaagtcaat gcgtcttttg gtcagcagct ttctgttaac
    24001 gattcaatag aagtcattag tttgaaagat atttgtagag aagtcgcaaa agaattaaaa
    24061 gattgtgaac gctcgaatgg tacgtatgat tttgatcgtt tttatcaagg aaaaggggat
    24121 attaattctc agattctttt ctcttataaa gcagaacaat actacaaagg ctataaaaat
    24181 catgaacatg aatttgtacc tgaagtgaaa aaggattgga acgatcaatt gaagcatgag
    24241 atccatcaac aggaaattag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
    24301 caagaaaggg agtgagatcg tcacgatgaa aactgtcatt ttagcagaaa aaccgtcaca
    24361 agcgaaggcc tatgccgata gcttttctaa agccacccga aaagacgggt attttgaaat
    24421 ccaagatcga ttgttttcgg gtgaaactgt gatcacgtat ggttttggtc atttagtcga
    24481 gttagattcg cccgatatgt acgatgaaaa ttggaaacaa tggtcattag agcatttgcc
    24541 tattttccct acacattatc actatcatgt tcctaaagat aagaaaaagc aattcaacgt
    24601 ggtgaaacag caacttcaat cggccgacac cattattatt gcgacagata gcgatcgtga
    24661 aggggaactg atcgcttgga cgatcattca acaagcaggc gctgatcaag gaaaaacctt
    24721 taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat tccagcaatt
    24781 acgagacgca gaagagacct atcccaaatt tgaagaagca caagcacgcc agatcgctga
    24841 ttggttgatc ggcatgaacg gcagcccact gtatagttta ctcttgcagc aaaagggcat
    24901 accaggaagt ttttccttag gcagagtcca aaccccaacg ctttacatga tttatcagct
    24961 gcaggaaaag attagaaact tccaaaaaga gccttatttt gaagggaaag cccaagtaac
    25021 cgctcaaaat ggcatatttg atgcgaagct tgatcccaat gaaacccaag cgacgcaaga
    25081 agcatttgaa gcctatttaa aagaaaaagg tgttcaagta ggaaagcagc caggcacgat
    25141 tcttgaagtc gaaaccgaga aaaaaagcgc ggcaagtcca cgtctttttt ctctatccag
    25201 ccttcagtct aaaatgaatc agctcatgaa agctagtgcc aaagatactt tggaagccat
    25261 gcaagggttg tatgaaggga aatatctaag ctatccacga acagacaccc cttatattac
    25321 agaaggagag catgcgtatc tgttggatca tttagatgaa tacaagcatt ttttaaaggc
    25381 tgaatcattc cctactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt
    25441 ccaggaacat tacgcgatca ttcctacaaa aacagttatg acagaagcaa cgtatgaaaa
    25501 gctgacacct ttacaacaag cgatttatga tcaagtttta aagacgacgg tggccatgtt
    25561 tgcggaaaaa tatacctatg aagaaactac gattttgaca caagtccagc agcttcagct
    25621 aaaggcagtc ggaaaagtac cagtagatct aggttggaaa aagctatttg gcaaagagag
    25681 cgacagaaaa gagaaggaag aagaaccgct acttcctaaa gtaacaaaag gagaaacggt
    25741 gacggtagat ctacaaatct ttgaaaaaga aaccaagcca ccacaacctt acacggaagg
    25801 tacactaatc accgccatga agacagctgg taaaaccgtg gatagtgaag aagcacaatc
    25861 catattgaaa gaagtggagg gaattggtac agaagcgaca cgagcaaata ttattgaaac
    25921 cttgaagcaa aaggaatata tcaaggtaga gaaaaataaa cttgtggtga cgaataaagg
    25981 aattttgctt tgtcaggctg ttgaaaaaga accgctgtta acaagtgccg aaatgaccgc
    26041 caaatgggaa agctatctta caaagattgg tgagcaaaaa gggacacaag ctacttttct
    26101 agcaaatatc caaaaatttg tttcccactt gttggaagtc gtcccaggac aaatccaagc
    26161 cactgatttt ggagcaacgt tacaagaggt gaaagtagcc agtgaaaagc aagaagcggc
    26221 gcgtcattta ggatcttgtc caaagtgcca ggaaaaagaa gtcatacttt atcagaaggt
    26281 ggcgacttgc acgtctgaag cctgtgattt taaattgtgg gtaacgattg ccaaaaagaa
    26341 actcacagcg acacaactga aagagatcat tcagaacggc cgaacaagtc aaccagtcag
    26401 aggactaaaa ggtcagaaag gttcgtttga agcaacgatc gttttaaaag aagactttac
    26461 aacaggattt gagttttctg aaaagaagaa gaccacctac aaaagaaaag tgagacgtaa
    26521 aacgagttag aattgacgga gggaaaaacg atgagaaata caacgcataa agaacaagaa
    26581 atcgaaaatc aaaaagccaa aatacgttca gagcaggaga aaatgcgtgc cagcaaacga
    26641 agactacagc aattgaagaa tcaacaacgg attcaaaaag aacttgaatt agatcgataa
    26701 atcctcctgt tgttaatatt aaactaacat gttatagtta tgttagttta atattaacat
    26761 tcgagaggga aagcgatgaa agataaagtc aagtgcattt ttgagaaata caatggtaat
    26821 ctatccttga aggatgctag aaatgaaggg ttatcaccta tgacgttaga tcgtttagcc
    26881 gaaaaggaca aaatagaaaa aatagcatca ggtttttata ccttaaaaga tagttttgtg
    26941 gatgaattat atctcgctca aagtatctat actagaggaa tcttctctca cgagacagcg
    27001 cttgatcttt ttcaagtagg aacatatatc cctaaaaaaa ttaatatgat gtttcctaaa
    27061 ggatacaatg tttcgaaaga gaatttagat cagtatgcca ttcaaccgca ttacacgaat
    27121 aaagaaaact ttgaactggg aatcacagag gcagaaagct tttacggcaa taaaattttc
    27181 gtctatgatt tagagcgaac gttatgtgat atgtggaatc ccagatataa agcaagcgta
    27241 gaagtaaaac aagaagcatt aaaagaatat atgacaagta ccagcagaaa tacaaataag
    27301 ctaagaagat acatgaatat tttaaaatca ccaaaagaaa tgactttgta tatgttgcca
    27361 ctttattgag aggagtaaag gtgaatgaca ccgacacaat ttaaagcgaa aacaagaaat
    27421 gttgcagctg agaaacattt aaacccacaa attgttcaaa gatattatat gatggaaaaa
    27481 ttattagaat gtattgttga atcgccttat catgatgatt ttgtattaaa agggggattt
    27541 ctgatcggat caaaatacgg gttagaaaac cgttcaaccg ttgatattga tacgacttat
    27601 cgaaattcga aactaacaaa agaaaaatta gaaactgttt tttctgaatt aacagctgct
    27661 cctacaaaag atggcattcg gtttgaatta aagggcttaa cagagacgag ggaagccgat
    27721 tattacccag gttttcaagc aaagtttatt gcttttcttg aaaatgcacg tgtacctttt
    27781 aaattagata ttacttcagg tgattctatt attccagcag cattgatata tgaacataaa
    27841 ttgatgtttg aagataaaac aattcatatt ccagcttatc ctacggaaca aattttagct
    27901 gaaaaattac atgccacgtt tagtttcgga atagataatt cacgtatgaa agatttctat
    27961 gatctgtata tgatccctaa actagaacgt atcgatgaga taaatttgta taagagtgtt
    28021 caaggaacat ttaaggaaag aaataatcaa tcagtgttta agaatttgta taatcaggaa
    28081 atgcctcaat tgatgaagaa tgagggattg agggagaaat ggaaaagata tcagcaagaa
    28141 aactattttg ccaaagatat ttctttcgaa tctaccttaa cttctgttga ttcgctgatg
    28201 caatcaattg tcgagcaaga ggaagtagaa cgaaaacaaa attttaagag atcacaacaa
    28261 atgaatcaag aaatggagag ataaagatga tcaataatgt aactttagta ggtcgattaa
    28321 ccaaagatcc cgatttacgt tacaccgcaa gtggcactgc agtgaccact tttaccttag
    28381 ccgttaaccg taattttact aatcaaagtg gcaatcgaga agctgacttt atcaattgtg
    28441 tgatttggcg caaaccagct gaaacgatag cgacattagc taaaaaaggc agcttgatcg
    28501 gcgtcgtcgg acggattcaa acgcggactt atgacaacca gcaaggacag cgtgtctatg
    28561 tgactgaagt tgtggccgat aatttccaac tattagaaag taaagcagcg acagaaagcc
    28621 gtatgcacgc tgatcaatcg tccagcagtc caagccctac cacctttgaa caacgggata
    28681 cagcgacgcc taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa
    28741 tcgatattag cgatgatgat ctaccgttct aagtagcatt ttctattggt tgcatttgct
    28801 acggtttatg ttataattta gacaacaaaa agaattttca atctataacc aaaaaccact
    28861 agctggcggg ctagtggctt tcttttacgt ttctattata agaattaacg
//
LOCUS       DAHEYX010000010        27989 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHEYX010000010 DAHEYX010000000
VERSION     DAHEYX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.010"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(50..277)
                     /locus_tag="MB219_000301"
     CDS             complement(50..277)
                     /locus_tag="MB219_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426038.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(444..2414)
                     /locus_tag="MB219_000302"
     CDS             complement(444..2414)
                     /locus_tag="MB219_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426039.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2429..3295)
                     /locus_tag="MB219_000303"
     CDS             complement(2429..3295)
                     /locus_tag="MB219_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426040.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3285..3518)
                     /locus_tag="MB219_000304"
     CDS             complement(3285..3518)
                     /locus_tag="MB219_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCC8426041.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3530..5686)
                     /locus_tag="MB219_000305"
     CDS             complement(3530..5686)
                     /locus_tag="MB219_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426042.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5706..5927)
                     /locus_tag="MB219_000306"
     CDS             complement(5706..5927)
                     /locus_tag="MB219_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426043.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6037..6375)
                     /locus_tag="MB219_000307"
     CDS             complement(6037..6375)
                     /locus_tag="MB219_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426044.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6775..10416)
                     /locus_tag="MB219_000308"
     CDS             complement(6775..10416)
                     /locus_tag="MB219_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426045.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10419..12368)
                     /locus_tag="MB219_000309"
     CDS             complement(10419..12368)
                     /locus_tag="MB219_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCC8426046.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12410..13468)
                     /locus_tag="MB219_000310"
     CDS             complement(12410..13468)
                     /locus_tag="MB219_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCC8426047.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13587..14042)
                     /locus_tag="MB219_000311"
     CDS             complement(13587..14042)
                     /locus_tag="MB219_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426048.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14626..15429)
                     /locus_tag="MB219_000312"
     CDS             complement(14626..15429)
                     /locus_tag="MB219_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCC8426049.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16410..17633
                     /locus_tag="MB219_000313"
     CDS             16410..17633
                     /locus_tag="MB219_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCC8426050.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17668..18435
                     /locus_tag="MB219_000314"
     CDS             17668..18435
                     /locus_tag="MB219_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCC8426051.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            18625..19023
                     /locus_tag="MB219_000315"
     CDS             18625..19023
                     /locus_tag="MB219_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCC8426052.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(19329..19703)
                     /locus_tag="MB219_000316"
     CDS             complement(19329..19703)
                     /locus_tag="MB219_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426053.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(19725..20102)
                     /locus_tag="MB219_000317"
     CDS             complement(19725..20102)
                     /locus_tag="MB219_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426054.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(20092..20487)
                     /locus_tag="MB219_000318"
     CDS             complement(20092..20487)
                     /locus_tag="MB219_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCC8426055.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(20554..20739)
                     /locus_tag="MB219_000319"
     CDS             complement(20554..20739)
                     /locus_tag="MB219_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCC8426056.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(20821..21075)
                     /locus_tag="MB219_000320"
     CDS             complement(20821..21075)
                     /locus_tag="MB219_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426057.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(21349..21633)
                     /locus_tag="MB219_000321"
     CDS             complement(21349..21633)
                     /locus_tag="MB219_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426058.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(22127..22666)
                     /locus_tag="MB219_000322"
     CDS             complement(22127..22666)
                     /locus_tag="MB219_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426059.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(22779..23045)
                     /locus_tag="MB219_000323"
     CDS             complement(22779..23045)
                     /locus_tag="MB219_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCC8426060.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(23047..23268)
                     /locus_tag="MB219_000324"
     CDS             complement(23047..23268)
                     /locus_tag="MB219_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCC8426061.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            23494..24312
                     /locus_tag="MB219_000325"
     CDS             23494..24312
                     /locus_tag="MB219_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCC8426062.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            24316..24612
                     /locus_tag="MB219_000326"
     CDS             24316..24612
                     /locus_tag="MB219_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426063.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(24794..25147)
                     /locus_tag="MB219_000327"
     CDS             complement(24794..25147)
                     /locus_tag="MB219_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426064.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(25167..26222)
                     /locus_tag="MB219_000328"
     CDS             complement(25167..26222)
                     /locus_tag="MB219_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HCC8426065.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            26438..26836
                     /locus_tag="MB219_000329"
     CDS             26438..26836
                     /locus_tag="MB219_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426066.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            26839..27012
                     /locus_tag="MB219_000330"
     CDS             26839..27012
                     /locus_tag="MB219_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426067.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         8657 a         5037 c         4263 g        10032 t
ORIGIN      
        1 ctgagatcac ctttcaatct aaatacacgg ttcttttctg gtaacttatt catgcttcaa
       61 ccacctcata ctgttctctc atacctttga cttcattaaa aaaaactctc gctttttctt
      121 catcagtaaa gtttttatac aactgattac aataatgagg tttttgaagt ttctgaatac
      181 ctatactaac ttcaaatcct tcttcatgtt gaattaggat tacttctgtg tctctaccta
      241 gaaatattcg atctaaaatt tttatgtttg gtttcattat ttaacctcca ctataattat
      301 ttcactcaat aaggtttcac tttatgaaat ttctatagtt ctcttttcac aaaacgtacc
      361 atgttctcta agactgtttt tagtctcaaa tatacatgtt ttttcttatc ctttccttat
      421 acactcaaaa tcaaagataa aacttaaaga ataaactctc caataacaaa attattagtc
      481 agatccactg gtctcctgat ctgaaattta ttacttcttc ctgttgtcaa atactgtctc
      541 aaatcaacag aaagtttttg atcatcttct ccttttctag caggagcaac ttttttttca
      601 tacttcatta actctccact ggtcatcttt ccaatgatag tgtaattaaa atcactttca
      661 aaactttggt aaatttttcc atctgtattg aatatatcag ctgtttcaat atcttctttg
      721 tattcattgt cttctcctga accgattgta tcaaatagaa aggcaagacg aattccatct
      781 tcttcaagca ttgttaatct ttttttaatt agcccaacgg tttcaataga taacttatcg
      841 aagccgtgaa tcataacaat atcatctttt tttgcttgtg atgtgatgaa atcaaaggta
      901 tttaaaaatt gagcttccat aacattggtt tctgctctta gatcacctaa gttatagtaa
      961 aactgtaatt tgtcactttt accgatttct ttcagtgtcg ttctttgatt aaacatccta
     1021 gagaagctgt ctaatgctgt tctcaatgtg tcttttaata caataatacg atcgacttct
     1081 ttttcagtac cagatttttt ggcttttgtt tcagcattag tcaaatagct aataaaatcc
     1141 ccaagaactg gcattgtatc actactgttc accaaaagtt ttgaacgttc ttcataatta
     1201 atatcccaaa gtttctgact tatataaaag ttacctaatt cttttttcaa taaaactcta
     1261 gttgcagaat ctaatgttct gcctgctaac aaataaaaaa tctgagttag tttatcgaga
     1321 ttccgtctat ataaatcacc aatgtccttt tgattaccaa aaagctccaa agagttaaga
     1381 ccttcttcag ccaaattaac cttttctatt acactgttta acgaaggttt ggcaacaaat
     1441 gttctaccag ttggatcacc aaagtaatca aatttattta agacaatatg aaaagttcgt
     1501 ttatgatttg ccattgcatt attagcaatc aattgccccc aaagtgatga tgctgagatt
     1561 ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg ccactaaaac tttacttgta
     1621 aaagaatcat ccagtgccat aaaagctaca ccacttgcgt agctagtaga catagtgccg
     1681 atagcccatc cattcgcatc atctaaacct ttgcgaattg catgatcatt tcctgcaaaa
     1741 tccgaactca tagacgtata ctcatagaca ttcccgattg gttcacttaa aagtctatca
     1801 aacagatcac gttgatctcc tccatatgac ataagcttta atccatacat tttttcatca
     1861 tacaaccgtt ggagtttaat tagctgttcg ctgattttat taggagaatc ggatactaat
     1921 aggatttcta gcttccaatc aataatttta tttgccatgc tgtctagtaa agatgctgta
     1981 ttcaaatcca taatccgcat aacagttttg ctaatttcag catctgaatt ttttccttct
     2041 aaattatttt cataactatt cgttgatccc ccaacattct cgtgcattat ttcttgttga
     2101 tctttttgtg acactggctt atctgcttca attagatagg cttttacccc ttctactctc
     2161 acttcaggaa tagctttaac catccaacca aatccataat ttctattcat tccataagta
     2221 ttcactaatt tcacaattgt tccccaccgc ttacctgtcc taatgtaaga tggtgtaaat
     2281 tgataaccaa ctaaagatgt taaatcacta acaaaggtat tattcactac ctttgaattt
     2341 ctagtttttt gttctttttt agtttttctc ttttttttga aatttaatag ttgtgatcta
     2401 cccataattt ccatttaatc tctcacctct atttcagtgc ataaatatca gataatattt
     2461 cttttgtttg tttagctgac aactgagtga cactataata aagtcctcta ctcgtatagt
     2521 tttctaaacg ttccacacct tcatccaata ccatcttaga ttgagatttc aagataagat
     2581 attggattac tgttgttttc acaccatcaa tatattctga tacaaatcgt tcttgctgat
     2641 ctattatgtc aaagactgct ttgttactcc ttgcatttcg tttttgtatt ttcatatttc
     2701 gagtttgtct ttccgtattc tgaggttgag atgaagtgat tttgatttca gctaaggaac
     2761 gatctctggt attttgatat tgtattgatt tcgattcctg agccataact tcttctggaa
     2821 aagcggttag tgatgtagcc ccatctagat tgaacattaa tgctacactc ccatctcgat
     2881 atacgattgt tccatcttgt ttcactttta aaatctctgt gcctcgaaat ggtttctttt
     2941 tccccataga tactcttgct gaattaacta ctgatttgaa cagtctaata gaaccagaaa
     3001 tcaatgaacc actagatgga tcaataaaca tatactggct aattagaata agaaatccaa
     3061 ttatcattaa taacgttgga acaaaaataa atttcagttt aatcgtatca gaaactaagt
     3121 aaacaattga tccaagaaca acgactgcaa caactatagc tgatacaatt aatctttttg
     3181 ctgtaacaga ataggtgcta ttgacatgct tttcaagcca ttcaactctg acaattgttt
     3241 cagaaggttt ttgatgtaag ttatgtttaa atccatagtt tgagttatct gccatcagaa
     3301 atacttcctt tcacgttcaa cttacggtaa acacctacag ttatcactat tgcaacaata
     3361 agcaagccaa ttaaagatat tgacttgctc attgtctcgt tcgtttgtgg tagttcattt
     3421 acctgataca cgagctgtgc ttcctgtaag tgctgagaag aggtcatgtt ttcttgattg
     3481 ttttgaatta ctggataaac tattttttct tctcgcataa tagccctcct tagttattat
     3541 cagataagtg agcttgaatt ttgtactgat ttccgtctgg agttttcaca actgcttttt
     3601 ctccatcaat agaaatcact tcagccttag ttgttttccc atcaatactg atgacaacat
     3661 ctttcccaac tttgaactct gtagttggtg ttgcttcata gctactattt gttgaagcag
     3721 agttcgtatt tgtcccaatc gaattttcca ctctcgaaat agatgtttga gtttgagcct
     3781 gtgcttgttg attattatga ctaataaagt aggacgatcc caagatcgtt ccgcaagcca
     3841 ttgcagcgac agaaatgata gtagccatct ttttacttgt aaaatcacgt tttgaacttg
     3901 attgattttc tgtagcaact gctaatgatt tcgttttttc tgccatttga gctagatcac
     3961 ttcgggaacg agataagtct tctcttaaag cttcactttg ttctgccaac ttttcgctat
     4021 ttaatttata accctctgct aaatctttga cctctttcat tcgatcttta tattcttcga
     4081 tagttttttg ttgctctaca agttttgttt ctaattcttt cgtgttgtta gctacctgct
     4141 tttcaacttc ttctttgttc gcaaaatatt tgacaaccac atctgttgtt ttctcttttg
     4201 tatcatggaa ctcagcttca ttttctgact ttgttttttc gacaatctct aaagtttttt
     4261 cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa tgaattgata attcgttcca
     4321 ccacaacttc ttttgcttct tggctttgtt tcaatgcatc gtttttaata gattcaattg
     4381 cttcttggta ctgttgatca agactttctt cagttgcttt tctatattca tcttcttgtt
     4441 gtttaacttc tggtaaatgc tcagcatcat accgttgctc tagtttcttt agttcagcat
     4501 ccaaataagc ctgtttcgcc atatcataat ctttacgaat gatattggat ttttccatca
     4561 ctaaatcatc aattttagat gcttctgctt cgtagctctc tttagcaacc gatagtgtat
     4621 ttgtaaactg attttgacca tttaatgagg ttgcttcctc tatccgcaaa agctcatcag
     4681 ctaacatttg ctttcctttg aggatcaatt gctccttcgt cttatttaat tcactgttga
     4741 ttttattatt agcaatagag atgcgagcgt tcatactctc tttagctaaa aacagcaatg
     4801 gtttcaactc aggtatggat agcatctcct cgtttaaatc gacagggtca ttaatctcat
     4861 ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc aaaatttttt tctaaaaaat
     4921 cagcatctaa gtttttcggt acatcacttt cagatgtgat tttttcagct tcataaacat
     4981 aattttcttt gtcactttgg gattcgggtt tttcatcaat ttttggttgt tggtaggttt
     5041 ctgtttgtat ttgtgtttct tctatgctta cttcttggtt ttctgcttcg gttggtttgt
     5101 tatcatccac aaaaagagct tctttttcat ctggtacatt ttctgctacg gcttcaaatt
     5161 caacttcttc agaagagttg aaggtctcag caattcgttc taatccatta gtagctactt
     5221 ctaattgctc tactgcgcta tagaagtctg tagcttcttc aacttctgat tcaaacacat
     5281 taggatcatc cggaataact aataattgac cactactatc cataaaacgg acacctgcta
     5341 atagcttctc tttaaatttt tctggtagat cagctacata attcaaactt gacttcaata
     5401 gaacgacata cttctcagaa tcgagaatag catcatcctc atctataact ccctcactgt
     5461 tgagcttttg tacatcttca attaccatat aatttttctt tggtaaatct ttggtcagca
     5521 acgcatcgaa ttctttccat acctttgctt tagctttttt tgccttcgct tctttttctt
     5581 cttttttgta cacttcgagt aattgtggct tcaaagagag tgcaactaca ggaactgttt
     5641 ctgccacata attaatagat tcggtaatat ctttgaattt tgccattatt gtaaaactcc
     5701 ttttttcagt caatagttct tttctgttta tctagcatat atacatatgc tttttcgaat
     5761 ttaataagcg tttcaagtct ggctttttca atctttttac tcccatttcg ataatcatag
     5821 aactggttaa cattcatatt cattatcttt gccagttcgg tacctttcag actgctcatt
     5881 aatacttcgt tggcgtgagc taacattttt tctctatcaa aaaccatctt acatttactt
     5941 tcttccctca cttctaaact acgcttggag cgtagttttt tggtaaaaag taaattgaac
     6001 aaatcggcat attatccaaa tttgttcaat tcacttttaa cctaacagtt cattttttgg
     6061 aaattttgaa actagataga taccaacaac tggaataagt gctgatatga tattagcttt
     6121 ttggttcaaa tcattacctg ttttagtacc cattgtgtaa agcataacgg ttgtaattac
     6181 aggaataata aagattgcaa taatcgcccc aacatatttt aatccatctc cccattttgc
     6241 atcaccaaaa aatttcattg cagtgaatcc tggtttcaac gaaaaaacga atgccaagaa
     6301 atacaaaaca acttgtacta aagttttcat atcaggcgtc attccgctaa ccaaattttt
     6361 aatagaatcg aacatataaa aacctccttt ttaatatttt taataagtta attatatcat
     6421 cgacaaatct tatttgttca cttgtcaatg atctaactaa gaaaaagacg tctcgatatt
     6481 tacatcttta tgttatcata ataacataaa ttttcgatga ttaaaagaga ttttccatta
     6541 aattatttca ctttttttat tggctaaaaa tgctggttta tcaagttttt gtttagataa
     6601 taattctgtt ctttaccagc ataagccctt tttaattata caactttgaa cagtaaatat
     6661 ttaattcatt tatagacaat ttaaaataat ttttttgcat aaatagaaat tttccttgac
     6721 atcttcagtt agaaatcaaa gtaaaaagaa ggagcaaatc aatgctcctt ctttttactc
     6781 gtcttcagac ttattttcag ttgatttttg attaccgtat tttacaatat agtccttgac
     6841 tgacatggta gatttcttca ctactttacc agttttggat gctcctgtag cagtagcctt
     6901 ggcaacctta gccgttcctt tacctatttt aatagcttcg tttttagctt cataggttat
     6961 ccctgctaga ctaccttttg gagcttttgc taaagcttta tctgctgagg tgtaaacatt
     7021 tttaccagtt tttgatagat attcaacggc tgatttagct gttgatgttt tcttggttga
     7081 ttgattagat ttccctgaat tatctgaagt agatttagaa ttatttgttc tattattttt
     7141 atttgtcagt tcaggttctc taattgattg attgtctttc gacattcctg atccaatatt
     7201 tgtttcatat ttatctgttt gattaacatt aggagtagga atgaactttt tcttcacatc
     7261 tgattgttct ttatccgttt cttttgttag cgattctctg tctttaagta ctgtgtcatt
     7321 ccgaatagca ctattatcaa agttgctgtt aagctctctt gactgacttt caccagatgc
     7381 tccatctcct aattctcgat gcactctgtc tgacacgtcc tcaccactca tttcattcct
     7441 cagttcttga tgagtttgat ctaaagtaat ctcgtcttct tcattctcag gaattgcaac
     7501 agcaattggc gtatgccgtt gttgttcgag ttctctattt cgatctgcca caagcgcatc
     7561 tctcgtctca atgatattcc catcctcatc gactaaatct ccattcacat tatatccatc
     7621 gctgtgttta ccaagtttat taccaaactc atcaacaaga tttccatctt catcaactaa
     7681 acgacctttt tcgtcaacta aacgtccgtt actatcatga ccaaaatcac catttagcaa
     7741 cctgcgtcca ttttcgtcaa caagatttcc atcttcatcg actaagcgac ctttttcatc
     7801 aattaaacgt ccttcgctat ccataccaat attaccatct tcactatctt ccaatggttt
     7861 accattagta tctataaggt ttccatcttc atcgaccaaa cgcccttttt cgtcaataaa
     7921 gttgccttta gcatctttac cgtatttact atcttttaat atttcaccat tttcatcgac
     7981 aaagtttccg ttttcatcaa ccatacgtcc taaatgatca atatattgtt gggagttatt
     8041 acgtagttga tcttgacgaa ctaaatcttt gaaagaggta ttattgattg tggaaccaac
     8101 tccgccaccg ttgaatgaca ttccttctgg tgtttcagga attactttat cagcaatgga
     8161 cttagcatca gcattgctat ctcccatatt gttcaaacgt tcagccattt tttgcgctcc
     8221 aactgctcca agacctcctg caaccatatc ttttactgag ctgttgccac catcataata
     8281 gggattttta gctccttggc ttttcttgat tgctttagaa acgccgtcca caactcgcac
     8341 agcatcattt ctattaccaa ataataactt agcacgttca tcaaacccaa acgctacttc
     8401 aagtggtaag ccaataagca tattaatcaa attatttact ttttcgcctt ttttaaagac
     8461 taaaagatat cctacaccta aaatcatagc agatcgaaca atatttgcta cgccatcgcc
     8521 aactaatgta ttaggaatga atcccacagc agtatcacca aatgcacgaa taaaagtaat
     8581 cagaaagata ggtaattgta gtccgatcag agatgttgta acaacaccta atgaaattgc
     8641 catcacacct gctcctgatg caagtgatcc tgttagaact acttcccaaa tccgaataaa
     8701 ttcacgttta aatgctttca cgatattcac attgaacatc gcccaagcac atgctaagta
     8761 aagaactgct aaaacgattg attcagcgtt catcgctagc cacgagccaa acttacccat
     8821 agctccatga ccaactaagg atgctgtacg ccattctgtt tttactgttg acatattctt
     8881 agcactgcct tgaattcctg aaggagcgat attatacgca taaaacttag ctcccgatgt
     8941 atcaagtgcc gattggagca ataaagctac cgactgtgtg ctaaaagttg cattgttagc
     9001 aacgccccaa ggttgagtag catcaaagtt atgcgctgtt ccttttccat caggcttgcg
     9061 attgtcgtct gtcacatttt gatttgctga ccaaatatat ccatttaaat tggttggttt
     9121 cttaattgca cttccttctt ttccatcccc taaattatag cctgtccaaa cagcaggcgc
     9181 tcttcccata taagcatcat tttcggcgac attaacatca ccggcataag tattaatatt
     9241 gaaagtatca ttcgatgacc attcttttaa tagagccata ccgactgaat tagaatctag
     9301 atcattattt tgtctggcat aactgctagt attgatcgct cttatcattg cagatttttt
     9361 agatggttca taatctgtat caacgaactt gatttcggat gtaggctttc cgtcaggtac
     9421 atcaccacca gtatcagatg atggagcaag attttgagta gaagcccaaa gtcttgtgtt
     9481 aatgatgtag tcagaaacaa catttgttgg tatatctcca tctttttttg ctgactggat
     9541 gccgttgata taaactccca atagcgatcc tgccactggt aagaatataa acaatacaaa
     9601 aatacgaaca atccattttt caagtactgg tgataatccc ttcgagttaa caccatcttt
     9661 cgacaataat ttcattcctt gataacctag tattgctgtg aaaaagagca tagataagct
     9721 tgctatcgct ttaaataatt cattatttaa tcccaaattg ttaagcaagc tagttaatgc
     9781 tttagatacc ggattagata catccgatcc accttctccc caccccataa gagaaaatat
     9841 attcatactc atgaaaaatt ttccaaggag ttcatttatg gattcagcaa tattagaaag
     9901 gattaaacta attgatccga taaaactacc aatcgtccta ccaatacttc ctagaatgga
     9961 tgatgcttga ctggcaatag catttcctga gactgtttca atatatccgt agtaactata
    10021 agttgctaaa gtagatgcta gttgagtacc tgcattaccc cctgcaatcg aattaccttg
    10081 tttagaaatg tcagaataag ggaaatcggc actgatacca ccttgtccta acaaattcat
    10141 ttgtccagtt ttagcggcac tatcattaac ttctttatct cccattagga tacgagcata
    10201 tagtgtccac attccttgtt ctgccggttt actttccgca tatgcaacgt tcttatttat
    10261 aactgtttct tgtttgctat cttgattgtc aggagttgga tcattcattc caaaaggagc
    10321 gcctaaagta actgctaata ctcctccaat aactagacct cttacgaccc atcgttttat
    10381 tctaaggttt tgtagtaaat atttcttgaa tttcatagct acgcccctcc aatcgaaaat
    10441 acaatcttgt tttctttact ttcagcaacg gaaggctttg caataatttc agcattagaa
    10501 tcaccttttc cttcaaacgc tccggcttca ctcaacttaa catcgaatat ttttgcgttt
    10561 ggataagtaa ttattgcttc agtaatcgct tcttcaaact cagattcaac taaatgcaag
    10621 atattccttg caccccctga tttagaatcc tcttgtgaag ccatgtcaaa aactaagtgc
    10681 atggagagat cgtctgcata aaaattttga ccatatactc tttctttcct aggtggaaga
    10741 cgtaaatcta tatttaaatg ttttcgtact aattcagcac tcttcaacat ttcattataa
    10801 gcaatttcca gaagagtttg ttcggataaa gcccaaaatg gaactattcg tccaaatcgg
    10861 ttaataactt caggctttag accttcacca atcaaggctt gtttgatccc tgatgcgact
    10921 tttcggtatt ctctttttag ttcagtgcta tattcattag aattttcctt gtttgtaaca
    10981 cgagatagtg agctatatct gtttaacgaa tcgaacatct ccgcacctgc atttgttgtc
    11041 gcaaataaaa ttgtatttga caatgaaaca gtgtcgtgaa cttctcgatc atcccatcct
    11101 ctttcagtaa aacttatcct accttcatct aaaattgata aaaaggcatc gagtgtttct
    11161 acaccagctt tctcatattc atcacacaca ataatcgcag aaggatcatg ccttacatga
    11221 gtacctatga gtcgtctaaa acgcattgca tcatcttttg ttttgtagtc cggcatatta
    11281 actaagagaa ctttattttc atcgccataa agtcctcttg ccacggcttt agccattgca
    11341 gttttaccga cccctgttga tcctgtaaac agagcagttg cttttggttg ctttcgacct
    11401 ttaaaacgat caaatccaag cttcatacgt ttaaacaaca ttgttatagt ataaactgct
    11461 aacggttgcc cttttatgga ctttaattcc tcttgaagtt tttccatatc aaaatcagaa
    11521 tctagctgaa tattatactt accaaatatt tgcaatagac tttctcgatt ataagcattt
    11581 ccgtctactc gatcttttgc aatcaaatcc tccattacgg caattgattt agccggttcg
    11641 gacaaatcct cacgataaag cttgtttgct tgtacgattt cttgaagtaa ttcatcagga
    11701 atcctttgcg ataatttaaa agaagcctga ggatcatctt taacagagga tgtatttagc
    11761 cattgtctca aaatatcgac gacaacatcg ctttttagtt cattcaattc taacggttgt
    11821 aaccgtctcc ctaaggcatc atctcttttg atatagtttt catattcttt tcttgtagta
    11881 gcggtaatca attgaagtga ttcatttgct aacggttctt ttagcgaatc acccccaatt
    11941 ttattgccac taccaaaaga gctaacaact ttatgaaact cgtcaatgaa gaggacaaat
    12001 tcattttctt tgtcctcaga ttttaaaatt cgctcatatt ctttcaatct tggaattaat
    12061 gtttctaaac gtgtttggag gacatcatct cccccaacac gcaattgtcc gattgataat
    12121 ttgtatactt gtactttctt tccatcttgt cgctgtcttt cagcccattc acttgcaagt
    12181 gctgttttac ctgttccggc ttcacctaac aagatagctt gcggtgtttc cggtctattc
    12241 aagatcagtg acaactcatg gagttcgtca tcacgtccca ctaacttatg tttcggtggt
    12301 ttcattggtg tgagagcatg attaaattca gtttgaatat tcttaaaaaa ctcggtacta
    12361 ttttccatct gtttgtgttc acttcctcta aaataagaat aatttacttc tagtcagtca
    12421 gagttaggta cttaaatgtc gtcttcatgt ctccaaaaga atctttgtta aagtcagcat
    12481 ttttgtattg atcaatcaat ttttgtgtat cattgattgc tttaatttcg gtctccattc
    12541 gactgtctag ttgacgaata ctttcttgcg aatctttatc gtcaggattt tgttgtaaac
    12601 gttccagata ctcagctttt gtagcttcaa ttttcttgat gttagtattt gaagcattta
    12661 actgatcttc taacttactt acaacattat tcttacttgt aactgctaca acttttgaat
    12721 aatcaatatc gccatctttg gtaagaaagc ttccttgata gacattagtt gaatctgaat
    12781 atggatttat tctgaaattg atattatcag gagtattaac aggttttgct tttttcccaa
    12841 aagaaattac tccttcatta ttaacatcat tcatagaata ttgagaaagt gcttttgtaa
    12901 ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc agctgtatca tcaccagaaa
    12961 ttagtgatac ttggtttgca ataaaaactt gataagctct ttcatctaat ttacctttta
    13021 ggaataagaa agcatcccca tcagtactta acatcccata gctcatttca agctttttag
    13081 gacgatcttt ttctttcgtt gccatatata agttgtagtt ttttccttgc gtagataaca
    13141 actttctagc gttttgcgag taaccgagtt ttactactgt tacatctctg ttttgatctg
    13201 tccacacatc tgaaatagtg atctctgctc ctgatcgagc aaactccaat gaagagccta
    13261 taggtgttgt attgttcacg agataataat tatagtttcc taattgataa atgtagctac
    13321 ccaagcctgt taataaaaaa agcactccta gaaaagttgc aagcataatg cgtaatccgt
    13381 gacgtgttct cttatcctta tcaccaagca accctccaat aatactgtat tgatctttgg
    13441 tagatgttga attgacgttt ttttccatta gtttgtctgc cccctttcat aaaagagcaa
    13501 acaatcagac gttttttcgg taattctagg ttcgggattt tgaatcattt cggtatattc
    13561 ttccccatta atatttaaca caaaaattat cttcctttct tcgttccttt cttttgctct
    13621 ttattttcca aataatcaat gatttttgct attgtaccaa gatagtaatt cccctcggct
    13681 ttgttttttc ggattttgct aaccattgga gtaggtacaa tctcttcatc tctcaattca
    13741 ctgaaagatt ttttacctag atattttcca ataacagatt ttaaaaaacg ttcaaaatct
    13801 ttctcgtaag ttgttttctt gccttttagt tcatggttca tattgtcaac gacttttgcg
    13861 gtttcaataa ttgttttagt tctcagctta ataatttcat ctggaatttt caatcctcgt
    13921 aatttcatcg ttgtactaac tggtaaccca tgctttactg catcagcaat actaaaactt
    13981 tttagatcat ctagtaagct ctccatatag ttaacaaatt catgccaatt aatagcattc
    14041 attgtctcac cccttcattt ttttaaaatg aggttcgggc gtaacacatt tatgttatta
    14101 taatatcata ataacataaa taaatcaatc agcaaattta gatatccctt aaagtatcag
    14161 caattatcca acgagatatt tattttagct atttcagtca tcatgaaaga ctaattagca
    14221 ataaaataac taatattatc catttatcag acgaaaagaa aaaacttatc taattactct
    14281 atattaactt ttaacaaata gttgacttgt ccaaaaatgt taccttcaat ctcacaaaga
    14341 tattaagatg tttcctacca aaactgatat caccggactt tttattctgt gttgtccaaa
    14401 agtgttacct ttagtcgatc aacaaattaa gaaaaattgt ttttttcttg acttgtccaa
    14461 aagtgttacc catagttgtt aaaatttgta tagattagcc attctttcaa atattattta
    14521 gactgaaact tgcttacaaa aaaacttctt gcatttattg tccaaaagtg ttacccatac
    14581 ttttttaaag aagcaggttg tattaaaatt acaacctgct tcttctcaat cttcttctaa
    14641 ccaattatgc attgaaatgg ttggcaagtt tgattctggc tcactaaaag tgaatctgag
    14701 ccgatcaact cttcttcctt tctttgcata gattttttca taatcaaaag tttcaaatac
    14761 ctgtattccg ttttcatcag gagctaaaaa ttcttctttc gcctgctcta gtattctttt
    14821 atcaatattt gtcattctat aacttgaagg tatacttaat gctcttctaa aatcttcaac
    14881 tttgactacc caataaccat ttcgccactc tttatttctg tattgcatga gatgacgata
    14941 caattctttt gtgtagctac ttcttagttt tgtaaaatta tcaagttcaa aacgagtaaa
    15001 gtttttgcca attgagttta agataaattc aaattcctca tttgccttaa ttgtaactgt
    15061 ttccttctgt ttatctatcc ggaatttttg aaaaagccac agctgttcat agacgtctga
    15121 atcttcaaaa cggtaggtta aattacctag tttgtcactc aaatctttta acacagaata
    15181 aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa cgaaagaggt tgaaatcaaa
    15241 agttacttct gctgttcctt gacgtttcat ttttgataaa atagcccaaa acacatccat
    15301 ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga ccattgttat aacgcacaat
    15361 tgcatttgtt tgttctgata ctaaatctac atcactatca ataatctttt cggtaacttc
    15421 ttgagacatt gattactctc caatcttctt tttgcgctcc tttaatcatg ttaatctggt
    15481 actaattaaa ggaaacaatt ttttctgctt gttacctaaa ttaaatcaca actaaaggta
    15541 acagtcaaca aagatttgtt actaatctaa ttttctattt tttcgccttc caaatcaaga
    15601 aaaccgaagg taacgtctta tcctagtgtg atacctcaaa tgtccacttt tgttaccttt
    15661 gctgtccaaa actgttaccc ttactgtcca cttttgttac ctttactgtc caaaagtgtt
    15721 acctttatac ccctttaata cttactctcc caagagatta agcctcccct aacagatata
    15781 ttaacagata tttaacagat atattaacag atatttaata aaggaggatt actcctctct
    15841 tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact aacactatct aggagtttat
    15901 tttgcttgtg taactttgaa taattgaaaa aaaggaaaag gaggaagctt cagcttcccc
    15961 ttttatttat atttataata tatattttaa tctttaaaac tttagcaacc tccaaaaagg
    16021 ctttatatca atcttaaata gattttaggg tggacataaa gcgatgcgaa catggacaaa
    16081 aagcgatgtg aatgtggaca aaaagcgatg caaacgtgga caaaaagcga tgtggtattc
    16141 cgaatatatc caaaaaaaga aaaagtatgt gaatttattt ctaaaaacta gaaaaatatt
    16201 tgttttttaa ttttggtaat ttttataatt tcgtatttta ttgaaaagga tttttatttt
    16261 gaaaatgttc taaaatagcg atttctgctg tattttcaat gaaatttgat tggaaaaaat
    16321 aaaatgtgtt ttactgacat tgagttatgt tataaattta cgatacattt cttattgtaa
    16381 cgtatcgaaa acaggtctat acttattcaa tggaatcgta ccagttccaa gtttttaagc
    16441 tcgtaccaag cttagaaaga agaggcaatc gtatgaaaat aacagaaaag aatgtaaacc
    16501 cgaaatttga aactaccgaa agcaatctaa aaaagtttga tttaactgtt actttatcga
    16561 atcaacttgt agatagcatt gttgaaatgg atgctatgcc acaaaaaatt tatctttacg
    16621 ctataggaca tcttgaccct aaaaatccgc cttttaataa taccgtcaaa gtatctaaaa
    16681 aggctttatt tgcgttgatg tcttctagtt catcttcttc aaaacattca agattaaaac
    16741 aagttattga aaaattgcat cagcaatctg tcattaaaat tcaaagaact aaacctaacg
    16801 gtaagactga atacaaagtc atctcaccgc tagaaattac tgaattcaat gatttttctg
    16861 atgcagtatc tattaaattt agtagttcaa ttatgccttt attaacgaac ttagaagatc
    16921 attttacgca atatacattt gatcaagtag ttaacttgac aagcaagtat gcgatcagtt
    16981 tttttcaatt attcactaaa gattttaatc tgtatgaagc aaatagagat aacttaacat
    17041 ggagtacaaa atcacttgat agattaaaaa atcctgtttt ctccattgca gatttacgaa
    17101 agaaatttag tattgagaaa aaatataaag cttttcgaga ctttgaaaga cgtgtaatta
    17161 aagaaccgat tgcccaactg aataaataca ctgattttaa aattgactat gagcttatca
    17221 agaagggtca tacttataca aacataaagt tcttgcttac acgtaccatg tcagttccgg
    17281 taccttataa gcaagaacaa agagaccctg cttatttaaa aggcgcagaa gataaaacag
    17341 agaaaatgaa tcaatcgtac ttgaaagcta tgaatagcaa atacactaaa ttactaatca
    17401 aaaacttttt gatcagttct gccgacttat gcgatcttaa acttatgagc cgacttcaga
    17461 ataaagttta tccgctttat gatcgtttat ccaaagatag aggatttgaa tcagttgtca
    17521 atcacctatc ttacgttgct actcataagg aggattacac aataaaaaat actgttaaat
    17581 atctcgaaaa atcaattacg agttatttgc cttatgtgaa cctttatgat taacaactga
    17641 aaaaatgcgt gaaggaagga aaaacttttg aaaaaagatg aactgacaaa gtacaatgag
    17701 gagttcttag caacaattga tatgaaaatg gatttaaagt cgtatgaact ttatcaattg
    17761 ctgttcagtt atatctcacc cacaaagcta aagaaaacag gcgtagtaga tttacctgtt
    17821 aaagcgatta ttaattacat ggatgttgat caaaatcttg aaacggaact aaaaaccata
    17881 gttaacactt tgcaaagaca agcagtttat gaaatatcga atctagacaa gaaatataag
    17941 acgtatacta ctgtatctgc tattgatcgg attcattggg atttagaaga gcatagtgta
    18001 tctttcagtt tttcagaaga tgtaattccc ttaattgtct caatcaaaaa gaagtatccg
    18061 ttctttgttt ttaaagactt agcatctttt caaaaaaaat atagcatcgt gttatacaaa
    18121 tatttggcga tgtattataa aaaatatctt atcgccaaaa ataaaggaga ttttgagaaa
    18181 gctgaaatat ataagaatcc cctgattgaa atagttaaaa ttcgtgagat tacgaaaaca
    18241 gaaaatgagt ataaaacaat atcgaatttt gatagacgtg ttttaaggga atcagttgaa
    18301 gaaattaatg aaaaatcaat gctgaagatt gattttgcta aagttagaaa aggcagaaat
    18361 tacaattttg ctgtccagtt taatatttct agtgagtttg atgaaaaacc ttatagagaa
    18421 aaaaaattag gttaatgcaa aatatatctt actcgtttga agatgcttca gacgtatgtt
    18481 aagatatgta gtgccttggg agcaaggtat taatctcctt tagaggttaa ttagcttgtg
    18541 atcgcctatc aattttttgg taggcgattc tttttttgaa accaaaaagc aaaaattatt
    18601 ttttaattag agaggtgcaa tgcgatgaaa tacttttttt gtgcagtatt cgaatggaaa
    18661 ggaaatgaat atgcagttaa attcccagat tttccagact tatatacgca tggtgaaact
    18721 ttagaagatg cttttgagat ggcgcaagaa gtattggaag gatacttggt gcttttagag
    18781 ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag agttaaatgc aagaatcaag
    18841 cgatctgaga atgttaattt atttgtcgta aaagctaata cgaaagttgc taaagctcga
    18901 aacaagcaaa attatgatcg aaaatcagtg actgtggaac actatttaaa tgttttagcg
    18961 gctgaagaga atattaatct gtcgaaagct ttgaatgatt tgttgagaga aaagttgatt
    19021 tgatgtctct cagatgatca tattaagcaa aaacagcttg ttttaggacg tatggattta
    19081 agaaagacaa attatcaaag aatatattaa aagctctcag aagcgttctg agagctttta
    19141 aattgctatg tattatactg catctgctca aattgatttt cttttcatgc ttaccatgta
    19201 tctagttttc catcaagtaa tttttagata tgtattgagg aataaatagc aaatttgctt
    19261 gatataagct cgaattaggt gctttgtaat gtgttaggat aaattataca agaaaatgtc
    19321 aaaagctctc agaacgcttc tgagagcttt ttatcggtag gcttgtttaa cgtgatcttg
    19381 aaagataccc ttttgatgtt tctctcgatc cctgcatcgc taattactaa gtgttcttca
    19441 agtgtgatgt aggtatataa attaagcatc aaaatggtgt agataagcaa aaaactccct
    19501 aaaactaagt gttgattata aaaatctgac ttataaaaaa gaactgagaa tgttagtgtt
    19561 gcttcccaaa aaagaaacaa aattttgtat ttccaaagat agtggatcaa gcgctgtgct
    19621 ttactcatct caaataattt ataataacct gctaattgat cagcaagcgt atcgttaatg
    19681 ggttctgttt gtgtttgttt catctttctc ttctcctgtt tcaattaatc taaaatttca
    19741 gattgagcaa gcaactcatt gtggtagtcg gtttgaaatt caaataacaa tttagccttc
    19801 ttaacgaggt ttaaagcgtt cgttagtgat tcacttaatg cttcaacatc tttaactgat
    19861 tttgcatgtt tcattttttc agtgtgcgta tagcagaatt caggtattga gttaggagtt
    19921 gcctgttcaa taaacatctc aattcgcaag ttattatcct caaaaataat tagatcattg
    19981 gcaaagtgtt tggtactaac gctaatgcca gttgtatctt ttaatagatt attgattaac
    20041 gttcgagaga aatagtttaa gtgtgtagat ttcataggtt cggtctcctt tttacttttc
    20101 aagcaagtct tcttctagtg ctttttgaag taattgagag aagtttatgt ttttctcctt
    20161 ccctaaaaga actaaccatt ccggaacact aacatttttt ctaattgttt ttgtatttaa
    20221 gcttcttcgg aaaagtttaa tgtttagttg gatgattact actacttgat tggctttcaa
    20281 agttaattct tccactgctg atggtttagg aaattctgca tctggtacac tatatagcgc
    20341 taatcctaaa gcatccttag cgtttaaata tgcttcttca agactatttc caaaggtaga
    20401 tatttccggt aagtcaggga aaacgatgtt ataagcttct ccttctttct cgaagattgc
    20461 cggataaaaa atattttttg tcttcatttc ctcgctcctt ttctagtgta agagaggata
    20521 gtatgcctaa taccaaatag gaaggcaagg ctgttaaagc cctgcttgct tccagatatt
    20581 agcttctgtt cctagtggta catcaccatt gtggatcgga acaacgaccg ttcttttagt
    20641 cttggaatta taaaattttg catgactgcc agtttgtcca gtctttttga agccgttaga
    20701 tttgagaatt tttacaattt ctcttgctgt cttaggcata gctaccctcc ctttacaaaa
    20761 tctattatac acattgttgt gtatggaatc aatagaaaaa gaggatgttt tattgcaaaa
    20821 ctaataaaat gatttattgg ttttttcagc aaattgaatg ttattagcgt tgcaaatatt
    20881 cataatttcc caagtatcat tagaagtata ggtatctaag aattcattga cagttacggt
    20941 tgattcgtat ggttcataag tcaaagcgtt ctccaagtct tttgatgaaa tgatcaagac
    21001 tttttcaatt tcctcccctt tttcatttgt gaaaacgtat tccaaagtga tttgttcttc
    21061 atcggtataa ttcatattgt gttctccttt cttgtattaa gaaaaatgga tatttccaat
    21121 tgcatcccac tcggcaccat tgtggtataa gacgtgaccg agcgttacgc ctttgaaatg
    21181 ctcttctaaa tacatctaaa cgcattcaag gctagtacct gctttgagat tgtagaagtc
    21241 tgcatccaat accttttcag aagttatcgg atcaatgcga gttggtacct caataaattc
    21301 agtccaaatt tctgcaaaag tcttaccttc caaagtccac actccctatc aattaattcc
    21361 aaaagcaaat agagaaatca agcgaccgtc aatagtcact tcaacaactg aaaatgccgt
    21421 tgaaacagtt ttaacatcca cactttccaa tttccctatg aaggaatttt tgtgctttag
    21481 aatagctttc tttaactgtc tgttatacgc ttgacgactg ttaaactttt tgaattctcg
    21541 atccttgatc agctgtttaa cgacccctgc attaattgta tagctttctg caatagcaac
    21601 gtgagtaggt tgctgtaatg tagctgttag catattgtca ctccttccta aaagttaatt
    21661 agcttgttta caattacatt atacacacta gtgtgtataa tgtaaagaga ggttttttgc
    21721 tgatggatca gctgtgctaa acaaaagaaa acgatagagt aacaaaaaaa gtcagcaaag
    21781 ttaaaaagga ggaaacggct agtccggttg ctttgcttgt gtaagtttcg gctttcttat
    21841 agatcaaata ggaaattatc agtgatattc cagtaaagaa taatgtacta gctgtcacta
    21901 aaagaaatag gttaatcata cgatacactc cttctgtaat attggtatca gatacgttta
    21961 aatgcgtttc tgagctgttt tggatcaatg gatgttttag ccctacccta aaatcaaagc
    22021 ccagaaaagc atttaaaaac gttcgtacct aatacgttta tattcatata catatacata
    22081 aacataaaca taaacgtata cacgtttatg tttatgtatt taagttttag gctacttcct
    22141 taacatctcc tgctgatagt cgagtgaacc gagtgagtga tccatcttgt agtctaaaaa
    22201 taaagctacg tttgagttta tactgataaa cagcgacata cgatccatcc cagattgagt
    22261 aagttttacc tgcaactaat aagtcatcaa ggtttggttt attgttttct tctttaattc
    22321 tatctagttg ttgcttaacc attttttctt gatcaccgac aaagaatttc aaccattttt
    22381 tcggaatttt cttaaagtaa gcaggactgt catttaagaa gtagattggt ttgtacattt
    22441 catctcgaaa gatgaagctg tcaatatcgt aggttccttc tgcatctttg aaaaggataa
    22501 taccataggt cttcgttact ttggtatcta cgatttccaa accatttttg gcgtaccctt
    22561 tcttttcttc gatcacttca tttgctgatg aataagcacc ataccatccc ataaaaaatc
    22621 gctccttttg aaagttaatt agcttgttta cacttatatt atacacacca gtgtgtataa
    22681 taacaagagg gtattgttct tttttaggta aagtgggatc gtaaacagtg attttgttgg
    22741 gttaacaaaa aaacagacaa gccttatcag cttgtctgtt acttataaac ttcacttctg
    22801 tgaccaatag acaatataag aattgtcact tcatgctcgc taatatcagc tattatacgg
    22861 taggcaccta ccctatatcg ccagtagcct gatttattag ctgttagacc tttaccatgt
    22921 tttcttggat catcagtgcc tacaagattt ttctcgatcc agtttaggat aacctttgcc
    22981 tgaaacttat ccattttttt tagcgctttt tgagcttcac gttcatatct tacttggtaa
    23041 gtcatcttat aagcctaact cttttagcat atcttcgtgg gaaatacttt gatcgttttc
    23101 ctcatgctct ttcattagtt ccttgtaggt ggcaaagtct actaacgttt ctgctgtttg
    23161 cagggaatta ctacgcataa attcagaaat gttatctccg ttgaattcag caactttttc
    23221 aagaaaatct ttttcttctt tagatacacg aatacttatt actggcatag tttatcaact
    23281 ccttttcaat tacattgtac tacgattgta tgacaaatgc aatagttata tatatgaata
    23341 tatacataaa cataaacata tacatatata tgtttatgtt gtagaatata aaagcaaata
    23401 taaatataaa cacgtttatg tatataaaca tatacataaa cgtgtttata gtgtataatg
    23461 gagttacaaa aggttaataa gggaggttta atcatggaaa agaagttgtc aatcattact
    23521 ggaaacattg aaaaaggtgg aacaggtaaa acaacaacaa tctacaatct aggtggatat
    23581 gctgtcgaaa atttaggtat gaaggtgctg tatattgatg aagataagtc gcaaaaccta
    23641 tcaaaacgat ttcctgatgc tttcagtaaa gcgaaaagag agaatacaat caatcattta
    23701 tatcttcatg gatcagctga acctttagct gtaaatgaga acatggatat tttagtagca
    23761 ggtgaagatt taaaacaagt tgagatgcaa atgcgtgata tgcctaacaa cagaacccta
    23821 ctgtttagat ggatggttgt taactatgaa cagcttaaag agaagtatga tctaattctg
    23881 attgatactc ataatgatgc aggtctacta gctcaaaatg cttgggctgt atctgatctt
    23941 gtcgtaggca tttctgatcc atctaaagat ggttttgaag ctttgctgaa gcttggtaaa
    24001 gatattgatg cactagcaaa acagttgatt gatattaaga ctggtgaaac actgatggaa
    24061 actaaatacg ctacgatcgg tagcaggatc aaacataata ctaattcaag tcgtgagttc
    24121 ctagaactgg taccaacgtt atctaattat cttggatatg tacaagagaa ggaattgctt
    24181 aatgcaacta gcttagaagg taagaatatt gctgagttcg ctaaggataa gaatatctat
    24241 cgtaagaata aggatttctt tgaaagcacg tttacgctat acaacaaaat aattgatacg
    24301 gcacaagaat aaggagtgat tgaagtgaaa gaaaatagat ttaaaaaatt cgctgaagct
    24361 gaagtagaag aagttcctga agtggttgaa gcagttgaag aacctaaaca aacacctaaa
    24421 gaaacaacaa gttctttggt agctaaacta aaaagtgata gttctgaaaa gaagaaaaat
    24481 tatcaattca cgcttcaacc atcgcaaaga aagaaactat ctaaaatcag cgaaaagtta
    24541 ggaattcgat cagattcagc tttacttggt gaaatgatag ataacttgta tgatgaagtt
    24601 tttggtgagt gatcagttta aagctgatca ctttttttat tggaataagc ggttaggtat
    24661 agcttaacta aatttaatat acgtgtttat ggatatatac gtatacatgt ttatatacat
    24721 gtatacgtat atgtttatgt ttatatttgt acataataaa aaggctaggt attgtaacct
    24781 cgccttttta tttttaaagt aagtctttgt tacttggtgc attgaaccct ttcagcattg
    24841 gcttttcttt ctttgctgaa tgatcggatg taaccgttga aggatgctct tgttgaatag
    24901 ggttatgatc agttggttta ttctttactt ctgctacttt gctatcgttt ctatcgttgc
    24961 ctgcttcaat aattgtaact tcattgttct ttacacgccg atataggttc aaagcttttt
    25021 tgatcgtctg agagtattta acgcctagtg tttcatattc ggattcaaga aactctgcta
    25081 aaggtaactc gctaaccttt tctctattga ttataactac ataacgctct gattttccct
    25141 tattcatgtt gatctccttc gctgttttat tgagttaaac caaacttgta aaagcctttt
    25201 acattagaaa attcactgtc ttcaacaaac aaggtatgcg gaattttatc ttttacaagt
    25261 tgtttgtcga taatagaagc tccaccacca gtaaagacaa ctttatctaa ggttgtgaag
    25321 tttttgaatg tagctaaaac ttctgcaaca accatattgg tgtatgtttt tatttctctt
    25381 tcaactactt ctgaaatatc aaaactttca tttccgcttg gcttaaatag atatacgcct
    25441 ttttctttgc cttctcggat aatcttttca agttggaaag cattaggact gatcggaagg
    25501 tcaagcatat ttacgatacg attatataaa gtaaaaatgc cggtatcctt ttgttcacga
    25561 ttgttttcgt caatttcaaa attttggaca acatcaaaaa gtttcgtgcc accgccaata
    25621 tcaacaaaac cgatcttctg actgatcaat gtttcatctt gtacactacc atcatcagca
    25681 agtaggaggt tatatagtgt tccaatcggc tgtggcagta cccaaacggt atctacgttt
    25741 atattataag ctttaccgtc aacttcaact gaatgttgtc gtgtggcttg ttgcatcacg
    25801 tatttgacga ttgtttcggt atagtcggat gaaggcagac ctaaaactag attagcggtt
    25861 aaaacgtcat cagattgatc ttctaaacgt gctaggttag ctaaagagaa agataggata
    25921 ttttggtagg tttcctgctt gtatctgctg atcccaaaac ctagtgattc tatcacgtag
    25981 tcatcttttc ctagctcgtg tatatccttc ccaaagtaat agctttcagt agtgtttact
    26041 aggctatatt cagaaacgtt tagtgatcgt tttaatgagg aacctatatt agaaaaacgt
    26101 cctgaattaa tgatattttt gttcaaaata cgagagggaa gtgagagtgg agaaacgtgt
    26161 gtactcattt cttttactga tttattccct aagtcaacgg aaaacaataa tggttctttc
    26221 atggtatgac ctctttctgt ttgtgtttat ggtatttcgg tacgaaaata gtttacacct
    26281 atttactatt agttgcaact gtttataaga aaaaaatgca atcataaaag tctaattaat
    26341 tataataaag tgtaaataaa aaggtgtaaa taaatgcttg caaatttatc ttattatgat
    26401 attataaaaa cataaattat attaaggagt gatcctgatg cgattgagaa gaaataagaa
    26461 ttacactacc attattttag aagattttac tgatcaagaa ttacttattg tcggtaaaac
    26521 aaaagcagat tttatcgaaa agggaaatgc ttcaatagta aaagcttcaa agattgctgt
    26581 cgaaaaatat tttgattctt tagaagaaag tgctaaggaa atttttaatg gagagaaagc
    26641 tggtagctat gagttagacg gtattagatt tagcttgatc ccaacaactg aaaatattat
    26701 tccgaagaaa ttatattata tcttttcatc tgaaaaacat acttcaatga agccttgtat
    26761 ttttgattgg caatggttga ttgatgctgt attggatgta aaatgttcaa aatacaatat
    26821 tactttgggg tgttaattat gagtatgcta gttagaaaat taccattatc tgtaagagaa
    26881 tttctatttt acttggtctg cttaatcgca tcagggttta agctgatcga acatattttt
    26941 atctttgctt atgtattagc aatgatcgca tttttatctg gcggttggtt aattatttat
    27001 ttgttaagct aagttttaga tcaaaaataa ttaataaatt aaacaataaa ataaccgtta
    27061 ataaatgtaa tgaaaggaca acgcctaaca atcagcttgt aataagatta taaaataaca
    27121 ttatattaaa ataagtgctt tttaaaggac aaattaagtg aatcaacata aacgtgtata
    27181 tgtatatgtt tatgttgata cttttttgtc ttaatttttt tatttttaaa attctattta
    27241 ctaatgctgt atagatagtg atcagaaaaa acaaaatagc gaggttaagc tatttattag
    27301 ttcatatatt atattcagat taatatgatc cagtattgat ctgaaggcaa aaccaatata
    27361 tttctacgga ttcttgtaaa actatttaaa ttttcctgct aataaaataa caaaaataga
    27421 atatttagct tttttatacc attttacaat aacatgattt tatttgtaat attaaaacat
    27481 ttaaattttg attttttttc aaattagatc aattaatatc cttataaaag agcataataa
    27541 atgattttta catcgttatt tattgatatt aatctcatcc aaaaaaatga aagcgcatac
    27601 tttatgggtg attaaatgtg attttaatac atataccaat taaaagaaaa cttttttaga
    27661 tttttactta caaatttata aaaaaacaag ataattgata ctttgatttt gcgaacattt
    27721 tatctttccg cctaattcag ctgcaatatc atgattatat cagtaacaaa tcgctttttt
    27781 agctaattta agcatcaaat aattattctt aatcagtgaa aaaaaataac gaataaatgg
    27841 attttgggaa taggtaaaaa cttgatcggt ttctggataa aaataacatg aaaaaggaaa
    27901 tataaaataa catggagcgt gggaggaata aagaaatttt acactcgagg tgcgtaggaa
    27961 tgtaaagaaa cttaacacga aacaaaagt
//
LOCUS       DAHEYX010000011        26212 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHEYX010000011 DAHEYX010000000
VERSION     DAHEYX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.011"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..733)
                     /locus_tag="MB219_000331"
     CDS             complement(<1..733)
                     /locus_tag="MB219_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCC8426068.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTF"
     gene            complement(737..1126)
                     /locus_tag="MB219_000332"
     CDS             complement(737..1126)
                     /locus_tag="MB219_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426069.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1261..1803
                     /locus_tag="MB219_000333"
     CDS             1261..1803
                     /locus_tag="MB219_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCC8426070.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1951..2583
                     /locus_tag="MB219_000334"
     CDS             1951..2583
                     /locus_tag="MB219_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426071.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2717..2842)
                     /locus_tag="MB219_000335"
     CDS             complement(2717..2842)
                     /locus_tag="MB219_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8426072.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(2976..3869)
                     /locus_tag="MB219_000336"
     CDS             complement(2976..3869)
                     /locus_tag="MB219_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCC8426073.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(4051..4977)
                     /locus_tag="MB219_000337"
     CDS             complement(4051..4977)
                     /locus_tag="MB219_000337"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCC8426074.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(5062..5823)
                     /gene="pflA"
                     /locus_tag="MB219_000338"
     CDS             complement(5062..5823)
                     /gene="pflA"
                     /locus_tag="MB219_000338"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCC8426075.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(6067..8298)
                     /gene="pflB"
                     /locus_tag="MB219_000339"
     CDS             complement(6067..8298)
                     /gene="pflB"
                     /locus_tag="MB219_000339"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCC8426076.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(8575..11025)
                     /gene="parC"
                     /locus_tag="MB219_000340"
     CDS             complement(8575..11025)
                     /gene="parC"
                     /locus_tag="MB219_000340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCC8426077.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(11037..13082)
                     /gene="parE"
                     /locus_tag="MB219_000341"
     CDS             complement(11037..13082)
                     /gene="parE"
                     /locus_tag="MB219_000341"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCC8426078.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(13248..13682)
                     /locus_tag="MB219_000342"
     CDS             complement(13248..13682)
                     /locus_tag="MB219_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCC8426079.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            13809..14447
                     /gene="plsY"
                     /locus_tag="MB219_000343"
     CDS             13809..14447
                     /gene="plsY"
                     /locus_tag="MB219_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCC8426080.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(14579..15454)
                     /locus_tag="MB219_000344"
     CDS             complement(14579..15454)
                     /locus_tag="MB219_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCC8426081.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(15536..16327)
                     /gene="codY"
                     /locus_tag="MB219_000345"
     CDS             complement(15536..16327)
                     /gene="codY"
                     /locus_tag="MB219_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCC8426082.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(16345..17745)
                     /gene="hslU"
                     /locus_tag="MB219_000346"
     CDS             complement(16345..17745)
                     /gene="hslU"
                     /locus_tag="MB219_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCC8426083.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(17759..18307)
                     /gene="hslV"
                     /locus_tag="MB219_000347"
     CDS             complement(17759..18307)
                     /gene="hslV"
                     /locus_tag="MB219_000347"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCC8426084.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(18344..19249)
                     /gene="xerC"
                     /locus_tag="MB219_000348"
     CDS             complement(18344..19249)
                     /gene="xerC"
                     /locus_tag="MB219_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCC8426085.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(19447..20823)
                     /gene="trmFO"
                     /locus_tag="MB219_000349"
     CDS             complement(19447..20823)
                     /gene="trmFO"
                     /locus_tag="MB219_000349"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCC8426086.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(20792..22870)
                     /gene="topA"
                     /locus_tag="MB219_000350"
     CDS             complement(20792..22870)
                     /gene="topA"
                     /locus_tag="MB219_000350"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCC8426087.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(22992..23849)
                     /gene="dprA"
                     /locus_tag="MB219_000351"
     CDS             complement(22992..23849)
                     /gene="dprA"
                     /locus_tag="MB219_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCC8426088.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(23905..24672)
                     /locus_tag="MB219_000352"
     CDS             complement(23905..24672)
                     /locus_tag="MB219_000352"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCC8426089.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(24665..25525)
                     /gene="ylqF"
                     /locus_tag="MB219_000353"
     CDS             complement(24665..25525)
                     /gene="ylqF"
                     /locus_tag="MB219_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCC8426090.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            25785..26183
                     /locus_tag="MB219_000354"
     CDS             25785..26183
                     /locus_tag="MB219_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCC8426091.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         6991 a         5559 c         4524 g         9138 t
ORIGIN      
        1 aaaaagtgga agcttcttcc attagtggta tttgtaccat atgtcgctgt gtttttgagg
       61 aacgaaaaac aatttctttt ccgtaagact tatgccgatt tttttctaca aatcgttccg
      121 tttgtatata agggattttt tcgtcagctt gtccatgcca aaagaacaga ggacgtccag
      181 ctaacttttc cggctgcaat gacaaatcat agctcttaac ccatgaaatc aaatcaaaat
      241 agtcttttgg tacgacataa ttatattccg atgcgcgcct tacgatttct tttccatatt
      301 ccaaaggtgc aggggaaccc atcaaacaca cgcccgctac tatatgagga tgtttagcca
      361 acaaggcgca tgttgtcatt ccccccatcg aaaccccgcc aactgcaagc tgatcatgta
      421 gaagacctct tttttctaaa tgcttgataa gtgtgtcaaa ttcaaacaga tttccgtaga
      481 tactatccca aaatgtatag gatggaatag aagaaaccgg ctgtttgcgc tccccatgat
      541 tcatcgcatc tggcaggatc acacgaatgc cctgctgcgc aatttttctt ccttgcgtca
      601 aaactaactc cttacttgtc tgccatccgt gataatagat gacaacggga atacgttcct
      661 ctcttaattc tttcagaaca acttctaaca ctggaatatg atggacagaa cgtttcaata
      721 taattggatt caattgttac tcacctattt tcattaaatt tcctgatcgc catagcaacc
      781 atgctccaac agttgtctgg aacaggttca gtgtaccaaa taatataaat gcaggactga
      841 atggctcggg aaataagcac agagaacaaa gtcccaccac tcctaatgct agcgaaagga
      901 gtggataaat agaaaaattc aaaaaactgg caacgctacc gaaaatatta ccaataaaca
      961 gtatcaaaaa gaaaaaaacg cttaaaatta aaccatctgt cagcgacatc tgaaaaacaa
     1021 tgttctctac ctcaattggc tgttgaatcg aatagacacc taagtatata ctgagaagga
     1081 gacttagcac gtggctggca caaaacacac gattgaccgt tttcatttta atcacctaac
     1141 ctatctttat aattctatta taatcatttt atcatttttt tgtatgtata aagtttcaat
     1201 ttttcttcac ttacgatttt ttttgatatt gttgttatat aaatgaaagg agttttacaa
     1261 atgattgatc caaaaatgtt ggggatgatc ttcctcatta attttgctta tatcactctg
     1321 aacacgttac gtttcatgtt gacaatgaaa ggctatcgac tgattgctcc acttgtcagt
     1381 atggtcgaga tcacgattta tatcgtcggc ttatcccttg ttttagatcg tttagataat
     1441 ccactgaatt tatttgtcta tgcgttaggt tacgcagtag gtatcagtgt cggaattttg
     1501 attgaagaca aattggcatt aggatacatc atggtcacaa cgattttgcc agcaaatagc
     1561 gcagaagata aaagtttgcc gggtatactg agagaacacg gttatggtgt gacacagact
     1621 tctgcaatgg ggctggaagg tgagcggcta gttctcgaaa tactttctcc tagaaaaagc
     1681 gaacgtgaac tgtatcgcct gatcaaagaa atcgaagagc gtgcttttat catttcttat
     1741 gagccaaaat atatttcagg tggtttctgg actaaaaaag tgagaaaacg acaaggaaaa
     1801 taactcacaa acttaaaaac tgtctaatat ctaaattaaa ttttaaatgt tagacagttt
     1861 ttatttatta ttttgattaa aaaacatcga catatactca tcaatttgtt attatacttt
     1921 atatacttat caatttaatt tggagatatt atggaaattc aacgtagaac cactaatttt
     1981 ttacatttag gctctttttt tgcaactttg acgatcttat tttttctatg ttattcatac
     2041 cttaagacct cacatgcgcc ttctagtgca ttaggctggt tgcttttgga tcattctgat
     2101 atcacagaag ccgaagaaaa tccgtttttt ttgattttgt cttctctttg cggcttactg
     2161 ttttctgttt atttcggtgc ctattttttt catcgacatc ctggtacgtt aaacgaagct
     2221 tcaagcgtat tgattgcatt taaaacgttt tcttttttag caattctttt ttattcgttt
     2281 aatcacaata cgcttttctt ctttatcatg aatggtttag tcgtgatttt atccattttt
     2341 acttatttta ttttttcact catttttagc caattgaaat gtccgctctt ctttcgatta
     2401 gttcctgtca cctgttttat ttacagcttg ttttcctttt ttgtccttgc tttaatgcca
     2461 ctgaccaacc cttcgacgat ccaagtcgca ggaaatctgt tggatatgct cttcatcgtc
     2521 agtctaggtg tatttatgtg gcaaagaaaa aacaaacaaa ataaaaagca ttttgaagcc
     2581 tgactattac gacttcataa atgctttttt agttttaaac acttcaatct aaaagaataa
     2641 cccaagattg gataaattat taatgactcg ccattcgttt cttagtgatt ctggattatc
     2701 aaatggactt cctaatctaa tcagctagtt cttcccataa aatgccatac ttgactgaat
     2761 cataatattg attatttaaa aatctaactt ttcttatcac tacttccttt gtcattccac
     2821 atttctcacc atttttttgc attcctttgt ttccagacca tgtagtgaat ctaataggtg
     2881 gtagataggc aaaatactca aataattctt ttaaccataa aggaagagaa aggctcctcc
     2941 tgcaagagtc agtataattt ttgcttactg ctctcttagg ttttactcac taaaactgtt
     3001 ccatattctt tgatataaga aaatgcatgc ggtataaatt tcttgctcag ctcagaaaag
     3061 atttgtcgtt taagaaaggc catttcctcc attgtcaatg aaggatccaa tgttggtaat
     3121 tcaatctcaa aattgttttt ttcgacaata ggtaattcta atgtgaatga ttcttcttcc
     3181 tttatagtat tcgtcacaag tagagactga tggaaaatac taggccggcg atttttatat
     3241 ttattcttac gtatcctgtc aacgataact tctatttgat caaaaaagta cgattcattt
     3301 acgtctccaa taagttcggc taattctttg aatgccttcg ttacaggaag ctgattcttc
     3361 tgtacaaaaa agtaattagc caaagtaagc gggaagtgtt ctatagagga aagtgtgata
     3421 aaacggtact tctcatttcc tgcagttgtc ctcagcaatt ctgtctctcg gctgtttctt
     3481 actccataaa aatacgcttc tttccgttca tcttcgaaac aatacgccca aataacttct
     3541 cccatcgcta atttttgttc atcaacagag agtctcttca attgttcagc tatggtttca
     3601 gcagccgaag tggcttgttg caagtaatct ttaggatcaa atattggaaa gttcaaatga
     3661 tatcttcgtt cttccctctt gattaatcct gcatcgatca ataaatcaag aaaatgggaa
     3721 aatttatttt gcgaaaattc ttttttcaat tctcttagaa tcgtttcttg ttctcgatgt
     3781 tcatatagat aatcggtcaa tgaagaaaaa agcggatcat cggtcagttt ttgataaaag
     3841 ttttccttat tttttgaata atagttcata tatgttctct tttctatccg ttttatttta
     3901 cttaaaaggc ttttacgtaa aaaggaggaa gaacaagagt cataattgac tgttattctc
     3961 ctccatttct tcggccttta gtatgttcta tgtttctttt tatatcaatc gcttatccat
     4021 tcattttgaa tgcttcatac taccatttga ttagctaaat acagccgtca attgaggtac
     4081 gacttgtttt ttacgtgaaa cgacaccttt aagcaaagca cggttgttgg tcaaagttgt
     4141 ttggaaagct tcttctactt tttcttttcc tttaccagca acaagtaatt ctgaatcact
     4201 gtctaagatg tttgtgacaa ctaatacgaa cagatcgtag ccatttgctt tcatttcatc
     4261 ttccattgct gcttctagct ctgcttgtcg atccatcact tcattaaggt ccactgtatt
     4321 gacttgagcg atacgaacgt tggaactgcc cattgggaaa ctttttgcat ccaaatcaag
     4381 aagaaccgca gctgatttat cacttagatt tgttcctgct ttcaacattt caagtccata
     4441 agtgtttaga tcgacttcag cgattgctgc aagttctttt gcagctgctt catcttcagg
     4501 cgtacatgtt ggtgatttga acaacaatgt atctgaaatg atagctgaga gcatgattcc
     4561 tgcgatttct ttaggaattg ttacttggtt ttctttatat agttttaaaa taatcgtact
     4621 tgtacatcca actggttctg cacgatagta caatggatta gctgtttcaa agtttgcaat
     4681 acgatggtgg tctactacag ctaaaatatt tacttcgctg atatctgaaa tactttgttg
     4741 gaattcatta tgatccacaa gcatgacatc tgcattttct gctttagctg actctactac
     4801 tcgtggtgca gatagctgaa agtggtctaa tgcgtattgt gtttcttcat taggtgtacc
     4861 tagagcaact gcttctgttt ctttacctaa ttcattttgc aagtgtgaaa acgcaatggc
     4921 tgccccgatt gcatctgtat ctggattttg gtgaccaaaa actaaaattt ttgacatgtc
     4981 attcgctcct ttgtgatttc tcctatttaa taatagcaaa aaaacggaaa gaagcaatac
     5041 ttatcttctt tccgatgcga cctatcgcgt taaatatttt gtatactctt ctgtatgaag
     5101 caatcgttta gcattttcga cacgttcttt tgtaggtggt tcgatccctt ctaatggata
     5161 tttcaaacca agtgtttccc acttgtattt tcccatcgta tgataaggaa gaatctctac
     5221 tctgtctaca tttgacaagg tttgaataaa gttgtctagt cggatcaaat attcatcata
     5281 atcacttcgc tcaggaacta gtacatggcg aatccaaacc ggtttgttga tttttgacaa
     5341 atattgagcc atttctaaaa tattttcatt gtcccaatgc gttagttttt tatgctcttc
     5401 attatcgatg tgtttgatgt caaataacag taaatctgta tacttcatca attcttgaaa
     5461 acgtgaaaag aaaggttctt catacgtaaa aggttttccg catgtatcca atgtagtatg
     5521 catcttccgc tcttttgctt ttttaaatag ttcgatcaaa aaatcgatct gcaatagagg
     5581 ttctccacca ctaacagtaa taccaccttt atctccccag taaggtctga agcgttctgc
     5641 tttatctaaa agttcatctg ctgtgtattc tttccctccg ccaatattcc aagtatctgg
     5701 attgtgacaa aattggcaac gcatgcggca tccttgcata aacacgacaa agcgcagtcc
     5761 aggtccgtct actgaaccga aagtttcgat agaatgaaca tagccgattg ttttttcttc
     5821 catttaagtt cacatctttt ctttgatttc attttaagta caagttagct gtcgaagcga
     5881 agctttctga acaaaatttt tcgcaattgc cgcttcgtta tgtgtaaaat ttacagggac
     5941 ggaacataca agtcctgccc ctgtaaatcg tttctttcaa ataagcatac tgattattcg
     6001 attttgatgc gatggctatt caacgtcctt tcgacgctaa gtcgaaaaac ttttttcacg
     6061 aactttttac attctgtcgt gtgaagttct catcaagaca tctaattgtt gttctcttgt
     6121 caagtcacgg aatttaactg cataaccaga tacccggatc gtcaagttcg gatatttcat
     6181 tggattttcc attgcatcac gcaataaatc attactgaat acattgacat tcaaatgata
     6241 agctcctttt gagaagtatc catctaagac attacgcaag ttttcgattc ttgtatcttg
     6301 ttcttttcca agacctgaag gattgattgt ttgggtattt gaaattccat ccaatgcaca
     6361 gccgtaattc attttagctg ttgagttcag gctagctaat aacccacttt gttccgcatt
     6421 gtaacttgga tttgctcctg gcgcaagtgg ttttccagct tcacggccat caggagtatt
     6481 tcctgtattt ttaccgtaaa caacatttga agtaatcgtc aataatgaag ttgttggttt
     6541 tgagttgcgg taagttttat gacgtttgat ttgtcccatg aagtattcca agatccatga
     6601 aactaattca tctgcacggt catcatcatt tccgtattta gggaagtcgc catccacttc
     6661 aaaatcaact gctattcctt tttcattacg gattgctttt acttttgcat atttgatcgc
     6721 tgacaagctg tctgccgcat gagagatccc agcaatacct gtagcaaatg tgcgttgcaa
     6781 gtcagaatcc atcaaagcca gctgagctgc ttcataagaa tatttatcat gcatataatg
     6841 aatgatgttc aacgtattga catatagttc tgctaaccaa tccatcatat tcatgaagtt
     6901 atgtttcact tcttcaaagt ctagtatttc tgatgtgatt ggacgatatt ctggtccaac
     6961 ttgggcgcca gtcacttcat cgactccgcc attgatggca tataatagtg ttttagccaa
     7021 gttagcacga gcgccaaaga attgcatgtc ttttccgaca actgttcctg atacacagca
     7081 agcaatcgca caatcatcag aaccccattg ttcacgaagc tgattatcat tttcgaattg
     7141 aatcgaagaa ctttcgatag cgattctgct agcatatgtt ctgaatcctt ctggaaggtg
     7201 ttcagaatac aaaacagtca agtttggttc tggtgaaggt cccatatttg ttaatgtatg
     7261 aagcagacgg aaatcattct ttgtaactaa tgaacgacca tctaatccca tacctgcaat
     7321 ggataaagta gcccagatag ggaatcctga gaataattgg ttatattcag gtgtacgcgc
     7381 gaatttgacc atacgtaatt tcattactaa gtggtcgatc aattcttgtg cttctttttc
     7441 tgtgattact ccattatcca aatcacgttg gatatagata tcaaggaatg ctgagatacg
     7501 tccgattgac atcgcagcac cattttgtga tttgattgct gctaaatatc cgaagtatag
     7561 ccattggaca gcttcttttg catttgcagc tggtttagaa atatcatagc catatttcgc
     7621 agccatttct ttcatttgcg ctaatgcacg atattgttca gtgatttctt cacgtaaacg
     7681 aatcacttct tcactcattg tatgataacc agtcatatca tgatcttttt tcttttgttc
     7741 catcaaatag tcgattccat acaatgccac acgacgatag tcaccgatga tacgtccgcg
     7801 accgtaagcg tctggaagcc ctgtaatgat tttgttttta cgagcagcac gcatttcagc
     7861 agtataagcg tcaaatacac cttgattatg tgttttgcgc cagtcagtga aaatatgtgt
     7921 caattcatcg tttggtgtat agccattgct ttttagcgca ttgttagcca tattgattcc
     7981 gccgaacggc atgaatgctt gtttcaaagg tttgtctgtc tgtagaccga cgattttttc
     8041 caaatcttta tccaaatagc ctgcatcgtg tgaagtaata gttgatacga tttctgtgtc
     8101 catatcatag acaccgttac gctcgaattg gatatcattt aacttttgca attcatccca
     8161 taatttttct gttgcttctg ttgggccttc caaaaagctg tcatcaccgc gatattcagt
     8221 gtagttattt tgaataaagt ctctagtgtc tacgccttct ttccatttgg tacctttaaa
     8281 tccattccat tgctccattg tatggacctc ctaaatttct gttttatgta acagttttta
     8341 cgtgattagt ataacacagc accctatctt tgcaagggtt ttcttgtttt ttatttaaca
     8401 atttttatgt tttatttaca tatatttaaa tcaacgttga cataccaaca tttaagcatt
     8461 gtgaacactt tcatcttttt ttgcaaacgt ataaggattt attaaaaatg aatgaaaaag
     8521 agacgccacc tgtattaata cagatggcgt ctcttcatta aaatactaca acagttacga
     8581 ctcgatgaaa atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc
     8641 catcgcaaat gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt
     8701 taactgctgc cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata
     8761 gataatacgg tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg
     8821 tgaagtttgg ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat
     8881 tacgatcggc gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc
     8941 tttcaaattc atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa
     9001 aggatagcgc aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc
     9061 ttctttcaga tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag
     9121 cggacggctc ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa
     9181 tccattcttg ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac
     9241 agctaaaatc gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc
     9301 atctttccat ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt
     9361 tgtgatcaga agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc
     9421 ttctcgcatc ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt
     9481 cacatagcct tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt
     9541 atcaattttg atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt
     9601 ttttttcact tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag
     9661 gattttattc aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt
     9721 gatatccgtg ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc
     9781 tgtaaattga aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg
     9841 aatcgttgcg attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg
     9901 ctgtcttttt tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg
     9961 atgctcgata taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc
    10021 gatagcgacc atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt
    10081 caaaattcct tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct
    10141 atcactttca tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc
    10201 catttttttc accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg
    10261 ctgtttatta cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg
    10321 tcctgtttca taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa
    10381 gtcaggtcca ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat
    10441 atagattgtg ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc
    10501 agcagatata cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt
    10561 tggttctttt tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc
    10621 tgctagcatc tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg
    10681 tggatcaccg tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt
    10741 ccagtcttgg ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata
    10801 attccccatg atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt
    10861 gccatctttg ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat
    10921 atcaggcagt gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat
    10981 cacttcttct aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat
    11041 tctacttcaa ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca
    11101 ttagaaactg aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct
    11161 tcgagcgtaa attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc
    11221 atcagtgtgg ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac
    11281 gtacgggaag tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt
    11341 cctttatatc gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg
    11401 tcatctgtcc acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat
    11461 aaaggcggta acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa
    11521 aagaaagtaa gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg
    11581 attactttgt cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc
    11641 gtatagatca tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg
    11701 gtattgatga cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt
    11761 ttagcagatc cgccagcaga atccccttcg actaaataca attcattttt cttagggttt
    11821 cgggattgtg cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc
    11881 ccatttcggc tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt
    11941 gctttacgga ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt
    12001 tcactgatga cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt
    12061 tgcccttcaa attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct
    12121 tctctgaaat cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc
    12181 gcatattcat tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca
    12241 tcttttgtcc gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat
    12301 tgataagcga cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc
    12361 aatgtatctt tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga
    12421 aaaatttcct tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt
    12481 agaagaaagg cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta
    12541 ctgaatattg tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt
    12601 cctatctttt tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct
    12661 acgccatctc taacgatcgt tacaattagc catttcgata aggcatttac cacactggct
    12721 cctacaccgt gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca
    12781 tgcagcacag taaaaatcac ttcaactgtt ggtattccgg aagcatgcat tcctacaggc
    12841 atcccacgtc cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc
    12901 tcactgccat aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa
    12961 tgatgcaagc ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc
    13021 tctaatcctt ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc
    13081 aaggcaaacg ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag
    13141 aaaaggtcgt gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc
    13201 tttttcgaca ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt
    13261 tctttcaact taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct
    13321 gcttttctta gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact
    13381 ttagcatcag ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat
    13441 acatctacga tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct
    13501 agaatttctt caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc
    13561 aatacctgtg caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca
    13621 atattttttg cttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac
    13681 atttctgcca ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt
    13741 ttcaattgtt cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag
    13801 ggtaaaaaat gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt
    13861 ccggtgtctg gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta
    13921 atactggaac gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc
    13981 ttatggatat tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg
    14041 ggattaaccc tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg
    14101 ctgggttcaa aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc
    14161 ctgtattttt tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg
    14221 tgagtttgag cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt
    14281 atgtagctcc tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag
    14341 tttcttcctt tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg
    14401 aaagtcgaat cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc
    14461 caaagagata aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat
    14521 agctttttta cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt
    14581 acttcactgt gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc
    14641 ctactttttc catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt
    14701 cgatacaaac gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat
    14761 ctttatagct taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta
    14821 gaccccttgt ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat
    14881 tcgtatttgt ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta
    14941 ggcgtaatct tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt
    15001 cttcaagagg tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac
    15061 cggtatttgc cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca
    15121 ctaattcaaa atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac
    15181 tagccatttc ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt
    15241 gccccatttg atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa
    15301 aagggaataa aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact
    15361 ctaaaccatg ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg
    15421 cgatcagctg gtcattttga ataataacag tcattgccat tcctccaact tcgacttact
    15481 ttttaaaatg ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat
    15541 aactgtgttt tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct
    15601 tcatgcctaa cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga
    15661 cgatcacaga acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt
    15721 catctccatc caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg
    15781 tgttgattgc catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact
    15841 tctgataaag gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt
    15901 catctgcaaa cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc
    15961 caaagatcgg aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa
    16021 atgcagtaat atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg
    16081 tatcagtata agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt
    16141 tcacatcgtg acgttcatta taacctagta atactccgtc acttcctatg atataggtgt
    16201 tgctgtcaag aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca
    16261 tgtcaaaagt attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt
    16321 ctgtcattgt cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc
    16381 aattgagcca agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat
    16441 atctggtgct tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg
    16501 tgcgccgata ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat
    16561 tgcttctttt gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt
    16621 tactaaagca ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc
    16681 taattctacc cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga
    16741 caaatggaaa gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt
    16801 tgtgtttact tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga
    16861 tacttctcca gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat
    16921 tccactttct tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt
    16981 tgctgattct tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac
    17041 ttttttcttc ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa
    17101 tccattgttc atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg
    17161 actatccagt aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc
    17221 ttctgtcact tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat
    17281 catttgttca aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat
    17341 ttttaccaaa cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc
    17401 tttttctacg atttggatcg cattttctac taaatcccga accattgatt ctacatctct
    17461 tccgacataa ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac
    17521 gatttttgcc aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag
    17581 catatttttt ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg
    17641 gttacgcaaa gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc
    17701 taattcactt acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt
    17761 acagttcttc tacgataata ttatgattag taaagataca gatatctgcg gcaatattta
    17821 acgcattttc tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc
    17881 gggctgcagc caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg
    17941 tgataacttc tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca
    18001 tcgcttcaag tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg
    18061 cgcgttgtag attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa
    18121 acgcatctgc aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta
    18181 ctttctttgc agtgcctttc atgacaacag attcacccat tgttacttgg ccatctccag
    18241 ccatagcaaa tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt
    18301 cgaccatgtt tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga
    18361 aagaacgata gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg
    18421 ataaattggc atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata
    18481 aatgagtagc aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt
    18541 ttttgatcat ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat
    18601 gattcacaaa aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt
    18661 ccctgaaata ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt
    18721 tgttcccttt accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca
    18781 aactgctaca ctcacttaaa cggatgcctg acccgtaaag gacttccagt aaagcgcggt
    18841 tcctcaaatc caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt
    18901 cataaaaaaa acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat
    18961 tttcttttat cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct
    19021 tgcgactgat agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga
    19081 cacgaaccgt aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat
    19141 gtttgaaatg gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt
    19201 cgacaatcaa ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt
    19261 ccttttgtcc cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga
    19321 atttaaccaa tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag
    19381 aaattcttaa gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga
    19441 gtttccttac tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc
    19501 ttttaaagct cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg
    19561 cagttcagga aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg
    19621 agtaatgtag taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat
    19681 ttcttttgct aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac
    19741 atagccttct acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg
    19801 gtaagtcggt ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata
    19861 gcgaacgaat tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc
    19921 ccatttcaag tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg
    19981 acgcagctgg ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg
    20041 tttcataggt ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat
    20101 acatccttca aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat
    20161 tagtgcctca tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc
    20221 ttctcctttg tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc
    20281 tacgattggt gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat
    20341 gctttctgct aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg
    20401 tatttcagta atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt
    20461 aattgtttca gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt
    20521 ttcatctgcc gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc
    20581 tactgcatta gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt
    20641 acttgtgtga tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac
    20701 tcctgcttgt gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt
    20761 taccattttc aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc
    20821 catttacaca gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac
    20881 attttggaca atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac
    20941 gggaacaacc atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat
    21001 gacatactgg gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag
    21061 gaaaattact gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc
    21121 cacagacatc acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt
    21181 cttccgcatt cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc
    21241 acgcttccgt gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga
    21301 tatcgacgat ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag
    21361 tcggctcaaa tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg
    21421 ttggtgcata agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag
    21481 cctcactaaa acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt
    21541 ggactacatc gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat
    21601 ctctaccttc cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa
    21661 tgaagatgac accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca
    21721 tctgactagc cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta
    21781 aatattgttt gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg
    21841 catcttgtgc gccttgagca tttttgatct ttcggtgatc atgtctagaa aattcttttc
    21901 catacgtatc ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag
    21961 aatcagtacg catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt
    22021 catataattg ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag
    22081 cttcttgttg caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct
    22141 tttctacttt tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac
    22201 tttgtgcatc tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt
    22261 tcttgcgagc ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc
    22321 ggatctcttt ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac
    22381 ttaacccttt cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt
    22441 ctaaggtacg acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct
    22501 taaaagctgc tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt
    22561 tcagatccaa cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg
    22621 ggtcacttgc caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga
    22681 catcgccttt tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac
    22741 ccattttact tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc
    22801 gtccaagata tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt
    22861 tatacgccat tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat
    22921 tcatacaaat cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga
    22981 aaagtatggt gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact
    23041 gcaccgtctt ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga
    23101 acagaaaaaa ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt
    23161 tttttcttgg cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc
    23221 attggaaaac aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga
    23281 tcatttgcaa tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct
    23341 gttccaatga ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta
    23401 tctatccctt tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt
    23461 aaataataaa ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac
    23521 atgttacaag aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc
    23581 aagtgaagca gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat
    23641 tggattttgc cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt
    23701 ttggttattt tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc
    23761 atcatccagt tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt
    23821 ttcaataaat tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat
    23881 aagatacgta aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt
    23941 acgatggatt ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata
    24001 acctgcatta tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg
    24061 gtctcgatat actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc
    24121 tttgattatt ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata
    24181 gcttggtaca atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata
    24241 aatattcaca tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt
    24301 ttcttgaact tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt
    24361 cagccctaat atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc
    24421 taatggtcct cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc
    24481 ttgcttttca aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa
    24541 tcgtcgttca aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga
    24601 ttctagtgta ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat
    24661 ggattcagtc atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa
    24721 gttttccttg acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat
    24781 acccacgttt tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga
    24841 ttgataaacc ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc
    24901 cgtaaatcgc taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta
    24961 gttttttgcc gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca
    25021 attccaactc attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc
    25081 ctgtctgtgc aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg
    25141 gaatacctaa gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg
    25201 cccgtttttc tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt
    25261 cttgtgccgt gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct
    25321 ttgtctgttg tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct
    25381 gaatgatctg atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt
    25441 caaatacgat gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat
    25501 gtcccggaaa ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc
    25561 cggctttact tgtttttttc tttttcaata taaattcact tttttattat aaagaaattc
    25621 catctattag ttaagtgaaa gtgaaccagt cctcattcta tcacatcttc acttttttga
    25681 tgatgcaaat ttttattttg gtttctatat atgaatctaa atttttgaca gcgtttccta
    25741 aataagagat gatagacgaa caaaacagaa aggagacgaa acaaatgaat agtatggttt
    25801 ttgttaattt tcctgttgct gatgtcaatc gttccgttgc tttttatgaa aagctgggtt
    25861 ttaaaaaaaa tgaagaattt tctactgaag aagctggttc aatggtatgg gatgacactt
    25921 tttggataat gcttctctct catgattttt ataaaaaatt tataaaagat aaagaaatag
    25981 ctgataataa aaaaacaagt ggtgctttga tttcgtttag tgttgaaaat gcggatgaag
    26041 taaaaaaatt tgctcaaatt gcaaaagaaa atggtggtac tttccacgaa gtagatatgg
    26101 gaatgcctga agaagaaatg ttttcattag aagtccaaga tctagacgga aatacattag
    26161 aacccgtatg gatgaagatg taagcagata actaatctga cgttttttat aa
//
LOCUS       DAHEYX010000012        25107 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHEYX010000012 DAHEYX010000000
VERSION     DAHEYX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.012"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            118..555
                     /gene="zapA"
                     /locus_tag="MB219_000355"
     CDS             118..555
                     /gene="zapA"
                     /locus_tag="MB219_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCC8426092.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            613..1161
                     /locus_tag="MB219_000356"
     CDS             613..1161
                     /locus_tag="MB219_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCC8426093.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            1276..3636
                     /locus_tag="MB219_000357"
     CDS             1276..3636
                     /locus_tag="MB219_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCC8426094.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            3723..4037
                     /gene="trxA"
                     /locus_tag="MB219_000358"
     CDS             3723..4037
                     /gene="trxA"
                     /locus_tag="MB219_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCC8426095.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            4219..5997
                     /gene="uvrC"
                     /locus_tag="MB219_000359"
     CDS             4219..5997
                     /gene="uvrC"
                     /locus_tag="MB219_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCC8426096.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            6142..7041
                     /locus_tag="MB219_000360"
     CDS             6142..7041
                     /locus_tag="MB219_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426097.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            7034..8296
                     /locus_tag="MB219_000361"
     CDS             7034..8296
                     /locus_tag="MB219_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426098.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            8457..10355
                     /locus_tag="MB219_000362"
     CDS             8457..10355
                     /locus_tag="MB219_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCC8426099.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(10685..11404)
                     /locus_tag="MB219_000363"
     CDS             complement(10685..11404)
                     /locus_tag="MB219_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCC8426100.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            11741..12073
                     /locus_tag="MB219_000364"
     CDS             11741..12073
                     /locus_tag="MB219_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426101.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            12060..14063
                     /locus_tag="MB219_000365"
     CDS             12060..14063
                     /locus_tag="MB219_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCC8426102.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            14082..14552
                     /locus_tag="MB219_000366"
     CDS             14082..14552
                     /locus_tag="MB219_000366"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCC8426103.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            14573..15157
                     /locus_tag="MB219_000367"
     CDS             14573..15157
                     /locus_tag="MB219_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCC8426104.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            15172..16158
                     /locus_tag="MB219_000368"
     CDS             15172..16158
                     /locus_tag="MB219_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCC8426105.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            16148..16459
                     /locus_tag="MB219_000369"
     CDS             16148..16459
                     /locus_tag="MB219_000369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCC8426106.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            16481..18262
                     /locus_tag="MB219_000370"
     CDS             16481..18262
                     /locus_tag="MB219_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCC8426107.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            18255..19631
                     /locus_tag="MB219_000371"
     CDS             18255..19631
                     /locus_tag="MB219_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCC8426108.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            19638..20273
                     /locus_tag="MB219_000372"
     CDS             19638..20273
                     /locus_tag="MB219_000372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCC8426109.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            20303..21658
                     /locus_tag="MB219_000373"
     CDS             20303..21658
                     /locus_tag="MB219_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCC8426110.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(21850..23244)
                     /locus_tag="MB219_000374"
     CDS             complement(21850..23244)
                     /locus_tag="MB219_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCC8426111.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            23336..24307
                     /locus_tag="MB219_000375"
     CDS             23336..24307
                     /locus_tag="MB219_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCC8426112.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            24304..25086
                     /locus_tag="MB219_000376"
     CDS             24304..25086
                     /locus_tag="MB219_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCC8426113.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
BASE COUNT         8312 a         4225 c         5471 g         7099 t
ORIGIN      
        1 tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag
       61 aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg
      121 gcgcacgaaa aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga
      181 cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa
      241 ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat
      301 gcgctttcgg atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca
      361 gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa
      421 gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt
      481 aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa
      541 aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga
      601 aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct
      661 ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt
      721 attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata cattccatac
      781 ccagctgtca ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta
      841 gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt
      901 acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta
      961 gactggattg caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt
     1021 ttgcagttac ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt
     1081 ttcgctcgtt ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg
     1141 gtaacgaata ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga
     1201 gtagtttatt taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa
     1261 agataaggtg ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa
     1321 cagatggtca gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta
     1381 ccagtgagtg agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa
     1441 gtgcaacgtc tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg
     1501 aaacggattg agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta
     1561 ttgtctacga caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa
     1621 tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt
     1681 ttaaaggaag caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag
     1741 agcataagac agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc
     1801 gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc
     1861 tacgttatcc cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa
     1921 agtgcttcag gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt
     1981 ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct
     2041 gctgaacttg ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg
     2101 gatctgatga atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc
     2161 agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa
     2221 gtagtttcta atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct
     2281 aacacaggtg ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa
     2341 gcaggattac cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt
     2401 gcggatatag gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg
     2461 acgaacactg tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag
     2521 ctcggtgcag gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat
     2581 cttggtaaaa agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat
     2641 ggatataatc gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt
     2701 ccaacgtatc gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc
     2761 cgattaggtc ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc
     2821 caagatttaa acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa
     2881 tatctggaaa tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa
     2941 gcttacagct atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca
     3001 aatgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa
     3061 atgcaacagc agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc
     3121 caattagcaa atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg
     3181 aaagaacaaa aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga
     3241 gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg
     3301 aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt
     3361 atccatgctg tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga
     3421 aaacgatacg aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct
     3481 ggttatcctc aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt
     3541 actgattatc ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa
     3601 ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg
     3661 ttcgctatcc aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg
     3721 atatgtcaca agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat
     3781 tgattgattt ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc
     3841 agttggaaca agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa
     3901 atccagaaac tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag
     3961 atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga
     4021 tcgatcaata tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt
     4081 tccgtcctcg gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat
     4141 aattacgttg tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa
     4201 tatgaagggg agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat
     4261 cagcccggct gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc
     4321 aaaatattaa aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa
     4381 cggcttgtta gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca
     4441 ttgctcttag agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag
     4501 gatgataaaa cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt
     4561 actcgtaagg tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg
     4621 gcgaatgaga caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt
     4681 cagacgaagg agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc
     4741 gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga
     4801 ggacacgaaa aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg
     4861 gagtttgaaa aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact
     4921 cgccaaaaaa tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat
     4981 aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat
     5041 gtttcaatgt ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag
     5101 ttttatcagg aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac
     5161 aagcccagtg tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag
     5221 aaaaaattag ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat
     5281 ctgattgcca gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg
     5341 aatatcccga ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat
     5401 cctgtggctg cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa
     5461 tataaaataa aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat
     5521 cgcagatatt cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat
     5581 ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat
     5641 attcctattg ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt
     5701 acagatttgg ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga
     5761 atccaagatg aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac
     5821 agttttgctt cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg
     5881 cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa
     5941 atcggactgc cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga
     6001 aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt
     6061 ttacgttttg ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt
     6121 tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac
     6181 tacacagctg tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt
     6241 ggccaaaacg gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa
     6301 gaccaaggag aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc
     6361 ggttatcttc cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt
     6421 tattttgcag aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg
     6481 atggaaaaat ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga
     6541 aatcagcaaa aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg
     6601 gatgagccat tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg
     6661 gaattgaaaa aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa
     6721 aaaatctgcg atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta
     6781 catgagattc gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa
     6841 caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta
     6901 gagattacct tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc
     6961 ggctacattc ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa
     7021 gcaggtgaaa tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt
     7081 taagtcgatt tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta
     7141 tgtagcagga aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga
     7201 taatgagcaa atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc
     7261 agttgattct gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt
     7321 agtagtctca actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg
     7381 acaaagtaca caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc
     7441 gagtcaatta ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac
     7501 tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac
     7561 agtccaatac gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta
     7621 tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat
     7681 cctgtccagt acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt
     7741 tgcattaacg caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa
     7801 agatatggaa atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc
     7861 attcttgtgg tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc
     7921 agctttatgt ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt
     7981 gacttatcta tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa
     8041 caatatcgtt attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat
     8101 tcgcttagca aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt
     8161 agccgtaaca ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt
     8221 ttatagcgaa ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga
     8281 acgaaaaaaa gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc
     8341 agcttctttt tgtatagacc tattttattt attttactat attggatata tattcattga
     8401 aaatagtgta taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg
     8461 accgttattt agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa
     8521 tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata
     8581 tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag
     8641 aaaagatcaa agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta
     8701 tttttacggc ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat
     8761 cgcagctgat ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt
     8821 caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata
     8881 tcgatgaccg tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt
     8941 tgatgaagga agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca
     9001 tcgatcattt gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga
     9061 tggatcgaat catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt
     9121 ggattggtgc gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac
     9181 gctataatta tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt
     9241 cttttgctca tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc
     9301 tatccgaaag agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat
     9361 tcaaatcaga acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat
     9421 tgctggaaaa aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt
     9481 tgaaaggaac ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg
     9541 aacaaaaagt gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac
     9601 atatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt
     9661 ttcatggctg tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat
     9721 cttatcgcgg aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag
     9781 atctgactgc aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat
     9841 tttcgccttt attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc
     9901 ctcatacgca cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca
     9961 gtgaaaaaat tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc
    10021 acacgccagt aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg
    10081 tcattgacgg aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac
    10141 tattatgcaa ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac
    10201 aacaatttgc aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac
    10261 cacgcaaact caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc
    10321 tcaaaaggct gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc
    10381 atagactgga gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt
    10441 cttctgccaa ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt
    10501 gaaattgctc cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg
    10561 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt
    10621 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag
    10681 tctattaaaa ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt
    10741 gggtaagttc cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa
    10801 ccgcttcaac gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt
    10861 tgactgcctg atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca
    10921 aactgtctga aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt
    10981 catgttttac tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa
    11041 cgacattcaa tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg
    11101 cttccaagat cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg
    11161 gatgaggatc ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt
    11221 tgtcgatttg tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag
    11281 gcaatccttg agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat
    11341 ttccttggaa agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt
    11401 tcatttgtat tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta
    11461 tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt
    11521 tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa
    11581 gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg
    11641 acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg
    11701 aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc
    11761 tttggaacaa atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca
    11821 gcagctgaag atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta
    11881 tcgacaacaa ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga
    11941 aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca
    12001 atatcaaaag catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta
    12061 tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga
    12121 ttttacaagc agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa
    12181 caaacaacca gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa
    12241 aattaagcgc tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac
    12301 accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag
    12361 atttggaagc caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt
    12421 taaaagaaaa gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat
    12481 ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca
    12541 tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta
    12601 agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt
    12661 atgttgcttt aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca
    12721 ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc
    12781 aattatccat tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg
    12841 gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa
    12901 aaataaaaga acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg
    12961 cagaagaaaa acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat
    13021 acgtcagttt cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga
    13081 aaaaccatcc gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat
    13141 atgaagaagt agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga
    13201 tggtcgcaga tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac
    13261 tacttgttct accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac
    13321 catcgatcat ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac
    13381 tgtttggcat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt
    13441 tgattttatc cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa
    13501 aggaaaatat caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag
    13561 ggatcctaat tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat
    13621 ttgtttacct tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga
    13681 ttatccaaac gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac
    13741 tgaccggcta tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag
    13801 gcggaagtat tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat
    13861 tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc
    13921 taagtgccta tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata
    13981 caggaggcgg acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc
    14041 acaagaaaaa aaacaataaa taattggagg atttttatat aatgatggat tacttaatta
    14101 gtcaaaatgg tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag
    14161 ggatcggttc agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa
    14221 gtcaacccga aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt
    14281 tgtatggatt cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg
    14341 tacaaggact gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag
    14401 gtattgccca aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac
    14461 atgctacaaa aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg
    14521 ttatttcctt cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc
    14581 cattgaaaaa atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga
    14641 acaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac
    14701 agaacatcaa aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct
    14761 aaggaatcgc cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt
    14821 aagacgattg tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat
    14881 ccagtttatc aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc
    14941 tggagaaaaa tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact
    15001 tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa
    15061 cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac
    15121 gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac
    15181 cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt
    15241 gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt
    15301 tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag
    15361 cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc
    15421 tatacgatgc gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat
    15481 caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat
    15541 gccattcata cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa
    15601 gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga
    15661 cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca
    15721 gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag
    15781 aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa
    15841 gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct attagagaca
    15901 gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt
    15961 gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc
    16021 ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt
    16081 ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga
    16141 caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa
    16201 attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga
    16261 agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag
    16321 cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa
    16381 tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt
    16441 aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat
    16501 aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat
    16561 gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc
    16621 gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac
    16681 tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat
    16741 ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt
    16801 tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg
    16861 agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca
    16921 taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt
    16981 cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat
    17041 gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc
    17101 acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc
    17161 agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa
    17221 atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac
    17281 ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga
    17341 acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat
    17401 ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat
    17461 ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga
    17521 aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga
    17581 acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat
    17641 cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg
    17701 ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc
    17761 gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca
    17821 tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga
    17881 acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg
    17941 tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga
    18001 tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac
    18061 ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga
    18121 cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt
    18181 agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga
    18241 aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga
    18301 tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa
    18361 atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa
    18421 tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc
    18481 ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac
    18541 cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa
    18601 tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg
    18661 atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc
    18721 ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg
    18781 atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg
    18841 aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg
    18901 accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg
    18961 cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg
    19021 cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt
    19081 atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag
    19141 gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc
    19201 ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga
    19261 gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa
    19321 ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct
    19381 atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg
    19441 atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag
    19501 gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga
    19561 tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag
    19621 aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc
    19681 gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag
    19741 atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa
    19801 tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg
    19861 aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag
    19921 acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga
    19981 cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg
    20041 atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc
    20101 aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga
    20161 cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc
    20221 gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat
    20281 ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc
    20341 ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact
    20401 taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac
    20461 tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa
    20521 cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat
    20581 ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat
    20641 attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat
    20701 cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt
    20761 gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt
    20821 ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta
    20881 tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg
    20941 tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac
    21001 cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg
    21061 ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt
    21121 gacacctaga acggctggat attattcagt tgattacctc catatgagcc atgcaggtct
    21181 gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt
    21241 gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac
    21301 gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt
    21361 attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac
    21421 gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac
    21481 agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat
    21541 agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa
    21601 agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt
    21661 tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg
    21721 cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg
    21781 aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt
    21841 atcaataggt tataattcat atttttttat tttttctttt acgatactta gtccagaagc
    21901 taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg
    21961 tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat
    22021 tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt
    22081 tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg taagacttaa
    22141 tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac
    22201 atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac
    22261 atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc
    22321 agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg
    22381 atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc
    22441 aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt
    22501 acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat
    22561 atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg
    22621 agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga
    22681 gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga
    22741 ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa
    22801 atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt
    22861 tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa
    22921 atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg
    22981 agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt
    23041 ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga
    23101 gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat
    23161 caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta
    23221 attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac catacaattt
    23281 taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa
    23341 aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat
    23401 gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga
    23461 aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga
    23521 gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt
    23581 accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt
    23641 gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga
    23701 ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt
    23761 gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat
    23821 tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac
    23881 gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt
    23941 gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa
    24001 tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc
    24061 tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc
    24121 catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc
    24181 tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct
    24241 agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt
    24301 aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat
    24361 ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc
    24421 attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt
    24481 agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca
    24541 tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc
    24601 ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg
    24661 ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa
    24721 gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg
    24781 cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca
    24841 gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa
    24901 gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat
    24961 aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta
    25021 aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt
    25081 atttgatttc tttcaaatga attaaaa
//
LOCUS       DAHEYX010000013        25057 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHEYX010000013 DAHEYX010000000
VERSION     DAHEYX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.013"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            104..292
                     /locus_tag="MB219_000377"
     CDS             104..292
                     /locus_tag="MB219_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCC8426114.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(350..703)
                     /locus_tag="MB219_000378"
     CDS             complement(350..703)
                     /locus_tag="MB219_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426115.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1234..1656
                     /locus_tag="MB219_000379"
     CDS             1234..1656
                     /locus_tag="MB219_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCC8426116.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1653..1883
                     /locus_tag="MB219_000380"
     CDS             1653..1883
                     /locus_tag="MB219_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426117.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2381..2581
                     /locus_tag="MB219_000381"
     CDS             2381..2581
                     /locus_tag="MB219_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCC8426118.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2608..3801
                     /locus_tag="MB219_000382"
     CDS             2608..3801
                     /locus_tag="MB219_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCC8426119.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3869..5434)
                     /gene="guaA"
                     /locus_tag="MB219_000383"
     CDS             complement(3869..5434)
                     /gene="guaA"
                     /locus_tag="MB219_000383"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCC8426120.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5493..6416)
                     /locus_tag="MB219_000384"
     CDS             complement(5493..6416)
                     /locus_tag="MB219_000384"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCC8426121.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6612..7217
                     /locus_tag="MB219_000385"
     CDS             6612..7217
                     /locus_tag="MB219_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCC8426122.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7525..8541
                     /locus_tag="MB219_000386"
     CDS             7525..8541
                     /locus_tag="MB219_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCC8426123.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8682..9983
                     /locus_tag="MB219_000387"
     CDS             8682..9983
                     /locus_tag="MB219_000387"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCC8426124.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10029..10691
                     /gene="deoC"
                     /locus_tag="MB219_000388"
     CDS             10029..10691
                     /gene="deoC"
                     /locus_tag="MB219_000388"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCC8426125.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10712..11110
                     /locus_tag="MB219_000389"
     CDS             10712..11110
                     /locus_tag="MB219_000389"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCC8426126.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11176..12258
                     /locus_tag="MB219_000390"
     CDS             11176..12258
                     /locus_tag="MB219_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8426127.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12470..14032
                     /locus_tag="MB219_000391"
     CDS             12470..14032
                     /locus_tag="MB219_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426128.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14025..15158
                     /locus_tag="MB219_000392"
     CDS             14025..15158
                     /locus_tag="MB219_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426129.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15158..16114
                     /locus_tag="MB219_000393"
     CDS             15158..16114
                     /locus_tag="MB219_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426130.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16213..17379
                     /gene="deoB"
                     /locus_tag="MB219_000394"
     CDS             16213..17379
                     /gene="deoB"
                     /locus_tag="MB219_000394"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCC8426131.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17403..18221
                     /locus_tag="MB219_000395"
     CDS             17403..18221
                     /locus_tag="MB219_000395"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCC8426132.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18238..18945
                     /gene="deoD"
                     /locus_tag="MB219_000396"
     CDS             18238..18945
                     /gene="deoD"
                     /locus_tag="MB219_000396"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCC8426133.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19243..19929)
                     /gene="gpmA"
                     /locus_tag="MB219_000397"
     CDS             complement(19243..19929)
                     /gene="gpmA"
                     /locus_tag="MB219_000397"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCC8426134.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20101..20682)
                     /locus_tag="MB219_000398"
     CDS             complement(20101..20682)
                     /locus_tag="MB219_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCC8426135.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20815..21498)
                     /gene="rpiA"
                     /locus_tag="MB219_000399"
     CDS             complement(20815..21498)
                     /gene="rpiA"
                     /locus_tag="MB219_000399"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCC8426136.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21817..22230
                     /gene="rpsL"
                     /locus_tag="MB219_000400"
     CDS             21817..22230
                     /gene="rpsL"
                     /locus_tag="MB219_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCC8426137.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22314..22784
                     /gene="rpsG"
                     /locus_tag="MB219_000401"
     CDS             22314..22784
                     /gene="rpsG"
                     /locus_tag="MB219_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCC8426138.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22878..24965
                     /gene="fusA"
                     /locus_tag="MB219_000402"
     CDS             22878..24965
                     /gene="fusA"
                     /locus_tag="MB219_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCC8426139.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
BASE COUNT         7882 a         4605 c         5316 g         7254 t
ORIGIN      
        1 acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt
       61 ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg
      121 gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact
      181 taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa
      241 aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa
      301 atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa
      361 tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg
      421 taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg
      481 taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt
      541 gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa
      601 tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta
      661 aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata
      721 cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg
      781 agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg
      841 tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga
      901 taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt
      961 tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat
     1021 caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa
     1081 tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat
     1141 gcaacaaatc ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc
     1201 actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc
     1261 atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac
     1321 ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac
     1381 cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta
     1441 tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca
     1501 gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt
     1561 gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc
     1621 atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct
     1681 tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac
     1741 attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt
     1801 ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga
     1861 ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca
     1921 cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt
     1981 tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc
     2041 ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat
     2101 gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa
     2161 tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct
     2221 acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta
     2281 agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat
     2341 attggagttt tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa
     2401 ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa
     2461 agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc
     2521 tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta
     2581 gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat
     2641 ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa
     2701 tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg
     2761 atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt
     2821 gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta
     2881 ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct
     2941 attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga
     3001 gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc
     3061 acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg
     3121 acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac
     3181 tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact
     3241 gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc
     3301 tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt
     3361 catattgtat tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa
     3421 aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca
     3481 ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat
     3541 gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt
     3601 aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg acatctttat
     3661 cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca
     3721 aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa
     3781 gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg
     3841 tataagagga tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag
     3901 aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga
     3961 ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag
     4021 ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag
     4081 agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg
     4141 cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga
     4201 tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg
     4261 aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta
     4321 atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt
     4381 tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg
     4441 ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata
     4501 cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt
     4561 ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac
     4621 tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg
     4681 tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac
     4741 ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca
     4801 tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt
     4861 ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat
     4921 aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg
     4981 tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt
     5041 gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc
     5101 catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca
     5161 taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa
     5221 atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca
     5281 tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat
     5341 cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct
     5401 tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt
     5461 ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg
     5521 atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata
     5581 tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct
     5641 tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta
     5701 tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg
     5761 acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga
     5821 agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct
     5881 ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat
     5941 tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta
     6001 cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg
     6061 atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct
     6121 gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct
     6181 acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa
     6241 agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg
     6301 tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga
     6361 taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta
     6421 gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc
     6481 acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt
     6541 attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa
     6601 ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg
     6661 aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat
     6721 tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag
     6781 agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag
     6841 ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa
     6901 gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga
     6961 atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc
     7021 gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg
     7081 gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa
     7141 tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca
     7201 aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg
     7261 tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact
     7321 attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat
     7381 aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa
     7441 aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa
     7501 tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca
     7561 ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat
     7621 aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc
     7681 gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact
     7741 gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat
     7801 gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag
     7861 ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa
     7921 tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg
     7981 aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt
     8041 tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac
     8101 tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct
     8161 tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca
     8221 aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg
     8281 atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc
     8341 ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag
     8401 cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt
     8461 cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta
     8521 aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct
     8581 agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa
     8641 aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga
     8701 ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga
     8761 actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt
     8821 atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg
     8881 atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg
     8941 ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg
     9001 ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa
     9061 ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta
     9121 aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct
     9181 tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta
     9241 aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt
     9301 tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac
     9361 tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta
     9421 tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag
     9481 atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta
     9541 agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag
     9601 caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag
     9661 aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt
     9721 caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa
     9781 ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca
     9841 ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg
     9901 tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga
     9961 ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg
    10021 gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta
    10081 aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca
    10141 tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat
    10201 gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa
    10261 cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga
    10321 aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag
    10381 atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc
    10441 gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct
    10501 caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa
    10561 tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag
    10621 ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg
    10681 aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa
    10741 cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc
    10801 ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt
    10861 tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa
    10921 aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc
    10981 atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga
    11041 caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa
    11101 agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta
    11161 ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca
    11221 gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa
    11281 agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt
    11341 tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca
    11401 cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat
    11461 attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa
    11521 ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat
    11581 gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat
    11641 cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt
    11701 gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt
    11761 gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct
    11821 ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc
    11881 catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca
    11941 ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac
    12001 acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca
    12061 gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact
    12121 gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg
    12181 aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa
    12241 actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct
    12301 atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata
    12361 agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat
    12421 cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga
    12481 aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga
    12541 caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc
    12601 tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat
    12661 tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat
    12721 tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt
    12781 gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat
    12841 ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat
    12901 ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt
    12961 actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt
    13021 gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga
    13081 tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac
    13141 agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt
    13201 ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa
    13261 tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg
    13321 tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca
    13381 agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat
    13441 cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg
    13501 tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta
    13561 tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc
    13621 cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc
    13681 tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga
    13741 tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca
    13801 taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt
    13861 agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg
    13921 tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac
    13981 cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg
    14041 aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg
    14101 cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg
    14161 cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt
    14221 ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg
    14281 gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc
    14341 caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag
    14401 cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca
    14461 tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa
    14521 gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac
    14581 catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca
    14641 tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt
    14701 ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg
    14761 ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag
    14821 gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg
    14881 ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca
    14941 gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg
    15001 tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg
    15061 tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga
    15121 aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc
    15181 cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg
    15241 aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg
    15301 tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc
    15361 atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt
    15421 agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc
    15481 acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat
    15541 cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc
    15601 aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc
    15661 ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc
    15721 tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat
    15781 ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt
    15841 ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa
    15901 gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag
    15961 tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc
    16021 tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa
    16081 agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt
    16141 tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa
    16201 gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga
    16261 gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg
    16321 aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct
    16381 cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt
    16441 tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact
    16501 ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt
    16561 tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac
    16621 ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt
    16681 cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc
    16741 gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac
    16801 gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca
    16861 ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga
    16921 aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat
    16981 atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt
    17041 accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg
    17101 catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac
    17161 gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat
    17221 acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg
    17281 cctcaaggat attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg
    17341 actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc
    17401 aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag
    17461 taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa
    17521 tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag
    17581 ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc
    17641 aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg
    17701 tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg
    17761 aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa
    17821 atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag
    17881 cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc
    17941 ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc
    18001 gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg
    18061 tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag
    18121 gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat
    18181 cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg
    18241 agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat
    18301 cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag
    18361 gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga
    18421 acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt
    18481 gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt
    18541 gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa
    18601 gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct
    18661 aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa
    18721 gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa
    18781 gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc
    18841 gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc
    18901 aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa
    18961 gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact
    19021 tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
    19081 tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    19141 gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg
    19201 actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt
    19261 agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag
    19321 atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt
    19381 accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg
    19441 ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg
    19501 tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat
    19561 taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt
    19621 ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg
    19681 ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt
    19741 tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt
    19801 gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc
    19861 ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa
    19921 ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg
    19981 ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag attctatgag
    20041 aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta
    20101 ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc
    20161 aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag
    20221 acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt
    20281 tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt
    20341 tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc
    20401 atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg
    20461 ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat
    20521 gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt
    20581 ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc
    20641 aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca
    20701 gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt
    20761 tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt ttctttattt
    20821 acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac
    20881 catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa
    20941 gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt
    21001 ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa
    21061 aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc
    21121 aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc
    21181 gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact
    21241 gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa
    21301 cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat
    21361 acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga
    21421 accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc
    21481 taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct
    21541 cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt
    21601 tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag
    21661 gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga
    21721 taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg
    21781 aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta
    21841 cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat
    21901 aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact
    21961 cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt
    22021 cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca taacttacaa
    22081 gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac
    22141 catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct
    22201 aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt
    22261 ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac
    22321 gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag
    22381 taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta
    22441 tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg
    22501 aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt
    22561 caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt
    22621 gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag
    22681 aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata
    22741 aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg
    22801 tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga
    22861 gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta
    22921 tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg
    22981 gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac
    23041 aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc
    23101 accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt
    23161 cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc
    23221 aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa
    23281 acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt
    23341 tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa
    23401 ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg
    23461 aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg
    23521 ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa
    23581 tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc
    23641 cagtactatg tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc
    23701 ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg
    23761 atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag
    23821 ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac
    23881 aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc
    23941 tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg
    24001 ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag
    24061 ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat
    24121 caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta
    24181 ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac
    24241 ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag
    24301 gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt
    24361 ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa
    24421 atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg
    24481 aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg
    24541 gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt
    24601 caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag
    24661 ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt
    24721 taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc
    24781 gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg
    24841 ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact
    24901 acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg
    24961 aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta
    25021 atatagtttc gtgtaaaata attatcgatg atatggg
//
LOCUS       DAHEYX010000014        24676 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHEYX010000014 DAHEYX010000000
VERSION     DAHEYX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.014"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(97..225)
                     /locus_tag="MB219_000403"
     CDS             complement(97..225)
                     /locus_tag="MB219_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCC8426140.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(228..1601)
                     /gene="rlmD"
                     /locus_tag="MB219_000404"
     CDS             complement(228..1601)
                     /gene="rlmD"
                     /locus_tag="MB219_000404"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCC8426141.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1712..2767)
                     /locus_tag="MB219_000405"
     CDS             complement(1712..2767)
                     /locus_tag="MB219_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCC8426142.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2777..4207)
                     /gene="gatB"
                     /locus_tag="MB219_000406"
     CDS             complement(2777..4207)
                     /gene="gatB"
                     /locus_tag="MB219_000406"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCC8426143.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4204..5676)
                     /gene="gatA"
                     /locus_tag="MB219_000407"
     CDS             complement(4204..5676)
                     /gene="gatA"
                     /locus_tag="MB219_000407"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCC8426144.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5676..5981)
                     /gene="gatC"
                     /locus_tag="MB219_000408"
     CDS             complement(5676..5981)
                     /gene="gatC"
                     /locus_tag="MB219_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCC8426145.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6087..8123)
                     /gene="ligA"
                     /locus_tag="MB219_000409"
     CDS             complement(6087..8123)
                     /gene="ligA"
                     /locus_tag="MB219_000409"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCC8426146.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8140..10374)
                     /gene="pcrA"
                     /locus_tag="MB219_000410"
     CDS             complement(8140..10374)
                     /gene="pcrA"
                     /locus_tag="MB219_000410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCC8426147.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10529..12088
                     /locus_tag="MB219_000411"
     CDS             10529..12088
                     /locus_tag="MB219_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCC8426148.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12199..14130)
                     /locus_tag="MB219_000412"
     CDS             complement(12199..14130)
                     /locus_tag="MB219_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8426149.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14148..15062)
                     /gene="pfkB"
                     /locus_tag="MB219_000413"
     CDS             complement(14148..15062)
                     /gene="pfkB"
                     /locus_tag="MB219_000413"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCC8426150.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15059..15811)
                     /locus_tag="MB219_000414"
     CDS             complement(15059..15811)
                     /locus_tag="MB219_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCC8426151.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15970..16179)
                     /locus_tag="MB219_000415"
     CDS             complement(15970..16179)
                     /locus_tag="MB219_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426152.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16190..17518)
                     /locus_tag="MB219_000416"
     CDS             complement(16190..17518)
                     /locus_tag="MB219_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426153.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17654..17809
                     /locus_tag="MB219_000417"
     CDS             17654..17809
                     /locus_tag="MB219_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426154.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17883..18470)
                     /locus_tag="MB219_000418"
     CDS             complement(17883..18470)
                     /locus_tag="MB219_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCC8426155.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18553..19803)
                     /gene="clpX"
                     /locus_tag="MB219_000419"
     CDS             complement(18553..19803)
                     /gene="clpX"
                     /locus_tag="MB219_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCC8426156.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20050..21339)
                     /locus_tag="MB219_000420"
     CDS             complement(20050..21339)
                     /locus_tag="MB219_000420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCC8426157.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21493..22425)
                     /locus_tag="MB219_000421"
     CDS             complement(21493..22425)
                     /locus_tag="MB219_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426158.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22582..23241)
                     /locus_tag="MB219_000422"
     CDS             complement(22582..23241)
                     /locus_tag="MB219_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCC8426159.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23244..24269)
                     /gene="sppA"
                     /locus_tag="MB219_000423"
     CDS             complement(23244..24269)
                     /gene="sppA"
                     /locus_tag="MB219_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCC8426160.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24282..24572)
                     /locus_tag="MB219_000424"
     CDS             complement(24282..24572)
                     /locus_tag="MB219_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426161.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
BASE COUNT         6565 a         5342 c         4338 g         8431 t
ORIGIN      
        1 tttgtacaac ccatcagtcc ttattgacaa agagccacta aaaagactaa gaaacaataa
       61 caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga ttttagggcc
      121 ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc ggcctccacc
      181 ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta ttctccttta
      241 acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac aggctgaact
      301 ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa agttgccgga
      361 ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc aataaaatga
      421 tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat ccctttttct
      481 tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt ttcgatttta
      541 ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc catcccatac
      601 accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga ataagcgtcg
      661 atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta cagatactct
      721 gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt ttttcctaat
      781 agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc taaaatcaca
      841 tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa ttcttcatga
      901 atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac taaaacgacc
      961 atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg tccttcattt
     1021 ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac tactttgatt
     1081 gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag atcatggcta
     1141 ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg gatctgtgct
     1201 ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg aacttctggc
     1261 atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac aagctgttgt
     1321 tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt gttttctaca
     1381 ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc aaacttattg
     1441 cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc gataaataat
     1501 ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac gatttcgact
     1561 gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc ctttaagcct
     1621 atttttcaat cctactgaac tctactataa atcagctaat tacacaacta aaaccggcac
     1681 tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt gtttcgtctg
     1741 ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct tttacgaatt
     1801 ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca tcaggaatct
     1861 gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc atcggtgctt
     1921 ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca tcttctgttt
     1981 caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca tgtcgaccgc
     2041 cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc ttcacaatga
     2101 ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg aatcctccaa
     2161 cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc ccttcatcat
     2221 acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct ttcgcaagat
     2281 aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc aattctgtca
     2341 aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg atgtccattt
     2401 ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct ctaggaattt
     2461 ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt ttaggacggt
     2521 gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca ccagctgcca
     2581 ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct gaatctggtt
     2641 ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc tgtaggatat
     2701 ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt acgcgtgctt
     2761 ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta ttcacgacac
     2821 caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag ccaaccgcac
     2881 gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa atttcattaa
     2941 tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt tcaactactt
     3001 cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg atttttgagc
     3061 tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt gtcaattttg
     3121 tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc atcaaccagt
     3181 tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa tcagacattt
     3241 cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca cggatatagc
     3301 gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga atccattcgt
     3361 catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca ctagaccctt
     3421 ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt gtttcttgtt
     3481 ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa gctaaacctt
     3541 ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg aactcttctt
     3601 gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct tccattttca
     3661 catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa gcgtatgctt
     3721 cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg ccttgtcggt
     3781 tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca tcttcttcta
     3841 aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt tcgatccatc
     3901 cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga ttatctgggt
     3961 aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt aatgccaaag
     4021 ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg cctggataac
     4081 cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct ggcgctggag
     4141 agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg actgtttcaa
     4201 aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt ctgttgcttg
     4261 ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac cgatgatctg
     4321 caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg gaagtccagc
     4381 caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat cgttaatgtt
     4441 ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt cataatctgc
     4501 aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcccgctt ttttgaagta
     4561 agcgtcataa taaccagcac ttaaggagaa tgtacctaac atgatacggc gttttacttc
     4621 ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt tcacattttc
     4681 tgagcgataa ccgtagcgga taccatcgaa tcgttgcaag ttggaacttg cttctgatga
     4741 ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga cttcttccac
     4801 aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt ttacgccagg
     4861 ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt tgatgtcgcc
     4921 tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt ctttttcgtc
     4981 ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg tcattggtcc
     5041 gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac ggccataagt
     5101 aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc ccccagtatc
     5161 actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac caccagaaga
     5221 gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat aagaagtttc
     5281 tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga tcatatctgc
     5341 ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt aaaggatctt
     5401 agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac caattggaat
     5461 accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg cctgttccaa
     5521 tgctttttct tcgttcaatg taatgaatgc ttcgacctca ggctctgttt ctttgattcg
     5581 ttcaaatgtt gcttttgtca aatcttgtac agtgatttct ttcgatacaa gcatatgatg
     5641 cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc cccattatcg
     5701 atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt caataattca
     5761 tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt ttcaaccaca
     5821 ttcgacgtaa atggaacacc ttctgtatct acttcttcca gcatttcaac catatcgatg
     5881 attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc aagttttgct
     5941 aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct tctttctaaa
     6001 cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa attgtttatt
     6061 gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt agccaatgca
     6121 tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt ggtcagcttg
     6181 ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct accagtcact
     6241 ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg tgttagttta
     6301 ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa ttgggcattt
     6361 cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat caattcatgg
     6421 acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc gatcgtatca
     6481 agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc gaaatgttct
     6541 gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa aatcaaccgc
     6601 tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc tgatttttct
     6661 tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc agcaacatct
     6721 gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc atcgatattc
     6781 atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg gcatttagga
     6841 ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc acaaactgga
     6901 caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa cacgacttgc
     6961 gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt caagcggata
     7021 tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt cgttcctgct
     7081 actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc gactgtccat
     7141 tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc tgctgcccat
     7201 ctcggtgctt tgacagtaaa accaagttga tcttgtagcg agaattcatt cactttgatg
     7261 acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc ttcaatgtat
     7321 gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg gaatccaatt
     7381 ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc gaaatcagct
     7441 actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc taactgacgc
     7501 aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc tgcttctctg
     7561 tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct tacttcgatg
     7621 ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt ttcagtgatg
     7681 ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc atcttcataa
     7741 cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac tacacgtccc
     7801 actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa aacgtcgttc
     7861 aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac ttttccgcct
     7921 actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc aattgctact
     7981 aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc ttctacgtag
     8041 tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt agcagcttcg
     8101 tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt caattggcgc
     8161 aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta gttctagatc
     8221 cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt gtttgacttt
     8281 atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg gaacaaccgc
     8341 tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggtg tttttttagg
     8401 cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta caaaacgaga
     8461 aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg ttaaatacaa
     8521 cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttctt ccagttcgct
     8581 ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa tcaggaatac
     8641 aactgggaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt gggaagcatc
     8701 ttcttctaaa ttgtcaatat cggaaacaag tgccagatca tttaagaaga ccgttagttt
     8761 ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt cgaattcttg
     8821 tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta atgtattttg
     8881 gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca attctgtcac
     8941 tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg cacctaattg
     9001 ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt caagcagtgg
     9061 ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc cgatgcctcg
     9121 tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggatttg ccagcacatt
     9181 caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc ctccaaccat
     9241 tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt gtgcgttcgt
     9301 ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct cttcttgcat
     9361 ccgatctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt aagtgatctt
     9421 gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat tgttttcaat
     9481 gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt ctagcaaaat
     9541 tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat ctgcaccgcg
     9601 ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa atctacctgc
     9661 caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat ctacatgaat
     9721 ataatggaat ttgttttgat aatatgtgag tgaatcagga tgttcttcaa ataatcgaat
     9781 cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct ctttttggta
     9841 agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt ttgcaaaatc
     9901 ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac ttcgaggatc
     9961 atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg tcagctgttc
    10021 agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat ctcttcttaa
    10081 aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc cagatgcgag
    10141 aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag taatcgctag
    10201 gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt gtgtcagcac
    10261 tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag tgtgaagaac
    10321 agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg acattaacaa
    10381 catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca ttaaactagc
    10441 tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt tgatttcgcg
    10501 ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca agaaattaga
    10561 taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga caggaatcgt
    10621 cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa gtatctatga
    10681 aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca tccttgcagc
    10741 atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg ggattccaca
    10801 agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt tatggaaaaa
    10861 gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag aaggaccttc
    10921 tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga gtccatcttc
    10981 agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag cattcaatgc
    11041 tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt ctccacttgt
    11101 ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt actttttcgg
    11161 cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat attatggttc
    11221 cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga aggtcttgct
    11281 ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg ggttcgttcc
    11341 tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg gaggcaatca
    11401 aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg gtttgtttgt
    11461 tcttcgattc gttttttcca tgatttctta cggtggtggt cttccagggg ggattttcct
    11521 tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta tggttgattt
    11581 cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg gttatttcac
    11641 agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg ttggctctct
    11701 taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca atgaccttct
    11761 cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata ctcagaccgc
    11821 ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga gttctttaga
    11881 cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct ctatacgcag
    11941 aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag atattttggt
    12001 tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg ctaaatcttc
    12061 tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta caacaactat
    12121 tcatctacac tacttgattc tatatgattc tataaaaaaa gaagagactg ggaaaaacca
    12181 gcctcttctt ttttatttct atgcttctgc tgctgcagga gcttttctca ataaaccata
    12241 aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga ccgggtgact
    12301 cagtaagaat acaacgaaaa tcccgccatg aggagccagt aatttgatcc cgaaagctcc
    12361 gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac gaatcgggtc
    12421 agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca cgatgttcgt
    12481 caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg tagcaacgaa
    12541 aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga cacttccgcc
    12601 agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt tgttgattgg
    12661 accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag cagcattcga
    12721 accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca tagggacatt
    12781 gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa agaggatcgt
    12841 tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa atacgatgac
    12901 ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc ctgtattagc
    12961 taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac ttgaagcaat
    13021 aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt ggttgaacca
    13081 gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca cgccaataaa
    13141 ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga aaggtaacat
    13201 gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac catccgcatt
    13261 tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag taccactaag
    13321 ggctagattg atcagctctt ctggtttacg gataccatca ctgacaggac gattgatcag
    13381 ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga ttacaccttc
    13441 tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac cgttcgtttc
    13501 gactttgatc tccacaccca tttctttcgc ttttttcttc agagcatctt ctgccatgta
    13561 agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc tttctggatg
    13621 agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt ctttttgctc
    13681 ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt ttaattttcc
    13741 tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca aatgtgtgtc
    13801 gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc cgtctaaggc
    13861 ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt taactgcact
    13921 gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt gtgcttcgcg
    13981 ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat caaacatttt
    14041 ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca tgatcatcgc
    14101 ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta atcaattttt
    14161 gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa gtcatcagaa
    14221 aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg atctttcgta
    14281 cggctgtaag ccccaataaa gccggcaatc attgaatccc ctgcacctac tgaatttttc
    14341 aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag caaagcaccg
    14401 tctccagcca ttgagatcag cacattctgt gccccttcag ctagcaattt tttaccatac
    14461 ggaatgattt cttctacaga ttggaaggtt gtattgtaaa gctcagctaa ttcgtgattg
    14521 tttggtttga tcaacaatgg tttcttaaca agtgcatttt ttaggtcatc acctgttgta
    14581 tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg ataaaatcct
    14641 tttcgcaggc tagccggcgc gcttcctgac aatacgacga tatctccttg tcctactgtt
    14701 cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc caaaccgtta
    14761 atttcagttt cagtatctga ttttagtttg atattgattc ttgtatcttc ttttacggtt
    14821 gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc gccagtgaac
    14881 cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat acgggaaaca
    14941 ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa atcgcctagt
    15001 tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac tgtgtaaatc
    15061 attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat tctaatggac
    15121 aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag gacaactggt
    15181 tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg gctaaacgtt
    15241 taacagctgc ctcttctggg tccggagtcg tgaatccttg ttcaagatgg gcaccgttca
    15301 ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca ttagatccta
    15361 aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca agctccaaat
    15421 caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt tttccttgca
    15481 gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca tcttcgtcat
    15541 taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt ttgagtgatt
    15601 tttcactcat attctgctca aaattcaata attgctcttt cttagctccg ccatgaactc
    15661 tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt gattcagaag
    15721 cattaaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca agcaagtcta
    15781 ggattttttg tctacgttcc tctgtaagca ttttcacgat cacctcaata caaataatag
    15841 catatttttt ttctactcgc aatataaatc agtcaaaaac gttcataaaa attataaatc
    15901 agtcaaaaaa aaataaaaaa ccagtcaaaa agactggttt tttatttttt tgtctatcat
    15961 caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa cagaagcagg
    16021 ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca tgaccaaaaa
    16081 accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg ataataatga
    16141 tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct catatttttt
    16201 gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc agatgctgct
    16261 cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt ttgaaggtaa
    16321 catcagagag tctatgtttg atttccttta acggtaattc tgacagggat tctgaaggaa
    16381 catagattct taaggataaa agatactctt gatatttttc atctgttacc tctacttcta
    16441 gatattccct aatataataa caataagctt cgattccatt atccaaagca acagtcggat
    16501 cgataacttt atagcacatc atgcgcgagc gattctcgtc aacccaagga aaatacgcct
    16561 gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttga atatcataac
    16621 tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt gcattctgat
    16681 actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc ggtttcaata
    16741 aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta aaataggatt
    16801 gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc tggatacata
    16861 tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga aaaatcggtt
    16921 tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa gtgaaacata
    16981 aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact acttccccgt
    17041 tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac aatagccgtt
    17101 gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata taggcgaaca
    17161 ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc ttttctttga
    17221 aaacagagat agacaataca ttttttatca ataattgatt atcttccaag cgaaaactac
    17281 attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat gctagcatta
    17341 ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa gagcggataa
    17401 atgttggctg gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc tgtttttttt
    17461 cttcttcatt taagtctgtg agctggatag tcatcaataa tttatcttcg ccaatcattt
    17521 tctcacctct ttaaaatctt tttataattt aattataacc aacgataaag aaaaactaaa
    17581 actttatgtc ttagaaacca atttggacga ttacatgata gactaagtaa aaagaaacaa
    17641 agaggtgttt acaatggggt tgaaatttga acgtacagat aatttagata aacttttcga
    17701 atcatttgct gtcgatccgg aaaaaacagt tgcaccaaaa gaagagatcg atcgatttct
    17761 aaaaccacag ccacaaaatg aaaagaaaaa agacaagaaa caaaagtaag ttaagctcaa
    17821 aaaaagactt accagccaaa cttttatgaa agagtttgac tggtaagtct tttttattct
    17881 ttttatgatt caattgcttg ttcgattgct gcccacgctt cttcttttcc ctgttttgta
    17941 acggaagaaa agagaataaa cgtgtcattg cgatcaaaat ccaacgcttt tttgatttcg
    18001 ctctcatgct tgttccattt accacgaggg atcttatctg ccttagtggc aacaacaatt
    18061 acagggatat cataatactt caaaaattga tacatctgga tatcttcttg agaaggctta
    18121 tgacgcaaat ctactaaaga aacgaccgca cgtagctggt ctctagtcgt taaatatgtc
    18181 tcgatcatct ttccccattt tgctcgctca gttttggata cttttgcata cccatatccc
    18241 ggaacatcga caaaatgtaa tgcgttctcg atcaaataaa aattcaatgt ctgggtcttt
    18301 cctggcttac cggaagtacg ggctaaattc tttcgatcaa tcaatgtgtt gataaaagaa
    18361 gatttcccta catttgatcg tcctgccaga gctatttctg gcaattgtgt atctggatat
    18421 tgttttggtg ccactgcact tatgataatt tcagcttgat ggactttcat ggtctctctc
    18481 cttaacaagg tgatgaataa agcaagcggc tgtcgcaaaa gccaaaatga cttttacggc
    18541 agcctctttt catcaacccg cttttttctc tgaatcttta tagattactg ttggctcacc
    18601 agacaattca gctgcttctt ttgtgataat cactttttcg atgttttcat ttgaagggat
    18661 atcaaacatc acatccatca tgatttcttc aatgatggaa cgaagtcctc gagcaccggt
    18721 attgcgttcg attgcttttt tcgcaatggc tctcaacgct tcaggttcga attcaagttc
    18781 ggtattatct agagaaagaa gtttttgata ttgtttgact agcgcatttt tcggctcagt
    18841 caagatccgt accagatcat ctgttgtcaa tttctctaaa gctgccataa caggtaatcg
    18901 tccgataaac tcagggatca atccgaattt tagtaaatct tcaggaatga tatgctgcat
    18961 gacactttcg tcttcagaaa gtttttggtt ttgtgtacca aatccaatcg tcttttcgcc
    19021 catacgattc ttcacaatcg tttcgatgcc atcaaatgca ccacctacga tgaacaatat
    19081 gttcgtcgta tcaatctgaa taaattcttg atgtggatgt ttacgtcctc cctgaggtgg
    19141 cacgcttgca acagttcctt ctagaatttt cagcaaggct tgttggacac cttcgccgga
    19201 aacgtcacga gtaatagata cattttcgct tttacgcgca attttatcga tctcatcaat
    19261 atagatgatc cctttttctg cacgttccac attgtaatca gcagattgaa gcaatttaag
    19321 caaaatgttt tctacatctt cccccacata accagcttct gtcaaacttg tcgcatctgc
    19381 gattgcaaaa ggtacattca atgtttttgc taatgtttgt gctaaaaatg ttttaccaga
    19441 ccctgtaggt ccgatcaagc agatattgct tttttgcagt tcaacatcat ctgtgttttc
    19501 ttgtgcttgc tgattgacac gtttatagtg attgtatacg gcaacagcta acgagcgttt
    19561 tgcgcgatct tgtccaatca cgtagttatt caatacttca agaatttcta gtggtttagg
    19621 gacttcagtg aattcgcgta cagcttcttc ataaaattct tcatcaatga tttctttaca
    19681 taggtcgata cattcattac agatgtagac ccctggacca gctacgatct ttttcacttc
    19741 ttcttgggtt ttcccgcaaa aagaacagcg tacggtctca ttgctacttg gattgtcata
    19801 catggtcttc accccttaac ttgcaaaaag tatgcaagaa actatcaaag actttacagt
    19861 cttttactat cataacatag tttagtaaaa agaaaaagtg tttaacaaag gatcaactga
    19921 cctatacctt tgcatttttt actcttaaca aaaaataagg aactgtgaca aaatcgtgtc
    19981 acagtccctt atttcgaata aacaattttc tagaagtagc ttcttcagaa atgatttatt
    20041 acttgatttc tattctttcg cttctgtcgc tgtacctgtg atcaattcga ttgctttttt
    20101 cattgtcaca tcatgttcca gcatatcttc tgtcaacacg cgtttgattt gttcgattgg
    20161 catgttgtat gtttctgata attcttggat ttctgcattg atttcttctt ctgttgcttg
    20221 aagattttca gcagctgcaa ttgcttcgat cacaagattt gttcttgtgc gcatttctgc
    20281 ttcaccttcg aattgtttat gcaagtcttc ttctgtagaa ccagttaatt gatagtacat
    20341 ttcaggagag ataccttgac gttgcatgtt gttcaagaat tcgtccattg aacgatgtac
    20401 ttcatcatga accatcacgt gtggcaattc aacgatttct gcgttttcaa cagccatacg
    20461 gatcgctgct tcatcttttg catcgtccgc tgctttttct tttgtttcta ctaattcttt
    20521 gcgatatttt tctttcaatt cgtctaatgt tgatacttcg tcatcaacat cttttgcgaa
    20581 ttcgtcatct aattcaggca cttcttttgc ttttacttca tggattgtca ctttgaagac
    20641 tgcttctttt ccagcaagat cttctgcttg ataatcttct gggaaagtta catttacatc
    20701 tagactttca cctgatttat gaccaactaa ttgatcttca aaacctggaa tgaatgagcc
    20761 tgaacctaat tcaagtgagt aattttcgcc ttttccgcca tcaaacgctt cgccatctac
    20821 aaagccttcg aagtcgatta ctactgtatc gccattttca gctgcagcat cttctttgat
    20881 aactaattca gcttgtgctt cttgttcacg tttgatacgt gcatccacgt cagcatctgt
    20941 tacttcacgg tcttgttttt caacaactag atctttatat tgtcctaatt ttacttctgg
    21001 tttcactgtt acttcagcag tgattaccca gtcttgacct ttttccatgc tttcaacatc
    21061 gatttttggt tgagaaacag gatcgatgcc agcttctttg acagcagctt cataagcttc
    21121 tggtaataca gcattcaatg catcttcata taaagcttct tcaccgtaca tacggttgaa
    21181 tacttgacga gaaactttcc ctttacggaa tccaggtacg ttcaagcttt ttttcacttt
    21241 attaaacgca gttgttaatc ctttttggat ttcgtcttgc gcaatcgtaa attttaaaac
    21301 accatcattc gtgccttttt tttcaaatgt tgcagtcatt taattccctc cgagtttaga
    21361 aattcatttt atacatcaaa aacgtttcat gcataccttt aaatagtaca ctaacccttt
    21421 caaattgtaa acatattgaa tggatagaat ccttgttttt cataacattt ttatcttttt
    21481 cactgataaa atctaatgga agaagatcat ttgctgcaaa attttttctc ttttttttaa
    21541 aaaatcttgt ttttcatctt ctgtaccttc atacatcgtt tttcctgtac acgcctctac
    21601 agtcagttga aaccagcgct tcgggtcttc gatgtatgta tcatagacag gataaacaat
    21661 tgcagattgc aacgtgaatt cttcatttaa cacggaacta agcagcgcat cttcattacc
    21721 gtaacgatct gaaaatacac ataattgctg gtaacttgcc tgctgctgcg gctttggcag
    21781 atcttttggt atgactttga ctaacttttc ttcaatcgtc aaatagaaaa caggatggca
    21841 ttcgttcaat tgcctaagtg tctccagtaa ttttgccctt gctaaaatat ggacctctgg
    21901 cgatacaata agtttactcg ctgtttggat aaactcatga taaggaagct gttcgatctc
    21961 atcaatagac gcaagctgac gtgcaggttc caacgaagga agttcctccg atttattgtg
    22021 gagtgccttg atcttctgcc tctccatttg tccggataat tcttctgcaa attggatttt
    22081 ttcaatgacc cgctgctcga aagcttcata aaaatctgta cttgctatca attttctagc
    22141 atataaaaaa tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa actcctcatt
    22201 ttgaagatac tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt tctcgtccaa
    22261 ctcgtaaaga caaaaaacaa gcaggctatt agcctcaaag ctttctttca actcgtaagc
    22321 tcgttgaaat aattcttttg cctgctgata ttgctggttt gctagtgctt gttgtgctac
    22381 tcgcattaaa ttctcatatt tttctggaaa atcaatcggc ttcacgcagt gtcctcctct
    22441 tgctcatgtt cagctcatgt tcaatagtag caaaacaaaa aaagcatgac aattaaaaat
    22501 aactgaagca ccctctaaaa gctagatttt agatctgact tttagaaggt gctttcaact
    22561 gttactgctt tttattgctc atcagctgtt gacttcctgg ttgaatgctt tgatcagatt
    22621 gatcgtaaca acacttgtat tagtgaaaaa atcgcctaga tgttgctttt tctgattaaa
    22681 gattgctgga agatacccta acatcagcaa aggaaatttt agaatgaacc ggcaagcacc
    22741 ttctctaact aaaacggttg accagctaag ttcttcttca tcaaagctga tgactcgaat
    22801 accaaagatc attttcccaa tcgtttgacc atgattgaat ttcgtcaata aaataaaata
    22861 cgctagataa ataagtaagg ctgtgaatcc gtagatactc agataagaat ctgaaaaatt
    22921 aagccaacct agatttttgg caataccaag tgttaaacgc gtaatagctc cgatacacaa
    22981 aagatcgacc aagaaagcaa acattcggat aaaaaaacct gcatagaaat acttaggaaa
    23041 atcatttgct tttggcggtt catttcttgt gtccgtatac tttttccatt tacgttgttt
    23101 ctttttgatt tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta ccttttggga
    23161 aaagttggag tcaggtccta cagcaggctg ctgttgctta tctttttctt gttccttatt
    23221 ttcatttgaa agctctgaca tgcttactca cctccgtaat aatacattgc ttttggtgat
    23281 tccgctgtcc ctagattctc aagaacggat aagatctgac tagtctcaga aggcttcaat
    23341 ccctggaatt ctgctagttt acttcctaac catgtactag caaacccaga tggcgtggaa
    23401 gaatattcta ctagttccgc atcttcaagc tgttgttcct ttcgcatagc ttttaaagca
    23461 tcttcaggaa atcccaattc atcgactaaa cctacttctt ttgcttggac accatcataa
    23521 atccttccat cagcaatttt tttgacttct tcttcagatt tattccgtcc ctcgctgacg
    23581 attgctacaa aacgactata tgcgctgtcg atatatgctt gaagcaccgc ttcttcttct
    23641 tttgtctctg gtcgcgttcc tgaacccata tctttcaacg ctccgctttt aactgttgta
    23701 tcttccacac ccagtttttc tagaagtcct gaataattca atcctgacat gatcacacca
    23761 attgagccgg tcactgtttc ttctgttgca aagattttat ctgcttgagc agaaatatag
    23821 tagccaccgc tagctgccat gtttttcatg ctgacataca ttgggatatc atgttcttta
    23881 cgaatcgtat ccaatttctt agcgatttcc gcactttcgt agacaccgcc accaggagaa
    23941 tttacttcta aaaagatacc tttgactgtt ggatcttctt caatcgcctt cagttgttcc
    24001 ataaaatttt tatgattgta accttcagaa gcaaataaac ctgaagaccc tcccgcagca
    24061 atcgttccat ctactgataa ttttacgatt ttttgcgtac tgtctccctc ttccaacaca
    24121 tttgaagcta gttcttcatc cccatataac caactgttca atccctcaat ttgatcgttt
    24181 tcctgaggct tagtcaaact agctgaaact aacgaaacca caaataatcc tgcagcaatc
    24241 agaactgctg cccaacgtct cttattcatt ctttctcctt tctaatacaa gttttttatg
    24301 acgatcacag tttctgactg ctcagaagtg tcgaaccgct ctgctttttc ttcccgcgca
    24361 tacccggaaa acgactcttt tgtcaattca tcttctaccg taaactcacg agcttcagca
    24421 acttccaagg catcaggtgc gatttcttgg atgctatccc agccagtatc taaaaatatt
    24481 ttcgtatcat ctgatacacc agctgatgct tcgaatttgg acaattgttt ttttaacatt
    24541 cccagtgtca tgatctcaat ttttgaatcc atttttactc ctcctctatc cgattacttt
    24601 ctattgtacc gaactcctga aaaagatgat agataaaaac ccattatttt ttgacataaa
    24661 aagagcattt gacaaa
//
LOCUS       DAHEYX010000015        24148 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHEYX010000015 DAHEYX010000000
VERSION     DAHEYX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.015"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(144..293)
                     /locus_tag="MB219_000425"
     CDS             complement(144..293)
                     /locus_tag="MB219_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426162.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1261..1587
                     /locus_tag="MB219_000426"
     CDS             1261..1587
                     /locus_tag="MB219_000426"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8426163.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1568..2503
                     /locus_tag="MB219_000427"
     CDS             1568..2503
                     /locus_tag="MB219_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCC8426164.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3132..3383
                     /locus_tag="MB219_000428"
     CDS             3132..3383
                     /locus_tag="MB219_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HCC8426165.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3925..4083
                     /locus_tag="MB219_000429"
     CDS             3925..4083
                     /locus_tag="MB219_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426166.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4155..5381
                     /locus_tag="MB219_000430"
     CDS             4155..5381
                     /locus_tag="MB219_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCC8426167.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5470..5862)
                     /gene="rpsI"
                     /locus_tag="MB219_000431"
     CDS             complement(5470..5862)
                     /gene="rpsI"
                     /locus_tag="MB219_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCC8426168.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5878..6321)
                     /gene="rplM"
                     /locus_tag="MB219_000432"
     CDS             complement(5878..6321)
                     /gene="rplM"
                     /locus_tag="MB219_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCC8426169.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6840..7307)
                     /locus_tag="MB219_000433"
     CDS             complement(6840..7307)
                     /locus_tag="MB219_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCC8426170.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7427..8965)
                     /locus_tag="MB219_000434"
     CDS             complement(7427..8965)
                     /locus_tag="MB219_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCC8426171.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9045..9731
                     /locus_tag="MB219_000435"
     CDS             9045..9731
                     /locus_tag="MB219_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCC8426172.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9932..10459)
                     /gene="msrA"
                     /locus_tag="MB219_000436"
     CDS             complement(9932..10459)
                     /gene="msrA"
                     /locus_tag="MB219_000436"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCC8426173.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10543..11220)
                     /gene="pgmB"
                     /locus_tag="MB219_000437"
     CDS             complement(10543..11220)
                     /gene="pgmB"
                     /locus_tag="MB219_000437"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCC8426174.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11217..12347)
                     /locus_tag="MB219_000438"
     CDS             complement(11217..12347)
                     /locus_tag="MB219_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCC8426175.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(12347..14530)
                     /locus_tag="MB219_000439"
     CDS             complement(12347..14530)
                     /locus_tag="MB219_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCC8426176.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14527..15552)
                     /locus_tag="MB219_000440"
     CDS             complement(14527..15552)
                     /locus_tag="MB219_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8426177.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15597..16424)
                     /locus_tag="MB219_000441"
     CDS             complement(15597..16424)
                     /locus_tag="MB219_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCC8426178.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16421..17488)
                     /locus_tag="MB219_000442"
     CDS             complement(16421..17488)
                     /locus_tag="MB219_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCC8426179.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17514..18350)
                     /locus_tag="MB219_000443"
     CDS             complement(17514..18350)
                     /locus_tag="MB219_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8426180.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18347..19216)
                     /locus_tag="MB219_000444"
     CDS             complement(18347..19216)
                     /locus_tag="MB219_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8426181.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19286..20569)
                     /locus_tag="MB219_000445"
     CDS             complement(19286..20569)
                     /locus_tag="MB219_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCC8426182.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20583..22268)
                     /locus_tag="MB219_000446"
     CDS             complement(20583..22268)
                     /locus_tag="MB219_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCC8426183.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22543..23568
                     /locus_tag="MB219_000447"
     CDS             22543..23568
                     /locus_tag="MB219_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8426184.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7041 a         5011 c         4324 g         7772 t
ORIGIN      
        1 aaacattata atatgaatgt ttgtgaaaaa ataatacaac ttagtcattc tttacaacga
       61 tttatagaaa aacaacctct ttaaatataa aaggtacggg ttactgatta cttgtaaatc
      121 tttacgttcc agttttattc tttctaaaaa atatactcac ttcttttctt agacttcttt
      181 ttttgattct cctctttatg gtcttgtcct tttttgcctt caaaatattt aaataaaccc
      241 ctttcaaaag catttctagg ttttggacaa taaggaatga cttttttctt catataaata
      301 cctcatttaa ctatttgata tcatcacatt atcaaaaaaa ataggttttt tattaaaact
      361 atcttgacct ttcgggagat ttcactgtga tttattttta ttcccatact tttttcgttg
      421 gtcactttcg tatacctaat ctaagcgatc atcggcgacg gtgcttttac aacgaatggc
      481 aggacaaaac aaggggcgga aaaacttttt tgcctatttc ataaaaaact ttttcctaca
      541 gcaatccctt gttttgtcct accacacgtt gtgaacccaa agcacgccga cgaacgctta
      601 gctatacgaa aatgacctcc aaaaacaaaa aagtgaggtg aatagaaaca aaaagaaatt
      661 gaaatctccc aaaatgtcgt ccgcctcgga gacaacttgc tgctgaaaat ggttggttaa
      721 ttcatttaag tattaaataa gataaatcat caaattaatc ggaaaaatag catgattggc
      781 cttatcaaaa ataaactgta aaaacttata tttctatgag tggtggaaca atgctaatac
      841 caaattaatt tatcgattac ccacctgcac aaaccaaaga tagcattatt aggaaacgaa
      901 tcaatcagcg aatccagcat tataatgagg atactaaaaa taaagttttc ataattataa
      961 atccgccttc ctacgaaagg caccaatgag gtgatgaaaa ccttagagcg ctttctttct
     1021 ttttttattc cgtgttaccc tttccttgca ggtccctggt acactacaga acccgaggag
     1081 gtgagtggcg gggctgtata gcggcaaccc cgcataatta tatggtgtcg gtcatccgtt
     1141 aaggcatcaa tgaatggcgc aaacccgtag cccgtaaact tatctcttcc gaagttgact
     1201 cgatttcgcc ggatgaccag tccctaccaa acctgtacta tttttttaca ggaggttgct
     1261 atgttcaaaa tctttcgtaa aaattgttgt ggcttggatg tccacaaaac gtggatctat
     1321 gcgtgtatcg gaatcacaga tgcgaatggt cgtacagtgt ataagcaagc tcgcttctct
     1381 tcctttacaa aaggtctcaa tgaattagct gaatggcttg ctaaatacaa ctgtaaggat
     1441 gtgtgtttgg aatcttccgg taaattttgg attcctgttt ttaatattct tgaaaaaact
     1501 tgttttgtca cactggctca tccgaagtac accaaacccc agaaaggaaa taaaacagat
     1561 cgcaaagatg caaaaatgga tttgtgatct ctttatgtgc gacatgatca aacccagttt
     1621 tattcctggt cttgaaatcc gccagcttcg tgatctaatg cgttatcgga cgaaactgac
     1681 caacatgaaa acaagtgaaa agaatcgtgc tttaaactgc ttaaccgttt ctaatctgaa
     1741 actagatgat gtttttagtg atgtgttcgg aaaatcatcc acttctatca tccaatactt
     1801 gttagaacat cccggtgaat catttgatgt tcgaccattt atccatgccc gttgtaaaac
     1861 tcccgttgaa gaaatccaag cggcagttga gggggcgatt tctgtacctc aagcgattaa
     1921 gcttcgccaa tgcctcaacc atattaacga gattgagaag catcgagaag agatcaatct
     1981 agagattcaa gctcttgcag aacccttctc ccctgttttg gaactacttc gtaccatacc
     2041 gggcttagac actcagccaa taacagcgat tgcgattctt tcagaaatag gaccggatat
     2101 gtccgtgttt ccaacttcga aacactttat ttcttgggct ggatgctgtc caagaaacga
     2161 tcgcagtgcc cgacgaacaa aatcaacacg tatctctcgt gcagggcagt cgttgaaacc
     2221 gctacttgta cagattgcca atgctcttct cagatccaaa gaacatccag aatttaaaaa
     2281 tcgctatcgt agaatcaaag cgaatcgtgg acacaagaaa gctatcattg ctatttgtaa
     2341 gatgctcctg accgctatct ggaatgtctt atcaaaggtg gaaccctatt ctgctaaagg
     2401 atatctggag ccaaaacctg cgaaagaaga gaaagttctg acgcaatccc aagctctgga
     2461 ccttttaaga aaacgcggat ataccattat agatagtgct taaaccaaag ttacgttgat
     2521 tcttcctaaa tagactcgtt gtcttccatt tcaaaaattt tgaaaacggt aggcttattt
     2581 agtgtaccgt tcttcaggta tcttcaaata gatttatttt caaacttatt tcctttcttc
     2641 ttttactctc ctatagctta gcatttaagt gggcactcgt gtcaccttgc tagatagcta
     2701 tgaatgccgt aataagcctc ctagtatata ggcagactgc caattataac tgattcaatc
     2761 cacattatga taaaatccac cttttcgttt catatgtgtt gaaatatcac atatgtcaaa
     2821 agtgcacgag tcttttcatt cagtgctttg taaatactca aaattattcc ttttcacttt
     2881 aaaaaaaaga taattagtac aaaatcagaa atgaggaaag tattaggatg gactagaaag
     2941 gagttgttga atttgttgca acagattggt catttatcaa ttaaaaatta ggtgaagttt
     3001 tattcatcac tgcagctgct gtttcccaga gaataaaacg tctagaagaa actggtcagt
     3061 tactgtatta tcaagcagca attgactacg aacaagcttg attactaacc aaagcgttta
     3121 tccacctgtt gattaaatca aatcaaaaat caggttttta tacgttcata aaagcaatct
     3181 ccagtgtact cgaatgtgac tgtatcatag gtccttattc gatgttaatc aaaatggggt
     3241 ttccttctac cgaatcattg gacagtttta tcaatgattt atcaaagttt ggacgaacca
     3301 acacccaaat cgttttctcg actcttgttt ccttaatggt ttatcagttc tggaatgttt
     3361 atcatgcgct tttattaaaa tagaaaagag cttttttcat gcgacaaagc agtgatcatt
     3421 tattggcaca tgcaatcttt gtggatccat tttttgaaaa tacgatattt agttttgacc
     3481 caaaaatctt taaatacgta tttgataatt tatcttaaca accagataat tttattcagc
     3541 accgtattat ctatattgaa ttctcggaat caagataaat ttttttccgt ttttgtttct
     3601 tttttaatct tcttcttgtt attaatagtg attaggatga tattcctata ttgatctttg
     3661 ataattagat ataatttttg aataacgttc cctgctattc gagctagaaa tacaagtacg
     3721 ccatgaccta gttagcgaca actacttaga gatttctttt gttctgggac aacaaaatgc
     3781 catgacaata gcagtaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
     3841 gaaattccta tgtgcttacc caaagcggtt caaaaatttt aaactattat tttaggagat
     3901 gattttataa aatcgaaagc ctacttgaat aaaaaacaag cctgtgaatt tctaggaatt
     3961 gataataata ctttagataa gtggattcgt gtatataatt ttccagtaat aaaaattgat
     4021 ggcgtagttc gctttgacgt agaggatatc aaagatttca tgaatacatt caaaaaaaat
     4081 tgaaattttc ttctcgtcac atatactata aatagcgaaa tgtactgcct tactttttaa
     4141 aggagtgagg aagtttgcct acaaagcttt caaatgggaa atataaaact aatctgaggt
     4201 atccaaaaaa attcagagaa atcactggaa ttgcatcaga gaagtatcaa aaagtatttc
     4261 caactaggca attagctatt aaggccgaaa gggaaatgaa aaagaagatt gaaacagttt
     4321 taagagagga gaatgcaaac tcactagaat tgaaaggaaa aataaaattt aaagattttt
     4381 atgaatcaaa atggcttcct agatatgaac taggtcaaac tactaggtca aacagggctc
     4441 caagtaatgt aacaattagt aatactaagg atatattccg attgcacatc cttccaatgt
     4501 ttggtgaata tgcaatgaat tatctgaatt caaatactga gataatatca gatgaattaa
     4561 caaagaaatc gaaagaatac gcaaatataa aaattatcaa aggctatgtt cgctctatgt
     4621 ttgatattgc cgaaatacta tgttatatcg aattcaaccg aactaccaaa attattcaat
     4681 gtattaccgc gcctaagaaa atggctttag aagaaaaaag gataagggaa gggaatcaag
     4741 cattatcatc agaagagctg actgattgga tagaagcagt aaacaatgac ttaaataatc
     4801 acttgcttac acttcacgat tacacacttt tcatgcttac cttatatcta ggtgatagaa
     4861 aaagtgagac ttatgctctg caatggaaat atattgattt taataaaaat acggttcgtc
     4921 tcaaatatgc tttagataaa tatcaaagaa agacacatac aaaaggttgg aaagacaccg
     4981 ttattcaggc accagaagtt gtgatgacat tattaagaga atggaaatca gctcaggctg
     5041 aacaattgtt gaaactaaaa attatacaaa cacctgacca gtacctcttt acctattcca
     5101 aaccttctgg agaagtaaat tgtcctgttc acgctgacta cttaaactat cgaatcaatg
     5161 caataaaaaa aaggcatcct gaattggctc aattatctcc acacaaactt cgtcatacct
     5221 atgcaactat agctagggaa ggtggagcaa atatgaatca aatctctaat gctttaacgc
     5281 actcggatat ttctactaca aaaatatacg tgaacacacc tgacgtagtt gataaatctg
     5341 tttttgaagc atttcaaaaa ggattgaata agtgtgatta acatgtgttc tcaaatgtgt
     5401 tcttattata aaaaaagaca ctttccaaat aattgaaaag tgtcctgaaa tattgatata
     5461 aaaagaatat taacgttttg agaattgtga agctttacga gcttttttaa gacctggttt
     5521 tttacgttcg accatacgtg cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg
     5581 gaagtcagga tcaacttcta gcaatgcacg agcgatacca tgacggatag ctcctgattg
     5641 tccagcgtat cctccgccgt ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt
     5701 aacagcgaaa ggttgattga ttacttcacg caagtcagca tgtggaatgt attcttcaac
     5761 atctttttta tttacagtaa ttttgccagt tcctggtact agacgtacgc gagcaactgc
     5821 gtttttacga cggcctgtgc cgatatattg aacttgtgcc aatgaaattc cctcctatta
     5881 gattaagttt gtgatgtcta aaacttctgg ttgttgtgct gcatgtggat gttctgctcc
     5941 accgtatacg ttcaattttg taaattgttt gcggcctaaa gtgtttttag gaagcatacc
     6001 ttttacagaa gtttcgatca aacgacgaga atttttagca cgcaattcgc cagcagtgat
     6061 tgatttcaat cctcctgggt agttgctgtg acggtaataa attttgtcag tcgctttttt
     6121 acctgttaat ttcactttgt ctgcattgat gacgatgacg aaatcgccag tatccaaatg
     6181 aggtgtgaaa gttggtttat ttttaccacg tagtacagat gcaacaactg ttgaaagacg
     6241 tcccaatgga acatcagttg cgtctactac ataccattta cgttctactt cgcctggttt
     6301 ggccatatat gttgtacgca cgttattttc ctccaattta agttctgatg tttgacatac
     6361 acttcgagtt tccggggctc tttgtggggc aaacaatacc atttaacata ttacccgcta
     6421 atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat ttatatgaaa ctttacttct
     6481 tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt
     6541 gacaaaagtc ttgtcacaac cccttatttt gaataaacgg tgttctcctg ccaactcctc
     6601 ggtcacaagt cacaatatcc ataaaacata agaagctgtt ttttaaaatt gtttcttgtg
     6661 actcggagct gaacggcagg cttacaacct cttggtaaac ggtgttcaat cagctgaccc
     6721 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgacgttt gtttctctaa
     6781 tactcaggtc aaaacgctga tttcacaacc tctttttcgg tcaagttcaa caacagtttt
     6841 tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga tcatccagat acgtttttct
     6901 gtggctgttt tgaattcgat aaagatatct tgtgttggat cgtcgccttc ttctccagaa
     6961 acttcgattc ctttttggta aagatctgct agataacgat agcctgcaac aagtgtttcc
     7021 aaacgttctg gaatagtacg atcccagcgg ccaatttcat cagggatttt tgtgttatct
     7081 gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact
     7141 tcatctaact gagcgttcaa atcatccatg aactcatcca tcattgggtg caatgtaagg
     7201 aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa caacagacat ttggcttaga
     7261 tctgcaacta attgatttaa tacttctttg gtttgttgga atttcatctt tcttgcctcc
     7321 taacattttc ttacacttaa aataatagta gactttcaat gaaaagtaca acaaaatcat
     7381 ttacctgaga acgattttca gttcgctttt ttatccgtaa agccaatcat ttttcagaat
     7441 cagctaattc ttctcgcagt tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg
     7501 tcgaaacaaa taatggatag attttctgat attttttatg atcagtttct tgtactccaa
     7561 cttgctcttt caattctatc aattcctgtg cttcactgat actttggatc tttccgatgc
     7621 ttctccagcc taaaatcaca gctcctaatg cggatgcttc tgtgacagta gggaaaacaa
     7681 gcgggtaacc cagtacatca gccatcatct gacgaaaaat cggagattga gcaaacccgc
     7741 ctgtagcacg gatctctgtg agctgatttt cctggcaaac tccttttaaa atccgataaa
     7801 ggttgagaca gatcccttct agaatcgcct tcatcatttc tttttctgta tgttgccgct
     7861 gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta
     7921 aatagggatg aaaaatcaga ccattggcac ccgctggcac aaaagcgata gccgaaagca
     7981 gtgcatcata tggattttct cctttttctt tagcagattg aacgacttcc tgtaaaaatg
     8041 tatgccctgc ccaatcaaag atgccagcac cattgctagt cgctcctccc actacccaat
     8101 gtttctgatc cagaacataa caaaacgtct ccatttctgg atggaactga ggctgagtag
     8161 taataaaacg aagtgctccg ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag
     8221 cacctaaccc tagattagaa agcggcccgt ctgcccctcc caaaacaaat gatatatcat
     8281 ttggcaaacc tagttctgct gctgtttttg atggtagacc tgttacttga taggtggaag
     8341 gaaagacttg cggcaattgc tctattcgaa tgttcaagta gtcaagcact tcttctgccc
     8401 attggaattc atggatatca aaatatccag ttcctgaagc tgttccgtag tcgctgatca
     8461 gttcgccaaa caatctataa aagatatagt ctttgatacc gacaaaataa gcagtttttt
     8521 tgaataattc aggttggctg ttttgcagcc acttgatttt gttcaatggt gtcattggat
     8581 gaatcggcgt cccagtctgc cgataaaatt ctttgccaag cacggtatat ttcagttttt
     8641 ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat tcgagataac ggtgttttct
     8701 cttcatccaa caatatcagg ccatgcatcg cagaggagaa ggagacaaat tgaatgattg
     8761 tgtctgaagg caactgttta attacttctt ttataacggt gcagacacct tgaaagatca
     8821 attcctgatc ttgttctgcc atcccttgtg tttcttggat caaagggtag cgaaaataag
     8881 cggatgctgc ttcttgggct tcatctgtga aagcaactgc tttggtttgt gtggttccga
     8941 tatctaatcc gattgaaacg ttcatttttt cacctcaaat tttatgtatg tacaaaaatt
     9001 tagtttaagc ttgcgtatct tggttttgaa aagggggaga tggcgtgtta ctttatttta
     9061 tcattggttg ctgtatcgga tcatttttat gtttaaccgc ccagcggatt ccgttaggtc
     9121 actctattat ttatcctaga tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg
     9181 aattgattcc gattctctcg atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc
     9241 ggttgccctt ctactatttg ttggcagaag ccttatgtgg tggtcttttt gcctggttct
     9301 ttactttttc ttccgcaaga aatttttcga cgttcatatg gatgctttcg gctttgctat
     9361 tttcgttgat ggatttattt tacttcatgg tccattctca cacgctattc tgttcttggg
     9421 caattttatg gattttttgg ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa
     9481 cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc ctatctcgga gctggcgata
     9541 ctctgttgat gctttcttgg agtggtggct tgtctctaga agagttgctt cagatcttgt
     9601 ttcttgctag tctgtttgga atatccgttt tttctgttta ctttatctgt cataaaaaaa
     9661 agttggaaaa gctgcctttc attccttttt tgagcagtgc gttgctgatt gtccttcatc
     9721 ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca acctctgata tatagtgttc
     9781 tcttaccaaa tccttgtgac ttggagctag gcgacaggtt cacaacctct ccactaaatg
     9841 gtgttcaatc agctgaccct cagtattagc tcaaaatgct ggttttacaa cctctgatat
     9901 acggtgttcc ataagtagct ttttcggctt cttatttctt gacgccccaa tgtcgacgaa
     9961 ggaaacctgc acgtccagaa ccttctcgat aaagattgta atggagcttg tttttcttgt
    10021 aataatcctg atggtaatct tctgcaggat aaaattcgct tcttggttcg atttttgtca
    10081 cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat
    10141 ttcgttcttc ttcatcatta tagtagatca ctggacgata agaatcaccg cgatcagcga
    10201 actgtcctaa tgcgtcagtc ggatcggttt gctgccaata gatttccacc aactgatcat
    10261 aagaaataac ttctggatca tattcgatct ctactgcttc tgtatgccct gttgtgcctg
    10321 ttgttacttg ttcataagtt ggatttggta catgcccgcc agtataaccg gatgtcacag
    10381 aaatgatccc tggctgctct tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga
    10441 aaactgcttt ttttgtcata taaaatcccc cttttttctc atcatacctt gatttccaaa
    10501 aggaggataa cgttttgctt gtgacagaaa aaacggaaac tattatatac gtgatacggc
    10561 ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg gttaaatcag gaatcacata
    10621 ttcagcagaa ggaagattta tgttttcacc tattcccaat acatgcatgt taccagccaa
    10681 agctgcttga caaccagctt ctgaatcttc aatgaccaag catgcatttg gcggtacatt
    10741 caattgctgt gctcctttga gaaaaacttc tggatcaggt tttgcttttg atacttgcgt
    10801 gccgtcaata aggacatcaa aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc
    10861 atttttgctt gctgatccca atccgatctt gatgtttgtt tttttcaagt actcaagaac
    10921 agctattgca ccaggcaaaa cggatgtttc atccattttt tgaatcgctt gaagatatag
    10981 attgtttttt tgctcagcaa aggctgattt ctctttttct gagaatttct gttccatttt
    11041 tccaaatgac agcaatcgct ctagtgactc ggttctggat attcctttca gttgctcgtt
    11101 ttctttctca gtgaatggaa tggacagctg gttagctaaa accagccacg cttcgtaatg
    11161 ataacgagct gtgtctacaa gaacgccatc taaatcgaat agtgctgctt tgattttcat
    11221 cgattacctc cttgaaaatg aacgacttga agtaaagatg cgatgttttt agctgtgcga
    11281 tgaagattat cagtggcttg ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc
    11341 ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc ttcctttacc aacacttccg
    11401 caaatagcta tcacaggaac gccctctggt actcttctag caatcccaac tggcgctttt
    11461 ccttttagac tttgtccatc tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt
    11521 acccgtttat cgaactccag taaatccaat acgtagtcga tgccagagac tattttccct
    11581 gaagcaaatg ctaacagtcc tgcacccatt ccaccaccag cactactgcc cgatgcctcg
    11641 attacttgag gggcaaatct catataaaaa tcatgcatgg cttggtcagc cactgctaat
    11701 tcattttctt tcaagccttt ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat
    11761 aaaagattag taacatcagt aatgacagtg attttggctt taatcagctc aataggcacg
    11821 ttcgttttat caactgtagc aattttacct agattttctc ccagtgcttc tactttttga
    11881 ttatcttgat cgtaaaaagc ataacctaga ccttctgcca tgccgatgcc gccatcgttg
    11941 gtactacttc cgccaacgcc gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt
    12001 atcagttctc ctactcctcg cgtggataac ctaagtggct gttgttggtt tagtgggaca
    12061 tctttcaatc cgacgatttc tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac
    12121 tggatttctt tgtttttgcc aaatgcatta gtaacgactg ctgtttgccg cttcaacctt
    12181 aaactgtctg ctaatgatga aaccgttcct tctcctccat cgcctatagg tagcagatca
    12241 tattcagcct gaacaaaaac ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag
    12301 gctgtcagac tttctttgaa agaatctggc gcaatcagaa tacgcattat tttcccctcc
    12361 gttccaagat cagttctttt tccagctggt attggtctcc attgacaaaa ataggtaatg
    12421 tttctttcgt ctcttttttt attactattt gtttttttgt caaaacaaat agcaacctgt
    12481 gatttttcca aataaaagga aaagaaattt tttcccatgc tgaaggaagt cttggagaaa
    12541 tagaaagagt tccattcgtt actgtaatac ctgaaaaacc aaatataact gctagccata
    12601 gtgctcctaa tgaagcagca tgaattccgt catctgatga atgcggatct ggtcctaaat
    12661 caatcaggca cgcttcttgg aataaatgat acgcccattc catttcttgg atacggcaag
    12721 cctctatggc gtgaatcgct ttactcaacg atgagtcgtg gattgttttg ttttcatagt
    12781 aatgcaaatt cttagaaacg attggttgag aaaaatactc aggtaataag tataacagca
    12841 tgacaagatc tgcttgcttg ctgacttgga gttcatttac ttcgctacgg gcataatcaa
    12901 gtaaaataga ctggctgcca ggacgttctt tgtaagtggt caaatcaata actggcttac
    12961 tgagatacgt gtcatcttgc ggaatcagct gttctttgtt aggcactggc aaatataact
    13021 tatctaaaaa attttgacat tctttcaaaa aaagcggatc gccagcttga tatttaacag
    13081 ctaaggccac attataatgc gccagatagt tcgtatatgc attattgtcg atatattctg
    13141 tgtattcatc tggaccaatg actctgtgta tctctaaatg atcatcgact tttacagctc
    13201 tgctcagcca aaaagctgct gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg
    13261 attgatcctc tgttgcttgg acatactctt gaacggcata agcaaggtct gcactgatat
    13321 gatgttctgc ggcagctgaa gcaacttctt gtcttgttcc ggttttgata ttcaatgcag
    13381 caaatttggg tgtctcttcc tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt
    13441 gatagtgata atctcttgct ttttgccgtg cgccttctaa tcgatcataa cggtacagca
    13501 gcaaattctt tgctacttca ggaaactgat ataaaaagaa aggcaggata aaaatttccg
    13561 tatcccagaa aacatgccct ttataacctt cgccagttag tcccttcgcc cctacactaa
    13621 cacttggatc attgcgggga gtcataatct gtaaatggta agcagcgaag tccatcgcta
    13681 actgatcgaa agaagaatcg ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc
    13741 aataagacat cgactgctct aaaatttctg tataactaac gcttttctgg gcttgggcaa
    13801 tacttttctt aaacgggtct gtttgctgaa gagaggtaga gagcacgatg atcttctcca
    13861 gcgtaaagga cgtatttttt ctgatctttt ctgaaaagga agcggtgatc cggcgatttt
    13921 ttgcttggaa tacacctgct ttattgaatt tagctgcaat cccaatatca attttacttt
    13981 ctgttgtctg gtaggatgca ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa
    14041 ccaactgctg tgtaccaaaa ttggtttgct gtccatcgat acccgttttc acatctactt
    14101 ggcagtcttc ggtcaacggc ttgatgacca ctttcagggc tgcctgatgg agattatttt
    14161 gatcagcaac acgacaagta atgacttcca ctttttttcc ttgagcagtt tcaatgagcg
    14221 catagcgttt aagctctccc atttctaaat ctagtgcccg ctcgtatgca agaactttca
    14281 tcgtctcaag agaaaaatgt tcaccgtcga tttttatttc ccatctgatg acatctggga
    14341 gattcactaa ctcggctgat tctgctccta aaggtcggtt atatacacca gctgcaaaca
    14401 ttccccttac ttgattccga tagtcttcct cctggcttcc tctgatacca aaaaatccat
    14461 ttccaatggt catctgactc gcatacttat tgatatactc taaatcaaac tgtttttctt
    14521 ctactttcat aaggtaataa cttctcctgt ttctgccgaa cgatataaat tttccaccag
    14581 attctggatg acatatcctt gccagccatc tgcaatcatc tgagtctggt caccgagcgc
    14641 ccgattcaca aaatgagcca tacttagttc atgtgggtca ttaactggcg tggtcttttg
    14701 taaggaaacc agctttccat ctacatcttc gaatagttcg cctggataca gattagcacc
    14761 tgcctgatcg ccacaaaaag ccacattcag tttgtcttct ctgatattca aagcaaacga
    14821 agtattcagt tggatgatct tgccgttttc taattccagt gtgccaaaaa ttgcatcttc
    14881 tactgtgaat ttgcttggat cccatttccc gaatttccct gaattttttt tcgtaccaat
    14941 tttttggaag ctgtacgccc gaacgctttt tactttagga aatccagtaa tatacatcgc
    15001 tgtatcaagc atgtgaatgc cataatcgat caaaggcccg cctccttgaa gttctttgtc
    15061 gataaacgtt ccccagcctg gaacaccaga ccgacgaagc gcattcgctt ctacgtaata
    15121 aatatccccg agaacatcaa gatgattctt gatcattgtt gcttcgtcag aaaaacgatg
    15181 ctggaatcca taagccaaaa tctgcccgtt tttatcggct gtctctgcca tctttcttgc
    15241 ttgatcagct gtcatagctg gtggtttctc gcacatcact gaagcattag cttcgagggc
    15301 agccaataca tgttcgaagt gaaagcgatt gggtgtacag acactcacga tatcgggaga
    15361 aatctcattc agcatttgtg taactgatgt atacgcatga gaaaatcctg tacgtcggca
    15421 aaagtcttct gctcgctggc tgactggatc aaccacagcg gccaattcta agacatctga
    15481 tcttttttga taataagcag catgtacttt ttctgctact tgtccagcgc ctatgattgc
    15541 tactttcttc atttatttct ctctcctttg cttatttact tattgttatt ctttctctat
    15601 tctttctcta aatcatttaa atgacgcgct gtttccagac agtttttcaa gtattgcaat
    15661 gattcctgat aaattttcac ttcattttct cctcgcaaac gacattcata taccatataa
    15721 ccttcatact cattttgaag tagcgtttga aaatggcgag taaaatcaat tgcgccagat
    15781 cctggttggt agcgatgatt atctgctaaa tggatatgac ccacatactc tttttgcgta
    15841 gtcaatgctt ttgaaatatc atcttcttca atattcatat gataaaaatc agcgatgatc
    15901 tgcacatgtt tcaattgatt agagtcaatg taatgacgcg catccgctaa caaattgatc
    15961 atatggtctt ggtatcggtt caaaggctcc aaataaataa ccgtacctgt ttcttcggct
    16021 actttatcta aataacgaag tgattcgctg actgctttat agtctccttc tttgcttcgg
    16081 ggtgaaacca tcggcggtaa tcgataagtg aacattcccc aagctgctgg gataacgatt
    16141 cctttgcctc caacctcgct tagtgcacgc aaaatctcag caatttgacg tacaccatta
    16201 agacgccgtt cttcgataaa gtcaccgatc cacccatcgt agccgttaca agctgtcact
    16261 actgggatcc ctgtttcttt gattgcctgt ttgactgtgt cgatattttc tattaacaac
    16321 ttaccgtcga tttcgtattc ttcaaatcca cagtctttga taaatctaaa tttttctaaa
    16381 atgtctcttg ggaaaaatgc ttgatcttgc gtaccgaatt tcattgttct cctcctcgga
    16441 cttgaacgcc cattttgata ctttcttctg ggtgctggtc tacataacgc atgtaagctt
    16501 ctgcgctttc tgcaaatggc acaactggat caatgatttc ttcacaatcc aagtaaccgt
    16561 tcatcaataa ttcccaacat gtgtcctcga ttcgtttacg gttccatctt gggtaatctg
    16621 ggtttggttc actagatgct cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc
    16681 gaccaagatt aaacccagct ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc
    16741 catagccgat ccctattaaa gaagcttgta atgcggcagc actgccactc gtttcaataa
    16801 ctgaatctac tccacgttta tctgtcaatt cctttagacg aaaaccgaca tctgctttca
    16861 taggatctag cgtgtaatcg gcaccgtgtt tttctgctaa ttctctacgc tgcgctatgg
    16921 gatcgatccc aacgatggta gtagcacctg cttttttagc taattgaatc gcaatttgcc
    16981 cgatagcgcc taaaccgaag attgctacag catcacctgc tcgaacatgt gcttcccgga
    17041 taccacctaa agcgaattga gcaggatcgt aacatactgc atttttccat tgatccaacc
    17101 gatcaagttt acgtagttta taattatcaa caccgtttgt aatcagcgtt tcacgaatgg
    17161 gtccataagt gcagactaac tctccttttt gatactctgt caccaaactg cccgtttctt
    17221 cgatcgtacc gacaatcata tttcctaatt gaaattcgcc aaataccaca ccgcgttggc
    17281 ttccttcttc tcttggcaag aataggcgcc attcttctga aaattcttca tcgataaatg
    17341 gacttaatcc tctaaagtcg acaatttctg ttcctggttt tggagtagcg aaaattactc
    17401 ggatacgtac ttcgttcttt tcgatctcac ggtcttcgta atctttgaat ccggctattc
    17461 ttggttctaa tgcaactaat tttttcatct tttctttcct ctttcttttc tttttatcct
    17521 tttacgccgc cttcgactaa atcaccttta ataaatcgtt cagataacgc atacataatg
    17581 atgactggta aagcggtcac caatgaagcg gccatcattc gtccccaaat gtagtctggt
    17641 gtactgaata atgtgttcaa accgattggt aaagtaaatt tttcggaggt cgacaggaaa
    17701 atcgaagcaa ataaataatc attccacgaa accataaagc aataaacgaa gacagaaatg
    17761 attccagaaa tagccaatgg tataatgatc cgaataatga tctggatccg gctaagacca
    17821 tcgatcattg ctgcttcttc aatatcagtc ggaatcgtgt caaaataact ttttagcatg
    17881 aatatcgcag taggaagtgt ttgaacgacc atcgtgataa tcagtgctgt attggtatca
    17941 tacaaaccta gaccggaaat gattttaaat aatggaacaa cgatcaaaat accggaaaac
    18001 atataaacgg tgtaaaagct agcattgatt ttcgagcgag ctttgaagcg tagttttgac
    18061 aaagcgtatg ccccgctgat cccaatcact actgcagtta cggaggctat caaggagatc
    18121 actaaactat ttttgaaata catgatgaat ggaaaaatct cagcattgaa aatatcctga
    18181 taatgttcaa aagtccattc tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt
    18241 gaatccttga atgatgtcat cagcatgatc aaaaatggaa acaaattgac agctaatagc
    18301 aacaaaatcg tcgcgtaaaa taagattttc ctgctctttt tatttttcat ttcaagtcca
    18361 ccttctttcg tgagagcaag acgaccgctg caataatgat gaacaataga atcgatatag
    18421 ctgctgcttt tccaagatca ttgaacgcaa aggctgtctg atacaaataa acgcctaaga
    18481 tattgacttt attcgtcagt agatagacat ctgtaaacat atagaacatc cagatagatc
    18541 gtaaaacaat cacagttgct agtactggtt tgattgccgg cagtgtcacg atcttgaact
    18601 tctgccaagc atttgcaccg tccatttcag ctgcttcata aagcgttttg tcgactgttt
    18661 gcaaaatagc caaaaatgaa ataaacgcat acggatagta tctccagata gcaaacaaaa
    18721 cgaccataaa gaagctagca actggctggt cgaaccacag cggtgcttcg ttgaaaagct
    18781 gcaacctatc tactaacaga tcattgacga ttccataact attattgaac atatatttcc
    18841 aagcaaaaat caaagaaata gatggcgtaa cataacttaa taaaatcagt gaacgcaccg
    18901 ttttcctaaa tctgaacttg cggttaagca aaatagctac ggctaatcct aaagctgtac
    18961 tgcctgctac aactaagaat gtgtaagcaa ctgttaaacc taaagaacga taaaattcac
    19021 tatcacttaa aatttcaaga taattgctta atccgataaa aacggcgtct gagttaggat
    19081 tcagcggcat atcgaaaaaa ctgatctgaa tattggaaag cattggaatc agcactaaaa
    19141 cagctaataa aagcatgctt ggtgctaaaa gtcccatggc taattttaag tcagaatgtt
    19201 tccttggacg attcattgaa acctccccct gataatctgc caatagctat ctactttttt
    19261 cgaacaccat tggcagaact tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt
    19321 gatcgacatc ttcgtttccg acagttactt ggttcactgc ttttccgatc acacctgagc
    19381 ttgtgatatc tcccattttc gtgaaattct tttccccgac tagtccaaag acttgtactt
    19441 catcaaaagc actagcaatc tcagatggca gctcgccaaa agacttgatg acttcatttt
    19501 ctttgtaacc atccagttcg actacttgtt tattcactgg ttgagcacca ccaggagaca
    19561 tcaagaccca cttttccatg tttttcggtt gagataagaa agctgtgaat gcttcagcag
    19621 cctctttttg tttctcgtct agaccagaag aaatcgtcaa tccagatact gtgccataga
    19681 cagcttgatc tttttgtgtt gggatagcaa aaccgatatt ttctgagttc cccgcttcat
    19741 aaacagaagg caaaatgtaa gttgaataga ttgccattgg tgctgttcca ttcatgaaag
    19801 catctttgat ttctgtcaca tcattagagc ctggcatcgt gtattgggat aagttttgat
    19861 aaaacgacaa tgcttctctc atcttttccg tatcgatcgt tacctcgcct tcatcatcca
    19921 aaacatttgc tccatttgac aaagcaaatt gagagaacgc ttgctcactc attgttcctt
    19981 cagcagtagg catagcaatt ccatattgtt tattctcttt atctgtgaaa tgttgagcaa
    20041 tttttaaaat atcatcccaa ttttctggtt ctgaaaaacc agcttctgat aatttttctt
    20101 tgtcatacca aataccttga acccagccgc taattggtgc agcaatatag ctttttccat
    20161 cttctgaacg aacaaggttt ttggctccgt cataataatt atcttctcca gcttcctcga
    20221 taacgttttg gactgccgtc tgatcaatca gttcatcttt atccatcact ttagcaaaat
    20281 cctgactgac ttccattact tctggtaatt taccggaacg agctaacgta acgatttttg
    20341 tattgaacgc tgattcttct accggcactt gcttgatttt gatgtctgga ttttcttttt
    20401 caaaatcagc aaccagttca ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca
    20461 tgaattgaat cgttgtctgt tgtgattcgt tagagctttg actttcacca caagcagtaa
    20521 gaagaaatcc agtagctagc agacaggtag taacaataat ttttttcatt tttttccacc
    20581 tcttatttta ttattttcag ccacatatat tcatacggct ggaatatcaa ctttcgttct
    20641 tgactgcaat ggataatttg tccagaaaac aggtctaggt aatctccttt tggtaaaaga
    20701 taacctgctg attgattgga aaaattatgg ataactaaga ctttctcttc cttcgcttcg
    20761 cgaataaaac tgataaaaga atctgctgag tccagtaagt aaatcaaact attcggatgg
    20821 aaggcttttt ctttttttcg aatagatagc atctgggtca attcaccata tactgctgtt
    20881 cttaatggat ctgtctgcaa ctctttctca atatctgcta acaaaaattt ttgccgattg
    20941 atttttcgat tctctcctgt tttttctacg cctttgtaat catttcgact tcccagcaca
    21001 ctttgaatat aaattgccgg cacgcctggt aacgttacca aaaatgaatg agcagctaaa
    21061 aagcgtttgc ttctccattc cgtcgtatct gttggagaac ttaacgcatc tagatacgta
    21121 gcgtttatct catatggact ggtcgttcca tctgggtttt tcttgtagtt tactcgggca
    21181 ccctctttct tcagctgttc cactaattca aggatcgttt tttccggcag aatacctcgt
    21241 accggattca atccgatacc gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc
    21301 gatgcgaaat cgatggtttt taaccattct ttcaacttac ttgtattacc cgaacgaatc
    21361 gcttgaaggg tcaaaggtgg tagcgaaaac tgatagacca tctgtgcttc attctcgcca
    21421 ttcccaaaat aactgatatt atctttgtgt ggcacatttg tctcagtgat caataccgtt
    21481 cctggagctg cttcatcagc gattgcacgg aataattgga tgattttatg cgtttctggc
    21541 aggtgtatac aagaagttcc tagttctttc cataaaaatc ccacagcgtc caagcgaata
    21601 aatgaagcgc ctttttctaa gtaaaacaaa agaatatcga tcattttcaa taaaacttct
    21661 ggatttttaa aattcaagtc tacttgatct tcgctaaaag tcgtccatac gtgtttttct
    21721 ttcccgtttt tgtcaatgaa cgttgtaagt aacgggctgg tccttggtct agtcacgctg
    21781 cttgtatctg tatccagtgg caaatcaata aaaaaatctt tgtacgtatc attcaagttt
    21841 aaatattcct gaaaccaagt actttcggca gacatatgat tgcagacaaa gtcgaacatt
    21901 aaccgtgttt cgcttctcat ttcttctata tcttcccatg tgcccaaacg gtcgttgatt
    21961 gcctgataat ttacaacgga gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa
    22021 tgtacaactc taaatgtatc tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa
    22081 gttggttgct gctcttcaaa aaattgatcc ccataagtaa tcaagaaaat atcttcttcg
    22141 tcccaatgca gtttgcctgc ctggcctttc ttctgctgat aaacactgac tttttgttca
    22201 atcatagagg cggttttctc agacaaatct gagtcgcgat aaatcgcttg taaaagtttt
    22261 gtatacatcg gattcccctt tcttttttgg gagcgttccc aacgatttga tcatattatg
    22321 gaaaacgctt actgtcaata gcattttttt aaataatttt gatatacaca aaaatacaaa
    22381 taagaaagct tatgtatcaa taattaatag cttcttaaaa aaatatggtc tctcatgatc
    22441 atgcaagcta ttcctaaatt ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg
    22501 gaattattct aaaacgctcc caaaaaggag gtgcatataa aattgaaacc tactatttat
    22561 gacattgcga aaaaagctaa tgtctcaaaa tcaactgtct ccagagtatt aaataaccag
    22621 ccaaatatct ctgataaagc aaaagcaagt gtacttcaag caatcaaaga attgaattat
    22681 gaaccgagca aaattgcaag aggactgtca ggaaatggtt ttgacgcgat tctagcatta
    22741 tctcatcgta cttctcatac gacctatggc aatccttttt tttcagatgt tattcaatcc
    22801 atttctgccg tttctgaaca aaataacttc gacttgattc tacaaacggc aaaaacacca
    22861 gaagaagagc tagaaaaatg cgcgaaaaag atccaagaaa aaatgatccg aggaattatt
    22921 attttaagct ctacgatgga tgaaacactt ctttttgaat tggatcaata caatattcct
    22981 attgtggtaa tcggaaaagt cggaaacgac tacacgaaca tctactcagt ggacacggat
    23041 aatttccagg atagttacga tttggtggaa acactgatca agaatggaca ccgtcatatc
    23101 ggctgtctcc atgctccgac agaagtttat gtatccaatg atcgtctcaa tggttaccgt
    23161 caatgcttat ttgatcacca cctagatatc cgtaatgaat ggattataga cggcggcttt
    23221 cgcttagaag atagcatggc agcaatgaat gatttaatga agctacgaga aagaccaact
    23281 gcgatttttt ctaccgatgc attaaaatca ttgagtattt acaaagtggc agaacaatct
    23341 ggaaagcaga tacctgatga tctttcgatc gcaggattca gtgatccctt attgtcctcc
    23401 ctattttcgc cacagttgac tcggatagac attccgacaa atcagttagg aatcaaagcc
    23461 acagaacttt tattccaact aatccaccac caataccctg aagaaaaaag aatcattctt
    23521 tcaaccgaga ttactctcac agattcgatc caacaacaag aaatgtagaa cagaattata
    23581 aaagaacaga aacactgagg ataagtcagt tactctgttc ttttttcaat cactcatccc
    23641 tcgttacccc ctcccggtat tttagcgaat ttttcttatt ttccatctgt tttagatagc
    23701 tgtggataac tataatgttt gtgttaaact attttttcca cagggaagaa aaaactattt
    23761 ttaaccacgt tgtgtataag tttttatagt tcgttttttt aaacacattt acccttattt
    23821 caatccatgt tatttgggga tacttatctc taaaacataa atagaataac agacatgtct
    23881 actggttttg ttttattcga ggaattattc aataatagct agcaaaatat taaaaataac
    23941 cgctctatct tcccatcaat ttattttgca ttcaaaaaat gatctatctt tacttttgtt
    24001 gattgacttc tcttggaaga tgatcgttcc gataccttaa ttggatacga accgatgttg
    24061 ttaagatcct ttcccacttt tcttctagtt agacaaatta ttttagacaa accaaaaaag
    24121 aggttgtgac aataatttgt cacaacct
//
LOCUS       DAHEYX010000016        24070 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHEYX010000016 DAHEYX010000000
VERSION     DAHEYX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.016"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(111..557)
                     /locus_tag="MB219_000448"
     CDS             complement(111..557)
                     /locus_tag="MB219_000448"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCC8426185.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(568..2781)
                     /locus_tag="MB219_000449"
     CDS             complement(568..2781)
                     /locus_tag="MB219_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCC8426186.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(3014..3778)
                     /locus_tag="MB219_000450"
     CDS             complement(3014..3778)
                     /locus_tag="MB219_000450"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCC8426187.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3779..4726)
                     /gene="prmA"
                     /locus_tag="MB219_000451"
     CDS             complement(3779..4726)
                     /gene="prmA"
                     /locus_tag="MB219_000451"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCC8426188.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4739..5227)
                     /locus_tag="MB219_000452"
     CDS             complement(4739..5227)
                     /locus_tag="MB219_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCC8426189.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5193..5864)
                     /locus_tag="MB219_000453"
     CDS             complement(5193..5864)
                     /locus_tag="MB219_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCC8426190.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6002..7288
                     /locus_tag="MB219_000454"
     CDS             6002..7288
                     /locus_tag="MB219_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCC8426191.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7360..7554
                     /gene="ssrS"
                     /locus_tag="MB219_000455"
     ncRNA           7360..7554
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MB219_000455"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7632..7916
                     /locus_tag="MB219_000456"
     CDS             7632..7916
                     /locus_tag="MB219_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426192.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7989..8468
                     /locus_tag="MB219_000457"
     CDS             7989..8468
                     /locus_tag="MB219_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCC8426193.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8691..8834
                     /locus_tag="MB219_000458"
     CDS             8691..8834
                     /locus_tag="MB219_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCC8426194.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9412..10995
                     /locus_tag="MB219_000459"
     CDS             9412..10995
                     /locus_tag="MB219_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCC8426195.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPINHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11432..12616)
                     /locus_tag="MB219_000460"
     CDS             complement(11432..12616)
                     /locus_tag="MB219_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCC8426196.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12641..13648)
                     /locus_tag="MB219_000461"
     CDS             complement(12641..13648)
                     /locus_tag="MB219_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCC8426197.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13820..14179)
                     /locus_tag="MB219_000462"
     CDS             complement(13820..14179)
                     /locus_tag="MB219_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426198.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14151..14522)
                     /locus_tag="MB219_000463"
     CDS             complement(14151..14522)
                     /locus_tag="MB219_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCC8426199.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14539..14841)
                     /locus_tag="MB219_000464"
     CDS             complement(14539..14841)
                     /locus_tag="MB219_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426200.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14858..15328)
                     /locus_tag="MB219_000465"
     CDS             complement(14858..15328)
                     /locus_tag="MB219_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCC8426201.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15303..15581)
                     /locus_tag="MB219_000466"
     CDS             complement(15303..15581)
                     /locus_tag="MB219_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCC8426202.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15578..16627)
                     /locus_tag="MB219_000467"
     CDS             complement(15578..16627)
                     /locus_tag="MB219_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCC8426203.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16584..17513)
                     /gene="tadA"
                     /locus_tag="MB219_000468"
     CDS             complement(16584..17513)
                     /gene="tadA"
                     /locus_tag="MB219_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCC8426204.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17645..19399
                     /gene="ade"
                     /locus_tag="MB219_000469"
     CDS             17645..19399
                     /gene="ade"
                     /locus_tag="MB219_000469"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCC8426205.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19497..20159)
                     /gene="trmB"
                     /locus_tag="MB219_000470"
     CDS             complement(19497..20159)
                     /gene="trmB"
                     /locus_tag="MB219_000470"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCC8426206.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20229..21011)
                     /locus_tag="MB219_000471"
     CDS             complement(20229..21011)
                     /locus_tag="MB219_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCC8426207.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21215..22426)
                     /locus_tag="MB219_000472"
     CDS             complement(21215..22426)
                     /locus_tag="MB219_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426208.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22419..23156)
                     /locus_tag="MB219_000473"
     CDS             complement(22419..23156)
                     /locus_tag="MB219_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426209.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23294..23728
                     /locus_tag="MB219_000474"
     CDS             23294..23728
                     /locus_tag="MB219_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCC8426210.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23721..24065
                     /locus_tag="MB219_000475"
     CDS             23721..24065
                     /locus_tag="MB219_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCC8426211.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         7156 a         5078 c         4295 g         7541 t
ORIGIN      
        1 gctgacaaaa gaagcgcttt ttgcagacaa aaaagccact taagttagag tttatcagtc
       61 taacttaagc gacgtgtttc atagttttta ctagtgaaaa ccagattact ttatttgttt
      121 ttagaatcaa taatgatcgt tacaggtcca tcattgacga gagaaaccga catatctgct
      181 ccgaattgtc cggtagcgac tgtgatgccg cgacttctga ttccttcatt gaatgattcg
      241 tataaaggaa tagctgtttc tggtcgtgcg gcttccacaa agcttggtcg atttcctttt
      301 ttcgtatccg caaacagtgt gaattgagag atacttaaaa tttgtcctcc cacatcttct
      361 acactaatat tcatttttcc ttgttcatct tcaaaaatac gcatttgtgc aatttttttt
      421 accagatagt ccacatcttt ttgcgtatcg gtttcatgaa tgcctaataa aacaagtagg
      481 ccgtagccaa tttttccaat ttcttgctga tcgactgaca cagcagcttt acttacccgt
      541 tgaacaactg ctctcatatc tgtcctccta tccattcgtc cggcgtacac tgtagacatc
      601 aggtactgct ttgattttat ccacgatact ctttaggtgg gataaattct ggataccaac
      661 cgtcaaatgg atcgtcgcca ttttatcttt attcgtccgt gcttcaacgc tgttgagatt
      721 tttcgttagc gcattgactg tttgaagtac atcattcaat aagcctgaac gattatagcc
      781 gtaaatttcc aaatctgcat catactcttt tcgtgtattc gatgtatctt cccattcgac
      841 ttcaatcaaa cgttctgcta catttggttt gtcaggctga acattcggac aatctcgacg
      901 atgaatggat atccctctgc ctttagtgat ataaccaacg atatcatcac caggaatcgg
      961 attacagcag cgactgatac gaattagtaa gttttctacc ccttgaatga caacgccacc
     1021 ttcatgacgt actttcattt tgtcaggttc ttttttcggc tgattcatga tttcttctgc
     1081 ttcttgcttt tgctgctcga ttttttgttc tttacgttct ttttcagtca gacgattggc
     1141 catcgtcaag gggctaactt ctccataccc tacagctgca tagagatcat cttctgtttg
     1201 ataattaaaa cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat ctttaggcgt
     1261 aaatcctaga tctgtaatac atttgaccac ggattcgtgg cctttgatca cattttcttc
     1321 tcgatcttgg gctttgaaga aacgtttgat cttatttctt gctttgctag tagcaactaa
     1381 tttcaaccag tcgcgacttg ggccaaatga atttggagaa gtcatgatct caatgatatc
     1441 tccgtttttc aatttgtaat caagctgcac cattttgcca tttacttttg caccagtggt
     1501 tttgttaccg atatctgtat ggatactgta tgcaaagtcc aatggtccgg atccttttgg
     1561 taactcagta acgtctcctt ttggtgtgaa gacgtagact ttatcactaa agatatctcc
     1621 ttttacgcct tccataaatt cagaagcatc atagctttcg tcttgaagtt cgagtatctc
     1681 atggaaccag cttaattgtt tcgtcatacc atctggttct actttttcat tttttccttc
     1741 tttataggcc cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt cctgtgttcg
     1801 gatttgaatt tctaccggat ttcctgccgg accgatcacg gtagtatgca aagattggta
     1861 catattagct tttggcatgg caatataatc cttaaatctg ccgggcattg gtttccattt
     1921 tgtgtggatt gctcctaata cagcataaca atccttgatt gaatctacga tcactcgaat
     1981 agctagcaag tcataaattt cgttgaactg ctttttctga tctttcattt tacgatagat
     2041 tgaataaatg tgttttggcc gtccatagat ttctgcaaaa atccccagct cttctgtcgc
     2101 tattcgaata tcttcaacag taccacttac gtatttttca cgttcttctc tcttcgtctg
     2161 catcaagtgg acgatacggt aatactgttt tggatttaga taacgaagtg ctgtatcttc
     2221 taattcccat ttgatccggc tgatccctag acgatgtgca agaggcgcat agatttctaa
     2281 agtttcctga gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg tacgcatgtt
     2341 atgtagtcta tcagctaatt tgaccatgat aactcgtaaa tcttgagcca ttgctagcag
     2401 catctttcgg tggttttctg ctagctgctc ttcgtgggat ttatatttta ttttccctaa
     2461 tttggttacg ccgtccacta acatagcaac gtcatcgcca aattcttctc tcagatcttc
     2521 taaagtgatt tctgtatctt caacaacatc atgtaagaag cctgtagcca ctgtatgggg
     2581 atccattttt aaatctgcta atatacctgc gacttggata ggatgaataa tgtagggttc
     2641 gcctgatttt ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg ctttctcaac
     2701 aaatgctaca tgttcgggac ccatgtattg actaacgagc tttatgacac cgggtcccgt
     2761 taaaatttct tcttttgtca caggaatcga tccccctttc ttttttcggt aaaacgggaa
     2821 tcgggacaaa tatctgtttg ataattgtcc caaaatccgt gcagttatac gtttttttac
     2881 ggaaatgttc gctttttaaa agctttttca aacgattttt cgtatccatt gtataaataa
     2941 ttgtttctat tatacaaatt gttgactatt ttttcaattc tttctgtcga tactctttta
     3001 ttaatgaaga acgttagctg aaagaaggat tcattaattc ccattgatag ctagcagcac
     3061 ttaataaata aagaggtgct gtttctgtac gaaggattct tggtcctaat ccacaaagta
     3121 tccctttgtg ttctacaaaa taatcgactt catcgcggga taatccacct tctggtccaa
     3181 agacagctaa gattcgacta tctggttttg tttcactaag aattttggct gcatttgatt
     3241 tttcgccttg ctttgctgat tcctcataag caaccaagac cacgtcataa tcatctagcc
     3301 gctcaaataa atcttgtttc gtttccaata actctactga tggcacgatc tgccgatggg
     3361 attgttccgc tgcctcttga gcaatctttt caagccgttg ttgtcgtttc atacgttttt
     3421 ttgcatccca tttaacgaca gaggctgatg ccgggaaacc aagaaaagct tgcgctccta
     3481 attcagtgga tttctgaacg atccaatcca gcttttcccc tttaggatag ccactcgcta
     3541 tggtaatcga acttggcagt tccttttgat tttcttcttt tttaatttct ttcagataaa
     3601 cagcctcttc agtaatctcc gtgatttctg caacaatggc tatttgatca ttgaatgcca
     3661 aaaaaacttc atcctcgggt tccatcctca taacccgtac aatgtggtga tagttttccc
     3721 cttctaaaac aaaatagtct tttgattcat atgattcttt taaaaaatac cgctgcatct
     3781 attccacctc tggttttttc aaaataattg cataccagtc tttttgctgg aaaatttgat
     3841 ccacgataaa cccttgttct aacatggcat ccagaaccat ttgcttcttc tcttcaatga
     3901 ttcctgaaac gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct gggatcatca
     3961 ataagatgat atctgctaaa atgttcgcaa cgattacatc actttcaatc tcgattcctg
     4021 tcaaaagatc gttagctgat acatggacat ctttggctat ttcattcata tccatatttt
     4081 ctttcgccga acgaacggct acttcgtcta aatcaaaggc atagacatct tttgcaccaa
     4141 gatattttgc tgcaatactt agcacgcccg acccagttcc cacatctagc aatgtttcac
     4201 caccacgcaa gactgtttct agtgctgtca atgtcaagga agtcgtggga tgagtgcctg
     4261 tgccaaatgc cataccagga tctagtgtga taattttttc tagaggatct tctgtctggt
     4321 agttttccca gcttggcgca accgtcagca ttcgcgtaat acgcaccgga tgatagtatt
     4381 tcttccaggc tgttgcccag ttgctttcct ctacttcact aactgttact ttattttttc
     4441 caatagctag accaaactca ggtaattgtg tgatacgatc tttgataaaa ggcaagattt
     4501 ccggtaagaa agtcgtttcc ggaaaataag ccatgactag tgcgccttct ttcagcgatg
     4561 taaaatcatt tttatctaaa atctcaccat attgatccga ctgaaaatgt tcaacatcta
     4621 aagaatcttc aatcgcgaca ccactcgccc ctgcctccat caaaatatta gcaactgctt
     4681 cgactgcttc actttcagtt tctactttta cttcattcca tttcatatct gtcactcctt
     4741 aataacttgg gtacggtaaa taagtatctg cttcattttg ctgttttact tctactaatt
     4801 ctgctagttg ctgattatcc cagtgaagct cgaatacagc ttcgtcatcg tcttcatcca
     4861 aaaaatcaag caaatcgctt tgcccttgag ctaatacttc atttagatat tccactaaag
     4921 aatcagctac tgattttgcc aacccttttt ttccttcata agggattact gctagataat
     4981 tgcgttcatc aaaaacagat ttctgaggat taaaaagaag tacgccgtct tcaaattcga
     5041 taattggttc ttgcgaaacg atcccttctg catcatcaat ctcaaattgt gctttatttt
     5101 ctgcaaacaa gcgaacaacg atctcgatcg tatgattttt tgtatcccaa tctaaagcta
     5161 catcatagtc tgtcacttgt ttttcaatta tttcatctaa atatgttaat agatccttct
     5221 ttttcatttt ctttcctcca accccaatct ttctcgattt ggttttttct ttgtaaagag
     5281 atataaggat ttcccgacac aacaaaacgc aacggctttt ctgtccatac acctttgttt
     5341 gggataccga ttctaggcaa agcattgatc ttttttggtg tctttttttt ctcaaaaact
     5401 aagcgtaacg aactatctgc tatagactga ccataaagtt cttgaggaat tgctaatgct
     5461 tccactaatt ttcccggacc gttgctgata tctggaccag tttttccgcc acgattttta
     5521 ctcatttgat caatcattgc ggcgggttcg attgcacgta tcattactcc ttgaggaatc
     5581 ccttctggct gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta aaggtagatc
     5641 gtacctggtt tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc atagctatgg
     5701 gcggcctcgt cttctggacc taggtatgct tccgcgtcta caatatatcc agctaatctt
     5761 ccttctggtg tgtcatgctc caaatacatc cccaataaat cccgtgccac ttctggcgtc
     5821 gttttattcc aaaaaaattc atctatcttt tgtttatccg tcatttttct cacctacttt
     5881 atcatacaca atattagttg gataagctat gaaaacaaaa caacagaagg ctgttctttc
     5941 taggcatctt gctaaatatg atataattaa gggacgtaaa aacaagaagg aggaaaatcc
     6001 tatgcaacag ccattggctt atcgcatgag acctcgaacg atcgatgaag tcgttggtca
     6061 gcagcatcta gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc gcatgctctc
     6121 ttccatgatt ctatatggac ctcctggtac tggaaaaacg agtatcgcaa gtgcgatcgc
     6181 cggctctact aattatgcat ttcgcatgct aaacgccgct acagattcaa aaaaggatct
     6241 acaagttgta gcggaagaag caaaaatgag tggtacagtg atcctcttat tggatgaagt
     6301 acaccgatta gataaaacaa aacaggattt tctgcttcct catttagaaa gtggccgaat
     6361 catcttgatc ggagcgacta ctgagaatcc ttatattacc atcaatccag cgatccgcag
     6421 ccgtacacag atctttgaag tcaaaccatt aacagaacaa gacatccagt tggcagtcga
     6481 acatgcttta agggataaag aacgcggcct tggacaacaa gcaatccagt tagacgaaga
     6541 tgctcttctt cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat tgaatgggct
     6601 ggaactggct acactatcga ctccttcaga taaagaagga aggattcatc ttacactgtc
     6661 gatcattgaa gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg gagatgctca
     6721 ttatgacgtt atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg atgctgcact
     6781 tcattatctc gcccgtttag ttgaggcagg tgatctggca agtatttgcc ggcgtctgat
     6841 ggttatcggt tatgaagata taggtttagg taatcctgct gctgcggctc gtacagtgaa
     6901 cgctgtatta gctgctgaaa aattgggatt gcctgaggca cgtatcccac tagcagacgt
     6961 cgtcgtcgat ctttgcttgt cgccaaaatc caattctgcc tacatggcat tagatgcagc
     7021 acttgctgat attcgagagg gcaaagctgg agatgttcct gatcatttga gagatagcca
     7081 ttacaaaggt gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc atcattttga
     7141 tcaggcttgg gtcaatcagc aatatttacc agataaactg aagaatgctc agtattatca
     7201 gccaaaagat acagggaaat atgaacaggc tctgggtcaa cagtattacc gtataaaaga
     7261 gtggaaaaag catcctccaa aaaactagac aagaaaacgg ttctgtttct gcgagctctc
     7321 tcattgctct ttttaaattc atatgctact atagaggtgg gaaatctgtg atgttcgaat
     7381 tgtcctcttt gtgtgttgac cgaacatttt tattgatatc ttgggacccg tcttgtgtca
     7441 ctgctggtaa catgccttat catttggtag ttcgaagcta cgaccatggg acccacctgc
     7501 ttgaattcgc gggatcaata ctacaggaca aacaacggca ttaacggaga ttaccatctt
     7561 ttaaagaata aagcaatttg ctcttggcaa attgcttttt ctatttgttg cttattttgg
     7621 aggatttgtt catgattagg attttaatga ttatcgccac tttagtatta ttattcgtta
     7681 gctattattt atttaataaa caggatatct tttttgtatt gatcaacaaa aatgataaaa
     7741 atcaaggatt cctacaattt tacggagctg catatgctgt tttaggcgta atgggtattt
     7801 taacagcttt tttcaatcaa cgctttattg ccttggtctt tttactgatc gtaattattg
     7861 tttctgccac cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag cagtaaataa
     7921 ttctcttttt ccctaaagtg ttctacaata aaagagtaca atacgattga cttgaggagt
     7981 gaatgcttat gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt tctgatgaag
     8041 ctgaattagc ttttaaaaag gcggtcaatg tagctatccg aaataacgga gaacttctat
     8101 tagcacatgt cattgacaca cgttctttcc aaaccgtttc atcctttgat ggaatgcttg
     8161 cagaacaagc tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc aatgcgaaga
     8221 aagctggctt gaataatgta acctctgtcg tagaatatgg ctcaccaaaa caaattattg
     8281 ccagagaaat cccagaagat aatcaagtag atttgattat gttaggagcc acaggattga
     8341 atgctgtaga acgtttattt attggttctg tttctgaata tgttattaga aacgcagctt
     8401 gcgatgtgct tgttgtccga actgatttag aaaaccaatt gccagctaaa gaccacacca
     8461 aagcttaaca cgtagacgac ctgccaacta tcggcaggtt tttttgcaca aaaaaccgtg
     8521 ataaaaacaa aagacgcggc aagcactcat cggtctttat aatgtttaaa ataaagggaa
     8581 aacagtcaat gagttcaagt aaaataaaaa aaagtgaaca ccagggcgat tagtcgccct
     8641 ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat gtggaagttt
     8701 ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat tttatcgcca
     8761 aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca ttcggtaaat
     8821 cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat actcctgaac
     8881 gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat taagcaaatg
     8941 gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt cgcctgaacg
     9001 ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag tcagataaaa
     9061 aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc gctagttgaa
     9121 ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg aaaatactat
     9181 gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata tcgcatttag
     9241 ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa cagttgaatt
     9301 cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag tttaaataac
     9361 acaggccatg gataaccatg gcctttttga gtggtctcaa ggagagagat catgatcata
     9421 caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga tttatcctgt
     9481 caaattgaag ctggtgaaaa aattgcctta gtcggtatca acggaactgg caaatcaacc
     9541 tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag tcgcaaaaaa
     9601 ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac cgtcaaccac
     9661 tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag tcgctacgaa
     9721 gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta cggtgagctg
     9781 cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc agcgatcttg
     9841 caaggcttag ggattccaca ccgtcaggaa gcacggttaa cagaacttag cggaggcgaa
     9901 cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact tttactcgat
     9961 gaaccgatca atcatttaga tcttaacagt attcgctggt tggaaaacta tttacagaat
    10021 acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca agtcaccaca
    10081 aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta cagccgtttt
    10141 cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact gcagcaaaaa
    10201 gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca tgaaggagac
    10261 aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa actgacactc
    10321 gtcaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg tggaaaatct
    10381 ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca agtactattc
    10441 aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg cgacaatggt
    10501 gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta cggtggcacc
    10561 atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca gtttgccgat
    10621 cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga agtcgctcga
    10681 cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat caaagattta
    10741 tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa aatcaacgtc
    10801 ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga aattgaagaa
    10861 atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta ctttttgcag
    10921 caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca gccgctcggc
    10981 caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata cctgtgcttt
    11041 cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc aatgaatgtt
    11101 tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc cacgaggctg
    11161 atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg aatgaaaata
    11221 aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc cttttgacca
    11281 cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat tgaagttaaa
    11341 ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct cttgcatgcg
    11401 tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc acatcacggg
    11461 caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat tcttttgtag
    11521 agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca caaccaaacc
    11581 aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg ataccagcag
    11641 taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg tattttcgga
    11701 tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag ttcttttcaa
    11761 gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc ttgttcaaaa
    11821 cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg agaacagctg
    11881 gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg aatcccatag
    11941 aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt cctaagtggc
    12001 aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca cgttccgaca
    12061 cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct tcataataag
    12121 acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa gatgtatcga
    12181 atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc cccattaagt
    12241 ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc attactttct
    12301 catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg tgccctactc
    12361 cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct aacagcattt
    12421 ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga ttatcgccgt
    12481 acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct ttcgcaacta
    12541 cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca gcattgattg
    12601 caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca gatgatttcc
    12661 agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat gaagcaagtt
    12721 tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa
    12781 tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc attccttgaa
    12841 gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt tctaaaaatc
    12901 cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt gattgctcta
    12961 gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac tcttttgctt
    13021 tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg acaggatcta
    13081 ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt gtcaaatcac
    13141 ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt ccttctgttt
    13201 ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc attcctgccg
    13261 caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca ataagaaaaa
    13321 caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct
    13381 ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa
    13441 gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt gtcggcactc
    13501 catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg acataggctt
    13561 ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg ttttgattaa
    13621 acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat attataacaa
    13681 ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt
    13741 tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca gaaaagaggg
    13801 cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt acttcttctg
    13861 tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt tgtacccact
    13921 gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag
    13981 actgttccga taaagccatc tgcttcatta tttttgctat ataataattt ttgattcgta
    14041 tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa aacgtacata
    14101 aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt ctactgatcg
    14161 ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata aaaagaaatt
    14221 gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg ttgatgtccg
    14281 ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata ctgtttatcc
    14341 tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct cttgagttct
    14401 tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg caaaaattca
    14461 cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact gctcaatatc
    14521 atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt tttcaatact
    14581 gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat tatcgacaat
    14641 ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat acatccgctg
    14701 cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac ttttcttttc
    14761 tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag aagcgatcat
    14821 aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt tttgtatagc
    14881 gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc caataaaatt
    14941 gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata gaagcatggc
    15001 gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta tctggattcg
    15061 gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt tggatctgat
    15121 tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg aaaaattgat
    15181 agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa aggatcgttg
    15241 taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct aaaaccttac
    15301 cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc attcaacaat
    15361 tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt ttgtgtgtct
    15421 accaactgga tgatcgcttc acgactttct tggtctacat gattcctata acgcgacaaa
    15481 ttagggacga acaacaacac taatacactg atgatcagta agacgattag catttcgatc
    15541 aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc tcctctatgc
    15601 ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc gctacgccca
    15661 aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa agctgttttc
    15721 ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc tctccctgta
    15781 aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc tcagccaatg
    15841 acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc agactttctt
    15901 gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca tgaaaaagct
    15961 ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt ccaatcaata
    16021 attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga cgatacctta
    16081 ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa tgaaagactt
    16141 gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata agctgaggca
    16201 aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat agcatcggat
    16261 atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac tgtttggcaa
    16321 tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc tgtaccagtt
    16381 gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct tcctttagct
    16441 gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt aataaataaa
    16501 gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt gcaaactggt
    16561 tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt ttcctgagct
    16621 tcttcataac tttcctccca tgattttttt actgattctt cctccataaa ttgataactc
    16681 cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat gacaccagct
    16741 aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac caatgtacct
    16801 tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc
    16861 gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc tggacgatgc
    16921 cgtaatgcga gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt gatctgtagc
    16981 tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac ttgcaatttt
    17041 tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg tccgctgaaa
    17101 aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg agaactaaag
    17161 caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact ctctcgttga
    17221 aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa agaataagta
    17281 aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa atacttaaat
    17341 cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt tcgttttttt
    17401 ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac gtcttgggca
    17461 tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc cattattctc
    17521 ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat gaatttacaa
    17581 aaaaatattg gcaaaaaccg aatttatgtt atacttataa aaatttatct tgggaggcac
    17641 tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa agcctgctga
    17701 cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga
    17761 tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca
    17821 taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc atatcgaaag
    17881 ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag ttactactgt
    17941 agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg agtttatgat
    18001 agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt gtgtacctgt
    18061 aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc cttttttaca
    18121 aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag caaataatga
    18181 aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa ttgacgggca
    18241 tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta ttcggacgga
    18301 tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta tgtatttaat
    18361 ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga tcacacctga
    18421 aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt tagtaaaaga
    18481 aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc cattacaagc
    18541 cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac taggtgcgat
    18601 tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc tgccaattca
    18661 tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag accatttatt
    18721 tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa
    18781 tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg aaattatccc
    18841 gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg aacgattcat
    18901 tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc atcaaacggg
    18961 aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga tagcgacaac
    19021 gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg atatgctgta
    19081 tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca aacagcagga
    19141 aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt tttcgatcgt
    19201 ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag ctaaaacctt
    19261 tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat tgaagatcac
    19321 cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga
    19381 tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc tgtaatgttc
    19441 attttctggg atttatttat ggatattaat gtttttatga aagcagaaag ttagtttcac
    19501 agacttttct ttttggtctt atcttgaaat tgagcttcta ctcgataaat acgttgacct
    19561 tttgaagaaa atttttcttc atactccgtc ataatattgc cttcaaattg actttgatgt
    19621 aaatctaacc atacctgttt gaggatcatg ccatattgag aaaaacttgc cagagaatac
    19681 tcaaacaatc cttgattatc tgttttgaaa tggatttctc catttggacg aagaatctgt
    19741 tcatctacag caagaaaagt cttatatgtc aatcgccgtt tttcatgacg tttttttggc
    19801 catggatcgg aaaaattcag atagatttga tctacttcgc tgtcagcgaa atactctgtc
    19861 aacgctgatc catccacatg tagaagtttc aggttaggaa gcggttgttc gatcaacttg
    19921 tctagtgcaa tcgaaacaac actgacctgc atttctactc caatataatt gatttcagga
    19981 tgggcttttg ccattccagt gatgaactgg cctttcccca taccaatctc aatgtggatt
    20041 ggatgatcgt tttcaaacaa ttcattccat tttcccttcc acaatgttgg atcactaatt
    20101 acaaaattcg ggtgagccgc taacatttcc gctgctcccg gacgatttcg gacacgcatt
    20161 atttatctcc attcctttcc tctaattaaa aaggtctggc caaatctccg cagagtttgg
    20221 gccaaacctc agccactgaa aattcgtttt aattgtaaaa tttcttcgtt catccgtcga
    20281 tgatcttcac gtttgcaata tctggtgatt tcttggagca aattcatggc cgcataccaa
    20341 tgaaattttt caagcatttc gtcagtcggg cgtatgccat aactgatcag ccactgattc
    20401 cagctagaca gtggcacata atttcctaaa atcgttgcaa tatccaccgc agggtctgca
    20461 aacatgattg aatcccaatc gactagatat aaataatttt tacatactaa ccaattacga
    20521 tggtttacat ctccgtgtac tactgataag ttttccttat ctattgacgg gatctgctca
    20581 cgaagaaaac gatggacaat ttgcagaaat tgatttttct ggagttcttc cggtaattct
    20641 ttttcatatt tttgcagcat cttctccggc gtcatgacac gaccaccgat acgggtcagc
    20701 atggttttaa gagaatttga atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt
    20761 tgaccaatct cattaggagt cagcaagcga ccttccagcc actcttgtgc tgtcaaagta
    20821 tcgccattcc ccgttcgttt tgtccaaaca agttttggtg taattccttc ccttgaaaga
    20881 gcagccaaca tcggagtcgt gtttcgttta ataaagactt tgtcctcatc acgcatgcct
    20941 atgtatgttt ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg gtctaactga
    21001 aactccatca tgcattctcc cgtcctctta ctctttgttt tgcagtactt attattctag
    21061 tcgcctgaac actacacgtc aagcacatat acgttctttt tcatttaatt gccattcaaa
    21121 aaaccagctt ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa cgctctattt
    21181 ttcattctca tttttaccct acacagcttt gttttcagtc tgacatcttc ttcaaacgag
    21241 ttggtacata tagtttaaca aagagcatag taacaatcaa ataagccgct agcggaaccc
    21301 atcgctgctg cccatctaat acgactgcag caatcaaacc aaaaataacc gacgcgataa
    21361 ttaataccca tgtgagtaga tactgaatcg cctgcttctt ctgattctta tggattggat
    21421 aaagctgaac gagaaccata tagcggaact ggttcgctaa aggtaacagt tggaaaccga
    21481 tcaaatataa aaataagaaa ccgataatca gactaaaata aaagtcgact actccggcaa
    21541 tcaataaccc acctataatt actaatcgga aataaagacc actaaaatca gaacctcttg
    21601 ccaatcttct tgcaaacaag taaagataag tgttttttga ttctctttta atatgagtca
    21661 atataggatc caaatagcgt cttcttttga tctttgccgt aatttcagga acatctgtga
    21721 ataaattgat aaattgatag atctgatgca acctatgttc ttctacatgc accattttct
    21781 cccaatccaa tcctttttct tgttgcttcc acaaaaacca gtaatataga cttgcctgta
    21841 ctaatgcacc tgctattcct gcccatatta gccaataaaa tgatagaaac aaaaagatca
    21901 gagaagaaaa aaaccagcct aatttccaat aacgaataac ttgttgatct agtcgaaaca
    21961 attctgctct cttgatttgc agatgactga atttcaaact ccataatatg atcagataga
    22021 cgaaaaacat cgaaaaggat tgctccgtac taaccaccac taatggcatg gccaatccgc
    22081 atgccaatat caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat
    22141 aggtttgcat ttctttttct ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag
    22201 tagccatctt acccacgaat aaacagagca tccaaaatac agagattatg agccctccaa
    22261 ttgcaaacgg tggctgcagg gttttcaacc aattagaata atataatccc acaccgccaa
    22321 tcaagaaaag acaaactaaa acaaaatgat cgttcaatac ataacgcata tatttcatca
    22381 ttttcttttg atgtcttgct aaacgttttt gaaaaaaact actcatgtgc ctgctccttt
    22441 gttaaagcaa tatagatttc gtccaaagaa gaatccggca gctgaaattc ttttctcaac
    22501 atctcaagag aaccgtctgc tcgtatctct ccgttatgta agaccacaaa acgatcacaa
    22561 tatttttctg cagtagctaa aatatgcgta gacatcaaaa taccggctcc ttcttttttc
    22621 atttctacca tcaattcaag caatgcattg atagctagtg gatccaagcc taaaaatggt
    22681 tcgtcgatga tatataaaga aggctcgatc aaaaaggcgc ataagatcat cactttctgt
    22741 ttcattccct ttgaaaaatt agctgggaac cagtcaagac gattagataa ccgaaaagtc
    22801 tccaacaact tttctgctct tttcattgct atatccatag gtatatcgta ggccatcgca
    22861 gtgacttcga tatgttctct caatgtcaat tcttcatata aagaaggagt ctctggaata
    22921 tacccaattt gtttgcgata ttcttccgta tttttcttta aggatagatg attgatttct
    22981 atcgttcctt ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt acttttccct
    23041 gctccgttca gtccaatcaa ccctactatc tctccaggct gtacatcgaa actgatatct
    23101 tttaacacag ggagatgtcc gtacccccct gtgacatgat cgataattaa actcattctt
    23161 tttcctccgc catttatttt tatttattat accacgaaga aaatgacggt attttgtttt
    23221 tttccttaca atagctttaa cttatggtaa agttaagctg aagtatttat tctttataga
    23281 aaggatgttt tatatggaaa actgtatttt ttgcaaaatc atcaatcaag aaatcccaag
    23341 ctataaaata tatgaagacg acaaggtcta tgctttttta gacatctctc aagcaacgaa
    23401 aggacacaca ttagttgtgc ctaaacgtca tgtagcagat atttttgaat atgatcccga
    23461 acttgcagga gaggtttttt cgcgtgtgcc taagattgct caagcattgg aaaaagcttt
    23521 tccagaaatg aaaggtctta atatcttgaa taacaaccgt gaactagcct atcaatctgt
    23581 ttttcattca catatccatt tagtccctcg ctacagcaaa gaggatgatt tttctattca
    23641 ctttgtcaat catcaagata gttatggatc ggaagaacta aaagctattc aagaaacaat
    23701 cgtaaagcag gtgtcctgcg atgattaaaa actttttcaa aggactgtta tttggagctg
    23761 ctgctggcac tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa acaagaaaga
    23821 aactgatcga agaattggaa gaagcaacaa ctctgacaaa tgatttaaat aacagcctga
    23881 atcattttaa aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg attcctgctt
    23941 tccaagaagc aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa ccacgtatca
    24001 cacaaatcaa tgaacaagta gaaaaactaa aagctgatct gccagagtat cctgaacaaa
    24061 tataataatg
//
LOCUS       DAHEYX010000017        24009 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHEYX010000017 DAHEYX010000000
VERSION     DAHEYX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.017"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="MB219_000476"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="MB219_000476"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCC8426212.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="MB219_000477"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="MB219_000477"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCC8426213.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="MB219_000478"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="MB219_000478"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCC8426214.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="MB219_000479"
     CDS             complement(3376..4683)
                     /locus_tag="MB219_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCC8426215.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="MB219_000480"
     CDS             complement(4686..5267)
                     /locus_tag="MB219_000480"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCC8426216.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="MB219_000481"
     CDS             complement(5839..7131)
                     /locus_tag="MB219_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8426217.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="MB219_000482"
     CDS             complement(7128..7751)
                     /locus_tag="MB219_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426218.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="MB219_000483"
     CDS             complement(7754..8590)
                     /locus_tag="MB219_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8426219.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="MB219_000484"
     CDS             complement(8607..9494)
                     /locus_tag="MB219_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8426220.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="MB219_000485"
     CDS             complement(9510..10793)
                     /locus_tag="MB219_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8426221.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="MB219_000486"
     CDS             complement(11476..12159)
                     /locus_tag="MB219_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCC8426222.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="MB219_000487"
     CDS             complement(12177..12794)
                     /locus_tag="MB219_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCC8426223.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="MB219_000488"
     CDS             complement(12804..13628)
                     /locus_tag="MB219_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCC8426224.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="MB219_000489"
     CDS             complement(13621..14181)
                     /locus_tag="MB219_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426225.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14354..15310
                     /locus_tag="MB219_000490"
     CDS             14354..15310
                     /locus_tag="MB219_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8426226.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15615..16571)
                     /locus_tag="MB219_000491"
     CDS             complement(15615..16571)
                     /locus_tag="MB219_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCC8426227.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16675..17277
                     /locus_tag="MB219_000492"
     CDS             16675..17277
                     /locus_tag="MB219_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCC8426228.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17365..18864)
                     /locus_tag="MB219_000493"
     CDS             complement(17365..18864)
                     /locus_tag="MB219_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCC8426229.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18880..19404)
                     /locus_tag="MB219_000494"
     CDS             complement(18880..19404)
                     /locus_tag="MB219_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCC8426230.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19702..20451
                     /locus_tag="MB219_000495"
     CDS             19702..20451
                     /locus_tag="MB219_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCC8426231.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20442..21227
                     /locus_tag="MB219_000496"
     CDS             20442..21227
                     /locus_tag="MB219_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCC8426232.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21196..21912
                     /locus_tag="MB219_000497"
     CDS             21196..21912
                     /locus_tag="MB219_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCC8426233.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22014..23048)
                     /locus_tag="MB219_000498"
     CDS             complement(22014..23048)
                     /locus_tag="MB219_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCC8426234.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23052..23975)
                     /locus_tag="MB219_000499"
     CDS             complement(23052..23975)
                     /locus_tag="MB219_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCC8426235.1"
                     /translation="MLSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYII
                     LILVSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRI
                     FLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEIS
                     QVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVK
                     VVFVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLAS
                     NASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7092 a         5186 c         4200 g         7531 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatcc tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttaa tgtttcaagt cctctcctct
    14281 ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa
    14341 ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca
    14401 tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac
    14461 ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta
    14521 tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca
    14581 gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc
    14641 catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt
    14701 gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat
    14761 aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa
    14821 agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga
    14881 tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt
    14941 tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg
    15001 taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt
    15061 cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg
    15121 tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga
    15181 agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg
    15241 ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa
    15301 aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
    15361 aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct
    15421 tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag
    15481 caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
    15541 acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc
    15601 tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata
    15661 aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga
    15721 ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt
    15781 tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata
    15841 tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc
    15901 acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc
    15961 cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa
    16021 tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt
    16081 ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta
    16141 cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc
    16201 gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa
    16261 accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg
    16321 aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct
    16381 gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc
    16441 ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat
    16501 aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt
    16561 cctatacgca tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag
    16621 ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa
    16681 tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt
    16741 atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt
    16801 ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc
    16861 aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat
    16921 gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca
    16981 gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg
    17041 ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt
    17101 atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag
    17161 aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca
    17221 aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt
    17281 tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt
    17341 gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt
    17401 tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat
    17461 ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt
    17521 tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc
    17581 gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg
    17641 tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc
    17701 gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc
    17761 ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg
    17821 ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc
    17881 gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt
    17941 gatttctttt tcttccagtt cgatttgttt ttggcgttct acgattttaa tctgcatttc
    18001 ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt
    18061 tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa
    18121 tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg
    18181 agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt
    18241 gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc
    18301 tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc
    18361 ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc
    18421 aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct
    18481 tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt
    18541 gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt
    18601 ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa
    18661 gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac
    18721 atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg
    18781 gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc
    18841 gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc
    18901 ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg
    18961 ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac
    19021 acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac
    19081 ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat
    19141 caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa
    19201 taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa
    19261 agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc
    19321 aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt
    19381 ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc
    19441 aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg
    19501 ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa
    19561 attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac
    19621 acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg
    19681 atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt
    19741 gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca
    19801 catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt
    19861 gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct
    19921 ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa
    19981 gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt
    20041 attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga
    20101 caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt
    20161 tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa
    20221 cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct
    20281 tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag
    20341 cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta
    20401 aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag
    20461 aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa
    20521 ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat
    20581 atataaactc catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg
    20641 gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa
    20701 attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt
    20761 ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg
    20821 attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag
    20881 ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg
    20941 tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg
    21001 acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga
    21061 agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg
    21121 gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa
    21181 aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca
    21241 gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga
    21301 aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat
    21361 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
    21421 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
    21481 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
    21541 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
    21601 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
    21661 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
    21721 gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc
    21781 aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg
    21841 gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca
    21901 gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca
    21961 actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt
    22021 aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca
    22081 cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct
    22141 acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat agcagggtct
    22201 ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca
    22261 tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct
    22321 tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat
    22381 ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata
    22441 tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct
    22501 gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc
    22561 gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta
    22621 aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta
    22681 tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac
    22741 gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg
    22801 actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg
    22861 gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg
    22921 atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat
    22981 ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct catactttct
    23041 tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat
    23101 acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt
    23161 gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc
    23221 atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa
    23281 actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac
    23341 catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt
    23401 gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac
    23461 ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta
    23521 actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg
    23581 ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt caagccactc
    23641 atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa
    23701 tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta
    23761 gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
    23821 aaaggttttg aaactagaat aagaataata taaataacta atccccaaat caagacattg
    23881 attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct
    23941 ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc
    24001 ccctttttt
//
LOCUS       DAHEYX010000018        23026 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHEYX010000018 DAHEYX010000000
VERSION     DAHEYX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.018"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="MB219_000500"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="MB219_000500"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCC8426236.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="MB219_000501"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="MB219_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCC8426237.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="MB219_000502"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="MB219_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCC8426238.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="MB219_000503"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="MB219_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCC8426239.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="MB219_000504"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="MB219_000504"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCC8426240.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="MB219_000505"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="MB219_000505"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCC8426241.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="MB219_000506"
     CDS             complement(8052..8522)
                     /locus_tag="MB219_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCC8426242.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="MB219_000507"
     CDS             complement(8568..8993)
                     /locus_tag="MB219_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCC8426243.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="MB219_000508"
     CDS             complement(9060..9329)
                     /locus_tag="MB219_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCC8426244.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="MB219_000509"
     CDS             complement(9340..10935)
                     /locus_tag="MB219_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCC8426245.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="MB219_000510"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="MB219_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCC8426246.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="MB219_000511"
     CDS             complement(14488..15048)
                     /locus_tag="MB219_000511"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCC8426247.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="MB219_000512"
     CDS             15401..16375
                     /locus_tag="MB219_000512"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCC8426248.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="MB219_000513"
     CDS             16545..17945
                     /locus_tag="MB219_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCC8426249.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="MB219_000514"
     CDS             complement(18023..18745)
                     /locus_tag="MB219_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8426250.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="MB219_000515"
     CDS             complement(18824..19330)
                     /locus_tag="MB219_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCC8426251.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="MB219_000516"
     CDS             19453..21252
                     /locus_tag="MB219_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCC8426252.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22858)
                     /locus_tag="MB219_000517"
     CDS             complement(21320..22858)
                     /locus_tag="MB219_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCC8426253.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT         6306 a         5002 c         4205 g         7513 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt
    21961 ctgttgctga agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg
    22021 atgattcttc tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag
    22081 cctctgcttg ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt
    22141 gttctgctag acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt
    22201 gacggttcaa gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt
    22261 tcagtacgtt caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag
    22321 cttggttttc agctaattct tttagtttag catcgttttc agcttttttg tcttctacag
    22381 cttttttatc ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca
    22441 ttgcttgaac gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt
    22501 agtttgagct tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac
    22561 gtttttcaat acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac
    22621 gcaatgtatc ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc
    22681 tttctaaggc atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct
    22741 tttgatcttg ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg
    22801 ctacggcagt caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc
    22861 ttcattcctc cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac
    22921 gatataaaaa ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt
    22981 ttcaaaacaa cttttaaaaa tgacaaaact gttcgtttca gaaaat
//
LOCUS       DAHEYX010000019        22822 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHEYX010000019 DAHEYX010000000
VERSION     DAHEYX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.019"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..368
                     /locus_tag="MB219_000518"
     CDS             <1..368
                     /locus_tag="MB219_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCC8426254.1"
                     /translation="FTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVGV
                     EDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLGE
                     EYVFNFADPDGNYFAVTGK"
     gene            548..1489
                     /locus_tag="MB219_000519"
     CDS             548..1489
                     /locus_tag="MB219_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCC8426255.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="MB219_000520"
     CDS             1558..2289
                     /locus_tag="MB219_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCC8426256.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="MB219_000521"
     CDS             2433..4613
                     /locus_tag="MB219_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCC8426257.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="MB219_000522"
     CDS             4633..5115
                     /locus_tag="MB219_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCC8426258.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="MB219_000523"
     CDS             5131..5892
                     /locus_tag="MB219_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCC8426259.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="MB219_000524"
     CDS             6023..7357
                     /locus_tag="MB219_000524"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCC8426260.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7664..8149
                     /locus_tag="MB219_000525"
     CDS             7664..8149
                     /locus_tag="MB219_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCC8426261.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8142..8825
                     /locus_tag="MB219_000526"
     CDS             8142..8825
                     /locus_tag="MB219_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCC8426262.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8951..11596
                     /gene="polA"
                     /locus_tag="MB219_000527"
     CDS             8951..11596
                     /gene="polA"
                     /locus_tag="MB219_000527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCC8426263.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11641..12477
                     /gene="mutM"
                     /locus_tag="MB219_000528"
     CDS             11641..12477
                     /gene="mutM"
                     /locus_tag="MB219_000528"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCC8426264.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12441..13070
                     /locus_tag="MB219_000529"
     CDS             12441..13070
                     /locus_tag="MB219_000529"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCC8426265.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13122..13454
                     /locus_tag="MB219_000530"
     CDS             13122..13454
                     /locus_tag="MB219_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCC8426266.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13766..14257
                     /gene="nrdR"
                     /locus_tag="MB219_000531"
     CDS             13766..14257
                     /gene="nrdR"
                     /locus_tag="MB219_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCC8426267.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14280..15653
                     /locus_tag="MB219_000532"
     CDS             14280..15653
                     /locus_tag="MB219_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCC8426268.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15653..16579
                     /gene="dnaI"
                     /locus_tag="MB219_000533"
     CDS             15653..16579
                     /gene="dnaI"
                     /locus_tag="MB219_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCC8426269.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16708..17148
                     /locus_tag="MB219_000534"
     CDS             16708..17148
                     /locus_tag="MB219_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCC8426270.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17292..17993
                     /locus_tag="MB219_000535"
     CDS             17292..17993
                     /locus_tag="MB219_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCC8426271.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17990..19111
                     /locus_tag="MB219_000536"
     CDS             17990..19111
                     /locus_tag="MB219_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCC8426272.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19126..20358
                     /gene="pepT"
                     /locus_tag="MB219_000537"
     CDS             19126..20358
                     /gene="pepT"
                     /locus_tag="MB219_000537"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCC8426273.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20490..21398
                     /locus_tag="MB219_000538"
     CDS             20490..21398
                     /locus_tag="MB219_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCC8426274.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21433..21705
                     /locus_tag="MB219_000539"
     CDS             21433..21705
                     /locus_tag="MB219_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCC8426275.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21716..22762
                     /locus_tag="MB219_000540"
     CDS             21716..22762
                     /locus_tag="MB219_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCC8426276.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7695 a         3981 c         5011 g         6135 t
ORIGIN      
        1 tgtttacgaa tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt
       61 tttggagagc agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tctcgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt tttcttggct gttcttatta tttgatgcag gacagctttt tcacgacctc
     7561 tgtaaactat aggaatctgt taaatcaagg gagcagaact tgccttttac gctacttttg
     7621 gtacaataat gtatgattaa aaataaactg gagggtttct atcttgaaca taggtaagcc
     7681 gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat ccttaacaga
     7741 atctattgaa gataaccagc ttttgctgta tttgggattg acgaatgatg caaatccttt
     7801 gtatatccaa catgactttg cacaaaaaac agaatatcaa cagccaatcg ttccatcagt
     7861 gatgctgatg gggatcatca caagtgcaat ttcaaagcat cttcctggac caggatcaca
     7921 tgttgtcaat ttttctgtca attttattga accggttttc cattatgaaa cactgacttt
     7981 tcagtttgag gtcataaaag tagataagat gaaggatgtg gtgacgattt ctgtagaagc
     8041 ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac cgccacaagt
     8101 tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg aatgaataat ccaagtacat
     8161 caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg atttttaggt atgggacttg
     8221 cccttagtgc agtcacttct tacttggctc tttgggtatt tcctttgcaa ttagcaactt
     8281 ttatcaataa ttttccacta ggctttgtgg gtctgtggat cgttgaactg attttagtaa
     8341 tggttctagg cgtaaaagca caaaagaatc cgagcttagc aatcggtgga tttattgcct
     8401 actctctttt gaatggcata acattagcgg ttactcttgc atactacgat attggaacag
     8461 taacgcaagc tttcttgaca gctactttga tgttcatggg gatgtctgtt gtcggagtaa
     8521 caacaaaaag agatcttagc ggggttggaa gagcaggaca ggcagcttta atcggagtga
     8581 tcattgcact agtcttgaac atcttcattt tgagaagtag cgtagttgag ctgttcatct
     8641 ccctactgtt agtcgtcatc tttgcaggga ttacagctta tgataaccaa agcatccgta
     8701 atatctatgt ccagacaaac ggaacagcac atagtggaat tgctgtattc atggcattga
     8761 agctgtattt ggactttatc aacttgttct tggcgtttct gagaatcttt ggacggaaca
     8821 actaaaaaag aaaaaaattc atcagttggg gctataccga attatttggt atggtcccgc
     8881 tttttctttt cgcattccgc aggattcttt gttagaatga ataagaactt tacagtaagg
     8941 agttaagaag atgacaaaaa ataaattatt actcgttgat gggaacagtg tggcttttcg
     9001 cgcattcttt gcgcttcaca attctcttga acgtttcaaa aatagaaacg gtcttcatac
     9061 aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag agcaaccgac
     9121 tcatgttctg gttgcgttcg atgcaggaaa aacgactttt cggacagaaa tgtatgcgga
     9181 gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc catatatacg
     9241 tgatttgttg acaggactag gggtacagta ttacgaattg cccaattatg aagcagatga
     9301 tattatcggt actttggcag aaaaagtaga caaagatcaa tttgatgtcg tcgtgctttc
     9361 aggtgatcgg gatttgactc aattagctag taaagaagta aaagtggata tcactgtaaa
     9421 aggtgtcagc gatatcgaaa gctatacacc agaacatgta gcggaaaaat atgatgggtt
     9481 gacgccaaaa cagattattg acatgaaggg actagccgga gatgcctctg acaatatccc
     9541 cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat atggctcagt
     9601 cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag aaaatctgat
     9661 aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg accatcaata cttcagcgcc
     9721 tgtggatgtc tctatcgact cattgaagta tgaagggaaa aatctcgata aactcgttcc
     9781 gttttataaa gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg tagaagaaga
     9841 agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg aaatgtttac
     9901 tacggatatg gcgttgtacg tggaaatgat gggcgacaat tatcatacag aggaaatcgt
     9961 cggagtagcg tgggggacag aaaagaaaat ttatgtcacg aacgatctat ctgtattcga
    10021 aaaccgagcg ttccattctt ggatcactga tccgactcgg ctgaaaaaag tatatgatgc
    10081 gaaacgaact tatgttgctt taaaccgcta tactggaaaa acgaaaggaa tcgattttga
    10141 tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa agcacagata tcgaaggaat
    10201 agctgctcat tacggataca acgatatcca atcagatgaa gctgtatacg gcaaaggggc
    10261 gaaaaaaggt ttaccagaag aagaggaact cttttttgct catttagcgc gaaaagtagc
    10321 tgcaatcaat gcgttgacac caaaattgag ccaagaactg gcagaaaaaa accaagaaga
    10381 tcttttccgg aaaatggaac tgccattgtc tatcattttg gcagagatgg aaataagcgg
    10441 gatcaaagta gatgcgactc gcttacaaga aatgaagggc gagttctctg ctcgcttaag
    10501 agagatcgaa caaaaaatct acgaagaagc cggcgaagaa ttcaatttga attcaccaaa
    10561 gcaattagga gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa aaacaaaaac
    10621 tggatattcc acagccgtag atgtactgga acagcttcgt gaacaagcac caatcgtaga
    10681 ggatatcctc acctatcgtc aaattgcaaa aattcaatct acctatgtcg agggactgct
    10741 gaaagtgatc caatcagatg gaaaagtgca tacccgttat gtccagacat tgacccaaac
    10801 aggacgatta agctctgttg atcctaattt gcagaacatc ccgattcgat tagatgaagg
    10861 gagaaagata cgtcaggcat ttgttcctag agaaaaagac tggctgattt attcttctga
    10921 ctattcacaa atcgagttac gcgtacttgc gcatatctca aatgatgaac atttgaaagc
    10981 tgccttccta gaaggtcaag atatccatgc cagcacagcg atgcgtgtat ttggaatcga
    11041 aaaggcagag gatgtcacac caaatatgcg taggcaggca aaagcagtga attttggtat
    11101 cgtgtatggc atctctgatt acggactttc acaaaatcta ggaatcagcc gaaaagcagc
    11161 acagcaatac atcgatacgt actttgaaaa atacccaggt gtcaaagaat atatggaaag
    11221 aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc gccgtcgata
    11281 tctgccagac atcaattcaa gaaattacaa tatccgttct tttgcggaac gaacagctat
    11341 caatacacca atccaaggaa gtgctgctga tattttgaaa atcgcgatga tcgaattgga
    11401 taaacgatta aaagaaacag gtcttcaagc tacgatgctg ttacaagttc atgatgaact
    11461 tgtcttcgag gtaccggaaa aagaacttga atcattagat aaattggtga aggaagttat
    11521 ggaacaagct gtttctcttc atgtaccact gatcactgac agcagttggg gaaaaacgtg
    11581 gtacgaagca aaataaacat tgtttaagac agagcaatat aatgaaggtg aaaagaaaaa
    11641 atgccagaat taccagaagt agaaaccgtt cgtaaaggat tggaacggtt agtagtagga
    11701 aaaacgattc aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc agagacaccc
    11761 atctttgagg cttctcttgt tggcgaaacc atccaatcca ttggacgtag agggaagttt
    11821 ttaatttttc atctcgatca ttgtgaactg atctcacact tgcgaatgga aggaaaatat
    11881 caatttacga aagaaaatac accaatcgat aaacatacgc atgtactttt cttttttgag
    11941 gatggaagcc aacttcgcta caacgatgta cgaaaatttg gtcgtatgac aatcgtcgaa
    12001 aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg gacctgagcc tttgccagat
    12061 agcttcttat tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc aatcaagcca
    12121 ttgctgctag accaacgtct agtgactggt ctaggtaata tttatgtaga tgaagcatta
    12181 tgggaagcga agatccatcc agaacaaccg gcgaacacac tgcgccccaa agaggtagaa
    12241 caattacgtc tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg aggtaccacg
    12301 attcgcagtt atttgaatgc attaggagaa tcaggcggat ttcaagtagc actgcatgtc
    12361 tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa aacaaaagtg
    12421 gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc aaagtaggat
    12481 ttgttttagg tttgacgggt ggaatagcta ccggtaaaag tacggcagcc aaagtctttc
    12541 agtctcatgg atttcctttg atcgatggcg atgtgattgc cagagaaact gttgagcctg
    12601 gaacgcttgc tctgaggaaa attgtatcag tgtttggcca agaggtattg cagcctgatg
    12661 ggcaactaga tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa aaacggcaaa
    12721 aacttgatca gctattagat ccgtttatcc gaaaagcgat aaaagatcaa atcgcctctc
    12781 taagttccaa tcatccttta gtaattgtag atatcccatt gctctacgaa ggtcattatg
    12841 accactatat ggatgcagta gctgtcgttt atactacgcc agaaactcag cttcagcgtt
    12901 tgatgaaacg taatcaactg acgctagaac aagcgcagca gagaattagt agtcaacttc
    12961 caatcgaaga aaaaaagcag cgtgcggata ttttgttcga caacaatgga acaaaagaaa
    13021 aacttgtggg acaaatcgaa aattggcttt ttgctaatcg ctttctttga gtatctgtgt
    13081 caattttgct atcctgagta tatagaaagt gagggatagc gatgggatca ttaaaaaatc
    13141 aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc aataaagaaa
    13201 atctaatcga tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa aagcagtacc
    13261 aagcaagaat cgatgaaata gaagaccagc aggcagaaga agaagcgaag cggcaagatt
    13321 tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc ccagaaagtg
    13381 accacattcc cgatgagcaa aaaatcgagg aacagcgaat gaaacactat acaggaatcg
    13441 aagagaagtt ataaaatagt aaaaaatcga gatgtgactt gaagcagatg agaaggtgtt
    13501 catctgcttc aagttttttg tgctagatga ctaaatcagc ttttgtgtaa gttatttctc
    13561 agtaatcaag gtgatcagta ttggaaaatt gaaatttagt aaggacaggg gacacaatta
    13621 cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt ttgggtagga cagtcgcgtt
    13681 tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga aagacacatc ctttatataa
    13741 aatgcgatgc aagaggtgag acaaaatgag atgtcccaga tgtcaacata ataattcacg
    13801 agtaatcgac agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc gcgaatgtga
    13861 gaattgttcg ttccgattca ctacatttga acgaattgaa gctgctccgt tattagtaat
    13921 caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa attttaagag gactcatccg
    13981 ttcagcagaa aaaagacctg tagctatgga acaaatggaa caaatcgtca accatgtcga
    14041 aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg aatctaattg gtgaatatgt
    14101 gatggaagat ttagttgatt tagacgaaat cgcttatatt cgttttgcaa gtgtttatcg
    14161 ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg ataaagcaaa
    14221 agaaaataat aaagagaata ataaagaaaa caattaagaa acaaaaagga ggcatttcct
    14281 tggaaaatgc ttggaaagag cttcaaccaa aaaatattta ccaaggaaga aaagcggctc
    14341 cgctgagaga agacagcaag aacgccttgc tgtatcttta tcagccacta atcggcggag
    14401 aagccttgag tatctatttc accctgttat cagaaatctc tcaagaatct ggcaaaggtc
    14461 ctgaagggat gcatgcagat ctgcttagta gtctaggctg cggtatcccg caattttatg
    14521 atgcaagaaa aaagctagaa ggaatcggct tactggatgt ttattttaaa gaagatagtg
    14581 atctgggacc tcgttttatt tatgagctgt tagaaccact cattcctaaa gcatttttca
    14641 aagactctgt tctggctttt cttcttctgg aaaaagttgg tgaacgacga ttccagaagc
    14701 ttacccaatt ctttgaacct aaaaggcata cagcagaggg atatcaaaaa atcacaaaaa
    14761 aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa gaaaacatcg
    14821 aatcgctgaa taaatctttt gaagaatctg aatcagtagc attacttgaa gcagattcga
    14881 cattcgattg gcagtttttg aatgattggc tggcaaaaaa acatattgca agtcttgatg
    14941 atccaatcat caaacaagtc gacatgtatc atcagcttta cggtttcgat gaattgacac
    15001 ttggcgaact gatcagtcaa tcttatgact ttagtgaaca aaaagtatct gaaaaagaac
    15061 tgcaaaaagc cattttggca atgcagagac gtccggtgaa gcaaacaaaa gaaacagttg
    15121 cggtttctga aaacagtaat gaaccctcgt caaacgaaca tttgacaaac gcagcgcttc
    15181 agctgataaa agaagcacag agcattccac cgatgaaata tctggaagca atcaagtcag
    15241 aaaaaggcgg atatgtttct aaacaagaaa actggttact gcaagatctt gtttctcaat
    15301 caggtctttc cagtagcgtg atcaatgttt tgctgaatta tgtactagtg atcaagaata
    15361 atgctagctt aaatgccagt tatgtaaata cagtggcaaa tgaatgggca caaaagaaaa
    15421 tcgccacagc agaagaagcg atccagcaca ttcggcaaat aagtaatcaa gcaaaacaat
    15481 caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga cgggaaaagc
    15541 ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag aaaaaagcag
    15601 agattgaccg ccgttttaaa gaatacctgg caaaaaagga gggtgatatc tagtggaaga
    15661 cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat tatcaagaaa agtatgctga
    15721 aatgatcgct gaagtcttaa atgatgcgga tgttcaagct tttctaagcg cacaccaaga
    15781 agaattgaca caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat tcgtgcaaga
    15841 aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct cccggctatg agccgaagct
    15901 gacgttgaat tttcattatg tggatgtcac ttatgtacca acggaagcat tgttagcacg
    15961 gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg gatatgccaa aagatattca
    16021 atcggcttct tttgaaaact ttgaacgtac agatggacga ggatatgcga gcttagaagc
    16081 cctagatttt gtagagaaat atactgctga tccaaaaggg tatcataaag gactgtatct
    16141 agtaggaagt tttggtattg gaaaaacata tttattagga gcaacagcga gagaattagc
    16201 gattgctgga ttcaccacga cgctagtcca ttttccgaca tttgccgttg agatgaaaca
    16261 ggcaatcgga aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt cacctatttt
    16321 gatgatcgat gacattggtg cagatgcgat gagtagttgg atacgagacg aagtattcgg
    16381 tgttatcctt caatatcgga tgcaggaaca gttaccaacc ttcttctcat cgaattttac
    16441 aatggatgaa ttggaacagc atttgtccgt cactcaacgt ggagatgaag agccgctaaa
    16501 agcgaaacgg ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa tgactggtcg
    16561 caaccgacgc aatggttgaa cagttgacaa gtccaactga catttgacct agtagtaaaa
    16621 gcaggtcagc gaatcattat atggacagaa atgaccggag acaatcaccg caacaagtaa
    16681 tgcagaaact taaacggagg attttttatg agggctattt tttctagtat atctgtagtt
    16741 ggtctatttt ttcttttagc aggctgcggt agcgcagata gcaacacagg ttctgttgat
    16801 tcgattgatg cgggtcaaac agtaaaaaca agcagtacga aaacggagag acaaagcata
    16861 tttgtcggaa cgattgaaag tatcactcaa gtagaagcag aagaagaaac gcttcagttg
    16921 aaactcgtcg atgtcatggc caaagaagat ccagcgaata tgggaacgtc attcagcaat
    16981 gacggagtga tcttaaatgc tactgctgaa aaaataagcg tggacataaa tgaattgaaa
    17041 atcggtgaca aagtccaatt tacattagtt gagatgccga tcatgacgat gtctatccca
    17101 ccgcaagttc ctggagattc gattattgat gtttcattga acaaataacc aataacattt
    17161 tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat
    17221 gggaattttt ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa
    17281 ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg
    17341 tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat
    17401 tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg
    17461 aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg
    17521 caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta
    17581 tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg
    17641 gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc
    17701 aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg
    17761 aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt
    17821 tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc
    17881 ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc
    17941 aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag
    18001 cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg
    18061 agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac
    18121 attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat
    18181 tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga
    18241 aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat
    18301 ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga
    18361 gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt
    18421 ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga
    18481 ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc
    18541 acaaccggcg attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt
    18601 tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta
    18661 tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg
    18721 acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga
    18781 agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa
    18841 gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag
    18901 agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc
    18961 tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat
    19021 cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga
    19081 aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt
    19141 ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg
    19201 actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag
    19261 ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca
    19321 agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat
    19381 tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc
    19441 gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat
    19501 tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa
    19561 tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct
    19621 cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa
    19681 ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt
    19741 gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat
    19801 gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt
    19861 cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc
    19921 catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat
    19981 catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg
    20041 aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg
    20101 aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag
    20161 cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct
    20221 tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc
    20281 gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa
    20341 ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg
    20401 atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc
    20461 ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa
    20521 gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa
    20581 agcagccaga attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat
    20641 tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt ggagacggca
    20701 cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac
    20761 ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag
    20821 aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg
    20881 atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca
    20941 gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat
    21001 tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa
    21061 aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg
    21121 actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt
    21181 tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca
    21241 ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc
    21301 gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat
    21361 taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt
    21421 ggaagaagag taatggatgt attgttacac aatcctttgt ggttttacct tttcattgtg
    21481 aatttttact tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg
    21541 cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg
    21601 atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct
    21661 gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc
    21721 gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc
    21781 cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac
    21841 cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg
    21901 gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt
    21961 cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat
    22021 ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac
    22081 agcactccat atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt
    22141 catccaattc tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg
    22201 tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt
    22261 tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg
    22321 ggtggataca tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca
    22381 agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta
    22441 tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat
    22501 ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg
    22561 ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt
    22621 ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt
    22681 gtatgtgatt gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga
    22741 gaataaagaa aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga
    22801 aaaacgttat ggataagata at
//
LOCUS       DAHEYX010000020        22775 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHEYX010000020 DAHEYX010000000
VERSION     DAHEYX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.020"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            60..470
                     /locus_tag="MB219_000541"
     CDS             60..470
                     /locus_tag="MB219_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCC8426277.1"
                     /translation="MNFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            463..1164
                     /locus_tag="MB219_000542"
     CDS             463..1164
                     /locus_tag="MB219_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCC8426278.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1164..2750
                     /locus_tag="MB219_000543"
     CDS             1164..2750
                     /locus_tag="MB219_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426279.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2769..2990
                     /locus_tag="MB219_000544"
     CDS             2769..2990
                     /locus_tag="MB219_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCC8426280.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2987..3748
                     /locus_tag="MB219_000545"
     CDS             2987..3748
                     /locus_tag="MB219_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCC8426281.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4128..4637
                     /locus_tag="MB219_000546"
     CDS             4128..4637
                     /locus_tag="MB219_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCC8426282.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4712..5251)
                     /locus_tag="MB219_000547"
     CDS             complement(4712..5251)
                     /locus_tag="MB219_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCC8426283.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5391..6002)
                     /gene="rpsD"
                     /locus_tag="MB219_000548"
     CDS             complement(5391..6002)
                     /gene="rpsD"
                     /locus_tag="MB219_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCC8426284.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6264..7115
                     /locus_tag="MB219_000549"
     CDS             6264..7115
                     /locus_tag="MB219_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCC8426285.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7143..7778
                     /locus_tag="MB219_000550"
     CDS             7143..7778
                     /locus_tag="MB219_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCC8426286.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7798..8571
                     /locus_tag="MB219_000551"
     CDS             7798..8571
                     /locus_tag="MB219_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8426287.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8979..9776
                     /locus_tag="MB219_000552"
     CDS             8979..9776
                     /locus_tag="MB219_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCC8426288.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9754..10167
                     /locus_tag="MB219_000553"
     CDS             9754..10167
                     /locus_tag="MB219_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCC8426289.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10151..12796
                     /gene="mutS"
                     /locus_tag="MB219_000554"
     CDS             10151..12796
                     /gene="mutS"
                     /locus_tag="MB219_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCC8426290.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12814..14922
                     /gene="mutL"
                     /locus_tag="MB219_000555"
     CDS             12814..14922
                     /gene="mutL"
                     /locus_tag="MB219_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCC8426291.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14944..15504
                     /gene="maf"
                     /locus_tag="MB219_000556"
     CDS             14944..15504
                     /gene="maf"
                     /locus_tag="MB219_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCC8426292.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15589..17577)
                     /gene="treP"
                     /locus_tag="MB219_000557"
     CDS             complement(15589..17577)
                     /gene="treP"
                     /locus_tag="MB219_000557"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCC8426293.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17800..18513
                     /gene="treR"
                     /locus_tag="MB219_000558"
     CDS             17800..18513
                     /gene="treR"
                     /locus_tag="MB219_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCC8426294.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18590..19036
                     /gene="msrB"
                     /locus_tag="MB219_000559"
     CDS             18590..19036
                     /gene="msrB"
                     /locus_tag="MB219_000559"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCC8426295.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19206..19808
                     /gene="ruvA"
                     /locus_tag="MB219_000560"
     CDS             19206..19808
                     /gene="ruvA"
                     /locus_tag="MB219_000560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCC8426296.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19821..20822
                     /gene="ruvB"
                     /locus_tag="MB219_000561"
     CDS             19821..20822
                     /gene="ruvB"
                     /locus_tag="MB219_000561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCC8426297.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20851..21429
                     /locus_tag="MB219_000562"
     CDS             20851..21429
                     /locus_tag="MB219_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8426298.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21481..21963
                     /locus_tag="MB219_000563"
     CDS             21481..21963
                     /locus_tag="MB219_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCC8426299.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22080..22454
                     /locus_tag="MB219_000564"
     CDS             22080..22454
                     /locus_tag="MB219_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426300.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7409 a         4071 c         4928 g         6367 t
ORIGIN      
        1 gaaactttca gaaaaggttt gttagtaaaa tgaaagctat acgtaaggag tgttgtttga
       61 tgaatttttt tcaagagttt ttcattatta ttttcttttc tgtattagga gaaggcatac
      121 ggatttttgc tcatttacct attcctggaa gtatcttagg tattcttttt ttatttttag
      181 catttgaaat gcatcttgtt gatcctgaaa aaatcggagc aactggtgat tttttattga
      241 ataatctagc cattttattt gttcctgctg gtgtaggttt attggaatat tttgatgata
      301 ttgcttcgat atggcctgtt ttactgggag cagtcgtcgt gtgttccatc gtcacgatgg
      361 ttgcagcggg aaaaacggca gaaggtgtag aagctttgct gggctatgtc cgtaagaaaa
      421 aagcagaaag ggtatctgta aacgaaatgg aggagaatca aattgactga gttttttgtg
      481 aatccattat ttggcttaag tttgacttta ggagtgtacg tattatctga caaatttttg
      541 cggaaatata aactgcctgt tttgaatcct ctcgtattat cgattgttgt tattatctta
      601 tttttgcatt tttctgggat cagctataaa gactataata tcgggggcaa ttttttgact
      661 atgctgatta ctcctgcgac agtggcactt gccatccctc tttataaaaa cttccgttta
      721 ctgaaagaga atttttatcc tgtaatggct gcgattttag caggaatagt cgcaaattgc
      781 ttggttagtg tagggttcgg ttacttcttc tctcttcata aagagatggt gatctcatta
      841 ctacctaaat ctgtaacgac ggctatttct gtcgatttat ctcatacgat gggcggggtg
      901 aatgccgtga cacttgctat tgttgtctct acaggtattt ttggttcgct gatcgccact
      961 catattttcc gcctattcaa gatcgaaagt ccagttgctc gcggtgtggc gttaggaagt
     1021 acttctcatg ctattggtac agcaaaagca atcgagattg gtgaaataga aggaatcatt
     1081 tccggacttg cgatatgcgt caacggaata ttgaccgttt tattgctacc gttattcttt
     1141 cagccttttg ctggcttgtt ttgatgcgaa aaccactatt ttatgctaca ataaccaaaa
     1201 cgttcgagca taaaaaagga gggaaagacg tgtatttgtc ttccgagtat cttaaacaat
     1261 ttgataaaga tctatattat aagattctcc tcctagaaag ttttgaggat caagcttggc
     1321 ataccgctgc acaattagcg caagttgttc agctggatgc ccgcagcgtg tcgaaatacc
     1381 tgaacgagct gtctaaaaac tatcagcagt ttagcggtaa gacacaccct ttgtttacaa
     1441 aaaatcaccg ttctggttat aacttttatg atacgctcga ttcaattgaa catgagcgtt
     1501 ttttgatcta tctcgtgcaa agtacgctga aattccagtt gctgcacgac attttttttg
     1561 aagaattcca tacaatgtac caatttgcgc aaaagcatta cattagtgaa tctactgctc
     1621 atcgaaaaat caatgaatgg aaacagcaac tgcaaactta cggaattcgg ctgcaacgag
     1681 gtacatatat cgctcaaggc gaagaagaaa tcattcgact ttacttgcat atgacattct
     1741 ggcagttgtt tcgagggaaa atctggccat ttgaaacgat ttctcaaatg gacgtaaaga
     1801 atatggcaga acatatcatg gcttttttta atgttcgtct gaacgagatc aaaaaaagac
     1861 gattggaata tatgctaggt gccttttttt taagaaaatc acagaagcat tatgttgtat
     1921 tgaatgagaa aaaaagacgg ctgatttctg acaatctgtt gttccagcga ttttgccagg
     1981 tcatggagcc ggtatttcct aattattttc aagtagaaga cgagttaggc gcattatttc
     2041 ttgtactgat gactcgtgaa gaatattata gtgatcctaa gatacggaag aaaatttttg
     2101 attttcatca acaagcaaaa acaccgccct ttacagccct ttcagaagca aaagccgctc
     2161 tttctcttta tcaggaagag caggggctgc cagcagaaaa tctaaccttc gaggcagaaa
     2221 actatttatt cagcagtcat tttttcgcct atctttttcc taatgcgaaa gaaacgattg
     2281 atggcaatag tagtgatttt attaatcatt tagttattga aaacaaggaa ttgaaacaat
     2341 ggctcgtaca tttttttgaa agtagacaca aacatcccaa tcatctggca tttaaaaatc
     2401 atgcgttttt gatggggcgg tatctgactg tgtttaaaac attaggagcc tttactccac
     2461 agcttccaaa gatcacgatc cttttaatga cagatttccc attatttgaa gagcaactgt
     2521 tagaggaggg attacggaca tttttccgta atgagtatca gctgattttt ctgccaacag
     2581 attatcgggg gagagaagtt gatcttctga tttcgacaag taaagtccac cgcaaaccat
     2641 gggcggatct tgactatttt attgtgactg aagaactaaa attaattgat tacatccaac
     2701 tatctcaaaa atttgaaatg attcaaaaac aaaaactatt aaaacaataa caaagaagga
     2761 gaaaaaagat gacatttgaa gaaattttac cagaattgaa aaaaggtgca aagattattc
     2821 gtaaaggatg gagtggattt gaactttatg ttgttctcgt atccgatgat gaatttgatg
     2881 gctgtccggt gacgccgtac ttattgatca aaacaagtga cgaaggtttt tccagcttcg
     2941 ctcctacagt ttgtgatatt ttagcagatg attgggcgat cgtagaatga aaaaccagta
     3001 tccagatcta gctaaacagg ttgtatttat cacaggagct gcttctggaa ttggtgctgc
     3061 gcaagcccga gcgtttcttg aacaagatgc ttttgtattt ggattagatc aaaagttcgg
     3121 taaaatggaa gaaatttctt tgcgatttcc tgaaactttt gcctacgcac taggtgatgt
     3181 tcgacaaatg aaagaactcc aacaagcggt atcgaaatgc caaaatcatt ttggtgaagt
     3241 tacgatttta ctgaatacag cggggatatt ggatgcctac aaacccttga tggaaactga
     3301 cgaatcgctg tgggatctga tttatgagac gaatgtgaaa agcatgtatc agctgacaaa
     3361 aatcatcctt cctgatatga tccgccaaaa aagcggaacg atcatcaata tggcttcgat
     3421 tgctggcttg attgctggtg gaggtgggat cgcttataca agcgcgaaac atgcgattgt
     3481 cggatttacc aaacaactcg ctctggatta cgcaggacag ggtatccgag taaaaggaat
     3541 tgctccagga gccatccaaa caccaatgaa tgcggctgat tttgaaggga aaggtgagat
     3601 ggcagagtgg gtggcaaatg aaacaccagt caagcgatgg gcacagccgg aagaagtagc
     3661 tgagctgacg ttatttcttg cctctcctca agcaagctac atacaaggaa cgatcatccc
     3721 aatcgacggc ggatggttgt taaaataacc ttgtgttttt ttcaagcttc tgtatgataa
     3781 aactaaagag agaaacaacg gttgatgata aaaaaagtcg atgactgttc ccgacattcg
     3841 atgccgaaca gccagttcaa aaaattcttt ttaataatcg ttaatattcg aaactaagcg
     3901 gcttttcacg acctcttgat ataaacgata ttcaaaagct tgcaactgcg gtaacaagtc
     3961 cagccaaatg aaaaatataa ggagctattt ttatttgacc attcttatta cttgttgcag
     4021 ggcagctttt tcactacctt ctgcgacact tcagttggaa ttttcttccc atcctttggt
     4081 cgcggtcatt accaagataa aattatcttg caaaggagaa atagaacatg gaaaaaaata
     4141 atacccgttc agccaaacgc tggacaacga cagatgtgac tcggatggcg ctagtcactg
     4201 gtttatatgt ggcagtgacg ctggttttgt ctgtctttag ctttggagtg attcagattc
     4261 gtctctcgga gatgttcaac tatctgtcct tgtataacaa aagatacatc gtcgcggtaa
     4321 cagcgggagt agctttggct aatattgctt ctcctcttgg actgatagat gttgcagtag
     4381 gaagcgtcag cacgctgctt gtattgctta tcaactataa aatcactagt cgcatcaaaa
     4441 acatgaaaat caaaatggcc gtgacagcat gcgtatttgc tttttccatg tttacagtag
     4501 ctggtcagct aacgatttta tatcaactac cttttttctt gaattggtgg gtgattgctt
     4561 taggagaatt ggcatcaatg attgtcggtg ggataattat ttattggatc ggtaaaaaag
     4621 tggatttgtc aaaataaaga tctgtttaat ttaaaatagg gcagcatgga agagactgaa
     4681 tgttgagtct tcttccatgc tgcttttttg attaatttgt atgaagaaat cgatttagcc
     4741 gttttttcat caagataccg atataagctg ctccagttgc cttgattgct tccggcagta
     4801 aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt aagatcacca cttattttca
     4861 gccagatcgt tccgcagatc aatgcagcaa gagagcccat tagattggca aagattgcat
     4921 ataagtaatg agcatccgtt ctttcgatca tccaaccagt tatccacgtt tgtaagagaa
     4981 atccaatcaa atatccgcca gttggcccaa ataaaacagc tatacctcca gtcatccctg
     5041 aaaatacagg aagaccaatc aagccaagta aaagataaac tccaacagat atcaatccta
     5101 gttttcttcc gagaaaagtt gctgttaatc caatagcaaa tgtttgccct gtcaaaggaa
     5161 ccaaaccaaa aggaatggtc atctgagaaa ggacagaaat aatcgcggca aattccgcag
     5221 caagtatcaa ttctttcaat gttcggttca taacgccctc ttttcgttaa cttaatattt
     5281 ttatttggtt aacgaaattg taaaacaaag tgattacttt gtaaatagaa aatataaaaa
     5341 aagaccagct atccaataga tagcggtctt tttcaagaaa gcttttgaaa ttataagctt
     5401 tggttgtagt attcaacgac aagagcttcg tcgatttctg gatatagttc gtcacgttct
     5461 ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa agcttacgaa tgctgggcgt
     5521 ccaacagttg cttctacagc ttcttttact gttacgatgt tttgtgattt ttcacgaact
     5581 gagatcactt gacccacttc tacgtggtat gaagggatat caacgcgttt gccatcaact
     5641 aaaatgtgac catggttaac taattgacgt gcttgacgac gagtcgttgc aagtcctaaa
     5701 cggtaaacaa cattgtctaa acgttgttct agtaagatca tgaagttaac accatgttta
     5761 ccttctttga ttttgctagc ttttacgaac aagttacgga attgacgttc gttcattccg
     5821 tacatgtgac gaagtttttg tttttcagtt aattgcatgc cgtattctga ttgtttgcca
     5881 cggctattag gtccatgttg acctggtgcg taagggcgac gtgctaattc ttttcctgtg
     5941 cctgacaatg agatgcctaa gcgacgagaa attttccatg atggtcctgt ataacgagac
     6001 attaaaattt cctccaatga atataagttt ttggagtaaa ataatccgtt gaaaaattca
     6061 taatcatata gtttgttctt caatcttcac ctttgcagcc gcggttacac aatcgaaccc
     6121 gagacggggc aacaaactag ggatgagtta ttatttttct gctgcattat tttacacaaa
     6181 ttacagtata tgatagatca tccacgaatg tcaagataat tagtacataa aaacgcttta
     6241 caaacctagg ctgtctttat aaaatgagag ggtatgtgca aatggaaaaa agcgaaggaa
     6301 gtttaaggag aaaatcaatg aatccggtat caccattatt tgagaaaatc gatgcttcaa
     6361 ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg ttacgctgtt cgagcggttc
     6421 tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt tgcgatcgcg atgaaggggg
     6481 aagatatcgc gcatggtcta ggaaagattt tatacgcatt tatgttcagc tggggtctgg
     6541 tcatgattct atacatgaac gccgaattag gaacatcgaa tatgctttat atgaccgtgg
     6601 gtgtttttcg gcagagactt gctttaagta aggcagccaa gattctattt gcctgtattt
     6661 ttttcaatat gattggcggt atattatgtg gatttctaat ttctatgact acgccatttc
     6721 aagatttaac gttggacaac tttatgttag aatcaatagc agtaaaacta gcaaaacctt
     6781 cagcaacgat tttggtggaa gggatgtttg cgaatatcgt tgtcaatacc gctgtactga
     6841 tcagcatgcg gatgaaagag gatgtcggaa aagtgatcac agtcgtattc atcattttca
     6901 tttttgcttt tttaggatat gaacacgtga tagcgaattt tcctgccttt acgcttactt
     6961 actttgcttc tcacggacag atggatggaa tgacagtcag caatgtgtta cataatttgt
     7021 tttttgcttt ggcaggaaat tatattggtg gcggtttagt aatgggactg ggatatgctt
     7081 ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt cgttcattaa aggggaagtg
     7141 ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc gttacaaatg gaaaagtaca
     7201 tgaaaaatct tttttccttt gctggagtac ctaagccgga acgcgctctt ttacaacaac
     7261 cgttactgga tgagagcaaa aatttatcta tttctgaagt tttagctttt gttcattatt
     7321 attatgaaca aatcgaaaga gagtatcagt atgtagcgat cgatttagtc cagaaaaaca
     7381 tcaaacggct tacatttgaa gacttagtcg tattgttgcc actagttcag aaaaaagaat
     7441 ggtgggacag catcgactct tggagaaaag tatacagcac atggtgtaaa gcctacccag
     7501 agcagcgtga agaagtcttt caattgtttg aaaaaaagga agatttttgg ctacgtcgaa
     7561 tagcaatcac gttgcaatta ggttataaac agcaaacaga cacaggacta ttgatccgag
     7621 caatagacga agatcgtcaa acaaaggaat tctttatcca aaaagcaatt ggttgggcat
     7681 tacgcgatta tagtaagacc gatgccgatt gggtaaaaag acagctgaca acagatttga
     7741 gtaagttagc tgttcgagag gcaagtaagt atttataaga aagcgagacg acaagagatg
     7801 agacgcaaat tatttgcttt tgatattgat ggaacgttgt tagattcaaa caaacaagct
     7861 ttagatagca caagagaagc attggaaaaa ttacgcaaag ccggtcatct ggtcacgatt
     7921 gctacagggc ggagccgatt tcatgcgcaa gagatcattc gcgatcttga ttttacgaac
     7981 tacattttat gtaatggagc ggccggtttt ttagaccatg aacaagtata taagaattta
     8041 ctagatcgcg accagttgga tgcgttcgtt caagaagcgc atcatgagca gatcgataca
     8101 gcttttgtca gcttagacaa tatcaagcgt tcttcttcca atgatgtaac ggtgatggaa
     8161 gaagcgatgc attctttcgg tgctgtcttg cctgaattag atcagttttt tgcagaggaa
     8221 caagaaatat accaagcgtt agcatttttc gatcaaacat atgatgggaa gttctcttca
     8281 tatgataaaa ttcgatttgt ccgctggcat gagaacagtg tagatgtcgt acctaaaaat
     8341 ggttctaaag cagcgacaat tctgcactta gcagaacaag taggcatccc tcatgaagac
     8401 atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt tgcgcatgtc aggaattggg
     8461 atagctatgg ggaatgctgt gccagaagta caagcagaag ctaagatggt cactgataca
     8521 aacgatcagg atggaatttg gaaggcattg aaagagttaa gcgtgattta aaagaggttg
     8581 tgaaaaagct gttctgcatc aagtaataag cccagccaaa caaaaatagc tcctcctatt
     8641 tttcgttttt ctgggcttat taccgcagat gcaagctttc gaacaccgtt taaggaagag
     8701 gtcgtgacag aagtggtcag tttcaagaaa taagaaggga tttacgaaaa ttgcttctca
     8761 aatttttgta aaattacggc ttatttctga agaagtagat gaaacaacgt ttattcggga
     8821 aatgggactg tgacaagatg tttgtcatag tcccattttt ttattttatt tttcaaaact
     8881 aagaaatacc aggaaagagt tccttttgtt tgaccttttc tttcttattg aaaacaacta
     8941 taatatagag tgatgttaaa agagaagagg aatttagtgt gcgggtattg tttattggtg
     9001 atgtagtagg ttcaatggga cgagagatga ttactgaata tcttcctaga ttgaagaaga
     9061 agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc cgcttctgga cgtggaatca
     9121 cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga tgtcgtgacg atggggaatc
     9181 atacgtggga caaccgcgga atctttgagt ttgtaaatga agctaaaaag atggttcgtc
     9241 cggcaaattt tccggaaggg acgcctggac aagggatggt ctttgtcaaa gtgaaccaag
     9301 tcgaactggc agtgatcaac atgcaggctc gttcttttat ggtggacttg gatgatcctt
     9361 ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg tacgccgatc atttttgtag
     9421 attttcatgg agaaacaaca agtgaaaaac aagcaatggg ctggttttta gatgggaaag
     9481 tttcagcagt ggtcggtaca catacacacg ttcagacaaa tgatgcgcga attttgccaa
     9541 gaggaacagc gtatctgaca gacgttggga tgacaggtcc ctatgatggt attttgggta
     9601 tgcgcagaga acctgtaatc gaaaagtttc tgactgcatt accaaaacgt tttgaagttg
     9661 tggaacaagg ccgatcgatt ttaagcggct gtatcttgga attggatgat acgacaggtc
     9721 atgcaaaaga aatccagctg attcagatca acgatgatcg accgttcatg gagtagaaag
     9781 agaggcagtt ttttgcagca ggagccggaa attcaaaaag aagtaaggaa actgacaaaa
     9841 cttttaagag agaatgaaac gatcattcgc tacaaagaac ttgaagaaaa gatccagcaa
     9901 aaccagtatt tagctgagtt gagagaaaaa atcaaacaag cgcaaaaaga tgctgttcat
     9961 tttgcccact atgacaaacc agcagcagaa aaagaagcaa tcaaacaagc ggatcaattc
    10021 atgcaagaat ttgatcagca tccgcttgtt gtagcttata gaaaacagct gttggaagca
    10081 gatgatctgc ttcatcattt aacaactatg atacaagaag aaatcaatgg acaaatagag
    10141 gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg atggaacaat atctaagcat
    10201 caaggcccaa taccaagatg cttttttgtt ctatcgttta ggagactttt atgaactatt
    10261 ttacgaagat gcgatcaaag cgtcacagct tttggaatta acgttgacga gcagaaatcg
    10321 aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac catgcagcac aaggatatat
    10381 cgatgccttg attgaaaaag gctataaagt agcgatttgc gagcaggtag aagatccaaa
    10441 gacgactaaa gggatggtca agcgcgaagt gatccagcta gtcactcctg gtacagtcat
    10501 ggacagcaaa ggattgtcgg caaaagacaa caatttttta acagctgtag ttagtcaagg
    10561 aaatgaattt ggctttgctt atgccgattt aagtacggga gaattgaaga cggctcggtt
    10621 gcatgatgaa gaagctgttt taaatgaagc ctctgcattg cagacaaaag aactcgtatt
    10681 aggcagtgag atcactgata cccttcgtga acagctaagc gggcgtttag ggctagtctt
    10741 ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc tttttgacaa gcgaactagt
    10801 cgatccgctg gaaaaagaag caaccggcaa actattgact tatttatcgg tgacacaaaa
    10861 gcggagttta gctcatatcc aaaaagcagt agaataccaa ccagatcatt ttttgaaaat
    10921 ggattattat tcaaaattca acctagaatt aagccaatct attcggactg ggaaaaagca
    10981 cggcactctt ctatggttgt tagatgaaac aaaaacagca atgggtggcc gtttactaaa
    11041 acaatggcta gaccgtccgc ttatccaggc aaaacaaatt aaagcaagac aagaaatggt
    11101 cgcttctttg ctagacagct attttgaacg tatcgatttg cagtcagcat taacgaaagt
    11161 ctatgatctg gaacgcctag caggtcgagt agctttcggt aatgtcaatg ggcgagactt
    11221 gatccagctg aaaacttctt tagaacaagt gccaatgatc agagggttga ttgaaggaat
    11281 cgatcaaggt caatggcaaa gtctgctttc agaaatgcag ccgatggacc atctcgtcca
    11341 attgatcgac caagctatcc aagatgatcc gccgttacaa ataacggaag gaaacatcat
    11401 caaagatgga ttcaatgagc agctcgatat gtaccgttct gctatgagaa acggaaagaa
    11461 atggttggca gaacttgaag caaaagagcg tcaagaaaca ggaatcaaga acttaaaaat
    11521 cggatttaac cgcgttttcg gttattatat agaaattaca aaagcaaatc ttggaaatgc
    11581 agaactggag aagtatgaac gaaaacaaac tttagcaaat gcagagcgtt tcatcacacc
    11641 agaactaaaa gaattggaga cacaaatcct cgaagcagaa gaaaaatctg ttgatttaga
    11701 atatcagttg tttttagctg tgagagaaga agtcaaaaag gctatccagc cgctacaagt
    11761 acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt tttgcaacga tcagcgaacg
    11821 ctatcagtat gttcggccag aacttgtcag tgacaagcat cagcttctga tcaaagacgg
    11881 acgacatcct gtagtagaaa aagtattagg acatcaggaa tatatcccta acagcgtaga
    11941 aatggcagaa gacgaaatga tcttgctgat tacaggaccg aatatgtctg gtaaaagtac
    12001 gtatatgcga cagttggcat taactgttct gatggcgcaa atgggctgtt tcgttccggc
    12061 aaaacaagca gttttaccta tttttgatcg gatttttacc cgaattggtg caagtgacga
    12121 cttaattgct ggacaaagta cttttatggt agagatgatg gaagccaatc aagccctgcg
    12181 ttatgctacg ccaaacagtt tgattttgtt tgacgaacta gggcgaggaa cagcaaccta
    12241 tgacggaatg gcgttggccc aagcaatcat cgaatatatc catcgtcatg tacaagcaaa
    12301 aacactcttt tctactcatt atcatgaact gacggtactt gagaaagaac ttccgcagct
    12361 taaaaatgtc catgttggag ccgttgaaaa agacggagaa gttgtcttct tgcataaaat
    12421 gatggatgga cctgcggata aaagctacgg catacatgtg gcaaagatcg caggattgcc
    12481 ttccaatcta ctagaacgag cagcagatat cctgcatacg ctagaaaatg gcgataacgg
    12541 acaccatact gttccagctg aagtagaaga gaaaactaaa acagccaaac aggagcaaat
    12601 aaaaccacct gcagaaaaag taaatcaaga ccatatacaa gaccgtgtaa aagaagaaaa
    12661 ccaacagctt tctttattca gtgaattatc cgaaaatgaa acagaagtca ttgaatcact
    12721 gaagcaaata aatttactag aaatgacgcc aatggatgca ctaaacacat tatatgaatt
    12781 acaaaaacaa ctgtaggaag aaggcggata tcgatggcca aaatccaaga attatctgaa
    12841 cgtttagcta atcagatcgc tgcgggagaa gtagtggagc gaccagcttc cgttgtgaaa
    12901 gaattagtgg aaaatgcaat tgacgccggc agtactcaga ttgatatctt agttgaagaa
    12961 gcgggactgc ggacgatcca agtgattgat aacggagaag gcattttggc agacgacgta
    13021 gagaatgcct tcaagcggca tgcgacaagt aagatccata atcgcgacga tctttttcgc
    13081 atacgaacgc tgggctttcg aggggaagct ttgccaagta ttgcctctgt ttctgaaatg
    13141 actctggaaa cggcgaatca ggaagaaaaa caaggcacct atctgtcgct gaaaggcgga
    13201 gaagttattg agcatcgacc agcaccactg cgtcaaggaa cgaagattac cgtcagcaat
    13261 ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa cactgcacac agaattagcg
    13321 aacatcgggg acatcgtcaa tcgactggca ttgagtcatc catcgattgc ttttcgttta
    13381 gtacatgatg gaaacaaaat gctggcaact gctggaaatg gcgatctgaa acaaacaatt
    13441 gcgggaatct acggattatc taccgcaaaa aaaatgctga aaatcgaagg aaaagatcta
    13501 gattttgagt tatttggcta tgtttcattg cctgaggtga ctcgtgccag taggaattac
    13561 ttatcgacga tcatcaatgg acgttttatc aagaactttt ccttgaataa agcaatcgtt
    13621 gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta ttgctgtatt ggaaatcaaa
    13681 atggacccgc ttttagtaga tgtaaatgtt catccgacga agcaggaagt ccggctgtcg
    13741 aaggaaaaag aactaacaga gctaattagt aacgccattc aaaaagcatt gtctcaagaa
    13801 aatctgattc caagtgcagc ggataatcta cggtttaaga aaaaagtacc tgatcagcct
    13861 aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg aagaagaaaa acctaaaaag
    13921 ccaagtagtt tgaattttga tcgagagaca ggtacctttt tcgtagaaga actaaagaac
    13981 acatttgttg acagaattgt tgataagtct gtggaaaatg ttgaaaacta ttctggtttt
    14041 tctgaagaaa acaccactta tccagaaaaa acagcgaaaa cggtggataa gtttggtgaa
    14101 aatgttgata actttgtaga aaaaacagtg gaaaaatctg aaaagaaaga atttcccatt
    14161 gtggataaca tggagaaaac aaatgaagaa caagcagata aatccgtaga agaggatgaa
    14221 aagatttacg aggaagaatt gacactccat cctgaatttg atttcactca aggaaacgac
    14281 aaaacattag aacaagccat caagaaatta gaaacggaaa aaccagtaca acgttttcct
    14341 cagttggaat attttggaca gatgcacgga acgtatcttt ttgcccaaag tagcgatggg
    14401 ttgtacatca ttgatcagca tgctgcccaa gagcgcatta agtatgaata tttccgggaa
    14461 aaaattgggg aagtatctga tgatctgcaa gaattgcttg ttccatttgt gttagactat
    14521 ccaaacagtg atgctttgaa attaaaagag caaaaacact tgctagaaga tgcagggatt
    14581 tatttagaag aatttggtca aaacagcttt attgtacggg cccatcctac ttggtatccc
    14641 aatggagaag aagaatcgat tattcgggag atgatcgata tgctgctgac aactggaagt
    14701 gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga tgagttgcaa acgatcgatt
    14761 aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc tgctgaagga tctggcacga
    14821 tgcgaaaatc ctttcaattg tccccatggc cgtccagtat tgattcactt taccaattcg
    14881 gatatggaaa ggatgttcaa acgaatccaa gacccgcatt aagtcaaaga gaagagggaa
    14941 aacatgaaga ttgtattagc atcccaatca ccaagaagaa aagagttatt ggctcatatc
    15001 gtttcagaat tcgagattga acctgctgat atcgatgaaa cgccatttcc agacgaacag
    15061 cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac gaactgtctg ggaacaagat
    15121 gaaaaagaag accatttagt gattgccagt gacacgactg ttgttctcaa tcaagagatc
    15181 atgggtaaac cagaagatct ggctgaggct gaattcatgc tgaaaaaact aagcggggaa
    15241 acacatactg tatatacagc tgttgtttta aaaacaaagg aacgagaaga acggatacta
    15301 gcagaagcac atgtcacttt ttatcctttg acagacgaag agatcaaacg ctacttggag
    15361 acgggggatt atgcggataa ggcaggggca tacggcatcc aaaatgctgc gagtgtattc
    15421 gtcaaagaaa tttctggaga ttattacagt atcgtaggat ttccgattgg tgctgtcaat
    15481 caggcactga aaacattcat ttaaacaaaa aagcggacta tctgattttc gattgaaaat
    15541 ccatgatagc ccgttttttt tattgaaaag attacttatc tactctgact aagattttac
    15601 ttcagcttta ccgactgctt cgcccgggcg aacaagtcct gattggtcaa gagaaagtcc
    15661 tgcaactttt tcattattgg tcaaagcaac aataatgtca gttttcttgc cagcttgttc
    15721 tatttctggc aaatccattt cagcaatcag ggtctctggt gtgacaaggt atccttcttt
    15781 cacgaataca ttgaaaggaa caccctttag ttcaacagta tctaatccca tgtgaacaag
    15841 tacttccagc ccctcattag aaagaatacc aatggcatgt tttgtttcaa agacactcgt
    15901 cactttaccg tttactggtg catagacttt gccgtttgac ggttcgacag cataaccgtc
    15961 acccatcatt ttttgtgaga aaactgggtc atttacttca gttatttcct tgatggtccc
    16021 tgcagcgact gcgaataatg tttcttttgt tgcaactgcc gtaccagttg ctgaaacagg
    16081 ttcgaaagac tgtggcgttg ctgttgcgcc atttgctgtt tgaagttgta catcagcagc
    16141 attttcagga acagcaggat caatcttatt caaaattccc tgacgtctaa agatgatcgt
    16201 caagccaaat ggaatgatga cagcaatgat cattgcgatg atgaaaggta cccatgtttc
    16261 tgcttggata gctagtattc ctggcaaacc gcctactccg atcgcgttag ctcgaacacc
    16321 catcaagtta gcaaacatgc cagctaatcc agagccgacc attgcagcga cgaaaggata
    16381 gacgtatttc aaattgattc cgaacatagc aggttcagtt acgcctaagt aacaagaaat
    16441 cattgctgga atcgagattt gttcttcttt tttattccca cgatgtaaga agatgatggc
    16501 taagactgca gagccttggg cgatatttga taaagcaatc atcggccata aattcgttga
    16561 tccgaaatct gcaatcagct gcatatcgat ggcattggtc atatggtgga gaccagtaat
    16621 cactaaagga gcatacagga atccgaatac ggcactaaac aaccagctga tagaagacgt
    16681 caatccagta tttacgatat ttgagatcca gcttccgact gtccagccga taggacctaa
    16741 aataacgtgt gcagctaaaa cagttggtaa caatgaaaac aatggaacaa aaatcatcga
    16801 gatcgactga gggatatatt tgcggaagaa gatttctaga tacgctaaca taaaccctgc
    16861 cagcatggct ggaataactt gcgcttgata accaatcatc tggacttgag caaaaccaaa
    16921 atcccaaaat ggaatatctg ctgcggctgt ggaagctaca ctataagcat ttaataattg
    16981 cggagaaact aacgtaattc ctaaaacgat tcctaatatt tgtgtagtac ccatcttttt
    17041 tgcaatactc caagtgatac ctactggaag aaaatggaaa attgcttcac ccggtaacca
    17101 caaaaaggca ttgacaccat tccaaaactt cgagtgttca acgattgttc caccaagact
    17161 ttcaaactgg ataccttcaa gtacatttct gaatccaagg atcaatccac cgacaatgat
    17221 tgcaggaatc agtggtgtaa agatttctgc taaaacggca atagcacgtt gaacgggatg
    17281 aaggttttgt tttgcagctg cttttccttg ttctttggaa accccttcaa ctccagatac
    17341 tgcaacgaag tcgttataaa aagtagaaac atcgtttcct atgatgactt ggaactgtcc
    17401 tgcattcgtg aacatccctt ttacgctagg tatatcttcg atcgcttttt cgtccgcttt
    17461 tcctggatca ttcaggacaa aacgcatacg agtcgcacaa tgactaactg cagcaatatt
    17521 ttcttttccg ccgatatcat gtaacagctt ctcggcatct gcttgatatt ttgccatatc
    17581 aataaacccc tttctcttaa cttgtctatg caagttgttt cttcatttac tatacttttt
    17641 ttcggagaga attgcaagcg ttttattaaa aagtacgaga gaaaatgata tcgttttaaa
    17701 aagcttaaaa taagcttttc ttaaaaatag gaacattgaa atacaaaaag agctgtagta
    17761 tagtgaagaa atgatggaag aacgagtgag ggaaaaaaca tgaataagtt caatgaaatt
    17821 tttttagatt tggaacagaa aattttagcg aaagaatatc ctccgcattc gcttttaccc
    17881 agtgagaacc aattgatcaa aatttatggt gtttcacgag aaacaattag aaaagcgctg
    17941 aatcttttga ctacggaagg ttatattcaa aaaaaacaag gaaaaggttc gatcgtactg
    18001 gatcggaatc gcttcgactt tcctatttct ggattgacta gctataaaga gctacaggag
    18061 acccaacgta ttccaagtga aacgatcatc catacgttag aagaaacaga agtgacaaaa
    18121 gcattgaacg agatcactgg ctgggagatc ggtgcgcctg tttggcattt gatacgcgaa
    18181 agaaaaatca atggcgaagt agttatatta gacaccgatt atctattaaa ggaaatcgtt
    18241 cctattttaa ctccagcaca agcacaagga tcgatctatg aatattttga aaatgagctt
    18301 tctttgacga ttgcttacgc gcaaaaggag atcacagtag aagaagtaac ggaaatcatg
    18361 aaaacagcga tggatcttca cgatgacact tatgcagtag tagtcagaag cctcgttcat
    18421 ctggaagata cccgctgttt cgagtattct gagtcggttc accgattaga taaattccgt
    18481 ttcgttgagt ttgcccgacg ccgaaaagtg taaatagttc aaaagaaagc aaattgttgg
    18541 catctgtcca ctttggcgct atgataggat cacaaagggg gcatttttaa tggataaacc
    18601 aataagacca gaaaaagaag aactgaaaga aaagttatca cccatggctt atgcagtcac
    18661 acaagaaaat gcaacagaac gaccatttag cgggaaatat gatgattttt acgaaaaggg
    18721 gatctatgta gatatcgtca gcggtgaacc gttattcagc tctgcagaaa aatatgatgc
    18781 aggttgtggc tggccatcat tcagcaaacc aataacgaaa agacaagtgc gcgaaaaagc
    18841 cgatttttct catggcatgc atcgaataga agttcgaagc aaagaagcag attctcatct
    18901 cggacatgtg tttaccgatg gaccagtaga tcaaggtggt ctacgttact gcatcaattc
    18961 tgcctcactg aaattcattc catatgaaaa gatggatgaa ttaggttatg gggaattcaa
    19021 atcacttgta gaataatgga aatacacaaa gagcggtaca atacggggat ttactcgatc
    19081 gtccgctctt tttttattca agcgattgct atttgaaaga aacgctccct agactccttt
    19141 tttcacaagg tgaaatgtgc tacaataaag ggaacgtatg tgtgcaaaag aaagaggact
    19201 agaatatgta tgaatatctg acaggtgtgg ttacctttat caacccctat tacatagtag
    19261 tagaaaccaa tggtatcggc taccagatcg cactaggaaa tccttaccgc tacagtagca
    19321 aactaaatca aaaaataaaa atttatgtcc atcaggtaat acgtgaagat gcgcatcttc
    19381 tgtatggatt tgattcgcta gaagaaaagc aactgttttt gcgattagta agtgtttcag
    19441 gaatcggtcc gaaaagtgcc cttgcaatca tggcaagcga agaccactcc ggtttgattc
    19501 aagcagtaga atccagtgat gtcacttatt tgacgaaatt tccaggagta gggaaaaaga
    19561 cagcacaaca aatgatcttg gatctaaaag gaaaattcgg tgagctgagt atcgatacac
    19621 cttttagtct atttgatgaa gcaaacacaa aagacgctac agctctatct gaagcaatgg
    19681 aagctctctc ggctcttggt tacagtgatc gagaaatcaa acgtgtagaa aaacaattaa
    19741 aagaagtaga taatcagaca acggatgagt atctacgcca agcactcaag ctaatgatga
    19801 aaaaataagg aggagaccaa atgacagatg aatacttgtt atcaccagaa tctggcgaaa
    19861 atgaactgag tttggaaaaa tctcttcgac cacagctgct ttctcaatat atcggacaga
    19921 gaaaagtcaa acaagaactt cagatttaca tcgaagctgc cagaaatcgg gaagaacctt
    19981 tagatcatgt tctgttgtac gggcctcctg gattaggaaa aacgacgatg gccatggtca
    20041 ttgctaatga gatgaccgta aatattcgta cgacgagtgg ccctgctatc gaacgtccgg
    20101 gtgacttagt agcaatcttg aatgaactgg aagcagggga tgtcctgttt atcgatgaga
    20161 tccatcgatt acctagagtg gtagaagaga tgctttattc agctatggaa gatttctatg
    20221 tagatatcat ggtaggacaa gggccaaccg cacatccggt tcatttccct cttcctccct
    20281 ttactttgat tggcgcgacg acacgggcag gtatgctttc tgcaccttta cgagatcgat
    20341 ttggcatcat ttctcatatg gaatattatg acgaacagga tctgcaggag atcgttttac
    20401 gttcttcgga tatttttcaa acagagatca ttgaagaagg ggcattggaa attgctcgcc
    20461 gatcacgagg aacgcctaga atcgctaatc gcttgctcaa acgggttcgt gattttgcgc
    20521 aagtccaagg agacgggaaa atcgatcgtc ctatagcgga tcaggcactt actttgctac
    20581 aggtcgatca ggctggattg gattatgttg accagaaatt gttgcgaaca atgatcgagt
    20641 tatatggcgg cggaccagta gggttaagta cgctttcagt aaacatagga gaagagcgtg
    20701 agacggtaga agatatgtat gaaccgtatc tgatccaaaa aggattttta aagcgtacac
    20761 ctagagggcg aattgctact gcttatgcct atgaacattt tggttacgac tatcatatat
    20821 gatttttgct tttagattgg aggaaaatga atgagtgata caacacgtac gaaacaattg
    20881 atcaaaaacg tattgattga tttgtgccaa acgcaatctt ttcgaaaaat cagcgtccaa
    20941 aaaatcgcaa acgaagcaaa aatcaatcga caaacatttt attatcattt tacagataaa
    21001 tacgatttgc ttcgttgggt ctattgtgag gatgcattgc attatttaga tccacaatgt
    21061 ctttcacttg ataattggga agagcaagca ttgaaaatgc tgaaagcaat tgctgagaag
    21121 aaagaatttt atgcaaatac tgtgctttct gacccagaaa ttttacaaaa agagttttct
    21181 caactgatcc aaaaatcatt tatcaagatt tttgaacaaa tggatgaaga aagacaactt
    21241 tctgcaagag acaaactatt ttatgctcgt tttttttctt acggttgcag tggtgtatta
    21301 gtcaattgga tcacgaatga atatgaagaa acaccgatgg aaatcgctat gcagcttttc
    21361 cgtttagcca aggatacgga attatattct tatcgtcttt atgcaaaaga agagttggaa
    21421 gaacagtaat agaaaaattt tttgttggaa aagcaataat cgctaaaagg agatggggaa
    21481 atgacaaaaa ttttatttgt ttgtttggga aatatttgcc gttcaccgat ggcagaaggc
    21541 ttgctgagaa aacgaatagc attagaagga agggaagcag aattttttgt ggattctgct
    21601 gctacaagca cgtatgaagt tggaaagaca ccgcaccctg gaacgaaaaa aatattaaac
    21661 caagaaaaag tagatatgac gaatatggtt gctcggcaaa tcacacccca cgattttgaa
    21721 acttttgatt ggatcattgg catggatcaa gaaaatgtag aagaactgaa acgaagagca
    21781 ccacgctcag cgcaggggaa aatccatttg tttttaagta gcattcctgg aaaagaaaaa
    21841 gaaaatgttc ccgacccata ctataccaat aactttgatg agacatatca cttgatcaca
    21901 gaaggcctga atcattggtg gaatatttgg attgatgaaa ataaaataac aaataatttg
    21961 taaagttaac gctttctttt taaggcgatg ttagagcatt tccttgtttt tttgtaaaat
    22021 gttaggtaaa ataaaattaa ttaagtaaca ctttcttagc taaaggagga gtgcattcga
    22081 tgaacgaaga gacagtatcg ccaatgaaga agcttacgaa gttagttact catacttcgt
    22141 atcaactttt tgattctttt gtaaatgaca ctgaagaaca tgctaagaat gtacgattgc
    22201 aaggaaagtt tattcttgag ctgcaaaaag ctgctactga acatagtcta gttatcttac
    22261 aagtaagagc aaatcgtttg agtagcaagt atgaaacgat ctcaggctgg gtggcgacaa
    22321 aaacggtcga aaaagaccga gtgatgatcc gcctacaaaa cgaagagcag cagatccgga
    22381 tggttcctgt agaacaaatt gaaaaaataa caatcctttc accagcaggg gaacaggaac
    22441 ggttatcaaa ataatttata ccagatagaa aagcacgtat gagcttgtac catcaatcat
    22501 tactaatcaa aaataaccga atgctttaag ctgatatcag taaaacggaa cgttttagga
    22561 tgagtatcat agcatgacag cattcggttg attagttgaa tcgattggat ggtacaagtt
    22621 tttttgataa aaaaaggtcc tgctagccgg ggagctagca ggataaggag ttaaaaatga
    22681 aaaagtgttg ttagggttgt ttgttggtat gcttatataa tagtcattag ttgtgaatat
    22741 tttgtgacga aagttttcga aagttgttaa gtttt
//
LOCUS       DAHEYX010000021        22495 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHEYX010000021 DAHEYX010000000
VERSION     DAHEYX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.021"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(43..654)
                     /locus_tag="MB219_000565"
     CDS             complement(43..654)
                     /locus_tag="MB219_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCC8426301.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(777..902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(984..1700)
                     /locus_tag="MB219_000566"
     CDS             complement(984..1700)
                     /locus_tag="MB219_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCC8426302.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1706..2275)
                     /gene="scpB"
                     /locus_tag="MB219_000567"
     CDS             complement(1706..2275)
                     /gene="scpB"
                     /locus_tag="MB219_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCC8426303.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2259..3056)
                     /locus_tag="MB219_000568"
     CDS             complement(2259..3056)
                     /locus_tag="MB219_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCC8426304.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3026..3397)
                     /locus_tag="MB219_000569"
     CDS             complement(3026..3397)
                     /locus_tag="MB219_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCC8426305.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3416..4303)
                     /gene="xerD"
                     /locus_tag="MB219_000570"
     CDS             complement(3416..4303)
                     /gene="xerD"
                     /locus_tag="MB219_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCC8426306.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4399..4872)
                     /locus_tag="MB219_000571"
     CDS             complement(4399..4872)
                     /locus_tag="MB219_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCC8426307.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4977..5840)
                     /locus_tag="MB219_000572"
     CDS             complement(4977..5840)
                     /locus_tag="MB219_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCC8426308.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6036..8135)
                     /locus_tag="MB219_000573"
     CDS             complement(6036..8135)
                     /locus_tag="MB219_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCC8426309.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8355..9464)
                     /gene="rpoD"
                     /locus_tag="MB219_000574"
     CDS             complement(8355..9464)
                     /gene="rpoD"
                     /locus_tag="MB219_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCC8426310.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9488..11329)
                     /locus_tag="MB219_000575"
     CDS             complement(9488..11329)
                     /locus_tag="MB219_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCC8426311.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11511..13826)
                     /locus_tag="MB219_000576"
     CDS             complement(11511..13826)
                     /locus_tag="MB219_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCC8426312.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14011..14871)
                     /locus_tag="MB219_000577"
     CDS             complement(14011..14871)
                     /locus_tag="MB219_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCC8426313.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14930..15562)
                     /locus_tag="MB219_000578"
     CDS             complement(14930..15562)
                     /locus_tag="MB219_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCC8426314.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            15729..16490
                     /locus_tag="MB219_000579"
     CDS             15729..16490
                     /locus_tag="MB219_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8426315.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16516..19107
                     /gene="mprF"
                     /locus_tag="MB219_000580"
     CDS             16516..19107
                     /gene="mprF"
                     /locus_tag="MB219_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCC8426316.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19295..20482)
                     /gene="tuf"
                     /locus_tag="MB219_000581"
     CDS             complement(19295..20482)
                     /gene="tuf"
                     /locus_tag="MB219_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCC8426317.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLTE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20850..21206
                     /locus_tag="MB219_000582"
     CDS             20850..21206
                     /locus_tag="MB219_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCC8426318.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21447..22451)
                     /locus_tag="MB219_000583"
     CDS             complement(21447..22451)
                     /locus_tag="MB219_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCC8426319.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         6151 a         4724 c         3912 g         7708 t
ORIGIN      
        1 tccaaaaaaa aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg
       61 acgttgttcg aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac
      121 taaaaagaca gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag
      181 tacatagttt gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa
      241 gtaaagaggc gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt
      301 tcctgtaatg acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa
      361 ataataaatc gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa
      421 attatccgga gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc
      481 aactccggcc gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc
      541 gatcttcaaa aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc
      601 gatcgcagca aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa
      661 tcctcctgtt aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac
      721 tctattttcc agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct
      781 atcttcttta tccagactat actgtcggta tcggaatttc accgattcag ctattttttt
      841 gcaaaatagg tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga
      901 accttatata atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc
      961 taacacttat aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt
     1021 ttgttcagtt cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt
     1081 tcacgtttta gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga
     1141 ttacgtcctt cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat
     1201 aaaatttgga atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa
     1261 ggtgcaagca tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact
     1321 tcatgttttg gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc
     1381 cccgaagtat cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa
     1441 agatccgtaa caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt
     1501 ttgtacaata aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca
     1561 gtgtcatgtt tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca
     1621 cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt
     1681 actttctgca atctctccat tacaattatt ctcctttatt cattccatca aagaatagat
     1741 ctggtgacac gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta
     1801 atgatttcaa accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac
     1861 ctggtccttc tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg
     1921 ctcctgaaac ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat
     1981 aagcgatgat tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg
     2041 catatcgctt aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg
     2101 tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca
     2161 ctaaacttgt gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga
     2221 tcccttcctc gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg
     2281 gtatctccta ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg
     2341 atctgccctt ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta
     2401 gaaggtgact caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct
     2461 tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact
     2521 ttttgagaaa atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt
     2581 ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt
     2641 tcttttgtgt aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat
     2701 ttatatttac ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat
     2761 aattcttcat cgtctgtgac gaattcttgg acaggaagca atgtcttgct tttgattgcc
     2821 atcaatgtag cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt
     2881 gtatggatat aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg
     2941 atttccattt gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat
     3001 ttaatattga tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc
     3061 caatgtaagg ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta
     3121 attcatctaa catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt
     3181 catggatgat cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg
     3241 ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa
     3301 tctccttcaa caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag
     3361 caattttacg gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag
     3421 cccttggaaa atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa
     3481 tctgagtcgt agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc
     3541 cattttctag cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt
     3601 aaatcccagc ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac
     3661 ctttcccatg attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac
     3721 gtgcttcgtc taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc
     3781 gctctttatc tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca
     3841 atttcaatcc aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg
     3901 cacgatctct tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt
     3961 ctgtcagagt caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca
     4021 tcggatcatg atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg
     4081 agatcatccg agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac
     4141 ggatgactaa aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat
     4201 gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt
     4261 caatattcaa gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc
     4321 ctctctatca tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag
     4381 aaaaagccgt ctagacactc agacggactg gtttcttttt tctttttcat agcattcctg
     4441 acagattcca tggaacgtta gccgatgatc ttttactaaa aaatgataac gttcttctac
     4501 tacttgctcc acttcaagta aaagatcctc ttctacttcc tcgatcgtgc cgcagttcaa
     4561 gcatagtaaa tgatgatgga aatgtttggc gccttctttt ctcaaatcat aacgggctaa
     4621 tccatcatta aaacttactt tatcgacaat tttcaattct gtcaagattt caagggttcg
     4681 ataaacagta gcaagtccaa tttccggact cttttttttc actaaaaaga agatttcttc
     4741 tgcagataaa tgctctttct cattttccaa aagcacaagc agagtagctt ctctttgcgg
     4801 tgtcagctta aaccccgctt catgtagctg ctgttttgtt tttttcatcg ccgaaatagc
     4861 attcatgacc atttactcaa ccccaacaat ttataataat tacaaataaa aaaagatata
     4921 acactatgtt aattaaaatt attataagct agttaagaaa aacatgcaag ggattctcaa
     4981 ttagattttt tgactagttt tgtcacaccg ttttcttgtg taatcaatcc ttgtttcaaa
     5041 agattaccta atgcgcgctt aaatgctcct ttgctgatac caaaagcttt catgatttct
     5101 tctggatccg atttatctgt aaagttgatt tgctgatctg cagaacgttc taaaatggtt
     5161 aaaatcattt gtgcatcatc tgatatagct tcatatgctc ttggcttcaa tgaaagattc
     5221 agtacaccgt ctggtcggac accaatcact cgacctttta tctgctcgcc tagacgaggt
     5281 tcattatatc gttcagaagg atggataaac ccaagataga aatcttccgt taaaacgtac
     5341 gtacctgtta atttcaagcg gtatgctgtt cctatcacat ctttgttttt catttcttct
     5401 gttccatatt tgcttaaaga tttgaatatt ttttcatctg ctaaactgcc ccaaatacgg
     5461 tcttttgcat caactctaag ggaaatcatc aagcgatccc cttttttagg ccatagctct
     5521 gtcattgtcg gcaattcatc aagagatacg acaaagtctt tatctggaag accgatatcg
     5581 acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc caaaaccgta atgaccgata
     5641 cggcttttag gaatttgtgt agtgatccga ttttcatgtt tttgattttg gtaaccgaat
     5701 ccttcaactg cttcaccgat tttgtgttca ccttctgatt tgcttaagcc aaaggtttgt
     5761 ccgttttttt ggacaaagta gtttttttca ttttcatcaa taataatacc tgtaaatata
     5821 gttgctaata aatcgttcat ttttttctcc ctctatagtg aaaggttttc ttaatacatt
     5881 tctttgctat cataacataa tgttatctac ttgaatagct gagccttggt ttggaaaggt
     5941 gaaaaaagga gtatgacaac agttgtgtca tactcccctt tttcgaataa acggtgttaa
     6001 acggcaggct cacagcctct ttcatttgcc taaaattaat tgctcccatt tcctccgaac
     6061 catccagttt tacagttgat ggttacgatg taaaatggca aagcactttc tccaatgcct
     6121 ttcatcaatg cagattttgt tgaagtggca tatagattat aatagccttc cccattttcg
     6181 attctggctt tttctaatct gtaacctcct tcaacgatat atccacgttg gatacatgta
     6241 gcaatcactt tgctttggac acctggtgcc cacgtccatg ctggattaga tgcatcggca
     6301 atatcacttg ctctggcaga tttgattggc tgattcgctg agtttgaaga ggaaggggtc
     6361 gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag cttgttgagc ggcttgagct
     6421 gcagctgctg cttgagcggc ttgttttgct tgttcttcag ctgctttttt cgcttcttct
     6481 gcttttttct tttcctcttc ttcttttttc tctttatcag tgagcatctt atctacttgt
     6541 ttcaattgtt cattcaactg agcagataga tctgtatttt tcactttttc tactgcttct
     6601 tttgccgtct tgtattggtc gcgtgttgct gaatccacta cttttccgtc tttaaagact
     6661 atagctgttt tttctttagc tgtttgcaat tcagcatatt gatttttagc ttctgtttgt
     6721 gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt cgattgcttg gatctcttta
     6781 tctgctttca acttgatgtc atctgctaga tgatcatcga cgatggcagg tgctatgaac
     6841 cactcattca ctttttggat atctcggatc ttagattgga tatcttggta agttgtttct
     6901 aactctgaat atcctttttc attcttaaga ctggataaat cattttttag ttttgataaa
     6961 tcttgatgaa tcatatctgc acgaataaat tgatggtgat catctgtata aaaggcagca
     7021 agtttctcat gaagttcttc catttgttgc tgtctttttg cttctgctgc tacttcggct
     7081 tctacttttt gatgatggtc ataataagcc cagcttcctc caccgagtag aagtactgct
     7141 gctaaaacga gataaggtac tttttttgat tttttctttt gcgctttatc tggatctgtt
     7201 ttttctaagt ttgattcttg atgtgttttt tcagtatctg gcacgattgt tattgatttc
     7261 gcttctgctt caaacagctt aggctggttc tcttcttttt ctatttttgc ttcaagatca
     7321 gtaccctctt tgcttacaga ttgattttca gcatcggatt cactatttat ttcatcgggc
     7381 tctgttgact ttttgtctgt gttttcgagt atttcttttt tctctgcttc cggcagttta
     7441 ttcgtttttt ccttttctaa ttgtttttca gtaaatactt cagttttttc tgtgatatcc
     7501 gtttttacgt cttttatttt tactgtttct ttacctgtgg cgttttctct atcagagtct
     7561 ttttcagttt cttgttcctt ttctggctct actttgtttt cctgtgcaat tttttcactg
     7621 atcattttga gcagctcttt cgcgtgctct tcttttgtat cttcacgatg tgctttgata
     7681 taagcggata gtatcgaatt ttcttcctca ttatcgacga tttctttcag ttcagctact
     7741 cttctttttt gttttgattc aagcgtttct tcttgattct ttcctgattc ttccgaagtc
     7801 tcatgaacag ttgctttctt ctgttcattt ttctctatat tagttacttg atcgtgagca
     7861 ggtggttctt cgtcttctgg ttctgctttt tcgaataatt ccattacaga ttctagaggt
     7921 aaatctttca attccgacca ttcgattgga tcgtttaatt ctgtatcagg aaagactgca
     7981 tcttcagttg atttatcctg catagctgca tctgtatttg tttcttcaat tgattctttt
     8041 atttgctgat cttctgcagt tatttgatga ccgcagtctg gacagatagt attctgctcc
     8101 atttttttcg atccgcaatt tggacatttt ttcatcactt agcgatcggc tcctttacta
     8161 ggtttacgct accatttaat ctatcatatt ctatacgtca cgccaatagc ttatttttag
     8221 gatttactta aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg
     8281 tagtctcaag ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg
     8341 tcaatggtcc acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc
     8401 aattttctta atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaacact
     8461 ttccctactt cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg
     8521 acattctctt cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac
     8581 tcataagctg cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag
     8641 tgagagtcat cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc
     8701 aagatttctc ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta
     8761 ggttctcttc caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt
     8821 tcaaccatat gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg
     8881 gcttgacgaa tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat
     8941 ttttcaactg ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg
     9001 cctcgtccta cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga
     9061 cgctgtttcg cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc
     9121 gcagttaaaa gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt
     9181 tttacaccgg ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca
     9241 ctcttcaagc tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct
     9301 tcaatctttt ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc
     9361 tgattggata aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct
     9421 gctactgctg cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct
     9481 tgagtagcta tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt
     9541 caagctcaag ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg
     9601 ttatttcatc agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg
     9661 gcgaactttc ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt
     9721 catcttgcaa aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg
     9781 catcgaataa aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt
     9841 ttaaaatatt acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct
     9901 tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt
     9961 gttgactttc ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt
    10021 gtttttgtat cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat
    10081 cttgttcgat tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga
    10141 tcttgtcttt ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa
    10201 agactgtttc gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga
    10261 gatattcatc aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt
    10321 tttgccgaag caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat
    10381 agcaaaagac caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac
    10441 ttgtccccat agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca
    10501 taaacccttc gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa
    10561 gattaaataa aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg
    10621 gctgatcttt ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct
    10681 ggccttgaga attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc
    10741 catcatcatg cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat
    10801 tcttgaatac ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt
    10861 caattaattc taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt
    10921 ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt
    10981 tccccgtagc agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta
    11041 acggtatctg ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta
    11101 gttcttgaag aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct
    11161 gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca
    11221 agtaattttt accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg
    11281 tttgactgcg tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc
    11341 ctgctctatt gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa
    11401 aatcgcacta actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta
    11461 tcatatttca ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct
    11521 actcttttca gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata
    11581 tttacgagca tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc
    11641 aaaattccat ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg
    11701 aagatgcgca atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca
    11761 atcagcaagt aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca
    11821 agtagataca ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata
    11881 agtgctgtag gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag
    11941 aaggaaggat acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga
    12001 aatccaattg ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata
    12061 aaaaagatgc ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact
    12121 tctggcaatg tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct
    12181 ccatcacctt ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc
    12241 acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat
    12301 ttcttttgct gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact
    12361 tccatttctt cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca
    12421 cgtctagcga tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca
    12481 ccttcctcat gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt
    12541 tcaaacagag caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga
    12601 gcgatcccca acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga
    12661 agccgatctt cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga
    12721 aattccatcg cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac
    12781 cgattagaaa gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt
    12841 tctgccagat acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct
    12901 tctacccgct ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat
    12961 gtatcgacat gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt
    13021 gccagtttgg ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga
    13081 agcatgccta acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg
    13141 atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat
    13201 ttggatactt tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc
    13261 tcgctagtca agcggacagc ataattatct gcacctactc ggataacttc agccatgaca
    13321 gaaccgcttg tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa
    13381 tcagattcgc ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa
    13441 tcggctggta tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta
    13501 gtagaaatcg attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc
    13561 gataatttcc gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg
    13621 acgacaataa ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc
    13681 ccaataccaa agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt
    13741 gtactttttg atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact
    13801 acctcttgct gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa
    13861 ataatcacgt gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat
    13921 ttatagaaaa acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac
    13981 ccgtgtgctg tttttattat caatctatct ttacttatat tgttcctcca aacggctcat
    14041 ccaccaacct agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac
    14101 tcccaatccc gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa
    14161 aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa
    14221 tccaagaaca caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc
    14281 tttaaatccg tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct
    14341 caatcctggg acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa
    14401 attctgatcg acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag
    14461 aacaaaagta acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc
    14521 tttccataac gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt
    14581 ttcatacgca cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc
    14641 gcctagacca acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc
    14701 taaaaaagaa atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac
    14761 tcctggcaag ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc
    14821 tttggttgtg taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc
    14881 actccttctt tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga
    14941 ctttttcata ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt
    15001 tacctccatt atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc
    15061 ggtattgttt cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt
    15121 ctgctaattc tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc
    15181 caaaattata tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta
    15241 tcatttccgc caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct
    15301 cagcttctcc atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt
    15361 cggtcaatat tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag
    15421 ctgttgttat ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc
    15481 ctaaaaacaa caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa
    15541 tagataatcg ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta
    15601 ctatttcttt atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa
    15661 tgaattttcc tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca
    15721 attgcccaat gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag
    15781 aaaagaaata tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag
    15841 gcttccctct ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg
    15901 tccctgtact cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt
    15961 cgacaaacga agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca
    16021 tgttgctgga aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag
    16081 cactggtatt tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa
    16141 acacgcttgt taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat
    16201 gggtatggtt actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac
    16261 ttttgctgca tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt
    16321 tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa
    16381 ctataccaaa agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag
    16441 atcctgaacg cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa
    16501 tagaaggtga tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg
    16561 ttcaaaacaa tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc
    16621 ggtaaaactc tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa
    16681 agcggactaa tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt
    16741 attttgaatc ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg
    16801 ctgatcaata cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc
    16861 tctgaactat atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt
    16921 ttcttgtttt tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc
    16981 ttcacgccag ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta
    17041 tattttcctg tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc
    17101 tcacttaaaa ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg
    17161 tttagtttcc tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt
    17221 ccattattca ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg
    17281 agtttcgatg taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc
    17341 gttgtgtgga tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc
    17401 gtctttttct tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa
    17461 caattagcaa cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc
    17521 atgctagtac tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt
    17581 ttgaacccat tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt
    17641 ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc
    17701 ttcttgattg ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg
    17761 gttttcttgt ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa
    17821 caattgatct attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc
    17881 ttgctttaca tcgttattgg ggtatacaat ctgccagatt tccctcatcg gcgtcaccat
    17941 tttatttcct ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc
    18001 atcgctgtta gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag
    18061 attggcgaag cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac
    18121 tccgatagtc aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt
    18181 gaaccaactg ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat
    18241 ccttctggta agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat
    18301 cgactatgct atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat
    18361 gaattcggct acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt
    18421 acaacttcag ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa
    18481 ggatttacat ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa
    18541 aatatctctg ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc
    18601 tctgaagatt atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt
    18661 gtcgcttttg caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg
    18721 atgcgatatg attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg
    18781 tttgcctata tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca
    18841 aatgtcggaa cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag
    18901 tttggttctc atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca
    18961 aactgggtct cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt
    19021 gctttgctga ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg
    19081 aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga
    19141 tgaataaaag agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa
    19201 ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac
    19261 atttttcatt accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac
    19321 ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga
    19381 ttggatggat caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat
    19441 cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat
    19501 agttgttgaa gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg
    19561 ctttgaattt tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga
    19621 tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat
    19681 ctaacgtttt acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga
    19741 tccctacgat ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg
    19801 caacagttcc tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat
    19861 ctgtatcacg ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag
    19921 cagcttcagc atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag
    19981 tgtcatcgcc tgggaaacca tattcggtca ataactcgcg aacttccatt tctaccaaat
    20041 cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta
    20101 caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg
    20161 cagaaacaac taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat
    20221 agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta
    20281 cgtgtgcggt attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag
    20341 catagtcttg aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta
    20401 aagtagtttt cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc
    20461 tgtcataatg ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa
    20521 atttcagctc tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc
    20581 gacttacttt ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa
    20641 ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg
    20701 ttttttgaga aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat
    20761 gaggaaaagt ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt
    20821 cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt
    20881 taggcatatt tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt
    20941 attatacaaa aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga
    21001 aattcacgac ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg
    21061 tgacgcttcg cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag
    21121 tcaatcctcg taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa
    21181 atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag
    21241 gctgggacag aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt
    21301 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttttt
    21361 gttatattcg tcttttccga tccttctctt gacacaattc atttaactga tcactggtac
    21421 ttgaacacct aatattctag tcatttttat aaacgattca atacttcatc cacatttttg
    21481 atcatcacgg atatcgtacc atccggatta ttcacaaatt caatcaaatc tgggttgcga
    21541 tatacatcta ctggtacgat cagttcgatc ccgtttgata gcttgaattt ttgttttcca
    21601 aattttttct cagaaatttc ttgtacttct tttacaggag gtgcttctgg gacaaatcct
    21661 tgctcttgta cttcttcttt aaaagccatt tttgccgtga tgttttcctt gaagactttt
    21721 tccgcaatca tctcatgatc caaccgacct ttttcttcta tcgtatcata gacagcttct
    21781 ttcaaatcag ccatgatatc aaattcttct gtctcaaatt tttttccaag atgcttggct
    21841 actttttgga tcacttttac attttcttct aaagaaggtg caggaacact ttcgatcact
    21901 cttccagaaa aataaagctc ttttttgcca gaaaactcat attttttttc aattaattcg
    21961 taggtccaat cagtcaaatt cacagcgaat gcttcatctg ctttttgtgt tttcgcccct
    22021 agaattgctc gattcaaaat caagcggttg tcgatccctg cttcgtctgc ttcaacaaag
    22081 tgagtgtaag cctcacgata attgactttc aataaagata agtacatcac tgtatctaat
    22141 tcatacaaga caacaaatac atctgcacta ggtgcttctt cactttcttg gtagatatcg
    22201 aaccaccgct gaacgattgc ttcacttgct tccacaaaat tttcttgagc gattttaagt
    22261 gattcaacga tcatgctttc ttctgctagt tgtccagttt ttgtttgagc agaagagatt
    22321 ttttggatct tcttttgaag aaaatcacga atatattctt ttgtcagatc cagctctttt
    22381 tgtgaaaaga ctggagaacc agtttctctg tcaataatat gcagaattgc tttttttaaa
    22441 tagatatcca ttttctcatt cccttctgtc ttagtttacg gaaaaaaaat caaaa
//
LOCUS       DAHEYX010000022        22450 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHEYX010000022 DAHEYX010000000
VERSION     DAHEYX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.022"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1184
                     /gene="tuf"
                     /locus_tag="MB219_000584"
     CDS             <1..1184
                     /gene="tuf"
                     /locus_tag="MB219_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCC8426320.1"
                     /translation="KEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQAMA
                     YDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGAIL
                     VVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTEYE
                     FPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDVFS
                     ITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNIGA
                     LLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYFRT
                     TDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTEIK
                     A"
     gene            1591..1899
                     /gene="rpsJ"
                     /locus_tag="MB219_000585"
     CDS             1591..1899
                     /gene="rpsJ"
                     /locus_tag="MB219_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCC8426321.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            1930..2559
                     /gene="rplC"
                     /locus_tag="MB219_000586"
     CDS             1930..2559
                     /gene="rplC"
                     /locus_tag="MB219_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCC8426322.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            2587..3210
                     /gene="rplD"
                     /locus_tag="MB219_000587"
     CDS             2587..3210
                     /gene="rplD"
                     /locus_tag="MB219_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCC8426323.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            3210..3500
                     /gene="rplW"
                     /locus_tag="MB219_000588"
     CDS             3210..3500
                     /gene="rplW"
                     /locus_tag="MB219_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCC8426324.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            3539..4372
                     /gene="rplB"
                     /locus_tag="MB219_000589"
     CDS             3539..4372
                     /gene="rplB"
                     /locus_tag="MB219_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCC8426325.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            4415..4693
                     /gene="rpsS"
                     /locus_tag="MB219_000590"
     CDS             4415..4693
                     /gene="rpsS"
                     /locus_tag="MB219_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCC8426326.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            4716..5063
                     /gene="rplV"
                     /locus_tag="MB219_000591"
     CDS             4716..5063
                     /gene="rplV"
                     /locus_tag="MB219_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCC8426327.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            5077..5733
                     /gene="rpsC"
                     /locus_tag="MB219_000592"
     CDS             5077..5733
                     /gene="rpsC"
                     /locus_tag="MB219_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCC8426328.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            5736..6170
                     /gene="rplP"
                     /locus_tag="MB219_000593"
     CDS             5736..6170
                     /gene="rplP"
                     /locus_tag="MB219_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCC8426329.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            6160..6348
                     /gene="rpmC"
                     /locus_tag="MB219_000594"
     CDS             6160..6348
                     /gene="rpmC"
                     /locus_tag="MB219_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCC8426330.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            6373..6639
                     /gene="rpsQ"
                     /locus_tag="MB219_000595"
     CDS             6373..6639
                     /gene="rpsQ"
                     /locus_tag="MB219_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCC8426331.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            6697..7065
                     /gene="rplN"
                     /locus_tag="MB219_000596"
     CDS             6697..7065
                     /gene="rplN"
                     /locus_tag="MB219_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCC8426332.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            7102..7410
                     /gene="rplX"
                     /locus_tag="MB219_000597"
     CDS             7102..7410
                     /gene="rplX"
                     /locus_tag="MB219_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCC8426333.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            7438..7977
                     /gene="rplE"
                     /locus_tag="MB219_000598"
     CDS             7438..7977
                     /gene="rplE"
                     /locus_tag="MB219_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCC8426334.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            7996..8181
                     /locus_tag="MB219_000599"
     CDS             7996..8181
                     /locus_tag="MB219_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCC8426335.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            8217..8615
                     /gene="rpsH"
                     /locus_tag="MB219_000600"
     CDS             8217..8615
                     /gene="rpsH"
                     /locus_tag="MB219_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCC8426336.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            8647..9183
                     /gene="rplF"
                     /locus_tag="MB219_000601"
     CDS             8647..9183
                     /gene="rplF"
                     /locus_tag="MB219_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCC8426337.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            9344..9700
                     /gene="rplR"
                     /locus_tag="MB219_000602"
     CDS             9344..9700
                     /gene="rplR"
                     /locus_tag="MB219_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCC8426338.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            9721..10221
                     /gene="rpsE"
                     /locus_tag="MB219_000603"
     CDS             9721..10221
                     /gene="rpsE"
                     /locus_tag="MB219_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCC8426339.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            10236..10415
                     /gene="rpmD"
                     /locus_tag="MB219_000604"
     CDS             10236..10415
                     /gene="rpmD"
                     /locus_tag="MB219_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCC8426340.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            10459..10899
                     /gene="rplO"
                     /locus_tag="MB219_000605"
     CDS             10459..10899
                     /gene="rplO"
                     /locus_tag="MB219_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCC8426341.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            10899..12194
                     /gene="secY"
                     /locus_tag="MB219_000606"
     CDS             10899..12194
                     /gene="secY"
                     /locus_tag="MB219_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCC8426342.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            12256..12903
                     /locus_tag="MB219_000607"
     CDS             12256..12903
                     /locus_tag="MB219_000607"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCC8426343.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            13094..13312
                     /gene="infA"
                     /locus_tag="MB219_000608"
     CDS             13094..13312
                     /gene="infA"
                     /locus_tag="MB219_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCC8426344.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            13345..13461
                     /gene="rpmJ"
                     /locus_tag="MB219_000609"
     CDS             13345..13461
                     /gene="rpmJ"
                     /locus_tag="MB219_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCC8426345.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            13479..13844
                     /gene="rpsM"
                     /locus_tag="MB219_000610"
     CDS             13479..13844
                     /gene="rpsM"
                     /locus_tag="MB219_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCC8426346.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            13872..14261
                     /gene="rpsK"
                     /locus_tag="MB219_000611"
     CDS             13872..14261
                     /gene="rpsK"
                     /locus_tag="MB219_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCC8426347.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            14341..15279
                     /locus_tag="MB219_000612"
     CDS             14341..15279
                     /locus_tag="MB219_000612"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCC8426348.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            15307..15687
                     /gene="rplQ"
                     /locus_tag="MB219_000613"
     CDS             15307..15687
                     /gene="rplQ"
                     /locus_tag="MB219_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCC8426349.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            15958..16797
                     /locus_tag="MB219_000614"
     CDS             15958..16797
                     /locus_tag="MB219_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCC8426350.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            16773..17642
                     /locus_tag="MB219_000615"
     CDS             16773..17642
                     /locus_tag="MB219_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCC8426351.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            17632..18429
                     /locus_tag="MB219_000616"
     CDS             17632..18429
                     /locus_tag="MB219_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCC8426352.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            18632..19378
                     /gene="truA"
                     /locus_tag="MB219_000617"
     CDS             18632..19378
                     /gene="truA"
                     /locus_tag="MB219_000617"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCC8426353.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(19711..22380)
                     /locus_tag="MB219_000618"
     CDS             complement(19711..22380)
                     /locus_tag="MB219_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCC8426354.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         7432 a         4134 c         4843 g         6041 t
ORIGIN      
        1 caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg
       61 accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg
      121 gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca
      181 caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact
      241 gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag
      301 ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat
      361 tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg
      421 atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg
      481 aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg
      541 acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa
      601 ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta
      661 ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg
      721 aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa
      781 tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg
      841 gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac
      901 ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata
      961 ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg
     1021 ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag
     1081 ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg
     1141 gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa
     1201 agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc
     1261 cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa
     1321 acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt
     1381 acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact
     1441 ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg
     1501 acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta
     1561 cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta
     1621 aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa
     1681 agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca
     1741 atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac
     1801 aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta
     1861 gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga
     1921 ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact
     1981 gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa
     2041 gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt
     2101 gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag
     2161 cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt
     2221 acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa
     2281 ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct
     2341 cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac
     2401 aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta
     2461 cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa
     2521 tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac
     2581 taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca
     2641 cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta
     2701 atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc
     2761 ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc
     2821 cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc
     2881 tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt
     2941 gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa
     3001 ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa
     3061 ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct
     3121 gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct
     3181 cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag
     3241 tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca
     3301 ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca
     3361 aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat
     3421 atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat
     3481 tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt
     3541 ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt
     3601 tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc
     3661 tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca
     3721 atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat
     3781 cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa
     3841 agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga
     3901 agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat
     3961 ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag
     4021 tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt
     4081 tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga
     4141 attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg
     4201 tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat
     4261 cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa
     4321 caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg
     4381 accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc
     4441 ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa
     4501 agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg gatataccat
     4561 cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca
     4621 caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa
     4681 aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta
     4741 aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta
     4801 tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg
     4861 ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact
     4921 tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac
     4981 gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca
     5041 cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt
     5101 ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat
     5161 gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat
     5221 gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac
     5281 acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa
     5341 gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca
     5401 gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct
     5461 ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc
     5521 aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca
     5581 gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca
     5641 gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca
     5701 actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac
     5761 accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat
     5821 tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag
     5881 ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt
     5941 tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg
     6001 cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg
     6061 ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa
     6121 ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga
     6181 gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat
     6241 cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt
     6301 aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag
     6361 gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt
     6421 tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc
     6481 tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca
     6541 aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc
     6601 cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat
     6661 agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta
     6721 aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct
     6781 ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca
     6841 ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga
     6901 gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt
     6961 gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa
     7021 aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat
     7081 caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact
     7141 ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt
     7201 attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa
     7261 ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct
     7321 actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct
     7381 aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg
     7441 aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac
     7501 tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt
     7561 gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact
     7621 ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa
     7681 ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac
     7741 aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc
     7801 tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta
     7861 gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac
     7921 tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta
     7981 aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa
     8041 ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt
     8101 aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc
     8161 gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg
     8221 tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac
     8281 atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac
     8341 gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg
     8401 ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta
     8461 aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag
     8521 gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa
     8581 atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt
     8641 ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact
     8701 caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca
     8761 gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt
     8821 aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt
     8881 gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta
     8941 caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca
     9001 gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa
     9061 gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa
     9121 ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa
     9181 taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt
     9241 taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta
     9301 ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga
     9361 taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc
     9421 tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga
     9481 tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg
     9541 aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa
     9601 aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc
     9661 tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc
     9721 atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt
     9781 gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt
     9841 gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc
     9901 cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca
     9961 atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta
    10021 gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta
    10081 gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca
    10141 gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca
    10201 gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa
    10261 aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta
    10321 caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata
    10381 ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat
    10441 taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa
    10501 gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga
    10561 caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa
    10621 acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat
    10681 gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt
    10741 gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat
    10801 ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa
    10861 gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg
    10921 cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat
    10981 ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt
    11041 cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt
    11101 ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc
    11161 aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa
    11221 aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta
    11281 tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa
    11341 cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag
    11401 aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca
    11461 tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc
    11521 cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga
    11581 tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga
    11641 aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag
    11701 ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat
    11761 tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata
    11821 atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg
    11881 cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata
    11941 taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt
    12001 taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa
    12061 tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg
    12121 gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg
    12181 gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta
    12241 ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca
    12301 caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc
    12361 cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa
    12421 ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca
    12481 gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct
    12541 ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt
    12601 ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca
    12661 acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg
    12721 catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc
    12781 aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact
    12841 attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa
    12901 tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat
    12961 tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat
    13021 tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa
    13081 ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac
    13141 tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt
    13201 ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga
    13261 attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc
    13321 cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa
    13381 cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa
    13441 cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag
    13501 atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca
    13561 ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat
    13621 taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg
    13681 atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg
    13741 gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta
    13801 ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag
    13861 gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag
    13921 aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata
    13981 ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa
    14041 aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac
    14101 acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa
    14161 ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc
    14221 ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga
    14281 gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga
    14341 atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag
    14401 ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt
    14461 attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg
    14521 catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt
    14581 aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc
    14641 ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac
    14701 aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg
    14761 aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc
    14821 gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac
    14881 acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt
    14941 tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat
    15001 atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa
    15061 acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct
    15121 tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag
    15181 cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa
    15241 ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa
    15301 accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt
    15361 gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa
    15421 gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca
    15481 cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag
    15541 gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca
    15601 gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca
    15661 ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt
    15721 cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc
    15781 aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt
    15841 acctgtcatg acagatatgt ttgaaggcat aaaaaacatt ttaagcgaga aataattgaa
    15901 aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg agtaatcatg
    15961 gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga tcttcgcccc
    16021 gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat tattggacac
    16081 aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct gccagaatca
    16141 gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac gatccgacag
    16201 atggttggaa tgatttttca aaatccagac aatcagtttg ttggttcgac agtagaagat
    16261 gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct agtgcgtgtg
    16321 aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc tgctcgttta
    16381 tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg acctgatatc
    16441 attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga agtgatctct
    16501 acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac tcatgatatt
    16561 gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact tgtccgtgaa
    16621 ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg tctagatcta
    16681 ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa
    16741 tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa caagtagact
    16801 tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc aatatgacga
    16861 tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa tcaaccttac
    16921 ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt ggagaacgag
    16981 atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa gttggcatcg
    17041 tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac attgctttcg
    17101 gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa gaaacattag
    17161 aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt tcaggagggc
    17221 aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta cttgtattgg
    17281 atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag atgttttggc
    17341 gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg gatgatgtag
    17401 ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat agcggggagc
    17461 cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc gtacctactg
    17521 ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc ttgcctttga
    17581 cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc catgatgaat
    17641 aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt ggatccccga
    17701 gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa taattgggaa
    17761 acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa agtagatttg
    17821 aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac tgtagctttg
    17881 caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt ccatatcact
    17941 gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat catttttatg
    18001 tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat tgagtacttg
    18061 cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat gctgtcgatt
    18121 gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa cgctcagcgc
    18181 gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc agtcattcct
    18241 ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc gactgctatg
    18301 gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt gcattggcat
    18361 aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact gatcttttta
    18421 agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg tttgcaagaa
    18481 aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa ggtcacaggc
    18541 ctctttcttt tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc attattccag
    18601 ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa tcattgcgta
    18661 cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg ttcaagaaga
    18721 agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct ttggttcggg
    18781 aaggacagat gccggagtac atgcgatcgg gcaagtaatc cattttgatt atccagagga
    18841 gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg atattgctgt
    18901 aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca aagaaaagac
    18961 gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc attatgcaag
    19021 ttattttcct tacccacttg atttggaaaa aattcagcgg gcgcttcctg atttattagg
    19081 gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata aaatacgtac
    19141 gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt ttacatttag
    19201 gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt tgaaaattgg
    19261 caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag atcgtaatgc
    19321 agcaggacct acagcacacc ctgaaggact ttatttgtat gaagtgaaat atgaataaga
    19381 ggttatgata gaagtggtca gcttcaagaa ataagaaggt atttacgaaa attacttctc
    19441 aaaattttgc aaaatttcgg cttattttcg aagaagctac ttctggaaca ccgtttaaga
    19501 aagaggtcgt gagcctgtca tttaactctg agtcacaaag agcaatttca aaaaacagct
    19561 tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga atcatctttt
    19621 tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt gcccttggtg
    19681 cggacagtct gaacaggttc catcgtttct ttattttcta cgtcttcttc tgtgtgtgtt
    19741 tcgtggttgt tcttctattg ctggatcaat cgtgccataa aaggggcgat tgcggtcaag
    19801 gtaccagaca taaagaataa acaatgcaat acataggaca aaaaggattg cacctatcgt
    19861 aaatccttca ggcaagtaac tgaaagtgac agtatgttta cctgctggaa cttttatcat
    19921 caagaatgcg tctttgaaat tgctgacctt gactggtttc ccatcaattt tagctttcca
    19981 tcctttgtca taaggaatag ttgtcaccaa catggaatct tcagcggtat ttacagtccc
    20041 agaagctttt ctgccagtgg cttccatttc gacacctttt tctttgatct gattcattgc
    20101 ggcttgatag gctacaacat ctagaccaac gactttaggc tccatgaaac ttatttcttt
    20161 cgtcccataa aaacttgcag taaatgtcac tgttgtctct tgaggataat agccaagatc
    20221 atagtattga ccagaaatgt tgatttgcgt ttttcgcgat gtaccattga cagtgatcgt
    20281 cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac aaacttaagt aagcttgtgt
    20341 atttgcaggc acatggactt cccaagtgag ctcttttggc tggttatctg ctgtttctga
    20401 gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc aactgattct tcaaagtagg
    20461 tgtatagaat gtaaaatact gttcatttag accagacaat gtattgatca agtttgtctg
    20521 actggtcaaa ttatctgtca ctggctgctc aacatcatta acggacaacg gtgcagtaaa
    20581 tgctagtggc aatgcattga cattctgata cagtgtatag tcgccgcttt gaccagcttg
    20641 gcggaagcca tatttactga acgtgctttc gtttttagaa atgttatatt tgattcctgt
    20701 aaacccatcc atcagcaaag tattattgtt ataacggata ttcaaatttg tccctcttga
    20761 acggaaacct aatttatcca ggtaagagga agaattacga tttcttatag aagaaaacaa
    20821 actgatacca ctgtaaccat agttgatcga atcgtttgca gatactggat ccagattttc
    20881 caagcggtaa aacgagtcgg aatctttttt tgtttcattt acaagagctt tcaagtcagg
    20941 ataaggatca gtatataaac tgcgggaagc ataattccaa tcatcaagaa tgccatggat
    21001 catactgttc gtattgacca gtgcttcagc agacatgatc aataataaca atatactcag
    21061 atgattgaat gaaagttttt tatgctgata aaatcccaca gcaaaagcat aaatccctaa
    21121 gaaaaccact gtcaatacaa aggaagtcat cgtcacatag gtgtagcttc cttcaggttt
    21181 gatgccccat gccaccgcaa agagagcagc tacgaccaaa atgattccta acaacagccc
    21241 accatcgtct tgttctatct tttcccatcc ttttcctgca agcaggagca ctaagaaaga
    21301 gaacagatag gcataacgga ataaaaacat attcggtgca tgcataccat gccagaacaa
    21361 attcaatgga acaagataaa aacttgcgat aagtatggcg aaaagactgc cgtacaaaaa
    21421 tttatttttc aacggtactt tccgactggc aaaataaaac agacagagga ctaatggtag
    21481 cagacctgcg taaacgaacg gaatcgatcc gtattttgtc gtgtcataaa caccaatcat
    21541 atttttcatg attagatcca gataagaagt cgcttctgtt ttcaacgtag tgatctgact
    21601 cagtgcctcg ccatttgacc gaagatccag cactgctggc aatacaatga tcattgaagc
    21661 gccgcctgcc agtaaagaag tgataccata aggaacaatc gtttttttat attctgacca
    21721 gtttgtcaat agacgggcga taaaatataa gaaagaaaat acgccaatca taaaacccat
    21781 ataaaaactg gataaaaaca agagtaaata actgataaat aaaagtttcg gccgtttaag
    21841 atccataaca cgatgaatcc ccaaaatcac taatggaaga taaacaaatg catccagcca
    21901 catgatgatc tccgaatgtg cagtgataaa tgagatcaat gcgtaactga cgctaagtgc
    21961 aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga aaccaaaaag ccaatccggc
    22021 acagccgacc ttaagcaaag tcagaacata cagtgcatct ggcatcatct ggtttggaaa
    22081 aaagaacacc agtggcgtga agatcccgcc tagatagtaa gaaattaaag ataaataatt
    22141 caatcctaga gaagcattcc atgtataaaa aatactttgc tttccaagaa gaacatttcg
    22201 gaagctggca tgaaaattag aaaactgtga aaaggcatca cttgccaaaa tacttctgct
    22261 gcttccagga tagattccga tacttagata aacaagtgct aaaatcaaaa acggaatcag
    22321 gaaactagct gctgcgtagc gtccgttttc tctaaagaat gactttaaat tttttttcat
    22381 atttattttc caccaaattt ctatgaagtt gtattcagtt tatcatatcc ttgtttttct
    22441 ctgcttaaaa
//
LOCUS       DAHEYX010000023        22244 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHEYX010000023 DAHEYX010000000
VERSION     DAHEYX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.023"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            23..751
                     /locus_tag="MB219_000619"
     CDS             23..751
                     /locus_tag="MB219_000619"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCC8426355.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            751..1005
                     /gene="purS"
                     /locus_tag="MB219_000620"
     CDS             751..1005
                     /gene="purS"
                     /locus_tag="MB219_000620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCC8426356.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1007..1678
                     /gene="purQ"
                     /locus_tag="MB219_000621"
     CDS             1007..1678
                     /gene="purQ"
                     /locus_tag="MB219_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCC8426357.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1679..3901
                     /gene="purL"
                     /locus_tag="MB219_000622"
     CDS             1679..3901
                     /gene="purL"
                     /locus_tag="MB219_000622"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCC8426358.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3886..5325
                     /locus_tag="MB219_000623"
     CDS             3886..5325
                     /locus_tag="MB219_000623"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCC8426359.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5348..6400
                     /locus_tag="MB219_000624"
     CDS             5348..6400
                     /locus_tag="MB219_000624"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCC8426360.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6397..6975
                     /gene="purN"
                     /locus_tag="MB219_000625"
     CDS             6397..6975
                     /gene="purN"
                     /locus_tag="MB219_000625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCC8426361.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6972..8507
                     /gene="purH"
                     /locus_tag="MB219_000626"
     CDS             6972..8507
                     /gene="purH"
                     /locus_tag="MB219_000626"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCC8426362.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8523..9797
                     /gene="purD"
                     /locus_tag="MB219_000627"
     CDS             8523..9797
                     /gene="purD"
                     /locus_tag="MB219_000627"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCC8426363.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9919..10833
                     /locus_tag="MB219_000628"
     CDS             9919..10833
                     /locus_tag="MB219_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCC8426364.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10966..12531)
                     /locus_tag="MB219_000629"
     CDS             complement(10966..12531)
                     /locus_tag="MB219_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCC8426365.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12524..13975)
                     /locus_tag="MB219_000630"
     CDS             complement(12524..13975)
                     /locus_tag="MB219_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCC8426366.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13989..15005)
                     /locus_tag="MB219_000631"
     CDS             complement(13989..15005)
                     /locus_tag="MB219_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8426367.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15225..16313
                     /locus_tag="MB219_000632"
     CDS             15225..16313
                     /locus_tag="MB219_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCC8426368.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16367..17893
                     /locus_tag="MB219_000633"
     CDS             16367..17893
                     /locus_tag="MB219_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCC8426369.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17895..19100
                     /locus_tag="MB219_000634"
     CDS             17895..19100
                     /locus_tag="MB219_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8426370.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19357..20334
                     /gene="guaC"
                     /locus_tag="MB219_000635"
     CDS             19357..20334
                     /gene="guaC"
                     /locus_tag="MB219_000635"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCC8426371.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20598..22022
                     /locus_tag="MB219_000636"
     CDS             20598..22022
                     /locus_tag="MB219_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCC8426372.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         7184 a         3745 c         5064 g         6251 t
ORIGIN      
        1 taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag gaaaagccaa
       61 aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag atcaagcaac
      121 tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga ataatcaaat
      181 cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt ttattgaaca
      241 attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg aagtggttat
      301 ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag gtaaagaatt
      361 catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc catttatcaa
      421 tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg agctgaaagc
      481 ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg gtttggtttt
      541 agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg cggatgaagt
      601 aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg acaaagacgt
      661 ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata accgtctatc
      721 ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata tgtaacctat
      781 aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca tcgtatggga
      841 tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact tgctgatgca
      901 gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc aaatgtaaat
      961 atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga aatttgcggt
     1021 catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc gcgagatcat
     1081 gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat ttgatggggt
     1141 tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga ttgctcggtt
     1201 ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag tattcggtac
     1261 atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac tgagacgaaa
     1321 tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca agacggcctt
     1381 tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg gggaaggaaa
     1441 ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga ttgcctttac
     1501 ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga acaaagcagg
     1561 gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc taggatcaga
     1621 agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga ccgtatagat
     1681 gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct atgccgaatg
     1741 gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa gaatgcctaa
     1801 ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct ataaaaattc
     1861 taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag gccctggaga
     1921 aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag cagaaagtca
     1981 caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg gcggaatcat
     2041 cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat tgaggttcgg
     2101 cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag gtatcggcgg
     2161 atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg atccttgtta
     2221 tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca aacatatcca
     2281 gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa aaacaggtcg
     2341 cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag aagaacagca
     2401 acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg aagcgtgttt
     2461 ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg gagcagcagg
     2521 acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta tattgacatt
     2581 agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt tatcggaatc
     2641 acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc aagaactatt
     2701 tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg ggcaatatcg
     2761 gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag ctgaagatgc
     2821 accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc agcagttagc
     2881 tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc tgcagcaacc
     2941 gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac aaaccaatac
     3001 agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga ataaagcgtt
     3061 agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa tcggaggaca
     3121 aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc ccttggcgat
     3181 caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg aattatcgac
     3241 ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta tttcaggaaa
     3301 tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa tgattggtat
     3361 ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa aatcagggga
     3421 tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat tacaaaagat
     3481 gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg aaaagagaaa
     3541 ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc atgactgttc
     3601 agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc tagggatatc
     3661 tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa gatttgtgtt
     3721 gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg ctgctgtttt
     3781 agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg gtcagatcaa
     3841 aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc ttatgaagtg
     3901 aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga cgccgctagt
     3961 gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc aggtatcgtg
     4021 tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc tgaggtattc
     4081 aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca tgtacgttat
     4141 gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa atttcatgat
     4201 cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct tagaaaagag
     4261 ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt gatgcatttg
     4321 atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt gaatgaagtc
     4381 aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc tttggaccca
     4441 aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt gattgcctcg
     4501 gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca gcctggtgag
     4561 atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga cacgcagctt
     4621 tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat cgcagggatc
     4681 aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc gccagtaaaa
     4741 gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg ttatgcggaa
     4801 gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc ccgaacattt
     4861 atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc tgctgtacga
     4921 ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg gggtacgaca
     4981 agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca tgtgaggatt
     5041 gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac gcgtaaagaa
     5101 ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc agattcttta
     5161 ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga tgctccgtat
     5221 tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata tgactatgaa
     5281 gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca gaccaagaag
     5341 ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg tcgaagctgg
     5401 atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt taggtatgat
     5461 gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat taaaagagcc
     5521 agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa tcgaagcaga
     5581 caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata tcttagcaca
     5641 aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg atccgttacg
     5701 tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata tggcgctgat
     5761 tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg atctagccgg
     5821 atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga tcaacccagg
     5881 agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact ctcttgttcg
     5941 caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag aattgaaaaa
     6001 aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct tgcttccgct
     6061 gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt ttgtagaaaa
     6121 tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct catggccgat
     6181 tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg agatgtatga
     6241 aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag taaaagaaat
     6301 agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag tgatcgccaa
     6361 agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt ttttgcttct
     6421 ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg attggaatct
     6481 tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg tgcaactgcg
     6541 ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa agagtatgaa
     6601 gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt agctggatat
     6661 atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat caatatccat
     6721 ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt tgaagctgga
     6781 gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac tggaccaatc
     6841 atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt agaagaaaaa
     6901 atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat tgaaaatgga
     6961 ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg attgtagcat
     7021 tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga acaaagcatg
     7081 cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg tttcctgaaa
     7141 tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt ttaggaagac
     7201 gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca atcgactttg
     7261 tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc gaaatagcag
     7321 aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca gcaaaaaacc
     7381 atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt tcagaattac
     7441 gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa gtctttcgtc
     7501 atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg gaagaaaaag
     7561 aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac ggtgaaaata
     7621 gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg atcgcagcag
     7681 caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca gatgctgcgc
     7741 tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac atgaatcctt
     7801 gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat gaagcagatc
     7861 ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg ccaacagcag
     7921 aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca caagaagctt
     7981 tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt gcgcaggaag
     8041 aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta caagatcaag
     8101 atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag ccgactccag
     8161 cagaagcaaa agcattagcg tttgcttgga gagctgtcaa acatgtgaaa tctaatgcga
     8221 tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg aatcgagtcg
     8281 gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca gttttagcta
     8341 gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat ggtataaaag
     8401 caatcattca gccaggagga agcatcaaag accaagattc tattgatatg gcgaataaat
     8461 acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga ggagtcggat
     8521 aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt aaaaaacttt
     8581 tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg atgacaaaag
     8641 atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc cattttgtta
     8701 agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta aatgggatcg
     8761 tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca gcagctctga
     8821 tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc ccgacagcta
     8881 aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa aatggtgcgc
     8941 caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg gctaccacag
     9001 tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt ggggatagta
     9061 gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt ttggcgtttg
     9121 tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg gcatttgata
     9181 acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg caaatttcaa
     9241 aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg ggaatgaaag
     9301 aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg aaggaaggac
     9361 caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc gttctttctc
     9421 ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt acagtagagt
     9481 taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc ggttaccccg
     9541 aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt tctgtttact
     9601 atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt ctttatttag
     9661 tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc gcgttaatgg
     9721 ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct gccaatagcg
     9781 aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa acataatcgg
     9841 aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg ttgtgtatat
     9901 aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca gaaagcagct
     9961 gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa agtgattgag
    10021 acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg aaacggtgaa
    10081 tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga agatcttttt
    10141 attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa aaaacatatc
    10201 attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga tgctttactt
    10261 cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt tgatgaattg
    10321 gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat gcaaatcgaa
    10381 ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt tggcttgaaa
    10441 catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc aagtatcgat
    10501 catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca agcatggtca
    10561 ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt ccctgaatta
    10621 aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg cttggcttct
    10681 gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat gaacgtaggg
    10741 cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga ttggtacgaa
    10801 ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg tcaaaagggc
    10861 tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat attctgtctt
    10921 tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt tatttttgtt
    10981 tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt tcttaaataa
    11041 ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg aaatacgata
    11101 gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca gataatcgat
    11161 gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac ttaatgcaac
    11221 ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt tgataaaatc
    11281 caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg gcagagcagt
    11341 taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg cgctgattgc
    11401 taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct tttctttcag
    11461 gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg ttcgagttgg
    11521 tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt tcgataatga
    11581 agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt tcgcttcttg
    11641 aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt caagtatcat
    11701 ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag gaccaatggc
    11761 gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt gggctacctg
    11821 ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt tgatgaatcg
    11881 agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact tcaaataatc
    11941 actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta ataatacggt
    12001 catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg tttcactgat
    12061 cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt cgaaaacaag
    12121 atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg ctttttctat
    12181 tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg cctgtacctg
    12241 gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga gacgagtcca
    12301 aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag gcatttggat
    12361 atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca tctcaccatc
    12421 tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta tttgttcccg
    12481 caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca tgtttgtttc
    12541 ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct tctcggtatt
    12601 tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga cgcttgttta
    12661 cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct aactctttac
    12721 gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact ttgaattgga
    12781 tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg acgtacttcg
    12841 ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa atagaaacag
    12901 gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat cttattttta
    12961 agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc cagtcttgtc
    13021 ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt
    13081 caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa ataaaatggg
    13141 gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat tgtttcaaag
    13201 cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta gcggtaagtg
    13261 tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct gaagaaagtt
    13321 gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct aaaaagcgac
    13381 gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact attgttaata
    13441 gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa
    13501 caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga ttgatgatct
    13561 cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg ttttgataat
    13621 cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct ttaagtgtat
    13681 ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata ttttcctcat
    13741 tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc aaaacttttt
    13801 gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc tgttgataaa
    13861 tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca gcaaatagaa
    13921 gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt gtcatgatac
    13981 ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga aaagacagtc
    14041 tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc aaaataacga
    14101 gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac acaattgacg
    14161 attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac aatgatcaaa
    14221 tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg agtcatttca
    14281 tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa atatcgtttc
    14341 atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc
    14401 ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt gattgattcc
    14461 aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc aagttctagg
    14521 attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa acccgcccga
    14581 tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg atcattcgtt
    14641 tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc ttttaataca
    14701 gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt ataggcaatg
    14761 aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg gtagagaact
    14821 tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt ccgccagcta
    14881 ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc ctttgtatta
    14941 ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt
    15001 ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata aaagggaaaa
    15061 tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa
    15121 aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt aacgctttct
    15181 ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa aacaaattta
    15241 gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt gcgtgtggca
    15301 attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg accaaatcag
    15361 cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa ctgggttata
    15421 aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa atagaaaata
    15481 tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg gctttaactg
    15541 atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat cgtttactga
    15601 tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta ggtgtatcgc
    15661 aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca ttcacgattg
    15721 aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac ggagtgatgt
    15781 ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga cagacgagtt
    15841 tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg atggataatc
    15901 tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca tatgatccaa
    15961 taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa agtaaacctt
    16021 tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc attgccgggg
    16081 aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg attgaaatga
    16141 tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact tatgataatg
    16201 gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa gataattatc
    16261 aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat taagaggaac
    16321 caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg cggattgtat
    16381 tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag attgcgtgaa
    16441 tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag cgggaaaatc
    16501 aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg gggaaatctt
    16561 ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa aaggcatcgt
    16621 catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga atatttttct
    16681 cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa agaaaacata
    16741 tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat cccagattgg
    16801 cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg tccgtctgtt
    16861 gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt tgctgagatt
    16921 gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata agctgaatga
    16981 aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga ttgagacact
    17041 agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca gagatttagt
    17101 tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag tagaagactg
    17161 gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca acttgcattt
    17221 gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa ctgaattcgc
    17281 tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat taaaggaagg
    17341 ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg cttatgtctc
    17401 ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa acgtgacgct
    17461 gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag aagtaactga
    17521 ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc aaattgtcag
    17581 tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga tgacacaacc
    17641 cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa aatatgagat
    17701 ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta tctcttctga
    17761 acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag gacgtattac
    17821 aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa tgacgaaaga
    17881 agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag aaaaaactaa
    17941 atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc attttagcgg
    18001 gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca ttgaatataa
    18061 ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg ttattagttg
    18121 tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt ggtgcaattg
    18181 cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc gcatgtcttg
    18241 cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca gggataccgg
    18301 cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa gttgttctag
    18361 gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg ggttatttac
    18421 cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga atgatcatca
    18481 cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat catttatttg
    18541 aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc gttgtactag
    18601 gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc attcttggtt
    18661 tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga cagatttatg
    18721 cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag cgaatcacgt
    18781 tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt ttagctgctc
    18841 gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca atggcggcag
    18901 tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt gcgattgtcg
    18961 gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc ggggtagact
    19021 ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac ttgtataata
    19081 agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg tgacaagaca
    19141 tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg tatagactaa
    19201 aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg atttttttgc
    19261 ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta ataatgttcg
    19321 tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga ttacgaagat
    19381 attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga tacaacggtc
    19441 acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca gacgattatt
    19501 gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat gcatcggttt
    19561 gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt gattacctcg
    19621 atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc agaagaatcg
    19681 ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc agttattgat
    19741 atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg taatgttggt
    19801 acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa agtcgggatt
    19861 ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg aggctggcaa
    19921 ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc tgacggtggg
    19981 atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat ggtcatgatt
    20041 ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga aaatggaatc
    20101 gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa acgaaatgtt
    20161 gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact agtggaaatg
    20221 caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga agccattcga
    20281 aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat ctaaaaaata
    20341 aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag aacagccaaa
    20401 caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat acaagctttt
    20461 gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc ccttatgttt
    20521 tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa aaaatagaca
    20581 atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg atttatcgtc
    20641 aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct ggaagtcaac
    20701 ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc gttgtccatg
    20761 cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc ggatggtttg
    20821 ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg caaatgatct
    20881 ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca aagcagatcc
    20941 aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt ttgccgaatt
    21001 ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa atggatcttt
    21061 taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa tttgaattgg
    21121 cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc tttttgattt
    21181 tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct tttttttacc
    21241 gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg gatgaagaag
    21301 ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa gcagtgctta
    21361 tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga cgagaaaagc
    21421 tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta tttacggatc
    21481 tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg gataatgtgt
    21541 tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt tgggtgatag
    21601 gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag ttttcgatga
    21661 atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat ttgaaaatgc
    21721 aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg ggagattcac
    21781 aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt acttgggatg
    21841 ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt ggaattgcac
    21901 caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt aggctggatg
    21961 aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg ttaacagaat
    22021 aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt tcaagctgtt
    22081 tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca ttatttgtac
    22141 gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata acaccaacgt
    22201 ttttaaatag aaacagatgg ttgtaagttt gattagaaaa taga
//
LOCUS       DAHEYX010000024        21976 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHEYX010000024 DAHEYX010000000
VERSION     DAHEYX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.024"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            23..3301
                     /locus_tag="MB219_000637"
     CDS             23..3301
                     /locus_tag="MB219_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCC8426373.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3842..5749)
                     /locus_tag="MB219_000638"
     CDS             complement(3842..5749)
                     /locus_tag="MB219_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCC8426374.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5879..6754
                     /locus_tag="MB219_000639"
     CDS             5879..6754
                     /locus_tag="MB219_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCC8426375.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6834..7808
                     /locus_tag="MB219_000640"
     CDS             6834..7808
                     /locus_tag="MB219_000640"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCC8426376.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9133
                     /locus_tag="MB219_000641"
     CDS             7988..9133
                     /locus_tag="MB219_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCC8426377.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9270..9614
                     /locus_tag="MB219_000642"
     CDS             9270..9614
                     /locus_tag="MB219_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCC8426378.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9885..11171
                     /gene="efmA"
                     /locus_tag="MB219_000643"
     CDS             9885..11171
                     /gene="efmA"
                     /locus_tag="MB219_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCC8426379.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11309..11908)
                     /locus_tag="MB219_000644"
     CDS             complement(11309..11908)
                     /locus_tag="MB219_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCC8426380.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12312..13433
                     /gene="mnmA"
                     /locus_tag="MB219_000645"
     CDS             12312..13433
                     /gene="mnmA"
                     /locus_tag="MB219_000645"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCC8426381.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13679..15100
                     /gene="arcD"
                     /locus_tag="MB219_000646"
     CDS             13679..15100
                     /gene="arcD"
                     /locus_tag="MB219_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCC8426382.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15114..16232
                     /locus_tag="MB219_000647"
     CDS             15114..16232
                     /locus_tag="MB219_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCC8426383.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16292..17584)
                     /locus_tag="MB219_000648"
                     /pseudo
     CDS             complement(16292..17584)
                     /locus_tag="MB219_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            17716..18294
                     /locus_tag="MB219_000649"
     CDS             17716..18294
                     /locus_tag="MB219_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8426384.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18423..18800
                     /locus_tag="MB219_000650"
     CDS             18423..18800
                     /locus_tag="MB219_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCC8426385.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18733..20805
                     /locus_tag="MB219_000651"
     CDS             18733..20805
                     /locus_tag="MB219_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCC8426386.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21016..21963)
                     /locus_tag="MB219_000652"
     CDS             complement(21016..21963)
                     /locus_tag="MB219_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCC8426387.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         7103 a         4029 c         4674 g         6170 t
ORIGIN      
        1 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
       61 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      121 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      181 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      241 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      301 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      361 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      421 gacctattat tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga
      481 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      541 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      601 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      661 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      721 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      781 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
      841 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
      901 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
      961 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1021 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1081 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1141 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1201 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1261 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1321 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1381 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1441 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1501 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1561 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1621 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1681 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1741 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1801 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     1861 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     1921 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     1981 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2041 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2101 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2161 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2221 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2281 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2341 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2401 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2461 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2521 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2581 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2641 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2701 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2761 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     2821 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     2881 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     2941 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3001 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3061 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3121 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3181 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3241 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3301 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3361 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3421 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3541 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3601 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3661 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3721 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3781 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3841 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     3901 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     3961 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4021 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4081 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4141 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4201 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4261 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4321 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4381 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4441 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4501 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4561 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4621 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4681 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4741 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4801 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4861 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     4921 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     4981 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5041 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5101 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5161 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5221 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5281 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5341 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5401 gtaatgtatc caacagccat tctggaaatt gcttattcaa ttcgtcgaaa gactgcaatg
     5461 attcaacttt ttcgattata ggttgaagcg gttgagcatg caactcattt cttcggtcat
     5521 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5581 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5641 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5701 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5761 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5821 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     5881 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     5941 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6001 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6061 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6121 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6181 tcccgttttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt
     6241 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6301 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg ggtggatgct
     6361 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6421 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6481 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6541 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6601 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6661 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6721 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6781 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6841 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     6901 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     6961 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7021 aatcaacgaa tgcacctgtc aatgattact atatggaact actgatcatg atcgatgcaa
     7081 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7141 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7201 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7261 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7321 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7381 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7441 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7501 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7561 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7621 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7681 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggtgaagcgg
     7741 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7801 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaga agtgagcagc ttcaaggagc
     7861 tgcgacaaga cttttgtcac agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     7921 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     7981 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8041 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8101 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8161 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8221 taatacagct atcttagaaa cagcgttctc gcgtcaaaaa gaaggcagac atctaatcac
     8281 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8341 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8401 agcgttacga gaagatacga tcttggtctc tgtgatgtac gggaacaatg aaatcggaaa
     8461 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8521 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8581 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8641 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8701 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8761 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8821 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     8881 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     8941 taatggggtc gctatttcta ctggttcagc ttgtacagca ggaaatatcg agccttcaca
     9001 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9061 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgatcaat tagttcgtgc
     9121 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9181 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9241 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9301 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9361 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9421 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9481 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9541 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9601 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9661 gatgactaac tttgtaagga cgacgcaaat tagttgtctt ttttattttt aaatttaaca
     9721 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9781 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9841 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
     9901 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
     9961 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10021 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10081 gcccatttgt aggaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10141 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10201 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10261 tgccaacgat ccagtcgatt atgccgacaa tcgttccatc ttctcacata acaaggacga
    10321 atggacagtt aggaatggtc caatcagcga atttcatcat tgcgccagct ctaggtgcgg
    10381 ctttgttttc agtcgttcct gtcaattacc ttattttatt agatgtattg ggggcagttt
    10441 ttggtgtcgg cttattgatt tttgtgaaga tcccaaaagt ttctcctgaa atattggaag
    10501 ttccgcttac tatttttaaa gatgcaaaat ttgggctgca gcaattgatg gataataaag
    10561 ggttatggta cataacgatc aatggtgcat ttgtgatgct gttgttcatg ccagcaatca
    10621 gtctgtatcc attgatgacg ctagattact ttggtggttc cgttggacag gcgggagcag
    10681 tggaagtagt ctacgcagtc ggaatgctct taggcggtgc actgatcagt tttataggaa
    10741 catggaaaga ccgaatgaaa cctattatta tagcctacat cattatgggc ctgacgatcg
    10801 gtgcaagcgg attggttcca aacgatagtc aagggttctt gtactttctt atcttgaatg
    10861 caggagcagg ttgtgcaacg ccatatttca atacactgct gatggcaatg atccagcaga
    10921 gttatgaatc gaatgtttta ggtcgtgtct tagggaactt caactctttg atgaacctgg
    10981 caggtccaat tggattatta tttgcaggac cattagctga tcgtttaggg gtagaaaaaa
    11041 tgttcttgtt ttccggaatc gggattttat tatgcggaat cgttttattt ttaacgtcag
    11101 ctgcccgaaa ttatgataaa gaattacaga aaaaactggt aaaagaacac catgagcaaa
    11161 aagacgaata gtatggaatg gagttgtgac agaagtcttg ttacaactcc atttctgatc
    11221 ctaatgttcc tgtggcaata agcctactca aatgaaaaat atgagtaatt atctttattt
    11281 gtttaatact tgatataggg cagttttttc acgacctatc gtacttcctc tttgcttcat
    11341 agatttcttt gatttgttgc tcagataacg gagtgccaaa tggtagtttt tcctttaaga
    11401 gttctctttc cggtgcgtct acgcctacat tgacatcgtt ctcgataggt acgctgtaat
    11461 gatggatatg tccatgaaga ttgcggatag tgtcgttagg cccgagcaac atcggataat
    11521 gagtcaagta atattgttga tggtcgaatt taacgatggc acctacatca taaaaataaa
    11581 atttgggact tccgtcagta agcctaggat catttttttc taaataacgg aaaaaagcgc
    11641 gactgtcatg gttccctttg atcaagtgga ttgttccttg cagttgagtg accagttcca
    11701 aaatttctgc atttgctttt tcatattgcg gatgcatagc aaggtcgcct agatgataaa
    11761 catggtcggt ttcttttatt actgcattcc agctagtgat caactgttgg ttcatttcat
    11821 ccacatttgc aaagagccgc ggagcaaagt cgttttttcc taataagcgt tcatggaaaa
    11881 agtgcatatc actgataaag taattcatgt atcttttgcc tccctttttt cttagagtaa
    11941 acaaaatcac ttatctgagc aactcataac atctaaaaag aaaaggttgt aaatgagacg
    12001 tgcagcttta agcgataaga agcaattttg aaaaatagtt tttcacaatt tataaaatta
    12061 ttccttattg ccgaaaggct ggcccttttt atgagtatgc caatttttta tatcgaatgc
    12121 tcaaaagatg agtgattttt tggttgacta tcatgtttgt gtactgaata ggtccagtag
    12181 aacagtattt ttacgacttg ataattaagg gatgaaattt tcagaaatat gacgtataat
    12241 gtaaaatact gaaaagttgc gaccttcaac tcgtagatgt ttcagaatct aattgaaatg
    12301 atggtgatat catgacagac aacagcaaaa cacgcgttgt cgtagggatg agcggcggtg
    12361 tcgactcatc tgtaacagca cttttgttaa aggaacaagg atacgacgtg atcggaatat
    12421 tcatgaaaaa ttgggatgac acggacgaaa acggtgtatg cacggccacg gaagattata
    12481 aggatgtagc gaaagtagct gctcaaatcg ggattcctta ttactctgtg aattttgaaa
    12541 aagaatattg ggatcgtgta tttgaatatt tcctagccga ataccgcgca ggacgtacac
    12601 ctaacccaga cgtgatgtgc aacaaagaaa tcaaattcaa agcattcttg gattatgcaa
    12661 tggatcttgg cgccgattat gtagctactg gacattatgc gcaagtcact cgcgatgaaa
    12721 acggcgtggt ccacatgctt cgtggagtag acaataataa agatcaaact tacttcctta
    12781 gccaattatc acaagaacag ctttcaaaaa cgatgttccc tctaggcggc atggaaaaat
    12841 cagaagtacg ggcaattgct gaacgcgcag gccttgcgac tgctaagaaa aaagattcaa
    12901 cgggaatctg ctttattggt gaaaagaact tcaaacaatt tttgagcaac tatctgccag
    12961 caaaaaaagg aaatatggtc actcttgatg gtgaagtcaa aggacaacat gctggcttga
    13021 tgtattatac gattggtcaa cgtcaaggac tagggatcgg tggcggagga gattcgcaag
    13081 aaccctggtt tgttgtagga aaagatctag cgacaaacac attatatgtg gggcaagggt
    13141 tccatcatcc tgcattgtat gccacaagct tggatgcaag tgaaatccac tttacgacga
    13201 atgaaccaat gccaaaagaa ttcaagtgta cagcgaaatt ccgttatcgt cagcaagacg
    13261 ttcctgtgac agttcgctta ttggatgata atcgtgcaga agtcgtattc gacgaacctg
    13321 tacgtgcaat cactcctgga caagcagttg tattttatga tggaatggaa tgtctgggtg
    13381 gtggtttgat cgaccacgca tatcaagaaa caaaagtttt acaatacgtt taagcgatat
    13441 taaatgaatc aaaacagcta tggctaacaa aagaaacacc ttttcttttg gatagcatag
    13501 ctgttttttt gcaataaaat taatttttat tcattttata cttgaaatac ttgtgtaaaa
    13561 agttcataat atccaataat atgaataaag ttgctttttg ttaagcaaga gcagttgcaa
    13621 aatagaaggg aatcttttgc aagtgatttg ttcaatttta taagtgaggg tgatggaaat
    13681 ggaaaagaaa tcgacgggta ttagcaaatg gggactggtt gctctggttg tcagttcgtc
    13741 aatcggaagc ggtgtgtttg ggatcacaag tgatctggct agttctgcag ctccagggcc
    13801 agcgatcctt tcatggataa tcgttgggat tggtatttta gctttggtat tatcgctgaa
    13861 tcatttagga gaaaagcgtc cagatctaga tggtggtatt ttcggttacg cggaagcttc
    13921 atttggtaaa ttaggtggct tcatcagtgg ttggggttac tggttgtctg cgtggctggg
    13981 aaatgttgct tttgcaacga tgctgatgag cgccatggga gaatttttcc cgatattcaa
    14041 aggtggacaa aatgttcctt ctattttatt tgcaagtatt tttatttggg gtctgacttt
    14101 attagtaaac aatggagttg aaggagcgag cgtcatcaat acaatcgtga cgatctgtaa
    14161 attagtgcca ttattcctat ttttaatatt tgtatttatc gcatttaaag tgaatatatt
    14221 cactgctgat ttttggggta acgtatctga taatctagtg aatggaacag ggcagcaagg
    14281 aacgatctgg ttgcagatta aaggttgttt gatggttatg atgtgggtct ttgtagggat
    14341 cgaaggcgca agtgttttag ccaaccgcgc gaagaaacgt tcagaagccc agcaagcatc
    14401 gatcattggt ctgctttgtt tattgtttat ctatatcctt gcttcgatct tgccatacgg
    14461 cgtcttatct caagaagagt tagcaactat ttcacaacct gctatggcaa atattttaaa
    14521 aggaatcgtg ggaaattggg gagcagcgct gatcaatatc ggcttgatta tttcgatcat
    14581 tggttcttgg ttgtcttgga cgatgctccc agcagaaaca acgatgttga tggctcgaga
    14641 cggtctgttg ccgaaaggtt ggggaaaaac gaataaaaaa aatgccccaa cttattcgtt
    14701 agttgttact gcaatcttga ctaatctatt cttattgact ttcttagtta ctgattatgc
    14761 ctatcaattt gcctattctt tatgtacagc ggcaattttg atttgttatc tacttgtcgg
    14821 gatctatcaa gtccagtttt ctttaaaaga aggaatcaaa aaacaagtag tgatcggtgg
    14881 gattgctgta gtttttgaat tgatgggcat cattttagca ggattttctt atgtcctttt
    14941 atgtagtatt gcctatcttc ctggtttttg cttctactgg atcgcttgtc gtgaaaccaa
    15001 acatcaaatt acgggaaaag aaaaagtaat gattgggctg atttgtgtgg ccgcactgct
    15061 ttcaatcggg ttgcttgctg taggctggat caagatataa gggggaaaga aacatgaaaa
    15121 tcgcaaattt cggtgtagaa gaatggctaa ataaatggga aaagaaagcg gtatatgata
    15181 ttgcacaaag ctcgatcgaa gcattgacat tagacgaact cgttggctta gatgggacta
    15241 gcgtaagtaa atttttcgaa aaacgcaaaa acgaaccatt ggactatggc tggatcgaag
    15301 gatcagatgt tttcaaacaa gaagtcgctt ctctttatca aaccgtcgat ccggaaaaca
    15361 tcttacaaac gaatggagcg actggtgcta atcttttagc tctatacgcg ttagtggaag
    15421 caggagacca tgttgtttcg atgcttccta cttatcagca attatatgac atccccaaat
    15481 ctttaggtgc cgctgttgac tttgttcatt tgaaggaaga agaagattgg cagtttgatt
    15541 tagaacagct agaagcctta gtcaaacctg aaacaaaaat catctgtttg aatagtgcaa
    15601 acaatcctac aggcacattg ctagatcgga aaactttaga aaaaatcgca gaaattgctc
    15661 gtacagtaga tgcatatgta ttgatagatg aagtatatgc gcctttaacg gataaaggtg
    15721 aattcttgtc gatcgtagat gtctatgaaa aaggtatagc aaccaattcc ctatccaaaa
    15781 cctactccat tccaggtatt cgtattggct ggacagctac tggacctgag ttggcagaag
    15841 tttttcgaaa gtatcgagac tacacgatga tttgcggcgg agtcctgtca gatgatcttg
    15901 ctgtacatgc attgaagaat aaagaaaaaa tattggcgcg aaacaaaaag atcattacag
    15961 agaaccttgt gattttaaaa caatgggtag ccaatgaacc gaaagttgaa cttgtcgctc
    16021 ccaattacgt ctctacttct ttcattaaat tagccatcga agaagatgac cagacatttt
    16081 gtatcaattt attagaagaa acaggagtac tattagtacc aggaagtgcc tttgatttac
    16141 cgaagcatgc ccgactaggt tactgttgta aaaaagaaac cttggaaaag ggattattcc
    16201 tcttatctga gttcttgaaa aaacaggcct aaacaaaaac actgcaagga cagtctgtga
    16261 caactgttca agcagtgttt ttcatttacg cttattgtct atgagaagga gagtgccctg
    16321 aagctatgga gactcctgaa gctttttctt ctgcagtttc ttgctgatgg atctcttgtg
    16381 tattgtgtcg tacaagcaag aagttgacca aaacaagaag agaagtcgaa agaaagacgc
    16441 cgcgataacc aaatgcactg gaaacactgg agccgatcat aggccctact acgttaccag
    16501 ttgcttggaa agattggtta taactgaaga tccgtccagc agcttgatga ggcgaatatt
    16561 ttgtgatcaa tgcttggaca gcagggagca gacaagcatc agagatacca atcaaaaaac
    16621 gtaaggcagc taattgccaa acattcgtga cgaatgccat tgggatataa accagaatcg
    16681 caaaacccaa accaatcgca agtattcttt cactcccaat cttgtcacct aagcgaccaa
    16741 atcttggagc ggctatcaaa gtggcaatcc caggtatcga agcaatgatt ccgctgatca
    16801 acgtaacatt cccatgtcca tgcagcaatt ggcgaatgta cagactgatg attggactaa
    16861 tggaattgtt ggatgcttgg atgatcatcg ttgtgacgaa cataccgatg acgacgtgtg
    16921 gatatttcaa gtctttgaaa atctgtttgg cagaaaccat gttttctttt ttcactggga
    16981 caaatttttc atggacgaaa aacagactca acaagaagac gaaaaataag atgatccctg
    17041 taatgaaaaa ggtagggcga tagccaaacg ctgaagcaga gatcccgcca agaagcggtc
    17101 caagtaatgt tcctgtaaca gaaccagttg ctaaggttca aagaacttga ccactttttt
    17161 cttttggcgt cccagttgcg acaagggcag tggcattact gatataacca gaaaaaatcc
    17221 cttgaagcag acgcaatgcg actaattgat acacactagt cacgcaaccc atcaaagaaa
    17281 tgacgattgc cattcctaat gaagcacgca acagcatcaa ttttcttcct ttttggtcgg
    17341 ctaattttcc ccaccaagga gagataagcg tagtcaccaa gaaagtagat gaaaaagtca
    17401 atccgctcca aaagttcaat tgtgacgtac tataattacc taacgtatct atgtaaagtg
    17461 acataaaagg catgactaaa ctaaatccga taccagccat aaatgttcca aaccataaga
    17521 caaacaagtt ttgttcccaa gtttctcttt tcctaaataa tttttccttt attcttgcag
    17581 ccaattagac tccttctttc tgaaaatttt catctacagc tctttattat aaactacaat
    17641 atgtagttta tcaatgaaag tctctttttt ctctttcttt ttcgtggtaa actgaaagag
    17701 tgaaggattt attttatgag aggaaacatg gaaatgaaaa aaagaaatct gtctcaagag
    17761 aaaatcatcg actgctttcg agaactagca gaagagatgg atgttcagca aataaccttt
    17821 cagcatttag ccaaacgctt ggatatcaag gctccctcgc tgtataacca tttcaaaaat
    17881 attcgtgatg tcaaaacagc gctaacagcg aaacttctgc aggaacttaa tgaccagctg
    17941 cgacgggctc ttgtgggaaa aagtggtcag gaagcactgc agatctatgc agaaacttat
    18001 cgatcctttg ctttttcaaa tcaagcagtg tatgagttac tgatcagtgt accgcatacc
    18061 aatgaagctg ttttattaga tggaatccat gaaacgaatc agatcatttt gcagatattt
    18121 gaggcttttc aattaacgaa agaagaaaaa gttcatcgaa gcagggaact tcgtagtatg
    18181 atccacggtt atctttcctt acgtttttta ggatacttta caaaagaacc aacaatcgat
    18241 ccagaagaca gctatttttg gatgatcaat aactttatcg ctactttgcc ttaaacaatc
    18301 agattcattt gtaacaattt taagatgttc attaaatgac ccttttataa gagaaaattc
    18361 ttgcaacttt cttgaaaagt gactaagatg agtctagaaa agctgaaaga aggagcacga
    18421 gtatgtatca agtaattacc atgttcgggg acaatgagcc ttggtggttt tttgaagact
    18481 gggaagaaga tattgaagaa gaagaaacat tcgagtcttt cgaagaagca cgacaagcgt
    18541 acgaaaaaca atggaacgaa atccatcaaa attatgaata tatcaatgca aaaagtaact
    18601 ttctcagcgc cttttggaat gacggagatg agcgctggtg tgaagagtgt gacgatgatt
    18661 tacagcaata caagggactt gcgttgttga aaaatcatca gcccatcaca gtagaaagca
    18721 gaaaggaatt ttatgaaaca actaattcta gcggaaaaac caagcgttgc gaaagaccta
    18781 agcaaggtgc ttggtgctaa tcaaaagcac aaaaattatt atgaaggacc caaggtgatc
    18841 gttacttggg cccttggtca tttattagga ttgaaaatgc cggaagatct gaataaggaa
    18901 tggcagagct ggcaaatgga cacgttgcca atgatcccta aaaaactagg aatcaaacca
    18961 cttcctaaaa caggacatca attaaaagca atcaagcagc tggctcagcg aaaagatgtc
    19021 tcagaagcag tcattgcgac agatgccgga agagaagggg aacttgttgc tcgctggatc
    19081 ttagagtatg tgcatttcaa taaaccagtg aagcggttat ggatctcttc tcaaacagat
    19141 aaagcaatca aaactggatt caagcaacta agacctgcga aagaatatga tgcattgtat
    19201 gaatctgctc ttgcccgggc aaaagcggac tggctggtag gattgaatgt gaccagagcc
    19261 ttaactgtga agtacgcgga taatttatca gcaggccgtg tgcagacacc gacactagca
    19321 ttagtgagag accaagaaaa gaaaatcgag cagttccgtc cacaaacgta tttcacgatc
    19381 acgttgcaag ctggaaaaga acaagccaaa atgcttcaaa aaaatcaatt tgctttaaaa
    19441 acacaagaag aagcacaaaa acttgttcaa ttgatcagta caagcaaagg aactgtcaca
    19501 gaagtccaag aaaaattaaa aacggaatct gcgccattgc cttatgattt aacagagatc
    19561 caaagagaag ccaatcaacg ctatggctat tctgcaaaga aaacattaag tcttgtacaa
    19621 agtttgtatg agacccataa gatcgttact tatccgcgta cagacagcaa atatctgaca
    19681 aatgacatga aaagtacgat gaaagaacga ttgcaagcag tcagcgactt tgctcctgag
    19741 gtcaagcagt atttgaaaaa cggagccatc gtccaacaga caaaagtatt ccaagacagt
    19801 aaagtgaccg atcaccatgc acttttgcca actgaaaaca gagcacgtta tgaaaaatta
    19861 agtaatgaag agcgaaaaat ctatcaaatg attgtctctc gttttttagg actgtttgct
    19921 caaccccata aagtcagcca aacaaaagtt acggttgaat ttggtaaaga acgattcatt
    19981 tttcggcaaa atcgtgtcgt ccaagcagga tggaaaggcg aaactgaaac tgagacggaa
    20041 acagtcaaat gggaaaaagg aatgagaatc aatcccgatt tcgccatcaa aaaagaacta
    20101 tctgcaccgc ctaaaccatt gtcagaagca agcttgcttg gtcaaatgga aaaatacagt
    20161 ctgggaaccc cagctacgcg tgcagagatt atcgagaaac taatcaaatc tgaactgatg
    20221 gaacgtaccc ctcaaggact gcaagtatct cctaaaggta agcaattatt agtattggta
    20281 aatccctctt tagtaacacc agatttgaca gaaagctggg aaaaagacct tgaagctatt
    20341 gctgcaggaa agaaacaagc aggaaccttc ttgaatggaa tcgaaaaaga aacaaaacgc
    20401 ttggtgaatg agatcaaatc aagcaaacaa gaatatcaag atttctcgat tacccaaaag
    20461 aaatgcccag aatgtggggc caatctgcgc gagaaaaaca cacgtgacgg caaaatctat
    20521 gtctgcacca attcagattg caactaccgc agaagaaaag atccaaaagt ctctaatcat
    20581 cgatgcccgc aatgtcaccg gaaaatggaa atcatcgaag gtaaaaacgg tgcttatttc
    20641 cgttgtaaat acgatggaac gacagaaaaa ttgcttgata aaaaagagca gaagaagaaa
    20701 atgaccaaac atgaagaacg acgcttgctg aaaaaatatg cacaagcaga agaaccagaa
    20761 gaaagcccat tagcagcagc actaaaagca gcaatgaaga agtaataaga tatgaaagag
    20821 gttgtgatag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca
    20881 aatttttgtg aaatttcggc ttatttccga agaagctact tctggaacac catttattcg
    20941 gaaaaaagga ctgtgacaag acttttgtca cagtcctttt tcatttaaaa aaatgtttac
    21001 ttcatttgac gtatattatt ggtgaggctt caattcattt ggcttcactt tttctgtttc
    21061 tcttatgata taaacaggac gctgtttcgt ttctaagaag attttcccaa tatatttccc
    21121 gatgattcca agacataaca attggattcc accaatgaac aggaagatcg agaccatgga
    21181 aggccatcct gatgtaggat caccaaacaa caatgcccgc aaaataatga acaaaattgc
    21241 aataccagaa ccgacacaag acaaagcgcc gataaatgaa gcaatcgtta acggtgcatc
    21301 cgagaagttc acgataccat caatcgagta gttgaataat ttccagaagg accaagatgt
    21361 ttcgccagcc acacgttcgc ggttttcata agaaatatat tctgttttaa atccgaccca
    21421 gctaaataat ccttttgaaa atcggttgta ttctgttaat tctaggacgg catccaccat
    21481 ttgacgagtc atcaagcgga aatcacgagc gccatcgacc atttctgtat cgctgatttt
    21541 attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt atcgcaggct cgccttcacg
    21601 agtcgaacgc cgggtgccga cacaatcgat atcagaagta gtgatccggt tgatcatctc
    21661 aggaagcagt tcgggcggat cttgcagatc aacatccatc acggtcacta aatctcctgt
    21721 agaagcttgc aatccagcgt acagagccgc ttctttccca aaatttctgg aaaatgagac
    21781 aaagcggaca taaggataag ctgaggctag cttcttgatt gctgctaacg taccgtcttt
    21841 tgatccgtcg tcaataaaca aatattcgaa ctcatgggca cctttgaatt tttcaacctc
    21901 atcaaaaaaa agaggaacag ttttttcttc attaaaacat gggacaacaa ttgatacttt
    21961 catcgtttct atcctt
//
LOCUS       DAHEYX010000025        21903 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHEYX010000025 DAHEYX010000000
VERSION     DAHEYX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.025"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1415
                     /locus_tag="MB219_000653"
     CDS             <1..1415
                     /locus_tag="MB219_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8426388.1"
                     /translation="LLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMV
                     IAAALVYPTIVSAYSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLH
                     ESIRNLLTPFICIIVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFW
                     QVLVIFGLHWGLVPVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSL
                     SLSAFITALFGITEPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSIL
                     SFPIYIGEGFVWLVASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDL
                     SEVNDPTFASGSLGEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHI
                     GIDTVNLNGKYFQSAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNI
                     ITSHKKNNQTRKLASLGGSI"
     gene            1412..2869
                     /locus_tag="MB219_000654"
     CDS             1412..2869
                     /locus_tag="MB219_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCC8426389.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            3453..3887
                     /locus_tag="MB219_000655"
     CDS             3453..3887
                     /locus_tag="MB219_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCC8426390.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            3988..5079
                     /locus_tag="MB219_000656"
     CDS             3988..5079
                     /locus_tag="MB219_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCC8426391.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            5109..5786
                     /locus_tag="MB219_000657"
     CDS             5109..5786
                     /locus_tag="MB219_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCC8426392.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            6118..7413
                     /locus_tag="MB219_000658"
     CDS             6118..7413
                     /locus_tag="MB219_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCC8426393.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            7403..8863
                     /locus_tag="MB219_000659"
     CDS             7403..8863
                     /locus_tag="MB219_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426394.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            8999..10495
                     /gene="glpK"
                     /locus_tag="MB219_000660"
     CDS             8999..10495
                     /gene="glpK"
                     /locus_tag="MB219_000660"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCC8426395.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            10509..12332
                     /gene="glpO"
                     /locus_tag="MB219_000661"
     CDS             10509..12332
                     /gene="glpO"
                     /locus_tag="MB219_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCC8426396.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            12333..13049
                     /locus_tag="MB219_000662"
     CDS             12333..13049
                     /locus_tag="MB219_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCC8426397.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            13355..15283
                     /locus_tag="MB219_000663"
     CDS             13355..15283
                     /locus_tag="MB219_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCC8426398.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            15409..15963
                     /locus_tag="MB219_000664"
     CDS             15409..15963
                     /locus_tag="MB219_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCC8426399.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16448..16762)
                     /locus_tag="MB219_000665"
     CDS             complement(16448..16762)
                     /locus_tag="MB219_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCC8426400.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(16776..17036)
                     /locus_tag="MB219_000666"
     CDS             complement(16776..17036)
                     /locus_tag="MB219_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCC8426401.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            17206..17520
                     /locus_tag="MB219_000667"
     CDS             17206..17520
                     /locus_tag="MB219_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCC8426402.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            17522..19177
                     /locus_tag="MB219_000668"
     CDS             17522..19177
                     /locus_tag="MB219_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCC8426403.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            19260..21395
                     /locus_tag="MB219_000669"
     CDS             19260..21395
                     /locus_tag="MB219_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8426404.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            21570..>21903
                     /locus_tag="MB219_000670"
     CDS             21570..>21903
                     /locus_tag="MB219_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426405.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         7291 a         3689 c         4772 g         6151 t
ORIGIN      
        1 atttattaac tactgaatca ggaacttata tcattttgaa tgcagcagct gatgcatttt
       61 tctatttctt gcctatattt ttggcatata ctgctgcaaa aaagttcaat acagatcgtt
      121 tcatcgctat ggttattgca gcagctctcg tttatcctac gattgtcagt gcatatagtg
      181 atagcataac cttgcgattt ctagggatgc ccgtgattct agcaagatat acaagtacgg
      241 ttattccagc aattttagct gtatgggttc tttcttatat tgagcctaag attcgcaaaa
      301 gcttgcacga aagtattcga aatttattga caccattcat ttgtatcatc gtgatggtcc
      361 caattacttt attagttgta ggtccaattg cagattatgc aagtcagctg attgcagctg
      421 gatatttagc tgtttataac tttagccctg ttttatctgg tgctgttatc ggcggatttt
      481 ggcaagtgtt agtgattttt gggttacatt ggggacttgt accagtaatg accaacaatc
      541 tttctttcta cggtcgtgat acgctaggtc cagcatgtat gactgccgta gctgcgcaag
      601 caggagcagt acttggggta tttttgaaaa cgaaaaataa gaaagtaaaa agtttatctt
      661 tatctgcatt tatcacagca ttatttggaa tcactgaacc tgctgtttat ggtgtgactc
      721 ttaaatataa acgaccattt tacattgctt gtatatgtgg agctatattt ggtggagtag
      781 ctggtgccgc aggagccggt gctttggcag ttgctacaag aagtattttg tctttcccga
      841 tttatattgg tgaaggattt gtttggctag ttgctagtta tttcttagcg atgatcagtt
      901 cctgtatgct aacttttctt ttcggctaca aggatgaaat cgaagaagaa tcctcaaagg
      961 atatagtgtt aagtactcct gcagcaggtg aaataattga tttatctgaa gtaaacgatc
     1021 caacatttgc tagtggatct ttaggagaag ggtttgcgat cattccaacg gacggaaaaa
     1081 tctacagtcc tgtaaacggg gaagtttcaa cagtttttcc aacgaaacat gcaattgggg
     1141 tagtcagtga agaaggagca gagatactga tccatatcgg tattgataca gtgaatttaa
     1201 atggcaaata ctttcaaagt gcagtatctg atggaaaaaa agtacgtaaa ggcgatttat
     1261 tgatggaagt agatttgcaa gaattaatca aagagggata tgatccgacc acaatggtta
     1321 ttgttacaaa tagtagtaga ttcaaaaata tcattactag tcataagaaa aataatcaaa
     1381 caagaaaact agcaagttta gggggatcga tatgaaaaaa tttccagaag gatttttatg
     1441 gggcggagcg acagcggcca atcaatgtga aggtgcatgg gatgtagatg aaaaaggatt
     1501 atctgtgtct gatgtgtata cattcgacag cgatttacct aaagaaaaat ggacagatca
     1561 atggcatatg atgacgcatc agcaagtaaa agcggcgcag gatcccaaca gcaagtatta
     1621 ttatcctaaa agacacggaa atgatttcta tcatcacttt aaagaagaca tccgtttatt
     1681 tgctgaaatg ggttttaaat gctatcggat gtctatcgca tggacaagaa tttttcctca
     1741 tggtgatgaa gaaacaccca atgaagcggg attatttttt tatgatcaag tgtttgacga
     1801 atgtctaaaa tatgggattg agccagtcgt ttctctttcc cattatgaaa tgccgttata
     1861 tttggttaca gaatatggtg gatggcctaa tcgaaagctt attcagtttt acgttcgttt
     1921 cgctgaaaca gtattcaaac gctataaaaa taaagtaaaa tattggatga ctttcaatga
     1981 gatcaactgt gtaaaacatc acccttttgt tagcgtagga gtaattgaag aaaaccatcc
     2041 ccatttggaa caagcgaaat accaaggtgc acaccatcag tttatagcaa gtgctttagc
     2101 tacaaaagct tgtcatgaaa ttatcaaagg ttcacaagtc ggttgtatga tcagctatca
     2161 gcttcttgtt ccgtattctt gtgatccgga tgatattcaa aaaacaattg agcagcaaag
     2221 aacttctttg ttctttagtg atgttcaagc aagaggatat tatccagcgt atacgcaacg
     2281 aatgtttgaa gaaaaaggtg tcaacttaaa aatagaagta ggtgatgaag aaatcttagc
     2341 agcctaccct gttgattttg tttcttttag ctactacatg tctagtgcag tcagtgctca
     2401 tccagaaaat ttggaagggg tccaaggaaa cttaataact ggaggaatta aaaatccgta
     2461 tcttccatca agtgaatggg gctggcagat tgatcctaag ggtttgagaa ttgctttgaa
     2521 tcaattatat gataggtatc aaaaaccact gttcatagct gaaaacggac tcggagctat
     2581 agacaaagta gaagaagatg gaaccgttca agacgattac cgcattgact atttgaagca
     2641 gcatattgag cagatgaagg aagctatcga agatggagta gatttatttg gttatacgtc
     2701 gtggggctgc attgatatcg tcagcgcttc tacttcacaa atgtcaaaac gttatggata
     2761 tatctatgtt gatcaagatg acgaagggaa tggcacaaag aaacggttaa agaaaaaatc
     2821 atttgattgg tacagacaag tcatccaatc aaatggcgaa atcctataat aaaaggaaat
     2881 aagggactgt gacaaaagtc ttgtcacagc cccttcttta cgaataaaca gtattctcct
     2941 gccaactcct cgatcacaag tcaccatagt cataaaacat gagaagctgt tttttgaaat
     3001 tgctccttgt gactcggagt tcaacggcag gctcacgacc tctcaataaa cggtgtacaa
     3061 tcagctgacc atcggcatta gctcaaaaaa cgacaaaata tgaggaacta ttttggtttg
     3121 gctgttctta ttacttgatg caggacggct ttttcacaac ctctgatata aacggtgttc
     3181 aaaagccagt ttttcggtat taagccaaat ttatgcaaaa atgtggaaag ccattttcac
     3241 aaaatttttc ttcatactca aaactaaacg actttttcac aacctctgat tcacggtgtt
     3301 ccagaaggag ttttgtcgaa aacaagccga aattctttga agatggctac ttcttttttt
     3361 caaatctaga cagaggaggc ggacattatt ccgtgttttt tgacaaaaaa taaaaatcat
     3421 actatgatat gtacgccaat ggaggggatg aaatggaaaa gccaattgaa aaagcgttag
     3481 atgaatgtct tttctttagc gtgaaaaagc tagatcgcat gttgaataaa ttagctgatg
     3541 aagcgtttag aaggacaggt ctggcaccaa cttatggttt tattttattg atattaaaag
     3601 aaaaagatgg aataccacaa aaagatattg cacaaatgct ctattccgcc ccatcaacga
     3661 ttgctcgttt tgttgaaaaa ttagaatata aaggatacgt caaaacagtt agcgaaggtc
     3721 gcttgtcatt agtttatctt acagaacaag gacgccaatt tgctaaggaa gtcgatggta
     3781 gctgggaaga acttcatcag tcatacaaag cagttttggg aaatgaagca agtaaagaat
     3841 tagcccagac aatgaatgat gcaacagata agttacaaaa aaaataaaat tttttaagca
     3901 aattagttgc atgtacaact aatcgagtgg tttaatgata gatgattctc gataggttaa
     3961 tcagagcgaa ttatgggagg aatgacaatg tcacaatttg atcaatcaat tactttacct
     4021 tcaggagttc aattgaagaa ccgattgatg ctgtcgccta tgacgacaca actcagcttt
     4081 ttcaatggag tgatcaccga agatgagatc acttattata aacaacgttc taaaggtctt
     4141 ggtgctgtta ttactggtgc tgcaaatgtc caagatatcg gaaaaggctg gccaggtgaa
     4201 ctcagcatcg ctcatgatga aatgattcct cgattaagtg agctagcgaa agcaatccaa
     4261 ggagaaggcg caaaagcgat tattcagatt ttccacggtg gacgaatgac aagtcgagca
     4321 actttatctg gagaacagcc tgtatctgca agtgcagttg cagcaaaacg tcctgatgcg
     4381 gaaacaccac gagcaatgag tgaagaagaa atcgttgaaa caatccaagc atttggcaaa
     4441 gccacacgta gagcgattga atcaggattt gatggaatcg agttgcatgg agcaaatacg
     4501 tatttgatcc aacaattttt ctctccgcat tcgaatagac gagaggatca atggggcggt
     4561 agcttggaaa atcgttatcg ttttattgaa gaattattaa caacggtttt ctcagcggtt
     4621 gaacaatatg ctaaaaaacc attcatcgta gggtatcgtt tctcaccaga agaatatgaa
     4681 acaccaggta tccgttttga agacacgatc tggcttcttg aacgattacg tgagtcaaaa
     4741 ctagattatc tgcatgtttc attaaatatg tatgatcgaa tagctcgttc tgataaatac
     4801 aacgataaat cgattcttga agtggtccat aacacgattc aaggaaagat ccctttagtt
     4861 ggtgtcggtg ggatccgcaa tcgcgaagat gtagagcagg tattgaaata tgcagaactt
     4921 gcagcaattg gacagcaaat gatcgtcgat cctgactggg cagagaaatt attggaaaac
     4981 cgtgaggatg aatttgtaac gaaacctttt gaagaagcat ataaagagct ttatttgcca
     5041 agcccattat ataatttctt gaaagcacgt tatcaataac gcaattttta tagaaagagg
     5101 atgggaaaat gaaagtattg atcgtattaa caaatgtaga aaagtacgaa aagatcgagc
     5161 gtcctacagg tttgtggtta agtgaactga ctcattttta tgatgtatta gtaaaaaatg
     5221 ggatcgatgc agattttgtc agtcctaaag gcggatatgt acctttagaa ccgcaaagtg
     5281 ttttgaatat ggatgaaatc gattgggcct attatgaaga tgaagatttc agaaatcggg
     5341 ccttgggaaa taccttacgt cctgatgatg tggatgcaaa tgattatgca gcggtttatt
     5401 atgctggtgg ccacggaaca atgtgggatt ttccagaagc aatagatctt gggaaagtcg
     5461 cttcacaaat ctatcgaaat gaagggctgg tctcagctgt ttgtcacggt gttgtaggat
     5521 tgttgccagt tacagatgag tctggaaaat ctattttagc tggacgtaca gtgactgggt
     5581 tcagtaatga agaagaggaa gcaaatggta caacgaatga agtaccattc ttagcagaag
     5641 atgctttgaa agaaaaagga gcaaactatc agtctggagc agctttttca gaagtggttc
     5701 aaattgatgg gcggcttctt acagggcaaa atcctcaatc tgctcatcaa ttaggcgaag
     5761 cagttgttga agcattaaag aattaaaaat aaatgatgtg tctattctaa ctattacatt
     5821 acacacgcta aaactcgtct tttcgttctt ttctgtttcc tatttggaca gaggtacgta
     5881 aaaggcgagt ttttttcttt tataagagta cattttttga aacatctctt cagaaatgat
     5941 actcttcaat taggtccact agtacattat tgagaagcag acctagaaca cggtatgatg
     6001 gagagggttg attctgctgc atcgctatgt atcataaggt tgaaagcgag ttatgaatac
     6061 tgtgttttct gattgagaag ttagcagtag aacaccttat aagaaagagg aatgcagatg
     6121 aacattgtaa ttgtcggcgg gtcgtttgca gggattcatg cggctattta tcttcgccaa
     6181 ttgatgccaa caagtgaaat atatttgatt gaaaagcaat cgaagttagg atggattccc
     6241 agcgggttca atttgatatt aaaaggaaag gttaccagtg aaaaacaact ttcttggatc
     6301 acgaaagaag aactgactga ccgctatcga atccatgttt acacagaaaa gacagtaata
     6361 ggattgaaag aaaaaaaagt cgttttagca ggaggaaaaa aattggattt tgatcgtttg
     6421 attctagcga ctggttcaaa tcaaaagttt cgaaatattt ctgcagaatc aacgcttatt
     6481 cacccagtta aaaagataca aaacgtaacg gctcttcagc aaaagatcat gaatgcaaag
     6541 aaaattgcaa ttattggtgc aggacaagca ggaatcgaaa tagctgaagg gttggcatca
     6601 agaaaaaaac aaatccatct ttacgaaagc cgaaagtcaa ttctttttcg ttatctagat
     6661 cctgaaatga cagaaccgtt agtaaaggaa atgaaaaatc aagggctttc acttttttta
     6721 gaagaacaag ttgtgtcttt gaccgaaaat gaaaccgcta ttgtagagac ggaaaaaaga
     6781 aaggagaact atgatcttgt tttgctggct aatcattctc gccccgacaa tcagatgtgg
     6841 gaagaacaat taacattgaa tgatgatgga acgatctggg tagatgacta cttgcaaacg
     6901 tctcaaaaag acgtttatgc gattggcgat gcgatccaag taacttttcg tccaacaaat
     6961 gaaaaaatgt atgtttctct agtaaataac gctgtccgta cagcacgaaa tgtcagcaag
     7021 acaattagtg gctcacagac aaaggatcag ggaacctatc gtccagtcgg caatcagtgg
     7081 tttggctatt tcttaggaag cgtaggtttg acagaagaag agagcatttt ttatccccaa
     7141 aaaattgaaa caggatacgt agactacaag cttacagcaa taaatcaaag caaagttaaa
     7201 ctcaaatttc tatttgattc tgaaaaaaag attcttggag cacagctcca aagcaaaaaa
     7261 gagatgttcc accttcttga ccaactgaca attgcagtaa aagaaggatg gacgtttgaa
     7321 cagttagaag aacatgagct atttttccag ccagaatacc gtgtgccaga tatggtgtgg
     7381 tcaaaggtgg cgaatactgc ctatgaagat tgaagcatta ttagaaagaa aagagcagtt
     7441 acagatcctg attttgcgaa accttgttct gcaaggggga acagcctcta tcaatggttt
     7501 gagagagcat gtccaactgt caaaagcctc gtttgatcaa tatttggaag atattgaatt
     7561 gattggccga atgatggaaa aaaaagtaga agtacagcga aatgagtatc aggcgctgct
     7621 tgtgttggac gaagatgtaa gtttggaaaa aattctattg tttttattgc aggaaagctt
     7681 gaagtttaag atgctcgtct atttattaga acatcagcaa gtatccatcg ttcgattagc
     7741 gacagcgttt aatatcagtg agtcttctgt ttttcgaaag atcaaagaat tgaaccagct
     7801 cttagaggaa tttggattac aaatcaaaaa cggtcaatta tacggagaag agctacagat
     7861 acgatatttt tattatgaac tattccaata tatcccggaa gatcagcgac cgcttttttt
     7921 gcaaaataca ccagaaaaaa gaccgttcat tctaggattg gaccgtgtat tagaaacaac
     7981 gtttacggca tcagcagaag cacagattgc ttgctggctc gggattacga aaaaaagact
     8041 attgaatgaa aaaagtacgt acgcaacttt gaaagaaaag aaattactct atcagtcgga
     8101 ccggctttat caagcaatcg atccaatcat cgtgatgcat cttagtcgaa cagcagcaga
     8161 actgaatgta tacgaaacaa tgatgtttta cagctttttt gtttcttttt cgatagtaga
     8221 cgaagaaata ttttatcagt atgatttgac acggagcaaa aagttaccaa ccgctgtttt
     8281 agatacttac attcgggaaa cgatgttatg gcattatcgt cctcgtcgtt tgaaaatcaa
     8341 agaagaaaag gcagtaggct accaaatttc ccagatcaac aatgaatggt ttttctttgt
     8401 tggaaagatc gaagtctatg agcgtgatcg gcttttggaa cagcagcaga aaatgctcgg
     8461 gcattcctta acacagttac tggcgaaatt acaggaaaca gcggtgcagc aattgccgag
     8521 gaaaagagct gaagatagtg agcttagtta cctgatgatc cagtatgcca atgttctttt
     8581 gatgatcgat ttttatattg caaaaccagt agtgattgga atcgatttag aaagtctgcc
     8641 gatttatcgt attgcttttc aacaatatct gatcagagaa ctgaggggaa tcggcggtat
     8701 tgagattgga agttatgaag aaggtaaaga atatgatttg gtgatcacat tttgccagag
     8761 aaacaaaaaa cagtgtgaat attatctgtc agaatttgcg tcaccttacg acattattcg
     8821 attgaaaaga agaatagaga cgctgaaaaa agaaaagaat tgattaaaaa atagcttcat
     8881 aataaaaaac tgtcaaaaaa agaaaaaaag aataaaattt tgacagtttc tttttttaaa
     8941 acagtagtat gataagagta gttaaagaaa gcgtttgcat ataatggagg gaatcacaat
     9001 gacagaatca acgtatatca tggcgattga ccaaggtaca actagctcac gagcaattat
     9061 ctttgacaag aaaggaagac acatcggcag ttctcaaaaa gagttcacgc aatatttccc
     9121 gcaagaaagt tgggtggaac atgatgcaaa tgaaatctgg aattctgtac agtcagttat
     9181 tgccggagcg ttcatcgaat cagggatcaa accaaatcag attgctggga ttggcataac
     9241 gaaccagcgt gaaacaacag taatatggga aaaagataca ggacgtccaa tctatcatgc
     9301 agttgtttgg caatccagac agtcagctgg aattgcagat aacttaaaaa atgaaggcta
     9361 tcaagaattt ttccacgaaa aaacagggtt agtgatcgat gcctatttct cagcaacgaa
     9421 gatacgctgg attcttgatc atgtagaagg cgcgcaagaa cgagctgaaa aaggcgaatt
     9481 gatgtttgga acgatcgatt cttggttagt atggaaactg accgatggac aagcacatgt
     9541 tactgattac tcgaatgcca gtcggacgat gttgttcaat atccatcagc tagattggga
     9601 tcaggagatt ttagatttac taaatattcc tcgagtgatg ttaccaaaag tcacttctaa
     9661 ctcagaagtt tatggttata cgcaaggtta tcatttttat ggaagcgaag caccaatttc
     9721 agggatggca ggagaccagc aagccgcatt attcggacaa atggcattcg aaccaggcat
     9781 ggtcaagaat acttatggta caggttcatt tattgtcatg aataccggtg aaaaaccgca
     9841 attatccaaa aataatttat tgacgacaat tggttacggc ataaatggaa aggtttacta
     9901 tgctttagaa ggaagtattt ttgtggctgg ttcttcgatt caatggctga gagatggact
     9961 tcaaatgttg caaaaagcta gcgattcaga agacgccgcg aaacgctcta caagcgaaga
    10021 tgaggtttat gttgttcctg cattcgttgg gctaggtgct ccttattggg atcaggcagc
    10081 acgaggggct atgtttggct tgacacgtgg tacgacaaaa gaagatatca ttaaagctac
    10141 tttacagtcg attgcttatc aagtgagaga tatcatcgat acgatgcaag atgacactgg
    10201 aatcaagatt cctgtattga aagtagatgg cggtgcagca aacaatgaat atctgatgca
    10261 attccaaaca gatatcttga atgtaccgat ccaacgagca gaaaacttgg aaacaacggc
    10321 gctaggggca gcatttttag caggattagc tgtcggttat tggaaagata cagacgagat
    10381 ccgcgaattt tatgaagctg gcaagctatt tgaagttcaa atggaggaag agcgccgaga
    10441 aaaactttac aacggttgga aaaaagcagt aaaagctacc caagcatttg aataaggagg
    10501 aaatccatat gttttcaaac aagacaagac aagatagcat tcaaaaaatg cagcaagaag
    10561 aattggatct gttgatcatc ggtggcggaa tcactggtgc cggtgtggca gtccagtcag
    10621 cagcatcagg aatcaaaaca ggattgatcg aaatgcagga ttttgcagaa gggacttcct
    10681 ctcgctcgac caaacttgtt catggcggta ttcgttatct gaagacattt gatgtggaag
    10741 tagtagctga cacagttggt gaacgtgcag tcgtacaaag tattgcccca cacatcccaa
    10801 aaccagatcc aatgcttttg ccaatctatg agggcgaagg agcaacaacc ttcaatatgt
    10861 tctcggtcaa agtagcaatg gacctttatg acaaactggc aaatgtgaca ggaaccaaat
    10921 atgagaatta tacgctgaca ccagaagaag tattggaaag agaaccgttt ttgaaaaaag
    10981 aagggctaaa aggcgcgggt gtctatctag atttccgcaa caatgatgcc cgtttagtga
    11041 ttgataatat caaaaaggct gccgaagatg gggcttatct agtaagtaaa atgaaagcgg
    11101 ttggcttttt atatgatggc gatcaaatcg ttggcgtcaa agctcgtgac ctgctgacag
    11161 atgaagtgat cgaaatcaaa gcaaaattag tgatcaatac gagtggtcct tgggtagata
    11221 aagtacgaaa cttgaatttt acgcgtccag tctctcctaa aatgcgtcca actaaaggga
    11281 tccatttagt tgtagatgcg aaaaaactgc ctgtaccgca acccacatac ttcgatacag
    11341 gaaagcaaga tgggcggatg gtttttgcta ttccaagaga aaacaagact tactttggta
    11401 cgacagatac ggattaccaa ggagatttta cggatccgaa agtcacacaa gaagatgtag
    11461 attatctatt ggatgtgatc aatcatcgct atccagaagc aaatatcaca ttgaatgata
    11521 tcgaagcaag ctgggcagga cttcgtccac tgttgattgg taattctggt tctgattaca
    11581 atggtggaga taatggatcg atttcagaca agagcttcaa taaagttgtc gatacagtaa
    11641 gtgaatataa ggaaaacaaa gtttctcgtg ctgaagtaga agacgtgttg aaccatttgg
    11701 aaaacagccg tgatgaaaaa gcaccttcta cgatttccag aggtagttct ttagaaagag
    11761 aaccagatgg tttgctgact ttatctggtg ggaaaatcac cgattaccgt aagatggcag
    11821 aaggagcttt gcgattgata cgccaattat taaaagaaga atacggaata gagacgaaag
    11881 aaatcgactc taaaaaatat cagatttcag gtggaaactt cgatccaact aaattagaag
    11941 aaacagtgac agaattagca aaagaaggag tagcagccgg tttagaggaa gaagatgcta
    12001 cttatatcgc tgatttttac gggactaatg cccgacgtat ctttgaatta gcaaaagaaa
    12061 tggcacctta tcctggtttg agtctcgctg agtcagctcg gttacgttat ggtttagaag
    12121 aagaaatggt tttggcccca ggcgattatc tcattcgtcg tacaaatcat ctgttgtttg
    12181 aacgagatca gcttgatgag atcaagcaac ctgtgatcga tgcaattgct ggatattttg
    12241 aatggacaga agaggagaag gcgcaacaga ctaaacgttt agaagcattg attgcagaat
    12301 cagatctgcg ggaactaaag ggggagaaat aaatgaacag tgatatgact caaatcatgg
    12361 gggagtttat tgggacactg atcctagttt tgcttggaga tggtgtgtgt gcggctgtga
    12421 atttaaacaa aagtaaagca caggcttctg gctggattgt catcgcattt ggttggggac
    12481 tcgctgtaac aatggctgtc tatatttctg gattcatggg cccagctcat ttgaatcctg
    12541 cagtctctct agctatggcg atgacagggg caattagttg gaatcttgtc gttcctttca
    12601 tcattgcgca agttttagga gcttttgcag gggccattct tgtttggctt tcttatttgc
    12661 cacattggaa tgcaacgaaa gatgaaagcg cgattttagg aacgtttgca acaggtcctg
    12721 ctatacggaa ttatccagca aatgtgatca cagaattaat tggtacattt gtattagttc
    12781 ttgggttgct agcttttgga caaaacgaat ttgcacctgg aacgaacgta ttcgctgtag
    12841 gtggattgat cttagccatc ggtctttcat taggagggcc aacaggatat gcaatcaacc
    12901 ctgctcggga ctttggtcct cgattagcac atgcggtatt gccaatagct aataaaggaa
    12961 cttcagattg ggcatattca tgggtaccaa tcgcaggtcc aatgatcgga gcaattatcg
    13021 cggtaggtgt ctattcatta atggtgtaaa tctataaaac atactaaagt ttgacaacca
    13081 aaatgtgtct tgctatagtt aagtggaaaa taaataagac ctagaagaat ggggtatgtt
    13141 ttgtgagtgt agctggtatc taatcagtaa gtcgaatagc caataaacaa agtttatttg
    13201 gtgtgcttat gaaacggtac ctgtcaggaa aacaatatct ttgtattcta ataaggatag
    13261 gacatgactt ttttaagctg tgttctatcc ttttatttat ctctaggccc ttatttattt
    13321 tgggacaggt atctcttcaa gaaaaggaga gaatatggac aacgaaatga ttagtcaatt
    13381 acaatttgat cggatcaaaa aggaaatcca agcaagagca attggaaact atagcaaaaa
    13441 acgaatcagc gatatgacgg tctcgacaaa cctacaaact gtcttggatc gtcaagaaga
    13501 aacaagagaa gcacgcttga tcttagaaag cagtcagcat gttccattta tggggttgcc
    13561 gaggatcgat gcactgacag aacaagtcaa aaaagggttg attctgcagc caaccgattt
    13621 gatcgaatat gcggattttc ttagaagcag tcggatgatc acaaagtttt ttgataagaa
    13681 tcaataccag acacccttgt tgtttgctta tagcaaacat cttcctgatt tgataaatgt
    13741 agaagagttg attgaccaga aaataaaaaa taataaagtc agtgacgacg cgtcacgtaa
    13801 tttgcgtaaa gtacgcaaac aattacaact aatcgaaaaa gaaatccagt caaaattact
    13861 gaaattcttg cgtcatccga aaaacaaaga aatgatccaa gaagcaatga ttgttcaaaa
    13921 aggcgaatac tatacgattc ctatcaaagc cagctataaa aataaaatag atggaacgat
    13981 tatcgatgaa tcaaataaaa gaacgacggt ttttattgaa ccaactgtga tttctaaatt
    14041 gaatgaacac tatcagttgt taaaagctga agaaatcagt gaagaatatc aagtcctggc
    14101 ggcattgact ggtgctatcg cagaaaacga agaagtgatc gatttattga tcgaaacgat
    14161 cacggtgtta gatatcatct ttgctcgggc aaaatttagc cgagaaatca atggaagcac
    14221 acctagaatc aataaatcgg aacatatcat tatcaagcaa ggcagacatc catttttacc
    14281 tgagcatgcg gtaccattgg atgttgaaat cggtaaagac tatcgaggct tgattatcac
    14341 cggagcaaat gccggaggca aaacagtcgt gttgaagaca gtcggtttat tgactttgat
    14401 ggcgatgttc ggtatgcaag ttccggcaaa agaagggaca gagcttgctg tctttgatga
    14461 gattttcgtc gatgtcggtg accaccaaaa tttagaaaat gcgttgagta cattttctgg
    14521 tcatatgcaa aacatcgctg ctattttgaa aaagataaaa aggaatacgt tggtactttt
    14581 agatgagatt ggcagtggaa cagagccaaa tgaaggggct gcactagcga tcgcaatcat
    14641 ggaatctatg tatgagcaag gggcattgat cattgccacg acccattatg gagaaatcaa
    14701 gaaattcgcc ggagatcatg aagattttgt gccggctgct atggctttcg atcgggaagc
    14761 acttcgtcct aaatatcagt tgcgggttgg agaaacagga gaaagccaag ctttgtggat
    14821 tgctcataaa atggatatgt cgatgaaatt gatccatcaa gcagagcgtt acttggcgga
    14881 aaaagcctat cggacacaga aaaaagaatt tcttcctaag acagcagtta caaataggaa
    14941 ggagaataaa aaagaacgcc agctctttgc aaaaggtgat cggatatttg tcaatgaata
    15001 ccaaaaagaa gcgttagttt atgaagatat aggtgaagat acgattgatg tatatttaga
    15061 caaaaaaata atccacgtgc ctcgtcagcg tgttcgtctg gtgcgttcag cagaagatct
    15121 gtacccgaca ggttacgatt tagacagtct gtttatcgat tataaaacac gtaaacggca
    15181 gcgtgaccta gagcgaggat caaaaaaagc tcataaagta ctagtaaaag aaatgcgcaa
    15241 acgacaggaa gaaagaagag taaacgatga aaacagcaag tgattatctc aaattagctc
    15301 ataatatcag tggctgtaaa gaaaaataaa aacagtgaaa tcattggttt tattccccgt
    15361 gtaagctaaa ataaaaagaa aagagtgctg aggaggtagg gcgataaaat gaaattaagc
    15421 acagactggc gtaaagaaat acaaacgatt ccgaatcttt tatcgatatt ccgtattttt
    15481 ttgctgccga tttatcttta ttttgtgctg agacagtcat tttatatagc aggagcggtc
    15541 attgttgtct caggtttgtc tgattatcta gacggggtca ttgctcggag atataatcag
    15601 gtaacagact tagggaaggt gcttgatccg tttgcagata aattgacaca actttttctg
    15661 attttgtcta tggcatggta tcgaccgtgg ctttggctgt tgtttggtct attcttgata
    15721 aaagaaggct ttatgtttgt agctggatta atcgggctat ctaaaaatat caaattaagt
    15781 ggagcgaagt ggtatggcaa agtagcgact gctgtcatct atgttggcat gatccttttg
    15841 ctgcttttcc cagaattacc aacgctgtgg gtgagagtca tttttgccgt catcacttac
    15901 ggtcttttac aatcttttgt cttgtatgct gtcgagtacc gaaagatgtt tcaaagaaaa
    15961 taaaaaaggg gtgtgaccta tgtcgcattc ctttttttga taaatagact cccagtagct
    16021 acttctcgat aataagccaa ccaaaccaaa aacaggagaa gttgtttttg aaattatttc
    16081 ttgtgattcg gagttaaatg gtaggctcac aacctcttgg taaacggtgt tcaatcagct
    16141 gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttcc cgtttttttc
    16201 tctaatactt aggtcaaacc gctgatttca caacctcttc cttaaacggt gttcaaaagc
    16261 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    16321 tgttcttatt acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga
    16381 agtagcttct tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct
    16441 ctactgacta aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga
    16501 cacctgctgg tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac
    16561 gcatagcatt ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg
    16621 cagagtctag ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg
    16681 gagaagcttt tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat
    16741 caatatggaa aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc
    16801 gcttgttcta gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt
    16861 ttcagattat ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt
    16921 aattgagtga tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga
    16981 atttgtccct ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca
    17041 cattctcctc tatttttata attagataat accccttatc ggtattttat gcaaatataa
    17101 aaagtagtga aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa
    17161 tataccccat gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt
    17221 caatcaataa aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg
    17281 ttacttgatg ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat
    17341 gttccattac aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt
    17401 tgtcaatcag ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat
    17461 gtagtgaatg tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa
    17521 gatgaaaatc gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg
    17581 ccgattaatg gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc
    17641 aaattgcggc ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt
    17701 ccaaacgcca gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt
    17761 gacagccatc tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat
    17821 tgaacagtac gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc
    17881 agggataaca gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt
    17941 gatgcatgca ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg
    18001 attggaaatg gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc
    18061 accacatatt cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc
    18121 tcatcaagga gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa
    18181 acgattgcga ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg
    18241 tgttcgtcct gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg
    18301 tatcctagta gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc
    18361 tatcgttgta aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc
    18421 caatcgtcaa ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg
    18481 cggtattgga acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag
    18541 tgaaagaaca gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga
    18601 tcatgcaagc tattatccag aagcaacaga tattttactg aagttgatct tccatccaga
    18661 gactggcgag atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga
    18721 tattttagca acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt
    18781 gacgtatgca ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc
    18841 aatgaacatt gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact
    18901 agcaaaagga aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt
    18961 caaggaagcc aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag
    19021 ccaacaagaa tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat
    19081 cttgaagcag tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac
    19141 tgtccgacct gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc
    19201 gactcttaaa agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta
    19261 tggtcaacac acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa
    19321 ttgataaaaa aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt
    19381 cgttgaaaga aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg
    19441 atactttttc gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga
    19501 aaaacgcaga aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa
    19561 aagtcgttgt agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa
    19621 atttcacaga tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac
    19681 ttttttacaa taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata
    19741 cggttattaa agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg
    19801 atacgtctgt agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca
    19861 atgaaatgaa ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg
    19921 tgaaaagtca aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca
    19981 tgcagcgtat tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc
    20041 tgattctatc acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag
    20101 gtgaaagaaa tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg
    20161 aaatagaagg tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga
    20221 cagaacgttt gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt
    20281 tagatcatcc gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa
    20341 aaacgatcga aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc
    20401 aagcaacgaa aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa
    20461 gtcctgctgt gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg
    20521 gtaaattgat ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac
    20581 aaaaaaaata tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc
    20641 agttgtttga aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac
    20701 gtactgttga tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa
    20761 atgtaccagc atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat
    20821 taggtctatt gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc
    20881 aggcaatctt taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc
    20941 atctgccgat ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag
    21001 agcttttcaa aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc
    21061 aaggaaaaga acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag
    21121 ctgagatttt ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg
    21181 aaacccaaac gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa
    21241 tcattccttt gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg
    21301 aaaacgatgg agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa
    21361 tgaaaagcat tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac
    21421 aactgataaa aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc
    21481 ttaggaaaaa tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac
    21541 gtcgtaacaa aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat
    21601 caattttggt gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa
    21661 cgttagtggc tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc
    21721 cattaacgag accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga
    21781 aaatgctgta tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct
    21841 catttgtatt gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg
    21901 aat
//
LOCUS       DAHEYX010000026        21902 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHEYX010000026 DAHEYX010000000
VERSION     DAHEYX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.026"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            278..1180
                     /locus_tag="MB219_000671"
     CDS             278..1180
                     /locus_tag="MB219_000671"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCC8426406.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1393..3168
                     /locus_tag="MB219_000672"
     CDS             1393..3168
                     /locus_tag="MB219_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCC8426407.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3234..4820)
                     /locus_tag="MB219_000673"
     CDS             complement(3234..4820)
                     /locus_tag="MB219_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCC8426408.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFILLLLTKVGFRKIESLVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            5070..5294
                     /locus_tag="MB219_000674"
     CDS             5070..5294
                     /locus_tag="MB219_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCC8426409.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5436..7187
                     /locus_tag="MB219_000675"
     CDS             5436..7187
                     /locus_tag="MB219_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8426410.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7187..8971
                     /locus_tag="MB219_000676"
     CDS             7187..8971
                     /locus_tag="MB219_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426411.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9297..10148
                     /locus_tag="MB219_000677"
     CDS             9297..10148
                     /locus_tag="MB219_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8426412.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10202..11953
                     /locus_tag="MB219_000678"
     CDS             10202..11953
                     /locus_tag="MB219_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8426413.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            11957..12127
                     /locus_tag="MB219_000679"
     CDS             11957..12127
                     /locus_tag="MB219_000679"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8426414.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(12167..13069)
                     /locus_tag="MB219_000680"
     CDS             complement(12167..13069)
                     /locus_tag="MB219_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCC8426415.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            13300..14703
                     /locus_tag="MB219_000681"
     CDS             13300..14703
                     /locus_tag="MB219_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCC8426416.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            14703..16136
                     /locus_tag="MB219_000682"
     CDS             14703..16136
                     /locus_tag="MB219_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCC8426417.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            16281..17207
                     /gene="trxB"
                     /locus_tag="MB219_000683"
     CDS             16281..17207
                     /gene="trxB"
                     /locus_tag="MB219_000683"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCC8426418.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYGSVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17421..19148
                     /locus_tag="MB219_000684"
     CDS             17421..19148
                     /locus_tag="MB219_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCC8426419.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            19163..19513
                     /locus_tag="MB219_000685"
     CDS             19163..19513
                     /locus_tag="MB219_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCC8426420.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(19605..20465)
                     /locus_tag="MB219_000686"
     CDS             complement(19605..20465)
                     /locus_tag="MB219_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCC8426421.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRVFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            20557..20985
                     /locus_tag="MB219_000687"
     CDS             20557..20985
                     /locus_tag="MB219_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCC8426422.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            21147..21323
                     /locus_tag="MB219_000688"
     CDS             21147..21323
                     /locus_tag="MB219_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCC8426423.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            21350..21796
                     /locus_tag="MB219_000689"
     CDS             21350..21796
                     /locus_tag="MB219_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCC8426424.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7146 a         3812 c         4679 g         6265 t
ORIGIN      
        1 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
       61 cagacaaggc tgtttttggc aggaatttct tttttaaaag agttaaaacg tgagaaaatg
      121 atctttatta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
      181 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
      241 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
      301 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
      361 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
      421 gaatattgat gccggaaaag cgtacatggc agaaaaaggt ctaacaaata tcgtcgttca
      481 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
      541 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
      601 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
      661 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
      721 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
      781 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
      841 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
      901 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccggca
      961 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     1021 aatgtttgac aatatttcga aaatcttgga aactccctat gtaggcaaag ataaaaagaa
     1081 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcgcaaacat tcgatccaga
     1141 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     1201 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtacttt
     1261 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacaaggt
     1321 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     1381 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     1441 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     1501 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     1561 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     1621 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     1681 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatt
     1741 gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc tccgattagt
     1801 tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc ggctgttgag
     1861 atcattatcg cttgtttatt agctatttat taccgcaaat acggttctag cagaaaattg
     1921 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     1981 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     2041 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     2101 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     2161 gtctatcaat ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     2221 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     2281 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     2341 gccgcatctc ctacattcca taccgtttct acccgtattg acgaattata tacgactcac
     2401 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     2461 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     2521 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaacaat caaaaagaaa
     2581 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct gccaatcgta
     2641 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     2701 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     2761 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     2821 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     2881 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     2941 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     3001 gtttttcttt tcggtgtctc atggttttgt tcttatgcgc cagaaaaaaa ggactatttt
     3061 ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat tcatcgtagt aacaagcatg
     3121 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     3181 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     3241 atttcttcta ccgtttcctg caaaagctgt tcatattttt tgtttccttt atataaatca
     3301 ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc gatgtgatca
     3361 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     3421 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     3481 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     3541 ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     3601 ttcaaagcgg catgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     3661 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     3721 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     3781 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     3841 atcaaaatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     3901 gaaggatctt ggacagaacc ggttttgaag acagcgacac ccatgatcaa tagcagtgca
     3961 ttgactagaa aagccattga caactggata ttagaatccc aagtagaaaa acgtacagct
     4021 tgtgctacat cttcttcgtt ttggtgatcg atcttccgag tttgtgaaac tgctgaatga
     4081 agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc agtcagcgga
     4141 gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac tgttagggat aaagccagca
     4201 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     4261 acgaaaatta agcagacaac cagtgattcg atcttacgaa acccgacttt tgtcagtagt
     4321 aagagaatga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     4381 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     4441 aattcagtca agatccataa aatgacacct aatgtcttac ttgtgcgtgc tcggattgcc
     4501 tgtgccaaat ccatttggct cacgattcct aattttgctg ccatatactg taaaagcata
     4561 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     4621 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     4681 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc aactgttcca
     4741 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     4801 ttattggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     4861 cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg cctaattttt ttcatggcgc
     4921 gtcttgctag atgacgaaag aaattttgtt ctcctctaaa aaaaccacta ttttttgcat
     4981 gaaactaaag aatacgcctt tcttttttca ataaacttta gtaaaataag tcattgagag
     5041 caattcggaa atagaaagga gagatttata tgttatcaac aggtattgtc gtacttattg
     5101 ctattattgc tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga
     5161 aagactattt agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt
     5221 caatggggca aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc
     5281 aagggaaaaa ataggttctg acccaacgac tttttacggg ctgatttttt cagtccgttt
     5341 tttatttcgt aatttgttag actattcaga ttattcagca aaaaagacaa gcacagacaa
     5401 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aaattaggct
     5461 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
     5521 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag atcgccgata
     5581 aaaatttatc aataaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
     5641 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcgattgg
     5701 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
     5761 aaaaatatcg gactggggat ttgatggctc acgcaacgaa tgatttgaat gcgatccaga
     5821 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
     5881 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
     5941 ctttgctggc cgtcacttca cgagtgctgg ggtcaaaact ccatgatgct tttcgtgatt
     6001 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
     6061 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
     6121 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
     6181 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
     6241 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
     6301 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
     6361 atcgcgtcaa tgaacttttg catgaaaaga cgcatatcgt ggaacaactg ggtgcagtgc
     6421 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
     6481 aatcaccagc attagaacaa atcaagtttg cgatccaaga aggagaaaca ttaggtattg
     6541 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
     6601 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
     6661 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
     6721 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
     6781 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
     6841 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
     6901 tcaaaaatcc tgaattatta atattagatg atgcactctc tgcagtagat gccaaaacag
     6961 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcattgcag
     7021 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
     7081 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
     7141 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
     7201 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
     7261 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
     7321 tttgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
     7381 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
     7441 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcctcatt
     7501 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
     7561 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
     7621 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
     7681 ttcattcctt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttttt
     7741 gccgatttta ggaatcgtca tctggtatta ccagaaattc agttctcgta tttaccgaaa
     7801 tatgcgggaa aaattaagtc agctgaacac gaaattgaat gaatcgattt ccggcatgcg
     7861 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
     7921 tgagtattta gacacgcgtt atgcgatgat caaaacgaat tcgattctgt taagtccgat
     7981 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
     8041 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
     8101 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
     8161 tggtagtcgt atcttgaaga ttatggatac ggaagaatat gcaccacaac aaaaccctgg
     8221 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
     8281 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
     8341 attagtggga cacaccggaa gcggaaaaag ctcgatcatc aacgtattga tgcggtttta
     8401 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
     8461 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
     8521 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
     8581 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
     8641 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
     8701 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
     8761 aacagaagga ttgatccaag aaggattggc aaaaatgcgt caaggacgga caacgattgc
     8821 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
     8881 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
     8941 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtaga attttgacct
     9001 aagcactaga gtgaaaataa aacatagctt ctcatatttt tcatctgtga gagttagtgt
     9061 cgtagtcacc agatttaaaa aaagattcga cagctagtca gaagtgccta atagccacta
     9121 agaaagcgtt gacaaaagca tttgctttcg ataatataaa gaaaacgata caacttggat
     9181 agtgattgcc tgatgcaagc taaacctgat gagtctccca tggtttcatg gacggctttt
     9241 tgggtttttc tttttgtttt ggatacagcc ctatacatca tggaaaggag caaaacatgc
     9301 tgattgagaa agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa
     9361 tgatcgtgat gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact
     9421 catgcatcga caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat
     9481 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
     9541 aagaggctct acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt
     9601 atacggctat cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata
     9661 tcaaacaact atttcctaaa gagtatgcca tcgggaaaaa aacagtgaag aaaatctcgg
     9721 ataaatacca catcaagtta tcagaagatg aagccggatt tatcgcattg catttagtca
     9781 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttactata acaatacagg
     9841 atattctaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccatt
     9901 tttttcgatt cgtcacacac ttacgttttt ttctgacacg cgtcctagat ggagaaaaat
     9961 cagaagtggg agaagtttct gaggaattgt ttcaggttat tcaaaaaaaa tataaggata
    10021 cgtttggttg cgttgagaaa atagcagact ttttagccga gacgttcaac taccagatgt
    10081 ccacggacga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    10141 gtaaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
    10201 gttggctaaa ccttccacta ataaattgga gggaatagaa atgggaaaat accaagaatt
    10261 agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg
    10321 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
    10381 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcattgg
    10441 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
    10501 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
    10561 tttccagcca ttccttgcta tcctagctgc tggcgggatg attcgtggac tgacagcatt
    10621 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
    10681 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
    10741 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
    10801 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
    10861 ttttgaagca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
    10921 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
    10981 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
    11041 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
    11101 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
    11161 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
    11221 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
    11281 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
    11341 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
    11401 gtttacatta ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg
    11461 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
    11521 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
    11581 gatcgccatt gcgatggtgg tatcctttac gctgactttt ttcttttgga aggatgaagc
    11641 aggtgaaaca gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca
    11701 aggacaaatc gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg
    11761 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
    11821 catgaccctg ttcccgacga aacaggcaat tggaatggtt tctgaaacag ggctggaatt
    11881 gctgattcat gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt
    11941 gcagcaagga taaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat
    12001 cgaaaaggaa ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt
    12061 ggatattttg gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact
    12121 gacctgaacg gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt
    12181 tattttcaaa attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt
    12241 tttgaccgac agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac
    12301 tatataacac gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg
    12361 aatacagtcg ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg
    12421 aatcaccgat actggtcaaa gctaaaattg gaacatgatt ttgtttcaat atttctgcgg
    12481 tacgaaggat cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg
    12541 tgcaattgta cgcttcatag aatgattcac caaaagtggt gacaatcgtg acatccattt
    12601 tgatgcgctt cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga
    12661 aaatcacgat tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat
    12721 gaatgagaga aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga
    12781 tcgtcataat accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc
    12841 cttcaagata ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac
    12901 ccattttttt tgctacacgg atgagtgtag aggggtggac ataacatcgc tcagcaatcg
    12961 cttggatggt catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat
    13021 tttctgaagg agaaaaggag atttctttga ttttttcacg taataacatg tagatacctt
    13081 cttcttattt ttttacgtaa attataactt ttttgaaaat gatttgtaaa cggatttata
    13141 ctatataact taaaatagac attttttgac aagatattta caaaaatcca ctagaaaaca
    13201 gtaaattttg taaatgttac tggaaagaaa cagatttcta ttgtttgttt tgtttacaaa
    13261 aaagataatg aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
    13321 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
    13381 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
    13441 cttgagatcg ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaag
    13501 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
    13561 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
    13621 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
    13681 gtcgttttgg caatcgttga ggacatgaag cgtttatgtc caaatgcttg gctgatcaat
    13741 ttcgcgaacc caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
    13801 gtgattggat tatgcaatgt gccagtgatg gcgatgatga cagaaccagc aatgcttggc
    13861 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa
    13921 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
    13981 gagaatggat taccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa
    14041 atgaacatga tcccttgtgg ctaccaccgc tattactatc gtgaagaaga aatgctggaa
    14101 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
    14161 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
    14221 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
    14281 aataaagaaa cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg
    14341 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
    14401 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
    14461 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
    14521 aatccactta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
    14581 caccagcagt atttaccgca attcaaggag acaatcagcc gattaaaaga agaaggaatc
    14641 gaagttcaag atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta
    14701 taatgggatt cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg
    14761 gcggttatga tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag
    14821 accgttttcc tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt
    14881 atccagcaaa aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg
    14941 cagaaatggg gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg
    15001 gggatgagaa agagccgaat gaagctggct tgaaatttta tgaggatgcc tttaaagaat
    15061 gccaaaagca cggaattgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt
    15121 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
    15181 gtcgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggctgaca ttcaatgaga
    15241 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa aaaggagaaa
    15301 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
    15361 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
    15421 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
    15481 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
    15541 aaatggagcg aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag
    15601 aacatactgt tgattttatc tctttttctt attatgcttc ccgagtttcg actaccgaca
    15661 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc
    15721 aagcaagtga atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata
    15781 tttatgatcg ttatcaaaaa ccattgttta ttgtagaaaa cggcttaggt gcagtcgata
    15841 cgccagatga aaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata
    15901 tccaggcgat gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt
    15961 ggggatgtat tgatttagta tcagctggta ccggtgagat gaagaaaagg tacggattca
    16021 tttatgtcga tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat
    16081 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa
    16141 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
    16201 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
    16261 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
    16321 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg
    16381 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga
    16441 tcatgggccc cgatctagct tataagatgt atggaagtgt ctcaagattt gggacagaaa
    16501 atgcttatgg aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg
    16561 aagataaaag ttacgaagga aaaacagtca ttatcgcaac aggatgtatc caccgtaaat
    16621 taggcgtacc tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg
    16681 atggtgcgtt tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag
    16741 aagaagccat ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg
    16801 aattacgtgc acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta
    16861 tgtgggatac agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag
    16921 cacgtaatgt caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg
    16981 ttggactgga tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct
    17041 ggattccaac agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg
    17101 ttcgtgaaaa agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac
    17161 aacaagtgta taaatacttg gagtctttag aagaaacaac agcataagtt taaaaagagt
    17221 agaagaacgg gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg
    17281 atttatgcga acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt
    17341 tttggtcctc atactttcat atctaataag tgaatgctat acttattaag aaccatattt
    17401 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
    17461 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
    17521 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggtatgcgg ggaatcttag
    17581 gacctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac
    17641 gtttcatgga tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
    17701 cacgccatca gtcaccagaa tttgcaatgg aagcagccaa aacactagca catcacaata
    17761 tcccgtctta tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc
    17821 atttgaagac gtttgcggga atcatggtaa ccgcatctca caacccagcg gcttacaatg
    17881 gatataaagt ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga
    17941 cgaaatatgt ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag
    18001 ctaaacatag cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag
    18061 aaatcaaagc agtgacgatt gatcgtgaat tggttgaaac aatggggaaa gatttgaaac
    18121 ttgtgtacac gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag
    18181 ctggattcga acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta
    18241 cggtcaaatc gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag
    18301 aaaaagaagg agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag
    18361 cggttcgttt gccagatggt tcttatgaag tattgactgg gaaccaaatc ggagcattgt
    18421 ttgctcaata tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg
    18481 tattgaaatc aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta
    18541 ccatgttcaa tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caatttgaag
    18601 aagaccattc acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac
    18661 cttttgttcg cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt
    18721 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
    18781 gctatttcgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
    18841 tctctgcttt gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag
    18901 ttattcaaac agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa
    18961 aactaacaac accgccatcc gacgtattga agtatacatt ggaagaaaat ggctggatcg
    19021 ctatccgccc ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagct
    19081 ctacagaaga cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag
    19141 aagaataaaa aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt
    19201 caagttggag gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca
    19261 attattcaaa atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg
    19321 ggaacaaaat gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca
    19381 tcaactacgt ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat
    19441 tttgtatagt cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt
    19501 ggaacatcaa taacgatagg tgaaaataga taaacgctgg agtgtgacaa cgaagaaatt
    19561 tcgttgtcac actccagtgt tttttaagaa aaagacaaaa atgattaatc gcttttttct
    19621 gttgtttcag ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa
    19681 gccgtttctt caatagataa agatgccaca ttgatcacta cacatcctag tttttcataa
    19741 agatcattcg caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca
    19801 gggtttaatc cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta
    19861 ttcgttagtc ccacgatttt tttaggatcg atttcccaca gctgtttggg aatatgtgct
    19921 tggggaatca aaggtaaatt agcaactttt aaatttttat ttgctaaaaa caagctcaaa
    19981 ggcgttttgg aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaacacga
    20041 ggatctttcc catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag
    20101 ttctcattca ataaatggag ggcaccaggt tgccgaattg gcttgatacc agttaaacgt
    20161 tcaatttctg atatcggtgg cgtcatcaca tcgaaactaa ataattgatg ttcttcacaa
    20221 aaagaacgtg ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt
    20281 tcttctaatg catcttgcaa tgcctgcctt aatgtttttt catctgtcac gaacgttctg
    20341 cggaataggt tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc
    20401 gcagtttctc cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa
    20461 gacatggata aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc
    20521 gtacttcgta atcattttga tttggaggaa taagtcatga aaaaacagac attgatcgaa
    20581 caatcattag aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg
    20641 ttaacttatt tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg
    20701 aatcaggaat tcaaaggggt aagttatgat acgatttacc ggaatctcca tgattttgca
    20761 gagatcgatt tactggaaga aaccgattta aatggtgaaa tgaagtttcg ttttcattgc
    20821 tgtcaggggg aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa
    20881 gaaatccata tgtgtccaat gaattatttc gaagaacagc taggtggatg cacgatcgaa
    20941 gggcatagat tcgagatttt cggaagatgt gaaaattgtc aataaggatt taaataaaat
    21001 aacatttgat aatttaaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata
    21061 taatatttac aggacagtct tgttttttga gatatatttc aataacaatt acggttactt
    21121 tttaagctcg gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac
    21181 ttgacgatgc tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat
    21241 ctcgtaaacg tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag
    21301 ctagaaaacg caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact
    21361 aagtagatta aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca
    21421 agtcatccga atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt
    21481 gacagaagaa gaagaactaa cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt
    21541 gcatgagttt gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt
    21601 aatagtcgaa aaatatatgc cggaacaact ttccgacgaa gaaattcgac aacttgttca
    21661 agaagcaatc actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc
    21721 aattatgcct aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga
    21781 actattacaa tcttaatttt atcatttaaa ctgcggacag tgattgtccg cagttttttg
    21841 tgttcgtaaa aaaagaacta aaaaacagct agaagaaaga ataagattct ttcttctagc
    21901 tg
//
LOCUS       DAHEYX010000027        20970 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHEYX010000027 DAHEYX010000000
VERSION     DAHEYX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.027"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(17..1027)
                     /locus_tag="MB219_000690"
     CDS             complement(17..1027)
                     /locus_tag="MB219_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCC8426425.1"
                     /translation="MKKKIFVGALVALFLLPVNAFAYTINNEFNLGANEGSSQVSNNQ
                     YILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKETFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVNANGVSRPD
                     VNQSYSTYGNVGYHVSFNMRNFSNKKVYVMMRATNDPEGNTKDGAQDFHDKRWYLNIP
                     KR"
     gene            complement(1038..1235)
                     /locus_tag="MB219_000691"
     CDS             complement(1038..1235)
                     /locus_tag="MB219_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCC8426426.1"
                     /translation="MILPDKYYQIIKWAVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(1249..1491)
                     /locus_tag="MB219_000692"
     CDS             complement(1249..1491)
                     /locus_tag="MB219_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCC8426427.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            complement(1526..1663)
                     /locus_tag="MB219_000693"
     CDS             complement(1526..1663)
                     /locus_tag="MB219_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCC8426428.1"
                     /translation="MFSFSDIKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(1663..2070)
                     /locus_tag="MB219_000694"
     CDS             complement(1663..2070)
                     /locus_tag="MB219_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426429.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQNYSKKLELLENLSIGLQVDNTALKKSNEELTEK
                     ADSMAQLNAKLMFNDLTINKKIEAIEKQIGGAS"
     gene            complement(2098..2622)
                     /locus_tag="MB219_000695"
     CDS             complement(2098..2622)
                     /locus_tag="MB219_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426430.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPYGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            complement(2622..2990)
                     /locus_tag="MB219_000696"
     CDS             complement(2622..2990)
                     /locus_tag="MB219_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426431.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTIASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            complement(2995..3783)
                     /locus_tag="MB219_000697"
     CDS             complement(2995..3783)
                     /locus_tag="MB219_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426432.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITSEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NAEVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVSQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            complement(3783..4691)
                     /locus_tag="MB219_000698"
     CDS             complement(3783..4691)
                     /locus_tag="MB219_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCC8426433.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYRDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMTAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(4657..5175)
                     /locus_tag="MB219_000699"
     CDS             complement(4657..5175)
                     /locus_tag="MB219_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCC8426434.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTQ
                     YEIKVRHGNVFSAAEIGRAYPWSLYLWTVIAHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            complement(5186..8008)
                     /locus_tag="MB219_000700"
     CDS             complement(5186..8008)
                     /locus_tag="MB219_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCC8426435.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLIGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFDIAMTSKGEIVANFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKAGYKTATRGTLWYTGSMAADAR
                     GARQYLTEISPNEAKAGDIIIVNLGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            complement(8005..8709)
                     /locus_tag="MB219_000701"
     CDS             complement(8005..8709)
                     /locus_tag="MB219_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCC8426436.1"
                     /translation="MNPDEPNFIWKNRNAVIDMDCIIEVELPDIMPNKRYETYTVQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAIGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(8721..11030)
                     /locus_tag="MB219_000702"
     CDS             complement(8721..11030)
                     /locus_tag="MB219_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCC8426437.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAASLAAFGEVDEALDTIITKTGATGKVADSLGESFKKVGANTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAVMARQAIEAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFNEGVTLMGQFEQAGVDSGAALSSL
                     SKATVAYAKDGKTLSQGMGELQDKIKNASSETDAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKIADESQGSVGETFEATLDPIDKSNQAMNNAKLALADVGESVQISLLPFFE
                     MAIDALKSFKGWWDSLDQGTKNWIITLAGIAAVIGPALVVIGTLMSSVTKITAGVKDL
                     ATVWSGLGKLFGLSGGWFAVAVIAIAALVAGLVWAYNNVEWFRNGVNAFFQGVSDIAV
                     EVFNFMGGYISNIFGGIIQNFQNFFDAGKRIFTGFIDFITGIFTGDWERAWNGVVNIF
                     GGIFDGIAAMAKAPFNGMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(11225..11689)
                     /locus_tag="MB219_000703"
     CDS             complement(11225..11689)
                     /locus_tag="MB219_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426438.1"
                     /translation="MARLADYGIHVDDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(11689..12315)
                     /locus_tag="MB219_000704"
     CDS             complement(11689..12315)
                     /locus_tag="MB219_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCC8426439.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWETLAKVGGGK"
     gene            complement(12322..12687)
                     /locus_tag="MB219_000705"
     CDS             complement(12322..12687)
                     /locus_tag="MB219_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCC8426440.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNQRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(12677..13015)
                     /locus_tag="MB219_000706"
     CDS             complement(12677..13015)
                     /locus_tag="MB219_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426441.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQISFFTLGTEQDLAVIEEALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCMENAK"
     gene            complement(13005..13331)
                     /locus_tag="MB219_000707"
     CDS             complement(13005..13331)
                     /locus_tag="MB219_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCC8426442.1"
                     /translation="MPKRQTNNLRWKAELLEIKTGTDENDRPTTVYESKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDTREMEL
                     SLAYVD"
     gene            complement(13309..13590)
                     /locus_tag="MB219_000708"
     CDS             complement(13309..13590)
                     /locus_tag="MB219_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCC8426443.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKALSAITPFIVQGVIQNAETTDQ"
     gene            complement(13592..14956)
                     /locus_tag="MB219_000709"
     CDS             complement(13592..14956)
                     /locus_tag="MB219_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCC8426444.1"
                     /translation="MNKKLLKKLQARHEQRLTELRGRIESGEVREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(14968..15540)
                     /locus_tag="MB219_000710"
     CDS             complement(14968..15540)
                     /locus_tag="MB219_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCC8426445.1"
                     /translation="MKKKEIRTFDITNLSTRDVTENNSRIVTGYAAVFNSRTLLWEGL
                     EEVISPGAFSKALSNSDVRCLFDHDWGKVLGRTKSGTLRLEEDERGLKFEVELPNTTD
                     ANNLIESMSRGDIDQCSFGFIPTEETWDYNTDPVLRTLNEVELFEVSIVSLPAYQDTE
                     AALARSKQEVQQDIATRKKMIQKINGALNA"
     gene            complement(15512..16699)
                     /locus_tag="MB219_000711"
     CDS             complement(15512..16699)
                     /locus_tag="MB219_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCC8426446.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNVKGGDGNEKEGN
                     SDI"
     gene            complement(16763..18490)
                     /locus_tag="MB219_000712"
     CDS             complement(16763..18490)
                     /locus_tag="MB219_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCC8426447.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(18487..18939)
                     /locus_tag="MB219_000713"
     CDS             complement(18487..18939)
                     /locus_tag="MB219_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCC8426448.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(19051..19431)
                     /locus_tag="MB219_000714"
     CDS             complement(19051..19431)
                     /locus_tag="MB219_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCC8426449.1"
                     /translation="MKYCQFDGCTNKIAKGIYCAEHKRSSKSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(19428..19814)
                     /locus_tag="MB219_000715"
     CDS             complement(19428..19814)
                     /locus_tag="MB219_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426450.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAVRAKGDIINLIREAE"
     gene            complement(19795..20100)
                     /locus_tag="MB219_000716"
     CDS             complement(19795..20100)
                     /locus_tag="MB219_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426451.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            20744..20920
                     /locus_tag="MB219_000717"
     CDS             20744..20920
                     /locus_tag="MB219_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426452.1"
                     /translation="MLTNEQRAHDLAIATLPFVRDIVKSQIIEGKDAKFDAYFEYIKL
                     YNQFLSAVSEDFKN"
BASE COUNT         5739 a         4672 c         3418 g         7141 t
ORIGIN      
        1 atcgaggggc tattttttat cgtttcggaa tattcaaata ccaccgttta tcgtgaaaat
       61 cttgcgctcc atctttagtg tttccttctg gatcattagt tgctcgcatc atgacgtata
      121 ctttcttatt agagaagtta cgcatgttaa aagatacatg ataaccaacg tttccataag
      181 tactataaga ttggtttaca tctggacgtg aaacgccatt agcatttact cgtgctaact
      241 cttttccagt attatagtcc atgataaaaa tgtactcgta tttatagtta gcaatgtgcc
      301 agccagccac atgcaagttt gcgttttcga tttctccaaa ctgatcaatg tgggcgtaat
      361 ttgttccatc agtgagtgta ggatttgctg cacctgctcg tgttgggtca atgacaggct
      421 tgttctcaga agttgttgga ttttcatctg taaatccatg ggctaaatcg taagccagtt
      481 tttccttact cacacccatt tctgacaagt atccataagg atcagtgtga tcgccccaaa
      541 tgttttgagt tacccataaa tgtgatttga ttcctggttg gttataaggc gtgtccaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacatat gctttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggactgttag
      721 cattagcata cgaaccagca ccgtactgta cataaccagg ttgtccaact tggtaaacaa
      781 ttccaccgtc tcccacaata taagcagtat aagcactagt ccatgaacgt tgcatatatt
      841 gcgcttcatt gcgtcctgtc gcagtttcgt ttgctgtttc atgcaataga atatattggt
      901 tatttgatac ttgagaacta ccttcatttg cacctaaatt aaattcattg ttgatcgtat
      961 aggcaaatgc gtttaccggc aacaaaaaaa gagctacgag agctcctacg aagattttct
     1021 ttttcatttg tttcctccta ttttttcaaa ttataagccg acacaccagt gataacgcct
     1081 aaaaatgttg ctgctgcatt gatagtcaga actgtcatat ctgttccatt ccatccatat
     1141 gcttttccta acgtggctac taaaacagat gcagctggta atactgttaa aactgcccat
     1201 ttaatgattt gataatactt atctggtaaa atcatttctt cgcttccttt atagtttagt
     1261 taggaaatag ccaatgattg taatgcctaa gccaatcata tagccccaag cccatttatt
     1321 attgttcttc atttccttga tgtcttctgc attgtttagt gctactgaat aggctttatc
     1381 tgccaaatct tttgcactat cagccttttc tctaagtgat tcgtagttgt ctaattttgt
     1441 ttcaattctt actaatctct ccacaacgtc ttggagcgct tcttctttca tgttccacca
     1501 actttccaac aaaaaaacgc atcacttaag atgcgctctc ttctttgcta ataattttat
     1561 ctgcttcttc gtctgtaatg catagtggaa cgaataatcg aacttgatcg tcagtaaaac
     1621 agccccaatc atacatcatt tttatatcgc taaaactaaa cattatgaag cacctccaat
     1681 ttgtttttct attgcttcaa tttttttatt aattgttaaa tcattaaaca tcagtttggc
     1741 gttcaactgc gccatcgaat ctgctttttc cgttaactct tcatttgatt ttttcaatgc
     1801 agtattatct acctgtaaac ctatagataa attttctagt agttctaatt ttttagaata
     1861 attttgtgta actgcttctt cccacttatt ttcagtgaag ttaaaaaatt gcgactgttg
     1921 tcttcttccg aaaccttcat ccgtttcatc ttccttcttt tcaaacaaaa taggtggaac
     1981 ttcgacaaat ggtaaagatg ttggaaagtt atcttctact tgatgttctt catatcccat
     2041 tggatacaat actttataaa ttgttttcat tttactcttc ctccaattag taatctgtta
     2101 agccggccaa ggatcttgag tgatccacat acctgaaata tatgaactac ctgaacctga
     2161 tttggcttgt atgatgctac tttgatcaat gaatgcacgt gcatccgctg gctgacttgc
     2221 atttctaact agtgaaagcg cggtttgcgc tggatagcct tgatcacgtt tgaaaccgta
     2281 cgggattttt aaaatccaac gagtttctga tccttcgggc catgtgccac atttaaaatt
     2341 aaaagtaaga aatacgatgt ttccgattct gattattttt ccattcacat cagttacgtt
     2401 tgttgtatca cttcgatcag ctagtgtgat agctcgctcg atcatacctg cttgcacagg
     2461 caatccattg aattgcaatc cgtctttaaa atttttcgtt cctaagaccg tttcattacc
     2521 tgtggctttt actaaaacac cctccacacc atcaattcct ttggcatgag ttttcaaata
     2581 cttagcaact ccgtcttctt ttaattgcac aatatcagcc attaaaccgt ccccactttc
     2641 tcaaatgtaa tattggctaa tccatcgagt ttcactttat cttctttgga catcaaacca
     2701 tttgctgtcg tcgtagccac agctgtcgtt gttgcattgg ctccaggatc gcctttgtcc
     2761 cctttatcac cttttggcaa tacaaaatta aactttgctg cacttgcagt accagcattt
     2821 gtgacactag ctattgtacc actcgttact gtccctacag taattgtggc agcctgtcca
     2881 gggtctcctt tatctccctt gatagtttcg ggtttaccct caacagcatc ccaatgggtt
     2941 tgagggtaaa cctgaacatc attctgtttt acttttacaa tatctgtcat ttgtctatac
     3001 ctcccctact ttttcaaacg atacatttgg tattttggaa tctgtataag cttgtgcatt
     3061 ttgataagct tcactcattt tttgatcaac atattcttga gagacgccgc caccttctcc
     3121 accacctgtt gcagagatta ctcctgtgtt tgaaatactg atattagctc cggcagtata
     3181 gttgaattgc ttatcggccg ataaaacatc atcagtcatc gttaaacctt caccgacacg
     3241 aataccacct tttacaaatt cggaggcagt gggtaaaacg taaaaaccag tcacttctgc
     3301 atttattttt ccatcttcat caatagtgat gttttctcct gcagtatagt ttttcagtga
     3361 atcgagtttt tcttttaact ccagcgaaaa attgacatca gattgtctga ctgcagagac
     3421 ttttcctgtc tcatcaatcg tcaataattc accaattttt atgccaccca aacggtcgat
     3481 agtggcaact ggcaacacgt agtcaccttc tccactgttt aaaatttttt gatacatttc
     3541 tgacgtgata atgccgtcat cttcttcact tgcatagggt aattctgtga gaacattctc
     3601 taatcccaaa tcttctttag taatgacgac ggctccaatt ttgccattaa cagataaaac
     3661 tgttgcttct cctgctatta tttcttctaa cccaagaact gcggaagcat gtgtgattgg
     3721 aaagaattgg cgttgtacac ctgttttttc atccgtttcc atcattcgtt tagttttagc
     3781 cattagatca ccccaacttt ctcaagagtg aacacatttt gctttggatc atcaacggtt
     3841 gcgatgatca aggctccttc ttcaatcggg aattctacag ttccaacttt ttcgacttcg
     3901 tgattatctg aaaagagatc atcctgcaaa atatcagtca cttcaatctc accgtattca
     3961 atcgtgaaaa gtgttgctcg taatttctga tatagataat ccatatctgc cagcaagcgc
     4021 tctgaaatag acgcatgacg aactccttga atatcaacac gtgcatccat taattcggct
     4081 aacattactc cacctggatc gatagacttc aaaatatctt tgattgattc aaaccatttt
     4141 aaataatcgg tttcttgtcc ttctctccat gcttcaaatg tattttgttg atcttgtcgc
     4201 cattgttcaa attctttttt gcgtgcattc atccaatctg tgaagtcgcc tttgttttca
     4261 ttgataaaag ctgtcatatc cgcaattaaa tcttcgactg tctgccaata cgatcccatt
     4321 tcaccctctg tttttgagac ggctttaatc acaaaatagg agaagtcttg cgtcgtagca
     4381 attaattcat catctctata aatgataaaa tctgctgttt ggcgatgtag gcattgcatc
     4441 gaatattgat caaatgtgta ctgaattttt cctttttttg catcaatgac tttagtacct
     4501 aattgaactg ggtatttgtc tccgacaact gattcaaaat agactttaca accagttaga
     4561 tcatagggca ttccattttc agtgatggtt gcttccataa cttcggaatt cttattgcct
     4621 tgccgtacct gaatcattcc gacatagtta taaggctcag ttgtgctgag tactacgttc
     4681 cactttgcca ttaattacca ctcctttctc aaaatcaggc gggatacaaa tagaggctat
     4741 ctgaccagat ccgaagaatt cacggtcata ttctgccaca attccacctg attctgcatt
     4801 ttgttcatag gtttgaatac gcccattttc cagacctttg attacacctg tgtggccgta
     4861 atattcatgg ctttcaaatg tatcactgat ttttgcgttt ctttcccaat tgataattga
     4921 gccaacaact agctggtcat actcaggatg agcgatgacc gtccataaat ataatgacca
     4981 tggataggct cgtccaattt cagcagcgga aaatacattg ccatgacgta ctttaatctc
     5041 gtattgtgta cctgccccca tgtcaggacc aatcatcaca ccggcatatt ccgcagataa
     5101 tgcataacat tgatggtttc cgataggttg attcaacaaa cttttcaaat gatttaatcc
     5161 tttttcttcg atcacaaaat cacctctatt tcttcgctct ggcgagacaa acatcgccgc
     5221 catttaataa atacccgaag gaccaatcga cgcgaccgat accaacacca ttggaattca
     5281 tgccacccat ttcaacaata gaagtgctat atcccttcca atcctctgct aaaatagcgg
     5341 tatgtccatc attaccagca ccagcaccta aattaacaat gatgatatct ccggctttcg
     5401 cttcatttgg agatatttca gtcagatact gacgtgcccc tcttgcatct gcggccattg
     5461 aacctgtgta ccacagagtc ccacgtgttg cggttttata tcctgctttt gtcaaagcca
     5521 accacacaaa agaagagcag tcagcatacc cattacgatc aggattctcg acgctaccaa
     5581 aattccaacg taacggttgc gagtaatgga aataacccat caagcttttg gctgtactga
     5641 gaatactgcc agcagatggg acttgaagat ctttgaattt gttataccaa ttaactgccc
     5701 atccttgtct ttctggatga gttgtggctg gtctttcata gtttcgttca aatgtataag
     5761 cagcagctgc tggatcagtc attgctttaa atccagaaac acttgccggt tgaacaattc
     5821 ctaaccattg accattatac attgtccaat ctaataactt tccttgagct gacattgttc
     5881 gataatcttc tgtaattcct gcagctctca tcaggttttg aacataagtc ctgccatccc
     5941 atgttgctgg aggaaccaaa ggatatgctg aaccatccca ttgaacgatt ccataggctg
     6001 gaccgccaac ttgtgcaata tctggattca ttgcagcacc ggcttctcct tgaacatttc
     6061 caaggattcc agcgatggat gctttggaat accctctaga aagaagcatg acccataatt
     6121 cccatgcaaa tttgtctgct tgactggtaa cctctggtgg gtacatgcca ttccaactac
     6181 cgccatcatt atttccactt ccgccttgtc cagggaatac ttcttgtccg ttaattgtga
     6241 gttttccgcg aatattgacg tttttcataa attccgcatc accaaaggca tttagctttt
     6301 cctgtaggtt taaacttcca aataatatag aagttccatt cccaagtatt acccaacctt
     6361 ttccttcttt tggtgaaatg agaatatatt ttccatcgcc atttgttcga atgactaatg
     6421 aattatcttc taacggtgta ggagtggatg cgccaggaaa aggattgcct gcagaatcag
     6481 tagtaccaat tgtgccgata gattccttgg tgttccagaa ctccattcct ttcttggtta
     6541 gttccatgat ttttttatta tcattccaaa tttgtaacgt acctttgaca atttttagca
     6601 catcgcccag atcattaatt gagctttcaa gaacttcggc attaataatc ccaactttaa
     6661 tgaagttagc tacgatttca cccttcgacg tcattgcgat gtcaaacggg ccatttactc
     6721 cgttgtctga ataacctaag ccatttaaat tccaacgcca cacacgcttc gcattcgcta
     6781 cattgggagt atccataata agaatctctg atggagcttt ttctggacga aaaacgacat
     6841 gcccaccaga attaccagtg atccatgccg tcgcattcaa cacattttga actaacgttt
     6901 ctgttcgatt gtcgattttc tttttcaatt cttgtgcttg agtatttact gccgaagtgt
     6961 acagtgataa atcattacct aaaacaatat ccttatattt cccaagcgtt gggaaataag
     7021 tgtattcgac catacgctct tttatctcga tgtctagctc tttcgcccta acatgagcga
     7081 catcaccaaa gtgtaaagac gctagctctt gatacatctc accgtattcg agtgtgtgtt
     7141 ccaacgccac catgctgact gtatgagtag cttttggctc gtgaatacgg tcattgtcaa
     7201 ataaggtttt gccccatttg atcaattcat caactgtttt acaatcacca ttttcacgtt
     7261 tggcaatacg tcgattctca ttggtcactc catcaatttc taaataacca tattcgatag
     7321 gttctttgtc ttcatcataa tcgttatcag gaaccccgcc aatcaaatac aggctattta
     7381 cgatggattc ttcatctatt tcttcttcga ttgcttctaa attgatacca aaatcgattc
     7441 ggaaaccatt atctgatcca atctgtttta ccagtttcaa atcatagtta tccatatcaa
     7501 gttccgcgct agtcacgcca gtcaaatttt ggttaccgtt gtttgaacca atgattgctt
     7561 caactggcgc gacttgtctt gctgtaaatt ggtgcgttgt ccctacattc gaaagatagt
     7621 taaatttttg atcaaatgcc aaactgtttt taagattggt catgatctga gaaccgttgc
     7681 cgttatccgt gaatgactta acgataaagt ttttattggc cataaatcca atatgacgag
     7741 cagttacaga aacagacgtc aaattctttt tgatattgta aatttcgaag tattgccatg
     7801 atccatctgg caccttcgct tttatgaaat ttcctttttt taagtaagac cgatactgtc
     7861 cgcttcttga atagttacca taaaaacgat attggccatt caacacacga ttgatttctg
     7921 gcaaatcttc ccagtcaact aacgacgctc cgttcacgct caaatcatct ggcatttttt
     7981 tataagcata aataaattct tgcgtcacag atacacactc ctattccaaa accgcacttc
     8041 tttaaatttt ccggatattt ttacctgatt ccattcaggc tgcaatactg gccaatcacc
     8101 acgcgtaaat aaattcaaac cttcttgtat tgcttttcca agctgagtat caaccacgat
     8161 tgttgcagct aacgtattaa gtattgttag gcttttatcg ccgatagcaa tcgtaatatc
     8221 tcctccgttc gattctattt ccagataggg atgtgcaatt tcgtcaccat gatcaaaaat
     8281 atccataaca ctagaatgaa aaattttagg cgcttcaccg atttttcttt tgagtggctg
     8341 acaacgaaaa gtaacatcga atgtataaaa gaagccccac tcgttttcga atgggacttc
     8401 tttatccatg ctgcagatag catctagata cttgtctgga tcattatgag tgattaattg
     8461 gcttttacca gtaagccatc ttttgacttc cctcagtttg gaatgtggga tagtgatccc
     8521 ttctatttct aaatcaaaag gttcataatc atcaaacgtc tccgtcaatt ctccgcttcg
     8581 gccctgaacc gtgtaagttt cgtatcgctt gttcggcata atatctggaa gctctacctc
     8641 aatgatgcaa tccatatcaa ttactgcatt ccgatttttc caaataaaat ttggttcatc
     8701 tggattcata aattgtcgac tcaaatagga acacccccca aatcataaat agcctgttgg
     8761 tttgctttag caaatttacg attcatacga tctaactcag atggattgtt tgcatcgact
     8821 ttaccaatgt gtatatgctg ttcaatgcta cctccggaaa ctcttccgct gattcctttg
     8881 cgtttttctt cgtcagataa tggggtaact gttgtttttc cattttttgc agtcagtaac
     8941 tctggaccag cttcaccaac gattgcttga ccattgatta gatgaccgcc ttgtgctaaa
     9001 taaggcagtt ttgaaataga aaatgatttt ccacctacac caggaaccca ttttggtatc
     9061 tttatattat tcaatccacc aataaatcca ttgatcaatc caatcattcc attgaatggt
     9121 gcttttgcca tggctgcaat tccatcaaag ataccaccga aaatgtttac aacgccgttc
     9181 caagctcgtt cccaatcacc tgtaaatatg ccagttataa aatctataaa tccggtgaaa
     9241 attctcttgc ctgcatcgaa aaagttttgg aaattttgta tgattcctcc aaaaatattg
     9301 ctaatatatc cgcccataaa gttaaacact tctaccgcta tatcagaaac gccttggaaa
     9361 aaggcattca caccgttcct aaaccattca acattgttat aagcccaaac tagcccagcg
     9421 actaatgcgg ctattgctat tacagctacg gcaaaccatc cgccagataa tccgaataat
     9481 ttgcctagcc cactccacac agtggccaga tctttaaccc cagcggtgat cttagtaacg
     9541 gaactcataa gcgttcctat gacaaccaaa gctggtccga ttactgcagc tataccagcg
     9601 agtgtaataa tccagttctt agtaccttga tccagagaat cccaccaacc tttaaaactt
     9661 ttcaaagcat cgatcgccat ttcaaagaaa ggcaataagc ttatttggac ggattcgcct
     9721 acatcagcta aagctagttt cgcattgttc attgcttggt tagacttatc aattggatct
     9781 agcgtggctt caaatgtttc tccaaccgaa ccttgacttt catcggcgat ttttgccaag
     9841 tcttctaggt ttaacgttcc tctacgaatt gcatctgcca ttcttggacc acctttggta
     9901 ccaaaaactt ctgcagcagc gttgattgca tctgtttcag agcttgcatt tttgattttg
     9961 tcttgaagct cacccattcc ttgagaaaga gtcttcccat cttttgcgta agcaacagta
    10021 gctttcgata gactacttaa tgctgcacca gaatcaacac cagcttgttc gaattgcccc
    10081 attaaagtga caccttcgtt gaaacttagt cctaattgct tgatctgtgg cgcaccgtca
    10141 attgctttct gcattaaatc atcaactgat tgaccagtat tttgagaggt tttagttgta
    10201 acatccagta cagaatttaa atcgtcatat tccagaccgt atgcttcgat tgcttgtcga
    10261 gccattacgg ctgattgaga gacatcagtg tcgttaatct cagcatattg aaggagataa
    10321 tttgttgagt cttccaactt tttatccata aatccaaact gagtattcac ctcaccgatc
    10381 gcttcaccga ccgtttgtaa tggtaaatga gtgtttgctc ctactttttt gaacgactcc
    10441 cctaatgaat cagctacttt gccggtagcg ccagttttcg taataatcgt atctagggct
    10501 tcgtcgactt cgccgaatgc agcaagcgac gcagctccag ccgctacaat aggcgcggtt
    10561 actccaacag acattttttc accaacgcct tttactttct cacctgtttc ttcgattttc
    10621 tggagttttt ttgctgtatc aacagaaacg tcaccttgtt ctttaagtgc atcgttcgtc
    10681 tgttctaatg ctgtacgaag tttattttct ccggtttcag aattgagtaa ttgtttataa
    10741 agtttttctg attcagcaga atattcaccg gtttctttga cagatttttc atattcctct
    10801 ctcagcattt gagatcgttg ttcagccaat cctaattgtt tttctaattt ttgtttagcc
    10861 gctcgcaatt tttctgtttg agttgcatcc ttgtccatcg cagacacttg gtttttgtac
    10921 tcggcagcgg ctaagttcat ttctttgttg atttctttga ttgttttcga ataactgact
    10981 tcgccattcg ttttaaaatt aagaacgaca tcagattctt taccagccat ttatcttagc
    11041 gctcctttct accaccaagg cgatttatcc atagttacag attgaggtgg ttcaaaatcg
    11101 gtgttttgtt gtaaccactg taaataagat ttgagccaca agttaggtgt agacttcaaa
    11161 aagaaactct cactccaatt caatagagtg agagcaacgt aaatataaaa actccaagga
    11221 gttcctatct cttccgattc tttttgttta cctttctttt tgcttgcgtt ttttgaaagt
    11281 cttgtggctt cttggatttt tttaggtctt ccacctgaaa tgtctggcta gcaaaaattt
    11341 ccatacaggt actataagca gacaacacct ctccactcat tcccaaaaat ttgaaaatag
    11401 tttctggatc ttcctctaaa ccaccagtac gcagcatggc ataaattaag gcgcgcatga
    11461 tttttaggtc actaggcgat aagttagcag aagaaatttg tccttctttt ttagatagca
    11521 tggcattcat atcagattcg aattgcgaat aatctccacc atacacatct gctataaatt
    11581 ccatcgtttg catagtaaat gaaataggga attctgcgcc ttgaataatg acaacagcag
    11641 aattctttaa atcatcaaca tgaatcccat aatcggcaag tctggccatt atttgccacc
    11701 tccaacttta gccaatgttt cccactgttc ttcatcgtat acaggttgag caatgaattt
    11761 attaaataat tcgagagaag ctccgtctct attcgaatca aaacttgcat acatcacatt
    11821 attgtatttc agtccgatgg aaacaaaatt tgcagttaca tcgtcgattt ttgtctcttc
    11881 ttctgcagtg gcatactctt catcaattac attagatagt tgtgtgttag gataccatac
    11941 cgctttttta ccgccattct caatatttcc gatgaatcca aaggcaaaat acggaaattc
    12001 tcgtgcggta tttttgctaa acgttactcc acttttagca actaatcctt ttatttcatc
    12061 cattacttca atcggaatac ccacatgatc caacgcaatt tcatgttttg tttctcgcga
    12121 tacacgtcgg aacattttac ttgatgccca tttctctaac gctgtaccat tacctttgac
    12181 gccgattttt gtaccaatag gcaaacgaac aacttcacta taagttggcg cagtcccaac
    12241 ggtatcaggc gttgccatca tcgcaattaa aatgtcgtcg aggccttcaa aatataaagt
    12301 atcttgtttt cccaattaaa atcacccttc ccataaatca agtattttct ttgtcataat
    12361 ctcttctatt ttttctttgt tttgttcata tgtcccactt gcaaagtgtt gggctctttg
    12421 gttagttgct ccattttcaa caaatcgcca ataaaaagca gtttcttcaa actggacctt
    12481 cactcgatca tcatcgacca caacttttat ttgttcgctc atatgcttct ttttaagcag
    12541 ggacttagga atattaggaa gcaactgctc taggtaaaac tttgcagctt cttctaaaga
    12601 ttccaatgat aatttttttg gatcaactct agaaagattc cctaaataat ccgatatttc
    12661 agaaaaacca ttcttattac ttggcatttt ccatacacct cacatatgtg taatagttgg
    12721 ttacggtatc gtcgttctca tcaccttgta tacctgtaaa atctgaatat ggaataccag
    12781 cattttgtaa cgcttcttca ataacagcta aatcctgttc tgttccaagt gtgaaaaaag
    12841 aaatttgata atacggtaat tttttataga ctttaccgga tgccattttt ttactgttac
    12901 tcacatttga gtacacgatg tatggataca tcgtccctaa tctggcttta tctctgaaca
    12961 ctggcaactt tgttgatttc agcgctgttt tcaattcatc aaagctaatc gacataagct
    13021 aaactcaact ccatttctct tgtatcagga ttcgtataaa tgcgagtaat gttatacgtt
    13081 acagaatcaa ttttgagcgc acttaatttc tctgtgatgg atttatccca tctgacttta
    13141 attcgtctga caacgtctgt cttggcttgc tgtgataaat atttttcttg agaagtcaca
    13201 ccgagttctt catagaatat tagacgcttg gattcataaa ccgtagttgg acgatcgttc
    13261 tcatctgttc ctgttttgat ttctagcaat tcagctttcc atcgcagatt attggtctgt
    13321 cgtttcggca ttttgaatca ctccttgtac aataaatggc gtaatagcgc tcaaagcttt
    13381 gtctaactca tcttcagata ctcggtattc gtaggcaatg ccggcaacca tcaaaataag
    13441 atattcttgt tggccaccag ttgctgtttt gacataatct tttgccatat ttaaataaaa
    13501 agagagcaaa gaatcatcca tgccctcttc aaaatgaata tgtgatttga atttttcctc
    13561 taaagacaat tctttagttt gctcttccat cttaaccacc aactggtttt gtaatttcgt
    13621 agcgatatac tgccggttca aatggagaat aaaccaattg accatctagc aagttgtaaa
    13681 tttggaatcc aatttgattt ttaccagaga atttttcaac aagtttttga atttccaagg
    13741 caccaataac ttcttgaatt ttaaatgcag aaaaatcgcc aaaatataaa actggtgtgt
    13801 ctggtttacc ctttttatct gctgcatctg tccaatccac aggatagcca actaattggt
    13861 aacctattcc accttctgct tgtgtaaatg gacgcaacaa aggaaaacca tcatctgttt
    13921 tcattttttc aatagcagtc aaagcagctc gattaataat aaagcgtccc tttttcatca
    13981 cttctgtcac tggtgtattt ttaaattcga ttaatgcatc atataatttt tgcccagcac
    14041 ctgcagcagt tagatctaaa ggtttttcaa atgctacagc ctttttggct aatgcaccag
    14101 gattttcatt tccagcgtca tcaccattga acatataatt gatttcttta cgcacataag
    14161 cttttttcaa ttcttccaca acaatatctt caactggaac accagacatt tttagtaatt
    14221 ttttagttac tgttgccaaa gcatcgaatt cggcaggatc aagcaaaatt tcatcaaact
    14281 gaatagctgt ttcagcaata tcagttgaac gctctttctt gtttacattc gcatctgctt
    14341 tcttcacaag aattggatat ttgacatctc ctgatgttcg caccactgtt ccgtatttac
    14401 gaagtaaatt ttcttcttga gcataagtaa taacttcaga tgcaattact tctgggacag
    14461 taactgaacc gttgccagct tcaatcccta aagctcgagc ttctgcttca gaaatatttc
    14521 caactacaaa attagcaaat gcttttcgta gttgttgatc tttttgttta ttggtcattt
    14581 ttgcacgtgc ctccaatccg ttcttaattg atccaagtaa tccatctcgt tgctgttgag
    14641 taatcattcc agaacgattt tctgaactat cttcactgtc tgaatcttct tcttgatcgt
    14701 tgtcttcacc agaacggctt tcgtctgaat ccgtactatt cgattgatca tctgtattgt
    14761 cgtctgtttc atcagttcca gaatctgcgc ctaattcgtc tttaattccg ttcaattcat
    14821 caataacact gtcaatttct tctttcacgg cttctaaatc tgcttcacgt acttcaccag
    14881 attcgattcg tccgcgtaat tccgttaagc gttgctcatg acgagcttgt aattttttca
    14941 ataatttttt gttcatggtg tatcctccta tgcgttaagc gccccgttaa ttttttgaat
    15001 cattttttta cgtgttgcaa tgtcttgctg gacttcctgt ttgcttctag caagtgctgc
    15061 ttctgtatcc tgataggcag gaagagaaac gattgatact tcgaataatt ccacttcatt
    15121 caacgtacgc aaaacaggat cggtgttgta atcccacgtt tcttctgttg gaataaaacc
    15181 gaagctacat tgatcaatat ctccacgact catagattcg ataaggttat tagcatcggt
    15241 tgtgttgggt aactcaactt caaatttcaa cccacgttcg tcttcttcaa gccgaagtgt
    15301 tccacttttt gttcgtccta gaactttccc ccagtcatga tcaaatagac atcggacgtc
    15361 cgaatttgat aatgccttcg aaaatgcacc aggagaaatt acttcttcta agccttccca
    15421 aagtaacgtt cgactgttga acacagcagc atacccagtt acgattcgac tattattttc
    15481 tgtaacatct ctagtactca agttggtgat atcaaatgtc cgaatttcct tctttttcat
    15541 ttccatcacc ccctttcacg ttttgatcat tcgttggtaa ggaatcatct gttgcatttt
    15601 tctggccaat cctagacaag tcatttgaaa tatagatagc ttgtgtttct ggagtgttct
    15661 gtttaggaaa accaagcatt tctgccacat tatctggact tgtaatccca gtacgaacaa
    15721 tgttgtagcc aatatttgtt tttgttgagt aaggaacaaa atccaaaatg ttaattttcc
    15781 attccactcg atagccagaa ttaggcataa aaaaaagagc ggtgtaatgt tcgctcttgt
    15841 tcttcaatat tggtttgatt gctttgttgt gcagatacat catcgctttt tcgatgtctg
    15901 ttttcatcaa cgattgatac gtatcaacat tgattcctaa aaatttacct aagtcttttt
    15961 tataaacacc taaatagttc aaaatagccg cgtcatcaac aggacttttt aacgtctcga
    16021 tggaatatcc ttttcccaga ggaatcatct taacagaatg atcactgtca ttttgcgttc
    16081 cttccagttg atccaatata gctttgacaa ttttcgtttg ggcgctatta tttggattga
    16141 tgtgggcgtc cagtttaagc atgaacgcga gtaaaccacc tttagtatat ttgtccgtca
    16201 aaactttttc agcgctcaga acgccttcca gagtgtttct tgcaaggtca attattccag
    16261 caccttttaa tgaatcagtt ccgatgttct taatatgtcg aatcatttga ccaggtattt
    16321 tttgtccatt catttcaaat tcttcttgaa gtcgttcatt gattttagtg gttacaccgt
    16381 atgccaaatg aagctggtcc cgatctgtta atgggaatgt ctcaccattg atcagtaggg
    16441 tatttgtttc caatttggta aattcgaatc cggtcaaata atcattggga ttcttcaaaa
    16501 tttttagcaa gtggtggtcc ttcacttcat taccgtctgg acctatgaca acaggtgagg
    16561 acaacgctac ctggtttgag atatcctgga ccaattcata aacatcagaa gattccatga
    16621 tagaggaatc tgttacatat ctttgaccgt aacgcgtata gtggccaaac atatcctcga
    16681 tgtacccacg cttttccata aaggaataga ctgcattcga taaccgatca cgtaatttca
    16741 atatttctca ccgcctttct atttatctat agatggaact aaggtaatca tctaattcat
    16801 ccgaatcaat atctgtcatc tgattcatcg tttccttatg accacacaaa aacgccacga
    16861 aaccatcaat ctttttcttt gattgacgtt tacttggcgc tttttgtccg ttgatgttag
    16921 taattgctac aacattcaat gtgcaataaa ggaacaatgg attatcaaat tgaattcgtt
    16981 tctcataaaa caaccgttcg acatcatcaa aaggagcgtt caacacttta ggatattggg
    17041 caacctcaac gcattccaat cctaagttct ccaatttctc aacaagtttg tcgctcatcg
    17101 ctggatcata attcacttgt tgtatatcgt ataaatccat gcagtcttcg atgtactgca
    17161 agatttgatc ttgatcaatc attttaccat cgcaaaattc aacaaaacct tgttcagcta
    17221 aatcgctgta aggcacgtta tcttcttttt ctcgaaactc taaatcttca ttgggaataa
    17281 aataaagctg cttcacttta aggaccgctt ttccatcttc atcccacgtt gggaagttta
    17341 aagacacaca ggtcaaatct cgtgtgcgtg ataaatccaa accaatgtaa caaggttcac
    17401 cacttaaatt tccaagctct tgggtagtaa ccaaacacgg ctctacttga tcctgttcaa
    17461 agaaattatc cgcaccgttt acaaatacat ccaagtgctt cgttagaaac tcggcttttg
    17521 aatgtgctga acgttgtgcc gttttaaacg ctgactccaa ggcggaaaga tcaactgata
    17581 ttccccagtt aggattgcac atttcccaaa cttttctatc cgtccaatca tagtttttat
    17641 ttggttcata gattagaaca aagttcgaat cattatcatc acgtttcaac acttcttttg
    17701 cttcacgata tacgcgcatt ccaacagacg atgaaccttt cccagcagtt gaaatattaa
    17761 acatcaatgg ttgtggcaaa gagatttgtg cagacttaaa attgtcatac tgttccattt
    17821 tctcttgctt atgcaattca tcattcaata caaaatatgg attagaaccc tcaatgttat
    17881 cgatattctt cgtttgaaca ataaatttat tcgaataagc catatcttca tgaatataat
    17941 cataagtaat actagaaaca gttccctttg gtcctttgaa tattttagtt ccttcaagta
    18001 ggattggatt gtttaatatt gtagctgcaa aaggtttggc agcatattga gcttgagcaa
    18061 agtcggaagc gcacgcataa caatcgactg acaaagcacc ttctccatac attgcatagc
    18121 ctaatgaacc gacagcaatc aatgttttac cgttcttttt agggatttgg acatacgctt
    18181 cacgagtaac acggacaatt tgcccttttt cattttcttt aacccatcca tacatccacg
    18241 agtaaataaa tttttcccaa ggttctaaaa tgaatggctt tccaaccatc tcaccttttg
    18301 tgtgaacaat aaacgactca acccagtcca tcatttcatt cgcacgatct acatcaaacc
    18361 aaatatcttt acgttttttc caccgatacc aacgatccac tgccaaacga acagttttag
    18421 gatatttccc aggtttcttt cttacttctt ttgcaaataa atcggcataa tttacaccag
    18481 gttcgatcat ttttcagtac ctgccttctt acgccatttg tttctgtgcg ctgctagttc
    18541 atctactggc ttttcttctg gacgtgtaat ttcttcatct tttcttgcag tcgatccgcc
    18601 agttatttgt ctaccagttt tagccttatt cgttagcccc aacaaatcta gagctttcat
    18661 ttttttatct gcccaagttt ctacttgctg cgccaatgga tgcttcgatt gattagtggc
    18721 ccctgattta ttcgtgaatt tttgcgtctc cggaaagcct ttttccttcc acaaaagata
    18781 tttgtgttgg taaatttcaa aaatatccaa atatgattcg attaatggat caagagtgat
    18841 ggtgtacaaa tcagacaaat tcattatttt taaaatacga gctttttcag cacttacttt
    18901 ttcatcaaca atcgctttac gttgcgcttt agtcgtcata cttgtataca cccccctttg
    18961 ttttttgaaa aatttgacct aacgatgcgc gtgactcccc gctaccctat ctccccacgc
    19021 gaaaaaattt tgaaaatgga taggggggct tcaattgaaa tacgaaggga aaactttttt
    19081 gtcttccgtt tcattttcaa caattggatg acattttgaa cataaaagca tgagattgtt
    19141 tggatcaagc ttaagcagtt cattgtcttt gatgggtaca atgtgatgga catgtgccct
    19201 cttgccgaag ataaactgac cgcatcgctg acagtgacca ccttctcttt cataaataaa
    19261 ttgacgcata tccttccatg cctgtgttcg atagaatggt ttgttctcat gatgataaac
    19321 agactttgct tgttgcttct tcttacgtga tttgcttgat cgcttgtgtt cagcacaata
    19381 gatccccttt gctattttgt tcgtgcatcc gtcaaactga cagtacttca ttctgcttca
    19441 cgaataagat ttatgatatc gccttttgca cggacagcac caggaatatc aatgccatgt
    19501 ttcttagcat atgcacgtaa ttcttttgca gtcatattgt ccagttcatc cgtatcttca
    19561 gtggactgat cattagtagc ttcgtctcca tcaaaatcag cagcagtgtt tccatcgcta
    19621 tcaagaattt cactgctatt agtaataagt tcaccattaa cagccacaaa cgttttacca
    19681 tcacttaata ttcctttttc ttccgttgct tcaaaatcag gttcttgacc tttcggaact
    19741 acgacagtct ttttcttttc tgaatcccaa tactctgttc ctgtgatggt tgttctaatt
    19801 ttgatcattg ccattttgat tctctccttt gtaatttgtg ctaattactt ttgcacccat
    19861 tcgttcatac cattcaacag tttcctgcaa attgggtaaa gtgtgggata ataaagaaat
    19921 aaccaacaca tccattgcta tcccacgatt atctttatct acttgcacat ttgcataggt
    19981 gccgttccaa ttgtttcgta aaccagtagg actgttctct gcttctattg ctttcttata
    20041 gacatctccc atattcgttg ggacattcgc cataagcatt gcctcatgaa aatcattcat
    20101 agataaaact cctttcaaaa taaaaagacc actcaaagag tgatctaata tgtaatgcac
    20161 aggcagggac gtttccgatc ctgtgcttga gtcatttgac gattcatttg taccgaatcc
    20221 caaaactcaa tctaacctaa ccaattatgt aatagcaacc tacaccgatt ccatcgatta
    20281 ctatcgacct cgccatgctc gtgtactttg agcgcccatt tccaaccctc ggttgctaac
    20341 gaatactgaa acagaatacc gtctctgttc atgaccaacg cgacagtaca aagctttgtc
    20401 gcaagcaggt tatcgtattc ctaataaccc caactaatgt ttctctccta acctacacct
    20461 gagagtggcg cacgtctgcg ctagtatttt atgccttcta actgctagta gcatcccgac
    20521 tagtcagcca attcatcggc atccaataag atgaattgtt tccgtaggtt ccttaagcca
    20581 ctggcaagga atcgaacctt gcatggatta ctctcattca tcctttattg cgtcttctac
    20641 ttccgccaca gcgacaaatt aatattgtga aaataaatac taagcgtata attttagtta
    20701 tcagcgagtg gtccgctgaa ataaatatac gaaggtgaaa actatgttaa ccaatgaaca
    20761 acgcgctcat gatttagcaa ttgcaacatt accttttgtc agagatattg ttaaatctca
    20821 aataattgaa gggaaagatg caaaatttga tgcatatttt gaatatataa agctttataa
    20881 tcaatttctt tccgctgttt ctgaagattt taaaaactag gagcaaaagc ttgaccgtta
    20941 attaaaaaat ctctactttc tttataaata
//
LOCUS       DAHEYX010000028        20691 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHEYX010000028 DAHEYX010000000
VERSION     DAHEYX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.028"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            352..1041
                     /locus_tag="MB219_000718"
     CDS             352..1041
                     /locus_tag="MB219_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426453.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="MB219_000719"
     CDS             1028..2236
                     /locus_tag="MB219_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCC8426454.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="MB219_000720"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="MB219_000720"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCC8426455.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="MB219_000721"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="MB219_000721"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCC8426456.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="MB219_000722"
     CDS             complement(6339..7034)
                     /locus_tag="MB219_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCC8426457.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="MB219_000723"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="MB219_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCC8426458.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="MB219_000724"
     CDS             complement(8233..8424)
                     /locus_tag="MB219_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCC8426459.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="MB219_000725"
     CDS             complement(8445..9335)
                     /locus_tag="MB219_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCC8426460.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="MB219_000726"
     CDS             complement(9322..10089)
                     /locus_tag="MB219_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCC8426461.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="MB219_000727"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="MB219_000727"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCC8426462.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="MB219_000728"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="MB219_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCC8426463.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="MB219_000729"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="MB219_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCC8426464.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="MB219_000730"
     CDS             14660..15043
                     /locus_tag="MB219_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426465.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="MB219_000731"
     CDS             complement(15211..15969)
                     /locus_tag="MB219_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCC8426466.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="MB219_000732"
     CDS             complement(15987..16805)
                     /locus_tag="MB219_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCC8426467.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="MB219_000733"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="MB219_000733"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCC8426468.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="MB219_000734"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="MB219_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCC8426469.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="MB219_000735"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="MB219_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCC8426470.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="MB219_000736"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="MB219_000736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCC8426471.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5927 a         4219 c         3799 g         6746 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgttttc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct g
//
LOCUS       DAHEYX010000029        20620 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHEYX010000029 DAHEYX010000000
VERSION     DAHEYX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.029"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            53..1393
                     /locus_tag="MB219_000737"
     CDS             53..1393
                     /locus_tag="MB219_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8426472.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1611..2054
                     /locus_tag="MB219_000738"
     CDS             1611..2054
                     /locus_tag="MB219_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCC8426473.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2558..3976)
                     /gene="nhaC"
                     /locus_tag="MB219_000739"
     CDS             complement(2558..3976)
                     /gene="nhaC"
                     /locus_tag="MB219_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCC8426474.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4379..5602)
                     /locus_tag="MB219_000740"
     CDS             complement(4379..5602)
                     /locus_tag="MB219_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCC8426475.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5633..6472)
                     /locus_tag="MB219_000741"
     CDS             complement(5633..6472)
                     /locus_tag="MB219_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCC8426476.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6489..7169)
                     /locus_tag="MB219_000742"
     CDS             complement(6489..7169)
                     /locus_tag="MB219_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426477.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7171..8244)
                     /locus_tag="MB219_000743"
     CDS             complement(7171..8244)
                     /locus_tag="MB219_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8426478.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8300..8538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8581..8814)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8891..9562)
                     /locus_tag="MB219_000744"
     CDS             complement(8891..9562)
                     /locus_tag="MB219_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCC8426479.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9649..12327)
                     /gene="mgtA"
                     /locus_tag="MB219_000745"
     CDS             complement(9649..12327)
                     /gene="mgtA"
                     /locus_tag="MB219_000745"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCC8426480.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12986..13423
                     /gene="ndk"
                     /locus_tag="MB219_000746"
     CDS             12986..13423
                     /gene="ndk"
                     /locus_tag="MB219_000746"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCC8426481.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13445..14311)
                     /locus_tag="MB219_000747"
     CDS             complement(13445..14311)
                     /locus_tag="MB219_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCC8426482.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14471..16564)
                     /locus_tag="MB219_000748"
     CDS             complement(14471..16564)
                     /locus_tag="MB219_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCC8426483.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17313..17936)
                     /locus_tag="MB219_000749"
     CDS             complement(17313..17936)
                     /locus_tag="MB219_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426484.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18095..19492)
                     /locus_tag="MB219_000750"
     CDS             complement(18095..19492)
                     /locus_tag="MB219_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCC8426485.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19524..20495)
                     /locus_tag="MB219_000751"
     CDS             complement(19524..20495)
                     /locus_tag="MB219_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8426486.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT         5874 a         4310 c         3588 g         6848 t
ORIGIN      
        1 atttattctt aatataaatt ttgattgtga atttaaaaga aagaggtcat ttatggaaga
       61 taaaaaattg aaaaaacaac ttagctccag acatatcacc atgttagctt taggtggagc
      121 aatcggtgca ggtctattta aaggaagtgg cgaagcgatt gggatcgccg gcccttctgt
      181 attgattgct tttttgattg gtgggctgat tttatatatc gtgatgaaag gtctgggaaa
      241 aatcgtttta agcggtggcg acacgcatca cggattatct ggtttgatac gcccttatct
      301 aggtgctcat tctgctgatt ttactgattg ggtctactgg tctatgtgga ttatcaatat
      361 catcgctgaa gctgttgcag ctgcttcctt tttgcagttg tggttcccgc acattccagc
      421 ctgggtattt gtttttatgt tggctgtttt aactacgatc atcaatcttt attcggtacg
      481 gttattcgca gaaacagaat attggctggc ttttgctaaa atcagtgtca ttatcttact
      541 gatcattttt gctacttatc ttgtcggtca gcaaatgttg ggcacaggcg ttttccctac
      601 ccttcagcaa ttgactgacc atggtgggtt tactcctcat ggtatgaagg gaatcatcag
      661 ttctttgctt gtagttatct attcttacgg cggttctgaa ctgattgcta tcacggtaag
      721 cgaagcagat gatcctaaac gtgcgattcc taaagcaatc aaaggtgtca tgggaagaat
      781 catttccttt tacatcattc ctttgttttt gcttttgatt ctttttcctt ggaatacttt
      841 agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa aaaatgaata tcccatttgc
      901 agcggatatc gtcaattttg tgattgtcct tgctttgttc tcatctatca attcgggtgt
      961 gtatgcatct tcaagaattt tgttcttccg tttgaaggat cgaaaaggtt cctctagaaa
     1021 actagctgtt ttaaacaagc atcaagtacc gcaacgtgct gttttatttt gtaccagtgt
     1081 tctttatctt ggtgtagctc tttcttattt tgtcggtgac aaattatttg gctatctggc
     1141 aggttccctt tcgtatacag tattattgat ctggatcatt atcagtgcgg catcctttgt
     1201 actggcacta agaaaaggaa cattatggga taaaggaatc agcttttttg cattagctgt
     1261 cttaggattg attttccttg gtatcctgtt cacgaactcc attggggtaa ctgtattgac
     1321 tggcctgctt tacctgttca tttatttcag ttatcaaaaa aagaatgatg cgttcgtatt
     1381 gacgaatgaa tgaaatcgat agaaaatttt attttattta tgtggaatag atggagctat
     1441 tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct ttttccgaat aaacagtctc
     1501 atgacctctt ggtaaacggt gttcaaaaac cctctgtgca aaataattga ttgttctcca
     1561 tgctcatgac atgatagaag aaaggaatga aaaaaaggag tttgttttaa atgaaaaagt
     1621 attggaagtt tttgttgatg atcggtgttt caacggtagt tatgtttggt atgatgtact
     1681 tgaatgtcta tgcgctcgat catatcttct tcagtcgtac acgagtattc atggcgttga
     1741 tgatgggtgc agtcatggca gtcatcatgc tgttgttcat gtggaaaatg tatgaaaaca
     1801 aaaagataaa tgttatgatc ttagccgcta gtcttcttgt ttttgccggt tccttgttta
     1861 tggtacgaag ccaaactact gtcaacgaca cagcttggat gaaggccatg attcctcatc
     1921 attctatcgc tatcctggca agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat
     1981 tagcggaaga aatcattcag gcacaagaag aagagatcga caaaatgaaa aaaatgatca
     2041 aagaaatgga atgaccatta tccgtccaaa taaaaaaaga ggatgttcca gaagttatca
     2101 gcttcaagaa ataagaagaa gctacttctg gaacgccgtg aataaggggt tgtgaaaaag
     2161 ctgccctgca tcaagtaata agaacagcca aacaaaaata gcttctccta tttttcgttt
     2221 ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattaaga ggttgtaaaa
     2281 tcagcgttct gacctgagta ttagagagac aaatggaaaa atagttcctc atattttgcc
     2341 attttttgag ctaataccga gggtcagctg attgaacacc gtttatcgag aggttgtgaa
     2401 aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa atggctttcc acatttttgt
     2461 ataaatttgg cttaataccg agaaactggc ttttgaatac cgtttattcg taaaagggat
     2521 tgtgacaaaa gtcttgtcac aatccctttt tatgttttta ttgttctaat tgcgctgtct
     2581 tgcttactgc ttcttgcggt ttcacaaatt tttttgagat tccccaacct gtgaaggcac
     2641 tgaccatcga aaacacagga gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg
     2701 taggaatacc aaatgtactt gctacgaaac ttccggcaat tccccaaggc actaagtagt
     2761 tgattactgt tccaccatct tctagcgttc tacctaatac agttggatca aatcctgctt
     2821 tttcataagt ttctttaaat gcattaccag gcagaataac agataggaat tgttcaccaa
     2881 taaaaatatt tactccgata cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg
     2941 tcatctttct tgatactcgt tccatcacgg ttgagatcaa gccaaattcg atcaacaatc
     3001 cacctaatga caaagttaga ataatcaatg acacagtagg catcatactt tcgattcctc
     3061 cgcggctaag cagtgaatcg atttgttggt ttccagtatt tgaaacaaac cctgattcga
     3121 tgaccccagc caacttcgtt aaggaagttt gtgggttttg aataaaaatc attcctactg
     3181 ctacagcgat attcaacaaa atcgttaaaa ttgccggcac ctttttccat gcacacagta
     3241 acattaaaaa taaaggaatc agtgaccaga ttgaaatagt aaagtttgct tctagaacat
     3301 cagtcacttc acgaatagat gaaagacttg cggctttact tgtgtgtcct aaaatcatga
     3361 ataaaaagaa agaaacaaca aatgccggga cagttgacca catcatatgc ttgatgtgtt
     3421 tgaacaaatc ggcatcaaca atagctgctg ctagatttgt tgattcggat aatggagaca
     3481 tcttatctcc aaaaacggca cctgaaacaa tagctccaac aaccagcgct ggatttaagc
     3541 ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac
     3601 tccctacagc gctacctacg attgcacaca caacaaatac agaagggaca aaccactgga
     3661 cactgatcaa tttgaaccca atgaccatta gagttggaat gatccctgct tgtatccata
     3721 cagcaatcaa tgaaccgact aaaataaaaa taaagattgg gatgattcct ggtttgatgc
     3781 cattgatgat tccttcgtgc agccaatctg tcgggtatct ttttatcact gcatacaaca
     3841 tgaccattcc gatcgctgct aagatcgtga tattaggtga gacctttaat ccgacaacgc
     3901 caatcgcaat gcctaaaaca ataaccatta atacaagtgt tgcttctaaa aaacttggtg
     3961 ttcctttttt tcccatgata atctctcctt ttttagttgt tcttatgttc taaaaaagtt
     4021 aagatatcgg attactccgc cgcaattcat ccgcgattcc tcctttataa aaataaaaag
     4081 acctgtttta aactaaggac gaatgactcc gcggtaccac cttaattaaa aacagatctg
     4141 tttttcactt tcaaactttt attcaaatgt atggactttc catgtaattc gtcaattagg
     4201 cttgatcggt tcacagcgac caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg
     4261 aaaagttgac ttgtacgaag tttaccttcc ataagatcat ttgtcaatga aaaagaaaat
     4321 attttttcat ttttctgaaa attattttta taactttcac acacaagttt attttttact
     4381 agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct tgcttgattc
     4441 tttctaatag ctttggctct ttcaacagat caagagctgt attggcgagt agttctgctg
     4501 caatcgcaat agaagcaagg cctttgtcac ttttagcagc agctttgaat tcttctgtgt
     4561 gtccggcaat atatgtctca gagattgcta ccgtaggctg gattgttggt atgacttggc
     4621 tgacatttcc tacatcagaa gaaccgagac ttgttctttg ttccgtttct atttcttcat
     4681 ctggaacacc tgcttctttt gcatgctgga agaataagtc atcaaaagaa ggagtaacga
     4741 tgacatcatc aactgcattt tggaacaatc caaactcaaa agtcgtgccc gtacttatcg
     4801 cagcacctcg tacgatattt tccacttttt catatacttt atctaatgtt tgccgatttc
     4861 ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat gacgttggca gctactcctc
     4921 cgtctgtaat gatcccatgg atattgacat cttttggcaa ttgcaagcgt agagcgttga
     4981 ttccattaaa cacttggatc aatgcttcca aagcatttac tcctttttca ggtgctgctg
     5041 ctgcatggga agcgacaccg taaaatttga tgtcgactgg atcattcgct aaagattctg
     5101 tcgttttccc ataacgaaaa gctggatgga cgcataacgc tacatcgaca tcctcaaaaa
     5161 aaccttctcg aacaaagctg cctttagctg atccgttctc gcccccttct tcccctgggg
     5221 tgccatatac acggatttct ccaccagttt catcaatgaa ttgcttcact atgctggcgg
     5281 ctaatacgga gtaagtacca aacaaattat gcccgcatgc atgaccaata cctggtaaag
     5341 catcgaattc tgccaaaaag gcaattgtgg gaccgggttt attggaaata taacgtgcat
     5401 caaaggctgt tcggtgtcca gctacttctt tttctaccca aaacctttct ttttctaatt
     5461 gtttaatcaa cgctgctgag gaataaaact catagttact tacttcagga tgttcgtgga
     5521 tatctagagc aagtgcttga tattctggta aacgatattg gattagatcc ttcaattggt
     5581 ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt tcttattttc
     5641 tcccgaatgt ttcccatgca ggcacgcttg atccttttga tgttttctcg attactttga
     5701 ctgtgtcttc tgtttgataa gcgtctacta ttttttgata cgttttattg ttttcatctt
     5761 cttttcttgc aacgatgata ttcacataag gacgtgcatg gtcatccact ggttctaaga
     5821 agatggcatc agaagcagga ttaaatccag catctacagc tacaccgcta ttgatcactg
     5881 aaatgtctac atctgacaaa gcacgtgctg tttgggaagc atctaattca gaaatgtcta
     5941 gattttttct attctcggta atatcagaaa cagtgggcgc ttgtcctttt gccttatcca
     6001 ctttgatcaa tccagcagtt tgcaataaca gaagagctct gccgccattt gtaacatcat
     6061 tagggattgc tacttttgct ccgtctttga tatcagctac atccttcaaa gtgttagaat
     6121 aaattcccag cggtgcattg acagtatttc cgatcgatac taaatcagtt ccgtgttctt
     6181 tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc gatccatctg
     6241 ctaaagccac atttggttga ttgtaatctg taaatttcac gtattccaaa ttaatgttct
     6301 cctttttcaa acggtctttc acgttgtccc atacatctgt atcttctcca ataatcccta
     6361 gttttacgat agtttctttt ttgtcagttg tatctgcttt tccttggcta caccctgcca
     6421 accaaataat tgctacaaat gtaatgacta caaaacgaat aatttttttc attttttctc
     6481 ctccactttc aatgtgtaat ttttttaatc agttgattac tgataaattg acttaaaaaa
     6541 actaagatta gaatcaataa tgtagccaca atcgttacat ctgtttggaa tcgattatac
     6601 cctctcgata tagcaagatt ccctaatccg cctgcaccta ctgctccagc catggtagtc
     6661 agtccgatca cgttgatgat agtcaacgcc gagacccgaa tgataccagg caaaccttct
     6721 aacaaataca cacgtaaaat gataccgatt ggactggttc ccatcgcttc tgccgcttcg
     6781 atcaccccag gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca
     6841 actccaaaaa tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa
     6901 cgagtagtca atgctaaaag agaaagcaag ataatgaaag ggatggaacg aatgatattg
     6961 atcagcttat caagtatttc aaatagcaga cggttagcta gtaatccatt agggcgtgtc
     7021 actactaacg tcacacctaa gatgattcca atcatcccgc caatgaatgc tgtccaaaaa
     7081 gacatatata gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa ataaacattg
     7141 ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct ccttcgatca ctttgatacg
     7201 actatctaaa tcgttttttt gtaaaatatt tatttttaca ccagatttga tcaaataatc
     7261 caatgctttt ttccgctgtt tggtttctcc agctaaagta acgatcaaac tccctaacgg
     7321 cgtttcttgt aataactcta cattgccata taaaatattc gctgttactt gatatttgct
     7381 gtacagatga gcgattagag gttcgttcgt ctgatttcca atataactta attccaccag
     7441 ccatttattg gcgatctgat cggcaaaagc agaatgggca atgatcgttt ctaatgcttg
     7501 gtcaagatga gtagctgttc gaataaaatc tttggtcaat tcttcttcag gattgctgaa
     7561 gatctggatg ctgctacctt tttcaacgat tcttccatct tccataaccg ctaccttgtt
     7621 gcagatttct ttgacaactt gcatctcatg agtgatcagg acgattgtta aacctaactg
     7681 gcgattcaat ttttttaaca atgcaaggat ctgaagggtc gttttcggat caagtgcact
     7741 ggttgcttca tcacataata ataccttagg ttcattcgcc aaagctcgtg cgattgctac
     7801 tcgttgtttt tgtccccccg acagttgttt aggataagca gatgcttttt cttccaaacc
     7861 gacaagttcc agtagttcat tcactttttg acgacgttcc tgtttcgatt ttccagaata
     7921 ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt tccattagat tgaaatgctg
     7981 aaagatcata ccgatggcct ttctttcttt tcgcaattct ttgggagaga gctgactcag
     8041 attcgtctga ttgacaatga cttctcctgc tgttggtttc tgcagcaaat tcatcacacg
     8101 aactaacgta cttttccctg cacctgagta accgacgatt ccataaacat cgcctttttc
     8161 aatcgttaaa tctacagaat ctactgcctg tatctctttt tctttttgtt tgaaacatac
     8221 agaaacatct cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag
     8281 aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt gttaccacct catgttcaag
     8341 ctgtgttcac acacaactcc tcttcaagta cgatcatact ctagcaagat atcggtcgca
     8401 gccgtcatcg ccttacatct atgctcggga tgaattgctc gatggccatt ttccaatagt
     8461 cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct atcgtactta
     8521 ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt actaacataa actatgtcag
     8581 taaaaggacg acgtatcgcg gtaccacctt ttttcggaag tttccttccc tcaagcccaa
     8641 catctcaaaa agagagattg gcgttcgttt taacggtcga acagttccga atttgcctac
     8701 ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact cttctcacca
     8761 gccagagcct ctctgcaaag tttccaaact tactttcctt ttcaacgaaa tcgatatttt
     8821 gttgttggga ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt
     8881 ttttctatat ttatttttct gtttttttat ttaacatttc aataacagtt cgaagatctt
     8941 ttccttccgc aagaagtttc aatttttcct tttcttcgac ggtataattt cggacaaaac
     9001 tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt gctcctacta
     9061 aagaatcttc cactgcaaag gctgaactag cttctaatcc tgttcgttcc aaagctgtca
     9121 gataaggaat cggtgaaggc ttgttctccg acacttcgtc tccgaatgta aacgtgtcca
     9181 agtaatcagc taatttaaag tgatccaaga tatctgttgc tcttttcgta acagtggtcg
     9241 tagcaatgcc tagtttcata tgacgttctt ttaagtctgc caatatttct tttgcatatg
     9301 gttttaagtg gatggctccg cttgtcagct gttgataaaa atattcttca cgcttcgctc
     9361 ggacttcctg tactttttca gggctacctg ctgctttcac taaaatatca aaactttgtt
     9421 tccagttttt tccgccccaa gcgtcaacga tttcttttgg gatatggata tcaaattgct
     9481 gaaatcccca aagccagcca tcacgatacg ttttttctgt gtcaactaac aaaccatcta
     9541 aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata cacatagttt
     9601 agcacgactg gtccaaaaag aacagaagca aagaaccagc cttctcgttt ataagagttc
     9661 ttggaatcga cggatataga ttttcttcac agctgtcaca agcgccagat aagcgatgac
     9721 tgtagcaaat aaccataacc agtaattgcc aggaagtggc atcaagccta attcagcacc
     9781 aaaacttgta aaaggaagga cagaaccgac aataattcct aatgtagtga taacaaacat
     9841 ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt gtacgcagtg catggagcac
     9901 taaggtttgt gtccatagtg attcgacaaa ccatcctgca tggaataatg cgatgaaaat
     9961 cactcgctgt gaactatcca atgtatggaa acttccgccc actacagcag gacaaataac
    10021 aaaatacatc aacaagtaag tagtaatatc aaatatcgaa cttgttggac caaagtaaac
    10081 catgaattcc ccaatactag atgcattcca tttttttggc tcgtccaaat attctttatc
    10141 catccgatcc catggcaagg aaatgcagga aacatcatag atcagattca aaaacaaaag
    10201 ttgtaaaggc agcattggta aaaatggcag gaacgtactt gcaatcagca cggaaaacat
    10261 attgccaaaa ttagaacttg ctgtagcctt gatatatttc atgatattcc caaaagtttc
    10321 tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc
    10381 tgcagattct ttcgcaatat ccacagctgt atctacagaa ataccgatat cagccacttt
    10441 cattgcagga gcgtcattga taccatctcc taaaaatccg acagtatgac catttttacg
    10501 taaagctgtc gtcaaacgtg ctttttgctg aggattcaat tttacgaata cttcatggtc
    10561 ttcagctact tgtgctaatt catgatcact catttccgag attttctcac cggtaaccaa
    10621 ttcattgatc tctagaccta cttgacggca cactgaacgt gtaactaatt cgttgtcccc
    10681 agtcagaact tttacagcta caccatgttc tttcagagct ttcaacgctt gtttcgttgt
    10741 ttcttttggt ggatcaagga aagcaagata accaatcaaa accatgtctg actcatcttt
    10801 gactgaaaat tctccaacga cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa
    10861 gccatcttca ttaagtgact gtacttttgc aagtacggct tcttttcgtt cttttgttaa
    10921 tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg ttcagcattt cttcaatcgc
    10981 gccttttgtg atcatttgtg ttttaccagc tggatcagca accacaacac tcatacgacg
    11041 acgttcaaaa tcaaaaggaa tttcatccac tttttgataa ttgatcttat ctgtatctaa
    11101 agtttctgtt gcttcatcaa tgatagccac atctaacaaa tttttcagcc ctgtttgata
    11161 gtagctattt agatacgcat ggcgcaaaac acgatcatct tcatttcctt cacaatccat
    11221 atgatattca agaatgattt tatcttgtgt caatgtcccc gttttatccg tacataacac
    11281 atcgattgca ccaaagttct ggatggcatt caaatttttg atcactgttc cttttttggc
    11341 catcacactt gctcctttga ccaaattcgt cgtaacgatc attggaagca tttcaggtgt
    11401 tagaccaact gctacagata gtccaaaaag aaaagcttct agccaatccc cttttgtaag
    11461 cccattgatg acgataactg ctggagccat caatgacata aatttgatca gtagcatgga
    11521 tgttttatgg atgcctaatt caaagcttga ttgaattggt ttttcagata gcgtttctgc
    11581 tacatgacca aatagtgttt catttccaac agaaacaatg attccttccg cacttccact
    11641 gatgacatta cttcccataa agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc
    11701 ttcacatact ttcgcatgct tttctactgg atagctttcc cctgtcaatg ctgcttggga
    11761 aatgaataaa tcctttgcgg acaataaacg catatccgct ggtatcatat cccctgctga
    11821 aagccgaaca agatctccac agacgatttc ttccatagga atctctgtgt actttccatt
    11881 acgttttaca gctgccgtta ctttcacaag tgatttcagt ttttcagccg cttgattgga
    11941 acgtacggac tgaatcaacg tcattgtccc gctgacgata accatagtga agataatggc
    12001 tacaccgaaa agatctttat ctccaggagc tgctaataca tattccgtga tgaatgaaat
    12061 aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca atatttctac
    12121 aatgaatggt gtttttttgc catatgaaat cgcattttca ccgtaatttt ctcttgaacg
    12181 ttcgatttgt tcttctgtca atccatttgc tgatgcatgg aatagttcaa ataattcttg
    12241 tttcgttttt ccagcaaaag ctgcatagat tttttgtgta tctttctttt tttccatctc
    12301 aatctctcct ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt tagctattca
    12361 tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag atagataata gccctggcct
    12421 ttcctctaga tccttgtttt tcgaatgact gtcatccatt tgttctcatc tcctttacta
    12481 agtaatttta attcatgact tccaatcatt tttcgaatag ttatttttcc aattagacca
    12541 cctccttttt atttttcacc gatggggatt tgcggcaaca aaaaaacctc tcgccaaata
    12601 ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc tcagatctca tcgttgagtt
    12661 ttgacactgt acaacgtaaa aagcagatac ctgcttttag ctaccttatg aaaagcctta
    12721 tctcgacaat tcctgttcta cccattggcg tctctcgacg tttttgggca gtagcctatg
    12781 tttgtatagg agcctcacct aacaaatctt attcggtttt ccttcgttaa tataacaact
    12841 aaaaaagagg atgtcaaatc aattttccat gtttttcata ctttttttaa aatacacacg
    12901 ttatattcct gtctcacgcg gttttatttg tctcattttc cacctcccgc tataatagaa
    12961 atatagtagg aaacgaggtg ctgttatgga aagaacactt gttattatca agccagacgg
    13021 cgtgagacgc catttagtcg gaagcatcat ccaacgtttt gaagcaaagg gactggccat
    13081 tgctgaaatg aagtttgaaa ctatgactcc tgaattagca aaagaacatt atcagcactt
    13141 gactgagcgc agtttttttg atgaactgat cgattacatg acttccggtc ctgtcgttta
    13201 tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt aagatggttg gagcaacaaa
    13261 agctgccgat gctgttccag gtacgatccg aggagactac gcgctgccgg ggactgaaaa
    13321 catcattcac gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc
    13381 gccattcgat ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc
    13441 tggtctagtt gctgggttca gcaatggcca gcagtttttt ctctaaggac ctccgttgat
    13501 tctttcctaa catatattcg agcactgtga agaattcttg gtagtctttc gtcttttctt
    13561 cccctaaagc agcagaaatc aatctttgat aataatctgc catcaatgtt tcatagatca
    13621 ctgtacttct ttctttatgt gctaagctcc gtttcatatc ttcattcaca ctacgtgcct
    13681 ttttcaaccg accattttct atataaaacg aagtcaggtt cattaaaaaa gtaaagagat
    13741 actgctcatg attgttcaac tgtgctaatc gtcgcattct ttttttgatt ttcaaataat
    13801 tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc agctagaata gatagttcaa
    13861 attgaccata gtctggtatc tgcatcaagt attcttcaat gattcggcat tcataacgta
    13921 aaaattcaaa ttctgtctca gatacttctg ttttttcgac catgtgaaga ttcaaaacca
    13981 aataatacag atcttctgtt ttcttaaaat gcctacggtt attgctgatc tctttataag
    14041 gatcaaagct gtcttcttgt acccgatcta gcattcgtct ttttttcttt aactttattg
    14101 gttcatccag ctggtttcgc atccattcat attctgcaag tgtcatgttc attcgctcta
    14161 aaaatgatct caatgttaca aatgacattt ttttcactga atttgtctca atcccgacta
    14221 gagaagtctt aaaacagatt ccttccgcta attgggactg tgtcaacttc ttgttgactc
    14281 gaagtttctt gatcaattca ccatcataca taatagttct cctttttaat ttaaattttt
    14341 ccacattcaa taaatagata cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt
    14401 ttttttcata aaaaatacca accttcaagc attagaatgt tgtctaacta ctgaaggttg
    14461 gtttgattgt ttattgatta tttttttgct gttcaacgtc tgttcttgtt actggatata
    14521 agttggttgt tgttttatta ccattttttg aaaagacact tgtcgaatgt tcgccttttt
    14581 gcttttcgat tgcttcatta cgttccgctt catttttata gtcatatttt ttcggatcaa
    14641 tcgtctcaaa tccgtttggc acataaaacc gcaagagatt ttcctgattc agcttatctg
    14701 aaagacttaa cgcagtcttg actttttcct gatctttgga gatttcctca tcgattcctg
    14761 ctgctttcgt atcgatgatt tctcctgtct gattattgta agcttttcct cctaggacgg
    14821 tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac tacttgatca tgctgagaag
    14881 ataataagtc tgtaccaaaa tgaagataat ctttatcatc gattcccaat agatgtagta
    14941 atgtcggcaa aacatcgatt tctccaccgt attcatgatt gactgtccct ttagtataac
    15001 caggcatatg gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg
    15061 tatctggatc tttaccaaga gcgcttgcta aatctttatt atctgtattc gagatcccaa
    15121 aatgatcccc gtaaatcaca aagattgaat tttggtaaat accagatttt ttcaaatacg
    15181 taaaaaattg ttccaatgat tgatccagat aatgtgccgt tcgaacataa ttatctactg
    15241 tgctattgcc agtattcaaa gatggaaagt cgaaattctt gtcatccgta taataaggcg
    15301 tgtggttggt tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt
    15361 gggcagattc tctgaataaa tctttatcta atatcccata acctaacgtt tcgtcggatt
    15421 catcaaaata agatcgatca aagaaattgt cataacctaa gtttttatat acatgatctc
    15481 tattccaaaa actagctaca ttcccatgga aaacagcgct tgtatagcct tgcttttgtc
    15541 caaggatcgc tggtgcggct tgaaagacat tgtctgaccc aagttctgtg aataatgagc
    15601 cttgaggtaa accaaatgtg ccagtttcca acatattttc agcatcactt gtctttccct
    15661 gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat
    15721 ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac
    15781 tttctaaatg gagaacgatc acatttttcc ctttagcgat cccaaatgtt tcgggatttg
    15841 gctctgcata atgtttcttt gtataatcta aaacttcatc aatcccatta ctgcttgcat
    15901 gcgcgcgaac actgctagtc attcctgttt tgattccgtc ataaatagta tacgcatcga
    15961 ttcctagata tttgacaata tatgaacggt caaatgttcg ctgcagcaat tgtggacggt
    16021 tggcttcgct cagcgctaga tttcctgaaa aaataagccc tgaaagacag gtaacagcga
    16081 tagccatcgg tttgccgaca gggttgcttt tgataggtat tttcttgacc ttcaaccaaa
    16141 ccagtagccc aatgaacaca gcgatatcca gccagtagat gacatcatgg ggcttcatca
    16201 aagaaaagga gcttcctgac aatccttgcg acactttcga aaagcctaat acagatttga
    16261 ctgtaatgaa atctgtgaat tcccgataaa aaataatatt caagtacaac aaaagcgtat
    16321 ttaaaatatc aatgatcaaa agaatagcta acgctggctt tggcttttta atatacaaag
    16381 aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat ccacaagata aagtactgga
    16441 tcgtaccttc cacacctaat gaaaaatctg catagtaggc aatgatcgtc ttcagccaga
    16501 aaaagaaaac agctgtcaaa acgattgcta aacgattttg gcacaatttt ttaagttgtg
    16561 tcacgtatat cacttctctc tattcacatt ttttcttatt ttgctcctga aaaatgaaaa
    16621 tttcctaaaa aagttgttat cttaagattt ttttattctt tctaaagatt attccgtcca
    16681 cttttcacag cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc
    16741 ttgtggtgac cgcttcactc cacggtattc aaaagccagt ttctcggtat tcgtacaaaa
    16801 aaagaggctg tgacaaaatt cttgtcgcag ctccttattt cttgatgcag gacagctttt
    16861 tcacaacttc ttccttaaac ggtgttctcc tgccaactcc tcggccacaa gtcacaatat
    16921 ttataaaaca tgagaagctg ttttttgaaa ttgttccttg tggctcagag ttaaacggca
    16981 ggctcaagac ctcttgatac acggtgttca atcagctgac cctcggtatt agattatgtc
    17041 aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat
    17101 aagcccaacc aaacgaaaaa tataaggagc tattttttga aattgctcct tgtggctcag
    17161 agttaaacgg caggctcaag acctcttgat acacggtgtt caatcagctg accttcggta
    17221 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
    17281 ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc cataggattg atattgtcct
    17341 tgtgatgact cattcgtaat aattgaggct tgattttctt cacttcccgt tatcgtttgt
    17401 ccactgccag aatctgaagg acttgttcct gtcgactcgt ttttttgaga agagacagtt
    17461 gttttcttga cttgttcatt tgtctttttc cattccgcta aacgatcggc gaccactttt
    17521 ttgtctactt tttcagaata agcggtgatc acttttccat ttttcaaacg ggctactttg
    17581 tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt ctttaaattg atattgtctg
    17641 agagaaatcg cactaagtac gtcatcgata ttaataaatt gattttcttc ttttcctttt
    17701 ggcaaataaa tgtccaattc gcctttttcg taattggcag ttattttata ctccgcccac
    17761 caataagttg agaattcttt tttgaaatta tctactgatt ctttgcttgc tacgattttt
    17821 tctgtttctt tctgagcaag atctgctgtc atttttcctg aaaatgctgc ggataaaata
    17881 agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt cctcatgctt
    17941 tcctccactc ctatttaatt ttgactattc tattatatac aaaaaatgaa atagaaagac
    18001 aaaaaagttg aaaaagatcg taaataattg aacttctttt tcacaaaatg gtaagaatgg
    18061 agaatttcac gcatttgctc tcaactattc tttcttaagc ctcaaatccg tttttatctg
    18121 atacgtcttt gaaccaatga cccgattttt tgattgttct ttttccatct tgtgctaagt
    18181 ctactgaaat aaagccatat cgattcttat atgcattggt ccacgaccag ttatccatac
    18241 atgtccacat atgatagcct aaacaattgg caccttcttg aatggcttga tggacatatt
    18301 tcagatgaga ttggacaaat tcgatccgat aatcatcgtg gatcatgcca tcttctttga
    18361 taaatcgtgc ttcgttttct acacccatac cgttttcaga aataaagcag tcgatatttc
    18421 cataatgatc tcgtacattg atcaaaatat catagattcc ttgttcatag atctcccatc
    18481 cgcgataagg attcatttta cgaccaggca tttcataatt gtcgaaataa tcctcaggca
    18541 ttggaccgtt tttagtatcg attggtgttt cttttgcttt tacccggcgt ggctggtaat
    18601 aattgatacc taaaagatct actgtatttt ctttgataac cgtcaaatcc tctgcatgga
    18661 tcacaggcat gagatcaagt tcttttacta tctcaatcaa ttcacttgga aactcgcctt
    18721 ttacagctgg atcaaggaat gaacgattga agaacgcatc tgctatttgc gctgctttaa
    18781 catcttcagg gtttgattcg tctcttggat agcttggtgt cagattcaag atgataccaa
    18841 taacaccacc ttgctccatt tcatggtaag atttgatggc taacgctgaa gccaacgctt
    18901 catgatagcc aacttggatc gcttttttca tatctgactc ttctggataa tgccatcgat
    18961 ataaataacc tgcttcgact ggaacgattg gttcattatg agtaaaccat ttttttactt
    19021 tcgaaccaaa caattcaaaa caacttttgg catatttttc ataggcttca accgtttctc
    19081 ggttcaacca tccgcctttt tcttgcagat acatcggcat atcgaaatga tacagattca
    19141 caaagggttc gataccgttt tcaatcaact catcaaaata acgatgatag aaacgaacgg
    19201 cttcttcgtt gattgttcca gattcatcag gtatcaaacg gctccattgg atagatgttc
    19261 gaaatgaatt atgcccagtc tgtttcatca actgtacatc ctcttgatac ttcgtataaa
    19321 cttgcgaggt tttttcaggt cctacttgat caaagaactt ttgcggttct tttttgtacc
    19381 aataatcaaa aatgttctct ccttttccat cgcctgaaat ccttccttct gtttgtgggc
    19441 cacttgctgc agaaccccac caaaaatttg ctggaaattg atattttttc atcaaagcac
    19501 ctctttttct tttttagttc gttttagttg attccatgta tgatattttc catcacaatc
    19561 actttttcat tttcttcctc ttcttttttg attcgtttta acagctgttc tgctgctcgt
    19621 tccccgattt tatctgttgg tggcgtgatc gtgattttcg gaaggatact cagatcgttt
    19681 ttatctaaat aatcatagtg actgaacgca atatccttcc cgattctgac accttcttga
    19741 ttgaaacatt cgatggcacc aatcgacata ttgtaattgc tgacaaaaat agcagttgcc
    19801 cctttttcta ataatgcttt tgccccgtaa tagccgcttt ttctagaata gtctcctgta
    19861 aagacctcta attggatcga tggatcattt tggaccgctt ctttggctcc tgctaaacgt
    19921 tctcttgcta cgtagtctgt ttgagtggca gcaataaatc cgattttttt atgtccttgt
    19981 tcaccatgat gagcgatcac ttttgctaca ctttctcgat cattcactaa tattgtatca
    20041 atacctgctg gtccttctgg acaattcaat gcgataactg gaatactcat ttttgtgagc
    20101 atttcaaatc cagaccactc ttcttcggat aagaagacaa tcagcccatc gatcacatgc
    20161 ttcatcaggt attcaatttt ttgttcaatt ttttctggct gatcggcaaa cccgcttaat
    20221 aataagctat acccattttc ttcagctact ctttcgatcc ctgagacaac ttccgaccca
    20281 aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga agcttcgatt attcttcaac
    20341 cctttagcaa tgatgttttc tttatagtcc aacgctgaaa tcgcttctgc tatttttttc
    20401 atgttctttt ccttcagttg ctgaccattc aagtaacggg aaactgttcc cactgaaaca
    20461 ccagccatct tagccacatc acggatcgat gccatacatt ctcactcctg ccagttacat
    20521 ttgatttctc tttcttgttg ttcgattatg atgaccgcat tctttgcata gtgttgcttg
    20581 tcatcaatat ccatataact aaagtcttca gtagagcaac
//
LOCUS       DAHEYX010000030        20268 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHEYX010000030 DAHEYX010000000
VERSION     DAHEYX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.030"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            664..1008
                     /locus_tag="MB219_000752"
     CDS             664..1008
                     /locus_tag="MB219_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCC8426487.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1013..1294
                     /locus_tag="MB219_000753"
     CDS             1013..1294
                     /locus_tag="MB219_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426488.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            1397..1711
                     /locus_tag="MB219_000754"
     CDS             1397..1711
                     /locus_tag="MB219_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCC8426489.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1689..3383
                     /locus_tag="MB219_000755"
     CDS             1689..3383
                     /locus_tag="MB219_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCC8426490.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPGLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENAGTGFTPLVF"
     gene            3403..4581
                     /locus_tag="MB219_000756"
     CDS             3403..4581
                     /locus_tag="MB219_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCC8426491.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRAQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYKLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4544..5230
                     /locus_tag="MB219_000757"
     CDS             4544..5230
                     /locus_tag="MB219_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCC8426492.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKREEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5230..6390
                     /locus_tag="MB219_000758"
     CDS             5230..6390
                     /locus_tag="MB219_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCC8426493.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTRA"
     gene            6400..7275
                     /locus_tag="MB219_000759"
     CDS             6400..7275
                     /locus_tag="MB219_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426494.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7272..7583
                     /locus_tag="MB219_000760"
     CDS             7272..7583
                     /locus_tag="MB219_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426495.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7573..7926
                     /locus_tag="MB219_000761"
     CDS             7573..7926
                     /locus_tag="MB219_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCC8426496.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7916..8317
                     /locus_tag="MB219_000762"
     CDS             7916..8317
                     /locus_tag="MB219_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426497.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8310..8714
                     /locus_tag="MB219_000763"
     CDS             8310..8714
                     /locus_tag="MB219_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426498.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8726..9334
                     /locus_tag="MB219_000764"
     CDS             8726..9334
                     /locus_tag="MB219_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCC8426499.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9354..9716
                     /locus_tag="MB219_000765"
     CDS             9354..9716
                     /locus_tag="MB219_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426500.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9719..9901
                     /locus_tag="MB219_000766"
     CDS             9719..9901
                     /locus_tag="MB219_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426501.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9918..13337
                     /locus_tag="MB219_000767"
     CDS             9918..13337
                     /locus_tag="MB219_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCC8426502.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAVATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13388..14125
                     /locus_tag="MB219_000768"
     CDS             13388..14125
                     /locus_tag="MB219_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCC8426503.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFTCTGLSKIQIYRNGLR"
     gene            14135..16426
                     /locus_tag="MB219_000769"
     CDS             14135..16426
                     /locus_tag="MB219_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCC8426504.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16450..18693
                     /locus_tag="MB219_000770"
     CDS             16450..18693
                     /locus_tag="MB219_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426505.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPFQPNLLAEPYNMCREYPNENIVNPTVKF
                     PIKTSNNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQHQATLLP
                     VEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEKGNTRTPNIS
                     EYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQ
                     LTASINEVDTTAKDARTKANANATAIDELDNKIDERINDAATTAFYGAQGEVSNKTKV
                     AEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLY
                     KDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTL
                     VLTRLSD"
     gene            18771..19013
                     /locus_tag="MB219_000771"
     CDS             18771..19013
                     /locus_tag="MB219_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCC8426506.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            19029..19226
                     /locus_tag="MB219_000772"
     CDS             19029..19226
                     /locus_tag="MB219_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCC8426507.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19237..20256
                     /locus_tag="MB219_000773"
     CDS             19237..20256
                     /locus_tag="MB219_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCC8426508.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         7155 a         3018 c         4286 g         5809 t
ORIGIN      
        1 attattcaca cgataactaa aggtgggtga agagaaatga ttccattaat aatttcaatt
       61 tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag
      121 tagcagccaa gaataatttt atagtgtcac tgtggcggaa agggtagacg ctaagcatgt
      181 gtgctaggtc aatgcttcgg caaccatgca aggttcgatt ccttgccagt gactttggca
      241 accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat
      301 cgtgtaggtt gctattacat aactggttag gttagattga gatttgggat tcggtacaaa
      361 tgaatcgtca aatgtctcaa gcacaggatc ggaaacgtcc ctgcctgtgc attacatatt
      421 agatcactcg ttgagtggtc tttttatttt gcaaaaagga ggtaacaaca atgtatagac
      481 cacaatactt agaacagaag catgaagtaa tcactgtgca aaacggtaac ggtgagatag
      541 tacgaaagta tagaagacca ataaagagca atacatataa acgaaaggaa agcaatgaag
      601 ttattccatt gtatggcaaa agaatagcta agtattaaat aagattgcga aaggagatgg
      661 gacatgaccg aggaattcta tagatggtta ttacagttga taagagaaga tcgtttggtt
      721 aagttctatc agtctcctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac
      781 tatgaatgcc aagagtgtag aagactaggt aagtatcata gagtagagaa cgttcatcat
      841 ataaaggaag tcaaggatag acctgactta gctttagatt tagataatct tatttgttta
      901 tgtgttgaac atcataatga agttcatggc agatatctta cagcattaga taaacaagag
      961 aagaagatag aaagctttgc taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat
     1021 caatgacaat ggcagagagt atgacacaga aaagattgaa gagtattcat cttatactca
     1081 aggattaatt aaacgtttga tatacgttcg ctatgtaggt attagggatc tgttatcaga
     1141 taactgttgc agtaaataca aagtgaatca agtaagagaa gcgttgaata aagataataa
     1201 cgttgaaaga ataaaaaata tttttggata tagcattgaa gagattaatt attacattga
     1261 cttcgctgaa gctttcattc cgatggtgag ataacccccc cttaaaataa atcgcaaatt
     1321 ttttgggggt gatgaaacgg agggggctgt caggaaaaga gattttttcg aactttatca
     1381 tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt
     1441 acgccgctct ttaataagcc aattggaaag tcagaaaaca aatattgagc cattcttaga
     1501 taatgttgat cgttatatca gtttatggga aacggcgata tcactggaag aagatatatc
     1561 cgagaacggc attagactgg agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc
     1621 tgtcaacaaa caaatgggat tgatgttgga taaacttgcc attactcctg aattggtagg
     1681 tgaagcaaat gaatcaattc ctgagttata agcatattga aaattggttc aaagctatag
     1741 aagaaggcac tatcaaggta tgcaaagagc aattattgct aaaaaattat ctagaagaaa
     1801 gagtctttac tagagaagat atttacttcg ataagcagat ggtagaggat tcaatcaata
     1861 taccagcaca atactttcca ttcgaattaa ttccgtggga aaaatttcta caatgtttta
     1921 tttatggtgt tcgatggaaa aaagataaaa cgctagtgtt caatagatat ctttcattaa
     1981 tgggacgtgg taatggtaaa actggttttg cttcttggaa caattttttc ttgctgactg
     2041 caaaacacgg tattaaaaat tatgatattg atatctatgc caataacgaa agccaagcaa
     2101 agactagttt tgatgatgta tttaaagtaa ttaaagatca tcctggttta gataaaaaag
     2161 tatttaaagc tacgaaggaa gttattcaaa atatcgctac aaacagcaaa cttcgttata
     2221 acacggcaaa cgctagaaca aaagatggta aacgaccagg agcaaaccgc tttgatgaaa
     2281 ttcacgaaaa tgaagattat tcaatgataa atgtagctac ttctggtggt ggtaaaattc
     2341 gagattatag agaattttat gatacaacta atggtcatgt tcgtggtggt ccgcttgatg
     2401 acattataga agaatcaaaa atgattcttt ctggagaact tggaattgac aaggatggag
     2461 cagaattttc tagtttgttt ccatttattt gtcgcttgga taatgataat gaagttgatg
     2521 atcccgatat gtgggaaaaa gcttgtccaa ctattaatta caatgcagat ttaaaacgga
     2581 aaatgtttca agaatactct caaatgcaac gtaatgctgg tttaagactt acgttcatga
     2641 cgaaacgaat gaatagacca atggaagata cgcgatttgc tgtagcttca tatgatgatg
     2701 ttcttcatac gaaagaaaaa gaatttcctg aaaaaatgga tgaagtgata ggaacagttg
     2761 attttgctga tagacgagat tttgccagcg ttggtttgtt aggaaaatat gataaagatg
     2821 tgtattttac acaacatact tttatccacg aatcagctct tcgattacag aacataaaac
     2881 gagaggttat agatatttct atagatcaag gaaaatcaca gatcgttcat gggaaaaata
     2941 tagaagctga ttatattgta ggttggtttc ttgaaatgag taataaatat tacattaaaa
     3001 aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt
     3061 ttactgtaga aattgttcga agcggatctg ttacacatgg tatgttaaaa gacctagttg
     3121 atgacctttt tattaatcaa cgtttatttt ttggtgacga tgcgattatg cgttggtatt
     3181 gcatgaatgt atatgaagag catatttcta atggaaatat acgctatgaa aaaatagaac
     3241 ctgaaactag aaaaacggat ggcttttttt cattccttca tggtttgaat tttttagatg
     3301 atatttatga ttctgctcct gtaacagtca caaatagttc agtagaaaat gcaggaactg
     3361 gatttactcc tctagtattc taacttgaaa ggaggtgaga aagtggggat ttttcaaaag
     3421 gcggtaggat acttcacaaa aaaagcaacg gttcctttag aagaatactt ttgtaaattg
     3481 caagttgatt ttgtgtatcg aaaatttgca attgaaactt gtattgattt gattgcaaat
     3541 gcgatgagca aagcggaatt caagtcatat gaagatggga aaaataaaaa gaatgatctt
     3601 tactataggc tgaatgtagc tcctaataag aaaaataatg caacagaatt tagaaaaaaa
     3661 ctgatcagga gattaatatt ctacaatgaa gtattgatcg tttctccgtc taataattct
     3721 agcgaaatat ttattgcgga tagttgggat gtcacagagt atgcattaaa agatgatgtg
     3781 ttttctcaag tgcaaattaa caacatagtc cttgatagag aatttctaga aagtgatgtt
     3841 atctatataa aatacgcaga tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg
     3901 tatgggaaac tcatttctag tgccatgaat gtttacaagc gttctaacgc tcgtagatac
     3961 gtactgaaag ggaatttatt ccgagcgcaa gacaatacaa cacaagatca aatcaataaa
     4021 atgatgacat cacaatttaa ggcttttatg gaagctgata atgcaggtgc ggtatttcaa
     4081 ttacaaaatg agtacaaatt agaagatttc agcggaaact ttcaaagcaa ttcaagagat
     4141 ataaaaaact taatagacga catctttgag atgacagcag cagcgtttca cgttccgaaa
     4201 aacctactaa agggagacat gagtggatta tcggatcaag tggacgcttt tttaatgttt
     4261 gaaatcatac cgattgctga acttattcag gatgcgttta acgctagtct ctatgaagta
     4321 gaagaatact tgtcagggaa ttttgtacgt gtggatacaa ctatgatcaa gattactagc
     4381 ttcaaagatt tggttgacgc tattgatgta ggcattagaa atggggtatt tacaatcaac
     4441 gaaggaagag aacgcgttgg aaatgatcgc tctgataagg cgatggcaga tgaaatattt
     4501 ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac
     4561 atttctagca gtaaagaatg aaggcgcagt accgcaaatt tttattcagg gatttattgg
     4621 ttctagttgg ttctttgaag ggaatactga caagggaatc aaaaatattt tggatagtct
     4681 aggtgatcaa gaagagattg aagtagtaat taattcaaac ggtggagacg tatttcaagg
     4741 gattgctatt gggaacttac ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg
     4801 cttagccgct agtgctgctt caattatcgc aatggctggc gatactataa aaatctacaa
     4861 caatgcacaa ttgatgattc accgcgcttc cacatacgga gaaggaaatg tcgatgactt
     4921 ccgtacgatt gctgaccaac tggaatcaat tgataaatcg gtaaaggctt catataaaac
     4981 acgattcaat ggcacagatg aagcattgca agaacttctt gaaaaagaat cgtttatgga
     5041 tgcagaaaca gctttgagtt atggattggt cgatgaaatt atcgatgcag aaaatagctc
     5101 aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa
     5161 aagagaagaa aaaattgctg catttacagc agcattaaat aaaacatttg gacaaggaga
     5221 tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg
     5281 aaagctttta aagatggtaa cgaagaatct tttagcgcag ctatggtaag cttatctaag
     5341 gaaattcagg ataaaatttt agaagaagca acagcaaaaa atcaagatca attagtatta
     5401 atgaaccgtg gtcagcgtgt attaactaca caagaaacaa aattctataa cgaagtagtg
     5461 aaaaacgaag gttttgcagg ggttgaagaa ttagtgccag ctactgtatt tgaacgtgta
     5521 tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa ttacttttgt taacacaact
     5581 ggtgtaacag aatggattgt gtcacgtgga gtcaatccag catggtgggg taaactttgc
     5641 gaagctgtta aaaaagtttt agataatggc tttgatgtaa ttaacatgaa gcagttcaag
     5701 ctatcaggtt atattcctgt atgtaaggca atgcttgatt taggtccagt atggttagat
     5761 cgttatgtcc gtactgtttt agtagaatca ttgagaattg cattagaaca agcaattgtt
     5821 gatggtactg gtaaagatat gccagtcgga atgatgcgtg acatgagcaa acaaactagc
     5881 ggagaatatg ctgaaaaaaa agcagaacct attacagctt tagatgctgt aactatgggc
     5941 ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt
     6001 aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa ctgattactg gtctaaagta
     6061 ttcccagcta agactgtact aactgctaat ggagaatacg tacaagtatt gccagtacca
     6121 gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag cagttattgg ggtagcctca
     6181 gattacttca tgggtgtagg atctacacta aaaattgaag cttcagatga ataccatttt
     6241 gttgaagacg aacgcattta tctagctaaa caatatgcaa acggtcaacc taaacgtaac
     6301 gatagtttca ttgtattaga tattagcgct ttgggaacta ctactacaac tacaaaacca
     6361 acaaccacaa caactacaac acgagcgtag gtgatcagaa tgaagtatat tctttgtcag
     6421 ccggcaatca atcggtttaa atgggagctt gaagtttgtt taactaatct gaagaaacta
     6481 ggaatcaaag atatcgtatt gcttttcagc agacacgatg atcagattcc tatttttttt
     6541 gagaaggaat atggtgttga agttcatgtg tacgatgatc tgcgggacga caaagagtat
     6601 attccttcga ttaaaccata tttatggtgg aaatatttag aagaagatca ttcgcgtgag
     6661 gacgaccgat atttctatat cgattcggat gtcattttca ataaaagaat taatttgcgc
     6721 aaattgcctt ctaaagatga tgtttggtat tgtagcgact gctgtagtta tctaagtctt
     6781 gattatatta gaagctgtga aaacggagaa aatattctaa aagatatggc aaacattgta
     6841 aatgttacag tagaatcttt ggaaactata aacactaatt caggaggcgc acagtgggtt
     6901 attaaccgtc ctaaagcgaa ttattggaaa aaggtttatc tggattctaa tcggctatat
     6961 cgctacctta gagggcaaaa aacaaatata caaatctgga cagccgagat gtgggcacag
     7021 ctttggaaca tgatgtattt caatattggt cctaaagttc acgaggaatt agacttttgt
     7081 tttgctactg atccaataga aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta
     7141 acaacaaacg atgaagattt atttttcaaa gggagatacg tgacttctac gccttttgat
     7201 gaagatttat catttgtaaa caagaaaaaa tgctcttacg catatgttaa agcaattaag
     7261 gcggtggtta gatgacgcct gaacaagtga ctgaagaatt gctaatagct gtgaaggata
     7321 atatttacgt tacctggaac gaagaagatg agtcaattaa aaagatgata gctaaaaatg
     7381 ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt ttctcctgaa agcttagaat
     7441 acggattgct aatcgaaaga tgtagatacg actggaatcg tgctttagat gagtttgaac
     7501 aaaatttcgc tagtgagtta ttaggtttca ttcaacatta tgcgctacaa gaatatattg
     7561 caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa cattcaacga tggttggtta
     7621 aagattttga cgcaaactac caaaagaaat gaactaggaa aaaagattgg tgtagaagat
     7681 acagaaatca cttctttaaa atttagaaat ctttccatga gagatagtga tataacagct
     7741 atggatgcga tgggatcgaa attaactaag aaagtaaaga ctccatttca tccaatcgcc
     7801 aagaaattta ataaagatca atattttatc gtaatcgata gtatgcgtta caacgttatc
     7861 tatgccgatt acgataattt ttatatctat ttttatcttg aaagtgtggg tgaatatggt
     7921 gattgataat tctaaagaaa aagaacgttt aaataagcaa atttctgcta tcaaaacttc
     7981 cttagaagag cattttggcc tcaaactctt tcaagactcc gtaggcgagg atgagctacc
     8041 tgatgatttt aattacttca ttctcgaaac aggagaaata gaaatgatca ctgagccaaa
     8101 atatagcgtg ggtcaaaatc tatatctaac tttctattca gaaaatagag aagatttaac
     8161 aggagactca ctagatatta tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat
     8221 ggatcccaat catttaaaac tagagaacca agatcgctat atcgatcaat tggtatttac
     8281 gtttagacga ttattgaaga gtgattgtca tggctaaaaa tagttgggag ctaaaaataa
     8341 atggacatga tgaacttctt gtgcggatgg aacgctattc aagcgagagc gaacgactga
     8401 ttaacgaagc attgaaatca aaaggttcgg ctattgcagt ggataggatt acagaaaaaa
     8461 ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac
     8521 ttaagactca atacattaat ttgggtttca tcattagacc tacaagaaaa tttgagtatt
     8581 taaaatatcc tgatttgggg ataggtactt ctaaaagaaa tcagccggac gaatttatga
     8641 gaagaggatt aggtcttgca cttgatccaa ttacagaact tctgattcgt caattcgata
     8701 aattaaataa atagggggaa caacaatggc taaaacaaaa actgtagtaa caacgttcga
     8761 taacgtaagt atcaaacgaa ttgcttttaa ttttaagaac gcaggaaatg caatcgcaac
     8821 gaattgtaac ggacaattag atggcgaaac agaaatgcaa acggtggtta aaaaatgtgg
     8881 agcgacagaa gtaaaatcaa aatctaaacc aatcaatatg acggtaacaa ttactgcaca
     8941 tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa caagatggac gtattaaacc
     9001 aggcatttac tcttacggtc ctgattccgt aggcgaagat ttctcacttg ctgcagagat
     9061 cgtggatgac ttcgaagaaa atagaaagtt agttggtatg ttagcatgca cttcgaatac
     9121 aggattaaca ttctctattg aaaatggtgc ggatgaagta gctgcgttag aactagaaac
     9181 aaaagttatg caagatgaat ttggtaaatt ttatcatgaa gcaattgttg cagaacttga
     9241 agaagactta acagatcaat ggatgacgaa tctatctgct gatgtgatta aaaaaacttc
     9301 agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg
     9361 aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa cctaaactga
     9421 atatcaagaa attattgatg atcaatagag attttaacac agacgagttt gcaaaaatga
     9481 ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct gtgtatattg
     9541 cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc gataaatggg
     9601 attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa caagcacgcg
     9661 atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
     9721 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
     9781 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
     9841 caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatctc agaaaaacta
     9901 aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaact ttcatagcta
     9961 acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact aaaaatagag
    10021 cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca gacaagttag
    10081 gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagccaaaaa gttgaagtaa
    10141 ctagccaacg tttagccaat gccaaaaaat attatggaga aaattccacc gaagttcaga
    10201 aacttgagag agaactgatt aaccaacaaa cagcgcaaca acgtttgtca aacgaaattg
    10261 ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac gagtctacaa
    10321 tgcaacagtt ggatagtgaa caaaaaaatg ttcaagctag tgcttctctg attgaatccg
    10381 aatacaaaaa atggcaagta actgctggtc aatcagcttc tgaatccgag aaattagcga
    10441 aagcccaaga atatgtttct caacaatctg aaaatgcaga gaaaacgata gatatcctga
    10501 gacgacagtt agaagctaca cagtctgagt ttggcgctac atccacagaa gcaatgcaga
    10561 tggaggcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa
    10621 atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat aatttaatgg
    10681 aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg aaacaagcag
    10741 tggactcagc taacagtgta ggtagttccc agagtaagat acaagctaat tttggtttga
    10801 ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat aaaggttttg
    10861 gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat ttaggcgatt
    10921 taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa aacaccatgg
    10981 gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat ttcggcttga
    11041 gtgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta gataaaacgc
    11101 acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg ggatattcag
    11161 ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat aaccttgata
    11221 aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga tttgagcaaa
    11281 acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa aatggcggag
    11341 gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg gatggtcaat
    11401 acgaccaatt aaataaagct tctacaattt ggtctgcact tggcgaagat aacgcgatga
    11461 aagttgtcca atctttgact gatgttaacc atacatttga tgatgttagt ggatctgcac
    11521 aaaaaatgaa tgaagattct actactccgt tacaagaatt aaacggaaaa atagctgaat
    11581 taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa ccagtaattg
    11641 attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta caggattatg
    11701 ccgtagcgat tggcggattg actgctgcat ttactttatt aatgccaata atagttggct
    11761 tcatggctct aggtggtcct actacattaa taataggagc agttattact gctattgctg
    11821 gagttatagc aattataaaa aactggggcg caattactga ctggtttaag ggaatatgga
    11881 gtaaattcac tgattggttg ggtggtactt gggaaagcat aaaagaaggt gcctcatcag
    11941 tttgggatgg agtcaaagaa acctggtctg gatttgtaga ttgggttcaa gaaatttggc
    12001 aaggagtttc tgattggttt ggagagttat ggagcggatt agttgaagga gcttccaaca
    12061 tctggcaagg agtccaagag acttggcaaa cattcattga ttgggtttca aatatttgga
    12121 acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt gttcaaatac
    12181 catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg ggtatttttg
    12241 atgtagctgg acagttatta ggcgcagctt ggcaagctgt ttggacacct atttctgatt
    12301 tccttaaaaa tacttgggat actatgacac aatggataag catcgcttgg aatggaattg
    12361 taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt atatggacat
    12421 ctattagtac tacggcttca aatatttgga actcaattag tgcaacagct tctagtattt
    12481 ggaatagtat caagaataca atcactagct tggtacaagc agttgctaca gtaattcaaa
    12541 atatttggtc aactgtatct agttggttag gtggaatttg gaattcaatc agctctacag
    12601 catcaaatat ctggagtagc gtgactagta gtataagcaa tgctataaac gcagctaaaa
    12661 gtgccattca aagtgtttgg aatagtatat cttcgtggat cagcggaatt tggaacggta
    12721 tcaaaaacac tgctttgaat ctttggaatg gaattacaag cactattagc tctaaagtaa
    12781 acgatggaaa aaatgcaatt tcaagcggtt ggtctaatct aacaggtatt gtttccgaca
    12841 tattcaataa tgttaaaagc acgatagcta atatatggga aggtatcaaa aagactgtta
    12901 gcgctccgat tgattggatt agagataaaa tcagtagtgt ctttgataat ttgaatattt
    12961 ctataccaca tattccgtta ccacatttta aattgagcgg agaattcaat ccattgaagg
    13021 ggaaaatccc aacattgggt gttgattggt atgcgaaagg tagtgtgttt aattctccaa
    13081 atattatcgg tgtcggtgaa gcaggacctg aagcagtttt acctttgaaa agatctgtgc
    13141 tgcaagaaat tggggatcgt atcttgagta gcacctcagt ttcatctagg gcacaaacga
    13201 ttcaacctgt gaacaattac gaattcaatt tcacaattga tggtaacgca gatgaagtca
    13261 ctatgaagca aacaactcaa caaatcattg atagcattac aaaagttcaa aatgataatg
    13321 cttcggcatg gcgttaaaca ggagagtatt tctcctgttt ttttagtttt aaaaaggatg
    13381 tgaaaaaatg actgattgta tacattctat aatcgatgga tttcctgatt atttgcataa
    13441 attggcttta gcggaaagac caaccatacc ttctccaaaa agacagagag ttgaaacttc
    13501 tgttttagga cggctaggtg gcttagtaca agattactcg tttgaagaca tgtcgtttac
    13561 attgcactat aactatttag aagatgtgga agaccatcaa gcattcaagc aatcgtttta
    13621 tatcatgcgt cattggttaa actatgcaaa gaaattagaa ttctctgatg atcccaacgt
    13681 ctattatgtt atccagacta tcgatattgg ggatgcagaa aacgatattg ttgaatgggg
    13741 agagttcgat gtaaatatca ctgcgaaacc attcgcaaga gttcaagaag atgtacctat
    13801 aaccgtagat aaaccacagt catttaactt gctgaataat agtttagaag aaagttttcc
    13861 aaagattatc atcactcctt cagctacttc atgccagttc atcttaaatg attatgtgtt
    13921 tagttttgaa ggcttagtag taggaactga tgtagtcatt gatagtgatt taatgctttg
    13981 ctacgaagag caatcggacg gagatatttt agatcggtcc aacaaaatga agaccatgca
    14041 atatccaacg ttgcaagtgg atattaatca ttttacttgt actggtttga gcaaaataca
    14101 aatttatcgt aatgggttaa ggtaggtgaa atagatgatc gataatttac taactattta
    14161 cgataaaaat gacgcgaata atttagctga acatttatat gatacgcaag gtttaggcgc
    14221 tttgtcagac tggttaacag ctactgttag caataaacta aacggagccg agatatttca
    14281 gggtacttat tcaataaacg gaactaatgc agatttgatt gtagaaggac gtattattca
    14341 gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg atttattatg caaaaacttc
    14401 tgtaataggc aatacgatag aagtaaaagc tgaacctatt ttcaatgata taagaaaatc
    14461 ggtgttgaat aaatatgaca gcggaacaga aaaaatcact gctactcagg catggcaaaa
    14521 cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt tctttctcgt cattagtaga
    14581 tacgcttgct aatgtgaaga tagaaaaggc gaatttttta gaattctttg gtggaaaaga
    14641 gggatctatt ctagatcgat ttcatggtga gtttctaaaa gataataaca cattacgtca
    14701 tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt tatactaaaa acttaactgg
    14761 tcttgactta gagattgatg ctcaaagtgt tttagttgga gtttatccat ttattagcag
    14821 ctcttcagaa ggagaagacg agatcactct accagaagaa gttattttca cggattacgt
    14881 ggatgattat cctgctggat atgtttcttt tgttgatttt aaagacaaag cgactgatgt
    14941 agccacatta agggaagctg ctaaagactg gttgaaaaca aacatagata aacaaaaacc
    15001 acaagtgagt ggttcgattg aattagtacc attgaggcat caaagaggct atgaaaaatt
    15061 tgttgatcta gaaaaagttt cgatgggtga cggagtagat gtgtatcatc cacagttaaa
    15121 agtgaatatg tcagcgagaa ttgtggaata tacgtttaat gttctaacca attcatacga
    15181 taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa aacacagaga ataatgtcag
    15241 caatttgatt aatgatgcca ttgatcaatt gaaaaacgtt ggcgaaatca gtgatttaat
    15301 caatgatatt gtagatcatc aaactgatat gattactggt caaaatggtg gttatgtttt
    15361 attagatcct aaagaagcgc ctagtcgtat tttgattatg gacacaccag ataagaatac
    15421 cgcaaggaat gttttacaaa tcaacaatgc tggtattggt ttttctaaaa ctggcattaa
    15481 tggaacatat gaaacggcat ggacgttaga tggcggattc aatgcctcgt ttattacggc
    15541 tggtgaaata gtaggaatta ctattagagg tactacatta attagtgatg gtgctgatta
    15601 tagaacaagt attgctaatg gcaaaatgac ttggtactca aaaaaagtta acaaagatat
    15661 tatggagcta gaagcacgtg attatgtaag tgctgatgcc ggtattgtat catacaccat
    15721 gaaaactggt ggtggtttca tgattagaaa tccacagggt aacttggttt ttagtacgtg
    15781 ggataatggt aataacagac cgtttctatc ttttggtgcg cccaatttca ggtatagcaa
    15841 tgctagttat gtaacttctg gcgacggtag ttctttaagc attaatggta gtgcgggtaa
    15901 ctcatgggaa tttaaggtag ctggtaggac tatgaaattt actagtgatg gtatgctaac
    15961 gttaccaggt tgtttttttg gttcatggga agatgggaaa cttgctaggt ttgaacaatc
    16021 aacggtacaa gtatataaag attttactgt tagaggtact aaaaactcaa ctgtaccaac
    16081 agaacattat ggacaacgac tattgaacgc ttatgaaact ccagaatatt atttcgctga
    16141 ttatggggaa gccgttacag gtgacgatgg taaagttcgt gttgatattg accccatgtt
    16201 tgctgagaca gtaaatctaa gtcggtatat gacacatgtg acacctacag aactagtttt
    16261 gtgtgctgtt actcatgaag atattgacca tttcatcatt gaaactagta agccaaacgt
    16321 attagttaga tggaatttag tggcacaccg tctagggtat gaagatatta gattaaaaga
    16381 ggatacagca tatgatagca cagtgcttga ccaaaaacgt ttttaaaacg aagacaagga
    16441 ggtatataaa tggctagcag tttatataat ttggctttag atttcagcaa agaattaaac
    16501 tacaccaaag ctattatggc tcgtcagggt gataaaggga ttacggtgac ggttaaacca
    16561 tatttaaatg gcttgcagat ggatacgagt ggcggaacat ttactttaaa aggaacaaca
    16621 ccatctaacc gttacgtaga taatgttgca actagcgtaa ctagtgaaga agtcacgttt
    16681 tctcttgatg gcacatttat gagtgaagca ggatattata aacactgcta cgtagaatat
    16741 agaaaagaca atcaaattct aacaacgcaa gatatcattt ttttctcact aggagtgtct
    16801 gacatttcgc aaggtcaagc cgatgaatat gtttcgcaat tagaagagtt gattcgaaag
    16861 tataatgaaa cttttgatgc ttttatggct gaaactaaag gtagagtgga tagcttaaat
    16921 caacagatta ctgatttaac tggtcaagct aaaacgctac aagacaagtt agatgctctg
    16981 aaagaagaaa tttctaagtt aggtaactta caagtgatgt acagtaacag catcgatttc
    17041 gggaactatg attatagtgg gaatcctaat ttgttaagta agctatcata cgacttaatt
    17101 gaaaatcaaa atacttcagc tggaacactt tctaaaggtg aaaactcgtt taaatataat
    17161 aagatatcag ctgaaacgga aggtggagta gagttatatt ataaacgaag aggtatagct
    17221 aactggttac cctctaataa aacgcttgta atgaccgtta aacttagagc tggagttgac
    17281 tatagtccag ctgacggaaa aaaaatactg attagatata ggtttgttga caatggaact
    17341 ggcaagattg ttttagactt acctattaac agtaattcga taactcagga atggaaagag
    17401 tttagtatta ctggaactac tccaacattt ggcccgcaag cataccatcc ttggatacaa
    17461 tttagggctc aagatgggat acttggggaa atagaaatga gctatgacat caaaatcgaa
    17521 gaaggtccaa cagccacacc gttccaacct aacttattgg cagaacctta taacatgtgc
    17581 cgcgagtatc ctaacgagaa cattgtcaat cctacagtta agttcccaat taaaacatca
    17641 aataatccta tttctggttt tgatatttca gaagaattaa taataggtga aacttatacg
    17701 gtatctttaa aagggactaa gccagctact aaagaattcc acttatacta cggagaagca
    17761 aattaccagc agttgcagca tcaggcaact ttacttcctg tggaaggatt aacagatgtg
    17821 tggagtgcca cttttacagc cagaaatact agtgaaacga caaatttact tagagtagcg
    17881 ttatggcaaa aacctaattc tgcaacatat gatactgttc aaattgactg gctcaagatt
    17941 gaaaaaggca acacccgaac gcccaatatt agtgagtata aatatcgtgg tactggtatg
    18001 cgtgattcaa acaatccaaa agattatgtt tgggatctag caccagaata tgtcgaagat
    18061 aatttggcca cagatattaa aatttctgaa attactggta aagcaaacaa ttataccgat
    18121 gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt agacaccaca
    18181 gctaaggatg ctcgaacaaa agcgaatgct aatgcgactg ctatagatga attagacaat
    18241 aagatcgatg aacgcattaa tgatgcagct actaccgcat tttatggcgc acaaggtgaa
    18301 gtctctaaca aaacgaaagt agcggagtat ggagtaggaa cagggtatac tactacagct
    18361 gtaggagaaa cacatttcga acgtcaatct aacggatacg ttaaatgctt aaaagctggt
    18421 aagtattcaa ttagtgcgca aatacgtgtg caattgggcg ggaaatatgc ttcatggtta
    18481 tatactgatt tatataaaga tggaacttct gttgataatt tagtatctta tggggtcgac
    18541 gccttgcaaa atagatttgc agctagcgga aacattgtaa ctgatttaga agtaaacgat
    18601 gtactaaata tgcgtactga aatcggcgtt gcagatggaa ttttacaatt tacatcttgc
    18661 cgtactttag tcttgactag actatctgat taatacaata tttataattt tgctatcaac
    18721 acgctcaaag gagggtgttt tttattttgc aatgaaagga ggctagttgg ttgaaagacg
    18781 aagcaataca agacgtggta gaacgcttag tgcgtattga aacgaaactg gataattacg
    18841 aattattacg tgaaaaagcg gaaagtgcaa aagatagagc ggatcaggta tattctattg
    18901 cgcttaataa tgcagaagac atcaaagaaa tgaaagccaa taataaatgg tcgtggggtt
    18961 acatgattgg tttaggcatt acgatcattg gctatttctt gactaaattg taaaggaggt
    19021 gagaagaaat gattttacca gataagtatt atcaagtcat taaatggaca gttttaacag
    19081 tattgccagc tgcatctgct ttagtagcca tgttagggaa agcgtatgga tggaatggaa
    19141 cagatatgat agtgctgact atcaatgcag tagcaacatt tttaggcgtt atcactggtg
    19201 tgtcggctta taatttgaaa aaataggagg aaacaaatga aaaagaaaat tactattact
    19261 gcgatgagcc tgttaacggc tcttttttta ttgccaatta acggatttgc ctatactatc
    19321 aacaatgaat ttaatttggg cgcaaatgaa ggtagctcac aagtagcaaa taatcagtac
    19381 attttactgc atgaaacggc taatgaaaca gcaacaggac gcaatgaagc gcagtatatg
    19441 caacgttcat ggactagtgc ttacactgct tacattgtgg gtgatggcgg aattgtttac
    19501 caagttggac aacctggtta tgtacagtac ggtgctggtt cgtatgctaa tgccaacagt
    19561 cctgtgcaga ttgagttaca acacacacat gataaagcaa cgtttgagaa aaactacaag
    19621 gcatacgttg aattggctag agattcagca atgaaatatg gtattccatt aacgttggac
    19681 actccttata accaaccggg aatcaaatcg catttatggg taacacaaaa catctggggc
    19741 gatcatacag atccttatgg ttatctttct gaaatgggcg taagtaaaga aaaattagca
    19801 tatgatttag ctcatggatt taccgatgaa aatccgacaa cttcagatga taaaccagtc
    19861 attgatccaa ctagagcagg tgcagcaaat cctacgctga cagatggaac aaattacgcc
    19921 cacattgatc agtttgggga aatcgaaaac gcaaacttgc atgtggctgg atggcacatt
    19981 gctaactata aatacgagta tatcttcatt atggactaca atactggaaa agaactagct
    20041 agagtaagag ctgatgggat ttatagacca gacgtaaatc aagcttataa tactttagga
    20101 aatgttggct atcatgtatc tttcaatatg cgtaattttc ctaataagaa agtctatgta
    20161 atgatgcggg caacgaatga tccagaaggg aacactaaag gcggagcaca agattttcat
    20221 gataaacgct ggtacttgaa tattcctaaa cgataaaaat agcccctc
//
LOCUS       DAHEYX010000031        19890 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHEYX010000031 DAHEYX010000000
VERSION     DAHEYX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.031"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            10..306
                     /locus_tag="MB219_000774"
     CDS             10..306
                     /locus_tag="MB219_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCC8426509.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(950..1903)
                     /locus_tag="MB219_000775"
     CDS             complement(950..1903)
                     /locus_tag="MB219_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8426510.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1926..2681)
                     /locus_tag="MB219_000776"
     CDS             complement(1926..2681)
                     /locus_tag="MB219_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8426511.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2678..3637)
                     /locus_tag="MB219_000777"
     CDS             complement(2678..3637)
                     /locus_tag="MB219_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCC8426512.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3630..4580)
                     /locus_tag="MB219_000778"
     CDS             complement(3630..4580)
                     /locus_tag="MB219_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCC8426513.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4730..5182
                     /locus_tag="MB219_000779"
     CDS             4730..5182
                     /locus_tag="MB219_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCC8426514.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5271..7538)
                     /gene="gshAB"
                     /locus_tag="MB219_000780"
     CDS             complement(5271..7538)
                     /gene="gshAB"
                     /locus_tag="MB219_000780"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCC8426515.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7725..8723)
                     /gene="ftsY"
                     /locus_tag="MB219_000781"
     CDS             complement(7725..8723)
                     /gene="ftsY"
                     /locus_tag="MB219_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCC8426516.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8734..9543)
                     /locus_tag="MB219_000782"
     CDS             complement(8734..9543)
                     /locus_tag="MB219_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8426517.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9552..13133)
                     /gene="smc"
                     /locus_tag="MB219_000783"
     CDS             complement(9552..13133)
                     /gene="smc"
                     /locus_tag="MB219_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCC8426518.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13153..13839)
                     /locus_tag="MB219_000784"
     CDS             complement(13153..13839)
                     /locus_tag="MB219_000784"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCC8426519.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14187..15968)
                     /locus_tag="MB219_000785"
     CDS             complement(14187..15968)
                     /locus_tag="MB219_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8426520.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15994..16908)
                     /locus_tag="MB219_000786"
     CDS             complement(15994..16908)
                     /locus_tag="MB219_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426521.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16935..17897)
                     /locus_tag="MB219_000787"
     CDS             complement(16935..17897)
                     /locus_tag="MB219_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426522.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17897..18841)
                     /locus_tag="MB219_000788"
     CDS             complement(17897..18841)
                     /locus_tag="MB219_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426523.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18842..19846)
                     /locus_tag="MB219_000789"
     CDS             complement(18842..19846)
                     /locus_tag="MB219_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426524.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5522 a         4270 c         3538 g         6560 t
ORIGIN      
        1 ggaatcacta tgaaggaata tataaaaaac atctatttta tcgaagaaac acaaaatatc
       61 gagggttctt atattgaagt aaaaacactc tttgtcaatg aagataaaac caaagcattg
      121 gatatttata aaaaattagc ttcaaaaaaa acgaatagtt ttggattgat attaagcgag
      181 tataaaatca aagcagaaga gagttatttc tatcaattat taaaacggtg gagtaagctt
      241 cccgccgatt tttatcgaaa aatgcagatc atcaactatc aaccgcttgc tgagacacat
      301 gcctaaatag aggttgtgaa atcagtgctt tgagctaata ccgaaggtca gctgattgaa
      361 cattgtttag aagagattgt gacaactgcg atttgctttg agtaataaga acgctggaca
      421 gaaaatagct tctcatattt tctagtctag tgggcttatt gccgagaagc agcagatgga
      481 acatcgttta gaagagattg tgacaactgc gatttgcttt gagtaataag aacgctggac
      541 agaaaatagc ttctcatatt ttctagtcta gtggacttat tgccgagaag cagcagatgg
      601 aacatcgttt aaagaagaga tcggtacaaa ataaaaatat ctaaatttaa gttttatcta
      661 agtttcgctt tgtacctttg attcaaatca aaggaggcga tcttgatgct gaaaaaatgg
      721 atcacactat tttttgccac tccccattct agaatcaacc cgtatgatgt agataatgaa
      781 ttcacgttga aaaaagattt ctcaaataaa taaaaaccat ccgtaaaatc atcaatacga
      841 ttttacggat ggtttttgat aacttctttg aggataggct cttgctattt aagaatgtaa
      901 gaaacattat tgttacatag tatgagagtt taatcctctg ttatttatgc tatttaaatg
      961 cttgattcac atcttcgatc atcagtttca ttgattctaa accgcccccg cttaaatacc
     1021 agacatctgg ttcaagggag atgatctgct gatttttacc agcattcgtt tgtgctacca
     1081 gttcattggc agaaatatca tctttgctgt cgtctccacc aatagctttt gttctgtcta
     1141 ctacaaataa gatatccgga tttttttcca gcacatattc ataagaaaca ctttgaccgt
     1201 gagtggatgc ttcgatttga tcatctgctt gttcaaagcc aaataaatca tggatgaaac
     1261 cgaatctaga accagatccg taggctgata attgcccttc attcacaagt gtgactagtg
     1321 cttttttgtc agtagcttct gctttctttt tcgtctgatc gattgcacta gataattcct
     1381 ctaattgagt tttagcttct tcttgcttac caaagatctc ccctaaagca gtgacatttt
     1441 gctggaaaga ttcccatgga tttttcgcat ctaaagaaag gaaaatggtc ggagcaattt
     1501 gacttagatc tttttgaaaa tctctttgtc ggccagaaat gatgattaga tctggctgaa
     1561 gctgattaat tttttctaag tctggttctt ttattccacc tgctgattct acttttttat
     1621 aggatgaaag atactcagga aggctgcttg tagctgctcc aactacctta tctccaacac
     1681 caagagcatc catcgtatct aaagaaccat tgtcgaatac gacgactttt tctggattat
     1741 ttggcacctc gatttggtct ccattactat ctgttatcgt caatgtagtt tcagtttgtg
     1801 tgctgctttt ttttgtgtct gcctgactgg tgttgttgct atttccacat gctgctaaac
     1861 caaatgatgc tagaatcaag atacctacta ctaatttttt catctcttct ctcctaatgt
     1921 tagttttaga aataaagaca gaatctgcgg ccttcaatct cacaaatccg tatgtccatt
     1981 tcgtataatt catccaatac atcttttttt atcatttctt gggtcgttcc ttgcgtaaat
     2041 aactgtccat ctttcattgc aacaatttcg tctgcataac ttgcagcaaa gttgatatca
     2101 tgaagaacaa taacaattgt tttcccgatt tcttgtacta aattttttaa aatctgcatg
     2161 atttgaaccg caaaattcat atctagattg ttcaaaggtt catccagtaa aatataatcc
     2221 gtgtcttgcg ctagcaccat tgcaatatac gcacgttgga gttgaccgcc tgaaagagtg
     2281 tcaatcgatt gatccgctat ttcttctaac ccaaggtgtt ccagtgattc ctgaacgatt
     2341 tcctcgtctt ttgtcgtcaa ttttcctttc gtataaggaa atcgtccaaa ttctactaat
     2401 tctttcacgg tgatttggac atttatttca ttcgattgtc gtaggatcga taattttttg
     2461 gctaattcat tggatttcca agcctttact tcgttatggt ctaaatagat ttcacctgta
     2521 tcttttggta tcaaacgact gatcatcgac aacaccgttg actttccagc accatttgga
     2581 ccgataaaag cagtcaattt cttttcagga atcgttaact gtacatcttt aaccactgat
     2641 tttccatggt acagtttatt gaccttttgt aaatcaatca taggatactc ttcctttcgc
     2701 gaatcaattt tgtgacaaaa taaattcctc cgacaaattc aacgacgaca ctcactgttg
     2761 tattccagcc gaaaatctgc tctactgcga gctgtcctcc tgtcagaaac agcatagcta
     2821 ccagaaagct tccgataaat agctgaccat gacgataagt agaaaagaca cgataactga
     2881 tatttgcgac aataaaccct aaaaaagtaa ctggacccac aagagccgtt gatgtccctg
     2941 tcaaaagact gacacaacat aaaatcaaaa attcaaaacg atctgtgtct atcccgaggt
     3001 ttactgcttg ttcatttccg agatggaaaa cgtctagcac atgtcgatat cgaaataaca
     3061 ggtaccagca gccgataatg atcaaagaag caggcaaaag atactgactc tggatattgc
     3121 caaagcttgc aaataatctg ccttgaagca aatcatattc attcggatcc atgatgactt
     3181 gtaaaaaggt actgacacta ccaaaaagcg tacctaaaat catccctgac atcagtaata
     3241 aaaaaagatt cgctctcatt tttttcaaaa gaaagaaaaa taacaataga ctcagcccga
     3301 ccatcagccc aacgttcaat aagaaaaaaa ctgtacctgt ctgctgaagc atcgtcactc
     3361 ctccgataaa gaagaaaagg accgtttgga caaggacata taacgactca agtcccaata
     3421 ctgaaggagt caaaaactgg ttatgcgtaa ttgtctgaaa gctgattgtg gcactgctga
     3481 cagctagcga gacaaggata aatgcaaaaa ttttcttact cctcaatgca catgcaaaat
     3541 gccagtttcc atatgtatgg atcgtcaagt acattcctgt aacaaataaa acaagaacaa
     3601 gcataccaat aaaaatagat ggcttttttt taagcatatc gtcgtcccct tatcagaaga
     3661 taaaggaaga tagccccacc gatgattcca acgatcacac tgactggtat ttcataagga
     3721 aaaatcaaca cgcgagcgac aagatcgcaa aatactaaga acaaactacc tgctaaagca
     3781 gttacgccaa ttgttttgtg cacttgatct ccatatattc tagagacaag attcggaatg
     3841 atcactccga taaaaggaat gcttcctacc atgatcaaca tgactgcact agccaatgat
     3901 actaagccaa tgcctaaaaa ctgcatcttc tggtaatcta ttcccagatt tactgcaaac
     3961 gattcgccca gaccaatgat cgtaaatcgg taagcaaata gccaaaccaa gagcaacaat
     4021 ggaacaacag catacaataa ctcataattt cctcgtatca cagtcgcaaa attcccttga
     4081 agccatgaag tcatattttg taccaactgg aattgataag caaaaaaagt agcaactgaa
     4141 ccaatgatat tccctaacat gactccaatc aacggaagca tcaattgatt tttctggagg
     4201 aacaatcgcg taagggataa aaaaagcaat gttcctgcaa aagcaaagca aaatgctgtc
     4261 aaggatcgga caatcaagga tgcaccaggt aggaaaatca tagccactaa aatacctaaa
     4321 cgcgcactat ccatcgtgcc cgcagtcgtt ggtgaaacaa atttattttg ggtcaaatgt
     4381 tgcatgatca acccacaaat actactggtc gctcccacaa ggatcaaaca aatcgttcgt
     4441 ggcaaacgag tcactagcaa gatttgtaag ctttgcccat ctaactggag cagttctgac
     4501 aattgtacat tcgatactcc gatgaacaat gaaatactac ttaaaataac taataatgct
     4561 actagtcgtc cgtagcgcat ccaatccacc tttctaattg agaatgattt tcattcttat
     4621 tttaagtgaa tcgttgaaca aaagcaaccg ctttttcata tgttttaggt attattggga
     4681 atatgctata atcgtaagaa agacaaagag aagaaggagg gtatttttta tggcacaaga
     4741 tgatgtaaaa agccatttgg ataagggact ttatggtaca cctttagtga accctgaaga
     4801 acagcataaa tatttaggga cattcaggga acgctgctat cttagtatga ctgttgctga
     4861 aatgatccaa ccaaaaaaca aagaaaactt tttaaaagaa ctcaaaaaac atccagatgc
     4921 ttccatactc ttgaatgggg ctctacccct ttctgtccaa aaccaatata tccagctagc
     4981 aacgaaaaac aatagccgtt tcactgtagt caacgattac gtcactgcaa atcctgagga
     5041 attcggattg ctcttaacag caaaagaagc agtaaatgaa cctgtgattg atatcgaaaa
     5101 aaaataccca gaagaaaact cttcgccagc tgaaaaagaa aaaccaaaaa agaaaaaaag
     5161 cttctgggat cgtttatttt aaaacgaaaa aacacataaa tttttacatg caaaaaaagc
     5221 ttttgatcag attgacatca aataggacaa tctgtcaaaa gctttttctt ttaaaatact
     5281 tcaggatata acaatttcaa tacattcatc gtcaatcttc tgccttttcc tgcaaaagga
     5341 taaacatgca tatgcatggc tggattgaaa tttgcttcga tgatcccata tgctttttta
     5401 tcagttgtag gatcgatttc tttatctggt ataatcaaat cgataccact aatcttagct
     5461 cctagtgctt ctaccgcaga aacggcgatt tttttgtaag cttcagaaaa ttcatcagtc
     5521 atatcgatcg aatcaccacc tgtactgata tttgagttct cccttagata aacaatttga
     5581 ttggcttggg gaactgattc gatcgtcaac ccttgttctt tcaacatcaa ttgttctaat
     5641 tcacctaatt ggatcaattc taaaggtgca cgatgattcg ttccacgcaa agggtcacta
     5701 tttttttctt caacaagttc tttcactgtt cggatactgt caccaataac attagcaggg
     5761 actcgcaaca tgatggcttg gacttctccg tcgatcacaa agaaacgata ttccgttccc
     5821 ggcataaatt cttctactaa aaccgaactg tcttcacgaa aagcaatggc taaacctgct
     5881 tgataatctt ccagactagc tccttccttg aaaatcgtga tacctaaccc gtaattggtt
     5941 gatttaggtt tgatcacaaa tgcctgctcg gcaaatctcg gataagcttt taccgcttct
     6001 tccatggatg aaaattctgc gcctcctggt acccggaacc cagcttcttt taagactttt
     6061 ttcgttactg ttttgttttc catgataagt gggacgatat agctgtcctt acttgtcatg
     6121 tttgcatttt tcacatattc tacatggtct tggaattgca gacgcaaaaa ttgatccgac
     6181 tcatctaata ctttgacttg gacacctttt tggatggcat cgaacataaa tatctgagta
     6241 gaaagttcca tttcgcgaaa accggccaac tgatagggac gttcatggct ttctttataa
     6301 tattgatgtc ccaatgccac agcaacttct tgctgactcg atagctgtgc ttcttgatag
     6361 agtttcgctg ctaacgtttg ttcaggatga tccatttgag tcaatgcttc ctctactaaa
     6421 taaagagaat ccaaccaatc caattcaacc agcatttgct tcattccttc aagaatttct
     6481 ctaccttctt gatgaaattc tgtttgatct gatggttgtt ctagtgctac tttgttattt
     6541 cgagtcgtac cttctgctac ccattggtca cagtcttctt tttcttctgt ccataacatg
     6601 tacatcagga ataattgtaa gaaccgaact tgctctggat tgatacccaa tcttgcataa
     6661 ggattcaaat cgatattccg caattcaata taacgaacac ctgttgttgc taaatctgcg
     6721 actttctttc cgcctcggaa acgaagcggt gtataaaatt ctttctcctc cgaaagtttc
     6781 ccttcttcga tcattcgctc gatatctgct aaatactgtt tcatagaagc ataggaaact
     6841 ttcacatttg gatgattcgt atagcccagc gcactgttgc gaatactgcg aacaggttct
     6901 tgcggatgtg attcatctaa aaagtagttc ttctcactca ttggtgaagc accgaataaa
     6961 taagtgatca tccagcgata gcgcataaag tttctcgctg ttttcaaata aagttctgtt
     7021 ttgaattcgg aaaaatcttg aaattcttcc tgatggctga acaatgttct tagcaactca
     7081 tcgccaaatt caaaattgaa atgaataccg cttaccatct gttttcgctt cccgtattct
     7141 tttgctaaat aacgtcgata caagacatct tcgaaatttt ttaattttgc aataatgatc
     7201 tcttcgtctt tttccggtaa ggcaggtggc atacttaatg gccagatcat ctcttctttc
     7261 ggtatagaac gagcagtcac atcataaaca gcagcgagat actgaaataa ttcgggaata
     7321 gaatcggtaa caggggtgat catctctgtt tgtgtttcgc taaaatctgt ttggatatag
     7381 gggtgatagg atcgatcgcc aaagattgct ggatgatcgg tttttgctag atttcctgca
     7441 agatcaaccc gctgtccttc ccgttctata ccaaatcgag cttggtcgat aaaagggcgc
     7501 gcattgacat gcaataataa ttgcttaaaa ttcatcatta agtgacctcc aattcatatg
     7561 aatctaatta taatcaaaaa gtctaagaaa aagaacgaaa ttgtttgaga accgcttaca
     7621 aataagtata aaaatgaagg catcaaaaac acagctatgt taaaaacaat cattcaaaca
     7681 aggagctgca acttttgtca cagctcctgc tttttagaca tgggttattc ttcttttagt
     7741 aatcctttga acaatccaac gacaaaatca tttggatcaa acggctccaa gtcatcgatt
     7801 ccttcaccaa gaccgaccaa tttcactggt aaatgcaatt cattacggat agcaagtacg
     7861 ataccgccct ttgctgtacc atctaatttt gtcaaaacaa gaccggtcac atctgttgtt
     7921 tctttgaatt gttttgcttg aaccatcgcg ttttgaccag tggttgcatc taatacaaga
     7981 agtgtttctt ggggtgcttc tggtaattcc cgctggatta ctcgttttat tttttctaat
     8041 tcgttcatca agttcacttt attttgcagt cggcctgcag tatcaactag taaaacatct
     8101 gcctgctctg tctttgctct ttccaaagca tcgaatacga cagctgctgg atcgcctcct
     8161 gcattgccac gaacaacttc aacaccagcg cgttcgcccc agacaactaa ctggtcgatt
     8221 gctccagcgc ggaaggtatc tgctgctgcc atcaatactt ttttaccttc gtttttgaac
     8281 tgatgggcaa gtttaccaat acttgttgtt ttccctacac cattaacacc gacaaacaaa
     8341 ataactgtta gtccgtcttc ttggatattt agttcattta cttcattgat tccttccgct
     8401 tcatagagat ccacaagttt ctcgatgatc gcattttgga cttctgccga ttttttcgca
     8461 tttctgagtt tcacttcttg acgtaatgct tcggtaattc gtatcgctgt ttcaaaacct
     8521 acatctgcac cgataagtgt ttcttctaat tcttcaaaaa agtcttcatc gactgttcga
     8581 aaattggcaa acaactcatt catccgttca ccaaatgttt tacgcgtctt ttctaaaccc
     8641 ttgtcgtact tctcctgtac ttcaacttct tctgttttct cttcgccagt aaaagcacgc
     8701 ttgattttat caaaaaatcc catttgactc cccctactct aatacaaatt tttggatcac
     8761 tttggctacg ccattttctt cattgctggc tgtcacgaaa tctgctgctt ctttgacgaa
     8821 aggcacagca ttctccatcg ctacgcctag tccagcatat tggatcatcg gtaaatcatt
     8881 ttcctcatca ccgatcgcca tgatttcttc ttgtttcaaa ccaagatctt ttgccaataa
     8941 agaaatacca taagcttttg taacaccttt tggcataaat tccaaaagca tacttcttgt
     9001 tttgatgatc tcatacattt catggaattc tgctggtatt tctttgattt taggatctaa
     9061 ttcttcttgg tcataagcga ttactgcctt attaagcaca aattcatctg taatatcgct
     9121 tatatccgct ggttggaatt gcagcagctt attcaacaca ttgtacaagg attgcttcct
     9181 tggggcacta ggcagctgta aaaccacatt gtcagacaaa acatctagtg gcagatccaa
     9241 ttgctgagcc agtttgtata agcgttggat atctgatact tccatcactt tcttttccat
     9301 gatttcacca gtatcatttt tttgtaccaa accgccgttg aaagtgatgc tgtagtcacc
     9361 ttcatctacc agtcctaatt cttgcagata atgcgccatc gcagctaagg ggcgtcctgt
     9421 acatagaacg atcttgaccc cttgctgttt tgcttgtgct aatgcttgtt tattctcatc
     9481 tgaaatcagc ttttgttcat ttaataatgt accgtccaaa tcgattgcta ctaatttgat
     9541 cattttcgtt cctacacttt cgtttcgttg attttcgcct cttcattgat atcttccatc
     9601 cgtacagaaa cgatttttga tacaccggat tcctgcatcg tcactccgta cagtacatct
     9661 gctgcgacca tcgttccttt gcggtgtgtg actacgataa attgtgtgtc atcttggaaa
     9721 tcgctcaagt aacgaccaaa acgtttcacg ttggcttcat ctaacgctgc ttctacctca
     9781 tctaagatac aaaacggaac aggacagaca cggataatgg aaaagagcaa cgcgatggct
     9841 gtaagtgctc gttctcctcc agaaagcaaa cttagacttt gcagtttttt gccaggaggt
     9901 tgtacttcaa tctcaatacc tgttttcagc aaatcagttg gttccgtcag tactaattca
     9961 gctttaccgc cgccaaacat atttggaaag acaattttga attcttgacg gatagcttca
    10021 aatacttctt tgaatcttgt ccgcacttca tcatccatct catccatcgt ttcaaacaat
    10081 tggtttttcg ctgctaacag atcatctcgc tgtgttgcta agaatgtatg tctttcactg
    10141 acttgttcgt attgttcgat tgaatttaag tttactggtc ccagtttttc aatctgttct
    10201 tttaaggatg aaacctttgt tctactgtct tcgatatcgg ttgtttcttg atagtcttgg
    10261 gaagctttct caaatgtcag ctgatattct gactgcagat aggtcaaatg attatctaaa
    10321 atcatttcag ctcggtcttt ttggacttct aatttggatt gttccgacag tcgtgctttt
    10381 tgctcacgat tacgctctgc caagactgct tcaagcttat cgatctcctt ttgccttcta
    10441 tctcgctgct ctttggcttt tgccaactcg gcttttaatg tttctcgctg agcagataat
    10501 tcgttaattt gtttttccaa actttcttct gtgacttcat gatcagaaaa gtctgctgtc
    10561 aatgtcgcca actgcttttc tatcgcttct tgacgttcgg ttgcctctgc tttttggact
    10621 cgggcaccgc ggagctggat ctgcagatga ttaaactgct ctttcaagac agcaagatct
    10681 gcttgttctt gtgctttttg actttgtact tgtgctcttc tggcttccat ctggtcgctt
    10741 tcttgactta acgatttgat ttcttcgtcg attttttgtc gttgcgattc aagatctgtt
    10801 tgtttgtctt tcaattcgtt cgtttgcttt tcgtaatctt cgataaactg ctgcagttca
    10861 cgtgtctcaa aagttgagat ttgcttttct ttttcaaaac gttcaagttc atttatgatg
    10921 ttttgtagct gatttgtcgc ttcttgttct tcaaaacgta attgttcgcc tcgtgtacgc
    10981 aaaacttctt gttcttcggc taaacgagct gtttcttgtt gcagctcctg aacttttttt
    11041 tcttgtgctt ggagttgttt atctgcttct tcaaattcac tagtcaactg tttcaattct
    11101 tgattttgta caaacaaact gccttggttt cctcgtttat tcgctccacc tgtcatggat
    11161 cctccggcat tcatcacatc accttctaag gaaaccactc gatattgata gcggatcgtt
    11221 tgcgcgattg cattggcact agttaagtct ttggctaaaa ggatcgttcc tagcaggttt
    11281 tgaaccacag tttggatttg atcgggataa ctgacttgtt cactggcaat ccctataaac
    11341 ccttctacag ctgctgcttg tgtcaatata tgagccggta attgtcttgg tttgattgtc
    11401 gtcagtggaa ggaatgttgc tcttcctcct ctttgctgtt tcaaatacgt gatggcttgc
    11461 cgagcatctt tttcattttc aacgatgaca tgctgggctg ccccacctaa agctgtttcg
    11521 atagctaacg tgaaatctgc aggaacatcg atcagttcag ccactgctcc gacaataccc
    11581 gaaagctgct gtttatgctg caagaccaat cgaacccctt ggtaaaaacc aaaatagttt
    11641 tcttggattt cttgaaggct tttcttacga gcacgtagct gctggacttg attcattaat
    11701 tgatacattt taggctgttc ttttgctaat gcttcttgga actttttccc gttttgttgg
    11761 atcatttcta acttctcttt tgtttcagtt aaagatgatt ttagctgcgc ctgcttttgt
    11821 gtcaattctt ctttttgtaa gatcaatcgg tctactgatg cttctacttc cgattgcttg
    11881 gctaacgtct gcttgctttt tgctgtttct tgtatatatt gacgttctag atacttcagt
    11941 tcgtttccta cagctgcttg ttcttgcatc aaatcaacat attgatctct taattctgcc
    12001 aataattctt tcgttgattt ttggtatttt tctaattctt gctgtgtttt tatgagattt
    12061 gcttcagctt tttggatctc tgtttctttt tctgctgctg ctttcatcaa gctttcttga
    12121 agcttttcaa aatgtttaac ctttttttgt gcttctgcta atgaagtttg atattcttgc
    12181 gagctttttt gtgtatgctt tgtccgctcc tgcaaaacat ctttttgtcc ttctgtctgc
    12241 ttcagttttt ccgaaagatc taataatact tgctgatttt tttcgatcag acgatcggct
    12301 tgcgcatttt ctttacgctg cttcgccaaa atggattctt gttcttggat cgactcagat
    12361 agtttgccta attctagatt gaatttcgca agctgtgctt gtttgttgtc ccaatctttt
    12421 tttgctgtct tgatttcagc aaccatcaaa gagacatcgg tttgagtcag tgtttctttt
    12481 agccgtaaaa actcctttgc tgcctcactc tgggcggcca aaggagttaa ctgttcttcc
    12541 aattcgtgga tgatgtcttg gacgcggctg aggttgtctt ctgtttcaaa aagcttctgt
    12601 tctgctttct ttttccgctg cttgtatttc aagacaccag ctgcttcttc aaagatccca
    12661 cgacggtctt ccggtttact actgaaaatc gcttctactt ttccttgaga aatgattgag
    12721 aacgattctt ttcctaatcc agaatctaag aacaattctt ggatatcttt taatctgcag
    12781 ctttgtttat tgatgaaaaa ctcgctttcc ccagtccgtc gataacggcg ggttacactg
    12841 atctcattgt actccaatgg cagataatga tcactattgt ctaaaatcac tgtgacttct
    12901 gcaatattca gctgcttacg ggtatcggaa ccggcaaaga tgatatccgg catttttccg
    12961 cctcgcagac ttttagcaga ttgttctcct aatacccagc ggatagcctc tgtaatattg
    13021 ctttttccgc tgccatttgg tccaaccact gctgtgacgc tattttcaaa atcaatgact
    13081 gtcttgtctg caaatgattt aaaaccggct atttcaattc gtttcaaata caaagctttc
    13141 cctcacttac ttttacagtg ctgccaatgc attttccgcc gcagcttgtt ctgctaattt
    13201 tttcgatttc ccttgtccag tgccaatcaa ttgatcatct acataaactt cgatccaaaa
    13261 tactcgttca tgagctggac cttcttcatt gatcaatcgg tattcgattg atacatcacc
    13321 tgatttttgc aagacttctt gtagttttgt tttatgatcc atctcatgtg aaaaagcacc
    13381 tgctttgact ttaggaaaaa tcactttttc gatgaatgca tgggtcgtgt cgaatccctg
    13441 gtcaagatat aacgcgccta aaaaagcttc gaataggtcg caaagcaatg ctggtcgcgt
    13501 acgtcctcct gaattctctt ctcccttgcc caacaaaaca tattgatcaa agtgacattc
    13561 tttggcaaat ttcgacaagc tgtcttcgcg aacgatcgca gctcgtgttt ttgtcaattt
    13621 tccttctggc aaatgaggat ataaacggaa taaatactga gaaaccatca gttctaaaac
    13681 ggcatctcct aaaaattcaa gtcgctcatt atccgataat tgcagattgc gatgctcatt
    13741 cacataggat gaatgggtaa aagcttgttc aagcaaattt agatcgtgaa aaacgatgtt
    13801 atagttttct tttagttctt ttgtcagctg gttgtccatt ttccacattc cttttatgga
    13861 tattttttcg atacttcctt attatactgg ttttttgtca aatttgaaat ggttcggtca
    13921 aaaggtaagt aaagctttaa taatcctagt gattgacgag aagttgtgaa aaattcgttt
    13981 agtttcgagt atgaagaaaa aattttgtga aaatggcttt ccatattttt gcataaattt
    14041 gacttaatac cgagaaactg gactttgaac accgtttatt ggagaagtca tgaaatcagc
    14101 gccttgacct gaatattaga gaaataaaca gaaaaatatc aaaaaaccga aaaaggacat
    14161 cgcccttttt cggtcatttt tagtgattat gaacgtgaat ctgctgtaac agaaacattt
    14221 gcccatgggt ttggtacatc ataagaccaa tcccagtctt tgacacggtc gttgactggc
    14281 aatacttcat tacggaacaa cgttggaatc gcaaatgctt cttcaaatgc atattgttgc
    14341 catttttgat atgcttcttt ttggtactct tcatcaaatg ctttgcttga actgatatcg
    14401 ttgatcaatt ttgtattttc ttctgattca taacgagtgt agttgaacgc agcatttggt
    14461 ccccatagtc cacttggatt tggatcagtt cctgtattcc aagcagcttg gtaaacatca
    14521 atttcaggat catcgttttc taatttatca tagaaagaat tgaattcgat cagacgacct
    14581 gttgtgtatt ggacatccaa accaatttct ttccattgtt ggatatagta atctgccaat
    14641 ggttgcgctg tttccccacc ggacatcgaa gcaaagttga ttgttagttt atttccatct
    14701 ttgtcttctc ttaaaccatc gtcatccaca tctttgtagc cagcatcatc taatagtttt
    14761 ttcgctttgt ctagatcttg tgtgaatcct ttcatctcat catcatggta tgactggaat
    14821 actggaggaa tcaatgaagt cgcattgctt ctcaaacctg catagaaacg ttcacctact
    14881 gcattattat ctaatgcata gcccattgct tgacgtaatg atttatcagc catttttgaa
    14941 tctggattgt attccacact ctttgtatct gcattccatt tacctaactt gaatcctaga
    15001 taagtataag aagtttgttc acggcctaag tttgtatagc cagcaatttc tttataggta
    15061 tcgtaagtat ctgttggcat ttggaaaatc atatcatata atttagagtt caatgcttca
    15121 gttgcacttg ccatcggtac agatttcatc gtgatctttt tcaatttagg cgtaccattc
    15181 cagtagtgct cgtttggcaa gtattctact gattctcctg caacgatatt actcatgtaa
    15241 taagggccaa aagtcactgg atttttacgt acttgatcgc ttgattccat atctttgatt
    15301 gctacacctt ctaacgtatg ttttggtgct gcatatgacc agataccacc acccgattga
    15361 agcatgcttg ggtttacttc tttatattcg atttcaactg ttttgtcgtc tatttttttg
    15421 attccggaga ttgtgtcggc acttccatca tgatattctt ccatacctac gatgttacgg
    15481 aaagtgtcgt cataacggat accagtgtaa tctttattcc caatgatttc gtaagggaaa
    15541 atcacatcgt ccgccgttac atcttcacca tctgaccatt tgacgttgtc ttttactttg
    15601 attgtcgctt ttttcgtatc ttcatctaga tcaagcgttg cagctccgtc gtctgtgatt
    15661 ctgaaatcgt catcgtttgc aaatagcggt tcatgagaag gagccataaa agctgcatca
    15721 tatgaatctt ggtaaaactc ccactggaac aatcctttaa actgactgtc cgttgcgacc
    15781 gcagcttcta attcgccgtc ttcgattgca tcatcattat tttttgttgc aattggaaaa
    15841 gttaattcct ctttgttatt gccgttgcct ccagagttgt ttccagaggt atctgtacct
    15901 ccgcccccgc atgctgcaag aacgacagca gacatcagcg ctgcaaatcc aaaaatcgtt
    15961 ttcttcattc ctatttcccc cttcgcttct atactagcct aaccgttggc gcgcatctgc
    16021 agaacgcttg aatgcttgtc caacataatt gatactcaac atcaaaacta aaataaggat
    16081 tgatgcgggt aaccagatcc attgccgatt cactaagact tctccgttac ttgcataacc
    16141 gatcaatgta cctaaactag gtacatttgc cggcaagccg aaacctaaga aagtcaaagt
    16201 tgtctcgata ccgatgtttg ccgcaaagtt catcgtcaaa ttagtaatga ttaatgaact
    16261 tagattcggc atgatttcgc ggaacatgat tttaaaatca ttggttccca ttgtttttga
    16321 ggcactgaca taatctcttc ttgcttcaga taatgtcttg cttcggaata atcgggcttt
    16381 agctacccaa taaaatgcac tcatgatgaa tataaaggac cagacattgt atctggaaat
    16441 aatcgatacg aagacgataa tgatcagcat gatcggtaga atcatgataa aatcaacgat
    16501 ccgcatcaag atgttgtcaa tgatccctcc gtagtagcca gagatgatcc ctaatccaac
    16561 cccgatgatc gaagtaagga tcgtgatcgc aaatccgatc gtaacagagt ttctagcacc
    16621 tatgatcaat tgtcctaata catcacgtcc gccttcatct gctcccagaa tgaacttttg
    16681 tccttgtgtc gaaactgttc ctggtgcagc gtatttgtcg aagatactaa catacatcac
    16741 tttgtcttga tcggtcagca tcgcaccgat aaagatcact aataataaaa ccactagcaa
    16801 acctaatgaa aaaatagcaa ttttatcttt tttaaattct cgagcaatca ttcgtatccc
    16861 cataggagga atactttctt gaactgctgt attgtttttt tcactcatcg atccattctc
    16921 ctttctgatt ggttttattg aatccgaata cgcgggtcaa cgatactcat gatgatatct
    16981 gaaagtaatg tccccactag cgtcgcgatt cctaaaatca atactaagga tgtgatcacg
    17041 gtatagtccc gttggccgat ggaatcaata aatagttttc cgactcccgg ataaccaaaa
    17101 atcttttcga taactacaga accagcaatc aacgaagtga tttcataccc catttgagaa
    17161 gccatcggca acgctgcatt tcggaaaata tgtcgactga atactttatt cgtcggtacc
    17221 cctttggaac gggctgttct gacaaaatcg agcgatttcg aatcgattac ttcacttcgc
    17281 agatattgga ttgtgacggc agtcgccaaa aatgcttggg taattgatgg taacagaata
    17341 tatttcaaac ggtctatcca ttgagtgata aatccacctt gcaaacctag tgtttgtgat
    17401 ccacttgttg ggaaccagcc taaacgataa ccaaaaacaa atagcatgat caagccgaag
    17461 ataaataaag gaacagcaaa actaaagaag ttgtagatta cgacaatctt atccaagatt
    17521 gaattttgat accggccagc aagcatcccc agaggcacag caatcaaata agtaaggaca
    17581 actgtcaaga tcgatagata taaagtgttg atgatccgtt cgccgatcaa gtcagcaaca
    17641 gagcgtttgt ataagaaact ttgaccaaaa tcgccttgca aagcatttcc gatccaacgg
    17701 aagtactgga cataccatgg gtcattcaag ccagctgctt gtcgcatctg ttcgatcact
    17761 gccggatcct gatttggatt gatcaaaccg gtaaatggat cccccggcat cgcttgggct
    17821 aataagaaga tcagaacact taaaataata acttgcggga tcatcaataa aacacgtcgc
    17881 agaatcgttt tccacattag cttgcacctc catctttcaa cgccactttg tgtgtagttg
    17941 tgatcgtacg caaatcgtag actcgaccag tcgcatcata gtattctttt tggttttgga
    18001 tatactcttg ttctactctt cgtcgatttt ctttgtggct ttccctattt tctacatcga
    18061 tttgcggaat agctgaaagc agacgtttcg tatagatatg tcgcgggtca ttataaatgt
    18121 cttctcttgt accgatttct acaaatcgac ctttgtacat aattgcgata ttgtcgcaca
    18181 tgtgttttac tacacctaaa tcatgtgaaa tgaataaata gctgaggccg tattcttgct
    18241 ggattcgttt catgaaattc aatacttgcg cttgaacaga tagatccaga gcagatacag
    18301 gttcatcggc tacgatcagt tttggattgg tagcaacagc acgggctact cctagacgtt
    18361 gacgttgtcc gcctgaaaat tcatgaggat atttatatag cgcatcttcc ggcatcccaa
    18421 cgatatctag cagtcctttt acctttttct tttcttcttg atcgcttaac cgttcaaaat
    18481 ttcggatggg ttcagcaatg atatccaaca ctcgcttttt aggattcaaa ctggacattg
    18541 aatcttggaa gatcatctgc acatccttgt tataacccat ttttttgcgg ttgcttcgct
    18601 tcgttacatc ttttccttca tacatgattt gtccggctgt taccttttct aatcccacaa
    18661 cggcttttcc cgtagtagat tttccagagc cagactctcc cactaaacca tatgtctttc
    18721 ctttttcgat aataaaatcc acaccatcta cagcatatac atgatccgtt actcgattga
    18781 agaatccact gcgaataggg taatgaactt tcaggtcttt gattgtgatt aattctgtca
    18841 tctacacctc tcctttttcg tctctaaagt ggaaatgctt atagcaggag caacgtacca
    18901 agtggttcgg tgcgacttca tgaaggatag gctcttcttc atgatcgctt gctggtatcc
    18961 atggaatacg tggtgcaaac cgacaacctt tacgaggcat gtttttcaga gaaggaacga
    19021 caccttcgat cacatggagt tctgcatcat gatttccttc ttgtggtata gagttcagta
    19081 aagagcgtgt ataaggatgc ttaggatgat caaacagttc ttttacacct gctacttcaa
    19141 cgaactgtcc gccatacatc actgccactc gatcagccat ttctgctacg actcctaagt
    19201 catgagtgat caagatgatc cctgtttttg tttcttcctg aaggtctttc agtaaatcaa
    19261 gaatctgtgc ttggatggtt acatctaggg ctgtcgtcgg ttcatctgca atgacgattg
    19321 gaggtttgca ggcgattgcg attgcgatga tgacacgttg tctcataccg cctgatagtt
    19381 catggggata ttggcgtcca actctcgcag gattaggaat ccctacttgt tctagcaatt
    19441 ctagtgcacg agcttgacgc tgttctgcat tcatctttgt gtgataggaa agtccttctt
    19501 tgatctggtc ttcgattctc atgagaggat ttaatgcaga caacggatct tggaaaatca
    19561 ttccgatatc atttccgcgg attttattat agagcgtttc gttcaggtcg attaaattca
    19621 gatcgttata taaaatctca cctgtaattt ttgtgttatt aggatcaagt aatcccataa
    19681 tcgtagtagc caaggtactt ttcccgcacc cagattcccc aacgattgct aaaatctcgt
    19741 ttttgtctaa agtaaatgaa acgtcatcta ctgcatcgta atagtcatct ttgagtcgga
    19801 atcctgtatg taggttctga acttctaata attggttatc ggacaatcga atacccctct
    19861 ctgcttccta aatggaagtt tttcttaata
//
LOCUS       DAHEYX010000032        19555 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHEYX010000032 DAHEYX010000000
VERSION     DAHEYX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.032"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            105..1148
                     /locus_tag="MB219_000790"
     CDS             105..1148
                     /locus_tag="MB219_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCC8426525.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="MB219_000791"
     CDS             1321..1545
                     /locus_tag="MB219_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426526.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="MB219_000792"
     CDS             1548..1805
                     /locus_tag="MB219_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426527.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2022..2897
                     /locus_tag="MB219_000793"
     CDS             2022..2897
                     /locus_tag="MB219_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426528.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2907..3701
                     /locus_tag="MB219_000794"
     CDS             2907..3701
                     /locus_tag="MB219_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426529.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3730..4848
                     /locus_tag="MB219_000795"
     CDS             3730..4848
                     /locus_tag="MB219_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426530.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4881..5201
                     /locus_tag="MB219_000796"
     CDS             4881..5201
                     /locus_tag="MB219_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426531.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5456..6145
                     /locus_tag="MB219_000797"
     CDS             5456..6145
                     /locus_tag="MB219_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HCC8426532.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6146..6349
                     /locus_tag="MB219_000798"
     CDS             6146..6349
                     /locus_tag="MB219_000798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426533.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6369..7997
                     /locus_tag="MB219_000799"
     CDS             6369..7997
                     /locus_tag="MB219_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426534.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8048..8419
                     /locus_tag="MB219_000800"
     CDS             8048..8419
                     /locus_tag="MB219_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426535.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8504..8704
                     /locus_tag="MB219_000801"
     CDS             8504..8704
                     /locus_tag="MB219_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426536.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8838..10064
                     /locus_tag="MB219_000802"
     CDS             8838..10064
                     /locus_tag="MB219_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426537.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10914..12238
                     /locus_tag="MB219_000803"
                     /pseudo
     CDS             10914..12238
                     /locus_tag="MB219_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            12231..12578
                     /locus_tag="MB219_000804"
     CDS             12231..12578
                     /locus_tag="MB219_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426538.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            12893..13750
                     /locus_tag="MB219_000805"
     CDS             12893..13750
                     /locus_tag="MB219_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCC8426539.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            13770..14330
                     /locus_tag="MB219_000806"
     CDS             13770..14330
                     /locus_tag="MB219_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HCC8426540.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            15303..16070
                     /locus_tag="MB219_000807"
     CDS             15303..16070
                     /locus_tag="MB219_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HCC8426541.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            16096..16641
                     /locus_tag="MB219_000808"
     CDS             16096..16641
                     /locus_tag="MB219_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426542.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            16642..17208
                     /locus_tag="MB219_000809"
     CDS             16642..17208
                     /locus_tag="MB219_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426543.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            17223..17471
                     /locus_tag="MB219_000810"
     CDS             17223..17471
                     /locus_tag="MB219_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426544.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            17490..17729
                     /locus_tag="MB219_000811"
     CDS             17490..17729
                     /locus_tag="MB219_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426545.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            17746..18330
                     /locus_tag="MB219_000812"
     CDS             17746..18330
                     /locus_tag="MB219_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426546.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            18437..18718
                     /locus_tag="MB219_000813"
     CDS             18437..18718
                     /locus_tag="MB219_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCC8426547.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            18708..19049
                     /locus_tag="MB219_000814"
     CDS             18708..19049
                     /locus_tag="MB219_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCC8426548.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         7501 a         2480 c         3937 g         5637 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagcta cttttttcaa
     1621 ataagtacac agccaaagag ttggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaaggaatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggat ttcaagctga caatgattct aaaaaagttg
     1801 aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg gaaaggtaga ttacaaagga
     1861 aaacactttt aaataaactt cgcaaaagca tcaggttatg agtggctagg gctatacgaa
     1921 tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca ataggggggg aacaaaattt
     1981 ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata tatgactact aacaaaaggt
     2041 ggtcagagct agtacaaaat acagttaaaa aagtcatgcc agatggatat catttcaact
     2101 atatacaatg ctcacaaaca actgaaagta tttatttttc aatattaaat gagaatagac
     2161 tctattattt cagggtaagt tcacaccata ggaaaaagag agaagataat gttacgacat
     2221 ttttatcggg aaaatacaat ggcttcaaag agttaagtga agccattggt gagtatctaa
     2281 aaagtaatag atttgtcaga atttctgaca aacatatatt gatcttgtta ctttatttga
     2341 ataatagctt caaattgttt tggagagacg acactttcta ctatattgag aacggcatgg
     2401 atatttattc tggtatcaac ggcatattaa aacattatat taaggcgtta cttcgatatg
     2461 gggttcttta tcaagaagat ttaagtaacc aaattaagat tagtaattct ggaaagtcat
     2521 tactggcaaa atataaaaaa ttgctttctc ctatctacaa aggaatagat atggaagctg
     2581 taaattctga aactttattt aatttagttg ttgatgaaaa gtttgcgaat gaatttttta
     2641 aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga aaatgcaata aaagcttatg
     2701 tagataataa taaatcacga ttatacaact ggtttattcc caaacggttg cgaggaaaaa
     2761 tcaaaattgg cgatcaagtg aaagtaatta atctaaatca tactaagatc gttaccgtaa
     2821 cggagttgta tatagctgaa aaagaagata ttacaaaaat gaaaccagtt attaaaatac
     2881 tagataacaa agagtgagtg gaatatatgg tagcaaaaaa gaatactcta aaagaagctt
     2941 ctgaaaaata tcaagatatg aaaagacgag gattaatacc aaaggacaaa aaaggtatat
     3001 ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt gttggacgat
     3061 atacagcaaa aggttatagc aggcgcttaa ggatcactgt tgaatatgga acgaatgaat
     3121 cagctacaga gctgaagcaa aggtacttag atacacgaag agaagcttca aaaaaatatg
     3181 atgatttcaa gagcagaggt ctaattccta agaagaaaaa ggggatttgg cgtgagtttt
     3241 tcaaagaaga agatagcaat cggaataagg gagacttcat tggacgatac acagcaaaag
     3301 gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt tcatgggaat
     3361 tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta ttggagttgt
     3421 tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa ggttttatga
     3481 tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg aaaaaagcaa
     3541 gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga aaccggagaa
     3601 ctgaatcgag tgagattgaa gattactatg atgcctttac acacgatctg ggagcctttg
     3661 tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa gccggagatg
     3721 agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa aattaaaaga
     3781 cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac gatcaaatat
     3841 cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt gggctatgat
     3901 aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag tacctccggt
     3961 agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata tgatttcaac
     4021 gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta tttgaaagca
     4081 aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt aagaccgtat
     4141 gaaaatttaa aaacgtatat gttctttttg gttaattttg aaggaacagc caaagaagga
     4201 acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa tcccaaaaca
     4261 gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga ttattatgat
     4321 attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg attactcaaa
     4381 gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa gctttataaa
     4441 aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga aggagaaaca
     4501 agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt agcaggcgta
     4561 tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata tgggctatta
     4621 aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga agagctgtat
     4681 aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt cgaaaaagct
     4741 ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc taaagatgaa
     4801 gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat actctttgga
     4861 aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta ggtgttatta
     4921 ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa aaagttgcac
     4981 gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta aaaggggatc
     5041 agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt gattataaga
     5101 aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa tgggaaggta
     5161 ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa aacagttctg
     5221 actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc attcaaaaat
     5281 aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa gggtgttgtg
     5341 atagacgatg atttcaagtt tgattttgac tagatttttt tgcaatgttt aagagtaaac
     5401 aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg aatgtatgag
     5461 aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa aaagtcgaga
     5521 acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata gtgaagttcc
     5581 aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt ttcatgtcag
     5641 ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt ctatctctca
     5701 atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg cttttgattt
     5761 tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg agaagctaaa
     5821 aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt tctactccga
     5881 agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt ctcataagtt
     5941 atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta aacttgaaga
     6001 agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg aatttgacga
     6061 acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg aacttagcta
     6121 tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata cgatcgcaga
     6181 tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc acgtaaacga
     6241 tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat taaaaaggtt
     6301 tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag gagagagtgt
     6361 gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa aaacagttag
     6421 atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag gtacagatac
     6481 taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa cccaaggcat
     6541 tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat atgatcaaaa
     6601 agtatagacg agataattca aaagttgaag acgttttatt caacggtacg gcaaaacatg
     6661 atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt aatcagacag
     6721 atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata agtagcgaaa
     6781 caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat aaaaaattga
     6841 tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta ttcaaaacag
     6901 gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca gcattttatc
     6961 cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt gaaattaaaa
     7021 agatgaaaga actattaggt ataggatttg taaaacccaa ttcaaaagtc actattgctt
     7081 cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat tcagatatag
     7141 atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg atgggtattg
     7201 atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat attgtgtatt
     7261 tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa attgactatt
     7321 tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt aaaaatgcta
     7381 aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca agtattagaa
     7441 aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg agtaaagaaa
     7501 acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt ttgaagcaag
     7561 agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt gaaaataaag
     7621 tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa actgaagagc
     7681 cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta gtgggaggac
     7741 atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg tttgatgtaa
     7801 gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt tttctatata
     7861 cagattactt gaatcatggt ctatattatc gtgttatgag tgaaatttct aataaagata
     7921 agatatttta tttggataat catgtgaata tgaacttaac aattaaacgt atttacgatc
     7981 ttttatcact tatataaaga gagaagtgat aaataggaca caaaatttgg aaagtaggta
     8041 agtcactatg agagaactaa ctttatcttc taaggaatta gaaaagaaaa ctgctttatt
     8101 aaacgaaata tttagtgctg attgtataat ccaaagttta aaagaaagtt cattttcaaa
     8161 actaacagat caggaaaaaa tttcttatat gctaagttta gataatctag gaactaaata
     8221 tgatgaatca gcaagtagcg ataagctttt gattgaatta gtatcaagtc ttagtagttt
     8281 tggaaaaatg aaaagattta atgtgatatt agaaattttt gcttggttaa tcccttcatt
     8341 tatttggttt ttttatattt tcatagttga atctgagtta atagccaagg tctctcttgg
     8401 actgttaatt ccgatataaa taggttcagc aatttacttt aataacttgt atacacgtat
     8461 tcaaataaaa aagcagttat tacatgaagg attaagtggt aatatgttaa aaagaattgc
     8521 atgggataaa gataataaaa cagatattga gcttaaagaa tataaataca attatttctc
     8581 gagatgtata gatagaaagt tactgtttaa aaaagaagga ttagaattta gttttgatga
     8641 agatgaattt gaaaaattag tgatggactg gcgtaagaat ttactggata cggtacaatt
     8701 ctaaaagcaa ttgaggtcga aattccgata cttgaatcat gatctatatt atcgtgttat
     8761 gagtgaaatt tctaataaag ataagatatt tcatttggac agtcatgtga atatgagctt
     8821 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgctaaaat
     8881 tgacaacaat aactatgaca aaaaaaatag aagtatatgt tgatggatca caagaagaaa
     8941 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
     9001 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
     9061 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
     9121 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
     9181 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
     9241 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattccgcaa
     9301 atatgaaagc tagtaaagat tttggcaatg attttatcgg aattgtgacc ggacaaagaa
     9361 cgcctagaat atataaaaat agaaacaaat atggattact accttttaga ggtcgagata
     9421 ttaaaatcca taaagatgat aagttttata tttatttacc taaaggcttt aggtttaaag
     9481 tgaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
     9541 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
     9601 ttaatctaac aatttctttt gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg
     9661 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttaattaat gataaatctg
     9721 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
     9781 aatcaaaaga acaaagccga ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat
     9841 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taattttgtt aaaacttata
     9901 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
     9961 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    10021 tagtaaacaa aggaaaaaga tatgggatag aagtcagaag ttagtaaatt aaacaatcgt
    10081 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    10141 tgtgtgacca gacggaagga cgaatgttgt tgaggtttta gaaccatata aaacagcatg
    10201 tttctcaaat ttttaggata agggaaattg ctagcagttt gttttagaac catataaaac
    10261 agcatgtttc tcaaatgagt actttaaatt aattgatggc gttcctgctt tagaaccata
    10321 taaaacaaca tggatttaaa acaagaagta agtcagttct ccttttactt tagtaaggaa
    10381 aaataaagta ttctcaataa aacttaaaat aagcaaacct ttgttttaat agctaatcga
    10441 agtaaaaccc aaagccatat gaacccctga atcagcgaaa gtttatgatg atttggatta
    10501 ctaatagttt aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa
    10561 atagtgaggg aaaatccact cactgaaggg taaactccgt caagttatgt gaccagacgg
    10621 aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatagttct caaattgttt
    10681 aaaatgattg tcaagttaaa aaaatctggg atcatataaa atagtatagt tctcaaatat
    10741 gattagaaac cctattaaag agcaatatta agccaactta cttaaatcgt ttaattaaga
    10801 acaataatct cttttagtca gacttttagt ctgacttttt tgtatttcca aaatttattc
    10861 tttacaaaaa cgaacatacg tttgtataat tgcttgtgaa tgggggaata aaaatgtttg
    10921 attacagtag agaaccggtg aatgactatt ttttaatcga catgaagtct ttctatgcta
    10981 gcgtggaatg tattgaacga aatttagacc caatgactac accgttaatt gtcatgagta
    11041 gatcggacaa cactggttca ggtctgactc tagcttcttc acctactgca aagaaaaaat
    11101 atcatattac taatgttagt agagctactg atttacctta tccattacca aaagaacttc
    11161 atattgttcc accacgaatg aacttatata taaaaaaaat atggaagtta atcagatttt
    11221 tagaaagtat gtcgcagatg aagatttatt gatttattcg attgatgaat caattttaaa
    11281 agtgacaaaa tccttaaatt tatttactag ttcaggaaca agaagcgaga gaagaagaga
    11341 attagctaaa ataatcaaag atgagattca gaaagaaaca ggtctaatag caacagtcgg
    11401 aataggagat aacccattat tagctaaact tgcactggac aatgaagcga agagtagtaa
    11461 agaatttata gctgagtgga cttatgagac tgtacctgaa aaaatatgga aaataaacga
    11521 gatgactgac ttttggggca ttggatcacg catgaaaaaa agattgaatc atatgggaat
    11581 tatgagtata gaagatttag caaattggaa tccctacaca attaaaagtc agttaggtgt
    11641 aatcggactc cagttatatt tccataccaa tggaattgat cgtacggata ttgctattcc
    11701 accagaacca accaaagaaa agtcttatgg gaattcacaa gttctaccaa aagattatac
    11761 gaggagagaa gaagtagaac tagtagtcaa ggagatggca gagcaagtag ccattcgaat
    11821 tagacaacac aattgcaaaa ctggttgtgt acgcttagca attggtacgt caattttgga
    11881 agaaaaatca ggtttctcgc accagatgaa aataccaatt acagataata cgaaagaatt
    11941 acaaaattat tgtctttttt tattcaataa gttttatgag gggcaggaag tacgacatat
    12001 aggagtaact tatagcagat tagtatacac agattcccta caattagatt tatttagtga
    12061 tccaagaaaa aagattgctg aagaaaattt ggataggatt attgataaga ttagacaaaa
    12121 atatggattt acagctatta tacatgcttc atctatgtta gaaggtgcaa gaagtattac
    12181 gagaagcact ttaataggag gacacgcagg aggattagga ggtttgcata atgattgaat
    12241 ataacgatag aggacgttta aaatggtcgg gattttattt gtctgatcat actgaaagaa
    12301 tcaatgcaga caatgtacaa cgtgctaatc ataatttggc aaaagaacaa atgacgactg
    12361 aagagattgc gaaaatactg agaactgctg tcataaaaaa tagacccgtc tgcattcaaa
    12421 aagaggaacg aaacgctgaa ggttactatc cagatgatgt agtaggaaaa attattggat
    12481 atgacgagct tggtgtattt gtggaagaag aaaaagttca ctatgatgaa attaggaatg
    12541 tttctttctc ggaattatta aaatggagtc agatatgaac attattaaaa aatacgaatt
    12601 cggttatatt acgtatgaac agctaataga ggaattatgg ggatatggtc aacaattaat
    12661 taatgaagtg gggattgatt gcttttgttt ctatattgaa tccggagcag gataccataa
    12721 gaacagatac tatatatttc catatccacc aaaatagtct cttagatatt tttaaatttt
    12781 ctccaaatac tttcaattat tgtgaaatat ttccctttaa ttaatcaaat tttttataaa
    12841 agtaatatat aaaataaatt gcttgaagta ttatatttag atatcataat ttatggaaga
    12901 aaaattaatt aaaaaggatg agattataat ggggttactg gttccgagag ctaatactta
    12961 tactatacct ttaacgaaac gccaattaat tgctaaaaga tatcaaagga ttactagagc
    13021 catcaataga gagttttgga attctgaaag tgatactgct cacagtttat acgtaggttc
    13081 ttatgggaga ggaacggcaa ttagtactag tgatatagat ataatagttg aattaccgat
    13141 ggcagaattt gacagattca aaaattattt atcaaatggc ccgtctaagt tattacaagt
    13201 tataaaaaac gcatttcagg aaatactacc aaatagtgat attagagctg atggacaagt
    13261 tgttaaaata aatttccatg atggaatcaa atttgaaata gttccagctt ttaatgaaaa
    13321 agattattgg ggtgagtcaa aagggtttat ttatccagat tcaaacatgg gagggaactg
    13381 gaaagctaca aatcccaaaa aagagcaaga agccatgaaa ttaaagaata caaaaagtaa
    13441 taatttatta tatgctacgt gtaaacattt tcgacatgta cgagatacag aatttacgag
    13501 ttatcatctt tcaggaatag taattgacag tttcgtatat gaggctatgg gaaattggaa
    13561 attcgttgaa aataacagtg ggggtcaaaa cattagttct gtatcttatg agacagcttt
    13621 attagaatac tataactcac ataaagttat gggcggttta aatttatact ctcctggaag
    13681 caatcaattt gttaattctg atagtagtat catatgttta gaaaaagtat taaaaaaaat
    13741 agctttataa agttttatgg aggaacaaca tgggagacga aaaaaagcgc ttggaagttc
    13801 aattacgtga agcatatgga cgagtaacgt atacttacac cactcattta aagcaagccg
    13861 ataatattat ttctgacaat aaaattttaa ggtgtcttca aattggtttg tcagccctta
    13921 ctactggagg ggtacttagt accattatta ctgataatag tgagttaacc tttgttgctg
    13981 gattatgttc aacaatttct cttggattaa acttatactt taagaatttt gatcttgctg
    14041 aagaagctaa aaatcatcaa attgttgccg ataaattatg gttgatcaga gaagaatacg
    14101 taagcttact aacagattta aatcgtttaa gtgttgaaga tattgtgaaa aaaagagatc
    14161 aattaatgaa cgaaactcat gaagcatata aaaatgcccc taaaacaaat agtaaaagct
    14221 atgaatcagc tcagaaagca ttaaaatctg aagaagagca attttttaca gaagaggaat
    14281 tggataagat gttaccagag catcttagaa aaaagagttc taaatattga actctttttt
    14341 tatgcttaaa attctcttgt aatttttcta ttcatctata tttaagaaga aatatgatga
    14401 tagaaacatc tcctaaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
    14461 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaagccgtc ctagccattg
    14521 cctagtccga catcgctaga ttagtgcatc tcgccataaa agttttctaa agccaccgca
    14581 agccgtccta gccattgcct agtccgacat cgctagatta gtgcatctcg ccataaaagt
    14641 tttctaaagc caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct
    14701 catctcacca tgaacgtttc ctaaaaccac cgcaaaccgt cctagccatc gcctagtgcc
    14761 tagtccgatt ttgcttgatc ggttccattt ctgtttattt agtgggtcaa caccgtaatc
    14821 ttaaatagcc atcgcctatt taagattact aggtctgctt tgaaggtaaa aagtctttca
    14881 ttcttcaaga cttttaggct ttcaagctac gcctgaaagc attgaaattc atcgctcttt
    14941 atttggtttt gccatttata attccttacg cccattatct ttttcgcctg tcttgccttg
    15001 tcagaagtgt ttagagctta tctcatcttt ggattaatac aagtggcgta aatttttttc
    15061 taaacgcaag cgttacgaaa ataatttact taaacacttg tattaatccg gcagataaga
    15121 acgcattcta atcactcccg acaaggcaag acaggcgaaa aagataaata aatgaacgaa
    15181 aggaagaaac ataaaatgca aaaccaaata gcgatgaaga aaaaaccacc tccctctaaa
    15241 acaatccaaa caaaaaagaa ttttgaaaaa ccaaaaatca aaaacagaaa gaagcgttca
    15301 acatgagaga tagaatttta gaagttaatc aagaaatggt aaacaagtta acaaatatca
    15361 gtgggaacta tgaaaagtac gatggtactt tacttgataa ttatattttt cacgatacac
    15421 aaaatatcag aattggcaga agtaaaccga gagcgttcat tctcatcaaa gagaaatatt
    15481 tgaatgaatg gaatagcacc tatgaaatgg ttatgactga taactttgaa aaggtattgg
    15541 atttcagaaa tgctttgttg ggttctgata tgggaaatag cagtcttgaa tctattgaag
    15601 ttgaaaacaa gaccgagaat gaaggtgaag gattgattaa gaatgtcctg aaaatcgaac
    15661 aaaaatatat cagtgaagat agaagttttt atagagtcac aagctcggtt ggtctagcaa
    15721 actttgctac taacttaatt ggaaacaatg ctagtgaatc tttgctagtg atgtgtctga
    15781 atacaaagaa tgaggtcatt gcttatagcg aagttttcaa aggtggaatt aatcaatcag
    15841 tagcgatacc aagagagatt tttcaactag ctttgctaaa caattcagca aaaatcgcag
    15901 tatcacataa tcatccgagc ggatatgaat ttccaagtga aaatgacaaa agttttacta
    15961 cgaatttgaa aaacgcagga aaaacaattg gtattgaatt gttagaccat atcatcgtaa
    16021 caagtaaaaa cacaagctat tactcatttc aagaaaatgg ttctttataa aaattagaga
    16081 aaagaggaaa acaagatggg cgaaagaaca caagcgttaa ttacagtcaa atatggtaac
    16141 agcaaaacga aagtaagcgt gtcaattcat tatcagtggg gatatggtcg attcatgttg
    16201 atggacgttt tgaatcaagg aatatattta atcaataaag catatgactt acgagatgaa
    16261 tcaagagaag aattagcaaa aggaatcttt gatagaagta caggagttat caattttgtt
    16321 acctatgaat ctggagtgag tgcttatgat gaaacagggt ttggtgggcg ctatgaacct
    16381 tatcaagaaa cgattgaaga tgtgatgaag ttttcagata acaatgatgg ctatgctcat
    16441 ttagagatgg tgttcgatga aaatggattt agtctagatg ataaaaccag attgcgattg
    16501 tttgattttg agggtcaaca gataggacta aacaagtttt gtgaaagcgg aaaagagttt
    16561 gctacgaaag attttattaa gggatacaga ttattgctag aaaattatgg aataaaccta
    16621 gaagaagaga gcgtgaaata agtgtttgaa aaaagaaaag accagttccc taatttagaa
    16681 gcgaaaagtc aaaaagtgat tgatggtgaa atgattgaat tattccaaaa aagttatgga
    16741 accatagaaa ctaccgcaag ttgcaaatac tactttcaag gaaagagaaa cacgtttaaa
    16801 ggctttgccg attctgatga agaattggca aagaaaatca ttgattggct tgtcagcagt
    16861 gaacacgtag caagagaaga taccgttaga acaacgattg agggtgaagt cctaacaggt
    16921 actagatgtg tttgttgtaa tcatgaattt gtagaagatg aaggttgcta ccgaatcaat
    16981 gaatatggtg attttatttg ccagagttgc cacgagatag aatttccaac cgaaagaagt
    17041 tggtttgaat acatgctaca agattatgag atggaaacag aaaatggtga agtagttttt
    17101 tgtaccaaag aggaattgag taagagaagc gatgatgaaa ttgaagattg gtgtgtgtgg
    17161 ttagtaggga cagatgattg cgttgcatat tacacaacag cagaatagga gtgtgaatca
    17221 gcatggaagg aatggatttt atagatcatg aagatttgat tgattttggt tatacatgga
    17281 aaggaatggt aggtattagc agaagtcttg ctaatgcctt ttacgaaaga aattatgcag
    17341 tctatgtcct ttatgatgac aatacagaaa gccttgttga tgaagagtat aaattggatt
    17401 tagagaatgt tttatatggg atagaaaaag aagatttagc aaaatatatt ttttcatggt
    17461 tgggtcaata aacgaaagag ggtagcaaca tgacaaaatt caaaagtaaa ggtatgcaat
    17521 ggtgttctta ttattatgtt gggaatgatg aagagctttt gagaaaagcc aaacaaaaag
    17581 gttatccaga taagatgtta aaacagattg gctattacat gccagaagag agaatagaaa
    17641 aagaatcttg taaggttctt tttgatagtg aacagctatt agttttgtta actcaaaata
    17701 aattgatttg tcaatttgaa aataattaat atggggtgaa gaaccatgaa tgagaataag
    17761 atggtattaa gtgaatacgc aagattgaga accattaatt ggttgaatgg tatgcaaagt
    17821 agtggcgctt ttgatggatt tgatgaggag aatatggact ttgaaagttt atcgaatgag
    17881 gatattagat attattttat cgaatggtat tttacagaac cagatgaaga ggtggaagat
    17941 gaaccagaat taattttaga acgtcaaaga attttattca gtgaacaaga tagtaagagg
    18001 ttgtatggtt tatatgactt acaactgaaa ggattaaaag cgcagtatgt tgctgatagt
    18061 tacgatgata tggtagaaca gggggcagat agggttgtca cttattcaga agatgatgaa
    18121 gaggaatggg aaaactattc agatgatgag atatgcttta atgatgggta tatgcctatt
    18181 gaaatcaccg aagaagaata tgataaagtg tcggatagcg atgagattgg tatgtgtacg
    18241 gttgtaagtg gattggatat agatgtccaa agagtagagg ttgcatacca gaagatgaaa
    18301 gaacgtataa aaagtaaagt agaatcttga tttgattgga ttgtttgcaa agtagctaat
    18361 taagagtata atataatcat aaatgtagtt aatggatata ataatgagta tattatactc
    18421 aaaagaggtg aacgaaatga aggtaaaaaa tccaaaagaa aaaagcagaa ttcaagtcgg
    18481 aatagacaag gatttaaaag aaaacgcaga aatgatattg gaagagctag ggttaaatcc
    18541 aacaacagca atcacaatcc tttataagca agtggtcgct agaggtgagt ttccagtaga
    18601 gataaagcta agcgaagagg aaaagcaagg aatcagattg caacagctaa ctatggatat
    18661 gccagttgat gttctagata cagatgaaaa gttagaagag tggttcaatg aagcctaatg
    18721 agatagtaac ggtctatgtt gcatttacag ataaaaatgg tggtaaaaga agacctattt
    18781 tggtggttag tgataaagaa gatagagtag aattttttgg aatcacaagt caatatgaga
    18841 ataaatcaga ccaaataaag agagtgtatt ttcctattgc agaatgggaa aaagtaggtc
    18901 taaagaagca atcatggatt gacgtagggt ctttaaaggc tattcccaaa agccgagaga
    18961 acatatcatt taaaaaggtt ggtatgctat cagctattga tacgaaaaag ctaaataggt
    19021 ttatcgagag attacaaaga aaagtctaac aaggcttttc tttttttgtt tatatgtata
    19081 ttaagatata cgtttatata aacgtattta tgtatattaa aatacacgta aatatatacg
    19141 tgattataaa aattaagaaa atattaagta ttgttaaata gtgataatta gtaatatacg
    19201 aattataaga tacattttat atttaatgta tgtaaataaa taaaatgact gacggatatt
    19261 gaatagtaat aaataacaat aattcagacg gatacagtga gtgaagtagc ttgctacgaa
    19321 cgaacaggca gaatgaaacg gtagtttcat tagagcaagt cgcttgcgac gtagcgttac
    19381 cactgatgta actggcaagt tacatagaga gagtttgctt gcaaacgaac gttacccact
    19441 tgaatagaaa acaaaaataa catattagtg aattttgaat aggagccaca actcaaccaa
    19501 ccgcttgcgg tgggtgagtc ccacggagga aaagacgaac gaacggtcgc gtagc
//
LOCUS       DAHEYX010000033        19477 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHEYX010000033 DAHEYX010000000
VERSION     DAHEYX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.033"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1230
                     /locus_tag="MB219_000815"
     CDS             <1..1230
                     /locus_tag="MB219_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCC8426549.1"
                     /translation="ITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNKEAITIYGSSA
                     AKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDLMTPERMEDYH
                     RILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEVEKLAEYQISM
                     ISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAFANATDLDGQL
                     LPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTLQDPFIQEMRR
                     YYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDSFMIKRYVGKA
                     KDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEFSVPEQDWTIP
                     FNQFPTYPNSRALLYKN"
     gene            complement(1319..2266)
                     /locus_tag="MB219_000816"
     CDS             complement(1319..2266)
                     /locus_tag="MB219_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCC8426550.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2660..3952
                     /locus_tag="MB219_000817"
     CDS             2660..3952
                     /locus_tag="MB219_000817"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCC8426551.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            3967..5736
                     /gene="aspS"
                     /locus_tag="MB219_000818"
     CDS             3967..5736
                     /gene="aspS"
                     /locus_tag="MB219_000818"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCC8426552.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5845..6720
                     /locus_tag="MB219_000819"
     CDS             5845..6720
                     /locus_tag="MB219_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCC8426553.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            6986..8869
                     /locus_tag="MB219_000820"
     CDS             6986..8869
                     /locus_tag="MB219_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCC8426554.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(8944..9228)
                     /locus_tag="MB219_000821"
     CDS             complement(8944..9228)
                     /locus_tag="MB219_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCC8426555.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9218..9952)
                     /locus_tag="MB219_000822"
     CDS             complement(9218..9952)
                     /locus_tag="MB219_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCC8426556.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10081..10704
                     /locus_tag="MB219_000823"
     CDS             10081..10704
                     /locus_tag="MB219_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCC8426557.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            10896..12017
                     /locus_tag="MB219_000824"
     CDS             10896..12017
                     /locus_tag="MB219_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCC8426558.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12024..15152
                     /locus_tag="MB219_000825"
     CDS             12024..15152
                     /locus_tag="MB219_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCC8426559.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15171..18365
                     /locus_tag="MB219_000826"
     CDS             15171..18365
                     /locus_tag="MB219_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCC8426560.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILPLYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18511..19218)
                     /locus_tag="MB219_000827"
     CDS             complement(18511..19218)
                     /locus_tag="MB219_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCC8426561.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6493 a         3452 c         4125 g         5407 t
ORIGIN      
        1 atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga gttacgccat
       61 gaagcggacg ctcccgtcgt caaagcaaag attttcaata aagaagctat cacgatctac
      121 ggatcatcag cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa aggtgctatg
      181 ccaaaattag tattgaagac actttttggt caaggtggag tacagacttt ggatggagct
      241 gctcatcacc atcggaaaaa tatttttatg gatctaatga caccggaaag aatggaagat
      301 tatcatcgta tcttagataa gaatctgaca caagcacttg aagcacagca tggtcaattt
      361 gaactatttg atttaagtaa gatggttttc tttacatcta tttgcgagtg ggcaggtatc
      421 aatttatcag cgatttctaa agatgaggtc gaaaaattag cagaatatca aatctctatg
      481 atcagcggaa cgtttacttc tccgattgat cacataaaag gtgtcgaaaa tcgcaaaaaa
      541 tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa
      601 gaaaatgtgg cattatatgc ttttgccaat gccacagatt tagatggtca gcttttacct
      661 ctggaagtgg cagctgttga attattgaac atcatccgtc caacggttgc tctaactgtg
      721 tgggcggcat tgatgggaca tgcacttttc agtagaccag atttgtatca gcaattaaaa
      781 aatgactttt cgacgttaca agatccgttt attcaagaaa tgagacgcta ttatcctttc
      841 ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag tagatggtta tcgtatacca
      901 gaaggcagtt gggttatttt agatttatat ggaacagacc atgatgaacg aacagttgaa
      961 gcgccagatt cctttatgat caaacggtat gtagggaaag caaaagatat ttcctataaa
     1021 gaagaatatg agatgattgc acaaggcggg ggaaattttc gacaaatgca tcgctgtgca
     1081 ggagaatgga tcactcttca tagtctacgt gtgttttccg atcagctagt aaataagttt
     1141 gagttctctg tacctgaaca agattggacg attccattca atcagtttcc gacttatccg
     1201 aatagccgag cgctacttta caagaattag attagcataa acttatcgaa aaaaaggaag
     1261 atcaaacagt gctgatcttc cttttttcat attactataa attgataagg tcaattgatc
     1321 aaaatccacg aaatgcttca tcaatggttt gataatcttc attggataaa tgcaaatcta
     1381 aagcttggac attgtcagat acttgctctg gacgcttagc tccaggtata actacagaca
     1441 ctcgtggatt cttcatgtac caagctaaaa caagttgcgc aggagtggct tggtatcttt
     1501 tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt tctccttgga
     1561 aatctggatt tctttttcga ggatctcctt caccaaattg tttttcctca ccaagttcat
     1621 attttcctgt cagcaaaccg gaagcaagag gaaaataggg aacaaaactg attttgtttt
     1681 tttcaagata agggaataat tctttttcag cctgccgatg gatcaaactg tatttatctt
     1741 ctactacatc gacatatcca tctgcattcg cttctttcaa ttgttctaac gtgaagtttg
     1801 acacaccgac tgctcggatt tttcctgctt cttttaattc gtgcaatgta gcaactgatt
     1861 cattttttgg tgtgctttca tccggaaagt ggatataaaa aatgtcgatg taatcagttt
     1921 gcagacgttt tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg
     1981 ttcgcccttt atttggtaca tgagcagctt ttgtagcgat gacgacacgt gagcgatcat
     2041 attctttttc cttcagtacc tcgccaatca gttcttcgga acggccgttt ccgtaagcat
     2101 aagcggtatc gatcaaattg atcccgctat tcaaagctgt acgaactact tgttttcctg
     2161 tctcatcttc caaaccgtca aaaagattat gtcctccaac ggcatttgct cctaatccaa
     2221 gctgttctgc gtagacttgt gaatgtccga ttcttatttc tgccatagct gattcctcct
     2281 ttttgtctag tctaaaacaa aataccaaaa agctgaacta aaacgttttt gtgaggggaa
     2341 atagtcagat atctcttgac tttgagactg gtttttagta ggttataagt aatatcgaat
     2401 gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa
     2461 atgatctaaa gtttcattag cctaaagaca ctctggagat ggacgagtga aaagccgtcc
     2521 cgcgtacgag cgttattcgt aagaggaaag agaaatcttt caaaattagg tggtaccgtg
     2581 tatgaatttc attcaccctt gttcatgatg aacaagggtg atttttttat ttacatagct
     2641 aagaaaggga gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg atattttgcc
     2701 aggcgaatca gaaaaatggc agtttgtcga agaaacagca cgtttattat tcagagatta
     2761 tcagtataat gaaatgcgta caccgatttt tgaacactat gaagtgatat cccgaagtgt
     2821 aggggataca acggatatcg tttcaaaaga gatgtatgat ttttatgaca aaggggaacg
     2881 ccatgtgacg cttcgaccag aaggtacagc accaatcgtt cgttcttttg ttgagcataa
     2941 attgttcggc ccagagtatg cgaaaccgtt caaaactttt tatatgggac caatgtttcg
     3001 ctacgaacgc cctcaaaaag gacggttacg tcaattccat caaatcggag tagaagcatt
     3061 tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg atggcacttg atttcttcaa
     3121 acagttgggg atcacgcaaa tccggctagt catcaattca ttaggtgata aagaaacgcg
     3181 tcaagcctat cgtcaagcat tgatcgacta tcttttacct tttgaatcag agctgagtga
     3241 agactctaaa cgccgccttc atgaaaatcc attacgcgta ttggacagta aagacaaacg
     3301 agatcaaaaa tttgtggcag aagctccatc tatcttagat tatctaagcg agcctgctca
     3361 aaaacatttt gataccgttg tttccatgct ggatgcattg gctgttcctt atgaaatcga
     3421 cagtaatatg gttcgcggac tagattatta cacacatacg atttttgaaa tcatgagtga
     3481 cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc ggacgttatg acaatctagt
     3541 ggaagaacta ggtggtcctt caacacctgg cttcggattt gcaatgggta tcgagcgatt
     3601 actcttgact atggaggcag aggaagtagt tatccctgca tttaatgagt tggatgctta
     3661 cgtggtcgca ctcggagacg aaacaaatat cgaagcattg aaagtggtcc aagcgattcg
     3721 aaactttggt ttttcagctg accgcgactt catgaaccgt aaagcaaaag cgcaatttaa
     3781 gacagcagat aaagcgaatg ccaaattagt attggtgatc ggtgaagatg agcttgctag
     3841 tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc cattagaaga
     3901 gatatatgaa cattttgatg aagtctatga tgaaatgact ttattcgagt aggaggaaaa
     3961 aaataaatgg cacaaagaac gatctattgc ggattagttt ccgaacaaca attagaacaa
     4021 acagttacat taaaaggatg ggtacaaaaa agacgagacc ttgggggagt aatctttatc
     4081 gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat caacaaagaa
     4141 gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt
     4201 agaagacgcg gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt
     4261 gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc catttcaaat tgaagatgat
     4321 caagcaatca gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa
     4381 atgactaaaa acatcaaatt aagaaatgcg acaacaaaag cgattcgtca ttatttagat
     4441 gataatgact ttctcgatat cgaaacacct tacctaggga aatctacacc agaaggcgca
     4501 agagactacc tagttccttc acgtgtccat gctggacatt tctatgcgtt gccgcaatct
     4561 cctcaattgt ttaaacaatt gctgatgaat gctggattcg accgttatta tcaaatcgtt
     4621 cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac gcaagtagat
     4681 attgaaacaa cgttcttaac agcagaagaa atccaaactt acacagaggg attgattgcg
     4741 aaggtaatga aagaagttcg cggtatcgaa gtcacattgc catttcctcg tatgacctat
     4801 gatgaagcaa tggctcgcta cggcagcgac aaaccggata cacgttttga tatggaattg
     4861 atcgatctaa gcgatacagt caaagaagta gaattcaaag tcttccaaat ggctcttgaa
     4921 aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa
     4981 gacatggatc agttaggtca atatgtagga caattcggag ctaaggggct tgcatggctg
     5041 aaagtagaag aagacggatt gaaaggtcca attgctaaat tcatgggaga agcaacagaa
     5101 gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa
     5161 agtgaaatcg tcgctgctgc attaggtgct atccggacac gtttaggaaa agaattagga
     5221 ctaattgatg aatcgaaatt caatttctta tgggtaactg attggccaca atttgaattt
     5281 tcagaagaag aaggtcgata tgtctctgct caccatccat tcacgatgcc aaaagaagaa
     5341 gatatcccgt tattggctga agaccctgca aaagtctatg cagaagctta tgatatcgtc
     5401 ttgaacggtt atgaactagg cggaggttct atccgtattc atactcgtga gcttcaagaa
     5461 aaagtactag aaactctagg ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg
     5521 ttaacagctt tagattacgg attccctcca catggcggta ttgcattagg attggaccgt
     5581 tttgcaatgt tattagccgg agaagataat attcgtgaag tcattgcctt tcctaaaaac
     5641 ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg tttcgccttt acaattgttc
     5701 gaattgaata ttgatgtaac gaatatagaa gaatagccga taaaaaggta aaaaggattt
     5761 gtcgaatacg cttcttttga caaccttgat ttaattgaaa aacactttat aatgaagcca
     5821 taacatataa agcggggctt ttccatgaaa aaaacattcg aaaattgggc ttatcatgat
     5881 tacacgacca agctttcggt atctatcgtt tatgcgttga tcgcatcagt cgcattgaat
     5941 ttcttttatc agccaggaca tatttattca agtggtatta ctggtctagc tcagattttg
     6001 acgactttgt caaaatcact gataggattc gaagtaccgg tatcaacaac tttgtattta
     6061 ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc
     6121 acttttatca cagtcacttt gacctccctt tgtatccata ttttaccgga aacagtccta
     6181 tctaccgatc caatcatctg tgcgttgttt gggggggctg tgatgggact aggtattggg
     6241 ctagcgttga gaaacggcct atcatcgggc ggtctagata tcgtgagtat cacgattcgt
     6301 aaaaaaacag gaaagtcgat tggttctatt tcgatctact tcaatgcatt gatcgttttt
     6361 gttgccggaa ttctatttgg ctggcaatat atgttttaca gtacgttgtc tattttcgtc
     6421 agtggaaaag taactgatgt cgtttatact aaacaaaaga agatgcaggt aatgatcgta
     6481 acgaaacatc cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag agggatcacg
     6541 atcatcaatg aagcagaagg tgcttataag catgataagc agacagtatt gatgacgatc
     6601 gttacacgat acgaactgcc tctacttaga gaagcaatga aagcttctga tccacaagct
     6661 tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt tctatgagga tggcatttag
     6721 atcgagtttg acaaaaattc atgaaatact gctgtagaca gacgttcgtt tgtctgcagt
     6781 ttttttctac catagtagaa tcagcaaaga aaaactgctt ttttatttga aattttctta
     6841 aagttgaaaa aaagcatgtt tattttttta aatttatggg atgatagaaa aaaatagtag
     6901 agaaaggaca cgattctact tgctattttc acttaatacc cgtatattac gaatgaaaaa
     6961 agtttatgaa aagttggaat attgaatgga aaaacaaaaa aggttaaaaa acagccgcta
     7021 catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct tctatcacct
     7081 tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg tcgtatctgg
     7141 ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa tcaatatttg
     7201 gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta tgcttgtgac
     7261 agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc gaggagttgt
     7321 ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt tatcttattt
     7381 cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag tagaaggtca
     7441 aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa
     7501 atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga tgatgatctt
     7561 gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc gtctgttttc
     7621 tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat
     7681 cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt tggcactgat
     7741 tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag gaatgctcct
     7801 agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag caagtttgaa
     7861 tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg gcatttatct
     7921 gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg ccgatcatgt
     7981 tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac tttcttatcg
     8041 atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag tgatgaaagg
     8101 atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag gcacattact
     8161 tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca
     8221 aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa
     8281 ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg agttgtccaa
     8341 ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt cagttatgtt
     8401 ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg gagatgtcgg
     8461 acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag atttgttgaa
     8521 agacactgtt ctgatcggac taggaacgaa tggctcattc tcggaagctc agtttgatag
     8581 tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag taccgacgaa
     8641 gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat acgacaatat
     8701 gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt atgatgatca
     8761 agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg aagccttgct
     8821 aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat agctacaaaa
     8881 aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc gtgtttttaa
     8941 cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg
     9001 ttttcgggtg agttttttga atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa
     9061 tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat atttagcacc
     9121 agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc caccataaaa
     9181 tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag agcatctcac
     9241 gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga gcgactttga
     9301 atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc tcttttccag
     9361 gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc atgacctgaa
     9421 tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt gtttttagta
     9481 gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga cgggcgattg
     9541 ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa ggtggattac
     9601 agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc agatcgatgg
     9661 tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt gccatgtctg
     9721 ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa
     9781 gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta ggtacacggg
     9841 aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa ctttggataa
     9901 tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc atggattccc
     9961 tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag aaccaaggtg
    10021 gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa ggaagagact
    10081 atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt taatggaaat
    10141 gcccgctatg agaataaaga agttttaccg caagatgaga attacatact agttgctccc
    10201 catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa ggaattcagc
    10261 tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt aactcgatcc
    10321 aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac acctgtaaag
    10381 attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag acatgctaca
    10441 gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat cgtgccagct
    10501 gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt gacagtgcgt
    10561 ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg tatggcggag
    10621 atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa ccctgatttc
    10681 aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc ttttgaatct
    10741 actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc aagtctgccc
    10801 tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt
    10861 ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat acagccgatt
    10921 ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt catgtactat
    10981 cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt gccggagact
    11041 tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg
    11101 attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac gatagcagta
    11161 cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg cgaacacagc
    11221 tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt cttccttact
    11281 ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg atcaagcaag
    11341 cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa aaacaagtat
    11401 tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg aaaatcgaag
    11461 taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat gtagcactag
    11521 gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc ggttccccat
    11581 tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa
    11641 caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa gaaataacag
    11701 cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg gagaattaca
    11761 tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag cttcggcaaa
    11821 tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa acaaagcgca
    11881 aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt tttagtgaaa
    11941 tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa
    12001 tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa aattttggtc
    12061 cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt tttttgatca
    12121 gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc gcactttttg
    12181 gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt gctacaccag
    12241 acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat gaaatcgaac
    12301 gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca
    12361 aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta
    12421 cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag ttttctcaaa
    12481 tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc agtgataaag
    12541 agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat gagcgattaa
    12601 aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag ctgcatcttc
    12661 tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt gaagccgttt
    12721 tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag
    12781 ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat cgttttgaaa
    12841 gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag gaattagctg
    12901 ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa tttacgcaac
    12961 gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata gaactgtcac
    13021 aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat gaagagtggc
    13081 aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct gctcaaaaac
    13141 tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg
    13201 cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag ctgtatcaaa
    13261 atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg gatcaggaaa
    13321 aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa aagcagcaag
    13381 aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga gaagaactca
    13441 aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag acagaacaag
    13501 cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc
    13561 gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa caattaaaac
    13621 acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca gaagctgagt
    13681 ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa aataatcttc
    13741 aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac
    13801 aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa
    13861 caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc aagatggcca
    13921 atgcaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa caacaattag
    13981 ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg caaggagaaa
    14041 tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa gaggagatac
    14101 gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag cagaaagcag
    14161 gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt caacatgaac
    14221 aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct aaaaagttag
    14281 aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa cagcaagcac
    14341 aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta gtgacagctg
    14401 accggctgcg tcaattagaa aatatccagc taactgagac attcccagat acagaagaga
    14461 tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa aagatatacg
    14521 gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt tcgatttatc
    14581 acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg caaacattca
    14641 acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt tatcttcaag
    14701 aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg
    14761 agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc aatatctatg
    14821 atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag agttttattg
    14881 cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct ggtggtgtag
    14941 caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa gctttggaaa
    15001 tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc atcattagtc
    15061 atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca gaaggtagcg
    15121 gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact atgaaatgga
    15181 gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc cgagtgttat
    15241 cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt
    15301 atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt ccctattggg
    15361 aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg tcgaaaaatc
    15421 tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca acaagtgagg
    15481 gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct caagcggtgc
    15541 aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat cacccagagc
    15601 tttctatttt gccactttac ttgcgtatcg gttcattggc atcaccgaaa aaaacatata
    15661 tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact tacactgtta
    15721 ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat caaaaaatac
    15781 tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac ggcttacaag
    15841 tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc cgcttcctgt
    15901 tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa aacgatcaag
    15961 gaatacaatt ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa tttggagaag
    16021 attatttgct gcaagcattg aatgattttg attttttctt tatgcattat cagtggggag
    16081 ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat caaacttgga
    16141 aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt
    16201 ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct attgcccctg
    16261 cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc cgaaaagcaa
    16321 aaggaaaaat ccaagctcga gtcgatttcg tctatggcga agtcatctat tcgactgacc
    16381 cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga
    16441 aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact ggttatgaac
    16501 gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta tctgtcttcc
    16561 gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta gatgcgcata
    16621 aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt cgattcgatg
    16681 tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg agaaatgatg
    16741 ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa
    16801 caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga acgatccatg
    16861 tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat gctactttta
    16921 gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac caggcacagc
    16981 ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt cgatggttga
    17041 agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga cttgggaaaa
    17101 cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc ttctctgctt
    17161 tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga aaattcgccc
    17221 cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa ttactggcta
    17281 aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta gctaattatc
    17341 agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa aatagcagta
    17401 cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca ctgagtggga
    17461 caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt atgccaggat
    17521 ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg caaatgattc
    17581 gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag
    17641 agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta gcttacttgc
    17701 gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa aaccggatcg
    17761 ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt ctgtttattg
    17821 aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg gtagctgcca
    17881 aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg actatcttgc
    17941 aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt acgaaaccac
    18001 aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt tttctgattt
    18061 ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc atcttgtatg
    18121 acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat cggatcggac
    18181 aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag gaacgcatgg
    18241 attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac
    18301 agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga gaaatcgagg
    18361 agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa agaagcgatt
    18421 gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc agcttccttg
    18481 ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca ttgtctctaa
    18541 cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc
    18601 tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa catcattgtc
    18661 gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt tctcagctag
    18721 ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga gcatattgcg
    18781 ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat ggtcagtcgg
    18841 cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga gggttttttt
    18901 gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt gaaataagag
    18961 gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc tgatccgatg
    19021 tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt tttcatcact
    19081 atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa gcggaacgac
    19141 tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat caacatcaaa
    19201 cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt tttcacaaat
    19261 ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag gtttttctta
    19321 ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt tcactaaaaa
    19381 tcgtgaaaca cgtcaataag tttgatgaac aaattaatca ttatggtaga agtcgattga
    19441 tttattttaa tgcaatctct gtggaatttt cgaaaga
//
LOCUS       DAHEYX010000034        19418 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHEYX010000034 DAHEYX010000000
VERSION     DAHEYX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.034"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(6..3680)
                     /gene="rpoC"
                     /locus_tag="MB219_000828"
     CDS             complement(6..3680)
                     /gene="rpoC"
                     /locus_tag="MB219_000828"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCC8426562.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3738..7355)
                     /gene="rpoB"
                     /locus_tag="MB219_000829"
     CDS             complement(3738..7355)
                     /gene="rpoB"
                     /locus_tag="MB219_000829"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCC8426563.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8083..9051
                     /locus_tag="MB219_000830"
     CDS             8083..9051
                     /locus_tag="MB219_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8426564.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9069..9974
                     /locus_tag="MB219_000831"
     CDS             9069..9974
                     /locus_tag="MB219_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426565.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9971..10777
                     /locus_tag="MB219_000832"
     CDS             9971..10777
                     /locus_tag="MB219_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8426566.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10913..11902
                     /locus_tag="MB219_000833"
     CDS             10913..11902
                     /locus_tag="MB219_000833"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCC8426567.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12219..12743)
                     /locus_tag="MB219_000834"
     CDS             complement(12219..12743)
                     /locus_tag="MB219_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCC8426568.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13030..14841
                     /gene="scm"
                     /locus_tag="MB219_000835"
     CDS             13030..14841
                     /gene="scm"
                     /locus_tag="MB219_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCC8426569.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(14996..15982)
                     /locus_tag="MB219_000836"
     CDS             complement(14996..15982)
                     /locus_tag="MB219_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCC8426570.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16005..16571)
                     /locus_tag="MB219_000837"
     CDS             complement(16005..16571)
                     /locus_tag="MB219_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCC8426571.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16585..17001)
                     /locus_tag="MB219_000838"
     CDS             complement(16585..17001)
                     /locus_tag="MB219_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCC8426572.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17458..19392)
                     /locus_tag="MB219_000839"
     CDS             complement(17458..19392)
                     /locus_tag="MB219_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCC8426573.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT         5554 a         4240 c         3772 g         5852 t
ORIGIN      
        1 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
       61 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
      121 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
      181 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
      241 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
      301 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
      361 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
      421 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
      481 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
      541 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
      601 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
      661 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
      721 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
      781 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
      841 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
      901 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
      961 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
     1021 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
     1081 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
     1141 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
     1201 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
     1261 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
     1321 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
     1381 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
     1441 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
     1501 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
     1561 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
     1621 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
     1681 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
     1741 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
     1801 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
     1861 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
     1921 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
     1981 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
     2041 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
     2101 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
     2161 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
     2221 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
     2281 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
     2341 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
     2401 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
     2461 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
     2521 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
     2581 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
     2641 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
     2701 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
     2761 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
     2821 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
     2881 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
     2941 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
     3001 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
     3061 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
     3121 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
     3181 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
     3241 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
     3301 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
     3361 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
     3421 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
     3481 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt
     3541 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
     3601 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
     3661 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
     3721 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
     3781 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
     3841 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
     3901 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
     3961 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
     4021 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
     4081 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
     4141 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
     4201 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
     4261 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
     4321 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
     4381 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
     4441 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
     4501 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
     4561 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
     4621 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
     4681 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
     4741 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
     4801 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
     4861 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
     4921 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
     4981 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
     5041 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
     5101 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
     5161 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
     5221 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
     5281 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
     5341 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
     5401 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
     5461 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
     5521 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
     5581 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
     5641 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
     5701 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
     5761 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
     5821 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
     5881 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
     5941 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
     6001 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
     6061 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
     6121 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
     6181 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
     6241 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
     6301 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
     6361 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
     6421 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
     6481 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
     6541 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
     6601 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
     6661 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
     6721 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
     6781 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
     6841 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
     6901 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
     6961 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
     7021 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
     7081 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
     7141 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
     7201 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
     7261 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
     7321 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
     7381 tcaaaaagac tacccgatca attgttacaa taatatttcg aatattattt ttaggtaaca
     7441 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
     7501 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
     7561 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
     7621 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
     7681 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
     7741 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
     7801 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
     7861 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
     7921 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
     7981 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
     8041 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
     8101 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
     8161 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
     8221 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
     8281 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
     8341 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
     8401 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
     8461 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
     8521 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
     8581 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
     8641 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
     8701 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
     8761 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
     8821 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
     8881 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
     8941 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
     9001 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
     9061 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
     9121 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
     9181 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
     9241 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
     9301 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
     9361 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
     9421 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
     9481 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
     9541 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
     9601 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
     9661 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
     9721 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
     9781 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
     9841 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
     9901 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
     9961 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    10021 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    10081 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    10141 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    10201 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    10261 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    10321 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    10381 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    10441 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    10501 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    10561 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    10621 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    10681 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    10741 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    10801 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    10861 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    10921 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    10981 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    11041 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    11101 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    11161 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    11221 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    11281 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    11341 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    11401 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    11461 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    11521 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    11581 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    11641 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    11701 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    11761 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    11821 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    11881 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    11941 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    12001 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    12061 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    12121 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    12181 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    12241 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    12301 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    12361 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    12421 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    12481 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    12541 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    12601 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    12661 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    12721 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    12781 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    12841 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    12901 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    12961 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    13021 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    13081 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    13141 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    13201 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    13261 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    13321 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    13381 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    13441 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    13501 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    13561 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    13621 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    13681 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    13741 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    13801 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    13861 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    13921 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    13981 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    14041 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca
    14101 agcgacagta cagatactac ctctaccaca agtgatagca cggatacatc agcttcaagc
    14161 gacagtacag atactacctc taccacaagt gatagcacgg acacatcagc ttcaagtgac
    14221 agtacagata ctacctctac cacaagtgac agcacggaca cgtcagcttc aagcgacagt
    14281 acagatacta cctctaccac aagtgatagc acggacacat cagcttcaag tgacagtaca
    14341 gatactacct ctaccacaag tgatagcacg gacacgtcag cttcaagcga cagtacggat
    14401 acatctacca caacaagtga tagcacggac acgtcagctt caagtgacag tacagatact
    14461 acctctacca caagtgatag ctcagattca tctaccacta caagtgatag cacggacacg
    14521 tcagcttcaa gtgacagtac agatacatcc tctaccacaa gtgatagctc agattcatca
    14581 acttcaagcg atagtacaga cacgtcgtct accacaagcg atacacgtca tgcaagtgtc
    14641 tccttctcaa aaacaagttc ggataacagg acgaacttta acttaactaa taaaacaagc
    14701 aatgacaatg gaaacaaatc aaaaagagca cttcctaaaa ctggaagtca atcgaataat
    14761 tggataacac tcgcaggcgt tatattactt gtaatcggtt taagaatcac ttttatgagt
    14821 tataagaaat cgaaaaggta aaaaaatatt caagataaaa aagagcaacg tcgctaacgg
    14881 acgttgctct ttatttttgc tttttatcat aaatacaaga actacaattc aatttgtcaa
    14941 aaaacacgta gatttccttc tgagaaattt atttacgtat cgaacctttc cctattcagt
    15001 ctttgcgatc caaaatcagt tgggtaaggg aaaagagatt ctcttttgtt cgaagagagg
    15061 tggcaggtaa tttctttatt tccttcaaag ccttttctgt gtaagaggcc gctaacttcc
    15121 tagcttcttc cacgcctcct gtatgttcaa cgattttgcg tacggcgtcg acatcttctt
    15181 ctgtcattgc cgctttcttc tctaaataag gtagtagctt tggtttgttt ttttgcaaag
    15241 agtagatcaa cggtaaagaa tagacccctt gacgcatgtc ttctagtaca ggtttgccga
    15301 tttcctggct ctcttttgta taatctaaaa tatcatcgat aatctgaaag gccatcccga
    15361 tagaaagtcc gatatctcct gctttttttg cgaagcgctc gcttgttcca ctttcaaaag
    15421 cgccaacaga acaacttaat tggaacagtt cagctgtctt acctgaaata ttttccaaat
    15481 attcttttac agaaagttca taattatagc gatcatccat ctgccctaat tcgccattca
    15541 atactttttc catactgcga gaattcaatt ggagactttt caaggaagtg gagtaatcag
    15601 acaatagttt aaaacagcag acaaataaat agtctcctgc atagacagaa acgacattac
    15661 caaactgact acggatatta ggctgaccac gtcgcagatc cgcttcatca acgatatcat
    15721 catggatcag tgtggccgta tggagcattt cgatagaagc tgccaaagcg atggctttgt
    15781 ctcgatcttg ctctggtcca aattgtgaga acaatagctg ataggcagga cgcaacagct
    15841 ttccgccggc atggatcgtt tctttgatgg cttgttcgac aggtttgttt ttcaggcgga
    15901 cagtttcttc catccgtttt agtgtttctg tcaattcttt acttaaagca ggatagtttt
    15961 cccacattgg atgtagcttc ataaaaaaac tccttgtgtg acgattattt tttggtaaac
    16021 attgggctcc atttttgttt ggttccctgc tcataatctt tttggaatcg ttgaagtgtc
    16081 tgcacatgtc gtaaaagata attcgctgcg atcccgattg caatccctga taaaatccct
    16141 aaaactgaaa ggactggcaa gtacaacatg actgtccatg attgtgcaat gaaagaagcc
    16201 atcaaaagct gtccgacatt atgcatgaaa ccgccggccg cactaattcc gatgatactg
    16261 acacgttttg gtcctaattg cttgaccaat aacattccaa aataactaag caaagcacca
    16321 ctcatactat aaaggaatgt ggaaatcgtt ccgcctagca aagtagtcag gaatagtcgt
    16381 agccaaagta gtaagaagct gtcttgtttt ttcatggtaa aaatggcaac gatcgtgatt
    16441 agattggcta agcctagttt tgcaccaggc gcaaaagaaa aaggataagg gatcatattt
    16501 tccaataaac caatgatcac tccttgtgca acaagtagcg aaatataaat cattttttgt
    16561 aatttactca tggtgtgtga aggtctaata aatcagactg tcttcgtcac ctccatctgt
    16621 tgaacggact tcgatcacta atttatgtgg caaacagacg atggtttcgc catttttcgt
    16681 tgcccagcct cgacgtacgc agatctggtc atcgcaatct gcttctttta tccgtactcg
    16741 atcgccatct atctcgatca agttataatc tccgtgttca tctttgtaag tatatgtgtt
    16801 gctctcttct ccagccatca aagggaatgt tttgatttct gtgccatcca cacgcagtac
    16861 cgcttctttt tctgctgttg cggtgttttg ctgaagacca aaaatcatga ctggtaaaaa
    16921 agaaagaaca gtcaaaagca aaataataaa aacatcccaa ggtttgagca gacttttttc
    16981 agcaaattct ttaaggctca tttttgttct cctttttcca gattaactca cccaatttta
    17041 gcataaaaac aagaggttgt gacacctgtg tcttgctttg agtaataaga acgaagaata
    17101 gaaaacagct ttccatgttt tctaattctt tgggcttatt accgagaagc agcaggtgga
    17161 acaccgttta taacaagagg ttgtgacaga agtgggcaac ttcaagaaat aagaaggaat
    17221 ttacgaaaat tacttttcaa gtttttgtga aatttcggct tatttttcga agaagctact
    17281 tctggaacac cgtttataac aagaggttgt aacagaggtt gtgacacctg tgtcttgctc
    17341 atggaggctt atctcgggca tcttattcct ttaattttac ataaaaagga ggctgtgacg
    17401 gtttgtctca gcccctttct attgaaatct tgcattcaaa taacgttctt tatctattta
    17461 tttcttgccg tactgggaac ttggtctacc gtaccaccaa ttacctagtg tacgttgtag
    17521 ccatggaatt acccagcgat ccaacccaat cgcacgtccg gaaccgttca tcaatgcgaa
    17581 agcaacaaaa atgaaccaaa tgtttaccca gtaaaacatg cctgataagc agaacgtgat
    17641 caccaataca atcgttgtcg cactgcatag ccatgtgaat aatccagcaa tcaatgctaa
    17701 tgccaaggca atttcaaaga ataccatgaa tttttggaag aacattgcta catcattgtt
    17761 tggcatgatc acttgcatga tctttgtgaa ccagtctggt gacttctcaa tcacagccat
    17821 tggttcttca ccgtatgcat aactcaaacc aaaatgtgca gcttgagtcg ctgcttctcc
    17881 ggcacttgcc cctgctccgc tggcagctgt ggttgcatca ccagcagcag aagcaccact
    17941 ggcaacatct gttacattgt attcccatgt ccaagggaac acactgtttc cagtgaacca
    18001 tgatccttca ccaaaccaag tacttggttt gaacacttca ccattcccaa tgattttttt
    18061 ggaagcttca acgaaccaaa ctaaaccata gaaaatacga agtggcacac tccataagac
    18121 attgccgtaa cgagaagaat gtcctcgagt cacgttgcga tcatctttga tatggaaaaa
    18181 ctcatgcatt aaatattgga acatataata accagaacga atatcaaaga agtatttcaa
    18241 attcacgata tgtttcatta cgatagctaa aaatccgctt aaatggatct tatcaaatag
    18301 gttagctacc ccccatttgg caccaataga aaccataaat ccttgatagt ttcctttaaa
    18361 tttatgcttt tctccgcctt tgatcgaagc cacgatattc gctgcggctg tgtgtcctgt
    18421 ttgttcagca gcttgaacga tctgcggtgt cggtgttcct gggaattctt cataaaagac
    18481 taaatcacca atgatataga tgtttttatc ttcgtagcct tttgcctgca tatattcatt
    18541 ggcgatcaac cgctgtccgc gtgctgattc taatccaaag tcagcagcgt cagaagttcc
    18601 tttgacacca gctgtccaaa tcagtgtgtg agtcggaatg ttgcgtccgt ctttcaattt
    18661 gatatgatca gcagccactt ctacgattgg tgcattcaat aataattcca cgttgtgctt
    18721 acgcaaatag cgttcagctt ttgcagcatc atttcgatcc aacatattca agatcgtcgg
    18781 cattgcctct acgaccatca gtgtgatttc gtttggatct aatttataat ctttggctag
    18841 gcgatttttc cagtcgatca gttccccaat catttcaata ccggtaaatc cagaaccaca
    18901 gacaacgaaa gtcagcatgg ctttacgcag ttcatcatct ggttccaatg cagcttttgc
    18961 tactgtcttc tcaatatgtt cccgaatcct tacagaatct tcaaatgacc aaagggtgaa
    19021 tccgtgtgct ttgacaccag gtgtaccaaa gtcattcggc tctccgccca ttccaaggat
    19081 cagctgatca aaaggatagg aacctaattt ggtcttgacg atttttcgtt ctttatcgat
    19141 accgattaca gtgtctgtga caagttgtac attcttttga cgagcaaaca aatgctgcag
    19201 atcgtattgg attgcttctg gttctacacg tccgccagct acttcatgca actcggtcat
    19261 catagtatgg tacgaatgac gatcgatcag agtaattgct acatcttctt ttttcagttt
    19321 ctttgccaag tattttgtag ctgaaacacc ggcatatcct gctcctacaa cgacaatatt
    19381 ttgctttgcc attgatcatc cgctccttat agactatc
//
LOCUS       DAHEYX010000035        19235 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHEYX010000035 DAHEYX010000000
VERSION     DAHEYX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.035"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..915)
                     /locus_tag="MB219_000840"
     CDS             complement(<1..915)
                     /locus_tag="MB219_000840"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCC8426574.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSA"
     gene            complement(1212..2156)
                     /locus_tag="MB219_000841"
     CDS             complement(1212..2156)
                     /locus_tag="MB219_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426575.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKFRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2376..3569
                     /locus_tag="MB219_000842"
     CDS             2376..3569
                     /locus_tag="MB219_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8426576.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYVTRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAGQLVQLGWQYTFLIYA
                     ISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVYNAVSI
                     KLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLLMGIGF
                     VLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKHAESFNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            3591..5606
                     /locus_tag="MB219_000843"
     CDS             3591..5606
                     /locus_tag="MB219_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCC8426577.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TETGGGILFDFGRAVNGCVELISLDDHIESITLCYRESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5689..7062)
                     /locus_tag="MB219_000844"
     CDS             complement(5689..7062)
                     /locus_tag="MB219_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCC8426578.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7088..7372)
                     /locus_tag="MB219_000845"
     CDS             complement(7088..7372)
                     /locus_tag="MB219_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8426579.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7374..9389)
                     /locus_tag="MB219_000846"
     CDS             complement(7374..9389)
                     /locus_tag="MB219_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCC8426580.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     THIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFDSFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDDPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9568..9873)
                     /locus_tag="MB219_000847"
     CDS             complement(9568..9873)
                     /locus_tag="MB219_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCC8426581.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHY"
     gene            complement(9962..10993)
                     /locus_tag="MB219_000848"
     CDS             complement(9962..10993)
                     /locus_tag="MB219_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8426582.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNILVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIKSGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTTNSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11200..11388
                     /locus_tag="MB219_000849"
     CDS             11200..11388
                     /locus_tag="MB219_000849"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCC8426583.1"
                     /translation="MLIEEKLTKQELFTTTEKRIADYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKNGIQRI"
     gene            11369..12076
                     /locus_tag="MB219_000850"
     CDS             11369..12076
                     /locus_tag="MB219_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCC8426584.1"
                     /translation="MGFSGFKEFRFELGWEVHQLIAQFNQTIDPNFPFSSEDSTQQIA
                     KKMADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKL
                     NKFLIMAEEYSDSSWNAANLISEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGN
                     QHSEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLK
                     NKQELMQKGLLKEYRKT"
     gene            complement(12228..12821)
                     /locus_tag="MB219_000851"
     CDS             complement(12228..12821)
                     /locus_tag="MB219_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCC8426585.1"
                     /translation="MHNKIHNKERLLIWAFFLKQIQLTIILDHNFFDLLFFDWKWQRA
                     SSVLLEGTSYPTGGENTHAVISSHRGLPQAKLFTDLPKLTLGDSFYIEINGRTLAYQV
                     DQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMVNSHRLLVRGHRVPYHAKEVQKEVQK
                     VSENKRFFLYGLLAACILIFLLLIYLFRQSFKIRNRS"
     gene            12825..13553
                     /locus_tag="MB219_000852"
     CDS             12825..13553
                     /locus_tag="MB219_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCC8426586.1"
                     /translation="MRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGKLKYSSYI
                     KVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSSDCKEKNEEYVVQLLTQIYFF
                     SRIFPYDTTHLTICATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFW
                     MLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSK
                     YNLLEYDLNVSFANEEELIRLFEL"
     gene            complement(13626..14273)
                     /locus_tag="MB219_000853"
     CDS             complement(13626..14273)
                     /locus_tag="MB219_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCC8426587.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKIGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNKKWLELLRI
                     NHFSIQSSEEAVSFLYFSMSLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(14281..14967)
                     /locus_tag="MB219_000854"
     CDS             complement(14281..14967)
                     /locus_tag="MB219_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426588.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(14973..16046)
                     /locus_tag="MB219_000855"
     CDS             complement(14973..16046)
                     /locus_tag="MB219_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426589.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEEGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHELKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            16269..16943
                     /locus_tag="MB219_000856"
     CDS             16269..16943
                     /locus_tag="MB219_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426590.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIETLRNVG
                     YILQRV"
     gene            16948..18288
                     /locus_tag="MB219_000857"
     CDS             16948..18288
                     /locus_tag="MB219_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCC8426591.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            18377..19235
                     /locus_tag="MB219_000858"
                     /pseudo
     CDS             18377..19235
                     /locus_tag="MB219_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         6027 a         3596 c         3496 g         6116 t
ORIGIN      
        1 agcacttgtt gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac
       61 ttcacgaaca ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc
      121 gtattcacca gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc
      181 ttttacgata cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat
      241 gatctcataa gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat
      301 acgatggtct ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca
      361 tgcagcaact tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc
      421 aacttttaat ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt
      481 accgataaca cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc
      541 tactgggttt gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac
      601 gatttggcgc atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt
      661 ttggttgata ccagctgtga taacaacgat atcagcatct ttacactctt catagccacc
      721 tgaccataca gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc
      781 cccttctgct ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt
      841 gatcattgca taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg
      901 acttgttttt ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa
      961 gtatagtctt gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt
     1021 ttgatggaaa cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata
     1081 tcccctgata atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa aattcgctgc
     1141 aacgatataa taagacagca accgcaccta aaacaatttt ccttcatatg ctttagtgaa
     1201 agattttctt cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg
     1261 aataatttga ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac
     1321 gaaaaactcg tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt
     1381 tcttgaaagg aagcatcaaa atacattttc aatttattac taagatagac cggattgtag
     1441 ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct
     1501 aatactctaa gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct
     1561 aataaatgag tcaattcgat cagtaatagt gatgtataga gatttaacga acggaactta
     1621 tgactgtatg tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt
     1681 gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattg
     1741 atcaaaaaag aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat
     1801 aaggtttctt cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt
     1861 ctttgagtag cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct
     1921 ccattgataa tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt
     1981 gtatgctcgg gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga
     2041 aatagatctc ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca
     2101 ttgatattca ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa
     2161 gtgtctaatt cagctagttc caattgactc cctccttttt gtaagtggtt tctttcgctt
     2221 attagtttac gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc
     2281 tatggttggg acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta
     2341 tatttttgat aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga
     2401 aattttctct gctgtcagtc tctcttattc tgacttctgc tggtgcagtc acgtctgcaa
     2461 ttcccagtat gtcagctact tatgtcacgc gttctttatc tgctgttgaa ttattaacga
     2521 ctgctccctc ctttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa
     2581 tcggacaaaa acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc
     2641 ccctcttctc acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct
     2701 ttggattgat caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac
     2761 gagcctctat gattggtttt caaagtgcat ttcaaggatt tggtgcagga cagctagtac
     2821 agttaggttg gcaatatacc tttttaatct acgccatcag cttacctatt cttgtcttgt
     2881 ttgttttgtt tgttcctaca cctgaaacaa ctgaacttca atcacatggc acagccccaa
     2941 caagacaagt tatcaaaacg aatcctaaaa tgattagtta tggtattttt ctcttgctgg
     3001 tcatcgtggt atataatgct gtatcgatta aattagccac agtcctgacg acttattctc
     3061 taggaaacga aactaatgca gcgaatatct taacaatcat gcaattggca agtatggctg
     3121 ccggagtttg ttttggctgg cttcagacaa aagcacatcg ctatatgctt acactttctt
     3181 tactactgat gggaatcggc tttgtattga ttgccactgt gataaacctg tatgtgattg
     3241 ggtttggtgc gatcctaaca ggaatttctt tttcgttatt cattccttac ttattcaacc
     3301 aagtaagtat ccaagcgcct aagcacgcgg agagtttcaa tacttcaatc ttattggttg
     3361 gcgctaattt aggtactttc attgcaccat atggtttgct ctttctctct tttgcttctc
     3421 ttgggagtaa aacacttgct gttttcgtca atggcagtat cttgctgttg attagcgcgc
     3481 ttattagcct tctagtagta cacaggacga atagaaagac agtgataaga gtagatgaag
     3541 aaataaaaga acaattgaca acaaaataaa tttgatggag gtcgtgtaaa atgacaaatg
     3601 aaaaacataa agatagatgg ctttggtatc ctggagactt tgagatccgc catggactgc
     3661 tacaaaattt ccaacgggaa gaacgaggtt ttgactggcc agcgtactgg tacatggatg
     3721 attgccatcg gaacgtcaag tttaagagat attactttct tgatcaacct tccatgttta
     3781 aagtgacgat ccaaggagtt gggtatgtag aaattaatgg acaaaaacat ccatgtggaa
     3841 aatggctgac ttgtccagct ggtaaagcaa aaattcgtat ctttgtagga catacatcag
     3901 ggctaccagc gatgtttatc gagggagacg aagtaaaatc agatattggc tggacagcaa
     3961 gtaattttat tgaagaatat ccagctggct ggagtccgct ttatgtcgat atcgcaaaag
     4021 acccaaatca aatctattat caaaaagaat gcgtccatcc agtcactgaa acggagactg
     4081 gcggaggaat cttattcgat tttgggcgag cagtgaatgg ctgtgtagaa ctaatatctc
     4141 tggatgacca catcgaatct atcactctat gttatagaga gtccgatgta gaagctatgg
     4201 atatcgatta ttgctactat aagcaaatag gaatgacaag aaaaatgaaa gctcgtaaac
     4261 gcgcttttcg ctatttgttt ataccagagg tttctactgg aatggttcaa cttcaagttt
     4321 ttcatgaatt tttatctctt tcgaaacaag ctgctttttc aagcgattct tctttactga
     4381 atcagatctg gtcagtatca gaagaaacct tcaagttatg tagtgatttg ttttttatcg
     4441 acggtatcaa acgagatcgc tggatttggt ctggagatgc gtatcaatca ttcatgatca
     4501 atcaatatct tttttttgac gaggaaatca ataaacgaac gatattagca ttaagaggac
     4561 aaaatgaaat caagcagcat atgaatacta tcgttgacta ttcgatcttg tggttgattg
     4621 gtatcgaaaa ccactatatg atgacgaaag actatgaatt tttacgcatc gtctacccta
     4681 agatgcaatc attaatgaga tacttgatgg agcaaacgaa tgaattaggg tttatttatg
     4741 ggagagcaaa agattggata tttattgatt ggtctgaaat ggataaagaa ggaacgcttg
     4801 ctgcagaaca agtcttatta ctgaaagcct atcattccat gatcacttgc ggagatatct
     4861 tagctgaaac atatggagag aacgaaaatt atcatactca tctctatcaa gaaaaatatc
     4921 aactattgaa acaaaaccta tttgactact tttgggatga agaacttggc gcttttatcg
     4981 attgctatga atctgggaga agaaatgtga cgcgtcatgc gaatattttc gctgttttat
     5041 tcgattttgc taacaaagac caacagcaat cgatcttaaa gaatgtctta ttgaatgaca
     5101 ctatcacaca gattactact ccatatttca aattctatga acaagatgct ttatgcaaac
     5161 tcggacagac gaatatcgtc taccaaacga ttcttgatta ttggggaggg atgttatctc
     5221 atgatgcggt aactttctgg gaagaatacg accctactca aacaggaaat gaaaagtaca
     5281 gtatgtatgg agatccttat ggaaaaagct tatgccatgc atggggagca agcccaatct
     5341 atcttttagg acgctatttt atcggtttgt atccaacaaa gccaggttat gaagaattta
     5401 tgatacaacc taatctagat tcatttgaaa cactgaattg tacgctacct atcaaagatg
     5461 gaaagatttc gcttgaaaaa acaaaagata agatcgtgat tacttctgat agaacaggcg
     5521 gaacagtgaa aatgaatggt aaaagttatc ctcttctagc aaaacagcca cttgtcttaa
     5581 caagctattc aaaaataaat gcataaaaca aaaaactgct tgcgacatat cagaagactt
     5641 taaatcttct tctgatattg tccaagcaat ttttattttg aagtttgctt aattctgttc
     5701 tttagaaaca atgtttgttc tttttccggc aaaaagaaag ctagataata cgacaagtaa
     5761 aaagaaagcg atgattccaa ctgtcaaggc tatttgaccg tattctccta gtttcccaag
     5821 tacaagtcct ggtactagat aatcagtatc ggcaaatgta gaagaagcga ttttaagatc
     5881 tcctaatact ggaaggataa ataatggcag ccaactaatc aataagctat tgaaagcaga
     5941 acctaatacc gcccctcttc taccgccagt cgagttgccg aagacaccag aagcagctcc
     6001 gcagaagaaa tgtcctacta ccccaggaat gataactgta gtttgcaagc ctatcatagc
     6061 aaacatggat agtgttccta caaagaagct aacaaaaaag ccaattaata cagcattagg
     6121 tgcatatgga aaaacgatag gaacatctaa tgctggttta gaatcgggta cgagtttttt
     6181 agcaatccct tggaatgctg gaacgatttc tgccaacacc attcgaaccc cttgaaggac
     6241 gataacaaac ccagctgtga atgttccagc ttggatcagt gcaaagatta cagcgttgtc
     6301 tccattgctt agttctcttt caacaaatcc aggaccagct aacaaagcaa gtgcgacata
     6361 tacgatcgac atcaagagca tgatcgatac agtagaatct cgcaaaaagc tgaaactttt
     6421 aggaatcttt atgtcctctg tagaacggtc tttgtttcca aaaaactttc cgatccatcc
     6481 actagaagca taaccgatat ttccggtatg ccctaacgca acttgattgt ttccagtgat
     6541 gttcttcata aacggttgac aaagagcagg cgtaatagtt aatagtgttc cttcgaagac
     6601 cccgcctaat aagatcatcc acaaatttgc agtaaacccc gcgcttacta aaatgactgc
     6661 tgttagacaa gaaacataaa gcattgcttg acctgtaaga aaaatgaatt tatatcttgt
     6721 gaaccgagca agtaaaatat tcacgatcat ccctactagc atgatcaaag cagtaggtgt
     6781 accgtattgt tgcaaagcaa tcgccactac tgcttcattg ctaggaacta ctccttgagt
     6841 atgcagagct gtttggaaca tcgaagcaaa aggggttagt gaattactga ttagaccagc
     6901 accgccgatc agtactagga atccgacaaa tgttttgata gttccttgga tcacttctga
     6961 aaatttcttc ttctgaagaa cgagaccaag cagtgcaatc aaagaaacga ggatggaggg
     7021 ctgactgacg attgacacaa acaaattcaa caatttatcc attgatagtt cctcccttga
     7081 attattttta ttcttttgac aagatcttcg ttactttttc ttttacttct tcgctgtcaa
     7141 tgatcgattg caaaggtacg agcttttcat caggtataga agttagcgta tctttcaaag
     7201 aatgctcaat gaagaaataa tcagccatat cgctagatac acttccaaca tccgtatgac
     7261 ttacttcaaa ctcgtccaca tctgctccaa gatctgcaag tgcttgtttg atgttcatct
     7321 ctaccataaa acttgttcct aatccacttt gacatactgc aaaaattttc atatcattcg
     7381 tctccttttc ttatgatcgc taatatttct tcttttgttg atgcgtgtaa taaatcttga
     7441 agattttgtt tattagaaag tagctttgtt aaatttgcca acgcccgaag atgcgcctca
     7501 ttatcgatag cagctaaaca gataaacaaa ctaatgggat gggcaggatc atcaagcaga
     7561 tccacaggtt gttgaagttt cagcaaagac atccctactt catttacgcc atcttctgga
     7621 cgagcatgag gtaatgcaat gtggtcgcca atatggataa acgcaccata ctgctccacg
     7681 cgctcgatca tagctttgat ataactttct tctattttat tttctgataa tagcggctgt
     7741 gcggcaaatg tgattgcttc ttgccagttt tttggttgat ccgataattg gatcatttct
     7801 tctgttaata attcagatag cataggacgt tgatcctcct tcttttctaa aagtttgttc
     7861 attttacgat ttaatacttg atagagcttt tcttcgtcta cgccttcttt taattcgata
     7921 taaggcagta gcgccgacaa caattcttgg gcagaaggca ttgaaaatcc tggtatcagc
     7981 caatctcttt gtacttgatt gatcagctga tttttttcca agtcagtcat gattggatgc
     8041 agaacataga gagacttttt cttatcagta agtgtgagag gtacagtaga aaaaataata
     8101 tcgtaagaag tttcgtcaac ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag
     8161 atcatcggga ataattgctg aagttcactt tttaagatca atgaagagct aatgccactt
     8221 ggacagacga tcacggctct tatttttcta tttctttctt ctgcgtccgc ttttctgatc
     8281 tctccgccaa atagtatagt gaaaaaacca atctcttctt cagggatgct cttccctatt
     8341 cgtctttcta acggaagtaa tgcttttctt gtcatttcgt agatggaagc atattgatgc
     8401 ttaatttgat cgatcatcac attaggtaaa taaaaagaat aggaaatacg aaaataggca
     8461 gggaccaagt gataaaagac attcatcaat aattcacgaa acgaatcaaa ttctacggcc
     8521 atcaggaatt ccatgcgatg gatgacttct gaagaacatt ccagcaaaaa atcaagtgca
     8581 ggctcctgga catctccttc aacgatcgat gctaagcaaa gagagaaaaa tataatctcc
     8641 gaattttgat gttcggaata gccaaggcca gtcaatactt gtcgactaat gtcagtgact
     8701 gcaagagaag ccaacagttc agcatctttt accagttcca tggatgtttt tccaattcga
     8761 atggttgtca aactcaaata ataagctaat tcatccattc gatttggtgc gaatcgaaga
     8821 tctgatagtg aaatcgtttt atctagcaaa tctctaactt tggcgtaata atcaatggaa
     8881 agggttgcgg ttaaatacgt gagcccaaat cttccagcaa gtgactgcat caaacgagca
     8941 gtgcgatttt ttgcttctct tcttaatatt gcttctgacc ctagcagcct aaaaccagtt
     9001 ttcctagtat acgacagctg acaatcttcc acttctaatt catttcttat ctttttgata
     9061 tctgacagga ctgtcccttt agaaacctga agaaaatctt gaaaatgaaa aacagaaaca
     9121 gacgttgcag tcgaataaac catcaaataa agaagatctc ttcgctcttg ttcggtatac
     9181 acagcttcaa atcgagtaat atccaaaaat ttttctgccc agtcgattgc tcggaatttc
     9241 tttatacgga tatgggtatc atccacatat tcggcaaaag gagttaattg tttgatcgcc
     9301 tcgcttgtat ttacttcgat gatctctgct gctttttcta gactgatttc tcttggtgtt
     9361 ttcaggagat aactaatttt gtagttcaca gcgctgttca cctcctactt atattctagt
     9421 atggtagcgc ttttaaatcg aggggtgttt ggtacaactt acagttggat tttttttacg
     9481 aatgtaccga acgacttcta tcgaatggat acagaaaaaa agcaggtgac caaatgtctc
     9541 ctgcttttat gaattgattt cgtccgatca ataatgatca tgtttatttt ttgaggtcag
     9601 ttcttcaaat ttttctttga catcttcaac cgctttttcg atatcttttt caaagtgatt
     9661 gggctcatta ggctttgttt cctcttctac agaatttgaa ccagataaag ctgtttcttt
     9721 attttctgtt tcaccagagt ttgtgccagc taatgattct tctttgtctt ctgtttcacc
     9781 agaattagtt cctgctaaag ccacttcttc ttctgtttta ggggccattg aacctcttaa
     9841 tcgtttgtgt ttttcaccgt cataatttgt catgttggtt cctcctagta gttctatact
     9901 ttttcatcgt acaataagac agtttatgcg tcaaaaaaca aacccgattc ttacaaatat
     9961 gtcatatttt tccttctgat tcttgaatgc ttttgattcc ttcttccaaa attttcattt
    10021 gtaagattgt ttcttcatct ttgatgactt tttctgcgcc attaactaat gtctcgtaaa
    10081 tcccttcgta gacacggctg taatcaccaa cctcggaaat tactttttct tcatgataac
    10141 tcccttgttc atctatatag gtcaatacac cataatgttc gggcaaatcg ataccaaagt
    10201 cttcatgatc aggcatgtaa aacattttca gatgttcttc ttgtcgatct tttgtttgtt
    10261 tcacgaacat cccttttttt ccgtaaacga cgaaacttgg acgttctttg atgcggaaat
    10321 aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg aagtaatcat
    10381 tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct ggttcgccga
    10441 aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag ctgttcgtag
    10501 tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa tgcatttcta
    10561 cttctaaaat atcccctaat tttccgcttt tgattacttt ttggactgtc aagaagtcag
    10621 aatcgaaccg tctgttttgg taacattgga taaatagccc tttttctttt gctaatgcaa
    10681 ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt atattcttac
    10741 catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta gtgatgacaa
    10801 ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat tcgatgtctt
    10861 cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg attttgttcg
    10921 taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg ccaagataag
    10981 cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc ctagaaaagc
    11041 gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac ttgttattat
    11101 tcggtttgat gaaattaatg ttcaaagtaa ggataaaaat caaaaagata gtagcggaac
    11161 caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga agaaaaacta
    11221 acaaaacaag agttgtttac cacgacagag aaacggattg ccgattacat ccgtaggaat
    11281 atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta tacttcccat
    11341 tcagctatca ttcgtttatg caaaaaaaat gggattcagc ggatttaaag aattccgttt
    11401 cgagttaggg tgggaagttc atcaattgat tgctcagttc aatcaaacga ttgatcctaa
    11461 ttttcctttt tcaagtgaag atagcactca gcaaattgca aagaaaatgg cggatttatc
    11521 gatccagtca ataaaaaaag cacaattgca gattgaacat gaagatttag atcaaatcgg
    11581 taaagtattg aacaaggcac agcgaatctt tctttttgca aaaggtgatt ctcaaatcac
    11641 ggcaaggaaa ttccagaata aaatggtcaa gttgaataag tttttgatca tggccgaaga
    11701 atacagtgat tcttcttgga atgcagccaa tttgattagc gaggattgcg ccatttttat
    11761 tagctacagc ggtcgcatcc atcattatga acgaataatg acctatttga agcatgtaga
    11821 ggcccctacc ttgctgatta ccggaaatca gcattcagaa atggccaaac aggctgatat
    11881 gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa gtagcaactt tctcctctca
    11941 aattgccttc gattacgtat taaatacgtt attctcagtg atatattctc aaaattttga
    12001 agagaatatc cgcaacttga aaaacaaaca agaattgatg caaaaaggcc ttcttaaaga
    12061 atatcggaaa acttaagaaa agatcctcat ttagtaatga ggaagatagt gtttgatagg
    12121 ctctctttga tatcatcata aaaacaggct gaagttttct ttttcaaact tcagcctgtt
    12181 tgtttacgga acgattcgaa tagatatatt atagaaatga ggaatagcta acttctgttt
    12241 cttattttga acgattgacg aaaaagatat ataagaagga ggaaaatcag tatacatgca
    12301 gctaacaatc catacaggaa aaatcgtttg ttctcggata ctttctgtac ttctttttga
    12361 acttcttttg catgataagg aacccggtgc cctctgacaa gtaagcgatg actgttgacc
    12421 atataaggtg tacaagttag gagtgtcacg aaatcctgcc ctttttcgat tctcagatct
    12481 tttgtatcag tcggttctac cgtcttgatc tgatcgactt gataagcgag tgtccgtcca
    12541 ttgatttcga tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt gaacaacttt
    12601 gcttgaggca gtcctcgatg gctggaaata acggcatgtg tattttctcc ccccgtagga
    12661 taagacgttc cttccaataa tacggaagac gcacgctgcc atttccaatc gaaaaaaaga
    12721 agatcaaaga aattatggtc aagaataatt gtcagttgaa tttgtttcag gaagaaagcc
    12781 catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga gaatcccaag
    12841 gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat atcgataaaa
    12901 aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc tatataaaag
    12961 tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa aaaaatgaaa
    13021 tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatcctct gattgcaaag
    13081 aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc tcccgtattt
    13141 tcccatacga tacgactcat ttgacgatct gtgcaactta ctttgcacag cagatgcaac
    13201 accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat tccactctac
    13261 tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt atcggtaatc
    13321 ctgaaattca ccattatact tctgataaaa aaatactgat catcagtgac ttaagtcttc
    13381 gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat aaaattcact
    13441 ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc gagtatgatc
    13501 tgaatgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca
    13561 aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt
    13621 tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac
    13681 tcttcttttg tccaattatt tgcaatgaga tccgtcaatg tgttcattac gagggacatg
    13741 gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt
    13801 aataattcca gccatttttt gttcattgga gaatcagccg atagttctct acgtttggtt
    13861 gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga
    13921 tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt
    13981 tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac
    14041 aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccctatc
    14101 ttgtcaacga tctctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt
    14161 aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc
    14221 atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat
    14281 tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc
    14341 actccattga atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg
    14401 agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt
    14461 tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct
    14521 ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa
    14581 agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac
    14641 tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa
    14701 tccaatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagagagtg
    14761 accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa
    14821 agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata
    14881 aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac
    14941 ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct
    15001 actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcaacaat gatcaaaatc
    15061 ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc
    15121 agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag
    15181 gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact
    15241 ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac cacagcagca
    15301 accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca
    15361 ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct
    15421 ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa
    15481 gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg
    15541 ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa
    15601 ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg
    15661 atgatttcat tctttgtttt aagttcatgc cctttcgtga gttttggtaa taaaaatgct
    15721 tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg
    15781 tacaatccga ttggtgcttt ctctccttct tcaatgtatt tcagatctcc tcttgttaat
    15841 tgtgaagctg caaatacttg atttgcatcc tccgataaaa caatctcttg tgcgtcccaa
    15901 tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat
    15961 acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag
    16021 cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac
    16081 aaaatgtcac tcgttgaata gtgtaactcc aaagtgacat tttgtcactt atgttgtttt
    16141 ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa
    16201 aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag
    16261 gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa
    16321 aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt
    16381 tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga
    16441 atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt
    16501 tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt
    16561 atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac
    16621 gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa
    16681 aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat
    16741 tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc
    16801 ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact
    16861 tacgaaaaca tctgcgaagt actggattgg ataaagaaat cgaaacctta cgtaatgtcg
    16921 gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt
    16981 cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt
    17041 actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata
    17101 aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt
    17161 ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca
    17221 tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat
    17281 aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat
    17341 ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc
    17401 cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta
    17461 gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt
    17521 atgattgttt tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg
    17581 cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa
    17641 ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat
    17701 catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc
    17761 actggattga cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt
    17821 tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag
    17881 atggccacca tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca
    17941 attgacgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat
    18001 acgaatccag aagataagat cattttggac tcatctgtta accataaaga atggctgatt
    18061 cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga
    18121 ttttatcggg aagatcaatc tagggcgaaa gagactggcg gatacgggct tggcttggca
    18181 attgcaaaac aggtaatcga acagcatcat ggacaaattt ctgtatccaa ttatcttcca
    18241 caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta
    18301 aaaaagggct gtgacaaatg taccgacccc caaaagttag accaaaaaat ctaacttttg
    18361 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
    18421 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
    18481 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
    18541 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
    18601 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
    18661 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
    18721 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
    18781 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
    18841 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
    18901 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
    18961 gagcttctag aaactttaaa gttccctaag gcaaccttta tgtactggca aaaacgattg
    19021 gaccgtaaaa atccagatca agggattgaa gcggaaatgc ttaaagttcg ggagaagcac
    19081 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
    19141 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
    19201 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DAHEYX010000036        18901 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHEYX010000036 DAHEYX010000000
VERSION     DAHEYX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.036"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..202)
                     /locus_tag="MB219_000859"
     CDS             complement(<1..202)
                     /locus_tag="MB219_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCC8426592.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEK"
     gene            complement(199..717)
                     /gene="msrA"
                     /locus_tag="MB219_000860"
     CDS             complement(199..717)
                     /gene="msrA"
                     /locus_tag="MB219_000860"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCC8426593.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(737..1450)
                     /locus_tag="MB219_000861"
     CDS             complement(737..1450)
                     /locus_tag="MB219_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCC8426594.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1453..2349)
                     /locus_tag="MB219_000862"
     CDS             complement(1453..2349)
                     /locus_tag="MB219_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCC8426595.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2507..3349)
                     /locus_tag="MB219_000863"
     CDS             complement(2507..3349)
                     /locus_tag="MB219_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCC8426596.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3481..4134
                     /locus_tag="MB219_000864"
     CDS             3481..4134
                     /locus_tag="MB219_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCC8426597.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4197..4724)
                     /locus_tag="MB219_000865"
     CDS             complement(4197..4724)
                     /locus_tag="MB219_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCC8426598.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4744..5691)
                     /locus_tag="MB219_000866"
     CDS             complement(4744..5691)
                     /locus_tag="MB219_000866"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCC8426599.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5703..7589)
                     /locus_tag="MB219_000867"
     CDS             complement(5703..7589)
                     /locus_tag="MB219_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCC8426600.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(7749..9827)
                     /locus_tag="MB219_000868"
     CDS             complement(7749..9827)
                     /locus_tag="MB219_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCC8426601.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9840..10115)
                     /locus_tag="MB219_000869"
     CDS             complement(9840..10115)
                     /locus_tag="MB219_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426602.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10477..10932)
                     /locus_tag="MB219_000870"
     CDS             complement(10477..10932)
                     /locus_tag="MB219_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426603.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11086..12294)
                     /locus_tag="MB219_000871"
     CDS             complement(11086..12294)
                     /locus_tag="MB219_000871"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCC8426604.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12406..12738)
                     /locus_tag="MB219_000872"
     CDS             complement(12406..12738)
                     /locus_tag="MB219_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCC8426605.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12886..13761
                     /locus_tag="MB219_000873"
     CDS             12886..13761
                     /locus_tag="MB219_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCC8426606.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13843..14424)
                     /locus_tag="MB219_000874"
     CDS             complement(13843..14424)
                     /locus_tag="MB219_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCC8426607.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14430..15689)
                     /locus_tag="MB219_000875"
     CDS             complement(14430..15689)
                     /locus_tag="MB219_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCC8426608.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15691..16737)
                     /locus_tag="MB219_000876"
     CDS             complement(15691..16737)
                     /locus_tag="MB219_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426609.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(16972..17247)
                     /locus_tag="MB219_000877"
     CDS             complement(16972..17247)
                     /locus_tag="MB219_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCC8426610.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17490..18800)
                     /gene="der"
                     /locus_tag="MB219_000878"
     CDS             complement(17490..18800)
                     /gene="der"
                     /locus_tag="MB219_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCC8426611.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
BASE COUNT         5244 a         3940 c         3218 g         6499 t
ORIGIN      
        1 atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca ttcagttcca
       61 gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg tctttaccgg
      121 cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat
      181 aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa aggcagcccg
      241 gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt gatggtcttc
      301 ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct gttcgaaacg
      361 tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt cttctgtcaa
      421 ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga aagcgtcagt
      481 tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca atgttggatc
      541 atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt gttcataagt
      601 cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac caggaagcgt
      661 gtcgaaaggc tggatcatac accagaagca gcctcctgca aatattgctc gttccattat
      721 gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc ttgctttcag
      781 tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag gtatcttttg
      841 gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct gctttaatat
      901 acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga tcatctgtat
      961 tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc acatctttga
     1021 ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca tttccgtttt
     1081 ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt acttcgaaag
     1141 taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca ctatcttttt
     1201 gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt gaattgatcg
     1261 ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt tctggttccc
     1321 gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc ccaagaagga
     1381 taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat acgtcagatc
     1441 ttgttttcat gtttattgat catcctcctt ctctaaccat tggttttgtg tctcgatcag
     1501 ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat ggaaatgatc
     1561 ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc tagttgtttg
     1621 atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa gcaaatcatt
     1681 gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc ctttgttcca
     1741 attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa atggattgta
     1801 gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt ccaatagttc
     1861 tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa
     1921 taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg tgataaaatc
     1981 agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta agatctggtc
     2041 acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat tataacggtc
     2101 ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat ctcctactcc
     2161 ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat ttggatcttc
     2221 tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag cagctaaacc
     2281 tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt
     2341 taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa aattttatca
     2401 aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata aagaaaaaga
     2461 gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa tctacataat
     2521 acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc gtatgcagta
     2581 cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct tgcgccaatt
     2641 cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct ttcagttcat
     2701 ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta gttacaggga
     2761 ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta gacagcaaac
     2821 cagttgttcg gctgattctt ccacctttta ccaagttatc tagtgtggat attccgatgt
     2881 ataattttgt attgtcttgt acttttttga cttcttcaag gattttctct actgttttac
     2941 cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg tcagtggtat
     3001 cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt gctgcttcta
     3061 ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg tcttctccta
     3121 gctcatcata catcgagatg aattctccaa ttggtggttg gcttgttttg ggcagatttt
     3181 ttgctttcgc catcatttcc ataaatcttt cgccgtctag ttggtcatca tcgggatata
     3241 ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca tcgcgtacat
     3301 ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt attttctctc
     3361 tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca atttctattt
     3421 ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg agccgttttc
     3481 atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc
     3541 acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt
     3601 gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt
     3661 cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg
     3721 ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt
     3781 tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt
     3841 gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa
     3901 gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt
     3961 tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg
     4021 ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg
     4081 tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa
     4141 aaataaagct atggatggga cagctctaaa taacagctag tctatccata gcttttttat
     4201 atcgtcaatt cgcctgtccc gctttttaca ggattctcat tacgctgata cgtttcaaat
     4261 tgatgcggat aacgattttt ttcatccatt tctccttgac tagtattgat cagtgaccaa
     4321 tcagaccagt caacaggtgg aaaataagca tctccatcga atacatgatg gatgactgta
     4381 cggtataata tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc tccagcgatg
     4441 aaaaagatac cgtctgtttc tttttccaga gcaagcacgt cttccacact atgcatgaca
     4501 tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc
     4561 aacggacggc tacccatccc ttcgaatgtt tttcgtccca tcactagtgt atttgcttct
     4621 gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc aaggaagacg gtcttctttt
     4681 ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta acattttctc atcctttctg
     4741 attctaaaca gcgataggag ctttgatcgc cggatgtggg tcatatcctt ctaattggat
     4801 atcttccatc tcaaaatcaa atactgactg cttttcctga ttcaaaacta aagtcggaaa
     4861 tgcgcgtatt tctcggctca attgttcttt catttgttcg atatgattga tataaagatg
     4921 agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat gtgcaattag
     4981 gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg cctagaaaca catccccact
     5041 gcgttgatac aattggcagc ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg
     5101 acaaggaggc aaagccatgc taggaacatc ttccggattc catgcagaaa cgatcaatct
     5161 tctagaatca ggagtctttt tgattgcttc gatcacttgt ttgatttgat cgataaagcc
     5221 accgtctttt gtttcccagt gacgccattg atagccgtag atgtgtccaa gatcgccgta
     5281 tgtttcagca aaggaatcat cggttaggat cttctcgcaa aaaagcttgc tctgcttttt
     5341 atattcttca ttgaattcag gatctgtcaa acatctatgg ccaaaatcag tcatatctgg
     5401 tccattatag tctggacttt tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt
     5461 tttattttgt aatagatagc ggatattcgt atctcctttt aaaaaccaaa gcaattcgct
     5521 tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc
     5581 aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg cctgttctgt cttctttacg
     5641 atgcccttct tccaacaatt ttcttcctaa gtcaagatac gcttgttcca tcctttttcc
     5701 tcctaatctt ctacccattc actcaagtat tcccaccgtg ccattttttc ttcaagttgt
     5761 gcttctgttt cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt atctccttga
     5821 tgattcatct cttcttggag caatgagatt ttttcttcaa gttctgcaat ctcatcttca
     5881 atcgtttccc attctttttg ttccatgtaa gataatttct tcttttcagc aggttccttg
     5941 ctctgtattt ttggtttcgc tttttcagca ggctgctggg tctctttttg cttcagtaaa
     6001 taactggaca tgtttccgta ataaagttct tgttttccgt ttccttggaa tactaataac
     6061 ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac agcacctgga
     6121 aaacttctaa aataatcttc caaaatcgtc aatgttgcta tgtccaaatc attggtcggt
     6181 tcatccaata ataaaacatt aggttgctga atcagcagtt tcaacaaaaa taaccggcgt
     6241 ttttctccgc ctgaaagttt gcctatcagt gtcccatgca taaatcttgg aaataaaaat
     6301 cgttcaagta attctgctac tccgatttga ctgccatctg ctgtttttgc ttcttcagca
     6361 gcttcttgta agtaagcaat catccgttta tctggtgcca tttcttcatt ttcttgtgta
     6421 taatacgcaa gatgaaccgt ttcaccgata gacattgtac cgctatccag gggaattctg
     6481 ccagctagaa tattaagcaa tgtcgatttt cccgcaccat ttttaccagt aatacctagt
     6541 ctttcccgag attgaatcaa taaatctaaa tgttctagca gtgtttggtc atttatacgg
     6601 taactgccat ctttgatttc cagtactttt ttccctaaac gttgtgtcgc taggttcagc
     6661 tctaaatcat cttcttggtt tacttggtac aagttctcct ttagatcttc gaaacggttg
     6721 atccgtgctt gctgcttggt gccacgtgcc tgtacgccag ccctcatcca agctaattcc
     6781 tgtttataaa gccgcttgcg cttttcttct tgttctactt ctacacgatc acgttctgct
     6841 ttttctaaga cgtaagcttc ataatttcct ttatactcgt ataattttcc aaatgaaagt
     6901 tcgaagattc ggtttgctac tctatctaga aaataacgat cgtgggtaac catcaatagt
     6961 gcgccacggt attgtttcaa atagttttcc aaccactcga tcgcttcata atctaaatgg
     7021 tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg cgccagacta
     7081 actcgttttt tctgaccacc tgataaagta ctgatttttt tatctaattc actgattcct
     7141 aatttttgca aaataatctt tgcgttggta tctgtagtcc aagcatcttc tttattcatt
     7201 tgttcttctg ctttagcata acgtttttgc acatcagggt cttctccgtt ttcccctagt
     7261 tcgatcaatg cttcctcata tgctcgaaca gtttggatca aggggctgtt tccttgaaag
     7321 actgcttgga gaacagttaa ttcatcggaa aattcagctg cttgagaaag ataaccaata
     7381 cggtaatcag ttggataaaa cactgtttgc cgatcaccgt cgctgctatc gataccggca
     7441 ataatattta agagacttgt tttacccgtc ccgttcgtcc caatcaaccc gatacggtct
     7501 ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt tttcaccata agtttttttg
     7561 aggtcagtta cttttaattc tttcaacatg ttccctcctt atcttggata ttgtatcata
     7621 aaaatagtga acttcaggaa aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa
     7681 gaacaggacc gattcgatcc tgttctgata tgttttgttt tttataaaac ttgaaaattt
     7741 ttaccttttt attttattgg actcgttttt tttgactttg ctcgaaacgc aaatcggaaa
     7801 gaaaagttat cagatggttt gttcatcgta taagattcta acaaattgat attcctgcga
     7861 tagctgttcg cagaaaacgt cgtgattttt tcagcaactt cgtattcatc aatgattttt
     7921 ctttcaagat aataacctct tagcattttt ttcgtaaaca gactatcttg ctggatcatc
     7981 cacgaagaaa tcaaattccc cgagttcatt tttattcctt caagtttgcg ttgctggata
     8041 aaaaattgaa ccatcgtttc atcataaata tcagtcaagt tatctaaact ttcaataatc
     8101 aactgagtat ttttttgata tgtctctctg atttcttgga tatcttcatc actaataaat
     8161 gtctgacgat cagcatatct tctttgccaa aacattttcg gatggagaac gattcgcatt
     8221 aacctacgga caacaatgaa ccaaaagccg ataaaagaca aaatctggct tgtgatagaa
     8281 tgttcgaatc gagaaagaaa acgggagtaa aaacgataac cattagaaga aagcttgccc
     8341 gaacgataac tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct
     8401 tgtacttctt gcttttcatc atcggttaaa tcttccaagt acagatcacg tatccgttct
     8461 tgataatttt ctatcactgc ttcagtggct aaaaattctt ttgtattacg atcaatttcc
     8521 tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc
     8581 gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga caatgatccc tatgaccaaa
     8641 gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag accgctggta aaacatcata
     8701 ttattgattg aaaatggcaa aataaaaatc gtagccaaac tgactgttcc ctttacaccg
     8761 ccaaatgtta agatcaagat ctcgttcaac tgttttttga actttttact gccatctttg
     8821 aatacataaa aaagactgat agaaatgaat ctgatcacaa aaagcatgac agagattaat
     8881 acgataatcg ctaataaaag accgtttgga tataaaccgt ctccccatac tggcgagaac
     8941 acttgcgtca attcgattcc taaaaatata aatactaatg cattcagtgt aaaagcaata
     9001 gttgtccatg tactgtggga caagctcgac aattctgcat caaacacaga gattttccta
     9061 aaaccagaag cttggagaat acctgctgcc actgcagcaa tgattccaga tacatcaaat
     9121 gcctctgcca atacataggc tagaaaagga agcaaaagtt cgatcagcag ataccctgtg
     9181 acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc
     9241 aaaaagccga tcaaagctcc cccaatacta gaaactacta gactcatccc cgcgtttact
     9301 gctgaaaaag atccggtgat cagcgcacca agagcaaact ggaaggcagt aacaccagat
     9361 gcatcattca aaagaccttc tccttccaaa atatgcatcg cctttttagg gatattcact
     9421 cgtccagaaa gagaactgac tgctaccgca tctgtcggac ctaacgctgc tccaaaagca
     9481 aagcaggcag caagtgggac acttggcaac aggaatgata acgtcgcacc taccccgaca
     9541 agggtcaaaa tgactcctcc aaatgccaag aacaaaattg tcccgaaatt tttgagaatc
     9601 gaagaaatat ctgctttttc tccttctcta aataacaatg gtgcaataat cataactaag
     9661 aacaattcag gttcaaaatc gattgattgt ccccactctg tcagacctaa taccactcca
     9721 aggaaaattt ggatcagtgg agctggaatc attggcaaaa tgcggctagc tatgttcgat
     9781 aaggtgatcg ttactgcaaa tacaataact aactcagcaa attccatcgt ccatcccctt
     9841 tattttttat tttgtgtaag catttttaaa aattgttctt ttttctttaa aattttggtt
     9901 tcgactactt caacctgtgt ttggttcccc tctaattcac actgtacacg ttttgactcc
     9961 aaacgaagca gatcatgttt cagacgttcc aactgacgat acatcaatat atttctttta
    10021 cgcaacttac aaagtatttg acggcggtca tccatcgaaa gggcgccata gcgatcatgc
    10081 aacagttttt tttgttgttc aaaactctct ctcattttcc agatctccta tacacaaact
    10141 tccatcatta tagcaaagtt ctttttggat gaaaaatatt tcattgtttc atcgtttatt
    10201 gctagttaga aatttccata aatttttcaa cgcctcattc tattccaaat agtatcagat
    10261 atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa ggatgtttac tttcccattc
    10321 ctgcctttct tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac
    10381 agtcccttat tctgaataaa cggtgtggaa gagacagcct atcagtagta ataagacaca
    10441 tctctttcac agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac
    10501 atcttctaca cgttgatctg gattgatcac gatatacaag ttacctgttt tttcgctgat
    10561 ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat atgggaagta
    10621 cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat cttcgtattt
    10681 caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat gtgtcgcaac
    10741 tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga tttcaacaaa
    10801 ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat
    10861 atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta gcgctgtcat
    10921 ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact acctcagttt
    10981 agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac gtatctgccc
    11041 actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa tcaatttttc
    11101 tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt ctacgacggc
    11161 tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc cagtttcttc
    11221 tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta aacgatcata
    11281 ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca attcttctac
    11341 ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag ctccagtttc
    11401 tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt tagaacactt
    11461 ccacgatttc aaaaaggaac gaatctcatt ttcttttaga tccagttggc tgatcaataa
    11521 tgcccatgct tggctctctt ctttcaatgg gacggatggt aattcagcaa aacgtaaaag
    11581 tgcttcgcct tcttctttca aaccgggaca gcaaatgtag cactctgttt caataaatgc
    11641 atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca catttatacg
    11701 ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga aggtttcttc
    11761 ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc gtaaggcatc
    11821 ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt ctagatcttg
    11881 cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg cattgatcgt
    11941 aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat gatctggtct
    12001 acggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt gttcatcatt
    12061 ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg gaaaaagctg
    12121 tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg gctggttcag
    12181 caagatatca cgaacacttc cacctacaaa ataagcttca tatcctgcat tctctaattt
    12241 tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt tcatttttcc
    12301 aacctttcct gcgtgtttct tgtcctgaga taaagactca gttgcatggt gactgtttga
    12361 caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact cgtccttacg
    12421 ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa aaacttcagt
    12481 taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat caccaagttc
    12541 ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat aagtgtggtt
    12601 gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag ctaatggaga
    12661 gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca tactgtataa
    12721 agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact ttaacgcaaa
    12781 tcctagataa gagcaattga gaaaatcgaa tttccatgct acatttagga aggaatttct
    12841 aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt caaacgaaaa
    12901 acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt tggtcttgtt
    12961 tacttgaaca tcgctaatca tttagcagaa gggggagtct cagggatcac cttgatctta
    13021 agagctttat ttggtattga tccagcttat acgactttat taatcaacat cccattgatt
    13081 ttactaggag gaaagatcct cggtaaacgt tccttggcct atactgttct aggtacactt
    13141 tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa tttacagcat
    13201 gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc
    13261 taccgcatgg gtggaacaac aggcggcagt gatattatcg ctcggatttt agaacaaaat
    13321 tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt acttgcttca
    13381 ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta tgttttcagt
    13441 cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact tgtcgtctcc
    13501 aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg tgtgactttc
    13561 ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc
    13621 tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa agctttcctt
    13681 tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa gccacagaca
    13741 agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa agattccaca
    13801 tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt tatgttcact
    13861 tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt catcagataa
    13921 tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag cataaagcat
    13981 ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt cagtatcttc
    14041 ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag atagatattc
    14101 tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt tccaattcaa
    14161 acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca aaaacaattt
    14221 catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg caaaattcgc
    14281 cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat aattcaagat
    14341 ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta aaaaattctt
    14401 cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc taataatgac
    14461 agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc ttgcgctctt
    14521 ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc tggttcatgt
    14581 gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc ttctaattca
    14641 ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc catctgattg
    14701 atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact taaagtaagt
    14761 aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc ttctgctcgc
    14821 tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc gacttggtaa
    14881 ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat ttcttcttct
    14941 tgaagagctt gtcctagata tagatataac gattgataat gcggattaac ttctcgaagt
    15001 tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt aaaagctagc
    15061 tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg tacgatagct
    15121 gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat cgagattcct
    15181 gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc ctctttgaaa
    15241 cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc atcaggaagc
    15301 aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat aacttggtac
    15361 aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc cactttttcc
    15421 aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc taacgggatg
    15481 ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg ttttgcttcg
    15541 atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc tgctgggtct
    15601 tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc tgataaagct
    15661 tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac tgacgatttg
    15721 ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt atccgtctat
    15781 tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa aaagaacttt
    15841 ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc caagcaagtc
    15901 cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga gattccacac
    15961 gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat ggccattttc
    16021 ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt ctgcctctaa
    16081 ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg ttaattgctt
    16141 tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa tcattccgca
    16201 acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc tgattgtttg
    16261 atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat ttggtagctg
    16321 caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata cagggatcat
    16381 ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc
    16441 ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt tgacgaaatc
    16501 aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa tataccagtc
    16561 attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat cataatagcg
    16621 ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct gactgactag
    16681 cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa gttccatacg
    16741 catccctcct cttcttattt ttattttacc attagtaagc aaattctaga aaagataaga
    16801 gcaaagctca cctcataact tgcaaataaa taaaaattaa ataaaataca tgtgttttct
    16861 atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct gctaggaaat
    16921 aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt cttatttaac
    16981 agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg gaatttcgat
    17041 ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt
    17101 accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa
    17161 tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg aagcaacttt
    17221 ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa gagatatgat
    17281 agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag aaatattgat
    17341 taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa ggtttttacc
    17401 aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc tttttctttt
    17461 gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat aattcggatg
    17521 gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg ttcataagaa
    17581 aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt gacagcgact
    17641 tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg attgattgct
    17701 acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt ctggttcata
    17761 cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca
    17821 aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg ttcgaaatca
    17881 cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat aataattcca
    17941 cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat cccttcttca
    18001 gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc acgcatcacg
    18061 ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc tgtatctatc
    18121 attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt
    18181 ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat caaagaggat
    18241 ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca
    18301 gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc tagtccatgg
    18361 cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata gatatcgttt
    18421 ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt acttctgtat
    18481 aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct gactacacaa
    18541 atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg atccataaaa
    18601 ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg acctaaccat
    18661 tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac aatcgagata
    18721 cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg gccaacgatt
    18781 gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc acttagttta
    18841 acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat ttttattcct
    18901 t
//
LOCUS       DAHEYX010000037        18538 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHEYX010000037 DAHEYX010000000
VERSION     DAHEYX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.037"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            133..780
                     /locus_tag="MB219_000879"
     CDS             133..780
                     /locus_tag="MB219_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCC8426612.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    909..1125
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1173..3818
                     /locus_tag="MB219_000880"
     CDS             1173..3818
                     /locus_tag="MB219_000880"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCC8426613.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4128..5441
                     /locus_tag="MB219_000881"
     CDS             4128..5441
                     /locus_tag="MB219_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCC8426614.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5431..6084
                     /locus_tag="MB219_000882"
     CDS             5431..6084
                     /locus_tag="MB219_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCC8426615.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6139..6831
                     /gene="radC"
                     /locus_tag="MB219_000883"
     CDS             6139..6831
                     /gene="radC"
                     /locus_tag="MB219_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCC8426616.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6981..7181
                     /locus_tag="MB219_000884"
     CDS             6981..7181
                     /locus_tag="MB219_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCC8426617.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7435..9114)
                     /locus_tag="MB219_000885"
     CDS             complement(7435..9114)
                     /locus_tag="MB219_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCC8426618.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9116..9328)
                     /locus_tag="MB219_000886"
     CDS             complement(9116..9328)
                     /locus_tag="MB219_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCC8426619.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9721..11451
                     /gene="efrA"
                     /locus_tag="MB219_000887"
     CDS             9721..11451
                     /gene="efrA"
                     /locus_tag="MB219_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCC8426620.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11448..13208
                     /gene="efrB"
                     /locus_tag="MB219_000888"
     CDS             11448..13208
                     /gene="efrB"
                     /locus_tag="MB219_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCC8426621.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13303..13500
                     /locus_tag="MB219_000889"
     CDS             13303..13500
                     /locus_tag="MB219_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426622.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13653..14138
                     /locus_tag="MB219_000890"
     CDS             13653..14138
                     /locus_tag="MB219_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCC8426623.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14242..14835)
                     /gene="clpP"
                     /locus_tag="MB219_000891"
     CDS             complement(14242..14835)
                     /gene="clpP"
                     /locus_tag="MB219_000891"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCC8426624.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15015..15542
                     /locus_tag="MB219_000892"
     CDS             15015..15542
                     /locus_tag="MB219_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426625.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15633..16370
                     /locus_tag="MB219_000893"
     CDS             15633..16370
                     /locus_tag="MB219_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCC8426626.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16374..17291
                     /gene="miaA"
                     /locus_tag="MB219_000894"
     CDS             16374..17291
                     /gene="miaA"
                     /locus_tag="MB219_000894"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCC8426627.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17293..18522
                     /gene="hflX"
                     /locus_tag="MB219_000895"
     CDS             17293..18522
                     /gene="hflX"
                     /locus_tag="MB219_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCC8426628.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5992 a         3209 c         4029 g         5308 t
ORIGIN      
        1 attaccgcag atgcaagctt ttgaacaccg ttttatttgt ctcacaccct tttccttcct
       61 acttgtaacc ttgctcccgt gttagaaaca tgttacacta aatatgtgaa attattaaca
      121 aggagcgtcg ttatgcgaga aaaaacagat acactgccaa aagcaactgc gaaaagatta
      181 cctttatatt tacgctactt gaagatgctg gatgactcag gtataagtag aatcaaatca
      241 aatgagttca gcgaaatcac ccaaattcct tctgcaacga tccgcagaga cttctcgcaa
      301 ttaggcgagc taggcagaag cggatatggc tatgatgtcc cttttcttat cgatgtattc
      361 aataatatcc ttaatactaa agaagaaaaa cgaatcgcat tagtaggata tggaaatctt
      421 ggcaaagcat tgaaacacaa caattttaga agaaatgaaa atctaaatat cgtgtgcgtc
      481 tttgataatg atccagcact gatcaatcga gtaattgatg gagaaatgat ctatccgatt
      541 gatcgtttcg cggagatagc caaagcaaaa aatgtgacag tagcaatctc tacggtacca
      601 agtaaatatt cacaatctgc tattgatgaa attgtcaaag gaaatgtaac cgctatactt
      661 aattttgcgc ctgacagagt aactgtccca gcttatgtca atgttcaata cattgatttg
      721 acaaccgagt tgcagacgct tatttacttt gatgagaatt actcagaagt tttttcttag
      781 gaaaatttga gtaggcttat tgatcttgcg agaactcgtc gtaattcttt caaaaatttt
      841 ttagtactaa atgaagtggt tttttcacga ccacttgttg actaaaaaaa aaaaccattg
      901 tagaatgaag cataacgctg aacaagagga gtaactggca cattcattct tagagagaga
      961 actatctgct gaaaggttct caatgacaac cagtgaaggt agcttgggag tttctgtctt
     1021 gaatggagta gagatagacg agtcgtgatc gttaactcac ttgagaggca ataatgattg
     1081 attattgcaa tttaggtggt accgcgtaaa tcatacgtcc tagaatacga aagtatctta
     1141 ggacgttttt tagtttaaag gaggaaaaca gcatgacgga agaaaataac ctatcaacaa
     1201 aatataatcc gcaagaagtt gaagctggac gttatcagca ttggttagaa caagatttgt
     1261 ttaaaccgag cggagataaa aaagcaaaac cttattcaat cgtaatcccg cccccaaatg
     1321 ttactggaaa acttcattta gggcatgctt gggatacgac cttgcaagat atgctgattc
     1381 gtcaaaagag aatgcaagga tatgatactt tatggctgcc tggtatggac catgcaggaa
     1441 tcgcgacaca agcaaaagta gaagcgaagc ttagagagca agggatttct cgttatgatc
     1501 taggtcgtga aaagttcatc gatcaagtgt gggaatggaa agaagagtat gcgagccaca
     1561 tccgcgaaca gtgggctaaa atgggcttat ctttagatta cagtagggaa cgttttacat
     1621 tagatgatgg attatctgaa gcagtacgta aagtttttgt taccttatac gaaaaaggtt
     1681 tgatttatcg tggtgaatat atcatcaact gggatcctca agcacgaaca gctctatctg
     1741 atatcgaagt tattcataaa gatatcgaag gcgcctttta tcatatgagc tatgaactag
     1801 cagatggatc aggcgtagta gaaattgcga caacccgtcc agaaacgatg ttgggagata
     1861 cggcgattgc tgttcatcca gaagacgagc gttatcaagc gctcatcggc aaaaaagtga
     1921 ttctaccttt agtcaataaa caaatcccaa tcattgcaga tgaatatgta gatatggaat
     1981 tcgggacagg tgtagtaaaa atcactccag cacatgatcc aaacgacttt gaggtaggta
     2041 atcgtcatga tcttccacgc gtaaatgtta tggatgatga tggtacgatg aatgaactag
     2101 caggaaaata tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg caagatctga
     2161 aagaaatcgg tcgattgatt aaaatcgata cgatgatcca cagtgtgggt cattccgagc
     2221 ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt
     2281 tagcgaaaaa agccattgat aaccaagaaa cagatcaagc agtggaattc ttccctccaa
     2341 gattcaatca aacgttttta cgttggatgg aaaatgtcca tgattgggta atctcacgac
     2401 aattatggtg gggacatcaa attcctgcat ggtatcacaa agaaacaggt gaaatgtatg
     2461 tagggatgga agcaccagcg gatagtgaaa actgggtaca agatcctgat gttttagaca
     2521 cttggtttag ttcagctttg tggcctttct caacaatggg ctggccagat acggaaagcg
     2581 aagattataa gcgatacttc cctactagca ctttagtaac cggttatgac atcattttct
     2641 tctgggtaag cagaatgatt ttccaaagct tggaatttac tggcgaacgt ccatttgaga
     2701 atgtcttgat tcatggattg attcgtgacg aacaagggcg gaagatgagt aaatcattag
     2761 gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg agcagatgct ctccgctggt
     2821 tcttatcgaa tggttctgca ccaggacaag atgtccgctt cagctatgaa aaaatggatg
     2881 cttcttggaa ctttatcaac aaaatctgga atgcttcacg tttcgttatc atgaatatcg
     2941 aaggaatgac cgtagaagag atcgatttat ctggagaaaa atcagtggct gaccggtgga
     3001 tccttactcg tttgaatgaa acgatcgaac gagtaactga gctatttgat cgttttgaat
     3061 ttggtgaagc aggtcgacaa ctgtataact ttatctggga tgatttttgt gactggtaca
     3121 tcgaaatgag taaagaagta ctttatggag atgatgctgt ttcaaaacaa accacccgta
     3181 gcatcttagt ccatacactt gatcagatcc tacgtttatt gcatccaatc atgccattcg
     3241 ttacagaaga aatatgggaa catattcctc atcagggaaa ttctctagta gtggcagaat
     3301 atcctgttgt acgtccagag tttgatgatg aaacagcttc taaagggatg gaagtattga
     3361 aagaattgat tcgagccgtt cgaaatattc gttcagaagt gaatacacca ttgtctaaac
     3421 caattacgtt gctaattaaa acaaacgatg ctaaaatcga tcaattttta gttgaaaata
     3481 caagctatat cgaacgcttc tgtaatcccg aagagttgac gatatcaagt gagatcactg
     3541 cgccagatct agcgatgtca gctgttctga caggtgcaga aatcttcttg ccgttagcag
     3601 gattgatcaa tattgaagaa gaaatcaaac gattagaaaa agaactggaa aagtggacaa
     3661 gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg
     3721 aagaagtagt agaggcagag cgtgcaaaag aaaaagatta tttagagaag caaacggctg
     3781 tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa
     3841 atcagctagc tattactaaa agctagctga ttgaacatcg ttacaggaaa gagtttgagc
     3901 ctcactctta gcttcgattc ataaggagat gtgacataaa ttttgtcgca tctctttttt
     3961 agtctactct tattatgtag gagaaggagg gaggtggatc atgtatcaag agtcaataaa
     4021 gtagttgata aaatcgttcc tctctctgtt tcttttctct ttgtcatact tttatttttc
     4081 ttatgtgttc tatttgttat aataaaagag aaatggggga attgttgatg acgattgaag
     4141 aagcaataga atggatccat agtcgattac catttggttc acgtcctggc cttgaccgag
     4201 tcgaagcttt attagaagtg gtagggcatc cagagagaaa agtaaaaacg atccatatcg
     4261 ctggaacaaa cggaaaaggt tctactgtag cttatttgag aagcctatta gaagaggctg
     4321 ggctgacagt aggtacattt acttcacctt atatcgaaac gttcaatgaa cggatcgcaa
     4381 tcaatggcaa aagcattcca gacaatgatt tgatccagct tgtaaaaaaa tatcagccac
     4441 tgatcaaaca acttgatcaa tttgaagcag tagctggtat tactgaattc gagacattaa
     4501 cagcaatggc atttgattat tttttgatga agaaagtgga cgtagcgatt atcgaagttg
     4561 gcttaggcgg cttattggat tctaccaatg tagctgtacc aatgctgacg ggaataacaa
     4621 cgattggact tgatcatacc gaaatacttg gagatacaat tgaagaaatc gctgcacaaa
     4681 aagctggaat catcaaacaa ggcgttccag tagtaacagg aaatattgcc ccagaagctt
     4741 ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta tacggggaaa
     4801 attataaaat aaactatctg catcctgatg agatgtgggg agaagtattt gaatttttta
     4861 atgaaatagg aaagatccct caattaacca ctccgctttt agggcaacat caagctgaaa
     4921 atgcaggagt agccatcgaa ttgtattttg tttactgtca attggaaaag ctgcctttcc
     4981 gagaaaaaga tgttttgaag ggattgaaga aatctcgatg gccaggaaga atggagcgtt
     5041 tgagtaacga gccattagtt gttttagatg gtgcccataa tacgcatgca atgaaccggc
     5101 ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat caacattttg ttttccgcct
     5161 tacgaactaa aaatgttaga gaaatgctag aacgattgca gcaagtacca aatgtccata
     5221 tttatttgac gacctttgaa catccaaaag ccatcgaatt aagtgaagta tcagatttag
     5281 caagtgagag agtatcaatc gtttcattgt ggcaattcgg attagcagat atattggaaa
     5341 agatgacaaa tgaagacgta ttagttgtga ccggttcact ttatttcatt tcagaagttc
     5401 gccatttatt aatacaatta ggaggagatc atgaagtata atgcagtgat ttttgacatg
     5461 gatggtttgc tgttcgacac agagttagtg tattacgaag ccacacaagt agtagcagac
     5521 aagatgggtt ttccttatga taaagacctg tatttaaaat tcttaggtgt ttcagatgaa
     5581 gaagtatggg cgaattatca tatgattttc tcggaatttg gcaaaacaag tgtacaaaga
     5641 ttcatagatg attcatacca ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg
     5701 aagcccggtg tgttggaact cctctctttt ttagaagaac atcagattcc caaagttgtc
     5761 gcttccagta accaacgagc agtcattgaa cttttattga agaagaacaa tctgtttgaa
     5821 cgatttgaga cgattgtttc tgcagaaaat gttcaacgag caaaaccaga cccagaaatt
     5881 ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag aaacattgat tttagaagat
     5941 tcacaaaatg gtattttagc agcttctgcg gctgagatcc cggtagtcat ggtaccagat
     6001 ctgcttccac catcccaaga attgatcaaa aaaacagctg gggttgtttc ttctttacat
     6061 gaaatccctg cattgttaaa ataaaaatcc atctcttttc gtatcttatt ttagataaga
     6121 tagaaaggag atgttttttt gggaaaacga ttgatcaaag aagtacctac tagctcttta
     6181 ccaagggaac gtatggaaat ttatggcgcg gaagctttat cggatcagga gcttttggca
     6241 atcttactgc gtaccggtca acacccttat agtgtgatgt cgattgctgg aaatctgtta
     6301 aaaacgtttg gaggactggc ctctttacga caggctactt tgcatgagtt agaagagatt
     6361 cgagggatag gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga
     6421 attcagacgg atcatgaaag agcggctcca ccggttcgta ccagctacga attggcccag
     6481 caattgattc tggaaatgaa agatcacaaa caagaacatt tggtttgtat ttatttggac
     6541 acgaagaatc aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc
     6601 gctcatccta gagaaatttt ccattatgct gtacgttatt gtgcagcaag gatcgtttta
     6661 gcgcataatc atccttctgg taatgttatt ccatcgcagc aggatatgaa ttttaccaaa
     6721 aggatacaaa agtgtggaga aatgatgggg atcactgtac tggaccactt gatcatcgga
     6781 cgcaaacgat attttagttt gagagaagaa ggaatgatgg aagagaagta agttgctttc
     6841 ttgcaaaaaa aatgaagcaa gtgtacaatt atattgtaat tttgtttggg aatagaagtt
     6901 gaaacatctg ttgcacctct atcgtacacg agcaatgtta caatttaaat tagtgctttt
     6961 ggcacgagga ggatattatt atggaaaacg gaacagtaaa atggttcaac gctgaaaaag
     7021 gatttggatt tatctcacgt gaagatggaa gcgacgtatt cgttcacttc tcagctatcc
     7081 aaggtgacgg attcaaaaca ttagaagaag gtcaagcagt gactttcgac gttgaagaat
     7141 cagaccgcgg cccacaagct gcaaacgttg taaaagctta attttatctg taaatcaaat
     7201 tgatggtaga catcaatttt tacgagagaa caaagcgagt aacaaaaata cctgccacaa
     7261 gagacaattt ctcttgtggc aggtattttt ttgtatgaaa aagtaaataa atttaagagg
     7321 ctatgaaaga gccaccttgt atcaaggaat aagaacaaac aaaaatggct tctcatgttt
     7381 ttcatttgtt ttctcttata ttcaggacaa ggtgctgatt tcataacctc ttatttattc
     7441 ttcatctggt gtcattacca ttggcaaaat cattggatgt ctttctgtac gttcgaataa
     7501 gaaaggctga agcgcgccag tcatcgcttc tttcaattta tattctgaac agtcaggtcc
     7561 attcaaacat tctcttaaag cgtggaataa gattcgctga gcttcattga tcatttctcc
     7621 tgattctcgc atgtagacaa atccacgaga gagaatatct ggaccagcca agatttcttt
     7681 tttcttgata tccactgtcg cgacagctaa tacaagacct tcttctgaaa gcaggtgacg
     7741 gtcacgtaaa acgacattac caatatcacc gatcccgcta ccatcgatat acacatctcc
     7801 agccacaaaa tgcccagcag gacgcgcaga atcgctagtt aacgctaaaa catcgccatt
     7861 ttccatgatg aagcagtttt ctgcaggaac acctacatct tgtgctagag aagcatgtac
     7921 tttcaacatg cggaattctc cgtggacagg aataaaatat tttggcttca tcaaacgaag
     7981 cattaatttt tgttcttctt gtccaccgtg accggaagta tggatgttat tgatttttcc
     8041 gtgaatcact tctgcaccag cttcagacag caaattgatc aaatgattga cacttgttgt
     8101 attaccagga ataggtgaac ttgaaaagat cactgtatcg tctggctgga tttggatttg
     8161 acggtgagta ccattcgcga tgcggctcaa cgcggccatc gcttctcctt gagaacctgt
     8221 acataggatc atcgtttcat tggcaggcaa gcgattgatt tcgtgtgcat ctacaaatgt
     8281 gccgtctgga actttgatat atcctaaacg ttgaccgttg acgatggcat tttccatgct
     8341 tcgtccgaaa acagcgattt tacgaccagt tgccacagcg gcatctgctg cttgctgcaa
     8401 acggaagatg ttagaagcaa agctggcaaa aatgatacgt ccttcgattt ttgaaaagat
     8461 cttcaaaata gaatgcccaa tcgttttttc tgatttagta aatgttggaa tctcagcatt
     8521 tgtactatca gataaaaggc acaagactcc ttcttccccg atacgagcca ttttatgcaa
     8581 gtttgctggt tcgccaacag gagtgaaatc gaatttgaag tctccagtcg ctacgatatt
     8641 tccagaaggt gttttcacca cgatccctaa tgcatctggg atactatggg tcgttctgaa
     8701 aaagctgaca gaagtctttc taaaacggat gatcgtatct tcattgatct catggagctc
     8761 tgcatctcga agcaagccat gctcatctaa tttgtttgtg atcaacgcta atgcgagagg
     8821 accagcatag ataggaatat ttgcttgttt cagcaagtag ggtattccgc cgatgtggtc
     8881 ttcgtgcccg tgagtaatca ccaaggcttt tactttgtgt aagttttgta tgatgtagct
     8941 gtagtcggga atgacatagt caattccaag taaatcgtct tccgggaatt tgatcccggc
     9001 atcaatcaaa atgatttcat cttggaattg taccccatac gtatttttcc cgatttcacc
     9061 taatccacca atagcaaaga cgcctgtctc gttatttttg atggaaagtt tcatattaaa
     9121 actccactaa tgagaaatca gcattttctt tttcgtactc tagatgactt ccttctagaa
     9181 gttgaacgaa ttcaatattg tatggggtat tttcttccac acgttggcga gcgtctacat
     9241 cgctttttgc ctcaatgtaa agagattttg tttcttcccg tttagggttg cgtacttttg
     9301 tttcttgata ataaacttta tagatcattg ttttgtctcc tttatctgcg taaagcagtg
     9361 atttagctag ttgatttttt tagacaacac aaaacgaggg gacaagccac ctggtttttc
     9421 atatgctttg actagaatac tggattcgag tcaaagagac aagcctcgtt tttgcttatc
     9481 tgttcggata ttttttcaac cgtaccttct gtataaaaaa tatacaacaa ttaattgtta
     9541 ttctagcata aaacagcaaa taagtataga aatcaactag attgaaagaa aaaatcaatg
     9601 tttatcatcc cgctataatc ataatgactt ttttatgaga gaagtgttct attaataggc
     9661 tcgttgtgct atcattttta gtaatgaaaa tgtaacgaaa gattaaaagg agatgagaaa
     9721 gtgaaactaa tgtggcatta taccatgcgt tataagaaat tcttgttttt caatttcata
     9781 tgcgtatttg gttttatttt gattgaatta ggacttccta cgattttagc acgtatgatc
     9841 gatgtaggaa tccgcaatga tgattttgat tatgtgaagc aacaagggat atttatgatc
     9901 gttattactg taatcggtat tattttgaat atcttgttag gttattttgg ttcgaggatt
     9961 acgactaata tcgtagcaga tattcgagat gatttgttcg aaaagatcca aagttattcg
    10021 catcaggaat acgaaaatct aggcgtatct tcattgatca ctcgtacaac aaacgatgct
    10081 tatcagatca tgttgttcct gcaaaatatt ttacgtatcg gttttatggc accaatgatg
    10141 ttcattgtca gtttgtacat ggtcatgcgt acaagtgtga cactcagttt gtatgtagta
    10201 ggtgcattac ctttactatt attggcagtc gtagcgattg ctaaggtgtc tgaaccgtta
    10261 tctaaaaagc aacaaaaaaa cttagataaa atcaacagta tcttgcggga aaatctttca
    10321 ggattgcgtg tgattcgtgc attcgtcaat gaaaagtttg aagaaaaacg ttttgctgta
    10381 gtcaatgaga attatgcttc tagttcaaaa agcttattcc gtttgatggc tgttgcacaa
    10441 ccgggttttt atttcttatt taatatcgta atggtattga tcatctggag cggggcagtg
    10501 caaatcgatg caggcgagct tcgtgtagga gatttgattg cgtttatcga gtatatattc
    10561 catgccttgt tctcattcat gttatttgct agtgtgttca tgatgtatcc aagagcagct
    10621 gtttcagctg gacgtatcca agaagcccta gatgccaaac cttcgattgt agaagatcca
    10681 aatggtatat ctgatacaaa aactaaaggg tatatcgaat ttcaaaatgt aacatttgct
    10741 tatccaggcc attcgcaaga gccagtgata cgaaatgtca gttttacagc ctctccagga
    10801 gagaccgttg cttttattgg aagtaccggt agcggaaaat ctacgttgat ccaattgatc
    10861 cctcgctttt atgatgtgag tgaaggaaag atcttgatcg atggagtaga cgtgagagat
    10921 tatcggctaa gcaagttgcg agataagatt gggtttattc cgcaaaaagc gctgctattt
    10981 actggaacga ttgcagataa tttgcgatac gggaaagaag atgctacgca agaagaaatg
    11041 gaacgtgcag ctgagattgc tcaagcttca gaattcattt ctcgaaaacc agatggattt
    11101 aatgaacatt tatcagaagg tggttccaat ttttcaggtg gacaaaaaca acgattggca
    11161 atagccagag ccatcatacg ccgtccggaa atctatattt ttgatgacag cttttctgca
    11221 ttagattatc aaacggatgc gaagttacgt gctcgtctga aaaaagaaac gacagaatct
    11281 actgtattga tcgtagcaca acgtgtggga acgatcatgc atgcagataa gatcatcgta
    11341 ttaaatgaag gagaagtcgt cggtatgggg acgcatcggg agttgttgga aaaatgcccg
    11401 atttattatg atattgccgc ttctcaatta tcaaaggagg aattagcatg aaaaaaacct
    11461 tttcttcctt aaaacgttta gctcgatata tcagaccgta tcgtgagact tttcttttag
    11521 tgatcctatt tacagttctg acagtggctt tcaacgtggc tttgccatat gttacaggac
    11581 ttcctactac agaaatcagc cgtaatattg ccaatggcga atcaatcaat ttttcttata
    11641 tattcaaatg tctcatttgg atcacagttg ttggaatagg gtattgtatt tcgcaattac
    11701 tttctggtgt tttgatgaca aatgtcgttc aaagtgccat gcatgatttg agaagagaca
    11761 tagaagaaaa aatcaatcgt cttccggttt catatttcga taaaaaccag caaggaaata
    11821 ttttgtctcg tgtgacgaat gatgtagatg cagtcagcgg tgctttacaa caagctttta
    11881 ttggtatcgt aaatgcgata ttgggaatta cgttggctgc agcaatgatg ttttatatcc
    11941 agccgatgat ggcgctgatc tctatgatca tgattccaac atcgatctgg atctcaaaac
    12001 gagtgatcaa tgcttcgcag aaatattttc aaagcatgca gaattctctt ggtgagctaa
    12061 acggctacgt ccaagaaaat atgacaggtt ttagtgtatt gaaagtatat ggacgcgaaa
    12121 aagaaacatt ggaaggattc aaaaaagtta atcacagctt gaaatacttt gggtttcgtg
    12181 cagcattcat ttctggcttg atgatgcctc tagttcaatt aactgcctat gctacttata
    12241 ttggtatggc tgttctagga agcttttatg ttattactgg ggcgatcgtt gtcggtcaac
    12301 tacaagcatt catccaatat atttggcaaa tcagtcagcc aatggggaac gtgacacaat
    12361 tgtcttcagt tttgcaaagt gcatcagcgg caacgaaacg tgtattcgag attttagatg
    12421 agccagaaga aaaagaaaac aaagtagatg cttcactgcc agaaacgatt ttaggagatg
    12481 tgacattcga tcatgtcgat tttgcatatg atccgaagaa accattaatc caagatttga
    12541 attttgaagt gaaagctggt caaaccgtag ctgtggtcgg tccgaccggt gccggcaaga
    12601 cgacattgat caatctattg atgcgttttt atgacgtaga caaaggagca ataaggattg
    12661 atggaatcga caccaaatca atgagtcgca gtgatgtgcg ttcgctattt ggtatggtct
    12721 tgcaagatgc atggctttat caaggaacga ttgcagataa tatccgcttc ggaaagcttg
    12781 atgcgacaga ttatgaagta gttgatgctg cgaaaacagc aaacgtagat cattttatcc
    12841 gaacgatgcc agatggatat gatatggcca tcaattctga gggagacaat gtgtctttag
    12901 gacaaaaaca gttgctgacg attgcccgag cagtggtctc cgatcctaaa atcttgattt
    12961 tagatgaagc aacaagttca gtagacaccc gtttagaagc gttgattcaa aaagcaatgg
    13021 atcgcgtgat ggagggacgg acaagtttcg tcatcgctca ccgtttatcg acaatccgtg
    13081 atgcagatct gattctagtg atggatcaag gtcagatcat tgaaaaagga acgcatgaga
    13141 gtttattggc acaaggtgga ttttatgaaa aactgtataa cagccaattt gctgaggaag
    13201 cagaataagt catataagaa taaaacagtt taaaacttat tttttgttct ctattcttga
    13261 taatggtaaa gtaaagttat caagaaaagt gaggacacgg agatggaaca gtttttaaat
    13321 gaaaaaatta ctgaagccaa aagagaagtt gatcgtttaa cgcacgtctg tcagacgaag
    13381 ccttttgatt cagctgattt ttttgaaaat gagtttcaaa tcgaacgctt ggcagcacaa
    13441 atagaagtgt acgaattaat tttaaaaaaa gtaatagaag aaaatataga tgtacaataa
    13501 aaacaggtaa aacgtcagtt accagatact gtaaatagac atctggcctg agcaggtttt
    13561 acctgttttt tttcttatga aataaatata tgctatgata cctttattca ataggattag
    13621 tttctacaaa aaatgaaagg agaaaaataa atatggaacg gtttatagca tcaaatgcca
    13681 cagtgattgg tgatgtgaca ttgtcagaag atgtgacgat ctggtatcaa gctgttttac
    13741 gcggggacag taattggata aaaataggac aaagaacgaa tatccaagat ggtacaatca
    13801 tccatgtcga tcatgatgca cctgtagata ttgcggagaa tgtgacggta ggacatcagt
    13861 gcatgctaca tggctgtaca atcgaaaaag gggcgctgat cgggatgggg accacgatat
    13921 tgaatcatgc ggttattggt gagaatagtt tgattggtgc tggatcatta gtaacagaag
    13981 gaaaagttat tcctcctaat gtcttggctt ttggtcgccc agctaaagtg atccgtcctt
    14041 tgacaaaaga agaaatacaa aaaaacaaag aaaacatcca gcattatgtc gagataggtc
    14101 ggatgcatgc gcaaggaaaa ttcaaggagc aaagataggc agtagcatcc ataaaataaa
    14161 aaaggatggt cagtttgagt tgaccatcct tttttatttg gtttagataa gagagcttaa
    14221 aaaagcttcc tttgaaacag attaatttaa tgaagaactg ttttccatga cttcatcgat
    14281 caatccatat gcttttgctt gttcagctgt catatagttg tcacgatctg tatctttttc
    14341 aatcacttca agcggttgtc cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt
    14401 ttgcaaaata tggcgtgccg caatttcgat ttcagttgct tgcccttgag cgccgccaag
    14461 aggttgatgg atcatgatct cagcatttgg taaagcaaat cgtttacctt ttgtaccagc
    14521 tgtcagcaag aagcttccca ttgaagcagc catacccata acaattgttt ggacatctgc
    14581 tttgacaaag ttcattgtgt cgtaaatagc catacctgct gtaactgaac caccaggaga
    14641 attgatataa agatagatat ctttttcaga atcttgagcg tctaagaaaa gcaattgggc
    14701 gatgatcgaa ttagcaagat catctgttac ttggccgctt aacatgatga tacggtcttt
    14761 caataaacgt gagtagatat cgtaagctct ttcacctcgt gaagattgtt caatgactgt
    14821 aggaattaaa ttcataatag agttcctcct tgatttcgtt tagaaatagt gtaccataat
    14881 ttagtttcta atacctaaaa tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa
    14941 cttaggcaaa ttagtcgcat aaacccgctt tatggtatga taagcagaga aacaaaacaa
    15001 ggaggaacaa cagattgata gttggtatta ctggccttat cggagtgctg acaggtgctg
    15061 tagttgtcta tatcggcatg atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta
    15121 gacgtgaatt ggaattgaaa gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga
    15181 tcaacgagat cttgcaaaaa cggacaacgc tgatggaaga atatgtcagc tttgatgcat
    15241 ttgatgattg ttatatcacg attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa
    15301 atagttttta tctgccaaat tatattttag acgagctatt taagaatatc tcccatcgaa
    15361 aagtgatttt atctccagat gaaacaatga agattggtgg atatgcttat aaaggcggac
    15421 gcgtgatttt agagaatttt acggatagtc tgactgaaat gatcaatgaa aaaaaagctc
    15481 agttgaagac tatgacgcgc cagccgattg gtttcttctc gaaagatgat gtttggagct
    15541 gatattttga agcattgccg ttgcacatga gagcgtggta atgctttctt tttttcttaa
    15601 tgatacaata gaactgaacg gagtgaagaa agatgttaca aatctatgcc catcgcggaa
    15661 gcagcggcac ccacccagaa aatacgcttc ctgcatttgc agaagctgta cgagtaggtg
    15721 cagatggtat cgaactagat gtccatctaa gcaaagatgg ttatttgatc gttatgcacg
    15781 acgaagaagt ggaccggacg actaacggta aagggctgat tcgagaaaaa acattggaag
    15841 aactcaaaaa attgaatgca gggggcaaat tcagcaaaga atttccagca gctaaagttc
    15901 ctgaattgag agaagtgtta ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga
    15961 ttgaaattaa aactgatcag tatgaatatc ccggtatcga agaaaaatta gccgaaacat
    16021 taaaaagtag agattggcct tttcaacatg catattgcag ctttcatttg gattctttgg
    16081 aaaaaatcgc attactggaa ccggagatac agctggactt attgatgagt acttctgata
    16141 aaaagatcca actagcagca gaaacccctt atatagaagg agtacatcca aaaaatactt
    16201 ggttgttcga acatccagtc caggcagctg attttccaaa agcaattcgt gcatggacga
    16261 ttaacgatga agaagaaatg gccaaatgtc aagaactcaa aatagatgga ataataactg
    16321 attttccgga gaaagctctt cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa
    16381 aagtattagt gatcgtcggt ccgacagcag tcggtaaaac agcgctaagt gtggaactcg
    16441 cacaactatt tggaggagag atcatcagtg gtgattcatt gcaaatctat aaaaatctgg
    16501 atatcggtac agcaaaaatc aaagaagaag agatgaataa tgttccgcat catttgattg
    16561 atgtgatcga acctactcaa cgttactcag ccgctgattt tcagcattca ggtcgacaat
    16621 tgattaccga gattgcgaac cgagggcacc tacctatcat agctggtggt acgggtctct
    16681 atatccaatc ccttctatat gattatcagt taggagcgac agaagaagcg aatcctgaga
    16741 ttagaaaaaa gtatgaaacg ttagctgaaa caattggttc ccaagcatta tggaatcagt
    16801 tgaaagaaaa agacccggca gctgctgaga aaatccactg gaacaatcaa cgcaaagtga
    16861 taagagctct ggaagtttta gagacgacag gacatagtat cacggcacct aaagaagagc
    16921 caaagcaatt gtatgattac ctcatgattg gattagatac gaagcgtagt attctttatg
    16981 aacgaatcaa tcaaagagtc gatttgatgc tagcagaagg tttagtagca gaagcgcgta
    17041 aggtgtatga gcttggagaa gtccaggcca gtcaaggtat tggatataaa gaatttttcc
    17101 cttattttga agggaaagaa acattggaag aagcaacaga acaagtcaaa ctacattcga
    17161 gaagatacgc gaaacgtcag ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg
    17221 atcttataag tgagccaaat caaaaaagtc agattgagca aacaatcaaa aaatggctgg
    17281 aggcggaata aaatggaaga aaaagtgatc atcgtagggg tagaaaccga agcaaaccag
    17341 cgttactttt cagagtcaat ggaagagcta gttcagctga cgaataccgc atcaggagaa
    17401 gtcgtattca caattacaca aaagagacca caagtggatc ggcaaacgat tatcggtaaa
    17461 gggaaattac aagaattagt tcaacaagcg gatgcgcatg aagcggattt gatcatcttt
    17521 aatcatgagc taactccgag acagagccaa ctgattaccg atgctgttgg tcttccggta
    17581 attgatcgcg tgcagcttat cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg
    17641 aagctgcagg ttgaacttgc tcagttagaa tacctgctgc ctcgtttagt agggcaaggg
    17701 aaaacattat ctcgtcttgg cggtgggatc ggaacaagag ggccaggtga aacaaagtta
    17761 gaaacagacc gccgacatat acggaataaa atttttgctg ttaagaaaga attgaaagaa
    17821 gtagaagctc atcgagagcg caatcgccaa aaaagaaaaa atagcgagat ttttcagatt
    17881 ggacttattg gctacacgaa tgccgggaag tcaactatct tgaatctatt gacacaagcg
    17941 gatacttatt cgaaagatca attgtttgca acgttagatc cattgactaa gagatggcgt
    18001 tttgctgaag gatttgagat tactgtaacg gataccgtcg ggttcatcca agacctgcct
    18061 acgcaactga tcgatgcttt ccattctaca ttagaagaaa gtcaaaatat ggatcttttg
    18121 ctacatgtag tcgatgctag ttctccagat cgtattttac aggagcagac tgtcttaaaa
    18181 ttgatggatg aactgaatat gaaagaaatg ccgatactga ctgtttacaa taaggcagac
    18241 cagatcgacc cagcgatgtt cacgcctacg ctattcccaa atgtattgat ttctactcag
    18301 acgaaagaag gtaagatggc gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg
    18361 accccttata cactttctgt gccaagcaac gaaggacaga aattaagcga gttgagaaga
    18421 caaacaatgg ttttggacga agcgtacatg gaagaaacaa acgaatatga agttaaaggg
    18481 tttgcaaaaa ccaattcaaa atggttaaga aaaaaccaat aaaattttat ttttttaa
//
LOCUS       DAHEYX010000038        17988 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHEYX010000038 DAHEYX010000000
VERSION     DAHEYX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.038"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            218..502
                     /locus_tag="MB219_000896"
     CDS             218..502
                     /locus_tag="MB219_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCC8426629.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(783..1202)
                     /locus_tag="MB219_000897"
     CDS             complement(783..1202)
                     /locus_tag="MB219_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCC8426630.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1204..1788)
                     /locus_tag="MB219_000898"
     CDS             complement(1204..1788)
                     /locus_tag="MB219_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCC8426631.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1835..3310)
                     /locus_tag="MB219_000899"
     CDS             complement(1835..3310)
                     /locus_tag="MB219_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCC8426632.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3491..3802
                     /locus_tag="MB219_000900"
     CDS             3491..3802
                     /locus_tag="MB219_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426633.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3917..6253)
                     /locus_tag="MB219_000901"
     CDS             complement(3917..6253)
                     /locus_tag="MB219_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8426634.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6395..7063
                     /locus_tag="MB219_000902"
     CDS             6395..7063
                     /locus_tag="MB219_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426635.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7066..8109
                     /locus_tag="MB219_000903"
     CDS             7066..8109
                     /locus_tag="MB219_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCC8426636.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8346..9227)
                     /locus_tag="MB219_000904"
     CDS             complement(8346..9227)
                     /locus_tag="MB219_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCC8426637.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9386..10114
                     /locus_tag="MB219_000905"
     CDS             9386..10114
                     /locus_tag="MB219_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCC8426638.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10270..10587
                     /locus_tag="MB219_000906"
     CDS             10270..10587
                     /locus_tag="MB219_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCC8426639.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10580..11188
                     /locus_tag="MB219_000907"
     CDS             10580..11188
                     /locus_tag="MB219_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCC8426640.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11243..12034
                     /locus_tag="MB219_000908"
     CDS             11243..12034
                     /locus_tag="MB219_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCC8426641.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12172..12684
                     /locus_tag="MB219_000909"
     CDS             12172..12684
                     /locus_tag="MB219_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCC8426642.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12710..13582
                     /locus_tag="MB219_000910"
     CDS             12710..13582
                     /locus_tag="MB219_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCC8426643.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13758..14120
                     /locus_tag="MB219_000911"
     CDS             13758..14120
                     /locus_tag="MB219_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCC8426644.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14129..14881
                     /locus_tag="MB219_000912"
     CDS             14129..14881
                     /locus_tag="MB219_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426645.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14895..16697
                     /locus_tag="MB219_000913"
     CDS             14895..16697
                     /locus_tag="MB219_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426646.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16974..>17988
                     /locus_tag="MB219_000914"
     CDS             16974..>17988
                     /locus_tag="MB219_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCC8426647.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVT"
BASE COUNT         5630 a         3131 c         3719 g         5508 t
ORIGIN      
        1 tttaaggaag aggttgtgag cctgccgttt aactctgagt cacaaggagc aatttcaaaa
       61 aacagcttct catgtttgtt gactattgtg acttgtgacc gaggagttgg caggagaaca
      121 ccgtttattc gaaaaaagga actgcgacaa gacttttgtc acagttcctt cttttttttg
      181 ctatgctata ctagatgtaa aaaataggag aaataaaatg agggaaattt attcttttga
      241 atcaaaaatc tatgcttccg aagtcggcaa gggcggggca tatattattt ttccgtatga
      301 tataagaaaa gaattcggtg ctggaagagt taaagtccaa gcaacttttg acaatgtgcc
      361 gtatgaaggc agtatcgtga atatgggcgt gaaaaatcca gatggttcag tctgctacat
      421 ccttggacta cgaaaagata ttcgtaaaaa catcggaaaa gaaatcggtg atattgtagc
      481 agttacagtg aagcaaaaat aaaagaggtt gtgacaaaag acgtgtcaca acctcttgat
      541 aaacggtgtt caaaagccag tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa
      601 cccatgttta caaaattttt cttactactc gaaacgaagc ggctttatca caacctcttg
      661 ataaacggcg ttcaatcagc tgtccctcgg tattagctca aaaacggcaa aatatgagaa
      721 gctatttttc catttttttc tctcatactc aggtcataac gctgatttca caacctcttc
      781 tttcataatg gtttttcacc atctttataa taaactggtt ctttgatatg gaaatctatt
      841 ttttccaacc aatctaaaat cttgtaacct aactcttctg acatcggaaa agaggcgttg
      901 atttgtgcat tggagatatg gtcaacatgg ataccggaag tgatcgtaca acttcccgga
      961 acggcagttt tgatgatcac agctattttt tcggctaaga catcgtcttt atggaaccga
     1021 ccatcgtggc tagggaaacg tacagtttgc atctctgtat cacgagaaaa tgtagtgact
     1081 gttccgatat gtggtgtatc tccgcctgtt aaagtgatca ataagtcttt accgattact
     1141 ttaacagcag cttggatcgt gaaacctagt ttggttactt caactatcgt ttctttttcc
     1201 atattaatca ccctgccaac gttttgaatg ctcatttaca atttcaaatt gatccaaaag
     1261 tttcattgta tggtgattaa caagatcttg gatcgtttga ggctgattat aaaaagcagg
     1321 aattggtggt atgatttgca cacctagtcg tgctaattta gtcaagtttt ccaagtgaat
     1381 ggcatttaat ggcgtttcac gaggaacaat gactaacttt cgttgctcct tcattgttac
     1441 atcagcagca cgtgcaatca ggttgtcgcc taaaccaaca gagatacctg cgactgtttt
     1501 catacttgcc ggtacgatga tcattccatc ggtcaaaaat gatccgctgg cgacagcagc
     1561 acctaaatct ttgtttgaat aatggtaatc aaacaggctt tcgaattcag ttagtgataa
     1621 gtcggattcc agctttaagt tctgttttgc ccatggactg atgattccgt gtgtttctat
     1681 catcggatat tcttttagtt tcttcactag gttgatggca taaatggtgc ctgaagcgcc
     1741 acttacacca atcacgattt ttttcttccg ttgtgccaat tgttccattt tccgcactcc
     1801 tttttatgat gtcaaaacag ttgcagatca ttcattattt caaatatttt tcccaatctt
     1861 ctacttcttg gaattttgca cgtacaaaat gttctttcat atcgaaagga actgtaccgt
     1921 caaagatagt ttttgcactt atcccacgga aacgaataga gccaggatta tattctggta
     1981 attctgatgg gtccaatgga tggttcctca ttcctgggat cacgatgata tctttatctc
     2041 cttggaaacg agtgttgaaa gtccaaacta catcgttcat gtcgaaaata tcaacatctt
     2101 catcgactaa aatgactgtc ttcaattctt tgaaggctga taaagcaaga atagctgctt
     2161 gacgctgaat accttcatcc gcttcgtttt ctttacggat ttgcatgatg gtcatcaatt
     2221 taccaccacc tgcaggagga ttgtagacat ttactacttt tcctggaata gctttatcga
     2281 ccaaggcaag aatacttgct tctgtaggaa taccggccat agatacgtgt tcttcactgg
     2341 gaccaatcgt tgtttgcatg attggattgt cttttcggtg agtaactgct ttgactttca
     2401 ctacttgtac agctggatta gcatccccag tatagccagg aaattctggc attgcttttc
     2461 ctgagttcgt gttgatatct tcttgcattg tttcatttgg catgatttct gcttcaatga
     2521 tgaattcagc acgagcaata cccacttcat cgactgctat accatcaacg atttgaatag
     2581 gttcgttacg caaagctcct gcacaccata gttcattgta gccaagtgga gtggtaggtg
     2641 gttcgaaagt cgtgccaatg gcaattgctg gatctaaacc aatattgata gtgataggca
     2701 ttggtttatt caaggcttcg aattgttttt ggaagtgacc aatgtggcgt ccgccaggca
     2761 tgatgtacat accgatcgta tctttatctt ctagaaccat acgatggatc gttacatcgc
     2821 tcattgtttt ttctggatct gatcctaaga ctacacccat tgtgatgtat ggaccagcat
     2881 cttcaggagt atttgtaggt gcaggcaaaa tgttgcgaat atcgaagtct gcatcggtag
     2941 ctagatgaac gacttcttgt gctggagcgt ctgctttgtc tactttcaca ggtttgattg
     3001 gattttctac agcgtctttc aagaaacgac caagtgttcg ataatcatgg tgcaaaattt
     3061 cacctacacg tttacgacta gccattgttc cgatgttcac acgtgtgccg gggaaacctt
     3121 tgatattgtt gaaagtcata gctggtcctt cttgagtcgg acgttttact gtaccgccag
     3181 caccgatata acggtaaaca ccagataatt ctgcttcagg atcgatttca atatctgttt
     3241 cgtgatattg acctggaagt gtttttagtt cttcgatgac ttttcttaaa tcatacggtt
     3301 gatctgtcat gagagattcc tccttagttg tctttaccta tctatagtaa aaggtgaaaa
     3361 agaggaaaac aattcaactt gttcggtaaa gtattctaca atggaatgga tgcgtttttt
     3421 tgataataga ataaactatg atatacttat cactaaaaga aagcgcatac ttagtgttgg
     3481 aggaaggaaa gtgaaaaaaa tggaggagtc atttcctaaa gcagtaaaag tggaaaatat
     3541 agcgaatatt ttgaaagtta catttgaaaa cggagaagta aaatacgtga aatctcattg
     3601 gacagaagag ataaccgatg ccttgcaatt tggaaaaaaa ggaagaggaa aaagaaaaaa
     3661 tcttctcgca ctttctcgta atatgtggat tgggactgaa gtaacgattg aagcagatgg
     3721 aacagtattc ataaatggaa aagacaggta tacgccagaa gagttgtggt acaaaggaaa
     3781 gaaaagtata ccagaattat aaaaaataat taaaaaaaac agcgcgaaac aaggaacata
     3841 ttacttcctc gtttcttgct gttttctact atttcagcgc tcttatgact caaatctaaa
     3901 tgtggttgtc acaaccttat tctgcacgca aagcgatagc ggcgtctttt ttcgctgcca
     3961 ttcgtgcagg aagatgaccg ccaagcatcg tcaatatagt agagacgatc actagaataa
     4021 tcgcatggac aggatttagt tgagcaacat tttttagatc cgtcatgttg tataagatgc
     4081 tattgatagg gaacgtagct agccatgcaa taaacacacc taatatccca gaagaaatac
     4141 ctaaaataca cgtttctgcg tcaaatacgc gagtgatgtc ttttttacgt gcacctaaag
     4201 cttttagtac accgatttct tttgtccgtt caatcacaga tgtataagta atgatactga
     4261 tcatgatcat acttgttact aaagagatcc ctgcaaaagc aataaggaca taagtgatag
     4321 catccatcaa tccgccagtc agttcagtca tcgttccagc taaatcagta taaatgattt
     4381 tatcttcatc tgatttgcct ttattgtaat catctagata atccaagatc ttttctttgt
     4441 cttcaaaatt gttcggataa atcatgatac ttgaaggcaa gctgtctcca cctaaataac
     4501 taagcaatgt ttgtttggca ttttcgtcaa ctttttcagt agttaagaca tttacaccac
     4561 tgtctctttg agctttgacg atttctgatt ctttgttttc gttgacgatt tgagtcgtta
     4621 attgatcgct ataagcgatt cctggagata agagattcat tgtagaagaa gatttcacgc
     4681 gtaggatccc cgagatcttc aattcatcag aagcattctg atacatcgca tcataatctg
     4741 tattagggat aaaattccca gttggaagtt ttgtatagta agtattgttg tttactaatt
     4801 tgaatgttgt accaacgata tcatcaaaat caagtttttc gtcttctttc acatcaaatc
     4861 caagattctt caatgcgttg atattagtat tattattgcc gtctacgatc agcacgacat
     4921 ctgttgctga ggctggataa cttccggcaa gtaaagaata attgtctttt aaaaagtttt
     4981 ctttgctagt gtcaagctgt gtagggaaac ttgacactcc cacacctgtc atcgctgaca
     5041 tcgtactaga taaagaaaga gattccgtat caggattttg attcgagaag ctgacaggct
     5101 gtactttgcc atttacatca cgtaaaagat tgattcctgt agtacgggta aatccgatat
     5161 tattacttaa gttaggatcg atgtctgtta cgtaatcgac gtatgtttga tcaatcttat
     5221 tcgtgtgctg tgctttatct tcttcgctga cttttgcagt aatcgttttg ctgtctggat
     5281 aatccgcttt gcttgcaccc aatccggcat cgtcacgagt ctggttagtc gttaccttag
     5341 aaatagtaat cggaaatttc gccatcgttt cagcttgcgt gttatcgatt tgtttttgga
     5401 atccagaaga aagggacaga acgatcgcga ttccgatgat cccaatactg gaagcaaagg
     5461 aggtcaagaa agtacgtcct ttttttgttc ggatattgtt gaaggaaagc tttaacgctg
     5521 tccaaaaact catctttgtt ctgcgtaagt taaattgatc atcttttggt cgttcgatgt
     5581 gaggatggga atctgttaag atcttaccat cagaaaattc aatgattcga tctgcgtatt
     5641 gatgggccag ttcaggatta tgtgtgacca tgatcactaa tttttctttc gataactctt
     5701 ggatcaattc catgatctgg atacttgttt ctgtatctaa cgctccggtt ggttcatcgc
     5761 acaaaaggat atctgggtca ttggctagtg ctcgagcgat ggccactcgc tgcatttgtc
     5821 caccagataa ctgatttggc tttttatgca tatgatttgt caagccgact cggtgcaatg
     5881 catcttctgc ccgttttcgt ttttcgtctt tagaaacacc acttaatgtc atcccaagtt
     5941 caacgttttc aataatgcct agatgaccaa tcaagttgta actttgaaaa atgaaaccga
     6001 tagagttgtt tcggtaggca tcccagtcac tgtccttaaa gtctttagta gactttccgt
     6061 tgattaccat atcaccggaa tcataattat ctagtccacc aatcacattc agcatagtcg
     6121 ttttaccaga accactagga cctaaaatcg ctacgaattc tttttgtctg aaagcgacag
     6181 acacaccatc caatgctttg gtggtggttt cgccgacttt ataatatttt tttatatctt
     6241 ttaattgaag cataatcacc cttctcccgt attgaaatat tcggtatact aataagtata
     6301 tcagaacaat gatcgtagta tacgaaataa atgttaactg aagatgaact acaagaaaaa
     6361 atctagaaat ttttcaaaaa acagagggag atcaatgaat cgtattttag tagtagaaga
     6421 tgatccgcaa ttacagttgc tttaccgttc tgtgttggaa cgagcgggct tttcagtcat
     6481 caccgcattg aatggagagg atgcgctgaa aaaactagaa gcgacacgta tagaattaat
     6541 cattacagat atcatgatgc cgaaaatgga tggctatgac ttgctagact cattacgtag
     6601 caccagattt gacacacctg ttctaatgat tacggcaaag gccgattttg aggataagaa
     6661 acgcgggttc aaattaggag cagatgatta tatgacaaaa cccatcgatg tcaatgagat
     6721 gatcctacgt gtggaagcat tgcttcgacg ttcaaagatc aattacagca gtagcttgat
     6781 aatcggacaa acaaaattag agctagatgc ttatacagtg ttacaagatc agcattcgtt
     6841 gattctgcct caaaaagaat ttctgcttct gtataaatta ctgtcttatc ccaacaagat
     6901 cttcactcgc caacagttga tggatgatat ttggggatta gatacgaata cagaagaacg
     6961 aacgatcgat gtacatatca aacgtttaag gacccgtttt gaaaagacgg aagattttca
     7021 aatcatcaca gtacggggac taggctataa ggcggttatt taaagatgaa aaaaaagcta
     7081 ttttcacaat tatggatcta ttttgctagt attgtcttca tttcgatctt agtgacactg
     7141 ctgtgttttc tcggttttat tttcttattg tctcgacatt ctatctcgcc tgctgaatcg
     7201 gctggaaaac caacgttata cccgttattc gtctttgcgg ggttcagtat ggtcgtgggt
     7261 acaggaatat cgatttttgt tgggcggcgt atcctgcatc caatcagtgc gctagggaca
     7321 aatatgagtc tggtagcaac gggagatttt tcgattcgaa tggatgaaca gcagaaagta
     7381 gcagaagtac agcaactgta taaagatttc aacgttatgg tccaagaact caatagtatc
     7441 gagacactga ggaatgactt tgtgtccagt gtatcccatg aattcaaaac accactagca
     7501 acaatccaag gatatgttca attgttacaa gccccaaatc tctctgacga agaacgccaa
     7561 atatttttat atcggattat cgaaagtatc acgcagcttt cacaattaac agagaacatc
     7621 ttaaaattga acaaactgga aaaccagcgt atccaactgg aaaagaaaga gtatcgatta
     7681 gatgaacaaa tcagagaagt aatcgttttt tttcagccta aatgggagaa agaacagcta
     7741 gaattggata tcgaacttgc tgcagtaaat tacacaggca atgaagagtt tttgtaccaa
     7801 gtgtggctga atatcatgga taacgccata aagtacaatc aaatcaatgg acagattcat
     7861 atcaaactat ttgagacagc cacagagatt gtactggaag tgaccgattc aggtgtagga
     7921 atgaatgaag agaccagaga tcgaatgttt gaaaaatttt atcaaggaga tacaagtcgt
     7981 caaatttctg ggaatgggct aggactttca ctggttaaaa aaattctaga actgcatgat
     8041 ggacgtattg attatagcag tatcgaagga gtaggaacga cagcaatgat acggttaagt
     8101 aaacaatagg aaaaagaata aaacccctaa aaattctatt gaaggttttt aggggttttt
     8161 agtcgttttt ttgtgattag ggaaatgttc ttacggttct tttggcataa aaatcgctgc
     8221 tttcttattg aactacgaga aatgttagag gtgtcattga aacaaggaca tgatgcaaga
     8281 aagagagcga aagtcatgcg tcgctctctt tttttgggtg gatagatgga cagaaaatgt
     8341 agggcttagt tttcttcttg atttaaatac tcccaaaatg tatctgaagc caaagaatgc
     8401 tcaccgactt tacgagtaaa tgcaagataa ctctctgttg ttggagaaat agggagaatc
     8461 acagtatttt cttttaatag attcttgact gttttttcca taacaatagc aatccccatg
     8521 tcatttgcaa tcatattgat gatcaagtcg attcttgctc ctttataaga gatttttggt
     8581 tcaaatccag cttcgcgaca cagatctagt acaggttgat acaagttcgt agtggggcca
     8641 aggagcaaaa agttctcctt tttcagttcg cttagatcca atttcttttt actagctaaa
     8701 ggatgatttt taggtagaac tgctacaaaa tcgtctgttt cagtaggcaa ccactcaatc
     8761 gaacttggtg gggcctggaa acttctagca aagtggatcg tgtcactatg ttcattggcg
     8821 tcattgtaag ttaattcgac actttccact tctttcaact ggactgtaaa ttcaggatgg
     8881 cgtttcaaaa actcggtgat tttggaaaaa cctttataat tgatcattgt cggtatcgtt
     8941 aagatattta atgaaagatt gttgtctttc gtataggttt caagcacttc ttgcatagca
     9001 tggtattgtt ctacgacatt ttttacatat gggagcaata agcttccagc ttcggtcaat
     9061 tcgatttttc tatgggcgcg ttcgaataat ttgacgtcta attctgtttc taacgaaaga
     9121 atctgtttag aaatatttcc ttgggtggtg aattgtcgat cagcagtttt ggtgaaattc
     9181 attgtttcgg ctaaatcaag aaaaacttcg aatttatgga tatccatcat attcctccta
     9241 cttattccaa taaagaatac tttaacacaa aaaatgaatt ggtgtaatct tctctgtctg
     9301 ttacaatctt tcttgcagcg aaagcaataa ttacagaaat ttttctgatt tttttcgctt
     9361 cgtagaaaaa gagaggagtt ttttgatgcc aactggagtt ttgatcaatg taggttccgt
     9421 tttgtttggc ggactaattg gcggattagt aggaaataaa ttaagtgaac attttaaagc
     9481 acagctgacg atggtttttg gtgtgtgttc gatggggatg ggaatctatt ccattgcgcc
     9541 gatgaaaaat atgccggctg ttatttttgc tttagtgata ggaacagcga ttggtttgat
     9601 tgttcattta ggtaatggga taaataaggg ggctgcgctg atgcaagtac ctatttctaa
     9661 aatcttccct agtgaaaagc ttggtatgac ccacgatgaa tttatcagca cattagtgac
     9721 ggtcattgtt ctattttgtg caagtggtac ggggatctat ggaagtttag attctgggat
     9781 gacaggagac agtacgattt tgatttctaa atctgtcctt gattttttca cagcagctat
     9841 ttttgcgtgt aatttagggt atgtcgtttc tgtgatagca attccccaat tcattatttt
     9901 ttatattttg ttcttattgg caaaatttat ttatccattg actacaccag acatgatctt
     9961 agattttaaa gcttgcggcg gattcttgat ggtcgctacc ggttttagaa tgatcaacgt
    10021 gaaaatgttc ccagtagctg acatgatacc tgcaatgatc gttatcatgc cactgagctg
    10081 gatgtggaca aattggatta tgccactgtt ataataaaaa tcaaagagaa gccatttttt
    10141 gctgactaaa aaggatcaaa tcttttagtc agtttttttg tatttgacaa actgattttg
    10201 aaactatatt atatgttgta taatatagat tgaaataaaa tattacactt gagaatagaa
    10261 ggtgacgtaa tgcacatcca gattccaact gttcttcttg atggtactgt gttagcagca
    10321 ttaagcaagg aagatatgta tgggtatgcc ttaacaaaaa tcgtacaaga aagtctctct
    10381 gtcagtgaat ctacactata tccggttttg agaaggttga aaaaagaagg atatcttgag
    10441 acctatgatg aaccttttga aggcagaaac cgtcgatatt actcgttgac accaatggga
    10501 agtcgtcatt tagaagaaat tattgatgaa tggattgttt ttcgagattc tttagaccgg
    10561 ttattggagg agagcacaaa tgaatgaata tttgaatgaa gttgtggatc atcttcaaga
    10621 aattcctgaa gacgatcgtt ttgatttgat ccagtattat gaggaatatt ttttagattc
    10681 tggaaaaaca ttagatcaga tcattgatga atatggtaca ccaaagcagt ttgccttaaa
    10741 attaaaaatc agttactttt cagaagaaga acaagcagaa aacgagttag tgaaaaatcc
    10801 aaaaagacaa attcgcctga tttggttaat cattatcggt ttatttgctt ctcctgtcct
    10861 cattccacta gcactggggt ttatattggt tattggggga ctattggttg ctttactagc
    10921 gctcattttt agtatctatg ttttatgtat cagtatctta ggtgttggcc tattcatgat
    10981 catctcagga atcagtgtgt ttggacaatc gattctgagt ggattattct ttatcggtgt
    11041 cggtctgttc gccacaggaa gcgcagtatt tatttcgcca ctgttgctga aaggaacaaa
    11101 agggcttttt tatgtcatga tgaactttgt taaatggatg ggacgacgtt ttattacaaa
    11161 aagagatctg cattcagcgg gcgtttgaag gaggacacat aaattgagat tgaaacattt
    11221 tttgattacc ggtattggtc tgatgcttac tggtggcctc ctagcaggtg caacttatac
    11281 attaggcgct caaaagagtc tgacttggga taatggtcct cggttgatcg atatggttac
    11341 tgagacaaaa aaaatcaagg atgcaaacaa aatcgtcatc catgcagaaa atcaaacagt
    11401 ttcggtcata cgcggattgg attttgagat aaaaacgact tatgataaga cagaaaagcc
    11461 agaaataaag gagaaaaatg gcgtgttgac tgtgaatgca gtcaaaaaat caacaggtgc
    11521 aatcattagt ttagaagaca caagtgcaca aatcactatt gttgtccctg cagatcatga
    11581 attatctgaa ttgacaatcg aagggaataa tagtgcaatt gatatcaacg aagtatcaat
    11641 caaaaaagtg aatactaaga cacatgatgg ctgggtatcg ttcaatgaat ttacaggagg
    11701 aacaataact gcgaaagatg aattaggctc acttcgacta gatgaggcca atgtggataa
    11761 cttgatcgtc acaggtgaaa acagcaactt ccatctagaa gacagtttga tcagcaaaga
    11821 aggaaaagtc atcctaaacg gtggtaattt atctttgcat gagatggaaa acagtggcta
    11881 tactatccaa acgtctggtg aatcttatat tgaaaaagac taccagccaa taacaaatac
    11941 tttgttagaa caaggacaga aaaagctaaa gatcactgct aaagacacat cgatcaatat
    12001 tacgacggat caagaggaca atgaaacaga ttgatcctgt actcaactgg atattttaag
    12061 aaaaaacagc aaagatcctt ccaatctttg ctgtttttca tcgcttaacg cacataacta
    12121 tagtaaaata agaaagaaaa tatctaaaaa gaaaacaggg tgaaagagta tatgaaggta
    12181 gaagaaggtc aaagagaaaa gttaaagaca gaaatggaaa gattacatac ttatatcact
    12241 caactaagcc aaaccttcta tgatccagac aaagaaaaag tgatggttaa ttacccgaac
    12301 aattcagaag gtcgccaatt agagcaagtg taccatgaag ttttcaaaca tttattgacg
    12361 gtgaagaaag agctggatta ttattcttta ccgattatcg atacaggaat attgaagtat
    12421 gatgggaaga aggaacgatt tatctttaaa tctgttcggg aagatttgcc tctttcagca
    12481 gggatggatc tagaggtctt ggtagaagac tattttacag aagaaaagca ctgggttcga
    12541 acaaaattgg attatttacc gcaggcagcc ggagggacac aggcaagcgg ttggtacatc
    12601 actgaagaca aagagttaga actagaaggt gtaatggcgc gcattcgaaa aaaaattaaa
    12661 aaagtaacat tagatattta ttgaaggata aaggagcgca ggaagtagca tgagagaaaa
    12721 aagagcattt tatatcaatg gttatgcagg gattgtcgga ttggtcgtat tagctttagt
    12781 tggattgttt ttcttctatt taggcatgtg gcaagcgaaa gtttgggcac tttttttaag
    12841 tatttttctt tggttgatca cgttattgtt gttaagttcg gcaactgtag tcagtcctaa
    12901 tcaagcaaaa gtaattttgt tttttggaca atatttaggt acgattcgag aaaatggctt
    12961 tttccttacg atacctttgg cacaaaaaat gactgtttcg ttgaaagttc ggaatttcaa
    13021 tagttctgtt ttaaaagtca atgatttgga tggaaacccc atcgaaatct cagcagtagt
    13081 agtctttaaa gtcgttgata cagcaaaggc attattcgat gtagcgtatt atcaagattt
    13141 tgtagaaatc caaagtgaga cagccattcg gcatatcgct agccaatacc cgtatgatac
    13201 attcaatgag aatgatttga cgcttcgagg aaacacgacg gcagtgtctg acgagttgca
    13261 aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa gtaatcgaaa cacgcttgaa
    13321 ccatttagcc tatgcaacag aaatcgctag tgccatgctc caacgtcaac aagcaaaagc
    13381 cattttgtct gctcgacaaa ccattgttga aggagctgtc acaatcactc aaatggcctt
    13441 agagcaaatc gaagatggac aagaaatcaa tttcaccgat gatcggaaag tccaattgat
    13501 caataattta cttgtctcaa tcattacaga caaaggaacg caacctgtta tcaatacagg
    13561 cgacatgaaa gaccgtaagt gacatacttt ttgtaagata ggaagaagga gtttaggacg
    13621 gattattgtc ttgaactctt tttttagttg ttttaaagaa aatgacaaaa ttgaaagaag
    13681 aatgatagtt gattcgaagg cagaattgtt cgcttttttc tataatcagt atataaagta
    13741 agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct acttgttttc atcgttgtgg
    13801 tacttgcagg ggtgaaaatc gcagattacg tagttatggg tggagaagaa tactatgtac
    13861 aaattacgac agatggtaca aaaacagttt caaaatcaga ctctggacaa gaaacagtca
    13921 actatagata tatcctgcct ggctatacga aagagggtaa ggaaaaagaa ctggacttca
    13981 agggacaatt gactcgaccg cttcgtaaag gagcatattt aaaaatcacc tggaacaaaa
    14041 ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga cgttccgaaa acagcactgg
    14101 aaaaactaat taaagggtag ggaaagaaat gaacaaaata gtcgaagtac agaatgtaac
    14161 aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca ttaagcggca tcagcttcga
    14221 tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct ggttctggta aatcgacact
    14281 tttgaatatt ttatccacat tagataaacc aacagatggg catatccgaa tcaatcaaaa
    14341 agatgtgacc acattaaaag gaaatcaact agcagatttt cgtgcgaatg agattggctt
    14401 tattttccaa gacttcaatt tattagaaag cttgacagca caagaaaata tcgctgttcc
    14461 tctttcttta caaggcgtca aaccaaaaga aatcaagcaa cgtgtccaaa aaattgcaga
    14521 acgtttatcc atctctcata ttttggaaag ctatccttct gagatttctg gtggacaaaa
    14581 acaacgggtc gccgccgcac gtgcattgat cacacaacca acgattctat taggggatga
    14641 gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt ttagatacga tggacgaatt
    14701 gaatacaaaa gatcatgtat cgattcttct tgtgacgcat gatccgtttt ctgcaagtta
    14761 ttgccaacgt attttattca tcaaggatgg cggtatccat caagaagtga aacgtggaga
    14821 tcaaagccgt gattccttct atcgagaaat actatctatt cttggaaact tggaacagta
    14881 gggggagatt gactatgctt tggaaattat cacttactgg gatcaagagc cgtttaagag
    14941 actacctggt gttattctca ggattagtta tggcatctgc tattttttac atgtttgaat
    15001 caatggcaag caacgaagct tttttgaaaa gtaatacgat cgttgcggct acagttatca
    15061 ttttccgatt cggttctgtt ttactaggga tcatcacatt cgtttatatt ttatatgcaa
    15121 attccttttt gatgacgatg cggcaaaaag actatgcaat gtttatgatg ctaggagcaa
    15181 aaggcagaaa aatcgcacag atgatttttt ctgaaacatt cttagtaggg atcgcagcta
    15241 ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat cgtccaccga ttattagtgg
    15301 atcagttaga tctcaatatt tcgcatttta caccttttag tattcacggg ttgttgatca
    15361 ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt tgtcaatgct ttttcgattg
    15421 tcaaaaaacc gatattagca ttgattcgtg cggatcagac accaacaaga atcaagcagc
    15481 ataaattctt gtttttcctt gaagtagtac tgggaattgc ttttctaggc tgcggttact
    15541 atatgatgaa acacgtgggg acctatcaag tctttggtat aggagtagcc ttagttacga
    15601 tcgtattagg aacttatttc attttccatt ctgtgatcat tttcttttta tcattactaa
    15661 agaaatcaga acagatttca ttgaaaaaat tgaacaattt cacattgtca cagttaaact
    15721 ttcgtattcg tgaatataca caaatgctct caatggtcgc tatgctgttt gccttggcac
    15781 tgggcgcttt gacggtagga ttaggtttta aaaatgaaat tgcagacttg acagaaaaca
    15841 attctggata tgatctagtg atgaataatc ctcgggcaga agatcagcaa aaaatcaaag
    15901 aactttcacc aacactttct gcttcttata ctcaaaaaga agatgcggga acgatttact
    15961 ataatgcaag tgaatttgcg gctcaaccat tgatcatgat aaaacatgaa gatggaacac
    16021 cgccgaagat cacgaaagaa aaagttcctg ctgaaaaaat gaatgaactg actgtacaag
    16081 aggaacttcg aagctatgaa ttgccggaac agcaagaaaa agaaatcaag gtagtcagtc
    16141 aatcagaatt tgatcaactg aacttgccac aatccacttt acagctagtt caggtaaaag
    16201 acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc tcaaaataaa atgaacaatc
    16261 catctttgca aaaagtagag tacagcaacc agaaatatga aatgtatgaa gtgtataatg
    16321 gtctattctc tggttttgaa tttatgggat tcttcctagg tttagctttc ttgaccatgc
    16381 ttgctagctg tttgatgttc aagatcttgt caggaagcaa gagtgacatt gctcgttatg
    16441 aaatgcttga aaagatagga acacgacgcg ggttgcttaa acaatcgatc cgtagagaag
    16501 taggcgtgtt attcttagca ccaggaatct taggtgtagt ccatgtctta ttcggattac
    16561 aaatgtttgg tctattcatg caaaatccat acaaagatat ttgggttccg tttacgatct
    16621 tcttcgtatt gtatttcatc tactatgtac tgacgacatg gttatatact ggaatcgtat
    16681 tgaaaaagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa
    16741 tagctcctcc tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
    16801 cgtttgttcg gaaaaagagg ttgtgacaat atttttgtca caaccttttt ttcacggtct
    16861 tttattagaa tatagtaaat tttttctcgt tactcacgga ataagctgtt aagaaaacgg
    16921 tttccatgta tactggaatc agtgcaaatg aaagaaaaga gggtgcaagg aacatggaaa
    16981 tcaatgaaca aaattttggc gaaaactatc acttgattac gatcacgaat caaaatggga
    17041 catctttgtc tgtcagtgat cttggggcac gggttgttca ttttcaaaca ataatcgata
    17101 atgaaaaacg agagttgatc ttaggttttg atgaagcaaa agaatatcta gaaaaagacc
    17161 cttatatcgg tgcttcaatc ggacgtatgg ctggacggat tacgaatggc acattcaaat
    17221 tagatgatga aacgttcgaa gtaaaaacag acccggacac tggtcattgt ttacatggtg
    17281 gtgcacctag ctttgaagcc aagcagtggc attatacagt catcaatggt gaaaacgaag
    17341 catcggtcat tttctatcag actagcccag acatggaaaa cggatttcct ggaactttag
    17401 atgtagaagt acggtatacg ctgacgaacg atgatacatg gcgagtgacg gtccaaggga
    17461 tcagtgaccg caaaacagta ttcaatccta caaatcatgt ctatttcaat ttaaccggtg
    17521 atccagcaca gtcaatcgac gaacatatac tttggttgaa tagccgatta tttgctacgc
    17581 tcaataaaga tacaacgcct acaggagaag tagtttcagt aagtggcaca gcatttgact
    17641 tccaacgtcc taaaaaatta ggtgaggtct ttgccagtga ttttcgccaa tcaatgatag
    17701 tgggcggtat cgatcatcca tttttgttga aaaaacaatc tggtttacta aatgctgcgg
    17761 aattgacaag cccagatgaa aaagtatcga tccaagtaaa aacagatgct tcaggaatcg
    17821 tgatttttac agcaaatttt ggagaagatg gaccggaaat gcgcgggaat aaattagtaa
    17881 atcatggcgg tatcacattt gagactcaag aactgccagg agcggagcga cttgaaagtt
    17941 ttggagatgt cactctggaa cctaaccaag tacgcgagtt tgtaacgg
//
LOCUS       DAHEYX010000039        17714 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHEYX010000039 DAHEYX010000000
VERSION     DAHEYX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.039"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            3..815
                     /locus_tag="MB219_000915"
     CDS             3..815
                     /locus_tag="MB219_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCC8426648.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="MB219_000916"
     CDS             812..1612
                     /locus_tag="MB219_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCC8426649.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="MB219_000917"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="MB219_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCC8426650.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="MB219_000918"
     CDS             3109..4818
                     /locus_tag="MB219_000918"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCC8426651.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="MB219_000919"
     CDS             5030..9382
                     /locus_tag="MB219_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCC8426652.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="MB219_000920"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="MB219_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCC8426653.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="MB219_000921"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="MB219_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCC8426654.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="MB219_000922"
     CDS             11220..11513
                     /locus_tag="MB219_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCC8426655.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="MB219_000923"
     CDS             11510..11821
                     /locus_tag="MB219_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCC8426656.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="MB219_000924"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="MB219_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCC8426657.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="MB219_000925"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="MB219_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCC8426658.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="MB219_000926"
     CDS             complement(14622..14843)
                     /locus_tag="MB219_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8426659.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17654
                     /locus_tag="MB219_000927"
     CDS             15291..17654
                     /locus_tag="MB219_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCC8426660.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWELFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6035 a         3146 c         3903 g         4630 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgct ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaatccaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatatcgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caagcgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggag cttttcttta ttgaaggaaa tgatccagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat caaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaattgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatacgga
    16801 aatcacatga cctttcttgg cgaaaagatt atccttcgct gaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcgg aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaaca gaatttttta cattgtatta
    17701 tttgtcaaat taag
//
LOCUS       DAHEYX010000040        17358 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHEYX010000040 DAHEYX010000000
VERSION     DAHEYX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.040"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            203..1969
                     /locus_tag="MB219_000928"
     CDS             203..1969
                     /locus_tag="MB219_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCC8426661.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2071..3330
                     /locus_tag="MB219_000929"
     CDS             2071..3330
                     /locus_tag="MB219_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCC8426662.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3477..4778
                     /locus_tag="MB219_000930"
     CDS             3477..4778
                     /locus_tag="MB219_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8426663.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4781..5632
                     /locus_tag="MB219_000931"
     CDS             4781..5632
                     /locus_tag="MB219_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8426664.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5645..6460
                     /locus_tag="MB219_000932"
     CDS             5645..6460
                     /locus_tag="MB219_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCC8426665.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6611..7777
                     /locus_tag="MB219_000933"
     CDS             6611..7777
                     /locus_tag="MB219_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCC8426666.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8007..8654
                     /locus_tag="MB219_000934"
     CDS             8007..8654
                     /locus_tag="MB219_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCC8426667.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8738..9682
                     /locus_tag="MB219_000935"
     CDS             8738..9682
                     /locus_tag="MB219_000935"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCC8426668.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9817..11268
                     /locus_tag="MB219_000936"
     CDS             9817..11268
                     /locus_tag="MB219_000936"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCC8426669.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11289..12125
                     /locus_tag="MB219_000937"
     CDS             11289..12125
                     /locus_tag="MB219_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCC8426670.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12125..12784
                     /locus_tag="MB219_000938"
     CDS             12125..12784
                     /locus_tag="MB219_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCC8426671.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12910..13599
                     /locus_tag="MB219_000939"
     CDS             12910..13599
                     /locus_tag="MB219_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426672.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13605..14561
                     /locus_tag="MB219_000940"
     CDS             13605..14561
                     /locus_tag="MB219_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCC8426673.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14605..15117
                     /locus_tag="MB219_000941"
     CDS             14605..15117
                     /locus_tag="MB219_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8426674.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15373..15921)
                     /locus_tag="MB219_000942"
     CDS             complement(15373..15921)
                     /locus_tag="MB219_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8426675.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16010..17191)
                     /locus_tag="MB219_000943"
     CDS             complement(16010..17191)
                     /locus_tag="MB219_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCC8426676.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5715 a         3088 c         3473 g         5082 t
ORIGIN      
        1 gcttaagata aattcaggac actaaatagt acaggaagta gactaaaact tttaaaagtt
       61 ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc
      121 gataacattt ttgaaagcgt tgtcttttgc ttctctatca cttatagtga tgacgtcata
      181 ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac cggaaagtga
      241 atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta ggaaaaatga
      301 tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc ataacaactg aaaaatggta
      361 tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt attgggaaat
      421 tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc catgttgttg gggaagataa
      481 cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg tgattactga
      541 accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca ctattccaga
      601 atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa acggggacaa
      661 agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc gagaagactt
      721 tttcggggga gatttgcaag gtgtgatgga tcatttggat tacttgacgg accttggcat
      781 caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat acgatacgat
      841 tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa acttgattga
      901 tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc atatgggtgt
      961 ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct ataaagactg
     1021 gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac cagaaactgc
     1081 tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac taaatacagc
     1141 aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa aagaattcga
     1201 tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt ggaaaaaatt
     1261 cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga tttggcattc
     1321 ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg catttacaga
     1381 ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg tcagtggaat
     1441 gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca acttattaga
     1501 ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc
     1561 tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg ggacagaaat
     1621 agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg agaaggagga
     1681 acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga aacagttgtc
     1741 taagattatt tctgaaggtt ctactcagtg gctaatagta aatgatcgtg aagataagct
     1801 ctatttcaca cggaaactag agggacagat catctatgtt tactttaatc agtcaaaaga
     1861 accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc agttgttaga
     1921 agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa aactgttacc
     1981 ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata agggtgtaac
     2041 agcacgttga atacaaagga ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt
     2101 ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa tggtaactct
     2161 tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga ttctggatat
     2221 aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag
     2281 ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg gcctgctgga
     2341 aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg acagcaagga
     2401 catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga caaagcccaa
     2461 gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct tgttttgtac
     2521 tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga gaatctatcc
     2581 aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa
     2641 tggactgact tttattattc ctatggttta attgcaggtt acggtggcta tgtatttggt
     2701 gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt agaaggaatc
     2761 tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt
     2821 gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat cattgacggt
     2881 ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc caaaattcct
     2941 actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc
     3001 aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac gaatcaggaa
     3061 aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca agctagggaa
     3121 acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata tgaaacagct
     3181 caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga aaacttgatg
     3241 ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc agtaaaaaca
     3301 atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat ttttataaaa
     3361 aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta tcttgctctt
     3421 gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc attcagatgc
     3481 agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca cttattccag
     3541 ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc gccatattcg
     3601 tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga ttggttactt
     3661 tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc actttgcaat
     3721 tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac gcttatcgag
     3781 tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata
     3841 atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt ctcatgattt
     3901 ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat tatgacttca
     3961 atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt tttagtatct
     4021 tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc gtgatttgga
     4081 ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt gttgctcacc
     4141 agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca tgggcggtcc
     4201 cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta ggtgccatca
     4261 atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt gcaatctctt
     4321 ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc tggttaggat
     4381 ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa gacttatatg
     4441 aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg
     4501 ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat ttcaataatt
     4561 tctccatgat ctatctgttt aacaatggcg gtcccggaag cgtaggcgga ggagctggtt
     4621 ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct ccgcagtatt
     4681 ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg
     4741 tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat ttcattcgag
     4801 tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt taatactgtc
     4861 agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt caggaaatat
     4921 tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta ctcggctatt
     4981 cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg tcctaacaat
     5041 gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct atcgttttat
     5101 cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga ctatggcagc
     5161 attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt actggttctt
     5221 gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga aaggatactt
     5281 tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag ggcatttcag
     5341 gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc aagcattatg
     5401 ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc gaacacctga
     5461 aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa accaaagagt
     5521 ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat tattcttctt
     5581 tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat aggagcagag
     5641 tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa ctcaagtgtt
     5701 tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat ttttagtttc
     5761 cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga ttccattaga
     5821 acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat tttctgaact
     5881 tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga cccctgaaat
     5941 tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt ttattgattt
     6001 tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc gctatacgat
     6061 gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt tccaacgatt
     6121 tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt atagtttaag
     6181 tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt cctttttctt
     6241 gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga gtgcaaattt
     6301 gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt ttatccactt
     6361 tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc ttttatgggt
     6421 ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag cgatttatgc
     6481 gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt
     6541 aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag tttttttaag
     6601 gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt ctttctcgct
     6661 ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata cggacactcc
     6721 tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta caagtaacgc
     6781 ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg gaagtatcca
     6841 agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa aagattatct
     6901 aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag atatccatgc
     6961 gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt tgacttattc
     7021 ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca ataaagtcca
     7081 tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg
     7141 ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa ctgggatcac
     7201 actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac atttagaaga
     7261 taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat
     7321 cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt
     7381 ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca gccgacaaca
     7441 aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt atgtccctgg
     7501 tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg atttggaaag
     7561 cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc atcaagtcca
     7621 agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc tatatttaca
     7681 agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt tgcttgttca
     7741 acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt actgtttaaa
     7801 gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt ttgtctaact
     7861 tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat aaaagtttgg
     7921 ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta atagaatact
     7981 attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact agtttatttg
     8041 cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct gggaatacgt
     8101 cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa gcaagcacat
     8161 cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat atcaaagtgt
     8221 ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca attactagtt
     8281 tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc gatgatcaaa
     8341 aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag aatccagaaa
     8401 ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg gatgttgata
     8461 aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg
     8521 caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc aaagtaaaag
     8581 acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta ctgtcaactg
     8641 aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt agtcatgata
     8701 cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa cgacctttgc
     8761 caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca tgactaccca
     8821 ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga gctgtacgat
     8881 caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag ggagtatcaa
     8941 ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc ttgcgtatga
     9001 aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga tgagtgtcca
     9061 ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga tcactgaatt
     9121 aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt ttgaggccac
     9181 ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa tcaaattgcc
     9241 tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc agtttccatt
     9301 gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata cagaacaaga
     9361 agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt atatcaagcc
     9421 gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa tccaaatcat
     9481 tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat gtggcgcaag
     9541 catgttacag attggtaccg agcttcataa agaagggccg gaaatttttt ctagaatcat
     9601 aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg aatttaaagg
     9661 caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt cgtctttttt
     9721 tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg ttagacgaac
     9781 ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac agaattagca
     9841 aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat acagaaaaaa
     9901 ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat
     9961 tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat cccatttcct
    10021 ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc tcgtttattt
    10081 gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc gataggtcta
    10141 gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac agacccagtc
    10201 atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg atcaagtgga
    10261 ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag tgatggcggt
    10321 ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg
    10381 acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat tgatttcggc
    10441 ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag tatccattcc
    10501 ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa aaaacggttg
    10561 aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga ggcagtactt
    10621 gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac agagattcct
    10681 tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg cgcagaaaca
    10741 gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa agaagaactt
    10801 gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc tacttacatc
    10861 gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca tcaagaatac
    10921 gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga agatttgcaa
    10981 agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt agaaggttta
    11041 gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac acagttggct
    11101 aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga aaacatgcct
    11161 ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca aatcggctat
    11221 ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca gaaacaagga
    11281 gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt tttattttaa
    11341 gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat gtagaaaaaa
    11401 agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat tataatgatc
    11461 aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg
    11521 tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt tcaaaaagag
    11581 aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa aaattctccc
    11641 tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag
    11701 gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc aatactagct
    11761 atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat gccacgaatg
    11821 atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat gattattatc
    11881 tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg gagaacgttt
    11941 atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga
    12001 aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa atatctgaaa
    12061 aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg
    12121 agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc ctttcgtact
    12181 gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag gaaatctggt
    12241 agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg tatttattcg
    12301 tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc tgttatattt
    12361 tgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta cgattaccag
    12421 cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt caggtcagtg
    12481 ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata tcgttttgcc
    12541 gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc tagtgaaagg
    12601 tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa agatcatagg
    12661 cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt attgggtcat
    12721 ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatgc atcagtcaat
    12781 ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg tttcttgtat
    12841 attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat atgttcggag
    12901 gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata ccaatattgc
    12961 atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca aggaattgta
    13021 gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg ctatgagctg
    13081 aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg acattataat
    13141 tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc tacaggaata
    13201 ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc tggtacattt
    13261 ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc tttgacaaac
    13321 cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga gcgaggcgta
    13381 aaatattttc caacagatca attgtacaaa gaattgaaaa gacgttcaga aaatcaagat
    13441 ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag tcaggcaatc
    13501 aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt gaggcaacaa
    13561 cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac tatccttatt
    13621 taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga ggattgctct
    13681 ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt aaaaatgtga
    13741 tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc caagtaggac
    13801 aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat agctctgtcg
    13861 tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca cctccagcgt
    13921 ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa cagttggcag
    13981 gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa
    14041 acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt tatgcagata
    14101 aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga tatattggat
    14161 tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct agccgagcgc
    14221 ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa cattttgtct
    14281 ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca ggagataaaa
    14341 tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga atgattgaat
    14401 tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa aagtggtcac
    14461 ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat tggcttaaat
    14521 attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat gtaaatgaaa
    14581 aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga agaagtagaa
    14641 gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa
    14701 tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga aagaaaagaa
    14761 agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt ctcaagcgtg
    14821 gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc ttatttatcg
    14881 attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa accgttctta
    14941 gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat cgatacttat
    15001 ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta tcaagggatg
    15061 attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat caattgacta
    15121 taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa tcatatgata
    15181 gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg
    15241 cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc
    15301 cttcatcgag aggtcgtgac atagcttttg tcacgacctc tcggcttatc ctacgagtat
    15361 tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt cttggaacgc
    15421 tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca actgtgctaa
    15481 atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc cacgtttttt
    15541 caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga ctaccgtccc
    15601 gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac
    15661 tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag catattggtc
    15721 ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag aaacaaaata
    15781 ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag taatgatctt
    15841 ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag aaggaccgat
    15901 aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag tgaaaaagct
    15961 ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtatat tataccagca
    16021 agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc
    16081 cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca ccagaaatga
    16141 tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt gctgttgtac
    16201 gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat tcatttgtaa
    16261 ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg attccgctga
    16321 tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc
    16381 cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc atagcagcaa
    16441 ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg aaaaatgtct
    16501 gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca tagggattta
    16561 caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg atcacatatt
    16621 tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta gacatagccg
    16681 atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt tttatggaaa
    16741 taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat tccaatttgc
    16801 ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat ggaagaatct
    16861 tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc aatacggaaa
    16921 gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca acaccttgcc
    16981 cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc gctgtaccaa
    17041 aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc acaattagtg
    17101 gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa
    17161 ataaaccaat aatacctaat aaataactca aataatcact cctattccaa attccgtttt
    17221 attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt acttttttag
    17281 agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag gtttaaaaag
    17341 tgtaacgtct tagtaaag
//
LOCUS       DAHEYX010000041        17345 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHEYX010000041 DAHEYX010000000
VERSION     DAHEYX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.041"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            46..858
                     /locus_tag="MB219_000944"
                     /pseudo
     CDS             46..858
                     /locus_tag="MB219_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            871..1599
                     /locus_tag="MB219_000945"
     CDS             871..1599
                     /locus_tag="MB219_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426677.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1668..3194)
                     /locus_tag="MB219_000946"
     CDS             complement(1668..3194)
                     /locus_tag="MB219_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCC8426678.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            3446..4627
                     /locus_tag="MB219_000947"
     CDS             3446..4627
                     /locus_tag="MB219_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCC8426679.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4687..6315)
                     /gene="treC"
                     /locus_tag="MB219_000948"
     CDS             complement(4687..6315)
                     /gene="treC"
                     /locus_tag="MB219_000948"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCC8426680.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(6712..9423)
                     /locus_tag="MB219_000949"
     CDS             complement(6712..9423)
                     /locus_tag="MB219_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426681.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9433..10134)
                     /locus_tag="MB219_000950"
     CDS             complement(9433..10134)
                     /locus_tag="MB219_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426682.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10319..10921
                     /locus_tag="MB219_000951"
     CDS             10319..10921
                     /locus_tag="MB219_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCC8426683.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11002..12015
                     /locus_tag="MB219_000952"
     CDS             11002..12015
                     /locus_tag="MB219_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCC8426684.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12124..13041
                     /gene="rbsK"
                     /locus_tag="MB219_000953"
     CDS             12124..13041
                     /gene="rbsK"
                     /locus_tag="MB219_000953"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCC8426685.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13069..13464
                     /gene="rbsD"
                     /locus_tag="MB219_000954"
     CDS             13069..13464
                     /gene="rbsD"
                     /locus_tag="MB219_000954"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCC8426686.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13464..14837
                     /locus_tag="MB219_000955"
     CDS             13464..14837
                     /locus_tag="MB219_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8426687.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            15194..17338
                     /locus_tag="MB219_000956"
     CDS             15194..17338
                     /locus_tag="MB219_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCC8426688.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         5198 a         3434 c         3193 g         5520 t
ORIGIN      
        1 ctttgagata ttctttagga tattaaaatt aactagcacc acgggtacta tatcattatt
       61 ttttataccc gtttttctgg tgaaacacct gatgtcttag ctttgaatac gagttttttc
      121 attctatatc tgtgtgttgg cctgatcatc ttggtttatt attggaaaat ctctaataag
      181 aaattagctg atcagttatt agagcaacaa gtcaggatgc aacaggatta tatcaaagat
      241 ctagaaaaaa actatcaaga cttgagagaa ttcaaacatg attaccaaaa tctgcttttt
      301 tctcttcaca gctatatttc agaaggcgac ttagaaggat tacggtctta ttatgctcaa
      361 acgatattac ctacacgcca gatgatgaac ctttttccgg ccaatctaag tatgttggat
      421 aatatgaaag ttcctgagat ccgtagttta ctgtcattaa aactcatgat ggcacaggaa
      481 aaagggttgg tcgtccagct tcattttcct gagccaatcg aactggatac gaaacatacg
      541 gtaaaccttg tacgtatgct tggtattatc ttggacaatg cgattgaagg agctagtgct
      601 gcaaagaaga aaaaaatcga attgaccata ctcaagaaga atcattccgt gatgattcgt
      661 gtgatcaata caacaacaaa tacattgccg ttaaaccagt tgaaacaaaa agggttctca
      721 acaaaaacaa atccaaaaaa cgaaggcctt ggcttgttta tcttggatga attgacaaag
      781 accaatcctt atcttttcct tgaaacatca attgaaaacc agcgtttttc tcaaacgatt
      841 tatatacaaa taaaataaag gagtaatgat atgattccca tttatatttg tgaagacgat
      901 ccaaaacaat tagaagtttt atcaacggtg atcaaaaata gaatcatgat tgaaaactta
      961 gacagttatg tccatatttc tacctctgat ccggaagaac ttctcaatgc agccaagatc
     1021 agaacttctt cttttggtat ttacttttta gatatcgaac tagaagacag tgaaatggat
     1081 ggtgtagcga tcggtaaatt gatccgtgaa ttagatccct taggaaaaat aatctatgtg
     1141 acttcccata cggaaatgtc gatgaaaatc ttaaaaagca acatcgaacc gactgattat
     1201 atcgtcaaag aagatcttta tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt
     1261 ttcgaagatt ttcaaatgtc ccagatccaa aaagatatct tcaaaataga gtttaatgat
     1321 gagatcaaat ttttaccgat caaagacatc caatattttt ctacttcgct aggagcacca
     1381 cataaactgg aagtccattt gactcatgcc caattacaat tctatgagaa gatcaaagaa
     1441 atcgagaaaa tgcatcggaa ttttatccgt tgtcataaat cctatgttat caatacacaa
     1501 aatgtgcggt cgattaataa aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc
     1561 ccagcctcta tccgtggatt aaagaaattg atttcataaa aagaaccgaa tagtttggac
     1621 cgactaaatg actagccgat ccaaaccctt cggttctttt ttgcctatca gtgcaaaagc
     1681 atttcctgtg caatttcacg tccatccatt gtatctggat aaaatgttgg ccaattctcc
     1741 atttctttca gcaatgcttc ttgactttca tttcctgcat ataaatgaaa atgttcggag
     1801 tcagccggtg caatcgtgtg atcacggaat tgaatatatc taaaagcgcc actttcctta
     1861 tcttctgctt cgaatagata ccttacgcca cggtttcctg cctcgtaggt caagatttct
     1921 ttccctacat aacgataatt tgaacgtttc gttgttccat ccttaaacgt aaatgccatc
     1981 tctttctcac tgatcttgat tgaagcaata tctgtttgat agcccttcgt ataatattct
     2041 ttatattctt gtgctgtctt atcctgattc aatttttctt tatattcaaa aacaggagca
     2101 aaagtcccat cttctacata cgtatctact gtttgccaag ttcctgccca atctgaaaga
     2161 gagcgatctt gtacatcctc gtcttcaaaa taaccgttgt agacgctctt ttcttttttg
     2221 ggggtcaaag tatcttctag cgggttttct gtctctgtcg tttttttcaa cgcttcgagg
     2281 tttgcttcca tgatagaaag atagttttct ccattttcta tttgttcatt cgtcaatcct
     2341 tccaaaggat tcaatacttc taaagataca cctgcttcgt ttgctaacgt tctggcaata
     2401 ctatctttgg cgttttcttc aaaataaata tagttgattc catgttcttt aacaaagtgt
     2461 ttcagttcag caattctcgc cgcagacggt tcttcactag aagaaagacc agcaatcgat
     2521 acttgattca atccatattc taatgccaga tagttgaatg cagcatgttg cgtgacaaaa
     2581 gatctttgtt ttgcttgctt aaaggtttct tgataaagct gatggagtgc ttgaagtttc
     2641 tcggtatagg ctgctgcatt ttttgtccat acttcctgtt tttctgggta agcttcaatc
     2701 agctgatcac gtatgtttgc tacctgcttg attgctaaac ttggagctag ccatacatgt
     2761 gggtctagct catgtgtatg gccttcttcc ccgtgttcat ggtcatgatc atgtccttct
     2821 tcacttcccg gaagtaaaag cataccttct gtcgctttta tcgtatggac attgccttct
     2881 tgtaatgttt tttggattgc ggggacccat gtttccatgt tttcatcatt ataaacaaag
     2941 atatctgtat cttggatctt tgccatgtct ttagcagaag gttcataatc gtgggaatca
     3001 gtccctgctg gaatcaataa ctctacttct ccttcatccc cgacgattgc ttttgtaaaa
     3061 tcatacatag gataaaaggt cgtcatcacg ctcaattttt ctttactgtc ctcggctttc
     3121 ccacttgccc cgccgcaagc agttaacata cctgctaaaa ccagcatggc caatatacct
     3181 atcaatcgtt tcatttatga cactcctctt ctcatcttct ttcatttgaa tgttccgatt
     3241 cgccttttac aaaacgtaat agttacgttt tagcaacagg atttaattta tcatatggtt
     3301 aaaagaactg tcaagcgttt tcttatttta attgatgaga aagtttatat gaatgattga
     3361 cgatattttt gcaatcaacc tctatcattt tccttttata cccgcttttg tctataatga
     3421 agttatagat aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga atcgtttgat
     3481 ccgcttttta ggaggaaaaa cctcttacta tgttttagga atagttatct tacttgcgct
     3541 gatcatttat tttttccatc agatctcgtt tatattcaag cctgtcggaa tcattatcgc
     3601 tactatcttg cccccattat tgtttgcact gatcttgtat tatcttttgg aaccagtcat
     3661 caaacgtttg agcaagcacc tttcgaggaa cttgtcagtc attatcgttt atgtgattat
     3721 tttattttta ttggggcttg gaagcgtatg gcttgttcct tttcttgaag aacaggcaaa
     3781 gacattgatc agtagtttgc cagatttgtt tcaagatttt caagagagtc tccgacaatt
     3841 tttagagaaa acaccttttg ccgattcatt taatcaattt ttcgcttcta ttgacgacgt
     3901 gacgtctgat atagctggat ttattggaaa ttattgggaa agtggcgcac aacggatcag
     3961 caacattttt tcaactgtta cagctatttt tattacttta tttactggtc caattgtcgc
     4021 tttttttctt ttgagagatc ctcaaaaatt ttatcagtca gttttagcga ttgtcccacc
     4081 agcatttcgt acagatttta aaaatttgac aaagattgct aatcaacaat tgggttcatt
     4141 tctaaagggg caaatcattg cttcatttat ccttggagca gtctactggg tttgtttctt
     4201 actgatcgga ttagaatttg ctagtgtttt agctatttct gctgggttgt tatgtatcat
     4261 tccatatatt gggccgttta tcgccttctt tccaggtttg atcattgcat tccaagactc
     4321 tacttttatg gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc tccacggtga
     4381 cttggtgatt cctcgagtaa tgggtgatcg tttacagatc catccgatta cgatcttaat
     4441 cgttttactt gtcatgggtg atctaatggg aattgtgggc gttatttttg gtattcctat
     4501 ctatacgtta gtcaaattac tcgttacttt tattttccgt aaattcaaac aacgctacaa
     4561 taaattttat ggcgataaag gagagtatga aaactcttca ttttctgaag aagattattt
     4621 taaataaaac acaaaacgtc gatcttacga gaatagctcg taggaacgac gttttgtgtt
     4681 tttgttttaa tttttcagca aagcaattgc ctggtatggt tttagctcca gactttgatg
     4741 aagaacagta tcggaataat tatcgataag aatattggct tctgtgaatc cttctgggat
     4801 ggtgatcctt gtatctttat catagaaatt tgttaataca agcagctgct gtccttcaaa
     4861 ttggcgaaga aagccataaa cttccggatg atttgcttca taagcatgat aatctccttg
     4921 tgcaacgatt ggatatgttt ttctcagccg aatcaatttc ttgtaatagg acaaaataga
     4981 atcttcagaa gatagttctt tttctacatt aatctcttct agcttgcctc caacttttaa
     5041 ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct gaccattgca tgggtgttcg
     5101 agagttatcg cgagattttg ctttgatccg acgaaacgct tctttttctg tgaatccttg
     5161 ttcaaggagt aacttataag cattcaaact ttctacgtca atatagtcgg aaatcgatga
     5221 atagtctggg tcgatcatgc cgatttcttc tcccatataa atatagggcg tcccacggtt
     5281 caaatggatc atggttgcta gcatctctgc tccttgtttt cgatactgat cgatagctac
     5341 aaatcgattg agcgcacgtg gctgatcatg attattaaaa aatagagcat tccagccgtt
     5401 accctcactc atcttttctc cccatgtatg aaatagtcgc tttaattctg caaaatcaaa
     5461 tggaggagta gaccatttat tcccattttc ataatcgact ttcaaatgat ggaaattaaa
     5521 gaccattgat aattcttctc tactaggttc tgtatacaag atacaatttt cgatcgtcgt
     5581 agagctcatt tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg cttggttcaa
     5641 ttcttttaga tacgtatggg tgattggttt gtccgtatat tcaggtttgc catcattttc
     5701 tttgttattt ttcaattgtt cgtctttacc gataacattg ataacatcga aacggaatcc
     5761 ttttacccct ttttccatcc aaaaacgaac aactttgaat agttcctctc ttactgcttg
     5821 gttccgccag ttcaagtcag cttgtgtccg atcatataag tgcaaataat atttccccgt
     5881 atctccaaat ggcgaccaag cacttccacc aaattttgat acccaatccg ttggctcttc
     5941 tcttaaaatg aagaagtcct gataatgctg gtcccctgct agtgcttttt tgaaccattc
     6001 atgttctgtc gaaacgtgat tcagcaccat atcgagcatg atttctatcc ggtatttctt
     6061 tgcttcggct attagctgtt cgaattcctc catcgtacca aagataggat cgatggctgt
     6121 gtaatcagag atatcataac cgttgtcttt ttgagggctt ggataaatag ggtttagcca
     6181 aatcatgtcg atgcctagat cattcagata cgggagtttt tctttgattc cttttaaatc
     6241 tccaattcca tcaccattac tgtctttgta agatttggga tagatttggt aaatgacttt
     6301 gtctttaaat gtcatggata tttacctcca ttcaactgat ttattaccta ccgcaactta
     6361 tatatacaag ttatcattta ttattataac tgaacagaaa gcgtttgtaa aatttatctt
     6421 tatacattct aagaggtctg ttgcccaata aaaaacaaga gatcagacat gtatttgtct
     6481 atcctcttgt ttaaaatagc aaaatattct tgtttcttct ataaatggtg ctcaaaagct
     6541 tttatctacg gcaatcagtc ttctcaaatg taaaacatga ggagctaatt ttatttgagt
     6601 attccttttg ctcgatgcag gacagctttt tcacaacctc tgattcacgg tgttcaatta
     6661 gcagctgacc ctcgttatta gctcaaaacg ctgatttcac aacctctgtt tttattcatt
     6721 cgatttcaac gcatcaatca tatcgatatg ctttaatttg aaatgggtaa caatcatcac
     6781 aataaccgta aatacgatag ttaatcctgc tgataagaca taagaccacc atgtgatcac
     6841 taatggaaag atgacattct ccatagacgc ttgttgcaag atgaagtcag tcaatatata
     6901 tcctacacca aatcctccga tgattcctaa aagagtaaag atgatatttt ctcgaacaat
     6961 gtacatcgtg acttctttat caaaaaatcc aaggactttt attgtagata attcgcgtac
     7021 acgctcggaa atattgatat tagttagatt atacaagacg ataaaagcca gcagtcctga
     7081 taatacgaca aagattaaga caaccgagtc taagttcgcc attgattctt cttgcgtttc
     7141 gatttgtttg gacatgaaag acgtatttgt cacagcgtct gtggcaagca gtttctcgga
     7201 aagcatttct tcttcttttg ttgtcatctt atctgtacgc agcaaaaatg cattatccat
     7261 catcgattga tcagtaactg aagcataata ttcaggggac atataaataa agctacctag
     7321 atagtttttc acgatttctt ttacttcgac tttccattct ttttgatctt catcataaag
     7381 cgaaatatga tctcctgtag ataagtcaaa tagttctgct gctttttgcg taatagcgat
     7441 tcctgtttta ggcaaggagc gtgggttctc ttcaagatca gttaaatgga taaaacgatc
     7501 caaatcagct gttttacttg gtacgatcac tgtcagactt tgcgaagcat gtcctttttt
     7561 tctcaattct acggtttgag tatgaatagc taggatctca tttacttttt cttgcttctg
     7621 cagcatatcc gctgcttttt gacgactatc ttccgtggat gaatccaatg taacgattgc
     7681 ttgatagtca atgatcggtc caaattgttt agctgagaca gaacttagag aatctttcaa
     7741 tcccacgcct gcaaccatca gcccggcaca tccagcaatt ccgataatcg ccataagcat
     7801 tcttgactta tatcggaaga gatttcggta actgattttt tgattaaagc taaggcgtga
     7861 ccacagcgga gtgatgtatt caagaaaaat gcgtttccct ggcttcggtg ctttcggttg
     7921 aagcaatgca gcaggccgtt ctcttaaatc tttgaataaa acgatcattg cagcgccaaa
     7981 tgcggcaatg aagaatgcgg cagctgcttg taaaatcggc ggccagtcat aaaaaataag
     8041 cgcactccca atatcatacc gctcgttaga cagctcaaaa atgatccttg gcaaaagatt
     8101 cgttcctaat atcgttccta aaacaatgcc tatccctgag gctagacttg catagattgc
     8161 atattttttt gcgatttcaa ctttcgtata acctaacgcc tttaatgtcc cgatttccct
     8221 gcggttttct tctaccatac gtgtcattgt cgtaaaggtg atcaatgcgg caataaagaa
     8281 aaagaaaaca ggaaaaacat tggcaatcgc agcgatacga tcagacagat caccaaattc
     8341 ttgaaaaccg acattttctg aacgttcatt gtataggtat gatggtttag ttaaattttc
     8401 aattttttct gcatttcgat tcaattcttc ctctgcagag gccagttcac gttctttctc
     8461 aactaattgc ctttcttgtt cagcaagttg agcttcttgt tgagataatg tttgaacagc
     8521 gattgttcgt tgcgcttcag gaagctgctg gatagctgct gactgttgcg ctaattgttt
     8581 tttagcatct tctaactgtg ttcttccctg tgctatttgt gctttgcctt ctgttatttt
     8641 ttgctgtgct ggttctaatt cttcgtttgc cgattttttc agctcttcta agcgttcttc
     8701 agggcgatct tgtaaatact tttctgcctt ctcttgattg tgttccattt tatctttgta
     8761 agctttgctg tacgtattca aattctttac gtttgtaaaa tcaagatata agacagaata
     8821 aatatctgtt ttaaaaacgg attcaggtaa atagacaaaa taatcgacgc taccatttcc
     8881 gacgttcgcg tatcctctct ccaaattgtt gatgaacaat ggagaacgga caaagcccgt
     8941 gatggtatac tctttttctt tcaattcatc tgaatcaatc tgataaatat caccgatttt
     9001 atacccttca tcttccgctt tttcatccaa tacaagctgg tttggttttt ccgggagttt
     9061 tccatcagtt aattgtagct tgttctgctt gttatttttg ttataagaaa atatttgaat
     9121 cacttcgttt ttaccagtat tggaataata gatctgtttt cctgtttcta cttttgcatc
     9181 tgggatcttt tctgcttgag caatatcttt ttgtgtcaag ccaccagtag aaacgatttg
     9241 caaatcagaa agtcctgctg taccaacata gtggtccatc gttttttgca tgacaggtcc
     9301 agcagttttg acacctacaa ataaaagtgt tcctagcatg acaataagga tgattgcaat
     9361 gaatcgtcct ttagattgcc gaatttcgcg aaaacctgct ttaagatatg ttcttgtttt
     9421 cataaaatcc ccctaccata caatctcatc aatggatgaa ggatgatcat ttaattcaac
     9481 agaccttact ttagcatcat taatgtgaat gactctatca gcgatgggcg ctaaagcaga
     9541 attatgtgta atgatcaaaa cggtattgcc gtgttgtctg ctagcgtttt gcaataattt
     9601 caatacttgc ttgcccgttt caaaatccag cgcgcctgtc ggttcatcac ataaaagaag
     9661 tttgggattt ttagctagtg cacgagcaat tgaaaccctc tgctgttcgc ctcctgataa
     9721 ttgggacgga aagttattca atcgatgagc taggcccact tgcctcaata cctctacagg
     9781 atcaagcgca tcaggagaaa cttccgttgc caattccaca ttttcttttg ctgttagatt
     9841 aggaacgaga ttgtagaatt ggaaaacgaa ccctacatct gttcgtcggt aagcagtaag
     9901 ctgtttatct gaaaattgcg caatatctgt gtcatcaata atgatctgac cttcatcagg
     9961 tgtatccatc ccacctaaaa tattaagaat cgttgacttt cctgctccac ttggaccaag
    10021 tataatcact aattcgcctt tttcaattga aaaagaaata tcgtcatttg ctgtaatggt
    10081 cgtttctccc attttgtatc gtttgtattc attttttact tcgacatact tcactctgat
    10141 tcctccattt tcacgatatt tctcgctata tctatgcatc gaatgcgttt tctcatactt
    10201 atttatttta ccactgaatt tctctagact aaagaaagga atcctttatt tagttgacaa
    10261 ataaacgatt tatgatttac ttacataaaa aacattgtta tttgaaagaa ggaacatcat
    10321 gaatgattta aaggaaaata tgatttttga tttatcaaaa actgcttttg tcgcgatcga
    10381 catgcaaaaa gggatcgtaa ataacggtac actctctcca cacactgcac aatctattct
    10441 gacaaacaat gatttgttag caaaaacatt gaaacgtacc gctgccttga aagttttagt
    10501 gaatgtagac atcactactt tctcttatct aagtcatcag acagatgctg gaggaatatc
    10561 tgctttgtct gctccggcag aattcactga cttgttgttg aaggattcct tgaaagatac
    10621 aaacaatatg ttgactatca caaaatacaa tccaagtgcc ttttttggta cttctcttga
    10681 tcttcaactt cgccggcgtg gaatcgaaac tatcgtttta agcggtgtag cgacaacaaa
    10741 cggtgtatat gctactgcat tagatgcctt ccaacatggg tatcatgttg tacttgctga
    10801 agatgcttgc agtgatcgtg acaaagaatc ccatcaattg ttcataaaaa agatatttcc
    10861 gaaaactgca cgagtacgct caacgaaaca aataatcgaa gctatccaac aaagcaaata
    10921 agtactggca cacgtgattt tcatttactt ctttttgatt tcatgggaaa ataaccacag
    10981 gatttagata ggagtatgaa catggtgaaa aaaaagatca caatcaaaga agtcgctaaa
    11041 ctttccggcg tttcaatcac aactgtctcc ttgatattaa atggaaatac ggagaaattc
    11101 agtccgaaaa ctgttcagaa agttttagct gccaaagaag aattaggcta tcagcctgac
    11161 tattttgcta gacaaatgat taccaaggaa acgaaaacaa ttggtgtact cgttcctgat
    11221 atcacgaatc cattcttcag tacactgatg cgaggaattg aggatatctt gtataagcag
    11281 aatttcgtca cgatattatg taatgctgat tctgatcatc aaaaagaaat cgaatatcta
    11341 gccgagctca cacgtcgagg tgtggatgga ttcattatcg cgacttctgc tgtctctaca
    11401 gatgcaatca acgaaaattt gaaaaaacaa ggccggccct tcattgtttt ggatcagaaa
    11461 aaatctgaag gatttagtga tgccgtacga acagacgatt ttcgcggtgg ttacctagcg
    11521 ggcatgcact tgcttagtct aggtcatcaa acgatcgccc ttgtctatcc tgaaaatccg
    11581 ccggaaaatg tccacgctcg gattgagggg tttaaatctg ctcttgatgt ttaccaaatc
    11641 tctcacgacc aactgatcct cttacctaca cagttctcaa aacaaggtgg ctaccaaatc
    11701 acagcagaac ttcttgattc tgctgcaact ggtgtgtttg ctttaaatga tgaattagct
    11761 tttggattat atcgcggatt agaggaatct ggaaaaagca ttcctgaaga ctacagcatt
    11821 attggctatg ataatataga tatgtgcgag tatatcaagc cgaaactgac aacgatcgca
    11881 caaccgatct tcgaattggg acaaacatct gccaaattat tgttagatcg gatccaattc
    11941 ccagaaaaag aatgggaaga aaaaagatta cctgtacgat tagaaaaaag attttcaact
    12001 gcacccttga aatagctttc aaggtgtgct atatttttat cgaactatta aaacgtttta
    12061 ctaaaacgtt ttaaaaaata atttttccca tcaaaataaa tgagatagtt ggaggtttgt
    12121 aaaatgaaca cggttacagt aattggaagt attaatatgg acactaccct tcgtttaacc
    12181 aacatgccca agccaggcga aacaatgcat gcgcatgaga tttttcacgc tggcggaggt
    12241 aaaggtgcca atcaagcagt ggcagccaaa cgttcaggcg cacggacatc ttttattgga
    12301 ggcgttgggg ctgacagcga aggacaacag ttattagatc tactgacaaa agaaaacatc
    12361 gatacatctg ggatagctga aatccaaggc gccacaaccg gacaagcaat gattatggta
    12421 gacgcagcag gagaaaatag tatcttgatc catgcaggtg caaataatgc tttccatgaa
    12481 caagaagtcc taaaaaacaa gcaactgatt acaaacagtg actttattat tgctcagttt
    12541 gaaagtagct tagatgcgac gatcctagct ttttctattg caaaagacgc aggaaaaaca
    12601 acgatcctga atcctgcacc agctcgtgaa acgatcccta ctgaactact tgaaaaaaca
    12661 gatatcatta taccaaatga aacagaaaca gaaatcatta caggaatcag agtgacagat
    12721 cataacagtt tggtcgcagc agccgaaaaa ctccatgaat taggtattgg aacagtaatc
    12781 attacactag gcagtgccgg cgctttctat catacagaaa aagaacacgg tatcgtccct
    12841 gcttttaaag tggatgccgt tgacacaaca gccgcaggtg atacatttat tggcgcatta
    12901 agcagcacac ttcagccaga ccttagtaac ttgaaagaag cgatccttta tggaaatcta
    12961 gcctcttctg ttgctgtgca gtcttatggt gcgcaacctt ctatccctta ccgcgaagct
    13021 ttagaacaat cagcagtcta aaattaacaa tgagaggaag aaacaaacat gaaaaaagga
    13081 aaagtaatca attcagatat ttcacgagta atcgcacaaa tggggcactt tgataaatta
    13141 agtatcgggg atgcaggcat gcctgtcccc tttggtagtg aaaaaataga tttggctgtc
    13201 tcaaacggtc ttccaagctt tttatctgtt ttagaaaacg ttttagaaga acttgaggta
    13261 caatcgattg aattggcgga agaaataaag acaatgaacc cggaaatatt ggaaaaaatc
    13321 aaattattgt taccagatac accgattagc tttatcccac atgaagaaat gaaaaaagag
    13381 ttgaataact gtcatgcatt tattcgtaca ggcgaaatga caccttacgc caatattata
    13441 ttagtgagtg gagtggtctt ctaatggaaa cgacaatgaa tcctgaacag atggtactgt
    13501 ccgagcagat tcatatgctg attgaacagt tgggaggaaa agacaatatt gattctgtcc
    13561 ttccatgtgc gactcggctt cgtatcaaat tgaaaaaaca gccagtagcc aatgcagaaa
    13621 aattaaaaca gcaaatccct tccattaagt atttcatttt actcgataga tatgcagaaa
    13681 taatcatgga tcagggattg gctcctgctt acaaggaatt aacgcgtcaa atcgatcatt
    13741 cagctcctaa agaaaagcta aatccgatcg aaaaactatt tggtttgata tcggaaatct
    13801 ttacgccgat cattccgctg tttgccggtt ctggtatatt aaaaggtctg gttgttttag
    13861 cgactacgat tggctggctt agtgaaacaa gcggaactta tcatattttg tctgctgcgt
    13921 cgaatgccgt cttttatttt ctgccaatct ttcttgcttt ttctgccgcc aaaacgttta
    13981 aagtgaatgg atatattgcg gctgtcattg ctgcagcact gattgaaccg aatctgacct
    14041 ctttactttc agacagtggc aaagcagcta ctgattttct ggggattcct gtagtactga
    14101 tgcagtatac gtctactgtc atgccggcga ttttaggtat tttcttttat tcttttgttg
    14161 aaaaattcct caaaaaatat attcgcgaaa atatgcaatt agtatttgta cctttacttt
    14221 cattagttat cacggttcct ttgacgttaa tggtattcgg gcctgcgggt gtttaccttg
    14281 gcgaaggttt agctgccgca attacttgga tgatggatat taacggaccg atcagcggcg
    14341 caatcatcgc tggtggttgg aatattttgg taatatttgg tatccaatgg gctgtaaacc
    14401 ctatcatgat cagtaatatc tctgcttatg ggttcgaccg aatcgttccg ttgacaggag
    14461 cagccaattt tggaatggct ggtgcagctt taggtgtatt tctacgaagt aaacgttcaa
    14521 aaactcgttc gatctccggt tctgcttttg cttcgatttt acttgcggga gtgaccgaac
    14581 caactgttta cggaattgcg atcccattga aaaaaccatt tgttgctgct tgcattggtg
    14641 cagctgctgg tggagctgta atgggatttg cccaagtgaa agcgatcgct tttgtatttg
    14701 gtagtctgac cacattgcct gcattcattt ccggtacttt tttctggtac ttagcaggac
    14761 tggctgttag tctagtcgta gcaatgatta ctactctcgt tagcgggttt gatgaagacc
    14821 taatgtctta tgaatagtta atgaaaaaga ctgaagtatg agatgtttat cacatatttc
    14881 agtctttttc tttttaggat cattattttt ataaattttt gtgatacttt ccaaactttg
    14941 gtataattta catatgcata accattttta tccccacttg tttatcccca aacaaaggtg
    15001 gtctatgcaa taaaatgaac gtcaatgaaa aacgaatgaa tgctcgttgc ttatttacaa
    15061 attgttttat ttagaataca cagacagatt tatgaaaaga aaaagctttt cgacacgatt
    15121 atctgttcca aaaaatgtct aatgaatgct gaataagaca gaaaatcaat gaaaggaaaa
    15181 ggactagttt attatgaaaa ataaaaaaac gatacgtttg ctagctgcta cttggctgat
    15241 tgctccgtct gttttgtcga ccaataccgt atttgccgta gataatcaaa cagctgatca
    15301 aacagtcaca acagatacga ctacatccag ctcagctact agtgaaacaa cggattcttt
    15361 tacagaagac ccaactggaa caactaactc aacaacagga tcgaccgagc caactgaaat
    15421 tgatccggct gatccatcag aaaatgaaaa gccagccgag acatattccc ttacaattgc
    15481 tccttctttg attggtaaga tccaactgac tgcttcgaca gcggattccc ctgaacagca
    15541 acctttggat attacggaaa gtggaacggt agaaggttta ttagcggaaa cgaaggtaac
    15601 ttacactatt acgccagctg aaggtgaaca aattgcttca gtcgctgtta atggtgccaa
    15661 agaagtaaat tatgatgcaa agacctttac agtaggtacg aacaatttat ctattactgc
    15721 agaatttgaa cctgtaccgg atacttcaac agagaaacca actgatccta gtgaagaagt
    15781 ccctagtaca ccagatccgg aaacgccaaa caaaccagat gtgggtaata acaacaataa
    15841 taatggttca aataattcaa acaataataa caataattcg aacagcggga attcttcgaa
    15901 tcaaacaaac ccatctggat cgaataacaa tggaaatact cagagagaac caaaccatcc
    15961 aaacaatggt tcatcaaatg gcggaacgaa caatgcttca tctcgtccaa atacagcaaa
    16021 tcaaagcggt atagagaatc catcttctgc ttcttctgat tttgttgtaa aatcaccaat
    16081 tgatgctgta ttgcctacaa atacaacgaa tgtccaacaa gcaatcgttc gagaagcatt
    16141 caagcatctt ggaaaaccat atgtatgggg agcaaaaggt ccaaatactt ttgactgttc
    16201 tggtttgact tattacgtat atatgaaagc aaccggtcat tatatcggtg gctggacagg
    16261 cgaacagcaa tatgcaggta cacagattcc agtaagtcag gcccaacctg gtgatctcgt
    16321 cttctgggga ccttcttctg gtgttaccca ccacgtaggt atctatattg ggaacggaca
    16381 atttatccat gcgcctcaac caggagataa agttcgagta acttctattt cagatttcac
    16441 gcctgatttt gctgtccgag taaacttagc aggtttacct gctgcttcag gttcgctgac
    16501 aggcggctca tctattttag atggattaga tggctcgttc catttcagtc aaaaccaaac
    16561 aactgatcag ttcttgaaaa agattgcaga tgatgcacag gaaatcgggc aaaaagaagg
    16621 aatctacgca tcagtcatga tggctcaagc tatccttgaa agtggatctg gaaacagctt
    16681 attatcaagc gaaccaaatc ataacctttt tggtatcaaa ggatcatata aaggaagcag
    16741 cgtgaccttc aatacacttg aacaagatag ttccggacaa agctaccaaa tccgcgcaca
    16801 attccgtaaa tatccttctt acaaggaaag tttggaagac tatgccgact tgatcaaaaa
    16861 tggactgact ggcaatcctg atttctacaa accaacttgg aagtcggaaa caaaagacta
    16921 caaagaagca accaaatatt tggaaggacg ttatgctaca gatcgccaat attcacaaaa
    16981 attgaacgcg atcatcgaag cgtacgacct gacaaaatat gatgaaccga aaaaagaagc
    17041 agcaacaact gaagaagcaa acgctgaaat cctcaatatg tctaagcgat tcgatgtgcc
    17101 aatcaaatgg acaacagatg gcgtgccaat cacaggatta gaattttcaa cgataaaacg
    17161 cgtcaacaac gcttcttcca tcttccgtgt cgcttcaatc tgggatttgt ggaacaattt
    17221 tacggcaaga cagataccag aaacaactac tcgtacagtt aaagctgcaa ctgttcctgt
    17281 actaaccttg ttaagtttag acaaatcatt ctggcttgat ctgcctttaa agaaatagaa
    17341 taaat
//
LOCUS       DAHEYX010000042        17258 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHEYX010000042 DAHEYX010000000
VERSION     DAHEYX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.042"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(27..572)
                     /locus_tag="MB219_000957"
     CDS             complement(27..572)
                     /locus_tag="MB219_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCC8426689.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(584..1276)
                     /locus_tag="MB219_000958"
     CDS             complement(584..1276)
                     /locus_tag="MB219_000958"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCC8426690.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1295..1579)
                     /locus_tag="MB219_000959"
     CDS             complement(1295..1579)
                     /locus_tag="MB219_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426691.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1592..2155)
                     /locus_tag="MB219_000960"
     CDS             complement(1592..2155)
                     /locus_tag="MB219_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCC8426692.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2383..3045
                     /locus_tag="MB219_000961"
     CDS             2383..3045
                     /locus_tag="MB219_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCC8426693.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3169..4365
                     /locus_tag="MB219_000962"
     CDS             3169..4365
                     /locus_tag="MB219_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCC8426694.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4466..5842)
                     /locus_tag="MB219_000963"
     CDS             complement(4466..5842)
                     /locus_tag="MB219_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8426695.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5879..6745)
                     /locus_tag="MB219_000964"
     CDS             complement(5879..6745)
                     /locus_tag="MB219_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCC8426696.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6834..7859)
                     /locus_tag="MB219_000965"
     CDS             complement(6834..7859)
                     /locus_tag="MB219_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCC8426697.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7877..8551)
                     /locus_tag="MB219_000966"
     CDS             complement(7877..8551)
                     /locus_tag="MB219_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426698.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8735..9628)
                     /locus_tag="MB219_000967"
     CDS             complement(8735..9628)
                     /locus_tag="MB219_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCC8426699.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9702..11399)
                     /locus_tag="MB219_000968"
     CDS             complement(9702..11399)
                     /locus_tag="MB219_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCC8426700.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11392..12705)
                     /locus_tag="MB219_000969"
     CDS             complement(11392..12705)
                     /locus_tag="MB219_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCC8426701.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12705..16742)
                     /locus_tag="MB219_000970"
     CDS             complement(12705..16742)
                     /locus_tag="MB219_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCC8426702.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16755..17213)
                     /locus_tag="MB219_000971"
     CDS             complement(16755..17213)
                     /locus_tag="MB219_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCC8426703.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4829 a         3885 c         3008 g         5536 t
ORIGIN      
        1 ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt ggactcaaaa
       61 tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggaatc tttgaaaata
      121 tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca gatatctgta
      181 catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat gtctagatct
      241 gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc ctcttgctca
      301 tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt tcctatcaca
      361 ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc atgcgcatca
      421 atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc agtcagcttt
      481 ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc aacaaaatca
      541 tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg agatgtttga
      601 caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat tcgtcaaagc
      661 tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa aatgggatat
      721 tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct aatgcttcag
      781 ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta tcacctgtaa
      841 cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc atttcactgc
      901 gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct gggttgtagc
      961 caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg atgtcaccta
     1021 cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg ttcacgccgt
     1081 attgttggat caacagactt gtggtaatag atgctaagac ttttcctata ccggaacgta
     1141 cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct gcgcgttccc
     1201 atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc attgcgccga
     1261 taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt gctaaaaaaa
     1321 ccacacataa cattaggata acaataataa gagatttcat caaaaaagag ttccatttgg
     1381 tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt ttgctttctt
     1441 tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct gcttttcgct
     1501 gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg cttcgcgtaa
     1561 taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat agttcccagt
     1621 attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc gcttgttgcg
     1681 cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt gctaaaggat
     1741 tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt gcaaaacctg
     1801 gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca gtttcttctt
     1861 ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg atttttccag
     1921 ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca agaagcagac
     1981 gatcttgttc gtcaaaagca atgatcccga caccgccagg atgaaatacc agttctctct
     2041 tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata atttttccgc
     2101 tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc agcatatctt
     2161 ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt tctcaaaaac
     2221 tccttattag tttaccatag ataaacctta gattttctct aaatatagaa aatccttctt
     2281 tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc gaataacgaa
     2341 aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa acgaaattgg
     2401 ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag tttttctttt
     2461 gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt tggttcgcgc
     2521 aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga aactcactat
     2581 gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat gaatcaaaca
     2641 aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact taaagcaatc
     2701 gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt ggtgaaagaa
     2761 ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa tctagttgat
     2821 ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca gacctatgaa
     2881 aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc acaagattat
     2941 caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt tgcgaaacag
     3001 agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt ttgttcataa
     3061 aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt ctaaagtaga
     3121 gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat ggatcataaa
     3181 ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt attgaacaac
     3241 ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat tgatcagctc
     3301 caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg ccaagaacaa
     3361 gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat cagctacgga
     3421 tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca tgtgcaagca
     3481 caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct ccaagaagcc
     3541 aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt cgggaaggtc
     3601 aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca aattgacaag
     3661 atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct gatgttagaa
     3721 cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat cgctgctgga
     3781 gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa aagagcagaa
     3841 gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt cctagatcgt
     3901 ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca gcaagcacca
     3961 caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca agcatctatc
     4021 gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt gcttcgtcaa
     4081 aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt attgaagaaa
     4141 aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga acgtggaatc
     4201 gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat ccaagagaca
     4261 ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga gcttggacac
     4321 atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac ctttcgcttg
     4381 ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt ttttcttatc
     4441 tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt ccatagccat
     4501 agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc catgtcacag
     4561 tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc aatagcggaa
     4621 cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc aacttcagta
     4681 agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt tccgctaata
     4741 cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca taagtcgctt
     4801 ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt aagccatcct
     4861 tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt accgtcagta
     4921 gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct acatacctca
     4981 ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt cctgtcaaga
     5041 tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg attcctcttg
     5101 gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca tcaaatccca
     5161 agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca aaaggcatga
     5221 atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac aacgcgatga
     5281 taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg gcttcaagcg
     5341 acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg tatgtaccat
     5401 tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa cttttcaaaa
     5461 aaccttgcat atagccagac catccggaag caacagagga aaccgctagt aaaaattccc
     5521 cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca tacagatagg
     5581 catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt agtccagaca
     5641 aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca gcattttgat
     5701 tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca attccaagca
     5761 tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg agattgatct
     5821 ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa aacagcgatt
     5881 aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg caaccaacaa
     5941 taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc ccacctaaag
     6001 tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca agtgatttga
     6061 ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc atgtttttcc
     6121 acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct aagatcattc
     6181 cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg acgatgatgg
     6241 tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta ccaaaatcaa
     6301 gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc aaatacgcac
     6361 cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt tgtgtccatt
     6421 catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca gtagacaatg
     6481 gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt tgtccaacac
     6541 tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt acaggactaa
     6601 caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct agtgtttgtt
     6661 gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat gccaacatag
     6721 ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg ataagaaact
     6781 tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat tttctaacgt
     6841 ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc ttttccttca
     6901 gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc taaataaagt
     6961 cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa tcccttatca
     7021 aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac tgaaaggctg
     7081 actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg cgtatatttg
     7141 atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc ggtcaaaaca
     7201 gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata atttgcctga
     7261 ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag gtagtcccgc
     7321 gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc gatttttgat
     7381 aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat cgaacgaact
     7441 agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc aatataatct
     7501 ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc acggatcagc
     7561 tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact caaataattt
     7621 tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct cttgctcaag
     7681 taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa ataagcaatt
     7741 gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt caatgcaata
     7801 aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa aaaattcatc
     7861 ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt tcgatatatc
     7921 cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact gtcaatgtat
     7981 tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc cgacttaaga
     8041 tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg ctcaaatcaa
     8101 tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg ccattgtggc
     8161 tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat gcattgattt
     8221 ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc cccatgttca
     8281 tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc ggtactttcg
     8341 atacttcacg tattttttga caccaataat agccatccaa aacgggaaga ttaatatcta
     8401 gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg aaatcggtcg
     8461 ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt cgaatgatct
     8521 cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt cttcttgcta
     8581 ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat gtcattttag
     8641 caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt ccaagcagct
     8701 taaatttatt atgttattct taataattgg tctattatct gttatttttc tttcgttgtt
     8761 tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact agcaatatac
     8821 ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct atctttgaag
     8881 agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga gtgttcacgg
     8941 tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt gctaatgtat
     9001 ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc atccgaccat
     9061 acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta aagagttttt
     9121 tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct ccagttggaa
     9181 aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc gtttcatcaa
     9241 ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata tgttgttcgt
     9301 aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga tctttgactt
     9361 gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt tcatgcactg
     9421 ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga taattctcga
     9481 tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat gtaaaaactg
     9541 cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg atttgggttt
     9601 gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa aaaactgcag
     9661 caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc agacttcatt
     9721 ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat tagtaagaat
     9781 gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt cggtactctt
     9841 tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct tccataagag
     9901 atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc tggcgccatg
     9961 gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta tccatttcca
    10021 tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc tgctcctgat
    10081 tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga acggtcttca
    10141 ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc atatttttca
    10201 aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc ataatcaaat
    10261 tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc cgcttctagt
    10321 tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc atctgctaat
    10381 ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt cacgacaaac
    10441 ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt ctcgccggct
    10501 cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc tgtgattttg
    10561 taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata aggaataggt
    10621 gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga aatcgtttct
    10681 tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc tacattcatc
    10741 tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt attgtatgtg
    10801 tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat cactggcatc
    10861 ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt ttctaagaaa
    10921 agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt agagaatacc
    10981 gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata ttttaaaaac
    11041 caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt aagaccgtct
    11101 tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc cgttacgtca
    11161 aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga aaccaatccg
    11221 ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt aatcgtcact
    11281 tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc gaaaatccct
    11341 attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt tttattcatt
    11401 tttttctccc cgttcttttt ttattaataa aacagcacct aacaataaaa gaattcctag
    11461 taaagaaatc aacgttcttg cttcattcgt ttgcgggaat acggaccatt ttcctgattg
    11521 acctggattg cttggaccac tcggctcttt cggtttgtcc gttgtttctt ttacggtctc
    11581 gttataaaca cgtggtgttg cggattcata ggcagagact ggaactttcc cggattctaa
    11641 ttcagccatc tcctgtccgt taactactac atattgtggt tggccttttg catccaccca
    11701 aaattcaggg atgatgacct cgattctctt acttgcttca tccatctcgt aaccttccac
    11761 accttttaat tcttcaaaat aataggtgcc agaaggcaag aaacgttgtc ctgtcgtaac
    11821 aaggccctct ttatccgaaa tgaatttgct tacatttttg tcattcaaag gatcattgtc
    11881 tgctggtttg atccattgat ttttcaagtc attagcagga gccatatcta aataatattt
    11941 ccccccttct atcatctgat atagagcaaa aacagcacct tctaatggtt ttcctttttc
    12001 agttgttcct ctttgacgtc catacttgaa gaagtatgga tctcttagat aaccgacatt
    12061 tttaggatag atatgaatct cttgcagttc ctcggttgat tctgtcgggt gataaattgg
    12121 caaaatagca gcaattggga tagctgtctg atcgatatca acatccaatc caacttctgg
    12181 atcaatcgat tcttcgaaaa tcaaataaac tgcatcccga tcttggtttt tcttaggtgc
    12241 tttgatacgt gcaatcccgt ctcctaatcc gccaaccgta tctttcccag ttgttaccga
    12301 agtccctatt tggacaagcc tttcttctgt gattagctga cggatattgt ttctgttcgt
    12361 ttcagcgatt tgctggacga attctttagg agtcatgttt tcagcagctt ttttttcatg
    12421 ataataatcg gtcatgtcgt aaatggcaaa tgtcgctcca tttagtggca ccgtctgtga
    12481 aatgatatct gctgtttccc ctgcttcctc agagatagcc aagccatcat tttcatggta
    12541 ttcaaattga tcgttataat cgatatctcg aatcatccgt ttatgcaaca cgaattccac
    12601 gacatcttcc cctgattctt ctgccgacat cgcatgtgga agaaggactg ctgcaagcgg
    12661 tagtaaaaca agtaattgaa gcatcaaaca caacagcttt ttcattacct tgctccctcc
    12721 tttttagatt gtctgacaaa caaaccatag gctgctcctg caagcgccat gaagaatgca
    12781 gcaagcagga tatagaataa tcgtccggat cctcctgtag caggcaacgg attttcagga
    12841 tcgttcccga cactaagctg taatctgatt cgatttgtct ccaaaagttc atattctaac
    12901 tcttgctgta gatcaggcgt ctctgcttct tcgtttggat aatacgaaat ctcaactttt
    12961 agtgtacccg cttcttttcc tggaactacc cgatcatttt ctatgtcagg ttcttctcga
    13021 taactaattt ctaaaataaa gtaacctgga agttttctat agctgtcagg tgacttcact
    13081 tccttgatcc gatagacccc ttccaacaag tctgaagcat tgaataggga agaatcgttt
    13141 ggatctggtg ccaagcctcc ctcgatgatt tcatactctc ctgcactatt ttgtttctca
    13201 attgaaaatt ctgcgccttc aagacgtcgg ctattcatac tgtcaatttt caaaagttcc
    13261 aaatcgattg gcgctagttc atcttctaag gttacttgta gtacaccatc gatgatttca
    13321 tagccttccg gcaagggagc agtttcgtct tcgatgaccg tatcttcaat gataaactta
    13381 ccttcttcat tcagtgcaaa ctggaacact tgatcagata gccgataccc atttggtgcg
    13441 acagattccg agacctcata ttcaccaata gacagatgca gaggttggtc gtaatttaaa
    13501 tcgacatact tttctactcc tacggcacga gaatagaagt atccgatccc aggcaaacgt
    13561 ttcgattctt ctggttctaa cggattacca gtgtatgctc gatacagcgg gaaggaagaa
    13621 tcccccactt tcctgatatc gaaacctgca ccatctaatg tatggccttc atggtctaat
    13681 tttctaactc ggatctcccg ataatcaaac gtattgtaag cagtgaatgc tacccataaa
    13741 cgatcctctt catctgttgt atacgtgaga tattgccaat tttcttcacc tgtttgcttg
    13801 agttggatag tccctatagc attatcgtaa agtcgcaaga tccatgtgtc attttcatca
    13861 ggttctttga aaccttctgg gttctggctc tcaatgattt tgtattctgc atagttggta
    13921 gtttcttcac tatccttata aacaaatgaa tctggctctc cgattggatg aataactttc
    13981 tcaccatcca cgattacata tttcccgtct tcatctactg aatagaacgt gagtctccct
    14041 ttctcttcct ctacttcgtt cacaccagaa agccctttcg ctactggata tccgatttcc
    14101 agtccgttac catctgtttc ataaagttca aactctgcgc cattgattgg catattactg
    14161 ccttcaatta gtttattgat attcatgtgg attggcttga attcatttcg catttccagt
    14221 ttgacataca actcatcata ctctgtgcct tctatgttag gctcttgtac aacagaaagc
    14281 ttgtgatatt ctccttcata ataggaattc ggatctttac cttccggtcc gccgactctc
    14341 gaaccagatg tatattcatc tacaagctcg tagtgaccta acatctcatc ttcgttggct
    14401 gcatattttt tgatttggaa ttgatacgtc tcatctacta actgataacg tggtggtcca
    14461 gcgatctctc tgatccaaaa tatgtcgctg actgctccag tttcatctcc taaaatcggc
    14521 atcttcgttt caaagatcgc tattccgtcc gtggtagttt tacttgtttc gattggttcg
    14581 gcttccccac tctctggatt ttggtcatat aatgcaaatt cgactccagc caacggctcg
    14641 ccattttgac ccttcttgct gacttggaaa ttacggcctt tatgttcgta acgaatggaa
    14701 gaagatggga aatacatccg tccagtagtg atatcattct cataatcctc caggttctga
    14761 atataggcca tattgttcac tgtgcaaaaa tttccgtctt gagctcccgc atcaagtttt
    14821 acttggtact tgatcctgac catttcccct tcacctaaag aaatcggaga aaatttataa
    14881 gcttggtttt cccgtccgac atacgttgtc ggagtcgcca ctacttctgg tggggcttgt
    14941 ccatgacgtt cttcatccca tatttctggc tcttggtact tcatgccgtt ttctcctgga
    15001 tctagatggt aaaactgaat atctgccgat ctgccagctc caaatggtga gacattaaaa
    15061 ccagtttcat cataaagtgt ggcttgctgg atcgttctct tgaaggtaga agagatatcc
    15121 tccaaggctg taacaatgtc attcttgttg ttcgcattgt aatatcgcgg tgttacgcca
    15181 tcttcttcta aatcagcaat atttttcaat acatttcgtc caatagcatc tagtcgttgt
    15241 ctcccgctaa cgtttccatc gattcctaaa ccaatggaga agatacttgg cgggttctct
    15301 tgatacttat ttttcagcca atgatgataa gcaactgtgt tgatcgcaga tgacttttct
    15361 tgcgaagtag gactgccatt cgtattgtta ttagaagaac catcaccaac ccagtgagtc
    15421 gctttattat ctccttcttt tacctctcca taacgatagt gtacatttac ttcatcttct
    15481 ggacggttaa agtcagatgc ataggtaact ggatgagtaa accctgcatt agaagtattc
    15541 ccagaaaact cctcaaaatt tctgaacaaa acgtcattat cttcagcaat cttatcttcc
    15601 atcacagtcc agttgctcca agctccatta gtgctcgtac gataacttga ctgaatcggt
    15661 gcataactga aggtcggtgt cccatctccc acaacgatta ggattttttc tggatttcta
    15721 ttttctccgc cattgtcttt atacaatgtt tcatagccat ttttaagccc tagtgtaagc
    15781 ggagttcctc cgattggtgt acgatttatt gctgtattct cacttcccgt caaacgattg
    15841 atatcatttg tcaatggaaa gatgtccgaa ctgatttgct catggttatt gatagctgta
    15901 ctattatggt aaaaattcac cataccgatt cgaatatcca gactcgggtc agagagcaga
    15961 tctttactca tttcgtttac tgcctcaatc aacgcagcat ttttagtttg actattggtc
    16021 ccagcggtta attcactcat actcgctgac ttatctagaa caaaaacgat atcgattgga
    16081 ctgatttcct ttttctcgcc cccgatgaca tctagataga cattgtaaag ggttgttgca
    16141 tcttcaggat gtagatcaga tgctgtgctg cttggcagaa caggttctac ccattttttg
    16201 atgtacgccc cgttataatc caagtaactg ttttcaaaat tccgttcatt tccccaaagc
    16261 tgaacggttt gagctggaac gtttctatct tcttcgcctg cagttgatcc attattagta
    16321 gcatagtcgt aatttcgcca gttcgctccc ccgtttgtat aatcactatc atcgatcaag
    16381 ttctctccgt catatggcga gcctttgtct ccttgatagc gttcattgct ttgtgtccaa
    16441 tttcctaaca cttttgtata gtcattcgtt gtaaacttcg ggtagcgatg ctgttcgttt
    16501 tcaacttctc cgctaggata gatatattca taggggtctt gcgtagctgt tggcccaaga
    16561 cgctccaaaa taccgttgaa taactggcgt tgaatgcgct ggagtgcttg accgctgggt
    16621 acgattggtt cgtccagctt ttccatctca ttttcttcgc tacgttgaat ttcagcggaa
    16681 tcttctgtat cagtttcatt ttcttgtaat tcttggttat ctattctttc atcaaccacc
    16741 acttgaagag gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat
    16801 gtgccggttt ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc
    16861 gtctcgactt ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg
    16921 ttttttacgg taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca
    16981 ttggcacttt cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata
    17041 tacttccctt caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct
    17101 gttgactcag cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca
    17161 aatagcaaac tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta
    17221 tactcctttc aaaaatattt gtcttctttc acaacaat
//
LOCUS       DAHEYX010000043        17220 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHEYX010000043 DAHEYX010000000
VERSION     DAHEYX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.043"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            48..1811
                     /locus_tag="MB219_000972"
     CDS             48..1811
                     /locus_tag="MB219_000972"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCC8426704.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            1895..2353
                     /locus_tag="MB219_000973"
     CDS             1895..2353
                     /locus_tag="MB219_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCC8426705.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            2462..3145
                     /locus_tag="MB219_000974"
     CDS             2462..3145
                     /locus_tag="MB219_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCC8426706.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3292..3906
                     /locus_tag="MB219_000975"
     CDS             3292..3906
                     /locus_tag="MB219_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCC8426707.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            3922..4128
                     /locus_tag="MB219_000976"
     CDS             3922..4128
                     /locus_tag="MB219_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426708.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            4667..5224
                     /locus_tag="MB219_000977"
                     /pseudo
     CDS             4667..5224
                     /locus_tag="MB219_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            5282..7321
                     /locus_tag="MB219_000978"
     CDS             5282..7321
                     /locus_tag="MB219_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCC8426709.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(7430..8581)
                     /locus_tag="MB219_000979"
     CDS             complement(7430..8581)
                     /locus_tag="MB219_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCC8426710.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(8735..9265)
                     /locus_tag="MB219_000980"
     CDS             complement(8735..9265)
                     /locus_tag="MB219_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCC8426711.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(9364..9714)
                     /locus_tag="MB219_000981"
     CDS             complement(9364..9714)
                     /locus_tag="MB219_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCC8426712.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(9871..11400)
                     /gene="ahpF"
                     /locus_tag="MB219_000982"
     CDS             complement(9871..11400)
                     /gene="ahpF"
                     /locus_tag="MB219_000982"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCC8426713.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(11410..11973)
                     /gene="ahpC"
                     /locus_tag="MB219_000983"
     CDS             complement(11410..11973)
                     /gene="ahpC"
                     /locus_tag="MB219_000983"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCC8426714.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(12117..12779)
                     /locus_tag="MB219_000984"
     CDS             complement(12117..12779)
                     /locus_tag="MB219_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8426715.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            12963..14609
                     /locus_tag="MB219_000985"
     CDS             12963..14609
                     /locus_tag="MB219_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCC8426716.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIVEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            14600..15682
                     /gene="uxuA"
                     /locus_tag="MB219_000986"
     CDS             14600..15682
                     /gene="uxuA"
                     /locus_tag="MB219_000986"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCC8426717.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(15790..17220)
                     /locus_tag="MB219_000987"
                     /pseudo
     CDS             complement(15790..17220)
                     /locus_tag="MB219_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         5851 a         3069 c         3298 g         5002 t
ORIGIN      
        1 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
       61 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
      121 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
      181 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
      241 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
      301 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
      361 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
      421 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
      481 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
      541 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
      601 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
      661 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
      721 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
      781 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
      841 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
      901 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
      961 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
     1021 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
     1081 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
     1141 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
     1201 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
     1261 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
     1321 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
     1381 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
     1441 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
     1501 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
     1561 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
     1621 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
     1681 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
     1741 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
     1801 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
     1861 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
     1921 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
     1981 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
     2041 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
     2101 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
     2161 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
     2221 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
     2281 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
     2341 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
     2401 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
     2461 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
     2521 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg
     2581 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
     2641 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
     2701 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
     2761 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
     2821 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
     2881 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
     2941 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
     3001 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
     3061 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
     3121 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
     3181 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
     3241 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
     3301 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
     3361 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
     3421 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
     3481 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
     3541 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
     3601 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
     3661 ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
     3721 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
     3781 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
     3841 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
     3901 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
     3961 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
     4021 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
     4081 atgccagatc aaacgattag cggattgttt aattacttta acaaataaat atttattccg
     4141 aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa gaatcctggt
     4201 gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt ttctaaaaaa
     4261 tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa tcattaggaa
     4321 aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg aggctcctat
     4381 acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt
     4441 tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata gtgctaaaac
     4501 tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct gaccttttct
     4561 ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct gacaagtgga
     4621 ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggaggtc gctcggattc
     4681 acttctggtt gcacaataca accagaaaac acaacaagca aaactgatct ctatcatgag
     4741 agattcatat gtcgatattc caggttacgg aatgaataag ataaatgcag cttactctta
     4801 tgggggagtt gatttattaa atcaaacatt aaaggaaaat ttcaaatttg aagctcccta
     4861 ttatgcaagt atcacatttc aagattttat cgattgcgtt aatgaactgt ttcctggtgg
     4921 agtcaagatc gatgcagaaa aatctctaga tttagatggc gtatatataa acaaaggaga
     4981 gcaagtgatg gatggtaata ctttactcca gtatgctcga tttcgggaag acaaagaagg
     5041 agacttcggg aggatcagaa ggcagcagca agtgatcaaa gctatctctc agcagctgaa
     5101 agacgtaact tcaatattca agctacccaa agcggttggg aaattacttg gtagtatcca
     5161 aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg gatttcctca aagacatatg
     5221 ttagaataaa caggtataaa tagtgaaata aaggaatgac aagcaagaga aggaggaaaa
     5281 aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc gcttatattg ccggcgcagt
     5341 gatcttaata gcagctgcgg gtggcggtta tttttactac cagcactatc aagaaaccca
     5401 agcagtagaa gctggagaaa agacagttga gcaatttgtc caagctttga acaagggaga
     5461 ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca aataaaagtg cattatctga
     5521 aaaagagatc ttagataaat accagaatat atacggtgct gctgatgtca aaggacttca
     5581 gatatcgaat ctaaaagtag ataaaaaaga cgattctact tacagttttt catataaagc
     5641 aaagatgaat acctcattag gtgaattgaa agatctttct tataaaggaa cattagacag
     5701 aaatgatggt caaaccacga tcaactggca acccaacttg gtttttccag aaatggaagg
     5761 aaatgacaaa gtaagcctga ccacacaaga agcagcaaga gggaatatta tagatcgaaa
     5821 tggtgaacca ttagcaacaa caggcaaact aaaacaatta ggagtcgttc caagtaaact
     5881 tggagatgga ggcgaaaaaa cagccaatat caaagcgatt gcttcctcct tcgacttaac
     5941 agaagatgct atcaatcagg cgatttcaca aagctgggta caacccgatt actttgtccc
     6001 attgaaaatc attgatggag caacgccaga acttccagct ggagctacca ttcaagaagt
     6061 agacggcaga tattatcctt tgggtgaagc agctgctcag ctgattggtt acgtgggaga
     6121 tatcacagca gaagatattg ataagaatcc agaattaagc agtaatggaa aaatcggtcg
     6181 ctcaggtttg gagatggctt ttgataagga tcttcgtggg acaacaggtg ggaaattaag
     6241 cattacagat gcagacggtg tagagaaaaa agttctgatc gaacatgaag ttcaaaatgg
     6301 caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa atagcttttg acagtctagg
     6361 aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc ggtgatcttc ttgcgcttgc
     6421 tagctctcca agctatgatc caaacaaaat gacaaacggg atctcacaag aagattacaa
     6481 agcttatgaa gaaaatcctg aacatccatt catcagccga tttgcgacag gttatgctcc
     6541 tggatctacg tttaaaatga ttacagcagc aatcggtctc gacaacggca ctatcgatcc
     6601 aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa gacagttctt ggggatcttt
     6661 taaaacaacg cgtgtcagta gtgatgtatc acaagtagac ttaaaaactg ctttgatcta
     6721 ttccgataat atatatgcgg cacaagaaac gttgaaaatg ggtgagaaaa aatttcgtac
     6781 aggcttagat aaattcattt ttggtgaaga ccttgatttg ccaattagta tgaatccagc
     6841 acaaatttct aatgaagata gctttaactc agatatcttg ctagctgata ctggatatgg
     6901 acagggcgaa cttctaatta ctcctatcca gcaagcagca atgtattctg tttttaccaa
     6961 caatggcaca cttgtctatc ctaaattgat tgcagataaa gagacaaaag ataagaagaa
     7021 tgtcatcagc gaaacagcag tacaaacgat cgtgccagat ctgagagaag ttgtgcaaga
     7081 tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag cgaaaactgg
     7141 tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa gagaacagtt tcttgtttgc
     7201 tttcaatctt gataaccaag gttatatgat ggtcagcatg ttggaaaata aagaagatga
     7261 tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa attatcaata
     7321 aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag tccttttttc
     7381 aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact cacacttcat
     7441 aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt aataaaatca
     7501 cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt ccaatttgta
     7561 aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc aatgaactat
     7621 gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg gtcaaaatcg
     7681 ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa aaatcaactt
     7741 ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa gcctctacat
     7801 tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca aaaaaagcac
     7861 caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa caaataacta
     7921 gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa agtaggaaag
     7981 gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt tcaatcaaaa
     8041 caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg ctgaaactta
     8101 gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt gatccttctt
     8161 ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca gaagttgccg
     8221 caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct ttgcctgtca
     8281 accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata ccaggtcgga
     8341 aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt aataagatca
     8401 caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc aatccagctt
     8461 ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct aatctccgcg
     8521 agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca ataaattcca
     8581 ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac attattgaaa
     8641 aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt ggattgttgg
     8701 tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt tttcttattc
     8761 tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg aatatttgga
     8821 atagaagcat caaacttttg aatggattga acaataagac gaatccgcca atcaacaaca
     8881 gaatactact ataaacactc caacttttag cttttgtatt catgctgaag aacaactgga
     8941 acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa acgattgcag
     9001 gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg aacagaccga
     9061 tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc aacaacccaa
     9121 gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca gaagggttta
     9181 gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg tatttttgaa
     9241 tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt ttaacgtgaa
     9301 ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat atctctggaa
     9361 aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat cgatcatttt
     9421 atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc gtacaccaca
     9481 aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg caaaaaattg
     9541 ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat aacccgtcag
     9601 ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg cttttgctac
     9661 atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg ccataatcag
     9721 tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa gagaagaaaa
     9781 agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca tccttttttc
     9841 cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat cgaatgctcc
     9901 taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg gactatctgt
     9961 acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct cggtcactat
    10021 ttcatttctt ggattcattt cgatcgttct atctagccaa tccgtattag ggagtaagcc
    10081 gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac ggcgatcgat
    10141 ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg catgtttgat
    10201 aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg cttttaattc
    10261 tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga ttgctgcttc
    10321 aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata aagggccgtc
    10381 acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg gtacattgat
    10441 agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg atataccgtt
    10501 agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct gttgattcat
    10561 cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca attttggacc
    10621 ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga cttgtccgcc
    10681 gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga tagctgcact
    10741 actagctgct ggtccaccgc caatcactaa tacatcataa ggttcttttt caaacaactc
    10801 ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca tgcgtccgct
    10861 actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt tatcatcgat
    10921 ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat ttaatacagc
    10981 taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc taacataact
    11041 gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt cactgatttt
    11101 cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag
    11161 ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag aaaaactagg
    11221 tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg ctatttcctt
    11281 cgtaaattca gctagttttt gactattttc actattatcc aaactaacag aaaaaacaat
    11341 aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt tgtctaacat
    11401 atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt cgccactttc
    11461 tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag cagcgcgaat
    11521 cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt ccatagcttg
    11581 aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt gctcttcatc
    11641 caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca cgtattcgat
    11701 tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg tatcggtaga
    11761 gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt gtaagtcttc
    11821 taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa tactccagtt
    11881 ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag catgtgcatt
    11941 aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa taattattta
    12001 tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg ttatttaata
    12061 aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag aaaatgctac
    12121 tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag catcatgtgt
    12181 aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa agccttgagt
    12241 aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt cactcgtaac
    12301 cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac ttcttttctt
    12361 cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg
    12421 tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac tattcgtgtt
    12481 tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg ttctcggaca
    12541 aagcgagcat tttctgcaga aacaagatta attttggaaa catacgtacc actttgaggt
    12601 atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg
    12661 ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg ttctaagtca
    12721 gcatagataa tttgtttacg aatcgcttga taagcttgtg attgcaagct gcttttcatc
    12781 tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct gaaaaacaat
    12841 ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt tgacatagcg
    12901 atttttattg cataaaattc acttgaatac aaaaactaat atgttagttt aggaggggaa
    12961 aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa aaagaagtag
    13021 aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga
    13081 tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct caacgtttag
    13141 cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat gaagaaataa
    13201 ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg ccaagcagtg
    13261 gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt catccagatt
    13321 gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta caattagtta
    13381 cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa tatataggac
    13441 cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg agtattcttg
    13501 ctgtgctact ttataaacgt tttttatctg gagcttggcc gattgcactt gtctctacag
    13561 ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc gcaaaagaat
    13621 ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat gaagaaattg
    13681 ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg tcagttagca
    13741 aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata
    13801 atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa gatcattttc
    13861 ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt gcaacggtag
    13921 ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg tctttagcta
    13981 ttatgggttg tttactgggg tataggagca tagttgagga gatgaaagat ccagatttat
    14041 cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag tctccaggta
    14101 tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct aatccaaata
    14161 ttcctaatat gcctcagcga atcgccacgg atacctctca aaaggtggcg attcgttatg
    14221 gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca cttgtattca
    14281 taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat caaggaaaac
    14341 cattcatccc gagtccagac ccactattga cagaccttca gcataagctt tccgatattc
    14401 aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt ttgtcaaata
    14461 aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata gaagaaatgt
    14521 tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac attacaggat tacttacaag
    14581 aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac aggaagaaaa
    14641 gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag gcacattgat
    14701 ggatatacca gtaggagagg tatggcctag ggagaggatc aaagaactaa aagaagaaat
    14761 tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa
    14821 aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa tccgaaactt
    14881 ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg actgggtgaa
    14941 atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta tatctgatga
    15001 tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca atgaatttgg
    15061 attgccggga tgggaacctg agcgcttagc tcctatcaaa tccttgcttg aagcctatgc
    15121 atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat caatcatccc
    15181 aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc catactcgat
    15241 ttttggatta gctcgaatcg taaagaatcg agaagattta gattggttgt gtcgcatagt
    15301 agacagccct tataacggaa tcactctttg tacagggagt attgcagaag atccagaaaa
    15361 tgatgtatat gggattttag cagaattttg ccaaagagat cgaatcccat ttgcacatgt
    15421 tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccacata aatccgaata
    15481 cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat ttaacggata
    15541 catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag gatacggctt
    15601 atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg
    15661 aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa ataagaagga
    15721 tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc
    15781 ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc
    15841 ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt
    15901 gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca
    15961 gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt
    16021 tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata
    16081 gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc
    16141 ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg
    16201 ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca atacgctatg
    16261 ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc
    16321 attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat
    16381 aaacaaatca aatacagtac aaagggactc caccatttct cttgaataaa gaacatgagg
    16441 aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt
    16501 cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag
    16561 attaggtcgc aaaatatcta cagagagatc aataaaaaag tcaatatatt catactctgt
    16621 gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa
    16681 tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa
    16741 aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt
    16801 acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc
    16861 agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact atgctaaaaa
    16921 tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact
    16981 gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac
    17041 tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat caattccttc
    17101 aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg
    17161 ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa
//
LOCUS       DAHEYX010000044        16841 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHEYX010000044 DAHEYX010000000
VERSION     DAHEYX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.044"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            9..974
                     /locus_tag="MB219_000988"
     CDS             9..974
                     /locus_tag="MB219_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCC8426718.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1073..2245)
                     /locus_tag="MB219_000989"
     CDS             complement(1073..2245)
                     /locus_tag="MB219_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426719.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2232..3377)
                     /locus_tag="MB219_000990"
     CDS             complement(2232..3377)
                     /locus_tag="MB219_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426720.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3391..4323)
                     /locus_tag="MB219_000991"
     CDS             complement(3391..4323)
                     /locus_tag="MB219_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426721.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4440..5519
                     /locus_tag="MB219_000992"
     CDS             4440..5519
                     /locus_tag="MB219_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCC8426722.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5516..6145
                     /locus_tag="MB219_000993"
     CDS             5516..6145
                     /locus_tag="MB219_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426723.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6255..6482)
                     /locus_tag="MB219_000994"
     CDS             complement(6255..6482)
                     /locus_tag="MB219_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCC8426724.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6585..9173)
                     /locus_tag="MB219_000995"
     CDS             complement(6585..9173)
                     /locus_tag="MB219_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCC8426725.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9193..9852)
                     /locus_tag="MB219_000996"
     CDS             complement(9193..9852)
                     /locus_tag="MB219_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCC8426726.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9901..10404)
                     /gene="trmL"
                     /locus_tag="MB219_000997"
     CDS             complement(9901..10404)
                     /gene="trmL"
                     /locus_tag="MB219_000997"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCC8426727.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10625..11491)
                     /locus_tag="MB219_000998"
     CDS             complement(10625..11491)
                     /locus_tag="MB219_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCC8426728.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11600..12241
                     /locus_tag="MB219_000999"
     CDS             11600..12241
                     /locus_tag="MB219_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCC8426729.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12303..13664)
                     /gene="mgtE"
                     /locus_tag="MB219_001000"
     CDS             complement(12303..13664)
                     /gene="mgtE"
                     /locus_tag="MB219_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCC8426730.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13682..14581)
                     /locus_tag="MB219_001001"
     CDS             complement(13682..14581)
                     /locus_tag="MB219_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCC8426731.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14583..15380)
                     /locus_tag="MB219_001002"
     CDS             complement(14583..15380)
                     /locus_tag="MB219_001002"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCC8426732.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15358..16062)
                     /locus_tag="MB219_001003"
     CDS             complement(15358..16062)
                     /locus_tag="MB219_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCC8426733.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16193..16768
                     /locus_tag="MB219_001004"
     CDS             16193..16768
                     /locus_tag="MB219_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCC8426734.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
BASE COUNT         4951 a         3658 c         2987 g         5245 t
ORIGIN      
        1 aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg
       61 gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac
      121 ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa
      181 cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca
      241 tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag
      301 aattcccagt tgcttatatt ccagcaggtt ctggaaatga ttttgctcga ggggcaggcc
      361 ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag
      421 tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
      481 tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc
      541 gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat
      601 tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa
      661 acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc
      721 caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct
      781 ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt
      841 tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag
      901 aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt
      961 tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg
     1021 gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct
     1081 ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg
     1141 aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg
     1201 tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg
     1261 gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc
     1321 caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt
     1381 tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag
     1441 tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact aacagaacca
     1501 ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt
     1561 tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc
     1621 gattaaacgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag
     1681 acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac
     1741 ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga
     1801 ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg
     1861 taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc gccatctttc
     1921 ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa
     1981 aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct
     2041 tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg
     2101 cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg
     2161 tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg
     2221 tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac
     2281 gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca
     2341 gtgtagaaat aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca
     2401 acaatcggaa tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca
     2461 aaaaagctca tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt
     2521 tttggtatca aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg
     2581 gtaacaatca tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag
     2641 acagctaaca taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg
     2701 attgaaacta acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga
     2761 tttgcttgct gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt
     2821 ccaatcagag tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct
     2881 tgaaaagata cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact
     2941 tctgccactt tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc
     3001 atcaacgtct gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg
     3061 agataaaaac cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt
     3121 tcctgtactt caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa
     3181 ttttccgaaa gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcaaga
     3241 ttgccacctt gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga
     3301 aaggccaatg tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta
     3361 taaaaatgcc aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc
     3421 taaaaagaca tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc
     3481 ttcttgtaag attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg
     3541 gagctgacct tttttataag cgattccatc agaagctatt gttcgctgaa tcgctgacaa
     3601 cacattttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc
     3661 ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga tcacgatctg
     3721 gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg ttgctccttg
     3781 tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt ctacagctac
     3841 tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa
     3901 ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt tttctaatcc
     3961 cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg aaacatataa
     4021 accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg caacgtcttg
     4081 aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg ttgtcccgaa
     4141 caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga tggcagtcga
     4201 tttcccactg ccgtttggac ctaacagacc taagacttct ccttcttgta cttgcagatc
     4261 aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa tttcgataat
     4321 catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag tgcaagaaaa
     4381 gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag gagtctgcta
     4441 tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca ttgcttttgc
     4501 tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc gttttattca
     4561 ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt attctatttc
     4621 ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg
     4681 cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg atttcgacgg
     4741 ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt
     4801 acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt gtctccaaag
     4861 atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg gccctgtcac
     4921 aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc cgagacatcc
     4981 atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg cttgaaatga
     5041 tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc gttcacactg
     5101 ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc tcttttcaac
     5161 actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa ggaaacttgt
     5221 ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa
     5281 ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga acgttgccgg
     5341 ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat tctgctcccg
     5401 gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt
     5461 tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga caccaatgat
     5521 aacagtagtc attattgatg atgatccatt tgtgacgaaa tcgttacaaa ccattttaga
     5581 aagcacgaaa gagatccgtg tgttaggcat tggtcattgt gccaaggatg cgctagtctt
     5641 atacgaaacc catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg
     5701 catcgatgct gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac
     5761 gacattcaaa gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt
     5821 aatcaaacag aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc
     5881 cgtatttggt aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat
     5941 tgatagtcat gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc
     6001 tgccgggaaa aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg
     6061 caattacatc agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat
     6121 ctatttttat cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc
     6181 cttcgcatca ttcaggtttt tttcacatct tctattcaat tgtcccgcaa accatttcct
     6241 cacgattgaa agaatcaagg attcgcaggg ctgccaggat tcgttggatt gttcggcgtg
     6301 ttaggcggaa aaataggcgc cttgtctaac gttttttcta gttttgattc ttcacgatcg
     6361 tctcgttcta catcaggact ttcattcaat ttgaattctt tttccatctc atctaacttt
     6421 ttaaagtcat tgccagattc ttttgctaat ttcgctaatt tatcttcggt ttctttagcc
     6481 ataaagtaac gccccccttt tgatttcagc ttagcatatc acactgaaaa aacaaaagaa
     6541 aacgctaacc aacgatcatt tttagctatt tctcatcttt aaaatcaagc attttttgcc
     6601 ggtataaaat cgtagggaga actgttttcc ataccaatca tcggatctac taactggtta
     6661 tcgatcactt ctgctgtaag aacgaaatct ttagaaacag caggttctac ctcttctttc
     6721 ttttcttctc caacaaatga agaaaacaga tctggttcca ctgtttccat tacttggttt
     6781 ttcgtttcat tcgtcccact gtcatcttct acaggtagct tttcttttac aacccgtggc
     6841 tcttttgtat agttcccatc ttcaaatgga tcatttccga ccaaagaatc ttcataagct
     6901 gctagtttgg tccggagagt gatattcatt ttatcattat ccatgatcct tgatttcaac
     6961 gtagtcatcc ggcttgctga ttcattcgct acacaagtga cataggcttg ctcttcccct
     7021 aacaaaatca acagttcctt actgcgagtc actgccgtgt agagcaaatt acgctgaagc
     7081 atccgctgat attgatggac catgggcaaa ataaccatct tgaattcact gccttgtgat
     7141 ttatggattg agcaacaata agaaagagtg attttattcc attcgtttct tttataagtg
     7201 acctcatttg catcaaactg gataactaat tcatccactt tatcttcaga atcttttgcc
     7261 aagatgatac ccgtgatttc tcccatatct ccattgaata cattcaattc gggagtattg
     7321 actaattgga ggactttgtc tcctatccga taaacggtgt cgttccaatg tacttccttt
     7381 tttccaggtt tccctggatt gaatatctcc tgcatcattt tatttaacgc atcgatccct
     7441 gcaggtcccc gatacattgg tgctaatact tggatatctt gtgcacggaa tcccttattt
     7501 ttcgcttttt ccacaatttt agagatcaag ggttcaattt gataagcatc acttcggaaa
     7561 taggaacgat ctttttgatt ctttgtgaaa tcttttggca gacgtccttc tttgatctca
     7621 tgggcaagag gaataatact agaaccatca ccttgccgat aaatctgatt gagttccatt
     7681 ttgggaattt gttcgatttc caaaagatca tggagtactt gaccaggacc gacagaggga
     7741 agctgatctt tgtctcctac taagataact tgcatattcg tcggaatcgc tttgaataat
     7801 gtgttagcca gccatgtatc gaccatagac atctcatcaa caatcaaaag tccgccttct
     7861 aattctttcg tactgactga aggtgttttt tcccgtccat tcagccccag aagacgatgg
     7921 atcgtactgg caggcagacc tgtcgtttcg ttcattcgtt tagctgctcg gccagttggt
     7981 gctgcaagta gaataggaaa aatttcttgg gtatattttg aaggatccaa ttccagtccg
     8041 ttcaactctg caaaaagatt gacgattccg ttgatcacag tagttttccc cgtacctggt
     8101 cctcctgtta aaataaatag aggggaacgg attgcttcag cgatagcttg ttcttgcgaa
     8161 ggtccatact gtatgccgaa tattttttcg atacgccgaa tattcttctg cacttctttt
     8221 tcaggataat ggatttcttt tttacgactg agaaggcgct ggatcgaatt accgatcccc
     8281 cattcagaaa agtacaaact gttttcgtaa agattagtct cttcttgttg tatctttcct
     8341 tcttcaacaa gttggataat tacagtagca acctgatcag gctggatttc aattgggcga
     8401 cttgtttcta aaatacggat cgtttgctga agcaatgtct ccgccggtac gtatgtatct
     8461 cctgtttgaa ctgcatgctg aaaaatctga tgcaagatag ctgctcgaat tcttctcggt
     8521 gaatctgctg caataccgat ttgttcggct agattatctg cacgtttgaa cccgattcct
     8581 tctatgtctt cgactagctg gtacggattt tcttcgatga tatccaaaga ctcgttctta
     8641 taggcctggt agatactgaa agctaactga ctaccgaacc cgtaacgatt cagtcccatg
     8701 atcacttgat ccatcccgtg gtttagccga atcgtctcag caagcatttc tctttttggc
     8761 ttcgtcaaac cggagatttg ttttaatacc gaaggatcag caatgatttt ttcgatagca
     8821 tcttctccca gcaattctac aattttttct gctgttttct tcccgattcc tgggaatttt
     8881 tcgctggaaa gatacgctac gatcccattt gcagttgttg gcttttcttg ctcatatctt
     8941 tcgagtttaa gctgccttcc gtatttagga tgatccacta attctccata aaaacgataa
     9001 gtctcatttt cttgaatatc gccgaaactt ccggtgacta caatctcttt ttcacggtaa
     9061 tcagctgtgg tttcactgat gtgtaccaaa agcactttgt aaaaattact aggattttga
     9121 aaaaaaatcg cggcaactgt accaacgaaa taaggcattt cttctggctg catcggaagc
     9181 cgcctccttc ttctatagta aaggttctgg tgctgtttca gttcctaaaa aatctgtttg
     9241 gttccatact ttcagttcat atggcaataa tctgcaaggc tcttcagtct ctaaaatcgt
     9301 caaactacta ttgaacaatc cacccatttc acgcaactca cttagctctt ttcctgtcat
     9361 ccattgaatg gctgcagtta aagaagctcc atgtcctacg aataaaagtg ggccatcttt
     9421 atgtcgggca accgcatctg ctacaacagt ttcgatacgt gtgattgccg attcaatcgt
     9481 ttctccgtga aaaggagaag gatcatacag atctaaatga ttacgcaaat tcggcagatt
     9541 atccggatat ttctggtaca tttcatcgat cgactgacct tctaaagtac ccagccctag
     9601 ttctcttagc ttatctgtat aaaaaatctc taccggacga gcaagctctt tgttgatacc
     9661 ttctgctgta tcacgtgctc gctttgctgt actggaataa attgctttaa aaggaacata
     9721 ttttaccttt ttcccaaatg ctttgatttc ttcgtaactt tgtggcaaaa gcggagaatc
     9781 gccattgctc ccttgaaaac gtctttcctg attccattgt gtcttcccat gtcgggtaaa
     9841 atatagttcc atacgcagca aactcctgtc tttcttttat tttactctat aataaactgt
     9901 ctaatccagc ttgtcatttt catagtgatg cgtcaattct aaattaggga atccttgctg
     9961 gcgaagtgct tcatacacta tcattgcagc tgtattcgat aaattcaatg agcggacatg
    10021 ctcatcattc atagggatgc gtaaacactt ttcttcgttt tcacgcataa atgtttctgg
    10081 caaaccggtt gtttcttttc caaataaaaa gaagtgctct tctccatcag caaaattttc
    10141 gtcactatat actttatggg caaattttga gatcaaatgt agtttacgat tacctaaaaa
    10201 tgttaaaaaa gcttctaaat tactgtggta agtaatattt acatcattcc aataatccaa
    10261 ccctgcccgt tttagatgtt tgtcatcagt tgaaaaacct agtggctcga ttaaatgaag
    10321 cggagtattc gtggctgcac acgtacgggc aatatttcca gtgtttgcag ggattaatgg
    10381 ttcaaataaa acgatatgat tcatcaggat cattcctctc atatttatgt tcgtttctat
    10441 tttctgacaa tttgattttt gcataaaaaa aaacgtatca actcggtgtc agtcactgcg
    10501 agtcgttacg ttagtgaagc tcttctcact aacttttatc agaacaggaa cctgataaaa
    10561 accaatgaaa aaacaacttg ttgctaatat aacaccctca actttttttt gcaacctcta
    10621 ctcattaaaa actgctagaa aaacgttttc tcttagttcc ttttaataat tctatgtcaa
    10681 ttgtaatctt cttcaaagga ttcttatata agtctgcttt gattcgctct tctacctgcc
    10741 attctaaagg tgacatggcc aaaagatctt tttgattttc ctctggtatc tcaaattcat
    10801 aggttacacg ctgacgttca gttaatttca ttgcttcagc aaacttggct actacacgtt
    10861 cattggaata actctgtttt tcaggttgat tcggataaaa agcctgacgt agttccttta
    10921 aataattttc ttgtggcacc accttcaaca cttctccatc ttctgagagt acacgttgga
    10981 attcttggta atgagaaggc gaaaagatat ttaaaaccgc gttgaatgtt tgatcagcaa
    11041 acggcaaatt ggtcaaatca gctacacacc aaaatgcttc ggcagattgt tctgtagctg
    11101 cgtagactcc ttcttttgaa atatcaaatc caacagcatt ttgaatcttt ttcaattttg
    11161 aaagttcatt caagaagcta ccttctccgc agccaacatc taatagcgag acttcttcag
    11221 gtaaatgctg cgcaatagtc tcaataacgg gctgatacat gccagattgg atcattctac
    11281 gccttggaac gaacattcct tgatcgtatt ctgttttaac cgcatgggat aaaaaataaa
    11341 gtgttccttt tttggaaagg tcaaaacggt gattattttt gcatatcaag ccattatcag
    11401 acttttttac ttcttcttgg caaaccggac agcggaacag ctgatgattt tctgccaaaa
    11461 actggcgtcc tcggtcaatt ttttttaaca tagttccccc tcatttctgt tttatcttac
    11521 cgttagatgc tcactttggc aactgctaaa aatatgatac aatgcctaga agaaagtgaa
    11581 agataaagga gaagattaga tgatcaaaga attttgcgct gaaaactaca catctattcc
    11641 tctagcaatt gctagaggag ccgatcggat cgaattgtgt gacaacttag cggtcggtgg
    11701 cacaaccccg agtactggtg tgatcgaaga aacgttaagt tatgctgggg aaaaagatat
    11761 ccccgtaatg acgattattc gtcctagagg gggcgacttc gtctataacg atatcgaatt
    11821 gaaaatcatg catactgatt tgattgaagc aaagaaatta gggacggatg gcgtagtatt
    11881 tggttgtctt actccttcag gctggctaga cgaagaggcc ttagaactat tgatcgaaac
    11941 agcagaaggg ttgcagatca ccttccatat ggcttttgac agtattcctg aagatcggca
    12001 attcgaagca atcgattggc tggtagaaca tggtgttgac cgtatcttga ctcatggcgg
    12061 tcctgctgac aaaactattg tagaaaattt cccacggctt aaagaattga ttgcctatac
    12121 agatggccgc ttaatcattt tgcctggtgg tggtgtcact gcagagaatg cagcacatgt
    12181 agctaaagaa ttgaacgtaa gcgaagtaca cggaaccaaa atcgtggatc ttcagttata
    12241 atcttataaa caaaaaaaca tttgcaacag gcataatgct tattgcaaat gtttttgttt
    12301 ttttacataa aatagcccat aaataaagaa gcaatattga aataaatcag tacactcgtc
    12361 aaatcgctca aagtcgtgat aaagggaccg cttgctactg ctggatcaaa tcctagacga
    12421 tccattagca tcgggattaa actacctgca agattcgcca ctgtgattgc aaatagcata
    12481 gccatcccta tgacaaaccc caagataaaa ttatgtttcc aaaaacctac gattagaaaa
    12541 atcgtgatcc ccgtaacagc tcctgtgaca acgccggtca agacttcagc caaaacaagt
    12601 cgaacaaatc catgatcttt ctcatcagaa atcgcaagtc gacgaacagc cacagctagc
    12661 gactgggttc cagcattacc ggcagttcct gtaatcagcg aaatgaagac tgctaaaata
    12721 ctcgcttcac ttaccaagtc ttcatagtga ctgattaaag aagcggtcga catacctaaa
    12781 aacaacagag tgatcagcca aggcaaacgt cgagacgctg cctttactgg attttcatta
    12841 atttcttcca cattgacccc ggcaagtcct gaataatcgc tagcagcttc atcatcaata
    12901 acgtcgatga tatcatcgac tgtgacgatt cctaacaaat gatcatcata atcggtaaca
    12961 ggaagagcta agaagtcata atctctaaat gtttgtgcca catcttcttg gtcgtcgcct
    13021 acatgaactg aaagcactcg ttcgctcatc agatcagaaa tcattgcgtc gtcatcattc
    13081 acgattaggt cgcgcagaga aataacccct actagctgac cttcttgatt aacgacatag
    13141 atgtaataga ttgtttcagc tacatctgct tcgttcttca gcacgtacat cgcagagcga
    13201 acagtttgat tggctacgat cgaaacaaat tcagttgtca tgatcgcgcc agccgtttca
    13261 tcctcgtaat ggagaagttc cttgatttca ctggcatcgt ctgccgacat caaacttaaa
    13321 tatttagcaa tttgcttttt atccagcgta ttaagcaagt caacagcatt atctgtatac
    13381 atctcagaaa gcatatcagc tgcatagcta ggacgcattt catctaggta ttctttcata
    13441 tgttcgttgt cttcttcaat cacatcgaac atatccgcta attctttagg cgacagatag
    13501 gaatagatca gctgcctgtt ctcttctgta agagattggt agaattgccc ctgctcatag
    13561 atatgcatct ctaaaaaact gtttctgaat gcctgaatat tattttccct taaatcttga
    13621 gtcaactggg caaatctttc ctctaattct tgattttcat ccaaaccaat ctcacctcgc
    13681 cttattcttc aaaccgtctc acaactgcct ccatgtctgc ggctaaaggc tgcttgaact
    13741 ccagcaactc ttctgtaaat ggatggtaaa aacgcagatc ataacaatga agggcttgtc
    13801 tttttattcc cagatccatt cgcccgccat acatatcatc accgagcagg gcacagccaa
    13861 ttgcggaaaa atgaactctt atttgatgtg tccttcctgt atgcagctgg atatcaacta
    13921 acgcgatttc tgttagtcgc ttctctatcc agtactcagt ctgtgccgaa cgtccttcat
    13981 cagagaccat tcgcttcaat agcgaagaat aatctcgggc gatcggcaaa tcgatatgtc
    14041 catgtgccga taaatctgaa agccttccac tgactaaggc ctgatatttt ttcacaaact
    14101 tcttttcacg aagttgcata tctaattttg cgtgcgcaaa tccatgcttt gcaaaaagca
    14161 tcaagccaga tgtatcccga tctagtcgcg tcaccacatg gattacttgg ttttcgtaac
    14221 cttgtttttt ataataagcc tttacacgat tagccattgt cccatttgga tggtattggg
    14281 caggaataga cgaaacgcct gctggtttat ttactaccag aacgtgttca tcttcataca
    14341 cgatatcgat cggtgtttca tccaaaagta cggtttcatg ttctccttca gcaggaatca
    14401 caatggttac cttatcatcc ttagctagag aaaacaagac attttctact tgatcattaa
    14461 ctttgatttg accgccttga aattttattt tcgctaacag accctttgag atcccttttt
    14521 cttttaaaaa atattttact tgctgagaat gatctttctc ataatcccat gtaaactgca
    14581 tatcaatcct cactaataaa ggcatctttc acacgatgcc aaaaatgcat atgtcgataa
    14641 gaagcaaaat ggatccgttc ttctgctatt cgataataaa ttgagcggat atttgctttt
    14701 gcgacattca attgatcaac cgttacaaga taatcttctg tattttccaa ttttatttct
    14761 agccattcat catgggcaat cacgatcgga gaacccaaag tacgaaacac tcgattattc
    14821 agtgaagcaa tctctgccaa ctgaaaggca ttgatactag gatggatcac agcaccgccg
    14881 acacttttat tatatgctgt cgaaccagta ggcgtagaaa tcgtcaatcc atctcctcta
    14941 aaactttcaa acagctcgtt tttgatgtat acatcggcta ccatcgtacg attggcccgt
    15001 ttaatcgttg attcattcag tgctaaaaaa tgctggtccg gtgtttcatc caaataactg
    15061 atccgaacat ccagcaaggg ataactaaca ctttgttccc ggttcgttcg taagctgtct
    15121 actagttctt ccagctcgta atcccgccaa tccgtgtaaa accctaaatg acctgtatgc
    15181 acgcccaaga aacgcacatc attcagacaa tgactgtagt gatggaacgc cgacaataat
    15241 gtgccgtctc cgccgactga gatgaccaat tctggctcat gatcatctcg ttgaatccct
    15301 gcttgttcta aaagtgcgtt caaacgttct gtcacttcta tagtctttgc ttctttatta
    15361 tgaacgatcg ctactttcat cccctgcctc ctctctttcg ctttctttct tcaaaagcgt
    15421 tcgatagtag acatcatctg attttcctct tccatgggag aataaccgct gtgcttcttg
    15481 gatttcttca cggatactgg acatctcttc atccaactga taggcagctt ctgccgcacg
    15541 ctgaagtcgt tcactcattt cttctggaaa aacgccttga tatttataat tcagcgaatg
    15601 ctcaatcgtt gcccagaaat tcatcgcaag agtccgaatc tggatttccg ccaaaatttt
    15661 cttctcaccg ctgatcagct gtacaggata ctcgatcacc agatgataag aacggtagcc
    15721 acttgctttt ttatttgtga tgtaatctcg ttcttggaga actttcatgt cttttctttt
    15781 acggatgatc tcaacaactt gatggatatc ttctacaaac tgacacatga tccttaaccc
    15841 ggcaatatca gacatttctt cttctaaacg tcccattgga atatgccgta attgtgattt
    15901 tgttacaata ctatcgactg gcttgactct cccagtgaca aattcaatcg ggacatgtcg
    15961 attttgttca cggaactgtt tgcggatccc ccgcagtttc actttcaatt ctgatacagt
    16021 ttgttcatat ggtgctaaaa atgcttccca atctcgttcc atagctgata cctctacttc
    16081 attaacattc attgtttatt ctaccatata tgcaaagttt tttcgtactc ttgatttatg
    16141 cattgaccaa aaaaagaaaa ttaggcaaaa tagacaaaaa ggagtcgatc aaatgacaca
    16201 aatgcttgaa atcgaatata aatctatgct gacaaagcaa gagtacgaga tattcatcgc
    16261 tcactatcaa ttaacagata aagatttccg tgtacagact aatatttatt tcgataccgt
    16321 tgatgaacag ctgaagaaac aaaattgtgg cctacggatc cgtttcgttg gtcatcaagc
    16381 tgaatataca ttaaaaacac ctgctgaaaa agggcgtctc gaaacaacgg atacctttat
    16441 aacagaagaa gcagaagcat ttatcttcga aaaacgtctg cctcgttcag gcgcagtttt
    16501 tcaaaaactt tctgatttga atatcgatcc tgcttcatta atccaaaccg gaaagcttac
    16561 aacaaaacga gctgaattcc ctattgaaga aggactttta gctattgatg aaagctgggg
    16621 agacaatcta cacgattttg aactggaact agaggtaggg gatgcatctg tgggaaaaat
    16681 tgcttttata aattttttaa aacgtttttc tttaccttat cgtcctgcta agaacaagat
    16741 ccaacgtatg cttgaagcta aaaattagaa acctttcatt ttcacaaggc acttgagttc
    16801 atttttttac ttttttctgt taagttatag gatgtgaaag a
//
LOCUS       DAHEYX010000045        16585 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHEYX010000045 DAHEYX010000000
VERSION     DAHEYX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.045"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1474)
                     /locus_tag="MB219_001005"
     CDS             complement(<1..1474)
                     /locus_tag="MB219_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCC8426735.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRI"
     gene            complement(1471..2157)
                     /locus_tag="MB219_001006"
     CDS             complement(1471..2157)
                     /locus_tag="MB219_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8426736.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2350..3771)
                     /gene="gndA"
                     /locus_tag="MB219_001007"
     CDS             complement(2350..3771)
                     /gene="gndA"
                     /locus_tag="MB219_001007"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCC8426737.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3941..4120)
                     /gene="rpmF"
                     /locus_tag="MB219_001008"
     CDS             complement(3941..4120)
                     /gene="rpmF"
                     /locus_tag="MB219_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCC8426738.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4178..4747)
                     /locus_tag="MB219_001009"
     CDS             complement(4178..4747)
                     /locus_tag="MB219_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCC8426739.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4933..5835
                     /locus_tag="MB219_001010"
     CDS             4933..5835
                     /locus_tag="MB219_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCC8426740.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5870..7654)
                     /gene="pyk"
                     /locus_tag="MB219_001011"
     CDS             complement(5870..7654)
                     /gene="pyk"
                     /locus_tag="MB219_001011"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCC8426741.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7728..8690)
                     /gene="pfkA"
                     /locus_tag="MB219_001012"
     CDS             complement(7728..8690)
                     /gene="pfkA"
                     /locus_tag="MB219_001012"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCC8426742.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8858..12172)
                     /gene="dnaE"
                     /locus_tag="MB219_001013"
     CDS             complement(8858..12172)
                     /gene="dnaE"
                     /locus_tag="MB219_001013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCC8426743.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITNYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12399..12584
                     /locus_tag="MB219_001014"
     CDS             12399..12584
                     /locus_tag="MB219_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCC8426744.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12646..13842)
                     /locus_tag="MB219_001015"
     CDS             complement(12646..13842)
                     /locus_tag="MB219_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCC8426745.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSESTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFILSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14012..15448)
                     /locus_tag="MB219_001016"
     CDS             complement(14012..15448)
                     /locus_tag="MB219_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCC8426746.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            15741..16550
                     /locus_tag="MB219_001017"
     CDS             15741..16550
                     /locus_tag="MB219_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8426747.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4862 a         3500 c         2915 g         5308 t
ORIGIN      
        1 agattctaaa aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt
       61 tttcaaccag ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc
      121 ttgctttatc cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc
      181 cttcgccaaa gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat
      241 ggacttcttt tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt
      301 gttcaaagtg attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag
      361 tgatcacata gtctggatat aatatcttga aattattata aacttgatag gtgacttctt
      421 ttcctttagt tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta
      481 gcatctcttg aactaagctt ttcatacggc taatttcttg catgctggcg cttaatgatt
      541 cttctaatat ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa
      601 taattgcaac aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt
      661 caatatacaa acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt
      721 catcatttgt gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat
      781 ccattgtatc tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta
      841 aaatactact taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat
      901 ttcgaatatc atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc
      961 ttgtaccttt agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa
     1021 cgatcgttgg aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa
     1081 aaatcaagtt ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag
     1141 ctataaagga atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct
     1201 catctccgtc atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt
     1261 catccgagtt agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt
     1321 cttttgccac aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca
     1381 caacgaatat gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca
     1441 gttctctttt ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg
     1501 tacagtttgg atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat
     1561 atatacgtcc actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa
     1621 aacgtcacgt gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc
     1681 tctttttgtt agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat
     1741 agtcaaatcg cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc
     1801 gattcgacgc aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac
     1861 gatgtagtca tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc
     1921 agtcatcata atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc
     1981 gttcaattct gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc
     2041 atctaaacca gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc
     2101 tagctcgaca aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta
     2161 ttattttatc ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat
     2221 gcttaaaaat ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta
     2281 ctcataacaa tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc
     2341 gaaagactat tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg
     2401 ttcataggta tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg
     2461 ttctgctctg tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc
     2521 agcttggaca gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc
     2581 aacgaagtaa tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt
     2641 aattttttgc aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc
     2701 accaaatggc aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg
     2761 ggcataactc atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt
     2821 tttgtctccc gtgaactcaa aatcgttcgt acgagataaa atcttgcttg cttgtacgcg
     2881 ttcttcttta taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg
     2941 cacacctaag tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc
     3001 atcaaggatc acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat
     3061 gtcagcagtg atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat
     3121 atcagccatt tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc
     3181 aatcagttgc atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc
     3241 tccatttggg ccaatatatg tgacacatgg tgcttcgtct tctgcttttg cagaaatttg
     3301 ctctaagatt ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg
     3361 tccttttaaa gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga
     3421 gttggctaac tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat
     3481 caaaatatcg cctttatcaa ggtgaggaag caactcttgg atcgttgcat ctgttgccgg
     3541 tcctgctttg accattagca agatccgacg tggtttctcg atagattcta caaattcttc
     3601 gattgtatat gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt
     3661 agcccctgta cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag
     3721 atttttaccc ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc
     3781 tcctacatac gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca
     3841 aactaaagaa caacatattt tttatttacc gactacaaaa aaataaacct caaggctcca
     3901 tggttagcct tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta
     3961 cgtccttacc atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac
     4021 cacagtttgg acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg
     4081 cttttttagc ttttgatgtt cttctagctg gtactgccat tttgctacac ctccttgtaa
     4141 agtcttgtat ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg
     4201 atttagaatc aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt
     4261 cttccatttt tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg
     4321 gaagttctgt tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga
     4381 tgttgtcttc aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat
     4441 ccgcagacat ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta
     4501 cagaaaaatc catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag
     4561 tcgtctgcac agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga
     4621 cttccactgg cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt
     4681 cgagcgtctc atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc
     4741 atttcatttt tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg
     4801 tcaatgaaat tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa
     4861 agcctctgtt tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag
     4921 cggatgatct tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa
     4981 aattttacga aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta
     5041 caacgtcttg aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa
     5101 ttaatcgtca cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag
     5161 atttctactg caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg
     5221 cctcctatta tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa
     5281 ttgatgaatc aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga
     5341 caagggagga tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta
     5401 gaaacagagt tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt
     5461 tccaagcagg atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac
     5521 gaacattatg tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca
     5581 aaagttgttt atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg
     5641 ggtatcggtt ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct
     5701 ctttcagatg aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc
     5761 gtcacgcctc ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa
     5821 aacagctctt cgtaaaagaa gagctgtttt tattttttat taagctaaat tagatagctg
     5881 ttgtttcgcc acggtaaacg atgccgcgac gtgaatcaac ggtaatcaat tcaccttctg
     5941 taattgttgt tgttgcgcct ttagcaccaa cgattacagg aatatcttgt gcaattccta
     6001 caactgcagc gtgagaagtc aatccgcctt cttcaacgat aacagcagct gctttttcga
     6061 ttgcaggcat atattctttg tcagttgatg gtacaactag aaccattccg tcttttgctt
     6121 tttttacagc ttcttcagca gatgaagcaa taactgcatt tgcaacgaca gatttttgtc
     6181 caacgccttg agcttctaat aatttagaac caatcatttg aattttcatc acgttagttg
     6241 ttccgcgttc accaactggt acaccagcag tgatcaagat taaatcgcct tctgatgcga
     6301 aacctaattc aacagctttt ttagctgcta gttcaaacat ttcatcagtt gttgatggtt
     6361 tttcagcaac tgttgggtaa acgccccagt ttaacatcaa accacgtttt gtgcgttcgt
     6421 caaaagtaac agccaagata tctgcatctg gacgatattt tgaaatcatt tttgcagtgt
     6481 atcctgattc agttgcagca acgattgttt tcacgcctag attttttgca gcacgagcta
     6541 cagataaacc gattgtttca gttacatctg ttttatcgaa ttcgttcaat tggaatgtac
     6601 ctaattctga taatgcattt tcagcttctt ggtcgatacg tgccattgtt gaaacagctt
     6661 caactgggta gtccccgttt gcagattcac cagatagcat tgttgcatct gtgccatcaa
     6721 atacagcgtt agcaacgtca gaagcttccg cacgtgttgg acgtgggttt tcttgcattg
     6781 attctaacat ttgtgtagca gtgataactg ggataccagc agcattacat tttttgatga
     6841 tgcgtttttg aaccattggt actagttcag ctgggatttc aacacccata tctccgcggg
     6901 caatcataac accgtcagaa actttgatga tttcgtcaat gttgtcgata ccttcttgag
     6961 attcgatttt agggaagatt tgaacatgag tcatgtcttt ttcttctaag atttcgcgga
     7021 tatctagaac gtcttgtgct ttacgtacaa aacttgcagc gataaagtcg atatcgttgt
     7081 ctaaaccaaa acgaatatcg tcagcatctt ttggagtaat acctggcaaa ctaatagaaa
     7141 cgcctggtgc atttacccct ttacgtgatc ctagcatacc tgcattttta acttctacga
     7201 ctaattcacg gtttgcttcg tctttttcaa tgatttgcat atcgatcaaa ccatcatcaa
     7261 ataaaatgtg tccgccaacg tgaacatcgt caaataatcc tgggtaagta accgcgattt
     7321 tttcttttga acctacatgt tcaggatcca tagagatgcg tgtttgatct cctacttcaa
     7381 atttgatgta tcctgcacga ccaaaatcag cttctgttgt gccttgaaca gttgtacgga
     7441 tttcagctcc ttttgtatct aaaagaatac cgacatcttt tcctgtcttt ttaactgctt
     7501 cacggaccat agtcatacgt gataattgtt cttcatggtc accatgagaa aagttaaagc
     7561 ggaagacatt tgccccagat tcaatcaatt gagaaataat ttctactgag ttacttgctg
     7621 gtcctaatgt actaacgatt ttggtttttt tcatttaaaa aactctccca ttcaattttg
     7681 tttatttaaa gctgtacaca gcttaaatag cttggtttct ttcactctta aaatgaaagt
     7741 gaattattta gatcatatag tgataagtct ggtttatgtt tgttgttttc aagtgtatca
     7801 atgatgtcag acgcaacgat ttcgttgtca tggataccga cacataatcc accttttcct
     7861 tcttgaagca attcgacagc gtaagcaccg aatttacttg ccaacacgcg gtcgcgtgcg
     7921 cttggtgctc caccacggac aacgtgtccc aagatagata cacgtgtatg atagtcgcca
     7981 aattctgaaa gtttctcagc gaattcattt ccgcccatta caccttcagc caagatgatc
     8041 aagcaatgtt tcttcccgcg atcgcggcct tcttggattt ttttagcaac tgaagccata
     8101 tcaaaatcat gttctgggat gatgatttca tccgctccgc cagccacacc tgaccataaa
     8161 gcgatgtctc ctgcattgcg tcccattact tcgataacga atgtacgaac atgagaagtc
     8221 gctgtgtcac gaatacggtc aattgattct agtactgtat tgattgctgt atcaaaaccg
     8281 attgtgaaat cagtcccagg aatatcatta tcgattgttc caggaatacc tactgctggg
     8341 aaaccgcgtt ttgtcaaagc cattgcgcca tgataagagc cgtctcctcc gataaccact
     8401 agaccttcga ttccgaattt tttcaattgt tcgattcctt ttaattgacc ttcttcagtt
     8461 gcaaattctg gataacgtgc agaatacaag aatgtaccac cacgttggat cttgtcacct
     8521 acatctgcca catcaagacg acgaatatct ccagcaacta aaccggcaaa accataattg
     8581 atcccgtata cttccagtcc ttcgtaaata cctttacgaa caacggcacg aacagctgca
     8641 ttcattccag gtgcgtctcc gccactggtc aaaattccga tgcgtttcat aataaacatt
     8701 cacctcatta aaatacttta tcctaagtat gacttaggtt tacaaccatt tttgcgttaa
     8761 ccacgactaa cattctaaca ttatttttct cacttgtctt gatttacgga taaaaaaatg
     8821 aaaaaaaatg aaagcaagta gtatttttta ccgtaagtta tttgaaaacc acattatctt
     8881 cgccaagcag ataaataagt tgccccttta tactcttgct gtcatccacc caataagcat
     8941 ctgacagaac tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg
     9001 ggtgtttctg aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg
     9061 taatacgcaa atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa
     9121 agctttctcc aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa
     9181 caaagatcac tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa
     9241 ctgtgacaga aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt
     9301 tttttgtccg aatttctcta acttccttga tcatcactaa taaattgact ggttgattag
     9361 ggacgatatc tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg
     9421 caggatgacc tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta
     9481 gtgtatagtc agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc
     9541 caccactgta aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt
     9601 taggagataa ttcatcaaaa acgcctactg ctgtcagtga ttgcagtaac tctaatttca
     9661 gccaacgctt atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt
     9721 ttctttcgtt taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc
     9781 cgaatctgat ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg
     9841 gcgaggaaag tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg
     9901 tgttatgccg aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta
     9961 gatacgccat ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat
    10021 agtttgcaaa tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct
    10081 gttgtaatgc tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt
    10141 tactcactgc ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg
    10201 cgatctgcat gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga
    10261 tcagtgaatc atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat
    10321 ctatattttg catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt
    10381 tttctggacc taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc
    10441 ctgaggtttc gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt
    10501 ttaatcggat ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg
    10561 ataagttgcg tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca
    10621 tcgtatactg ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta
    10681 gattcttatc gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt
    10741 ctaaaatcac tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta
    10801 ctaattgatg cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg
    10861 cttttgacca gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa
    10921 gaaccatttt tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat
    10981 acttttctcg aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga
    11041 tatctggcat cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta
    11101 atggatccac atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac
    11161 gacctgcacc ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca
    11221 agaaataatc atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt
    11281 ggtattcctt tgtataatta gtgattcggc gttccacttg cgaatagcat aactctctta
    11341 aatattctcc agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga
    11401 actggaattc aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa
    11461 gacccgcttt ttcgaatcgt ttgatcattt cttgttctga aagaagatag ccatttgctg
    11521 ttagtggtaa agatgcagat aaattttcta aaggtatttg gcttccatct ttgatatgtt
    11581 tcatcacttc aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac
    11641 aaagcgaata attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt
    11701 tttctatcaa gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat
    11761 gaccgaaaaa gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa
    11821 caaacgctag atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt
    11881 ttcggctgga aagttttatc agctgctgat aacctatttc atcttttgcg tacgcaagca
    11941 agccataatt tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc
    12001 cttcttctac acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta
    12061 tggctaatga agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg
    12121 tactttgtaa aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt
    12181 ccgccttttt tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt
    12241 aagatagcag gcgctgaagt tgattattcc acttcatttg cttgtaatct gtaggaatga
    12301 gttctttcgc aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga
    12361 aaatctggta aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat
    12421 tctgggctgt cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa
    12481 catatgattt tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga
    12541 ttggtgcagt tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc
    12601 ttgagatatc tcaagctctt ttttattttc tgctcctcac caccattata atctgctgcg
    12661 aagaacttta cgaaagttga ataaagaaag aaaaatattt gacagaataa acaaacttgt
    12721 cacaataaaa acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc
    12781 taaaacttgt ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc
    12841 ttgaggcgta atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa
    12901 gcctaaaata aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa
    12961 ataacagcaa aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc
    13021 ccctagtttt cctagtttcg gtgaagaaaa aacagcggaa acaccagcta tcgaaacaat
    13081 caagccgctg acaaaaagga tattttctgt actttcgctc aattcacgaa tatataaggt
    13141 caaaataggg ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc
    13201 gatcaaaata gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt
    13261 tttctcgatc ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat
    13321 caacaaagcc cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc
    13381 gcccattgaa ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc
    13441 ccatccgctc ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata
    13501 tccagacaaa attccattcc aaaaccgcat gatcaaaagc caatacgcgt tcggtacaaa
    13561 agccaaagat cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg
    13621 tccttttcga tcagctagat tcccccagat tggcgcaacc acggcagatg caaaagcagt
    13681 cacagagatt gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc
    13741 aacataaact ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc
    13801 aatccacgca atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt
    13861 ctcttttatc atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa
    13921 aatcccgcaa aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag
    13981 aacaaatact gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga
    14041 taatgaataa aatatcaacg atccacaaag caacagaaac ttcattgtat tttcctttga
    14101 ttactttcac aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc
    14161 acaatcccat aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt
    14221 tgatgtctgc aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg
    14281 cttgatttgg tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg
    14341 ccactactac tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga
    14401 cataagttgt tgtattcgat gtaccaaaga tagcccccac ggaagtagcg atcgcatcgg
    14461 caaatagtgc tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt
    14521 cttcttttga gaaaatgcct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga
    14581 aagtatcaga taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat
    14641 cactgaataa agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt
    14701 ctttaaatga attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac
    14761 ccataaaaat accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat
    14821 tttttacaac taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt
    14881 tgtcaaagtt ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc
    14941 cgccttcaac gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg
    15001 catttttgat tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa
    15061 gactttcggg aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat
    15121 tgattaatcc gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa
    15181 aaacaaccgt aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta
    15241 catttgcgaa caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa
    15301 atactgcttg gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat
    15361 aactcatggc aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt
    15421 ttttctttaa accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat
    15481 ggaaaactat aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat
    15541 tatatagacg tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact
    15601 taacgttcgt gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta
    15661 ggaatagctg attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt
    15721 tgaggaagga aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta
    15781 ccctcaatca agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca
    15841 atgccggtca tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt
    15901 accaacaatt agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc
    15961 ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa
    16021 tcttatcgca aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt
    16081 tttttattga cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag
    16141 agaagaattt gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa
    16201 cagacaaacg ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg
    16261 tcgatgttcg gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc
    16321 aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca
    16381 ttgcctttgg tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag
    16441 ctatggctaa cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca
    16501 atcaggaaga cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta
    16561 gattccacaa aaaagaggtt gtgac
//
LOCUS       DAHEYX010000046        16463 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHEYX010000046 DAHEYX010000000
VERSION     DAHEYX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.046"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            1..1140
                     /locus_tag="MB219_001018"
     CDS             1..1140
                     /locus_tag="MB219_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCC8426748.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1133..1933
                     /locus_tag="MB219_001019"
     CDS             1133..1933
                     /locus_tag="MB219_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCC8426749.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1933..2760
                     /locus_tag="MB219_001020"
     CDS             1933..2760
                     /locus_tag="MB219_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCC8426750.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2738..3472
                     /locus_tag="MB219_001021"
     CDS             2738..3472
                     /locus_tag="MB219_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCC8426751.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3572..4438
                     /gene="rfbA"
                     /locus_tag="MB219_001022"
     CDS             3572..4438
                     /gene="rfbA"
                     /locus_tag="MB219_001022"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCC8426752.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4452..5024
                     /gene="rfbC"
                     /locus_tag="MB219_001023"
     CDS             4452..5024
                     /gene="rfbC"
                     /locus_tag="MB219_001023"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCC8426753.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5046..6074
                     /gene="rfbB"
                     /locus_tag="MB219_001024"
     CDS             5046..6074
                     /gene="rfbB"
                     /locus_tag="MB219_001024"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCC8426754.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6172..7023
                     /gene="rfbD"
                     /locus_tag="MB219_001025"
     CDS             6172..7023
                     /gene="rfbD"
                     /locus_tag="MB219_001025"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCC8426755.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7058..9091
                     /locus_tag="MB219_001026"
     CDS             7058..9091
                     /locus_tag="MB219_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426756.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9135..10415
                     /locus_tag="MB219_001027"
     CDS             9135..10415
                     /locus_tag="MB219_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCC8426757.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10625..11431
                     /locus_tag="MB219_001028"
     CDS             10625..11431
                     /locus_tag="MB219_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426758.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11443..12663
                     /locus_tag="MB219_001029"
     CDS             11443..12663
                     /locus_tag="MB219_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8426759.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12653..14239
                     /locus_tag="MB219_001030"
     CDS             12653..14239
                     /locus_tag="MB219_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCC8426760.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14278..16416
                     /locus_tag="MB219_001031"
     CDS             14278..16416
                     /locus_tag="MB219_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCC8426761.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5218 a         2726 c         3501 g         5018 t
ORIGIN      
        1 atgcttgttt cttttagtat ggttataaga ttttttctga caattttatt atcactgata
       61 ttaacacctg tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa
      121 agaagaataa ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt
      181 gttatatcga ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc
      241 ttgattgctt ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca
      301 cctaaacaaa aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat
      361 atacgtattg attccttcac gcttcctttt attggatata tccaactagg ctggcttagt
      421 tttcccgtaa cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt
      481 ttggatgggc tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga
      541 tattttttcc tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca
      601 agtattatcg gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca
      661 ggagctctat tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg
      721 acttttgttt catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt
      781 tttgctatca ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat
      841 cttcatcacc gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat
      901 gcgatggctt tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga
      961 acgatcatat tgattattgc catgttgttt gctgcaattt tattatttga attgattggt
     1021 ctgatcaatg agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag
     1081 taccgtacgc aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa
     1141 caaattaaac cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg
     1201 gacactagat aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt
     1261 tgacaacaat tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta
     1321 catcgatatc tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct
     1381 gatagctagc gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga
     1441 gacagttaca gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc
     1501 taagattctt aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt
     1561 tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact
     1621 ggctgtgttt gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc
     1681 aggtagtttt atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg
     1741 tttcttcatg tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa
     1801 gttattgtac acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa
     1861 tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg
     1921 gcgttttttc tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc
     1981 agtcaatctc cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt
     2041 attatttacg ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc
     2101 gtttatcatc atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct
     2161 caagcagatg aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac
     2221 gattattttg aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg
     2281 atgatttttt ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt
     2341 acagctcaag cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca
     2401 ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct
     2461 ttagattttt tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag
     2521 atccttcctg tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt
     2581 aatcgatacc gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg
     2641 catttatcca gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc
     2701 aaaaaccggc atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga
     2761 attcgtgcgt tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt
     2821 agatagtatt ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc
     2881 agtagatgat acatgggata tattaactga atacgcagaa aaagataagc gaatccagct
     2941 tttttcgggg cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa
     3001 aggagatcta atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa
     3061 gatcactgac tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat
     3121 tgtggacagc gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga
     3181 aggatggctt aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc
     3241 ttctttaaaa cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat
     3301 tggtttgctg ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag
     3361 acatgattta aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg
     3421 gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat
     3481 gtttaaatcc ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga
     3541 aatttgaaat caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag
     3601 cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga
     3661 caaaccaatg atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct
     3721 gattatttca acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga
     3781 tttaggactg cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt
     3841 tatcattgga gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat
     3901 ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc
     3961 gacagtattt ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga
     4021 gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt
     4081 aactggcctt tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc
     4141 agaacgtgga gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact
     4201 atccgtagaa cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt
     4261 gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg
     4321 tttggaagaa attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc
     4381 acagccattg aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg
     4441 agtccttaca gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg
     4501 acgtctttgg cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag
     4561 aacatggatt gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg
     4621 tccttcgggg gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag
     4681 tcactggagc agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc
     4741 aatgggacgg atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat
     4801 atgcccatgg attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt
     4861 actataatgc agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt
     4921 ggccaattga tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa
     4981 aagaatttga agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt
     5041 ttcttatgaa aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc
     5101 attacgtggt aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg
     5161 caggaaacaa agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag
     5221 atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt
     5281 acgcggcaga atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga
     5341 atatcattgg tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc
     5401 acgtatctac tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg
     5461 gggaaggcga aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt
     5521 cttctacaaa agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac
     5581 gtgcaaccat ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta
     5641 ttcctcgcca agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa
     5701 agaatgttcg tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga
     5761 cgaaaggcag aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga
     5821 ccgtgatgga attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa
     5881 acgaccgcgc agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc
     5941 taggttggca gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt
     6001 acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca
     6061 aaaatggaca ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca
     6121 ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta
     6181 ttaactggag gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat
     6241 ctgccatatg tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg
     6301 gcttatatta cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta
     6361 gataaagcag aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga
     6421 aatgtagcac ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat
     6481 gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta
     6541 aatgaatatg gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac
     6601 tattatatca tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg
     6661 atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga
     6721 ccaacttgga cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca
     6781 tttggtattt atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa
     6841 atattgaaag atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa
     6901 gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc
     6961 ataccaactt ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag
     7021 taatttcctt tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc
     7081 acgacgaaaa gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt
     7141 tctaaaactc cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt
     7201 aaacggaagt tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa
     7261 aactgatgtg atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac
     7321 acctttttac ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt
     7381 aaacggacaa aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg
     7441 aaaaccattc aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg
     7501 ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa
     7561 gaggaaaaaa gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca
     7621 gtggtggttc atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc
     7681 tttttatcat tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt
     7741 ggtggttatt ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct
     7801 ggcgtacttg ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc
     7861 atgggatctg ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat
     7921 ggttcatttc tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc
     7981 aggaaaacgg gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac
     8041 aaattggaaa attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt
     8101 ctatcacttt tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga
     8161 tagcgaacaa aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg
     8221 gattaccgtt gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc
     8281 attaagagcc atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc
     8341 ttatatttgg agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa
     8401 tgcatggtct tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt
     8461 tgaaaccatc gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg
     8521 gagacgcttg ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa
     8581 tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa
     8641 aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac
     8701 cccaacattg ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg
     8761 gaagaaaatc gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag
     8821 agcttttatc gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt
     8881 cacgttgaat tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc
     8941 attaactgat tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa
     9001 agacggttat atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca
     9061 aactcaggaa aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat
     9121 tagtagaaag gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga
     9181 gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag
     9241 gttttccagc aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg
     9301 ctattttgct cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg
     9361 ttgcgcttgc ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat
     9421 ttggagactt actgtttcct ttgattattt tattttgttt agtaattaaa tggatcgagt
     9481 ggacaaaatg ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt
     9541 tatatcgtgt atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca
     9601 attcgctttc tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg
     9661 ctttattgat cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg
     9721 ggatagcagt cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag
     9781 tcgcgttgat tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc
     9841 gtgcgccatt tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg
     9901 cggctgtctc tgaaaaagtc aatttgttta ccggtcgaga agatatttgg cataaattct
     9961 atcagacgct aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc
    10021 agcgaggaaa ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg
    10081 gattgatagg atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt
    10141 tgaaagcaga tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga
    10201 tgcagtcatt tatggaggat acattgactt catacccatg ggttccgatt atttatatcc
    10261 tgttggctat ggccgttcat cgatatgatg aaccagaagt tacggaggaa ttctatgtta
    10321 tggaagaggc atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata
    10381 gcgctcattc ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg
    10441 tgacaaccgt cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag
    10501 aaacgttaga gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg
    10561 taccaaaaat atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac
    10621 acaaatgttt aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattact
    10681 gctacagttt tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt
    10741 atgggcgttt atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg
    10801 actgaaagca caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat
    10861 gattccctgg ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata
    10921 tacttattta gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct
    10981 atccaatctg tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa
    11041 tggtatatat ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc
    11101 tttcttgaca ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat
    11161 gcaattaatt acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc
    11221 taatcagttt gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt
    11281 tcaaggttat cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac
    11341 tttgtatttc tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag
    11401 attgaaacca catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt
    11461 agactaaaag atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt
    11521 gaagccctca atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg
    11581 aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa
    11641 tctacaatgt tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc
    11701 gaaggaaaaa tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc
    11761 tatgaaaata tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa
    11821 atggtcgatg atatcattga atttgctgac atcggcgacc atatttatca acctgtaaaa
    11881 acctattcaa gtgggatgtt cgttcgtcta gcttttgctg tagcgatcaa tgtgaatcct
    11941 gatattttga ttgtggatga agcattggca gtaggagatc ttgaatttca actaaaatgt
    12001 atggaaaaat ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat
    12061 attagttcga ttcgccgttt ttgtgatcgt tcctattggt tgaaaaatgg aaaagttgtc
    12121 gaatatggtg atacgatgga agtaacgacg aattacgaaa actttttgaa gaaaaaatca
    12181 gttaaaacag tagatcgaaa taaaaaccaa gtagaacgct tcgcagctcc agatatcgta
    12241 gacgtgacga aagccgaatt gttggataaa gataaaaatc cagtggatat gctggaacaa
    12301 ggagaaaaac tatacgttaa agtaacctat gaagtaaaag acgatacaat caaaaatcca
    12361 gttttaggaa ttgctctgcg tacagtcgac aatttttatg tgtgcggact caatactcta
    12421 ctagatggta tcaagatccc ttggaaaaaa ggtgagaata tcttttatct ggagtatgaa
    12481 aagttaggtt tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca
    12541 acggttcctt tggtatacaa gacaaaatac atgaacttgt ttgtcagcgg ttcttatatc
    12601 ggagaaggca tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata
    12661 tgattttgaa atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa
    12721 accaggaagt aaagttttag agtttggtcc tggaaacggc cgattaacga aacatttgat
    12781 cggagcaaaa caatgtgaag tgagcattgt cgagctagat aaggaattgt tcgattttgt
    12841 cagcgagttt gcccaagatg gattttatgg agatatcgaa agttttgaat gggcaaacta
    12901 ttatgctgga caaacatttg attacgtttt gtttgccgat gttttagaac atttggtaga
    12961 tcctggaaaa acgttgaaga aagttagaga atttttgaat gaagaaggag aaattctaat
    13021 tacattccct aaccttgcgc ataattctgt catgattgat ttgttcaata atcaattacc
    13081 atgggcttct tatggtttgc tagatgaaac ccataattct ttttatactc atgatggctt
    13141 ccaaaaagtt tttgaaaaag caggattata catcaatatt gaagattatt tgtacttagc
    13201 tgtaggtgat acagaattaa aatcaactta tgaagaattg ccggaagctg tacgttatga
    13261 tttcaaaatg cgaccatttg gcgaagtcta ccaatacttc ttctcattga tgaaacatcc
    13321 agtagcacaa agttccattg cgcagccgca aaattctaat tatgtgaaag tattagaagt
    13381 aatccagcaa acgaaacaag atgaaataat tcaacaaata ccattcaata atttcacagg
    13441 ggaaaatgag acactaagtt tccatgtatc agaattagca gaacgaatgg tgtttcgctt
    13501 cgcgcagcaa cctagcttta tcgaattcag cgcagaagct gctggagaaa aactcacatt
    13561 tatcgattca aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atggtaaaga
    13621 tttaccagag ttctttttaa cggatattgc agggaaagaa gtgactatcc attgtcacta
    13681 tcgttttatc ggagaattga caccaacaat gaaagaattg ctggaaacaa tccgtccaat
    13741 ggctgaagtt acaaagcagc tttctgagaa aaatgatgaa ttagaaagag aaaatcatta
    13801 tcttattgag aataatacaa gattagatca gacgttgaaa acaaccataa atcgctattg
    13861 cacgttgttg gaaagtgatg aatggacaat caaacataga ttagggcgac gtaagaaaga
    13921 aacatcgaaa aaaatccaag aaaaagagct ttctctatgt attgacgaaa aaatctggga
    13981 tgcagaaaca aagatattga aaatcatcgg gtggggaatc gctaattcgg atcgccaacc
    14041 attgtcctat aagttatcag ccgaccaatc accatttttt gaagcaattc ctgttttacg
    14101 agaagaagtc aatcaagcaa aacagctagc tgaaggagca aaagcaggat ttgaactgcg
    14161 gatcctttgc gagaaggaac gttcattctt agttgaagca gtggctcaaa atggtcaatc
    14221 atggatcatt gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg
    14281 caaaaaaaag aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat
    14341 ttgcttattg tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta
    14401 gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta
    14461 tataatcttg atgttaagac ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga
    14521 ggaaaagcaa tcttagactt ccaaacagca agacatcaaa atggcatagc tgtgaaatta
    14581 aacggtaaat atccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta
    14641 ctgaaaaaag ggataaatta tgcaaggact catggcgtaa aaaaagcaat ccgccgggtc
    14701 aaactggaat tgaaaccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa
    14761 cctgatctga aagagcaaag gaaagaagta caaggatttg cctaccgccc attgatttcc
    14821 atacttatgc ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta
    14881 cttgatcaaa cgtatgatca ctgggaacta tgtatcagtg atgatgcttc tactgatcca
    14941 gctatccgaa agtgtctaga atcctatcaa gcaaaagatg atagaattaa agtcgttttc
    15001 cgacaagaaa atggacacat cagtctggca actaattctg cattgaaaat ggcagaagga
    15061 gagtttatag cgctcttaga taatgatgac gaattacctc catttgcctt atatgaagtg
    15121 gcaaaagtgc taaacgtaca tccagaactg gatttgatct acagtgacga agataaaatc
    15181 gatgcagatg ggaatcgctt tgacccgcac ttcaaagcag actggtctcc cgatacattg
    15241 atgggaaata attatatctc tcacttaggc gtatatcgga caagcattgt taaggaactt
    15301 ggcggtttcc gtaaaggata tgaaggctct caagattatg atttggttct tcgtgtgaca
    15361 gaacaaattc ctgcagaaca catttaccac attgaccgtg tcttgtatca ttggcggacg
    15421 atcccgggat caactgcaag caatggagaa gctaaaagct acatctatga ttctggagtg
    15481 aaagcattga cagatgctct atctagaaga aatatcaagg gaagcgtaca tcctggcaga
    15541 atttctggtt tttatgaaat cacttatgat gttttgcaag aagacttggt cagtgtgatc
    15601 attccaacta agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa
    15661 acgtcttatc caaattatga aatcattatt gcagataatg gaagtacaga tcctaaaatg
    15721 caagaattat ttgcagaata caagcatcag ctgaaagacc gatttatcgt cgaattaatt
    15781 gacattccat tcaattattc acgtatcaac aatctggcag cagaaaaagc aaacggcaaa
    15841 tactttttat tcttgaataa cgatacagaa gtgattgaac ctgactggat gacagcgatg
    15901 gtttcttatg cacagtttga tcgaatcggc tgtgtaggag caaaattatt ttatccggat
    15961 gatacaacgc agcatgcagg tgtattatta gggatcggtg gcgttgcagg acatgcattg
    16021 aataactatg atcgaacaca ttgtggttac tttggccgat tggtcattga cgtcaattac
    16081 ttagcagtca ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgtcaatgga
    16141 tttgatgaaa tattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa
    16201 ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca
    16261 agaggttatg aagatacacc agaaaaacaa agacgttttg ctggggaaat caaaaaaatg
    16321 caagataagt ggccttctta tattgctcat gacccatttt ataacgataa tctaactaaa
    16381 gaaggaatcg gcgatttttc gttgagacct gattgattaa aagaactgta ttttcatttt
    16441 tattaatgaa aatacagttc ttt
//
LOCUS       DAHEYX010000047        16339 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHEYX010000047 DAHEYX010000000
VERSION     DAHEYX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.047"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(229..1956)
                     /locus_tag="MB219_001032"
     CDS             complement(229..1956)
                     /locus_tag="MB219_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCC8426762.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1949..3142)
                     /locus_tag="MB219_001033"
     CDS             complement(1949..3142)
                     /locus_tag="MB219_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCC8426763.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3329..3973)
                     /locus_tag="MB219_001034"
     CDS             complement(3329..3973)
                     /locus_tag="MB219_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8426764.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4067..4201)
                     /locus_tag="MB219_001035"
     CDS             complement(4067..4201)
                     /locus_tag="MB219_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCC8426765.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4203..6335)
                     /gene="feoB"
                     /locus_tag="MB219_001036"
     CDS             complement(4203..6335)
                     /gene="feoB"
                     /locus_tag="MB219_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCC8426766.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6332..6805)
                     /locus_tag="MB219_001037"
     CDS             complement(6332..6805)
                     /locus_tag="MB219_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCC8426767.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7206..7430
                     /gene="nrdH"
                     /locus_tag="MB219_001038"
     CDS             7206..7430
                     /gene="nrdH"
                     /locus_tag="MB219_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCC8426768.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7589..9748
                     /gene="nrdE"
                     /locus_tag="MB219_001039"
     CDS             7589..9748
                     /gene="nrdE"
                     /locus_tag="MB219_001039"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCC8426769.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9798..10763
                     /gene="nrdF"
                     /locus_tag="MB219_001040"
     CDS             9798..10763
                     /gene="nrdF"
                     /locus_tag="MB219_001040"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCC8426770.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10852..11991)
                     /locus_tag="MB219_001041"
     CDS             complement(10852..11991)
                     /locus_tag="MB219_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCC8426771.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12300..13013)
                     /locus_tag="MB219_001042"
     CDS             complement(12300..13013)
                     /locus_tag="MB219_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCC8426772.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13267..13968)
                     /gene="nagB"
                     /locus_tag="MB219_001043"
     CDS             complement(13267..13968)
                     /gene="nagB"
                     /locus_tag="MB219_001043"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCC8426773.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14202..15734
                     /locus_tag="MB219_001044"
     CDS             14202..15734
                     /locus_tag="MB219_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCC8426774.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15925..16104)
                     /locus_tag="MB219_001045"
                     /pseudo
     CDS             complement(15925..16104)
                     /locus_tag="MB219_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16124..>16339)
                     /locus_tag="MB219_001046"
     CDS             complement(16124..>16339)
                     /locus_tag="MB219_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCC8426775.1"
                     /translation="SEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFE
                     SILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5054 a         3379 c         2861 g         5045 t
ORIGIN      
        1 acttcgtaca taataaactt taatcacatt agtctttcat acctacgatc gatctctttg
       61 actcgcaccc ataaattcat aatagtttgt cggcgtcttt gaacgtttcg aagatgagct
      121 acgcattctc tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg
      181 gatatttgat ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca
      241 gctttcgact aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt
      301 tccattgttg atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc
      361 taatactttg taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat
      421 ctgctcttcg cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc
      481 aaacatccaa tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg
      541 acctagtgca acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt
      601 gtcatatttt tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc
      661 tatgattttc ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc
      721 tggcactgct aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc
      781 tttatattgt gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc
      841 tgactcgcca tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac
      901 atcatagccc atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc
      961 ccattcaaca taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat
     1021 accaatcgca cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt
     1081 acttttctgt gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt
     1141 aaaacggtca atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc
     1201 gacttgtgct ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat
     1261 agaggcaata acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat
     1321 cgtactttta gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa
     1381 tgaatcagaa gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg
     1441 aacggttggt ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc
     1501 gaaaaatgca actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag
     1561 gatcggtttg ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac
     1621 cccaatgatg atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc
     1681 attccataga ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc
     1741 tggacgtttc ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc
     1801 tggaatcact aacagtaaat ttgtaattcc cgacatcact gcctgtccaa tcgtttgtaa
     1861 aaatgaaaat aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt
     1921 tgccactggt aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg
     1981 ctaatgcttc aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa
     2041 ctgcaagtgg agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt
     2101 tcccaatcac cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag
     2161 cttctaacgc ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca
     2221 tcagcatgct gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt
     2281 cgatattcgt cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat
     2341 cgacttcttc cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac
     2401 ctgttccgat ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta
     2461 aagcttcatt caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt
     2521 ccagtaattc gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt
     2581 ccatcagcaa gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca
     2641 tcccaccaga taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta
     2701 atgctttttc tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt
     2761 attctgtatt ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat
     2821 tgattttatg acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc
     2881 catcaatata gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg
     2941 tggatttccc actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa
     3001 aattcacgtc atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct
     3061 ggtgatcatt catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat
     3121 gttttacttc tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc
     3181 tgaaaaaagt gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa
     3241 tttgcttgtc caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa
     3301 gttaatattt ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa
     3361 agtttgtcat acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg
     3421 taaatttagc ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa
     3481 ctgtggcgcc agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg
     3541 ctttttctga taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg
     3601 tttgattgac cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct
     3661 ccacacttgc catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca
     3721 ataggaaatc ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt
     3781 ccatgattcc aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat
     3841 tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt
     3901 caacgatacg ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt
     3961 tctttccaga cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct
     4021 agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct
     4081 gttccttttt tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac
     4141 gagtatagac aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca
     4201 tcctattcct ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga
     4261 cggaacaaaa cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct
     4321 acctgtccac cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct
     4381 aaaccgcatt gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg
     4441 atagccccaa ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa
     4501 gctgctgcgg gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat
     4561 acttccgcat gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat
     4621 cctgtaatcg ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact
     4681 gccaggattc gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa
     4741 aaattataag aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct
     4801 tttttaacaa aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga
     4861 aaatgatatg cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt
     4921 tttttcaagc tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga
     4981 gctacccaag attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt
     5041 gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca
     5101 atcgtgcgac tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg
     5161 aaggattttc ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc
     5221 gccatatatc cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt
     5281 agaaatccta acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc
     5341 catcccgcta cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca
     5401 aacaatgtgt cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa
     5461 tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc
     5521 tgatcgattt tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta
     5581 catagttggg taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc
     5641 aatagtttct ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc
     5701 tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc
     5761 ttgccgcttt gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga
     5821 tcatatgtac agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg
     5881 attagttttt cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga
     5941 ccataggaaa gcttttctag attgattttt tttccttctt ttttgatgac atccatcata
     6001 ttcagaccaa tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt
     6061 cgttctaaat tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa
     6121 taatctcttg caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg
     6181 tcctgaatat gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg
     6241 cccggccaat tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc
     6301 ccgctgtttg gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga
     6361 ttagttcggc ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg
     6421 gatctcccag aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat
     6481 ccattaaccg tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc
     6541 ctgcttttaa ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa
     6601 agatttgacc tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg
     6661 cattgttgcc tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt
     6721 gcaaatggcg ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct
     6781 gctttgcctg cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta
     6841 aaatcattca ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc
     6901 tcacatagaa tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa
     6961 tctttagtat tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt
     7021 tttctctatt aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa
     7081 acacaaaact tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact
     7141 tttttactaa gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac
     7201 tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac
     7261 ggtttttaac agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag
     7321 cagtagactg gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag
     7381 aaacagttat cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa
     7441 agaatcaaaa cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa
     7501 acttccaaat ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc
     7561 aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta
     7621 tttcaagctg aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga
     7681 taaagaagct ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttcc
     7741 atctattatg gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat
     7801 tcaattgtat tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt
     7861 ttctttcaaa tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa
     7921 cgacggttcc gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt
     7981 tgcgaacggg gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata
     8041 tcagccagct acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc
     8101 ttgtttcttg actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc
     8161 tttgcaattg tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc
     8221 cggcgctcct atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt
     8281 attcgaagat agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt
     8341 ttatttgaac gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc
     8401 agatgaaaag attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta
     8461 tgaattgaca cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga
     8521 atatggtgtc ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa
     8581 tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt
     8641 acaacaagaa tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc
     8701 aatcgatggc aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc
     8761 ttctgtgatt aacggcaaac aggaatatga agtattagga acagatatct catgtaactt
     8821 aggctcgaca aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac
     8881 gatgactcgt gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat
     8941 ccaaaacgga aataagctga atcatacgat tggattaggt gccatgggct tacacactta
     9001 tcttgcaaaa gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta
     9061 cttcatgctg ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa
     9121 acaagttttc cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata
     9181 cgtaactgga gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt
     9241 tatcccaact gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta
     9301 tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag
     9361 tgcaagtatt catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa
     9421 aatttattac ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta
     9481 tgatatggac atgcgtaaag taatcgatgt ttatgcaacg gcacaacaac acgtcgatca
     9541 aggcatgagt ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa
     9601 gacaacaacg aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa
     9661 aggaatcaaa tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag
     9721 caatcaatgt gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta
     9781 attaaaggag cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg
     9841 aagatatcat tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac
     9901 gtatcccact ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata
     9961 cagtaggaca tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca
    10021 tggatcagtt gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc
    10081 aattcatgga atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga
    10141 aaaaagaaat cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag
    10201 ctgagcgtat caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg
    10261 tctttttaga aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa
    10321 acaacaaatt ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac
    10381 atggaaccta catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac
    10441 aagaaacgtt aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac
    10501 gttatacaga agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac
    10561 gctataatgc caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg
    10621 ccaatgatgt caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact
    10681 tcttctcaca agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg
    10741 attatttgat tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa
    10801 aagagtttag cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact
    10861 tgtttcttcc agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa
    10921 taaatagatt ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca
    10981 aatcagcatg cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt
    11041 caaaggatgg atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa
    11101 ttgttggatc actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa
    11161 accgacaatg attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt
    11221 gattccgcaa atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa
    11281 aaagctcatt acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa
    11341 tgtttcgttt aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa
    11401 gtgagacaac gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg
    11461 aacggtaaat aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc
    11521 agataaactg actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa
    11581 ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt
    11641 gcttgtttca tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa
    11701 ctgaggcact acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa
    11761 taaaaaaccg aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa
    11821 catataatag ataaaaccag caacaagcaa tggtaaaaaa attgccgaaa gcattttcaa
    11881 tattggacta aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac
    11941 tagctccact gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct
    12001 ccaaggtcct ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc
    12061 atttgcttaa aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac
    12121 aaaaacacgg tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg
    12181 gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat
    12241 ttttatttgg ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt
    12301 tattgcgcaa tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg
    12361 atcacatcgg cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact
    12421 cggtagtcat caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct
    12481 tcaaaataat cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac
    12541 tttttagtcg taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg
    12601 ataaaactga tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca
    12661 attgcccata aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc
    12721 gttcccgcgc ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct
    12781 gaacgaatcg ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg
    12841 atctcaactt ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc
    12901 atacctgctg gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata
    12961 gcgatcccta aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca
    13021 ccctccagtc caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct
    13081 cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg
    13141 ttcaatcagc tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc
    13201 ctttgtcttc tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat
    13261 tgaaaattac aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt
    13321 ttgcaataca cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc
    13381 atcagctttg ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc
    13441 gattcccatt gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt
    13501 tgcattgatt gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc
    13561 aggttcattg aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg
    13621 atgtgcatcg atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc
    13681 atctggtaca aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg
    13741 acggtagctt tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt
    13801 catttcactg aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct
    13861 tcctgttgcc aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa
    13921 tgcttttttt ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc
    13981 tttatatttt ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat
    14041 agaccaaatt gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca
    14101 taagatggtt caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg
    14161 acgctttatg tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa
    14221 acaacattac ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa
    14281 atgctggcga acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta
    14341 aaatcatcac tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc
    14401 acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc
    14461 taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa
    14521 catatagcga gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat
    14581 tagaccaagg agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg
    14641 ggatcgatgt cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa
    14701 aggtgatttt agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa
    14761 ccaatcactt agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg
    14821 gtgcgtatat cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg
    14881 cagacatcga gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac
    14941 aaaaggaaca aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg
    15001 aaaagaccga agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa
    15061 gccgacaaaa acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag
    15121 ctgtctttga ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt
    15181 tagtcatcgg atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg
    15241 aaaatattgc cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga
    15301 tcggtgagat tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt
    15361 acttttcaca agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca
    15421 gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag
    15481 ttaatcaatt agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga
    15541 aacttgcaca gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc
    15601 atatcgatca agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag
    15661 taatcatcgt ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga
    15721 ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt
    15781 gtcacaactc cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca
    15841 aaaaacggca aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa
    15901 cagcttttca caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa
    15961 aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc
    16021 taaccagcca tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat
    16081 aaagtacata aacataaata cagacccgtg gtgctagtta attttaatat cctaaagaat
    16141 atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg taagccagta
    16201 atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa ttttgacaac
    16261 ctaatttttc taaggaagaa aaaacagtct caactgtttt tctttgtttt ccaagtaagc
    16321 tgtggtctag tttttctga
//
LOCUS       DAHEYX010000048        16299 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHEYX010000048 DAHEYX010000000
VERSION     DAHEYX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.048"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..624)
                     /locus_tag="MB219_001047"
     CDS             complement(<1..624)
                     /locus_tag="MB219_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426776.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPET"
     gene            complement(803..1522)
                     /locus_tag="MB219_001048"
     CDS             complement(803..1522)
                     /locus_tag="MB219_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8426777.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1653..2804)
                     /locus_tag="MB219_001049"
     CDS             complement(1653..2804)
                     /locus_tag="MB219_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCC8426778.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3021..3761)
                     /locus_tag="MB219_001050"
     CDS             complement(3021..3761)
                     /locus_tag="MB219_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCC8426779.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3765..4121)
                     /gene="rsfS"
                     /locus_tag="MB219_001051"
     CDS             complement(3765..4121)
                     /gene="rsfS"
                     /locus_tag="MB219_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCC8426780.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(4145..4738)
                     /gene="yqeK"
                     /locus_tag="MB219_001052"
     CDS             complement(4145..4738)
                     /gene="yqeK"
                     /locus_tag="MB219_001052"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCC8426781.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4722..5372)
                     /locus_tag="MB219_001053"
     CDS             complement(4722..5372)
                     /locus_tag="MB219_001053"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCC8426782.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5382..5693)
                     /gene="yhbY"
                     /locus_tag="MB219_001054"
     CDS             complement(5382..5693)
                     /gene="yhbY"
                     /locus_tag="MB219_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCC8426783.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5709..6818)
                     /gene="yqeH"
                     /locus_tag="MB219_001055"
     CDS             complement(5709..6818)
                     /gene="yqeH"
                     /locus_tag="MB219_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCC8426784.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6815..7342)
                     /locus_tag="MB219_001056"
     CDS             complement(6815..7342)
                     /locus_tag="MB219_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCC8426785.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7569..8354)
                     /locus_tag="MB219_001057"
     CDS             complement(7569..8354)
                     /locus_tag="MB219_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCC8426786.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(8347..9216)
                     /locus_tag="MB219_001058"
     CDS             complement(8347..9216)
                     /locus_tag="MB219_001058"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCC8426787.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9217..10596)
                     /locus_tag="MB219_001059"
     CDS             complement(9217..10596)
                     /locus_tag="MB219_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCC8426788.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10604..11023)
                     /gene="fabZ"
                     /locus_tag="MB219_001060"
     CDS             complement(10604..11023)
                     /gene="fabZ"
                     /locus_tag="MB219_001060"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCC8426789.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11048..11524)
                     /gene="accB"
                     /locus_tag="MB219_001061"
     CDS             complement(11048..11524)
                     /gene="accB"
                     /locus_tag="MB219_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCC8426790.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11527..12762)
                     /gene="fabF"
                     /locus_tag="MB219_001062"
     CDS             complement(11527..12762)
                     /gene="fabF"
                     /locus_tag="MB219_001062"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCC8426791.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKRDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12781..13518)
                     /gene="fabG"
                     /locus_tag="MB219_001063"
     CDS             complement(12781..13518)
                     /gene="fabG"
                     /locus_tag="MB219_001063"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCC8426792.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13530..14447)
                     /gene="fabD"
                     /locus_tag="MB219_001064"
     CDS             complement(13530..14447)
                     /gene="fabD"
                     /locus_tag="MB219_001064"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCC8426793.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14554..14778)
                     /locus_tag="MB219_001065"
     CDS             complement(14554..14778)
                     /locus_tag="MB219_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCC8426794.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14831..15793)
                     /locus_tag="MB219_001066"
     CDS             complement(14831..15793)
                     /locus_tag="MB219_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCC8426795.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15803..16243)
                     /locus_tag="MB219_001067"
     CDS             complement(15803..16243)
                     /locus_tag="MB219_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCC8426796.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4296 a         3719 c         2792 g         5492 t
ORIGIN      
        1 tgtttcagga tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc
       61 tggatattct gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg
      121 gtaaattcga ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc
      181 gccacaatcc aaaatctctg atacacgaaa tgctaggtat gcactatcga tcacattatt
      241 ttttttcgtg tctatcttct catttctttc gataagaatt ttttcaggac gatgcttcaa
      301 gctccactct aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa
      361 ggctgaatca gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat
      421 tggtgcgcgg tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt
      481 tcctaatcga tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc
      541 agttacgttc acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg
      601 ttctaaagtg atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca
      661 tttcacatga tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc
      721 acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat
      781 acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta
      841 catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag
      901 atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc
      961 ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga
     1021 tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa
     1081 ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag
     1141 aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg
     1201 ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc
     1261 caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga
     1321 ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac
     1381 gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa
     1441 tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga
     1501 tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg
     1561 caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc
     1621 ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa
     1681 ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc
     1741 gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt
     1801 aagaatcttc ttccaacatc ggtaaatcca agtagtcgaa gataattttt attttgttgg
     1861 acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt
     1921 gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt
     1981 gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt
     2041 tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc
     2101 tgcagcgaat aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt
     2161 tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg
     2221 attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca
     2281 tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca
     2341 agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac
     2401 gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag
     2461 cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca
     2521 cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc
     2581 gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg
     2641 cgatgccatt tatcgatgat agccggttcg cctcgttgca aaaaattccc gctcattaca
     2701 gcaatgacga cctctgctcc tgttgttttt cgtgccattt ccacatgata gcgatgtcca
     2761 ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat
     2821 agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc
     2881 cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa
     2941 gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt
     3001 aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc
     3061 tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt
     3121 tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca
     3181 cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg
     3241 ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa
     3301 tgatagctat attctggaaa aatttcatct acttgatagg tggagtgtac gtcaaataaa
     3361 aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt
     3421 ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt
     3481 tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct
     3541 cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca
     3601 tcttttgcaa aggcaactgc taatgctcca gtaccgcatg ccagttccaa aatatttttt
     3661 gtgtcttctt gtaaatgacg tttcgaaaaa gctagccatt gctgataaag tgtttcgtcc
     3721 atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca
     3781 ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa acttcgttct
     3841 ggtgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct
     3901 tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct
     3961 tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc
     4021 aacaaggaaa catttcgaat gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct
     4081 gcttcaaccg caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag
     4141 cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt
     4201 gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta
     4261 gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa
     4321 tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat
     4381 gcacccggat tgcatgaaga atttctacat cggtgattcc taattcccgc tctacaaacg
     4441 aagcgccaac aagaccatgc cagattgcat tattataatt caataactct ggatcaaaac
     4501 cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg
     4561 tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg
     4621 ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt
     4681 tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc atattcatac
     4741 aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag
     4801 ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc
     4861 caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg
     4921 accatagaag tcagctcatc gattttatac cattttggca aatattctac catatcccca
     4981 ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca
     5041 taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg
     5101 tcttccacag ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca
     5161 tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg
     5221 ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag
     5281 atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa
     5341 cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct
     5401 tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca
     5461 atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca
     5521 atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct
     5581 tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga
     5641 agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc
     5701 cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc
     5761 gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt
     5821 ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga
     5881 cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt
     5941 ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg
     6001 ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga
     6061 taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag
     6121 tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca
     6181 tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca
     6241 tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt
     6301 gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt
     6361 tccgtcatct catgagattt cttagcacta gttaataata catctactgg acgaattcct
     6421 gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt
     6481 aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt
     6541 cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct
     6601 ttctgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg
     6661 atctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa
     6721 cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata
     6781 acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca
     6841 tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt
     6901 gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac
     6961 gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg
     7021 ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg
     7081 caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg
     7141 aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt
     7201 ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt
     7261 tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct accatccatg
     7321 ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa
     7381 agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt
     7441 tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa
     7501 aaatttagaa acaaaaaaca actaagcgag caagaccatt ctttactctt tgcttagttg
     7561 ttaaacacct aatattggcg gaacctcttg tatctttttt ccagtaattc atgctggtcg
     7621 aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta
     7681 tatcgtggca actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac
     7741 tcgcgtagtt ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg
     7801 tctttccaca aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc
     7861 agcatccaga cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca
     7921 atgatgatgg cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata
     7981 gcctctcctt cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc
     8041 aatgtaatca ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga
     8101 tagccttcag gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct
     8161 ttttgaatac cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc
     8221 gcttcgtcat ctgcaaacaa acgatcacca tggcattcct cgaaagaatc gaagatctct
     8281 tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt
     8341 gctgtcttat ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat
     8401 tttctgcttc aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa
     8461 ttcagccttt tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct
     8521 tccagcaaaa ccgatcaagc tttggggttc tgctagaatg atatcgcctt ccattgcaaa
     8581 actggcagtt actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc
     8641 attgctatgc cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc
     8701 ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt
     8761 tgctcgttcg aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat
     8821 aaaattagga tccatcactc caataatgaa gggaatccct tgaatggttg ctttaccagt
     8881 gaggactgct tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc
     8941 gggaaatgct agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc
     9001 atcaataatc aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg
     9061 gcatactttt tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt
     9121 tgcccacatg ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt
     9181 gatccgaata tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc
     9241 tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat
     9301 gactgcaggg tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt
     9361 gataaattca cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg
     9421 gacgattatt tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac
     9481 tgcactgtct acccgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc
     9541 tggagaagga gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg
     9601 tccttggaat actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac
     9661 ctgttttttt actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat
     9721 tcgagtattc atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt
     9781 tccggcattt tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc
     9841 tctttttgtt tgtgagatca ctacagaagg agattcttcc agtacttttt gattattacg
     9901 ttgcaaagaa caatctcgtt ctcctaaatg aatgacattg ccaaaatgat cacctaaaat
     9961 ctgaacttct atatggcgag caggataaat gattttttcc aaatacatat catcatttcc
    10021 aaatgccgct gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga
    10081 ttgaactttc cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata
    10141 accaattctg tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc
    10201 tggaatgact gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat
    10261 tgcatcgatc gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga
    10321 ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt
    10381 cacgattgca gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg
    10441 gccaatgcag attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga
    10501 atatatcgct acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat
    10561 ttcaccgcgg ttagctatca atactttaga aaacatattt cacctatcca atcataaagg
    10621 ttaattccgc ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg
    10681 ctgaagcttt cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt
    10741 tcttacggaa ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt
    10801 caagagaaag taatgcaact gctcctgctt gagccatcgc ttcaacaatt aatacccctg
    10861 gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt
    10921 tcttagcaac gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg
    10981 gataacgatg tggaatgatt tcttttattt cttgtatgtt cattgatttc cctctttcct
    11041 gctttgctta tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt
    11101 tttctacaag gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt
    11161 tcatcgcttc aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga
    11221 aagctggttt gtcgggcgcc ggttgcagat aaacgattcc cacgattggc gaaacaacct
    11281 cgattccatc aaatactgcc gattcttttt ctttcattgt ttctggagca gttgttgctt
    11341 gttcaggtat gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat
    11401 tcatgcctcg acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat
    11461 cgaattccgt tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat
    11521 ccaaaattat tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa
    11581 tgaattactc attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa
    11641 gtcaatctca ggatctcgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa
    11701 tgccagcaaa gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat
    11761 cgatttcgtg ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc
    11821 ttttgcttct gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac
    11881 ttgttccggc tgtacaccag cttcttccat cgcaagcttc attgctttag acgcaccaga
    11941 accatctgga gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaatgat
    12001 ttctccatag attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac
    12061 acctgctcct tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg
    12121 attaggatca gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc
    12181 acaaatcgtt gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt
    12241 acggaatgct tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca
    12301 aatacctttt gcacctacgc gcaaagcaat atttccagct gccatgttag caatcgccat
    12361 tggtacgaac attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt
    12421 ttcgattgtt ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt
    12481 cattttttct ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata
    12541 gatcgaaaac atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa
    12601 atctttgact tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg
    12661 tgcgatgcca ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat
    12721 aggtgaagtg actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc
    12781 ttatccgtgc atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt
    12841 ttgtgctaag aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg
    12901 aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt
    12961 gatgaatcct ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac
    13021 agattttgtt aatcctacta cgccagcttt actagctgca taatttgcct gaccaatatt
    13081 tccaatcaac ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat
    13141 ttttttcaat acgtgctgcg tcatattgaa ggttccaatc aaattgatat caagacattt
    13201 tttgaaatct tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt
    13261 tactaacaca tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt
    13321 ctcataatca gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc
    13381 ttctatcttt tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt
    13441 tgcaaaagct aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt
    13501 ttttcctttt acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc
    13561 tgcttattct caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt
    13621 aatacttttc cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg
    13681 atactctcat agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt
    13741 tctttcttca ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat
    13801 tgaatctgac ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga
    13861 aaaggcccgc tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa
    13921 acagcttcgt ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa
    13981 tttgcaggag atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca
    14041 atcggcgcat tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata
    14101 taagcgcctc tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct
    14161 actaatgccg aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc
    14221 tccttcaata agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc
    14281 gtctgatcca aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc
    14341 tcacttgctt catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata
    14401 ccttgatatt gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat
    14461 atatccagaa tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag
    14521 gaaatgtccc agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt
    14581 cactaaatct tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc
    14641 ttcaatttca ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat
    14701 gtcagtagtc aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg
    14761 aattttttcg aataccataa taatatattc ctccgatatt gtttgctttt tatttttata
    14821 atttaacagt ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg
    14881 taaatattac tttctgctgt tttcccaagg tgatctttcc actagaaact gcttcatcca
    14941 ataagatagg gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact
    15001 tttcttgcgg catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat
    15061 gcaaaagcag ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt
    15121 ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg
    15181 aatcttctga atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc
    15241 tggtaccgtc agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga
    15301 gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta
    15361 gcttagatag tgtttcacca ccaatcacta gtcccttagt tgagccgcag cggatcattt
    15421 tttcagccat gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga
    15481 tagcatgagt tgctcccaat ttcccttgta cttgtgctgc tacagatggc gtcgtgtaat
    15541 cgggcgacat tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt
    15601 ttatcaattt ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc
    15661 ttcgttcacg aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg
    15721 aaagatcatt attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc
    15781 caaattgttc cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca
    15841 gtgcatccag caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca
    15901 tttcacgatg aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca
    15961 actttacaac gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta
    16021 acttgttaat agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg
    16081 aagttgtttt tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca
    16141 gatctttgaa ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga
    16201 cactaacaag ataatcattt actgttttta aatctggttc catgttccac cgtccactaa
    16261 ttagtttgat tatcaaatct tttgatgttc aaactatat
//
LOCUS       DAHEYX010000049        16021 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHEYX010000049 DAHEYX010000000
VERSION     DAHEYX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.050"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            146..610
                     /locus_tag="MB219_001068"
     CDS             146..610
                     /locus_tag="MB219_001068"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCC8426797.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(672..1715)
                     /locus_tag="MB219_001069"
     CDS             complement(672..1715)
                     /locus_tag="MB219_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCC8426798.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(2092..3057)
                     /locus_tag="MB219_001070"
     CDS             complement(2092..3057)
                     /locus_tag="MB219_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCC8426799.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            3291..4136
                     /locus_tag="MB219_001071"
     CDS             3291..4136
                     /locus_tag="MB219_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCC8426800.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            4327..5514
                     /locus_tag="MB219_001072"
     CDS             4327..5514
                     /locus_tag="MB219_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCC8426801.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            5520..5876
                     /locus_tag="MB219_001073"
     CDS             5520..5876
                     /locus_tag="MB219_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCC8426802.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5878..6216
                     /locus_tag="MB219_001074"
     CDS             5878..6216
                     /locus_tag="MB219_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCC8426803.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    6292..6548
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6611..7645
                     /locus_tag="MB219_001075"
     CDS             6611..7645
                     /locus_tag="MB219_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8426804.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7638..8324
                     /locus_tag="MB219_001076"
     CDS             7638..8324
                     /locus_tag="MB219_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426805.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            8348..9196
                     /locus_tag="MB219_001077"
     CDS             8348..9196
                     /locus_tag="MB219_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCC8426806.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            9393..10166
                     /gene="sufC"
                     /locus_tag="MB219_001078"
     CDS             9393..10166
                     /gene="sufC"
                     /locus_tag="MB219_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCC8426807.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            10179..11465
                     /gene="sufD"
                     /locus_tag="MB219_001079"
     CDS             10179..11465
                     /gene="sufD"
                     /locus_tag="MB219_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCC8426808.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            11462..12697
                     /locus_tag="MB219_001080"
     CDS             11462..12697
                     /locus_tag="MB219_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCC8426809.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12684..13154
                     /locus_tag="MB219_001081"
     CDS             12684..13154
                     /locus_tag="MB219_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCC8426810.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            13159..14550
                     /gene="sufB"
                     /locus_tag="MB219_001082"
     CDS             13159..14550
                     /gene="sufB"
                     /locus_tag="MB219_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCC8426811.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14645..15787)
                     /locus_tag="MB219_001083"
     CDS             complement(14645..15787)
                     /locus_tag="MB219_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8426812.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(15826..>16021)
                     /locus_tag="MB219_001084"
     CDS             complement(15826..>16021)
                     /locus_tag="MB219_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426813.1"
                     /translation="FLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNINKLEQ
                     TISSIGKKYTKKKMKGGDTI"
BASE COUNT         5214 a         2993 c         3411 g         4403 t
ORIGIN      
        1 ggtattcttt ttgtaaacta acgtatttag tttaccatgt ttcctcggtt tatcaaactt
       61 ttaagaacaa tttttattag ttggcaagta gttttggata gagaaaaagg aggaagttcc
      121 agtctattga ctaggagcaa acaaaatgaa aatacacaca cctatcggct ttttatggtt
      181 agatgcagat gaatccggat tgactgctgt atcttttcag ccaatttcag aaagcgaatc
      241 agcgaatcag cgaattttaa cgcgcacaca agaacagttt gaagaatatt ttgcgggaaa
      301 aagaaaagaa tttgatgttc ctttatctat aaagagaggt acgccttttc agcaaaatgt
      361 ctggaaagct ttgtcgacca ttccttatgg agaaatccga acgtataaag aagtagccgt
      421 tgctgttgat agtccgaaag ctgtgcgagc gattgggcag gcaaatcgaa ccaatccatt
      481 gccaatcgtt attccttgtc atcgagtgat cgggcaaaat ggaaaactga caggatatat
      541 gggaaacgca atagatgggg ttgaaatcaa acgccaattg ttgttgttag aaggatatct
      601 gcaaaattaa aaacagttgt gattcggaca ttgttccatg cgagaatcac aactgttttt
      661 tcatgttcat tctaaacttt gatcagccaa ataaatagta aagccatcac tgtttggaga
      721 agcccgacag ctaatccata agataagaag cgtgtacctt ctttcatgaa ttttgcaaag
      781 tctaaacgca gaccaattgc cgctaaagca gtgatctcaa accagctgct gataaaatgt
      841 gccgattggt caataactac aggtaaagaa aacaagctgt tgaccaaaca aaagatcaca
      901 aaccctaata cataccaagg aagcggtaat tttcgttttt tctgcgtatc agttggcgtt
      961 tcaaacagag ctggttgttt cttgtgcatg aatttttcaa acagatagac aacaacaacg
     1021 agcatcataa tacgtagaat cttaaatagc atggcgaatt cgacaacaga attgtcaagt
     1081 aagcttgccc cagcgactac ttgaccaaca gattggagtg taccacccaa aagtgcactt
     1141 ttttcaatga gatttccacc aaacaactgt aatccaagaa aagggagagt caacatcatc
     1201 acagtaccca gtagattgac tagtgtgatg atctgtcctt tttcttcctc tttcgcatgg
     1261 atggaagggg cgattgaagc aatagcagaa gaaccacaaa cagcgtttcc tccagccatc
     1321 atcaaagaag aagtatcgct gaattgcagt tttttaccaa tcaagtaagc accgatgata
     1381 gtgactgaca tttgcagtaa cacaaaaaca attcctttta caccaatttg agcaatcgtt
     1441 tgagctgtga cagttgcacc tagtaaaaaa acagagaatt ctaatagttt gctttcagca
     1501 aattttgtgc cgattgctag aacaggctgt ttaaaaaaag tatttcctaa taaaataccc
     1561 aataaaatag caatagtcgc tccacctaac tgtggcagat aaatagcaag aagcctgctg
     1621 aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga tttcgcggac cgctgaatcc
     1681 tttacagatt ttattggatg tgacaatgat ttcatttgat cggttccttt cgtcaataaa
     1741 tactaaaagc atactcgaac atagtagcac taaaccagta ttttgacaaa agaacaagag
     1801 gtcgtgacaa aagtcttgta cgacctcttg tttcgaataa acggtgttcc agaagtagct
     1861 tctacggaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc
     1921 ttatttcttg aagctgacca cttcttttac gacctcttgg taaacggtgt tcaaaagctt
     1981 gcatctgcgg taataagccc aaccagtaca aaaacatgaa cagctgtttt tgtttggccg
     2041 ttcttattac ttgatgcaga acagcttttt cacaacctat tgaatcgatt attaagcgat
     2101 ttttttagaa acaaattttc catgtttgct atgttcttta attaagaaac gtacgtaatg
     2161 agcaagaacg tagaagacaa ctacacttgc aaacagacat tccgcaactt ttaagaaatc
     2221 caatacgcta ctatagatac ctaaactgtt gaagtaatga acagtagtaa atatagctgt
     2281 tgcagagata acaagcggga atgtaaaggc tgcatagctt ggataaaaac gaagcttgaa
     2341 caaacgtgga agcatagaca gcacaacgaa atacaatact tgagataaag cgaacaatgc
     2401 tacgacaaga taaaaattag gttcagcaaa gtttttaaga taacctgtta ggcaaagaga
     2461 accaggtgct gcaacgatcg taatcagcgg acgtgtcgga tgagccatat ttcttacgaa
     2521 aaagacacgt ttgatgatga tcggcaataa gaacgcatag cagatcaatg aaatccaaaa
     2581 gaaaattttt ccaataagtg ggaagaagtt cccggcagtt acagtgatga ccccaaatcc
     2641 aacgtaaatg ataaaccagc ttggataaat tgcttcaatt gaaagatcag ctttgataac
     2701 atggtgataa ttgaaataaa agacaaggat cacttggaag ataacagcgc acagccaaat
     2761 gaatttgaca attggtgcaa tactttcata tgaaacaaaa taagtacaaa taaccatgag
     2821 tgacatcgta aaagttggtg aaacggaggc gatgattgga tcatgcaacg tttgtttcgt
     2881 gtgttcgaat aagataaaca atttaacaaa aatgaatatc ataagaatca tagagatacc
     2941 gccagttaga ttgcctaaag caacatggtg atagtcttta aacagatttc caagggaagc
     3001 taacccaagg atcaacccgc agatcgggat cggaataatt ttcaaaaagt tcttcatata
     3061 taatattctc ctttttattt aatagatttt agtaccaaga tgaacgaata cattgtaaaa
     3121 tgaataggaa tgtatgatta cgagcaagca gacagctgat tgtcttcatc taagttacta
     3181 gttggatcag caactgtttt gttgtacatt tctattatag atggtttttt gtatatttaa
     3241 atcaaatagt ttttatagta attatcataa aaagttatgg aagtggtgcc atgtttaagt
     3301 tattaacgac ttttcgagca gtatatgaaa caagaaattt ttccagagca gcagaagtac
     3361 tgtttttatc tcaaccagca gtctcgaatc aaatcaaaca gttggagcgg gaattgaata
     3421 tcgatctttt tcaacgtaac ggaagaaaag agatgcttcc aactaagcaa gccgacttat
     3481 tatatgaacg gacactggtt ttgttagaag aatgggaaga aactcagcaa aaattgctca
     3541 atgaacaaaa tgaagaagaa gtatgtcgga tcgttgcttc ccacaccttt gctgtctatc
     3601 ttttgcctca gttgatgaaa catctgatca aaaggtttcc taaagtgcat tttgagatcg
     3661 aaatcacgaa ttctcatgaa tcattagaac aagtagcgaa acatgaatcc gattttggtt
     3721 ttatcgaaaa gccattagag acttccggcg tacaaagata ctctttgatg gaagaccagc
     3781 tagtcatcgc tggggaccca aaaagcaaat tgtggcttgt aagagaagca aactctggtg
     3841 tttttcatta cacgcagcgt tattttgaag agatgaatat ccaagataag aagatgatcg
     3901 tgaaaagtaa cgatgtaatt gttgcgttgc tgaaggaagg aatcggtcgc tcgattttgt
     3961 ctaagcgctc tgttccagaa aatgtgccaa tgactccgtt gaatttccat cgatattttt
     4021 atttcatcca acgaaccaat ctggtttctg atcagctgct agagattgcc gaagagatcc
     4081 ttcgatacaa tgaaagcaca gaaacttctc acagcgagga gaatctcagc tggtagataa
     4141 ttgcatgtat caaaaaaagt tctatgatac actaaagata agcagtaggc ttttgtactg
     4201 catttgctca agcaactgca caaacggaaa gttaattttt cccaaatgtc tatggaaaga
     4261 ctttccatta tctaactgga ccgctataat aatcagcagt atggttttta agaaaaggga
     4321 tgaaacatgg aaaaaaaatt aaaagcttcg aacgataatc gtattgatta tggcgtgatt
     4381 ttaccagtct ttctgttatg tttgattggt ctgctctcat tatatgtagc acttagccat
     4441 gatccgaaca atccagcagt cttgaaaggc gtagggatgc aagttgtctg gtatttagtt
     4501 ggagcggcag caatcgttgt catcatgcat ttgaattcca aatggatctg gaaactgact
     4561 ccttaccttt atggtttggg gttagtggta atgggactct tgctcaaatt ctatgatcag
     4621 aatttagctg cttcaacagg gtctaaaaac tggtttcgat tcggtgcgtt tactttccag
     4681 ccagcagagt tgatgaagat tgcgtacatc ttgatgatgg cactgatcgt aactaaacat
     4741 aatacgcaag taaaagaacg gacgatgaaa tcggactttt ggctgattgg gaagctcttg
     4801 atcgtgacaa taccggtttt agcattgatc atggcccaag atgactttgg aacgatgctt
     4861 gtctttcttg cgatctttgg cgggattttc ctgatgtctg gaatctcttg gcgcatcatc
     4921 gcacctgtag taatcttggc agtagtcgtt ggagcaggaa cgatcttctt ggttacgact
     4981 gaaggcggaa gagacttgct ttataaagtt ggattcaaat cttatcagtt tgcacgtatt
     5041 gattcatggc tggacccttt ccatgatacg tcaggaatga gttatcagcc ggcccaagga
     5101 cttttggcaa ttggtaccgg tggcttgttt ggtaaaggat tcaacgtaag caatatctat
     5161 gtgcctgttc gtgagtccga tatgattttt accgttatcg gtgaaaactt cggctttatc
     5221 ggtggtgcat tcgtcatttt cttgtatttt attttaatct atcgtatgat tcgggtgtgt
     5281 ttcgatacga ataatgaatt ttacgcctat atcgcaagcg gtttgatcat gatgctgctg
     5341 ttccatgtct ttgagaatat tggagcaaat atcggattac ttcctttaac agggatccca
     5401 ttgcctttca ttagtcaggg aggatcctcg attttaggga acatgatcgg tatcgggttg
     5461 atattatcga tgagatacca aaatgaagca cctgcaagat cttcaaggag gtaagaaaat
     5521 tgtacacctt ttactggtac ccgaaatgtt caacatgcaa aaaagccaaa gcgtggttag
     5581 accagcatgg cgttagttat caaaccgttg atatgatcaa agaagtaccg aaggcctcaa
     5641 cgattgagac ttggctagaa acgagtcaag taccaatccg tcgcctgttc aacacaagcg
     5701 ggatgaaata tcgggaactt ggactgaaag atcaagtagg cgagtttacg gcttcaaaag
     5761 cgagccaagt cttatcgaca gatggtatgc tgatcaagcg accactgatc ttacgagagg
     5821 accagcttgt tgcaattgga ttcaatgaaa aaatttatga aggagaactg atataaaatg
     5881 gcaggcaagc atttgaagaa aaaggacaac ctttggatct tatttaatgg aaaagaatac
     5941 tgtgttggtt tgacaaatga agcgcaagaa gaacttggag acatcacgtt tgccaatctg
     6001 ccaaaagcag gacaatcgct aaaaaaagat gaacctctga tcgaagttga agcggaaaaa
     6061 gcggtcaatg aatttgccag cccgctaacg ggtacagttt cttctgttaa tgataaaatc
     6121 ggtgaagata tcaatatctt gaatgattca gatgaaatga atgcgtggat cttaagtttc
     6181 aaagatgttg atccaaaaga atttgacgaa ttataaaatc agaaaatcac ttgaaaaaag
     6241 ttgacactga ttcttccagt tgctataatc ataagcaatc ctatatgaaa cgagaaagct
     6301 ttgaaaagaa gagtacatgt cacggacgtt tgaagagagc cctgtttgct gaaaatgggt
     6361 aacgaaaatg agatggaaga tggtcttaga gcagagtaag cgagcgtaag caaacttact
     6421 acgggagtgc ccgttacagc gcgacagtat acggaaagaa gctaagtcca agtactggaa
     6481 gaggctgtta ttgcgaagta acggtaaatc aaggtggtaa cacggaagtt caagctttcg
     6541 tccttttgct taaggcaaga gggcgaaggc ttttttgttt gaaaaattat aagagaaaga
     6601 tgtgaatggc atgtcattaa tccaactaac gaatgtcaaa aagacattca atgggaaaaa
     6661 cggaaacatc caagccgtca atgacgtttc actaaacgtt gaaaaagggg atatttatgg
     6721 aatcgtcggc tattctggcg caggaaaaag tacattagtc cgtctcctga atggacttga
     6781 gcttcccacg agcggcgaag tgatcgtaaa cgatcaagat attacaaaat taaaaaacaa
     6841 agaattacgt acattccgaa agaaaatcgg tatgatcttc cagcatttca acctgttatg
     6901 gtcaagaact gtacttgaaa atatccagct gccgctggaa ttagctggcg taccaaagag
     6961 caaacgtaaa gaacgggcag aagaactgtt gcgtttagtc gggttagaag gacgcggaca
     7021 agcatatcct tcacaacttt ccggcggaca gaaacaacgc gtcggtatcg cacgagcatt
     7081 ggcaaatgat cctgagatcc tgctttgtga tgaggctact agcgcattgg acccgcaaac
     7141 aacagaagaa gtattagact tattgttagc gatcaataag aaattgaatt tgaccatcgt
     7201 cttgatcaca catgaaatga atgtgatccg caaaatctgc aacaaagtcg ctgtgatgga
     7261 gttggggaaa gtcgtagaag aaggagacgt attgactgtc ttcagacaac cgaaaaaaga
     7321 agtaaccaaa cgctttgtac aacaagacat cgaaccagaa gctgattcga cagaactatt
     7381 agctgaactc atcaaagaaa atccaagcgg ccgtttggca actttgacct ttagcgaaag
     7441 aaatgccaac gagccagtca tttcccaagc gattcgacgc tataacatcg atgtcaacgt
     7501 tgtgtatgga aagatcaaac aagtgaaaga aggttccttt ggttcattga ctgtcttgat
     7561 gactggtgaa accaaagaat tagatcaagc agaagtgttc atcaaagaac aaggagtagg
     7621 aattgaggtg atccatcgtg gataaaagct tttcagaaac atatctagat tttaaacaaa
     7681 tcaacccgga aacgatgaat caggctatcc ttgatacact atatatgaca ttaatctcaa
     7741 tggtcttagt gacgatcatc ggattagttt tgggattgat cctttactct attggacgca
     7801 aaaacaaacc gtatgctcga atcatttatg gaatcgtatc gatcatcagt aatattttcc
     7861 ggtcaacgcc atttatgatc ctgatggttt tgatcattcc atttactaaa gcaatcgtag
     7921 gcactatgct aggtgcgcaa gcagccattc cagctcttgt tttatccgca gcaccatttt
     7981 atgcccgtct agtagagatt gcttttcgtg aagtcagccc tggtgtactg gaagcagcag
     8041 atgcaatggg agcaagttac tgggaaatca tctggaaggt cttgatacct gaaagtattc
     8101 ccgcattgat ttctggatta acagttacaa cgatctcgat gatcggattt actgcgatgg
     8161 ccggcgcgat cggcgcaggt ggacttggag gtcttgcttg gcaggaaggc taccagcgtg
     8221 gtaatctaac agtgacattc gttgcgactt taattatttt actaatcgta tttatcgttc
     8281 aaggaatcgg tgatttctta actaagaaaa cagacaaaag ataataggat atagggggat
     8341 gtaggatatg aaaaagaaaa tttttggatt tgcagcagcg cttttgctga ctgtaggatt
     8401 agcagcatgc ggtaacaaca gcgacagcaa agattcagca aataaagcag acgacacgac
     8461 attgaaagtc ggcgcttcac cgacaccgca cgcagagatt ttggaacatg tgaaaccatt
     8521 attgaaagat gaaggaatcg atcttgaaat cgtcaaattc gatgactatg tattaccgaa
     8581 ccaatctttg gaagaaggcg acattgatgc caattacttc cagcatattc cttatttgaa
     8641 caaagaaatc aaagaaaaag gatatgactt tgtcaatgcc ggtgctgtcc acatcgaacc
     8701 aatgggcttg tactctaaaa aagtcaaaga tgtttccgaa ttaaaagatg gtgcaacaat
     8761 cattacttca aattctgaat cagactgggg acgcatcatc acaattctac aagatgctgg
     8821 cttagtcaaa gtcaaagatg gagtagatct ggaaacagca acattcgatg atattgaaga
     8881 aaaccctaag aacttgaagt tcaaccatac gatcgaccca tcacttttag cttctacgta
     8941 ccaaaatgat gaaggtgacc tagttgcaat caacgcaaac ttcgcttacg gcgcagggtt
     9001 gaatccatta gaagatgccg tattgcttga aaaggacaac tctccttacg tgaatattat
     9061 tgcgactcgt aaaggtgatg aagacagtga caaaatcaaa aaactgattg aagtgcttca
     9121 ccgcaaagaa gtccaagaat ggatcacaga caaatggggc ggctctgtta aaccagtaaa
     9181 ggctgatgca aaataaggta tggaacaaaa aagatgagaa ataagtctgt aatggcttat
     9241 ttctcatctt ttttatacct attctaattt agaaatcaaa attgtttaat aataaataaa
     9301 agatatttga ttttagtaag gaaggtcatt gcgcttcatt ctcaattagt ctaaaatgag
     9361 tgagaacgaa agagaatttg ggggtataat gcatgtctgt cttagaaata aaaaatctac
     9421 atgtgtcgat cgaggaaaaa gaaatcctta aaggcgtaaa cctaacaatg aaaaccggag
     9481 aaatccatgc catcatggga cctaacggca caggaaaatc taccttatct gccgctatca
     9541 tgggcaatcc aaactatgaa gtaacagaag gcgaaatttt gtttgacggg aaaaatgttt
     9601 tagacttgga agtagatgaa cgtgcgcgac ttggcttatt tttagctatg caatatccaa
     9661 gtgagatacc aggtatcacg aatgcggaat tcatgcgtgc agcgatcaat gcgaaacgtg
     9721 atgaaaacga caaaatttct gttatggcat ttttgaaaaa gttagatgaa aaaatggctt
     9781 tactaaatat gccagaagaa atggctgaac gttatctgaa cgaaggtttt tctggcggag
     9841 aaaagaaacg gaatgagatc ctgcaattgt tgatgttaga acctacattt gcaattttag
     9901 acgaaatcga ttcaggtctg gatatcgatg ctttgaaagt cgttgctaaa ggagtaaatg
     9961 aaatgcgcgg agacaatttc ggtgcattga tcatcaccca ctatcaacga ttattgaact
    10021 atatcacacc tgatgtcgtt cacatcatga tggaaggccg agtagtcatg acagggaatg
    10081 ctgacctagc taaacgtctg gaagcagaag gctacgctgg tatcagtaaa gaattaggaa
    10141 tcgactataa agaagaagaa gcgtaagggg gagcaagaat gaaagaaaaa aactggctcg
    10201 attatcttga cgcggtaaat gatttttctt taagtaaagg cgaacctgat tggatgagaa
    10261 ccttccgtca agacgcacta gcgaaagcag atgaactgcc attgccgcat atcgatcggg
    10321 tgaaattcca tcgctggtcg ttatttgatg tgaaagaaac acaaaccatc agtgaaacag
    10381 gcacgattcc ggcatttgat gcaatgaaag acaatcctgt gcttgtccaa caaggttcat
    10441 ggacgatttt tgaacaactg cctgtggaat tagcagaaaa aggagtcatt tttaccgact
    10501 tgttcactgc aatgatcgaa tatccagaac tagtccaaga atattatatg aaaaaagcag
    10561 taaacatgaa cgaagaccaa ttgactgcac tccatgtagc ttttatgaac agcggtattt
    10621 tcctgtatgt gcctaaaaat gtcgtgatcg atgaaccact cgagtcctta ttcattcaag
    10681 atggcgcatc cgatgagcac ttcttcaagc atgtgttgat cgtggcagat gaacatagtg
    10741 aattttctta tctggaacgt ttccagacaa ccaaagaaca agtagcaaaa tcatcaggta
    10801 atatcatcgt ggaagtcatc gctaaagctg gttcaaaaat caaatattca gccgtagatc
    10861 aattaggaga aaatattact tcttatatga atcgccgtgg gcacatttta cgagatgctt
    10921 ctgtcgactg ggcaattggt gtcatgaatg acggtcatgt gatcgctgac tttgactcag
    10981 atttagcagg agaaggtgca cacgcagaag tcaaaatcgt ggcaatcagc tcgggcagac
    11041 aaatccaagg aatcgataca cgagtaacca ataaagcccc tcatacgatc gggcatattt
    11101 tgcaacacgg agtgatccga gaaaaaggaa cattgacctt taatggtatc ggccacatct
    11161 tgaaaggggc aaaaggtgca gatgcccaac aagaaagtcg tgtcttgatg ctttctgata
    11221 aagcacgtgg tgatgcgaat cctattcttt tgatcgatga aaacgaagta actgctggac
    11281 atgctgccag tgtaggacga gttgatccag aagaaatgta ctatctgatg agtagaggat
    11341 tgcataaaga agaagcagag cgcttagtga ttcgaggatt cctcggttct gtcttaacag
    11401 caatccctgt agaacaagtt cggaaagaat tagtcgaagt aatcgaaggg aagttgaatg
    11461 gatgatcgat gctaaaaagc tacgcaatga ttttcctatc ttatttcaag acgtcaatga
    11521 tgaaccttta gtttatctgg ataatgcagc gacgacccaa aagccaaaac aagtactcca
    11581 ggcattagcg tactattacg aacacgataa cgccaatgtt catcgcggtg tccatacgtt
    11641 agcagaacgc gcgacccatg attatgaaca agcaagagaa aaagtacggg cgttcatcca
    11701 tgcaaaagaa acagcagaag tgctgtttac tcgtggtacg acgacaagct tgaattgggt
    11761 agccagaacc ttcggcgacc gctatatcaa agcaggagac gagatagtgc tctcttacat
    11821 ggaacatcat tctaatgtta tcccgtggca gcagttggca aaacgaacag gtgctgtctt
    11881 gaaatatatc gaactaacag aagaaggctt cctggatatg gaggatgcag cgaaaaagat
    11941 tacggacaaa accaaaatcg tctccattgc tcatgtatcc aatgtcttag gtgtcttcaa
    12001 tcctgttcaa gaattagctg aacttgtcca tgaaaacggc ggggtcatcg tagtagatgg
    12061 tgctcaatct gccccgcaca tggccattga tgtccaagca ctagactgtg atttctttgc
    12121 tttcagcgga cacaaaatgt gcggaccaac aggtattggt gttttatatg gtaaacgaaa
    12181 atggttagaa gaaatggaac ctgttgaatt tggtggcgaa atgatcgatt ttgtcgaatt
    12241 atacgacagt acatggaaag aactaccttg gaaattcgaa gcagggacac caaatatcgc
    12301 cggcgctatc gcattaggcc atgcaatcga ttatttgcaa aaactcggta tggacaatat
    12361 ccatcgatat gaagaagaac tagccgctta tgtgttgcca aaattacaag caattgatgg
    12421 attgactgtt tatggtccac aagacccgaa ttttcggaca ggagtcgttg cattcaattt
    12481 ggatgggtta catccccatg atgtcgcgac agctttagac atggaagggg tagccgtcag
    12541 agcaggtcat cattgtgcgc aaccgctatt gaagtacctt gaagtatcag cgactgctcg
    12601 cgcgagcttt tatttctaca acacaacgca agacgccgac cgtttagttg aagcaatcat
    12661 ggcaacaaag gagtttttcc aacatgggac tatctaaatt agacaattta taccgtcaag
    12721 tgatcttgga tcattcttcc catccgcatc atcgtggaag cttgactgac tcgacaagca
    12781 agatcgaatt gaacaacccc acatgtggag atgtgatcga attgcaagtc gatgtaaaag
    12841 acggaatcat caaaaatatc gcctttgacg gcagtggctg ctccatcagt acggcaagtg
    12901 cctcgatgat gacagacgcc gtcatcggaa aatcaataga agaagcagaa catttaactg
    12961 tagactttct acagcttgtc caaggaaaag aagtcaaaga tcccgaccaa ttaggcgatg
    13021 ctgctatgct tggtggtgta gctaaatttc ccgctcggat caaatgtgcg acacttgcct
    13081 ggaaagcact aggaaaagca atggaaaacg atggtaccgc agctttccat cattaccaca
    13141 aggaagagga gtgagaacat gggcgtacca gaattagaag aatataaatt cgggtttcac
    13201 gatgatgtcg aaccggtttt cagtacagga gaaggcttga cagaagaagt gatccgtgag
    13261 atgtcaagaa tcaaaggcga accagaatgg atgcttgaat tccgtctgaa atctctagaa
    13321 acatttaaca aaatgccaat gcaaacatgg ggccccgacc tttcagacat cgatttcgat
    13381 gccatcaaat attaccaaaa accaagtgac aaacccgctc gtgactggga agacgtacca
    13441 gaaaaaatca aagatacatt tgaaaagatt ggtataccag aagctgaacg agcttatcta
    13501 gccggtgctt ccgcacaata tgaatcagaa gtcgtgtacc ataatatgaa agatgaattc
    13561 gaaaagttag gaatcgtctt tactgacact gactctgctt tgaaagaata ccctgaatta
    13621 ttcaaagagt acttttcaaa acttgtaccg ccaaccgaca acaaattagc agcgctgaat
    13681 tcagccgtat ggtcaggtgg gacctttatt tacgtgccaa aaggcgtcaa agtcgatgtt
    13741 ccattacaaa cgtacttccg gatcaatgcg gaaaacacgg ggcaattcga acgtacgctg
    13801 atcattgttg acgaaggagc aagcatccac tatgtcgaag gttgtacggc acctacttat
    13861 tcaagtaaca gcctgcatgc cgccatcgta gaaatcttta caagaaaaga tgcgtattgc
    13921 cgttacacaa cgatccaaaa ctggtcagac aatgtgtaca acttagtaac caaacgagca
    13981 aaagcatacg aaggcgcaac cgttgaatgg atcgacggaa acctaggtgc aaaaacaacg
    14041 atgaaatacc caagtgttta cttagatgga aaaggtgcac gaggcaccat gctctccatc
    14101 gcctttgcag gagcaaacca aatccaagat acgggtgcaa aaatgatcca caatgcacca
    14161 aatacatcta gctctatcgt ctctaaatca atttcaaaag acggcggaga agtaaactat
    14221 cgaggacaag taacctttgc caaaaacagt gcgggttcga tctctcatat cgaatgtgac
    14281 acgatcatca tggacgaatg gtcgaaatcc gatacgatcc cattcaatga gatccacaac
    14341 agccaagtct ctttagaaca cgaagccaaa gtctcaaaaa tctcagaaga acaactctac
    14401 tatctgatga gccgaggcct gaccgaatct gaagcaactg aaatgatcgt catgggcttt
    14461 gttgaaccat tcaccaaaga acttccaatg gaatatgcag tcgaactcaa tcgattgatc
    14521 agttatgaga tggaaggcag tgttgggtaa acctgttata ggagctagaa cccttgatat
    14581 ataagggttc tagctttttt gttttgcctg tatactagaa atgaccagat tttgaccagc
    14641 tcttttactg atgagccatc atatcagcaa agtcttgacc tgttttctta ggtgcattat
    14701 ttgtaatatg tgcatagatg tctaatgtca ctttactatt ttcatgccct aatcgttgtt
    14761 gaactacctt gacagattct cctgcttcta ataataaact agcatgtgta tgtctaaacc
    14821 catgaggtgt aatacgaggc aagttatatt ttttcaaaat atatcttaac caatcattaa
    14881 cagtgtttgg aatgtataga gtgtttttaa ctgttgtaaa aacatgttgc tctttagagc
    14941 tagtattgta tccaagtttt aaatactctt ctttttgctg taatttccag ttgttgagga
    15001 tagaaagagt ctcagtatct aaagagataa ctcgtctaga agctcttgtt ttcggagatt
    15061 gaattataac tttaccaaat tcatctacag caagagtttt attgacatgt agttctttat
    15121 taaacgtgtc tatgtctttc cactgtaaag cgagtacttc actctttctc atacctgtgt
    15181 atgccaataa tctgaagaag gcgtattgtt tcatgttacc aaaatcttta aaagcatcca
    15241 gtaaattatt aagctcctct tttgagtaaa atttacgatt tttctcatac tctttttttc
    15301 gtggaagaag tgttttttcc ataggattat tgtcaatata ttgcattgca accccgtagc
    15361 gtaaaatttg tgcagtctgt tttctcatat aatcgtaact ttcatattta ctgtgccatt
    15421 catctaccac tttttgacaa tatgggacag agatattcga tagtttaagc ttaccaaatg
    15481 ctggaagtat ttgattattt gcgtatctta ctgagattgc gattgttgaa gctttaacac
    15541 tggtacgctg atgagttatc cattcattat acaggtcttc aaatgtcata tcaggtgctt
    15601 tctttttaac accgtataat atttgatgtc tgtactcgtc ataatctttt tgagcttctt
    15661 gtttgctact aaaacctctt ctcgtagtat ttattttttc accagttcgt ggatcggtgg
    15721 caatgtgtcc tttatacatc cacgccactt gaccgttttt ctttttgtac tttgatattt
    15781 ttgccatgaa atcatctcct cttagtttac aaggagtact taccactaga tggtatcacc
    15841 tcccttcatt tttttcttag tgtatttttt cccaattgat gaaatcgttt gttctagctt
    15901 gtttatatta tcagtaattt ctctcacagc ttcttcggga taagacatgt ctttcaattg
    15961 aataggtagc cattcatcta ccattctttc tagtagttct tctatactgc tagtgaggaa
    16021 a
//
LOCUS       DAHEYX010000050        16011 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHEYX010000050 DAHEYX010000000
VERSION     DAHEYX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.051"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            87..782
                     /locus_tag="MB219_001085"
     CDS             87..782
                     /locus_tag="MB219_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCC8426814.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(1224..2021)
                     /locus_tag="MB219_001086"
     CDS             complement(1224..2021)
                     /locus_tag="MB219_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCC8426815.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(2018..3202)
                     /locus_tag="MB219_001087"
     CDS             complement(2018..3202)
                     /locus_tag="MB219_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCC8426816.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(3227..4474)
                     /locus_tag="MB219_001088"
     CDS             complement(3227..4474)
                     /locus_tag="MB219_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCC8426817.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            4689..6434
                     /gene="dnaX"
                     /locus_tag="MB219_001089"
     CDS             4689..6434
                     /gene="dnaX"
                     /locus_tag="MB219_001089"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCC8426818.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            6454..6768
                     /locus_tag="MB219_001090"
     CDS             6454..6768
                     /locus_tag="MB219_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCC8426819.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            6793..7389
                     /gene="recR"
                     /locus_tag="MB219_001091"
     CDS             6793..7389
                     /gene="recR"
                     /locus_tag="MB219_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCC8426820.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            7453..8097
                     /locus_tag="MB219_001092"
     CDS             7453..8097
                     /locus_tag="MB219_001092"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCC8426821.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            8112..8441
                     /locus_tag="MB219_001093"
     CDS             8112..8441
                     /locus_tag="MB219_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCC8426822.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            8455..9393
                     /gene="holB"
                     /locus_tag="MB219_001094"
     CDS             8455..9393
                     /gene="holB"
                     /locus_tag="MB219_001094"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCC8426823.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            9429..10253
                     /locus_tag="MB219_001095"
     CDS             9429..10253
                     /locus_tag="MB219_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCC8426824.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            10246..10593
                     /locus_tag="MB219_001096"
     CDS             10246..10593
                     /locus_tag="MB219_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCC8426825.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            10662..11534
                     /gene="rsmI"
                     /locus_tag="MB219_001097"
     CDS             10662..11534
                     /gene="rsmI"
                     /locus_tag="MB219_001097"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCC8426826.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            11643..12764
                     /gene="dinB"
                     /locus_tag="MB219_001098"
     CDS             11643..12764
                     /gene="dinB"
                     /locus_tag="MB219_001098"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCC8426827.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(12818..13420)
                     /gene="nrdG"
                     /locus_tag="MB219_001099"
     CDS             complement(12818..13420)
                     /gene="nrdG"
                     /locus_tag="MB219_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCC8426828.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(13735..15924)
                     /gene="nrdD"
                     /locus_tag="MB219_001100"
     CDS             complement(13735..15924)
                     /gene="nrdD"
                     /locus_tag="MB219_001100"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCC8426829.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         4989 a         2987 c         3350 g         4685 t
ORIGIN      
        1 cgcataagtg atggaaaaag gtacttaaca aatgttaata tcattagtaa aatacctttg
       61 gactttaaaa agttttaaag tactgtatga tctttttaga tgaaagtttg gatgggggag
      121 catcattgga tagtagagtc ttaaaggaat atttggataa aaatgatttt ccgattatac
      181 gcaaaggata tcataaatat ttgacattcg aagggatgga agattcccat acgtatatct
      241 tgaaagaggg aacggtaaaa gcaagtgtta tttcaaatga tgggagagaa tttaaccttc
      301 gctatatcaa agatcttgaa atcgtctctc ttttaaaaga cgaatattca caattcatcg
      361 attcgccgta taatattcga gttgaatcag aatatgcaga gttttatcga atcaatcgtg
      421 tgcgcttttg gcaaaatatc aaccaagacc caaagctaca acactatgta aaagagtatt
      481 accgctatcg tctgctttat tcgatgaaaa aaatgcagca aatgctatta aacggaaagc
      541 tgggagctgt ttgtacgcaa ctttacgaat tatatgattt attcggtgtg cggacagaag
      601 aagggatgct gatcgatttt tcgatcacga atgaagaaat cgccaaattt tgtggcatct
      661 caactgctag cagtgtaaac agaattttaa aacaactgaa aaaagatggt attatcaccg
      721 ttaaagacca tcatatcgtt attttgaata tggacttgtt agaagacaat attatctttt
      781 gatgaacaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacggaa
      841 aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
      901 ccgtttacca agaggttgtg agcctgccgt ttaactctga gtcacaagga acaatttcaa
      961 aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgaggagtt ggtaggagaa
     1021 caccgtttac caagaggttg tgaaaaagct gttctgcatc aagtaataag aagagccaag
     1081 taaaaacagt ctcttatgtt tttaacttgg ctggacttat caccgtagat gtaagctttt
     1141 gaacgccgtt tatttgcaac aaaaggggct gtgacacgat ttttgtcaca gccctttttt
     1201 tgcgaacatt catttagcaa tttttagaaa cttctgccgc caccgccaaa agtcccgcca
     1261 ccgctagaat gggtagtact tccgccaccg ccgcttccag gaggtgggct tttggggatt
     1321 cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt tttcagttaa tttgatactg
     1381 gatttttctc ggaatggata tttgtagatg cctgatttca attgataacg cgaaacagtc
     1441 acaaaataaa agacaagact aaggataaga gccaaaacaa cagcaatgat gatttctact
     1501 gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt cgtctacacg ataatgtcct
     1561 cctggaactc cgtcttccac atatgttgct gatttagaca ggtaagcttt tgcagcattg
     1621 taataggcag catttgtcat ctgatcataa acatcatcta agatcgaatc gattctgcta
     1681 tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt agatttcacg ctgtcccata
     1741 tctaatagca atactgagcc attttgatca tttccgactg cgtctcttaa gtaattatca
     1801 gcaaagtctc ttggttcttc tgaattagat gtagtcgtta cgatcaagac tcgtcctttg
     1861 attttttcat tcagcttttc tgcctgttgt tccaaagatt ggatttgatc tgttgtgaat
     1921 aacccagcct catcatttac cgtagctgta tctgcatgag tagccaccgg ccagccaaca
     1981 cttactagca aaagaaagaa taaaaaaaat tttttcatca tatcaagtaa cctccgatca
     2041 gaaagaggat cgctagccca gcgaaaatcc caagagcggt cagtcccaat tttttctggc
     2101 tgatcggcag gacaccgctg acctttcctg tttgtccatt cattgcgtag tagtagactt
     2161 ttttacttga gtcattgctt cgataagtaa caagccatac cggcagtaat acgtaattat
     2221 ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt caatgttgta tatccatttg
     2281 cggtatcttt taacagcttc tctgaatagt cttttaattc agattgaatc tgtgatttga
     2341 ttgcctcata ttcgatatct cttttttctg cttggaaccc ggctaaatac tggcttttaa
     2401 aatcaattgc tttatctaac gggaaaggtt gcacgccttc gaccatcttt tgttgcgtat
     2461 tttttgataa ggcatttttt actagttctt taaaagaaag cttgcctgct cttgaaacag
     2521 caaattgctt cgtttctgtg tattcgatgt cacctatccg ccatatgcgg ataactgtac
     2581 cattcgcctg catggcacca tctacttcag catctgtagc ccaataaggg aaatacacac
     2641 ctgtcaactt atcgatttga tccttattga aaaaagcttt gggaacaaac cattttttct
     2701 tggtccaagc gagaaatctg ttgattgctt cttctttctc gacagcaaaa ggaagtactt
     2761 tatttggcaa gaattctccg cttaaacgtc ccgatagtac gacgggatta tggcagtagt
     2821 aacaataagt cgcagcagtt gtcgcatcag tcacgatctc tgcaccacag ttgggacaaa
     2881 gaaaaagatc tatcgctgcg gtattatctg cagattcgtc cgctgtatct gaaaaaccgc
     2941 ctgcctcgtc aaccgctttg cgctcggctt cattcatatc ggcaagctgt tcttgggctg
     3001 taaaagtaaa atcttcggta ggtgaaggtt cttctttgct ttcttcgcca gccatacgcg
     3061 cttctttttg cttttgctca tattggctga cttcttcttc tgtatagata ttcaggcagt
     3121 attcgcaatg aaatttttgg tctttgggat caaaagtcaa cggaccgccg caattaggac
     3181 atttatgtgt aagaacatcc atcagctgca cacctttcta aataaattaa ttaacgggga
     3241 ttcctttaaa cggtttacca cagtttggac agaatttagg gatgccgttg gaaagatcca
     3301 cgatctcatt gcattcactg catcgcattt gtagcttagg ctgaacgttt tctacaggtt
     3361 tggcagctcc gcagttggaa cagaattttc cggaattttc tgtcccgcaa actggacatg
     3421 tccaagtatc cgcatttcct tgtgcagttt gttgattttg tttttctgct tgttgtttga
     3481 tttgttcttg gttattttga gctgcttgag aaagataaga accatttgca ttcattccca
     3541 tacctacacc cataaagccg gtcattgctc ctgcttcatt ttttccggct gcttccatac
     3601 cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct aggatcacct agcatcgccc
     3661 cttcgttacg catattgatc agtttcacag aatcatcggt gtaagaaatg ctggctacag
     3721 ccacagaaac aatctccatc ccacgtaatt cacgccaaga atcatccaat gcagtatcca
     3781 tgtatttgct taattccaaa ctcttagatg gtacataaga aatccgttga ccatctgcag
     3841 acatctgatt gattgctgat tgtagcgctg ttaggaattc agctaaatac tgctcattga
     3901 tatcattgat ttccacttgt gttttgtttt tagggattgc gtttgtataa aataaaatcg
     3961 ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa gaataattct gcgttataga
     4021 aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt gattcccttt atttcttgga
     4081 gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc gccaaatttg aatcggtcaa
     4141 aagtttcagc aattgcttct ttcagtgttc cattgaacat ggaaggagct gcgtcatttt
     4201 taatggtgta ataaccttcc tcagcggtat aatcgatgat ttttccacca tcgaccaaaa
     4261 gcatcatcat gtttggatag acatggacga ctgttccgtc tgttaaaaca tcttctgttc
     4321 ctttacgatt ggagccacgt ttgtcatctt tgcggacttt gacacctttt gtcatgaccg
     4381 ttgtatcact catattgtct ggttcgatga cttctagcca ttgatctgcc agaccgccgc
     4441 caaccatact tgttgctgct ttgataagac ccataaaaaa ttcctcctct gattcagcta
     4501 gtgccaaaca attattgttt atttattata ccataggaag atgtgaaact tctcggacta
     4561 gagacggaat tgaaatggaa aattgcgtat tgtttaaata cctgaaaaag ccgagaagga
     4621 tagtagtttt tttacctaat atcacgtata ataagagata gtgaattttt taaaaaaggg
     4681 ggatccttat ggcatatcaa gcactttatc gagtctggcg ttcgcagcgt ttcgaagaca
     4741 tcgttggaca aaaagctgtg acacagacac taaaaaatgc tattgtttcc cacaaaacct
     4801 cacatgccta cttgtttaca ggacccagag gaaccggaaa gacgagtgca gcaaagattt
     4861 tcgcaaaagc aatcaactgt ccgaacagta aagacggtga gccatgcaac gaatgcgaaa
     4921 tgtgtcgatc aattactgct ggtacgcaag aagatgtgat cgaaatcgat gctgccagca
     4981 ataacggagt cgaagaaatc cgatttatcc gtgatcgggc aaattatgcg cctacaaagg
     5041 cagcgtacaa agtgtacatt attgatgagg tacatatgct ttcaacagga gctttcaatg
     5101 ccttgttgaa aacattggaa gagccaaaag aaagtgtcat attcatctta gcaacgaccg
     5161 aacctcacaa aataccagct acgatcattt cacggactca gcgatttgat tttaaacgga
     5221 tcaatacggc agatattgtc gaacatcttg aatttatctt gaaagaatcg actatcagct
     5281 atgaaccagc tgcattgaat gtgattgccc gttctgcaga aggcgggatg cgggatgcat
     5341 tgagtattct tgatcaggca atctcgttta gtgaaggaac aattacttta gatgatgcca
     5401 tgcaagtgac aggtagctta actgacgaaa tgatggacag ttacttgagt gcttgcgtga
     5461 aaaaagaagt accagaagca ttggaaacat tgaaccaaat tctcgcagcc ggcaaagaat
     5521 cacgtcgctt tctagaggat atgttacaat actgccgtga cttgctgatg tatcagcaag
     5581 cacctaacct agttgaggaa caatcgagta cgatcagaag tacatttaca gaattggcac
     5641 aaactgtttc agcggatcag ctgttttcaa tgataaagat tttgagcgat actcaaaatg
     5701 aaattcgttt taccaatagt gccgctattt atttggaagt cgctacagtc aaacttgcac
     5761 agctaccgtc tgcttctgtt ccttcagcag atgtagcatt tgcagatgat cagatagtaa
     5821 aacagctaca acaagagatt aaggaactaa gaaacgaaat caaggaacta cgcagtaata
     5881 caggaattga gaatacaaaa gtacagccgg tacagcaaac aaatcataaa aaacagacaa
     5941 gtacctatcg tgtaccgacg gaacgagtat ataaagttct gcgggaagcg acaagacagc
     6001 atctgaacca agtaaaagaa gtatgggaag acttgctgat gagtctatct gtcactcagc
     6061 gagcaatgct taaagcaagc gaaccagttg cagctggacc tacaggacta gtgattgcgt
     6121 ttgattatga gattgtttgt caaagagcag caaatgacga agaattgcag ctttcggtac
     6181 acaacaactt aagccgaatg atcaaagatt atgcgcctga tcctgtttat attacaagag
     6241 aaagctggcc agaacttcga agaaattatt tgacgcaggc acaagaagag ggaacagaaa
     6301 actcttttgc ttctgaagac tcagaaggag aaatcgagtt gcttcctcaa gaacaagaag
     6361 taccagaaga aacacttgta gtaaaagcag aggaactgtt tggctccgaa gctgttacag
     6421 tggtagatga ttaataaaaa ggagtagata attatgatgc gtggaatggg caatatgcaa
     6481 ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg cagaagcaca agagctgcta
     6541 aataaaacag agtttacagg taaagccacc aatgaattgg tcaaagcggt ttttacaggc
     6601 gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg ttgatcctga tgatatcgag
     6661 atgcttcagg atcttgtcgt aatggcagtc aatgatgcga tcgcacaaat tgatgtggaa
     6721 actgagaaaa aattagggaa atacgcaaaa ggtttgccag gattttagtt ttaaactgaa
     6781 ggagtaatga atatgcaata tccagaacca attgcgaaac tgatcgacag ctacatgaaa
     6841 cttccaggaa tcggtcagaa aacagcgaca agactcgcct tttatacgat cgatatgaaa
     6901 gatgaagtag taaatgaatt tgctaaatct ttgttaagtg taaaacgcga tttgcatttt
     6961 tgtagtatct gcggaaacat cacagaagaa gatccctgcg aaatatgtag aaatccttca
     7021 cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag atgtaatggc aatggaaaaa
     7081 atgcgagaat atcacggact ttatcatgtt ctccatggtg tgttatcacc gatggaaggg
     7141 acaggtccag aagacatcaa tatttcttct ctgttgcagc ggctgcatga tgcagatgta
     7201 aaagaagtga ttatcgcaac aaatgcaaca acagagggag aagcaacagc tatgtatctc
     7261 tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc tagcacatgg tctgtctgta
     7321 ggctctgata tcgagtatgc agatgaagtt actttattga aagctgtcga aggaagacgt
     7381 gagttataaa tttctaatta agtgtttttt cagatacaat ataaaagcag cacaagagat
     7441 gggggcaaca atgtgaacgg actatttatt acaatcgagg ggcctgatgg tgcaggaaaa
     7501 accagcatct tgaatgaact ttaccctctt ttgaaagaag tagcaaaaac agaaatcatt
     7561 caaacaagag aaccaggagg cattccgatt gctgaagaga tccgagcagt gatccttgat
     7621 ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc tttatgcagc agcaagaaga
     7681 caacatctag tcgaaaaggt tctgcctgct ctggcaaaag ggaaaatcgt tttatgcgat
     7741 agatttgtcg acagttctct tgcttatcaa ggtgcaggcc gacgcatcgg cgttaccgag
     7801 attgctcggt tgaatgagtt tgctacagaa ggtactacgc cggattttac cctttatctt
     7861 gacgtagatt cagacaccgg attaagaaga atcaagaaaa acagacaaaa tcaaatcgac
     7921 cgtttagatt cggaaggttt ggaattccac cagcgtgtac gtcatgctta cttaaagctg
     7981 gcagaagaga atccggaaag aatccacaaa gttgatgcaa gaaaaagctt tgaagaagtg
     8041 ctgcaaacaa gttatcatac aattattgaa cagtatccgc aattttttga aaactaggaa
     8101 ggtgaacgtt tatgaaaatt attttagcga ttatccaaga caaagacagc aaccgtttat
     8161 caaatgaatt gattgatgca aatatccgtg ccacaaaact atcatcgaca ggcggcttcc
     8221 ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga tgaccgtgta gaagaagcat
     8281 tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt tgtatctaca cctgttacac
     8341 tggatatttc gatggatggg ggcgtccctt atccagtaga agtagaagta ggcggtgcca
     8401 cagtcttcgt cttacctgtt gaaggattcc atcaatttta ggaggagctg agctatgaat
     8461 caagaaataa acttggctca aatgcaaccg atcgtgtatc agcagcttca acggagtttt
     8521 gagcatgggc gtatcgctca tgcttatctt tttgaagggg agaaaggaac aggaaaacat
     8581 gaagtaggta tatggcttgc acagcacctg ttctgtaccc aaatgaaaga caatctgcct
     8641 tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag aacatccaga tgttttggtt
     8701 attgaaccgg aaggacaaac catcaaagtc gatcagatca gacgtttgca gacggaattc
     8761 agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga tcaaagaagc tgaaaaaatg
     8821 aatagcagtg cggcaaacag tttgttgaaa ttcttggaag agccccctgg agatttttta
     8881 gcggtgttag aaacagatgc agtaggaaga attctcccta ctattcaatc tcgttgtcaa
     8941 gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc atcgattgca agaagagcag
     9001 attccattgg aaaaagcaaa acttttggct ttcttaacaa acagtttagg aaaagcagtt
     9061 gaaatctctc aagatgaatg gtttaatgat gctaaggact tgatttatca atggttcgtt
     9121 tacttgcaaa agcaagatac tcaagccttt atctatgtcc aaaaaaaatt ggtaaaatca
     9181 tttaaagaaa aaacacaaca gtttacagga ttatctgtct tgatgtttta ttatcaggaa
     9241 gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag tgaaacagat aaaccagata
     9301 attgaacgga tattgatcgc agagcagaaa ctacgcagca atgtcagttt tcaggctgtt
     9361 gcggaacagt tcgttttgca tacgattttt tagcttgccg caaaggatga tgatcaagga
     9421 ggatggaaat ggtagaagta gtaggtgtcc gatatagaga tgcaggtcat atctattatt
     9481 ttgcaccagg agactctgaa tatgtttata gtgacaaggt attagtcgaa tctcagcaat
     9541 ccttacatat tgctactgtt gtccttccta aaaaagagat tgatcccaat gaccttcctg
     9601 aagctatccg tccgatcttg cataaagcgt cacaagctga attagaaaaa gacaaaaaaa
     9661 acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga aaaagtgcag ttgcgtgagt
     9721 tggaaatgaa aatggtcaaa gccgaataca catttgatcg aaataaattg attttttctt
     9781 tcacagcgga cgggcgaatt gactttagag aattggtaaa agatctggcc gctgtatttc
     9841 gtactcgtat tgagcttcga caaattggtg tccgcgatga agcaaagata ataggcggaa
     9901 ttggtccatg tgggcgtcca ttatgctgtt cttctttttt aggcgatttc gtgcctgtat
     9961 caatcaaaat ggcaaaagat caaagtttat ctttgaatcc aacaaaaatt tccggtttat
    10021 gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga atacgagaca gcgaaaaaag
    10081 aactccctga ctttggaaaa gtaattgcta cacctgatgg taaagggaaa gtcgtagggt
    10141 tgaatctgct tagccgtatc gttagtattc ggctgactgg acgggaaaat cctgtcgaat
    10201 atgaatggga tgaattgaaa gaatttatcc aagtaggtga gatgaatgga taagagatca
    10261 ttatacgacg gattgaatca attagaatca gagttgagaa atacattaac agaacttgtt
    10321 gaaatgaagc atgctttgca tgagatcgta gaaaaaaata caactttgga gatggaaaat
    10381 caacgtctgc gggaacacct gcgggaattg aaccaagttg cacaagctcc agctgaaaat
    10441 gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa aaatttatga agaaggattc
    10501 catgtctgct acgatttata tggatcaaga agggacaatg acgagccttg tgcgttttgt
    10561 ctagaagtga tctatcgcga aagcggtcga tgaccaataa caagaggaga ctggagcgga
    10621 gaaatactgc tccagtcttt ttattaagga gattgtgaca gatgtataat caaaaaagtt
    10681 tcaagggaaa tccggtatat ggggatttat atcttgtacc gacgccaatt ggaaatttag
    10741 aagatatgag ctacagaagt gttcgtatgc tgcaagaatc cgatcttatc gcaagtgagg
    10801 atacgcgtaa tacacaaaag ttattgaatc attttgaaat ccatacaccc caaaaaagtt
    10861 tacatgaaca taattataaa gaacgtgtcc ctcaattgat tgctttgtta aaagaaggaa
    10921 aaacgattgc tcaagtcagt gacgcaggaa tgccttccat cagcgatcct ggacatgagc
    10981 tagttttagc ctgcataaaa gaagggattt ctgtgatctc tatcccagga cctacagctg
    11041 ggatgacggc tttgattgct tcagggctag tgcctcaacc atttttattt tatggcttcc
    11101 ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa gctaaaagaa cagacagtta
    11161 cgctgatttt ttatgagtca ccttatcgga tcactgctac attgacgaat ttcttagaaa
    11221 tttttggaaa tcgccaagtc gttttgtgtc gggaattgac aaaggtccat gaagaatacc
    11281 ttagaggaag tacggaagag ctactcgctt atatcgaaga acatccagta aagggtgaat
    11341 gttgtctgct tgtagaaggg aatttgttcg aaatgccagt gacggaagtt gatgaagagt
    11401 tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc aggcgaaaaa ccaaatgctg
    11461 ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca agaagtctac cgtcagtacc
    11521 atgaattaga ctgagatagt ataagttgtc tataatgaaa gactaatgag gaaatcagct
    11581 actaactttt caaatctctt gaacgcgaac gaatgttctt gtataatgaa taagaggtga
    11641 ggatgatgga gctgcaattt cctttacata atgatacatc aagaaaaatc attcatattg
    11701 atatggatgc ttttttcgct tcggttgaag aacgagatca tcctgagttt cgcggacatc
    11761 cattagtaat tgccagacac cccagtgaca ctggtgggcg tggagtagtg actacagcaa
    11821 attatgaagc aagaaaattc ggaattcatt cagcaatgag tgcacaaaaa gcgtatgagc
    11881 tttgtccgca agccatcttt aagcctggcg accatcataa atacagcgat atttccaaac
    11941 aagtacggga agtattctat cgttatacag atattgtgga accagttagt atcgatgaag
    12001 cgtatcttga tgtgaccgaa aataaaatca attgcaagtc agccatcaaa atcgcccgaa
    12061 tgatccaagc agatatttgg gagagtgtcc agctaacatg ttctgcggga gtaagttaca
    12121 ataagttttt ggccaaatta gcatcagact atcaaaaacc tagaggaatc acggtagttg
    12181 cccctcaaga cgctgtgcca tttttaaaag ctttgccaat tgaaaaattt catggggtag
    12241 ggaagaaaac tgtacctaga atgcatgagc tagggattta tacaggaaaa gacctatatg
    12301 aatgcacaga aatgatgttg attcgaaatt ttggaaaaat gggctattct ctttatcgta
    12361 aagtcagagg aatccatgat tctccagtaa acgttacaag agagcgaaaa tctgtgggaa
    12421 aagagcatac ttatgggcag ccgttgcaaa cggaagaagc agtgctgact cagttgcgcc
    12481 aactagctga aaaagtagaa gaagcactga gaagagtcca aaaacatggg aaaacagtag
    12541 ttttgaaggt caggtacacg gactattcta ctgtgaccaa aagagtgaca ttgccagaat
    12601 atatctataa aaaagaagca ctcttttatc aagcaagcct tatatgggag gaaatcttgg
    12661 gagtagaaaa aggaatccga ttgttaggga ttactttgac gaatctggac ccgatgactt
    12721 atgaaaacat cgtccttcca ttatgggaaa atcaagaaat ataaaaacag ctttgagagt
    12781 tgaaaccgga aatccgtttt ctctcccaaa gctgttttta tatcaagtct tgctttttga
    12841 tttgttcata ggtttctgtt gcatccgtac atttttccca aatcacaact tgattggatt
    12901 ctaaagattt ttggacgtca atgatccgct ggttgctact tccgcgaaat tgtagtttta
    12961 ggtcacgttt gcttaattca aagcgtccgt caacaaggat atcgatttgc gaaaggagtt
    13021 ctaatttatc ttctgaatct tgtagcaatt catcaaaggt atagcctgtc catgaccaga
    13081 tatcttttgt cgtaccgaac tcttttcgga tacgtcgtac gacttgcaga caaacatctg
    13141 tattcaaaaa aggttcgcca ccaagtaaag ttaatccttg cacgtaatcg ttgcgcaagt
    13201 cagcgatgat ttgttcttct aattcaggtg tgaatggttt gccatatcgg aaattttgtg
    13261 cagctttgtt gaagcagcct tcgcaggcaa ataagcagcc actaacgtaa agactgttgc
    13321 ggacaccttc accatcgaca aaattaaacg gtttgtagtc agcaatatag ttttggctgt
    13381 agtcttttgc taaccattct tttggttttg gatttctcat aaaaaaactt ctttcattta
    13441 aggttcaaag atatagctat tgaagataaa atctactcga ccacataaat aagaacaaac
    13501 tgacctaaat ccattgttca agaaagcttg ttcttatttt gatagagggc gttcaaagat
    13561 acaaagccat cgaaagaggg tggtgaaaaa agacttgtca tagcctctta ttctgaataa
    13621 acggtgttca aaagcttgca tctgcagtaa taagcccaac taaacgaaaa atatgaggaa
    13681 ctatttttgt ttagctattc ttattacttg atgcaggaca gctttttcac aacctcattt
    13741 catgtgtttc acacgtgagg aaatttcttt atggcggcca tgtaccatcg gacgagcttg
    13801 cggatttcct aaatagccac aagtacgttt gactacatca catgtttttg gatcatgatt
    13861 tccacattct ggacattcga accctcgttc tgtcggtttg aaatcacctt cgaaaccaca
    13921 ttgatagcaa tgatcgattg gcgtattcgt accaagatac ccgattttgt catatgcaaa
    13981 atcccataca gcttctaaag ctttcggatt ttgttggagc atagggtatt cacagtagtg
    14041 gatgaatcca cctgaacaat attttggata atccttctca aattccaatt tttcaaatgg
    14101 cgttgggttt ttacgcacat catagtggaa actgtttgta taatattctt tatctgtgat
    14161 attttcgatg atgccgaatt tttctgtatc caaacggcag aaacggtcag ttagactttc
    14221 acttggagtt gaatagacac tgaaatgata accatattca tccccccatg catctgcatt
    14281 agcttttaat tctttgacga tatcaagtgt gaaagctttt gcttctgggt ttgtttccca
    14341 ttcgccaccg aagaaagctg aagcaacttc atacaatcca atgtagccta acgatacagt
    14401 agctcgacgg tttttgaata attcattgac agaatcggta cgtgaaagac gtttcccgaa
    14461 agcaccatac atatataaaa ttggcgcatt tgctggaatg gcttctttac aacgttctac
    14521 acgatagaga agcgcatctt tcatgatttc taagcggtca gacagtaact gccagaattt
    14581 attctgatct ccttttgatt cgatcgcgat tcttggcaaa ttcaatgtaa caacacctaa
    14641 attcattcgt ccgacattta cttcttgacc attttcgtct ttccaccctt gcaagaatga
    14701 acgacagccc ataggtactt tgaaacttcc agttaattca acgattttat cgtaattcag
    14761 aatatcagga tacattcttt tcgttgcaca ttccaatgct agttgtttga tgtcgtaatt
    14821 aggatcttca gggtttaagt tcacaccttt tttcaaagtg aagatcagtt tagggaagat
    14881 agctgtccgc ttttcactcc ctaatccatt gattcgaatc tgtaaaattg ctttttgaat
    14941 ttctctttca aaccagtttt ctcctagtcc aaatcctaat gaagtgaaag gagtttgtcc
    15001 atttgaagta aataaggtat tgatttcata ttccaaactt tgcatagcat cgaagatatc
    15061 ttttttggtt tttgcttttg catattcttt ttggcgttct ggcgaatcga tccattcttc
    15121 cgcgtctttt agatgttttt tatagttcaa ttctgcaaaa ggagcaagca attcatctgt
    15181 tcggtcggca gaacagccac catattgact tgacgctaca ttggcaataa tctgtgagat
    15241 ttgtgctgtg gcggtctgga tagattttgg cgattcgact tcggcattgc cgattttgaa
    15301 cccattgttc agcatgcctt tgaaatcaat caagcagcaa ttcgtcatcg gtgtataagg
    15361 atgataatcc aaatcatggt aatggatatc gcctttttga tgcgcatttg ctatatgtgg
    15421 tggcaacatt ttcagtccga ttgattttcc aacgatcccg gctgtcagat ctcgctgtgt
    15481 attgaaaaca ttactgtctt tatttgcgtt ttcgttcacg acagtttggt ccttgttgat
    15541 tagcttttca atcgaaaagt tgatatctgt tgctttactg cgagcaaaat ctcgtttggt
    15601 acgataattg atgtactctt ctgctaattc atattcgttt ttttctagta atatatgttc
    15661 cacgatgttt tgaatctcat agattttgac attttcagca aaccggcttt tgatttcttg
    15721 gacaaccaag tctgcaatca tttgaatttt ttcataagta agtggtgata atgagccgtg
    15781 aattttcttt tctgctttga tcagtgcttc attgatcttt tgttcatcga aatcaacatg
    15841 gcggccatcg cgtttgatga ctttcatctg ggatgtctct ggctgctgag taacgacagt
    15901 tttttcttca taatttactt tcattatctt caactcctgc taaattgttc ttgtctatca
    15961 taaaacaaaa tcggggaagg agcaactaaa tatcggctat atatagtatt c
//
LOCUS       DAHEYX010000051        15807 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHEYX010000051 DAHEYX010000000
VERSION     DAHEYX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.052"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(70..1740)
                     /locus_tag="MB219_001101"
     CDS             complement(70..1740)
                     /locus_tag="MB219_001101"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCC8426830.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGIIAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNEWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            1922..2155
                     /locus_tag="MB219_001102"
     CDS             1922..2155
                     /locus_tag="MB219_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCC8426831.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(2254..2685)
                     /locus_tag="MB219_001103"
     CDS             complement(2254..2685)
                     /locus_tag="MB219_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCC8426832.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            2764..3177
                     /locus_tag="MB219_001104"
     CDS             2764..3177
                     /locus_tag="MB219_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCC8426833.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(3215..4363)
                     /locus_tag="MB219_001105"
     CDS             complement(3215..4363)
                     /locus_tag="MB219_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCC8426834.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFDDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(4485..5081)
                     /locus_tag="MB219_001106"
     CDS             complement(4485..5081)
                     /locus_tag="MB219_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCC8426835.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(5281..7923)
                     /gene="alaS"
                     /locus_tag="MB219_001107"
     CDS             complement(5281..7923)
                     /gene="alaS"
                     /locus_tag="MB219_001107"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCC8426836.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            8311..8742
                     /locus_tag="MB219_001108"
     CDS             8311..8742
                     /locus_tag="MB219_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8426837.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLA"
     gene            complement(8787..10136)
                     /locus_tag="MB219_001109"
     CDS             complement(8787..10136)
                     /locus_tag="MB219_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCC8426838.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(10206..11369)
                     /locus_tag="MB219_001110"
     CDS             complement(10206..11369)
                     /locus_tag="MB219_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCC8426839.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKLAFDSGTVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            11498..11821
                     /locus_tag="MB219_001111"
     CDS             11498..11821
                     /locus_tag="MB219_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426840.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPQALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(12003..13352)
                     /locus_tag="MB219_001112"
     CDS             complement(12003..13352)
                     /locus_tag="MB219_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8426841.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETRGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13421..14374)
                     /locus_tag="MB219_001113"
     CDS             complement(13421..14374)
                     /locus_tag="MB219_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCC8426842.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWDLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDAMTKDMLEKLKEKLVETV"
     gene            complement(14476..15645)
                     /locus_tag="MB219_001114"
     CDS             complement(14476..15645)
                     /locus_tag="MB219_001114"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCC8426843.1"
                     /translation="MYLEKELDQLKEKGLYNTIDILESENGACIIVDGKRMINLASNN
                     YLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGTEAAIAFQ
                     SGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKDLEKKAKE
                     AIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLVTYVDDAHGSGVTGKGAGT
                     VKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSLTPGAAAA
                     ALASITLMQEHPELVEKVWENANYFKEELKKAGYNIGMSETPITPVILGDEKVTQTFS
                     KKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKEMEII"
BASE COUNT         4574 a         3338 c         2751 g         5144 t
ORIGIN      
        1 tgtagtcaaa atacagctcc ttcgatgttt aatgaaagtt atttagtata cggtagatta
       61 taatcaagct taaaacaatc ccaacacttc tccgtcattt gtcacgtcca tattcaatgc
      121 agcaggtttt ttaggcaaac caggcatcgt cataacatct ccagtcaagg ctactaaaaa
      181 tcctgcccct aattttggga tgatttcgcg gatcgtgatg gtgaaatctt ccggtgcacc
      241 tagcagtgaa ggatcatcag aaaatgaata ctgtgttttt gccatacaaa tcggcagttt
      301 gtcccattca ttcttcgtaa actcttctat ttgtttttgc gcttttttgc tgaaaacaac
      361 accatttcct ccgtaaattt tcttgacgat tgtttctgtc ttttcttgta tcgtttcttc
      421 taggcggtat aatggcttgt aattagcttc ttttctatca atcaatcgta ctacagcttt
      481 tgctaaatca acgccgcctt ctgctccatc tgcccataca ctcgctctct tggcaataat
      541 cccctgattt tcacacaagt gctccaacag tgtgagttca ttgtctgtat ctgttacgaa
      601 ttcattgatt gcgacgatca caggtagctg atattgttcc atattacgta tatggcgctt
      661 taaatttgca aaacctactt tcaaagcatc aagattctca tttttaagtt catctttttt
      721 cattccgcca tgcattttca atgcacggac agtggcgaca atcaccacag catccggcgc
      781 ttttttcaag ttaggtactt tgatatctaa aaatttctct ccgccaagat ctgcaccaaa
      841 tcctgcctct gtgacaacgt aatctgctaa tctcaatgct gtcttggtag ctaaaatact
      901 attgcagcca tgagcgatat tggcaaacgg tcccccgtgg acaaaagctg gtgttccgta
      961 aattgtttgt acaagattcg gcttgattgc atctttcagc agtaatgcca gagcgccttc
     1021 tgctttcaag tctccagcag tcacaggctg acggtcaaat gtatagccga caacgatcct
     1081 tgcaagacga gcctttaaat cttcaaggtc tgccgcaaga cacaagatag ccatgatttc
     1141 gctggcaact gtgatatcaa acccatcttc tcttggaaca ccttggattg gcccacccaa
     1201 gccaacaatt actttacgca gttcacgatc attgagatcg actacccgtt tccaaatcac
     1261 tcggcgggca tcgatgttca attcatttcc ttgatgaata tgattatcta aaagagcaga
     1321 taatgcatta ttagctgttg tgatagcatg catgtcacca gtaaaatgca agttgatatc
     1381 ttccataggc aatacctgtg catatcctcc gcctgcagca ccacctttga ttcccatcac
     1441 aggaccaagt gagggttctc gcaatgcaat aacagatttt ttattgattt tattgagcgc
     1501 gtctcctaac cctacagtga tcgttgactt tccttcgcca gctggtgttg gattgatgga
     1561 agtaactaag atcaaatgcc cctcagcgtt atcctttgat cgattgattc cagaaaaatt
     1621 gattttcgcc ttatatttcc catacagttc caaatcgtct tccttaagat ctaatttact
     1681 agccacttca acaatcggtt ctagctctgt ttcttgtgaa atttgcagat cattcttcat
     1741 ttgattaccc cttttcacga actttattca acaaaatatc ttctttgtta agatatgcca
     1801 attatacctt tttttataat aaaaaaaaca taaaattaca ggtttttaat ttatttgacg
     1861 tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg tcgtgagtgc
     1921 tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct ttatcagtta
     1981 tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg gattcaaaac
     2041 tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag gaacacaagc
     2101 tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg aatagataaa
     2161 caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc ttttcctgta
     2221 aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt tcgtataagg
     2281 ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa ggactacttg
     2341 atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca tcatttgcac
     2401 cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc ggtctttcca
     2461 aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac gtcgcaaacc
     2521 tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat aaggacctgt
     2581 gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg accaatagat
     2641 gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct aacctctcac
     2701 cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg agaatctctt
     2761 tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc tagtggaggc
     2821 ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct ttctataggg
     2881 acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct aaagaaaaac
     2941 gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt ttctacggtc
     3001 gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat ccaagaactt
     3061 ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa taaaggagca
     3121 gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac tctttaaatt
     3181 aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa ttgtgatcaa
     3241 acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt aagattcttt
     3301 ctagttctgt atcatcaaac tggaatttct cagggaccac tacatcaaat tgcagcgttt
     3361 ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt tcaagaagaa
     3421 tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc attggatcca
     3481 agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc agatcaataa
     3541 tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact gatgcaaagc
     3601 gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata tcatcgcaag
     3661 catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt cctaatagtt
     3721 cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat gctaaagcaa
     3781 aacctatata tccatcgata cgccatccag ttacccattc gattgctgct gaaagcaata
     3841 cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc aatgtttcag
     3901 aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc aaaaagatgg
     3961 aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga tggatgattt
     4021 tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc gaaacaaata
     4081 acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct gctggctttg
     4141 cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat agactgttca
     4201 ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc ttagagacaa
     4261 acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt tttgtccgct
     4321 gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt accccttcta
     4381 gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct cctctcttcc
     4441 tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc tggtttcaac
     4501 ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc acctattgct
     4561 tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag agcagaatct
     4621 acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc taacactaag
     4681 actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt tattgattta
     4741 tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag tactctggga
     4801 actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt tggcatggca
     4861 taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc atcttttggg
     4921 cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact aatgaatcct
     4981 aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc taacaagatc
     5041 gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc cctttttatt
     5101 tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg aatccgacca
     5161 tttgattaga tgcgccagcc aattgaaaaa agtatgaggg caagacttgt ttgtcttcgc
     5221 ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa cgtaaacgcc
     5281 ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac cagcaggatt
     5341 tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta ctttcggtgc
     5401 gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt tcgtcattgc
     5461 cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat ctgaaatatt
     5521 tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca cttttacttg
     5581 tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat caccagcttg
     5641 ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc ttacttgctg
     5701 ttgcaattgt tccgcttttg aaacaacttc cttcaattga ggtgatttaa caattgttgc
     5761 tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt tacttgttac
     5821 agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt tgaagattcc
     5881 gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc agccgccaat
     5941 atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg cacccatgtt
     6001 tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc agattttttc
     6061 gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa agtgggtaaa
     6121 gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat gctcgccaag
     6181 tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga tgatccgatt
     6241 gcgcatcgat tgatcaatca atagatgata ggtttgtcct tcactcattg gtgcttttac
     6301 ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa caactgctac
     6361 gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac ccatttctgc
     6421 atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt cattcaaaag
     6481 agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg tgtatccaat
     6541 gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca ttgatttttc
     6601 agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt caaaaccagc
     6661 atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta ccgggaagcc
     6721 atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt tttcgttttt
     6781 tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt cattgaaacg
     6841 ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta cttcaggata
     6901 ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg cttgttcaat
     6961 acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga cataccctct
     7021 cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc gaatatgatc
     7081 tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta cttgtttgct
     7141 tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt tcgttggcgc
     7201 atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt ttttatgagg
     7261 aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta agttccagat
     7321 ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt ctgctaaatc
     7381 gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg ggcctgcacc
     7441 gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc caacttcttc
     7501 atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa gtttttctgg
     7561 atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga tggcttcatt
     7621 tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat ggtgccgtgc
     7681 tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat ttgtgattct
     7741 tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa caccagagtt
     7801 gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt caatcgtatg
     7861 tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac ttaattgttt
     7921 catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca gccaaggacg
     7981 aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt aaactcgata
     8041 acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt gattagtgac
     8101 tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa tcatatcctt
     8161 ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa tttttatttt
     8221 catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg aatcaataca
     8281 tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc ggatgaatgg
     8341 atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca agggattcct
     8401 gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct atttcttgat
     8461 caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg cttcaagccg
     8521 gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca gttgcttgaa
     8581 gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt agctgaaatg
     8641 acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc ttttattgaa
     8701 gatgaagtac tttgtatcgg aatggaaaaa agtctagctt aaaaacaaga gattgtgaca
     8761 cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt tttacgagca
     8821 gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc tgcaattgcc
     8881 caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt cacaagacca
     8941 atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct attacgatcg
     9001 tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac gcccaatttt
     9061 tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc cgttcctttt
     9121 aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc ttctggaatt
     9181 tctgcattga taacatgaga aacaccttcg atatcgatcc ctctagcagc taaatcagtc
     9241 gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt tctctctctt
     9301 ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa gtaatcggca
     9361 atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg atgaccaatt
     9421 gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat cagccaattt
     9481 tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg attttccatg
     9541 tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac taaaaactgc
     9601 agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc catatccaat
     9661 gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag cgcttgttca
     9721 ttcatcatgt ctaatattct accaggcgtg cctactacga cctgtggctg ttggtgatgc
     9781 agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac acggatctcc
     9841 ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc tagttcccta
     9901 cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt ttcaaacaaa
     9961 ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat gatatttttt
    10021 ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt gaagcccttt
    10081 gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt attcattcgt
    10141 gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa agggtttttg
    10201 ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac agtatatgtg
    10261 tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag caagatattt
    10321 aaaataaaaa taccttgcag taacaagaca ccgagagaaa tgaatactgc actcatctgc
    10381 aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa ccagcttaaa
    10441 ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa agaaattcgt
    10501 tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt ccatccattg
    10561 gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca ccagatatga
    10621 gaagtaactg tccctgaatc gaacgcaagt ttaaagaaaa tcgccgctac caaattaata
    10681 agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa tgagacagtc
    10741 tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat aaatgtaaat
    10801 aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa atcattcaca
    10861 tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg cgtcaattca
    10921 aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag ccggaaataa
    10981 tatcttaaat aaatgccaaa tggtatgagt aataaaacat taaaagctgg ttccaaaaat
    11041 acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa taccgtgttg
    11101 tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc agtcatttgg
    11161 gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca caataaataa
    11221 aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata ctttcgatat
    11281 tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac aatcagcgca
    11341 aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt gtatctctct
    11401 tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc tcatttcttt
    11461 caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt actttgtttt
    11521 tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa gaaaaagaac
    11581 tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc tagtcttcaa
    11641 tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac aagctttgtc
    11701 taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt ctggttgctt
    11761 ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca aatcaaagta
    11821 aaaggcagaa atcggttgag cccgatttct gtcttttact ttgctatttc attgtagaca
    11881 aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc acgtactcct
    11941 catttaaata aaatattttg tttggcagag agtgcagttc taataaaatt cgaatgtttt
    12001 agctatctac gttttaaata gagataaatc gggataccgg caagtgtcaa tactatcccc
    12061 atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt ccctccaatg
    12121 attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa caaatcaggt
    12181 tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat ccaaatgaca
    12241 aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc aatagcgatg
    12301 actagctcta agatacctgc ggctacagga actttagttt tatcatgaag tttagcaaag
    12361 agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg cattccagta
    12421 agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt gaccagtctt
    12481 ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct gtttcctgcg
    12541 actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata aacgatcatg
    12601 attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt tttcaattct
    12661 cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc tagcaagccc
    12721 gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa cagttggaag
    12781 tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg gataagcttg
    12841 cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa attgatcagc
    12901 aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga aagaccaaat
    12961 agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc tggaaaataa
    13021 atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat ccgttcgata
    13081 tatttaatca tccctcttgt ttctggaata gcagctgcca gttcagctcc tgttagtcct
    13141 gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact ggcggatcct
    13201 gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac tgtgcccata
    13261 acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc tatttttact
    13321 tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata ccatagccaa
    13381 tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc tctaccaatt
    13441 tttctttcag tttttccaac atatcctttg tcatcgcatc taagtcgtat ttcggcgaaa
    13501 agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat tctgctattg
    13561 actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt ttttgaatag
    13621 atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca tttcgatgaa
    13681 ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca tctggcatat
    13741 acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct tctttgattg
    13801 ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag gttgcataag
    13861 aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca taataataat
    13921 cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga cgctgaagag
    13981 tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt gaacttggcg
    14041 taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc ccagtcacgt
    14101 tcaaatccca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga gcagccaaat
    14161 gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg taatccatta
    14221 catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt atatctgtcg
    14281 caatgacatt ttcactgcct agttcttgac gtagtcgtgt gatcaactct gaaccaattt
    14341 gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat cttttttact
    14401 actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt aaatagacga
    14461 aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat aaactgctac
    14521 tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga tcctgcctgt
    14581 cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt cgatcagctt
    14641 cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa taggtgtttc
    14701 agacataccg atgttatagc ctgctttttt caattcttct ttaaaataat tagcattttc
    14761 ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag ccaatgcagc
    14821 tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc tagctttcaa
    14881 ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta tagctttcga
    14941 taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga cggttcctgc
    15001 accttttcca gttacgcctg agccgtgcgc atcatcaacg taggtgacca gtccatattt
    15061 ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg agaaaacacc
    15121 atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct ttgctttttt
    15181 ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg ataagcggca
    15241 accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt cttttgtcat
    15301 tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga ttgctgcttc
    15361 tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca gcgaaccatt
    15421 gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa tacatgcttt
    15481 tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat tgatcatcct
    15541 ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga tcgtattgta
    15601 taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta gttcttttgg
    15661 aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa catatatcgt
    15721 gtcattcatc tacaaagcaa gtatgccata tttttgataa cgcttacact gattatgaga
    15781 attatttaac cacatttttt atttaca
//
LOCUS       DAHEYX010000052        15136 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHEYX010000052 DAHEYX010000000
VERSION     DAHEYX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.053"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="MB219_001115"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="MB219_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCC8426844.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="MB219_001116"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="MB219_001116"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCC8426845.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="MB219_001117"
     CDS             1947..2645
                     /locus_tag="MB219_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCC8426846.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="MB219_001118"
     CDS             complement(2813..5452)
                     /locus_tag="MB219_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCC8426847.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="MB219_001119"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="MB219_001119"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCC8426848.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="MB219_001120"
     CDS             7295..8470
                     /locus_tag="MB219_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCC8426849.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="MB219_001121"
     CDS             8474..9115
                     /locus_tag="MB219_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426850.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="MB219_001122"
     CDS             9112..10029
                     /locus_tag="MB219_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8426851.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="MB219_001123"
     CDS             10026..10697
                     /locus_tag="MB219_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426852.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="MB219_001124"
     CDS             complement(11238..11726)
                     /locus_tag="MB219_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCC8426853.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="MB219_001125"
     CDS             complement(11927..12193)
                     /locus_tag="MB219_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCC8426854.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="MB219_001126"
     CDS             complement(12193..12456)
                     /locus_tag="MB219_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCC8426855.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12932..13117
                     /locus_tag="MB219_001127"
     CDS             12932..13117
                     /locus_tag="MB219_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCC8426856.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13259..15082)
                     /locus_tag="MB219_001128"
     CDS             complement(13259..15082)
                     /locus_tag="MB219_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCC8426857.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4385 a         3245 c         2854 g         4652 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggcaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aattta
//
LOCUS       DAHEYX010000053        14635 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHEYX010000053 DAHEYX010000000
VERSION     DAHEYX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.054"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            167..394
                     /locus_tag="MB219_001129"
     CDS             167..394
                     /locus_tag="MB219_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCC8426858.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            410..1576
                     /locus_tag="MB219_001130"
     CDS             410..1576
                     /locus_tag="MB219_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCC8426859.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1811..2854
                     /gene="hrcA"
                     /locus_tag="MB219_001131"
     CDS             1811..2854
                     /gene="hrcA"
                     /locus_tag="MB219_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCC8426860.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2948..3511
                     /gene="grpE"
                     /locus_tag="MB219_001132"
     CDS             2948..3511
                     /gene="grpE"
                     /locus_tag="MB219_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCC8426861.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3562..5391
                     /gene="dnaK"
                     /locus_tag="MB219_001133"
     CDS             3562..5391
                     /gene="dnaK"
                     /locus_tag="MB219_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCC8426862.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5542..6708
                     /gene="dnaJ"
                     /locus_tag="MB219_001134"
     CDS             5542..6708
                     /gene="dnaJ"
                     /locus_tag="MB219_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCC8426863.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7076..7813)
                     /locus_tag="MB219_001135"
     CDS             complement(7076..7813)
                     /locus_tag="MB219_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCC8426864.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7815..9317)
                     /locus_tag="MB219_001136"
     CDS             complement(7815..9317)
                     /locus_tag="MB219_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCC8426865.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9576..11570
                     /gene="uvrB"
                     /locus_tag="MB219_001137"
     CDS             9576..11570
                     /gene="uvrB"
                     /locus_tag="MB219_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCC8426866.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11580..14396
                     /gene="uvrA"
                     /locus_tag="MB219_001138"
     CDS             11580..14396
                     /gene="uvrA"
                     /locus_tag="MB219_001138"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCC8426867.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         4797 a         2589 c         3227 g         4022 t
ORIGIN      
        1 ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc atccaagaag
       61 tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg cgtatatgag
      121 aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga aaatagcttt
      181 tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg
      241 agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt tagagctagg
      301 tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac gcgattttaa
      361 gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa tgatagaaac
      421 aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt gcgagcatat
      481 ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag atgagtatgt
      541 ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag cgaatttgga
      601 aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac ttgatcgact
      661 attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta ctgtagaagc
      721 gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg gcgtcaatcg
      781 tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg gacgtaaaca
      841 tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt ttaccaatgt
      901 aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc gggatacttt
      961 ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga tcctcgaaaa
     1021 caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg atcaggaaac
     1081 ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac gtcaccaata
     1141 tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca tgtactggaa
     1201 caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag atattcgtta
     1261 ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg aacgcccggt
     1321 attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact
     1381 aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa aaacaattga
     1441 atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg ttaatgaaga
     1501 agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt ttgagcgctt
     1561 tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa aagaaaaagt
     1621 ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac atcaattatt
     1681 tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct tgacaggaga
     1741 agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt gctaatgaga
     1801 ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca tccaacacta
     1861 tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag tcaaggccag
     1921 ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc tgttgaagac
     1981 tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg tggatcattt
     2041 gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt ctctaggcag
     2101 agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat ctgagctgac
     2161 aagctatacg actttttcat tgggacctga aataaaagac cgccgactga ctggctttcg
     2221 tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata tggggaatgt
     2281 agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag aaaaaatggt
     2341 acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc ataaacttcg
     2401 tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga tgtctcagtt
     2461 atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg gacaaatgaa
     2521 tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt tttcttttat
     2581 gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata ttcatatcaa
     2641 gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc aagcaagtta
     2701 cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta gtatgcctta
     2761 ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta aacttgctga
     2821 ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct atttgcagct
     2881 gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat gaaaggaaga
     2941 ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg ctcagccaga
     3001 accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga
     3061 attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat acttgcgggc
     3121 tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa
     3181 ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc tggaacgtgc
     3241 attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt
     3301 cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg
     3361 agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga ctgaagatac
     3421 accagccgat acgattgtag aagtactaca agaaggctac aaattacatg atcgcgtgtt
     3481 aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt acactttcaa
     3541 ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg tacgactaac
     3601 tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac
     3661 cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg ggaagttgca
     3721 aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa
     3781 gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat ctcagcaatg
     3841 atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt cgaaaaagca
     3901 gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa agatgctggt
     3961 aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc tgctcttgcc
     4021 tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct tggtggcggt
     4081 acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt gtcaacagct
     4141 ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta catggtagca
     4201 gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt acaacgcttg
     4261 aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac acaaatcagc
     4321 ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac attgacacgt
     4381 gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc agtacgtcaa
     4441 gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt agtcggtgga
     4501 tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac
     4561 aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg tggcgtgatc
     4621 actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt aggtatcgaa
     4681 acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc aacaagtaaa
     4741 tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca tgtattacaa
     4801 ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt gacagatatt
     4861 ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga taaaaacggg
     4921 attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat cacgatcaaa
     4981 tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc agaagcaaac
     5041 gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt agacgcattg
     5101 ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga agaagaagtg
     5161 aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa caacatcgag
     5221 gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac tgttaaacta
     5281 tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca aggcggagca
     5341 gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata atcagttcac
     5401 tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa aatagtttga
     5461 agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg aaaatgatga
     5521 gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt cttagggctc
     5581 tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc gaaaaaatac
     5641 catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt atcagaagca
     5701 tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg gcatgcaggc
     5761 acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt cggcggcttc
     5821 tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg aggaggcggt
     5881 cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac ggtcgacttg
     5941 tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg ggaagaaatc
     6001 tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac ttgtcataaa
     6061 tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg agtgatgagc
     6121 cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc ttgtccaacc
     6181 tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt accagctggt
     6241 gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga aaacggcgga
     6301 ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt tgatcgtgaa
     6361 ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt gggggatgaa
     6421 gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg aacacagaca
     6481 ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg tgcaacaggc
     6541 gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca acaaagagaa
     6601 gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca agaagacggt
     6661 ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt tttctagtaa
     6721 aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa gtcattaata
     6781 attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg tcatgtttgt
     6841 accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa ggtggtatcg
     6901 actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg gaacaggtag
     6961 agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta agtgaaaaaa
     7021 caagaaacta aaaatgcccg gtcgtcatga agcgaccggg catttttggt ttttattaaa
     7081 tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt tcaaagattt
     7141 gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca cgattggcca
     7201 cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct tgttctgcta
     7261 actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca gatgtaggct
     7321 catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc acacgctgct
     7381 tttgtccacc ggaaagcatt tcaggataca tatctttttt atcttctaga ccaactgtct
     7441 gcagcaattt gattgctttt ttctctgctt cttctctaga ttctttcttc aggtccatcg
     7501 gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag tgctggaata
     7561 ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc aagtgggcgc
     7621 cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa cgcaaaaatg
     7681 tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg ttgatggaca
     7741 cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc tcaactaaga
     7801 ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt tgtcaatgct
     7861 gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact ttgtgttgta
     7921 cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc ggaaatgatc
     7981 gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat gatgcgtatc
     8041 gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa acttctggaa
     8101 gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc tgcgatataa
     8161 gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat cgtaaatcca
     8221 acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg aattcctcgg
     8281 atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc tacactgaat
     8341 aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt taggagaatt
     8401 gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc aagaaatgtt
     8461 gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac atattcttta
     8521 ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt gaacatatta
     8581 agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc agattctttt
     8641 ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac cggataatca
     8701 tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc agcagcatca
     8761 aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc agcactttcg
     8821 gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt gatcgaagta
     8881 tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga aaaatcatag
     8941 cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc ttgtcctgat
     9001 tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt aaatccttgc
     9061 agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc ctttgcgttt
     9121 tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc ttttttgggc
     9181 tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt gtcagcttgt
     9241 gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa tgagtgaaat
     9301 aaatgctttc ttttcataat caacctccta aatttccaat attttattat tataggcgaa
     9361 aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg agtttctaac
     9421 attttatgtt ataaaacgta acatgcataa taagataaaa aagtggcgaa agtatgttcg
     9481 gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt attggtcgta
     9541 aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat acctcccgac
     9601 actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg attaaccaat
     9661 tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg actggaacag
     9721 ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca ctaattattg
     9781 cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt ttccctaaca
     9841 atgctgttga atattttgtt tcttattatg attattatca gcctgaagct tatgtgcctt
     9901 caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac aaattacgac
     9961 attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca tccgtttcat
    10021 gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg attcgacagg
    10081 gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa ttcgaaagaa
    10141 atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg gagatttttc
    10201 cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag gtcgaacgta
    10261 tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac gtggcaatct
    10321 tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta gcgaatatca
    10381 aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt ctggaagctc
    10441 aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg ggctacacgt
    10501 cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa ccgccttata
    10561 cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg cacgtaacca
    10621 tgcctcaagt cagaggaatg tataatggtg accgagcgag aaaacagatg ctagtagatt
    10681 acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa gagtttgaaa
    10741 aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa catgaacaaa
    10801 cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca gtgatcgaag
    10861 ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa cgagtcgaaa
    10921 aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac ctcacagatt
    10981 atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa acgctggaac
    11041 gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt ggaatcaatt
    11101 tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg gatgcggata
    11161 aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga gctgctcgga
    11221 atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg cgtctagcga
    11281 tggatgaaac atcaagacgc cgtacgattc agcagaagta caatgaagaa catgggatcg
    11341 ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa gaatcggaag
    11401 atgatacaaa agaagcggta caagtctcgt atgaagaaat gacgaaagaa gaaaaagata
    11461 cgttattgat gaaattagaa aaagaaatga aaaatgcggc gaaagcattg gattttgaaa
    11521 cagccgctaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag gaggacaaca
    11581 tggctaacga taaaattacg attcacggtg cacgtgccca taacttgaaa aatatagatg
    11641 taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct ggaaaaagtt
    11701 ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag agcctttctg
    11761 cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc atcgacggat
    11821 tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg tcgacagtag
    11881 gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta ggacatccga
    11941 tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg gcagatcaag
    12001 taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg gtaaagaaaa
    12061 aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc cgcgttcgag
    12121 tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac aaaaaacatg
    12181 atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct cgtctatttg
    12241 attctttaga ggcggctctg cgattggcag acggctatgc tctggttgac gtgatcggtg
    12301 aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt actgttggcg
    12361 aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca gactgtgatg
    12421 gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga acgaagacac
    12481 ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat cctcaaatgt
    12541 tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa gaattaccgg
    12601 aagagcatca ggatatcatc ttgaatggga cagatgatct tttccatttc cattatgaaa
    12661 atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg aagaatatcg
    12721 agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt ctttatatga
    12781 cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct ctatctgttc
    12841 aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa gcagtagatt
    12901 tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg attttaaaag
    12961 aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta actttgagtc
    13021 gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct acacaaattg
    13081 gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga ttgcatcaac
    13141 gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc aacaccctcg
    13201 ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatctgatt gacgtcggac
    13261 ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa gaagtagcta
    13321 atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc cctgtaccaa
    13381 aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca gaaaacaatc
    13441 tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca ggtgtgtctg
    13501 gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg caaaaattaa
    13561 ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa agtatcgaaa
    13621 agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat ccagcgactt
    13681 atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag gcaaaaatgc
    13741 ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt gaagcttgtc
    13801 gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat gttccttgtg
    13861 aagtatgtca tggcaaacga tataactctg aaacattaga agtgcattac aaaggaaaaa
    13921 gcattgctga tattttggaa atgacagtag aagatgctgt agaattcttc aagcacattc
    13981 caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat gtgacgatgg
    14041 ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt gccagtgaat
    14101 tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg acaggacttc
    14161 atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat gctggtaaca
    14221 cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat atcattgatt
    14281 taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca ccagaagaaa
    14341 tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg gtataaagag
    14401 gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt gttttattaa
    14461 tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt atagacaaaa
    14521 acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag tttcctttga
    14581 ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa acaag
//
LOCUS       DAHEYX010000054        14583 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHEYX010000054 DAHEYX010000000
VERSION     DAHEYX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.055"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(297..1745)
                     /gene="cls"
                     /locus_tag="MB219_001139"
     CDS             complement(297..1745)
                     /gene="cls"
                     /locus_tag="MB219_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCC8426868.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1897..2790)
                     /locus_tag="MB219_001140"
     CDS             complement(1897..2790)
                     /locus_tag="MB219_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8426869.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2774..3460)
                     /gene="ftsE"
                     /locus_tag="MB219_001141"
     CDS             complement(2774..3460)
                     /gene="ftsE"
                     /locus_tag="MB219_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCC8426870.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3565..4666)
                     /gene="prfB"
                     /locus_tag="MB219_001142"
     CDS             complement(join(3565..4593,4595..4666))
                     /gene="prfB"
                     /locus_tag="MB219_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCC8426871.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4792..7326)
                     /gene="secA"
                     /locus_tag="MB219_001143"
     CDS             complement(4792..7326)
                     /gene="secA"
                     /locus_tag="MB219_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCC8426872.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            7652..8953
                     /locus_tag="MB219_001144"
     CDS             7652..8953
                     /locus_tag="MB219_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCC8426873.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(9049..9600)
                     /gene="raiA"
                     /locus_tag="MB219_001145"
     CDS             complement(9049..9600)
                     /gene="raiA"
                     /locus_tag="MB219_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCC8426874.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(9728..10447)
                     /locus_tag="MB219_001146"
     CDS             complement(9728..10447)
                     /locus_tag="MB219_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCC8426875.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(10444..11757)
                     /locus_tag="MB219_001147"
     CDS             complement(10444..11757)
                     /locus_tag="MB219_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCC8426876.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(11838..12158)
                     /locus_tag="MB219_001148"
     CDS             complement(11838..12158)
                     /locus_tag="MB219_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8426877.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(12266..12553)
                     /locus_tag="MB219_001149"
     CDS             complement(12266..12553)
                     /locus_tag="MB219_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426878.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            12680..13324
                     /locus_tag="MB219_001150"
     CDS             12680..13324
                     /locus_tag="MB219_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCC8426879.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(13383..14090)
                     /locus_tag="MB219_001151"
     CDS             complement(13383..14090)
                     /locus_tag="MB219_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8426880.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(14310..14480)
                     /locus_tag="MB219_001152"
     CDS             complement(14310..14480)
                     /locus_tag="MB219_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426881.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         4130 a         3131 c         2531 g         4791 t
ORIGIN      
        1 ttttcattac ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat
       61 tgtaaaaaaa actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct
      121 tttaatagca tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag
      181 ttaatttcgc tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg
      241 ttagaaaagg acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa
      301 aggagcggcg aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt
      361 tcatacattt cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt
      421 acacgtgaag ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat
      481 gaacgaacat cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc
      541 tttgcatgaa taaatccatt ctcatatgtt tcgatccttg ctccatactc gattagctct
      601 gcagcgaaag aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg
      661 atatgaactt ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc
      721 ggtatgtaat aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa
      781 taagtcattt tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct
      841 atatcaccag tagagtgaat ggacggaaaa aatttgggag aatagcctac ttcttttgta
      901 tgctgagaat tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa
      961 atacgcaaat gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt
     1021 ccaacattga acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga
     1081 ttgcgataat tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact
     1141 tctccgcctg cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat
     1201 gcatcaagaa ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga
     1261 acttcttcac ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct
     1321 gcctgcttga tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg
     1381 atttcatttt tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt
     1441 ttcacatcta cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg
     1501 tcttcttcaa tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc
     1561 tctttggaaa tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac
     1621 atcaaaacga gcacccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg
     1681 ataaaggaaa gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt
     1741 ttcacggctt cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa
     1801 agggaaaaaa gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa
     1861 actgtccaac tttctcattt tatttatatt gtggtattag aatttcaaga aacgtctcat
     1921 agaaatgatc gagccaagtg aaccaatgat cacaccaatg ccgaccatta aaagattgat
     1981 tttccaaacg atattttgtg gatgaatcaa tgaataatgg gaacgtaata atgatggatt
     2041 gatcaattgg aaaaaccatg cataaccaaa tgtgatgatc aaaatcggaa gaatcgcacc
     2101 gatcagaccg atccagccac cttcaaggaa aaacggccag cgaatatatc cattttttgc
     2161 accgactaaa cgcatgattt gaatttcacg ctgtctagac aaaatcgtga tccgaatcgt
     2221 gttcgaaatg agaaacatcg ctacgaacag cagcaatgcc gctgcaacta atccccatgt
     2281 ccgaactgca ccagcgattt ggaaaatccg atctgaggaa gctccaccgt aatcagaacg
     2341 agaaacattc ttcaaatctg ctgcttcttc tgctacttgt tttgtataag aaggatcagt
     2401 agcacttact acatacacat catataacgg gttattgtca ccttcaaaca atttccagga
     2461 atcacccatt tcgttttgga tttttttcaa ttcctgttct ttgcttgaaa attcaacttt
     2521 tttaacatga tctaagtcat tcaattcttt ttctagcttt ttcatctctt cgtcttttgt
     2581 accgatatct acgaatacag agacatcaac gtttccttct atatcttgag ctaatttagt
     2641 tgcgttcatg ataactgcca tgaatacacc gactaaagtc aatgtgatcg ttaccgcact
     2701 gactgaagca actgtcatcc agccgttacg tttcaaactc ttgatacttt ctaataaatg
     2761 gcggaaaaag gttctaatca tcgtatccat attccccttc cgcttgatct cgaataatgc
     2821 ggccgttttc gatagcaatt actcggtgcc gaatcgtatt tacgatcgta ctgttatgtg
     2881 tagccatcac gacagtagtt ccttgggcat tgatccgatc taataatttc atgatttccc
     2941 atgagttttc tggatccaga tttcctgtcg gttcatccgc gataagtact ttaggcgtat
     3001 tcactattgc ccgagcaata gagacacgtt gctgttcccc accggataat tcgctaggaa
     3061 agactcgaac tttgtgtttc aaaccaacaa ggtccagcac ttccattact cgttttttga
     3121 tatcccgagg ttttctgccg ataacttgca tagcgtacgc aacattttca tatactgttt
     3181 ttcttggtaa taatttatag tcttggaaca ccacgccgat ttctctgcgt agataaggaa
     3241 cttcgcggtt tttgattgct attagatcat gacctgcgac gttcaaagtt ccttttgaag
     3301 ctttttcttc tcgatacatc aatttgatga atgtagattt accggctcca gacggaccaa
     3361 ccacatagac aaactctcct tgttcgatga caattgagag attgcggata gcggtcgtac
     3421 catttgagta cttcttcatt acatccttca tttcaatcat ggcgttctct ccatatcttt
     3481 ttcagtcttt agctattata gcatggcaat gtttcaacat gcttgcaaaa tcttacaatt
     3541 ttatttcaat ttaacaatgg gttcttattg tagatgcatt tttaaatagc cgtcaataaa
     3601 taaatcaagg tcaccgtcca taactgcttg gacatttcct gtctctacgt ttgtccgatg
     3661 gtcttttacc atagaatatg gatggaaaac ataagaacgg atctgtgaac cccagccgat
     3721 ttctttttgt tctcctcgaa gtgcagctgc ttcttgttct ttcttttcca cttctaattg
     3781 ataaagctta gcttttaaca tccccatcgc ttgctcacgg tttttcaatt gtgaacgttg
     3841 tgcctgacta gctactactg ttcctgtagg caagtgagtg atccgtacag cagattctgt
     3901 tttattgatg tgctgaccac ctgcaccact tgctcggtac gtatcgattt tcaaatcatc
     3961 tggattgatt tctatatcga tcgtttcatc aagctctggc atcacatcta ccgaacaaaa
     4021 agaagtatga cgtcgtttcg ctgagtcaaa aggcgagata cgtacaaggc gatgaacacc
     4081 tttttctgat ttcagataac caaatgcgtt gtgtcctttg atcagtaggg tcacactctt
     4141 gattcctgct tcatcacctg cttgataatc tagtgtctct acttgatacc catgctgctc
     4201 tgcccagcgt gtatacatac gtaaaagcat actgccccaa tcttgggact ccgttccgcc
     4261 agcccctgga tgcaattcaa cgatggcgtt gtttcgatca taaggaccat ctaacaacat
     4321 agataattca tatgtcttca ttttttcatc tagcgcattg atacgtgttt ctgcttcttc
     4381 ttgcatggct gcatcaggtt cttcctgcaa catctccacc atcaattcga tttcttccag
     4441 ttcttcagca agagaatgga attgatcata agtttcttta tttgcattgt tttcactgat
     4501 cactttttga gcagcttcgc tatcatccca aaaaccaggt tcagccattt ttgcttctgc
     4561 ttccgcaatg tcttcttcca gctggtctaa gtcaaagaga cctcctgaag cttgtgacct
     4621 tttcctgcat ttctgtcagg agatttcgaa tttcggatat ttccatgttt tttccttctt
     4681 tcactatact tttttcatta tatctagtct aacagaatca gcaaataaca gaaagaaggt
     4741 gagacgaatt cttaaaaaaa gaatttgtct caccaatcct gctgtcagaa attagtttgc
     4801 attttttcca tgacagttct tatatttttt cccgcttcca caaggacaag gatcatttct
     4861 tccgaccttg ttcacatgga ttggctgttt tttcgcgcta gtattactat tttctttggc
     4921 atctccgtct tctgagtgag aagctgttcc ttgagaaacc tgttcacgct gtacgttttg
     4981 acggatctca gctttcataa agattcgagt gacttcatac tcgatggcgc ctaccatatc
     5041 ttcaaacatc ttatagcctt ctgtttgata ttcgactaat ggattgttct gtccataagc
     5101 acgtaagccg attgactgac gtaattgatc cattgcatca atatggtctg tccatttaga
     5161 gtccacaaca cgcaagatca cgactttttc aaattccaac agttgctctg gaccatttag
     5221 ttgagccgct ttggtattaa atacttcttg tgctcgttcg tttagatact ctttgatctc
     5281 ctgagctgtt tttccttcga tatctgcaag agtaatacta tcttcatgga caagagtact
     5341 gccggcaaaa tcgatgatac tttccaaatt ccaattttct ttctctaatt gtgtatgcgc
     5401 atctactaca cgactgattg tccgtttgac catgttcatt agtggcttgg ataagctttt
     5461 ttcttccatg attacttctt gtcgctgttt atagattact tcacgttgtt cacgcatcac
     5521 atcatcgtat tgtaagacgt ttttacgtgt gtcatagtta tttccttcta cacgtttttg
     5581 tgcagattct acttgtttcg tcagcatctt gctttggata acagcatctt cctcaccgat
     5641 cttcatccga tctaagaaag atttgattcg ttctgaaccg aatcgtttca tcaaatcatc
     5701 ttccaatgac aagtagaact gtgacatacc agggtctcct tgacgtcctg cacgtcctcg
     5761 aagctggtta tcgatacgac gagattcatg tcgttctgtc ccgataactg ctagaccacc
     5821 aagttctctt accccaagtc ctaacttgat atctgttccc cgaccagcca tgttggtagc
     5881 aatcgtcaca gcaccttttt gaccagcatt catgatgatc tctgcttctt taaagtgatt
     5941 tttcgcattc agcacttcat gaggaatacg ttcttgattc agcatatctg ataataactc
     6001 agatgtttca accgcaacag ttccgactaa cacaggttga cctttgcggt agcgatcttt
     6061 gatatcttgc acgacagcgt ggaacttgct ttgtaatgtt gggtaaagca aatctggacg
     6121 atcatcacgg ataaccggac gatttgtcgg aatctggtat acttggatgt tataaatttc
     6181 tctgaattct tcttcttctg ttttagctgt accagtcatt cccgcaagtt tcttgtacat
     6241 acggaaatag ttctggaaag tgatcgtcgc catggttttc gtttcgtctt cgatctctac
     6301 gccttcttta gcttcgattg cttggtgaag tccatctgaa taacggcgtc catccatgat
     6361 ccgaccagtg aactggtcaa caatcagtac ttttccgtct tggacaacat aatcgatatc
     6421 taaaagcatg atatagttgg ctctcaatgc ttgatccaag tgatgggtca atgcagtatt
     6481 ttcaatgtcg tataagttat ccaaaccgaa tgtttcttct gctttttcga tccctgcttc
     6541 ggttaaactg attgtttttg attgtacatc gattttataa tcttcttctt ctttcaaacg
     6601 tttcacgaag ttgtctgctc ttgtatataa agctgtggat ttttctgctt gccctgaaat
     6661 gatcagcggc gttctcgctt catcaatcaa gattgagtca acttcatcga caatggcata
     6721 attcagcgga cgttgtacca tttgatggcg gtaaacgacc atattgtctc tcaagtagtc
     6781 aaatcctaat tcattattcg tactataagt gatatcacaa ttataagcct cgcgtttttc
     6841 atccgaagat tttgagttga tattcaaccc cactgttaat cctaagaaat tataaagttc
     6901 gcccatttcc gtcgagtcac gggttgctaa atattcattg acggtaacta cgtgtacccc
     6961 ttcgccagtc agagcattta gatatactgg catcgttgcg gtcaaggttt taccttcacc
     7021 agtacgcatt tcagggatat ttccatcgtg aagcacgatt ccacccatca attgtacgtg
     7081 gtgtggatat aaccccaata cacgttttgc tgcttctcgg acgactgcaa aagcttccgg
     7141 aagtaattga tctaatgttt ctcctttttg gtatctgccc ttgaattcag cagtttttgc
     7201 ctgtaattgt tcatcagtta atgctgccat ttggtcagcg tatgcttcaa ctttttttgc
     7261 aatgctatct aaacgtttta gttctttctt atcgttttcg ataagtgaac gaatcaaatt
     7321 ggccatgcgt tgtgtaatct ccttttcctt ctgtgtatta tccagactat gtagacttta
     7381 cttttcattt tatcattagt tatagataat tgaaatatct taagcgtatt aaagtaagaa
     7441 cttcataatt tattcatatt tcatctaaaa gaggaggaaa ctcaaaaaaa cttgcggcag
     7501 ctgtcagtag ctgctgcaag ttttcgggct ctataatttc taggactgat agaaaatcat
     7561 tctatcggtc ccttttgagc atgaaaaaac aaaacataca gaagctccgt tagaattgaa
     7621 tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata tcaaactact
     7681 acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga gcattaagaa
     7741 tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct gtcctttgtg
     7801 taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat cgctcattca
     7861 acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac gctaccactg
     7921 tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa actgctttat
     7981 cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag caatgacgac
     8041 aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg cttctctgat
     8101 ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg atgaatttcg
     8161 ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg atactggaaa
     8221 attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt ttatgcaatt
     8281 tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg tttcttatgc
     8341 ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt ttcacattgt
     8401 caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa gctttgatcg
     8461 aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc ggcttctgat
     8521 caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt ttggttgggc
     8581 gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg aattacgaat
     8641 cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg cggacacttt
     8701 ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt ctataaaaaa
     8761 agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt attcaaatgc
     8821 caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt atggctttcg
     8881 aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta cctatgaatc
     8941 aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttatctc actctatcag
     9001 tcctatttga caaagagcca gttttcgttg ttatttaagt aaggctgatc agtctgtttc
     9061 aatcagtccg tattttccat ctttacggcg gtaaacgata cttgtaccat ttgtttctgc
     9121 atcttcaaaa ataaagaagt tatgtcccag catgttcatt tgtaagactg cttcttcact
     9181 atccattggt ttcaatgaaa ggcgtttcgt acgtacgata tctaattcag aatcattttc
     9241 gttttcgtcc tcttcattga ataaagcagc agcatctgca gtattcatgc ccgtttcacg
     9301 ggatttacga ttaatttttg ttttgaattt gcggatttgg cgttccaatt tatcaacaac
     9361 caaatcgata cttgcgtata aatctggaga agtttcttcg gcacgtaata caagataagg
     9421 taatgggatc gttacttcca cttttgctgt tttttctgta taaactttta agttcacatg
     9481 cgccgttgca tcgggtgcgt cactgaaata gcgttctagt ttgcctactt ttttctcaac
     9541 gtagtcgcga atcgcttcag ttacttcgat attttcgccg cgtacattat atctaaacat
     9601 aaaaattacc cctttcgtct accacaatca agaaggagaa ggaccactct ttccctcttc
     9661 ttaattctat tatatcgaac tctaggttat ttgcaaatta aatcgctatc aaatttccac
     9721 atctaattca tctcgccaaa gaaatggttc ggatgatttt cggctgtgcg acctgcaaac
     9781 aatctgctgc gtggaaaaga gtctgtcctg ttgtatagac atcatcaata atcaatattt
     9841 ttttattttg tatcttagaa aaggtgactg caagttcaaa aggttgaggc atctccaatc
     9901 tttcctttcg cgtgtttttt gcttgagcca gcagatgctt ctctcttttg agtagcatct
     9961 gaaaaggaat tcttgctgct ttcaacatct cgcttacttg attgaatcct ctttctgttc
    10021 gccttttttc agataaaggt attggacaat agatgtatgt cgggtattct ttttttatct
    10081 gcgtccattt atatgcaaag gtgcctgcta gtcggatatc tcccataaac ttgtattgat
    10141 tgagccaggc cttcatattt tcatcgtaca caaaaaaggc acggtgctga aaatcatagg
    10201 tcggatattt cttcttccat tcttgacagt cactgcagta aacgggaata gactttactt
    10261 tctttttcgt actctctaga ttgaaatgga ttggtttttg acagccagaa cagcctcttg
    10321 tttccaataa atgaaattgt gcgcgacaaa ctgaacaagt ctgaggtaat ctgtatttcg
    10381 aaacaccaat aatctcgctg aatgttaaat tttggaagat atctttactg cagcaactgc
    10441 acctcacagc atcttcttcc cttcttcatt catttctttg atttcctgac aagcttttcg
    10501 aatagctgca gttttttctt catagaaaaa gtatacttga ctgttcgtat aaggcccttt
    10561 acgatcggct cttcctgcga tttgtaccaa agaagattta gtaaaaaccg gatgatccgc
    10621 tcctaaaacg ataacggata ttctttcaaa tgtgactcca cgttcaagga tcatcgtggt
    10681 taacagtata tcatattttg cttctcgcat tttctggact ttttccgccc gctgattgtc
    10741 tcttgaagaa acatctgtga tctcatatct cggaagagcc tcttgaagtg tggctttcaa
    10801 acgatgcatc atcgtgatac ttggacaaaa taaaaggata tggttttctt taattaattg
    10861 atgaatgatc cgcaccaaag attttatctt cttcttgttc ttgcattttt gtcgccaatt
    10921 attcatgaaa accaagacgg gttcaggaag caaacgccga tgaaagcgta aagcaagttt
    10981 gtggatgtca aacgtctgct tgatttcctt tagtaacttt ttatcaggtg tggcacttaa
    11041 gtagatgaat cgtccgtctg gtttaatcgc tgtttgcaca gcatgagcta atcctgcatc
    11101 cccgttataa ggaaacgcat cgatctcatc gatcactatt aaatcaaagg accgataaaa
    11161 acggtatagc tgatgaatcg tacagataac aaaagaagta aaggtataag ccgttgctgt
    11221 attcccatgg agcaacgtga ttttttcttc tggaaaaaca taacgatagc gtaaaaacaa
    11281 ttcattacaa acatcgactc gcggtgtaca aatcgctact cttcctccag cttttaaagt
    11341 ttccactaaa ataggataaa gcatctctgt tttccctgca cctgttacag cccaaataag
    11401 atgatgtctt ttttctgaat ggcaaagtat tctctttgag atttccgttt gtttttcagt
    11461 aagctgtcct gtccaaaaaa agacgacttc tcgaggtttt gcttcaggct gatcaaacaa
    11521 atataaatct tgttgagtgt cgcatcgccc taaatataga caatgtgtac aaaaataacc
    11581 gtaatctgca agctgtccat caatgacgga cgtcctcatt ccacagcgat gacaaagaat
    11641 ccagttgccc aaacgttcca ctgctgcgat cttctcataa tttggtaaat ttacctcctc
    11701 acagtctatt gcttcaatat ctctcgtcag aagccttcta ccccaaaaat gattcatatt
    11761 cttcgcccta aaaaaatata cgaaaaaaca agcgatctat tttttggatc gcttgttcga
    11821 tagttatact gttctattta ttcacctatg agcgtgattg cttgatctaa cactttttcg
    11881 ccattcatca taccataatc agccatattg atgacatcta aaggaatgcc ttttggttcc
    11941 agacgtttct caaaatcagc tttcataaag cgaacttgtg ggcctaataa caacacatcc
    12001 acgtctttat ttgcaaggtt gttatctgcg tcagaagcgg acacagcgaa aatatccgag
    12061 tcgattcctt ttgcttcagc agctttttgc atttttgtta ctagtaaact tgtgctcata
    12121 ccagccgaac atacaagcat gattgttttt ttagccatac ttttctctcc tttttaaaac
    12181 aaactatgca aacgttttct tcgcgtctgt tactctatta tataaaaaga aagaaatttg
    12241 tcaacgtttt ctaaacgcgg tttatttaat caaatagcca actagatttc actaatttat
    12301 tgaacacgac atattctttg tccaaattga tgacaactac ttcatagcct gcatttatcc
    12361 agccataggc acctttttca gggtgtttat aatcatttgc ccaccaatct gtactttccc
    12421 ttgctgtttt gggaagtccg ttactgggaa acagcagttc gtcaaattgg gtaaaggtca
    12481 acgttacttg agagccgcca ttgttcttca aataatgcgt aattgcatcg tactttttca
    12541 ttcgttcctg catccccaga tccacctgcc tttgtcttta ttatcgcata ctacagcaat
    12601 tgcgcaaccg ttacagtgtt gaaacaacct agaactttat ttatactgaa taaagaaaat
    12661 gaaaaaaaga ggtgacaaga tgatcgagcg ctatcgtaca ataaaagaaa atggacaaag
    12721 tgagattgag atcaaaaaat cacgctttat ctgttctctt agacgaatag aatcagagga
    12781 ccaagcaaaa agtttcattc aagaaataaa aaaagaacat tggaaagcga accataactg
    12841 cagcgccttc atcattggag aaaaaaatga aatccagcga agcagcgatg atggagaacc
    12901 aagtggaacg gcgggcgtac cgatgttgga agtcctcaaa aaaaatcaac tgatcaatgt
    12961 agctgcagta gtcactcgat attttggagg aacgaaactc ggcacaggcg gattaatacg
    13021 agcatacact catgcggtct ctcaagcatt gtctgacatt ggaatcgtag aaggtacctt
    13081 gcaacaggag atctctgtga tgatcacgta tccgcaacta ggaaaattac agaattatct
    13141 tgaactccaa cattttttta taaaagaaat ccaatatact gatcaagtta gcgtcatctg
    13201 tgctgtagat gaaaaaagtg ctgaccactt taaagaatcc atcattgagc tgctgaacgg
    13261 acaagtccaa tttgaagagg gttctgtttc ttatcacgag caactgataa ctgatcatat
    13321 ataaaaacag gtgattctga ttctctttta tttctaataa ggaatcaggg atcacctgtt
    13381 ttttattttt ccaaataaaa ctcaaaacga tttcctacgt attgcgtacg cacatactca
    13441 aacggcgtac cgtcttcaaa ataagaaatc tggcgcaaac gaagaactgc atctcctcgt
    13501 tttacctcta aatagtcagc aattttctca gaagccaaca cagcagtaat cgtttgattc
    13561 gctggaccga tctttttccc gcctttttct tctagcgtgc gatacaacga ttcagtaatc
    13621 tctgctttgc tgtagccatc gatcagcttt tgtggaacac tggcaacttc aaaacaaata
    13681 gggacatcat ctgcgaaacg aatacgttcc attcgcaaga tcgtgtcatc tttactcaag
    13741 tttagttttt ccatttcact tgagcttggc gaagtcaaga aataagagat tgtccgacta
    13801 gacggtactc gattttgtga ttccataatt tctgtaaaac ttgttgcacc agacattttt
    13861 tcttgtactt tttctcgcgc aacatacgta cccgaaccga tctttctttc taagattcct
    13921 tcatctgcca agttttgaat tgcttgtctc aacgtcatac ggctgacatt gaatttcact
    13981 gctaattctc tttctgaagg aagacggtct ccgactttcc atataccctg ttcgatttca
    14041 ttttttaatt ggtcatgaat ctgtatatat acaggtaatt gattcgacat aacgtacacc
    14101 cgtcctttcg tttcttctat tataactggt atatacctga ttcacaactg aaaaagataa
    14161 aggtttctat ccatcaacag aggatttaga cacacatcgt tctccctact cctattgtca
    14221 gaaacaattt tttagatatg ttcaacctag aaaataacaa aagaccctaa aatcgatgaa
    14281 aatgccgact tatggatctt ttgttattat tagcggtttt tagccatcca tttttttgta
    14341 tgattcgttg ggcgttttga ttttacctgg accgcttttc cgcctttttt cggaatattc
    14401 acttcaatat tgtatttttc tgatttgacc aaatcatata acttttcagc tgcttcgtct
    14461 actttttctt tccatttcat gtggatccct ccaattcttg tagttaactt aactctatct
    14521 taattcagca gaatcttcaa ggaacaaggt agcaaagcaa aaaaaagagc tctcgaacta
    14581 acg
//
LOCUS       DAHEYX010000055        14454 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHEYX010000055 DAHEYX010000000
VERSION     DAHEYX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.056"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..209)
                     /locus_tag="MB219_001153"
     CDS             complement(<1..209)
                     /locus_tag="MB219_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426882.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISS"
     gene            complement(197..1549)
                     /locus_tag="MB219_001154"
                     /pseudo
     CDS             complement(197..1549)
                     /locus_tag="MB219_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(1546..2478)
                     /locus_tag="MB219_001155"
     CDS             complement(1546..2478)
                     /locus_tag="MB219_001155"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCC8426883.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2475..3251)
                     /locus_tag="MB219_001156"
     CDS             complement(2475..3251)
                     /locus_tag="MB219_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCC8426884.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3305..6490)
                     /gene="carB"
                     /locus_tag="MB219_001157"
     CDS             complement(3305..6490)
                     /gene="carB"
                     /locus_tag="MB219_001157"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCC8426885.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTYHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6495..7571)
                     /gene="carA"
                     /locus_tag="MB219_001158"
     CDS             complement(6495..7571)
                     /gene="carA"
                     /locus_tag="MB219_001158"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCC8426886.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7576..8856)
                     /locus_tag="MB219_001159"
     CDS             complement(7576..8856)
                     /locus_tag="MB219_001159"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCC8426887.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8875..9801)
                     /locus_tag="MB219_001160"
     CDS             complement(8875..9801)
                     /locus_tag="MB219_001160"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCC8426888.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9814..11097)
                     /locus_tag="MB219_001161"
     CDS             complement(9814..11097)
                     /locus_tag="MB219_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCC8426889.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11111..11650)
                     /gene="pyrR"
                     /locus_tag="MB219_001162"
     CDS             complement(11111..11650)
                     /gene="pyrR"
                     /locus_tag="MB219_001162"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCC8426890.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11885..12796)
                     /locus_tag="MB219_001163"
     CDS             complement(11885..12796)
                     /locus_tag="MB219_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCC8426891.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKPAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12793..13257)
                     /locus_tag="MB219_001164"
     CDS             complement(12793..13257)
                     /locus_tag="MB219_001164"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCC8426892.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLVLAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            complement(13262..13960)
                     /locus_tag="MB219_001165"
     CDS             complement(13262..13960)
                     /locus_tag="MB219_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCC8426893.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13947..14333)
                     /locus_tag="MB219_001166"
     CDS             complement(13947..14333)
                     /locus_tag="MB219_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426894.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINDEKRSVLSHEKKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
BASE COUNT         3912 a         3313 c         2583 g         4646 t
ORIGIN      
        1 gaagagatta ttctcttctt tctgctaacc agtcttagaa aaatgaattt taaaaaaatc
       61 aaaaactgat cacgatagaa gtttcttttc atacctgatg aaaagatgct tctcgcaatc
      121 agtttttgga tttttgctat tccaattgat gattctggag acacaaatcg gatttctcta
      181 tcttcatcaa gcgcgcttaa atctgccaat tttcaggatc ttttttccat tcttttagca
      241 gagttaattc ttgctcatcg atataacgat cttctaaagc tgcttcaatc aatacagaat
      301 aattggtcaa cgtcataaga gggatttcgg ctttttcgaa atttgctttt ccttttggca
      361 actcatacgt aaagatagcc gcaacaccaa ggatatccgc accttctctt tttgctgctt
      421 cagctgcttc caagacactt ccgcctgtag aaatcaaatc ctcgatcaca accatcttct
      481 gaccttcaaa tatacgtcct tcgatttggt tacctttccc atgatctttc gccttacttc
      541 gaatatagac cattggcaaa tccagtattt ccgctaccca tgcggcatga gggattcctg
      601 ctgttgcagt tccagcaatc acttgtactt cagggtaagc ttctttgatt ttgcttgcta
      661 atccttttgc gatctctttt cggacttttg gatagctcat cgtgattcgg ttatcacagt
      721 aaattgggct tttgattcca cttgcccatg taaaaggatt acttggattt aaaaatactg
      781 cttcaatttc taataagtct tttgcgatac tttgttcaac attcatttta ggttagctcc
      841 attctgtttg aatcgtttga tatgctttat aaggattagt tgactgagtg attggtcgac
      901 ctataacgat aaaagttgag ccgatctctt ttgcctgaga aggtgtgact actcttgtct
      961 gatctcctgc gactgttcct tctggtctta tacccggagt taagcaaaca aattcgtctc
     1021 ctgccatttc tttgatcgct tctacttccc aagcagaaga aaccacacca tctaatcctg
     1081 cttttttggc acagctagca taatggatca cacttttttc taaagaaact tcgattagtt
     1141 gatcggcatg catttcttct tcacttgtag aagtaagctg agtgatagct agaagtagcg
     1201 gacgttttcc accttctgga gtcccctctt ctaatcctcg cattgccgcc tccatcatac
     1261 gaataccgcc agcggcatga acacaggtga gatcaacacc aagctttgcc aatccccgca
     1321 ttgctttttc gaccgtattc ggaatatcgt gaagtttcag atctaaaaat acattatgac
     1381 cagcctcttt tagataacgt acgatttcag gtccttcttg gtagaacaat tccattccga
     1441 ttttcacaaa caatttttct tctttcggga aaaatcgaag aaagtcttct acctcttgcc
     1501 tagttgaaaa atctaatgca ataattggtc gttggtctag ttgattcatc gttcacgttc
     1561 ctctctcact tcttttatta atctttccag cgattcgatg cctagttcgc tcattcgcac
     1621 agggagctct ttaattagtt taggacagat atacggatcg gtgaaattcg cggtccctat
     1681 agctacagca cttgctccag ccataaacat ctcaagaaca tcatccactg tctgcacgcc
     1741 gcccatccca atgattggaa ggctggatat acttgctact tggtgaatca aacgaatagc
     1801 gacaggcttg attgctggtc cggagagtcc acctgtttga ttggctaaaa tcggttttct
     1861 tgtcttaagg tcaatccgca taccaagaag agtattgatc atcgtaaacc catctgcccc
     1921 tccagcttcg attgcctgag cgattggaac gatgtctgtt acatttgggg acaatttgac
     1981 atagacagga acagaagaaa ctttctttac tgcttgtgtt aattgaaaag caacatcagg
     2041 atctgttcca aaagcaattc cgccatgttt gacatttggg caagaaatat tcagctcgat
     2101 tgctttcaca tttggcgctt cgccaatttt gctacaaact tctacatagt cttcttcaca
     2161 agcaccagca acatttgcga tgatgggtaa ctcattgtac tcctctaatt ctggaagaag
     2221 tttatgcata acaacgtcta gaccaggatt ttgcaaacca attgcattca acatgccaga
     2281 tggtgtttcc gccactcttg gtgtgggatt gccaaagcgg gcatttggag tcgttgcctt
     2341 gaccatgatc gatccaagct gattcaaatc atagtatttt ccgtattctt tgccaaaacc
     2401 gaaacagccg cttgccggca tgataggatt ttttaattct agaccgggga gcttgactgc
     2461 taaaggattc tcattcatat aattacctct cctattttaa aaatcggtcc ttcgtcacaa
     2521 actttcacac tttttgttcc tgtttcatct tccggcgtat gacaaacgca agcatagcaa
     2581 gcccctatcc cacatgccat gcgggattct aatgataact ggacattttg atgcgaagaa
     2641 aacagctgtt ccactgtctt caataacccg tttgaaccac atgcaaaaac agctgtaggt
     2701 tccgtcttcg ttcctaacaa taaattaccg acatttccgt gcataccgta tgttccatcg
     2761 tcagtagcga ttcttgtttc tcctagacga ttaaattctt tttcataata aacgacttca
     2821 tgtgtggcaa aacctaaaaa atgcgttggc ttaattcctt tttcgactaa ctgttttgat
     2881 aactcatata gaggagggac accgattcct ccaccgatta taaatacttc gtctcctgta
     2941 tgaagattgg acaaatcaaa accattgcct aaaggaccca tgacatctaa atggtctccg
     3001 gcagaaagag tagagaagac tctagtcccc tcaccttcca cccgataaat gatacggcaa
     3061 gtatttgttt cgtggtcgat atgattcaag ctaatcggtc ggcgtaatag caagtctgca
     3121 cgcggcacga gaatatgcaa gaactgtcct ggttgctgca tttcttttac tagatcacct
     3181 ttcaatgtca tttcaaaaat ctttggtgcc agctgttttt gctgaacgat ttccatcatt
     3241 tcttgtttca tctgtcccct cctgcaacgg tttttcgtct ttttgtctta acatgtgctc
     3301 gccgttaaat agggttagtt gaaaaagcac gggattccat cactcgaacg atcgcatctg
     3361 ctgtgtctaa agaagtaaac aatggaatac catgttcaac tgcttctctt cggatgatga
     3421 aaccgtcttt agaagcattt ccccgatctt tatcaatcgt attgacaacc acttgtgttt
     3481 taccatctct tattaagtcg actacatttt tttctgctgc ttcctcagaa attttcgcta
     3541 ctgtttcaac gaccagacca tttttttcga aataagcagc tgtatgtttc gttcctatca
     3601 gactatagcc aatttctgta aaacgtttag ctaattctag tgcttcttct tttgtttcgt
     3661 ccgcaatcgt aaataatacg gctccatatt caggcaagtg taatccagaa gcttcgaatg
     3721 ccttgtacaa agctttgtct aattgtctgt cagatcccat tacttctcca gtcgatttca
     3781 tttcaggccc taagtaagta tcaactttct gcaatttcgt aaaagaaaag acaggtgctt
     3841 taacatgaac ttcttttgtt tctggataaa gtccgttttc ataaccaagt gtctttagtt
     3901 tctctcccaa aatagctttc gtcgcaattt gtgccattgg aattcctgtc accttactta
     3961 agaatggaac agtccgactt gctcgaggat tgacttcgat cacataaacc tcattttcat
     4021 ggatgacaaa ctggatattc atcatcccga cacaattcaa acctaatgct aagcggatcg
     4081 tgtaatcttc gatcgttttc ttgatcgatt cagataatgt ttgaggtgga tacacagcca
     4141 ttgaatcccc tgaatggaca ccagcacgct caatatgctc catgatccct gggatgagta
     4201 ctgtttcacc gtcacaaatc gcatccactt cgcattcacc accaagcaag taacggtcca
     4261 ctagtactgg atgttctgga gatgctttca cagcatgttc catgtaatct tctaaatctc
     4321 gctggttttc aacgatttcc atcgcacgtc cgcctaatac atagcttgga cgaacaagaa
     4381 ctggataacc aattctcgaa gcgataacca cggcttcttc tttacttgta gcagtatccc
     4441 ctaaaggttg aggaatatcc aactctttca gcgcttgctc gaataaatca cgattttccg
     4501 cacgatccaa gtcttcgatc gttgtcccta agatttttac accagcagca gtcaaaggtt
     4561 cggctaagtt gattgctgtc tgaccaccaa attgaacgat tactcctatc ggctgttcta
     4621 gctcgatcac attcatcaca tcttcgaagg tcaatggctc aaaatataat ttatccgaaa
     4681 tagaaaagtc agtcgaaact gtttcaggat tgctgttcat gatgattgct tcgtatcctg
     4741 cctgctggat tgctttgaca gaatgcacag tcgcatagtc aaattcaacc ccttgtccga
     4801 tacggatagg accagatccc agtactagaa ttgagggctt ttctgatcgt gaactttcat
     4861 tttcatattc ataggaacta taaaaataag gtgtttttga ttcgaattcc gctgcacaag
     4921 tgtcaaccat cttatataca ggaatcaaat cattttcttt tctcatctgg cgaacggctt
     4981 cttcgtttat ttcccacagc tccgccactt ttttgtctgt aaaaccgttt tgtttcgctg
     5041 ttttcaatat cttgtcatcc aacggctgct gtttcaactg ctgttcgatt tctacgatat
     5101 gcaacagttt atctaaaaag aataaatcga tttttgtcaa atccgctaat tcttctatgg
     5161 tgtaccctct tctaatagct tcagctacat aaaacaggcg atcatcttgt gctcgaacca
     5221 ctttttggat taggtcatcg tccgtgatgt ctgccaactc aggaagttcg atatggtatg
     5281 taccgatttc taaagaacgc acagctttca ataaagactc ttcgatattc cgaccgatag
     5341 ccatcacttc accagttgct ttcatctgcg tgcctaaaag ccgttctccg ttttcaaact
     5401 tatcaaacgg ccagcgagga attttagcta caacataatc taatgcaggt tcaaattctg
     5461 caaaagtcgt tcctgtaaca ggatttttca tttcgtctaa tgttaatcca agcgctattt
     5521 tagcagctag cttagcgatc ggatagccag tcgccttact tgctaacgca gatgaacgag
     5581 aaacacgcgg attgacttca atcacataat aattaaaact atgtggatca agagcgagtt
     5641 ggacgttaca gcctccctcg atcttaagtg ctcgaataat cgaaagagaa gcatcacgaa
     5701 gcatttgata ttcatggtca gacaatgttt gactaggggc aaatacgata gaatcacctg
     5761 tatggatacc caccgggtca aaattttcca tattgcagac aacgattgca ttatcagccg
     5821 aatctctcat cacttcatat tcaatttctt taaaacctgc aatacttcgt tcaatcaaac
     5881 attgggtagc gggggaaaga cttaatccat tttctgatac gatacgtaat tcttcttcat
     5941 tttcacacat acctccacca gttccgccta aagtaaaggc tggacgaacg atgacaggat
     6001 agccgatttt atttgcaaaa tctacagctt cttctactgt attgacgatt tcagactcgg
     6061 ggattggctg attcaattct ttcatcaact gtttgaatag atcacgatct tctgcttgat
     6121 cgatcgcaga aagctttgtt cctagaagtt ctatgttcaa ttcttctaaa atacctgatg
     6181 ctgccagctc cattgccata ttgaggcctg tctgtccgcc taaagttgga aggagagcat
     6241 ctggacgctc ttttcgaaga atccttgata caaattcaag tgtgatcggt tcgatatata
     6301 cttgatctgc cacttcttta tccgtcatga tcgtcgcagg attagaattt actaaaacca
     6361 cttcatatcc ttcttctttt aaggcaaggc aggcttgtgt tcctgcgtag tcaaattctg
     6421 cggcttgacc aatgatgatc ggaccagatc caatcaccat aattttcttg atatctgtac
     6481 gtttcggcat ctgtttactg ctcctttcct gcgtccatca tttccataaa ttcatcaaat
     6541 aaatgtagtg catcatgcgg accaggggca gcatctggat gaaactgcac actgaaagcg
     6601 ggataatctc tgtgtttgac tccttctact gttccgtcat ttacttctac atgagttacg
     6661 atgagttttt ctggatcaat cgtggcttca tcgactgcat agccatgatt ttgagaagta
     6721 aaatcaattc ttccggtcgc tacttctcgt accgggtgat ttaatcctcg atgtccgaat
     6781 ttcattttat aggtatctgc accgttggct aaagcaaata actggtgacc gagacaaata
     6841 ccaaaaatcg gtacttttcc ttggattttt ttgatcattt cgattgcttc tggcacatct
     6901 tttggatcac caggtccatt cgtcagcatg actccgtctg gtgatagcgc taaaatcgtc
     6961 tcggcatctg tgttataggg aagtactgtg aggttacagt ttcttttact caattctcgt
     7021 aaaatactat gtttcaagcc gaagtcaatg acaatgactt tccgacctgt tcctggactt
     7081 ggataaggtt tgttagttga gacttgctgc acttgattgg ttgctagaac ggttgctttc
     7141 agctgatcaa atgcatgttc gaaggaatct ccagcatcaa cgatactagc tttcatcgtt
     7201 ccgacttcac ggattttgcg agtaagggcg cgcgtatcga tacctgatat tccaggaatt
     7261 cctttttgtt tcaaaaatga atctagcgtc atttggtttc gccagtttga agctacacgt
     7321 gcatgttctt ttactactac tcctttgcat gttggagaaa tcgattcata atcatctcta
     7381 tttaccccat aattacccac cataggatag gtaaatgtaa tgatttgacc gttaaaactt
     7441 tgatcagtga tagtttcttg ataaccagtc atacttgttg taaaaacgat ttctccgaag
     7501 acactagcat ctgcaccaaa agcctttcct tcgaaaactg ttccatcttc taatataagc
     7561 aaacgttcca tacttttatt ctcccttctg ccacacaagt tttccgttga caaaagtcat
     7621 ttgtgtttcg ccttttactt tccagccgat aaaaggtgta ttctcccctt ttgataaaaa
     7681 gtcttcttta tcgatcgtgc aagtttgtgt aatatcaaaa actgctacat cagcgggggc
     7741 accaaccgtt agcgttcctg cattcaaacc aaaaatctca gccggtttaa ctgccaacca
     7801 attgatcact tgttccaacg tgaatcgacc agtttctaca aaatgagtat agatcagttg
     7861 aaacgctgtt tcgcttccta cgataccaaa tggtgatttc atgaaacttt ggcttttttc
     7921 ttctaatcca tggggagcat ggtctgtagc gatgcaatcg atcgtaccgt caagcaagcc
     7981 ttcgatcaaa gcttctctgt cctcccgtcc tcttagcggc gggttcattt tccaaaagcc
     8041 gaaatcttcg ggaatatctt cgtcgatcaa aataagatga tggggtgaaa cttctgctgt
     8101 tacgtggatt cctgcttttt ttgcgtctct gattacacgg acactttctt tagtagaaac
     8161 atggcaaaca tggtaatgaa caccagttgc ttcagctagc aacagatctc tagcaatttg
     8221 agaagactcg gttacgctta agatcccggg aagaccaagt tcttctgcct tcttgccagc
     8281 gtgcataacg ccgccaaata ataaagattc atcttctgta tgggctacta gggctttatt
     8341 gttttttgca gcttctttca tagccagata catcgtccca gctgtttgga cacctacacc
     8401 gtcatttgtg aaagcaaaag caccttcttc aatcagtgct tcttgatcaa ctaatttctc
     8461 acttcgcaga ttttctgtga ttggcgcata ctgcaatacc ttcacaactg catcttttct
     8521 aatgatgtca tatacttgtc tcagcttttc tgccgtatca ggtacaggat ttaaattcgg
     8581 catcgcgcat acagtagtga aaccgccacg agcagctgag cgagtcccag cttcgatcgt
     8641 ctccttgtat gtaaatcctg gctctctcaa atgaacatgg acgtccacta aaccaggtgt
     8701 gatcaattgt ccctttgcat caaacacttc atcaaattcg gcttctgaga atcctgtacc
     8761 gattgctttg attcgcccat cttctatcca aatttctgct ggtgtcgtca tattttttcg
     8821 tgtattgatc tgaccgtttt taatgagtgt tttcattttt tcatcctcct ataattacgc
     8881 ttttccattt agcactgctt ctaagattgc cattctgacg aatactccat tgctcatttg
     8941 ctgaacgatc cgcgactgta atccttccac taatgaatca gctaattcta catcgcggtt
     9001 aactggtgct gggtgcataa tgattgcatg tttttgcaac ttcttcgcac gctcaactgt
     9061 taacccatgt tcctgatggt agccttcttt tgagaaactt tcttttccat catggcgttc
     9121 gtgttgcacc cgaagcatca tcatgacatc aacttgatct agtagttcat ccaatggcat
     9181 ataatgtccg tacacttcga attcatcgtc gtaccattct ctaggacctg agaagaatac
     9241 ttgtgcgcct agacgtttta acatctgcat attggattta gctacgcgag agtgagtgat
     9301 atcacctacg atggcaactt tcagaccttc aaaatgacca aattcttcat aaatggtcat
     9361 caaatcaagc aagcattggg ttggatgctg tccgcttccg tctcctccat tgataatcga
     9421 acactggatt gttttacttt gtatcagttc gtcataatag ttctcatcac catgacggat
     9481 aaccgctacg tctacgccta atgctgacat cgtcagtact gtatcgtata gtgtttcccc
     9541 tttttggaca gagcttgtgc ttgcttcaaa ttcaatcact tctagcccta gttttttctc
     9601 cgctacatcg aagcttttat gtgtgcgagt actattttcg aaaaagaggt ttgtagcaaa
     9661 gtactggcgc ttttgcggag tccaattagc accttgcttg aattcctgcc ctctgcggat
     9721 caagcccata acttcttgat cagtaagtgc ctcaacggtc aataaatgct ttaagctgat
     9781 acgttctgat ttgatgatca taatgtttcc tccttagttt tcttcgcttc gtgaagtttc
     9841 tggtaagata agatttaaaa tgatgccaag tacagttgct aatgccattg ccgacaatgt
     9901 aaatgtgcct acttcaaaga cgagtccgcc aattcctact actaagataa ctgaagcaat
     9961 caacagattt ttctttttat caaaatcgat tttgttctcg attaaaatct tcattccact
    10021 tgccgcaatt actccaaata agataaaact gattcctgag atgactggtc cagggatact
    10081 taaaatcaaa gcgcttaatt ttcctacgaa tcctaaaatg actgcaaata ctgcagctcc
    10141 accgatcaca aacacgctat ggacacgtgt gatagccaga acaccaatgt tttcaccgta
    10201 acttgttaca ggaggacctc cgacaaaggc tgccacgatt tgtgctaacc catcacctgt
    10261 caacgtctta tgaagacccg gttcttcaaa gaaattacgt ttagttagtt tattcaatac
    10321 cataagatgg ccgatatgct ctgtcatcgt cacaaaagcg atgggtgcca ttgttgtgat
    10381 tgctccaaga tatagctttg gttcatattg tacaaaggga atctcaaagt taggaacagc
    10441 aaaccaggca gcgtctacga ttggcttagt atccacgatt ccaaacagca aggctacgat
    10501 gtatccgcta atgataccta gtaaaatcgg aatcaatccg agaaaacctc ttagccacat
    10561 gttgaagaaa attgttaatc ctaatgtgat cagcgctaca gcaatatact tgaaatcata
    10621 cgtgccctga ttatacatag catttgttgc tgcatttgcc gcaagtccca acccgataac
    10681 catgatgact ggaccgacga cgattggagg caagatttta tccagccatg ctgaaccgat
    10741 ctttttgatg atcagcgcaa caatcaaata gactaatcca gttgtgactg ctccttgtgc
    10801 aatcgctgga taaccatctg ttttcatcaa aagctgcatc gctgcaatga aagcgaagct
    10861 gcttcctaag tacgcaggta tctttccttt agtagtaaat aagtacacca atgtacctag
    10921 tcctgaactg actagcgcaa tacctggatc gatacctact aagatgggga ctaacacggt
    10981 tgctccgaac atcgtgaaca agtgttgtaa acttaatcct acccaatgtc ctggggttgg
    11041 gcgatcgtgg atatctaaca ccacgtcatc attatgaaat ccttttttct ccgacatgtt
    11101 tctctctcct ctaatcttcc tctttcaaaa tcatgatacg atcttgtcca tcaagctctt
    11161 gcatctctac taaaatctct tccgcccgag atgttggaat gttcttccca acataatctg
    11221 cacggattgg caattctcga tgcccacgat cgaccaggac tgctaatgaa atttttcttg
    11281 gtctgccaaa atccattact gcgtccattg ctgctcgaat cgttcgtcct gtatacaaaa
    11341 catcatcaat caaaataact tcttttcctt ctaaggaaac tgggatgtca gaagaatgaa
    11401 gttccggttc ttctggattt tcttttttgt catctcggta taaagtgata tccagttctc
    11461 ctacaggaat atcgatatct tctagctgct tcaggcgttc ggcaattctt gatgcaatgt
    11521 agattccacg tgttttgata cctactaata caatatcttg gatactatga tttctctcga
    11581 taatctcgta agtaattcga gtcaatgctc gcttcatcgt tacctgatct actacttctt
    11641 ttgcttgcat attcattccc cctacttttt tgcataaaaa aactcctgcc ctaagaggac
    11701 aggaggaatg tttagactga gcgtatactg atacactgat agctatacta tgtcaatgag
    11761 caatctactt atggcgcacc atacgtatcc ttgctcccac ccttcttagc ctcacgggac
    11821 taaacttaaa ggaattctat taagttttat gctttcgttg ttaattacta taccgttatc
    11881 taaattaaaa tgcaatgttt tttcttaatt tttctaaagt tttttcaaaa acttctggaa
    11941 gaggtgcttc aaagatcatc ttttcgtttg tagtcgggtg cgtaaaacct aacatttgcg
    12001 catggagaaa ctgaccattg cctttcaaag ttttcttcgg tccgtatact ggatctccag
    12061 ctaacggata accgatatac ttcatatgca cacggatctg gtgtgttctt ccggtttcta
    12121 gttttaattc aaccagagta aatccttcga aacgttccaa tacagtaaaa tgtgtgacag
    12181 ccggcttccc atcttctctt atcgcctgca ttttccggtt ttccttcgaa cggccgattg
    12241 gcgcattgat ggttcctttt tcgtgaggaa tctctccatg gactaaagca acatatttgc
    12301 gcaaagaggt cttatctttt aattgctgag ctaatgattc gtgtgcctgg tcatttttgg
    12361 caaccattag cagtccggaa gtatctttgt cgattcggtg aacaatacct ggacgaataa
    12421 cgccgttgat cgtagaaaga tccttgatat ggtacatcag cgcattgacc attgtgccat
    12481 gcgtatgacc tgctgaagga tgaacgacca ttccttgagg tttgtttatc accgctacat
    12541 cttcatcttc gtaaacgatc gtcaacggga tattttctgg gaccacatct aatgtttctg
    12601 gttcggggat caagacagta atctcgtctc cttgttttac tttgtaattt gctttgactg
    12661 gctgtccatt gacagaaacc gctccatttt tcagccacag ctgaatttgc gaacgactgt
    12721 aatcggtgaa taaattagtc aatactttat cgatcctacc agattcatgg tgaatcgtcg
    12781 ttttaagttc tgtcattttt ttctttgatc cttttcatct aaaataatat aaattaatat
    12841 acaaatcact ccacatacaa gtgtcacatc tgctacgtta aaaatcggaa agctcataaa
    12901 atctgtctgg aacatatcta ctacatagcc aagacgaata cgatcgataa aattgccaat
    12961 cgcaccagct agaaccaaac tcaatccaat catgaaccag cgattacctt tttcacgatt
    13021 tttaatcatc aatgtgagaa tcacaacgac tgcaatgagt gtcactatag taaagaacca
    13081 catcttccct tctagcatac tccatgcggc tccagtgttt cgaatatgat tcagtgacaa
    13141 aatacctggt ataagatttt tcgtctctcc aagcgctaga ttatcagtga tccaccattt
    13201 gaccaactga tccactccta ctagcaaagc gctgaatatc caataaataa ttaacaaaaa
    13261 atcaatcctt ttctatttgt ttataaatat cacgaggtcg tacgatcccg agcagattac
    13321 catcgggatt cccatcttta gtgataagta cggcttccaa agtcccttgc tcacgaaaca
    13381 tcgcttcaac ctcgtaaata tgtgttttag ctggaacaaa atgataattt gtgtactttc
    13441 cgttttggat gatcaaatcc aacgctcgtt tatttgttaa atcaaccgga cctttttggc
    13501 tttcttttgc tagccaaaac ccgatattac gtaaagtaat caatgctaca aattttcctt
    13561 tattgtataa cggaaattgt gaataccttt tttctgctac tatctttaac agttcaagta
    13621 acggaagatc ttgttcaaaa cctgttacac gttttgcaaa tcgaggcagc acggtttcag
    13681 gctgaatcag ttcttgttcg atcagctgga ttcgcttgac tgtccaatca tttggctctg
    13741 caatgacaaa atcgactgcg atccgatcat ggacgatcgc attccttaac tgagcaagct
    13801 gtaaaagatc atcttcgtat tttctgatca ttaattgttt tctttgagct aggcgtctga
    13861 caagttcggt aaatcccata tttcgtgcat ttcccatctc gtctctcatc catttttcga
    13921 ttcgattgaa actgattaaa aaaagatcag agtttgccat aattttcctc cttgatcaaa
    13981 tatgcccggt tttttatata gaaaaagaac gcaatcaaca caataactgc aaaaaaactg
    14041 aaaaataaat tttgtaggaa aacggccccc aacccagttg ctagaaataa aaacggatag
    14101 atttgaagaa tcaagtaatt cattgtgatc acataagcag gctttgcttc tttcactgta
    14161 taaacggggc tttttagcca ataaaaggtg acctgcatcg taaagggcac ttccagatca
    14221 agcgttatct ttttttcatg actgagaaca ctgcgttttt catcgttgat tcttagctga
    14281 atcgagctac ccataccgta aaaaccggtt tttcttataa atgtcgtttt catcataccc
    14341 tcctattatg ctttagtata gctcaaaata caataaagaa aaagaatgat cctagtgtaa
    14401 acaaaattaa ttccctatcg tctaaaaaaa gaagctgtag tcaaaataca gctc
//
LOCUS       DAHEYX010000056        13845 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHEYX010000056 DAHEYX010000000
VERSION     DAHEYX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.057"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(132..497)
                     /locus_tag="MB219_001167"
     CDS             complement(132..497)
                     /locus_tag="MB219_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCC8426895.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(644..1417)
                     /locus_tag="MB219_001168"
     CDS             complement(644..1417)
                     /locus_tag="MB219_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCC8426896.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(1591..2451)
                     /locus_tag="MB219_001169"
     CDS             complement(1591..2451)
                     /locus_tag="MB219_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCC8426897.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(2451..3869)
                     /locus_tag="MB219_001170"
     CDS             complement(2451..3869)
                     /locus_tag="MB219_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8426898.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(3926..4375)
                     /locus_tag="MB219_001171"
     CDS             complement(3926..4375)
                     /locus_tag="MB219_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8426899.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(4820..5083)
                     /locus_tag="MB219_001172"
     CDS             complement(4820..5083)
                     /locus_tag="MB219_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCC8426900.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(5182..6477)
                     /gene="rho"
                     /locus_tag="MB219_001173"
     CDS             complement(5182..6477)
                     /gene="rho"
                     /locus_tag="MB219_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCC8426901.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(6474..7745)
                     /locus_tag="MB219_001174"
     CDS             complement(6474..7745)
                     /locus_tag="MB219_001174"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCC8426902.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(7910..8779)
                     /locus_tag="MB219_001175"
     CDS             complement(7910..8779)
                     /locus_tag="MB219_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCC8426903.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(9071..10681)
                     /locus_tag="MB219_001176"
     CDS             complement(9071..10681)
                     /locus_tag="MB219_001176"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCC8426904.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(11874..13214)
                     /gene="glnA"
                     /locus_tag="MB219_001177"
     CDS             complement(11874..13214)
                     /gene="glnA"
                     /locus_tag="MB219_001177"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCC8426905.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(13242..13634)
                     /locus_tag="MB219_001178"
     CDS             complement(13242..13634)
                     /locus_tag="MB219_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCC8426906.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         3921 a         3018 c         2349 g         4557 t
ORIGIN      
        1 aaaaaaaccg tacagatctg aaaataatct gagcggtttt ttgagtaaaa atagtttatg
       61 attattctta attaaacatc tatttatttt taaagctttc atttatcgat aagaatagtt
      121 taactgttga tttattccat tttccgttca tctggtacga tgccttgact tagcaaccac
      181 ttgtggaaga accattctgc cacaccgatc aatagagcag gaatcaatac tttcatccaa
      241 atatcgactg tatcattcat aaaatataga tagagccaca agataactgc agctgaacca
      301 gcatctgcga tcgtcgctgc gatgttccct actttacgta agatcaacag atcccctagc
      361 acatagatca tgactacaat aattacccca acaatcgttg aatccataaa gctaacaccg
      421 tataaacctg tcagcacgac ccacagaatc gcccagatca tgattgcctt cactaccaac
      481 gccttcacat gtttcatttt ctgcactcct ttctcctgga taaaaaagta agattatcta
      541 acaagataac cttttcatta ttataccatc ggctgagaga agcgagcaaa gataaggtcc
      601 tatctaaaaa tgaggaagcc acttagaaca ttcacgtaat aatttatccg tacagacatt
      661 cgatatggtg ttcctttact ttcgcatact gttcttttgt cagttcttta cttgtcacca
      721 gataatcgat ttcatgaaaa gcaatggctt gatgcatgaa tttatgtgca attttttgct
      781 gtgtaactaa aacggcaagc cggtctgatt gttttctaaa agcttttttg accattgcct
      841 ctccttcatg agtttctgtt actccttggc tgaaatctag cccgcgacaa ctcattatcg
      901 ctaaatccgc ataatatgac aatgcatcgt tataggcttt tgaaccgttg attgttgctc
      961 gtttcggaac aattgcccct cccaatacag atacttttgc cgttgtatat tcactcaaca
     1021 tagcagctgt gacgatgcca ttcgtaatga tctgcaaacc atcaaatctt gctaggtttc
     1081 tgactaagtg taaagaggtt gtactggaat ccaagaataa gaccatatca tctcgaatca
     1141 aagaagaagc tgtatctgca atcttacgtt tttctgtttc ttcttctaat ttggtgatct
     1201 tcatcaggta aggcacgtca atttttcgat tgtcaataag catggcgcca ccgcgaactc
     1261 tacggatcaa tccacttttt tccagaaaaa caaggtcacg tttaatcgtg gcttcgctat
     1321 aatttactaa ctcgatcaaa tcggtgactc ttgcaaattg gttgtttttt agataattca
     1381 caatctcctg ctgtctattc tccacttcat tcaacataaa atcaccccat ttactttata
     1441 tagtaacagg aaaaaagagg ttgtgacaga agtgattggc ttcgagaaat aagaagaaat
     1501 ttttcgaaaa aactacttct gaacatcgtg tgtggttaag agggtatgac aagactattg
     1561 tcataccctc ttgaccacac atttttattt ttagtataaa ctagccttgc ctactgtgtt
     1621 caaaatttcc attttatgtt tgactacttc ttgtaccttt ttattagctg ctggataaat
     1681 tgcattcggc tcgtacatcg atggattttc actcaatatt tctctcaaag cattgaaaaa
     1741 gacgctttta agatctgagc tcaaattcac tttggcaact ccgtatttta ctgcttcgct
     1801 gacttctgcg tccggattac ctgaaccgcc atgtaaaaca aatggaagat cgatttgttg
     1861 gttcaattct ttcagtaagt cgatatttaa ttttggagtt ctgtttttcg gatacaagcc
     1921 atgcgctgtt ccaattgcta cagcaagtgt atcgattcct gtttctttca caaaaatctt
     1981 cgcttgatca ggatctgtat aaatgattgt ttcagcacct ccttcagcag aaccattatt
     2041 cccaatcgtt ccaagctcag cctccactga gacatttacc gcatgagcta acgcaaccac
     2101 ttttttcgta atctgtacat tgtcgtcgaa tgatttcaaa gacgcatcga tcatcacgga
     2161 agaatagccg tttcgaatcg cccgtaagac atcttctatc gtaccgccat gatccaagtg
     2221 gataacgatc ggcagtttac ttgtataagc ataggctctt actgccgcaa caaactcatc
     2281 acttaaatag gcgatttcat caggatgaat ttctaagatt actggtgctt ctaatctctc
     2341 tgcttcttcg atgattgctt tcatcatatc atagctgcaa atattgaagg ctggtactgc
     2401 aaattgattc tctttagcca ctttcaataa ctctttcatt gtatataaca ttacgcttcc
     2461 acctcatttg ttagattttc atttttcgtc cacttgtctt gttttttctc tttcactgtt
     2521 tttttcaata gtccaagcat aaaggctgta gtgactgtac ctgatgcaat tgccaagaca
     2581 tacataggta aattgttcat aacatttgga attagcatca cccaaatccc tccatgaggt
     2641 gccattgaag taactcctac tgccatggaa atccctgcag cgactgctga acctgtcatc
     2701 aaactaggta tcacgcggat cgggtcagat accgcaaacg gtatagcacc ttctgtaata
     2761 tacgaagcgc ctaaaatcca gcatgattga cctgaagtcc gttcttcttt ggtaaattta
     2821 tgtttgaata ataaagtggc taaagcaata cctaggggag gtgtcattcc tgcagccata
     2881 caagcagcaa tcggtgcatt tactccagct gacatcaaac cgatcgagaa agtcgaaatc
     2941 gatttattga tcggcccacc catatcagct gccatcatga tcccgataag taatccgaac
     3001 aatagcccgg atgtatttcc caagctattc agccaatttg tcaaagcatc caacacccat
     3061 gtgatcggtt gttcaatcac aaataccatc aaaagcccaa taattaagat cgataaaaag
     3121 gggacgacaa tcagctgata aatagcagaa aattcttttt ttacgctaat tttatttgcc
     3181 agaaaatcac agatataccc tgctagcacg ccaccaatca ttccgccaag aaaacctgaa
     3241 ccacctgtag aggccagtaa tccggcaaca attcctgggg caaaacctgc ttttccagca
     3301 atcgatgtag caatccccgc gcctaacgca ggaaccatca tggcaaacgc tgtatctcct
     3361 ccgatccttg aaaatgcagc tgctaacaca ttatagtctg ctgagttggg atcagccgct
     3421 gtaatcccaa aggcaaaact cagtgcgatg atgattccgc ctgcgatcac aaatggaagc
     3481 atgtaattca caccattcat gaaatgatta tagattgtcg gctttttctc atttgcagct
     3541 ggttcttttt cattagacac aacgaatacg ccaatatctc tcaatgcttc gtctattact
     3601 acttgcggtt ctttgattgc tcggcttgta gagacacgct ttactttttt cccggcaaat
     3661 cggtccaatt cgatttcttt atctactgca agaatgattg catctgcctc tttgatttct
     3721 tgttcagata actgattttc tgtttttaca ccttgtgttt cgaattttgc ttgatggttt
     3781 agatttcttg ctgttacttc taatttttct gccgacatat aagtatgagc aattccagtc
     3841 gggcaagcag ttacaccaac gattttcatg attattttcc tcctttttct tcactatcga
     3901 tactcggaca aagactctac tattttcatt cttgactaat ctgtctgatg atatctgctg
     3961 gcgtagtggc ttcttttatt ccctttctaa aatctgcatt catcaatcgt ctggagagtg
     4021 tgctgagaat ctctagatgc atatctgctg atttttcagg aactacgatg gcaaaaatat
     4081 attcaattgg catttcatct atcccgctat agtccgttaa tttggctgta tgaaaaaaac
     4141 acagtgctgg ttttgtcaca tatttgcttt ttgcatgggg aatcccaaat ccatcttcta
     4201 ttcctgtgga gatctcctcc tctcgtttga atagtgcctt aacgactttt tgaacatttc
     4261 ttaccttatt tgtttttttc aacacatccc ctattcgctc aaataatgat ccttttgaat
     4321 ctatcgatgg atcgaaaaca atcagttctt ctgcgatcaa ttgatcaaac ctcataaaaa
     4381 acctctcccc cacaaaaaat gttaacgttt tcttggaact gaggtcagta tagcatcgct
     4441 agctgtaccg ttcaatatta tgaatgaaca gaggaaaaag acccatcatc cgcgtgtata
     4501 agtcattaaa atgaacgatc tagctcacaa acaatcgcaa gtctataaca aaacaaaaag
     4561 ccttccttga aaggaaagct tagcttctaa ttttaggtaa atggtttttt acttcgaaat
     4621 aacccctctt catttcgtta atataaggtg tttttatcaa gtggataatt aaaactatat
     4681 actagttttt cattgttttg tgcattatga tcctacgact ttttcagcat caaacgaagt
     4741 tttaaattgg caaaaaacat aaaaaaacag actatcccta tcagatagtc tgctaaacgt
     4801 tgagttatca gcgtttttat tattccgcag cgtttgcgtc tttgagaccg tattttttgt
     4861 tgaaacggtc cacacgtccg tctgcttgtg tgaatttttg acgtccagtg tagaatggat
     4921 gtgagtcaga agttacttcg acacggatga ctgggtatgt attaccgtct tcccattcaa
     4981 ctgtttcttg tgaagttttt gttgaacctg acaagaattt gaaaccagtt gttgaatcca
     5041 tgaatactac tgggtggtaa tctggatgga tgttttcttt catgattttt tcgctccttt
     5101 gccctgctcc atttgctgaa ccagagttga tttgtcgatt tacaacatta acatcttaac
     5161 atgtttcagt caaggttgca attatctttt cagatttctg gtcggatttt ttttaccaaa
     5221 ggagacatct tgaaattctt tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg
     5281 gatcagttgt tccgtatatt ctaatgaatc tccagtcata ttttttcgaa gtttccaaat
     5341 ttcttctaat tcttcacttg gcatcaataa ttcttctttt cgggtacctg attttttgat
     5401 atcgatggct gggaagatcc gacgttcaga taattcacgt gaaagttgta attctagatt
     5461 tccagttcct ttgaattctt catagatcac gtcatccata cggcttcctg tatcgactaa
     5521 agcggtcgct aaaatcgtca aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa
     5581 tttcttaggt ttatacaatg cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg
     5641 tggaacgact aagttatagg cccgagcaag tctagtaata ctatccatca agataacgac
     5701 atctcgttta tcttcgacta aacgcattgc gcgttccaat actaattcag aaactcgagt
     5761 atggttttgc ggctgcagat caaatgtaga agataccacg tcgcctttca cgcttcgttc
     5821 tagatccgtt acttcttccg gtcgttcatc aatcagtaga acgattaatt ctacttccgg
     5881 atagttttcg gtgataccat ttgcgatttc ttttaataca gaagttttcc ctgcttttgg
     5941 cggtgcgacg atcaatccac gctgaccaaa accgattgga gaaaagatat cgatcattcg
     6001 ggtagatagt ctttgcggtg ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt
     6061 taaggctgga aaatgcggac gttcttttgc ttcttccgga tcttttccat taacgctttc
     6121 cacgtgcatc aaaccataat aacgttccga ttctttaggt ggacgggctt ttcctgccac
     6181 tttgtcccca tttctcaaac tgaatcgtct gatttgtgag gcagaaatat agatatcttc
     6241 tgcacttggt ccataattga ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc
     6301 caatattcct tccatataat aaaatccttg tttctctgct tgtgcacgaa tcacagctag
     6361 agataattcc tttttattca tcttactgta ataaggaatt ttaaattctt tggcataact
     6421 ataaacttct tttaacgttt tgttctctag ttctgccatt gtcaaataat cgctcattca
     6481 gctcccactt cctctatcat ttcaattgat gcccctaagg ctgttagttt ctcgataata
     6541 tgatcataac cgcgtaagat ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat
     6601 agtccagcaa tcactaaaca agcacctgcc cgaagatcag aagccacgac ttctgtgccg
     6661 tgaagtttat ttggaccatt cacgatgatc atatttcctt ccacggtaat atcagcgccc
     6721 attcgtgcta gttccggcac atggttcaca cgtttcgcat agatcgtatc gatgatttct
     6781 gaagtgcctg tagtcatcaa tagtaacgca gtaagtggct gctgcagatc tgtggcaaat
     6841 cctggataag gataggtcat gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg
     6901 atttcatctt cgctgatttt catatggaca ccaatttcct gcaattttgc tataaagctt
     6961 tccaaatgtt caaagatcac atttttcact ttgataccat ttcctactgc agctgctaat
     7021 gctagatagg ttcctgcttc gatgcggtca ggaatcatga aatgccgaca gccatgtaat
     7081 gtttcgactc cttcgatacg gatcacatct gtacccgctc ctcgaacttt tgcccccata
     7141 ttattcagta aagttgccac atctatgatt tccggttctc gtgcagcgtt ttcaattact
     7201 gtctgtcctt ttgcttttac agcagctaac atcacattga tcgttgctcc gactgaaacc
     7261 acgtccatga aaatccgatt gccacgcaat cctttatttt ctgtccgtaa atacatggca
     7321 ccatgttcat ttgtcacttc agcacctaga gcttcaaatc ctttgacgtg taggtcaatc
     7381 ggtctagggc ctaaataaca gccacctggc aggcctacta ccgcttcgcc aaatttacca
     7441 agcagtgttc ccataaaata ataagaagca cgcaaactat tgatttttcc gtgcggcatc
     7501 ggaatcgata cgacttctcg aggatcgatt tctagtatgt tgttggaaaa atggacagtt
     7561 gcgcccatga tctctaaaat ttcaattaat gaatgaacat cttgtatgtc cggcacacct
     7621 tccaagatca ctggggaatc agctaagatt gcagctggaa tcaatgcgac tgcactattt
     7681 tttgcaccgc tgatcgttac ttcaccagct aaaggtcggt ttcctttgat tacgattttt
     7741 ttcatctgtt tccaccttgt ttattaatct tttttcatgg atatgcttgt attttatcat
     7801 agataaaaaa aagaaacgag catggtctgc cgttttaagt aaaaaagaat ggagagcgac
     7861 ttcaaaagtc gttctccatt cctatatatc gtatctgtta tgtggatgct tacgctttgt
     7921 tagctgaacc gaaccagtcg atatgagatt tagcatcttc aacgattgct gctttacctg
     7981 gagctaataa tttacgaggg tcgaatcctt taccttcttt atctttacca gcttcgatat
     8041 atttacgagt agctgcagca aatgataatt ggaattcagt gttaacattt acttttgaga
     8101 tacccattga gatagctttt tcgatttgat cttgaggaat acctgatccg ccatgtaata
     8161 ctaaaggcat gtctggacct acagcatcag caattgcttg taagtggtca aaagctaatc
     8221 ctttccagtt ttctggatat tgaccatgga tgttaccgat accgcaagct aagtagtcga
     8281 tacctgtagc aaccatttgt ttgcattctt caatatcagc taattcgccg ttaccgatga
     8341 ttccgtcttc ttctccgccg attgaaccaa cttcacattc aactgagata ccttttgcat
     8401 gtgctttttc aacaacgtct ttagctaatt tcaagttttc ttcaaatggt agatgtgaac
     8461 catcgaacat tactgaagta tagcctaatt cgatacattc caaagcagct tcgtaatcac
     8521 catggtctaa gtggatagca actggaacag tgatgttcat tgcttcaact aaatcagtga
     8581 ttaaatcttt acagattttg taaccaccca tgtatttagc tgcacccatt gatgtttgga
     8641 taagtactgg tgcttgtttt gcttcagccg cttcaaggat tgcttgagtc cattccaagt
     8701 tgtttgtgtt gaatccgccg acagcgtaac cgcctttacg agcagctttt aaaaattcag
     8761 ctcctgatac tactggcata ataaaattcc tcctaagttt tgtaaaacta aatttgttaa
     8821 gacaattctt aaccaacgac cctattttac caaacatttc ggtagttttc tactaaaatc
     8881 aatgaaaatg aaaattttca agaagttttt tgcaaaaaat aaaatatcca atagcgcttt
     8941 aatcgaaaaa agtcttttgc tttctatctt ttttcctaca aataataaca aatcagaaaa
     9001 agagaaaagt gacaagaaaa ttgcttatcc ccttctcttg ctacttttct ctttctatca
     9061 agttcaaaaa ttattttgtt tcgtgatctg ctaatgatgc tccgacaaat gctttgatta
     9121 atttttgcgg gcgatttgga cgagaaatca attctgggtg gaattggcat cccacaaaga
     9181 attttttctc tggcagctct acgatttcta ctaaacggtt atctggtgag acacctgaaa
     9241 agactagccc ttgttcttca aacaattgac gatatttgtt attgaactca tagcggtgac
     9301 ggtgacgttc ttgcacaaca tcggctccat catatgcttc agccgtcttc gttccttttt
     9361 tgattttaca tgggtataat ccaaggcgta aagttccacc caaattctcg atgttttcct
     9421 gatcagccat taaatcaata atattgtttg ctgtatctgg attcgtttca gctgatgacg
     9481 catcttctaa tccaacgacg ttacgtgcaa attcgacaca agccatctgc ataccaagac
     9541 aaatacctaa aaacggaacg tcattttcgc gagcataacg aatcgcttcg attttccctt
     9601 ctactccgcg atcaccaaat ccaccaggaa ccaagattcc atctgcgtct ttcaaacgtt
     9661 cttctacatt ttctctcgtt aattcttgtg ctttgatcca atcgatctcg atgtctgaat
     9721 tataggcgaa accagaatgt ttcaatgctt caactactga gatatatgca tctggtaact
     9781 cgacatattt accgacaagc gcaatccgta cttttttctt caagttcaag acacgttctt
     9841 ctaattcttt ccattctgtc atgtctgctt ctggtgtatc tagttttaaa tggtcgcaaa
     9901 caatttgatc cattccttgt gcttgtaaaa ctaatgggat ggagtaaagt gtatctacat
     9961 ctggcgactc gataactgct tctggttcga catcacaaaa ttgtgccaat ttgtttttca
    10021 cgctttgaga aacaggttgc tctgtacgta ccaccaaaat attcggttgg atacctaaac
    10081 cacgtagctc ttttacactg tgctgcgttg gtttagtttt catttctcca gcagctttca
    10141 agtaaggaat caatgttgta tggatataca taacattatc tgtaccgaca tctgctttca
    10201 tttgacgaag tgcttcaagg aatggcaaag attctatatc ccctactgtt ccccctactt
    10261 ctgtaatgat cacatctgaa tcagtcattt tagctgctcg catgattttt tctttgatct
    10321 catttgtaat atgaggaata acttgaacag ttgctcccaa gtattcgccc ttacgttctt
    10381 tacgcaaaac ttcagaatag atttttcctg tcgtcacgtt ggagtatttg ttcaaattga
    10441 tgtcaataaa acgttcgtag tggcccagat ccaaatctgt ttcagcacca tcatctgtta
    10501 cgaatacttc tccgtgctga tatgggctca tcgtccccgg atccacgttg atgtatggat
    10561 caaatttttg gatagttact tttaagccgc ggtttttcaa taagcgtcct aaagaagctg
    10621 caacaatccc ttttccgatt gatgaaacga caccgccggt cacaaaaata tattttgtca
    10681 taagttcaaa aactcctttt taatttatgt gcaggaaaag tctcagacaa taaacaaagc
    10741 tccctattct tcaataagaa tagggagccg aaatttctgt aaacttctga ccctctaaaa
    10801 gggtgcccaa ggtgtataat acaagctgac taagggaaag tcaagtactc aaatgaatac
    10861 tatccgctta caattttttc tttttcgata tcctttgaga aattatttct taagtccttt
    10921 atgagtgcaa aaaaaattta acccacataa taagcttgat ataaacatag ctaacgttaa
    10981 acctaagcaa atcagaaaat cgttactgaa aaattttaag tgaaataata gataaaaaaa
    11041 taaaacaaac attatgatag ataatatccc acctaacaaa gaactacttt ttcctacttg
    11101 cagcaacagt ttatatagct tatcgtagaa acaatctttt tctttcaccc ttatttacct
    11161 cctttacgta aaaaacgtag tccacagttc tcggattttt ggcatatctt ttctattaaa
    11221 taatatcaaa aacaaaacaa ttccactaat tttttcaaag atgacctttt tttcaacaaa
    11281 agtgccttgt agggattcat tgaaggtaac gaatcacata agtaaaaaat aaggagatgc
    11341 gacaaaagtc gtgcctcctt atttctaaat aaacggtgtt ctcctgccaa ctcctcggtc
    11401 acaagtcaca atatccatca aacatgagaa gctgtttttg aaattatttc ttgtgattcg
    11461 gagttaaatg gtaggctcac aacctctgga taaacggtat tcaaaagcca gtttctcggt
    11521 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttaatact
    11581 cgaaactaaa cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc
    11641 ggtaataagt tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt
    11701 acttgttgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc
    11761 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta
    11821 atactcaggt caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa
    11881 agttctagat attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg
    11941 agacgttttg cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca
    12001 ttatccttac gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt
    12061 gcagcttctc tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt
    12121 gtgatctgat tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat
    12181 gggtttgcag atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct
    12241 ggtacacgta ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca
    12301 taacctggaa cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca
    12361 cgagcatgtt tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca
    12421 ccgtctttat catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata
    12481 ccagaaccat tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg
    12541 cgtgcaatcg ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca
    12601 tctgcatatt taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc
    12661 acttcaaagc ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa
    12721 tctgttggtg caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg
    12781 tcttcgtcta atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa
    12841 ccaaggtcgc gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat
    12901 ggtgtcccat ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt
    12961 tcccacggga agatcatcca agtggagaga tctggataca aatacatatc gctttcttca
    13021 attcgaacaa atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg
    13081 tcgatctggc tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc
    13141 aatcgtaaaa atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc
    13201 tgtttcttta ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt
    13261 tgcaatcctt gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca
    13321 tcacgaaaaa tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt
    13381 tttttcgcct gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa
    13441 agatagtctt taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt
    13501 ccttcgcttc gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca
    13561 gacaaatcag tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat
    13621 tccttttctc ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta
    13681 aaaaaaatat tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata
    13741 aaataacaaa aaaataagaa cacaaaaaag gtatctagtt gattttcgta ccagatacct
    13801 tttatttttt attatactgt actatccttt attagtgttt ttatt
//
LOCUS       DAHEYX010000057        13609 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHEYX010000057 DAHEYX010000000
VERSION     DAHEYX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.058"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..314
                     /locus_tag="MB219_001179"
     CDS             <1..314
                     /locus_tag="MB219_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426907.1"
                     /translation="FYNWNSWEKYTKNMKEIQKEFPQIVKSSPVDISGTMVGTGNSPI
                     SSYTADYYTTNQKGKMVELITQTRNTPTTATTTIWRGVTSIGENQLFYLEDLDSYVRA
                     E"
     gene            736..1473
                     /locus_tag="MB219_001180"
     CDS             736..1473
                     /locus_tag="MB219_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426908.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(1490..1906)
                     /locus_tag="MB219_001181"
     CDS             complement(1490..1906)
                     /locus_tag="MB219_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426909.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            2298..3545
                     /locus_tag="MB219_001182"
     CDS             2298..3545
                     /locus_tag="MB219_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCC8426910.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3813..6203
                     /locus_tag="MB219_001183"
     CDS             3813..6203
                     /locus_tag="MB219_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HCC8426911.1"
                     /translation="MKYEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            6252..8087
                     /locus_tag="MB219_001184"
     CDS             6252..8087
                     /locus_tag="MB219_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCC8426912.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            8156..9019
                     /locus_tag="MB219_001185"
     CDS             8156..9019
                     /locus_tag="MB219_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCC8426913.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            9009..10118
                     /locus_tag="MB219_001186"
     CDS             9009..10118
                     /locus_tag="MB219_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCC8426914.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            10457..11257
                     /locus_tag="MB219_001187"
     CDS             10457..11257
                     /locus_tag="MB219_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426915.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            11345..12151
                     /locus_tag="MB219_001188"
     CDS             11345..12151
                     /locus_tag="MB219_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426916.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(12173..13600)
                     /locus_tag="MB219_001189"
     CDS             complement(12173..13600)
                     /locus_tag="MB219_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HCC8426917.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         5031 a         2029 c         2657 g         3892 t
ORIGIN      
        1 tattttacaa ctggaattca tgggaaaaat atacgaagaa tatgaaggaa atacaaaaag
       61 aatttcctca gatagtaaag tctagtcctg ttgatatttc tggcacgatg gtaggcactg
      121 gtaatagtcc aatatctagc tatactgctg attactatac aacaaatcaa aaaggtaaaa
      181 tggttgaatt gattacacag acaagaaata ctccaacaac agcaacaaca acaatttggc
      241 gtggagtaac aagcatagga gaaaatcaat tgttttattt ggaggattta gatagctatg
      301 taagagcaga gtaaaataaa aaaagagcta acggtaccaa cgttagctct ttgtgctgac
      361 gatgataaca aaacctaccg aaaaaggatt gtttcatcac aaacacaaac agaaaagaat
      421 tgataaaaaa cccgaacctc agaattttta ttaatcaatt catttagatt ataataaatt
      481 gagctgaaca ttacaatacg tatatgttgt aaaacgtaaa aaaagtcaaa agtaaatact
      541 ttcttaaaaa gtcaaataaa actattaaag acaatatata tggtttattt gacataagaa
      601 aatggtctag atgataattg taatatagaa ttgtagactt aaataaagca taaaatacct
      661 tttatgataa ggtatagctc ttaattttaa gcagttttag atataagatt gaaagtaaag
      721 tgggggagaa taataatggc agatcataaa ttagaaaagt tattagaaga aacgtggaat
      781 ccaaaagaat ttagtgaatt ttttatggag aattttgaaa ctgacttagc agtgattgtt
      841 aaagatgctc ttagagagca agggtatcct gaaactgcaa actatataaa cattaatttt
      901 actttgtata ctgaaaataa aggaacttgg gatttttggg ctactttagc aaataaggag
      961 ttaagtgata aatcagacac aggaattcgt aacttttttg aatcaaacag agatgattat
     1021 atgtatgcta acaatcaaaa taaattgaac tttagagtag agtttgatga aacacctgaa
     1081 gaatttatag aaagacaacc gcctaaagaa aatgtagcaa aggtattgga agatcgttgg
     1141 aattcagatg aaatagtttc aacaattagc gaactaggcg gacaatatga accgctagta
     1201 gaagcggtta gagaagagct aagactgaat aagtttcctg atgttcaaaa tattgatgtt
     1261 tctcaaattg aaataaacgt caagatcacg aataaacttg attatggtag ttgggcagat
     1321 atagcattag aaaaatacat ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat
     1381 atcatgtatc tacagcatcc acagtattta aactttggtg ttgaaatagc tacacctctt
     1441 gaagaatgga aaatggagca agggcttgac tagcctttgc tccatttttt tattttacaa
     1501 atgaatacag ttcgtcgttt ttaccaagaa tgattgtttg cccaactttg agattgtcag
     1561 gtgatccttc agcggtttgt aaggagaacg ttccattgaa cttatcggat accgttatag
     1621 gtagctttaa ttctgatcct tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat
     1681 aaacagtctt tattaaataa tttttcgtag tgatctcagc agatttgtct ttttttgtag
     1741 tagtaccagt ttctttagaa ttagaggtat tttctacgac tattccatct ttggcatttg
     1801 agcttgtgtt tatttgattg aatattttca gcgacaagat aacgctaaga atagtcaata
     1861 tagaaacaag tgtaattaat actgataatt tccttttttt gttcattttt tcttaacatc
     1921 cttttttgtt ttgattagtt agatattcct tatctaacaa gcctttttag gctattaaat
     1981 gaagataaac cccaaaaaat attgcaatta tgacagtgca atggtactat tattatacaa
     2041 taaattttgc gaaaggggta ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa
     2101 tacaacttgt ataccgcaag ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt
     2161 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
     2221 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
     2281 aaaaacaaaa agaaggaatg aatatgaaga gaaattcgct tggtatggtt ttattggcat
     2341 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
     2401 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
     2461 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
     2521 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
     2581 gacgaacttt caacatcagt aaagatacat taacagttaa ggaagcagat ggcgaagtag
     2641 ttagtcaaac tgttcaaaaa gataaacctg atcctattga aaatacggag caagagaaca
     2701 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
     2761 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
     2821 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
     2881 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
     2941 atcaatcaca aacaggttcg agtgatgaaa ctgacagttc tacttcacca agtaaacccg
     3001 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
     3061 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
     3121 taatgacttt tgttaaatca atgccactat ctcctgacaa taatccaaat aatggttttt
     3181 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
     3241 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
     3301 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
     3361 aacctgctca acataaacca atgttttggg gcgatgctat ttggaatcga catgtagtag
     3421 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
     3481 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
     3541 gttaataaaa gatatcgttt atatactaag tagttatata gtctttattt gtccttgctt
     3601 aactacttag aaaacttgtt gctcatggtg ggtagaacta aaaggggtga tgctatgaat
     3661 actagagcgt tgaaacagct tgctttcaac gaaaaaaatg attaataggt gtggtgattt
     3721 taacttagga atgggcgcga aggtcattac cacaccgaca tatcgtggct cattttcttc
     3781 gcctaactgg tataaagaag gagtaacgag agatgaagta tgaaaaaacg ataattttaa
     3841 aatcaaaaat aatttattgt tttgcgactt tagtaatgat gttatctacg cttataatgc
     3901 ctttttcagc aataagagct gaagcagatg tcacaaaacc gacaagtgca aaatttatag
     3961 aaactggcgt aaaaacagac ggttatatta gggttaatat gccgaatcat ccaaatgaat
     4021 ggatgatttc aagtcaattt aaagattctc atggaaatat tggatattgt atggattcag
     4081 aattgccatc cccaacagga agtggagccg gaagcttgaa gtacaaggga gctggcagtg
     4141 atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg
     4201 caggaaatga tacagaagca tggtatgcaa cacaattagt ttcatgggtt ttagccggaa
     4261 actttaaagt ttcacagatt gtatggtcac atccaaacca taccgccgca gaaacagctc
     4321 gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa gaatggaaaa gatacgccca
     4381 acactgagtt ttctatcaca gcttctaaga cagctgacga aggaaaatat cacactttca
     4441 catataaaac agcatcaaat aagactggta atgcgaagct cacttttaca tcagcaaaac
     4501 cagctggtat gaagatttat gatgcagatg gtaaagaaat tactaacaac actgtgaaat
     4561 taaattcaag ttttactatt aaagtacctg tgactacacc aagtggtact ttgtcattta
     4621 aaggtactgc taatgttagc acaaccaatc cttttacttt tgatggacga ggtgtgtatc
     4681 aagatgccgt agttatgatc actacttcag aaacaaagga ctctaaatct ttgagtgcta
     4741 aatggacaag agctgttggt caggtaacag ttaagaaagt tgatcaagat ggaaatgctt
     4801 taaaaggagc ggagtttact ctaactgaca ataacaatgc taaatctgtt aagacaaccg
     4861 gagatgatgg gttagctacg tttgatttgc aacaaggctt aacatacagc ttagaagaaa
     4921 caaaagttcc tgaaggttat caaggttcat ttaaaaagga tggtattact gttaataccg
     4981 atggacaaga atttagttac actgctacga acacaattaa aaaaggtaaa gtaaaagtta
     5041 aaaaggttga ccaagatgga aaaccattag ctaatgctga atttaccttg acggatgatt
     5101 catcccaaac acaagcagaa aaaacagatg aaaacggact tgcaagcttc gatattgtag
     5161 caaacaagac ctacagcctt gaagagaccg gagtaccaac tggttataca ggcgtattca
     5221 agcaagaagg aattaccgtt aaagatgatg gacaaacttt tgaatataca gctacaaaca
     5281 ctttgaaaaa aggtttagta aaagttcata aagtcgatca agatggaaaa cctttggcaa
     5341 atgcagagtt cactttaacc gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa
     5401 atggacttgc aagcttcaat attgtagcaa acaagacgta tagcctagaa gaaaccggag
     5461 taccagtagg ttacacaggt gcatttaagc aagagggtat caccgttaaa gacgatgggc
     5521 aaacttttga atatacagct acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag
     5581 tcgatcaaga tggaaaacct ttagcagatg cagaattcac cttaacggat gaaaaaggtg
     5641 taaaagaagt taaaaagacg gatgcaactg gtctggctac gttcaacatt attgcgaaca
     5701 aaacttataa cttagaagaa acaaaagtac caacaggtta cacaggcgca ttcaaacaag
     5761 aaggaattac cgtcaaagat gatggacaag tctttgaata tactgctaaa aacactttga
     5821 aaaaaggtgt gatcaaagtt cataaagtgg atgatcaagg gaaagcatta gcaaacgcag
     5881 aattcacttt gacagacgac aaaggtgtaa aagttgtaca aaaaacggac agcaatggct
     5941 tggcaacatt taatattgtt gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta
     6001 atggttatac agggaaatgg tctaaagaag gcatcaccgt tgctgacgat ggacaaacct
     6061 ttgaatatac agtaattaat caccttgtac ctgaaacagt aagaacaggc ggaatcggta
     6121 catcagcctt ccttcttgta tttctaattg caggattgag tatggtgcta ctgtattatc
     6181 gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag
     6241 aaggctgacg tatgaaatca aaaaaaattt tttcaaattt aatgctattt ggtactttgc
     6301 taggagtggg attgggcgca agctcaatct cttctagcct tggactaggg gggcaagtag
     6361 catatgcaca acaaaactat aattatcaag gaactggaac gattcatatc cataaagtaa
     6421 caaatactaa tggggatggg aaccctgtct cagatattga taacaaaggg aaagaaatag
     6481 ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt ctcttatgtg aaacttacta
     6541 aagatgaagc taaatccttg ggatggacgg atagtgatac tacaaaaggt caacatattg
     6601 atgatgctaa agcacgatta ttactaaaca aaaaatacag tgaagtaagt agaatgaaga
     6661 ctgattcaca agggaatata gacataccta tacaagtaaa tacagacgat ttaacagata
     6721 acttgtattt gattgttgaa acaggaacag tcaaaggtgt agttgaagaa gcgtatccta
     6781 tgattgttaa cgtacctgaa ctaaacggat cagatgatca atactttaat tatgatgtac
     6841 atgtctatcc taaaaactat ttccaattaa actcagcaga gcttgataaa aaagacgaag
     6901 atggaaaggc tcttgccgga actgtttttg aattgcgaaa agatgatgga tctgatcagg
     6961 ttgttggaac ttataaaaca gacagcacag gtaaattaac tgtagatggt ttggaattag
     7021 gtgactatta tttcctagaa accgatttgg gaacagatgc taataaaaca acctatttac
     7081 tagatggtaa aaaattcaaa tttagcgtta aacgtgggga gactgctaaa gtagttcttg
     7141 atgttaccaa ctacaaacga cctgtaatta ctaaggtaat tgataaagaa accgttgata
     7201 ctggcgaaga tgttcaatat acattgaaac catcaattcc tggtaatatt ggtaattacg
     7261 aaaaatatgt tgtcaaagat gttctaggtg cagagttaac ttatgattca tcagaagtga
     7321 tcgaaggaat gactttagat actgattaca gtatcgaacg tgacggacaa acgacaaact
     7381 atgtctttac tgaatcaggt atggataagc ttcagaaaca atttgatgca ggggatcgct
     7441 tctttacaat tttgatgaac gctcatacta atagtaaagc taaaatggat acaccactta
     7501 agaatgatgt ttcaacgatc tacaacaatg gatatgaacc cgaagacgaa acaccagttg
     7561 atcacgctta tacaatcatt ggtgatcacc aattcattaa aacagatgga gatacaagca
     7621 aagcattgag tggagcaaaa tttgtagtga agaacaaaat tgatccgaat aaagttactt
     7681 acctaaaaca agatccaaca acgaaatctg ttacttggat tgcagataaa tctaaagcta
     7741 ctgaattcgt ttccggatca gatggtaagt tccaaattga aggcttgcaa tatacctatg
     7801 atgtagattt tgataaacaa ggtaatccaa caatcacaga taaaaaaatc aatgattatg
     7861 ctcttgaaga agttgttgct ccttcaggat atacaccaac tagaaatgat gttccatttg
     7921 tggtatcgaa tgatgatgct tctagcgaag taacagacat caagaacctt aaaacacctg
     7981 aaacagttcg tacaggtgga attggaacag tagtatttct agtggttgca ggtgtggcag
     8041 gaacagggct atatgttgtc tacaaacgag aacaacacaa agcttaatga aataaaattc
     8101 cctagagtgt ttttggctct agggtttctt tttaaaaagt gtgtgaaaag taggtatgag
     8161 catgaaaaag aagaatatca agttgatcat cttaaaaatt gggatgttgc tatgtttagg
     8221 agtaggtcta tatcctgtaa ttactcaggc ttatacctac taccaaactc aacaaacact
     8281 aaactcctat aaggaaaaat atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa
     8341 gagatattca gaagttaata ggaaacgaaa agacacagaa caaacctcag tcacgattcc
     8401 tgaagataat aacaatattc cgtcaaatgg aaatcttcag aaggataaat taggtgaatt
     8461 agtgggtgaa attcttatac caaagattaa tgtggatttg ccaatttatg atggtgtaaa
     8521 cgatttccaa ctatcagcag gagcaggaat attaaccgga actgattatc caactggtgg
     8581 gattggaaac cattctgtta tatcagctca ttcaggcatt cctaataaaa aatattttac
     8641 agatttagag ttgctcaaac ttggtgataa attttttatt gatatttcag ggaaaaaact
     8701 tgcttatcaa gtagatcgga taaaaacaat tctaccaacc gaatttgagg atttgcttcc
     8761 tgtggcagga aaagattatg taacacttat gacatgtaca cctgttggag ataattctca
     8821 taggttactc gttagagggg ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa
     8881 gaagaaagaa gatttaaagg taaaggcgca gatgaactgg tatcctatcg tagttgtagg
     8941 ggttttatta ataatagctg tgatagtcat tattaaaaaa atagtagcaa agagacgagg
     9001 aatgaaaaat gaagaataaa atactacttt ttttgactct tatttgcact ttggttttaa
     9061 caatttatcc tgcaaatcag ctctatcatc aattcaaaac aaaagagaat gtggcggaaa
     9121 tccaagagaa aattgagaaa aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg
     9181 aacaaataaa atcgaacagc ggtaaccaag gattagacaa tactcagaaa acagataaag
     9241 gagtagacaa tactcaggaa acagacggtg gatcatttga tattcgtgaa gctgaaggaa
     9301 aggtcaatcc atacacagga gatgtttttt caaaaaatca aacgaaagat tttgaaccat
     9361 ctacctattc aggagcggtt ggttatgtct atattccaaa aataaatgtg ctagagcttt
     9421 tgtttctagg cgcttcacag gagcatttat atagaggtgt ggcacaaatg agtgctacta
     9481 gtctaccttt atcaggtaaa ggtcatcaaa cagtaatagc aggtcatcgt ggcggttata
     9541 cgtcaccaca atttctttat gttgatcaat tagtagccgg agaccacatt tatatagatt
     9601 atcttggtga aaaagcagtt tatacggttt tccgaacaga cgttattaga aattacggtc
     9661 agaattcaac actgactgat attcctgatt tagatatgtt aacactttat acttgtcatc
     9721 catatcctca gaactacgat cgtttattag ttagaagtct aagagatgaa aaagcaaaat
     9781 acatcacgcc acaagtaaca gttggcaata ccgcatacgc tgatgccatt gatagcgagt
     9841 ccgtttcaga taagacagaa actgttacat cttctaatga tttatttgaa gatgttgatc
     9901 gtgccaacaa ggaattagat ggaaataatg atcttaatat tccggaacaa ggtagcgtag
     9961 agatgcaaga cagaacgttt gctgaaaagg caagttcaat atttacagga attaccaata
    10021 aacatcagaa aatgctttta gtcatttgga ctgtaatagg agcattagca agtctaaagg
    10081 ttcttattct tttaatcaaa gagtttcgaa aagcataatg aagattataa aaattgatag
    10141 agtacagtaa aaagggattg atactattat tgatctcttt tttattttga atagagctag
    10201 attcactttt aaataaaatc aaaactgatc ttaattctaa agaaaaagtc aagaaaaaaa
    10261 tacaataaat ttttttatca gaatatttat cagaatatta ttagaaaaga taaactcaaa
    10321 agcttaattt aatagggtta ttggttgtat ttagaaataa tgtatggaaa agctataaag
    10381 caatagataa gatgtagaaa atcttatttg tgaatgaaaa acaagtcatc gtaaaatatt
    10441 aataggaggt gtagtaatgg tgggagtatc aaaaacagct ttagactcat tagctaaact
    10501 atttagtagc aggtttgatg gtgcagaatt tgttgaatat ctaagccaaa acgaaagtgg
    10561 aaaaactctg atcgaaacaa tgaaaaaaga ctatttgaat gaaaatgact ttccgacatg
    10621 gacgaatatt gaagatgcta catgggatgt agtatgtacg atatattctc cttgggtaac
    10681 gattgctgat tttgttttag ataaaattgg aggggataca taccctgatg ccaaatctct
    10741 tgctacaagt gactttaaaa cgttctatgg ttatctaata ggtctaatag gcaatggaat
    10801 agatttgaaa gttacgccaa aacaaacacc tgaagaaatt gaaaaagaaa aaggagaggt
    10861 tcaaatggct tatgcaaatg acagagaaaa agtaaaatta ttggaaggta aattatctga
    10921 agaagaactt ggaaaaatgg tgcaagaaat caatgaagac agcaactatt ttcctgaatt
    10981 gaaggtatat caaaatgacg aagatttctt taatgaaatg tttagtaata gtccatggga
    11041 agcagtaagt aaaacaaatt taggagaatg gaactttcaa gatgatctag ttgcatttag
    11101 caatgatggg tatttacgat catattcaga aagtgagtat agagctgaat taaaaacgta
    11161 tgaaacggat attctgatga gctatttaaa tctattagag agtggtcagg gagagttaag
    11221 agataaagtt gaagaactat taaaagaaga gctataggga ttatttcaac ataaacacaa
    11281 gcagaaaact accaaacaaa acgtaccatg caaaaagtcg gttaaaccgg ctttttgctc
    11341 tattatgaga ggagatcaaa ccatgagtgc aacaaccgat atggctttag aagcagtaat
    11401 aaaagataat acaggaaatg tcgaaaatgt agaagacttt agcgatattg caatcaatct
    11461 ttcagcatgg atttcagctg taggagaaga aaatatcgaa tttgagatca agaatatgga
    11521 tgacttaaaa gcaaaatttc cttttattgc atcgaaacta agtcaagttg ccgaggataa
    11581 atcgtggaat gaattagtta gtttttccga aaaaatttat gatattacgc aagaagatga
    11641 gtgtgtttca tgggaaatga ataatcaata caagaaaatt gacgctttcg tcaaatcacg
    11701 tatagaagga acagcttttg agtttgaagg tatagatacc gcagataatt ggttaaataa
    11761 agaatccttt attaaaagta tagcggatga tcttgaaaaa caatggtcta aggaagacta
    11821 tgcagagtac ctaaaaacag aaagagatac agaagtgaaa gaaaaactca ctgaggtatg
    11881 tttggatgaa catattgatc ccaatatcgt aaaacaagaa gactatgaag tggttattga
    11941 taaagttcct actgattttg aggaagttgt aaagtatata gttgaagaag cattatcgca
    12001 atactcttca gatgaggaag tggacgaaga aactgttcgt accagagttt ggcaactggt
    12061 ttttgatgac aaagattata aatatttgag tgatcaaaac tcggttaatt ggagtgtacg
    12121 attattaatt gatcaaaatg atattgaata aaatagagct gaaaagctct attcagattg
    12181 tagacaaaaa ggagttggta attaagccac tcctttttgt tagtctttgc ataattttga
    12241 aataaaaagc cataggcttt tttgttttcc atagccatgt ggctagtttt tttaaattca
    12301 tggcagcaaa cgtaagcatc gtgtgcgtgg cgactttttc aagacctcga tatttcgtcc
    12361 aacgcatacc atgcttttct tttgcatctg caaatattcg ttcaatggtt tgttttctct
    12421 ttttgtaaat ttctctattc aacgccgtat ggcgttggtg ttcaagttca tccataagat
    12481 cagcccaaat atgtcgagtc actatttttt gatgttttgt actcaaagta caattttcaa
    12541 gtaatggaca actcacacag aattttggat ttgatttata ggttcgataa ccatcacgag
    12601 tgatagttga aaataacaac ggttgattat tgggacataa atagcagtca aaataggcat
    12661 cataaacaaa gtctttttta cgaaacattc ctttcactcc atgaggtgca gtatatggaa
    12721 gaatcgggta gagtttcaag tgtgacagaa aatgaacaaa tttaggcgtc ttatatcctg
    12781 catcagcaac cacacgcttt atatttggaa aagctctttt cgactgttta accagatcga
    12841 ttgcgacttg gctgtcatgc acatttcctg gtgtgattaa tgtagataag ataaaaccgt
    12901 tatcttcaca ggaagtatgt gcagaataag caaattgttt ttctcgttca cctttaacat
    12961 agtaaccact ttctggatct gaatgactta ttttttgaag actcaactca ctttctgtgt
    13021 cccatgtaaa aggacgtttt cctacagcaa tacgttcttt atttatttca tcttctaatt
    13081 catcttgata agcttttgct tgaccgtagg tcatctcttt cgtgaactta tgtttattgg
    13141 catttgcttt gatatgagta gaatcaatat aaaggttgtc ttccgtaacg aagccagctt
    13201 ttacagcttg ttctagaata tgagagaaaa tatcttcaaa taaagttgtt tctctaaatc
    13261 ttcgaacata gtttttacca aaagtagaga agtgtgggat aggatcctcg aatgaataac
    13321 ctaaaaacca acggtaagca acatttgttt caacgtcttt aattgtctgt ctcatcgaac
    13381 ggataccaaa taaatattgg ataaatacta atttaattaa gaccactgga tcaatactgg
    13441 gacgaccata ggttgaatac gtagattcta caatgggata aatgaaatca aactgaatcg
    13501 cagcatcaat ttttcgaact aagtgatctt taggtacaag atcatcaatt gttaacatag
    13561 caaattgatt tctaccttca atcgattgtt ttctcatcat tagaatcac
//
LOCUS       DAHEYX010000058        13597 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHEYX010000058 DAHEYX010000000
VERSION     DAHEYX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.059"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="MB219_001190"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="MB219_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCC8426918.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="MB219_001191"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="MB219_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCC8426919.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="MB219_001192"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="MB219_001192"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCC8426920.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="MB219_001193"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="MB219_001193"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCC8426921.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="MB219_001194"
     CDS             6047..6346
                     /locus_tag="MB219_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCC8426922.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="MB219_001195"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="MB219_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCC8426923.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="MB219_001196"
     CDS             6934..7170
                     /locus_tag="MB219_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCC8426924.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="MB219_001197"
     CDS             7313..9286
                     /locus_tag="MB219_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCC8426925.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="MB219_001198"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="MB219_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCC8426926.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="MB219_001199"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="MB219_001199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCC8426927.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12165..13457
                     /locus_tag="MB219_001200"
     CDS             12165..13457
                     /locus_tag="MB219_001200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCC8426928.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4528 a         2465 c         2994 g         3610 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc
     4441 tttgaaaaag aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa
     4561 gcgacgatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggttagag gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaagatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga
     5821 ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatca gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12001 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12061 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    12121 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    12181 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    12241 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    12301 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    12361 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    12421 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    12481 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    12541 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    12601 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    12661 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    12721 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    12781 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    12841 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    12901 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    12961 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    13021 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    13081 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    13141 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    13201 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    13261 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    13321 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    13381 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    13441 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    13501 gtcgaagtgt ttatctatac gataataata acttgaacac tgtttattca gaataaggga
    13561 ctgtgacaaa aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DAHEYX010000059        13557 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHEYX010000059 DAHEYX010000000
VERSION     DAHEYX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.060"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            29..1230
                     /locus_tag="MB219_001201"
                     /pseudo
     CDS             29..1230
                     /locus_tag="MB219_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1603..1929
                     /locus_tag="MB219_001202"
     CDS             1603..1929
                     /locus_tag="MB219_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426929.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(2046..2390)
                     /locus_tag="MB219_001203"
     CDS             complement(2046..2390)
                     /locus_tag="MB219_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426930.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2466..3713)
                     /locus_tag="MB219_001204"
     CDS             complement(2466..3713)
                     /locus_tag="MB219_001204"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCC8426931.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(3710..4516)
                     /gene="proB"
                     /locus_tag="MB219_001205"
     CDS             complement(3710..4516)
                     /gene="proB"
                     /locus_tag="MB219_001205"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCC8426932.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            4746..5273
                     /locus_tag="MB219_001206"
     CDS             4746..5273
                     /locus_tag="MB219_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCC8426933.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            5336..6706
                     /gene="radA"
                     /locus_tag="MB219_001207"
     CDS             5336..6706
                     /gene="radA"
                     /locus_tag="MB219_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCC8426934.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            6740..7897
                     /locus_tag="MB219_001208"
     CDS             6740..7897
                     /locus_tag="MB219_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCC8426935.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            8067..9527
                     /locus_tag="MB219_001209"
     CDS             8067..9527
                     /locus_tag="MB219_001209"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCC8426936.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    9524..9763
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9849..10385
                     /gene="cysE"
                     /locus_tag="MB219_001210"
     CDS             9849..10385
                     /gene="cysE"
                     /locus_tag="MB219_001210"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCC8426937.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            10382..11794
                     /locus_tag="MB219_001211"
     CDS             10382..11794
                     /locus_tag="MB219_001211"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCC8426938.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            11791..12186
                     /locus_tag="MB219_001212"
     CDS             11791..12186
                     /locus_tag="MB219_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCC8426939.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            12179..13039
                     /gene="rlmB"
                     /locus_tag="MB219_001213"
     CDS             12179..13039
                     /gene="rlmB"
                     /locus_tag="MB219_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCC8426940.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            13043..>13557
                     /locus_tag="MB219_001214"
     CDS             13043..>13557
                     /locus_tag="MB219_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCC8426941.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDL"
BASE COUNT         4330 a         2518 c         3008 g         3701 t
ORIGIN      
        1 catgtatgat gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca
       61 agaacaaaca aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg
      121 tatgatcgct tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca
      181 gttaaacgct acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg
      241 tgtggtgatg ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aacattgatg
      301 acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg gaatatcgga
      361 atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt ctctactgct
      421 ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat gatgtatgaa
      481 gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt gggcagtggt
      541 tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt tatagcccta
      601 gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg ggaaggtgtc
      661 aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt atataatttc
      721 ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc tgaattagaa
      781 gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat tttcgttgca
      841 ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt tgtcttattc
      901 attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt accgatcagc
      961 attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac gattgctatg
     1021 gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt ccgctttttc
     1081 ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg tgaaagctat
     1141 gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat ttatcaccgt
     1201 aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa agctgtcctg
     1261 catcaagtca taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttgggc
     1321 ttattaccgc agatgcaagc ttttgaacgc cgtttaccaa gaggttgtga gactgtcgtt
     1381 taactctgaa tcacaaggaa caatttcaaa aaacagtttc tcatgtttgt tgaatattgt
     1441 gacttatgat tgaggagttg gcagaagaac accatttatt cggaaaaagg ggatgtgaca
     1501 cgatttttgt cacattccct ttttttctca aaataatgaa actggaaaat tttcagtagg
     1561 taagctaaaa ttcatttaag aaaaagaatg gggggataat agatgctgga agaatatttt
     1621 caggaagtcg ggttgacact cgttgaatta cccattgaat tttcagatga tcggttgatt
     1681 tatcgtggct atatcaaaga attgccaata atcaatgcgg aaggtgaaac tagaaaagcg
     1741 atgcagcaaa aaatagctgg tatgtatcag gtgtatcggg aacagctact ggctgaatca
     1801 gcggaagaag aaaaagaaaa aacgatgaac ttgtccatgg atgagctgct tcgttattat
     1861 gatggcgaaa catttgacgg attttcgctt cttaacgaag aggaaacaga tttaccaggc
     1921 agctggtagg cgaacgccgt ttatatggaa aaagaggctg tgacaaaatc tttgtcacag
     1981 cctctaaaaa gtccggcagt tttttgccga tcttcaaggc actctactta tcgtacggat
     2041 attttttagc ggaaagtccg attcaagtgg tttttattca gctgatattt ataagataag
     2101 tgaaaaatcc cagcaatcag tagcgtaatc gtataagcga ttgaatgatc gaaaaaagaa
     2161 tagtaattga ttgtcgctaa taagcataat gcaaccatgt aactaatgtg caagcgtgta
     2221 accagccctt tttgcccgat tttgtcaaaa tactgcgacc atttttcccg ttgactccct
     2281 atgcttcgtt ttttgaattg aaagaaaagg gataaaatac cgactcccag aaaagcagcg
     2341 atattcagag aaataaggta aataaaaaaa atcattaaaa tttcaaacat attgacacct
     2401 ttctagttgg atagaacctt tacagaaaaa aggcaaaagg cgggaataat gtttcacacg
     2461 aaacattatt cgcggatttg tccatcgcca taaatgatat attttgtgga agttaattca
     2521 tttaacccca ttggaccacg ggcatgcaac ttttgagtag agataccaat ctctgcacca
     2581 aaaccaaata ccgatccgtc tgtaaaacga gtggaggcat tgacatagac agccgccgcg
     2641 tccacttcat ttaagaaacg ttgactagcc gcataactat ctgtcacgat cgtttctgaa
     2701 tgtttagtat tatgccgatt gatatgtgca attgcttcat ccaaagaatc gaccactttg
     2761 acagcaagga tataatctag atattcagta tcccaatctg tgtcttctgc taaagcagcc
     2821 gtctctagat attctaacgc acgagcatcc gcacgtaaag agaccccgtg ttctaccagt
     2881 tctttttcaa tagctggcaa gaaaaaaggt gcgatctcag aatggatcag caatgtttct
     2941 gccgcgttac agacagatgg acgggaagct tttgcattga cgatgatgtt cactgccata
     3001 gataactgtg cttctttatc gatatatacg tgattatttc ctgtacctgt ttcaatgata
     3061 ggtaccgttg cctgttcttt gactcgttgg attaattttg ctccgcctcg aggaatcagc
     3121 acatctaagt attctgtcaa tcgcatcaac tgtcctgcag tttcatgaga agtatcatcg
     3181 acaaactgga tggcataagg agattcgcca gcactgacca gtacttgctg caataattgg
     3241 actaataatt gattcgagaa gaatgcttct ttgccaccgc gaaggataac ggcattccct
     3301 gatttgaatg ctagactagc tgcatctgtc gtgacatttg gacgagattc gtaaatgatg
     3361 ccggtcacgc ctagtggcac gcgctttttg ccgattctca atccgtcggt gttcgtccac
     3421 atctcatcta cttttccaat aggatcgtct aacgaagcaa ttttttcgat ctctgtagcc
     3481 atggcattga ttcgctcagg tgtcagctgc aagcggtctt gcatcgtttc actgatattg
     3541 ttttcgactg ctttttctaa gtctttatga tttgcttcaa tgatcgcttg ttgattcttt
     3601 ttgatggttt gtgccatttg aacaagtagc tcatttttgc ggtttgtagg cataagtgct
     3661 agttgttgcg cgctatcttt tgcctgttgt cctaattgca tcaagtcagt catttcaagt
     3721 cctccttaaa taaagtacct acttcttttc ctgcaagaat atcaaaaata attgccggat
     3781 tttttccatt tgctaaaatc attgcgctgt ttgcttcaaa tatcctagaa gcggctttta
     3841 atttactctg cataccgcct gttccaaatg tgctaccagc accacctgct tgtgccagaa
     3901 tcttttcatc gattttatcg atatagcgat acatctgagc ctcgggattt gtagtcggat
     3961 tatctgaata aaacccgtcg atatcagaaa ggacgatcaa aaggtctgcc gcgacaagtt
     4021 ttgccatgat ggctgacagc tgatcattat cgccaaattt tgtcagatga tctaattcat
     4081 ccactgctac agtatcgttt tcgttcacga tagggatcgt gtccataatc aaaagctgtt
     4141 ccaatgtatt cctcacattt ttacgacttt caggataatc taccacgtct cttgtcacga
     4201 ggatctgtgc cgtctgttga ttatatacac tgaatctttg attatagata ttcatcagtt
     4261 cggcttgacc aacagccgcc gcagcttgct gttcaggaat caaagtcgga cgttctgaca
     4321 aatgtaattt atgcatacca acgcctacag ctcctgatga gactaagatc acttcctttc
     4381 cccgatttct aagatcagaa aggacaaaag ctaactgatc gattgccgca agattgatat
     4441 ttccattggg ataaatcaat gtacttgttc ctactttgac aataattcgt tttgcatttt
     4501 taatttcttt acgcatgtga cacctcaatc aaatatccac tcattaattt cgttctattc
     4561 tacttgtttt gacgagtaaa gtcatgtaaa aaatagtttt tttgtacaac ttgcagtcgt
     4621 ttggaaaaag aacgaattaa tggtttactt taagatagca gaatttcttg aagaatttgt
     4681 aaagaaagtc ttttacagcc tcggatatgt tagaatagga gtagtttatt tagggaggaa
     4741 aaccggtgag aaaaagagga tttgaagtta tttctaaata taaagatcaa aatatcaatc
     4801 taccgcagcg cgctacacat catgctgctg gctatgattt tgaagcagca gaagaggtcg
     4861 tgattccaag tatctggaaa gcaggctgga aaaaggggtt ggaaacagtc gcagatggat
     4921 tgaaaaaaga tgtatcagaa aatgaaaaac aattgaaacc aaccctcgtg cctactggaa
     4981 tcaaagctta catgggagaa gatgaatttt tgcagcttgc taaccgttca agcaatccgt
     5041 tgaagcggtt tttagtgttg acaaacggag taggtgtgat tgacagtgat tattacaata
     5101 atgaggcaaa tgaaggccat atcatgttcc aattcaccaa ctttggtttc aaggatgtag
     5161 tgatcaaaaa aggcgagcgt atcggccaag ggatcttttt gccgttttta aaagcagatc
     5221 aagatcaaac gactgctcaa cgtcaaggcg gatttggttc ttccagtaaa taaaacgtta
     5281 aagatactta agcttgattt aatgtctcat gattaggata aaaggaggcg gtacaatggc
     5341 aaaaaaatca aaagtacaat ttgtttgcca aaattgcggg tatgtgtcgc ctaagttctt
     5401 gggacgctgc ccaaattgcg gaaaatggaa tacgatggtc gaagaaatcg aacaagatac
     5461 aacggaccgc agaactcgga caagtttgac aggagaaaaa gcaaaaccaa caaaaatcgc
     5521 agacgtcgtc cctaaaaaag aacctcggat caaaacaaaa ttagaagaac tgaaccgtgt
     5581 gttaggcgga ggtgtggtgc cagggtctat ggtcttgatt ggtggcgatc caggtatcgg
     5641 gaaatccact ttgctgttac aagtttctca gcaattagca gccataggcg gaaaagtctt
     5701 atatgtatcc ggcgaagaaa gcgcagagca gataaaatta cgcgcggaac gcctcggaag
     5761 tatcaacaca gaattttatc tatacgctga aacagatatg aacgaaatca gtcgtgccat
     5821 cgaacatatc tcccctgatt atgttatcat tgattccatc caaacgatga cgcagccaga
     5881 catcacaagt gtggcaggaa gtgtcagcca agtaagagaa accactgcag aactactcaa
     5941 aatcgctaaa acaaatggga tagccatttt tatcgtcggt catgttacca aagaaggctc
     6001 gatcgccggc ccaagaatgt tagaacatat ggtggatacg gtgttatatt ttgaaggtga
     6061 acaacatcat agttttcgga ttttgcgagc agtaaaaaac cgatttggct caacgaatga
     6121 gattgggatt tttgaaatgc atgagcatgg attggaagaa gtcgctaatc cttcacagat
     6181 tttcttagaa gaacgcctag acggtgcaac tggctctgct atcgttgtgg caatggaagg
     6241 gacacgtcca attttggtag agatccaagc attagtcaca cctactatgt tcggaaatgc
     6301 caaacgaaca acaacaggtc ttgatttcaa tcgggtatct ctgatcatgg cagtgctgga
     6361 aaaaagagct gggttgcttc ttcagaatca agatgcttac ttaaaagctg caggtggggt
     6421 aaaattaaat gaaccagcga ttgatttagc tatcgctgtc agtattgctt caagctataa
     6481 agaaaaagga acacagccaa cggaatgttt catcggtgaa attggactga ctggagaaat
     6541 cagacgtgtc agtcatattg aacaacgagt caaagaagtc caaaaactcg gcttcacaaa
     6601 agtataccta cctaaaaaca acttaggtaa ctgggaagcg ccaaaaggaa tcgagatagt
     6661 tggagtagca actttggctg aaacgttgaa gcgtgtgttc cgctaactaa taaatgattg
     6721 tcgaaacgga ggaaaaacga tgcaaaaacg tgtcattact ttgctgatga tcattgtcgg
     6781 cgcaagcttg ggtatttcat ttttaccctc agcttggcag gcaatcggtc aacagtcaaa
     6841 cggatggctg aataatacat ttaccaacag cctgttaggc gcacttattt tctttttatt
     6901 atctttgctg ttggccaaac atatctcagc tattatcaaa caagtagaac aaaaactaag
     6961 tgaggtcagc ttgacttatc ttctcttcgg tgcgatcggt gcgatcatcg gattgacttt
     7021 aggtgttgtg atctcgaccc ctctttacaa tatggaaatt ccattcgtca atagtgtatt
     7081 gccgatttta ctgatgatca tcttaggcta tctgggattg agaatgggaa cgacacgaat
     7141 cgaagaatgg aagaaagttt ttggttcacg aagcaaaaaa atcgagcaag aaacagctag
     7201 tcaagttgag agtcaactgt tagaacgaaa atcaggagaa aatttccata aatataaaat
     7261 cttggataca agcgtgatca ttgacggtcg gatttatgat attacaaaga ctggattctt
     7321 agaaggaacc ttgatgatcc ctaattttgt tctttacgaa ctacaataca tcgcggactc
     7381 aggggatagt ttgaaacgtg tgcgtggtcg tcgcggatta gatatcctaa atgccttgca
     7441 aaaagaagag ggtatatctg tcgaaatgta cgatggagac tttgaagata tcagtgaagt
     7501 agacagcaaa ttgatcaaat tggctaagct tttagatggt gtcattgtaa ccaatgacta
     7561 taacttgaat aaagtctctg aattccaaaa tgtacctgtt cttaatatca atgcgttggc
     7621 aaatgccgta aaaccagtcg tcatccctgg tgaaacgatg aatgttctag tagtaaaagc
     7681 cgggacagaa cgccaacaag gagtagccta ccttgatgac ggcacgatgg ttgtcgtaga
     7741 agatggacag cattacatga atgaacgtat cgaagtcgtt gtaacaagtg cccttcaaac
     7801 agctgctgga cgcatgatct ttgctaaacc tgcacatgca ggacgaggaa tcgacaataa
     7861 aaaagagaca gagaaaaaga ctaaacaatc aaaataaaaa agaaattcag ccaactggta
     7921 aggaaattca accagttggc tttactattt accttagaaa aggtaaaatt tgtgctgagt
     7981 ggctttaaat aaatgatatt tgagaagccg taactgacgg aatttttgct gttagtcaaa
     8041 gtctagtttg aaagaagagg aagaaaatga cgaaagtacg cgtacgctat gcaccaagtc
     8101 caactgggca cctgcatatt ggaaatgcaa gaactgcatt attcaattat ttgtttgccc
     8161 gccacaatga tggagacttt attattcgta ttgaggatac tgaccaaaaa cgtaatatcg
     8221 aagatggcga aaaaagccag ttggaaaacc ttgcttggtt gggaatgaat tgggatgaat
     8281 ctcctgaaaa tcctggagaa tacggccctt accgtcaatc tgaacgtaaa gaaatctatc
     8341 aaccgctgat tgatcagctg cttgctagca atcgagcata caaatgctat tgcacagaag
     8401 aagaattaga agcagaacgg gaagcacaac gcgcaagagg agaaatgcct cattacgcag
     8461 gaacatgtgc gaacttgaca cctgaagaac aagcagaaaa agaagcacaa ggtctagaat
     8521 ctgttgtacg tttccgtgtg cctcgcaata cagaatacgc atttacagat atggtgaaag
     8581 gcgctatttc gttcgaatcc gataatatcg gtggcgactt tgtcatacaa aaacgcgatg
     8641 gtatgccaac ttataatttt gctgttgcgg tcgatgatca tttgatgaaa atcactcacg
     8701 ttttacgtgg agatgaccat attgccaata caccaaaaca actgatgatc tatgaagcat
     8761 ttggctggac accaccaacc ttcggacata tgacattgat catcaacagt gaaacaggta
     8821 aaaaactaag caaacgtgac gaatcaatct tgcaatttat cgaacaatat cgcgaattag
     8881 gctatctacc agatgcaatg ttcaacttta ttgcattatt gggctggtca cctgtgggcg
     8941 aagacgaaat cttcgaacac caagaattga tcaaaatgtt cgatccaaaa cgcctaagta
     9001 aatcacccgc agctttcgat gcgaaaaaac tgcaatgggt aaacaaccat tacatcaaag
     9061 caatggatct agatgcattt actgacatgt gtctgcctta tctaatcgct gatggacgtg
     9121 tagcagaaaa cccagatgaa aagacaatcg aatgggtgaa gaagatcgtt agcctgtatc
     9181 aacctcaaat gagctatgct gcagaaatcg ttgatctgtc tacgctattt ttcaatgaac
     9241 atcctgtatt gaatgaagca gcaaaagaag tacttgctgg tgaaacagtt cctactgttc
     9301 tccacgcatt caaagcacag ctagaacaaa tggaagtagt agatgctcca actgtcaaag
     9361 ctgccatcaa agctgttcaa aaagaaactg gcgtaaaagg aaaaaatctt ttcatgccaa
     9421 tccgtgtagc tgtatcagga caaatgcatg gtccagaatt gcctgaaacg atcgaattgc
     9481 ttggtcgtga aaaatcatta gcacatttaa atcaagtact gaactaaaca cgatgaacag
     9541 aaaaaagtaa gcagctgatc attacaagag agataccggg tggtgcaagg tatcaatgaa
     9601 tggttgccga aatgcctctg ggagtggtct tggcgaaaca cagaaaacag gaaaaactgt
     9661 tttataggaa acaaagtagc caagaacgta taacaggcgt tagctgtcag agataatcat
     9721 tgattatgaa gaaaagtgga accacgcgga aagcgtcttt tgataaggaa acttatttaa
     9781 aaggcgcttc ttttctttat aagaagatga tttcctacat aaaattctta cgaaaggagt
     9841 ggaaagtcat gagttggttt acccgagcga ttgatgcagc aaagagaaac gatccggctg
     9901 cacgttcgac agctgagatc gttttgacat accctggttt tcatgccttg ttttggcatc
     9961 gtttctctca ttttttatat cagcaccggc tctttttatt ggcgaagatc aatgctcaat
    10021 tctggcgttt cctaactggt gttgagatcc atccaggagc aacgattgga actggcgtgt
    10081 tcatcgacca tggaatggga atcgtcattg gcgaaaccgc agaaatcgag gacgatgtga
    10141 ttttattcca tggcgtaaca ttaggcggca caggaaaaga aacgggtaaa agacacccaa
    10201 ctgttaaaca aggagccatg ctctctgcga atgcgcagat attaggaccg gtaacgatcg
    10261 gaaaaaacgc caaaatcggt gcaggtgccg ttgtgttgaa agatgttcca gacgacgcta
    10321 cagcggtagg agtaccagca aaagtcgttc gaatcaaagg agaaaaagtg aggagatcag
    10381 aatgataaaa atctacaata cattgaccag aaaaaaagaa gaattcatcc ctatacaaaa
    10441 gggacatgtc ggtatgtatg tctgcggacc gactgtgtat aattatatcc atatcggaaa
    10501 tgctcgtagc accattgctt tcgatacaat tcgacgctat tttgaatatc gaggctataa
    10561 agtcgatttc gtatcgaatt tcacggatgt agacgataaa atcatccgta cagcaaacga
    10621 attaggtatt acagcaccag aagttgctga tcgtttcatc cgagcatttg aagaagatac
    10681 gaaagcgttg aatgtcgagc ctgctacact gcatccaaga gtcatggatc atatgccaga
    10741 tattttagca tttattcaag tgttggttga aaaaggctat gcttatgaat cttcaggtga
    10801 tgtgtattac cgtacccgta aatttgaaaa ttatgggaaa ttaagtgacc aatccatcga
    10861 tgaactggaa gtcggagcaa gtcagcgtac aggggaagaa cagcagcaaa aagaagaccc
    10921 attagatttc gctttatgga aacaagcaaa acctggcgag atttcttggg attcaccatg
    10981 gggcaaagga cgtcccggtt ggcatattga atgttccgtt atggcaacaa aacatttagg
    11041 agaaacgatc gatatccatg gtggcgggca agatttagaa tttcctcatc atgaaaatga
    11101 aatcgcacaa agtgaagcga aaacagggaa aaaattcgct aattactgga tgcacaatgg
    11161 ctatgtaacg attggcgaag acgatgaaaa aatgagtaaa tctctaggga atttcatcac
    11221 tgtacatgaa atgatcaaaa acgttgatcc acaagtcttg cgtttcttca tggcgaccac
    11281 acaataccgt cgtccgatcc gctacagtga aacgacgatg aaagaagcag ccaccaattt
    11341 acaacgactg aagaatacgt ttgacaattt gaaattccgc caagaaactg cctcagagaa
    11401 tttggcagat gatgcggata aaatcgcttt gttagaaaat ttagaacagc gattcatcga
    11461 agaaatggac gatgatttta atgcagcaaa tgggatcaca gtggtctatg agcttgctaa
    11521 gtggctgaac acttatctag accagccgaa agtttctgcc gctgtcttgg cacaaggaga
    11581 agctttattc tcgcaatggc tttcgatctt cggtatttta tttacagcag aagagttatt
    11641 agatgaagag attgaacaat tgatcgaaga acgaaatcaa gcaagaagca atcgtgattt
    11701 tgtccgaagc gacgaaattc gtgatttgtt gaaagaaaaa gggatcttat tagaagatac
    11761 accgcaagga acccgatgga gaagagaagc atgagagatt acacacaatt gaacggttta
    11821 gccttggctt atgttggaga tgcgatttac gaaatctatg ttcgtgacca cctgattgat
    11881 caaggagaaa caaaacccaa taagctgcat cgtgcggcaa cgaattatgt ttctgcgaat
    11941 gcacaagcct ttttgattca gcaaatgctg gaagaacatc ttttgacaga agaagaagaa
    12001 ctttattatc gcagaggaag aaatgctaaa agccatacga gcgcaaaaaa tgctgatgtt
    12061 acgacgtatc gggtcgcaac agggttcgaa gcgctcatgg gctatctgca tttgacaaaa
    12121 caaaccgagc ggatggaaga attgatccgc tggtgtattc agaaagttgg tgaaaaaaat
    12181 gggtaaagaa agaacaaaca aacatcaatc gaaacgtgga tttgaagaca aaaaacaaaa
    12241 tagatattca aataaaaagc aaactggacg aaatcagtca gtttctgatc aaaatgaaga
    12301 agcattagaa gataatttcg tctttgggca ccatgccgca gtagaagcgc tacaacaagg
    12361 gcgagggaat aaactctttc ttcaagaaga ttcacgagga gaaaaagtag aagaactgaa
    12421 agaaatcgca cgtgaacaag ctgtgccagt caaatgggtt cctaaacaaa aattagaaac
    12481 tttgagcgat cacggcgtgc atcaaggagt cgttcttgcg attacacctt atgaatattt
    12541 atcccttgac caactgattg aacgaacaaa agagaaaaaa gaagacccct tctttttgat
    12601 tttggatagc atcgaagatc cacacaattt tgggtcgatt ttacggacag cagacgcaag
    12661 tggcgtcgat ggtgtgatca taccaaaaca ccgagcagtt ggaatcacac ctatcgtaac
    12721 aaagacttcc actggggcag tcgaacatgt ccctgttgca cgcgtgacga atttggtaca
    12781 agctgtgcaa cagctcaaag aaacaggatt ttgggtattt ggcacagata tgaccggcac
    12841 aaacttccaa aaatggaatg ccaaaggatc aattgcttta gtcattggta atgaaggcaa
    12901 aggaatgagt ccaggattga aaaaagaaat ggatgaattg ctgacgatcc cgatgaccgg
    12961 gcacgtccag agcttaaatg ccagtgttgc tgctggtttg cttatgtatc aggctttttc
    13021 ccaacgtcag ggggagtaga ccatgaaaaa acagctgttg attgtagacg gctacaacat
    13081 gattggttcg tggccggaac ttgtccagct taaaaagcag gacaaaatgg cggatgcaag
    13141 agaagctctt ttacatcgtt tatcaaatta tgcgaaatat gaaggaatgg aagtcatcgt
    13201 tgtcttcgat gcccaactag ttccgggcat ccaacaaaat tataagaaat accagttgga
    13261 tgttgttttt acgaaagaag acgaaacggc tgacagttat atcgagagaa tcgctggtca
    13321 gctgaatgac cgtttgacac aagtgacagt ggcgacaagc gatttagcag aacagtggat
    13381 gatcttttcc caaggagcat tacgaacttc tgcttatgaa ttatataaga cgataaaaaa
    13441 aacggaaaaa acaatagccc aagaaaataa agatatgcat tttattgatt tccgacgtaa
    13501 ttctccatgg aatgaagagc agttaaaaaa attgtcccaa aaattggacg atttaat
//
LOCUS       DAHEYX010000060        13425 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHEYX010000060 DAHEYX010000000
VERSION     DAHEYX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.061"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="MB219_001215"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="MB219_001215"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCC8426942.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="MB219_001216"
     CDS             951..1256
                     /locus_tag="MB219_001216"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCC8426943.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="MB219_001217"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="MB219_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCC8426944.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="MB219_001218"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="MB219_001218"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCC8426945.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="MB219_001219"
     CDS             4416..5354
                     /locus_tag="MB219_001219"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCC8426946.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="MB219_001220"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="MB219_001220"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCC8426947.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="MB219_001221"
     CDS             6725..7465
                     /locus_tag="MB219_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCC8426948.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="MB219_001222"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="MB219_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCC8426949.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="MB219_001223"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="MB219_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCC8426950.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="MB219_001224"
     CDS             10608..11258
                     /locus_tag="MB219_001224"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCC8426951.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="MB219_001225"
     CDS             11259..11897
                     /locus_tag="MB219_001225"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCC8426952.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="MB219_001226"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="MB219_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCC8426953.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="MB219_001227"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="MB219_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCC8426954.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT         4436 a         2480 c         2965 g         3544 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttctt
//
LOCUS       DAHEYX010000061        13420 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHEYX010000061 DAHEYX010000000
VERSION     DAHEYX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.062"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..905)
                     /locus_tag="MB219_001228"
     CDS             complement(<1..905)
                     /locus_tag="MB219_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCC8426955.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSK"
     gene            1333..1896
                     /gene="efp"
                     /locus_tag="MB219_001229"
     CDS             1333..1896
                     /gene="efp"
                     /locus_tag="MB219_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCC8426956.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            1997..2167
                     /locus_tag="MB219_001230"
     CDS             1997..2167
                     /locus_tag="MB219_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426957.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2382..3641
                     /gene="lysA"
                     /locus_tag="MB219_001231"
     CDS             2382..3641
                     /gene="lysA"
                     /locus_tag="MB219_001231"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCC8426958.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3628..4428
                     /gene="dapF"
                     /locus_tag="MB219_001232"
     CDS             3628..4428
                     /gene="dapF"
                     /locus_tag="MB219_001232"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCC8426959.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4431..5312
                     /locus_tag="MB219_001233"
     CDS             4431..5312
                     /locus_tag="MB219_001233"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCC8426960.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5309..6025
                     /locus_tag="MB219_001234"
     CDS             5309..6025
                     /locus_tag="MB219_001234"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCC8426961.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6031..6726
                     /gene="dapD"
                     /locus_tag="MB219_001235"
     CDS             6031..6726
                     /gene="dapD"
                     /locus_tag="MB219_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCC8426962.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6742..7743
                     /locus_tag="MB219_001236"
     CDS             6742..7743
                     /locus_tag="MB219_001236"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCC8426963.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7740..9101
                     /locus_tag="MB219_001237"
     CDS             7740..9101
                     /locus_tag="MB219_001237"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCC8426964.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9061..10212
                     /locus_tag="MB219_001238"
     CDS             9061..10212
                     /locus_tag="MB219_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCC8426965.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10187..11356
                     /locus_tag="MB219_001239"
     CDS             10187..11356
                     /locus_tag="MB219_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCC8426966.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11531..12910
                     /locus_tag="MB219_001240"
     CDS             11531..12910
                     /locus_tag="MB219_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCC8426967.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13191..13420
                     /locus_tag="MB219_001241"
                     /pseudo
     CDS             13191..13420
                     /locus_tag="MB219_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4481 a         2340 c         2920 g         3679 t
ORIGIN      
        1 ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt
       61 ggtaaacgac gaatttctct taatgctgct gcaaaagccg caccactcga acttccaact
      121 aatagtcctt gttctcgtgc taactgccta gtatggttaa acccttctac atcagagatt
      181 gtctcgatct gattgattga aaggggagaa agaaacggcg gaatgaattc tacaccgata
      241 ccctcaattt cgtgaggggc tggatcacca ccatttaaca cagagccctc tggttctaca
      301 ccgattattc gaatgtcggg atatttttct ttcaaaaagg tagaagttcc agcaaaggtt
      361 ccaccactcc caattcctgc tacaaaacta tggatatttt ctttgatttc atgaaagatt
      421 tctggcccta acgtatgata gtaagcagca ggattatctt tattttcaaa ttgaagaggc
      481 agatagctgt ttgatatctc ctctgcaagt tttttacttt tgctgatggc accgatgatt
      541 ccttgctcac taggagaatg gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt
      601 tctaagctaa acttctctgg aacaacaaaa accgttctta atttgtatgt gagtgcggcc
      661 agagcgatcc caattcctgt atttccggca gttggttcga tgatcgtcgt tttttcattg
      721 atttttcctt gggcaaatcc ttctttgatc aaataactgc ccaatctatc tttgatactt
      781 cctccaggat ttaaatattc tagttttgca taaatagcag aacctttagg catagaatag
      841 tgctgattgt caaagcgata aagcggtgtg gaaccaatag cttctgtaat tgtcgagata
      901 atcatatggg cctcctaaaa aaagataata aaaaaaggag ctgactatta aatcagctcc
      961 ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa gttacctggt actcattcta
     1021 tgaagggcaa ttaatagaca tgacaaatag tatgctgctt ttatgcacca tacaacagca
     1081 acgattcaat tgtttttggt tgctgactgt catgtgtatc cctccaagta aaatttttat
     1141 taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat atttcgatag
     1201 catcgtttac ccgtgtttat ttttctgaca aataggcatg agaccttaaa agatagagaa
     1261 tactctagtt ttctggccgg ttgtctgata caataaagtt gattatgata aagaaaagaa
     1321 ggagcaaaaa taatgatctc agcaagtgat ttaagagccg gtatgacttt cgtacaagac
     1381 ggcaaactga ttaaagttct ggatgcaagc caccataaac ctggtaaagg aaatacagtt
     1441 atgcgtatga aactgaaaga tgtccgttca ggtgcaacat acgacacaac gttcagacca
     1501 gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg tacaatattt gtacacgatg
     1561 gatgatacag ctttcttcat ggatttggaa acatatgagc aatatgaaat tcctgtagaa
     1621 acagtagaag aagaaatgaa atatatccta gaaaacatgg aagtaaaaat ccaattcttc
     1681 ggtagcgaag tcatcggcgt tcaattacca acaactgtcg tattacgtgt tgtggaaaca
     1741 caaccttcca tcaaaggtgc aacagtcact ggttcaggta aaccagcgac aatggaaact
     1801 ggcttagtag tcaatgttcc tgacttcatc gaaacagatg aattgttgga aatcaataca
     1861 gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg ctaacggatt gataagaatt
     1921 tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga tctgcctttc aagaggtttt
     1981 ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata ttttgaaaga
     2041 tgaatggcaa aagctagaga aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat
     2101 tcataaaatt gaaaatatcc gaactattcc gaactatatt gaagcgaaaa tattttttca
     2161 tcactaaata gtgttgaatt tatgttttta tgtataaata gcttctaata gttcgggtat
     2221 tttgttttta taaccatttt tattcttatt tttaaaaaaa tatttcgaaa tatactagac
     2281 aattcagaaa attttgttta atattattcc aataaaattc aaatctttat caaatgaaat
     2341 tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg
     2401 gcggagtcac agcaagcaca ttggcagaaa agtataagac acctctgtat gtgtatgatg
     2461 aagaaaagat gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg
     2521 atacaaaagt gctttacgcc tctaaagcat tccaaacgat tgagatgctg catttagttc
     2581 gtgaatatgg attcgggcta gatgttgtca gtggtgggga aatctatacc gctttgcaag
     2641 ctgattttcc tagagatact atctacttcc atggtaacaa caagacgcca gaggaactga
     2701 tctatgcact tgaaaatgat ttgttgcata ttgttgccga taatttgatg gaagttgaat
     2761 tattagcaga attaagccaa cgctatttaa aaaaaatctg tatcatgctt cggttgaatg
     2821 tcggagtaga agcacatact catgaatata tcgtcactgc gcatattgat tcaaaattcg
     2881 gcatgagcta tgaaagtaaa gagtgccaac aagcccttca gttgataaaa gaaagcagct
     2941 atctagagtt agaaggattt catgctcata tcgggtcaca aatttttgat gttactgctt
     3001 ggttagctga aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa cctctttcct
     3061 tgaatcttgg cggagggttt gggattcgtt atacacaaaa tgatcagccg atcccagtcg
     3121 aagaggcact aaaaaaactg attgaatata cggaagaagc tttggaaaaa cgaaaactga
     3181 ccattcgtca attactgatc gaaccgggta gaagtattgt tggagaagct ggtacaacac
     3241 tatacacgat tggtttcatc aaaaagacac cacataaaaa atattacttt gtagatggcg
     3301 ggatgacaga taatatccgt ccagctctgt atcaggcaga atacgattgt gatttagcag
     3361 caaaactgtc ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg tgtgaatcag
     3421 gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga atcgggtgat cttttagctg
     3481 tttattccac gggagcttat ggatactcga tgagcagcaa ctacaatcga gcgacacgac
     3541 cagcagttgt ttttgtcaat aatggccaat caagactgat tgttcgcaga caagcatttt
     3601 ctgatctact aaggggggaa gtttcctatg atccttcata aatataatgg ctgcggaaat
     3661 gattttatct tattagatta cgcagaagac acagattata gtcaattagc cgcttcttta
     3721 tgcacaaaag aaaaatatga tatagacgga ttgatcgccg taaaaatcga tccgttggaa
     3781 atgatctatt acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt
     3841 tttgctcgat acgtaaatga tcagaacatg atgaatgaac aggaattcaa cgtagagaca
     3901 ttagcaggca caagaaaaat tacggtgttg gatccctcac ctttttattg cagcgtagat
     3961 atgggagtgc cagatgattc tccggaaaaa atcaaagcta atcaaactta tccaataaaa
     4021 aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg
     4081 attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa
     4141 ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc
     4201 aatgaacaag aattgatcgt ccgtacctat gaaagaggag taggttggac acttgcttgc
     4261 ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat
     4321 caggtagttg ttcacttgga acaaggagac ttgatgatct ctggtgagga tgcgatacaa
     4381 atggctggcc cagcagttca tgaatggaca agaatattgg aggaatgaag atgataaaag
     4441 gttcgatcgt ggcgctgatc acgccgatga atgaagaggg atcagtagat tatgctggtt
     4501 tggaaaaatt gatccaattc catttagatg aacaaacaga tggattatta gttttaggta
     4561 caacaggaga atcttctacg ctcacacaat ctgaggagga gcagatcctt caactgacag
     4621 taaagaaagt ggctggccgt gtgccggtga ttgcaggagc tggaacgaat aatacgaaag
     4681 aaacgattga aaaagcgaag cattttgctt ctttgggggc agacgcactt ttggtcatca
     4741 ccccttacta caacaaaaca agtgatgcag gactagctgc tcattttaca gcaattgcag
     4801 aagcctcacc gattccgctt attttgtata atgttccttc tagaacaggc atgtcgattc
     4861 caatccatgt attagtaaat ttagcagaac atcctaatat cattggtttg aaagaagcct
     4921 caggagatat ggcttatgtg atggatgccg ctcggttgat tggagaagaa ttcttccttt
     4981 acagtggaaa tgatgatttg atcctaccag taatgagtgt aggaggaagc ggtgtaataa
     5041 gtgtatgggc gaatatccaa cctaaaatag tccatgaatt agtaaaagat acgcaggacg
     5101 gaagatggca gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc catgccttgt
     5161 tcagtgagac caatcctatt cctgtcaaag cagccatgtc attattagac ttaccttccg
     5221 gacctctacg cctgccttta gtttctcttt caaaagagaa aaagaaacaa ttagctcagc
     5281 ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga ctgattggca gtggacagat
     5341 gggaaataga gtcgaagaag ttgcacaaga aagaggagat acggtccttc ttttacggac
     5401 ggcacctaaa gaagcaacta cgcttgcttt tacggaactg ccagaactgc ttattgattt
     5461 ttctcatcca gataatttag agaaaatcct ttcgtatagc ttatcatatc aacttccgtt
     5521 agtcattggc acgacaggtt atacacaatc acagttccaa gaaataaaag aacatgccaa
     5581 acatgtacct gtgatgtata gtgctaattt ttcgttcggc atcatggtga tgaatcaact
     5641 aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc gaactgattg aaaaacatca
     5701 tagtcgtaaa aaagatgcac catctggtac tgcaaatatg ctgcttcaaa caatccaaga
     5761 agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa aaaagagaag aaaaccaaat
     5821 tggtgtacac agtattcgtg caggttcttt acctggtgaa catgatgtat tgtttgctgc
     5881 tactgatgaa atatttacga tcaaacatga atctttttct aatcgaatct ttgctgcagg
     5941 cgcagtagaa gcagcggact ggttgaaaga tcagtcacca ggttattaca aattagaaga
     6001 tttattaatg gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt
     6061 caaacggcat ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa
     6121 tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg tgactatgag
     6181 acgattgctc cggttttaga agctgaatca gaaaaaatca ttgaaatcga aatcgaaact
     6241 gctgcaagat acagcgcagt tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa
     6301 ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca atgccgtgat catgatggga
     6361 gcgattatca atattggtgc agtaatcggt gaaaacacga tgattgatat gggtgctgtc
     6421 ttaggtggac gagcgacagt gggcaaaaac tgccatattg gtgcaggagc tgtcttagca
     6481 ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag aagacggcgt actggtaggt
     6541 gcgaatgcag tgattgttga aggtgtccat atcggaaaag atgcagtcgt ggcagcagga
     6601 gcagtggttt tagaagatgt ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta
     6661 ttaaaaataa gtgatgatca gacaaaagaa aatacagcat tgattgctgc gttacgcgaa
     6721 ttatagagga gatggagaaa aatgcgcgaa atagatacct gtattgtggg ggcaaccgga
     6781 ttagtcggcc aaacgatgct caaagtgtta gaagaatatg attttccagt aggacgattg
     6841 aaattattag cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa aaagaaacac
     6901 actgtacagg aattgacaga aacttcattt gaaggatatg atttagcgct attttctgca
     6961 ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa gtcagggact atttgtcata
     7021 gataattcct cagcatggcg ggaagattcg gatattccat taatcgttcc agaggtcaat
     7081 ctggaagact atacattgaa tcggctgatt gcaaatccaa attgttcaac cattcaagcg
     7141 gtacttccgc ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa ttattcgacc
     7201 tatcaagcag tttctggttc cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat
     7261 gacgaggaac cagttttgta tccttataat attagccaaa cggtgattcc ggaaatcgat
     7321 cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa tgataaatga gacccgtaag
     7381 attctgcatc agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat
     7441 ggacatggag tctctgtggc agttcaactg caaaaagagt ttacgacgga agaaattaga
     7501 gagtgtctca gccagtttcc agggattcaa ttagaagatg atctagcagc acataaatat
     7561 ccgacgagca ttcttgctag aggaacagat atggtttatg ttgggaggat cagaaaagac
     7621 acttctttag agaacggatt gttgttctat acgactgccg ataacatacg caaaggagcc
     7681 gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga
     7741 tgagtaatac gctgaaagta gcgaaattcg gcggaacatc gatgtctcat gaagagcaat
     7801 tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg ccaagtgatc gttgtttctg
     7861 ctttaggcaa acgtacgtca gatgatgaca aagtaacaga cttactttat ttgatttatg
     7921 cttaccttca gcatcatgtt gattggacac ccctttggaa aaggatttgt gatcgtttca
     7981 ttcaagtaag agatgttctt gccctgcagt tacctattga agatgaacta gcacgcatac
     8041 agaaacaact ggaagacgaa aagataactg aagattattt agtaagtaga ggtgaatatc
     8101 tgactgctca actgatggct gagtatcttg gttaccagtt tattgacgca aaagacctga
     8161 ttttctttga ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag
     8221 catttaaaag atatccgaaa atggttgttc cagggtttta cggggcgaac cctgcaggaa
     8281 aagaaaaatt attgggccgt gggggaagcg acattaccgg cgcgatactg gcgaagatgt
     8341 tgcaagcgga aagttatgag aattggacgg atgtttcagg aatcatgatg gctgatccgc
     8401 gaatcatcga tcatcctaaa atcatcgagg aaatctcttt tagagaactg agagagatgg
     8461 cttatatggg ggctaacgtt cttcatgaag cagctgtttt cccagttcaa gaggctgata
     8521 tccccatcca aataaaaaat accaatgatc ctttcgctca agggacaaaa atatcaaatc
     8581 acgaaataga aaaggaaaat ggactgacag gaatagctgg acgcaaggat tttctttcca
     8641 tcactttatt caaacgtcat atgtccgatg aaatcgggtt tatctggaaa gcaatgagca
     8701 tttttgctaa acatgagata tcaatcgaac atattccttc agggatcgac aatatcggag
     8761 tgatcgtctc agcagaagcg attgccgata agttatttct catcacgaaa gaattaaaag
     8821 aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt agctttgatc tctgtcgttg
     8881 ggggtccgca taaagaattg attggattat ctgggaaagt gttatcgata ttgaacaaat
     8941 tagaaattcg cacatcgatc ttatcacaag gagcacagga gttgaatctg atcatcggtg
     9001 taccgaataa tcaatatgaa acagtagtga agggtatcta tgaagggatg gtgaatacgg
     9061 atgctgaaag aatatcgaat ggcactccat caaattcctg aaatcgggtt tcatgaattc
     9121 aagacaaaaa aatatttata tgaacaagta aaaaacagcg aggccgttat ccatgaaatc
     9181 ggcgaaacag gactactcct ttattttgat aatcagcaag aagaaacgat tgcttttcga
     9241 acagatatcg atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat
     9301 gaaggttata tgcatgcatg tggtcatgac ggacatatgg cgatgctttt agggttgact
     9361 gactttataa ctaaacacat tgaggatctt atttataatg tagtactgat ctatcagcca
     9421 tcagaagaaa tttccggtgg cgcacaaagc gttattcagt caggattgtt ggataaatat
     9481 gaagtacaag caatatttgg ctttcatcta tggcctggac tagctaaggg agaagttttc
     9541 tctcgaccag gacctttgat ggcacaaagc agtgaaacag acattatcat taaaggccag
     9601 tctgcacata ttgcttctag tgaaaaagga atcgatagtc tggaggcagc tgtacgattt
     9661 ttgaagaatg tttatgattt tgaccggcaa ttaccggcag agtggaatca tctaatgaaa
     9721 tttggccaaa tccaaggtgg aacgatccga aatgttttag ctggagaagt tattgtatcc
     9781 ggaagtatcc gttcttacag ttcagatatg caaaattatt tgaagagggg attagaccat
     9841 ttagcagaac agtttcactc tacagctact atttcttttc gctataatga cggctatcca
     9901 gctgtaatca accatagagg cttatatgca gcactgttag attcaaacaa actacatgaa
     9961 ttagatgctc cagttcttca agccgaagat tttggcatat atacgcaaca atatccatgt
    10021 gtatttttct ttttaggtgt cggtgatacc cctgctttac atgatccgac gttcgatttt
    10081 gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt tacagaaaat
    10141 cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag taaataatga tgaatttatc
    10201 caatcgattt agtcctaggc tttctagaat agaagtatca aaaatacgac aattcgatca
    10261 gcaaatatcg tcgataccag atgtgatcaa actcaccctg ggagaaccgg attttccaac
    10321 tcctgaacat gtcaagcaag caggaatagc agcgattgaa gaagactttt ctcactatac
    10381 cggaatgaga ggattagagg aactgcggga agcggcttgt atatttcagc aacagcgtta
    10441 tggattaact tatgatccgc aaacggaagt tttgacgaca gttggtgcaa cggaagcgat
    10501 tgcctctgcg ttgttatctg ttttggaaga aggagacaag gttcttattc cagcgcctgc
    10561 ttattcagga tatcagccat tagtggaact agctggtgct gaattgatcc cgatagatac
    10621 ttctgacaca gggtttgtct gtcagccgga acaatttgaa cgagcatttg aacaatatgg
    10681 atcagctgtc aaagcagtca ttttaaacta tcctaacaat cctactggga cgacattgtc
    10741 tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat ttgttataag
    10801 tgatgaagta tatgctgaat tgacttatag tggaacgcat atgtccatag ctacttatct
    10861 tccagaacaa acaatcgtga taagcgggtt atccaagtca catgcgatga ctggttggcg
    10921 agtgggcttt atttttgctc aaaaagccct tattgacgaa ctaattaaag tccatcaata
    10981 tctggtgact gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg
    11041 ggtgaaagat agcgagaata tgaaagagca atatcaaaca cgaagagact atctggtcca
    11101 gcaattggcg cctttaggat ttgaagtgac ccagccaaac ggtgcatttt acttgttttg
    11161 caaactccca gctgccattc aaactgactc ttggcagttt tgtgtagaat tagcggaaca
    11221 ggcaaaagtt gcgtgtatcc caggaattgc atttggccca gaaggagaag gttatatccg
    11281 gatttcttat gctagcagca tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt
    11341 tttaacagaa cagtgattca tagattttat caaaaaaaca atcgttaatt ttgcggtata
    11401 ttctgcaagg agacgattgt tttttaaatt tagttcaaat gaacgcgttt cttttgattc
    11461 ataactaatg taatgttaag tttaaattgt ttagaatgat tttaaaagaa tattcaagga
    11521 ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg gagggcatca
    11581 atcgatcttg gaactattga cacgatacgg tgaaaatgta gatatcacgc tatttgaagc
    11641 aggagactat atttccttca tgtcatgtgg tatggagctg tatttagaag acaaggtaac
    11701 agatgtaaat gatgtacgta atttcagacc agaaaacttt ccgcaatcca atgttcatat
    11761 tttaaataat cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa cggttactag
    11821 cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa ttgattttga gttctggtgt
    11881 caaaccaaac aatctgccag taccaggagc agatctagaa aatgtctact tgatgcgtgg
    11941 atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc
    12001 tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa gcaagccgaa aagctgggaa
    12061 agaagtcgta ttgcttgatg tgatcgatag acctttaggc acttatttag atgctgagat
    12121 gacagatatt ttagaacaac acctaaatga aaacggtatt gaagttttga caaatgcaaa
    12181 aatcgaagct tttactggta acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat
    12241 cccagcagat ctagtcattc aagctgccgg tgtgaaggca aatacagaat ggttaaaagg
    12301 gatcgtcgac ttagatgaac gtggctggat ccaaacaaat gaatacttgc aaacgaatct
    12361 tccagatgtg tatgcagtag gagatgctac acttgcttat tctatcccag cacgcaaaaa
    12421 aatgccgatc gctttagcga ctgttgcacg tagagaagca cgttatgtag taaaacatct
    12481 ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg cattaagtgt
    12541 ctttgactat cattttgcag caagcggatt gaatagcttt acagcagcaa aagcaggagt
    12601 agatgtacga gtgtcttatt atgaagatac gttacgacca aaatatgtac cagaagaaca
    12661 aggaaatcca aaagtgtcca tccaattatt ctcggatcca atgacccatc aattgctagg
    12721 aggagcagta ttatctacct atgatgttac aggtcaagga aatgtattgg cactagcgat
    12781 ccaaaacaaa ctgactttag aagaactagc agaagctgac ttcttcttcc agccaggctt
    12841 tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa gcgttaggag aagaaccatt
    12901 tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag
    12961 taataagaac agccaagtaa aaacagcttc ttatgttttt aacttggctg gacttattac
    13021 cgcagatgca agcttttgaa caccgtttat tttcaaaaaa gggactgtga cacgatttgt
    13081 gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg
    13141 gttaaatata cttttgaact taaattaaag atcgttcatg attatttgga tggcaaaggc
    13201 gattggaccg taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa attcgggaga
    13261 agcacaagga ttatggctgt ttacggatga ccaatgaact aagaaaccgc ggtttctcta
    13321 tcaataaaaa gaaagttcag cgcttgatca agaagctagg aataaaagta acggcataca
    13381 cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAHEYX010000062        13156 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHEYX010000062 DAHEYX010000000
VERSION     DAHEYX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.063"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            3..740
                     /locus_tag="MB219_001242"
     CDS             3..740
                     /locus_tag="MB219_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCC8426968.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            754..1569
                     /locus_tag="MB219_001243"
     CDS             754..1569
                     /locus_tag="MB219_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCC8426969.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1582..2229
                     /locus_tag="MB219_001244"
     CDS             1582..2229
                     /locus_tag="MB219_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCC8426970.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2245..2901
                     /locus_tag="MB219_001245"
     CDS             2245..2901
                     /locus_tag="MB219_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCC8426971.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3063..3884
                     /gene="racE"
                     /locus_tag="MB219_001246"
     CDS             3063..3884
                     /gene="racE"
                     /locus_tag="MB219_001246"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCC8426972.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3887..5242
                     /gene="rph"
                     /locus_tag="MB219_001247"
     CDS             3887..5242
                     /gene="rph"
                     /locus_tag="MB219_001247"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCC8426973.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5243..5761
                     /locus_tag="MB219_001248"
     CDS             5243..5761
                     /locus_tag="MB219_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCC8426974.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5868..6368
                     /locus_tag="MB219_001249"
     CDS             5868..6368
                     /locus_tag="MB219_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCC8426975.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6648..7568)
                     /locus_tag="MB219_001250"
     CDS             complement(6648..7568)
                     /locus_tag="MB219_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCC8426976.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7733..8746
                     /locus_tag="MB219_001251"
     CDS             7733..8746
                     /locus_tag="MB219_001251"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCC8426977.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8856..9611)
                     /gene="fabI"
                     /locus_tag="MB219_001252"
     CDS             complement(8856..9611)
                     /gene="fabI"
                     /locus_tag="MB219_001252"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCC8426978.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9831..10271
                     /gene="fabZ"
                     /locus_tag="MB219_001253"
     CDS             9831..10271
                     /gene="fabZ"
                     /locus_tag="MB219_001253"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCC8426979.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10386..11147)
                     /locus_tag="MB219_001254"
     CDS             complement(10386..11147)
                     /locus_tag="MB219_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCC8426980.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11336..12430
                     /locus_tag="MB219_001255"
     CDS             11336..12430
                     /locus_tag="MB219_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCC8426981.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12516..>13156)
                     /locus_tag="MB219_001256"
     CDS             complement(12516..>13156)
                     /locus_tag="MB219_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCC8426982.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         4222 a         2280 c         2761 g         3893 t
ORIGIN      
        1 ctatggcaat gattaaattt gatcacgtgg ataaatatta tggcaagttc cacgcattaa
       61 aaaatatcaa tctcgaattc gagaaaggag aagttgtcgt tgtcattggt ccttctggtt
      121 caggaaagag cacgatgctt cgctgtatca atggattaga gacgatctct tcaggtaagc
      181 tgctgattaa tgatactgac ttgcatgata aaaagacaaa actgacagaa gtccgaaaaa
      241 acatcggcat ggttttccaa catttcaatt tgtacccaaa taaaactgtt ctggaaaaca
      301 tcaaattagc accaatcaaa gtgttgaagc aagaccaggc aactgcagtc aaaaacgcgg
      361 agaaattttt gaaaactgtc aatatgttag ataagaaaga ttcttatcct tccatgttat
      421 ccgggggaca acagcaacgg gtggcaatcg ccagaggttt agcgatgaat cctgagatgc
      481 tgctgtttga tgaacctact tctgctttgg atccggaaat gattggtgat gtattagacg
      541 ttatgaaaaa attggctcat gacggtatgt caatgattgt cgtgacacat gaaatgggct
      601 ttgccaaaga agtggcggat cgtgttatct tcatggctga tggagaagta ttggaagaca
      661 gcagaaatgt gagagacttt ttcgaaaatc caaatgaagt ccgagcacag caatttatca
      721 gtaaagttat caatcattga taggaggaaa aagatgcgca ggttaaaaaa aatacgcctg
      781 attttccttt taggcattgc ttttcttgca ctagccggat gtaaaggaaa tgggttggca
      841 aatgaagata ttttagagcg aagcaaatct accaatgaaa tcatctgggg tgtcaagtat
      901 gatactcgat tatttggaat gatggatatt gagagcagaa ctgtacaagg atttgatgta
      961 gatattgcaa aagcaatcac taaaaagatc cttggtgaca atggaaaaac agaatttgtt
     1021 gaagtcactt ctaaaactcg gattccatta ttgaaaaacg gaaatatcga tgcaattatt
     1081 gcaacgatga cgatcacaga tgaacgaaaa aaacaggttg acttttcaga tgtctatttc
     1141 gatgctggcc aagctttgtt ggtaaagaaa ggaagccaaa tcaaaagtgt agatgatttg
     1201 aatgcttcta caacggttct agcagtcaaa ggttctacat cggctgcgaa tatccgacag
     1261 catgcaccag atgcaaaaat tttagaatta gaaaactatg cagaagcatt tactgcttta
     1321 caatctggtc agggagacgc gatgactacg gataatgcga ttttgttagg gattgcagat
     1381 gaaaatccag agtatgagtt agttggtgga acgtttacta atgagcctta cgggattgcg
     1441 atcaacaaag ggcaagaaaa cttcttgaaa gctgttaacc aagcgctaga ggaaatgcat
     1501 gcagatggca catacgataa aatctatcaa aaatggtttc caaatgaaac tgaaggaaaa
     1561 gtagagtaag ggggagaggc aatgagcgaa ttgatacaaa cttatggacc aatctttgtc
     1621 gacgggttca agatcacatt gttttcgagt gtcttagcac ttgtgtttag tttgattatc
     1681 ggaacgttaa tggcaatctt ccaattatca aagaataaag tagtgagcgc cttagcaaag
     1741 gcatatgtag aattttttag aaacattcca ttattaatca tcgtgatgtt tttttatgtt
     1801 gtcgtaccga tttattggtt taatctagat ggattccaag caggtacgat tggattaacg
     1861 atctatacat ctgcttttat tgctgaaaca gtacgttcag ggattcaaac cgtaccaaaa
     1921 ggtcaaacag aagcaggact atcttctggt tttacctatt cagaaatcat gcgttatatt
     1981 gttttaccgc aagcattcaa aatcgttatc ccgccattag gcaaccaatt tatcaacctc
     2041 gtaaaaaact catctatttt agcaatcgta gctggtttag atttgatgta tcaaggagac
     2101 ttgatcgcta gtgaaacatt caataccttc gatacgtata tcatcgttgg gttattttac
     2161 ttagttatca cgctaccgtt atcttactta atggtttatc ttgagaaaaa atgggcggtt
     2221 cgttcataga aggaggggaa atacatggat tttagcggcg ctttttcatg gatgaatatc
     2281 cgttttttat tggaaggatt aaaggttact gttgaagttt cattgttttc gatcattttt
     2341 agtttcttga tcggtggtct gacaggtgtg attcgttttg cgaatatccc tttcttttcc
     2401 aaaatccttg gtgtagtgat tgatattata cggaatcttc cattattatt gatcattttc
     2461 ttcacttact ttgccttacc tcaaatcggc attcagatga acatcttctg gtccgctgtc
     2521 gcagcattaa cgatttttga gtcagctatg ttgtcggaaa ttttccgagc tggactcaat
     2581 gctgttccaa aaggacagat ggaagcagga ttatcaacgg gactgactta tgtagaatcg
     2641 atgcgaacga tcgtgttacc gcaagcattt aaatcaatga tcccggcaat tgtgagccaa
     2701 ttgatttcac tgatcaaaga tacttcatta gcagtaatca tttctttacc tgaactttct
     2761 catcaggcac gtattattta tggacagaac accaattatg tgcttccgat gttcatcatc
     2821 atgacactta tgtacttcgt ggtatgttac gcgctttctc tgctttccgg ttacttggaa
     2881 aagagacggt acagttatta atagatgatt cttttagcgg ttgtggcaaa ctaaagtcac
     2941 aaccgctttt cctctttcca ttcgaaaaaa agagatttcg attgttttaa gcaagtaaga
     3001 gcacttgaaa attcacgcta aaaagtacac aatagggagg atagaattta ttgaaaaaaa
     3061 ggatgatacg attgacagat aatcgcccta tcggatttat tgattcaggt gtcggcggct
     3121 tgactgtagt aaaagaagcc ctgaaacaat taccgaatga aaatatttta tttgtaggag
     3181 acacagcacg ctgcccatat ggccctagac ccgcggaaca ggtaatccag tatacttggg
     3241 aaatgacgga ttatctggtg gagcaaggaa tcaagatgct ggtgatcgcc tgcaataccg
     3301 caactgcggt ggctttagaa gaaatcaaag ctgctctttc tattccagtc atcggtgtga
     3361 tccttcccgg tactagagcg gcagtaaaaa aaacacaaaa taaacaagtt ggcattatcg
     3421 gtacgattgg tacggtaaaa agtcaagctt atgaaaaagc actgaaagag aaagtaccag
     3481 aattgactgt gacaagtctt gcttgtccca aatttgtttc agttgtcgaa agtaatgaat
     3541 accattcatc ggtggcgaaa aaaattgtgg cagaaacatt agctccttta accactaaaa
     3601 aaatcgatac attgattttg ggatgcaccc attatccatt attacgcccc atcattcaaa
     3661 atgtaatggg agaaaatgtt caactgatcg attctggagc agaaacagta ggtgaagtat
     3721 ctatgctgtt agattatttc aatttgagca attcaccgca aaatggtcgg acattatgcc
     3781 agttttatac aactggctct gccaaacttt tcgaggaaat agctgaagac tggcttggaa
     3841 tcggacactt aaatgtagaa catatcgaat tgggaggaaa ataaggatgc gtcatgacgg
     3901 gagaaaagcc caagagcttc gcaaactgga gcttcaaaca aatgttttca aatatccaga
     3961 aggatcggta gtgatccgtt ttggtgatac aacagttatt tgttctgcaa caatagaaaa
     4021 ttctgttccc ccttttttga gagaaacagg aacaggatgg gtgacagcag aatatagtat
     4081 gctgcctcga gcaacgagta cgagaaatcg tagagaaagc agcaaaggga aactttctgg
     4141 tagaacaatg gaaatccagc gtcttattgg tcgttccttg cgtgcagtag tagacttgga
     4201 aaaattagga gaacgtagta tcatcgtaga ctgcgatgtg atccaagcgg atggcggtac
     4261 tagaacagca agtatcactg gggcatttgt ggcattgaaa ttagccattg aaaaactttt
     4321 aagagaaaaa gaattgtcag aagatccaat aaaagaacat cttgctgcag ttagcgtagg
     4381 aatcttacca gatgggactt gcgtcacaga tttggattac caagaagatt ccgcagcact
     4441 tgtagacatg aatcttgtaa tgacagaatc tggaaaattc gtggagattc aaggaactgg
     4501 agaagaagca acgtttgatg gtgaacagtt gaatgaaatg ctcttttttg gaaaaaacgc
     4561 gattgaagat ttgatcaaag agcaaaaaca tgctttgcta acagagtttg ctcaaaatga
     4621 tgagcggata gaagaaacaa aaacaatcat cattgcgaca agaaatccag gaaaagcaga
     4681 agagtttcga aatatgttca aagaagcggg atatcatgta aaaacattac ttgactatcc
     4741 tgaattgcca gatgtggaag aaactggcag cacatttgaa gaaaatgccc gattaaaagc
     4801 agaaacgatt gcacaattgc ttgatcagcc agtcttagca gatgattcag gtttgaaagt
     4861 ggatgcttta ggcggtatgc caggaatcta ttctgcacgt tttgcagggg aacaaaaaag
     4921 tgatgcgggg aataacgcca aattattata tgaattgaca gatgtgccgg atgaaaaaag
     4981 aacagcacaa ttccattgta cgttagtatt cgcggcaccc aaaaaagaca gccttgtagt
     5041 agaagcggaa tggcccggtc gagtagcccg tataccatct ggcgaaaatg gctttgggta
     5101 tgatccttta tttatcccag aaggtaagaa acaaacagca gcagaattat cgagcgaaga
     5161 aaaaaacaag atcagtcatc gggctcaagc aatgaaaaaa ttgagtgccg agtggaaaca
     5221 atggctggaa ggagagagat agatgcggta tttagtagta agtgataatc atggagacag
     5281 agatatcctc gttgacttag tagatcgtta tcgttctgaa gtggatatct ttttccattg
     5341 tggtgattcg gaattagaag cttctgaccc attatgggat acattccaag tggttcaagg
     5401 taattgtgat tatggaccag gattcgaaca gaaaaaagtg atccaaactg gtcaggatac
     5461 agtattcatg actcacggtc atttatcgaa tgtccgattt ggtttgacac agttagcaat
     5521 cgaagctcga gctgcaactg ccgatatggt gttttttggt catacccatc aaataggctg
     5581 tgagatggat cagaaaatcc tgtttttgaa tcctggtagc atttctcagc ctagaggccc
     5641 gatccaaatc ccatcatatg ctcttatcga aagtttagat gaccaaattt ctgttcaata
     5701 ctataaccgt gcccatcagg cagttgaaga gttggccttt tctttttcga gagagcgctg
     5761 aataaaaaaa ctctaatttt taataaaaat aagctttatt ttatgaattt tgcatccggt
     5821 ttcggtaaaa tagtagtgtt gaatgaaata gaaatggagg ccatcagatg attggaccta
     5881 tagtaagaga aatgctgctg gaaaatcagg aaacgttttt gattcctgca gaaaatgtgg
     5941 caaatgttat gtatcatcat ccattgtccc atggtttact tgtgttatcg aaggtcggtt
     6001 attccaaaat tcctgtatta ggaaaagatg atcactttgt aggactagta agtttatcag
     6061 atgtggtcaa taaaatgatt gacttgcaaa caatcagcat ggacccgtta gaaggactaa
     6121 cggttgctga tgtgacagaa acagatgttc cgacaattga tgaaaattgg gaactagaag
     6181 aagtactcca tttgttagtc gattcgcctt ttttaccggt tgtcacagaa gatcggattt
     6241 tcaaaggaat cattacaaga aaagagctgc ttaaagccgt aaacttcatg gtgcatgaat
     6301 tagaacgtcg taatatattg tctccgaaaa tcgaagaatt aaaagaacaa atcgatatcg
     6361 tgagctaaag aacgagaaac taaaagataa tcaaaaaata agtagaaaaa acagcgtacc
     6421 agttttgact ttatcttgaa aaagaggctg ggacaaaagc gtttaactcc aagaaataag
     6481 aaggaattca cgaaaattgc ttttcaaatt tttgtgaatt tcagcttatt tccgaaggag
     6541 ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtgg gacaaaagtg ttctttactt
     6601 ttgtcccacg ctctttttag tgagattcgt tgcatcgttt gtccaaatta ttttccgaca
     6661 gcaattggac tgttcggaat cgtgaaacca gaagctgtca attctttgac ataggagctc
     6721 aagaactctt cttttaaggc atattgtttt ccgttcaatg cgtagcagac cgtacgaatc
     6781 gcaaaattgc tgtttccaag atccactaat ccaaagatag aaggttcagt ttggagctct
     6841 tcttggtatt tttcagctaa tgtacgattt actcgatcaa tgatctcata gattttgtca
     6901 tatccttctt caggaacaat ccgaatatct aaaagtactt gcatatttgc acgagagaga
     6961 ttgctgatcg tggtgatgtt tcgattcggg atgaaatgga cagtaccatc cgttgctttc
     7021 agctgaaggg tacggatacc aacagatacg acggttcctt caatcgttaa gtttgctagc
     7081 ttgatgtagt ccccaacatc catctgttgt tccatgataa taaaaaatcc ggtaataaca
     7141 tcactcatga acccttgggc accaagaccg attgccacac ctgcaattcc ggcaccagcc
     7201 aaaagtgatc cgatcggcac accgatagca gataaaattg catagataaa aaagaaacca
     7261 agggtataat gaaaaatagt atttaaaaga ctatgtaaag tctttaagcg gctctcgttt
     7321 agaatggctt ttttagaata ctgattaaaa gacttatcaa tcagatattt tcccacacgg
     7381 tgaagaatcc aaaaaaggac gatcaaaaaa atcagatata aacttttttg gataaaggca
     7441 gccaaaatgg cgtcccaatc aatcccgtcc caaaaccgct gaaacgcatt tactttgctg
     7501 acagcttgat ctgtcaatgt ctcgccagaa gagtctgtcg taccggcagt tgctaataat
     7561 gaaaacataa aatcctcttt tctaaaattt gattctttct gtattgtacc aagaagtttt
     7621 caaaaaagga aatagcaaaa ataactataa tgaaggaaaa aagctgaaaa taaaatgaca
     7681 aataatatgg ataatgataa aatgtggaga aaataacgaa ggtggtaagt atatgcaaat
     7741 cgattggaac aatctaggtt tttcttatat caaaactcct tggcgtttta ttgccaaatg
     7801 gaaagacgga caatgggaag aaggagaatt gacagaagat aattacctga cgattcatga
     7861 aggctctcca gcgcttcatt atggtcagca atgttttgaa ggtctaaaag cttatcgtcg
     7921 aaaagacggc aaggtcaatc tctttcgacc tgaacaaaat agtcgacgga tgaatcaaag
     7981 tgctagacga ttgctgatgc caatggtccc tgaaaagatg ttcgttgatg ctgtcaagca
     8041 agtggtaaaa gcaaatgaag attttgtgcc gccctatgga acaggcgcta cattatactt
     8101 gcgcccttta ctgattgggg tcggtgaaaa cattggtgtc catccagctc cagaatattt
     8161 atttgtcgtc ttctgtacgc cagtaggagc gtatttcaaa ggtggactaa aaccaacgaa
     8221 ttttattgta tctgattacg atcgtgctgc tcctaagggg actggtgcag caaaagtcgg
     8281 cggaaattac gcagcgagtt tacttccagg aacagaagca aaagaactgg attactcaga
     8341 ttgtgtttat cttgatccag ccacacatac gaaaatcgaa gaagtaggtg cagcaaattt
     8401 ctttggaatt acaaaagacg gaaaattcat cactccgcaa tcgccctcta tcttaccaag
     8461 tatcacgaaa tattccttgt tgcagttagc tgaagaacga ctaggactgc aagcaattga
     8521 aggagatatt ttcattgatg atctagatca atttgcagaa gctggtgcat gtggtacagc
     8581 ggctgttatt tctccaatcg gtgggattta tcacaataac aatcttcatg tattttatag
     8641 tgaaacggaa gtaggaccag taacaaaacg gttgtatgat gaattgacag gtattcaatt
     8701 tggagacata gaagcaccag aaggctggat tgaagtcgtg gaataatttc aaagtaatga
     8761 gttgaaaaaa acgtaaaaag aaaaaatcag gaagagactt taaaaagttt tttcctgatt
     8821 tttatatgtt ttactttaaa gaatgcaaca aaagtttatg aaagatggac acctttgtca
     8881 acgtagataa tatcaccgat gattccgctg gaaagtgggc tgattaagaa agcacaagca
     8941 ttaccgactt cttcgatcgt cacaccttgc tgatcaggcg tacgagattc agataactgg
     9001 atcagttttt gatagtcttt aacacctgtg acagctaatg ttttgattgc cccagctgag
     9061 attccattga cccgaattcc tttaggcgag aactcagctg ccaaataacg gatagcagct
     9121 tcaagagaag ctttcgcaat acccatcatg ttataattcg ggattgcgcg ttctgaacct
     9181 agataagtca tgctaacgat cccgctgttt ggtgcaagaa acggttgtgc gtattttgat
     9241 acagcaatta aagagtagct gctgatatct tgagctaatt gatagccgtc tctggaaata
     9301 tcagacacat ttccggacaa ttcttctttg ttggcgtaag caactgcatg gacaaggccg
     9361 tggatctccc cagcatattc accgatggca ttcattgctt tttcgatgct ttcgtcgcta
     9421 gaaacatcac attctacagt gaatgcttct tcgccgacca atttgactaa tgattttttc
     9481 attcgttcat tttggtaagt atagataatt tctgcccctt gtttcaacat tgcttcagca
     9541 caaccccaag caatgctttt tttgttagcg acgcccataa tgacgatttt ttttccttct
     9601 agaaatgaca taatgactct cctttgtttc tgataaaaat ttgatagtcg taaaaaaata
     9661 gagaataata gctaaaataa cttcttgatt tcttaattat tagtcaaaat gttttgattg
     9721 tcaaactatt ttttcttaaa ttaaaaaaag attgctaaat atttaggaat atgatacttt
     9781 gatagtcaaa gtaatgaaaa tggcaattgc ctgaaagaag gagtttaatt atgtcttacg
     9841 tattatctgc tactgaagta atggaattga ttcctaatcg ttatcctatt tgttatattg
     9901 attatgtaga tactttagaa ccaggaaaaa agatcgttgc gacaaaaaat gtaacaatca
     9961 atgaagattt tttccaaggt cactttcctg acaatccagt gatgccgggt gcgttgattc
    10021 tagaaacact ggcacaagca ggttcgatac ttatcctaaa atcagaagag tttctaggaa
    10081 aaagagctta tatcggtggg atcaataagg ccaaattccg tcaaaaagtt gttccaggag
    10141 atgtgctgaa atgtcatttt gaaatcacca aaatgaaggg atcagtcggt acagcgttat
    10201 ctgcagcata tgtagatgat aaaaaagtct gtgagtgcga gttcacgttc atcgtcaacc
    10261 aaaatgcttg actataaaac ctgatatagc aaaacaaaag gaagcttact tgctttttat
    10321 gtaaaaagaa gacgcctgaa tcctagcata cgttgtcatc tatgcaggat tcagacgtta
    10381 tttttttatt tttgttttgc ttcttgcagt atattgattt tatcgatttt tacttcttct
    10441 tttggtttgt ctgaactatc tgtttcgata tttgcaatag catccacgac atccattcct
    10501 tcaatcactt ggccgaagac agtataatgg ccatctagtg aaggataacc gccgttttta
    10561 taagcgtcga tgattgcttg aggatagtcg tcttttaaaa gaccatcatg catatcatct
    10621 gtattttgaa cgataaagaa ctggctgccg ttactgttag gatcttgtga gcgagccata
    10681 gataacgcac ctcttatatt ataaaggtgg ttgttgatct ctgtttcaaa tccttctccc
    10741 caaatgcttt cccctccagt gccatcgcct ttaggatcgc caccttggat catgaaatct
    10801 ttgatgacgc gatggaaagt cagtccgttg tagtaaccgt cttttgcatg tgtcataaag
    10861 ttttcaaccg cttttggtgc gtgttcggga aataatttga ttttgattgt gcctttattc
    10921 gtcaccattt cgaccagatc ttcattttcg gcgacatctt tcgtcaattg tggtaattcc
    10981 aatgcgttca aatcgacgct actggcagtc gtgctttccg cagtagatgt actttttgaa
    11041 ctttctgtag acgaactaga agaagtcgtg ttgtttgcag aacaaccagc agcaaacagc
    11101 aatgctgctg ctaaactaat acttaaaata actttatttt tcttcataac tgttcctttc
    11161 caatttacta ttttgttatc tttaatgata acaaaataga gggaaaatac ctacttaaat
    11221 tattggaatt tatcatttat tttaaaagaa aaagaaaagg tgtagaccat ttactttgtg
    11281 tttgctataa tgtaagtgga tacaaaaacg aaaagatgca ggaggaaatg gtaatatggg
    11341 aaaattagga atctcgattt atccagaacg gtcaacattt gaaaaggaca aagcgtactt
    11401 ggatttagct cataagtatg ggtataaacg ggtttttacc agtttgctgc agatcaatga
    11461 tgataaagaa aaagttcttt cagaatttaa agaagtagtt gattacgcga atagcctagg
    11521 catggaagta atggttgata tcaatccagc gttatttgaa cagttggaga tttcttacga
    11581 tgacttatct tttttccata agatgggtgc ctacggggtc cgcttagata tcggattcac
    11641 aggagcagaa gaagcaaaaa tgacgcgcaa tccttttggt atcaaaatcg agatcaatat
    11701 gagttcgggc acgaattacg ttgacaacat catgagttat tcgccaaaca cggataattt
    11761 attaggttct cataattttt atcctcatcg atactctggt ttaggatacg aacactttgt
    11821 tttttgttca gaaaaattca gaaaatataa tttgaatacg atggcattcg tcaattcgca
    11881 atctgctgag tttggccctt ggcctacgca agatggcttg tgcacgctgg aagaccaccg
    11941 agatcttgaa attgcgacac aagtgaagca tttaatcctt acaggattga ttgatgatat
    12001 ttctattgga aatgcgtacg caagtgaaga agaattgaaa gaaatggcag aagcatttaa
    12061 cgcggattat ccaacattga aagtagatac ggaagaaggt attacagaaa acgaacgaat
    12121 ctgcttgttt gataaccttc atagttatcg aggggatcgc tcagaatata tccttcgttc
    12181 aacgatgact cgtgtatact acaaagataa agatttcccg cctcataata cgcgagatat
    12241 gcatcatggg gatgtactga ttgacaatga agggtacggt caatacaaag gagaaacaca
    12301 aatcgccttg aaggatatga aaaacgacgg acgtgtcaat gttgtaggaa gaatctccga
    12361 tgacgaatta ttcctccttg actttttgaa accatggtca agctttaaat taattgaaaa
    12421 taaaaaatag ataattagtt attgcagaaa taatccgcta gaaatagcca gttatttctg
    12481 cttttttatt ttgttttttt ataggttatg atttgtcaaa ttaagctaga ataaaatgct
    12541 tcttgtacat agctcaaaaa tatttcgatt ggtgttctgt aattcaatga ttttcttgga
    12601 atatgattac gttgattgct gacactggaa ataaatgtct gattcacttt tctaaaatcc
    12661 attgatttcg gcagtccatt acgacgcaga atcccgttag aattctcgtt taatgggcgt
    12721 tgagaaggtg ttccagggtc cgcaaaatat atatcaatat catgttggtt actaatggct
    12781 ttccagttag aaaattcttt tccacagtca aacgtaatag atttaaagaa gtttttaggg
    12841 aagcgagaaa accattgatt aagggcagtt tcaatatcta atgccttacg gccgttgggt
    12901 ttaatcgtga taatgacttt agataatctt tcaactaaag taatgacggc acttttatga
    12961 tgaatgccaa cgatcgtatc accttcaagg tgaccaaact cagaattgaa atcaggataa
    13021 ttatcaggtc gatcatggat tgagcgctga tactgttgtt ttccccgttt ttcctgatgg
    13081 ccattgggtt ttcttttacc tttcatcggt agtgtgtcaa tatcaaatat tcctttagaa
    13141 aataaacgat aaagtg
//
LOCUS       DAHEYX010000063        13071 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHEYX010000063 DAHEYX010000000
VERSION     DAHEYX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.064"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(88..312)
                     /locus_tag="MB219_001257"
     CDS             complement(88..312)
                     /locus_tag="MB219_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCC8426983.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(510..2090)
                     /locus_tag="MB219_001258"
     CDS             complement(510..2090)
                     /locus_tag="MB219_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCC8426984.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2491..3867)
                     /locus_tag="MB219_001259"
     CDS             complement(2491..3867)
                     /locus_tag="MB219_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCC8426985.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3995..5113)
                     /locus_tag="MB219_001260"
     CDS             complement(3995..5113)
                     /locus_tag="MB219_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCC8426986.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5255..5535
                     /locus_tag="MB219_001261"
                     /pseudo
     CDS             5255..5535
                     /locus_tag="MB219_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5613..6146)
                     /locus_tag="MB219_001262"
     CDS             complement(5613..6146)
                     /locus_tag="MB219_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCC8426987.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6240..7418)
                     /gene="mutY"
                     /locus_tag="MB219_001263"
     CDS             complement(6240..7418)
                     /gene="mutY"
                     /locus_tag="MB219_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCC8426988.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7411..8208)
                     /gene="recX"
                     /locus_tag="MB219_001264"
     CDS             complement(7411..8208)
                     /gene="recX"
                     /locus_tag="MB219_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCC8426989.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8315..9697
                     /gene="rlmD"
                     /locus_tag="MB219_001265"
     CDS             8315..9697
                     /gene="rlmD"
                     /locus_tag="MB219_001265"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCC8426990.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9871..10449)
                     /locus_tag="MB219_001266"
     CDS             complement(9871..10449)
                     /locus_tag="MB219_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCC8426991.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10771..11241
                     /locus_tag="MB219_001267"
     CDS             10771..11241
                     /locus_tag="MB219_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCC8426992.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11349..12575)
                     /locus_tag="MB219_001268"
     CDS             complement(11349..12575)
                     /locus_tag="MB219_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCC8426993.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3814 a         2654 c         2167 g         4436 t
ORIGIN      
        1 aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa
       61 cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac
      121 ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg
      181 gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttaaatctaa
      241 taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtatttgcca
      301 ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta
      361 tatttttttg attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa
      421 tccgtcacgg ctctaaacaa aatatgagag actgtttttg tttggctatt cttattactt
      481 gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattagga
      541 tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa tggttggtcg
      601 aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga
      661 tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca
      721 tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat
      781 tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt
      841 cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct
      901 ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc
      961 ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg
     1021 acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt
     1081 gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa
     1141 gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa
     1201 aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct
     1261 ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa
     1321 ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca
     1381 gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga
     1441 ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa
     1501 ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct
     1561 ttacccatac cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat
     1621 tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat
     1681 atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct
     1741 tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct
     1801 tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca
     1861 aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct
     1921 gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca
     1981 ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc
     2041 gcaaaagttc tgcgattgtc cacttgttgc tttaattctg gattattcat aacctaactc
     2101 catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag
     2161 aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa
     2221 acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc
     2281 tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca
     2341 actactacaa ctgaatcagt atagcatgga ttcatcgaat gaaaaagcca ccaaaccata
     2401 agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa
     2461 gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc
     2521 ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa
     2581 aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt
     2641 atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa
     2701 ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa
     2761 ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag
     2821 attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc
     2881 tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg
     2941 cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg
     3001 ctcctgcatg attttcttga tatctaaatt atcaaaaccg aatttatggg cagcttttag
     3061 taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat
     3121 tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc
     3181 aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga
     3241 aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat
     3301 ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat
     3361 acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc
     3421 aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat
     3481 acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat
     3541 gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga
     3601 taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat
     3661 tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc
     3721 tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga
     3781 agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag
     3841 cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta
     3901 gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt
     3961 ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct
     4021 tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc
     4081 agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta
     4141 ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca attacattag
     4201 gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg
     4261 cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc
     4321 ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca
     4381 caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt
     4441 gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt
     4501 ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca
     4561 acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc
     4621 caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt
     4681 aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt
     4741 cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca
     4801 atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg
     4861 caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca
     4921 acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa
     4981 ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca
     5041 tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa
     5101 gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat attcgttaaa
     5161 aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt
     5221 ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac
     5281 aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca
     5341 gggtatgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct
     5401 gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa
     5461 aatgaaatta cagcggatgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
     5521 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
     5581 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
     5641 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
     5701 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcggata attcatcatt
     5761 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
     5821 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
     5881 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
     5941 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
     6001 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
     6061 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
     6121 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
     6181 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
     6241 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
     6301 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
     6361 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
     6421 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
     6481 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
     6541 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
     6601 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
     6661 gctccagaat gattttgaag agccgcactg atataataaa cgtcttttgg ctttgctttt
     6721 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
     6781 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
     6841 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
     6901 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
     6961 actcgcatca cgttgccgtc aaccgcgggt tctggaagtc caaatgcgat acttgcaatc
     7021 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
     7081 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
     7141 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
     7201 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
     7261 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
     7321 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
     7381 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
     7441 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
     7501 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
     7561 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcca tcgcttgttg
     7621 gatcacttct tgagaaaaac ctttcgtcat caatgtttgt tggatttttc tcaataattc
     7681 tttttggctt tttccgcgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
     7741 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
     7801 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
     7861 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
     7921 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
     7981 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
     8041 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
     8101 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
     8161 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
     8221 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
     8281 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
     8341 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
     8401 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
     8461 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
     8521 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
     8581 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
     8641 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
     8701 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
     8761 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
     8821 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
     8881 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
     8941 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
     9001 aaaaaacgca atgatctgtg agatcaatga acgactgccc gaagttgttt caatcatgca
     9061 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
     9121 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
     9181 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
     9241 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
     9301 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
     9361 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
     9421 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
     9481 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
     9541 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
     9601 taaaaagttc aagattgaat atttacagtc tgtcgacatg tttcctcaga ctgcacgttg
     9661 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
     9721 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
     9781 ccgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
     9841 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
     9901 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
     9961 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    10021 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    10081 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    10141 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    10201 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    10261 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    10321 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    10381 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    10441 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    10501 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    10561 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga accaaatcat
    10621 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    10681 tcatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    10741 aaactactat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    10801 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    10861 ggagaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    10921 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    10981 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    11041 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    11101 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctaca tcatcgcctg
    11161 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    11221 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    11281 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    11341 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    11401 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    11461 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    11521 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    11581 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    11641 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    11701 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    11761 aactggtctt gtttgattgt ttcaaacgca ggggtgtagt tcgtcgctac atcaagcatg
    11821 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    11881 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    11941 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    12001 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    12061 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    12121 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    12181 gccatttcga caccaccaaa tgcataggca tgatttaatt tatctaccgt tccataacct
    12241 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    12301 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    12361 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    12421 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    12481 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac aaatacccct
    12541 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    12601 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    12661 ttaaaaaaca tttttgtgta acgattacta ttttttaaaa tttctcaaaa ttcttaacta
    12721 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    12781 ctttttttac gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    12841 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    12901 ttatcattcg atttcacgaa aatttcttcc tatgactggt ccttttgtta aacccatctt
    12961 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgatttc
    13021 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg a
//
LOCUS       DAHEYX010000064        13063 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHEYX010000064 DAHEYX010000000
VERSION     DAHEYX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.065"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..388)
                     /locus_tag="MB219_001269"
     CDS             complement(<1..388)
                     /locus_tag="MB219_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426994.1"
                     /translation="MKFIKFGIIGLLVVTVMGGCGTSTAETKESKNYSETNSTIKEVD
                     SDQTAEFATIAEKHIKEIYSIDNLKMDLKSIKVTQFPDDKNAETGEEYKNVYNGTGDF
                     TWQDKKYNFSLIYSKKDATNYTVLYLY"
     gene            complement(474..674)
                     /locus_tag="MB219_001270"
     CDS             complement(474..674)
                     /locus_tag="MB219_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426995.1"
                     /translation="MKIGMRKPSIKKSIGARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(695..1534)
                     /locus_tag="MB219_001271"
     CDS             complement(695..1534)
                     /locus_tag="MB219_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8426996.1"
                     /translation="MPRKPLSEEDKKARSIIANNLKKITHGYTRAQLSDMTGIPVTTL
                     SGYFTEKSTISMENAELIAKAFNVDKSDIDPRYSREYNYYDLKKEVLDELQENNKSFV
                     ADKFVELAEKANAAGMTLNRTKTDRGNIMTVLSSDIQEYNFFDTSVAAGIPSSVEAFD
                     QDHIEQIAIPDYAMGKYAGYQDVFFTKINGDSMNNVIPDGSLIAVKKIESCDELKDGD
                     IVVFSNDNEFSVKRYINDQQNNRIIFRPDSSNISFTDIVVNYDDTKYVQIYGKVVVYI
                     VQL"
     gene            1699..1947
                     /locus_tag="MB219_001272"
     CDS             1699..1947
                     /locus_tag="MB219_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8426997.1"
                     /translation="MELQITLEAARVMMGYSLKEAAKLFDVHHQTLANWEQDPNKMKQ
                     KYVQLIPEIYHFPTANIFFGSKDEFIRYKLHNDSFLIK"
     gene            1990..2745
                     /locus_tag="MB219_001273"
     CDS             1990..2745
                     /locus_tag="MB219_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCC8426998.1"
                     /translation="MKELIKVTTNEENEQLVNGRELHEFLEVATEYKKWFSRMAEYGF
                     VENIDFVRVTQKCPTPGGIQNITDHAMKLDMAKEISMIQRTEKGKQARQYFIEVEKEY
                     KQQLLDTSKLSPELQMFQGIFNAVAKQELETKRLATQMENITEIVALNTTDWRRECRK
                     LVNKMAETQGGYGAYQEIQTAIYEEVDRRAGSSLKTRLTNLRNRMAGEGVPKSKRDKT
                     NKLDVIESDKRLKEIYLSVVKDFAIKYGVWKEK"
     gene            2766..3032
                     /locus_tag="MB219_001274"
     CDS             2766..3032
                     /locus_tag="MB219_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8426999.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALNQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLVDHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            3046..3318
                     /locus_tag="MB219_001275"
     CDS             3046..3318
                     /locus_tag="MB219_001275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427000.1"
                     /translation="MTEITITDGHMSHTVKTKEEAQRIIDRYFPEGESTMQIRKPIVE
                     RSSSKMLLASIAEDKEPVYKVRQLQALCYISLVLNVILLSVVFAVL"
     gene            3337..4005
                     /locus_tag="MB219_001276"
     CDS             3337..4005
                     /locus_tag="MB219_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427001.1"
                     /translation="MSYNKWTREEEQLLIQNVRYDHRGFVCNCNELAELLGIDKKRIW
                     PKIHQMCKKDLIDEVYWDDPIEPPNKRYSQQEDKKIISMYQSGCPVTIIAQELNRTEN
                     AIRCRLNIMKKNGQLESNRKRRYTKREVELLISEIKFDKNGYVLNIDYLTRLLHRPKH
                     ELFRKICLLRKQGEITIKPDRTKASQNWYDVMKKQIDNHHKLIAARYSYQKKTRSPAS
                     KIVS"
     gene            4037..4366
                     /locus_tag="MB219_001277"
     CDS             4037..4366
                     /locus_tag="MB219_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427002.1"
                     /translation="MNEKIQNLIKELVAECANDDLGMSVSVVDEKGEVVLGQAGNDSL
                     VAWSVYRQYEKTKRDLQNNNCNCETHSTLKQLFGIEYEDPEFEEWYQDFLRFAEKMDR
                     KKGGIKF"
     gene            4366..4590
                     /locus_tag="MB219_001278"
     CDS             4366..4590
                     /locus_tag="MB219_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427003.1"
                     /translation="MISVKGLGDEMFEAMMHKAQQDVQDKILTAAKYGQTSCTVRSKG
                     LTPSFLAALESEGISNIQREEGSVKLFWEF"
     gene            4601..4951
                     /locus_tag="MB219_001279"
     CDS             4601..4951
                     /locus_tag="MB219_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427004.1"
                     /translation="MSVKDVAKKQILKMEHIIDQINEAEDLLISLKSPALNNLNELTT
                     DISIGVPTQFLGRGFFQEEQSRWHKVQLERDLGITEIQEEIRNLVSKSVQKRIEALES
                     ELRAMIYYRGDHDE"
     gene            4944..5138
                     /locus_tag="MB219_001280"
     CDS             4944..5138
                     /locus_tag="MB219_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427005.1"
                     /translation="MNDFDSLGARQQPPNEASPVGVDWQGNPLYPGDTCYLTEEGYVP
                     VDDILEYAQQHYPKIELGGI"
     gene            5141..5740
                     /locus_tag="MB219_001281"
     CDS             5141..5740
                     /locus_tag="MB219_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427006.1"
                     /translation="MDKSIRTDSDIVNHIKRLNLDGFSAGRSLVGNPATIADEKTIGL
                     FKEIKKVVKENGASYDQTRKALIMVDESFYQNLLKKENPFKEKQAEPINEPKKEDINV
                     TREDLESAELTIDPDLPGKLEKELRELSKEFFNKILASKKELLPSMKEEELVFLHKKL
                     NEFRRETPSDGFDANFHIGQILTDVILIVFAGDDLKYAD"
     gene            complement(5724..6074)
                     /locus_tag="MB219_001282"
     CDS             complement(5724..6074)
                     /locus_tag="MB219_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427007.1"
                     /translation="MYKLKKDNYNELSGICRSVMQNIKDLEEGSKMDLFDPRISDEEY
                     YLYSSVLKSYNSLGNQLLSNWETLKFYDIDAFFTKLSTYVEYLDIFIKEPTIENQLRL
                     TSIYDSLDKLNQRI"
     gene            6128..7150
                     /locus_tag="MB219_001283"
     CDS             6128..7150
                     /locus_tag="MB219_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCC8427008.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLANGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQDEVTRVADVPGHSEIEQAHLIENEDTDPIQEELLDIPDFGREEGVDDVSEFE
                     DDEYPF"
     gene            7113..7928
                     /locus_tag="MB219_001284"
     CDS             7113..7928
                     /locus_tag="MB219_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HCC8427009.1"
                     /translation="MSQNLKTMSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWSPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTAWKGKIDCLNVEEEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLYQQYGKSFMPIIAAVSKQTPSEARLITI
                     DQDNMDYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            7944..8723
                     /locus_tag="MB219_001285"
     CDS             7944..8723
                     /locus_tag="MB219_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8427010.1"
                     /translation="MAEGWVKLHRSITENWIWDNPQYLKWWLDLILMANHKEKKILFN
                     GSFKKVDVGQRITSEQKLAERWGVSRNTVRKFLSLLVEDDMISIEKSRKNGTTYKVNN
                     YAIYQGFSEEKKQQTEQRAEHQKDNELNINKNEKNEKNDKNNNNLRNSRKTREYADDD
                     PNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSNDFW
                     SGVILSPTSLRKHFDKMAIQKNKRTQQNISNDELPETGEDW"
     gene            8723..9571
                     /locus_tag="MB219_001286"
     CDS             8723..9571
                     /locus_tag="MB219_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCC8427011.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVSGSTIHAVITGRTGAGKTHLGAAVIKEVMMASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKTDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            9568..9810
                     /locus_tag="MB219_001287"
     CDS             9568..9810
                     /locus_tag="MB219_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427012.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            9953..10408
                     /locus_tag="MB219_001288"
     CDS             9953..10408
                     /locus_tag="MB219_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCC8427013.1"
                     /translation="MATRSKYGNKKHEVDGITFDSKAEARYYMKLKRNGMSFMPLSET
                     YCAMQENVLLQEGYLCNDRKIAPIYYRADFVIYENGQVKKVIDVKGYQDAISMLKMKM
                     FAHRYGFPVTFAKFDSKINKFIEMDCFESARQQRKRQTERRKKKLMEGK"
     gene            10410..10985
                     /locus_tag="MB219_001289"
     CDS             10410..10985
                     /locus_tag="MB219_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427014.1"
                     /translation="MTKQVNFRPELKKVTSKSNGNTEVLLVVSNGSLRGSTENLTEFL
                     GSTVTVVIQPETIEYTVPVNKQTKKPNVEYVVNADGTIDMLKEEQTSLDVGDGVEEVE
                     NVKILVSKETIDEFIKTATTLQLPENVTVNIRDVLIRLAEGDSMSEIAADHELSEIAL
                     IDQIELARHYFAPYADSWSKHKDDIIFPEEQ"
     gene            10982..11170
                     /locus_tag="MB219_001290"
     CDS             10982..11170
                     /locus_tag="MB219_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427015.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMDQ
                     KILDVGLLLLHLAEKNLI"
     gene            11178..11384
                     /locus_tag="MB219_001291"
     CDS             11178..11384
                     /locus_tag="MB219_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427016.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEASQWLLKTYTNNKKLRKKCPDL
                     FLKDDQIMPEPMILTSKEGGKCES"
     gene            11548..11946
                     /locus_tag="MB219_001292"
     CDS             11548..11946
                     /locus_tag="MB219_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCC8427017.1"
                     /translation="MIPRFRAYSKEENEMYYPHNDKNVDWTIDDETGFIAPLVNLGGG
                     MWGMIDKYELMQSTGLKDKNGVDVYQGDIIRCTRGCHHEVIWLEEYGGIFFGGMPAWY
                     LSGLSNGYAWTGGEEVIGNIYENSELLEEK"
     gene            11946..12245
                     /locus_tag="MB219_001293"
     CDS             11946..12245
                     /locus_tag="MB219_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427018.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVINNEI"
     gene            12235..12393
                     /locus_tag="MB219_001294"
     CDS             12235..12393
                     /locus_tag="MB219_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427019.1"
                     /translation="MKFKILEEDTRYELEEKLNEFAKNNEIQHISLTAFMAGYTAYYA
                     AVVSYVSQ"
     gene            12491..12907
                     /locus_tag="MB219_001295"
     CDS             12491..12907
                     /locus_tag="MB219_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCC8427020.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKYGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         4767 a         2027 c         2667 g         3602 t
ORIGIN      
        1 tatatagata taggacggta taattagttg catctttttt cgaataaatc aaagaaaagt
       61 tgtatttttt gtcttgccaa gtaaaatctc cagtaccatt gtagacattt ttgtactctt
      121 caccagtttc tgcattttta tcgtcaggaa attgagtaac tttgatagat tttaaatcca
      181 tcttcaaatt atctattgaa tatatttctt taatgtgttt ctcggcaata gttgcaaact
      241 cagcggtttg atcagagtct acttctttga tagtactgtt tgtctcacta taattttttg
      301 attcctttgt ttcagctgtt gatgttccac atcctcccat aactgtcact actagtaatc
      361 caataattcc aaatttaata aatttcattt tctactcctt atctctatga tattatttta
      421 ttggggaatc ttagaaataa ggtttcgagt ccgtgttgct gcacgggctt tttttattta
      481 aataagtccc aaaagctaaa cgtagtcttt ttataaactt tattgtatgc agcttttttt
      541 gggtctttaa tccatccaga gcctttcttt ccataaccag gaatcacagc ttttttcaca
      601 gctctttttg cctttccagt agttctagcg ccgatagatt tctttatgct cggttttctc
      661 attccaattt tcattttata ccctctcaaa ttaattatag ttggacaata tatacaacga
      721 ctttcccata aatttgaacg tatttagtat catcataatt aaccacaata tcagtgaaag
      781 aaatgttaga agaatcaggt ctaaaaatga tgcgattatt ttgttgatca tttatatatc
      841 tttttacaga aaattcgtta tcgttgctaa agactactat atctccatct tttaactcat
      901 cacagctctc tattttcttt acagcaatta atgatccatc aggaataacg ttattcatgg
      961 aatctccatt tattttagta aaaaatacat cttgatatcc tgcatatttt cccatggcat
     1021 aatcaggaat agcaatttgc tcaatatggt cttgatcaaa agcttcgact gaagaaggta
     1081 tgccggcagc aacagaagtg tcaaaaaagt tatattcttg aatatcactt gatagaactg
     1141 tcataatgtt tcctctatca gttttagttc tgttcaaagt catccctgct gcatttgctt
     1201 tttctgctaa ctctacaaat ttatcagcaa cgaaactttt attgttttcc tgtaattcat
     1261 ctaaaacttc tttctttaaa tcataataat tgtattctct ggaatatctt ggatctatat
     1321 cagatttatc aacattaaaa gctttagcta ttaattcagc gttttccata ctgatagttg
     1381 atttttcagt aaaatatccc gatagcgtcg taacaggaat acctgtcata tctgacaatt
     1441 gtgctcttgt gtagccatgc gtaatttttt ttaaattgtt agcgataatg gagcgagctt
     1501 ttttatcttc ttctgataat ggttttcttg gcatgttatt ttctcctcct tttttattaa
     1561 taatatcgaa ttaaatcaat ataaacaagt ataaagtagt tttataacag tgttttttaa
     1621 tttaaaaaac gtcaaaaatc gttgactggt agtataaact agtataaaat aatttacata
     1681 aacgaaggag gaataaaaat ggaattacaa attactttgg aagcagctag agttatgatg
     1741 ggatattctt taaaagaagc agcaaaactc tttgatgttc accatcaaac cttagcaaat
     1801 tgggaacaag atccgaataa aatgaagcaa aaatatgtgc aattaatacc agaaatatac
     1861 cattttccaa cagcaaatat tttttttgga agcaaagacg agtttatacg atataaactt
     1921 cataacgatt catttttaat aaaataactt atggaaaaca tatttattca tttttaagga
     1981 gtgtaatcca tgaaagaact aatcaaagta acaacaaatg aggagaatga gcagttagta
     2041 aacggcagag aattgcatga gtttttggag gtggcaacag aatacaaaaa atggtttagt
     2101 cgtatggcgg aatatggttt tgttgaaaat atagatttcg taagggtgac ccaaaaatgt
     2161 ccgacccctg gaggaattca gaatattacc gaccatgcaa tgaaattgga catggcaaaa
     2221 gaaatctcaa tgattcaacg aacagaaaaa ggaaaacaag cgcgtcaata ttttattgaa
     2281 gtagaaaaag aatacaaaca acaattactt gatacttcaa agctaagtcc agaacttcaa
     2341 atgttccaag gaatatttaa cgcagtagct aagcaagagt tagagacaaa acgtctagca
     2401 acacaaatgg agaatattac tgaaattgtg gcattgaata caactgactg gcgtagagaa
     2461 tgtcgaaagc tcgtaaataa aatggcggaa actcaaggcg gatacggtgc ttatcaagaa
     2521 attcaaacag cgatatatga agaagttgat cgtagagctg gctcctctct caaaactaga
     2581 ttaaccaatt tacgtaaccg tatggctggc gaaggtgttc caaaatccaa gcgagataag
     2641 acaaataagt tagatgtaat cgaaagcgat aaaaggttaa aagaaattta tctatctgta
     2701 gtcaaggatt tcgctattaa gtatggagtt tggaaagaaa aataaaattc gataggaggc
     2761 aagtcatgaa tattctaagc gaagagtttc tgactcgttt gagaattgca attgttgaag
     2821 ttgtgaagga cgcacttaat cagctttcaa aaaagaatct ttcagaaaca cgatatttga
     2881 aaaaaatcga agctagaaag tatgtgggag gtgtaaatga tcaaggcttt gagaagctag
     2941 tagatcatgg gttaaaagaa attcgtatag atggcttttt aaggtatgac aaaaaagaca
     3001 tcgatgaact gatggctaaa tacaaaatct aaaaggagga catacatgac agagatcacc
     3061 atcacagacg gacatatgtc tcataccgtg aaaacaaaag aagaggcaca acgtattatt
     3121 gatcgatatt ttccggaagg ggagtcaact atgcaaataa gaaaaccaat cgttgagcgt
     3181 tcgagtagca aaatgctact ggcatcaatt gccgaagaca aagaacccgt gtacaaagtt
     3241 cgtcagttac aagcactttg ctatatcagc ttagttctaa acgtcatttt attgtctgtt
     3301 gtctttgccg ttttataaaa aaaggaggtt agaaaattgt cgtacaacaa atggacaaga
     3361 gaagaggagc aacttcttat tcaaaatgtc agatacgacc atcgcggatt tgtttgtaat
     3421 tgtaatgaat tagctgagtt acttgggata gataaaaagc gtatttggcc caaaatccat
     3481 caaatgtgta aaaaagattt gatcgatgaa gtttattggg atgatccaat cgaaccgcct
     3541 aacaaacgtt acagtcagca agaagataaa aaaataattt ctatgtatca gtcaggttgt
     3601 ccagtaacaa taatagcgca ggaattgaat agaactgaga acgcgattcg ctgtcgtctt
     3661 aatatcatga agaaaaatgg acaattagaa agcaatcgaa aacgcaggta cacaaaaaga
     3721 gaagttgagt tattaatatc agaaattaaa ttcgataaaa atggttacgt attaaatatt
     3781 gattatttaa ctagactact gcatagacca aagcacgagc ttttcagaaa aatttgtcta
     3841 ttaaggaaac aaggagaaat aactataaaa cccgatcgta caaaggctag tcagaactgg
     3901 tatgacgtaa tgaaaaaaca aattgataat catcacaaac tgatagctgc tcgatatagc
     3961 tatcaaaaaa agacacgatc gccggcaagc aaaatcgtgt cctgaaaaat aataattaca
     4021 aggagagttt atcacaatga atgaaaaaat ccaaaattta attaaagagt tagtagctga
     4081 atgtgcaaat gatgatctag gaatgtcagt tagtgtagtc gatgaaaaag gggaagtcgt
     4141 tcttggacaa gctggaaacg atagcttggt tgcgtggagt gtttaccgac agtatgagaa
     4201 aacaaaacgt gatttgcaga ataataattg caattgtgaa actcatagta ctttaaaaca
     4261 attgtttggt attgaatatg aagatccaga gtttgaggaa tggtatcagg actttctacg
     4321 gtttgccgaa aaaatggatc gcaagaaagg tggtatcaaa ttctgatgat ctcagttaaa
     4381 gggctaggag atgaaatgtt cgaagcaatg atgcacaaag cacaacaaga tgtacaagac
     4441 aaaatcttaa ctgcagcaaa gtatggacaa acaagttgca ctgttcgttc aaaaggtctg
     4501 acaccatcat ttctagcagc attagaaagc gaaggtattt caaacatcca acgtgaagaa
     4561 ggaagtgtca aattattttg ggaattttag gaggcaaatg atgagtgtaa aggatgtagc
     4621 taaaaagcaa attttaaaaa tggaacatat cattgatcaa atcaacgagg cggaagattt
     4681 acttatcagc ttgaaatcac cagcactgaa taatcttaat gagcttacga ctgatatttc
     4741 catcggtgtg cctactcaat ttttagggag aggttttttc caagaagaac aaagcaggtg
     4801 gcataaggtc caacttgaac gtgatcttgg tatcacagag attcaagaag aaattcgtaa
     4861 tcttgtttcc aaatcagttc aaaaaaggat tgaagcactt gaaagcgagt tgagagcaat
     4921 gatttattat cgcggtgatc acgatgaatg attttgattc tttaggcgcc agacagcaac
     4981 caccaaatga agctagtcct gtcggagttg attggcaggg aaatccgttg tatccaggtg
     5041 atacttgcta tctaacagaa gaaggctatg taccagtaga cgacattcta gagtatgcgc
     5101 aacaacatta tccaaaaatt gaattaggag gaatttaggg atggataaaa gtatacgtac
     5161 tgattcagat attgtgaatc atattaaacg gttaaactta gatggcttta gcgctggccg
     5221 ttctcttgtt gggaatccag ctactatcgc tgatgaaaaa acaataggtc tatttaagga
     5281 aataaaaaag gttgtaaaag aaaatggggc aagttatgac caaacgagaa aggccctcat
     5341 tatggtagat gaaagttttt atcaaaactt attgaaaaaa gagaatccat ttaaagaaaa
     5401 acaagctgaa ccaattaatg aacctaaaaa ggaagatatc aacgtaacaa gggaagattt
     5461 agaaagtgcc gagttaacaa ttgatcctga tttacctgga aaactagaaa aagaattgcg
     5521 ggaactaagc aaagagtttt tcaataaaat tctggcatca aaaaaagaac ttctcccctc
     5581 aatgaaagaa gaagaactgg tttttttaca caaaaaatta aatgaattta gaagagaaac
     5641 tccatccgat ggattcgatg caaattttca tatcggacaa attctgacag atgtgattct
     5701 gattgttttt gcaggtgatg atcttaaata cgctgattaa gcttatcgag actgtcgtaa
     5761 attgacgtta atctcaactg attttctata gttggttctt tgatgaatat atccaaatat
     5821 tcgacatacg tactcagttt agtgaaaaaa gcgtctatat cataaaattt taaggtttcc
     5881 caattactga gcaactgatt tcctaaagag ttatagcttt taagaacaga tgagtataaa
     5941 taatattctt catcactaat acgaggatcg aataagtcca ttttactacc ttcctctaaa
     6001 tctttaatgt tctgcattac tgagcgacat attccagaaa gctcattata gttgtctttt
     6061 ttcaatttat acatttttat cgcctcactt tcaaaataag tatagcaaaa ataaaggaga
     6121 ataaaatatg gcaaatgact taacacaaac aacacaacgt tcattagatg aacaggtaat
     6181 cagtaatctt ggacggttac aggaacaagg cttagaaatg ccaccaggat atagtccgca
     6241 aaacgcgttg aagagtgctt ttttcgaact cactaacaat acaggtggaa atttattaca
     6301 gatggctgca aacaatcaag aaatgaaaac ctccatttct aatgcacttt tagacatggt
     6361 tattcaaggg ttatctccag caaaaaaaca atgctatttc attaaatacg gaaacaaggt
     6421 tcagctaatg cgttcatatt ttggaacaat ggcggtcctt gatcgtgtaa ctggaggggc
     6481 agatattacg cctgtagtcg ttcgacaagg tgatgagttt gaagttgcaa tggatggacc
     6541 gaacatggtt gtcaaaaagc atgaaacaaa attcgagaat ttagacaatg aaatcattgc
     6601 agcctatgta gttatcaagt tagcgaatgg taaagagacc acaacagtca tgacgaaaaa
     6661 gcaaatcgat cagagttggg cgaagtcaaa aatgaaaggt tctggaccac aaaaggaatt
     6721 tccagaagaa atggctaaac ggacagtaat caatcgtgca gctaaaacat tgatcaacac
     6781 aagcaacgac aacgatttat tagttcaagc tgcaaaagat acgttagaaa atgaatttga
     6841 caatgatcgg aaagatgtaa cgccacaaac agaaaaagtg gctactctcg aacaaaaatt
     6901 cttttcaaac aaaaagatta ctgaaccaat acaaaaagaa ccagatccga ttgtgattcc
     6961 agatgatatc caagatgaag taactcgagt tgctgacgta cctggccatt cagaaataga
     7021 acaagcacat ttaatcgaaa atgaggatac cgatccgatt caagaagaat tattggatat
     7081 tccggacttt ggacgtgagg aaggtgtaga cgatgtctca gaatttgaag acgatgagta
     7141 ccctttctga tgaaaattat tactccaatg aagctgactg gcagtacatg tcgacatcac
     7201 aatataagtc ctttttaaaa tgtgaagccg cagccttagc aaagttgaaa ggcgattggt
     7261 caccgacgtc ggatcccaaa gccttactcg tcggaaacta cgtacattct tattttgaat
     7321 caaaagaagt acatgaagcg ttcaaagaag aaaataaatc caaaatgttt tctagtcgga
     7381 agccctatgg attgttaaaa gatttccaga ttgccgaaca aatgattgaa cgacttaagc
     7441 aagaagaagc attcatgaat atctatcaag gtgagaaaga aacgattgtg actggggaac
     7501 tttttggaac ggcgtggaaa ggcaaaattg attgtctgaa tgtagaggaa gagtattttg
     7561 ttgacatcaa aacaaccaaa gacatgcacg agcggaaatg gaatgaaaac tacggatcaa
     7621 gagaaacgtt cattgtcaat tttggttacg tacttcaaat ggcaatttac caggaactgc
     7681 tttaccaaca atacggaaaa agttttatgc cgatcatcgc tgcagtgtcc aagcagacac
     7741 caagtgaagc aagactgatc acgattgacc aggataatat ggattatgag ctggttatgt
     7801 taaaagaaaa aatcgaaaga attgtcagag tgaagaacgg agaagagaag ccgaaccatt
     7861 gcggtttatg cgagtactgt aggggtaatc ttccaataac tggattcact agcatggacg
     7921 atttatagaa cggtggtgat tatatggctg aaggatgggt caagcttcac cgttctataa
     7981 ctgaaaattg gatatgggac aatccgcaat atctaaagtg gtggcttgat ttaattctaa
     8041 tggccaacca caaagaaaag aaaattcttt ttaatggttc atttaaaaaa gttgatgttg
     8101 ggcaaagaat tacttcagaa caaaaattgg ctgaacgttg gggagtaagt agaaatactg
     8161 tcagaaaatt tttaagtcta cttgttgaag atgacatgat aagcattgaa aaaagcagga
     8221 aaaatggaac aacgtataaa gtcaataact acgccattta tcaagggttt tcggaagaaa
     8281 aaaaacaaca gactgaacaa cgagctgaac atcaaaagga caacgaactg aacataaaca
     8341 agaatgaaaa gaatgagaag aatgataaga ataataataa tcttcgcaac tctcgaaaaa
     8401 cacgagagta tgcagatgac gatccaaata aaaaattggc cattctttta ttaaaactca
     8461 ttcgaaaaaa tcaaaacatc aaagaacctg atttggataa atgggcgaat acgattcgtt
     8521 taacaattga atctgacaaa cgaactggta gagaagttca agacatgatt gtttgggcca
     8581 caagtaatga tttctggtca ggcgtaatct tatctccaac gagtttgaga aaacatttcg
     8641 ataaaatggc tatccaaaaa aataaaagaa cgcaacaaaa tatttctaat gatgagttac
     8701 cagaaacagg tgaggattgg taatgaataa aaaactaagt gcaatggctg caccttacgg
     8761 cggattaaaa acagcagatc ataattgccc gaaatgtggt gatccattgt atatctggaa
     8821 aacaaaaaat aaagatggta ctgatcgatg cggtcctaca tgtatcaata agatttgtgg
     8881 ttaccgagaa atggtaacta aaaatcaaaa agaagctatc caaaaagcga atgaagcaat
     8941 gaaaaaggac gctatcaatc gaatgcttaa cagttcaatg attacagatg atgccatatg
     9001 gaccttcgat tttgatggat acaaagtagt tgatcaggaa acagcacaaa taaaagcgat
     9061 ggctcaagaa tgggctaaaa aaatcgtaag tggcagcacg attcacgcgg ttattactgg
     9121 tagaacagga gccggaaaaa cccatctagg tgctgcagtg atcaaagaag taatgatggc
     9181 atctaattat aaaattgcct gttcatttat aagctatcga gaattattag agcaattgaa
     9241 atttgcaatg aatgatccag aagcaagaaa agctgtgaca ggttcgttga tggctgaaat
     9301 taaaaagacg gattttgtag tgattgatga tttaggtgcg gagctaggaa gaatggaaga
     9361 aaacaatcag gcaacgcctt atgatgttga tgttctcaca tcgctcacag aagctcgtct
     9421 aaacaaagct acgatattca caaccaattt atcatcgaag caattaaaac acgcatacgg
     9481 cgagcgagtg ttctctcgtg taatgaatgg gacaaaagga aacatagccg tattcaaaac
     9541 aacgacagac aaaaggagga atccagtttg acctttgtag tgaaaaaaat gtgctactta
     9601 gatagccgag ggcgaggaga agccagtgtc gagtttgcta aacaccacac gactaaagaa
     9661 gaggctgacc ttgttgcaag tgtttgtggc ggcgaagtag tcgaagttat taaacccaaa
     9721 agacgatttt caaaacctaa gacaaagcca gtgaaaaaag agtgttgttg tccgaaaagt
     9781 aaccaagcat ggatgagagg tgcaaaatga cttgtttaaa atgcaacgat gaaacagtta
     9841 tttggtataa gacatcgctt ggatggtcaa cttgtgaacc ttgtccaata tgcaatgaaa
     9901 atggacgacg ttcgaaagaa cgactcgaaa gactaaagaa ggagtatagc aaatggcaac
     9961 aagaagcaaa tacggaaaca aaaagcacga agtagacggc atcacttttg attctaaagc
    10021 agaagctcgt tattacatga agttaaaacg aaacggtatg agttttatgc ctttatctga
    10081 aacctactgt gccatgcaag aaaatgttct gctgcaagaa gggtatctat gcaacgatcg
    10141 taagattgca ccgatttatt accgagctga ttttgtgatt tatgagaatg gccaagtgaa
    10201 aaaagtgatt gatgtcaaag gttatcaaga tgcgatctct atgctgaaaa tgaaaatgtt
    10261 tgctcatcga tacggctttc cagtgacatt tgctaagttc gattcaaaaa tcaataaatt
    10321 cattgaaatg gattgttttg aatcagcaag acagcagcgg aaaagacaaa cggaacgaag
    10381 aaaaaagaaa ttaatggagg gaaaataaaa tgacaaaaca ggtgaatttc agaccagaat
    10441 taaagaaagt aacatcaaaa tcaaatggga acacagaagt attactagtt gttagcaacg
    10501 gatcattgag aggtagtact gaaaatttaa ctgaatttct tggctcaaca gtgactgtag
    10561 taatccagcc agaaactatc gaatacacag tgccagtaaa taaacaaacg aaaaaaccga
    10621 atgtcgaata cgttgtaaat gctgatggca caatcgacat gcttaaagaa gagcagactt
    10681 cacttgatgt aggtgatgga gtagaagaag ttgaaaatgt gaaaatccta gtatcaaagg
    10741 aaacgattga tgaatttatt aaaacagcaa cgacattgca actaccagaa aacgtaactg
    10801 taaatattcg agacgttctt atccgtttag cagaaggcga tagtatgagt gaaattgctg
    10861 cagatcatga attatcagag attgctttaa tagatcaaat cgaattagct agacattact
    10921 ttgctccata tgcagatagc tggtccaaac ataaggacga catcattttt ccagaggaac
    10981 aatgagagca actgatccag taattatcct tgaggaagct aaatttattt ggactcacga
    11041 agagatagag caagcacgct tgctcttttc tcaaggagtt aagccgagca aagtagctga
    11101 aataatggat caaaagattc ttgatgtcgg attgcttttg ttacatctag cagaaaaaaa
    11161 tttgatttga ggtgaaaatg atgattcaac ttgcaggcat acaaacaggg aaaatttatt
    11221 tttccggaga aagcaaaagt gaagctagtc aatggttgct taaaacatat acgaacaata
    11281 agaagctgag aaaaaaatgt cctgatctat ttttgaagga tgatcagatt atgccagaac
    11341 cgatgatttt gacaagcaag gagggcggca aatgcgaatc ttagagattg tttgggaagt
    11401 aattaaattt atttccaata tgtattttac gttttggtta gtgtgctgcc ttctatatgg
    11461 attcaaatgg caagtaggga atttcatgag cgttaaattg ccggggatat tgagacggag
    11521 taaaagaatt tacaaggagg acagcaaatg ataccgagat ttcgagcgta ttcaaaagaa
    11581 gaaaatgaaa tgtactatcc gcataatgat aaaaatgtgg attggacaat agacgatgaa
    11641 acaggcttta ttgctccact tgtaaattta ggcggcggca tgtggggaat gatagacaaa
    11701 tacgaactta tgcaatcaac aggactgaaa gataagaatg gtgtggatgt ctaccaaggc
    11761 gatatcatca gatgtactag aggttgtcat catgaagtaa tttggttaga ggaatatgga
    11821 ggaatatttt ttggaggaat gccggcgtgg tatctttcag gattaagtaa tggctatgcg
    11881 tggactggtg gagaagaagt catcggaaat atatacgaga atagcgagtt attggaggaa
    11941 aaataatgaa actaaaagac ggattttacg ctagtagcca cggtatcggc ggtttaatgc
    12001 tagacatgcc gacaaagaat cctaaaacac gtgagaaacc aaaagtcaaa gtcggtgata
    12061 tggttcgctg tgaagcagaa gggttcatct atccgtttcg tggatatgta gagcatctct
    12121 ataatcactc agcaatcatt cgtattgaaa acacgatgaa atgtgacaag tggttagcga
    12181 aaagtaaaga gaatttagca gtggtgagat tggtggatat ggaggttata aacaatgaaa
    12241 tttaaaatcc ttgaagagga cacacgctat gaattagaag aaaaattaaa tgaatttgca
    12301 aagaataatg aaattcagca tatatcttta acggctttta tggccggtta tacagcttac
    12361 tatgcagctg ttgtgagcta cgtaagtcaa taaagggaac tcgacaaata aaaaaagccg
    12421 gatcgctccg actcaaaata aataaacacg acacgtttat tatatcacat aaaaggagcg
    12481 gtttgacttg atgcaattgt tacgagaggt agatttcaaa cagacaagat gtaatgcgag
    12541 agatgtgctg aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat
    12601 taagtcgcct atcataaccg atatgccgaa ggctccgaaa tatggaaaca aggcagaaga
    12661 cgcgatcata cagatgatgg atatagaagc ggagagagac gcgattttag cggctttgat
    12721 ggctctcagt ctgattagtc gtcagatact ctactacagc ttctgtgacg taaataagca
    12781 ctctaattat gaaatagggc aattgatacg aggatacgga gagaagaatg tagagaagct
    12841 gaaatccatc gcattgatag aatttgcaga agcatacaaa aaaggcgtgt tagttcagta
    12901 tcgttgattt tgtagggttt ttgtagggat agtgtagggt ttttgagtgt tttaacgtga
    12961 tattatgata gtgtcgaaag attagtgata ggtctgagac aaaataataa taaaaggaac
    13021 atcgttttat tattgtttca caattaagct tcgatagaca gca
//
LOCUS       DAHEYX010000065        13050 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHEYX010000065 DAHEYX010000000
VERSION     DAHEYX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.066"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(80..152)
                     /locus_tag="MB219_001296"
     tRNA            complement(80..152)
                     /locus_tag="MB219_001296"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Lys,seq:ctt)
     gene            533..1102
                     /locus_tag="MB219_001297"
     CDS             533..1102
                     /locus_tag="MB219_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCC8427021.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1089..1949
                     /locus_tag="MB219_001298"
     CDS             1089..1949
                     /locus_tag="MB219_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCC8427022.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2139..2843
                     /locus_tag="MB219_001299"
     CDS             2139..2843
                     /locus_tag="MB219_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8427023.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2848..4683
                     /gene="walK"
                     /locus_tag="MB219_001300"
     CDS             2848..4683
                     /gene="walK"
                     /locus_tag="MB219_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCC8427024.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4680..5996
                     /locus_tag="MB219_001301"
     CDS             4680..5996
                     /locus_tag="MB219_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427025.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5997..6866
                     /locus_tag="MB219_001302"
     CDS             5997..6866
                     /locus_tag="MB219_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCC8427026.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6921..7730
                     /locus_tag="MB219_001303"
     CDS             6921..7730
                     /locus_tag="MB219_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCC8427027.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7833..8462)
                     /locus_tag="MB219_001304"
     CDS             complement(7833..8462)
                     /locus_tag="MB219_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCC8427028.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8632..9924
                     /locus_tag="MB219_001305"
     CDS             8632..9924
                     /locus_tag="MB219_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8427029.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10000..10662)
                     /locus_tag="MB219_001306"
     CDS             complement(10000..10662)
                     /locus_tag="MB219_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCC8427030.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10887..11171
                     /gene="groES"
                     /locus_tag="MB219_001307"
     CDS             10887..11171
                     /gene="groES"
                     /locus_tag="MB219_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCC8427031.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11222..12847
                     /gene="groL"
                     /locus_tag="MB219_001308"
     CDS             11222..12847
                     /gene="groL"
                     /locus_tag="MB219_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCC8427032.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4341 a         2254 c         2824 g         3631 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
      181 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
      241 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
      301 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
      361 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
      421 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
      481 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
      541 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
      601 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
      661 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
      721 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
      781 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
      841 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
      901 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
      961 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     1021 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     1081 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag
     1141 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     1201 aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg
     1261 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     1321 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat
     1381 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     1441 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     1501 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     1561 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     1621 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     1681 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     1741 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     1801 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     1861 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     1921 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     1981 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     2041 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     2101 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     2161 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg
     2221 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     2281 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     2341 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     2401 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     2461 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     2521 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     2581 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     2641 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     2701 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     2761 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     2821 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     2881 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     2941 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     3001 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     3061 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     3121 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     3181 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     3241 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     3301 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     3361 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     3421 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     3481 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     3541 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     3601 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     3661 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     3721 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     3781 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     3841 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     3901 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     3961 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     4021 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     4081 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     4141 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     4201 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     4261 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     4321 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     4381 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta
     4441 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     4501 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     4561 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     4621 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     4681 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     4741 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     4801 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     4861 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     4921 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     4981 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     5041 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     5101 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     5161 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     5221 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     5281 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt
     5341 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     5401 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     5461 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     5521 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     5581 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     5641 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     5701 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     5761 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     5821 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     5881 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     5941 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     6001 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     6061 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     6121 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     6181 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     6241 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     6301 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     6361 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     6421 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     6481 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     6541 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     6601 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     6661 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     6721 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     6781 ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     6841 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     6901 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     6961 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     7021 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     7081 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     7141 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     7201 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     7261 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     7321 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     7381 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     7441 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     7501 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     7561 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     7621 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     7681 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     7741 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     7801 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     7861 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     7921 atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     7981 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     8041 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     8101 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     8161 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     8221 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     8281 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     8341 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     8401 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     8461 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     8521 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     8581 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
     8641 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
     8701 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
     8761 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
     8821 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
     8881 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
     8941 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
     9001 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
     9061 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
     9121 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
     9181 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
     9241 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
     9301 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
     9361 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
     9421 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
     9481 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
     9541 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
     9601 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
     9661 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
     9721 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
     9781 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
     9841 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
     9901 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
     9961 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    10021 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    10081 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    10141 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    10201 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    10261 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    10321 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    10381 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    10441 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    10501 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    10561 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    10621 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    10681 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    10741 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    10801 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    10861 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    10921 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    10981 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    11041 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    11101 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    11161 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    11221 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    11281 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    11341 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    11401 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    11461 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    11521 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    11581 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    11641 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    11701 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    11761 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    11821 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    11881 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    11941 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    12001 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    12061 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    12121 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    12181 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    12241 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    12301 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    12361 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    12421 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    12481 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    12541 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    12601 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    12661 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    12721 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    12781 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    12841 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    12901 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    12961 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
    13021 acgtgtttag agcgtgagac aaaagtgttc
//
LOCUS       DAHEYX010000066        12794 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHEYX010000066 DAHEYX010000000
VERSION     DAHEYX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.067"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            245..1768
                     /locus_tag="MB219_001309"
     CDS             245..1768
                     /locus_tag="MB219_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCC8427033.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            2005..3345
                     /locus_tag="MB219_001310"
     CDS             2005..3345
                     /locus_tag="MB219_001310"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCC8427034.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            3498..4235
                     /locus_tag="MB219_001311"
     CDS             3498..4235
                     /locus_tag="MB219_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCC8427035.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(4400..4621)
                     /locus_tag="MB219_001312"
     CDS             complement(4400..4621)
                     /locus_tag="MB219_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCC8427036.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            4652..5683
                     /locus_tag="MB219_001313"
     CDS             4652..5683
                     /locus_tag="MB219_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427037.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            5670..7073
                     /locus_tag="MB219_001314"
     CDS             5670..7073
                     /locus_tag="MB219_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCC8427038.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            7139..7774
                     /locus_tag="MB219_001315"
     CDS             7139..7774
                     /locus_tag="MB219_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCC8427039.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            7823..8503
                     /locus_tag="MB219_001316"
     CDS             7823..8503
                     /locus_tag="MB219_001316"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCC8427040.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            8638..9867
                     /gene="rpsA"
                     /locus_tag="MB219_001317"
     CDS             8638..9867
                     /gene="rpsA"
                     /locus_tag="MB219_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCC8427041.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(10016..11356)
                     /locus_tag="MB219_001318"
     CDS             complement(10016..11356)
                     /locus_tag="MB219_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCC8427042.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(11632..11787)
                     /locus_tag="MB219_001319"
     CDS             complement(11632..11787)
                     /locus_tag="MB219_001319"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCC8427043.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(11971..12174)
                     /locus_tag="MB219_001320"
     CDS             complement(11971..12174)
                     /locus_tag="MB219_001320"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCC8427044.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(12084..12416)
                     /locus_tag="MB219_001321"
     CDS             complement(12084..12416)
                     /locus_tag="MB219_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8427045.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         4114 a         2199 c         2716 g         3765 t
ORIGIN      
        1 caaatgtgaa attcttctcc ttttaaaaga cgaaaatgtt tgtttataaa aaaaaggaaa
       61 cgctacaatg aaaaagaaga aagctttaaa aacctgaaaa aagaggaggg aatagggaaa
      121 aacgaacttt ttgaacaaaa gttttacaaa atgcgtgtta ttatttttgt tctgatactc
      181 tttttgataa aataacatta gagccaaaat tgttgtctct aacggaaaag aaaggaagaa
      241 gaagatggaa caaacaaaaa agaaaaaatt tcaaatgcct tcagcgtata cggtcttatt
      301 tattattatt gcagtaatcg ctgtacttac ttggtttata ccagctgggc actatagtgt
      361 agacgatgca ggcaacatcg tcgctggatc atatgaacga gtacaacaaa atcctcaagg
      421 gctgtgggat attttcatgg cgccaatcaa tgggatgcta ggtgtaccag cgggggagct
      481 aactaaagaa acacctgctg caatcccaat ctctttcttc attttggttg ttggtggatt
      541 tttaggtatt atcaacaaaa caggggcact agatgccggg attgcttcag ttgtgaagaa
      601 attcaaagga aaagaaaaaa tgcttattcc agtattgatg ctgttatttg cattaggcgg
      661 ctctacttac ggaatggcag aagaaacgat tccattctat gctttactga tcccagtcat
      721 gatggctgta ggctttgata cagtagtagc tgtagcaatc gtattagtag ggtctcaagt
      781 aggctgtcta gcttcaacgg taaatccttt tgcaacaggc gtagcttcac aagctgttgg
      841 gatttcgatg ggtgaaggta ttggtcttcg attcttgatg ttcgttattt tgttagctat
      901 ttctattgta tatgtgtatc gttatgcatc aaagatcgaa aaagatccaa caaaatcatt
      961 agtatacaac caacgtaaag aagatatgaa gcatttcgat attgaagcaa tcggtcgtca
     1021 agaagtaatc acgaaaaaac aaaaacatgt taatgtattg ttctttatta cttttggtat
     1081 catgatcatc agtttgattc cttggacaac cttgaatgcg aatttcacga tttttgaatc
     1141 attgacaaac tggttaacaa accttccggt actaggtacg gttctaggta aaagcatgct
     1201 tccattaggt gactggtact tcccagaaat tactatgtta ttcttagtaa tgtccatcgt
     1261 agtagcaatc acttatggca tgaaagagtc tgaatttgtc tctgaatttt tagctggtgc
     1321 ttctgatttg atcggtgtag caatcgtagt agctgttgct cgtggtatcc aagtggttat
     1381 gaacaatgga ttaatcacag acaccatctt acattggggc gaacaaggat taagttcatt
     1441 atcttcaagt gtattcatca ttattacatt tattttctat atccctatgt cattcttgat
     1501 tccttcaact tccggcttag ctgctgcaac aatgggaatc atgggaccta tgggtgaatt
     1561 tgccggcgtc ggtaaggatt tagtaatcac tgcttatcaa tctgcatctg gtttagtaaa
     1621 cttaattaca ccaacatctg ctatcgtaat gggtgctgta gcaattgctc gtttcgatgt
     1681 ttcagtgtgg tggaaattta ccggtaaatt aattgcgata ttatttgtag ccatctgtgt
     1741 gatcctaggt gtagcagcaa tgttctaaac ctagacaaat acaggtacaa ggtctatcat
     1801 aagaatgaag aaaccgataa acggctatcg ataagcacgt ggataagtca atagctccat
     1861 tcagtgagtg ttttttcttt catcatttgt taagaacatt ctgcttgctg atggagcagg
     1921 cttaaaacgt cattatttga gaagaagagg ttgtgtcgga ggaatttgac atgacccttt
     1981 ttaacatatt aggaggataa tatcatgaaa aaatttgtga cagaacaaca tcatgaacaa
     2041 gcattagaat cattgagcga attgatccga attccttccg tactagatga agcagattca
     2101 ggacaaggac atcccttcgg taaaaaagtg attgaagctt tggataaagt attggaaatc
     2161 agtgaaaagc taggatttag aacatttaaa gatccagaag gctattacgg ttattcagag
     2221 attggctctg gagatgaatt attcggtatc ttgtgtcata tggacgttgt tccagcaggg
     2281 gacgaaaata attgggaaac aaaaccattc gatccaacta taaaagacgg ctggttagtt
     2341 ggtcgcggat cccaagatga caaaggtcct tctattgcgg caatgtatgc cgtcaaagct
     2401 ttgattgatg ctggcgtgga attcaagaca cgtattcgtt ttatctttgg tacagatgaa
     2461 gaaaatttat ggcgttgttt agaaaaatat aacgaaaaag aagaaggaat tacgcaagga
     2521 tttgcgccag atgctgaatt tcctttaatt tatgcagaaa aaggcttatt gcaagcttac
     2581 ttgacaggac caggaacgaa cgagttttct gtaaaagctg gaggagcgtt gaatgttgtt
     2641 cctgatgcag ctccttactc tggagaaaag ctttctgaag taaaagaagc attgaaaaaa
     2701 cacgaattcg atttcgaaga acaaggtgaa ggcatcgtcg ttcttggtaa aagtatccac
     2761 gcgaaagatg cagcacaagg agtgaatgct atttctcgtc tggcaattgc tctttcagaa
     2821 gtatttgatt ttggcccaat caatttctta ggaaaattag ttcaagaaaa tgcaactggt
     2881 gaagctgtcg tgggcaaaac agaagatgaa caatccggcg agctaacgat gaactttgca
     2941 agtttggaaa tcacaccaga acaaacaaaa atcggtgtag atatgagaat ccctgttaca
     3001 ttcaagaaag atgatttagt agctaaatta actaaaacag cagaaaaata tgggttgact
     3061 tatgaagaat ttgacttcct tgattcatta tacgtgccac tagacagtga gttagtgaaa
     3121 aatctattgg ggacatatcg cgatattact ggagacatga ctgaaccgtt tgtatcaggc
     3181 ggcgctactt ttgctcgtac gatgaaccag tgtgtggcat tcggtgcgat gttccctgat
     3241 actcctgact ttatgcacca ggcgaatgaa cgatgggaat taagtagtat gtataaagca
     3301 atggaaattt atgcagaagc tgtgtatcgt ttatgtgcta aataaaaata aacatacaaa
     3361 aagggcggat attcaaatgg atattccgtc tttttttatt tgatgctgga aagtttgcat
     3421 ttctctctta tttttttaag gtataatcat acttgattaa gaaaaagggg tcactcagat
     3481 agaaaaggtg ggagaaagtg aaaatattta gacgaatact aattggctta gtcatcattt
     3541 taggattact cggtggagcc ggttattact atatacagca aaatacgtac gaaccatcgt
     3601 cagaagcgca agtgttggta aaagaaggaa aaaacaaaga agattactta ttttttgaag
     3661 gagagaaaaa gaatcatgga aagtctccat tacttatttt ttatcctggt gctctcgtag
     3721 agccagaaag ttatagtatt tgggcgcaag atttagcaaa aaacggcttt tcagtcgcta
     3781 ttgtcaaaat gccacttgat ctagcagtga tgggcggaaa caaagcggat aaggtcaaag
     3841 aagattttcc aaattccgat tatgtgatcg gtggccattc attaggtggc gtaatggcaa
     3901 gtcgttatgt caataagaat cattcagaca aacatttaaa aggggtattc ttcatggcaa
     3961 gttatcccga caaaaaagga acattgacag aatcagctgt tcctgtttta tcgatcacgg
     4021 cgacaaaaga taaagtattg aatcaggaag cttatcaaga agctaagcag tatttaccgg
     4081 aaagaacaga gtatgtatca atcaatggcg gaaatcatgg aggttttggc agctatggtg
     4141 agcaaaaagg agatggagag gcagaaattt ctaacgcaga ccagcaaact gtgatcgtca
     4201 atacattgga aaactggctg aaaaagttga aataatataa atgggtccta tagggtccgt
     4261 gaaaaaacta gtcaattaac tatagatagc aaatgaatct ttcaaaataa gtcgggattt
     4321 cacggaacaa gagaaacaga tcccggctta ttttcaagtt tagagaactt agtgtacgtt
     4381 tttaaagttt cgtttatatt tatattttat tttctaataa aatcgctcga actggacatt
     4441 tttgatacgc tttttttaca tatgtttctt cttccggcat cacgaactgt tgatgagcct
     4501 ctggttcttg ggcaaataaa acgattcctt cttcatcata atcgaaaata tctggcgctt
     4561 cgatttgaca aagaccgcat gcaatacagc gttcaggtac aagtttacat agtagtgaca
     4621 tttctaacca tcctaactta aggagaataa tatgattgtt tttattttat cgctattttc
     4681 atcaggacac aagctgcgga ttagttctct ttaccaactc cttgtgggaa aacgaacgac
     4741 ttcagtactt atttatggat ttacccacga acttttgttt atccataaca gttttccaga
     4801 attgaagcag gataagtttt atcaaatttt gcaaaaaatt gcgcagcaag gttggatcga
     4861 aataaatgaa aatgaagcaa agctaacgcc tgcaggggct gatatgttgt ccgaacatca
     4921 aatagaacat actggtttgc gttttgaccg ctatggcaga acaggggaaa ctagttggcg
     4981 gctcattaaa tttgctgttc aagtaatttc taatctggca acgggtattc aagactatct
     5041 tccagcagag acaagtcctt tttatacttt ccaactaaaa aaatggctta gtgaaagcca
     5101 attgccacgt gaaattttga ttgatactgc gtatgagagt ttggtgcagc tgttttcgga
     5161 gataccagaa gaagcagcag attttttagc taaccaatta tctggaaatg agcgaacagg
     5221 gctgcttcct tatcagctgg caaaaaacaa tgatgagtca gcggtttatc tgcaacaaag
     5281 cagatgtatc caccttttgt tagcacaaat cgaaaaacgg ccagattcgc tttggcacgc
     5341 tttgatcgat tcattattac agcaaaattt caatcaaagc atgatgatca ccaagcagat
     5401 gtttatgaat ggtcagacaa ttgaccagat catggcaatc cgtcatttaa aaagaggaac
     5461 agtaacagat catcttattg agtgggcctt gttttttgat gattttccat acgagtggat
     5521 tctctctgca gaaacaatcg aacgtttgga accaaataag gattccgtgc gagagtggcg
     5581 ctttagcgaa tggaatgtag atggacaatt agattatgga gaatttcgtt tgtatcaaat
     5641 ctatctattg agaaaggaag cgatacagaa tgttaataaa tgaattaaaa aaatattttg
     5701 gatttgattc atttcgtccc ggccaagaag aaattattca ggcgttgctt gatggagaag
     5761 atactctcgc tattttgcct acaggaacgg gaaaaagcct ttgctatcaa ttgacggggt
     5821 atttaatgga aggtctggtc atcatcgtgt caccgctgct ttctctaatg gaagatcaag
     5881 tgacgcaatt acaaaaacga ggggagaaac gagtagctgc tttcaatagt ttactttcgc
     5941 aatctgaaag aagatacgtc ctccgtcatc tatcgcaata taaatttttg tttttgagtc
     6001 ccgaaatgtt gactaatcct tctcttttag agcatctgaa aaaacaaaat attgcgttat
     6061 atgtagtaga tgaggctcat tgcgtgtctc aatggggagt tgatttccgt ccagaatatc
     6121 aacagttagg aaaaatccga aagcttttag gaaacccggt cacactggca ctaacggcga
     6181 cagcaacaga tttagtagca agagatatac gtcatgtact atttgatcga ccaccaaaag
     6241 agatcagaca atcagtcaat cggcaaaata tctcgctttt tgttcggaaa acgcagcaaa
     6301 aagaacaaga acttgaacac ttcatggaaa gagcacaagg agcggcaatc atttattgtg
     6361 caacaaaaaa agaagtcgaa cgattgtatc atttattcag ggaacgtttt acagttggat
     6421 actatcacgg tggtcttgat gcagctcaaa gaagacaact tcaacagcag tttgtaaaaa
     6481 atcagctgca gtttctcatt gctacgaatg catttggtat ggggatcgat aaatctgata
     6541 tccgttatgt ggtccattat gatttgccag acagtctaga aaactatgta caagaaatcg
     6601 gtcgagcagg cagagatcag gaagaaagtg cagcaattct tttttatcaa agtggggatg
     6661 aacgtatcca ttatttcttt aatcaacttt ctagagaaca gcgacaaagt tttgagcttt
     6721 atttggaata tgccacagag caagctccct ttgatgagct gcaaaaaaaa tggatggagc
     6781 tgatccagca gtctgaaaaa ccagaaaatt gggtggaacg gctcaaacgt caggaaaaag
     6841 aaaaagaatt ccgcctgcag cagatgcttc gctatattaa tgaagaaaac tgccgcagaa
     6901 aattcatctt agcttatttt ggcgaaaaac tctccgaaaa gccgcagaat tgttgtgata
     6961 ttgatggagc aaaagcagaa cttctgccaa agaggaagct tttttctaag actgaacaac
     7021 ttcattggga aacgatttta ctaaatttat ttaaaaaaaa catggaacat tgatttttta
     7081 ggacaagaca ctttttccta tggtataata cacacgatag agtttaggag gtaaaacagt
     7141 gagcagaaaa gatagacatc aatctacaga aaataacgaa acacaagaac catgggaaca
     7201 accaatctac gatacagatg atgaagtttc atcaagaagt tctcagagac aacaaaagaa
     7261 aggaaataca ttatttcttt cgctattttt gattttactg gtcttatgta tcgctattcc
     7321 agcaggtgct tatttctgga tcagaaatgg atcaagcaac aatgcaacag cagcaacttc
     7381 ttctgaaact tcgacatcag tagtagaaag ttctacaagt acaacaaaag aggagtcaac
     7441 tgagacttca acagtagaat ctagtacaat cgcggaagaa tctactgtgt ctcaagatac
     7501 aggtgtaacg tcagaaagca gtattgcagc agagacaaca ccgacttcct caagtgaggc
     7561 aacttatcaa gcaggggatt caagcagcaa tgcagcagaa acaacacctt cttcatcagc
     7621 tgcagccggt tcaacaacga ctgttcaagc aggcgaaggt ccaaaacaag tggctgagcg
     7681 tgcagggatc accactgatc aattgtttga attgaatggc ttagatccaa acaactttat
     7741 gctttaccct ggtcaagaat taagaattaa ataataatag ccgcgctttg cggctttttt
     7801 tctgagaaaa aggagttata tcatgacaca aatcagtatt gcaatcgacg ggcctgcttc
     7861 atctgggaaa agtaccgttg ccaagatatt agcaaaagaa ttgaattata tttatacaga
     7921 taccggtgcc atgtatcgcg cagtaactta tttagctatt acgaacaatg tatctttttc
     7981 agatgaagaa gggctagttt cgctgatcaa aaagtatcct atctcttttt cgcaacaaga
     8041 agatggacag catgtattca tagcatcaaa agatgtgact ttagaaatcc gtcaaccaga
     8101 tgtcacaaaa gctgtttctg aagtctctgc acatggaaaa gtcagagaag aattagttgc
     8161 tgtccaacga aaaatagggg aacgcggtgg cgttgtcatg gacggaagag acatcgggac
     8221 agctgtatta ccgaatgcag aagtgaaaat ctttttagta gcaagcgtaa gtgagcgtgc
     8281 agaaagacgt ttcaaagaga accaagaaaa aggaattccg actgatcttg aaacactgac
     8341 gaaagagata caggaaagag accattatga ttcaacaagg gacgtctctc cgctgaagca
     8401 agcagaggat gctgttcgta tcgatacgac tggcaaatcg attccagaag tcgttgcagc
     8461 aataaaagct gttgttttga aaaaaggtta tcttcttttt taaattaaga aaaaaaatga
     8521 aaaacaattg ttttttcaag gaaaacgaac gaaatcgctt tatttttttt gaataatcaa
     8581 ttaaactaaa aacagtaccg tacttacggc agaatgttgg ttaggaggac aagtgttatg
     8641 acagaatttg atcaaaatca agagaacaac cagtcaatgg aagacgcaat gaaaagcgta
     8701 caagaagtaa aagttggcga tgttgttaag ggcgaagttt tagtcattga agacaaacaa
     8761 gtgattgtcg gaatcgaagg ggctggagtt gaaggagtcg ttccggcaaa agaattatca
     8821 actttgccgg tagaagatat cagcgaagta gtaaaagtcg gtgatgtatt agatttagtc
     8881 gttatcagta cgatcggcaa agataaagaa aacggcagct atctactttc aaaacgccgt
     8941 ttagatgcta aaaaagtttg ggaagatatc gaaagagatt tccaagaggg caaggtcatc
     9001 gaagcacctg ttacaaatgt tgtcaaaggc ggtttagtcg ttgacgtcgg tgtacgtggt
     9061 tttgtccctg catcaatggt ggaagatcat tttgttgcag acttttcaga ctataaaggc
     9121 aaaacattga gctttaaaat catcgaaatc gaaccatcag aaaatcgttt gatcttatcg
     9181 cataaagcgg tagtggaaaa cgaaagagaa gaaaagaaaa aagaaattct ttcaactatc
     9241 catgagggag atgttctcag tggacgtgtt gcacgtctta ctgattttgg cgcatttgtc
     9301 gacctaggcg gaattgacgg tctagtccat gtctcagaga tttcacatag tcatgtagca
     9361 aaaccaagtg atgtattggc tgtcaatgat gatgtaaatg tcaaagtgtt gtctatcaat
     9421 cctgaacaag aacgtgtttc cttgtcaatc aaagacacac agcctggacc ttggtcagaa
     9481 atcgatacaa aagctccagt tggcacagta ttagacggaa cagttaaacg tttgacaagc
     9541 ttcggtgcct ttgttgaagt gttccctgga gtagaagggc tagtccatat ctctcaaatt
     9601 tcacataaac atattgcgac accacatgaa gtgcttcatg aaggtgacga agtgaaagtg
     9661 aaagtattag agatccatcc agaagaacat cgtattgcat tgagtatcaa agctttagaa
     9721 gaaaaaccag cttcagaaga agaaccaaaa aatgttgagt cttatgaatt accagaagaa
     9781 aatactggat ttactatggg agacatttta ggtgatgcgt tgaaagaaga agtagaagaa
     9841 aaagcaaccg atgattctga aaaataattg attttgtatc tgtgactgag atgaaagcag
     9901 ctgcttttgt ctcagttttt cttttgaaaa gcgattagta tttcaataag gaatggctct
     9961 tcgtcaatga gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga
    10021 cagctatctt gtctagccgt gttgttgttc tggctttagt cagttgggta gtgttcttag
    10081 ttttcttgaa atattttcct cgatataaaa agattcgttc tcggtagttt tgaaagtttc
    10141 taaagccata ggcaattcgt tttagtactt tgatatggtt gttcaaacat tctatagggc
    10201 cgttggaata gggaactcgc aacgctcgtt tgatttctgt tcgatatttc ttgaaggtcg
    10261 tgaacactgg ttgatagtgc tctggcaact ccttgtagtc ttcttttagg agcgcgacga
    10321 attcaggaac atcttgtctt ctgatagcgg aaagaaaaac ctggtagact tcgtagcctc
    10381 gctttaagga gtcatcataa tataatagcc gatcgactat ctccgcttct gtcagatgat
    10441 ctcgaaatga cggacgccag tagcgatgct gaccattcaa ttcccacgca ttcttttgaa
    10501 gtagtctcca gtatttcttt aagtgcttcg accgacgatc ccctttttta aaggtcttca
    10561 ttacttgaat tcggtggttg gtaaaggccc gaccgatgtg ttggctaata tgaaagcgat
    10621 cggcgacgat tcgggcattc ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga
    10681 aatctgaaac cactagacgt acttgttcac ggacttttct agggtagcgc aagaaatagt
    10741 cgcgtaaagg attgagttgc cgattttcca caatatcaat cagttgatgg gtcttgccgt
    10801 ccatcatgac aaagctcatg gctcccgaga cgtttttcac cgatttgaat tcatcgaaac
    10861 acaccacttc tggtaacggc tggttcaaag aaacggtttt aggccgaagg ctgcgaagta
    10921 ttcgaatgac agtggtcgga gaaatgtgct tcatacgtgc aatcaaagac atagaaatag
    10981 gctcacttaa tagctcaaga atggcttgtt tgactctgcg ggcaatggag cagcgacgat
    11041 cgctaactga atcttctgct aaaaaagtct ttccacagtg tttacatttg aagcgctgtt
    11101 tgttaatccg cacaatggtc ttgtagttac tgacatcatt caataagatg gatacttttt
    11161 tccagcccca tttaatgatc gtttgcttat tttgatagtg gcaatggaag cattcctctg
    11221 gttgataggt caatgtgcca tcaaaaacaa aacatgtttc gtcattaatg cgctctttgc
    11281 gaaaggcatc ttcatgaaag gtaagattta ggtctagaat atctagtaac tctttggtaa
    11341 actctgaata ggacatcttg aaacactcct ttgaatggtt ttcgtcgact ttattctaaa
    11401 ggatttcaag gtgtctgttt gcgtttatta gaaaaaagaa aaagtattgg caacagaagg
    11461 cgtcaccaac actagatatt atagatccct tattttcttc gcttcaaatt gtcaaggagt
    11521 catcataaat tttgtatgga ttcttttgtt tgggtagtag tcttctaaat aatcttggat
    11581 taacaccttc atctctattt tacttttata tttgttggag tcgattaatt ctcatttgat
    11641 tgatcgatga aacgcttcaa ttactgcatt gtcaacagga gtcccagttt ggctcattga
    11701 atgtgtgatg tggtggtcgg tcaacagctc ttgataaata aaagaccgat gctggctacc
    11761 tctatcggaa tgaattaccg taggcaattc tccttaataa atttgtaatg gacggacgac
    11821 aatctctttt ttcatgtgtg aatccaccat atagtaacgt agaatacgag ttgctaagtc
    11881 aatataggtg gacaaataga ggcgaccgtc attacatgga atgtaagtaa tatccgtcac
    11941 ccaaacagca tcaatttggt tctgttcaaa ttatcgattc aacatttttt ttaccatttc
    12001 tttgattgtt ttttctctac catgagaaga tttgcgtcga tgaaatcctc ttgtgcataa
    12061 attcatttca cgcataagaa cagctacaac acgtttattg atttcgattc cttcatcgta
    12121 aagtttttga gtgatccctg gagatccgta tcgatctttg tgttcgtaga acactcgtct
    12181 tcctctttta gtcgtttatt ttcctgttca agaatgtaga aatctgcttt atcaggtaat
    12241 ctttttcctt gtccagcaaa agcatttttc ccgtatcgac gatattctga aacccatcgg
    12301 gaaattgttg gacgagagat attgtattct tcagcaattt tcttggtagt tacttttttc
    12361 tcaaggatat cctgtgaaat ctttagttta aatttacgat catatgtttt tttcaataga
    12421 gttcattcct tccggtaacg taactgatag tcaagaaaag tgtatcatag cccaacttag
    12481 tatcagggtt tgtcaactaa actgtggaag ttgatagata agagttttta acaaccaaaa
    12541 ttctcttgac tatttttgaa atcagacaaa ttttgattgg acacatagtt gaataaatct
    12601 accgtcgtta cggaaggtaa atcgcatgct tttcaattct agaggagaag tcctcgttga
    12661 agtaccatgg cccagttctg gattcgacct cctgcccact tcgtacgtaa ataaagtaat
    12721 tttaggagtg agactgtgac aaaagttatt tttaagctaa aaacgaatta aaaaacgtta
    12781 ataattaaaa tcat
//
LOCUS       DAHEYX010000067        12711 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHEYX010000067 DAHEYX010000000
VERSION     DAHEYX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.068"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(205..1515)
                     /locus_tag="MB219_001322"
     CDS             complement(205..1515)
                     /locus_tag="MB219_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCC8427046.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1527..2945)
                     /locus_tag="MB219_001323"
     CDS             complement(1527..2945)
                     /locus_tag="MB219_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8427047.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3185..4753)
                     /locus_tag="MB219_001324"
     CDS             complement(3185..4753)
                     /locus_tag="MB219_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCC8427048.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4757..5167)
                     /locus_tag="MB219_001325"
     CDS             complement(4757..5167)
                     /locus_tag="MB219_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCC8427049.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5164..6087)
                     /locus_tag="MB219_001326"
     CDS             complement(5164..6087)
                     /locus_tag="MB219_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCC8427050.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6084..6962)
                     /locus_tag="MB219_001327"
     CDS             complement(6084..6962)
                     /locus_tag="MB219_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCC8427051.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6949..8286)
                     /locus_tag="MB219_001328"
     CDS             complement(6949..8286)
                     /locus_tag="MB219_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCC8427052.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8289..9392)
                     /locus_tag="MB219_001329"
     CDS             complement(8289..9392)
                     /locus_tag="MB219_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCC8427053.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9466..10974)
                     /locus_tag="MB219_001330"
     CDS             complement(9466..10974)
                     /locus_tag="MB219_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCC8427054.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10940..11632)
                     /locus_tag="MB219_001331"
     CDS             complement(10940..11632)
                     /locus_tag="MB219_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCC8427055.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11654..12628)
                     /locus_tag="MB219_001332"
     CDS             complement(11654..12628)
                     /locus_tag="MB219_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8427056.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
BASE COUNT         3781 a         2732 c         2065 g         4133 t
ORIGIN      
        1 tgaagttttt ttcgatagaa tcaagcttcg caagtggttc tttttaacga cttagttatc
       61 ttatcacgtc taaaaacatg tgtcaactct tttttggaat tttcataaaa tgtttcaatg
      121 aagcgcgaca acgttgttta atatatcatt aaaaaaaata aaggtcaagc taaaatatta
      181 tttaagcttg accttattat aatttcaacg attgtcaaaa gtattgtttg cagctgtgtt
      241 gccagtttct atagataaat cacagctttt gaaacataag ttttgtactg cttcgattcg
      301 cccgaattct tttgcagtta tttggcaatg agaaaagcag acatcttcta taggcttttc
      361 aggaaatgct actaaatcaa aagctcggga aggaagtgag taacctggag aaaggcatgc
      421 ctgtacgttt ttgatgtgta tgtctttgac tttcgtcatt tgctttgatt caggtatttc
      481 ttcagccact gcttgccaac tttctggcat ttgagcaagg tcattgctat tttttctatt
      541 gtattcatta tgccagtcca ttatccaaga aaaaggaaat tgtacatcaa tcatattcag
      601 attttctacc ctgatatttt cgatcactcc ccctctttct tttgaagatt tcattcgaat
      661 accacagtct gactgaaaaa aatcgatatc gtgaatatat acgtcactaa ctccagcact
      721 aacttcgcta ccaatagtta caccgtatcc tgaacggatt ttacatctag caacctcgat
      781 tcttgatgat tttcgattga ctcttgcacc attgccatct ctgccagact taatagcaat
      841 acaatcatca ccacaggcca aatcacattc atagacacgc acatttgttg aagaatcaat
      901 atcaatccca tctgtacttg gtccatcatt gttgcggatg attacttttt ctactaatac
      961 atcgttagaa taagtgattt gcaagttcca aaaacctgat ttctgaaaaa tcaaatcacg
     1021 aacaacaaca tgttctgcat gatacagtaa acaagcgcgt ggacgtttaa tagcataatc
     1081 cgcaatccaa cgaagtccct tttgatcgta ttcagctctt gtcccgcttt tttgatcttt
     1141 tccccaatac agctcccacc aatggtctcc ttgaccgtcg attattcctt ttccttctat
     1201 aagaatatct ttcgccgaga gaacattcaa aatcgctgct ggccatttca tttctacgcc
     1261 ggctactcgt gtatcgattt ctggaaatgc ttcgatatct gttctcccct tgaggactgc
     1321 accttcttca aagataagat gactcttatc ttttaaaaaa agggctccga ctaaataaat
     1381 gccttttgga ataaatacag gtaagtgttc tttcgcacct tgatcgattg ctttttgcag
     1441 tattattgta tcatcttgtt gagtgtcgtt tgttttaaag ttcaaagcat tgataatccc
     1501 tttttttgtc cgcaatacgt ttctccctat ctttcattga tttttttcaa ttcttcacca
     1561 ataaatacag tttttgaacg attcagtggg taacctacta gcaaaatgat tgcggataca
     1621 aataagcaga atgctggtaa caagtttgcc atcgtataaa tcctttcttg tactaaaact
     1681 gattggatgg ctccacctgt tgtagacgat tggtaaccaa tgagtgccag cataaatccg
     1741 cctaccgctc ctgcacatgc ttgtcctact tttctcgcaa aagaatttac tccatatact
     1801 gttccatctt ctcttaagcc tgtcttatat tgatgataat caatcacgtc agtaatgaat
     1861 gcccagacca tcaaattgaa taaaccagat cctaaagtag cgataaataa aagaatcaga
     1921 tatacccatg catttgttat atgaaggaaa tacatcagca agtacataca tgaagaagca
     1981 agtaacgcaa atacgcttgc ttccttttta ccaaataatt ttgtcagctt tgtagcaaat
     2041 ggtgctaaaa ctactactgt tccgtaagta aataaaagag caacagacat tgcttgttta
     2101 ttatggaagt aatcattgaa taaataagtc atattcgtcc cagctaaaat ctgattgatc
     2161 acaatgaaaa tgtcaacaat gactaaaata atcagtgctc gattagctaa caaacctttt
     2221 accaactgag aagcagatac tttttcgttt ttgacaactc ttactctttc agtagtcaat
     2281 ttacaagtta acatatatgc gacaaatgct attgcagcac atccgattga aatccaaaag
     2341 aaatgattcc ctgaaacgac ttgttgtcca ctcttgttat gggtatacat taaaatcgga
     2401 atgaagaatc ctgtagttga tccgccaatc gcagaaccaa tgcttcggaa cgtggacaac
     2461 gaggtgcggt cattaggatt tgagctaatt gctgctgcca ttgctccata cgggatattg
     2521 attgttgaga gaaaaatccc ataaacaatg tatgaaacaa aaatataagc aatcttcacc
     2581 gtcattggaa gatgttggac gaatggcaaa aacaaaatga cagtcactaa gcaaaatgga
     2641 tacttcattt ttctgatcca aggaataaat cggccagttt ctgttaactc tgagacatca
     2701 cataatcttc caaccatcac atcagcaaac gcatccaaaa ttctcgaaat caagaataac
     2761 gtacctacga ttgcccctga aacgccaaga acattcgtat aataaatcat caagaaacta
     2821 ttgactaaac ctaaaataaa acagttacct agatcaccga acatgtagcc tatcttatcg
     2881 gaaatcccaa attccttttt ttcttttttt attgtatttg ctgtaacagc attttgtgaa
     2941 agcatttgaa ttcctcctac ctcttctagt aaacgttttc aaaattataa ctcttttttt
     3001 cttgttttta tattcacaat gttggctttt tattgcatta tcttgacaga taaaaaacaa
     3061 aaaataatct aaaaacattg atgcataaga ttgataactt tttttacata acaatatata
     3121 caaacaaaaa aattcactaa ctagtgaaaa taagttagtg aacctcattt attaaagaat
     3181 taaattacag atgatctttt ccatcatact tgaaaaactc aaaatcagca taacttcctc
     3241 tttttttatt tagatcatgc actgctatcc cgataaaatt tccagtaaac cctcctgaga
     3301 gtatacacat gtttttttgc ggacctactt tcttccattc ttcagttgat acgtctttca
     3361 aatgaaactg tgctgtctct ttctttacag tgactttcaa aaaagtcttc ttttctgtga
     3421 gtggaatcgt ccaatcttct atttgtgcta ctccgttttc tgattctatc atacgtaggc
     3481 attttccctt gtcctcattc caagtaacat aacaataaat ataattggat tcatttaagt
     3541 acagtaccag tccagccatc tgattaaaat tattaggcat gtattcaaat gctgtctccg
     3601 catcaaaagt aaaatccgtt tgccgaatag ctaacaaatg atgttcgaag gtagattgaa
     3661 gagattcacc ggaaatcaga cgtaaaaaac ctggtctaca ttttgtatca caccaatcag
     3721 catttggcaa aatgcgtctg gcattccagt tcttctttag ctctccttca aatgtatcca
     3781 taaactgatg atctttttct tgttgtacct cttcatttgt ctcaatcatt atttcttcca
     3841 tcggagttgt ctggctatct ttaagtctta accaatcgtc ggacgtccaa tacacttctt
     3901 gaatagcggt ttctcttccc aaaatagccg caccgtttaa tggtctggta caaagatatg
     3961 tcatgtacca tcttcctgac ggggtttgta ctaagctccc atgtcctgta cattgcaatg
     4021 tagaagcagg tttgtcacta gctgtcagca taggaaaacc aggatcaagc tcgtaaggac
     4081 cagtaatatt ctttgctcgg caaactgtaa ctgaatgtcc tcttcctgta ccgccttctg
     4141 ccgtcatcaa ataataatac ccgttacgaa aatacaaatg tggtgcctcc gttttcgcta
     4201 attctgtacc tgcaaatatt ttatagatgg gaccatcaag cttcttcttc tctgcatcat
     4261 attcttgtaa tacgataccg ctggatttat tcggtgtgtc cagacgataa tcccatagtt
     4321 cattgacaag ccattttttt cccgtttcaa gatcatggaa caaagaagga tcaaagccgt
     4381 tactatttaa ataaattggt tcggaccaag gacctgtgat ctcagatgca gtgatcaaat
     4441 aattgtggac atctttaaac ggacggcctg cacttttgac atctgtgtaa acaagataaa
     4501 acaatccatc agcataactg atctgtggcg cccatatact tccatttgta ggatttcctt
     4561 gcaaagatac ttgagtggtc aaaatatccg ttgtatgtga ccagttgact aaatttttcg
     4621 actcatatac tcggattcct ggaagccatt caaaagagga gacaataatg taataccgct
     4681 ctttcactct taatatatta ggatctggat tgaatcctct taaaatagga tttttcactt
     4741 tgatagtctg catgattcag gctccttttc tcattcttac tcgatataaa ctgaatgccc
     4801 cagctgcgaa ataaaaaaat gcaaaaggaa acgcaataaa ctgagtgcca atcaacaata
     4861 tgaatcctgt tataaaatac aaaccagtgg cagttgttaa gtattttcct ttagcagtca
     4921 aaaacaatgc tactgctaaa aaagcaagtg aaataaacaa cacaacggca aacagaccag
     4981 tcatgtcctg taataatcca aatgtatttt ctaaagacaa agttgaatga tctacagcaa
     5041 gcgactgata acttttttta aactctgacg cagacatatt acttaaagta actgctaacc
     5101 cgccaaagaa aatcaaagat agactgccac cgaccattcc tagcaatagt tcccattttc
     5161 ttttcatttc tgaaccccct gcttttctaa atgtatccat tcaggtcttt tgatcgatgt
     5221 ttcataattg ttattacgtt cagagaattt gacagtctgt tcatttttct tgtcatccca
     5281 attatcccaa ccagttgggt gaacatgccc acctatctta cacgcttcaa ataagacatt
     5341 tgcatacgga cgccacggtc ttcctaaata gacgtttcta gtatcaggtt ctgcagtcaa
     5401 ggtacagctt tcaaaacgat accctgtttt ttgatttttc ggcgtagatg ctgctgtgat
     5461 gtatccagga tttttactgg ctaagctatg gatatgacac tgatgaaaaa cggcatctgc
     5521 cccaccaaat atgaaatcca ccgttccttc aatccagcaa tattcatagg tctgacgaca
     5581 atactcagga ttggcagaag ccggtgtaca aaaaggagta ccatcttttt gcaaatcagg
     5641 caacggaccc gtgcataagg tatcctgata accagatagt cgacagtttt ttaaagatac
     5701 gcgatctcct gaattgaata aagcaacagc ctgtcctacc ttttctcctg gacctgctgt
     5761 attttcgatg tgaagatttt ctaatacgac attcgatcct tctaagaaaa aagtagcact
     5821 tcggaaagtc cctaactggc taccgtcaag atactcctgt tttgcaaaac gattaccgga
     5881 gataactact gttcctgctc ccacaagttt aaaattggat aatctgcttt ctatatattc
     5941 atcatatctt ccttctttta ctatcaagac ctttggatgg ctatccggca ggcctgccaa
     6001 ataatcgatt ccttcttgga tagtttcaaa atccgtcggg gtatctatag cattctttcc
     6061 aattatcagt ttgtctttca tcctcataaa tgctccctta tgaattgttt ccaaatattt
     6121 ctcatttctt gtgtttccat atgaccgcct gttaaagaaa aagaagaaaa gttttctgga
     6181 ctacccattg attgatactt tttgtccagc ccttttctca gcttcagtac gccttcgtcc
     6241 aagcacatcg tatcgttttt tccaactaag ctaagacgag ggcgcggagc gattttttct
     6301 tggattgcta acgttgtata agctgtcaat agatttggca catagtaata aaacccatga
     6361 tgatctaggc ggcgatgtag caaaagtatt tcgatgtcca cctgtgccgc tagatcaata
     6421 caaactttga cacgagaatc caatgctgtc acccaccagc ttaataagcc tcccatagaa
     6481 agaccaatcg ttgcgagccg ttgagtatct gtgtcttccc ttgtctccaa ataatcgatc
     6541 aagctgatca catcataaat acgcattccc cataacgttt ttcctgttaa taaaaattgt
     6601 ttgaacaact cgctttcaga aatacctccg cgttcttcaa atccccaagc atcaatcgac
     6661 caaacagcat atcccattcc tgtcagtact tctgcaaaag atggagaagc taagtaagga
     6721 gcaccatgga tcaattcact tttcccgact gaaaaatctc cgccgtgaga atgcaggtaa
     6781 aggaccggcg gaaacggtcc ttctttttct ggataagcat agagggctgg tactgtttcc
     6841 aattcattta agtacaattg atagtattca agcataaaac cgttttgttt ttctcggtat
     6901 aatcgttttc cactaggggt atgtctgcta gatggagcac ctaaaaaatc attcaactgc
     6961 ataagcaaag tttttacgaa caaccagttc atccatattt tctgattgat ttgattggaa
     7021 tttctcaata ctgatttctt tagtattttc taatagaaat gctggtccct cgtgattttc
     7081 aatcgacatc tggttaaagg aaaccttacc cgcaaagcca atataaaatc cttgtctgct
     7141 catttcctct attcctgtca tcatagcagg aacaccggga actgcatctt gggccatcga
     7201 gacatcgata ttgtcaaatg tgatttcttg tatgaattgt tccgctaatc cataaataaa
     7261 acctgcagaa gcatggacat tccttgccgt gatattcgaa aaatggatcc ttctgaatgc
     7321 tggtgtccgt tcatctatcg gataagcctt tttttcccat acatacggtt ctttccctcg
     7381 cggaccacag aaataataaa gattcagtat aaatggacac atcacattat ccatgactat
     7441 attggatact cgaatatctt caacgatccc gcctcgacct cgtctagatt tcaatcgtat
     7501 tcctcgatca gtttcttgga agatacaatt tgaaatggta atattacgaa tactaccact
     7561 catttcactt cctaatacga caccgccatg tccatggacc atcgtgcaat ttgtgatcgt
     7621 gatattttcg caagcgatcc tttcgtacgt atcttcagtc cctgccttga ttgcaataca
     7681 gtcatctccc acgtcaatat ggcaattact aatacgcaca tttttgcatg attctggatc
     7741 aattccgtct gtatttggtg aatccgctgg gttcaagatc gtcaagttat caaacgttgc
     7801 attactgcaa agaatcgggt tgatcgtcca acttggtgac tggatcaatt taatatcttt
     7861 cacagtaatt cgatgacagt tatggaaact catcaattta ggacgaggat aagcgagatt
     7921 atctggttca tttctaaaag tatgccacca tttttttcca tttccatcta atgtaccgaa
     7981 accagtgaca gagatgttct ctacattttg agcatagata caggaagcat aaacttttcg
     8041 atggactcct tcccaacgtg aatgaacaac aggataatct tttggatcgt cagagaattt
     8101 cagaaccgct cctgcgctta aatgaagttc gacattgctt tttaaaaata aagctcctgt
     8161 tagaaattca cctgctggaa caactacggt cccgcctccg tcacttgctg cagcatcaat
     8221 ggcttgttgt attgcttcag tattcaattc atcaattgat gcgccaaatg tcaaaatatc
     8281 atacatattt ataatgcctc cacttcaatc aatgcttgca agaatgcacc aacgcctttt
     8341 tgatcattac aaataattgg ttcactgatg tagtaagcat atgaaccatc tcgctgatct
     8401 gctccgccta accctgctac ttgacagttt ttattcaagt tcagccagcc ttcttttgtt
     8461 tccagaacaa attcatcaag taatccttga tgtgatttct tcacttgtgg aagccattct
     8521 tctttgttca gcagtcccaa acgtattcct ttagccattg cgtaaacaaa catactactg
     8581 ccggaagctt ccagataatt tcctttccgt tttgtttctg ttgttacttg ataccatgtc
     8641 ccgctgtttt cttcttgata tggaagtaat gcagccagtg tcttttctag aatttctttc
     8701 agcaaagaac aatctgtttc ttcatgaagt aattcatagg tatctgctgc agccatcaaa
     8761 taccagccaa tcgatcggct ccaaaaatga tgggatagac ctgtttttgg atcagcccat
     8821 ggttggacct tcttttcatc ccatgcatga atcagcaaat ttgtttttgg atcgaataga
     8881 tgtttataac tgatttcaaa ctgtctcaaa acatcttggt aatctttttc ttcactaaaa
     8941 ttctggataa attcagcata aaaaggagaa cccatatata agccatccaa ccaaatttga
     9001 taaggataaa tttctttatg ccaaaaagct ccttcggatg tgcgtggatg gtttgccaac
     9061 tgctgggaaa ggagttctgc tgctttttta tatttttctt cgcctgtttc tttgtacaat
     9121 aaaaagaaaa gctttcctgt atttacatgg tcgatattgt attcttcaac gctgtagcca
     9181 cggatcgttc catcttcttg cacgaaatag tcgatattgt cttttatgaa atcaaaataa
     9241 attttttctt gggttcgttc ccaaagtaat tggaaccctt taagtacgac gccatagtca
     9301 taagaccatt ttcctttata ccacttttct tcatataagc gaggtgttct ttccatgata
     9361 ctttttgcta atttctttga ccagttttcc attatttgct ctcctttaaa aattaaaata
     9421 ggatttggga caaataccaa gaagtatttg tcccaatcgt ctatattact gtgccgctgc
     9481 tttgtatgct tcctcgtact cttgtgtgat tcgatcgcct ccttgtgctt tccagtcttc
     9541 tacagctttt ttgaatccat cttcgtcaag ctgtcccatg atgtattgca ccgtagcatc
     9601 aatgacgatt ttttcaattt ctgatcctac ttcattgttc gtcggtgaat ccaatggcac
     9661 agaaggatcc attacagcaa attcttcatt ttctttgatc aattgattag agaattcttt
     9721 ttcagggtct gtatctttga aagagaacgt cacttcacta ggacgagagc ttgataacgg
     9781 ttgcacctct tgttgccaaa gatccatatc gataaattcg atagaaccat catcattcaa
     9841 ttcataatgc gttccttcga taccgccagt catcaattta tacacatctt cttctaaaag
     9901 atcatcaaca aattgaagta atttcttcaa ttgtgcttcg tcttttactt gagatttggg
     9961 aaatgctagc aagccgccaa taccatttcc ttctgaccaa acatgataac cgccgccttc
    10021 accatctgag atcttgttta caggcactaa ttccatatca tcttgaatac cttgacccat
    10081 cgtcttcaga ttcttgatat ccaccattcc agtgtagatt ccagttcttc cttgtgcaaa
    10141 ttgttcttgc tgatcagtct tttgggtaac tgcaaagtct tgtgccaaat agccattctc
    10201 aaatagatct cttgaaaaat tcaaagcttt catatactct tctgtatcaa attccggagt
    10261 gaatttgcca ttatcgtcta cagcccagcc atttggtgct ccaaaataag aagtgaaaag
    10321 tttaaagctg gtataacgaa tatcactagg cgcacgatcg ccaaaaccaa ctgtgtcgtc
    10381 cacaccattt ccatcaggat cgttttctgt aaattgttta gctacttcat aaagctcatc
    10441 catggtagtt ggaacatcta gtcctagatt atccaaccag tcttttcgaa tgactaagcc
    10501 agcacgagcg tagtcttttt gtatcggtac gccgtacaag gcaccgtcga tactcgcagc
    10561 ttcgattcta tcttcagaaa tttgacttaa atttttatag tcttttaaat attctgatac
    10621 atcccagaat acgcctgatt ttaacgcatt gcggacagaa gagtttttca acatcgcaat
    10681 ttgaagtgta acgatatctc ctagttcatc agaagccaat gcagttgtta atctttcttc
    10741 ttttgaagca tcaggaatcc agttgaatgt cagttccgta tcggtttttt cttcgatcaa
    10801 gttcacaatc gtgtctgtag gcggtgaagc atgatgcaaa atatttaacc aagtgatttt
    10861 agcttttcca tcttctgtct ctgaagaact gttacttgaa ctgctgccgc atgcggcaag
    10921 tgctagaatt cctgcaaaac taagacctaa ttttacgagc gttgcttttt tcataaattt
    10981 tctccttcac tgttttaata atatttttgt tttcttctgt tttttctgta tatgagttct
    11041 tccattccaa atggcaaaat atagaatttg ctgtccacgt gactgcggaa aagaagagac
    11101 tgccaccgat aaaggttaaa acacctggca ttgtttggat gatcatcaga taaatcccta
    11161 ccatacttaa tatcaacact aatgagtaat gaagatgtga gaatccgaat aaaaatgcca
    11221 ttttcatttt gaaaaaatat cctttccaat cataatgagc catgattggg aaaatataga
    11281 gtgctgagag cacatatatg aacgcagcaa tatacaaagc gatcctaaga taccaattgt
    11341 gtatgaaaga aagatccaca agtaaaacaa tcccgatggc gccatatatc cagctcatca
    11401 gcaaggtttc acgatagttt tctttaaaat atgtgaagta cgttttcgtc aaaggaaacg
    11461 tttcttttga gcgcagccat tttctaatca ctgcaaccat cgcatacgat gcaggaccga
    11521 aagtaaaaac tcccaaacct gcgatgacta aaccgcccca cagaaaattc aaataaacca
    11581 actgtaaaat acgatttagc cttccgttta ttttatctaa tgactgcagc atatctttcc
    11641 ctctctttct atcttagtaa acgccgtctt ctcctagctt tttggcaatc ttattagcta
    11701 aaattaccat aaacaatcct acgacccctt taaacaatcc cattgctgta ctgaaactta
    11761 actgtccatt cttcaatcca gctgtataaa tataggtgtc gaaaatttct gcaacgcttc
    11821 gattcaatga attcaataac aagtacatat gctcgaaccc taagtctaat gtctgaccga
    11881 tttttagaat cagcaaggta atgattactg gacgaattgc cggcaatgta acatgccatg
    11941 ttttgcgtaa gcgtcctgct ccatccattt ctgcagcttc atacaactgt gtatcgactg
    12001 cagttatcgc tgaaaggtag atgatcgtag accagcccaa ctctttccag atcacttgtc
    12061 cgatatatac tgtgcgtgtc cattcagggg atgttagaaa actgattttt tcaaatccca
    12121 ttgttgcaag gaattcattc aatacccctc cgtcaacatt caaaaataca taagtaatcg
    12181 aaacaatgat tacccaagac atgaagtgag ggatgtaaat gatcgtttga atcccgtttt
    12241 tcaatcgttt gttcgttact tcatttaata gtaaagacaa aatgattggc atcggaaaaa
    12301 acacgaagat atttaatccg aacaagatca acgtatttct taataatgta aagaatgtcg
    12361 gttctgtaaa caaacgaatg aaatgtttga aaccgaccca aggacttccc attattccta
    12421 aaaatggttg ataatcttga aaagcaataa cgagtccccc cataggaaga tacctaaaaa
    12481 tcaagaaata aaggactccc ggcagaatca tcacatataa aaatttattc gttttgattc
    12541 ctgcaattat tttttttcgt cttctgagct tttcttcttt tttcgatagc gtcagttgaa
    12601 atggttcttg tttctttaca ctttccataa gcccctcctt agttttgtga atgtttgctg
    12661 cttatgactt catgatagat aactatctga attacgtata taatcatttt t
//
LOCUS       DAHEYX010000068        12436 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHEYX010000068 DAHEYX010000000
VERSION     DAHEYX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.069"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1472)
                     /locus_tag="MB219_001333"
     CDS             complement(<1..1472)
                     /locus_tag="MB219_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCC8427057.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCA"
     gene            1631..1822
                     /locus_tag="MB219_001334"
     CDS             1631..1822
                     /locus_tag="MB219_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427058.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2025..2942
                     /locus_tag="MB219_001335"
     CDS             2025..2942
                     /locus_tag="MB219_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCC8427059.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2935..4008
                     /locus_tag="MB219_001336"
     CDS             2935..4008
                     /locus_tag="MB219_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427060.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4156..4674
                     /locus_tag="MB219_001337"
     CDS             4156..4674
                     /locus_tag="MB219_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427061.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4655..5047
                     /locus_tag="MB219_001338"
     CDS             4655..5047
                     /locus_tag="MB219_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCC8427062.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5123..6058
                     /locus_tag="MB219_001339"
     CDS             5123..6058
                     /locus_tag="MB219_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCC8427063.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6191..6970
                     /locus_tag="MB219_001340"
     CDS             6191..6970
                     /locus_tag="MB219_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCC8427064.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            6963..7280
                     /locus_tag="MB219_001341"
     CDS             6963..7280
                     /locus_tag="MB219_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCC8427065.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7524..9134
                     /locus_tag="MB219_001342"
     CDS             7524..9134
                     /locus_tag="MB219_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCC8427066.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTIQSTM
                     LQSLNHHRFAIRLTVEAIILKVIFEVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9291..10139
                     /locus_tag="MB219_001343"
     CDS             9291..10139
                     /locus_tag="MB219_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCC8427067.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHVDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10370..10849
                     /locus_tag="MB219_001344"
     CDS             10370..10849
                     /locus_tag="MB219_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427068.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10882..>12436
                     /locus_tag="MB219_001345"
     CDS             10882..>12436
                     /locus_tag="MB219_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8427069.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKVSNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDPDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEFPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPSFQGKTPEVRELHMQVMPIVESLS
                     RQILELLDNE"
BASE COUNT         4031 a         2194 c         2524 g         3687 t
ORIGIN      
        1 gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc
       61 acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg
      121 attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca
      181 tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt
      241 gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact
      301 gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat
      361 tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca
      421 tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata
      481 atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct
      541 gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc
      601 acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata aacagattct
      661 ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta
      721 tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct
      781 tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc
      841 catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga
      901 tacagttggt tcaaggaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca
      961 acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt
     1021 ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt
     1081 ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg
     1141 acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata
     1201 tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg
     1261 caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct
     1321 tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc accaaattct
     1381 aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata
     1441 tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat
     1501 tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga
     1561 aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg
     1621 aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc
     1681 gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac
     1741 ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac
     1801 aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta
     1861 tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt
     1921 ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa
     1981 gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata
     2041 caaatgcaaa ttatgagcca gacgaactag aggagacgaa tgcggatcag gatacaggac
     2101 aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat
     2161 tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc
     2221 gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga
     2281 tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg
     2341 aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg
     2401 atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg
     2461 atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg
     2521 agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa
     2581 aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa
     2641 aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat
     2701 taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga
     2761 agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa
     2821 tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga
     2881 attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat
     2941 gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg
     3001 aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg
     3061 ctagaggaaa caaaaataca cgaaaaagaa aaagtaactc cgacgaatac gaatactgca
     3121 gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa
     3181 gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt
     3241 atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt
     3301 atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg
     3361 tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac
     3421 acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc
     3481 ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta
     3541 aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc
     3601 cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa
     3661 ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta
     3721 gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta
     3781 ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac
     3841 gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa
     3901 aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc
     3961 tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg
     4021 gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat
     4081 aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga
     4141 gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct
     4201 tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt
     4261 tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct
     4321 attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc
     4381 tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat
     4441 ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc
     4501 gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg
     4561 tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc
     4621 gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct
     4681 tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc
     4741 ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa
     4801 tggtcaaaac ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt tactgagtat
     4861 tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca
     4921 ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac
     4981 tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa
     5041 actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag
     5101 ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat
     5161 tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag
     5221 tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat
     5281 tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg
     5341 tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg
     5401 tggggcactt ggggtgatgg aagcttttga aaaattgacg catccatccc atgaagtggg
     5461 taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt
     5521 acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt
     5581 tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt
     5641 aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc
     5701 atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct
     5761 agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttgtcaaa
     5821 aatcaagtta gcctttgaac gacctgaaat cagtcggcta attgatatca aaacagttca
     5881 tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa
     5941 tgaagcttac tcggtcatca acgatattga aaaaaatatg cgtcagtctc ttcctgacaa
     6001 gaagatgtat atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa
     6061 cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg
     6121 gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg
     6181 agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat
     6241 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
     6301 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
     6361 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
     6421 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
     6481 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
     6541 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
     6601 ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt
     6661 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
     6721 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
     6781 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
     6841 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
     6901 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
     6961 ccatgactaa attatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc
     7021 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
     7081 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
     7141 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
     7201 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
     7261 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
     7321 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
     7381 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
     7441 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
     7501 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
     7561 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
     7621 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
     7681 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
     7741 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
     7801 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
     7861 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
     7921 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
     7981 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
     8041 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
     8101 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
     8161 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
     8221 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
     8281 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
     8341 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
     8401 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
     8461 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
     8521 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
     8581 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
     8641 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccaa tctacgatgc
     8701 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
     8761 aagtcatctt cgaagtaatc ggattaggat tagttggagg atacgggatg agcttatcaa
     8821 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
     8881 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
     8941 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
     9001 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
     9061 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
     9121 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
     9181 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
     9241 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
     9301 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
     9361 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
     9421 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
     9481 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
     9541 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
     9601 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
     9661 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
     9721 tacttgcaca tgtcgatcga atccgagata atacctacgc ttatctgatt gtagatgatt
     9781 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
     9841 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
     9901 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
     9961 tacaaccgcc agtcaaactt tacgttatac atgcaaataa tttagaagta gcgattgcgg
    10021 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
    10081 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
    10141 tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact ttctacatgt
    10201 tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaacg aagctctttt
    10261 tctttgcaat ccacacttca atcctctata atgtcgcata tagtttagta aatgagtaaa
    10321 ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa tggaaatacc
    10381 aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag ttgatggaag
    10441 aaatgcatta aatacggacg caaaagggct ttctctgggc cttgctcagt ggaatgatcg
    10501 tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat ggtcacgaca
    10561 gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg cgcaaggaag
    10621 tttatacttc caagatgctt atcgctatga aaaattttat gatccagaaa atccagtcgt
    10681 agacatcatt ggattgcaag ggaaccgaat gacggtagaa gtagatacca aaaatccaaa
    10741 gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag aactgattgg
    10801 ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat agacagagat
    10861 gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg aaccttttca
    10921 cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa
    10981 tggcggatcg cctccgaaat taaagggaat tggcaagatc ctgaaatgaa agtcagtaac
    11041 gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct
    11101 gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt
    11161 ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga attactagaa
    11221 gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc
    11281 attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat
    11341 tatccagatg caatccatta cagctattat ccttctgaaa tcgttcgagc gattgaaaaa
    11401 ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca
    11461 cttaactcag cgcttgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc
    11521 catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgtccatta
    11581 aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa
    11641 gtcatggcag aacgtgcgat ggcgaaaacc atgatccaaa aacaagaact tctcttaaag
    11701 atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta
    11761 gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca agatttactc
    11821 cagtcgattt atccaaaagt tttgtataaa ttatccactg atccagatga attggcaatt
    11881 cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa
    11941 gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag cgccgaagaa
    12001 gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa
    12061 tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac aatagaaaaa
    12121 aacgagtttc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg aaaaaatcaa
    12181 aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat ggaagcacat
    12241 gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa ggaaacgatt
    12301 catgaaaaag aaggtctcat cgttcaccga ccatctttcc aaggaaaaac gccggaagta
    12361 cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccgaca aattttagag
    12421 ttactggata atgaac
//
LOCUS       DAHEYX010000069        12364 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHEYX010000069 DAHEYX010000000
VERSION     DAHEYX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.070"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            234..2252
                     /locus_tag="MB219_001346"
     CDS             234..2252
                     /locus_tag="MB219_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCC8427070.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2322..2528
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2679..5093
                     /locus_tag="MB219_001347"
     CDS             2679..5093
                     /locus_tag="MB219_001347"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCC8427071.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5441..6994
                     /locus_tag="MB219_001348"
     CDS             5441..6994
                     /locus_tag="MB219_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCC8427072.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7375..9159)
                     /locus_tag="MB219_001349"
     CDS             complement(7375..9159)
                     /locus_tag="MB219_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427073.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(9174..10895)
                     /locus_tag="MB219_001350"
     CDS             complement(9174..10895)
                     /locus_tag="MB219_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427074.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10995..11756
                     /locus_tag="MB219_001351"
     CDS             10995..11756
                     /locus_tag="MB219_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCC8427075.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4077 a         2208 c         2480 g         3599 t
ORIGIN      
        1 gacatttttt ggtaatgcaa atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc
       61 cttttaaaca agaacgtaga cgtaggaaga acttgaaaat gaacagaagt tgttcattac
      121 ggtaaattac aatatgatta caaaaaacaa caaagctctt aaataacttt taataaatat
      181 taagagaagt tataattatt aataatagat aaattgaagt tggaggatag aagatggaaa
      241 acattgcaag aaaagaacgg agacgtttag aacagaccaa acgttttcga aaagcaaaac
      301 gaggggcagc aatcgtggga actgcgatgg taggctgttc tgtcgctgcc ccattagtac
      361 agccggttcc tgttcaagcg gatgaaacac ctgttcagtt cggcgcaaga atcaatacag
      421 cggcatttat cacagaaatt gctacttatg ctcaaccaat cgctcaagcc aacgatctat
      481 atgcatcagt gatgatcgca caagcagttg tagaaagcgg ttggggcagt agtgcgttat
      541 ctcaagcacc ttattataat ctttttggga taaaaggcag ctatcaagga caaaccgtct
      601 acatggatac gcttgaattt ttaaataatc aatgggtaaa taaaaaggag cctttccgac
      661 aatatccttc ttttgctgaa tctttcaatg acaatgccta tgtacttaga aatacttcat
      721 ttggcaacgg ttattactat gccggtactt ggaagagcaa cacgaaatcc tatacagatg
      781 cgacagcttg tttgacaggg cgttatgcga ctgatcctgg ttacgcaggc aaattaaata
      841 atatcatcgc aacttacgga ttgacccaat atgatacacc agctagcgga aacgcaggcg
      901 gtggagcgac tacaggtggc aataatactg gaagtgcagg tagtactggc ggttcaagca
      961 attctggtaa tacaaactca ggttcagcaa cttcgaccac ttatactgtc caatctggtg
     1021 attctgtttg gggaatctca aataaattcg gtatcacaat ggatcaattg atccaatgga
     1081 acaacatcca aaacaatttc atttatccag gacaaaaact gacaatcaaa ggtagccagg
     1141 aaaatggctc ttcgacgaat aattccggta acaataccaa ctcttcagga aatgctggta
     1201 catcaaacgg aggacaaaca actggtgcga aatacactgt tcaatctggt gattctgttt
     1261 ggaagatctc aaatgatcac ggaattacga tggctcaatt gattgaatgg aacaatatca
     1321 agaacaattt cgtttatcca ggacaacagc taatcgtatc aaatggcggt tcttctaatg
     1381 ctggagcaaa tactggctca aatactggga atacaagtgg ctctagttcg tccagctcaa
     1441 gttcttctaa tacagcttca ggaacgaaat atacagttaa agccggagac tctgtttgga
     1501 gcgtctcaaa taaatacgga attacgatgg ctcagctaat ccaatggaac aatatccaaa
     1561 ataactttat ctacccagga caacaactga ctgtttcaaa cagtggatca acaaacagca
     1621 cgtcaggtaa tacaggttcg tcaaccaata caaataacag cagttcagtt aatactggca
     1681 gcagttcgtc tcaaggcact tataccgtga aagcaggtga atctgtttgg agcgtggcaa
     1741 ataaaaacgg catcacgatg gatcagttaa tcgaatggaa taatatcaaa aacaacttca
     1801 tttatccagg acagcaattg atcgttaaag gtggaacttc ggcaaataca aatacaggtt
     1861 caactacttc tgcagcgaaa ccaaatacac cgaatacgtc tgctacgaca agtacatcat
     1921 ccactggtaa tacgatgtat accgtgaaag cgggcgagtc agtttggagc gtagcgaaca
     1981 aacatcatat cacgatggat cagttaatcg aatggaataa tatcaaaaat aatttcatct
     2041 atccaggaca gcaagtgatt gtgaagaaag gttcatctca atcaacaaac caacaatcag
     2101 ttactggttc gaaaacctat actgtgaaat ctggagaatc tgtttggagt gttgctgaca
     2161 gtcatggaat tacaatggct caactaatcg aatggaacaa tattaagaac aactttattt
     2221 atccaggtca aacgttgatt gtagctaaat aaaatagctg ttcgaagaaa taacgattga
     2281 attccacaaa aatatttgtt actataaggc tacttaaata tgacaacact tgaaaaggaa
     2341 gtagtaaaaa gactgaagtt tcagagagcg catggctggt gagaatgtgc acggatgctt
     2401 ttgaactcgc ctttgagttg cccttgctga atacccgagt aagcaagcgg cggtgacggc
     2461 cgttacaacg ttcgaggcct tttcactaag gtaaaattag gtggtaccgc gtagattacg
     2521 tcctataaaa agagcaatct ttttatagga tttttttatt tcttttgtga agtttgagaa
     2581 aacaaagcaa aaggaaataa aaataaacag attctgccgg ttttttcgtt atatttataa
     2641 tagaaacaag tgttttcaat agaacaggag gacagcaaat gagttacaat cataaagaaa
     2701 ttgagaaaaa atggcaaaaa tactgggcaa aaacgaatac tttcaacacc catgatgatc
     2761 ctgaaaagcc aaaattttac gcactggata tgtttcccta tccatccggt caagggctac
     2821 atgtagggca tcctgaaggt tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc
     2881 aaggatacaa cgtgctgcat ccaatggggt gggatgcatt cggtctgcct gctgaacaat
     2941 atgcgctaga tacaggaaac gatccagctg agtttaccca aaaaaatatc gaaacattcc
     3001 gacgccaaat caattcttta ggatttagtt atgactggaa tcgcgaagtg aatacaactg
     3061 atccggagta ttataaatgg acacaatgga tctttacgaa gctttatgaa aaaggtctag
     3121 cttatgaagc agaagtagcg gtaaactggg tacctgaatt aggaacagtt atttcaaatg
     3181 aagaagtgat cgatgggaaa agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc
     3241 gccaatggat gctgaaaatc actgcttatg ctgatcgctt attggatgat ctagaattag
     3301 ttgattggcc agaaagcatt aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag
     3361 caaatattga atttaaagtg gctggtacgg ataaatccta taccgtattt acgacacgac
     3421 cagatactct attcggtgca acttactcgg ttcttgcacc tgaactagat cttgttagag
     3481 aaattacgac ccctgaacaa aaagctgctg tagaagctta tatcgaagag acagctaaaa
     3541 aatcagatct aaagagaacg gatcttgcaa aagaaaaaac aggagtattt actggtgctt
     3601 atgcaatcaa tccagttaat ggcaaggaaa tcccaatctg gatcgccgat tatgtattat
     3661 cttcatacgg cacaggagcc atcatggctg ttccagcaca tgatgaacgt gactttgaat
     3721 tcgctcaaac tttcggacta gaaattcttc cagttatcga aggtggagat gttgaaaaag
     3781 ctgcttacac agaagatggt acgcacatca actctgaatt tctagacggc atgaacaaac
     3841 aagaagcaat cgataaaatg aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag
     3901 tcagctatcg cttacgtgac tggctattct cccgtcaacg ttactggggt gaaccaatcc
     3961 caatcatcca ttgggaggac ggaacagtca cagctgtccc agaagaagag ctaccattac
     4021 ggttgcctaa gacaaaaaac atcaagccaa gcggtactgg agaatctcca ttagcaaata
     4081 tcgaggagtg ggtcaatgtc gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga
     4141 atacaatgcc acaatgggca ggaagctcat ggtattacct gcgttatatc gaccctcata
     4201 acaaaaaaga actagcagat tatgaaaagt tgaagcgttg gctaccagtt gacatctaca
     4261 ttggtggagc agaacatgcg gtacttcatt tactatatgc acgattctgg cataaattct
     4321 tatacgatat cggtgttgtt cctacaaaag aaccattcca aaaactatac aatcaaggaa
     4381 tgatcttagg cgaaaacaac gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg
     4441 atgatgtggt cgaaacttac ggtgcagata cactgcgcat gtacgaaatg ttcatggggc
     4501 cattagatgc ttcgattgct tggagcgaaa atggcttaga aggaagccgt aaattcttgg
     4561 atcgtgtatg gcgtttgatc gttgatgaga acaataaaat gcgtgaccgt atcactacat
     4621 tgaatgatgg caagcttgat aaagtgtatc atcaaacagt gaaaaaagtg acagaagact
     4681 atgaaaatct tcatttcaat acagcaatct cccaattgat ggtctttatc aatgaagcct
     4741 ataaagtgga cgctctgcct tatgaatata tcgaaggatt tgttcaacta ctcgcaccga
     4801 ttgctcctca catgggtgaa gaactatggg caatccttgg aaatgatggt ggtatttctt
     4861 acgcaccatg gccaacatac gatgaagcag cacttgttga agacgaagta gaagttgttt
     4921 tccaagtaaa cggtaaagtt cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg
     4981 agctagaaaa agcagcatta gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag
     5041 tcagaaaagt tattgcggta ccaaacaagt tagtcaatat cgttgctaat taattggctc
     5101 tttgtcaata aggactgatg agttgtgcaa aatcaaatct gggccagaat gaacttcatt
     5161 ctgtctcaga ttttttgtta tttcaaattg attaattgat tgatcaaaaa ggttttgatt
     5221 ttcatgttct caaacacgtt caatgtcttt ttcttctgaa tggtcttgta agagatctct
     5281 gtaatcatca aatttttgtc tattaatctt agtatttttt gatgtataaa ctacctgaaa
     5341 ttgagagtac ataccaaatt cataaatttt ttattagtgg gactagtgat tgtatggatt
     5401 ttgacgcata atgaatgaag tatgagaaaa ggagagacaa atggatcaag aactatttat
     5461 tatcgaagta ttatcagcta aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag
     5521 aataatgttc aagcttttaa gcttagtatt tttctctggt ttgctttttt tcggagctgt
     5581 atcgttccta tatcgccata tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt
     5641 aaataatttg tatcactttt cgtggggagt tggcggaata acaattgttg tgcttattgt
     5701 ttttcttcta cttatttggc tccgttacca tcaattgtcg cgtcatttcg atcaatggct
     5761 tagtcaaaga gcacaacagt ttttacataa taaaccaact tctcaagatg atgactatct
     5821 ttattttgag aagacttcga cgagtcaagc ttttcggata caaaaaagtg attgccagtt
     5881 attgctgaga tcacttgatg atttacctat ctacttaggc gaaagcaaat caaaattcgg
     5941 aatgaacaca ttccatattt ttttactaga aaacgaaccc agatgggaaa aaagccagtc
     6001 ttcgcctggt ggatacctga caactaagat gagtacatta gtcactggtt ttttcctcat
     6061 tcttttagct ggaacaatct attatgggag atacactcag ccaaaaatgt cttctcatat
     6121 aaatgctgct tctcacatga caatgaactc aactaacgat acgcatatac aaaagacaaa
     6181 tgaagggttg ttagctgatc ctaagcaaaa ggtaaatttg acaacacaaa agataaatga
     6241 tctcaaactt gatacagaga cgcactctct ctatatgaca acagatagtg gtgctaattg
     6301 ggtatttgtg ccgattgatt cgcaatggtt gcgttttggc gaatacaccc tgacaacggg
     6361 gctgattcct gaaggttatt ggatggacaa cacatatgat atttccgccg atttctcatg
     6421 gtttatctat tcttctgggg atgatgtacg tctgctgact tcaagagata atggtaaaac
     6481 atggcaaacg tctattatca cccaatcagt tcagccgata cgttatcgaa aagcggtatt
     6541 ttatggcaac gatggtctat ttatctgtac atcagccagt gggatgtctg cagaaacttt
     6601 gtacatttac actactatgg atggcggaat cacttggata aaaagtggaa gtacaatgat
     6661 caatcagcca gtacaaaatg tcagcttcgt tacaacttct ttaggttttg tttcgacacg
     6721 tgaaagtttg ttttatacaa acaacggagg acgctcattc aaagaatcag ttctgtcgat
     6781 tccggaaaac tatcagacag gggggataga tctttttcaa tcaccaaatg aagttactca
     6841 aataagcgcg aatacgttag agacaaaatt ttatttgttg aaaaaaggac caatcgatgc
     6901 agggaaaatg tttgcctgcc tcttccgttc aactgataac ggggaaacat ggcaattcgt
     6961 tcagcaacta tcgcaagtag aacaaggaga ttaaagaaga gagattgtga gcctgacgtt
     7021 cagcaccgag tcacaagaaa caaattcaaa aaacagcttc tcatatttgt tgaatagtgt
     7081 aactcgtgat tcggagttga caagagcaac aagaagcgat tttgaagtat agcttctcgt
     7141 attttacaag atcgtgtctt attgccgata ggttgtctct tgaacaccgt ttattcgtaa
     7201 aaagggattg tgacaagtct tttgtcacta cttaccgaac gaaactgaag gagctatcca
     7261 aaaagtatca ttagtattat tctttgtgtt tctagtggca ccactagtac atttaaaaaa
     7321 gctatcgatc atggactaat catacatgat cgatagcttt ttagataatg tttgttacgc
     7381 tatctgaaat tgactattat aaagactggc ataaagcgtt ggtgattgga gtagttcaga
     7441 atgtgttcct ttttcgacga tatcgccatt cttcattaca aggatcatgt ctgcattttc
     7501 aatcgttgac aaacggtgtg caatcacaaa acttgtccta tcttttgtca cagcttccat
     7561 tgcttgctga atcaatgctt ctgttcgtgt atctacgcta gaagttgctt catctaaaat
     7621 aacgacagga ggattcgcta gaatgactcg agcaattgtc aataattgtt gctgtccttg
     7681 agataacgtg ccgttttcgc tagaaatgat cgtatcatat ccgtctggca gtgttcgaat
     7741 aaagtgatca cattgtgcga tttcagccgc ttcgataatt tgttctcttg tggcatccat
     7801 tttcccgtag gcgatattat cagctaccgt tccttcaaac agccaggtat tttgtaaaac
     7861 catcccaaaa gaacgacgta gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa
     7921 ttcgatcttt cctttattca gttcataaaa acgcatcagc agattgacta aagtcgtttt
     7981 tcctgcacca gttggaccaa cgatcgctac cgtttgtttt ggctttactg aaaaatcgac
     8041 ttgattcatc aatattttat ctgatgtgta accaaactgt acatttttga aagctatttt
     8101 tcctttcggt tgttctaaaa gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc
     8161 taatatctca aatatcctat cgattgctgc cattgcggat tggatcgagt tgatcacata
     8221 agaagcggta gaaattggtt cagaaatctg attgatgtac tgaagataag cttgtaagaa
     8281 gccgatcgtc acaccacctg atagaacaag catagcaccg aataccgcac ttatgatgaa
     8341 ggcgagttga ttaatgaaac gaatggccgg atagatagca cagttcataa attgagcacg
     8401 tttaaaagcg ttgtattgct ttttatttac ttcgtgtata gcagcttctg catgagcttg
     8461 ctgattgaat gttttgacca ctagattacc agctaagatt tcttccattc gattatttaa
     8521 aacacccagt tcatactgat tctgttcaga aatctgtttg tttttgttcg caatcttagt
     8581 tgtaacaatt gaactgccaa cgatcagcaa taagacgagc aaagtaagct tgctttcgat
     8641 ataaaacagc atcagaccag cagcagctat tgttacgaca gaagtaaaaa attgattgat
     8701 acctgtcaat aacacttgag aaagttgatt gagaccggta gttgtccgac taatgatgtc
     8761 acctacttga tgattgtcaa aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc
     8821 ttttcgcacc ctcaatgtga tcttttcgct taacgctgcc attgtccgtt cctgaataaa
     8881 tgaagtgatg ctgcttaaga ctgaacagcc gatcagcaat aagacaggga acaacaatgc
     8941 ttgtttgata tcactgaaag aaagctgtcc aatacctgtt tttcttattt ctgataagag
     9001 atcatcgatc ccgattccca tgatcaaggg catggctacg actaataggt tcccgaataa
     9061 actgcataga aacaatccgt aaaaatattt atgttcaggt ttgatcattt tccaaaaacg
     9121 tatcaaagca cgcgtgttca acgttttttt cttttccatc gtatcccttc tttctatatt
     9181 ccttgtgatt ttgcaaactc ctgataagtt tggcaattag acagtagttc ttcatgagta
     9241 ccttgtccaa cgatttttcc ttcatctaaa acaatgatgt tatctgcttg ttggattgta
     9301 ctgaggcgtt gagcaacaat cacgagagtt ttctccgaca tgtgtttatg aagggctgct
     9361 cttaatgctg catctgtttt atagtctaaa gcggagaaac tatcatcgaa tacatagatc
     9421 tctgctggtt tgatcaacgc tcgagcaata catatccgct gtttctggcc ccctgaataa
     9481 ttagaaccgc cttgagcaac aaagctgtcc aatccgtcag gaagggtgtc cactagttct
     9541 tctaattgag cgatcttgat cgctttagac aactcttctg ttgaggcata ttcatttccc
     9601 atctgcaagt tgcttaaaat tgtcccgctg aataagaaag ccttctgagg gacataactg
     9661 atgtgggagc ggatctgttc ttgagtcatt tgagtaagag gaataccacc aagggttact
     9721 tttcctatcg tagggtcact aagtctcaag agaagtttcg ccaaactact ttttccagat
     9781 ccggtcccgc caacgatcgc tgttgttttt cctttaggta ttgtgaaatc aatatcttct
     9841 agaactggat cagagatttc agtatattta aatgtgacat gttcaaaagc agctgcattt
     9901 gtctgatcaa aggttggttc agctactgtt ttgatcgtct cttcctcaat ttcgatctct
     9961 gcttgcagta tttcttctat ccgtcgtaat gaggcaaacg atttagggat aatgaccaat
    10021 accatagctg cgatcatcaa gtaagcaagt gtcagcatag agtattcgac tacagcagtc
    10081 acgacaccga tttgtaatgc tccttctgct acaagatttc cgccaaacca caaaatagaa
    10141 gcataaacga ttcccatgag caaaaaagca attggagtga cccaagcaaa tattcggttc
    10201 actttgatca tttgattagc atagtcagca aagctcttat tcgttcgctt ttcttcatat
    10261 gattgattat caaaggcacg gatcatatta atcccagtaa agaattctct caatgtgatg
    10321 gtgatccgat ccatttttgg ctggatactc aaagataaag gtgcccctcg tttcatcagt
    10381 cgataaacaa tccatacata aaaaacaaca gagatcaatg taacccatgt tagtaataga
    10441 gaataacgaa acatcatgag aatggtgaaa acgcaaataa ccggtgctgg taagatcatt
    10501 tggcaaaata aaacaatcat ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa
    10561 agagatccag aactaaaacg actgatatcc ttcattgaca gctgttggaa tttacggaaa
    10621 tatttctccc gcgtttggaa tccaaaccta gcggaagcca atgctgaaaa gtaactagag
    10681 gcaactgcag cgattgttcc tagaaacgct acaagcagca tttcaactcc aatttttttt
    10741 ataactgagg cttcgccact tgctatacct gtgtcgatca gctttgctac taattttggt
    10801 acacctaatg taccggcgat ttctaaacag atcatcacaa aggtaaggag agcgagtcct
    10861 ttgtttttct gtaagaatgt ttttaagata gacatattat tcctcctttt ttcctgatac
    10921 ttttcgattc tataccttca agtaacttga aggtcaatag aaaaaagaat aaactgaaaa
    10981 agaggtgaga aaaaatgaaa aaaatgtata cgattggcca agtatcggaa ctatttgatt
    11041 tgccaaaatc aacgatccgc tattgggatg aacaaggatt aatccgttca agtagacaag
    11101 aagagaatga ttatcgattg tttgatatcg atgatatttt tatgctttat gatatcgttt
    11161 tttatagaaa attagatata ccgatcaaac agatgaagaa tttatatgga aaaacattga
    11221 cggagttata tgagacactt gatgaaacag aaagaagaat ccataaagaa ctggtggtga
    11281 tgaaacaaaa acaaaaagag ataagggagc aaaaaaaaca gttgaaattg atgatcgata
    11341 caaacgagga agaatttcct gtagaagaga tcccttttga ctgtatgatc tcaacggagt
    11401 ttgaagatat tgtggaaatc aaaaaatttc tacctaacta ttcaagcttt gggatgatga
    11461 gtatgccagc ttccagttca cagacgatgt atggattttt tatcgaccct agtgaagtac
    11521 atttatttac tcaagacgtg atttgggaga aaaaagatac ggctgtttat cgtcgattct
    11581 tattgaaatc cgagatgaac catgcggaac gtaataatat tttagaaatc agagaaagaa
    11641 tgtatgaaaa aggatggaaa acaggagaag tgatcggcca gtatctattg acgaatacgg
    11701 atgaaaacaa tatcagaact gaatattatc atgcatggat agaaatgaaa aaataggaaa
    11761 tatgtgttta agataactcg aaaatgatgg ttgttttagt tataaagaaa aattttctgg
    11821 ttcgattacg ggtttaatgt gggggattat tagtcgtgga tagatagaat tttagttttt
    11881 tagaggaaat atgtctttaa aaatggaatg gttgcaattc ttagcgatat atacgatgta
    11941 attgtcctct taaaaaagta tggaaaaata attgtattgg gtgttatgta taattcatgg
    12001 tgaactgaga ctaatgagaa gaaaaattca tattgttatg agtgagattg tatcagatga
    12061 gaatgataaa ttaaataggt gcataaaagc attacactta aaatacattg ttgcttacat
    12121 tattacaata tgttaataag gcaaaaataa atagatggat tgaataaaca tattttttaa
    12181 tcaaatagtc cctactgtga ctttcctcag ctatcggtga tactaccgat agcatttttt
    12241 tgcttataaa ataattataa gtatttagag atttttcttc taacattctt caaacaagaa
    12301 aaatcacaaa ggttattgtt atttttatat attcttttta agaatgattg tttttttata
    12361 gaaa
//
LOCUS       DAHEYX010000070        12171 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHEYX010000070 DAHEYX010000000
VERSION     DAHEYX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.071"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(133..297)
                     /locus_tag="MB219_001352"
     CDS             complement(133..297)
                     /locus_tag="MB219_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427076.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="MB219_001353"
     CDS             592..1920
                     /locus_tag="MB219_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCC8427077.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="MB219_001354"
     CDS             2122..2955
                     /locus_tag="MB219_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCC8427078.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="MB219_001355"
     CDS             2971..3708
                     /locus_tag="MB219_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCC8427079.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="MB219_001356"
     CDS             complement(3863..4831)
                     /locus_tag="MB219_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCC8427080.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="MB219_001357"
     CDS             5063..5896
                     /locus_tag="MB219_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCC8427081.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="MB219_001358"
     CDS             5930..6769
                     /locus_tag="MB219_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8427082.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="MB219_001359"
     CDS             6795..7355
                     /locus_tag="MB219_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCC8427083.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="MB219_001360"
     CDS             7554..9275
                     /locus_tag="MB219_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCC8427084.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="MB219_001361"
     CDS             9440..10582
                     /locus_tag="MB219_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCC8427085.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="MB219_001362"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="MB219_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCC8427086.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAHEYX010000071        12040 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHEYX010000071 DAHEYX010000000
VERSION     DAHEYX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.072"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            180..2345
                     /locus_tag="MB219_001363"
     CDS             180..2345
                     /locus_tag="MB219_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8427087.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            2649..3482
                     /locus_tag="MB219_001364"
     CDS             2649..3482
                     /locus_tag="MB219_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCC8427088.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(3584..5284)
                     /locus_tag="MB219_001365"
     CDS             complement(3584..5284)
                     /locus_tag="MB219_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427089.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            5791..6951
                     /locus_tag="MB219_001366"
     CDS             5791..6951
                     /locus_tag="MB219_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCC8427090.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6967..7410
                     /locus_tag="MB219_001367"
     CDS             6967..7410
                     /locus_tag="MB219_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427091.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(7893..8423)
                     /locus_tag="MB219_001368"
     CDS             complement(7893..8423)
                     /locus_tag="MB219_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCC8427092.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            8528..9079
                     /locus_tag="MB219_001369"
     CDS             8528..9079
                     /locus_tag="MB219_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCC8427093.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(9232..9705)
                     /locus_tag="MB219_001370"
     CDS             complement(9232..9705)
                     /locus_tag="MB219_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCC8427094.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            9843..11315
                     /locus_tag="MB219_001371"
     CDS             9843..11315
                     /locus_tag="MB219_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCC8427095.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            11312..12022
                     /locus_tag="MB219_001372"
     CDS             11312..12022
                     /locus_tag="MB219_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCC8427096.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         3962 a         2055 c         2470 g         3553 t
ORIGIN      
        1 ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa gggaacacac
       61 gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc atttacaaaa
      121 aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag ctaaacggaa
      181 tgaaaaagat atttagtttt gaattttggc aaaaatttgg aaaggcttta atggttgtgg
      241 tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt cccttgatca
      301 atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt ggttgggcaa
      361 ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg gcaaaagaaa
      421 gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg atcactgggg
      481 ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc cacactctgt
      541 tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca ccagctttga
      601 atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct tataataaat
      661 actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag cgctttgttc
      721 cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg gtgtggccaa
      781 acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa gaaagcgcac
      841 cgattctagc accgttttta tttggaacat tagaacgttt actattgcca tttggattgc
      901 atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat gagattttat
      961 caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg gcttgggcca
     1021 ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt gtgttgcata
     1081 actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt atcttgatgg
     1141 gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga tataaatcaa
     1201 tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca ttggagttca
     1261 tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa ggggctgcat
     1321 ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc gaattgctga
     1381 ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac ttcgtgttgt
     1441 gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc aaaaaattca
     1501 actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa accgttgtat
     1561 ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat ttgctgggag
     1621 gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta agtgtaaatg
     1681 atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg ggattgatcg
     1741 tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg aaatcagata
     1801 tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt tctacaggaa
     1861 atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt tccgcagctt
     1921 ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa aaaatgatgg
     1981 gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc gctggaaaag
     2041 tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat ggtatcgaag
     2101 ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt gagattgctg
     2161 tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat cttgatgtga
     2221 tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat aaagtagaag
     2281 aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc tccattaaaa
     2341 tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag ccaaacaaaa
     2401 atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca gtttgaatac
     2461 tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa cagccaaaca
     2521 aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga acactatttg
     2581 ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg aagacaagga
     2641 gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca gaaccagaga
     2701 aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg attgctttac
     2761 aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta atgaagttct
     2821 gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt ttagttcagc
     2881 ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac atcggttatg
     2941 ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt caggtcctta
     3001 ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg attgggaaaa
     3061 caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg tggacagata
     3121 aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg gaaactcgcg
     3181 taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt tattatactg
     3241 taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa aaaggaaaag
     3301 cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa ttgcgtatag
     3361 attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt tttgatggga
     3421 tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat gtgaacgaat
     3481 aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca aacgattata
     3541 ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca cgtaaaagct
     3601 tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa gtaagagaaa
     3661 gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg attccttttt
     3721 gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat gtttttccat
     3781 tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt ttgtccccag
     3841 tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa gttgtttcgt
     3901 tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc ttatagattg
     3961 gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata tctttgctga
     4021 agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga ctgacaaatg
     4081 tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt gctgaaaaaa
     4141 taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac tggttcagtg
     4201 aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga aatggaaact
     4261 ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga atcaagctag
     4321 tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat aaggtgttct
     4381 tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga aaggccttag
     4441 ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt tctctgttaa
     4501 caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc caaatatttg
     4561 ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct tttccttttt
     4621 cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct aacatgatcg
     4681 aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg ataacaggga
     4741 actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa aaacaagtcc
     4801 cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta ctcatgaata
     4861 aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt aaaatcagca
     4921 tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa tgaaaccagc
     4981 tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg taaacggcat
     5041 tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg aaatccccat
     5101 atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca tcagcaccca
     5161 aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg aaaaaaattg
     5221 ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta gataaattca
     5281 tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ctcatgaaaa tatttatgaa
     5341 ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat aagttttgtt
     5401 gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta gctttttcat
     5461 gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca ctagcctaga
     5521 aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa cgcagttttc
     5581 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaacccaa
     5641 caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca ggacagcttt
     5701 ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata ataaaagaaa
     5761 gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa acagtctcgt
     5821 cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact gatcgcttta
     5881 tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat cgaacaagta
     5941 caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga gaaaaaccaa
     6001 cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa gaacagaaat
     6061 gaaagtacaa catccagtca attagaggaa tggctgatcg gaagtatcaa tatacctaag
     6121 atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga aaatggagca
     6181 ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc agtgatctct
     6241 gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt agaaaacgga
     6301 gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa aaatatacaa
     6361 gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga cttagttacc
     6421 ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac aggacatcgg
     6481 attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga aagaaccatg
     6541 cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt agtgtttatc
     6601 ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc atttattatt
     6661 agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca taagaaaaaa
     6721 acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg gaaagtcaag
     6781 ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc taaagttgct
     6841 gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga ggggcgaaaa
     6901 cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata agtaaggaga
     6961 gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc tgttctatcg
     7021 atttggctgc atgctaactt ggattctcat gaattcattc catcaactta ttgggagaat
     7081 caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc agaaatagat
     7141 caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat ctttgtgaaa
     7201 agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa agaaaccaat
     7261 aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt ctatctttca
     7321 caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga ggaacttgtg
     7381 atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa tgctcccagc
     7441 ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact gtgtgaatat
     7501 gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7561 aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc aggtcaaaac
     7621 gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg taataagccc
     7681 actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac tcaaagcata
     7741 acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc ggtattagct
     7801 caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata ctcaggtcag
     7861 aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata attttcatcg
     7921 aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc gctaataaca
     7981 tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc aggaacaagt
     8041 aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat atgttcgttt
     8101 tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa gatcattccg
     8161 tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt tggctctgtc
     8221 caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg gtcttttacc
     8281 caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc atttttggca
     8341 taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc aataaaatct
     8401 tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa catgtaataa
     8461 actacatgtt ttcgattact catttatgat agaatggatt taaagatttg tcaaggaggg
     8521 aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat taagtaagaa
     8581 gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag aattttggca
     8641 agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag gaatcattca
     8701 gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga
     8761 agggaaggaa caattggcag agtggattca tacacccacc aatgaactac cggttaatcg
     8821 agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg cattagtaga
     8881 aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat tagagcgaaa
     8941 aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact acctgatttt
     9001 agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg tattagaaga
     9061 aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct ttctatatga
     9121 aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt ttcaagtatt
     9181 tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg tttagagttc
     9241 ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt aagttaattc
     9301 gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga agttaccgat
     9361 ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac
     9421 tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag cttttgacaa
     9481 agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg taggtaaacg
     9541 atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct tttgttccca
     9601 ataccttaat tgacgtggag acacacctac gatatcactt aattcactga tgccaacaac
     9661 caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg tcactccttt
     9721 tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta ggttgacaaa
     9781 tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa agaaggagaa
     9841 atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg acacttttag
     9901 ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta ttgacgacag
     9961 cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa tggttaacga
    10021 ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg atcaataaat
    10081 tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga acgattactt
    10141 gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa gcttgcggtg
    10201 tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa
    10261 gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct ttaggcccta
    10321 cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt atggtactgc
    10381 caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt attccattgt
    10441 caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc ggtagtttac
    10501 tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta attggtttct
    10561 tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat ttggaacatc
    10621 cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct gttttagctg
    10681 gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat attcaaaata
    10741 ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca ttgatcatgg
    10801 gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca
    10861 tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg acaaaaacga
    10921 ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc tccatggtca
    10981 tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc agtcatggga
    11041 cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct attttgatca
    11101 gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg gataatacgc
    11161 cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat gccgcgttta
    11221 tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg aagaaaaaag
    11281 aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga ttgtcagctt
    11341 gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt
    11401 gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt tgtccaatca
    11461 ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca gtgctgattc
    11521 ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa aagtgtatct
    11581 ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata acgaaacaaa
    11641 taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt attgggaata
    11701 caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg aatatgacag
    11761 ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg accaagcagc
    11821 caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag caaaagctta
    11881 tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca aagcagagca
    11941 agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg caaaattagt
    12001 gaaagaagca aaaggcgaat aatattttga acaccatgta
//
LOCUS       DAHEYX010000072        12036 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHEYX010000072 DAHEYX010000000
VERSION     DAHEYX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.073"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            25..507
                     /locus_tag="MB219_001373"
     CDS             25..507
                     /locus_tag="MB219_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427097.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            704..1594
                     /locus_tag="MB219_001374"
     CDS             704..1594
                     /locus_tag="MB219_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCC8427098.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1881..3155)
                     /locus_tag="MB219_001375"
     CDS             complement(1881..3155)
                     /locus_tag="MB219_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCC8427099.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3428..5899
                     /locus_tag="MB219_001376"
     CDS             3428..5899
                     /locus_tag="MB219_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCC8427100.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6002..7738)
                     /locus_tag="MB219_001377"
     CDS             complement(6002..7738)
                     /locus_tag="MB219_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCC8427101.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7735..8439)
                     /locus_tag="MB219_001378"
     CDS             complement(7735..8439)
                     /locus_tag="MB219_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8427102.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8570..9073
                     /locus_tag="MB219_001379"
     CDS             8570..9073
                     /locus_tag="MB219_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCC8427103.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9033..9371
                     /locus_tag="MB219_001380"
     CDS             9033..9371
                     /locus_tag="MB219_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCC8427104.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9392..10810
                     /gene="ffh"
                     /locus_tag="MB219_001381"
     CDS             9392..10810
                     /gene="ffh"
                     /locus_tag="MB219_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCC8427105.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10922..11500
                     /locus_tag="MB219_001382"
     CDS             10922..11500
                     /locus_tag="MB219_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCC8427106.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11504..12025
                     /locus_tag="MB219_001383"
     CDS             11504..12025
                     /locus_tag="MB219_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCC8427107.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
BASE COUNT         3871 a         2189 c         2528 g         3448 t
ORIGIN      
        1 aaaaagtagt ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta
       61 tttttttggt tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa
      121 ggtgcggaga tattaggcct gatccaagga agcggatttg tggtttctag gaattttcca
      181 gctttgattg ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc
      241 aatcactaca attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt
      301 tctttgctgc ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat
      361 ttagcttatt cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc
      421 tcatttattc ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga
      481 tctgtatcta taaaaaataa ctattgatgg tggatcatca gtaaaaaaac atatgagctg
      541 cgtaaaatgc atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt
      601 ttacatgatt tttacataac ttaactatat ttttacagca accatacgag ggatttacag
      661 tcgactgata aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt
      721 tactctaggt atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga
      781 tggctctgcc acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt
      841 aggttcgaca gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa
      901 tccaaattat caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga
      961 agctggagca gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga
     1021 tgcttcaaaa ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa
     1081 agatacagat gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat
     1141 cactaactgg aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa
     1201 cggcagcggt acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca
     1261 gtcccaagaa caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc
     1321 aatcagctat ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg
     1381 tgtagaacca aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca
     1441 tatgtacacg aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac
     1501 agaagaaatc caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga
     1561 agtagaacgt gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt
     1621 cgttactatt aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattgt
     1681 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga
     1741 acaccgttta ttcgaaaaaa agagctacga catagaactg tcgtaacttt ttttggctct
     1801 tcgtcaaatc gtgttggtga agacaaattt gaataaaact tagtccgata tgcggcgcgt
     1861 tctgtgcgcc gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt
     1921 gcccgaaagt tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg
     1981 ttattcagcc cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc
     2041 gattggtagc aatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa
     2101 attgttttaa gggtttcttg aaagccttcc gtacctcgtt taacgaaata atagcgtaat
     2161 aattgacaaa aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcatcaacg
     2221 atttctgttt gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtggctcc
     2281 catgtcgtgg aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc
     2341 tgattttcag agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag
     2401 ctgcgattga tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag
     2461 actgttttaa gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt
     2521 aaacgggcct ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct
     2581 tcaaggatcg taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca
     2641 gaaacagaat tcattgattt gaactcatcc atacacaaca ccgtcggaag gaagttcata
     2701 cgtggctggt aatgcttcgt tgcttcacgc aagatccgtg tcaccgtccc atccgagacg
     2761 aagtacttct tagcgattag tttacgtgat tgattttcgt acagttccat gagaatcgcc
     2821 tgcttcagtt cctttgttag ttgatgatgt tcatcaacta agtccgtttt cgcagaaaag
     2881 gtccttccac aatcatggca gagaaaacgg gtacgtttta attctaaaat agttaacttt
     2941 tctttgactt tcaacatctg agtgcgtgtc gtataggaac cgttgcggat aatacgaacc
     3001 gatcgacagt gttcacaata ggaagaagag tcatccaatt ttcctttaat aaactgcgct
     3061 tgaacgcctt tatattcttt ttcaatcagc cattcttcat caaaaaataa atgtttatct
     3121 gttaatccga gtaattttct agtatgatta tccattgaga gactcctttg gtaggatgac
     3181 gttttggtcg actaaatttt accaaaagtg agtctctttt tgagttgaaa cgaagaaaaa
     3241 gtgttagtag aaaatcgcct tcgattttcc accaacacaa aatattatag aacccttttt
     3301 ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat
     3361 atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg
     3421 tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc
     3481 atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt
     3541 caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa
     3601 tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc
     3661 cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc
     3721 caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa
     3781 tgttttaaat acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat
     3841 agaagacctt gtaatagatg acattgttat tttagagact ggatatcaaa ttccagcaga
     3901 tgcagaggtc gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga
     3961 tgaaataacc aaagaaatag gtgatccatt aatgtccggc agtttcattg tttccggaaa
     4021 agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa
     4081 agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat
     4141 caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt
     4201 ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat
     4261 gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact
     4321 agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa
     4381 tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaaat
     4441 gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc
     4501 tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt
     4561 tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt
     4621 cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg
     4681 cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt
     4741 gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac
     4801 accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga
     4861 ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt
     4921 atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa
     4981 attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa
     5041 gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac
     5101 aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc
     5161 ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc
     5221 acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc
     5281 cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt
     5341 tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg
     5401 acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat
     5461 catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc
     5521 gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac
     5581 catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa
     5641 attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt
     5701 gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct
     5761 atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga
     5821 acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat
     5881 atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc
     5941 gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt
     6001 tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag
     6061 taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc
     6121 cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct
     6181 gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg
     6241 cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca
     6301 gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa
     6361 tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct
     6421 ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca
     6481 gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa
     6541 gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac
     6601 caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt
     6661 gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg
     6721 taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa
     6781 gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga
     6841 gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta
     6901 aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga
     6961 atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtacacg
     7021 cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac aattcttccc
     7081 attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta
     7141 gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc
     7201 ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag
     7261 ttctcggtaa aaaaacgctt gtcggttctg cgatacgtaa aatggcttct aactggccac
     7321 tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta cttttgcgca
     7381 aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct
     7441 gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag
     7501 taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt
     7561 ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt
     7621 cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa
     7681 acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg
     7741 tttgggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact ttgggtgttt
     7801 tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg
     7861 tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc
     7921 ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg
     7981 gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata
     8041 ttcaggctca gcttcatctt ttgattgctt acgaggttca attcgtctga atattgcttt
     8101 catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat
     8161 ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt
     8221 ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat
     8281 cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc
     8341 atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac
     8401 aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt
     8461 ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata
     8521 aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat
     8581 cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga
     8641 attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg
     8701 attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa
     8761 agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg
     8821 cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga
     8881 aaaaagccct tcatgcggaa cccattcgat ttatgacgga agtttttccg gaataaaaaa
     8941 aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga
     9001 tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga
     9061 acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat
     9121 tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac
     9181 aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac
     9241 tccatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata
     9301 ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa
     9361 tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct
     9421 ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga
     9481 aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa
     9541 agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc
     9601 acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga
     9661 aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca
     9721 aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa
     9781 tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa
     9841 agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt
     9901 agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat
     9961 cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa
    10021 agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc
    10081 cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa
    10141 acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc
    10201 aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg
    10261 tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca
    10321 agactacgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga
    10381 tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct
    10441 aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc
    10501 aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa
    10561 tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt
    10621 tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat
    10681 gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg
    10741 aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa
    10801 acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct
    10861 tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca
    10921 gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg
    10981 gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca
    11041 agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga
    11101 tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga
    11161 aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc
    11221 agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa
    11281 tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct
    11341 ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac
    11401 aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg
    11461 caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt
    11521 ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct
    11581 ttttgacact ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac
    11641 gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat
    11701 ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc
    11761 tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa
    11821 agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat
    11881 tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca
    11941 aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat
    12001 cgaattgcat tatgtacgaa agtagaagta aataaa
//
LOCUS       DAHEYX010000073        12013 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHEYX010000073 DAHEYX010000000
VERSION     DAHEYX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.074"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(56..643)
                     /locus_tag="MB219_001384"
     CDS             complement(56..643)
                     /locus_tag="MB219_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427108.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            818..2140
                     /locus_tag="MB219_001385"
     CDS             818..2140
                     /locus_tag="MB219_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8427109.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2295..3071
                     /locus_tag="MB219_001386"
     CDS             2295..3071
                     /locus_tag="MB219_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCC8427110.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3064..3633
                     /gene="rnmV"
                     /locus_tag="MB219_001387"
     CDS             3064..3633
                     /gene="rnmV"
                     /locus_tag="MB219_001387"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCC8427111.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3626..4510
                     /gene="rsmA"
                     /locus_tag="MB219_001388"
     CDS             3626..4510
                     /gene="rsmA"
                     /locus_tag="MB219_001388"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCC8427112.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4961..5590
                     /locus_tag="MB219_001389"
     CDS             4961..5590
                     /locus_tag="MB219_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427113.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MB219_001390"
     CDS             complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="MB219_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCC8427114.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7032..7454
                     /gene="mgsA"
                     /locus_tag="MB219_001391"
     CDS             7032..7454
                     /gene="mgsA"
                     /locus_tag="MB219_001391"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCC8427115.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7777..8313
                     /locus_tag="MB219_001392"
     CDS             7777..8313
                     /locus_tag="MB219_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCC8427116.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8353..10116)
                     /locus_tag="MB219_001393"
     CDS             complement(8353..10116)
                     /locus_tag="MB219_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427117.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10119..11834)
                     /locus_tag="MB219_001394"
     CDS             complement(10119..11834)
                     /locus_tag="MB219_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427118.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         3752 a         2219 c         2452 g         3590 t
ORIGIN      
        1 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
       61 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      121 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      181 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      241 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      301 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      361 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      421 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      481 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      541 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      601 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      661 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      721 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      781 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      841 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      901 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
      961 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1021 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1081 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1141 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1201 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1261 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1321 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1381 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1441 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1501 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1561 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1621 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1681 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1741 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1801 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1861 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1921 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     1981 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2041 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2101 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2161 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2221 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2281 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2341 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2401 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2461 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2521 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2581 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2641 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2701 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2761 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2821 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2881 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     2941 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3001 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3061 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3121 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3181 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3241 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3301 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3361 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3421 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3481 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3541 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3601 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3661 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3721 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3781 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3841 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3901 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     3961 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4021 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4081 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4141 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4201 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4261 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4321 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4381 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4441 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4501 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4561 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4621 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4681 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4741 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4801 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4861 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4921 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     4981 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5041 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5101 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5161 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5221 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5281 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5341 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5401 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5461 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5521 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5581 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5641 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5701 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5761 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5821 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5881 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     5941 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6001 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6061 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6121 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6181 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6241 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6301 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6361 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6421 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6481 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6541 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6601 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6661 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6721 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6781 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6841 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6901 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     6961 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7021 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7081 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7141 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7201 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7261 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7321 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7381 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7441 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7501 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7561 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7621 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7681 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7741 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7801 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7861 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7921 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     7981 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8041 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8101 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8161 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8221 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8281 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8341 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8401 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8461 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8521 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8581 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8641 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8701 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8761 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8821 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8881 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     8941 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9001 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9061 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9121 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9181 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9241 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9301 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9361 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9421 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9481 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9541 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9601 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9661 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9721 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9781 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9841 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9901 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     9961 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10021 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10081 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10141 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10201 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10261 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10321 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10381 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10441 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10501 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10561 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10621 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10681 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10741 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10801 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10861 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10921 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    10981 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11041 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11101 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11161 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11221 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11281 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11341 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11401 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11461 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11521 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11581 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11641 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11701 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11761 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11821 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11881 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    11941 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    12001 atgagaaaac atg
//
LOCUS       DAHEYX010000074        11943 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHEYX010000074 DAHEYX010000000
VERSION     DAHEYX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.075"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            88..1275
                     /locus_tag="MB219_001395"
     CDS             88..1275
                     /locus_tag="MB219_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCC8427119.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1382..1723)
                     /locus_tag="MB219_001396"
     CDS             complement(1382..1723)
                     /locus_tag="MB219_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427120.1"
                     /translation="MLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKV
                     VELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVKSTILCKS
                     STSSKAQCFAL"
     gene            complement(2159..2518)
                     /gene="rplT"
                     /locus_tag="MB219_001397"
     CDS             complement(2159..2518)
                     /gene="rplT"
                     /locus_tag="MB219_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCC8427121.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(2571..2771)
                     /gene="rpmI"
                     /locus_tag="MB219_001398"
     CDS             complement(2571..2771)
                     /gene="rpmI"
                     /locus_tag="MB219_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCC8427122.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(2843..3367)
                     /gene="infC"
                     /locus_tag="MB219_001399"
     CDS             complement(2843..3367)
                     /gene="infC"
                     /locus_tag="MB219_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCC8427123.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(3411..3536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3687..4319
                     /locus_tag="MB219_001400"
     CDS             3687..4319
                     /locus_tag="MB219_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCC8427124.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(4321..5226)
                     /locus_tag="MB219_001401"
     CDS             complement(4321..5226)
                     /locus_tag="MB219_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCC8427125.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(5311..5970)
                     /locus_tag="MB219_001402"
     CDS             complement(5311..5970)
                     /locus_tag="MB219_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCC8427126.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6286..7230
                     /gene="mvk"
                     /locus_tag="MB219_001403"
     CDS             6286..7230
                     /gene="mvk"
                     /locus_tag="MB219_001403"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCC8427127.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            7223..8200
                     /gene="mvaD"
                     /locus_tag="MB219_001404"
     CDS             7223..8200
                     /gene="mvaD"
                     /locus_tag="MB219_001404"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCC8427128.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            8213..9298
                     /locus_tag="MB219_001405"
     CDS             8213..9298
                     /locus_tag="MB219_001405"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCC8427129.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            9295..10359
                     /locus_tag="MB219_001406"
     CDS             9295..10359
                     /locus_tag="MB219_001406"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCC8427130.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(10608..11411)
                     /locus_tag="MB219_001407"
     CDS             complement(10608..11411)
                     /locus_tag="MB219_001407"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCC8427131.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(11431..11736)
                     /gene="kduI"
                     /locus_tag="MB219_001408"
                     /pseudo
     CDS             complement(11431..11736)
                     /gene="kduI"
                     /locus_tag="MB219_001408"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(11718..>11943)
                     /locus_tag="MB219_001409"
     CDS             complement(11718..>11943)
                     /locus_tag="MB219_001409"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCC8427132.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3565 a         2473 c         2323 g         3582 t
ORIGIN      
        1 taaaagtgaa gcatcaaaaa agccattgat ctcagttagg agtacaatgt tttttgcaga
       61 aaacatactc gaataggact gatatcaatg actcaagtac attttacttt tgaaagcgaa
      121 gaaattcaag caattataaa cgaaagcggt gcgaatgata ccgcaaaaac acttatgact
      181 atcatgttta atcagctgat ggaagaacaa cgaaatcaat atattcaggc aaatgcctat
      241 gaacgttcag aagaacggca atcacaaaga aatggttact acgatcgttc gtttacaact
      301 cgaattggta cgttagaact tcatgttcca cgaaccagag acggtaagtt tagtcctact
      361 atctttgaaa gatatcagcg aagcgaaaaa gctttgatcg ctgccatgat tgaaatggtc
      421 atttctggtg tctccactcg caaagtaact aaaacagttg aactgctaac ggatggtgcg
      481 actgtttcta aatcattcgt ttcaaatctg atgaaacagt tggatccatt tgtttttgag
      541 tggagaaacc ggagcctaga aggctcggaa tatccatttt tcatgtgtga tgccctgtat
      601 atgaaagtaa gggaaaatca tcgtattgtc tcaaaaggtg tatatattgg tatcggcatt
      661 gattctgatg gacgacgtac gattcttggt tttgatgttc aagacggcga atcggaagat
      721 aattgggata ccgtttttca atcgttcgtt caacgaggtt tgttcggtgt aaaactggtc
      781 atttctgatg cacacaaagg gttagttaag gctgttcgca agaacttttt aggtgcgagc
      841 tggcaaagat gccaagccca ctttttgaga aatatttttg ataagctgcc aaagaaagtt
      901 tcttctgatg taaaagacga gttgaagagt atctttaaag cctctgaact tgaattgaca
      961 cgtgaaagaa aagagcactt cttggaaaaa tatggttgtg attcaaaatt aagtgctgct
     1021 tgcgacattt tagaaaacgg ctttgaagat gctattcaaa ttttgtcttt tcctgaaaat
     1081 atccgccgaa ggattcgtac aaccaatgtg ttggaacggt tgaacgaaga gattcgccgg
     1141 agagaacgag tgattcggat ttttccaaat atcaattcca tcactcggat aattggaaca
     1201 cttttgatgg aaaaagacac tgagtggctg gcttctccac gaaaatattt agaatttaat
     1261 tcgaataaca tttagcacca tgaacctgtg cgagtctctt ctttctgagg agagacatca
     1321 agagtcgctg cgctactgct cctgacctct ctcctcagaa agaaaggcat ggcagttagg
     1381 ttcacaacgc aaaacattgt gcttttgatg aggttgaact tttacacaag attgtggact
     1441 tgactgaaat acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg
     1501 gtccgattcc tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg
     1561 tgattcttgg cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc
     1621 cgctatctac tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt
     1681 tggcgtactc agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc
     1741 cttccatcgc cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag
     1801 tagccatgat gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg
     1861 aaaaagaatg agataactaa caaaatacca ataataatat cactcatgtt attcctccgt
     1921 ccattttgtt tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta
     1981 gttagcgctt tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat
     2041 tatgttattt tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa
     2101 aaaaagagga aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt
     2161 atttagccaa agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa
     2221 cagccaagtc agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca
     2281 ttaattttga gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca
     2341 atttacggaa gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca
     2401 ttacttgttc ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta
     2461 attttagcac tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt
     2521 ataattcctc ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt
     2581 tttgctagtt gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc
     2641 aattgacggc gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag
     2701 cgtttcaatc cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt
     2761 tgttttggca tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc
     2821 aacagccagt tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca
     2881 tgctgcgtcc gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag
     2941 ctaagcgatt caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac
     3001 ggatagaagc tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg
     3061 tattgaagtc attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt
     3121 tttgtttttt acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt
     3181 ccatgattcg cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg
     3241 cttgttctgc aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg
     3301 aaccgatcaa tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta
     3361 tggtcattca cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa
     3421 aaaaacgagt tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta
     3481 tctagcccag tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca
     3541 accattatag tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc
     3601 cttttctgaa aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat
     3661 actaaagaaa aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc
     3721 ctcttcttac aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga
     3781 tcaacggagg taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata
     3841 tccaaaaact tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt
     3901 ttgaaaaatg tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt
     3961 ttcatcaatg taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt
     4021 tcactgattg tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct
     4081 tcgatcattg tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt
     4141 tgtctgacaa tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact
     4201 ttacgactaa tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg
     4261 tcaatcaaga gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga
     4321 tcaacgaatc cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat
     4381 caaggcgatt tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa
     4441 gaatcctgtc acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat
     4501 cgtgtcattc aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat
     4561 ctgcgaagct aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa
     4621 ctcttctttt tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa
     4681 aagtactaca ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga
     4741 aagttgcagc agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg
     4801 ttcactattc acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg
     4861 aaacaataaa ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg
     4921 aatcacgtac tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa
     4981 agcgatagga ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac
     5041 aagcagtaac gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag
     5101 agaataagtc agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag
     5161 cacatcgttt tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc
     5221 ccgcatactc tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa
     5281 aggtttaccc tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc
     5341 tgctaatttt tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa
     5401 attatagcgg tctccatcga tttggatttt tggactttct ttgtattctt cataccattg
     5461 atcataacgt ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg
     5521 ttcatacgaa cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat
     5581 atgaactaat aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt
     5641 ttccagcagt tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt
     5701 tagatggaaa agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt
     5761 cattgcttgt ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc
     5821 atactgttca gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata
     5881 aaatgcctga ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc
     5941 aattgtacct gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat
     6001 tgtgtttaac tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt
     6061 ttttctagtc aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt
     6121 agaaaacgta ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt
     6181 ttttgagacc cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt
     6241 tattttttgc ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc
     6301 caaggagaat caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa
     6361 ccagcgattg cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat
     6421 gcaatggacg atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat
     6481 gcattaaaaa atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa
     6541 aacttgcaac tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct
     6601 gcagtcgcca cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt
     6661 gaaatactat tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga
     6721 atcgatgcag ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt
     6781 gactactttt ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga
     6841 caaacaagag aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact
     6901 ggacaaatga tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag
     6961 aattcaccag aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg
     7021 acaatcagca atgattttct taatctattg atccaaacag caaaagatac cggggcattg
     7081 ggtgccaaat taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa
     7141 acaaaagccc aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata
     7201 caaggattag gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc
     7261 tctaatcaaa tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct
     7321 ttcgttaaca ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat
     7381 ggtagatgaa ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag
     7441 tcagtttctt gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag
     7501 tcagaatttc gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt
     7561 agcaggagct tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt
     7621 tgctcgacgc gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga
     7681 aaaaggccat gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga
     7741 cgatttagca atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa
     7801 tgggatgcgt cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga
     7861 aggggatcta tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga
     7921 aacgatggaa agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt
     7981 cacttactgg tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa
     8041 aggcattcca tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa
     8101 cagccattta tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat
     8161 tactgcccat gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga
     8221 agtatctgca ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca
     8281 tccagcagtt atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa
     8341 agtcggaagt atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa
     8401 cggagaatta gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg
     8461 tttgactgaa aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt
     8521 aacaagtgag ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt
     8581 gaccgttgca acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga
     8641 gattttcaag attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga
     8701 catcgctgct agctgttatg gtggctggat cgctttctca accttcgacc atccttggct
     8761 ccaagaacaa gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct
     8821 atccattgag ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag
     8881 ccctgcctct acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt
     8941 ggcttatcag cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt
     9001 taaagaaaat aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca
     9061 tgatttatct gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa
     9121 tttagctgaa cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg
     9181 aatcgtaatt gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc
     9241 agaaatcact ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat
     9301 cgaaaagacg aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt
     9361 gacttcgtac ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta
     9421 agtacacagg cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc
     9481 ggaagcgaaa aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat
     9541 ttgatgattg ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact
     9601 tatacgatta tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg
     9661 acttctgtcg aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt
     9721 catttgaatg cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg
     9781 aaagtactta ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc
     9841 ggttttggca tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt
     9901 gatatcagcg gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag
     9961 cgggaactga gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca
    10021 aatgaagcag acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac
    10081 atatttaaag ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg
    10141 catctgatga atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag
    10201 cttcgtctgc tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca
    10261 ctgatctttt ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag
    10321 tatggaagac gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct
    10381 ctaaacacgt ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac
    10441 aaaaatttga aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg
    10501 tcccagcctc ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa
    10561 gccgctgatt tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt
    10621 ccgatatagg caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat
    10681 gcgctagaag ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct
    10741 gctggcgtac gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt
    10801 tcgcgtaatg gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg
    10861 atattgtatt gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt
    10921 gccgcagcat aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg
    10981 atgattttac cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat
    11041 ggggcattta aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc
    11101 ggtgtccgtt tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct
    11161 ttgatctgtt gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct
    11221 tttgcatcga tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc
    11281 aagttattaa atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca
    11341 ataccataga cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata
    11401 ctaaattcca tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc
    11461 catgtctgta taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt
    11521 acctacacct gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac
    11581 taaatgtttt gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc
    11641 ataatcaaaa tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt
    11701 ccaagcactt cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa
    11761 gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga
    11821 aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca
    11881 acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac
    11941 aaa
//
LOCUS       DAHEYX010000075        11829 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHEYX010000075 DAHEYX010000000
VERSION     DAHEYX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.076"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            189..1004
                     /gene="thiD"
                     /locus_tag="MB219_001410"
     CDS             189..1004
                     /gene="thiD"
                     /locus_tag="MB219_001410"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCC8427133.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1333..2784)
                     /gene="cls"
                     /locus_tag="MB219_001411"
     CDS             complement(1333..2784)
                     /gene="cls"
                     /locus_tag="MB219_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCC8427134.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3125..5791
                     /locus_tag="MB219_001412"
     CDS             3125..5791
                     /locus_tag="MB219_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCC8427135.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6155..6907
                     /locus_tag="MB219_001413"
     CDS             6155..6907
                     /locus_tag="MB219_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCC8427136.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6929..8083)
                     /locus_tag="MB219_001414"
     CDS             complement(6929..8083)
                     /locus_tag="MB219_001414"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCC8427137.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8268..10709
                     /locus_tag="MB219_001415"
     CDS             8268..10709
                     /locus_tag="MB219_001415"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCC8427138.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11039..11743)
                     /locus_tag="MB219_001416"
     CDS             complement(11039..11743)
                     /locus_tag="MB219_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427139.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3814 a         2104 c         2501 g         3410 t
ORIGIN      
        1 attattagaa tagcgaatac aaataaacga ccactatttt ctgatttatt gcaaatgttt
       61 tttcaaataa atttgattac caagaaaagg tagtggtata tttactgagg gatactttga
      121 aaaacatatg atagaaacag taacagtatt atgaaaaatc tttttataaa aatggaggaa
      181 tcgatttgat ggaagatcag tatgtacaag caatgacgat cgcaggatca gatagcgatg
      241 gaagcgcagg gatgcaagca gatatgcata cattctttac aagaaatgtt tacggtgttt
      301 ctgtcatgac cgcatgtgtt gcaggtaatt cttacggaat cggagcaagt gtgacattgc
      361 caacagattt tatcgacaaa gaatttgaac tgattgcgaa ggactttcaa atacgtgcag
      421 ctaaaacagg tatgctagca gattcaaagt tgattgagac agttgtaaaa aattaccaaa
      481 aatatgattt tggaccatta gtcgtggatc ccgtaatcgt aacgaaacat ggaaatcttt
      541 tactagaaga atctgctttg caaagcttga aagaaaagct tgtccctttg gccgaagtac
      601 tgacacctaa tttttatgaa gcagaaaagc tgactggacg gacattagca gctgaagaag
      661 attttgtgca agcagcaaaa gaactgcaag atatgggtgc aaaaaacgtt atgatcaaag
      721 gacatcatgc taaaaatgcg cagcaagaaa cagtcaatga ctacgtattg ttcgaatcag
      781 gaaaatcatt ttggttaaat gcaccatttt atcctaccga ccggatcaac ggaactggcg
      841 attcgctatc tgcatgtatt gcagctgagt tagcgaaagg aaaaacagtt gaacaagcga
      901 tccgattggc aaaagactat gtaaaccgtg caattcgtga agaaataaat gtagggcaca
      961 aatatggccc catcaatcat tgggcagctg gtgaattaga gtaaaagaaa gaagttgtga
     1021 aaaagttgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     1081 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtc
     1141 gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag cttctcatgt
     1201 ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tgttcggaag
     1261 aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt tttctctact
     1321 tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct tgaataatag
     1381 ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg tctcatcttc
     1441 tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag cacttgtttc
     1501 aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc agatttcatc
     1561 atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga tttgtacatt
     1621 ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag gatgatccgg
     1681 tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt ttaaagcagc
     1741 aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt ttttagcaga
     1801 agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg gtccgcttga
     1861 aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt ttttaaagaa
     1921 ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc agtctgttaa
     1981 gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg tatcgcgcca
     2041 atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac caccggtata
     2101 actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca atcggaaacg
     2161 aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt tgattagacg
     2221 attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata ttcgaacttc
     2281 cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga tattatctgt
     2341 cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat cttgaagcag
     2401 tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc ttgtcagcgg
     2461 catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat cgcccatgtc
     2521 aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta attctttcaa
     2581 gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga aaaataaata
     2641 taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc aaatggctgc
     2701 tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta atagataaag
     2761 ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata acattagtat
     2821 agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct ttcatgaaaa
     2881 tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag gatattagta
     2941 cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat atcaacagta
     3001 agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac aaatgcgtat
     3061 ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa atgggggatg
     3121 aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga tcgactcgtt
     3181 gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga aggaaaaatt
     3241 agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt ggaaattgtt
     3301 cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac tgattcagat
     3361 ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt tgaattctat
     3421 aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga aaaacctttc
     3481 agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg caaacgaact
     3541 tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg atggacgttt
     3601 actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt cgacttctgc
     3661 agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta caaatatggt
     3721 attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt caactgggaa
     3781 taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc aagtcactcc
     3841 gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt tggcattgtc
     3901 tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta caggagatat
     3961 gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag ctgctattcc
     4021 agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata aaatggcaaa
     4081 acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga caagtatcat
     4141 ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg actacttttt
     4201 aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag ctgacgaacg
     4261 tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag acggtactaa
     4321 attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa accagcctta
     4381 tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact cagatcgaaa
     4441 attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta aaggcggacc
     4501 agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg ttccgatgac
     4561 ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac gcgcattacg
     4621 tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg aagatgaaga
     4681 cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg aagtgactca
     4741 ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg gtgaccacaa
     4801 aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa acctggcttt
     4861 gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt tagaaaaagt
     4921 cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg cttggcaaaa
     4981 taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag cattgaaaca
     5041 agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg cagcagcgat
     5101 ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag gacgtaatgt
     5161 gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag gtgcgatcat
     5221 tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat tacaactgtt
     5281 gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag aaaaagctga
     5341 acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta cggacaaaac
     5401 attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct ttgctcaatg
     5461 gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa cattgatttg
     5521 gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta tccaagcagg
     5581 gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc tttactgctt
     5641 aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg gattgacaga
     5701 agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa tcactaaatt
     5761 gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt gagcctgccg
     5821 tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt gttgaatatt
     5881 gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt tgtaaaatca
     5941 gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg aaagagggac
     6001 tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa agccaatcat
     6061 tttattattc atcaaaagag atagaataga taaaagagga gaatgattta ttccgaaaag
     6121 tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat gggatgaatt
     6181 tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg aagatgtaaa
     6241 aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg ttgctggagg
     6301 atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga tcgatttttc
     6361 ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc caaatgtttc
     6421 ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg aaatggtttt
     6481 tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga tgaaaaagtc
     6541 aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt tttatcctat
     6601 cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg taatggatca
     6661 atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt ttacttattt
     6721 tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt acgcaaaaga
     6781 tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa tccttagtac
     6841 gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa cctttcacga
     6901 ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata tcattttcga
     6961 tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg tgttcaggaa
     7021 gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga gaatctagca
     7081 tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga taacctgtaa
     7141 aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct cctggctgta
     7201 gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca gtgtaaagat
     7261 taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga cgttcctgat
     7321 tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc ttagtatacg
     7381 gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat tctttatgtc
     7441 gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga ccatcaacaa
     7501 caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc agaaatacgc
     7561 tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca acagcaccga
     7621 caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca cttgctatga
     7681 ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc aaagctgcgg
     7741 tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg attttcagta
     7801 aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct gttccgacga
     7861 tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca gcatttgctc
     7921 ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga ccaatcccta
     7981 tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata tctaaatata
     8041 aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca cctctttact
     8101 tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa tataaaaaag
     8161 agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa caagagctat
     8221 ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg
     8281 ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta agtccattta
     8341 ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca aaactgaaaa
     8401 aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat ggtcaaaatg
     8461 ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca atgacgatca
     8521 atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg atccaattag
     8581 gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca cctatgctga
     8641 aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg
     8701 gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat gttgcaacac
     8761 aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag
     8821 cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag atttctgatg
     8881 aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa aaactgggca
     8941 cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct tccccgttga
     9001 atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat aataatctgc
     9061 cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt atcatgggaa
     9121 tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat ttatcaacaa
     9181 tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt agccaagaac
     9241 tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta ggccatccaa
     9301 ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt cgtgaacaga
     9361 agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca gtcttgttag
     9421 aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat cagcttacgc
     9481 catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa actgcgttga
     9541 tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa aaccaagtga
     9601 gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc aagaaaaaat
     9661 ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa
     9721 tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa atcgttctat
     9781 caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa gaagagctga
     9841 ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt gttcaagata
     9901 tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat tttttagtgg
     9961 atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt gtggcaaaca
    10021 agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt aattttgcta
    10081 cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg agaaacaaag
    10141 aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc
    10201 cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct gtagtggcag
    10261 ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg
    10321 gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt ggaaaactaa
    10381 cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca aaagcaaaag
    10441 catctttagc tatgttagat attgattcag ccaaggaact agcacaagta attgctgcag
    10501 tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga atccaaaaag
    10561 gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt ggtgaggaaa
    10621 tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag acagctatac
    10681 aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga ataaagcaag
    10741 atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa aatatgttta
    10801 ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa ctccaagata
    10861 tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt gttggacagg
    10921 tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct gtagaaatta
    10981 ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa tagaattttt
    11041 atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa gtcaatttga
    11101 ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta gtcatagaaa
    11161 tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact tcatgttcat
    11221 tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca gcaaaactta
    11281 aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt ggtttttccg
    11341 ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca gcttgcgcag
    11401 ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca ataagaagat
    11461 tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt aatacagtat
    11521 tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc ccagctaatc
    11581 cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc aaaccataac
    11641 gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca tccaatgaag
    11701 gacggaattg atttaataat ttttgtttct gttttttctt catatgttct ctccttcgct
    11761 catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg caaaaaaact
    11821 tgcgtatac
//
LOCUS       DAHEYX010000076        11765 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHEYX010000076 DAHEYX010000000
VERSION     DAHEYX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.077"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..181)
                     /locus_tag="MB219_001417"
     CDS             complement(<1..181)
                     /locus_tag="MB219_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427140.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPY"
     gene            319..1833
                     /locus_tag="MB219_001418"
     CDS             319..1833
                     /locus_tag="MB219_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCC8427141.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2236..3219)
                     /locus_tag="MB219_001419"
     CDS             complement(2236..3219)
                     /locus_tag="MB219_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCC8427142.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
     gene            complement(3224..3871)
                     /locus_tag="MB219_001420"
     CDS             complement(3224..3871)
                     /locus_tag="MB219_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8427143.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(3956..4588)
                     /locus_tag="MB219_001421"
     CDS             complement(3956..4588)
                     /locus_tag="MB219_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCC8427144.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(4604..5155)
                     /gene="coaC"
                     /locus_tag="MB219_001422"
     CDS             complement(4604..5155)
                     /gene="coaC"
                     /locus_tag="MB219_001422"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCC8427145.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5152..5889)
                     /locus_tag="MB219_001423"
     CDS             complement(5152..5889)
                     /locus_tag="MB219_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCC8427146.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            complement(5886..6875)
                     /locus_tag="MB219_001424"
     CDS             complement(5886..6875)
                     /locus_tag="MB219_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCC8427147.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7133..7675
                     /locus_tag="MB219_001425"
     CDS             7133..7675
                     /locus_tag="MB219_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8427148.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            7692..8777
                     /locus_tag="MB219_001426"
     CDS             7692..8777
                     /locus_tag="MB219_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427149.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            8789..9595
                     /locus_tag="MB219_001427"
     CDS             8789..9595
                     /locus_tag="MB219_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427150.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            9596..10423
                     /locus_tag="MB219_001428"
     CDS             9596..10423
                     /locus_tag="MB219_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427151.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            10420..11493
                     /locus_tag="MB219_001429"
     CDS             10420..11493
                     /locus_tag="MB219_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8427152.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3651 a         2125 c         2472 g         3517 t
ORIGIN      
        1 aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt
       61 ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc
      121 cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca
      181 taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct
      241 atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag
      301 gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt
      361 cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg
      421 aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa
      481 atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct
      541 atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga
      601 ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa
      661 gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga
      721 acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg
      781 ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag
      841 aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg
      901 aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag
      961 aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag
     1021 gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt
     1081 gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga
     1141 gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa
     1201 attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa
     1261 ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat
     1321 aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa
     1381 ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact
     1441 ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa
     1501 acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc
     1561 acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga
     1621 atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa
     1681 aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac
     1741 aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata
     1801 caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt
     1861 gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag
     1921 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta
     1981 tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     2041 gaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     2101 acggcggaag cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     2161 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     2221 ttgatcagca tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc
     2281 tttcatatat tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg
     2341 ccgaatcttg ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc
     2401 tgctttatac tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc
     2461 aatcaataca ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac
     2521 ccaacgtcca tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa
     2581 atccaaaata acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc
     2641 ttgtttttta tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc
     2701 ttctgatcct gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc
     2761 tgctgttccc acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt
     2821 tccaagccag taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga
     2881 aacatagtct agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc
     2941 ggcataacca cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat
     3001 atcactttta ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg
     3061 tttcaaagag actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac
     3121 tttgatcagt aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac
     3181 atctacagaa ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc
     3241 attcttggaa tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag
     3301 aaatatccgt ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg
     3361 tttttgatgc cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag
     3421 tcaatgtgac tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg
     3481 cataatgttt agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg
     3541 cataaacagc actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg
     3601 ttttgattgc gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa
     3661 aaatcaatac atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat
     3721 caaaagagac atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca
     3781 cccctgcaaa aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt
     3841 gtccgtttgc acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt
     3901 ccattcaata gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt
     3961 tagtaagaga agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa
     4021 gacctgcgac gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat
     4081 aaacagcatt taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga
     4141 tcatattcat cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag
     4201 aaacggaatc tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa
     4261 ccaccccaat caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt
     4321 ctgttccgat gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg
     4381 tgctacggat catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc
     4441 ctaaaacgat cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga
     4501 tagggataaa acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag
     4561 ttaatgtaaa gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat
     4621 taagagtcag catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa
     4681 aatctccaca agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca
     4741 atgtttccag attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg
     4801 caatcaattt tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg
     4861 caatgccgtt tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt
     4921 cagcttgttt tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt
     4981 ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc
     5041 tgcttttaga cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat
     5101 cagcgctttt gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga
     5161 ttcctccagt ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagtttc
     5221 ttccactgtt ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt
     5281 caaatcattt gcgaaaataa aatcagtttt gttatttatc agacaatttt ttgcgactgc
     5341 cagaagagat tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg
     5401 ctgccattca cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg
     5461 ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca
     5521 attgtccagt tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga
     5581 aaaactaaaa gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg
     5641 ttcttttttc gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata
     5701 aatcgttata ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat
     5761 tgtggtatcc ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga
     5821 atgatttgta atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag
     5881 aatcttcatt tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt
     5941 agtttagatg ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct
     6001 gcaatctgca aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg
     6061 acagaatcaa ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt
     6121 agtttgattc ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga
     6181 agaagactgc ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt
     6241 cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct
     6301 agacttttta gccatgtgac agactcttct tttctggaaa aggcaatgat tgactggaat
     6361 cctagtttat gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg
     6421 acaacttttt tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca
     6481 gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga
     6541 actaaccagt ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca
     6601 agcccaaaac cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg
     6661 acgacagttc cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca
     6721 gactcgctca ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag
     6781 acttcatttt cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt
     6841 tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt
     6901 tcttttatca taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa
     6961 aattaggtga aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat
     7021 tcagcaaaaa aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga
     7081 gtggtagtaa acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat
     7141 tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga
     7201 acgcacagat ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc
     7261 aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca
     7321 tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga
     7381 tgaagaaaac gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga
     7441 gcctgtcttg ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc
     7501 tgaaacgttt atttttgtat tagaagggga attagagtta agcttaggag agacggtcta
     7561 tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa
     7621 tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag
     7681 ggggtaaaaa cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg
     7741 atgaaacagt attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc
     7801 ttggtccttc cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag
     7861 ctacagaagg ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc
     7921 gacaagtaaa tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata
     7981 acgttgcttt tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa
     8041 aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt
     8101 ctggtggtca aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat
     8161 tgctattaga tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg
     8221 aactgcggga tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag
     8281 aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa
     8341 gtggtacacc cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg
     8401 gggagagcaa tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta
     8461 agcaatttga atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac
     8521 gcccagagga tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata
     8581 ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat
     8641 ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc
     8701 cagaagatat ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg
     8761 acagctacga agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc
     8821 ttatattttg tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc
     8881 tttttttgat atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg
     8941 gacatcgctt tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac
     9001 gctgatcatc agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg
     9061 gctgatgctg attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat
     9121 tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc
     9181 gaaacagata ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc
     9241 atttatgatt atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc
     9301 tagtagagat ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc
     9361 attaaatggt gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat
     9421 gctgactcgt ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca
     9481 tttcttggtt acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc
     9541 tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa
     9601 aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat
     9661 cttctatctg attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt
     9721 tacttgggaa aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa
     9781 tacgttcatg ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc
     9841 tatgggaatc tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa
     9901 catcttatta gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact
     9961 actaggattc ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc
    10021 aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc
    10081 ggctagagac cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct
    10141 ttctccagga atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc
    10201 ggttacattt ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg
    10261 tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc
    10321 catgatattg gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa
    10381 gaaaaacaga agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg
    10441 tttttaggta ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa
    10501 acaagtggca tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat
    10561 cctagtttga tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt
    10621 gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct
    10681 ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat
    10741 cattcgaaga tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat
    10801 ccagataatc agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat
    10861 aaattctttg atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa
    10921 gacagtttga tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta
    10981 ggctattctt tgaatagtaa gaatatggca cagctgacag aagcagcaaa taaattgaat
    11041 catttaacac caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat
    11101 gaagaagcct ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat
    11161 gaacatttac attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt
    11221 atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa
    11281 ccggaaaatg cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca
    11341 aaagcgttat tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg
    11401 atctcccatc ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac
    11461 ctattcttag aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact
    11521 tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta
    11581 ccaaaagcgg atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc
    11641 taccagagaa aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct
    11701 aagattgaaa aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc
    11761 ttctg
//
LOCUS       DAHEYX010000077        11623 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHEYX010000077 DAHEYX010000000
VERSION     DAHEYX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.078"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(379..2388)
                     /gene="metG"
                     /locus_tag="MB219_001430"
     CDS             complement(379..2388)
                     /gene="metG"
                     /locus_tag="MB219_001430"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCC8427153.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2656..2934
                     /locus_tag="MB219_001431"
     CDS             2656..2934
                     /locus_tag="MB219_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427154.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3031..3852
                     /locus_tag="MB219_001432"
     CDS             3031..3852
                     /locus_tag="MB219_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCC8427155.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4137..5147)
                     /locus_tag="MB219_001433"
     CDS             complement(4137..5147)
                     /locus_tag="MB219_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8427156.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5159..6112)
                     /locus_tag="MB219_001434"
     CDS             complement(5159..6112)
                     /locus_tag="MB219_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8427157.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6102..7172)
                     /locus_tag="MB219_001435"
     CDS             complement(6102..7172)
                     /locus_tag="MB219_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427158.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7178..8221)
                     /locus_tag="MB219_001436"
     CDS             complement(7178..8221)
                     /locus_tag="MB219_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427159.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8222..9163)
                     /locus_tag="MB219_001437"
     CDS             complement(8222..9163)
                     /locus_tag="MB219_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427160.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9247..9594
                     /locus_tag="MB219_001438"
     CDS             9247..9594
                     /locus_tag="MB219_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCC8427161.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9664..11325)
                     /locus_tag="MB219_001439"
     CDS             complement(9664..11325)
                     /locus_tag="MB219_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8427162.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3304 a         2344 c         2102 g         3873 t
ORIGIN      
        1 tgaacataaa gtgatagaac ttataattaa cacatcaacg ctactgttag atataaatat
       61 agatgtaata aagtcattgc attcaaactt tatgaatgta aggacaataa aaaagagtac
      121 cagatgaatc aattaaaatc tgcccgaccc tttatcatta tgcttttttt ctgtttttac
      181 gattcctatt agtattattc taaattaaca aaaatcagcg aagctcgtac ttctttttct
      241 aggatttatt ttggatagtt ctctgtttct gtattatatg attggcttca agtaataaga
      301 aggaatttac gaaaattgct tctcaaattt cggaaaaagg gggtatgaca caactagtgt
      361 cataccccct ttgaatttct atgcgatttc tgagccattt ggcatggatt ttggtgcttc
      421 gattacttgt aaagagccat ctggtgcttc tgcagaaagg atcattcctt gactgatttg
      481 tccacgcatc tttcgtggtt tcagatttgc tacaatgacg acttttttcc cgatcaattc
      541 gcttggatca ggataaaatt cagcgattcc tgacaagatt tgacggtctt ggctgtctcc
      601 agcatctagt cggaattgca gcaacttatc tgccccttct actttttgac agttgatgac
      661 ttctgctact tttagttcta ctttttcaaa tacatcaaat ttgatttgtt tttctttcgt
      721 tgaaacaagt tctgtttctt ctggatccca tttgactgta tcttcgtttg tttgagtacc
      781 ttcagacatt ttttcttgaa taaaagcaac ttcttcttcc atatctaaac gtgggaagat
      841 tggtgtacct tttgcaacta cttttgttcc tgaaggaaat tcgccaaagt gtaatccttc
      901 aagattcatt gtttcagagt ccaatcctag ttgattgaag atcttctctg gcgtttctgt
      961 catgattggc tgaagtaata tcgctgcgat acgcaaagat tcagctaaat ggatcatgac
     1021 actattcaat tcagctttct tttcttcgtc ttttgctaat acccatggtt ctgtttcatc
     1081 aatgtatttg tttgcacgag aaatcagcac ccagatttca gccaatgctg tattgaattc
     1141 cattttttcc attgcatcat ggtaacgtcc gacaacgtta gcagccgtcg tagacagttc
     1201 actatcaaat ggagtgacaa gtgaggcata gtcaggaacg agtccatcac aatatttgtt
     1261 gatcatagca atcgttcgat tcaataagtt ccctagatca tttgccagat cataattgac
     1321 acgggaaaca aaatcttctg gcgtaaagac accatctgac ccaaacggaa cagcacgaag
     1381 caaataataa cggagtgcat ctaaaccata acgttccaca agcatttcag gataaacgac
     1441 atttcctttt gatttagaca tttttccgtc tttcatcaat agccagccat gcccaaatac
     1501 ttttttaggt agaggcaaat caagggccat cagcatgatt ggccaataga tcgtatgaaa
     1561 gcgaacgatt tctttaccga ccatctgaac atctgcaggc cagtattttt cgaatagact
     1621 ttcatcttcg gagccatatc ctagtgcagt aatataattg gataatgcat cgatccatac
     1681 atacaccaca tgtttcggat cactcttgac tggtactccc caagtaaaag ttgttcgtga
     1741 aactgcaaga tcctctaatc ctggtttgat aaagttgttg atcatttcgt ttttacgaga
     1801 ttctggctgg atgaattcgg gatgttcttc atagtattcc aatagacggt ctgcgtattt
     1861 gctcatgcgg aagaagtatg attcttcttt taccaattct acttcgtgtc cacttggcgc
     1921 tttaccaccg atcacgttac cttcgtcatc tttgtacact tctgcaagtt gtgtttctgt
     1981 aaagaattct tcgtcagaaa cagagtacca gccttcatat tcacctaagt aaatatctcc
     2041 ctgttctaat aaacgatcaa atattttttg aacagctgct ttatggtaat catctgtcgt
     2101 gcgaatgaat ttatcataac tgatatcaag cgttttccat aatttcttga catcagccgc
     2161 cattttatct acatattctt gcggttttac acctagttct tctgcttttg tttcgatttt
     2221 ttgaccatgt tcatccactc cagtgagata aaacacatca aagcccatca accgtttata
     2281 acgagcaatt gcgtcacatg caatcgtagt atacgagttg ccgatatgaa gtttgccgct
     2341 tggataatag attggcgtag tgatataaaa agtttccttt tctgccacgg ggctcttcct
     2401 cctttatcgc atgatgcgaa ttcgtttcct tgctagtata ccataaaaaa agcatgctgt
     2461 tagctttttt tcaaaatgaa aaagactgga taaacgactt ctatctacgt tcaatctatc
     2521 ctttaaaagg atgaataggt tgaataaatg cgggtttttg ttttgtttca tatctactaa
     2581 aaaacggctt tttatttctc ccctcacacg tggtataata aagactatct atcttagtaa
     2641 gggactggaa caatcatgca gaagaaacat ctttttttta cacttagtat tgcttttctt
     2701 tcattagcac atttaatatt cagctatttt tatattcgta tgtatggtta ttttaacctc
     2761 catgggcatt tgaacagttt tatgacagca gcttggattt tgcgttttat cattgacgta
     2821 tatatcgtta tttgtggctt ctttgccatt cgtgaagagc ggtataaagt attgccgttt
     2881 tatctgcttt tctttttatt caatctcata cttccattta tttttcatat ttaaatgata
     2941 acctagattt ttcaagtaaa tccttggttt tttttcagag tctgctacaa taaggtaaaa
     3001 agtactttta ctgtcaatgg aggtggtcag atgaaccgtt ttgcttctaa agaagaagaa
     3061 aaattatatt atgaacataa agcaagtgaa gaagaatttc ttaactggta tcaaaaacag
     3121 gaacgtccag aatatgacaa gccctctttg actgtcgata tcgtactgat gtgctataac
     3181 aaagaagaag atcagctgaa agttttgttg atccaaagaa aagggcatcc ttatcgaaat
     3241 tcatgggcat tgcctggtgg ctttgtccaa aaggatgagt caactggaga aagcgtctta
     3301 agagaaacaa aggaagaaac tggtgtggtt atttcaaaag aaaatattga gcaactgcat
     3361 acattcagta cgcctaatcg agatccgcga ggctgggtcg tcacagtcag ctacttagcc
     3421 tttattggag aagaaccgct aatcgccgga gatgatgcca aggaagtacg ctggttcaca
     3481 ctagaacgtc atagtaacaa gatccattta tctagcggag aagtggctat ctcgcttgat
     3541 ttagtcactg gagagtcttc tggcaaagat actttggctt ttgaccatag tcaaatcatc
     3601 ttgaaagcat tcaaccgtgt agtcaataaa atggaacacg agccgcaagt tctacaagtt
     3661 ttaggtaaag atttcacgat tacggaagct cgaaaagtat ttgctaaatt cttagggatc
     3721 gattataaaa cgatcgatca ttccaatttt aagaaagcta tgctccaata tttcgatgag
     3781 atcggtgaac gtcctgtagg aatcggccgt ccttcaaaaa tctatcagct caagccgggg
     3841 caccatgaat aaggttgtga gcaaaagatt cagacctaat cattcaagaa aaaacgcaaa
     3901 aacaactctt tgtgagttac gttttttgtt tcagaatttt gatgtattcg taaactttct
     3961 gaataaaaaa ttaacaatag cagaaaaagc tcgtacgaac tttttctgct attgttttat
     4021 tggtttgtca taattagtga gcttttcttc aaaagaaaaa ttcctagaaa caaaagtcat
     4081 taagactttt tattaacaag gctaacccta acgctcctat cagtttcatt cattttttat
     4141 cccttgataa ccgaatgtct gcccattttt tctactactt ggctctttct tacggaattg
     4201 ctttcttctg ctccgtcggt attgatcaaa agaaattttt tattatacca ttgggactta
     4261 taacgcaatt ttgttattaa tttatgccag tcgtgaatag aaaaatcagc atgtgcctct
     4321 tcttttggaa aagattctac aaataagtag tcatattgtt tgaccaattt cccagataat
     4381 tgttcaagat aatcttctcg ctggttcttt tgtttcagat aaagatccat tacttttctc
     4441 ttttgtttct gataattgct tgcctgatct aacctgatct ttctatggtg agcggagcgg
     4501 actttcacat tcaatttccg ctgtgcatgt tgcagctttt cttctgtctg gtgaaatttt
     4561 ggtaaagtta cttctagagg tcgagatgtt tggatcaatg tcttcggatg ataggcgatc
     4621 ccgatccatt tagctaaaga ttcttttttt actggcgaaa catcacaaag tagtgcgaca
     4681 taaaattctt cattatatcg agcagaaatc gtagcggagc gaatcgatcc ttcaactggc
     4741 cgatgctgat ttatcttgat tagttctttt tgttttggta actttaagta gccattttcc
     4801 agatagatcg tacgggtttg gttgttggtc gtataagatt gccacatact ttttttcgtt
     4861 ttaagctttg ggaagctggc acgtcccttg aaataattgc gaaaagcccg atccaaatta
     4921 agctgtgcat ttgctaaagc aagactgtct acttctttta agaatgggta ttcttttttt
     4981 aatgtagcgg gtgtgagcga aaaattggct actcctgttt gttgccaact tttctgccgc
     5041 tttttcagca aatggttata ggtaaatcga gcacatccaa aagtacgaat caattgctgt
     5101 ttttgttctt ctgtcggata catacggaat ttaaaagctt ttagcatcta tgtccctcct
     5161 agttagcaac cagaagagat tccttttgtc gttttagttt ttttgcgtaa tatgggactt
     5221 cctgctcagt tgcatagaca aaatgttctg gtgcgatttc acgaagttga cgtacttgtc
     5281 cgtcatctgg ttcaggttga tatttgattc ttctgcgttt tctctcatgg tctggatctg
     5341 gtagaggaat agcagataac aaactttctg tataaggatg aacaccgtga ttatagattt
     5401 catcacttgt tcccatctct aataacaagc cgttatgcat tacaccgatt cggtcactaa
     5461 tatgtttaac catcgacaaa tcatgtgcaa taaatagata ggtcaattga tgttcttttt
     5521 ggagatcttg caacaaattg accacttggg cttggatcga cacatctagt gcagaaattg
     5581 gttcatcaca aatgataaaa cgcggtttta ctgccaatgc tcgtgcaata ccaatacgct
     5641 ggcgttgccc acctgaaaat tcatgaggat aacgagtacc gtggcttgga ttcaatccaa
     5701 cagttctcaa tagatcatcg actttttttg cacgctcttc agatgattta gctagtccat
     5761 taacatcaat tccttcggca atgatatctc tgactttcat acgaggattc aacgaagcgt
     5821 atggatcttg aaagatcatc tgaacatcat gccggaattt cgtcaaacct tttttatctt
     5881 tgatcttcgt tatatcttga ccgtcgaata atatctcacc atcagtaggt tcattcaagc
     5941 gaatgatcgt acggccagta gtggattttc cgcttccaga ttctccaact aaaccgaatg
     6001 tttctccttc ataaatgtga aagctgatgt catttacagc atgtacttca ttcttttttc
     6061 ctacattgaa atactgtttc aatcctttaa cttcaagtag tttatttggc atcagcaatt
     6121 cctccttgac cgtaagtatg tttttgtttc tctttaaaga tagcccagcg acgttggatt
     6181 tcttctggcg gtgtcacttt gggtgcctgc ggagcaagta gccaagttgc tgccttgtgt
     6241 gtttttgata gttcaaaaaa tggtggttct tgttctgcat cgattttcat cgcgaattca
     6301 tttcttggat agaatgcgtc tccttttggc gggttcaata aatccggtgg agaaccagga
     6361 atagcataca atcgatcgtt cgcactatct aatgtcggca ttgaaccaag cagtccccaa
     6421 gtatagggat gttgcgggtt gtagaaaatt tcttccgatg ttccaacttc aacgattctt
     6481 cctccataca tgacagcgac acgatcagcg acattcgcta ctacaccaag gtcatgagtg
     6541 ataaagataa ttgatgtatc gatttttgtc tgaatttctt tcagcagctc caaaatctgt
     6601 gcttgaatag tcacatcaag cgctgttgta ggttcatcag caatcaggat ttccggataa
     6661 cataccagtg caatcgcgat cacgatccgt tgacgctgac ctcctgagaa ctgatgcgga
     6721 taactgttga tacgtttctc agcttcgggt attccaacta atttaagcaa ttctaatgca
     6781 gctttttgtc cctctttttt tgaaacttta ttatgctttc taagggactc agcgacttgc
     6841 ttcccaatgg gcatcgtcgg atctaaagag gtcattggat cttggaaaat catcgctatt
     6901 tctttaccac ggattttttg catctctctt tctgattttc caacgatatc gttaccttta
     6961 aagaggattt gaccagcatc tatattggcg ttgcttgaca gcaagcgcat gatgcttctg
     7021 gtcgtcactg attttccact tccagattcc ccaacgatcg ccaatgtttc tcctttattc
     7081 aaatgaaaat tgacaccgcg aatcgctcgt acctttccag catacgtatc aaaagagatt
     7141 tctaagtctt tcacttctaa aattttcgtc atcgtcttca ctctttcatt ttaggatcaa
     7201 aggcatcacg caatccatca gcaagtaagt taaagccaat catgataacc gataaagtta
     7261 ctgccggaat ccataataga taaggcagat acaggaatgt cttgtaccct tcgctcaaca
     7321 tcgttcctaa tgaagctgac ggaggcgtaa gacctaaacc gataaagctt aaaaaggctt
     7381 cgaagaaaat agcagatgga atactaaaca tcatttgaat aataatgaca ctagagatat
     7441 tcggcaaaat atgtttgaat gcaattttca ttcttgattc acctaatgtc aatccagcta
     7501 aaacatattc ttgatctttt aatttcattg tttgtgcacg aacgatacgt gccattggta
     7561 tccagtttgt gatggccata gcagccacga ttgaaaaaat tcctggttca aaaacgacca
     7621 acatcaaaat catgacaacg aggtttggaa ttcctgataa gatttccaaa aaacgttgca
     7681 tcacgttatc gacacgtccg cctaacaaac cagaaaccaa cccgtaagct acaccaatcg
     7741 ttacatctag taatgcagca ataaatgcga tcagcaacga gattctcatt cccataaata
     7801 agcggcttaa tacatcacgt cccaaaccgt ctgttcctaa aaagaaattt acgtttgaag
     7861 ggacgtttgc ttgagcatat ttatcaacaa gttcaccacc aaccattgct tttccattca
     7921 atccatcgat attgattccc ggaatacgag gaggcaaatt gatataagcc acgttttggg
     7981 cagttggatc atgaggagaa acaaagattg tgatcacaga gatcaaaatg atgaccaata
     8041 gtaaaaccat tgaaataacg gcagctttgt ttttctttaa acggcgccat gaatcttgga
     8101 taaaattcaa agaaggtgca gtaatctgtt cacgttcttc ttcggtattt ttttgtaatg
     8161 gaagaaattc atccgcaggg atatctgcaa ctgtttcata acgctttggt atcatttcca
     8221 tttatcctcg gctcccttct gaaacacgaa ttcttggatc aactagaccg tacaatagat
     8281 ccacgatcaa aataacaaaa atcagcataa atgagtaaag gatcgttaca gccatgatcg
     8341 taggataatc atttgttgtg attgacttaa cgaattgttc cccgatacca ggaatagcaa
     8401 agatattttc taccaccagt gatccggtca tcaatgctac agctaacggt cctaataaag
     8461 tcatcaatgg aatcaaacta tttcgaagac catgtttaaa ggcgatttcc caacggctca
     8521 atccttttgc ttttgctaat tccacataat cactatgcag tacttccacc atttccgtac
     8581 ggataaaccg agcagagtca gcaagcggcg acatggccaa cgcaattgtt ggtaagattg
     8641 tgtacacaaa gccatcccat tttgcgattg gcaaaacttg taatttgatt gcaaagatat
     8701 attgtaataa aacagcaaat acaaagtttg gaatggaacg acctaatatc gctacgagtg
     8761 tagaagatgt atctgcccat gtattttgct tcatggcaga gatcgttcct aaaatcgtcc
     8821 ctaggactgt tccaaagata attgcctgta accctaactg caaggatggt ccgattcttc
     8881 cagctaacaa atgtgcgact ggctggtttt taaattggaa ggaaattcca aaatctcctt
     8941 gaagcaaatt agaaagataa atcccatatt gtacgataac tggtttatcc aaaccgactt
     9001 gtttatttag catctcgatt gtttcaggac tcaaacgttc ctgattcgta tatggcgtgc
     9061 ccggcagcaa ttgcatcaag aaaaaggtga ttgttgcaat taaccaaagc gtgatgatca
     9121 tgaaaaagac acgttttaat acatatttga tataactatt cactaatgtt tccccctagc
     9181 ttttgacttc tgacagatgt ttcgctacat tattagataa tgaaccaaaa ataaaggagc
     9241 aatcaaatga aaaaaaaatc tatcccctac gcagtagctt ttctactgat tctggttatt
     9301 ttaataaaaa atctcatcaa ccattccttt acactcattc aattatctaa tgatctattc
     9361 ttatggtcat tgccgtttct gattatcggc ggttttttat gggtcttttc ttcgggtttc
     9421 ttcgatcatt ttcagcgatc ggtccatctt gcacgaacaa ggaaccgaaa gaaaaaacca
     9481 gaattttcct cgctctcgtc tgccagctac ggtatgtaca gcttttggct gatcattgcc
     9541 ggaatactaa ttgctctttc tgctattttc atgctttttt ctttgttagg ataagtgaat
     9601 atgagaacga ggttgcgaca tttgccgcag cctcgttttt tctcatgctc acggtattaa
     9661 cgattattct tcaacgtatg tccatttgta atcccatgaa gcgcctgccg catggtgaac
     9721 gatacctttg attttttcat tcactaggtg accttctgct ttttggtaca ccggaacgac
     9781 acccatatca tcagagatga tcttgattgc tttttggaaa tcatcccaac gggcttgttc
     9841 atcgccagcg tctgttgatg aagctgcttt aactgcttcg tcatattctg cattgctcca
     9901 gcgaccacgg ttgtatgaat tacctgtgac aaacaaatca gtaaagctgc ttgggtctgc
     9961 atagtcagca gcccatccgc ctagtactgc gtcaaaatca cccgaagttg agcggtcaag
    10021 acgtacagag aatggaacag gtgttggttt gactgttacg ccatccaagt tttcttggat
    10081 tgagttttga acatattcga ttactttttt cgatgaatca tcatcagaag ccatcaaatc
    10141 aaattctaaa gaatcaacgc ccaattcttt tttcgctttt tcccaatatt cttttgcttt
    10201 atctttgtca taagaaacta attttccagc ttcttttgca aagtcttcgt tcgttgttgg
    10261 actttttacc atatctgatg ggatcaatcc tgtagagaca actgatccgt cacctagtac
    10321 ttgttttact aaagcttcac gatcgataga ataagaaata gcttttctta ggttcacatt
    10381 gttgaatggt gaattctttt cacgttgatt gaattcgata tagcttgtcc gcgcttcttt
    10441 aacagaagta taagctggat cgtttgcgtt ttgctgtgct aactcaccag acaagatcac
    10501 gtcatctgct tggccatctt tgaataagtt caatgcagta gaagattctt ttactacact
    10561 gactttgatt tcagaaagtt ttactgtatc tttgtcccag taatgatcat ttttcttata
    10621 agtccattct gtatctgttc ctggtccatc aaaatcttct aatgtgaatg gtccattata
    10681 aacagcttta tcacttgcag atgcataggc atccccattt tcttccacta cggattgttt
    10741 ttgtgggaaa aatgatggga aagcaagcag atattggaaa tatggtgttt gtttcgtcaa
    10801 tttgatttct aattcatagt caccaactgc tttgatccct agttctgatt tatctttctt
    10861 tccagctgta atgtcttctg cattttcaac tggcgcatac aggtaagcat attcagaagc
    10921 tgtagctggg tctactgttc gttgccagcc ataaacgtaa tccgctgctg taacaggatc
    10981 accatttgac cattttgcgt cttctcgtaa tttgattttg taagttaatc cgtcttcact
    11041 gacttctgcc aactcgctag ctcctgccgg ctgtggtttg ctgtcttcat caagacggta
    11101 aattccttca taaacgttat tcaatgctgt aaaactgatt gtatctgttg caactgataa
    11161 gtcagctgta ggcatttctt gaggcacgac taagttaaat acgcctcctc catctgctga
    11221 accgcttttt gctgaactac tgcttgtggc tgaactgccg ccataacatc cagcaagtac
    11281 aaggccgcat accgcaacca aaccaaatgt aaatttcttt ttcattttac gtaccccttt
    11341 attggaattt tcagaataat taatattttc tgaagttgtt taagagttta atgaataatt
    11401 attaaaaaat caacactttt ttttgtcttt ttcataaaat aatgattttt tcgtgaggtt
    11461 tcaaggaatt tttcagaaaa aatagctttg tacaaggttt tccatggtct tccaaaagcc
    11521 attaaataat tttcgttata ttgtctatta ttgccataca aagataaaaa agaataaaca
    11581 aaaagaaact gtgacaaaag tcttgtcaca gtcccttatc tga
//
LOCUS       DAHEYX010000078        11606 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHEYX010000078 DAHEYX010000000
VERSION     DAHEYX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.079"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            208..773
                     /locus_tag="MB219_001440"
                     /pseudo
     CDS             208..773
                     /locus_tag="MB219_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1472..1993)
                     /locus_tag="MB219_001441"
     CDS             complement(1472..1993)
                     /locus_tag="MB219_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCC8427163.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            2191..5871
                     /gene="nifJ"
                     /locus_tag="MB219_001442"
     CDS             2191..5871
                     /gene="nifJ"
                     /locus_tag="MB219_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCC8427164.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            6060..6947
                     /locus_tag="MB219_001443"
     CDS             6060..6947
                     /locus_tag="MB219_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCC8427165.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            6944..8113
                     /locus_tag="MB219_001444"
     CDS             6944..8113
                     /locus_tag="MB219_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427166.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            8137..9492
                     /locus_tag="MB219_001445"
     CDS             8137..9492
                     /locus_tag="MB219_001445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCC8427167.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(9564..11369)
                     /gene="glmS"
                     /locus_tag="MB219_001446"
     CDS             complement(9564..11369)
                     /gene="glmS"
                     /locus_tag="MB219_001446"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCC8427168.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         3671 a         2107 c         2554 g         3274 t
ORIGIN      
        1 aaccgattgt taacaatttt ggtgattaga aaacaattac gataaataga aacaagccaa
       61 atcatcagca agtaaaagct gatgatttgg cctttcttat acgagtagta ggaattcata
      121 gcgaatatta ttacgatcag tagatatttt gatattaaag gagtattacg aactagtatg
      181 tgagaatgta gaggtgattt gcgaataatg agaaatttat caaatagaaa caaaatatta
      241 atcattattg ttgttatagc tgtgtttcat ctgggaacga atgcggtttt aagtagaata
      301 atattgggtc ctaagccacc aagacctgcg attacccgag gggagtttga ttttagatta
      361 gaatatgaag tagatgggga aaggattgtg atagaagata caattgttgc attatttgat
      421 ggattttctg cagatgcagg tagcatggca tggtatcgca cgtggcgttt gcatttggcg
      481 agtgacaaga gggcacaaca attattgtta catgagttag atgatggtcg aaaaatatat
      541 tatcattttg gaggagctgg attttatatg gggaagttag tagcagtgta actgtaacaa
      601 ggaaagattt gccaggtctt ggcattcgaa actccatatt ttatatcgag cgtgggagag
      661 atacatctat tgggaacagg aaagggttaa ctcttgaaga gttatataat gactttggaa
      721 tccgtcttgt tagttgggaa cacaatccac caatagaaaa tcgctttgaa taatcgaaat
      781 ctattaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
      841 aaaccattga aatactaggc tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa
      901 ctattaaatg agaggagtta ggagttgtta gctttatacg aatcaaagtg gcaaatacag
      961 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     1021 gaaacaactt gtttaaaata aacgacctag ctcaacaaac gaccatctaa tcaactaaca
     1081 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     1141 ttgggatact agtgtgaatc accgtacata aatattactc gttaagggaa tttgagaaat
     1201 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     1261 tggtggagat aatttgctca gcttgtcata agaaatgaga taatctggca gatgcaagct
     1321 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     1381 aagaaggagt tgcttctgct tccgccgttt ataggtgttt agagcgtgag acaaaagtgt
     1441 tctttacttt tgtcctacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     1501 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtc ggaacaaatc
     1561 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     1621 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     1681 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttga ccatcgctga
     1741 ttgtgagata ccggtgcatc acgtttcctc gctgcaagct ttctttttct ttatcgaaca
     1801 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     1861 cttcaaacca ttgtcggcaa tacggacaag ataggttgat gaattcgacc atttttttag
     1921 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     1981 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     2041 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gacatatgtg
     2101 aaatattttt ataaataaaa gaaaaaatgg aatgttttac gttataatgg aacagataat
     2161 aagagagaat atgaacaagg atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat
     2221 acggcagccg catatatttc ttatgcattt accgagattg ctgcaatata tccaataaca
     2281 cccagttcga ccatggctga attagtagat caatggtcag cagcaggaaa agaaaatgta
     2341 ttcggtcaac cagtaaaagt agtagaaatg caatccgaag caggagcagc tggtgtggtc
     2401 catggatcat tgaaaactgg tgcactgaca actacatata ccgcttctca agggctgctt
     2461 ttgatgatac cgaatatgta caagattgct ggggaactac tgccaagtgt tttccatgtt
     2521 gctagtcggg cagtgacgac aaatgcgttg aatattttcg gagatcacgg agacgtgatg
     2581 gctgcaagac aaacaggatt tgctatgtta gctgaaagca gtgtacaaga agtgatggat
     2641 ttagcacctg ttgcccatct tgcttctatt gaaggagaag tacctttttt gaatttcttt
     2701 gatggattcc gtacgagtca cgagattcaa aaaatcgaag ttttggaata tgaagaatta
     2761 gctcctctga tcaatcagga gaaattagct tcatttcgta aacgagggat gaatcccaat
     2821 catccgactg ttagtggtac gaaccagaat ccagacatcc attttcaaca acgggaaacg
     2881 atcaacccct attatgataa attacctgaa atcgttcaaa aatatatgaa agagatcaac
     2941 cgattacgag gaacttctta tgacttggtg acttattacg gagcagacga tgcggaagaa
     3001 gtcattgttt ctatgggatc agttgcacag acgattgaac aaacgattga ttacttgaat
     3061 gcaaacggac ggaaaacagg ttttttgaat atccatcttt atcgtccttt tccagtggaa
     3121 acattcgtgc aaaagttgcc tcatacagta aaaacaattg ctgttcttga tcgaagtaaa
     3181 gaaccagggg caggtggaga acctctctta ttagacgttc aaagtgcgtt atatgattct
     3241 gaacttcgcc cggctgttat tggtggtcgc tacggattgg ggtctaaaga tgtgactccc
     3301 gaccaaatct cagcagtttt tgatgaattg aaaaaagacc caagcatacg caaaaaacgg
     3361 tttaccatag ggatcgtgga tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg
     3421 gatttgacag aaccgcaaac attccaggcc aagttttggg gatttggatc tgacggaaca
     3481 gttggagcaa ataaatcagc tatcaagatt attggagatc acaccgataa atatgcgcaa
     3541 ggctattttt attatgattc gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt
     3601 ggagatacgc cgatacgttc agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac
     3661 acaccagctt acctgcatag ctatgacttg gtcaaaggat tgaagccagg tggcattttt
     3721 ttattgaata ctctttggag cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa
     3781 cgttacttgg ctgaaaataa cattagattc tatacgatca atgccatgag attggcccaa
     3841 gaagtcggtt tgggacgccg gatcaatacg gcaatggaaa cggctttttt taaattagct
     3901 gacatcattc cttttgatga ggtgcttccg ctgctaaaag aagaagccct gaaaagctat
     3961 ggacataaat caatggctat cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa
     4021 ctgcttcatc aagtcgcgat cccagaccat tggagaacgt tggagatgcc tgaaaaaatc
     4081 aataaaagga acactggtta tatacaagaa atacttgaac cagtgaatgc acaggaagga
     4141 aatcaactct ctgttggtac acttgtagca aatggaatga caactggcga aatgccttta
     4201 ggaacggctt ctatggaaaa gcggggcgtc gctttagaag tacctgagtg gattagtgat
     4261 cgctgtacga tgtgtaatga atgtgccttt gtttgtcctc atgctgccat ccgtccattt
     4321 ttagcagatg aagaggaaat ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga
     4381 ggcgcagacg gcgtaaaata ccgtatccaa gtttcagtgg aagactgtac aggttgtggt
     4441 ctgtgtgtag aggcttgtcc cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa
     4501 cagaaagaac aagctgtgaa ttgggctttt gcaatgacat taagacaaaa agcaaatcca
     4561 gtcaaaggaa agaatactgt cttgtctaca caattcaatc aaccgcttct tgaattctcg
     4621 ggcgcttgct caggctgtgg cgaaacacct tatgtaaaat tattgacaca aatgttcggt
     4681 gatcgaatga tgatcgcgaa tgcgacaggc tgttcttcta tttggggcgg tgcttctcct
     4741 gcttctcctt atacgaccaa tgaatgtggt caagggcctg catggtcaaa ttctttattg
     4801 gaagataatg ctgaatttgg ttatggtatg cttctagcgg cacagactcg tcgcgaggct
     4861 ttggcaagtt caatcaaaga agtattgcca aaagtatcag aagaactacg agaattgatg
     4921 gaagactggt tggtacacat gtttgagagc gaaggaacgc agcaaagagc agcaaaactg
     4981 caagctgcga tcgaaagcga acgatcaatt gtccctgaac tggaggatat atacaagaga
     5041 aaggatctat tcgtaaaaaa cagccaatgg atgattggtg gagacggatg ggcttacgac
     5101 attggttttg gcgggatcga tcatgtactt gccagcggag cagacgtgaa tattcttgtt
     5161 ttggataacg aagtctattc caatactggc ggtcaaacct caaaagcaac accagcttcg
     5221 gcaatcgcta aattttcggc tagtggaaag tatgcttcaa agaaagatct aggaatgatg
     5281 gcgatgacat atggaaatgt ttatgtggcg caaattgctt caggtgccaa tcaaatgcag
     5341 acgataaagg cttttgaaga agcagaaaag tatcctggac cttctttaat cattgcctat
     5401 acgccttgta tcacacatgg tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa
     5461 gaagctgttt cttctggcta ttggtcatta tatcgatata accctgcaat ggtcgaaaaa
     5521 aacaagaacc caatgacttt ggactacaaa aaacctgatt tctctgctat gcctgatttc
     5581 atgaggaaac aagttcgctt ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct
     5641 ttatttgaaa agactatcca agacgctaaa acacgttttt acaactatgc tgcactgacg
     5701 ggagaattag aaaaaatcaa gaaacgactt gaaccagagg aatctatcga tgtacccgtt
     5761 aagactagaa aagaaaaaac gacggacctt gaagcagaag aaagaagagc tgccagagca
     5821 gaacgacgta aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa
     5881 tataatgaat aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata
     5941 cttgcaattt tcctgaaaat tatgcgtttt tctcaagaaa aatacgagca gatcagcctc
     6001 taaaattaaa acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta
     6061 tgtcatttca aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg
     6121 acttgttctc tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg
     6181 tatacaaact gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag
     6241 ctatttttat tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc
     6301 tgatgaacca agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa
     6361 tccggagagg actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac
     6421 agcaggaaga tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc
     6481 gaaaaatcgg tgcgctaatt actatcgaac ggcatactgg attagatgag tatattgaga
     6541 caggaattgc attagatgca gatatcactg gagagctgct gatcaatatc tttattccta
     6601 atacaccgct gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg
     6661 cctatcttcc tctatcagaa agtatgttga ttccaaaaga attcgggact cgacaccggg
     6721 cagctgtcgg tataagcgaa gtcagcgacg cgattaccat cgttgtctct gaagaaactg
     6781 gggatgtcag cattacatta gataatgaac tgatggctgg cttatcccag caagaatatt
     6841 tggcaatttt gcgtagagaa ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga
     6901 ttcaatcttt cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa
     6961 acggacaagc attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa
     7021 tgcaaatggc cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt
     7081 tactggggtg ccaatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga
     7141 aaatgcagtg actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa
     7201 cgaagataca cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca
     7261 tgaggttccg ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc
     7321 gactattaca gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat
     7381 tccttcaacg ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa
     7441 agtctctatc actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt
     7501 ggacgcttcg atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa
     7561 tgcagcaggg gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga
     7621 ttcgatcaag ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc
     7681 cggggtagaa agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag
     7741 cgatttgctt gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa
     7801 tcatcgaaca actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga
     7861 atcagttagt gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac
     7921 gagcagtcgg acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg
     7981 aacggaacca tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac
     8041 cacaagtaca gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc
     8101 ctcagaaaat taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac
     8161 ggcgtacgcg gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg
     8221 tgcggaggtt atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta
     8281 gggcgggata caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta
     8341 tcggtgggta tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg
     8401 acacgtttgc aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa
     8461 gataatggaa tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta
     8521 gaaattgaag cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga
     8581 ttgggaactg tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa
     8641 acgatcaacg gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact
     8701 tcaacaagtg ttaatcgttt gtttgccgat cttgaaacag atttttatac catgggcaca
     8761 aatccagacg ggttaaatat caatgatggc gtaggctcta cccatccaga acgtttagct
     8821 gagatggtcg ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt
     8881 attattgcgg tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt
     8941 gctaaatact tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg
     9001 agtaacttag gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa
     9061 gttggtgatc gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag
     9121 cagtctgggc acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga
     9181 attcagttac tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag
     9241 gtaacaatct atccgcaaaa attagtgaat atccgagtaa gtgataaaaa cggtgcgatg
     9301 gatgttccgg caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga
     9361 agaatcctag ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct
     9421 actgatgaga aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc
     9481 ggactggatt aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc
     9541 tgacactggt ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt
     9601 ttgtcaacgt cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg
     9661 actgtggtta gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc
     9721 ttttctaatc cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc
     9781 aggtttcctc gagtatgcgc accagtcact tcttcactga tgattccaat cactggtgtt
     9841 ccttcttcga tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc
     9901 tggatataac tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg
     9961 ccacgtccaa tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct
    10021 aaataatctt tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga
    10081 gtgatatcga atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat
    10141 aaagttagta cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt
    10201 ccagcatgca atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt
    10261 gtaatcgtaa gtgaagggta gccgagacgg ttgattttta ccagaacttg gcggctatct
    10321 gctgtttcgc cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg
    10381 ttatagccaa attcactggc taaatgaacc tcaacaggga tctgtgtcaa tttttccaga
    10441 gtctgtttac ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga
    10501 tcgctatcag acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt
    10561 acgtattttt gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata
    10621 taaaaaggat aggtaccttt ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa
    10681 cgttctacga tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc
    10741 atctcttcat cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg
    10801 ctggcaatca cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg
    10861 acgtaaataa catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg
    10921 acccgcaaag cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt
    10981 agatgagcga taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca
    11041 attttaagtt cttcaaagtt ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt
    11101 tgggaagtat gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta
    11161 tgaccaatac ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt
    11221 ctgccttgtg ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa
    11281 tcataaccac ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt
    11341 aatccaatca ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg
    11401 tatgaatgct tccagctgca ggaaaatcct tttgttttat ctttgcagat attttttgtg
    11461 gattttctgt agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc
    11521 tcgtcatctg tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctctttt
    11581 gttttttatc tggaaacatt taaaat
//
LOCUS       DAHEYX010000079        11599 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHEYX010000079 DAHEYX010000000
VERSION     DAHEYX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.080"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(164..2239)
                     /locus_tag="MB219_001447"
     CDS             complement(164..2239)
                     /locus_tag="MB219_001447"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCC8427169.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            complement(2241..3158)
                     /gene="glyQ"
                     /locus_tag="MB219_001448"
     CDS             complement(2241..3158)
                     /gene="glyQ"
                     /locus_tag="MB219_001448"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCC8427170.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3542..4354)
                     /gene="recO"
                     /locus_tag="MB219_001449"
     CDS             complement(3542..4354)
                     /gene="recO"
                     /locus_tag="MB219_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCC8427171.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4497..5396)
                     /gene="era"
                     /locus_tag="MB219_001450"
     CDS             complement(4497..5396)
                     /gene="era"
                     /locus_tag="MB219_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCC8427172.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5411..5806)
                     /locus_tag="MB219_001451"
     CDS             complement(5411..5806)
                     /locus_tag="MB219_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCC8427173.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5790..6266)
                     /gene="ybeY"
                     /locus_tag="MB219_001452"
     CDS             complement(5790..6266)
                     /gene="ybeY"
                     /locus_tag="MB219_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCC8427174.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6283..8475)
                     /locus_tag="MB219_001453"
     CDS             complement(6283..8475)
                     /locus_tag="MB219_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCC8427175.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8499..9470)
                     /locus_tag="MB219_001454"
     CDS             complement(8499..9470)
                     /locus_tag="MB219_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCC8427176.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10176..11555
                     /gene="brnQ"
                     /locus_tag="MB219_001455"
     CDS             10176..11555
                     /gene="brnQ"
                     /locus_tag="MB219_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCC8427177.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3142 a         2431 c         2114 g         3912 t
ORIGIN      
        1 ttttatttcc ttaattttca tagttgtttt taaaagttac acaaaaaata aaataacaaa
       61 aaaattaaca ttgaattaat aatagttatt tatatttata acaaataaac cagagaaata
      121 gcctattttg tgcctgttct ctggtttatt tacatattag aaattatttt gtgatgattt
      181 gatctaaact tgctagtgac aaagccatgg cagcaatttg atttaattgc gttaatcggt
      241 tcttacgtac tgctggttct tctgccatga ccatcgtctg atcaaaataa ttttcgatca
      301 gtgggcgaag gcttacaagc tgatcataat tttcgttgat cgtttggtca gcaaagcttg
      361 ttttcattgc ctctactgct ttgtgcagtt ctttctccgc ttcgttttcg aacagttcag
      421 ggtctacttc tgattgtttt tcaagctccg cttttttcgc taagttcacg acacgtgtca
      481 aagcttccat cgatggtttg aagtcttcat ctgcaagatg ctgcttgaac aattgtgctg
      541 ctgcaaatac ttttgtcaga tctttttgtt ctgcgttcaa aacagcttcg atgacatcat
      601 gacgaatatt cttcgttgtc aataattggc gcagacggcc tttgataaag tcgatcacct
      661 gttgttgtcc cgctgacaag ccaatgccaa aacgatccgt atctttactg ataacttcac
      721 taatcgtttc ttgcagaaca gacaaaggaa aggcccattt tttcgattcg atgattcgaa
      781 caacaccata tgcttgacgg cgcaaagcat atggatcgtt tgagccactt gggatcatcc
      841 caactgcaaa gaaagaaaag atactatcta atttatcagc aatcgctaaa acagaaccta
      901 ctgtagtctc aggtagatct ccttcactgg atataggcat gtaatgttca cggatcgctg
      961 ttgctacggc tggtttctcg cctttaagca gagcatattt ttctcccatg atcccttgta
     1021 gttcagggaa ttctcctacc atgttggtta ctagatcaaa tttatagatt tcagcagcgc
     1081 gttgcagatc catcaattct tcctcagata gtcctacttg ttcgcctatg atgcgagcga
     1141 tgacttgtac ccgttgcatt ttctcgtaga tgctaccgat tttttcatgg aaagtaacag
     1201 tttttaattt ttcaacgtat tggtcgattg aagctttctt gtcttcatta taaaagaatt
     1261 ccgcatcttc taaacgagca gtcaatactt tttgattgcc tcgtaccaca ttttccaatt
     1321 ttacccgatc gccattacga gcagaaataa aatgaggaag caacatacct tgatcgttcc
     1381 gtacttcgaa ataacgttga tgttctttca tagaagtgac tagtacttct tcaggaacag
     1441 atagatattt ttcttcgaag tcgccagaaa agacagttgg atattcgact agattgttga
     1501 cttcttctag taaatcttca tctagatcaa tgatccagtg atttttttct gctaattcgt
     1561 ttgcttgtgt gatgatcatt tcttttcttt tttgcggtga agcaataaca aattgttcat
     1621 ttaatttttc ttcgtattct tttgcatgcg taaatgtcac atcttcacct aagaaacgat
     1681 gacctcttga agctctgtct gttttgatat ccaagatctc aaacgggatt acttcgtcat
     1741 ctaacaatgc cacgatccag tgaatagggc ggatatattc aaaatcgtat tctgcccaat
     1801 gcatcgttac tgggaaagtg aggcttttaa tgacatctgc taaacctgtc aatacttctt
     1861 ttgcttcttt gccgtgagtg tatttcgtta catagacata ttccacgcct tttaattctc
     1921 taaaagtgat agcatctgtc gtcagacctt gtccgcggac aaatccttca gctgctttcg
     1981 tccagtttcc atctgcgtcc aaagcaattt ttttggctgg tcctttgact tcttcttcac
     2041 tgtctgcttg acgttctgga atgtttgtta ttttgattgc taagcggcgc ggtgtagaaa
     2101 acgtttcgat cgtatcataa gtcaattggt tttcatctaa aaattttgct gttttttctt
     2161 ctagttgttt catgcttggt gtgacaacat gagcaggcat ttcttccagc ccgatttcaa
     2221 gtaataagtc ttttgccatc ttattcagcc tccttcatgt tttctggatt tgctgtgttt
     2281 ttattcaata aagggaatcc taatttttcg cgttctgcca caaagatttt ggctacagca
     2341 cgagccatgt tgcggatacg ggaaagataa cctgcacgct ctgttacgga tactgcaccg
     2401 cgagcatcta ataagttgaa tgtatggctg catttcaaaa tataatcata cgctggatga
     2461 acaagatttt catcgataca gcgtttagct tctttttcaa aacggtcaaa attgcttagt
     2521 agcaagtctt ggtcgcttac ttcaaaagaa tatttggaat gttcatattc tggttgtttg
     2581 aagatctcgc catatttcac accatcagcc cattccagat catagacact ttcaacttct
     2641 tgaatatatg aagcaagacg ttctagtcca taagtaatct cagaagtaac tggtttacaa
     2701 ggtagtcctc cgacttgctg gaagtacgtg aattgtgtga tctccatccc atcaagccat
     2761 acttcccagc ccacaccggc acaacccatc gatgggttct cccagttgtc ttcaacaaaa
     2821 cgaatatcgt gttctaatgg atcaattcct aaaagacgca agctttctaa ataaagctct
     2881 tggatatttt caggtgaagg tttcattacg acttgaaatt gatgatgctg atacaaacgg
     2941 tttgggtttt cgccataacg cccgtcagct ggtcggcggg atggctctac ataagcagca
     3001 ttccatggtt ccggtccgat tgcccgtaag aatgtatatg ggctcatcgt acctgctcct
     3061 ttttctgtat cataagcttg catcagcatg cagccgtttg atgaccaaaa tttttgtaat
     3121 gttaaaatca tatcttggac ggtcaatttt ttactcatct gttttgcccc tttcaatttt
     3181 taagcgttgc tgtcaataag aatacaggaa agaaaacgac aaaaagtccc tatgtctgat
     3241 tacagacata gggacgaata atttcgcggt tccaccctac ttccgaataa agattcggca
     3301 gcttcattta tcgtactcgc aagtgcctct tcaaaaatcg tttgggtccg gcttccactc
     3361 tcccgaactc gctaaaccac gtgtttttta ctgatcttgg tcaatgtacg tattcatttt
     3421 gcagttttct tgtttatata atagatcctc tcagtgaacg atctatttaa tctacggtta
     3481 ataatagacg gaagtccctg tatttgtcaa atagttttga tttttttgtt gtatctttca
     3541 tttattcttc tttttcaggt atctcttttt caggcatatt tttctctggc gtctttaata
     3601 cgtgtgccca agacttcatt tgatcaatga actttttgct ttttagatga ataccgacat
     3661 attcatcata caactggtca atgaggtcac ggattgcctg cttcgtttct tctttcaagt
     3721 ggattccact gatcttatca taagaaatag ccgaaaacat tcgaacgaaa taaacagcgc
     3781 gaggatctgc atgataacgg tgttcgtcca ttggccagtg tttctcgcat atcacaccgc
     3841 catatttgga agaatagtca aattttcctt ttgtttcccc gcatacgaca cagtgcgtcc
     3901 aaattggcgc gattccgaag cgttgcagta tttgaacttc aaagatattc gtgatgattt
     3961 ctgcatcatc tccttgatcc atccgctgca gagcttgttg cgtaaattgg aataaatgcg
     4021 ggtcatattg atgatcttcg attgctgcat ctactagatt tagcaaatag gtaccataac
     4081 cgctcaaaaa gatatcttct tggatgttcc gaaacgcatg gacgtctttg acactgctta
     4141 aaaacgacaa accttcactt cgaaaattac cgatatagac tgcttcggta aaggggagca
     4201 aagccggagc taatggctga ttttgccgat ggattccttt gatataaaac atcattttgc
     4261 cagaagattc cgtaaagatt ttgactaatt tatctttttc tttgtaatta cggctagata
     4321 aaatcaagcc tttcgattct gcaagctggc tcatattctc cctcctaaaa aataaatctg
     4381 gaaaagcaga ttctgccgat ccagattatt tatgtttcga tcttccgccg tttttctcaa
     4441 cgtgcggatt tttatccagt ttatttctat acttaaccta taagaagttc agaatatcag
     4501 tattcgtctt ttcggtagcc aaagtcttgc agatagactt ttttgtctcg ccagtctttt
     4561 tggactttga cccaaagttc tagaaatact ttgtctccta aaagattttc gatatccttt
     4621 cttgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat gattcctttt
     4681 tggctatctc tttcgacgat gatcgtggcc tggatatgga ttttatcatt ctcgtttcgc
     4741 ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt taaaaacaag
     4801 actttctcac ggatcaattc agaaacgata aagtattcag gatgatcagt cacttgatca
     4861 tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat taagcgttcg
     4921 acattgtttc cttctgtagc agagatcgga atcacttgtg caaaaggcat ctgcgtcgaa
     4981 taatcttcga tgattcccag caactcgtct ggatggatct tatcgatttt attgataatc
     5041 agataaactg gagaatgcac gttttttaga cgttcaataa taaaatcgtc tccccggccg
     5101 cgtttttgat cagcactgat cataaataag actgcatcca cttctttcaa cgcactgtaa
     5161 gctgcttcta ccataaaatc tcctagacga tgctttggtt tatggattcc tggtgtatcg
     5221 ataaaaatca gctgagcttc tggtgtagta tatactcctt ggattttatt tctagttgtc
     5281 tgtgccttgt cactcataat ggcaattttt tgacccacga ttcggttcaa taatgtggat
     5341 tttcctacat tcgggcggcc gacgattgcg acaaaccctg atttatgttc tggcatatac
     5401 gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa atcaacccaa
     5461 tgatgacagc aaaacaagcg gtcactaaaa ctgctccagc tgccatatct ttgatttttt
     5521 tcccaatcgg atgaaaatga aaatccgtaa acatgtccac tacattttca aaaacagtat
     5581 tgatgatctc tactatccaa acgagaaaaa cagctaaaag cagccataac cactctatcc
     5641 gatcaagctg gaaaatcacg cccataaata aagcaaggat accgaacgca acatgtttcc
     5701 tcatattgcg ttcttcctca aaaacggttt tgactccttg gacagcaaat tccacagaca
     5761 tcaggaaatg tttgtttttc tctacgtttt tatcttttga gtccataggc atccaagatc
     5821 tctttctgta aaccaaacat ttctttctca tcttctggtg tcatatggtc atacccgttg
     5881 atatgcaaga atccatgtac cgctaaaaag ccaagttccc gatcatatga atggccgtaa
     5941 gcttctgctt gttcagcagc acgttcaata gaaatcatga tgtctcctag tacacgaggc
     6001 aatggattct ctccatcttc aaaaatcact gggatttcgt cttcgccttc ttcttccaga
     6061 gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt gattacttgg
     6121 attgccgcat tgtccatgaa agtaacagac atttctgttt cctctggcag ctttaaataa
     6181 gatgctgcaa attgcagcaa gtcatcgatt tctttcattt tctcttctga gacttggttt
     6241 gtttcatcca taaaggtaat atccattata catcgcttct ttctattctt cttgttcttc
     6301 ctttatcttc tcaacttctg ttttcatttt aggatatttg atccgagaat ggaaggtgct
     6361 acttaaaccg ctgatcaatg atttttccac ttttcggatt tctttcaagg tcagtccact
     6421 atcgtctaat tgaccatcgg aaatacgctc ttcgataaga ttatggacaa aactttcgat
     6481 tttttcgctc gtcggatgat ccattgcccg tactgcagcc tcacaactgt cagcaatatt
     6541 gacgatccct gcttcacgac tttgcggttt tggtccagga taacggaatt gctcttctgt
     6601 tacttccgga ttgcgttctt tcgctttgat gtagaaataa cgcattagcg tggttccatg
     6661 atgctgtttg caaatatcga tcaccatttg cggcatatta taatcttcca atattttggc
     6721 tccatctgtc acatgtccaa agataatatg tttactgtct tctggcaata agaaattgtg
     6781 cgggttttcc gctcctgaag gcaagttctc aacgaaaaag ctagcatgtt tgatcttgcc
     6841 gatatcatga taatagcaag ctactcgagt tagcaaagaa cgtccgccga tttctgctac
     6901 agcgttagca cttagattag ctaccatcat actatgatga taagtacctg gcgcttcttc
     6961 cagcaactgt ttcaataaag gatgatttgg attgctgagt tcatttaata caattacact
     7021 gtcatcattg accatcaatt caatatatgg gtgtagaccg atcgtcagca agaatgagaa
     7081 gagtgcacca gtgaacccgc agacaagcat taaccaagtc gttgcatcac caaagctcat
     7141 tccttgatag acgatcaaaa tgacatccat catcacagga aaaataatga cccacatggc
     7201 agcagataat ccttgttctg agatccgttt gcgtctgaca atcgttgcca tgagcccaga
     7261 gaaaacataa gacatcaaga taatcgttag aaagttcgtg ccaatcgaat cataaaaaat
     7321 gaaaagtgca gatactgctt ggaaaatacc agccatgatc cctgctcttc ggttcaagaa
     7381 aaagcttaaa acaagtggtg caaaagcagc gggataaaac agcgggatat aagtagcttg
     7441 ttccgtttgg aataattgga aaaatttcat cagcactatg ctgactgaca tagctgtcac
     7501 atagaaaagc acgtattttg ttctttctcc tgcattttgg aattgaagtg aattataaat
     7561 cagtacgccg atttgaagca aaacagctaa aaccatggca attagcggaa aaatcgaagt
     7621 tgtttgatta gtcagtccta gtaattccaa ttttttcata gctgaagcat cgatttgatt
     7681 accttcacga acaatgattt ctccttggaa gatcatcact ggttgtacag cttctcgtgc
     7741 ggattgtttc aattcttctg tctttttctc attcaatacg tcattcacta cgataccttg
     7801 atccactaag tagcgaatca tttgttgttg tgctggtgtg acattcaaat attggatttg
     7861 ctcttctgct tcttgtttaa aagcttccaa ttcactttca cgaacttgtt tgctcatctg
     7921 ttcgtcaatg agcttcagac tctcatccct cacagtatgg atctcagttt cagtcatttc
     7981 aaatatcgtc gaataaaaat tgttcggtag tttttgataa aacgcaacat tttctgcatt
     8041 cacattttca aaatttttct tcaacgcggc gatccgctct tctgtagtag gctttgggat
     8101 attttcatct tcctttgcct tatcaatctt ttcgtcataa gatttgttga tggcatcatt
     8161 cgttttatcg atcaattcaa atagttgctt gatccgattg ttctggtcat ctgcaagatc
     8221 tttttggtaa gtgtattctg gagtgactgc ttctgctgcc agttttcttt tttgttcggt
     8281 ttcttccgtg ttctcgatcg ttttgttcgc acggatactt tcttctgcta cttggccttc
     8341 tctgaaatca ttggcctttt gtttgacact actaaatgta acgccgcaga taatgataaa
     8401 aaacaagaac agcaggaaag gcaatgctgc ctttccaagt ttttcttgta gtttctgcag
     8461 tgttttattc aacatgtgct tatcctccta agcaaagctt attcttgatg gagatctgcc
     8521 ttttcataag ctttgatgat ttcggcaact acagggtgcc gaacaacgtc acttgcttca
     8581 aaattaacaa aatcgatttt cttgatttgc tttaatgttc gttctgcatg aacaagacca
     8641 cttgttgtcc ctcgaggcag atcaatctga ctcgtatctc cattgacaat catttttgat
     8701 tgataaccaa gacgtgtcag gaacattttc atctgagcaa ctgtcgtgtt ctgtgcttca
     8761 tccaaaataa cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa aggagcaatt
     8821 tcgatcacgc ctcgttccat caatcggttc gtatgatcta atccaaaaat ttggtataaa
     8881 gcatcataaa caggacgcag ataaggatct actttttctt ttagatcccc gggaagaaag
     8941 cctagatttt cccctgcttc cacagcaggc cgcgtcaaaa tgattttttg cacttcacct
     9001 tttttcaagg cagcgattgc taatactact gcaagaaaag tttttcctgt cccggctggt
     9061 cctacgccaa atacgatatc atgtttggct accgattcga catatttctt ttggccgctg
     9121 ttttttaccc gtattggttt tccattacgg tctttgacga tctcttcctc atacatttca
     9181 acaaaataat ctaatgtgcc tttttgcccc attttcagtg ccgtcacgac atctggtgta
     9241 cttacatgga ttcctcgttt gatcaattcc tgcaatgtcc gaatgactga ggcagccaag
     9301 gaaatctggg attgttcgcc aatgatctga atgatttctc cacgtgtatg gatggttgtt
     9361 tctgttgttt cttcaatcag ttttatatgc ttgtcgtgtg cacctaaaag catgctgaca
     9421 tcatcagcag ccgtcaatct aatctctaaa gaactagact cttccgtcaa aaatagacat
     9481 cccctttttt tcgattcatt ctttcctatt ttaccatact cagaagaaac tatatagcaa
     9541 ataagaaaag gttctaaaag agttgcttaa ccataagtac caagaaacgt taaaaaatag
     9601 ctgcttccat tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt aagctatgca
     9661 taagatagag gacaagtatt ccgtctaact tagcaagtat tctctgggta tttttcttgt
     9721 ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa acaggtgtat
     9781 tataacgtca acaaataaat atacagcaaa gcgtcgagga aaacagtagg ctattcagcg
     9841 atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca
     9901 tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg
     9961 ataactttat cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta
    10021 ccgttctaga atctagagcc cttttcccaa gtggaaaagg gctttttttt atattttaag
    10081 gtggtggttt tcatggaatc tgatattagt ggcatttcta gtttctgcaa cgtttatttt
    10141 gttactcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa acttattcat
    10201 ggaagcaatt attgatggtc agttcactta tcttcgggat gttcttcggg tcaggtaatc
    10261 tgatctttcc tgtccactta ggtcaaatgg ctggatcaaa ctggatcagc gctggtgtag
    10321 gatttgccat ctcaggcgct cttttcccat tattagctat cttagctgta gtagtgacaa
    10381 aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct gccttgttct
    10441 tgatccttgt tcatttaacg atcggaccat tctttggtac accgcggaca gcagcaactg
    10501 cttttgaaat ggctgctaaa ccctttttgc ctgaaaaata tgattcatta ggtatgtttt
    10561 tattctctgc tcttttcttt ttacttgctt atttccttac tgtacatcca acacgtttgg
    10621 tcaaatatgt cggaaagtat ttaaactcct tgtttcttgc attattagct atcatttttg
    10681 tcgtttcttt attccaaccc atggggaatc taaaccaatc tgctgcagat tcttatcaga
    10741 cgaacgcttt gacaaatggc gttttagaag gatacaacac ggttgatgca gtagctttac
    10801 ttgctttaag tgtcactttt gtccatgcag ttcgtgatct tggttttaaa gataaaaaag
    10861 tcacagaatt aacagcaaaa gctggtagcc tgagcgttgc gttagaagtc atgatttatt
    10921 ttggtctggt ccttctcggt gcgatgagtt tgaataagat gcctttatca gaaaatggcg
    10981 gaactgcact ttctcagatc actaattttt atttaggtaa cttcggtgct agcttcttag
    11041 gggtaatggt cacattaggt gtcttcacta ccgctatggg actcgttgtt tcttttgcac
    11101 aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg ttaacgactt
    11161 ttgtctcttt tgtcgttgcg aatgccggat tggataatat catccaatgg tctttaccag
    11221 tattgatgct tttgtatcca ttgtcacttg cgttaatctt gctttcctta acagcaaaat
    11281 tcttccaaaa gagtccattt gtctatcaag taacgatgtt cttttctgcc gttccagccg
    11341 tattggatat gttggcaaat tcgcctgcat tagtgagtca gcaaactgtc gtagcaagct
    11401 tactaggatt ttatcatcat cacgttcctt ttgctgcatt aggacttggc tggatggtcc
    11461 caacgttact tggttacgct ggcagcttac tcttttatta cgcctatcgc ctatcagggt
    11521 ataaacaaga agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa tttaacataa
    11581 gagatattaa acaggaaaa
//
LOCUS       DAHEYX010000080        11572 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHEYX010000080 DAHEYX010000000
VERSION     DAHEYX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.081"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            436..2076
                     /locus_tag="MB219_001456"
     CDS             436..2076
                     /locus_tag="MB219_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCC8427178.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2230..3552
                     /locus_tag="MB219_001457"
     CDS             2230..3552
                     /locus_tag="MB219_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCC8427179.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3614..4045)
                     /locus_tag="MB219_001458"
     CDS             complement(3614..4045)
                     /locus_tag="MB219_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427180.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(4109..4945)
                     /locus_tag="MB219_001459"
     CDS             complement(4109..4945)
                     /locus_tag="MB219_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCC8427181.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(5135..5437)
                     /locus_tag="MB219_001460"
     CDS             complement(5135..5437)
                     /locus_tag="MB219_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCC8427182.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5463..5894)
                     /gene="ruvX"
                     /locus_tag="MB219_001461"
     CDS             complement(5463..5894)
                     /gene="ruvX"
                     /locus_tag="MB219_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCC8427183.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5891..6157)
                     /locus_tag="MB219_001462"
     CDS             complement(5891..6157)
                     /locus_tag="MB219_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCC8427184.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6411..7136)
                     /locus_tag="MB219_001463"
     CDS             complement(6411..7136)
                     /locus_tag="MB219_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCC8427185.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7502..7572)
                     /locus_tag="MB219_001464"
     tRNA            complement(7502..7572)
                     /locus_tag="MB219_001464"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7538..7540),aa:Gly,seq:ccc)
     gene            7689..8441
                     /locus_tag="MB219_001465"
     CDS             7689..8441
                     /locus_tag="MB219_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCC8427186.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8457..8936
                     /locus_tag="MB219_001466"
     CDS             8457..8936
                     /locus_tag="MB219_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCC8427187.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(9187..10767)
                     /locus_tag="MB219_001467"
     CDS             complement(9187..10767)
                     /locus_tag="MB219_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCC8427188.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10760..11503)
                     /locus_tag="MB219_001468"
     CDS             complement(10760..11503)
                     /locus_tag="MB219_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427189.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
BASE COUNT         3532 a         2301 c         2060 g         3679 t
ORIGIN      
        1 caatctaacg atttttttac tgggaagtta tccagggaga agtatgctca aaatgcctta
       61 cacccaaaat ttattcctca atatttttga acagtgaatg tttgaagtaa ctttttagtc
      121 aaaatcagtt gatagggata ttagtgatac atatttcaat aaaatgtgcc aaagaattaa
      181 agctatgaat tattcaggat tctatttatc tatctaacat atcctttatt cttctacttt
      241 tctttcttta gcatataaga gatacgcata gtaatcaagc aaggattgtt gatataaaaa
      301 gaacaaatac cgctattttg atttcttttg actctattac tcatataaat aaaatttatt
      361 atacctgctc tttttgacta tatcctcatc tgactcctat acttcgtttg tattaaaact
      421 taggaggaat cattcatgct tacagggcta aacttattaa ataatccttt tctaaataaa
      481 ggaacagctt ttacaaaaga agaacgggca aaatacggaa tcactggtat gcttccaagt
      541 actgtccaaa cattggaaca acaatctgtc caagcttacg gtcaatactt aagcaaacag
      601 accgatttgg aaaaacgtat cttcttaatg aatctattca atacgaaccg gacattgttt
      661 tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt
      721 gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cacaagatgc tgtatttctt
      781 tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa acgcagcaga tggacgcgac
      841 attcgtttga tcgttgtgac agatgcagaa ggaatcttag gtatgggtga ctggggtgtg
      901 aacggtgttg acatcgctat cggtaaattg atggtctaca ctgccgcggc tggtatcaat
      961 cctgcacaag tcttgcctgt atcaatcgac gctggtacta ataacgaaga attattgaat
     1021 aatccattat acttagggaa tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc
     1081 attgatcaat tcgttcaatc agctactgaa ttattcccag aattgttatt acattgggaa
     1141 gattttggac gaggaaatgc tgcaacgatc ttagaaaaat acgaagataa aatcactact
     1201 ttcaatgatg atatccaagg cacaggaatc gttgtactag ctggtgtatt aggtggattg
     1261 aatatatcag gtgagcaatt gaaagatcaa acgatcttga catttggtgc tggtactgct
     1321 ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa
     1381 gaagcacgcc aacatttcta tcaagtagat aaacagggtt tattgtttga agatacaaaa
     1441 ggattgactc ctggtcaaat cccattcgct cgtaaacgct cagaattcac taatgctgat
     1501 gaattgacaa acttagaagc agttgtaaaa gcaatccatc caacgatcat gatcggtact
     1561 tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggcggc tcatacccca
     1621 cgtccaatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa agcaaaagat
     1681 ttgattgaat ggacggacgg aaaagcttta gttggaacag gtatccctgc tgctgatgtg
     1741 gaatataacg gtgtaactta ccaaatcggt caagcaaata atgcgttgat gtacccaggt
     1801 ttagggcttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat catttctcaa
     1861 gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc tatcttgcct
     1921 ccagtagcaa aaatcactga attctctcaa acgattgctg aaactgtcgc aaaatcagtt
     1981 gttgcacaaa aattgaatcg tgaagaaatc acggatatca aagaagctgt tgaatctgct
     2041 aaatgggttc ctgaatataa atctttagaa gattaagatt gttatagtaa aagaacatac
     2101 agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca
     2161 actactgctc ttttgccgct tctctttggc ttttttcata cgatacagaa taagaaagga
     2221 tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa
     2281 gggttttggt caacaaaaat taccggcgta agtttgcccc tttatttgat catggtcctt
     2341 gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga atatgattgg cccaatcgct
     2401 gttttggtca ttttcggtaa tttatttcat tttatcggaa caaaaatccc tatagtcaaa
     2461 agttatttag gtggtggctc cgtttttgcg atctttgcct ctgccgcgat tgctacattt
     2521 gggatcttac ctgagtatgt agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt
     2581 ttagactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa ccgtaatcta
     2641 ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt cgtctccttt
     2701 tttgccgtag gactcgtagg tatgcttatc ggaaatggtt ttgcaaattc cgttttatat
     2761 atctcattgc ccgcaatggc tggtggtgtt ggagccggag ctgtaccttt atcaacgatt
     2821 tatgcaaatg ttttaggtac ggacgcttct tctatcattt cccgcctgat tcctgcgaca
     2881 gcaatgacaa atgtcctagc tatcattggt gcagcgttag ttgctcgtgc gggacaatca
     2941 atgccaaaat tcaacggtaa tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa
     3001 cctagaaatg taaaaccttc cattccacaa ttaggtgtcg gtttgatggt atcattgact
     3061 ttcttcatct taggacaaat ctgtaactat tttgttccta aagtccatgc ttacgcattc
     3121 atgatcatta tcgttgttat ctgtaaaatc gcaaacctat taccagaata ttacgaagat
     3181 gcagctatca tgttcaacaa cttgattgtc aaaaacttga cagcagctgt tcttgccggt
     3241 atcggtattg cgttattgaa tctaaatgta ttggcttctg ctttgacatg gcaatttgtt
     3301 gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt caggatttgt cggtcgtttg
     3361 tttggtttat atccaatcga atcaactatt actgctggct tatgcaacaa ctcgatgggt
     3421 ggtacaggaa atgtagctgt cttatctgca gctaatcgga tggaacttat tgcttttgct
     3481 caaatgggca atcggcttgg tggtgccatc gttttgatca tctcaggttt cttgatgcaa
     3541 ttactttctt agtcaaaaaa aatacctgat catcaagtat cggcttgatg accaggtatt
     3601 ttttgataaa aatttacagg attttcaaaa atcttttttg cattttatcg gaatgataac
     3661 cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc tgcagaatta agtcttattg
     3721 ctgatattcc tcgtcttttc gcttcttcta cgccagtcat caaggccttt ccgattcctc
     3781 ttttttcagc atgtttagac acagcaagtg ccataatatt gaacatcgga tcgctgaaaa
     3841 tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta ggatcttcat
     3901 agaccaagaa aaaatttttt tctggctcct tcaacagttt cttcatttgt ctcaccgtaa
     3961 cggttaatgg tacatcatat cctaattgtt ccgcattgat ttctcgtagt gcttctgcat
     4021 ctcctaaacg catttcccgc aacatccaaa tcaccagcca atttttattt ttttaaatga
     4081 atttttttat cacagtatac tccttttttc acttaaaaaa agacttttca gtgattttga
     4141 cttgcttgaa tccagatcct tctaatgttt gcaagatgac gaaagcattg taaagattga
     4201 tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt cacttttttt ggagacaaaa
     4261 ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata acataaaaca
     4321 acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc aattttgcca
     4381 gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt ggcaatgcaa
     4441 acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa aaactagcta
     4501 aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa tgaaattttg
     4561 gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt
     4621 ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt
     4681 tgccgactac tcctccaaca acagctccac tagataccca accgacaatc tgccaattca
     4741 gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa
     4801 ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca agaatcggtt
     4861 tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc agaacgatca
     4921 ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat atcttttagc
     4981 gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt cgaaagcttt
     5041 tctacttttt aaggaaattt agtgctaaac gaaaaaaccg ggctctactt ttcagagagt
     5101 ccagcttttc ttacacctta tcgattcttt tgttctattc ttcttccgcc atgaatgtat
     5161 tgaatacttc ttcgatcata tcccattctg cgtctgtttc gatttgttct aactcgcctt
     5221 ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg
     5281 aaacaccagc tggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg
     5341 ttagcaagat ttcgtataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt
     5401 gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag attacctctt ttctttttat
     5461 tattaaaata attgcttacc tgagccgtct aaataatttt gtaaaatcat cacagctgct
     5521 aatttatcga tgactttttt tcgtttcgct cttgaagtat ttgcctgctc tactagcata
     5581 cgttctgcct gaacagtggt tagtcgttca tcttgataat caaccggcaa ggagaatagt
     5641 tctttgatct tctctccata tgacatggaa gcttctgccc ttggtccgat ggaattgttc
     5701 atattcttag gtaatccaac tacaaaacgg tctacttcgt actgcgccac taattccttc
     5761 aaccgatcaa aaccaaactc ttcttctgcc tcattgattc gaatgatttc tactccttga
     5821 gctgtccagc caaagggatc gctcacagca acgcctactg tcttagaacc gacatccaag
     5881 cccattactc tcataaattc actccatgat ttgagaggta gtctttgaca attacttcca
     5941 tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc atcacgataa cgaggaatgt
     6001 aggccgggtc tccagaaagc aagtagccga caatctgatt gattggattg tagccttttt
     6061 cttccaaagc ccgataaaca atcgctaatg tttcgctcac ttcttttttg cgactatcgt
     6121 caaaaccaaa acgtacggtt tcatctgtaa aacccatgat tctacacctc tttcccgcaa
     6181 ggtaattcca gcttaccttc tacaccctta ttttactcga agaaagggaa aactacaaac
     6241 aaatattcca aacttctgaa taaattaaga aacacgattt gtctgatttt tccttttttc
     6301 tgtggctttt tcaaaagccc tctttccata aagtatacgc tatatttctt tgaaagtatg
     6361 tttttacaca attttttcat aaagttcata aaaaattcga attgctgcta ctaatttttc
     6421 gttttcgttg cttgccctgt ctcataattg atggtgtagc catcttgaac attgtagata
     6481 aagacattga attcaatttt tttgtcttca atgctttctg cttccaactg cacacctctt
     6541 gctaccagtt catttccctc gaaatgagga gtgacacgat agcgtacatg atgatttgtt
     6601 tccttgatat atgccgcaat atcattttcg tgggcaagca tgtacggagc attcaacgaa
     6661 cgtgtccctg tcattaagtt tttaatgtta ttattttgac cagtcaactg gaaaccaatc
     6721 aaatggctgc ggttgtataa ccattgtccg tcacttaatt tcttttgttt ccaacctgtc
     6781 ggatcaatat acaaaggttc tctcttttca gtaggaaaca agtcttttcc caacatcgca
     6841 ttggcttgtc ctactctgtt caatttgtct atatttgaat agctttccca tgtccctttg
     6901 gaaagactaa gatcctcttt tgtaaactcc ggctgattcc cattgatttc tatttcttga
     6961 tgttttccat catattctgg cacatccgct acttgagaag cttgcggtgt ggaatcaaaa
     7021 atggttgtaa tttgttggaa gatatctgaa tctttttgtg tggtggaaat accgagtgcc
     7081 cctgcaatca cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct
     7141 ttactccttt actaacagtt ccctaaatct taacatatgt tcccctataa ataaatactg
     7201 gaaagaaata gaaacagtaa aaaaataaaa ccagttttta gacggcttaa gaatacaaaa
     7261 aaagagacgg attcatcgtc tctactcaat cacaaaacac tatttacaag gtatttatta
     7321 tcattcaaaa aatttttctt aatactcaaa actaaacggc ttctgtacga cctcttggta
     7381 aacagtgttc aaaagcttgc atctgcggta ataagtccaa ccaagtcaaa aacataagaa
     7441 gcttttttgc ttgactattc ttattacttg atgcagggca gctttttcac agcctctttt
     7501 aagcgggtga cgggaatcga acccgcgaca tcagcttggg aagctgggat tttaccacta
     7561 aactacaccc gcgatacaaa aacagtatac gcctctttca ctccttcctc aagttttttt
     7621 attctttacg cttccgatat gttttgtagt aaaataaaac taatcaatca attattggag
     7681 aagatgctat gacgggaaaa gaattagatc tgttgctgga cacttgttta ctcgctggga
     7741 aaatcatgat ggaaagcaat gcggagatgt atcgggtaga agatacgatg agtcgaattg
     7801 ctttagcttc tggaaatttc cgtttggtca gctatgtcac tcaaaccggt ctctttattg
     7861 gtcttgatcg tacctcgacc atccgaatgg aacaaattac caaccgttcg attaatttag
     7921 aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt tgcttcagag ctaacattag
     7981 aagaacttta ctgcgaactg caagcaatcg accgagaccg ttctttcttt cctgtctggt
     8041 tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat ggtgttattt ggcggaaaga
     8101 tgaccgattt acctgtgact ttagtcattg gaggattggg atacgcagct tatctatata
     8161 gtttgaaatt ttttagaatc aaatttttat cggaattttt atcttcttta ttgattggca
     8221 tagccgctat ctacagcgta cgttttggct taggaagcaa tcaggatcta atcattatcg
     8281 gatgtgtgat gcctttagtt cctggagttc aaattacgaa cgctatccgt gatttgctag
     8341 ctggacatta tgtctcagga gtttctcgcg gaacagaagc gatgatgact gctacgatga
     8401 tcggttttgc gattgctttt attttccagc ttttttatta ggaaaggaac acaagtatgg
     8461 ggacgatatt tattcagttt tcttttagtt ttttagcgac tgcggcattt gccgttatta
     8521 caaatgtacc taggcgttca ttgatttgtt gtggtctttc tggtgctttt ggctggatga
     8581 tctactggat ctgtgttcaa ataggcggat ctgctgcttt tggttcattg cttggttctt
     8641 taggagtagc attcatcagc gatcttttct ctaaacgttt gaaaatgccc gtaacgattt
     8701 tcaatattcc cgggatggtt cctctagtcc ctggtggttt agcttatcaa gcggttcgaa
     8761 atctcgttac tgaatcctat cgagaagcgg ctcattatgc ggtccaagca attatgattg
     8821 ctggagcaat cgctttaggt cttgtattat cagaagtctt taaccataac atccgtaatt
     8881 tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa acggcttttt aaataaacga
     8941 agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct tgagcgatga caaaatatct
     9001 cacaaacatt gcgacaagta accgcaggag caataagaag taattatgag tattaaaaaa
     9061 agttttgtaa aaatagcttc tcacattttt acataaaatt gacttaatac tgagaaactg
     9121 acttttgaac accctctatc tgtaaaaaag gggctgcgac atgatttttg tcacggcccc
     9181 tcttctttat tcaaattttt tccaaaacgt tcgttgataa taaacgatcc atagagcgga
     9241 aagtattaga actaaaccga tcacaggaat attcactaac caaaatggat aaaacaaagc
     9301 caacattccg tataagacga taagaataat actgatgatc aatgaaccca tcatagttgc
     9361 gactaaaccc aaacttcctg aacctctgtt aaataattgg ttaatattgg tccagttcag
     9421 cagcagcaac cgataatctc tagcgaaata acgtagtgac aataagtagc agccgaataa
     9481 agaaccaaat agcaagctag ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa
     9541 taatgcgatc ccaccagtta aaaaactctg caatatccat ccgaatctga acttttcttt
     9601 taaatattgg ttcatagaga ttggcaaaga acggataaac agaaaatttt cttgatccaa
     9661 agaaataaga ttgctgataa aagaagtctg attaactgtt aagaaagcca atgcgatccc
     9721 taccaggaaa acaacaccga ttaacttatt atctaaatca gttaatttta tttcgcctcc
     9781 tagggcaaaa gcaagaataa aaatgattgg agttaacaag gaagaagaaa gaacttgcat
     9841 aatcaaatta ggttctttta ctaattgagc attgtaacta aataaaagct gatgcacatt
     9901 ttggttcttt ttatgggttc tttttatttt gaactcactg tttgaatcag tcagttgctc
     9961 tgtaagcttt ggtactatca gctgttttac agcaaggaaa aatcccagag ccgtgatagc
    10021 tagtattccc caacccaata aaccttgttg agaaagtggc tgactggcag catcatagaa
    10081 tggcaataat atcgaaatcg gatgccgatc tgttaactca ttttcaatag tccccgtttg
    10141 atgattcatc cacatgattc cagcaaatgc gatcagcatc gagctgccaa gcaagatact
    10201 agaaactgtt tttctatgtt ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa
    10261 actagccaga caaaaaagaa taatcaaaaa tagagaaaac aaaaggattg agataatgat
    10321 agtaagcaaa atccctagat cagctcgcca tccggttaaa ataaacatga ctagaagcgg
    10381 aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc
    10441 ttgtccaaaa ggcagaggca aaaatacttg caggtcattt ccttcaaaaa agatattata
    10501 aatgactgat atactttgag ttaaacctaa cataccaaaa agtgccacgt aataagtaaa
    10561 gaaacctggc aatttaacaa agttcatagc aaacatagca aatccgtaaa tcagtaaaaa
    10621 aatcagccct gaaaaaagat tttgagacaa aagatagata gttaatcgct ttcccttttt
    10681 tcctttctct cttgctttat tcgtgagctg tggattaaca tataaaagat taactcgaat
    10741 taattctaat aattgtcgct tatacatacg gtccatctcc tgtcgcttgc cttccagcca
    10801 ttttcaaata aatcttttct agcgtttcat caggtgagcc attcaataat tcactaactg
    10861 ccccctgata aatcaattca cctttcttca aaatagccag tttatcgcat aactgttgtg
    10921 cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc ttttgcggca tgagccttca
    10981 tcatttcttt caaatcaaat gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata
    11041 cccaaatatc tggatctggc agcaacgtgc cgatcaaaat cgttttttga cgcattccat
    11101 gtgaaaaact tgcgattgtt tcattttgat gactatacat atcaaaaagc gctgtcaatt
    11161 ctgctaaacg cttttctttt ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc
    11221 aatattctcc agccgtcaat tgtaaaaaaa tatctgggga atcaggcaca taaccgattt
    11281 tttgtttgat actcaatcga ttttccgtca atttcattcc atctactaaa atcgttcccg
    11341 atgatggttc aataatacta actaatcttt taatcgtagt tgattttccg gcaccattat
    11401 gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag agacaagtct tttaatgctt
    11461 gttttttccc gtaactttta cttacattga tgaattcaat catttctgta ctccttttgt
    11521 ttgttattac tttttattgt acccttcgtt attggtggag taaatgtcaa aa
//
LOCUS       DAHEYX010000081        11489 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHEYX010000081 DAHEYX010000000
VERSION     DAHEYX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.082"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            35..3223
                     /gene="fss3"
                     /locus_tag="MB219_001469"
     CDS             35..3223
                     /gene="fss3"
                     /locus_tag="MB219_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCC8427190.1"
                     /translation="MLSLAVQSIVGAIAPTIAFADEITHPQTVTVELDLAHQYAVEGT
                     FSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKNPLPDMSDKAKLVS
                     VLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIADYQK
                     KPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVNGNQLIISPNANSK
                     ESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVEMEGSLKIKKVDKE
                     SGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDTT
                     PMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSLAGNTFAIRKDSPT
                     GEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELNYVNQT
                     VALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTLFTVKDGKAVKWTE
                     NFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYTLDETKYPVS
                     IKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGFNDLTFKVSPLEGT
                     NEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQKITPLEIRSTFK
                     ENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNRLMLYDLPEEEDSL
                     TSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDVDATKAAQEKDKKA
                     KPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYESKEAYEAGDEPVA
                     KDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFDDVSITHDVLDGSK
                     EAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDTSKYPEGTSFTFKE
                     INYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVPSGSQESSPTVKAF
                     PQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3335..3649
                     /locus_tag="MB219_001470"
     CDS             3335..3649
                     /locus_tag="MB219_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCC8427191.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3662..4036
                     /locus_tag="MB219_001471"
     CDS             3662..4036
                     /locus_tag="MB219_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCC8427192.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4037..4390
                     /locus_tag="MB219_001472"
     CDS             4037..4390
                     /locus_tag="MB219_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCC8427193.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4459..5475
                     /locus_tag="MB219_001473"
     CDS             4459..5475
                     /locus_tag="MB219_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCC8427194.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6069..7943
                     /gene="ltrA"
                     /locus_tag="MB219_001474"
     CDS             6069..7943
                     /gene="ltrA"
                     /locus_tag="MB219_001474"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCC8427195.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8069..8425
                     /locus_tag="MB219_001475"
     CDS             8069..8425
                     /locus_tag="MB219_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCC8427196.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            8470..9612
                     /gene="dcm"
                     /locus_tag="MB219_001476"
     CDS             8470..9612
                     /gene="dcm"
                     /locus_tag="MB219_001476"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HCC8427197.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9602..9891
                     /locus_tag="MB219_001477"
                     /pseudo
     CDS             9602..9891
                     /locus_tag="MB219_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10147..11331
                     /locus_tag="MB219_001478"
     CDS             10147..11331
                     /locus_tag="MB219_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCC8427198.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11328..11441
                     /locus_tag="MB219_001479"
     CDS             11328..11441
                     /locus_tag="MB219_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCC8427199.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3850 a         1973 c         2564 g         3102 t
ORIGIN      
        1 taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaatcaat
       61 tgtaggagca attgctccta cgattgcttt tgctgatgaa attacccatc cacaaacggt
      121 aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg
      181 tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcgtta aacaagatat
      241 tttctgtgtt gaaccaggag tcccgattta caatgagttc actcctggat atgagaagaa
      301 cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg
      361 tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta
      421 tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc
      481 aaaaatcaat caagccattg ccgattatca gaagaaacca agcttccata atagtacggc
      541 taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga
      601 gttcgatgag gttgtggaaa ataccgcaaa cattgattat cgtgtgaatg gcaatcagtt
      661 gataatcagc ccaaatgcca attcaaaaga aagtggtgtg ttgacgctta agaaatctgc
      721 tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc
      781 gatcgataag ccgaacactt atacggtcaa aattgatgtg gaaatggagg gttctttgaa
      841 gattaaaaaa gttgataaag aatcgggtga tattatacca ggaacggttt tccatttaga
      901 ctttggaaaa actttacccg caaaagacgt gaccaccgat aaagaaggca ttgcgacctt
      961 agatgagatt cctcatggta caaaggtaac cattactgaa aaatcggtgc cggcgcctta
     1021 tacgattgat accacaccca tgactgccac aattaaagca ggtgaaacca tttcagtgac
     1081 ttcaaaaaat acgcgagaaa aaggtcaaat tattctggat aaaaccggaa ttgaaacagg
     1141 gagtgatctt tggaacgata attactcttt agcgggcaat acgtttgcca ttcgcaagga
     1201 tagcccaact ggtgaaatcg tccaagaaat gaccacggat gaaaaaggtc atgcagaaac
     1261 accaaaagag attgccaatg cgttggaatt gggaacctac tatgtgactg aaaccaaggc
     1321 tagtcatggc tttgtgaata ctttcaaacc agtaaaagtc gaattgaatt atgtcaatca
     1381 aacagtggct cttgttacca gtaacgtaaa agggcaaaac caagaaatta ctggtgaaac
     1441 cactttgaca aaagaagaca aagataccgg tcatgaaact caagggaaag ctgagtttaa
     1501 aagagctgaa tacaccttgt ttactgttaa ggatggaaaa gcggtcaaat ggaccgagaa
     1561 ctttaaacca gaattgttga agggaacgaa agcttctgat gaaaccgtaa ctttgacttt
     1621 agatgaaaag aatcaagttg ccgttaaaca cttagcgatt aatgagtatt actggcaaga
     1681 aaccaaagcg cctgaaggat ataccttgga tgaaacaaag tatcctgtat ccatcaaaaa
     1741 ggtggatgat agcgaaaaaa atgctgtgat tactcgagat gttgcggcaa aagaacaagt
     1801 gattcgcttt ggctttgatt tctttaaatt tgctggatca actactggca ctgccgaaac
     1861 tggcttcaat gatctaacct ttaaagtgtc gccattggaa ggaaccaatg aaatcaccgg
     1921 tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa
     1981 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt
     2041 tcaaaagatt acgccattag aaatccgttc tacttttaaa gaaaataaag aggattatac
     2101 caagagtgat tatatcttta ctattaccga aaaagggcaa aagcaaccga ttaaaacggt
     2161 aacggtccct tatgggaatc taaccaatga gaatttctca gtcagtttga accgtttgat
     2221 gctttatgat ttgcctgagg aagaagatag cttaacttct cttgcgactt ggaaagaagg
     2281 agataaagaa ttgactactc ttgattctac cgagctagtc gataaattga gctataatct
     2341 acatgaaatc aaagaagact ggtatatcgt agctcaagcc atcgatgtag atgctacaaa
     2401 agctgcccaa gaaaaagata aaaaagccaa accggtggcg attgcggaaa caaccgcaac
     2461 tttagcgaat aaagagaaaa caggaacttg ggagattctg cataaattaa ctgtcgaaca
     2521 agttttagac aagaccattg tcttattcaa ctatgtgtat gaaagcaaag aagcctatga
     2581 agctggtgat gagccggtag cgaaggatgc tagcttgaac aatcaagcgc aaactgttaa
     2641 ttgtatggtg gaacgtcatg tttccatcca aaccaaagcg cacttagaag aaggttctca
     2701 gacctttact catggtgacg tggtagatat gtttgatgat gtatccatta cccatgatgt
     2761 actagatggc tcaaaagaag cttttgaaac aattctgtac gctttatttc cagatggtac
     2821 aagtaaagaa atttggaaat ctggcaagat tgattacgaa gtgaatgaca aagaatttac
     2881 caaaaccgta cttgcggaaa aagtggatac aagtaaatat ccagaaggta caagttttac
     2941 cttcaaagaa attaactatg acaaagatgg gaatatcaat ggaaaacaca atgaagattt
     3001 gaaagaaaaa tctcaaactt taacgcccaa aaaagttccg acaacaccaa gtacaccaga
     3061 acaatctgaa atgccaactg ttccaagtgg ctctcaagaa tctagtccta cagtgaaggc
     3121 attccctcaa accggggaga aaacttccaa tgtgctactg ttcattggct ttagcttgat
     3181 ctttgcgacg gctggctatt atttttggaa tcgtcggaac taaggtgata agatgaaaca
     3241 tgtaaatagt agaaaacaat acgcgcgacc gcctcctaaa aaagtggtcg cgctattttt
     3301 gtattcaaaa ctaacaatga aaagaggaaa aaatatggaa ttgaaatttg ttgtaccaga
     3361 tatggtcgag acgttcggaa aactaacata cggtggtgaa ggtgaagttc tatcggaagg
     3421 gtatggtcga aatactacaa ttgttggtcg aagttaccat ctgttttcaa ataaacaacg
     3481 ggccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt ttgaccaaga
     3541 tcaaccacta aaagctgtaa atccccactt aattgccaaa ggctatgaaa ttgagaatcg
     3601 tgggtttacc gattacgtgt tgtatgttga tgatttagtg aaagcatagg aggaaaagaa
     3661 gatgagatta acagaaggta ttgtcgtaga ttcaggatta acctttggca agttgcgttt
     3721 ttccgcatta cgtcgagaag tacgcaagca aaatgaggac ggaacggtta gtaacgaggt
     3781 aaaagaacgg acctataatt tgaaatcctc cgcgcaaggt cgaatgatcc aagtcagtat
     3841 tccagcaaat gtacccttac gcgagtttgc ttatgatgca gaggtagagc tggtcaatcc
     3901 aatcgtggat acggtcgcca attatgtttt tcgggaagga accaccgtta attggtttat
     3961 caaggcagat gatttagtct tgaaacgaca actgaatcaa ggaaaaccta cgaaccaaaa
     4021 tgaaggaaag aaataggtga agaatatgga aattaatgtt gaacatattt tagattgtct
     4081 cgatcaattt ggcaaaggtg agctaacggg ggaacaactc actaacgcat taacctatga
     4141 tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg atgccaaaga
     4201 agactttgat gtactggatt ggttagggga agaagagtct ttctttatgg ttgtcgaggt
     4261 aaatgaaagt ttatgtcggc aggcagaatc tgttttggaa gaaatcgatg tggagatgcc
     4321 ggatgccatc gaaagatttt taaaacaatt agtggaaacg aaacaacttc ccgtagtagt
     4381 aaatgactag cataactttt tgaaattgcc caatgaaatc ctattgggct ttttcgactg
     4441 gaggtggaaa ccatcaaaat gtacaaagga catcgcatac gcgcaggaga tcaacacttg
     4501 atttatcact ttgttcttgg ctggctaatc tttttgttca ttggttggat gagcgtcttt
     4561 tatttacaag aacttagaca atttgatatt tctaaggtat cattttccac tagtgaaatt
     4621 ggctggtcta tgaaggatgt aatctgcttg ctaggaagtc tcgctctttc aggaagcatg
     4681 atgttacttt atatacactt ttttcaggat cattggcgaa gtctttggca tcgacaaaag
     4741 ttagctcgga tgattctaga aaaccattgg tatgaagtaa agcagactca aggtgaaggt
     4801 tttttcaaag atttgaatag tagttggacc aaagagagta tcagttactt ccccaaaatt
     4861 tattatcgaa tgaaagatgg cttactttct attcgcgttc aaatttcact gggaaaatat
     4921 caggatcagc tattgaagtt ggaaaataag ttagaaagcg gactgtattg tgagttagta
     4981 gaaaaagaac tcaaagactc ctatgtggaa tacaccttgt tatacgatat gattgccaat
     5041 cgaattggga tagaagaaat agtggcagaa aatggggcgt tgcgattgat gaaaaatcaa
     5101 gtatgggcgt atgattcctt gccgcatatg ttaattgctg gcggcacagg tggtgggaag
     5161 acctatttct tactcaccat tattcaagca ctattgaagt cagatgcaga attgtttatc
     5221 ctcgatccca aaaatgcgga cctcgccgat ttaggtacga taatgcctca cgtatactcc
     5281 caaaaagaag aaatttctga atgtgtggaa gatttttacg aacgcatgat agctcgtagt
     5341 aggtcaatga aagaaatgcc caactacaaa acaggagaga attatgcgta tctcggactt
     5401 ccgcccaact ttttaatctt tgatgaatac gtggcttata tgggtgcgaa tcgttttcct
     5461 acaagcatcg aatagatgtg aaatggaagc ctattgagat tatcaatagt gacaactaat
     5521 gttttgagag atggaatgaa agccttcaag ttgagctgaa acatccccgc taatactcct
     5581 acatgctcta atcaatgaag tagattggtt ttggtatgaa gctaggtgaa gtcgtctgaa
     5641 atctgcctaa gtgcttgagc ataaggctga ctatagggcg ggcggctgaa aaagataact
     5701 atggtaagaa tgtctgaaaa tgactgacga atctgcgaat gtacgggtct aaaaggctga
     5761 tcttattgaa agataggaac atacggacgt atggtgagat accgacaagt aaggttccat
     5821 gatatgaaaa tcgggatagc taacgaagaa gctataattt cacaggctca tagtagatac
     5881 ctaaggttat acatgatagc taaaaacatt ggaacgatgt aagcagaaaa ctatcaatta
     5941 aatgactact tctagatagg tgcatataag taataacgaa attgctttgt ttctgtgaaa
     6001 gtaagggcac agtaccgatg aagcgtctaa caaatgtgga gggatagccc taagtctatt
     6061 taataacaat gaatgttgaa acagatgaag ttcaaggatc tggtaagaac gtgggaaaat
     6121 ccgttatgga ggtgccacgc ttgaacacaa aaatacgtta ctgggaatat tatgatctac
     6181 aagaaacatt cgatcagcta tttactcaaa gtagaaacgg gaaaaagttt tatcaattat
     6241 atgagttaat catttctgaa aataatattc ttttagcata ccgtactatc aaggcaaaca
     6301 aggggtcttc gacaccagga acggattcct tcacaattga taactataag gaaatgaacc
     6361 aagcagagtt tatccatctt attctcagtc atttagaaaa ttacaaacca aaatccataa
     6421 agcgagtaat gatacctaaa cccaatggtg agaaacgccc attaggaatt ccatgcatga
     6481 ttgatcgaat gatccaacag atgttcaagc agatactgga gccaatatgt gaagctaagt
     6541 tttatgaaca tagctacggt ttcagaccat tgagaagtgc aaagcatgct ctaggtcgca
     6601 ttatgtatct cataaacatc agtaaaatgc actatgcagt agacattgat atcaaaggtt
     6661 tctttgataa tgtaaaccac cgtttactga taaaacaatt atggaatata ggaatttgtg
     6721 acaaacaagt cttagctatt ctttcaaaat ctctgaaatc acccattcaa ggagaaggta
     6781 tcccgagcaa aggaacgatc caaggaggta tcatttctcc actattatct aatgttgttc
     6841 taaacgactt agaccattgg gtctcaaagc agtggcatac ctttgaaaca aagtatccct
     6901 atactaaagg ttacaataaa tttcgtgctc taagagatac aaatctaaag caaggctata
     6961 ttgtcaggta cgcagatgac tttaaaatca tgactaatga ttatccgaca gcattaaaat
     7021 ggttccacgc tgtcaagctt tacctaaaag acagacttaa attagatatt tccaatgaga
     7081 agtctaaaat cgtcaatttg cgtaaatgta agtcggaatt tttaggattc gctatttgcg
     7141 tgaaacaaaa agggaaaaaa tgggtttgta attcacgtat ttctaacaaa aaaaaggatc
     7201 agattaaaga agagattaaa caaagaatca aagatattca aaagagtcct acagcacaaa
     7261 atgctctcct gtttaatgct ctcgtgctcg gtgttcataa ttatttcagg tatgccactc
     7321 atattaattt agatttgaat aggatgaact acgttctttc attcactact tacaaccgat
     7381 tatcaaattg tagtacaaga tgttacccca acaaagcttc cccaacttac aaaaagtttt
     7441 actcaacaac gatgaaaacg tataaagtag ctggagtcta tttgtaccct ctttgcgacg
     7501 tatctacaaa aacaatttac ggatttaata cggaggatac accgtttact ccgtttggtc
     7561 gtcaaagatt ggagcacaaa agcctacagt ctcttgtgtt ccaagaatta agaaaattga
     7621 tggaatcgaa aataccgaat agaacggttg agtatttaga tagtagaata tctcgttact
     7681 caatgaaatc aggcaagtgt gagattacaa aacaattttt gccagcaaaa gccgttcact
     7741 gtcatcacta tttgccaaaa agtcttggtg gagacgacaa attcgataac ttgagaatta
     7801 ttcataagga tatccatctt ctgatccata caacaaataa aatgataatc gatcactacg
     7861 tgcatgaact gaaactggtg ccagagcaaa tagcaaaaat caacctttat cgaaagatgt
     7921 gtaatctgca aaacattcaa tagaaaataa atagatggaa cgccgtatga ggtgaaagtc
     7981 tcacgtacgg tgtggagcgg gggaaaagat ggagattata tcaaattctt acctatcgct
     8041 atagatgtta acgacaaaag aaagtgcggt gattttgaat aagcttaaac aaatcgtgat
     8101 gttaggccgt caatctggtt tctttctgat tctagcttgt caaaggccgg atgcgaaata
     8161 tctaggagat gggattcgcg atcagtttaa cttccgtgtg gctttgggtc gtatgagtga
     8221 gcttgggtat tccatgatgt ttggagaagt cgataaaaat ttttttatga aacgcatcaa
     8281 aggacgaggt tatgtagata cagggggcag tgtgattagt gagttttata cgccacttgt
     8341 accaaaaggg tatgatttct taagggaaat tagcaacatt gttggattaa ccgttcatac
     8401 ggaaagagaa aataatagtg tgtagcctag gaatatcaat aatcctgggc ttttttattg
     8461 aggtgattta tgacattttt agatttattc gccgggattg gtggctttcg cttaggtatg
     8521 gaacaagccg gtcaccagtg tatcggtttt tgtgaaattg atgaatttgc tcggaggagt
     8581 tataaagcaa tccatgatac aaggaaggag gtggaaatgc atgacatcac aagtgtatca
     8641 gacgaatttg ttcgatccct cggaccagtg gacatccttt gcggcggctt tccgtgccaa
     8701 gctttttcaa ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt
     8761 gaaatcgctc ggttcgccac tcttctccaa cctaagtttc tattccttga gaacgtccgg
     8821 ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac gttggatgga
     8881 ttggggtatg atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaagccgt
     8941 gagcggatct tccttatcgg acattctcga gatgcatgta ccgaacaagt atttcctatc
     9001 attggatcgt cttcaacatc tgatcaaaac atcagaaact tggtaaatat caatccttcc
     9061 aatcgaggaa tgggtggcca agtctatggt tcagatagcg tagcaccaac cttgaccggt
     9121 gatgaaggaa ttaaaattgc cttaccggta aaggatggga tttctgtagc gggtatgttg
     9181 tcgggaaatt ttgaacaagg caataggatt tatgaggtaa ccggaacagc ccccactttg
     9241 tcaacgaaac aagggggaac gaagattatg attcggaaaa atgaaacaga atatcaagag
     9301 gtgaagccag gggacagtgt gaacctggct tttcctaatt ccacaagtcg ccgaggtcgt
     9361 ctgggaaaac aaagtgtgca taccttacta acaggagacc aacaagcggt agtcacggat
     9421 cagtaccaga ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccagat
     9481 tgggcttttg atcgagcttc tcaagtcaat tcagatagtc agttgtataa acaggccgga
     9541 aactcagtaa cagttccggt catttttgat attgctagaa gattaaaggg ggtaaaagca
     9601 tatgatttat gacgagttga ttggagaaat ctactgggtg attggcaaga ttcaggcaga
     9661 tccgaaattg gaagaagaac tgtaccggct caattttgat attcgcaaaa acggggtgaa
     9721 agtccaggtg attcgtatgt agtggatgag gaaacggatg ctcgaatgga gctcaatcaa
     9781 gtcatcggtg aattggaacg aattgcggac cttgccaaag aacctgatat ccgccaatac
     9841 ttgtttgaga taaaagcaga gttggaaatt gagggtatca ccgcggagta atagggaaat
     9901 aacccactga taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg
     9961 tgcctgttca tggacggcgg cagccggcca tgatgtctca ccccccgtat ctaacagggg
    10021 ggtacaaaaa cacaaaaaac aagcaagtaa ccataaaaaa tgcttgttgt atcaaggata
    10081 ttaagtaatt tgtataggtg ttaactcatt tggaaaagtt aacaccttga atttggaggt
    10141 gaaaacttgg cgcaacgaaa tttagattat cggttattaa aagaacgacg caacgaatat
    10201 ggcgtttcac aaaataaatt agctactgct tgcggactta gccgcccata cctaaatcag
    10261 atagaaaacg gtggcgtaat cgcctctaca aagaccatga gaaaaatttt tgatcaactg
    10321 gaaagtttca atcccgattt acctttaacg ttactatttg actatgtaag gattcgcttt
    10381 cccacaacgg atgtgcggaa aattattcaa gaaattctcc atttgaaatt cgagtatatg
    10441 cttcatgaag attacgcctt ttactcctat caagaacaat atgtcatggg cgatattgta
    10501 gtgatgttat ctcatgaaga agataaaggt gtccttttgg aattaaaagg tcgtggttgc
    10561 cggcagtttg aaactttttt actcgcccaa aagcgtagct ggtacgactt ttttgaagat
    10621 tgtctaaaag ccggtggcgt gatgaaacgc ttggatttag caatcaatga tcgagtgggg
    10681 ctactggata ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt
    10741 cgtaccttca aaagttaccg ttccggggaa ctgttgaaag ctgatgaaaa ggatggtatg
    10801 ggcaatacct tatatattgg cagcctcaaa agcgaggttt atttttgttt gtacgaaaaa
    10861 gattatgaac aatatatcaa gttagggatt ccattggacc agaccgaaac gaaaaatcgt
    10921 tttgagattc ggttgaaaaa tgaccgtgct tattatgcga ttcaagattt gttaaaaggt
    10981 cgtagcattg aaagtaccac gttttccatt atcaatcggt atttgcgatt tgcggataaa
    11041 gtagatggaa aacggcgaac caattggccc ttgaatgaac aatggggtcg ttttattgga
    11101 cggaatcgga aagagattca attaacttct gaaccgaaac cgtataccat tgaacgaaca
    11161 ttaaattggc taggtagaca agtcgcccct acgtggaaaa tggctaagga gttggatcga
    11221 ttgaatcaaa caacgtatat ccaggatatg gtacagaatg cgcgactctc ggaccggcat
    11281 aaaaagattt tggagcaaca aagtatggca atcgaaaact tgattgtatg aaaagaggaa
    11341 ttttgatgga aattgtaaaa gatatgttat tggtacttgg tggcagtatg cttggtgtta
    11401 ccgtgatgtg cttgatgaat gcaagtgcag cagcggattg agcaatggaa aagaaaggaa
    11461 agtgacaaaa tgaatttcgg acaaaattt
//
LOCUS       DAHEYX010000082        11451 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHEYX010000082 DAHEYX010000000
VERSION     DAHEYX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.083"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="MB219_001480"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="MB219_001480"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCC8427200.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="MB219_001481"
     CDS             complement(2260..2511)
                     /locus_tag="MB219_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCC8427201.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="MB219_001482"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="MB219_001482"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCC8427202.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="MB219_001483"
     CDS             3461..3679
                     /locus_tag="MB219_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427203.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="MB219_001484"
     CDS             complement(3968..4480)
                     /locus_tag="MB219_001484"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCC8427204.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="MB219_001485"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="MB219_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCC8427205.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="MB219_001486"
     CDS             complement(6933..7376)
                     /locus_tag="MB219_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCC8427206.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="MB219_001487"
     CDS             complement(7377..8165)
                     /locus_tag="MB219_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8427207.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="MB219_001488"
     CDS             complement(8162..9103)
                     /locus_tag="MB219_001488"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCC8427208.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="MB219_001489"
     CDS             complement(9280..9867)
                     /locus_tag="MB219_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCC8427209.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="MB219_001490"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="MB219_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCC8427210.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         3111 a         2605 c         2019 g         3716 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc a
//
LOCUS       DAHEYX010000083        11336 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHEYX010000083 DAHEYX010000000
VERSION     DAHEYX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.084"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(35..970)
                     /gene="murB"
                     /locus_tag="MB219_001491"
     CDS             complement(35..970)
                     /gene="murB"
                     /locus_tag="MB219_001491"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCC8427211.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1043..1963)
                     /locus_tag="MB219_001492"
     CDS             complement(1043..1963)
                     /locus_tag="MB219_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8427212.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2076..2828
                     /gene="xth"
                     /locus_tag="MB219_001493"
     CDS             2076..2828
                     /gene="xth"
                     /locus_tag="MB219_001493"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCC8427213.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3814..4347
                     /locus_tag="MB219_001494"
     CDS             3814..4347
                     /locus_tag="MB219_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCC8427214.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4837..5361)
                     /locus_tag="MB219_001495"
     CDS             complement(4837..5361)
                     /locus_tag="MB219_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427215.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5354..5827)
                     /gene="tsaE"
                     /locus_tag="MB219_001496"
     CDS             complement(5354..5827)
                     /gene="tsaE"
                     /locus_tag="MB219_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCC8427216.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5950..6930)
                     /gene="pta"
                     /locus_tag="MB219_001497"
     CDS             complement(5950..6930)
                     /gene="pta"
                     /locus_tag="MB219_001497"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCC8427217.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7083..7763)
                     /locus_tag="MB219_001498"
     CDS             complement(7083..7763)
                     /locus_tag="MB219_001498"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCC8427218.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7889..8758
                     /locus_tag="MB219_001499"
     CDS             7889..8758
                     /locus_tag="MB219_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8427219.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8784..9299
                     /locus_tag="MB219_001500"
     CDS             8784..9299
                     /locus_tag="MB219_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427220.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9346..9939)
                     /locus_tag="MB219_001501"
     CDS             complement(9346..9939)
                     /locus_tag="MB219_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427221.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9964..10143)
                     /locus_tag="MB219_001502"
     CDS             complement(9964..10143)
                     /locus_tag="MB219_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427222.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10220..10729)
                     /locus_tag="MB219_001503"
     CDS             complement(10220..10729)
                     /locus_tag="MB219_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427223.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10825..11205)
                     /locus_tag="MB219_001504"
     CDS             complement(10825..11205)
                     /locus_tag="MB219_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCC8427224.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3252 a         2310 c         2023 g         3751 t
ORIGIN      
        1 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
       61 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      121 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      181 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      241 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      301 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      361 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      421 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      481 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      541 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      601 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      661 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      721 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      781 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      841 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      901 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
      961 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1021 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1081 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1141 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1201 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     1261 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1321 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1381 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1441 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1501 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1561 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1621 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1681 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1741 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1801 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1861 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1921 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     1981 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2041 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2101 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2161 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2221 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2281 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2341 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2401 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2461 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2521 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2581 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2641 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2701 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2761 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2821 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2881 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     2941 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3001 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3061 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3121 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3181 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3241 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3301 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3361 caaatggcaa aatagttcct catattttgc tattttttga gctaatacgg agggtcagct
     3421 gattgaacac tgtttagtgg agaggttgtg aaatcagcgt cctgacctga gtattagaga
     3481 aacaaatggc aaaatagttc ctcatatttt gctatttttt gagctaatac cgagggtcag
     3541 ctgattgaac accgtttagt ggagaagttg tgaaaaagtc gcctactttc gagtcgtaag
     3601 aaattttttt gaaaatggtt cctcacaact ttgcataaat ttgacttaat accgagaagt
     3661 catgatcccc atctaactcc aaatcacaag gaatgagttc aaaaactagc ttttctcaac
     3721 aaatattaca taaaaaattc tatgtttttt gttaaattta tttgcttttt tcaaaagtat
     3781 gttacagtaa tttcccaagg aggttttaca gaaatgaatt ttgttgccat ggattttgaa
     3841 actgccaatc atcagcctta tagtgcttgt tctcttgcgc tagtcatggt aaaaaatagc
     3901 cagatcgttg atgaattcta tacattgata cagcctgaga ctcctttttt ctggcgaaat
     3961 gtccaaattc acggaatcca tcaagaagac gtacgcaatg cgccgaaatt cccagaagtc
     4021 tggcaaacta tccaaccata ttttcaaaaa aatcgtttag tcgttgcaca taatgctgct
     4081 tttgatacaa aggtcttagc gggatgctta gattattatc atcttgaaca gcctaattat
     4141 ctttctttat gtacagtgaa aagtagccgt cgcctgtttc ctgaatttcc aaatcatcga
     4201 ttgaacactg tgtgcgaaaa tttgaacatc caattagatc atcaccatga tgcattggaa
     4261 gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa aaaaattcgg gacagatcca
     4321 ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg aaacacacaa agaggtcgtg
     4381 agcctgccgt ttcactccga gtcacaagga gcaatttcaa aaaatagctt ctcatgttta
     4441 ttaaatattg tgactggtga ccgaggagct ggcaggagaa caccgtttat tggagaggtt
     4501 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
     4561 ttttcatttg gttggactta ttaccgcaga tgcaagcttt tggacaccgt ttaccaagag
     4621 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca
     4681 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttattcaaa
     4741 aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc tgccttctca
     4801 aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat aactcatttt
     4861 atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga ccgcttcgat
     4921 ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt gaacgtcaag
     4981 ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt ctagcataag
     5041 agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt ctcccacgtg
     5101 agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg tcccttcata
     5161 gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact cttcttctaa
     5221 ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg tctccttatt
     5281 aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt cccgcaagat
     5341 gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc tgtttgaagg
     5401 tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa tctgtatcac
     5461 ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat agatttcccc
     5521 attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa tctgctccgc
     5581 tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga ctatattctc
     5641 taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct tgggcaattc
     5701 ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga ttgtcacctg
     5761 gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat cctgatagtt
     5821 cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg cgtaatgaag
     5881 tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg gctagttcgc
     5941 gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt tatatacatc
     6001 ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt gtagaattgg
     6061 tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat ttcctgactg
     6121 caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg gcgctttcag
     6181 ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga tcatgtattg
     6241 tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt ctggcgattt
     6301 cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat cgaacaattc
     6361 tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg gattcacgtt
     6421 gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca tgatcatcgc
     6481 tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg ggcgaacagt
     6541 gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca tatacgtaag
     6601 catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt tggatgcctt
     6661 tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt aattatcagg
     6721 gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat ttcttatttc
     6781 ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc gggcagctgc
     6841 gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac ggcgaacaat
     6901 tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt ttatatgtat
     6961 tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag tcaaaaatgc
     7021 tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa agcgctgttt
     7081 tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc aaggagcgca
     7141 tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga taacggactg
     7201 ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat tttttcttgt
     7261 gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa tttttcaatg
     7321 atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg cccttcccga
     7381 acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac tagatttcca
     7441 tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt tcttaacgat
     7501 ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg attttctcca
     7561 tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt caattccaat
     7621 gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc tttttttaaa
     7681 aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac ttcttgccaa
     7741 ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata actcacattt
     7801 tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt aaactaggaa
     7861 caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag atatggacgg
     7921 cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga tacgttttgc
     7981 aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag aagctcgcgc
     8041 agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg cacaggtatt
     8101 tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag caatctctgt
     8161 tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg atggtcttta
     8221 ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta cccatttacc
     8281 tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt tattgcacat
     8341 cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag ttttaaaaat
     8401 tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc agatttctct
     8461 tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga accataaaaa
     8521 cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt ctttagaaga
     8581 agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag caggagtcag
     8641 tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata caacggacac
     8701 aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag acttgtagtt
     8761 ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag cagcttggac
     8821 gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg atggttggtc
     8881 gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat ctcgttgctc
     8941 acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa gagttcgaaa
     9001 aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat attcgtagac
     9061 ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat catttcaatc
     9121 agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat gtattggatg
     9181 tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta gattactggc
     9241 tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac ttaagttaaa
     9301 aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga tattcttttg
     9361 catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga agtcatggcg
     9421 ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga tgacggagcg
     9481 attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga tgccttgtcc
     9541 tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa acgtttcttt
     9601 atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag gtaccagtgg
     9661 ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg caactttacc
     9721 gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat cagtgattgc
     9781 ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg gcagttccat
     9841 atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt ttctcgcaaa
     9901 acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga aatcgcaaaa
     9961 tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag taacgattct
    10021 tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat tatgttcgac
    10081 tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt tcattttttc
    10141 catgttatct caacttcctt tcatctttac tctcttagct tactcttata ttgtaaaaaa
    10201 gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt tctccatcta
    10261 ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac tctttcactt
    10321 ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt tgtttgaact
    10381 cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca tttcgtacgg
    10441 ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa aactggtatt
    10501 catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga atagcagata
    10561 tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat gagatccgat
    10621 attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc gtcataccgg
    10681 taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt cttcattctc
    10741 ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga atgaaacgaa
    10801 aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc cgtctggatc
    10861 aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt ctgattcgat
    10921 ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga aagcaagatg
    10981 acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa ttccaaaaat
    11041 ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt ctttgcggta
    11101 attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg atttctgata
    11161 atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag cactcctatt
    11221 cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg tcagattgaa
    11281 aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa agtagg
//
LOCUS       DAHEYX010000084        11323 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHEYX010000084 DAHEYX010000000
VERSION     DAHEYX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.085"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(279..494)
                     /locus_tag="MB219_001505"
     CDS             complement(279..494)
                     /locus_tag="MB219_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427225.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(546..1460)
                     /locus_tag="MB219_001506"
     CDS             complement(546..1460)
                     /locus_tag="MB219_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427226.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1460..2359)
                     /locus_tag="MB219_001507"
     CDS             complement(1460..2359)
                     /locus_tag="MB219_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCC8427227.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2359..2946)
                     /locus_tag="MB219_001508"
     CDS             complement(2359..2946)
                     /locus_tag="MB219_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427228.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3110..3301)
                     /locus_tag="MB219_001509"
     CDS             complement(3110..3301)
                     /locus_tag="MB219_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427229.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3374..4276)
                     /locus_tag="MB219_001510"
     CDS             complement(3374..4276)
                     /locus_tag="MB219_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427230.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4293..4769)
                     /locus_tag="MB219_001511"
     CDS             complement(4293..4769)
                     /locus_tag="MB219_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427231.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4785..5384)
                     /locus_tag="MB219_001512"
     CDS             complement(4785..5384)
                     /locus_tag="MB219_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCC8427232.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5388..5441)
                     /locus_tag="MB219_001513"
                     /pseudo
     CDS             complement(5388..5441)
                     /locus_tag="MB219_001513"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5943..6422)
                     /locus_tag="MB219_001514"
     CDS             complement(5943..6422)
                     /locus_tag="MB219_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427233.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6441..7070)
                     /locus_tag="MB219_001515"
     CDS             complement(6441..7070)
                     /locus_tag="MB219_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427234.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7067..8443)
                     /locus_tag="MB219_001516"
     CDS             complement(7067..8443)
                     /locus_tag="MB219_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCC8427235.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8523..9785)
                     /locus_tag="MB219_001517"
     CDS             complement(8523..9785)
                     /locus_tag="MB219_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427236.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9798..10145)
                     /locus_tag="MB219_001518"
     CDS             complement(9798..10145)
                     /locus_tag="MB219_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427237.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10165..11034)
                     /locus_tag="MB219_001519"
     CDS             complement(10165..11034)
                     /locus_tag="MB219_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCC8427238.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
BASE COUNT         3169 a         2283 c         1525 g         4346 t
ORIGIN      
        1 catctttaaa gaaatacgat caaaaaacat aattcccata ttatttgctc gttcaacaat
       61 ttgaaatata tctattagtt cttctttttt cataactgct cttcctttaa cttgttaatc
      121 taacttgctt acaaatatat cctaccacat ttgtgtacta tctgccaata tttttttgtg
      181 tataaatgtg tattataaaa caaataattt tacacaaaca aaaaagagcc taactgctct
      241 tagcaataag ctctcatcac tgctcctcgt gaatttagtc agtacaatgt atttgccttt
      301 tctttgataa aatccttata cgaaaaattg ttcaaccaaa attgttttag cactaccgaa
      361 tttttgtttc catcattcaa ctccaattga ccaaatctgc tgatcatatc atcaatttca
      421 tcaattatct cttgtgccat catgccgtct tctagacgtt ctaatacgta caattcaaaa
      481 acatcccttt tcattcaaaa tgcccctttc aggttacctt ttgccatttt atgggctaac
      541 tccgcctact cagctttttt tatataccta ctgatatttg ctttcgtatt tcttggcata
      601 ccacctgctt gcaggtaatg ttcacgataa taagcatcta gctctgaaac agctttttct
      661 aatctattcg ttgcattcaa cactgagcgg tattcacgtt tttcccttaa tacacgtctt
      721 aaatggttca tcgctgatat ataaggctta tagtattcca atacagtata tcctctagaa
      781 atataggcaa ttggatttct agcttcatgg atcaattcta aaatatctgt tggatcaggc
      841 ttatccggca tctcttcttc gatttccata atactgatca agtagtccaa tccggaatca
      901 tcgactccat aactttcaca ccattctttt aggttgttca agtcatgaat aacatcttca
      961 ggatcaaact ctctaaaagc agtgatccct tggctataat attttatata ttccattgcc
     1021 gattcaaaat cctctggcgg tatgaaagct tcaatttctt cttcatcaat cgctttcagc
     1081 actattccga tacctgagtt tgaaggatat tttctcaatc cttcttcaat taaatcctca
     1141 tctattcgtc ctttttgaaa catcaccaat aattgttcta acagctgatc taaaaccctg
     1201 cgctcttcat tcagttccat tgcattttta acttcttttt caccgagttc aaagtaagct
     1261 ttgatcaact tttcttgttc ttttcgttca actaatttct tcttcaagtc attcatattg
     1321 agaatgaaat ccatgtgatt actcttttgc tgattgacaa aatcatatct ttgttccaaa
     1381 acatcccgat atttttttag ttcttcttga attatttcaa agctatcaag tacatgaaga
     1441 attttttcat tattattcat cagcccatct cctgtagtct cacgtttgtt ttcgactttt
     1501 caaaagtatt gtatttaaaa gcaattaacc ctacaaccct gctatctttg atgcctattt
     1561 ccttgttaaa aattccgtcc aatgttcctt tgactaaatt atcaatatcc ggctgtgaag
     1621 tacgcatcag tgaatcaagc tttttcttat cttttctcac ttggtaaaaa taatctatct
     1681 gagctataac ccccatagga gcattcatta cctttttcag ttgatcattt accaaatctt
     1741 tctctttaag cgtattcttg atattttcca aatagtcgtt gtactctttt ggatagtaag
     1801 tgataatccc atttcctcta aatttagact ttggtcttgg cgttgctact ggtttaaaat
     1861 caaatgtagc ttcccatata tctccgttca atccttcaga agtatcccac tcaaattctg
     1921 aatatcgttt gatcctcaca actgtttttc cttcgttcgc cacattatat ttaaaagcaa
     1981 ttaagcctgt gatatggcta tctcgtatag ctgagatgtt aaaaatacca tccatagcac
     2041 tttttactaa attatctaaa tctggcgctg ttgtcttcat aatgttatag acttttttat
     2101 agctttttgg tatctggtag taaaagtcca cttccataat tgcgcccata ggatcattta
     2161 caatcgagta aaattcatca tctaataaat cttgatcttc gataaagctc tttatttttt
     2221 tcaaataatc aagatacttt ttatcatagt aggttgaaat ccttttctct gctacgtcat
     2281 tagtttcatc agcagcattc cttcttgcac ttggtcttgg tgcagataca ggttggaacg
     2341 gtaaagttac agtccacatt agtctttttt gatctctttc aaatagattt ttttaaaggt
     2401 atcaaggctt aaaggagcta ccttttgcat cttccggatt ttatttactt gttccaattg
     2461 tttcaataga ccatcttctt ttctaaggta ctcaggattt gttatttgtt tattccgata
     2521 ctcgattaat gattgaagct ctcttactgt taaaacatcc tcaatttttg cttctttact
     2581 cgttatcttt tttagatacc ctaatactgt tggataagat ttgccgatca cttctgcgat
     2641 ttctttttta ttgtactcac caaattttcc aagcaaccag tcattcatga tcttacttga
     2701 tacttttggg tatacaagaa ttgctttttc aatggaatga ctgccattat ttctgatcag
     2761 ttgactatct ttacactcaa ctgtaacgcc atcacctaaa tcaacaacat attgccaaac
     2821 gcctcttcga ttcttattaa gagaaataat cgttccctct ttatctctat aatcctttga
     2881 taattcactt actgttgtaa ctctttcccc aatgctaaaa tgtctaatat cattattgtt
     2941 ttccacttat tatccctcca atattgctgg ttatctcttc ctctgacaag gaactatata
     3001 tcatataaag aattttaaca ttattcggaa tgagtgtaaa gaaactttat ataaaaagag
     3061 tgatttagtc taagaccaaa tcactctttc ttttttatta attatattgt tatctgatag
     3121 acctattagt aaatgcacgt cttattgttt gatcattctc gatcaattct tctaacaact
     3181 ctgaaactgt tactttctta cctcttgctc tttcttcttc tttagctcgc ttttctaaat
     3241 atttaacaga agctattttt aaagtcacat actttctaac tttttcttta tttactacca
     3301 tcagctatca tctccaaggt gtcacaccta tatattattg gtgtgacacc atttgtttat
     3361 tataactttc tggctattta gccaatttaa tcgttaatgc ttcgtttgct gtagaatctc
     3421 caaaaacttt accgtcttca taaactacat gaactggcaa atctgcaccg tctgaatctg
     3481 gtttagcagt accacgatac aaaccaatta ctctttttcc tagttctctt gcaaccgtaa
     3541 cttttttatc ctgatccata gattcaactg aattgaataa ggtaacatta atcatttgct
     3601 tttcagtatc caatttaaca gttttgatag ctttgaagac ttcagtgtta aaccgatcag
     3661 ccatagaacc agaaacagat tcaaacgatt tgactttttc aggatcaaaa tcaattttat
     3721 ctggtagttc cgcttcgaca gtagaacttg attcagttgt actttcttct gtagctactt
     3781 cttttttcgc tgattcgata atctttttcg ttgatgtttt atttattttt acatctttat
     3841 agtattcttc tccatcaacg ttaaaaacca atgtcagctt gtgtacaggg ctattctctt
     3901 taactgtata aaacacttca aattgtgatt gagacggctt aatagtagcg agaacgtcgt
     3961 ctgatgcttt cttgccatct tccttcactg tcagcgaaac atttttataa ctcgttacat
     4021 tttcaaatgt tccatctatt actacccttc ccaaagaatc tggagcataa ctattattaa
     4081 ctcccgatat accaaattgt ttatcgttac ttccaaaaat cttagtggca ttaaacacaa
     4141 taaaggttac aatcacaatt ccaatgccta tgttacgcat aatcggatta tttgtttgaa
     4201 attgaccaat aattatagcg attgccccaa aaaaagcaag aattaaacat aaataaccta
     4261 acagggttaa tagcatgatt catcacaact ttctattctt tatttttctt tttggttttc
     4321 gtttgttcaa actcatgctc atttagataa gtaatcaata aatctcgcat ggatttgcta
     4381 tccttactta gcgtatcaaa cgcttcttgt aagtcagcat ccttctctac agcattaaac
     4441 caatgcttcg ttccaaattc aaaaagatta ctaatcactc ttaacggttc ttgatcagta
     4501 ttcgtattaa gccattcaag tatccgttgt tgagctaatc ctaatttact ttcgtcttcg
     4561 tgatttgtac gtgcttcata gattttagtg tgtatatcat caaaatagtg gattcgttct
     4621 cttagcgctt cacgttgttc gtacatagcc cttttatttc taacttcttc gtctattgtt
     4681 tcaatctttt ttcttaacag tatttgaagt gataacattt ctgagacaga caaaaacact
     4741 ggaattttct tttcttttct ttttggcatt tttagcactc ccttttaatt cttttgtttg
     4801 atcaatgaaa cgttctttag aataaatccc aagatcattc cacagacaaa tccgattaca
     4861 tgtgacaaga catttgcctc gcctgattca aaagtagata ccaataaaat gccgattatc
     4921 aataatacaa aaacacttag taataaatgt ttcttgctat caaaacggat gtatgcaatc
     4981 gcaaataaag caatccatcc aaaaatacct ccactaagcc ccatacttac gatgttgctg
     5041 tgtagcagaa aaggaactaa gcttcctaga aatcctgata tgattaaaac gatcaataat
     5101 gttaaccact catactgatc attcagtgct tcttcaagaa ctactaagaa caataaatta
     5161 gaaattaaat ggtacaagtt aatatgagaa acgatagaaa tgaatatatt cgggaacaag
     5221 tgtccttcat acaaaccacc taaagcaacc atttcctttg cactcaatgt atttccaatt
     5281 atgctttttc caatctgcaa aaaatcaaac aggaaaatca atacacaaac aaaaactaca
     5341 gtcgctacta ggtatcgact actatttttg tttttttctc tcattcgcta cttccttcgc
     5401 tttagtaact gccgattctc catattctct aaaaagagtc gatgttagct ttcctctcgg
     5461 aatattgtat ttttttaaaa tttcagttgt tgaaagattt ttttcaaagt cttcaataat
     5521 ttgtttatga agatttattt tagccaaatc attggttgtc aaaacaggtt ctttagcttg
     5581 aatatcgcct tgacgaatta acctttgaat tttatttttg ctttttaaaa aaggatattt
     5641 ttcaattaaa gggtcaattg cattcccttc atcaatccac ttacctattt cttgataaac
     5701 tctcttttga aattttttcc tcagctcaat cgggttcgga aaaacattgt gcttaaaatc
     5761 gtgtgatact aggctctttg atttgcctaa ttcatcagcg acttcttgca ttgtaatttt
     5821 gttttcgata tagcattgga caagagcatt atatttttta gggttattat tatttattgt
     5881 catgttctta cctacgttca ttgctaattt tttggtattt ttctgtttgt gtattgcgat
     5941 ttttaaaagg catttcctaa tacgttaaat tttgataatc ggttcaattt tactgcttta
     6001 tcaatttctg cctgtgacca aggttctatt aaacgcattt ggtacgtcac tagcagatcg
     6061 acatgttcat attcaaactt ttctattccg taaatctgat aaaatatttt ttcttcgttg
     6121 ttactataag gaatcactgt tccgatctta aatatgtttc gtgtgttgtt tacaccaatt
     6181 tcatcagtaa tcttgtgtct atattctaat aacacgtatt tcttatacct gcttgtcaga
     6241 tcaggtgaat agttaacaga ttgacaaacc aagtcactgg ttatacgtgg atcacctatt
     6301 ctgttaattc tcgtgttata gattccgata ataatatagt ttttatcttt atattctata
     6361 gtttctccca cttctaactt ttgattaaag cctgttctat ttgcttcaaa aagttctagc
     6421 aatctttttc acctctactg ctactgccaa acaaatttag ctttatcatg ccatgtttca
     6481 gacattactt ttttaccatt ttcgtcaaat atcaccgctt cccactttgc tgttccatcc
     6541 ggctgtttac aaatatctgc aaaccatagg atcgtttttc gatcgttgaa tttccttact
     6601 ttcttctcaa atttattgat ctgaactcta ttttgattgc tgttccaacc tactaatagg
     6661 cggttatctg attctgtacc atgaaaacca atgggaacga tgtgggttct atcaaatgct
     6721 tcttttcctt ctggatagag cattggtcta agtttcctac gtttcaagtc atctcttgct
     6781 tgataccgcc atgccgattt tggattgaat ttaatagcta tttgatgctt gcttttgtca
     6841 tggtaaaaat atgcgacatc ggcttcagct ccaagcaact tttctcttgc actatttgat
     6901 tcaaacggtt tattcacttc taaaaccttc acccctaaaa tgccatgatc aagcatctgt
     6961 ttaggagtta gtaccgcttt tttatctctt ttcttcgctc gtttcacaac tacttcatct
     7021 gtatatttat atggagtaag ccatcttaaa aaagaactga cgatgatcat ttatgcacct
     7081 gtttttttgc ttttaacatt ttttgcatga taggtgaagt acttccattt atttgtttct
     7141 tttcagattt atcagttgtg atcactacgt tttctgagcc ttttggatca ttagctttag
     7201 gtttggtttt agatgcaatc ggctgtttat tattcgtgtt gtttgaggtt tgcggttcag
     7261 gatcagcttt tcctaatgcc cttaaaactt tttctagttt ttctgcttgc agtttttcta
     7321 gtgttttagc tccctgctcg actttttctt ctttgttctt ttcaagctta atagcaaacg
     7381 cactcttctt ttcaattgat ggctctttag atgatggatt tgctaaaggt gtgttcaaat
     7441 ttcttttagc agattttctt acaggagctt taccaattcc accaaaatcc tcgtcttcac
     7501 cgccctcacc acgagtttca gctacgattg ctcggtaatc aacatattct ggttctggca
     7561 tagtatttct aaggtgattg aagattgttt caatttcttt gttactgatg taagctccct
     7621 gcgctctaac catacttcca tcaacaagct taataagcat atctcccttt ggacttagac
     7681 tttctccacc gccttcatct aatacaatcc tactttctaa atcacttgct accataaggc
     7741 aaacacgtga cggaaaattg gctttgataa taccagtgac aactgttgat cgaggagatt
     7801 gtgttgctaa gataacatga atacctgctg cccttgccat ttgtcctaat tgaacaatca
     7861 aaccttctac ttctttatgt gttccaacca actgtgaata ttcatcaact aataagatca
     7921 catagggaaa tctatctaat ccattttttt cggcatattc gttatatagc gtgatgtttt
     7981 gtaaccctgc tttttccatc agtttgtaac gttcatgcat caagatcaca agatattcaa
     8041 aaaaatcata agcttcatcc atatctgtaa ttggatcagc caacatgaac ggcaattttt
     8101 tgtatcttcc aaattcaacc ctttttgggt ctacaattcc tatttttaat tcatcaggcg
     8161 ttgagtgaca cattactgta acaagaattt cattcagtaa tactgatttc cctgatcctg
     8221 tcgttcctgc aaccaacatg tgtggcattt cgctgatgca aaaatgagca attgtgccat
     8281 caggcttaat tccaaaaata gcatctgttg ggtctttagc ctttgtgttc aacatattga
     8341 cactgaattt tccattaagc tttatattag gttctttatc tagcaaaata gattcagctt
     8401 tttcggctct ctttttgact ttctctaata atttcatagc catttctatt cctcactttt
     8461 ctcggtactt ttctgtttgt gaactggtag tttgtatttt ctgtttctat ttgtgatgtc
     8521 ttctaaaaca cgctattgat cattgatggt acatcaattg gaatacgatc attttctcct
     8581 aatggtaaag taatttgaat acgccctgcg ttgatccgca cgattacacc tgatatatgc
     8641 aagtaagttt caatttcttc tttcattgag cttagttgaa tttttctggt acgagataaa
     8701 gtaaacaaat attcaataga tgtattccca acgtgcatat ctgttagact tgcaggtacg
     8761 tcagcagaag atagataaag ttctaaagat tgcttttgag tttcggcata ttttctagct
     8821 ttttcggttt ggttttggat atttttggaa ttatcggcaa agagattgat tggaaaagaa
     8881 aattgtgaat ttgaaactgc gattgaagat gttgctcttt ccgtttcttt gtttttggta
     8941 cttactcttt ccttacgttc tctttcaata tttactgatg ctcggaaaat gaaataatcg
     9001 cctgaaactt catcttcttt ttcaccaaat ttggctcgtc cgcttgttgc tcttgtcagg
     9061 atcgacccta aatcttgaat ttgtcgtaaa actgtttctc taactttctt cccttttggt
     9121 tgcgggattt ttacgatata ttctcgaata tcttcattga cacttagatc acgtcttgta
     9181 tgaacagaaa ttttgatatt tttaagcgtt gatagcacat cggattctaa ttgcgttttg
     9241 tttcgttttc tttttttgtt gttcgtttca tctcctataa tcttgtttgg atcatacttc
     9301 acatccatag cttctagagc ttctcttttc aaccttctac tttccatatc atcaaaaaat
     9361 ggtctttgtt ctccatagtg atgcctgaac atcaaagtaa ccaaatatcc aacaaagaat
     9421 accgttcctg cttctaatac taaaatagag atgtctacca agtgtagctt tgcaaaaaag
     9481 ctttgtacat tatatgaata acttaaataa ttgggaaaaa acgcattgag taactcttta
     9541 aacatctctt ttaatcctaa atcatctgtt ccgttaacat catttaacga gacaagaata
     9601 cttactaagg tatctacaag cagttgtaca acctttgtta cccaatgcaa aatatatatg
     9661 ccaattgcac caactaccaa tgcacgtcca accagatata ctgatttaga aaaactggct
     9721 acaggataat atttttctcc atcgcttcgc ttatattcct ttaattcaat ggatttttcg
     9781 ctcatctgaa tatctcctta ttttctttct gattttggtt catcgtcact tatgctgtcc
     9841 tcacacatct gcgtatcttt aggaatgatt actctgattt cgtctaacat ttctctctca
     9901 atgatcatcg ccccttttgt actaacagtt ttctctgata attcaacaca tttccctatt
     9961 tcagaatctg gatcaaaaat agcctgttct acgtcaaaat gattcttagc tacatacacg
    10021 atactcattg tttctaactc tctagcaact gaatatttac ccttcttcag aagatagtag
    10081 ccatttcctt tttttattcg atttgcaact gatttatcta atccattgat gcttattgtt
    10141 tccaattctt caccttctca atttctaatc tcttacccaa gtaccatttt gatagttgat
    10201 gttgattcct ttttgattgt taaatacata aacattaagg tttaccgttt ttccattatc
    10261 gttaactgag aaagcttcga catgagaacc acgaggaact aattcattcc ctttataaat
    10321 tggatcaact ttatacaata ctttttcatt cttattttta tagattgcat cacgtacaat
    10381 cgtttctgag taagccatcc ctccattctg atcatttttc ccaacatttt gcgctctcgt
    10441 tccaagtgtt aaattgtgta cctccatatc tcctcctagc gaccatgcta atgaatgact
    10501 tttgttatag agataacctt tatagccttc tagctgagtg atttcattgt ttgaatcttt
    10561 gccgacccaa gtatctctct cttgatcaaa tactccatcc ttgtattctc ctgcaatatg
    10621 gacagaactt ttgaaagatg gacgttccac atttttagat gagtgagaat ttacagcatc
    10681 ataggtgatc aacgcttcaa catctcctgc tcttccaagt tgatctaaat ctgcataatt
    10741 tacccaaaag gtttgactgc ctttttctga taatgcttca tggtcttctt ttgtcagtgt
    10801 atatttccca tcattaatgt ttacgatatt atttggatat ttgtttggat cataacgtat
    10861 ccctaaaaat tcttttgatt cactgtctgc tttattacct acctgaacac tacttttgat
    10921 ctgaaaatca tgttcctgag tactgttatt ctgaaagtaa cccttgccac taagaccaac
    10981 cgcaactaaa accaaaattc ctaacaacga tcggctaaac ttcttctttt tcataccacc
    11041 attcctttta ttgtgttatc ataataagat aaatatataa taaactatat tgtaaatttt
    11101 gtcaaatgta tttttattta ttcccactat aaaccttgat atatagccaa tttaaaaaag
    11161 gatggcaaaa aattatcaag ttaaaaaaat aaattttgaa ccaaattatt tttaataaaa
    11221 tagctagttt ttttatcaaa aaaaatagag cttttcagct ctattcagac tgtagacaaa
    11281 ctcctaaatt tggagtgttg tttgcagttt tttgttttat act
//
LOCUS       DAHEYX010000085        11310 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHEYX010000085 DAHEYX010000000
VERSION     DAHEYX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.086"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1030)
                     /locus_tag="MB219_001520"
     CDS             complement(<1..1030)
                     /locus_tag="MB219_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8427239.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="MB219_001521"
     CDS             complement(1112..1801)
                     /locus_tag="MB219_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCC8427240.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="MB219_001522"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="MB219_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCC8427241.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="MB219_001523"
     CDS             complement(2328..2831)
                     /locus_tag="MB219_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCC8427242.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(2951..3078)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3157..3846)
                     /gene="rplA"
                     /locus_tag="MB219_001524"
     CDS             complement(3157..3846)
                     /gene="rplA"
                     /locus_tag="MB219_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCC8427243.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(3950..4372)
                     /gene="rplK"
                     /locus_tag="MB219_001525"
     CDS             complement(3950..4372)
                     /gene="rplK"
                     /locus_tag="MB219_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCC8427244.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4715..5383
                     /gene="sdaAB"
                     /locus_tag="MB219_001526"
     CDS             4715..5383
                     /gene="sdaAB"
                     /locus_tag="MB219_001526"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCC8427245.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5396..6268
                     /gene="sdaAA"
                     /locus_tag="MB219_001527"
     CDS             5396..6268
                     /gene="sdaAA"
                     /locus_tag="MB219_001527"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCC8427246.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6359..7378)
                     /locus_tag="MB219_001528"
     CDS             complement(6359..7378)
                     /locus_tag="MB219_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCC8427247.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7551..8456)
                     /locus_tag="MB219_001529"
     CDS             complement(7551..8456)
                     /locus_tag="MB219_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCC8427248.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8515..9060)
                     /gene="nusG"
                     /locus_tag="MB219_001530"
     CDS             complement(8515..9060)
                     /gene="nusG"
                     /locus_tag="MB219_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCC8427249.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9161..9331)
                     /gene="secE"
                     /locus_tag="MB219_001531"
     CDS             complement(9161..9331)
                     /gene="secE"
                     /locus_tag="MB219_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCC8427250.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9355..9507)
                     /gene="rpmG"
                     /locus_tag="MB219_001532"
     CDS             complement(9355..9507)
                     /gene="rpmG"
                     /locus_tag="MB219_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCC8427251.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9669..10313
                     /locus_tag="MB219_001533"
     CDS             9669..10313
                     /locus_tag="MB219_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCC8427252.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10369..10872)
                     /locus_tag="MB219_001534"
     CDS             complement(10369..10872)
                     /locus_tag="MB219_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCC8427253.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10885..10931)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3296 a         2346 c         2073 g         3595 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tgttcatcat ctcttcactt
     2941 cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga
     3001 atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg
     3061 tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta
     3121 tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg
     3181 gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc
     3241 tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc
     3301 atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta
     3361 agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc
     3421 agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt
     3481 agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt
     3541 ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt
     3601 gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac
     3661 agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc
     3721 aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac
     3781 aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt
     3841 agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata
     3901 tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag
     3961 tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg
     4021 cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg
     4081 caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata
     4141 gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa
     4201 taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga
     4261 atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg
     4321 ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata
     4381 cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc
     4441 tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca
     4501 aatttatttg ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc
     4561 taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt
     4621 gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac
     4681 aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt
     4741 ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc
     4801 tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta
     4861 tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg
     4921 cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga aattggcata
     4981 tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc
     5041 tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg
     5101 cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac
     5161 tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat
     5221 actttctgag accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga
     5281 aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact
     5341 caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt
     5401 tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat
     5461 gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga
     5521 aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt
     5581 cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt
     5641 tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa
     5701 tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg
     5761 tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt
     5821 atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga
     5881 agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg
     5941 tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat
     6001 gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa
     6061 tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag
     6121 tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc
     6181 gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc
     6241 ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt
     6301 agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt
     6361 aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg
     6421 tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta
     6481 gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag
     6541 ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt
     6601 tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca tctaattgac
     6661 gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg attactgcat
     6721 cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt
     6781 tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc
     6841 cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg
     6901 gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg
     6961 ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc
     7021 tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg
     7081 cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta
     7141 ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg
     7201 cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt
     7261 cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt
     7321 tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc
     7381 gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat
     7441 tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat
     7501 actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc
     7561 caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag
     7621 gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca
     7681 tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt
     7741 tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa
     7801 tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc
     7861 gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt
     7921 ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg
     7981 gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa
     8041 gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg
     8101 accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt
     8161 cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc
     8221 cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt
     8281 ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt
     8341 atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca
     8401 gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag
     8461 aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc
     8521 gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat
     8581 gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc
     8641 aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg
     8701 caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg
     8761 tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac
     8821 ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg
     8881 aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac
     8941 acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac
     9001 tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat
     9061 tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt
     9121 cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa
     9181 ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt
     9241 cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga
     9301 tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt
     9361 cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg
     9421 ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca
     9481 agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta
     9541 tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt
     9601 gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag
     9661 gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag
     9721 agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc
     9781 cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc
     9841 gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta
     9901 caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc
     9961 cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt
    10021 tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg
    10081 cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca
    10141 gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga
    10201 tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga
    10261 aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa
    10321 gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt
    10381 ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat
    10441 aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac
    10501 taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg
    10561 catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt
    10621 ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat
    10681 gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact
    10741 ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc
    10801 tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt
    10861 cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga
    10921 accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc
    10981 ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg
    11041 atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg
    11101 atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga
    11161 gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa
    11221 ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt
    11281 ttcggtcttc ggaccttttt cattttccta
//
LOCUS       DAHEYX010000086        11179 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHEYX010000086 DAHEYX010000000
VERSION     DAHEYX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.087"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(57..1100)
                     /locus_tag="MB219_001535"
     CDS             complement(57..1100)
                     /locus_tag="MB219_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCC8427254.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(1090..1581)
                     /gene="coaD"
                     /locus_tag="MB219_001536"
     CDS             complement(1090..1581)
                     /gene="coaD"
                     /locus_tag="MB219_001536"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCC8427255.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKNL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(1578..2138)
                     /gene="rsmD"
                     /locus_tag="MB219_001537"
     CDS             complement(1578..2138)
                     /gene="rsmD"
                     /locus_tag="MB219_001537"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCC8427256.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(2257..2595)
                     /locus_tag="MB219_001538"
     CDS             complement(2257..2595)
                     /locus_tag="MB219_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCC8427257.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(2579..3016)
                     /locus_tag="MB219_001539"
     CDS             complement(2579..3016)
                     /locus_tag="MB219_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCC8427258.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(3006..4130)
                     /locus_tag="MB219_001540"
     CDS             complement(3006..4130)
                     /locus_tag="MB219_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427259.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(4151..7579)
                     /locus_tag="MB219_001541"
     CDS             complement(4151..7579)
                     /locus_tag="MB219_001541"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCC8427260.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(7605..8768)
                     /locus_tag="MB219_001542"
     CDS             complement(7605..8768)
                     /locus_tag="MB219_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCC8427261.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(8771..9073)
                     /locus_tag="MB219_001543"
     CDS             complement(8771..9073)
                     /locus_tag="MB219_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCC8427262.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(9187..10959)
                     /gene="recQ"
                     /locus_tag="MB219_001544"
     CDS             complement(9187..10959)
                     /gene="recQ"
                     /locus_tag="MB219_001544"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCC8427263.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         3056 a         2444 c         1911 g         3768 t
ORIGIN      
        1 gaaaaagagg ggttgtgaca gaactcgtgt cacaacccct ctttaattat ttagtttcat
       61 ttcattttct ctagatagtc aagcgcttct tgtactgttt ttactggtac gattttcatt
      121 gaggaattca attttttagc ggcagctttt gcttcttcgt agttggtttt tatttcagga
      181 tattctttct taacatcgtc tgaaatcgta tcatctggtg caaagaaaat ctctacgcca
      241 gctttatccg cacttgccac ttttttatcg attcctccga ttcggccaac ttctccttca
      301 ctgttgatcg ttccagttcc tgcaatttgc ttaccatgtc gcaagttttt accagtgact
      361 tgttcataga tttctaaagt gaacatcaat cccgctgatg gcccaccgat agagccagaa
      421 ttaatacgaa catctgtatc tgattcgatc tcggtatggt cagtaagcgt gatacctatt
      481 cctgcctttt tatctgttgg taactcgatc agatcaccag aagctgtttt ttcttcacca
      541 ttatgcaaat aactgatttc gattgtttgt ccaacttttt ggctttttac ataatccata
      601 aatgcttctg tactttgaaa gcttttcccg tctacttttg ttactgtatc tccaactgaa
      661 agcttgccaa agaaattcga tgttttctct acactcatga catatacgcc tttgaattcc
      721 attttataag gcttgtctgc aagttttaac gcttgttcga ttgctgcatt ttgggaagaa
      781 tccatataat actgttgcat ttgattatat tcttcatttg tcgccccacc agtcaattct
      841 tcttcactga tcaattcttc aaaaggggtc attttagctt ttaaagctaa gaaacctgta
      901 gcttgcctga taccaacaga ggtaagagag aaagacccag aacctttatc ttccttatca
      961 ttgaccgtaa ttagttcttt cagatcgatc gtggctcccg gtgattctac ataatacggc
     1021 aagggaacta ctacaaatcc aatgatcaac attccaaata tgataatcag tactgttttc
     1081 aaccattgtt tagctttcat ttgattcacg ctttccagct aaagcttcgt tgatcactgg
     1141 cggtaagtag ctgctcacat ctccgccaaa gtgcagtact tcttttaaaa tactggaact
     1201 cacgtgacta tattctggtt ttgctaataa gaagacagtt tccaattcat catatagatg
     1261 atgattcatc tgagcaatct ctcgttcata ttcaaagtct ttgatacttc gtatgcctct
     1321 gatcaacgca tctgcaccaa tctcttttgc tgtttctacc gtcaattgat tttcttgatg
     1381 catgactttt acattaggaa gatgagaaac agcttttgtg atcaatgtca ttctttcttc
     1441 tgggggaaac aaatttttct ttgaagtatt gataaaaaca ccaattacta cctcatcgaa
     1501 taatttagcc gcacgttcca ccgtatctaa gtgacctttt gtaaaaggat caaaacttcc
     1561 tgggaataat gcacgtttca ttgatttccc tcttttctat agatagttac ggccgttatg
     1621 ccataaatct gttttcttat ttgatggaat ccagccacag tttctggcaa ttcgactgtt
     1681 ttatccgttt cgcatacgat aactgcggtt ttagaaaaaa gtcccagttc ttccatttta
     1741 tggatttgat cgattatttt ttgttttgca tatggcggat caagaaacaa gtagtcgaat
     1801 tgttgtttct ccgctgctag ccgttcgatt gctttgtccg catcaagttt tattgtggta
     1861 aatttctctg gttctttcgt aatttcgatg ttttctttga tgatttttag cgcttggtaa
     1921 tttttgtcta cgcataccgc atgactgcag cctctagata ctgcttcgat agctaaacca
     1981 ccgcttcctg caaacagatc caatactgtc tctccatcaa aatatggtcc aatcatattg
     2041 aatatggatt cttttacttt atctgtagtt ggtctggtgt tcgcccctgc taaacttttt
     2101 aaacggcgcc cgccataatt tcctgatatt atccgcatct actcactcct tagaaatggt
     2161 actggtacta gtataccaaa aaaatacttg gacaatgatc aaaaatcgat cgttatccaa
     2221 gttatttttt atttaaaaac tgctcataag agatttttaa agatgttcag ctaatcggat
     2281 ttctgtattt agctcttcaa tttcgaaacc ttcgtctttt aatactgttt tgtttcctat
     2341 tttttcggca aaattcattt cgatatccgg tcggtaagaa cgttctacgt ttcgaacaaa
     2401 atgtaattta cggattcgtt cttctgtttc ttccaatgtc tcttcattta agtaaagcac
     2461 gacatatttc attcttcttg agacataatg caacaagccg aactttttca gattctttaa
     2521 ttgttttaag ctgtagaccc atacgatcag cccgcgtcgt tttgttattt caaattcact
     2581 attgtctaat tgcatgacaa ttacctccgc atccaaagtg ttttttcgtt tcaaaaaacg
     2641 ggctacctgc atctactttg atttcattag aaacttcttc ggcaagtact tggacaattt
     2701 gatccaacag actttgcagc tgtgtttcac tatatcggaa agctgcaaca tgctcatcca
     2761 tatcaagctg tctttttgct tttcgaacag ctcgtttttt ttcacgataa tcaggtgcat
     2821 actcaccata tgccgctaca ttttcaaatg cttctttctt agacaagaac gcttctttca
     2881 actggcaaac ttttgcagat tcttccattg actgtttatg ttcaagataa gaagccatcg
     2941 ttttactctt ttttatcatt tcagcgactt ggaggcattg atcctccagt tgaaacagct
     3001 cttcgttaac gatcactgct atcactactt tctccgtttt cttcttgcaa taagactaac
     3061 atattttctt tagaaaaagc aatgcctaca gaatccgttc ccgggtccat gaaacggtca
     3121 tggttgtaag gatcgctgta ccaatataaa aacgaaaaag tagcgtcaat cgccggatga
     3181 ataaatagcc cactagtatt ttctaatttt tgtttactga ttaacttctc caattcatca
     3241 ttcgtcagtg agaatttcac tgtcgtttga gcactgccta gcgatttttg ccaatcagcc
     3301 atacgttcct ctgacaatat ttcctcaggc cttgtcaaaa aatcatgtag tagcagctga
     3361 ctgtggtttt gaaaggcaag tgtttgattg tatatgggaa gttcgtcttc tcttctaagc
     3421 atatttaata aagtcgtact ttttcgttct ttaacgcgat tgattgattc ccaatcagcc
     3481 gatttatctg cttgataatg tggaaggcct tcaccaaaca cttgatatgg caatagtttc
     3541 aaaagactat ctttatctaa atacacaact gaaaataggc cgtttgttgt ttgatcaaaa
     3601 aataaaatag caaaagtgtc gttatcaaaa gcaattagcg gacgataatt catatcctct
     3661 tccattagtt cgattccaac ttctgtatca tggtactcaa aagtgaaatt agtatagata
     3721 gtagtgattt tcgtaagatc ttgcatcgtc atccctaaat aaaacggcgc gatatcagca
     3781 ggccctgttt gcagtacttt aacagttgtg actctgctgt cttggatgtt cgcttcaaat
     3841 gtgagatgac tagctttatt ttcataatat cttgtttcaa aaccaaatcc gctagttaat
     3901 cgatcatagg cttggccaaa atccttttcc agctgatcga tcggttttcc gatataagta
     3961 gaaaaaccag ttgctttaag ctcctggtat ttccacgttg tcatttggtt tgatatttgt
     4021 tgatcagagt tttccttcac ttcaagtttt ggcggaaaca agactggttc catgtagaat
     4081 gccacaataa ctaatagcag aatcagccaa aacaaaccca gtcttttcat gtttccccac
     4141 ctttcgctta ttatttctca gtcacttcaa tcaaaaggtc accagacgaa atagtgtctc
     4201 ctgaagacac atatacatgt gcgacttctc cgtcaaaacg tgcttcgatg gccgtttcca
     4261 ttttcatcgc ttcagtaatc atcaacgtat cacctttttt aacgttatcg ccttttttca
     4321 ctaatacatc caaaacagaa ccagacatcg ttgctccaat ttgttctcgg tttgtcggtt
     4381 cagctttcaa tcgggtttgg accgtactga tgatcgaatt atctttgata gtgatttcac
     4441 gtctttgtcc attcaaattg aagaacagta cgcgattccc ttcgatatcg ggttctccga
     4501 tttcatctaa acgaatgata agcgtttttc cgcgttcgat ttgtacattg attgtttcac
     4561 ccaaacgtat accttggaaa aatgttggtg tgtccagtaa tgtaacatcg ccaaattgat
     4621 tatatgcttt ttgatagtcc aaaaagactt gcggatacat caaatagctt aatacttcgt
     4681 cttgttttgg ttcatagcca attttttctg ccaattcttt ttttacttcg ttgaagtcga
     4741 ctgattcagc caaaagacct ggacgttcat tcaatgcagg tctgccttta agaataatct
     4801 tttgcaattt tttaggaaat ccgccaacgg gttgccctag ttctccttgg aagaaagtca
     4861 caacagattc agggaaactt agtgtttcgc ctttttcata aatatcttct tctgtaagat
     4921 cattttggac cataaatagt gccatatctc caactacttt agatgatgga gttactttaa
     4981 cgatatcgcc aaacatcaag ttgacagtat gatacatttg tttgatttca tcccagcgat
     5041 gacctaatcc gacagctttt gcctgttgct gtagattcga atactgtcct cctggcattt
     5101 catgcatata tacttctgtt tcaggagcat tcaagccgtt ttcaaacggt ttgtaataca
     5161 tgcgcacatc ttcccaataa tggtttaatt tctgtgcatt ttcaatcgtg atttctggca
     5221 gccgtggacc attgaccagt gcataataca agctgctcat gctaggctga ctagtattcc
     5281 cactcattgc actcattgcc acgtcaacga tgtctactcc tgcttttgaa gcagcagaat
     5341 aagtgatgat tccattcccg ctggtatcat gagtatgcag atggattggc agatctgtcg
     5401 tttctttcaa ttcactaatc aaacgataag ctgcctgcgg tttaagcagc ccagccatat
     5461 ctttgattgc aatgatatgc gcacccagtt gttctagttc ctttgccatg tctttataat
     5521 actgaacatt gtattttgcc cgacttggat cgttgatatc gccagtatag caaatagctg
     5581 cctctgcgat tttaccagta tcccgtacag cttggatact tttttccatt tgtggagtcc
     5641 agttcaaact atcaaagatt cgaaatacat cgattccttg cgctgctgct tctttgacaa
     5701 attccaccaa tacattgtca ggataattag aatacccaac ggcatttgaa cctctgaaaa
     5761 gcatttgcag caacgtatta ggcattaaag aacgaatttt tctcaaccgt tgccaaggat
     5821 cttcattcaa aaagcggtaa gccacatcaa atgttgctcc cccccacatc tcacttgaaa
     5881 agagttcagg aagaccttct ccagtcaaac gtgcaatctg cttgaaatcc tttgtccgaa
     5941 cacgagttgc tagcagactt tgatgggcgt ctctaaatgt cgtatccgtc aacaacaaat
     6001 tttcctgtcc tttgatccat tcgaccaaag catcggcacc cttcgcatct aatacattct
     6061 tagccgttac gtaatcagtt cttgtgatta gatctttagg catacgtggt tcttcataaa
     6121 atggtttttc gccactttcg atacctggaa aaccattcac agtaatttca ccgatatatt
     6181 tcatcgtttt gtttccgcgg tctcgtagtc gaggaaattc aaataatgtt gctgtactat
     6241 caatgaaggt tgtttttgca tttcctgatt ggaattcagg atgtgtgatg acattgagca
     6301 taaatggaat gtttgttttc actccacgga ttctaaattc tttcaagcaa cgttccattt
     6361 tctgaatggc tgtttcaaag gtagcaccat gcgtacatac ttttactagc aatgaatcaa
     6421 aatacggtgt cactacatag cccgcgtaag catttccgac atccagacgt acgccaaaac
     6481 caccaggtga acgatacgta tcgattttcc ctgtgtctgg taaaaagtta ttcaacgggt
     6541 cttctgtagt gattcggcat tgaatggctg aaccatttaa tttaattcct tcttgctgag
     6601 gcaatccgat ttctttatgc agatccaaac cttgagcaat caacagttgc gttgttacga
     6661 tatcaatatc tgtaatcatt tctgtgatcg tatgttccac ttggacgcga gggttgactt
     6721 cgataaagta aaaatcatct ccttcaacaa gaaattctac tgtacctgcg ttgacatacc
     6781 cgacatattt catcaattgg acagctgcct ggcagatttt ttgacgttgc tgttcattca
     6841 tagagacaca aggcgctact tctactactt tttggtgacg gcgctgaaca gaacaatctc
     6901 tttcaaaaag atggatgacg tttccatgcg tatccccgag aatctgtact tcgatatgct
     6961 taggattagc gatgtatttt tcaacataca cttcatcact accaaaggct gcttttgctt
     7021 cgcttttcgc acgttcgtag ccctctcttg cactcttttc atcgtgagct acacgcatgc
     7081 cgcgtccgcc gccgcctaga gctgccttga tcataatcgg ataaccgtat tgtttcgcaa
     7141 actccaaggc atcgtctata gaagcgattg gtccatctgt gcctggaatc gaagcaatcc
     7201 ccgcttcgat agctgcagct tttgctttga ttttatctcc aaaaatatct aaatgatgca
     7261 gtttgggtcc aacaaaaatg atgccttctt cttcgcaccg ttgcgcaaaa ttcaagtttt
     7321 ccgacaaaag accgtaaccc ggatgaatag cgtcagctcc gcattctttc gcgatagaaa
     7381 tgatcccttc aatatctaga tacgcatcaa tcggtttttt tccttgtcct actaaatatg
     7441 cttcatccgc tttgaaacgg tgtacggaat attcatcttc ttctgcatag attccaacag
     7501 ttttaattcc taattctgta caagcacgaa atacacgtac agcgatttca cccctgttag
     7561 caactaaaac ttttttcatc gaaccactcc tacatctttt tttattataa acctaagctc
     7621 ttgcgttttt catccgcact gatgttgagg acaaagccga cacaaatcga aagcatcagc
     7681 aaactggagc ctccttgact taaaaatggg aacgtgatcc ctgtcaaagg aataacgcca
     7741 gtaattcctc caagattgac aaatacttgg atcaaaaaca ttgcgccgat cccaatgcac
     7801 aaaagcgaat tgaacggatc ttttgaacgg atacctacaa ggacaatgcg cacaatcata
     7861 aagaatagca acgctaatat caaaatcccc ataatcactc ccagttcttc cactacgatt
     7921 gcataaataa aatcggtttg tgcttcttgt aaaaatcctt ttttctggat actgttacct
     7981 aatcccctgc caaatagtcc tccgttgaac atggcatagt agccgttgac tagttgatgc
     8041 cctttattta attcgtctga gaaaggattt tggtaaatag caaatcgatt atagatatac
     8101 tgtaaacgtc cgggtaagat ctttccgttt gtgatattga tcagccagat cgtaaaagta
     8161 cttagacaaa agcctcctac tcctacaatc aaagtataca cataattgac tccgctggct
     8221 aaaagcagaa ccaaaataat caaaaagata actgccgcat taccaaaatc gggaagaata
     8281 gcgactaatg cagtaagtcc gataaccatt gcaagtggac gtttcacagt cccaagaaaa
     8341 tctttttgta cactgttttg tcttttagat aaagtcagag acaggtacca gatagaaatg
     8401 atttttaggt attcggcagg ttggatcgag aatcctgcaa tttgcagcca acctttcgcc
     8461 ccattgatct ccttaccaaa aaacacaaca atcagcagca aaatcgtcaa aactgcgatg
     8521 gctgccatga tcaaacgctg gtttttcaaa acatctgttt tcattttata aagtagtgcg
     8581 atagcaatca agctaagcac ccaaaaaata ctctggttaa tgactgatgc ggacggattt
     8641 tgtccgtttt ctaataatag atacgacgta gaactatata ccatgatcaa tcccaataca
     8701 cataaaatta aatagggaat caagatgcta tagtctaata gatgcctttt tttcactttc
     8761 ttaggcaagt ctattcctcc ttgccaactt cttttttatc ataaatttct tggtaaacct
     8821 cactatataa ctgattcaat tctcgttcta gctcacttaa catcagctgg ccctcgttct
     8881 ttccgattac tcctatacgg acagcaaaac cgacttgccg ggaaaagccg tacatctgtg
     8941 tatctaccac ttcttcgaag gctttgcatt gtgagataca gagactgttg ttttgatttc
     9001 ggattaatcg tttgatccgt tctgcttcat catttaatac ttggatcgca agttttttcg
     9061 acaaagaatc catctgtgct tacctcccat tctaattcct taattataac atatttgatt
     9121 ttcataagga gcaaaaaaaa cagatttaac cagttgtttt tattcatttt tacgtattcg
     9181 ttctttctac ttatccagaa cgttagagtc cgcatttttt atccgagata aaaaggcttc
     9241 gccatattta tcaagcttgc tttggccaac tccttttatc tgaagcatct ccaatctgct
     9301 tgatggacgt attcggctca tttctttcaa tgtggaatct gaaaatacga catacggggg
     9361 aacgcctgct tctgatgcta gatccgttcg cagttcccgc agcacttcaa atagtgtatc
     9421 ggtttcttca tccgatagct gctggacttt cttgggttcc tttcgataaa cagattcttg
     9481 tcctttcaat acttgtatcc ctctttctgt tactttcaaa acaggatact caccattgat
     9541 ggtcagaaga tagccattcg atatcaaata ttcaattaac tgcatcgttt ctttttgcga
     9601 ttggtttttc atcagtccat aggtagataa atgtccaaaa cctaaagctt gcagcttttg
     9661 ttctttcgat cctgctaaga ctttcatcag catttgttta ccatagttct cacccatacg
     9721 ttttaagcaa gaaagaacca tctgtgcttc tgtcgttact tctactattt ctctgtcatc
     9781 taaacagtta ccacaacggt tgcacggact gcttgtctcg ccaaaatagt ttaggatgta
     9841 ttgctgaaga caggactcaa tatacacgta ttctgtcatc gcttttaatt tttcatattc
     9901 tttttgcttt tgcccttctt ctctttggga ctgctggata aaaaactttt ggacttggat
     9961 atcttgagga gagaaaagca ggatggcttc actggaaaga ccatctcttc ccgcccgacc
    10021 agcttcctga tagtatgctt ctagagagcc aggaatttga taatggataa caaaacggac
    10081 attgctttta tttataccca taccaaatgc atttgtcgct acaatcagct gcaagcgatc
    10141 gtataaaaag gcttcttgca tttctgttcg ctcattctca tttagaccac catgatatct
    10201 acccacggaa aaatcgaatt ttttaagcaa atgatagaga cggtctactt cttttctcgt
    10261 tgctgcataa atgataccag attgattttt gttgatcttt aaatattcga ttagatattg
    10321 ttcttttttt tgatctttaa ttacttgaaa tcgtagattt tctcgttcaa aaccagtctg
    10381 aatatggttg ttttctggaa tttttaaaag tctttttatg tcagcagcta cttgcacagt
    10441 cgctgtagct gtcaaagcaa caattacagg acgatttggc agttgatcta gctggttcgc
    10501 catctgtaaa taacttgggc ggaaatcatg cccccactga gagatacaat gtgcctcgtc
    10561 aatagcgatc atacggacgt tccatcgctt cattgcttgg ataaaatccg gcatgataaa
    10621 acgttcagga gcgatataaa gtaaacggta ctgctgtcta tcgatttctt ggaagcgtct
    10681 agctgtttcg taaccatcta acgagctatt gataaaagtg gcgggtatac ctaattgatt
    10741 cgccgcgtcc acctgatcct tcatcaaaga gattaaaggg gacacaacca cagtaagccc
    10801 atctaatagt aaagctggaa gctggtaaca gattgatttc ccacttccgg taggcatgat
    10861 tcctaataca ttttcctgat caatgacttt ctggataatt tctttttgac caggtctaaa
    10921 ctcatcatag ccaaagtatt gttttaataa gtcgttcatc ttcccgctcc tttctcttgc
    10981 cattttactt cgattttctt cgaaggacaa gttgatctcc tttcgtgaaa cattatctta
    11041 ttatgaaaca aaaactctct agaaataagt tttttaaaag tcttttaaac gtctttcggc
    11101 gtattttctg aatttatgtc cattgacaaa gatgacttct ccacagaaaa gagttggaca
    11161 aaaaaagaaa ccagctatt
//
LOCUS       DAHEYX010000087        11077 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHEYX010000087 DAHEYX010000000
VERSION     DAHEYX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.088"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            166..1122
                     /locus_tag="MB219_001545"
     CDS             166..1122
                     /locus_tag="MB219_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCC8427264.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1212..2315
                     /locus_tag="MB219_001546"
     CDS             1212..2315
                     /locus_tag="MB219_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCC8427265.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2328..3281
                     /gene="menA"
                     /locus_tag="MB219_001547"
     CDS             2328..3281
                     /gene="menA"
                     /locus_tag="MB219_001547"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCC8427266.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4095..5441)
                     /gene="gor"
                     /locus_tag="MB219_001548"
     CDS             complement(4095..5441)
                     /gene="gor"
                     /locus_tag="MB219_001548"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCC8427267.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5575..6828)
                     /locus_tag="MB219_001549"
     CDS             complement(5575..6828)
                     /locus_tag="MB219_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCC8427268.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6834..7757)
                     /locus_tag="MB219_001550"
     CDS             complement(6834..7757)
                     /locus_tag="MB219_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCC8427269.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7978..10470)
                     /locus_tag="MB219_001551"
     CDS             complement(7978..10470)
                     /locus_tag="MB219_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCC8427270.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10477..10944)
                     /locus_tag="MB219_001552"
     CDS             complement(10477..10944)
                     /locus_tag="MB219_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCC8427271.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3265 a         2261 c         1941 g         3610 t
ORIGIN      
        1 tccttttgga tttttttcaa attttaaaat aaagcgtttt ttcacgaaat ttgctgaaag
       61 aaaacgtttg aaatattaca tgggttattt atcaaaaata gttgacttat aggctaaaaa
      121 gattagaatg tataatgaat cacaaagaaa aattgtggac tagaaatgga atggtggggt
      181 attaaaatga agaaaaaacg ttttgtaact ggatttgccg tattagcatt ttcagctcta
      241 gtgttaggcg cttgcggcgc agatgacaac ggaagctcaa actcttcttc tgaatcatct
      301 acagcacaat cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt tgtagctggc
      361 ggagatttgc aagacggcac atataaatta gaagaaaaaa attattcaaa tggttaccgt
      421 gcagtctttg aaatggttgt aaaagacggc aaaatcactg aatcaaaata tgataacgtc
      481 aatgaaaacg gcgaatcaaa aacaaaagat gcagaataca acaaaaacat ggaagcaaaa
      541 tctggcacaa acccagaaaa atacattcct gagttgaatg accaattctt aaaagcacaa
      601 agcgcaagcg gtgtggaagt cgtaactgga gctactcact catctgaaag cttccaaaac
      661 tacgcacaac aattgatcca agctgctcaa gctggtaaca cagatacaat cgaaatcgac
      721 aatggtgctg atttgaaaga cggcacttac aagcttgaag aaaagaacta ctctaacggc
      781 taccgtgtgc aattcgaaat gacagttgct ggcggcaaag taactgaatc aaactttgat
      841 tatatcgaca aagacggtaa atcaaaacaa gatgatacag aatataatga aaatatgaaa
      901 gcaaaaagcg gtacagaacc aaaaacttac atccctactc tgaacgacga attcgtcaaa
      961 gcaatgggcg aagaagatgg ctcacctgct aatgtagaag ttgttacagg tgctacacac
     1021 agctcacatt cattcatcat gtacgcacaa caattagtga atgcagctga aaaaggcgac
     1081 actcaaacaa tcgaagtaga taatatcgta acagaaaaat aatcacataa tcatactaat
     1141 aaaagttgga gggtgggacg atagccagat taggcagtcg tctcaccttt caaaatgaag
     1201 agaggaaaaa gatgcggaaa aaactactat tttccttaat cacactgggc atctctttta
     1261 tggtgctaac tggctgcagc aactcagcaa ataaaacaac cactgaaagt tcagcagcta
     1321 ccaagatatt gaaagatcca tattctgatg aacagttctt gttagggact tacgttcgta
     1381 tccgtgtgta tgatgaaggc aaagaatcag cattgaaacc tgccttcgat cgagtaaaag
     1441 aacttggtga taaaatcacg atcaatcaaa aaggttctga gatcgatgaa gtgaatgagc
     1501 aagctgggat caaaccagta aaagtttctg atgacgtcta cacgttagtc aagcgggcct
     1561 acgaatatag ccaagattct caaggcggat tcgatatggc gattggtgca atcacacaat
     1621 tatggcgaat cggttttgat gacgccagaa aaccttccca agaagaaatc gatcaggcat
     1681 tgaaattagt ggattatcat aagatcgaac taaatgacaa agaaaaaact gtttatctaa
     1741 aagaaaaagg aatgatcatc gatttaggtg caatcgccaa aggttacatt acggacgaag
     1801 tagtgaaagt cttgaaaaaa caaggagtga ccactgccat cgttgacttg ggcggtaatg
     1861 tctatgtctt aggccacagc ccaagaggcg aaaaccaaga ctggacagtc ggcatccaag
     1921 atccgaacat ggctcgcgga tctgttttag gttctattaa agaacggaac aaaacattag
     1981 ttacctctgg aatttacgag cgttatttag aagtagatgg caaaaaatac catcatttgt
     2041 ttgatccaaa aacgggttat ccgttcgaca atgatatcgc aagtgtcacg atcataacag
     2101 ataaatcaat tgatggtgac gggttgtcta cagcagtctt ttcaatgggt gtgaaaaaag
     2161 gactggaata cgtcgaaagc gaattgaata acgggacgga agcgatcttc gtgacaaaag
     2221 acgataaagt atatgtgacc gatccaatca aagacacctt caaattatcg gaggactccc
     2281 attacacgat gggtgaccgt tctgaactaa aataaggagt acgaaccatg acactaaaaa
     2341 tctttttaga agtagttgaa atacgaacaa aggtggctag tgtatttcct tttgcggtag
     2401 gggtattatt ctctatcgct ttttttcatg agatacattg gctaaacact tttttatttt
     2461 ttattggaat gctagttttt gatttagcga ctaccgctat caataattac atggattatg
     2521 aaaaggctca ttctgaagta tataagtatg aagaaaatgt cattggccgc agaaatctat
     2581 ctcctgtatt gattcgaaac atgattttag ctatgattgc atttgttttt gttatcggcg
     2641 ttattctaac gatcaatacg ggttggcttt tacttttgat gggatttatc tgttgtttta
     2701 ttggagtatt ctatacattc ggaccgattc ctttatctcg aatgccttta ggggaaattt
     2761 tcagtggggt gactatgggc cttggaattt ttgccatggt cgtttatgtc aatacattcg
     2821 gttcagatgt ctatgatctg acagtcaact tttcatctgg acaatttgat ttggtaggaa
     2881 atctttgggc tatagcagca atcgtattag cttccttacc cttggtgttt acgattgcca
     2941 atatcatgtt ggctaataat ttgcgggatc ttgaccgtga tattgaaaat catcgctata
     3001 cactagtttt ttatatcgga cgtcctgcgg gaacagtctt attccagcta ttgatgtatg
     3061 cttgctattt cgtgatttta atggggttgt tgagcggtat ctatgaatgg ccgatcttgg
     3121 tcaccttctt gaccttgcca attgtgaaga agcatttgga tcaattcaag caaacgttac
     3181 cccagcctat cagtttcagc tattccatca aaaatatggt cttattcaat gccagttatg
     3241 tattggggct attactaaca atcattatgc aagccattta acagcatgaa gaggttgtga
     3301 aaaaactgcc ctgcatcaag taataagaac agccaaataa aaatagctcc ttatattttt
     3361 cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttat caggaggttg
     3421 tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
     3481 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt atcagaggtt
     3541 gtaaaatcag tgttttgtcc tgagtattag agaaacaaat ggaaaaatag ttcctcatat
     3601 tttgccgttt tttgagctaa taccgaagga cagctgattg aacactattt gttcggaaaa
     3661 agtgactaac tatgacacaa tttttttcac agtccctttt ttcacttgat ttgattcgta
     3721 ccagcgaact tgacgagaac tttcttggtt tggtaactat atggtattct tttaacatcc
     3781 tcgtttgctt cttctaattt atttatacaa agtcacactt ttttaaacat ctgttcttcg
     3841 atacatttct atttctttta aacatcgata ccaatatttt gaacttaaga gtgcatacca
     3901 aatggattca tttactcttc gtcagttacg aagataatca aaatctccaa cttccctact
     3961 tatttcgttt tgagaattat cgaagcaatc ttacataaat aactaaaaaa caaaaaacca
     4021 gaaaatcagt atactgattt tctggtttta catggtttgt tcaataaaaa gctgttggaa
     4081 ctgttaatat taaattatcg catcgttacg aattcttccg ctcctgttgg atggatggct
     4141 actgtattgt caaagtcttc tttagttgca cccatcttga tagccacggc aaaaccttgc
     4201 agcatctcat cgacaccaac accaatagca tgtaagccga cgattctttc ttcttttccg
     4261 acacagatca atttcatttc gcatttttgg cgataatcat ttaatgcaaa gtacattggg
     4321 gtaaagcgtg aacgataaac tttcaattca ttttcgccat atttttccaa tgcttcttct
     4381 tctgtcaaac caattgttgc aattggtgga tgcgtaaaga cgactgtcgg aacaagttta
     4441 tagtcaagat aagaatcttt ctttccatta aataaacgtt ctgataaacg tctaccagct
     4501 gcgattgcaa caggagtcag atctaattta ccgatcacat caccaacagc aaaaatatgt
     4561 tcatccgttg tattttggta tttatctact ttgataaacc cttttttgtc caattccaca
     4621 tttgtatttt ccagacctaa tttttcggta ttcggtacac gtccaccggc aaaaagtaca
     4681 caatcaccag ttaaggtttc accattttca aatagcaccg tatattcgtc attgttttta
     4741 gtaatttctt tagcaatatg atgtgcgtaa gtttttattc cagcttcttg gtacatttgg
     4801 atcaaattgt ccgaaagcat tttatcaaac gttctaagtg gtcgttcttt tcgatatgcc
     4861 cacatgactt cactacctaa accacgaaat acacctgaaa gttcagccgc aatgtatcca
     4921 gcacctaaaa cgattgctct cttaggtaac tctgttaatt caaaaaatcc atttgaatca
     4981 attgcgtatt ctccaccagg aatggtcatt ttacttgcac ggcctccagt agcgatcaag
     5041 attttaggag cacgataggt ttcaccgttt acttcgatcg tttgactatc gataaaagta
     5101 gcgtaacctt ttatagcctc gacatgattg ctatctaatc ctctttgata agcaccgtgt
     5161 agaaaatcaa tatatttttc tcgtctttct actagaccgg cgaaatcaac attttttaaa
     5221 tctgcttgga taccgtaact tgaggcatct cgttcaattg tttccaaaat cgtacttgct
     5281 tgccacataa cttttttagg tacacaaccg acgttgacac atgttccgcc caattcattc
     5341 gcttcaatca gcaaaacttt tgcgccatgc atccctgctc tgtttgctga agcaataccg
     5401 ccagagccgc caccaattac gatgtaatca tatatttgca ttttgttcct ccttattgaa
     5461 gagatcaatc aaaaccttta tttaacgatc ctttttctac agaaaacaag tgtaacactt
     5521 ttcttgaatt ttgtatgcga agatgcttaa aacagggata tatcagcttt ttccttattt
     5581 acgttttcgg atcatatctc caggtttcac cggctgtgcg actggcatag ttaaaatcat
     5641 cattggattt ggtgctcggt caatggattc gccttcttca ttatgcataa cttcaacggt
     5701 ttgactgaaa tgactgaatc ctggaccata aaattcgatt tcatctccta cagaaaacaa
     5761 gtttctctga cgtatcgtag caatctgtgt tgcctcatca taagataata cttcaccgac
     5821 aaatttatat actgggattt tacgacgttc accgaataat tgttcgtttt cagttggtgt
     5881 attgtagtaa aaacctgtag ctaattctcg ttgtgctact ttccataact cgtcgatcca
     5941 ttcttgcttg cagacatagt tttcaggatc ttccatataa ctgtctacag ccgctttata
     6001 gacatttgac accgtagata cataatggat cgatttcatt cttccttcga tcttgaagct
     6061 gtctacgccg ttttcaatca attctggaat atgttcgatc attgacatat ccactgcact
     6121 catcgagaat tcttcttcca cttcgcctgt atcagttaaa ctgtttcgtt cttgaccaaa
     6181 aggcatatcg aacaatccat acttccagcg acaagattga gaacatccgc cacggtttgc
     6241 atcccgcatc gacatatggt tggacaatgt gcatcgacca gaataagaga tacacatggc
     6301 accatggata aatgcttcga tttcggtatc cgtattttta cgaatctctg ccacttcctc
     6361 cattgaaact tcacgagcta aaacgacacg ttccaaccct tcttctttcc agaattccaa
     6421 tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg atcggcagac ctggggcttc
     6481 tgtcgcacaa atttcgatca aagctggatc agaaacaatc actgcagaaa taccgacatc
     6541 acgcaaactg cggaaaaatt cccctgctcc atcttcattt ccttcatggg tgaccatatt
     6601 agcagcaacg tataccttcg catcatgttc ttttgcaaaa gcaactcctt cagccatttc
     6661 ttcataagta aaattacctg cacggcttct taaaccatat gcattcccgc caatatatac
     6721 tgcatctgct ccataatgga tcgcggtttt taacttttcc agtgttcctg ctggcgctag
     6781 gatttctggt cgtttcaatt ttctttttac tgtcatttct ttcatcatac tccttatttg
     6841 atagcagaag gatcaatata aaagaatcct gtatctaatt cacggttttc aggatgcaat
     6901 ttactgattt cgctagtcat tttttccgct ttttcttgat cccagctacc ttctaaaatt
     6961 tgtttttttg cttcggcgaa tagtcgagtg atgtttacga atttttcgcc tggagtaaat
     7021 aatccgtcca atttccatgt acagtaatga tgctgataaa gttcattcaa ttcattcgag
     7081 agattcagat cattgctggc aaaaatatgt gtgccatgac tatcttcata aatagaataa
     7141 tgtgtctctt cttttttagg ttccgagata aaaagacctc tttcacgatc tttttgttcg
     7201 tcttgtttcg tatagttata gtaattctgt agcagtggac gtttggattg atggatgcat
     7261 gtagcaccat acacaagggt ttctacagga atatcgagtt tttcttccat cgccttcatc
     7321 tcttcaaaag gaacttcacg agcaagtact gctccaactg cgcctttttt cgcccagaaa
     7381 ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat aaatatacgg tatacgcaat
     7441 tcaggatttt ttttcatcgt ataaataata cctgtatcac caactgtgat cacatctacg
     7501 tttatttctt ttaaaaaagc caaatattca ggtacatttt tcattttttc aggatgaatg
     7561 atcccattta cagcaatatt cgctttttta ccatacttgt gtgctaattc taccaattgt
     7621 cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc caaattgctc ttcaccgaaa
     7681 tatagtgtat ctgcacctgc agcaagcaac tgttctgctt gttcaatagt ttctactgtg
     7741 gttatgagtt cgatcataat tttctcctcc tacaacttca taatagtagc atagctctaa
     7801 aaactttcca atacttcttc tatttaaagt cggatttttg aaaattattt cacaaaaaat
     7861 caaaataaag ttcgatatat tctgtccaaa tataaaaaga gattgtgaca aaaatcttgt
     7921 cacaacctct taatcaagtc ttactattat aaaaattcag ctacacaggc caagtaacta
     7981 tagggaacct actgtttcct ttttaggtcc gcgaacattc aatgtgatct taccttttga
     8041 agcacctact gttacgtggt cacctaaacg aatttctccg ccaagcaaag cctcgctcaa
     8101 tcgatcttca atttcttttt gaagtgcgcg acggattgga cgtgcaccat attcaggatc
     8161 aaatccagct ttcccgatca catcgattgc agatggtgtg attttcactt gaatatcttg
     8221 ttctttcaat cgtttgataa tcgctttgct catgatttgg acgattgtat ggatttcatc
     8281 tttactcaat gaatggaaga cgacagtctc atcgattcga ttcaagaatt caggtcggaa
     8341 agctttcttt aactcttcta agatacgttt ttgcatggct ttgtgatctt tagtaacatc
     8401 ttggacattg aatccgacat ttttttcttc tcgaatttgt gtcgctccga tatttgaagt
     8461 cataatcatg attgtatttc taaagtccac ttttctacct ttagaatctg ttaaatgacc
     8521 atcatccaat acttgcagaa gtaaattgaa aacgtctgga tgtgctttct ccacttcatc
     8581 taagagaatc actgaatatg gtttttgacg aattttttct gtcaactgtc caccttcatc
     8641 ataccctacg tatcctggag gagacccgat taatcggctt gtactgtatt tttccataaa
     8701 ttctgacata tcaacacgga tcagggcatc ttcacttcca aacatgactt ctgaaagtgc
     8761 tttggccaat tctgtttttc cgacaccagt tggtccaagg aacatgaatg aaccaattgg
     8821 acgatcagga tctttcaatc cgcttcgtgc acggcggatc gcacgtgaaa ctgctttgac
     8881 agcctcatct tgaccaacga cacgttcatg cagaagtcct tccagttcta acaagcgttc
     8941 gctttctttt ttctccaact gctgtagagg gacaccagtc cactccgata caactgtagc
     9001 aacatcttct tctgttacac gatcagaata accttgctcc tgttttgctt caattgcaga
     9061 aactttttgc agctttttac gtagtgcttt ttcttgtctg cgtaattgag cagcattttc
     9121 aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact agttcatcga tttctagttt
     9181 gatcacagtt gattttgtta agtgatcggt ttgatccaaa cggactttcg ctgctgattc
     9241 atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa gtgatataac gaacagaaag
     9301 attgactgct gctcgtacag cttcatctgt gatctccact ccatggtgct cttcatatct
     9361 tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga gttggctcat ctacttgaat
     9421 gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata tatttttgat attcatctag
     9481 ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac gccggtttca agatattcga
     9541 agcatcaatt gctccttctg ctccacctgc accaattaag gtatgcaatt catcaatgaa
     9601 taagattact tgaccatcat tatagatttc gtcgatgacc tttttcattc gatcttcaaa
     9661 ttcaccgcga tatttcgtac cagcaaccaa agatcccata tcaagcatca tcaggcgctt
     9721 ttcttgcatg tcttctggca cttcacctaa aatgattttt tgagctaacc cttcagcaat
     9781 ggctgttttg cctacaccag gttcacccac cagtacaggg ttattctttg tccgtcggct
     9841 aagaatctgg ataagccgtt tcacttctct cgaacgacca acaactgggt ccagcttgtt
     9901 ttcttttgct aatttcgtca aatcacgtgc taatgaatct agcgtaggtg ttccttgcgg
     9961 tgtttgccgt ttatcttgta catttacatt tctacgacga ttcattcctt ttccagcaga
    10021 atcaccagct agccccattt tttgtttcag aacttgacgg gcttttgaaa ggctgatacc
    10081 taagttgatc atgattcgag tagccatgat actttcatct tttaataagc tcatcaaaat
    10141 atgttctgtt ccaacttttt gagaaccagt tttcttcact tcgctgctag ctaaagcaaa
    10201 aacttgtttc attcttggtg aatatggaag aaagactcct ttagggtaag aacggatacc
    10261 accataacca atcagatgtt caatttcttc ataaatttcc gtttcattca aatccatttc
    10321 acgtaatact tttccagcaa ttccattttg ctcgaccact aaggctagta aaacatgttc
    10381 tgaactaact gattgatgtt tcaaccgttt tgcttcttct tgtgcaattt ctaatactgc
    10441 ttgggctctt tcagtaaaaa gttcttccat ctaatctcac ttcccatctt cataacttaa
    10501 acgttccaga aatgctcgga gaatccgagc acgtgtatga tcttcatatt cattgaaatt
    10561 caatgtgttt tttgcaattg cactcaacat caagtttcct tctttttttg taattatttg
    10621 gtcttcataa agtttttgaa taatagcaaa agcatttttc tcactgattg tatcatcaaa
    10681 taactgattt atctgctcaa gtaattgttt tttatctgaa atccttactt ttgcaattcg
    10741 tatataacca ccaccaccac gtttgctttc tactagataa ccacgttgga tggtaaaacg
    10801 ggtattaatc acatagttga tttgtgaagg aacacagtta aagaggtctg ccatttcaat
    10861 ccttcgaatc tcgatcattt cactattttc cagaattttc tttatatatg attcaattaa
    10921 atccgaggta ttctgatgtg ccatcttatt cattcccttt gactaatatt gaccttaatt
    10981 ataacgaatt tgataataga aagaaagaag aacgcttttt gttaaataaa tcctttagaa
    11041 tcaacaaaaa aaaatacgga atatactatt ccgtatc
//
LOCUS       DAHEYX010000088        11055 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHEYX010000088 DAHEYX010000000
VERSION     DAHEYX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.089"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..598)
                     /locus_tag="MB219_001553"
     CDS             complement(<1..598)
                     /locus_tag="MB219_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427272.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIE"
     gene            complement(611..2113)
                     /locus_tag="MB219_001554"
     CDS             complement(611..2113)
                     /locus_tag="MB219_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427273.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2661..4340
                     /locus_tag="MB219_001555"
     CDS             2661..4340
                     /locus_tag="MB219_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCC8427274.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4467..4946
                     /locus_tag="MB219_001556"
     CDS             4467..4946
                     /locus_tag="MB219_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCC8427275.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5165..5845
                     /locus_tag="MB219_001557"
     CDS             5165..5845
                     /locus_tag="MB219_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCC8427276.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5853..7184
                     /locus_tag="MB219_001558"
     CDS             5853..7184
                     /locus_tag="MB219_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCC8427277.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7199..8161
                     /locus_tag="MB219_001559"
     CDS             7199..8161
                     /locus_tag="MB219_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCC8427278.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8272..8610
                     /locus_tag="MB219_001560"
     CDS             8272..8610
                     /locus_tag="MB219_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCC8427279.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8689..9396
                     /locus_tag="MB219_001561"
     CDS             8689..9396
                     /locus_tag="MB219_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCC8427280.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9499..10980)
                     /locus_tag="MB219_001562"
     CDS             complement(9499..10980)
                     /locus_tag="MB219_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427281.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
BASE COUNT         3514 a         1925 c         2324 g         3292 t
ORIGIN      
        1 cttcaatctc atgagtcacg atgataatac agcgattttg ttcatgggcg atttcttgga
       61 aaagtttgac gatatcttgt gaagttgttt catctagatt tccagtaggc tcatccgcta
      121 cgattagttg atgttcacag cagatagcac gaacgatagc gactcgctgc tgctgacctc
      181 ccgaaagttt ggtcacctgt ttttttgcta atatttcatc aattcctaca cgagccagtc
      241 gatctaaagc aacttgtcga taattctctt gcttggtctt agtgatttcc attgctgcta
      301 tgacgttgtc tagagccgat aaataaggaa gcaagttgta agcttggaaa ataatcgaga
      361 catcattttt acgataactt cttaacccga ttttgttcag tgaacgatct tgatagtata
      421 cctttccttc ttttggtgca tctagtccag aaataagcga aagaaaagtc gtttttccag
      481 aaccacttgc tcctaaaata gcgtacattt ttccagcttc gaaattcagt tctacatctt
      541 tataaagcgc atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc cgtaacatca
      601 aattcctcct ttagtttaat aggatttttc ttgggtttaa tcttaatatc cctatagaag
      661 ctaaaaagac agaaaacaga ctgattccaa atgctactcc ggctaatagt cccaattgtg
      721 caggtttgac attgatttcc aattcgctga cttgatcaga aacagcaaat ggattattcg
      781 tggttccttg ttgaccatcg cgttgaccgc cttgttcttg tctgttacct ggcatttgtc
      841 caccgccagg agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca agtagctgct
      901 gtcccacggc attcgcgacg acgtttccgc taagcgaagc aactcctaat gcaagaatca
      961 tacaaatagc cacttcggta aacaattgga gaatgatttt acttctggat tctcctaaag
     1021 atagcaagac gcctaactca tgccgacgct cccgaatggt aatcataatg atcagagtaa
     1081 gtatgataat accggctaca gccactagta aaacaacatt tttagaaaaa cttgccacgt
     1141 tgtttaaagg ttccaacatt gactgataca tggaatcatt tgattgtaag ctaaatgtat
     1201 ctgtgtcgat cagcttctca gcttctttga gaaactcatc cattttatct ggatcagata
     1261 gagtgtaaat agcagaatct atcgtgttat cgtcagatgt cccatttagt tcatttgcaa
     1321 aagtgtaaga agtgtaaagt gtattcgacg gattcatgaa gttgaatttc atacccatac
     1381 tgccgcttgt ttcgctggat tcataaattc ctttgatcgt catttcataa gtggtatctt
     1441 catcttcagt actcgtgatt gtgaaagtat ctccgactgc caggtcattt gtttctgcta
     1501 gagtagaatc aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt ccttcaccat
     1561 ccgtgatttt tgctgttcca tcagaaaaat tactattttg tgcagttgct gacacaccac
     1621 tgatttgaaa gtctgcttga gtcatttgcg gattcccgcc cattcctcca gcaggttcac
     1681 cgcctgcatc agtagtttct gaactgtctt cacttgaatc agaactagtg atggcagtta
     1741 ttccatcttt tgccatagct gaggcggaac tttcaaagga ataactcttt acattatcta
     1801 attcagctat tttttccgcg tccgttaagg aaactggtgt aagagaaaaa ctgcctggat
     1861 ctggacgttc ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt ttaaatgaag
     1921 cttctcgatt ggcacttaaa gtcactgttg ctccaacttc tttttgtgct tgattcgctg
     1981 cttcttctgc agcgcttcga atcgtgaggc cagctagcac aaaaataaga atggctgtga
     2041 acacggcaaa caataacaat gaacgtcccc attttacttt tgtacttttc catgcacgtt
     2101 tcataaaatt catattttgc atccctccta gtagatgcat ttgataatag acaacctatc
     2161 ttaaagctag cttaaatctt atgctgtttg cacaccatca acatgaggtt ctgtatatat
     2221 aaaaagtcac tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat gtttcaaacc
     2281 gacgaagaag aaatgaaaga ggaatagcaa aagaaatcct agatgctaca gagggatttt
     2341 tacgtgcata gttgttctaa caattgctga tactagcgat ttagtttatt tgttgtataa
     2401 taatggggaa cagctgaata cttatataag tattctggct atagcgtttc gcaaaagatc
     2461 cattttgtta atgaagtata tccaaaataa aatgcccatc caactgtcag aacgcggatg
     2521 tgctggtaat tttcctgctg attctccata agttgagaaa tagtgatttt gcatatgctt
     2581 cattggaatg gctcatatat ttagataaag aagaaaatcg acatctttaa acttggatgt
     2641 cataagaaga ggtgaagttt gtgagtacca ttaaaattat cccattaggc ggcgttcgtg
     2701 aaaatggaaa aaacatgtat gtcgcagaag tgggcgagga tatttttatt ttagattgtg
     2761 ggctaaaata tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct gattttacgt
     2821 atttagaaga aaacattgat cgtgtagctg gtgtcttttt aacacatgga catgcagatg
     2881 cgatcggggc acttccttac ttgttgtcaa aaatttctgt accagtattc gggacagagt
     2941 tgacgatcga gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa ggatttaagg
     3001 atttccatgt ggtagatgag catacagaaa tagactttgg tgaaacagta gtgagctttt
     3061 tccgtaccac tcatacgata ccagattcag tggggatcaa tctgaaaaca aaagaaggta
     3121 gtatcgttta tacgggagat tttaaatttg accaaacggc tatcccaatg tatcaaacag
     3181 attatgcacg attggcggaa atcggcaaag aaggagtctt agccttacta agtgaatcgg
     3241 caaatgcaga aaatcctcag cctgtagctt ctgaagccca aatagcagat gaagtatttg
     3301 atacgatcaa atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt aatttgcaac
     3361 gagtgcaaca agtattggat gcagcagaaa aagcacaacg aaaagttgtc ttgactggtc
     3421 aagatttcga acgaattatt cgcacagcga tgcgtttaga aaaacttcaa ttaccaagtg
     3481 aagatctgct tgttacgttg aaagacatga aaaactatga tccagaagaa ctgatcattt
     3541 tagaaactgg ccgaatgggt gaacccatca agtctctgca aaaaatggca aacggtacac
     3601 accgtaatgt caagattgaa gaaggagatt tggtttatat cactactacg ccaagcatcg
     3661 cgatggaaac catcgtggcg aaaacggaag atatcattta tcgagctggt ggaacagtga
     3721 agttgatctc tgagaatatg cgcgtttctg gccatgccaa tcctaatgac ttgcaattaa
     3781 tgatcaattt gatgaaacca acatattttg tgccagttca aggagagtat cgtgaattag
     3841 ctgctcatgc agatttggct catgcagtag ggatgccata caaaaatatc tatattactg
     3901 gacgcggaga cgtgctggaa tataagaaca agcgtatgag tgttgcagga actacaagtg
     3961 cagaaaatgt aatgattgat ggattaggca taggtgatat cggaaacatt gttttacgtg
     4021 atcgaaaaat cttgtcagaa gacgggattt tcgtagcggt tgtcactatc agtcggcgag
     4081 aaaagaaaat catttcaaaa cctcaaatta cttcacgggg attcgtttac gtaaaagcta
     4141 gccgtgatct gatgaaagaa agcagcaata tgattgaaac aatcgttgag aaacatttag
     4201 aaagcaatga ttttgagtgg agcaaattaa agcaagacat ccgagatcaa ttaggccgtt
     4261 acttatttga acaaacaaaa cgccgcccag tgatcttgcc agtcattatg gaagctactc
     4321 aacgtcgaaa taaaaaataa acaaaaagag taagatgaac ctctctatag agtgctcgtc
     4381 ttgctctttt tttgcgaaaa acaaataatt tttgaataca gcatccctcg ctttataata
     4441 aacgatagaa taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa cgttatttag
     4501 aagaacaaaa gatcagctat caaccacttg attttagtca tgtggtagaa gaatctttca
     4561 cagaagaact acaaaaaaga gggattgatc cagctttgat atgtaaaact ctcgtagtga
     4621 aaggaaacaa aactggggtg atcatcgcat tgattccttt gaacgatcac ttagattaca
     4681 aaaaaacgag acaagtgacg aaagatcgta aagttggttt tcctggaatg gattttgtat
     4741 taatgcatac aggatatccc cacggagcaa acacccctat tggaataaaa ctcgcacatc
     4801 ctgattattg ttttttagca gacagttctt taaaagaaaa agaagaagta attgtgtcaa
     4861 gcggtgaaat cgggagaagc cttcggataa aagtagccga tttagaaaag atcgtccatc
     4921 ctatttacgc tgatttaatt agttaaggga cgagaagaga agctaagcag agcagagacc
     4981 tcaaacttga gattatatct gcttggtact tggtgcatcc tgtattatct gttacaattg
     5041 ctttgtacat actgtactat caatccaatg aaaatatatt tagcagctat attttttcat
     5101 tgatgtgcaa gtgatgaaat cgtttttaat aaaaaagata cagagaaaac gcgaggtgta
     5161 aaatatgaaa attacgtatc atggacattc ctgtttaaca attgaaatgg aaaatggcca
     5221 acgcgtgatg attgacccgt ttattacagg aaatccattg acagacttaa ctgcagaaga
     5281 agtaaagaca gattggttgc tgattactca tggtcatagt gaccacatag gagatatgat
     5341 tcctattgca aaagaaaatc aagccacaat tatcagtatt gttgagattg ctgattatgc
     5401 gaacagcaga ggagtagatt cattcggtat gaatatcggt ggaaaacatg cgtttccatt
     5461 cggcgtggta aaattcgtac atgcacagca tagttcaagc tatgaagtag acggtatcgt
     5521 tcagtatatg ggcgagccta gcggaattat tattcaagca gaaggaaaaa cgatttatca
     5581 tgcaggggat actgcttatt tcagtgatct aggattgctt gcagaagaat tcgatattga
     5641 tgtcgctttc ttgccgattg gcgataacta tacgatgggg ccagaagatg cagcaaaggc
     5701 tgcgcaaaca attcgtgcca agaaagtagt tccaatccat tacaatactt tccctgtcat
     5761 tgaacaagat cccgaatctt ttgtagagtt actggaagat agtgaaggaa aaataatgag
     5821 cgtctcggaa acgctggaag tgtaggcaga aaatggcgac aaaacatgat ttgattttag
     5881 aatatattga aagtttacct gttggcaatc gaatttctgt aagaagcatc gcaaaagaat
     5941 tgaatgtcag tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat gtcggactag
     6001 tttcaacaat tcaacgagta gggaccatcc gtatcgaacg caaattaaaa aaacatattg
     6061 aacgtttgac atttggcgag gtcgtcagga tcattgaagg ggacgtttta ggcggggcgg
     6121 caggattaga caaggtcttg aataaattcg tcatcggcgc gatgactaaa catgccatgg
     6181 aacgctatat tacgccagga tcactaatga tcgtcggcaa ccgggcggaa gtccaaaaat
     6241 tagcattgga agatggcgcc gcagtattga tcacaggagg attcaatacg actgctgaga
     6301 ttgcaaagct agctgatgat cttgaaatgc cgatcttacg cacctcttat gatacattta
     6361 cagtcgcaac catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa gatatcatgc
     6421 ttgtcagtga tatctatatg ccagctgaaa agactcatta tttgcagcag acagatacga
     6481 tttctgatta tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca gtcgtcaacc
     6541 gccatcatcg tttgatgggg attgttacgg caaaagatgt tttagggaaa tcgccaaacc
     6601 agatcatcga tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca atgagtattg
     6661 cctctgttag ccatcaaatg atctgggatg ggctagaagt catgccggtc gtttccgatg
     6721 acttgacatt ggaaggcttg attacccggc aagatgtaat gaaagcgatg cagcttgtcc
     6781 agcgacaacc gcagattgca gatacgattt cggatcagat atctggaaat atccatacga
     6841 ttgatactga ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc gtagtagcac
     6901 cacaaatggt caatggcgtt ggaaccattt cttttggcgt acttagcgaa atcatttctg
     6961 atgtcgcaca aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt gaacaagtga
     7021 acctacatta tttgcggctg atccaattgg aaagcgaatt agacattcgt ccacgtgtat
     7081 tggaaatcgg cagacgttcc gctaaactgg atatcgaagt gtttattgaa aacaccatcg
     7141 tttccaaagc aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca ggagtgaaat
     7201 gacagtacag caagaaatag tagatacaat aaaaaaatac gataaaatca ttatccacag
     7261 gcatttgcgc cctgatcctg atgcattagg atcacaagta ggattagccg aactattacg
     7321 ggaaagtttc ccagaaaaag aaatacgtca agtaggtgaa acgattgaag ggcttcgctt
     7381 tttagcagaa atgcaagaag ttgaagatgt cttctaccaa ggagcgctag tcatcgtgac
     7441 tgatacagct aattcaccca gaatcagtga tcaaagatat cagttggcag aaaaactgat
     7501 caaaatcgac catcatccca atgatgaccc ttatggagat cttgtgtggg taaatacaaa
     7561 agcaagtagc tgtagtgaga tgattgcaga atttgctttg atgtttcctg aatctttaaa
     7621 gatgaacgtt aaggctgcac ggttacttta tgcaggaatc gtcggagata cagggctctt
     7681 tttgtatccc tccacgacgt cccgtacatt agagattgct tcgatattga gaaattatcc
     7741 atttgatgca agtgctttga acagagagat tgaacaaatg ccgatgaaag tcgctaaact
     7801 ttccggttat ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa gaataatcct
     7861 tccgcaagaa attcttgata aatatggaat cgatgattca gagacagctt cgatcgtttc
     7921 acttcctgga gtgattgacg aagtgctggc ttgggggatt tttgttcagc agccttcagg
     7981 gtattaccgc gtacgtttac gttctaaggg gccgatcatc aatgaattgg ctaaacgcca
     8041 tcatggagga ggccatccat tagcaagtgg tgcgaatgca aaagatttag cagaagtaga
     8101 ggaaatctat aaagagatcc aagatatatg ccaatcgtat aagagcagaa aaacagaatg
     8161 atacttcgtt cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct tttcaagatc
     8221 ttctttttcc gttacaatag tgctcactaa agattttaaa ggagaaagaa gatgacatta
     8281 ccaaattgcc cagaatgtgg ctcagagtac acatatgaag atcgaggctt gttgatttgc
     8341 ccagaatgcg ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga agacggatta
     8401 gtggtattag atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac agtgattaaa
     8461 gacttgaaag taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt caagaacatc
     8521 cgtttagtag aaggcgatca taatatcgat tgcaaggtag aaggatttgg accgatgaaa
     8581 ctgaaatcgg aatttgtgaa aaagaattaa agaatattca aggtcttttc acattctttt
     8641 cctactacta atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat gtatggttat
     8701 ggaatgtatg gttttttaga tccgacattt cttttggtca ttcttggatt agttatttcc
     8761 ggaattgcgt cagcgtacgt caatagtact ttccgaaaat acgatcaagt gagaagcaaa
     8821 aacaatgtga ctggtacaca agcagcacaa tatatattac aaagtcaaca gataaataat
     8881 gtcggtgttc aacaaattgc cggagatctc acagataact acaattcagg taataagatg
     8941 ttgagtttgt ctcaagcaac tgcacagtct acttcagttg ctgcgatagg tgtagcagct
     9001 catgaatgcg gacatgcggt acaagatgct tcgggttatg tcccattgaa gattcgtgca
     9061 gcacttgttc cagcagctaa ctttggttcg atgatttcgt tccccttgat tatggtaggt
     9121 gtattgctta gctggaactc tacgttaatc aatatcggga ttttcgcttt ttctttagca
     9181 ttacttttcc aattggtgac attaccagtc gaatttaacg cgtctcggcg agcaatccaa
     9241 attttgagtg aaggcggact actgacggaa gaagaagtac cgatggcaag acacgtatta
     9301 tttgcagcag cactgactta tgttgcagca gcgctttcga cattcctgca gctgttgcgc
     9361 ttgatcttat tgttcggcgg caaccgaaga gattaaaata aagaagttgt gaaatcagcg
     9421 ctttgatctg agctaatacc gagagttagc tgattgaata ccatttattt ggaataagag
     9481 gatgtgacaa gaattttgtc acatcctctt tgttttgttc tcataacctt ttttcatata
     9541 cagtttatga accgctttta ttaacttgcc aaataacact gtattgtgcg ttgtatccag
     9601 atagaaagtc tccgtttcat ctgaaatttt ctcatgctga tctgtaatga tcaaatcagc
     9661 atgttctgaa tatgcggtaa ttttaacagc atctttgcga aataactgct gcaaatggct
     9721 ttgaagatga taatttcctg taaactgttt ggttatctgc aaatggatga gtaactgctc
     9781 tttcgtttct gtttgtagta agtgttggat caattgacta aggtattgac tgatttggtt
     9841 tctttcccca ttttttgaaa aaattgtatc gattttttct cttaatttct tttcctgtat
     9901 aaaagaacta gaatgattgg acgtggcttc tggaaagcaa agtgtagaaa aactactgct
     9961 ggattcaccc aacagttcga aaaaaacaac tgccaacaca agaaaataaa aataaattgg
    10021 tattttattt tctaaatggt tgagaggaaa ctgttgagtt aagtaagtca tcaattgacc
    10081 gacttgtcga caaataaggt ttttgcttgt ggaaaataaa aaaccaatct cctgtttttg
    10141 tcgaaaagag atttgatttg gaaaaaaaca atagaaaaaa taattaaagt aggctctctc
    10201 tgtttctgtc tcttcacaag ggcgactcac tgaaaaagaa gtaaagtatt ctgaataatc
    10261 atgtgtatgc tgcaataagt ccatcaattc agtgatttca gaagaagttt ttggaaaata
    10321 tgcctgttgt ttgtttcgta atatgaataa agttaacagc aagtagatat ttttttcttg
    10381 ttttgaaatt ttttctattt gctcatctgt actgttcatt tttagtgcat ttaattcaag
    10441 tgaggaaaat ggccaatcgt tacctccaaa tgtatctgaa agaaaaaagt aaagatagag
    10501 acggatagat aactcatttc cttcaagagt acagcaattt tttacagata ggatccgtaa
    10561 atggaatttt tttaaggaat gattgagctg ttttgtcagt ttttcgagat aggagggcga
    10621 tatataaagt tgttcaagta aatatttttt tggtacagca gtttgtggat aattgattag
    10681 taacatcaaa tagttaaaac gattggattc ttttaggtag cataatttta acttagaaaa
    10741 aacagctaac tttttagttt gatcggtttc tttggtttgg atctctcctt tatgattaga
    10801 ataaataaat tcatcaatca ataaaaattg cttgagtgat acatttattt ttcggatcag
    10861 ttctttggtt gtccgttttg gaatatgtaa gtcattggat aattcttgta aagaacaatt
    10921 atttttttga atgaccattt gtagaacaag taactctttt tttcttgctt caattagcat
    10981 aaatacctcc tttttattct gtttttgtat atagttagac aaaaactgtg agtggaaagt
    11041 catttcagtc tgtgt
//
LOCUS       DAHEYX010000089        10942 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHEYX010000089 DAHEYX010000000
VERSION     DAHEYX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.090"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(373..597)
                     /locus_tag="MB219_001563"
     CDS             complement(373..597)
                     /locus_tag="MB219_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427282.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            788..3565
                     /locus_tag="MB219_001564"
     CDS             788..3565
                     /locus_tag="MB219_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCC8427283.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3646..4065
                     /locus_tag="MB219_001565"
     CDS             3646..4065
                     /locus_tag="MB219_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCC8427284.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4077..4547
                     /locus_tag="MB219_001566"
     CDS             4077..4547
                     /locus_tag="MB219_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8427285.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4564..5343
                     /locus_tag="MB219_001567"
     CDS             4564..5343
                     /locus_tag="MB219_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8427286.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5330..6151
                     /locus_tag="MB219_001568"
     CDS             5330..6151
                     /locus_tag="MB219_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCC8427287.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6168..7217
                     /locus_tag="MB219_001569"
     CDS             6168..7217
                     /locus_tag="MB219_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCC8427288.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7230..8234
                     /locus_tag="MB219_001570"
     CDS             7230..8234
                     /locus_tag="MB219_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCC8427289.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8867..9886
                     /locus_tag="MB219_001571"
     CDS             8867..9886
                     /locus_tag="MB219_001571"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCC8427290.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            9929..10810
                     /gene="galU"
                     /locus_tag="MB219_001572"
     CDS             9929..10810
                     /gene="galU"
                     /locus_tag="MB219_001572"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCC8427291.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         3690 a         1815 c         2314 g         3123 t
ORIGIN      
        1 ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca
       61 gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg
      121 agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg
      181 ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg
      241 acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat
      301 aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga
      361 aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat
      421 gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc
      481 tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat
      541 ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga
      601 atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa
      661 ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct ataataagat
      721 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
      781 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
      841 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
      901 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
      961 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
     1021 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
     1081 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
     1141 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
     1201 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
     1261 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
     1321 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
     1381 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
     1441 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
     1501 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
     1561 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
     1621 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
     1681 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
     1741 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
     1801 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
     1861 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
     1921 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
     1981 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
     2041 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
     2101 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
     2161 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
     2221 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
     2281 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
     2341 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
     2401 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
     2461 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
     2521 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
     2581 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
     2641 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
     2701 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
     2761 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
     2821 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
     2881 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
     2941 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
     3001 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
     3061 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
     3121 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
     3181 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
     3241 agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
     3301 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
     3361 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
     3421 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
     3481 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
     3541 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
     3601 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
     3661 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
     3721 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
     3781 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
     3841 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
     3901 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
     3961 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
     4021 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
     4081 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
     4141 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
     4201 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
     4261 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
     4321 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
     4381 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
     4441 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
     4501 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
     4561 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
     4621 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
     4681 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
     4741 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
     4801 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
     4861 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
     4921 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
     4981 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
     5041 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
     5101 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
     5161 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
     5221 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
     5281 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
     5341 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
     5401 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
     5461 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
     5521 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
     5581 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
     5641 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
     5701 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
     5761 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
     5821 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
     5881 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
     5941 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
     6001 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
     6061 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
     6121 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
     6181 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
     6241 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
     6301 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
     6361 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
     6421 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
     6481 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
     6541 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
     6601 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
     6661 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
     6721 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
     6781 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
     6841 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
     6901 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
     6961 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
     7021 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
     7081 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
     7141 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
     7201 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
     7261 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
     7321 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
     7381 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
     7441 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
     7501 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
     7561 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
     7621 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
     7681 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
     7741 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
     7801 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
     7861 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
     7921 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
     7981 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
     8041 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
     8101 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
     8161 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
     8221 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
     8281 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
     8341 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
     8401 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
     8461 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
     8521 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
     8581 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
     8641 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
     8701 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
     8761 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
     8821 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
     8881 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
     8941 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
     9001 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
     9061 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
     9121 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
     9181 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
     9241 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
     9301 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
     9361 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
     9421 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
     9481 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
     9541 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
     9601 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
     9661 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
     9721 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
     9781 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
     9841 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
     9901 gttaatcgtc gtttaggagg aacagcaagt gaaggtcaaa aaagcagtca ttcctgccgc
     9961 tgggctagga acacgttttt tgccggcaac aaaagccatg gcaaaagaaa tgcttccaat
    10021 cgtggataaa ccaacgatcc agtttattgt ggaagaagca ttggattcgg gaatcgaaga
    10081 catcttgatc gtaacgggaa aagaaaaaag acccattgaa gatcactttg atgcaaatat
    10141 cgaattggaa aacaatttgc gggaaaagga aaaaacagaa ttactcgctt tagttgaaga
    10201 aacgactcaa gtcaatcttc acttcatccg tcaatctcat ccaagaggac tgggggatgc
    10261 cgtgcttcaa gcaaaagcat ttataggaaa tgaaccgttt gtcgtcatgc tgggggatga
    10321 tttgatgaaa gatgatatcc cattgactca acaattgatg atggattatg aagaaacaca
    10381 agcttccgct gttgccgtca tgcgtgtacc agaaaatgaa acatctaaat acggaattat
    10441 tgatcctaaa acagatagcg aaaagggtcg ttgcagagta aaagggtttg tagaaaaacc
    10501 tgaaatggga aaagcaccaa gtaatttagc cattatcgga cgttatctac tgactcctaa
    10561 aatatttgag atattagaaa ctcaagaacc aggagcagga aacgagatac agctaacgga
    10621 tgcacttcag actcttaacc agacagaggc ggtctatgcg agagagttta aaggaaaaag
    10681 atacgacgta ggcgataagt taggatatat gaagacaaat attgaatatg gacttcaaca
    10741 ttcggaaatt gggacttcat taagtgcata tattatccag ttgagtaaaa agctaagaac
    10801 agaaagttaa ctggaaataa gtggcctgct gagaatgaaa ggctatttgt tattaaagcg
    10861 gcagcttgtg cgtcatttgc gcaactgtcg ttttagtttt ttgactctag atcagttgag
    10921 tcgtggaaaa cgaaagatag aa
//
LOCUS       DAHEYX010000090        10869 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHEYX010000090 DAHEYX010000000
VERSION     DAHEYX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.091"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            34..612
                     /locus_tag="MB219_001573"
     CDS             34..612
                     /locus_tag="MB219_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCC8427292.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            612..1541
                     /locus_tag="MB219_001574"
     CDS             612..1541
                     /locus_tag="MB219_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8427293.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1538..3274
                     /locus_tag="MB219_001575"
     CDS             1538..3274
                     /locus_tag="MB219_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCC8427294.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3337..4173)
                     /gene="pheA"
                     /locus_tag="MB219_001576"
     CDS             complement(3337..4173)
                     /gene="pheA"
                     /locus_tag="MB219_001576"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCC8427295.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4170..4679)
                     /locus_tag="MB219_001577"
     CDS             complement(4170..4679)
                     /locus_tag="MB219_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCC8427296.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4672..5967)
                     /gene="aroA"
                     /locus_tag="MB219_001578"
     CDS             complement(4672..5967)
                     /gene="aroA"
                     /locus_tag="MB219_001578"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCC8427297.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5980..7071)
                     /locus_tag="MB219_001579"
     CDS             complement(5980..7071)
                     /locus_tag="MB219_001579"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCC8427298.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7088..8254)
                     /gene="aroC"
                     /locus_tag="MB219_001580"
     CDS             complement(7088..8254)
                     /gene="aroC"
                     /locus_tag="MB219_001580"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCC8427299.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8256..9320)
                     /gene="aroB"
                     /locus_tag="MB219_001581"
     CDS             complement(8256..9320)
                     /gene="aroB"
                     /locus_tag="MB219_001581"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCC8427300.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9353..10375)
                     /gene="aroF"
                     /locus_tag="MB219_001582"
     CDS             complement(9353..10375)
                     /gene="aroF"
                     /locus_tag="MB219_001582"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCC8427301.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         3018 a         2397 c         1998 g         3456 t
ORIGIN      
        1 aattacaaga ctattcttaa ggcgggtaaa aaaatgccaa tacgcgtaat tcttatcgat
       61 gatgaacaga cgattttaga tggaatgaaa cagttgatca attggtctga gcacgggttt
      121 caattaatag ggactttttc cagtccgctg aaagcgcttg atttttcttt ggtacatgca
      181 atcgatatcg tggtcacaga tgtaatgatg cctgaattaa atggaatcga attatcgcgg
      241 ctattaaaac agcaacaacc tgaaacacag atccttatcc tctctagcta tgatgaattt
      301 gatatggtta aagattcttt taaagaaggc gtcgctgatt atcttttaaa gcctcgattg
      361 agtcctgatt tttttctaaa ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc
      421 aacaaaagcc caatcaccaa tcgttatgag cagatctgcg agaatctttc acagcagatt
      481 gctggacaaa ctcgtccatt gcttgaccct cttctttttg aacacacctt gtttttctgt
      541 tatataccga aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca
      601 cccattcatt gatggatagc gacgattcgt ttactattta tcctgtttct accttttcgg
      661 aagaatccgg ctatgtaatc aatactgatt ccaaagatac acttaggaag tttgttcaaa
      721 agtttcgtca agaagttttt ccaggtgatt tttttgttgt ttctgattct tttgcaatcc
      781 aagatatcaa tgaaatattc caagagatca gaaattgcac caaaggacaa cgtttctatc
      841 ataagggaaa gaaccttgct tatcagcatg aacttcttcc gttattaacc aaccacgagc
      901 agcaagcaag tagttattta acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag
      961 aacttgagaa tttcttcacg acgatattgg aacagcctat tgatccagct tatttgaaac
     1021 atgaggcaat caatcgtttc tatgcattac tcaacgcttt ggctgacgag catgttggga
     1081 atgaagaact ttctttgctc aagatcgccc ttccttcttt attagccgat gctgagtatt
     1141 tcgaagattt ttctattctt gtcaaagaaa cacttgagaa actcacccac ttttctgcaa
     1201 atgcaaaaaa agacaatttc gatatcttga ctttgatcta cgagttcgtc caggaaaatt
     1261 atgagaaaga aatcaattta caacttttat ccgaaaaata tcatttttca tatagctatc
     1321 tctcagccat ctttactgaa aaatttggta tcaacttttc aaaatatcta aaaaaagtcc
     1381 gtatcagtaa agccaagctc tttttgacgg aaacacggct tactttgacg gaaatctgtg
     1441 agaaagtcgg ctataccgaa ctgggttatt tctccagagt ttttaaagaa gagacaggaa
     1501 tgacaccttc tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca
     1561 tgaactcttt gccaaacttc tgttcatttt gttcatcggt ctttttctgc aagccatcgt
     1621 catttctttg tttatttatc atcgatcaag ggatgcctat atccagctgt tcaatcaatc
     1681 gaatgatgtg gtactaaaaa agattcaatc tgaatttgaa agtttgaatg atacaatcga
     1741 gaatacatta gccgcttttg attctaatcc agctgtcaaa agctattttt ccaatgatcc
     1801 agcccaacat atggaacagc ttcagcaact aagaacaatc caaaaaatga atgactctct
     1861 ttcaaaaatc catccaatga tcgattacga tgtattgatc tttggagaaa atggaagaac
     1921 ttttgtcgga aacgacatgc tcactgccgt atctgccgat tctttttttc aatctgcgat
     1981 tgctcagaga gtgaatgaac gtgctgctga tacacaaatg ctttttgcac accacggatt
     2041 gactcttcgc gataagaaag cacccagcgt cttctttgtc aaaaaactga aaaatacatt
     2101 gaatcacgtt tacggatatg ctgttttatc gatctcatcc aagcaactag cgaatctttt
     2161 tcaatctgta gtaaatcctg agatatcgaa gatctccttc atgaacgaag aacagacgat
     2221 catcgcatca aatgaagaaa atacaattgg gagccgatca atgattttca aaaaactaac
     2281 agtaggtgaa acaacgattt caaatggaca gcagttgacc cgtcttcctc tgtaccgcca
     2341 ggattgtaca ttactcagtc aagtcgatct tcattcttta tctaaccaaa tgggcgtgat
     2401 ttttccgatt attttgtata atatcgtgac acttgctttt gctggctcat tggtatttat
     2461 gtatttggat cgacacacaa aatcgatcta ccgcttgatt gattcgctaa aacggctaga
     2521 ggaagatggc ttaaaagcta ctgttcctgt acaaggaagc catgaagtcc aattgctagg
     2581 gaatacgatc aatcaattgc taagtaatgc tcagcatcat tatgaacaga cgatccaaga
     2641 cgaaaagaag aaacgttcgc ttgaaatcca aactatgcaa gcacagatac agccccattt
     2701 tatttataat acgctgacta gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc
     2761 agttcaagga atcgacagtt tcattacttt actaagaagt acgattggga aaaaagaaga
     2821 agtgattcca gtcaaggaag aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat
     2881 gcgatatggc gaaagaatct cggctaaaat aatgattcct gatgaactgc agtcattatc
     2941 tatgccgaat atgatccttc aaccaattat cgaaaacgcc tatcttcatg cttttcacaa
     3001 taaaacgcaa ggattcatca cggtgtacgg caaaacaaaa aatcagcttc ttttattcga
     3061 agtgattgat aacggcgatg gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt
     3121 ttcaggaatc ggcatcagcc atgtagatga gcggatacgc ctaatgtatg gcgatgcata
     3181 tggcttaacg atccaatcag tgattgggac aggaacgact gtaacgattt gtctaccgct
     3241 tcttcctaaa aatcaatcag aatatcaaaa ataaagaata gccaagcgct gattttttta
     3301 ttttaagtta aataaatcaa tcagtattct tgactttcat ctacgcgtct cttctactgt
     3361 cagcactgga taacagccca atatttgact ttctcctccg agcatcttga tttcttggag
     3421 cgcattctct actaatgtca ttggacggtc caacaacaaa tcgatcacga aaaaatattc
     3481 tcccaagcta gttttcaatg gtcgggattc aatcttactt aagttgattt cccgccagcc
     3541 aaaagctgcc agcattttat gaagcatccc tggccgatta gcaggtagtg tcaagatgac
     3601 actcattttt actggagctg actgctggct ggtcatcttt cggtcaccga cgatccaaaa
     3661 acgtgtctgg ttcaactcat tatcttggat attctcagct aagatctcca atccaacatg
     3721 ctgtgctgtt tctaatgaag caatcgctgc tgcgtcttct ttcggatgtt ctgctacata
     3781 cattgctgca gcagttgttg attctgttgc tactaaagga acattaggat aatgcgtttc
     3841 taaaaactgt tgtgattgtg ctagtgcttg tggatgtgaa aggatttttg taatctttgt
     3901 tgctggattg cctaaaagct gctgcttgat aggaagaacg atttcacttt taacttcgac
     3961 ttctggatgt ttggaaagaa gatcgattgt gtgatgaaca gctccttcca atgaattctc
     4021 tacaggaacg acagccagat cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat
     4081 ggaagcatac gcagtcaatt gttctgtagg aaatagctga caagccgcct gataagtgaa
     4141 tgaactttct ggccctagat agctgacgtt catatgcttt tgatcctttc tataatggta
     4201 tcgatgatct tttctggtgt ttgattggtc gtgttgattc gatgtgtcgc actttcttca
     4261 taccaagaaa gtcgttgtgc caataatgct tttatttctt ctcttgtttt tccaagagct
     4321 aaaggtcgaa tagacgtttc atcttgttgg atacgatcaa ctagacagtc cgcttctgcc
     4381 tctaagtaaa gaacaatcgg ctgtgttttt aaaaaacgcc gattttctgc actttggaca
     4441 atccctccac cagtcgcgat aattttgtct tcatttgaca gctttcttaa aatatctgac
     4501 tccacttttc gaaagctttt ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg
     4561 atatgttcct caatctttgt atctaaatct acatactctt tttgtaaaca ttgagctaac
     4621 cctttaccga ttgttgtttt tccagcgccc atgaaaccaa tcagcaaaat gctagccatg
     4681 agcttctcct cctgacacaa gttctgctaa atcgtcaaaa aatgccggat aagagattga
     4741 aacagcttca gctccttcta gttcgcaagg tccatcagct atcaaagcag ctacttgcag
     4801 catcataccg attcgatgat cgcctctgct ggacactttt gcgccatgca gaggtgtcgg
     4861 cccatggatg atcagaccgt ctgctgtcgc ttttatcttt gctcccattt tctgtaattc
     4921 ttctgccacc gcatcaattc tgttggtttc ttttactttt aattcttctg cgtctttgat
     4981 gatcgtgatt ccttctgctt gagtagccac taaagctaga atgggcaatt catcaatcag
     5041 acgcggaatg atttctccac caatcgttgc tttcttcaaa tgactcgtct tgacacttaa
     5101 atcagccgat tggttctgtt cattgtgatt cgtctgggta atcgctgcgc ccatctcctc
     5161 caacacatct aagataccag ttcttgttgg attgaccccg acatttttta acaacagttg
     5221 gctttccgga acaagaagtc ctgctgctag aaagaaagca gctgatgaaa tatcacctgg
     5281 aacagttatc tgttgaccag ttaatttttg cggtccggtc actatgatcg ttttatcttc
     5341 cactgtgatc cttccgccaa attgacgaat catttcttcc gtatggttac gggaacgttc
     5401 tttttcaacg attacggatg tgccttctgc ttgtaatgcc gcaaacagca aagcagattt
     5461 gacttgcgca ctggctacag gcatattgta tcggataggt gataaagagc gggttcctgt
     5521 cacagtgatt ggtagatact ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg
     5581 taaaggttcc atcactcgcc ccattggacg tttagacaac gaagcatccc cgaacagagt
     5641 agtcgtaaaa ggctgtccag ccaaaatacc catcaataaa cgtatggtcg taccggagtt
     5701 tcccatgtca attgattcgg ttggttggac aaatgagtgg ctaccgcgac catgaatctt
     5761 gatcacctct tcctcttctt cgatctcgac acctaattgt tggaaggcat gtaaagtact
     5821 cagacaatct tctgcgcgca aaaaattttg tatagtcgtc gttccttcag caattgctcc
     5881 aaacatgatg ctgcgatggg aaattgattt atctgctggt atcctaacag tccctcttaa
     5941 tccatgtatt tgttgtaata attgcattta tccagcctcc tatttgatcc agcaatgata
     6001 actggtatta gacataatga ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact
     6061 gatttgcagt attcctaaaa tatcttccct ggtctcttgg atcttcaaat taatcaaaga
     6121 aactcccgct tttccgatca gtccagttac ttctgcaata acgccaggtt catctggcac
     6181 atcgataaat aaatcaaaaa aggcaggaat cgcgccttgc ttatggacag gcaactggtc
     6241 tcgactttct tttgcccgat tgaaaaaaga aaagatttcc gattggttct cagtcatgat
     6301 ccattctgct atttgtgtca tttcttgttg ccaatctgcc aatcgttcca ataatatctg
     6361 cttattgctc aacaaaatat ctgtccacat gacaggatct gaggaagcga tcctagtgat
     6421 atctcgaaag cctcctgctg ccagttgttt ggctcgcgga tactcttgat tgaattgttc
     6481 tgcctgattg actaagccag ctgccagaat atgtggaaaa tgacttagca ttcctgttat
     6541 acggtcatgt tctcttgctg taagagttac atattttgct ttggttccgc aaaatagttc
     6601 ttgcagtttt tcaacatctt ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt
     6661 ttcaaatagt ttagcatctg ctgcctgtat acctgattta tgagaaccag ccattggatg
     6721 tccaccgaca aaccgaagat ttttctgctc agcggatgtc acgacttgct gcttcgtact
     6781 gctgacatcc gtaatgatga cgtgatcaga caagttgatt ctttcaagga catcaagata
     6841 agctaaggtt gtacggacgg gaacggctaa taagataacg tctgcttgtt ctgcacctgt
     6901 ttcaaaatca cttgcaacgt aatcaacgat ctgctgttct actgcttgct cacgggtttt
     6961 tgactgacta tcccaaccaa ctactatcag ttctggattg gtctgacgaa tacaaagtgc
     7021 catagaagca ccaatcagtc caagccccac gataaaaaca tgttgtgcca tcttatttgc
     7081 ccccttttta gtaatgtttc aagtagtcac ggtactcctt cactgcagct ttcaactcaa
     7141 caagcgaatc acttgggaat tttgacaata gctctgtcgc taccacagtg gctacaacat
     7201 tttccgcaac gacgctagct gcaggaactg ctgtactatc cgaacgttct acacttgctt
     7261 tgtaaggttc ttttgtatcg atatcaacac tttgaagtgg cttgtacaat gtaggaatgg
     7321 gtttcatgac cccgcgaatg acaagtggtt cgccattggt cattccgcct tcgaagccgc
     7381 ctagattatt ggtacgtcgg aaatagcctc tttcttcttc ccataggatc tcatccatca
     7441 cttctgagcc tggtaatgaa ccagaagtga atccgacccc aaattctacg cctttaaagg
     7501 cattgatact aaccacagct tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt
     7561 agcttcctaa tccagctgga acgcctccaa cgagcacttc tacaattcct ccgatcgtat
     7621 ctccattttt ctttgtctga tcgatcaaat cttttattct ttgctcaact gctggatcaa
     7681 caactcgtac ttctgacgct tcagaaattt ctttgatttc ttgaatcgag atatttccag
     7741 gtatttctgc ttggattcca ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt
     7801 cttccagaat tttcttggca attgcgccga ttgctacacg catggtcgtc tctcttgcag
     7861 aggaccgttc aagcacattc cgaagatctc gatgatggta tttcataccg cctaccaaat
     7921 ccgcgtgtcc tggacgcgga cgtgcaactc gccgcagtga ttggtctttt tcaggcactt
     7981 ctgctacaga catgacagtt gtccaatttt tccaatcttt attttctacc actaaagtga
     8041 caggtgaacc aattgttttg ccgtgacgga tgccagacgt gatccgaaca cgatcttttt
     8101 cgatcttcat ccttcctcct ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga
     8161 tttcttcttc tgacagaggt aatcctgctg gtagtccttc aataatggct gttaattctg
     8221 gtccatggga ttctcccgca gtcatataac gcatcttaca gtcctcctaa gtaatctttg
     8281 aatgtctctg tgggaacagg aacgattttt gcttgcccaa tcttttctag caagatgata
     8341 ttcaattggc ttcctcttgc tttcttatca tgggttatcg cgtggaataa ttcatctgga
     8401 tgacttagat cgtacgtaat cggcagatgg aatttttgaa gtatcctttc taaagcagct
     8461 gtcgatccag ttggagacaa gccgattttt tctgcatgag aagtgatggc taccattcca
     8521 atagctactc cttctccatg actgactacc ccatatcctg ctgtattttc aattgcatgg
     8581 ccaattgtat gaccaaaatt taaaataagt cgatttcctt ggtcaaagac atcttcttcc
     8641 actacttttc gtttgatttc caacgtagca gtaatgattg gaacagcatg ttccagaaga
     8701 tctttttcgt ctttcagctc gttcagctgt ttccaaagcg ttgcatctgc tatagctgct
     8761 gatttgatga tttcagcgat tccttctcgt aaacgacgta ccggcaaagt ttttagaacc
     8821 tcaggatcaa tcaatacacc agctggctgg ctaaatgttc cgacaaggtt tttcgctttg
     8881 tttgtattga ctgctgtctt cccgccaata ctagaatcta cttgtgcaag tagcgtagtc
     8941 ggaatttgaa cgaaatggat cccacgcatg aacgtcgagg ccacgaatgc tgctagatca
     9001 cccaccattc ctccaccaag tgcaagtatc ccatcgctgc gggtaaaatc attatctgct
     9061 aaaaaatcgt ataactcagc tgcttgttct aaagatttac tggtctcacc cgccggtacg
     9121 attgaaagga ctggttcaaa attagcttgt ctcaattgga acatgatttt ttctgcatat
     9181 agtggagcaa cgtttgtatc agtgatcaca gctacttttt gaggtctcca aatcgtttga
     9241 agccatgatc cagcctgttc tagtgcgcct ttttcaatat gaatatcgta tttttggtta
     9301 tcaacatgta cagtaagcat tcgtttcact tctttctttt aaaaagctgg aattaatcga
     9361 gtgtctgaat cttttccatt gcttctttca tgatcgcaac ttctttcatc atgcgtgagt
     9421 aagtcttttc attcaacgat tggggaccgt ctgaccaagc atttgcagga tcaggatgga
     9481 tctctacgat catgccatct gctccgcttg ccacacccgc acgagccatt ggtggtacaa
     9541 gttcccaaat tcctgtacca tgacttggat caacaatcac agggaaatga gttaattttt
     9601 tcatcaatgg gacagcactt aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa
     9661 tcccacgttc gataaaaatg acaggagatt tgtcttcgac tgcaatgtat tctgcagcat
     9721 taagccattc attgatcgta ccggaaattc cacgtttcaa tccaacaggt ttacctgttt
     9781 taccgacagc agatagcagt ttaaaatttt gcatattacg tgcgccaatc tgtaggatat
     9841 cgctgtactc agccaccata tcgatatgcc cttcatccat cacttcagta atcactttca
     9901 ttccaaattc atcagccgct tgacggatgt atttcaatcc ttcttcttct aaaccttgga
     9961 aagcatatgg agaagtacgt ggtttgaagg caccacctcg cagaattttt gcgcccccgg
    10021 ctttggccat tcgcgcacat tctctgattt ggtctaatcc ttcgatagaa caaggaccag
    10081 ccatcgtcac gaaactgccg tcaccaattt ttactccgtc tacatccacg actgtattgc
    10141 caggatggaa ttctcgagaa gtcaatttgt atgtgtttaa aatacgcaca gcattttcta
    10201 ctccttcata gcgtgtaaat gctccttctt gtaaacttcg cgtatcacct tttagtccaa
    10261 tcactactcg ttctgttcct tgatcgattg agactttgaa tccttctttt tcgatatgat
    10321 cgacgacttt ttttatttgt tcttccgttg cttctttttt cataattact atcattgttt
    10381 ttcccgcttt cttttgttta cttatctatc ttggctgatt ttctctactt tttcaaggtg
    10441 gttttacatt aattcctact aaatgaccgt gtcctgttgt tctcattttc gctccttctt
    10501 tcctacttat gacccagata atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac
    10561 agtgaacatc ttcaacgagc cttaaaaagc gctgcaaaaa tataaaatag ccactataga
    10621 tgaatttctc tacgtggctg taagtcgtgt ttaaaaatct ttagccatca tagatacaaa
    10681 cctggtttgt ttgtacccac gaagtcatga atacaaacct atctaaaata gctaaagtaa
    10741 tacgtattca gttttgtaag ttttctagat ttcataaaaa atcgctccaa actgatgtaa
    10801 gaattgtacc tagtatagac ataaaaaaat cgactgtcaa tcatttttat gaaacttttt
    10861 ttaattttc
//
LOCUS       DAHEYX010000091        10773 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHEYX010000091 DAHEYX010000000
VERSION     DAHEYX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.092"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..2315
                     /locus_tag="MB219_001583"
     CDS             <1..2315
                     /locus_tag="MB219_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCC8427302.1"
                     /translation="RRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAALGIGIGAGYFA
                     YLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTISSDKISPLLKT
                     AIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQQILTDETTFKR
                     KANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAKGIFGVDAKDVT
                     LPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYREGQITKEQYEEA
                     KAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADMSDSSVSDAYYQ
                     KAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETGNVLMDNRTGRI
                     YGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSESRVSDYATTWQE
                     GENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGSKQYAYEHYLAK
                     MNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDSITDNAGNVIYK
                     HESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKADWVGKTGSTNE
                     YRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAYQAKPDIFGTDE
                     KFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGPKKSAFKFGVGG
                     TDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(2652..3653)
                     /gene="ccpA"
                     /locus_tag="MB219_001584"
     CDS             complement(2652..3653)
                     /gene="ccpA"
                     /locus_tag="MB219_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCC8427303.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            3863..4969
                     /locus_tag="MB219_001585"
     CDS             3863..4969
                     /locus_tag="MB219_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCC8427304.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            complement(5028..5630)
                     /locus_tag="MB219_001586"
     CDS             complement(5028..5630)
                     /locus_tag="MB219_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCC8427305.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(5633..6073)
                     /locus_tag="MB219_001587"
     CDS             complement(5633..6073)
                     /locus_tag="MB219_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCC8427306.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(6198..7136)
                     /gene="galU"
                     /locus_tag="MB219_001588"
     CDS             complement(6198..7136)
                     /gene="galU"
                     /locus_tag="MB219_001588"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCC8427307.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(7151..8176)
                     /locus_tag="MB219_001589"
     CDS             complement(7151..8176)
                     /locus_tag="MB219_001589"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCC8427308.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(8220..9050)
                     /locus_tag="MB219_001590"
     CDS             complement(8220..9050)
                     /locus_tag="MB219_001590"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCC8427309.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(9065..10003)
                     /locus_tag="MB219_001591"
     CDS             complement(9065..10003)
                     /locus_tag="MB219_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCC8427310.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(10170..10520)
                     /locus_tag="MB219_001592"
     CDS             complement(10170..10520)
                     /locus_tag="MB219_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCC8427311.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            complement(10520..>10773)
                     /locus_tag="MB219_001593"
     CDS             complement(10520..>10773)
                     /locus_tag="MB219_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCC8427312.1"
                     /translation="EYIGIDPTVIRVLYVFLSLVAFGSPIILYILLALIIPSARSART
                     NYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEWSDF"
BASE COUNT         3179 a         2265 c         1974 g         3355 t
ORIGIN      
        1 atagaagaaa aaaagaaaaa tggtttgttc ctaagattat ttttagagtt tttcaatcat
       61 tgactgtttt tattactgtt cttcttttga tgttcgcagc tctaggtatt ggaataggcg
      121 ctggctattt tgcctatctc gtcgaagaca cacaattgcc gactaaaaaa gcactgcaga
      181 cagaactagg aaacattaca gaaacgtcaa agattgttta tgcagataat acagagatat
      241 caaaaatcca gacagatctg atgcgcacga ctatttcatc tgataagatt tcacctttat
      301 tgaaaactgc aattatcagc acagaggatg aatacttcga taaacaccaa ggttacgttc
      361 ctaaagctgt tttacgagca ttagtatctg aagctactgg tattggctct tctggtggat
      421 caactttgac acagcaatta gtcaaacagc agatccttac agatgaaaca acattcaaac
      481 gtaaagcaaa cgagatcatt ctttctgcac aagtagaaaa atacttttca aaagatgaga
      541 tcattgctac gtatttgaat gtctcaccat ttggtcgtaa taataaggga caaaacattg
      601 ctggagtcca ggaagctgca aaaggaatct ttggtgtaga tgcaaaagat gtgacgctgc
      661 cgcaagctgc ttatatcgct ggattacccc aaagcccgat cacgtatagt ccatacacga
      721 acacaggcgc gctaaaagaa gatctttctg ccggccttgc cagaaaagat tttgttttat
      781 tcagtatgta tcgtgaaggc caaattacga aagaacaata cgaagaagca aaagcctatg
      841 accttacaaa agactttcta ccacaacaaa tcgcagaaca gactgacaga gaattcttgt
      901 attatactgt gatgaatgaa gcaacaagaa tcattgcaca acaattggct gaaaaagaca
      961 acgctgatat gtctgattca tcggtcagtg acgcttatta tcaaaaggca cagcaaacga
     1021 tccaaaataa aggatataca atccattcga caatcgataa agatgtctat gctgcattgc
     1081 aaaatggcgt tgaaaattat ggatacatgt tagatgatgg tgctggcagt caagtagaaa
     1141 ccggaaatgt attgatggat aacagaactg gccgcatcta cggattcgtt ggcggacgga
     1201 attacagtca gaatcaaaat aaccatgcct tcgatacaga acgtcaagct ggctcatcaa
     1261 tcaaaccagt tcttgtttat ggacctgcaa tcgacatggg gcttatcgga tcagaatccc
     1321 gtgtatcaga ttatgcaaca acttggcaag aaggagaaaa tgctggcgag aaaatcgtca
     1381 atgctactaa cgaaggatcg aacaccttcc aaaccgtccg tgagtcacta gaatggtcca
     1441 acaatatccc ggcttatcac ttatatcaag atgtgttgaa ttccggtgga tcgaaacaat
     1501 atgcttacga gcactacttg gcaaagatga actacccggc aaatgacaat tggggcgttg
     1561 aatctgctcc tttaggtaca gtagatgtca cgacgcttca acagaccaac ggatttcagg
     1621 ctttagcaaa cggcggcgtc tatgaagaag gctatatcat cgattccatc acagataacg
     1681 caggaaatgt aatttataaa cacgaaagca actctgtccg gatctattcg gaagcaacgg
     1741 catcgatcat gaatgacatg atgcgtagtg tgatcaatgc aaaaatcact acaccattca
     1801 aagatgccat ctcaagctta aacggaaatc taggaaaagc agattgggtc ggaaaaactg
     1861 gttctaccaa tgaataccga gattcatggc taattgtctc tactccgtct attaccatca
     1921 gtagctgggc agggcacgat gacaatactg gaatggacag caaagcaagg atacgttcgg
     1981 ctaattatct tgccaatctg atcaatcaag cttaccaagc aaaacctgac atttttggca
     2041 ctgatgagaa attcgaatta tcatctgatg tgatgaagaa aaaagtatct ggatttactg
     2101 gtcaaacacc tggaaaagtt actgtcaata aaaaatccat ccaaacacca ggaaaaacag
     2161 ttgaatcgct atgggcgaaa aatggaccga agaaaagcgc ctttaaattt ggcgttggcg
     2221 gtaccgatga aaactataca gattactgga acaccgcttc agcttatgca agagcgaatc
     2281 aagcaactaa agaatcagat gagaaagatg attgatttaa tagaatacac agtaataagg
     2341 gattgtgaca atagtattgt cacagtccct tatttttttg atggtggtga acaaaatgca
     2401 gcatctatac acggattgtt ttatatacga taaatgtaat ttcaaccact ttctctcttt
     2461 tttaagacac aaaaaaagga ttataacaaa attttgtcat aacccctttt cccaaataaa
     2521 cggtgttctc ctgccaactc cgaatcacga gtcacaatat tcatcaaaca tgagatgctg
     2581 ttttttgaaa ttgctccttg taacgcagag ttaaacggca ggcacacaac ctctgaatgg
     2641 ttgtcctttt attattttgt agatcctttt tcttcgattc cataaggcaa gataatcgtt
     2701 ttttcttcga cttcttcttt attcatcatt ttcgtcaata ggcgcataga taccgcccct
     2761 aaatcgtata atggttgtgt gatacttgtt aaacgaggac gagctacttc tgtcagtagt
     2821 gaattgttac ttgtgatgat ttcgaattct tccggtactt tcacgccacg gtctaataaa
     2881 ccatccaaaa taccgatggc taattcatca tctgaaacat agacagctgt tgcaccgcta
     2941 ttatggatcc gatcgactag agatagacca tctttgaagt tgtattgtgc ttcaaagatc
     3001 aatccttcgc tatatggtag gttattttcg cttaatgctt ctttatagcc tttcagacga
     3061 ttttgtccat tgatgggatc gatcaacgca ccactgacaa aagcgatttt tttattgccg
     3121 ctttttgcca atttgtttac tgcatctttt gttgcggata cataatcaat gtttacgcta
     3181 cctacttgtt catctggatc aatagagcca gctaatacaa caggagtttt tgaacgtgaa
     3241 aattctccac ggatttcgtc tgtgatatgg tgacccatga agatcacgcc gtccacttgc
     3301 ttagccagta agttattcaa tacattgact tctttttggt cgttgccatc tgaatttgct
     3361 aagataatat tgtatttgta cattgttgcc acatcatcga taccacgtgc taaagaagca
     3421 aagaacatat tgctgacatc agggatgatt acaccaactg ttgttgtttt cttacttgcc
     3481 aagccacgcg caactgcgtt tggacgataa tccaagcgat cgataacttc caagactttt
     3541 tttcttgttg ctggtttaac gtttggattg ccatttacga cacgtgaaac agtagccatc
     3601 gaaacatttg cctctctcgc tacatcatag atagtgattg tttgcttttc catttgtgtt
     3661 ctcctattca tattttatac gttttctgaa aatacgtctt acatttctaa aatagaatat
     3721 caaataatct caagaattgc aaccgtttta tctctgtttt catgaaaatt tttacttttt
     3781 tcagttttta gaacgttggt cttttaataa atcagtggta caattattga atacaaaatt
     3841 aatttagaaa gtaggttgcc aaatgaataa cgtaaaaatc aatgaactac gtcaatggat
     3901 ggcacaacac caagtcgaat tggcttatat cagcgaccct ggccacatcg cttacttttc
     3961 cggatacgaa agtgatcctc atgagcgtgt actagctctt tttatccctc ttgaacaaga
     4021 tcctttcctt ttcactcctg ctttggaagt tgaagatgct caaaacagta gttgggcgta
     4081 tgatgtccgt ggctatttag acagcgaaaa cccttgggag atcatcggaa aagaacttcg
     4141 tgctcgctat ggtcagcctg gcaatgtagg gattgaaaag aacgccttga ctgtcgagcg
     4201 cttcgatgct atctctgata tcttgacggg aacggattct tacactgatc taacttccat
     4261 catccaacgt ctacaactga tcaaaactct agaggaaaaa gacaaactga tcgaagctgg
     4321 taaatgggcc gatgttgcat ttgagattgg ctttaaagca gtgaaagaag gcgtgactga
     4381 acaagcgatc attgcagaaa ttgaatacca attgaaaaaa caaggtgttt cacaaatgtc
     4441 tttcgacaca ttagtccttg caggagcaaa tgctgcaagc cctcatggta caccaggaaa
     4501 tacgaagatt tcaccgaatg aacttgtgtt attcgatctt ggcgttgttt ggaatggcta
     4561 ttgcagtgat gctactcgta caattgctta tcaaaaacca accgacttcc aagaaaaaat
     4621 ctacaacatc actcttgaag cacagcttgc cgcacaagaa gccgtccgtc caggtgtgac
     4681 agcaggtgaa cttgatcaaa tcgcacgaaa cgtgatcaac agctatggct acggcgaata
     4741 tttcaatcac cgtttaggtc acgggatcgg aacaaccgtc cacgaattcc cttcccttgt
     4801 agaaggaaat gacttagtaa tcgaagaagg gatgtgcttc tctcttgaac caggaatcta
     4861 tattcctgaa aaagtagggg tacgtatcga agactgtgta tatgtgacaa gcgatggctg
     4921 tgtgccattc acgactacac caaaagaatt acttgtttta gaaaaataag agtaaaaaag
     4981 gtgagagcta gtccgctccc acctttttat tctataaatt tgtgatttta gtgatttgta
     5041 gatgaatctg tcattgtatc ttttatttct tttttagctt ctttcgcatc ttcttttgcc
     5101 attttcatca tttcttttga gtcatctgca actggttttg cttctgacat tccttcattg
     5161 acagtatctc ctgctttacc agcagcgtct tttacatcaa tcatgatatc ttctgaagta
     5221 tctttagcaa tacttcctaa ttcatctgca ggttttttca tatcttctgc cgtttttttg
     5281 aaacgattag ataagtcagc tgattgttct ttaagattta acaagatatc gtcagattca
     5341 cttcctgctt catctgctga ttcacttact ttttgtttga cactacctgc tagatcacct
     5401 gcttgttgag aaagatcttg cgctttttct tttgcaatac ctgttaactc agtcgatttt
     5461 tctttcgcca tatctgtgta atctgaagcc atatccatca aatcatccgc ttgggcagct
     5521 aattcatccc gaagttcttt accagatttt ggtgctaaca ataacgcggc gattgctgcg
     5581 gctgtgcctc cgactactgc acctaataaa aatccacctt ttttagccat ttttattttc
     5641 ctcctgtcgt ttcagtatgt tttttgtttt taaaaaattt catcgctgtc ccaccgactt
     5701 ttccggcaac cgtcgcttta gctgtcgttt taccgattcc gccgactttc gtcgcgatgt
     5761 ttttactaga atgattaagt tctgatacac tttcacttaa atcagcaact gctgtgaaca
     5821 atggatcgat cgttgcgact tttccattga tatccaagag cagttcatta cttttgacca
     5881 gtaagccttc tacttgtcta gacaaaagat ctacgtcttt tgtgacgatt tcgatcgtcg
     5941 tatttgcttc tgctacagtc tgatctactt tttctaaagt tttagaaact tgcaacaaca
     6001 cacgaacgat aaaaacgaca agtatcgcaa aggcgattgc tgcgatcaat cctgcaattt
     6061 ctcctcctga catgatcaaa ctcccttctt gtctctcttt tctataagga tagcattttc
     6121 gtgtttttta aacaaataaa aacgagcttc aactactttt tattcaatta gcagctgaag
     6181 ctcgttagaa aaaagggtta ttttttcttt tcttctttga tttctgttat tccttttgga
     6241 ttatgatcga tcttgcttcc ttctgcttct aatgttttac ctaaagacag gatgtactca
     6301 cgtaaaggtg cacctacttc tggatgtgtt agaccatatt caatacttgt tttcataaaa
     6361 ccaaatttgt caccgacatc gtaacgttta cctgtgaatt tacgggcaaa tacgcgttgt
     6421 gttttattca atgtatcgat tgcgtcagtt agttggattt catttccggc tcctggttgt
     6481 tgctcggaca atacatcaaa aatttctgga gtcaataagt aacgtccaat aatagctaaa
     6541 tcacttggtg ctttgcttgg atctggtttt tcaacaaagt ttttgacatt gtataaccca
     6601 tcttccaata cttctcctgg attgataatt ccgtattttg aagtttcatc atgaggaact
     6661 tgcataacgg cgatcgtaga agcatgagta cgttcgtaat catcgattaa ttgctgtgtc
     6721 aatggtactt cgtcttccat tatatcatcg cctagcataa cgacaaatgg ttcatttcca
     6781 ataaaggctc ttgcttgaag aacggcatgt cctaaaccct ttggatgaga ttgacggatg
     6841 aaatgcaagt tgacatctgt cgtttcttct actaatttca ataattcggt ctttcctttt
     6901 tcacttaggt ttgtttctaa ttcaatattt gaatcaaagt gatcttcgat tggtcgttta
     6961 gcttttcctg taacgatcaa gatgtcttcg atacctgatt tcaacgcttc ttcaacgatg
     7021 aactggattg ttggtttatc aacaattggc aacatttcct ttgccatagc ttttgttgct
     7081 ggtaaaaatc ttgttcctaa tcctgctgct ggaataactg cttttttaac tttcatatac
     7141 attcgctcct tcaaaaaatt gttaatgaga attcattttc tttttttcca tcacgtaaca
     7201 tgatttcttt tgcggctgtt ttgatttctt tttgttcata cagcacttca tagattgttt
     7261 gagtgatggg catttcgatt tccatcattt gggctagttc catcgctgct tgggtagtat
     7321 tcacaccttc aacgaccatt cccatattgg ctagcacctc ttctaggggc tgcccttgtc
     7381 ctaacaagtt gcctgcgcgc cagttcctag aatgaacact tgtgcaagta acgactagat
     7441 cgcctattcc gcttaatccg ataaaggtca gcgggttggc acccattgct acacctaaac
     7501 ggctgatctc agctaatccg cgcgtcatga ttgctgcctt tgcattatcg ccaaacccta
     7561 agccgtgaat agcgccagca ccaatagcaa taatattttt caaagctgcc cccatctcta
     7621 caccgatcac atcaggattg gtatagatcc gaaaataatt attcatgaac agctcttgta
     7681 catattttgc tgcttttgca tctgtacttg cagctgtgat cgtagtgatg tcatgttttg
     7741 ctacttcttc tgcatggctt ggtccagaaa gaacaacaac agcttttctt ttttctgctg
     7801 ggatttcctc taacaagatg tcagagatcc ttttatgtgt tccctgttct aatcctttac
     7861 ttgcatggat gatcactggc tttgtattca atttaggcac tagttctttt gcaacagaac
     7921 gtattgcttt cgttggaatg acgaatagca ccgcatctgc gtcttttact gcatcttcca
     7981 tattggtgaa tgcttggatg gaatcgggta gataatgatc tggtaaataa cgttgattcg
     8041 tgtgttgggt gttgatttct tctacttgtg aagcacgatg tccccaaatc cgaacgtcat
     8101 gtccgttttc ggcgaggact tgtgccaaag cagtacccca agagcctggt cctaaaaccg
     8161 ctactctata attcatttta ccactcctga aatttttttc atttttaacg ctctaaaaat
     8221 cagataattt gttgattttt tcctaatgag cggtcataat ccggtaaatt tccttttttt
     8281 ctgcgccaaa taaatagaat caatgtaccg acaaataaca cagcagataa taattgagaa
     8341 atacggatca cacctaaaaa gtaaagactg tctgtacgta atccttcaat aaagaaacgc
     8401 ccgaaactat accacattag ataacataaa aggacttcgc cttttttcaa aaggtgtggt
     8461 ttctttcgta aaaacaaaag caaaataaat ccaatcaaac tccaaagtga ttcataaaga
     8521 aatgttggtt gacgatagat cccttcgata tacatattat caatgatgaa ttgcggtaaa
     8581 tgcagacttt ctaagaagct tcgcgtcgtg tctggtccat atgcttcgtg gttcatgaaa
     8641 tttccccaac gaccgattgc ctgtgctaat aatacacttg gtgcagcgat atctaaaaaa
     8701 gtccacaaag aaataaaatg atatttagta aaaaaataca gcgtgatcgc gcctgcaatc
     8761 agtccgccat aaatagctaa gcctcctgaa cggatattga atatttggat tggatcagct
     8821 atatactggg gaagatcaaa taaaacataa tataagcgag cgccaataat ggagataggc
     8881 aatccccaca acataaaatc tgtcacatca tctgctttta atcctacccg aacagcctct
     8941 ctgctgctaa gccacatagc gataatgata cctgatacga tgatcaatgc ataccagtag
     9001 ataggaatcc ctacaagctc aaaagcaact cgattgattt gtgctaacat attgtcactc
     9061 cttgctagtc gttgcctgag ttttcttcaa tcaagcgact taatctttct tcaaatgttt
     9121 tcgttgcgtc aaagcccatt gtacgtgcac ggaaattcat cgcggctact tcaatgatga
     9181 ttgcaacgtt tcgtcctgtt ttgacaggga tttttatttg tggaatgccc acttccgcga
     9241 tttcaacggt cgtgtcttca gagcccagac ggtcgtattt cttatctttt gcccatgctt
     9301 ccagataaac agccaactgg acttgcatag agccacgtac cgcactcgca ccaaaaagat
     9361 tcattacatc aatgatcccg attcctcgga tttcaatcag atgttccagg attttaggcg
     9421 gttctccgat caccgttaat tcatcttgct gataaacatc tactcgatcg tctgcgatca
     9481 atcgatggcc gcgtttgatc aactccaacg ctgtttcact ttttcctatc ccactgtcac
     9541 cttgaatcag tacacctaat ccgtaaacat ccactaaaac accgtggaca cttgttcgag
     9601 gggcaaggcg accatctaaa taactggata attctcctaa taaacgagaa gttgagatgg
     9661 gagaacgcag tacagctatc ccattttcat ttgctgcctg gatcatttct tctggagctg
     9721 gtaatcctct tgaaataata aatgcgggca tatcttcact acataatctt ctcatgatga
     9781 tcaaacgctc ttctgggatc atccgttccg caaaactgat ttctttactg ccaaatagct
     9841 gtaaacgatt atgggaataa taattaaaat aaccagtcaa ctctagtcct gggcgtgaga
     9901 tgtccccagt tacgatgaca cggtctaagc ttttctcgtc gcctgtcacg atttccaaag
     9961 ataatttttt cactaattgg ctaactttta caacttctga cattcatgct acctcatttc
    10021 tattagagtg ttctctatca ttttatcatt gtctagccaa aagtactacc aattaaaaaa
    10081 aacgttttta tttaattacc aatgtaatag aaaacgcatt cctgattcta aataaaaaaa
    10141 ctgcgccgat agttcggcac agctttcttt tattttctac tgttatgttc gctgacaatt
    10201 acattgacaa gcgacatcaa gatagctact aacaacgctg caccgaatga agaaaaacta
    10261 aaatgttctg ctcccacaaa aaaggaggtc attttaagaa tcgctgcatt gataacaaac
    10321 gtaaacaatc ccaatgtgac taatgtaaag ggcagggaca gtatcgtcaa aatcggtttg
    10381 acaaacatat tcaacacaga caatacgaag ctggcaagaa cggccgtcca aatactgctc
    10441 acatggacca tcgcaggaaa aagaacagcc aatgatatga atgttaacgt attaactaaa
    10501 atacgttgaa agtacgacat taaaagtcac tccattcgtc atcatcgatt ttttccgctt
    10561 ctttgcgttt ttttgtttgt tcacgagttt gttcacgata attgtttttt tcataatatg
    10621 cattgttatg tccatagttt gttcgtgcag aacgcgcaga tgggataatc aaagctaata
    10681 aaatgtataa aataattgga cttccaaaag caactaagct caggaataca taaagcacac
    10741 ggataacagt cggatcaatc ccgatgtatt ctc
//
LOCUS       DAHEYX010000092        10659 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHEYX010000092 DAHEYX010000000
VERSION     DAHEYX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.093"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            52..572
                     /locus_tag="MB219_001594"
                     /pseudo
     CDS             52..572
                     /locus_tag="MB219_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(474..1190)
                     /locus_tag="MB219_001595"
     CDS             complement(474..1190)
                     /locus_tag="MB219_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCC8427313.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1240..1449)
                     /locus_tag="MB219_001596"
     CDS             complement(1240..1449)
                     /locus_tag="MB219_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427314.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1726..2999
                     /locus_tag="MB219_001597"
                     /pseudo
     CDS             1726..2999
                     /locus_tag="MB219_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3106..4137)
                     /locus_tag="MB219_001598"
     CDS             complement(3106..4137)
                     /locus_tag="MB219_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427315.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4144..4974)
                     /locus_tag="MB219_001599"
     CDS             complement(4144..4974)
                     /locus_tag="MB219_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427316.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4971..5786)
                     /locus_tag="MB219_001600"
     CDS             complement(4971..5786)
                     /locus_tag="MB219_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCC8427317.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5783..6607)
                     /locus_tag="MB219_001601"
     CDS             complement(5783..6607)
                     /locus_tag="MB219_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCC8427318.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6597..7697)
                     /locus_tag="MB219_001602"
     CDS             complement(6597..7697)
                     /locus_tag="MB219_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCC8427319.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7875..8660
                     /locus_tag="MB219_001603"
     CDS             7875..8660
                     /locus_tag="MB219_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCC8427320.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8693..9076
                     /locus_tag="MB219_001604"
     CDS             8693..9076
                     /locus_tag="MB219_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCC8427321.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9152..9283
                     /locus_tag="MB219_001605"
     CDS             9152..9283
                     /locus_tag="MB219_001605"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCC8427322.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9252..9488
                     /locus_tag="MB219_001606"
     CDS             9252..9488
                     /locus_tag="MB219_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCC8427323.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9566..10537)
                     /locus_tag="MB219_001607"
     CDS             complement(9566..10537)
                     /locus_tag="MB219_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCC8427324.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3158 a         2490 c         2063 g         2948 t
ORIGIN      
        1 tttggtataa tttagtcgac caaaacatca ccctaccaaa ggagtcgctc tatggataat
       61 catactagaa aattactcgg attaacagat aaacatttat tttttgatga agaatggctg
      121 attgaaaaag aatataaagg cgttcaagcg cagtttatta aagggagatt ggatgactca
      181 ccttcccatt gtgaacactg tggatcgatt cgtattatcc gcaacggttc ctatacgaca
      241 cgtactcaga tgttgaaagt caaagaaaag ttgactattt tagaattaaa acgtacccgt
      301 tttctatgct atgattgtgg gcagaccttt tctgcgaaaa cggacttagt tgatgaacat
      361 catcaactaa caaaggaact gaagcaggcg attctcatag aactgtacga aaatcaatca
      421 cgtaatctga aaatcagaag aagtaccgtc ggctcaagcg atattggaaa ctcttactca
      481 aagattccac gacattggag ccgctcaaac ggcattatca tcgactgttt aaacgaccaa
      541 ttagtcaaac agaaatcgtc gatgaaccgt tgtgcacaat ggtgtcctga acgtacagcg
      601 acaccttcca tatccagagc agtcgccaca tcacggggat ggatgccatc caaattaaaa
      661 gcaaccacgc cagtatgatg ttcaggatct tgcggtccgt aaatggtgat accgccgatc
      721 gcttgtaatt taggtaaaac gtaggcaacc agttcttgct cataacgatg aatattctcc
      781 ataccaatct cattcaagta atcgattgct cctgcaatgt tcggagtacc agcttcaaat
      841 ttccatggca gctctttcca tgaactatcg taaagattga caaaatctat catttcaccg
      901 ccgaattcga ctggctccat ttgttctaac aattgacgtt tgccatataa tacaccgatt
      961 cctgtcggcc cgcacatttt atgcccgcta aaggcgaaaa aatctgcatc caacgcttgt
     1021 acatcaactg tcatatgcgg tgctgcttgg gcaccgactg gactatcctc aactacaaca
     1081 gcctgttcaa ccctcagccg tttcaagcac aacaaataac cttctggatc tggtttgccg
     1141 acctgaagat catcgatcgt cacaaaggaa tcaatttttt ccttaatcat cggatgtgct
     1201 tcaaaaaaat agtctagctc ccgcagttca gaattgagac tactccgatt ttccagtgct
     1261 gctgatccgc tgctcaaaat gttcagcatc cactggtctc ccaaccgctt gacttatccc
     1321 ttcataaaag gacagttctg ctgccgtccc aacaccttgt tgatagtctg caaaaggaat
     1381 tttatagccg aactgctcca gtaaataggc ttcagtaaat agtgatgaat actctcgtaa
     1441 aacatccatt cactgtcaat catcgtccca tcaaaatcaa aaataagtgc tttggtcatc
     1501 ttctttcccc cttacccaaa acaagcaaaa gctgtgtcct gctctctcta cagtatcagc
     1561 ataaaaactg ccgtctgaaa agtcaaaggt gaaagaaatg tggcgctata atttctagga
     1621 ctgatagtaa atcattctat cagtcctttt gagcataaaa aaacaaaaca tacagaagct
     1681 ccgttagaat tgaatcacca caacacaatc aaaggagcgt tctgtatgcc tgatcttcat
     1741 cttatcaaac tattactagg aatcaaagat ggaaacattc aaatcactca tgtcgagaag
     1801 aaaaaaatac attcaatcaa cagtatcgtt gtttctgcca cgctcattaa aacgattcag
     1861 ccctgtcctc agtgtaaaca ggtgaatcaa catgggctga tcgttaagaa cggaaagaaa
     1921 aaatcgctgg tccttctcaa taagtgtgcg aatcaacgga cttatcttgc tttggcaaag
     1981 caacggtacc tctgtcgtgg ctgtcacacc tattttacgg cgaataccta cattgttgaa
     2041 aaaaactgtt ttatcgccaa acaagtacat tataaaatat tggaggaatt gactgaaaaa
     2101 caagcaatga cgacgatttc taaacattgc ggcgtttctt ggtcaaccgt ttcgcgaaca
     2161 ctcgcttctc tgctccccat gacaaacgtg aaaataaatt ggttgcctcg ctgttcattg
     2221 gtagatgaat ttcgttcatt gaaaaatcaa gtcggtccct attctttttc ttgtatggat
     2281 ggcgatactg ggaaattact ggatattctt ccttcacgta agaagaaaga tctcatttct
     2341 tattttatgc aatttgaacg acgtgcacgt ttaaacgtaa aaatactcgt aacagatatg
     2401 aacgcctctt atgctttgct gataaaagag tgctttcctc atgccaaact cgttgtggat
     2461 cgctttcaca tcgtcaagca cctcattcgc agttttaagg atatttgact atgagtcatg
     2521 aaaagcttcg accgaaacga tccaatacaa gccaaacact atcgacaagt gaaggcttta
     2581 tctcgacttc tgatcaaacg acaggacacg cttgtttatg ataaatggac aaaatggcgt
     2641 aattttggtt gggcctattt aaccgaaagt gaagtcgtag aacgtctact ttctacttct
     2701 gatgaactac gaatagctta tgcttactat caagaaattc ttcaagcttt ttatgacaaa
     2761 gaagcggaca tcttctttca gcttgtaaaa acaatgccca agtcggttcc aagagaactg
     2821 catcatataa aaaaagcgtt tataaactat gaatccggca ttcgtttagc cttagagttg
     2881 ccttactcaa atggcaaaat agaaaatctg catacccaca tcaaagcatt gaaacgaata
     2941 gcttacgggt taggatgaga accagaattt tcttaatcaa tcatctgatc acttattaaa
     3001 cataaaaaaa catctaaagc agaaaatcaa ttctacctta gatgttctta tatcactcta
     3061 tcagtcctat ttgacaaaga gccagaaatg tttgccgact atacactagc tttgttgtgc
     3121 aaaatgctga gtatcaattt ccacagggat tgctagaata tctttcagct gaatgtataa
     3181 ggcatcgcta attttctctt cccgatcgtt caaccgatcc actaaaattt ctttgccctc
     3241 cagcgtttga aaaacatacc gttgaatgtt gcctaaaata aatgattcct tccatagaac
     3301 cggaatctta accccctgag gaaccggttc aaattgaatc agctccggtc ttaaataata
     3361 aatgcagcga ttgcccttag cggaaaactc gtgggtatac tgattcagct cataccattc
     3421 gaaacgatta tagttgccga taaattccgc aacaaatcgg ctctgaggat tctgatacaa
     3481 ttccttcgag gtggaaacct gagccacccg cccagcttcc atcacaaaaa tccgatcact
     3541 gattcgcatc gcctcttctt ggtcatgagt cacaaaaatc atcgtcatcc ctagcgattg
     3601 ctggatagcg cgcaaatccc gctgcagctg ttttctaatt cgggcatcca aagcacttaa
     3661 tggctcatcc agcagcagga ctttcggttc agtgaccaat gagcgagcca gtgccacccg
     3721 ttgtcgttga ccgccagaaa gaaactggac atttttatct gcctgctccg tcatatccac
     3781 caatgatagc atctcaaaaa ccttttcctg aatcacctca ggactcagct tctttacctt
     3841 caaaccaaag gctacatttt caaaaacagt catagttgga aacaatgcat actgttgaaa
     3901 aacaaagccg atattgcgat ccttcgtagg cttatcggca atattctcat tgtccagcac
     3961 aatctcaccg ctctttggtt gttccaatcc cgtgatacag cgcagcaacg tagactttcc
     4021 gcacccgctg ggacctagta aagtaaccag ctcacccttt tcaatctgaa tttcatcaat
     4081 agctaaaatc tgtttttcat caaaaaacag attgagatca tgtatttcta aaaacatttt
     4141 ccgtcacaac tcctttaagg gcttcttttt cgactgcttc tcttccctcg tctgcttaag
     4201 ggacagctgt tgcttttgtc gataagtcag cataaaggta gttaaagcaa ttcccaccat
     4261 aaacagcaaa tacaaaacca tcacagcgct agccaaatgg ccgttttcgt tcatccgccg
     4321 cagcatatag atgcgtaccg tctgaaatcg accgccaatc aacaggttcg tcagcatgta
     4381 ctccccaaaa accgaagaaa aaatcatcag agaaactagg gtcacaccga tacggatgtt
     4441 tggaaacagc acctggataa taatcgtaga catcggggcg cccaaggttt ctgctgcatc
     4501 cactaattcc ttcagattaa ttaagcgcat ctggttgtta agactaagat agacaatcgg
     4561 caacgccgta ataaacaatg cacctaccaa cactacaaac attgggatac cagtttcagc
     4621 atacgttcga agcaaagccg ttaccaaaat cactcctggt aaagcatacg gcagcaggag
     4681 cagcttttcc agccaacgat tcaaacgagg gaaatacaaa tgaatccaaa taatggtggg
     4741 aatcatcaac aacagaatcg tcaccaagac cacacctgct aataaaagtg agcgacccac
     4801 cgcagcaata aattccctgt ccgtcagcag ctgttggaac cattgcaagg taaaatcact
     4861 aggtaaaagc gattttgacc atttagtgga gctggcatac aaaatcgttc caataatcgg
     4921 cagcaagtaa tacaggacaa agcagaacaa aatcggtttt attaactttt tcacgtctgc
     4981 accaccttgc ctttcttttg aataatcaac attgccgtta ccagcaggac acctaaagcg
     5041 actgccaacg cactgcctaa attaggcttg gcgaacacat ctcccgacac taaagccgca
     5101 attcgaatcg ttaaaagatt tacattactg ctggtcaggg catacgctgt ttcataggtc
     5161 cccatgccat tagcaaataa gatgacaaag gtgctggcaa tcgatggtcg caaaaaaggc
     5221 aaagccacct tgaaccaagc atacgctgat gaagcgccca gcaattgtgc ggactccagc
     5281 cattcctttt tcacttcctt catgctggga tacaaaaaca agacccctaa cggaatttgg
     5341 aaataggtat acgtgatggt caatccccac caagaataca aattgaagtc cgctagaaat
     5401 ggcaaaatca ttttcagcac acctacattc cccaacaaaa taataaaaga gaatgccagc
     5461 ggaatcccag caaaattagc tgccaaattg gaaacaaagg tgattttctc ctgcaattta
     5521 gtcgatagat tcattaagca taaacataaa ataaacgtcc cgattaaccc aatcacactg
     5581 gagctgacag caatcagtac gctgttaaca aaggcctgtg tgtaatattt gttgctgaaa
     5641 atatcactgt aattggccaa tgtccactgg ttagttaaat cactgtgcaa gctgcacaga
     5701 accataaaaa gcagcggcag cagtaaaaat gcacagaaga taaaggaggc aggaaccaga
     5761 accaagcgtt ttttcatctc tttcatcccc tcatcaattc ttgaatgtcc tgcattttct
     5821 ctgacggtgc aattcctaat aaccagcaaa acaatggagc cgccagcagc tgtggtacag
     5881 tttgttcctt cagcttttgg cgtttatttg aaatcaaata aaaaggaact tcacgatgtg
     5941 cagccaagct gccgccatgc agtccgtgcg catccatccc gtgatccgca gtcaccacga
     6001 cttgataacc tttttgcagc cattcaggaa ggtaaatagc caataatctg tccgcctgat
     6061 taaccgctgc tgcatattca tgagaatcgg cggtaaattt gtgaccgaca tcatcaatat
     6121 tcatcgaatg tacgaccaaa aagtctggct gatactgatt cactaaataa tgggcatccg
     6181 caaataaatg cgaatcaggg taagtgtcct cactataaaa gatgccatgc tgaatcagtc
     6241 gctcctcctg atgctggatg cgatcaacca aacgattata gggggcttga ttgtacaact
     6301 cgctgtacca gtggtaagcg gcggctcctg tttttccgcc agcttcctta actaattcaa
     6361 acagcgacca ctctttggat cgctgagcat ggtaattact atagatgcca ttttcatagg
     6421 ctggaacgcc ggtcatcagt acttcgtata acggacgtga attgctgggc agctcacttt
     6481 ttaccttcaa taagcttgct tgctggtgct caactaaatg attcatgaac ccaagctggg
     6541 cgacggctgt gtcgtagcga cagccgtcta gtataactac aatcagtttg tcaagtctac
     6601 tttgcatagg tcagtacctc cgcctgccaa agctctggaa gctctacaga ggtcttgttc
     6661 caagcttcat tgtcttcaac tggcaccgcc gcttcataag catcctccgg cagtaatttt
     6721 tcagctactt ctttaggcag ctctaccttg cgaataggtc gcgcatagcc ctttgccaaa
     6781 ttgatttgcc cttcatcaga taaaatatat tcccgtgcta gctttgccgc attgggatga
     6841 ggggcatttt tattgattaa ggttgtatac ccgctaatga tggagccgtc tgttggcaca
     6901 gttacctcaa aacgctcgga gtcaatttgg tcacgataat ttagtccatt gaagtcccaa
     6961 acaactgcca catcgatttc acctttttca agatttgcta cgctggcatc aacggtggac
     7021 aaacggcctt tttctgccat ttttttgtag taattcagac ctggctgaat atccttttcg
     7081 tcacctccgt tggcaatcgc cgcagccaat acagcaaatt gtgcttggtt ggcaatcgtc
     7141 acatccccca cgctaacttg atagtcaccc tcttgcagct ccttccagct agtcggtgct
     7201 tgcttcacat tctctttatc ggtgataaag gaaatggttc ccgtatagct taacaaccaa
     7261 tccccatcat catcctttgc ccaatccgga atttcctccc aatagttggt cttataaggt
     7321 aaggttaact ctttgctttc agccaacgga ccgaagctga taccgacatc accaatatcg
     7381 gcggttccat tttttccttc tgattcaaac ttagccaatt cttctgcact ggacatatcc
     7441 gtatccacgt gacttaagcc gtattctttt tcaaggtcgt tccaagtgcc tacccaattt
     7501 gcccaagtat ccggcattcc caagctggca atctctcctt cttccttggc tttttctata
     7561 atcgtatcta aagagtcgtc tgccgcagcc gattgcttcg ttgcttcacc tgagacgcag
     7621 cctgtcagca ggatacttac tgttgctgca caaaataaac cgatagtcat gttacgaatt
     7681 ttttcattca ttttcattca ttcttctccc ctttcgataa ttccattcta acaaagcttt
     7741 gtaatgagag aatcagtttc ttgaaaaggt ttcatgaaca atgtaaaggt tgtatatggt
     7801 ttgtaaattt cgacgaattc ccgttaacct tctaaagctt ttgcggtatg atagtagaaa
     7861 aaaggaggct ccctatgcag tattatgatc agcatctcca cacgtatttt tcctttgatt
     7921 cagaggaaaa atttgaaaac tatttagcct atcagccgga gtttttcgtt tctacggatc
     7981 actttgattt aaacaatcca tctaccggaa aagatgacat tccggattat ccagcctata
     8041 taaacaaact ggactcttta aaagaaacct attccaccaa atttctgcga ggtatcgaga
     8101 ttggcgttgt tccaggacaa gaagagcgga tcgtggatta cctcgctcag catccttatg
     8161 atttaaagct ggtaagtatt catcaaaatg gccgctttga ttatatggac gatgtggttt
     8221 taaccaaaga tcccattcaa actaccaagg aatattttga tcagatgttg caggtcttga
     8281 cggattttcc agaaggagat attttgactc actttgatta tggattacgc cgcttcacct
     8341 tcacagcgga agaattcgcg gagcattttg aggctcagct gctaacgatt ttcaaaaagg
     8401 ttgttgaacg agaattggcg atggagttaa atgccaagag cttcttgaaa tacggcaacg
     8461 aagaccttta ccgttatgcc gttcctttgt atcaaagtgt cggcggaaag ctctttacct
     8521 taggctcgga tgctcatgtt gccgaagatt atcagctggg ctttaaaaaa atgagtcagc
     8581 tgttggcaga ctgtcaggta gaaaagttgc tagtcattca aggaaaagaa cgccgcctga
     8641 cgccgctgcc gattttgtga ttcctatata atagaagaaa gtggtgaaaa aaatgagcaa
     8701 cctgtgggac tacaatcaag aggcacccat ccattattta attgcgcgac attgggatgc
     8761 gttgaaaatt gaggcggttt gccgatcctt gttagcagcc gttccaaaac aacaactgga
     8821 aaattttttg gtggctgaca gcttgcagcg ggaaaaagtt caggcttatt ttgcagcctt
     8881 taaagatcag cctttggaat atttgcatgc gcagtttcat cttttttatc aagtagccgc
     8941 cccagatgac tacaacgatt tacgaggaca gctgcaattg acctttcaag cggatgaaac
     9001 cgcctatacc gttttattgg gaatggctcg ccttggtgat caggcgaagg tcgagtggcg
     9061 gattttcgat atttgatggc aggatcaagg cgtttcagtt gcaaaccttc ctctctttca
     9121 attagaaaaa ccgcagcagg aagaagtttg tgtgctggtc accgatgctg gtgaaattcg
     9181 tctccgctta tttcctgacc aagctcccct ggctgtagcc aactggcgct ccctagctga
     9241 gcaaggcgtt tatgacaaca cacctttcgg ctcgtgtcat taaggaattt gttattcaag
     9301 gtggcgcttt ggatggttct ggtgatgaag ctgtttccag ctatgagggc tttttcgctg
     9361 atgaagtgca tcgcggactt tatcatttta atggcgcctt ggccttaggg gaccacggcc
     9421 cccacaccaa cggcaaccag ttttttattg tgcaaaacac aaaggcgcag gctgacctgc
     9481 taatgtaaag caagcatact tggagcttgg aggactaccg gagctggatg gtcgttatac
     9541 tgtttttgga caggtatacc aagggttaga aatcgtagca gcgatcgcag ctcagccgac
     9601 aaacagtgaa gatgtaccga ttgagccagt gttcattcga caaattaact tcgaaaaaac
     9661 atcgtaaaaa gacactcact gacttcagca aaagctctat tcttgagctg gctgcagcta
     9721 gtgagtgtct ttcatttatc cataagataa agcttggatt caataaataa ataaatgttt
     9781 tggttaaagc tgtgaccagc aaatgaggaa ccgtgcgagc cagttgatct gccaatgcaa
     9841 acatttcttt aggtgaaggc gctggcgtgt ctgccaacgg atactccagt cccaatgctt
     9901 ggtaaccgtg cataccatag cagtggtacg gtagtaattc taacggtacg aaccgatcaa
     9961 gatctgccac aaaacaagca atcgtcagca gctcggtcag gttcatattg atacctggaa
    10021 ccacaggaac tcgaattcgc acttcgcagt taggatactc aattaaaaag cgacgaatat
    10081 tcttcaaaat ttgttggtta tctaagccgg tccattgttt gtggagcttg ctggtaaaca
    10141 tttttacatc cacaaacaat gtatccacat agggagctac cttttggatt tcattataag
    10201 caccacaaaa gctggtttcc agtacggtat tgattccaat gtatttcgat ttttgcaaaa
    10261 tttctttagc aaaatccgcc tgaaccagtg cctcgcctcc gctaatggtc actccgccat
    10321 ccgagtgaaa gtaaaaaaca gcatccttct caatctcatc cattacctct tcagcggtca
    10381 tggtctggcc aaatcctttt tcaatctgct tgctttgtga ttcgggaatc gaacaccagg
    10441 cacagcgtaa tgggcagccc tttaaataaa caacggtccg taaaccagca ccatcatgta
    10501 gagaaccctg ttctattctt aatactgttc cttccatata gatttttgaa ttacacacta
    10561 gtttaaacta aaacacacaa ggaaaaactt accatttaat cataaaatta gctcatataa
    10621 atatactagg atgtaaaata gactggtcaa aatctggtc
//
LOCUS       DAHEYX010000093        10485 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHEYX010000093 DAHEYX010000000
VERSION     DAHEYX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.094"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            10..177
                     /locus_tag="MB219_001608"
     CDS             10..177
                     /locus_tag="MB219_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCC8427325.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(239..2050)
                     /locus_tag="MB219_001609"
     CDS             complement(239..2050)
                     /locus_tag="MB219_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCC8427326.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2047..2412)
                     /locus_tag="MB219_001610"
     CDS             complement(2047..2412)
                     /locus_tag="MB219_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCC8427327.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2575..2970)
                     /locus_tag="MB219_001611"
     CDS             complement(2575..2970)
                     /locus_tag="MB219_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCC8427328.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2988..3950)
                     /locus_tag="MB219_001612"
     CDS             complement(2988..3950)
                     /locus_tag="MB219_001612"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCC8427329.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3950..4162)
                     /locus_tag="MB219_001613"
     CDS             complement(3950..4162)
                     /locus_tag="MB219_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCC8427330.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4183..4881)
                     /locus_tag="MB219_001614"
     CDS             complement(4183..4881)
                     /locus_tag="MB219_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCC8427331.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4901..5443)
                     /locus_tag="MB219_001615"
     CDS             complement(4901..5443)
                     /locus_tag="MB219_001615"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCC8427332.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5575..6579)
                     /locus_tag="MB219_001616"
     CDS             complement(5575..6579)
                     /locus_tag="MB219_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCC8427333.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6576..7565)
                     /locus_tag="MB219_001617"
     CDS             complement(6576..7565)
                     /locus_tag="MB219_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCC8427334.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7562..8368)
                     /locus_tag="MB219_001618"
     CDS             complement(7562..8368)
                     /locus_tag="MB219_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427335.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8534..9490
                     /locus_tag="MB219_001619"
     CDS             8534..9490
                     /locus_tag="MB219_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8427336.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9567..10085)
                     /locus_tag="MB219_001620"
     CDS             complement(9567..10085)
                     /locus_tag="MB219_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCC8427337.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10173..10322)
                     /gene="rpmG"
                     /locus_tag="MB219_001621"
     CDS             complement(10173..10322)
                     /gene="rpmG"
                     /locus_tag="MB219_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCC8427338.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3256 a         2293 c         1791 g         3145 t
ORIGIN      
        1 gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc aacagccgct
       61 gccgttgctg gtttggttgc ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa
      121 gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa
      181 ttatttaaaa atacgcatga agactttcta aaaaacagat aagtcatcat gcgtattttt
      241 atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc catggatctg
      301 tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt attccatagc
      361 aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta cctcgctcaa
      421 catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa gggcgaacaa
      481 gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag cttgtttgtg
      541 gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga gaaataacat
      601 agcggttgtg cttttgctga atatcaaatg gcggttcgat caaatgaaaa atacccgcat
      661 ctatcagtgc tgctaattct ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat
      721 ttagatgagt aaaccatccc catagtattt ctttgtattc cttagcagaa aaaacgtgcc
      781 attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa
      841 atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata aatattcggc
      901 tagcttctga aaagggctca ttctgatagg caagaggagt ttctaaatac gaccaatccc
      961 atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag agggagagaa
     1021 aatcatgctc aaaatttttc tttggaatcg gcaagtgata ttcttcgatg attttcttgt
     1081 aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc
     1141 ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat gccattgctc
     1201 cgccttgctt ttgatttctt cctcgagggt aataagggag cccccttctt gatccactgt
     1261 atacaatcgg ttccttgcct gatggatgat agaccagttt tcccttttcg ttctcgaagc
     1321 gccctcctct tgatactgta aacaatccga cataatcaaa aaaacttaag cctagacctc
     1381 ttaaaaagac attttcttta ggcggtatct tcgaaagatc tacatctgca ggattagaag
     1441 gaagctgata cagtaaaaca tttttctttg cgtagtctac ccacactctt tcttcttctg
     1501 tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa tagctttgct
     1561 gatctgtctg taaaagataa ccatcttttc ttttgcttac tttttttact aaagaataaa
     1621 caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag cgttgataga
     1681 gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt tcttctataa
     1741 ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt cctttactcc
     1801 attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt tcatctgtaa
     1861 aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga ttcaaccgcc
     1921 aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct
     1981 gattattatg gagaatcctt tctgctacac ttaatcctct aggtccggca ccaatgatcc
     2041 ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag catggttctg
     2101 atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca taagttgact
     2161 gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct ggtatttctt
     2221 tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg
     2281 tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc ctcgtggcca
     2341 gaatctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt aaacacgttt
     2401 taatttgatc atagttgttt ccttctttct accttttcgc ttagcttata tatgtagata
     2461 atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc atgtgatctt
     2521 ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc tgttttatac
     2581 ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta aataaatctc
     2641 ttgataacct gctttacgta atttatttgc tgtacggatg ctcaaacttt tcttttgatc
     2701 gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca aaacagtgta
     2761 agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt cgcgaacatc
     2821 gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag tcgttgctga
     2881 acgtttggcc attactcgta gatagacttc ccaaaggatg attgcaagca caatcaatag
     2941 caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta agcaaatttc
     3001 aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc taatttgatt
     3061 tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc tacacggcta
     3121 cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca cgatacttga cggatttaat
     3181 gtattcccca agtttccgca agctaaacct aagtagaagc atacgcgatc aacaaccatc
     3241 aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt tacttcttct
     3301 ccgttgtcta gcattgcttt cagttttgaa tcccctgcgt attcttctgc caagaaacga
     3361 cctaggcgta ccacacctgt agcgctagat acagtttcta aacatccgcg tttaccgcaa
     3421 gtacattcga acccttcagg atccacagtg atatgtccga tttctcctcc agctccgctc
     3481 acgccgtgaa tcaaatttcc gccagcaacg atacctccgc caacacctgt tcctagagta
     3541 acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc taaagcagca
     3601 acattcgcgt cattatcgat agcgaatttg attcctgttc ctgcctcgat cagttttttc
     3661 acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc agcttcgctg
     3721 tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt catattatat
     3781 aaattcaaac gatgattgat tgattcaatg atttcaggaa cgatatgctt accatcatcc
     3841 aaaatattgg tatcgatgct ccatttttgc tgaatctctc cttctggtgt taaaattgca
     3901 aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat tacaaatttc
     3961 ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac gctgtcatgt
     4021 atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata gtcattaatt
     4081 caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg aacagctgct
     4141 gtacatcata taagctctcc atctttaagc acttccttca tattatacaa gtaaaccata
     4201 tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta gtgtagaaag
     4261 aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa cattccctaa
     4321 cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg tatccagaag
     4381 accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct gctggatcgc
     4441 tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac cgaatatcga
     4501 agtacttgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc
     4561 cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga ttgcctcgat
     4621 ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca gtccaaaatg
     4681 gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat aaggaccata
     4741 cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga ggaagaccgc
     4801 tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg cacgcaattt
     4861 tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata aacaattgtt
     4921 ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca cgaaaaacaa
     4981 ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca ccgccaaaac
     5041 cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga tcgatcatct
     5101 ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa cgtacaagaa
     5161 cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa
     5221 ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt
     5281 cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg gcgaatagct
     5341 gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga tttttcagct
     5401 tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca cccttccttc
     5461 tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat
     5521 aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaatg aatttcaact
     5581 gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg caccaaaaag
     5641 ggcaatcaaa attcctgtag gtacttcccc acttagtaga atcactctag caaccatatc
     5701 tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc agttattttc
     5761 tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag ataaactacc
     5821 tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga ttttttgact
     5881 tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata gcaatacaat
     5941 cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa gcaatgccca
     6001 aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca caaatccata
     6061 attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc cggcattaac
     6121 ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat cataggccgc
     6181 aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta gcaatagaaa
     6241 cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc ctgctccggc
     6301 attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt gcaagatata
     6361 accagaaacg ccaagacaag ctcctgctaa taaactgatc agtaatcgag gaactcgaaa
     6421 ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg caaaaagctg
     6481 atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga tgacaagtac
     6541 aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct ccttacttgt
     6601 atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg
     6661 ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc aactaaaaaa
     6721 gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac tggcggaacc
     6781 aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc taacactgca
     6841 actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt
     6901 gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac caaaacagca
     6961 agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc agctccgcct
     7021 acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata
     7081 gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg attcatgccc
     7141 attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga acctgccaat
     7201 gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact aagggccatt
     7261 gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg atttcttgtc
     7321 actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc
     7381 acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct atcaaaatca
     7441 aacaacgcct gccaaaaatc tgcttttccc acagtcgctg cgccatttga aagagcaaca
     7501 aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt cttcatagtc
     7561 ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa actggtttat
     7621 ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc atacactctt
     7681 ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc attgctaata
     7741 aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag atcgttgttt
     7801 gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt gccggatcta
     7861 aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc gccatagcga
     7921 tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata cgaaatccag
     7981 ctagacctgt agcatccaac gcccactcaa ttgcttcatg atcttgcgtg tccatgccac
     8041 tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact gtcatacctg
     8101 tagggtgttc actagtttgt gctaataatg caattttttt tgcaagttct tttgaactgt
     8161 atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt agtatacggg
     8221 taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa ctagtgattt
     8281 tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg tagccggctg
     8341 caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa atcattctca
     8401 attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag ccaaatgaag
     8461 cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc tcaattatgt
     8521 taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca tattgctgct
     8581 ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta caactgccaa
     8641 ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac ataaagtaga
     8701 gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc tgattgcact
     8761 aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag attatctgca
     8821 agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga aagtgttaag
     8881 ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg ggaaatacga
     8941 acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg tcacatggga
     9001 agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat
     9061 ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa
     9121 caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca gcaaaaatcg
     9181 gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc tagtggaaga
     9241 ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt
     9301 agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc
     9361 aagctggaag aatctaaaag cagtgacaga caatcatgta ctgcaattcg gacctgaatc
     9421 tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa
     9481 actaaattga ttttaactaa aaaaagcatc ttcacgatta gtcgtgagat gctttttttc
     9541 ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct attttttact
     9601 ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat cccctcatac
     9661 tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc gccttcaaac
     9721 gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc
     9781 cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga caaaagttca
     9841 tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac
     9901 acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt ctttcttccc
     9961 atcactaaaa ttcgatccat tgtgtgttcg cgaaagaaac gcatatcatt tggcaatcgc
    10021 catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca catggaaata
    10081 aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt ctcaattctt
    10141 tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg caaagtaact
    10201 ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt acgtttgttt
    10261 ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat gtttacgcgc
    10321 atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac ttgattatct
    10381 taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta aaactacttg
    10441 acgataaata caacggtttt acttctcaaa caaaatcttt ttctt
//
LOCUS       DAHEYX010000094        10443 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHEYX010000094 DAHEYX010000000
VERSION     DAHEYX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.095"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(111..596)
                     /locus_tag="MB219_001622"
     CDS             complement(111..596)
                     /locus_tag="MB219_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCC8427339.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    686..1031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1076..1717
                     /locus_tag="MB219_001623"
     CDS             1076..1717
                     /locus_tag="MB219_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCC8427340.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1736..2371
                     /locus_tag="MB219_001624"
     CDS             1736..2371
                     /locus_tag="MB219_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCC8427341.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2389..3204
                     /locus_tag="MB219_001625"
     CDS             2389..3204
                     /locus_tag="MB219_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8427342.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3523..4959
                     /locus_tag="MB219_001626"
     CDS             3523..4959
                     /locus_tag="MB219_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCC8427343.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5148..7595
                     /locus_tag="MB219_001627"
     CDS             5148..7595
                     /locus_tag="MB219_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCC8427344.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7825..8892
                     /gene="rlmN"
                     /locus_tag="MB219_001628"
     CDS             7825..8892
                     /gene="rlmN"
                     /locus_tag="MB219_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCC8427345.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9085..10188)
                     /locus_tag="MB219_001629"
     CDS             complement(9085..10188)
                     /locus_tag="MB219_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCC8427346.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3442 a         1792 c         2271 g         2938 t
ORIGIN      
        1 gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct
       61 aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt
      121 tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata
      181 atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca
      241 gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact
      301 gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc
      361 ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg
      421 acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca
      481 ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa
      541 gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg
      601 catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc
      661 atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg
      721 tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg
      781 agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt
      841 tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc
      901 acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca
      961 acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat
     1021 acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga
     1081 gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt
     1141 tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt
     1201 gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc
     1261 gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga
     1321 tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat
     1381 ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca
     1441 attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt
     1501 accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt
     1561 aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact
     1621 gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat
     1681 gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat
     1741 tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct
     1801 aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac
     1861 gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg
     1921 tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca
     1981 agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat
     2041 ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt
     2101 agggattagt gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg
     2161 ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac
     2221 aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga
     2281 agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga
     2341 tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc
     2401 agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa
     2461 acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac
     2521 gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt
     2581 gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac
     2641 tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat
     2701 tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat
     2761 caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag
     2821 agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta
     2881 ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg
     2941 atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat
     3001 tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt
     3061 gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa
     3121 gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc
     3181 gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc
     3241 cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca
     3301 ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt
     3361 tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga
     3421 ctttttcaca acctccttta catattttta ttttgttgtg gaaggggttt cgtgttattc
     3481 taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt
     3541 acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga
     3601 gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga
     3661 aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa
     3721 gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca
     3781 cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga
     3841 ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat
     3901 cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat
     3961 ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt
     4021 agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca
     4081 ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca
     4141 tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg
     4201 tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca
     4261 gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct
     4321 gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa
     4381 ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat
     4441 gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt
     4501 tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa
     4561 cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc
     4621 acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag
     4681 ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc
     4741 aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct
     4801 tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt
     4861 gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg
     4921 taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa
     4981 ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac
     5041 tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc
     5101 cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga
     5161 aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg
     5221 aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt
     5281 taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata
     5341 cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata
     5401 cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta
     5461 tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca
     5521 tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg
     5581 ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc
     5641 ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc
     5701 tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat
     5761 tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac
     5821 agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag
     5881 ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat
     5941 ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc
     6001 aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca
     6061 atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg
     6121 agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt
     6181 ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat
     6241 tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac
     6301 catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga
     6361 acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga
     6421 ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct
     6481 ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa gtacctttag
     6541 gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg
     6601 tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat
     6661 taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat
     6721 tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag
     6781 cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg
     6841 caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa
     6901 acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt
     6961 tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc
     7021 gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag
     7081 gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt
     7141 ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat
     7201 tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg
     7261 aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa
     7321 tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt
     7381 ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa
     7441 gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg
     7501 gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa
     7561 catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa
     7621 acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc
     7681 atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat
     7741 tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac
     7801 tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa
     7861 gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg
     7921 gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa
     7981 ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa
     8041 gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag
     8101 actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt
     8161 aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct
     8221 ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc
     8281 gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat
     8341 gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac
     8401 attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg
     8461 caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg
     8521 cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag
     8581 aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca
     8641 gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac
     8701 ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat
     8761 gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa
     8821 catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca
     8881 gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt
     8941 aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct
     9001 ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg
     9061 cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg
     9121 tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc
     9181 ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc
     9241 ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa
     9301 cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc
     9361 ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta
     9421 atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc
     9481 ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc
     9541 actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat
     9601 ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc
     9661 gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc
     9721 aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc
     9781 cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc
     9841 aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc
     9901 gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg
     9961 gatcaactgt tcaatatttc cataagttga attacccccg taccagagac tggcgacaac
    10021 ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt
    10081 ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata
    10141 cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt
    10201 ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga
    10261 tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga
    10321 aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg
    10381 aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa
    10441 att
//
LOCUS       DAHEYX010000095        10378 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHEYX010000095 DAHEYX010000000
VERSION     DAHEYX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.096"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..715)
                     /locus_tag="MB219_001630"
     CDS             complement(<1..715)
                     /locus_tag="MB219_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8427347.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRK"
     gene            898..2022
                     /locus_tag="MB219_001631"
     CDS             898..2022
                     /locus_tag="MB219_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCC8427348.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2249..3166
                     /locus_tag="MB219_001632"
     CDS             2249..3166
                     /locus_tag="MB219_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8427349.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3159..3992
                     /locus_tag="MB219_001633"
     CDS             3159..3992
                     /locus_tag="MB219_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8427350.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4075..5193
                     /locus_tag="MB219_001634"
     CDS             4075..5193
                     /locus_tag="MB219_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCC8427351.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5187..7085
                     /locus_tag="MB219_001635"
     CDS             5187..7085
                     /locus_tag="MB219_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCC8427352.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            7079..8302
                     /locus_tag="MB219_001636"
     CDS             7079..8302
                     /locus_tag="MB219_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCC8427353.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8388..10322)
                     /locus_tag="MB219_001637"
     CDS             complement(8388..10322)
                     /locus_tag="MB219_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCC8427354.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
BASE COUNT         3378 a         1669 c         2058 g         3273 t
ORIGIN      
        1 cttttctaaa atgttttttt aagctggctg gactgataaa aaaaatttgg gctaattgtt
       61 caagcaaaaa attttctgaa agatgttctt ctaaatatcg tatgattgga tcaactaatt
      121 catctgaata aagaatatgc ttttttttag gaatgacttt ctcatttgaa ggtattgtca
      181 ataaagcaag ttcctgttga taatgccttg gtaaggaaga caattgaatt ttttgcagac
      241 aataagtaac taataaggaa agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag
      301 aattgatttc ctgatcatag gaaatagctg gcttactatg ttttagataa tcaattttga
      361 actgttcatt tttcagaaaa gtaatgatag atgttatttc ttgatctata gaaaaagatt
      421 gtcccgtaat atgtgctaat aattgctcat caaaataaaa ctggataatg ataaaagttt
      481 gtgtttcctt ttgagtagaa aagatttgga cttcttgctg tttatcaatg aataaaaggg
      541 aaggattatc tagttctata gatttatgtg caaattcagc atataaatta gcgctttcat
      601 ttattagtag tcgaaaatag ggagcagcag ataaatgaag atgggtatct atggaatagg
      661 ttagtgtttt tttgacaaga ttggaggatt ggttgtttgt taacaaaaga accactttcc
      721 tttctctttt tatataaaaa gctttctatt tataattaat gactattata acaaaaatga
      781 aaagccaaaa cgacaagtaa aatatttttt tatataaata tttgtgttac taattccgtt
      841 aatacacata agtgaaagcg ttttatataa gaacttgaaa agaaagaagg gatagacatg
      901 aaaaaaagat acaaattggt gatagctttg ctaaacatta ccctattatt cagcttaagt
      961 gcatgtggaa aaactaacga agaagtaaca gcaaacatcg cagatccaat gataaaaaaa
     1021 gccactacat tagaagtata tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct
     1081 gcaaaaatct ataaccgcaa aactggagca aatattgact taaaaattgt gaatgtagct
     1141 tcgggaacag caacagtaca aatgatcacc ccaaaattgg taacacacga agatatgccg
     1201 gatattatct ccatggcaga cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct
     1261 ttttatagtg ctgatgaata tggattttat gacaaatatg gcgatcagtt ttatgatcaa
     1321 aaattgaata ttttgcgtca acaaacgagt aatgaaacga ttattccttg ggctagtgat
     1381 tttaccccag cactttgtta ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt
     1441 gaagaaatca gttcttggga agaatatatc caggtggcaa aagcattaaa agaaaaaact
     1501 ggtagttttg gaattgcctt gccagaatct ggggaccaag aattgtttat caacatgcta
     1561 gcccagcaga atcaatcttt gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa
     1621 gcgaaacatg ctgcaaagtt aatcaaagaa atgatcgact cagatattgt acatttctac
     1681 ggtgcacaag atgctgagaa agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg
     1741 tatgctacga atatgtcttt gaattttcct gatgcatctg acaaatggcg catggctcca
     1801 ctgattcctt tttcaaaaga aaatccagga aaaggtgcag tatcaggagg atcaagtttt
     1861 tatgtcccta aagatgccaa aaatccacaa gtggcacaac aatttcttac atttatgctg
     1921 acagatgaag aatgcttagc caacgcactg gagctgggcg tggctacatc taactcattg
     1981 gcctatcaaa cagaagcagc tgagaaaaag tttgactact aaggaggaca aaaatactat
     2041 gaactattga ataaaatcaa tgaaaataca tcaaatactg ttttaccagc cacttattca
     2101 gatgctacgt cttatttgac taccgctgca tataactatt ggcaaacagg tgattttgaa
     2161 aattcatatc taaaagaagc agaaaatttt gctcataagt acggcgtaaa attaactggt
     2221 gattaaaaaa tattaggagg acttaacgat gatgcaatct tcgttaccaa gcaagacaat
     2281 aaagaacagc aagaaaaaag caacagatac gtctcataaa aaaagtgctt atggattggt
     2341 cacacctttt gttattttct ttattgtgac gattttgatt cccatcatta tctcgtttgc
     2401 ttttagtttt atgaaaattg gctactcatt tgaatttgtt ggatttgaca attatctagc
     2461 tatcttccga gatccattgt ttattaaatc ttacacgaat gttttgatta tcatggcagg
     2521 atcaattcca ataacgatgc tattagctat ttactttgct gttttattga attcagcaca
     2581 gttaaaagga aaaggatttt ttagaactgt ctattatatt ccaaccatta catcaacggt
     2641 ggctgtagct tccgtattca tgactttttt caatccgact gggttattca atggattttt
     2701 gaatgcgcta ggaattaacg gtgtacagtg gttaacaaat cctttttgga tacgaatatc
     2761 aatgatcatc acgatgattt ggcttagtgt aggatataac actattttat ttttagctgg
     2821 tcttcaaggg atttctaaag agatatacga aagtgctgaa atagatggag catcgaaatt
     2881 tcggcaattc tttttattaa cgatccccat gctaaagcca atcattttaa tggccacagt
     2941 tttagccact atcaatggct tgggtaattt caatatccca aatatattct ttgggacttc
     3001 caatggacca gaaaattcag ctttggtcgt aggagtcaat ttatataaaa caagttttga
     3061 aatggttgat tttggaaaag cctctgctat tgcctggacg atggtgtttg tatctgttat
     3121 attatcggct attcaattta aattcggagg tgataaaaat gactaaacaa aagatacaaa
     3181 gcagaatttt tacgtacgtc attattgtgt ttggtgcctt aatatttttg atgccatatt
     3241 tatatatgat tgtagcttca acgcaaagta atcaagaaat tttaagtggc ggaatcaatt
     3301 tcaagtttgg ggatatgcta ttaaaaaact ggcaagccat catggcgaga tataattatc
     3361 ccttgattat ttggaattcg attgttattg ctgtaatcag tacagtttta gctacagcca
     3421 gttctacaat ggcgggttat gcattagcaa agtatcgttt tatggggaat aaactgttgt
     3481 tcaatacagt tatgctatct aggatggtgc ctttttttac cacattgatt cctttatttt
     3541 acattatgag tcgtttagga ttatcgaata cttatgcagg tgtgatcatc ccttcgattg
     3601 ccagtacaac ttcagtgttt atgatgcgac aatatagcag tcaatttcct acagcactga
     3661 tggaggctgc cagaatcgat ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac
     3721 cgattttatc acctacgatt attactagtg gtttacttat ctttatggga agttggaatc
     3781 aatatctatt tccattagtc atgctttcag ataaagataa atatacggtt cccttaatta
     3841 tcaaaaatat gacggtttct agtacaggtg agccaattaa ttatggcgca attatgctaa
     3901 ttttagcaac ttcagtgatc ccaatggtct tgatttatgt gtgggcgcag tctaaattta
     3961 aggagaatga cattggagct gcaaataagt aaaaaaaata acgaatgtta accatctagt
     4021 tcgttctagg agcaactcca cattcataaa aattaaaaga atggcgggat atccatgaat
     4081 gaacagacga aggaatggtc aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg
     4141 gaattagaca gaatggggaa taaaattcca tatatttctt acaacggaaa atatacagac
     4201 gtaataaagg acagaggaat cgactggtgg accaatggtt tttggagtgg tattttatgg
     4261 cagctctttc acaagacaaa acgagaaaag ttcaaagagg cagcaataca acaagaacaa
     4321 ttattagaat ctgccctgta ccatttttca gacgttcatc atgatgtagg ttttttattc
     4381 ttaccatcgg ctattgcaca ttatcaacag tctcaaaaca aacatagtcg acaactaggg
     4441 ttacatgcag ctacggtact tgctggacgc tataatattt cagggaacta tttagtagca
     4501 tggaatgacc ataaacctgg ttggatgatt attgatagta tgatgaattt accattactg
     4561 tattgggctt cgagggaaac tggtgatccg aggttctcta aaattgctcg gcagcatgca
     4621 gatacaacag ctaagtatct agtgaggaaa gatggatcaa ccgggcatat cgcagaattt
     4681 gatgcagaat cagggaaata tattcggtta cttggcgggc aaggatacaa tgagaattct
     4741 tgttggagca gaggaaatgc atgggcttta tatggattcg cgttaagtta tcgttacaca
     4801 aaaaatccgt actatctagc tatagcagaa aagatagcag taaattttat tcagaaaatc
     4861 gaaaaaactg gttatattcc tctcattgat tttgctgtaa aagattcgaa tattgatacc
     4921 agtgctggag tatgcgctgc atgcggtctt ctggaattag caggatatgt ttcctctgta
     4981 cgatcaaaaa tgtatcatga agctgctatc aatatactcc aaaagacaga aaagcactat
     5041 gcaaattgga atatggatga agatgggatc attggaggcg gagcagaagc ataccataga
     5101 gaactgactt ataatgtgcc actgatctat tctgattatt tttttattga aggcttactt
     5161 cgattagaac agtcggaatt agctatttgg tagtaaaaag aggagaaaat acaatgaaac
     5221 atccagtacc actattaagg accagacagg attactttga cttatttaaa aagataaatc
     5281 aaccactcca gccgttttat cgagcaagtg gcgcacaaat tgattatata catcaagggg
     5341 taggatatgg aaataaaatt gcaggaatgg aaggattctc tcgggtttta tggggagcgg
     5401 ggcccgcaat tgaccaatta gatgaacgat ggttgaaatt aattctatca ggaataaaag
     5461 ctggaacaga tccttcccat cccgattatt ggggagaaat acatgaaaga gaccagcgta
     5521 tggtagaaat gcccgcaatg ttattagcgt tatatcatca tgaacaatta ttatggaaaa
     5581 aattagatac aggagaaaaa agccagatta ttcattggtt ctcgcaaatt tttgaatatg
     5641 aatgtgcaga tggtaattgg caatttttta agataattgt cggaacaatt atccaaaaat
     5701 ttggttatcc agttaaagaa tcagcactga aagaagcatt tgataatata gaaaattgtt
     5761 atcttcaaaa tggctggtat cgagacagtt caagaggtcg ccaagattat tataatcctt
     5821 ttgcatttca ttattatggt ttgatttata gcgtactaga accagaaaag cccattagcc
     5881 agatatataa gaaccgagct attgattttt ctagagacta tattcatttt tttgcggaag
     5941 atggtgcaaa tgtgcctttt ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt
     6001 ggtcagctat gttgtgggcc gatcttttgc cttttaaatt aggtgaaatc aaggggttag
     6061 tcaatcggaa tataagatgg tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga
     6121 atattggtta tacctaccct caattgacat tgactgaacc ctataatagt acgttatctc
     6181 cattatggct gaataagatt tttttattgt tggcgctacc tgaagaccat gagtattgga
     6241 cggctaaaga aatgcctatg cctattcgtg aaggaactaa acttctttca gaagccaatt
     6301 tgctcataca acatgataat ggacatacat tttttttgaa tgcgggacaa ttagggccaa
     6361 atttccatac attgaccaat gaaaaatatt taaaatttgc ttattctagt caatttggtt
     6421 tttcgatacc gcgagcgaat caattgaaag cagaaatggc aatggattca atgctaggga
     6481 ttcagcgagc ggacacatat atcacgacca gttttaagaa tgagagcatc aaaacctatg
     6541 gacagttcat cgttcgaaat aatgtacgag acattgtatg taagaaagag gtacttgcct
     6601 ctacatggga acctacttta caggcaaaaa tcagaacttg gcttattcct tttgaaggct
     6661 ggcagattcg cgtacacaaa gtagaattag atacagaata tgttctatat gagacagggt
     6721 ttgctattga atgttctcct gagaaagaag gaataatcat agaagaagac aaggaaaact
     6781 atagggtgag tgaagctggc ttttcaggta ttatctgttt aagtgacgat actattaaac
     6841 gaaaatccac agctattatg ggtttgccca atacaaactt gatgacatgg gaaaacactt
     6901 ttattcctgg attagagggt acatttggaa aaggaaccca ttggctcgga acaggagtgg
     6961 ttgctcatca agatcgggat tatagtttag aagtatggaa caatcgtccc aaaattgatt
     7021 ttgatgggac aaccgggcta actatccaaa ataaaaacaa taaaaaaaga ctaaaattat
     7081 gctaaaggct ggtggggaaa tgcttgataa agtgatgttt gaagagataa tacatccaga
     7141 aaaatggata aatgaatgtt ataaagaatt aatggaaaaa gaatggaata atgagatcga
     7201 gcgttgtcgc agacacacgg agaggttaat caaacgtatc agtaacagtc agtatcaatt
     7261 cccaagcgag tgtcctataa aaggacgtta tccattaaga gacaatattg gttggacaga
     7321 aggattttgg acaggaatta attggttgtt atttgagaag acgggtgatc ctttatttca
     7381 acaaacagct gaaaaagcag atatagattt tatccacagg attgactgtc aaattatcac
     7441 taatcatcac gatcttggtt ttttgtattc tttatcaacc gtagcagatt ataaaataac
     7501 gggaaatcga aaaatgaagg aagcttcaat tgatgctgca gaaacattac ttcagcgttt
     7561 ttgggagaag gcaggagtta tacaagcttg gggagatcct agtgatcctt cacaacaagg
     7621 tagaatgatc atggattgtt gcatgaatct tccattattg tattgggcga gtgaagaaac
     7681 tggaaatcaa aaatataaaa aagctgctca ggctcatatt caaaatgcag cagattattt
     7741 agtaagagaa gatggttcaa cttatcatac attttatatg gatgtcaaag atggaaaacc
     7801 aagatatgga agtacgcatc aaggttatag tgataattcc tgttgggcta gaggccaagc
     7861 atgggggatt tacggttttg cattaagtta tcgttatttg aaagaacaaa aatattttga
     7921 tttaagcaaa atattagcca attattttat gaatcggttg ccagaagata ccatttgtta
     7981 ttgggatttg atctttgatg attctagcag agaagaaaaa gatacatcta gtatggtgat
     8041 catggtttgt ggattattag aattaataga atcaggtcta ttagacaaaa cagaaaagaa
     8101 cttataccag tctgctgttt ggaaaattat gcgacaattg aaagaaagat atgctacaac
     8161 ggaagaagaa gatggctttt taacacagtc tgtttataat aagcccaaaa ataatggggt
     8221 aaatacacct tgtatttggg gtgattacta ttatttagaa gctttagtga gaatgactag
     8281 taattggtct tcttattggt gaaaaaggta aatatggaat ttttgtttga taaaataaaa
     8341 aaagaaaaac caaaacagca tgaatggttt ctgttttggt tttttcgtta attttcatat
     8401 tcttctaatt ccaagcttag ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta
     8461 gcagcttcta aatcttggtt cagctcattg agttttacgt gatcttccag gttttctggc
     8521 gcgaccattt cttgttccaa ctgatcgatc cattcatcga tccgcgccat ttcttcttca
     8581 acctgagtaa tttttcgttg aaggctacgg ataagctttt gctgttcttt gctttggtaa
     8641 taatcagatt tgttcgtggg agcaggagca gattctggct catcttgtgc caacaattcg
     8701 gcgatttctt cttcttcctg ctttttctct aaataataat catagtctcc tagatagact
     8761 ttgctgccat tttcagagag ttcgacgact tttgtcgcga tccgattgat gaaatatcgg
     8821 tcatgggaaa caaaaagcag tgtgccgtca taatcgatca atgcattttc taatacttct
     8881 ttgttgtcaa tatctaaatg gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc
     8941 atcgctaact tggctaaagc aacccgtgct ttttcaccgc cacttagcag agggattggt
     9001 ttttctacat cttcgccaga aaataagaaa gcacctaaag cacttcggat ctctttttct
     9061 ggagtggtag gatgctcatc ccacaattct gctaggatcg tcttgtttgt atgcagatca
     9121 gcttgacctt gatcgtaata gccgatggtg acatttgtcc ctagcgtttc ttttccacgg
     9181 ataaaaggga tctggtgaat gattgatttc agcaacgtcg atttcccgat accattcgga
     9241 cctactagag caatcgcttc ttgacgacgg atatctagat tgattggttc agataaggtg
     9301 acattatcat atccgatagc ggcatcttcg atttgcaaga ctacatttcc agaaggcttt
     9361 tctatactga atagaaagtt tgcttgtttt tcatcccctt gaggacgatc cagccggtcc
     9421 attttttcta atgtctttct gcggctttgc gctcgttttg tggtagaggc gcgaacaagg
     9481 ttccgagcaa cgaagtcttc taatttatgg atttctgttt gctgcttttc ataagccttc
     9541 cattcacttg ctaactgttc tgctttcaaa tctagatatt tcgaatagtt acctttgtaa
     9601 tggcgcattt ttcttctgct taattcatag acttcattga ctactttatc taaaaaataa
     9661 cggtcatggg aaacaatcaa taatgcacca gtatagcttt gcagataatt ctcaagccag
     9721 gaaagtgtct cgatatctaa atggttcgtt ggttcgtcta gaatcaaaat gtcagggtgt
     9781 tgcaaaagca tgcgagctaa agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt
     9841 ttctgatcat aaaatgaaga atcgaattta aacccatgca atacggaacg aatttcattt
     9901 tcataaccat agccgtttcg atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa
     9961 taagaactat aagcaggtgt gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc
    10021 atccgctctt ccatttgtct aacttgttca aaagctttca gcatctcgtt ccaaactgtt
    10081 tcattggaat caagtcctgt gtcctgagcg aggtatccga gagtcgctgt tttattttta
    10141 gcgatctttc cttcatcagg tgcttcaatt ccagcaatga tctttaataa agtcgatttt
    10201 ccagcgccat ttctgccaac taaagcgaca cgagcgcctg tactgatttc taaatgtatg
    10261 ttttcaaata agacttctgc accaaaatgg cgagcaatct gattggcttg taataatatc
    10321 atggaatcct cttttctact ttgtgaattc tcctcaaata aaaaactatt tttcaatt
//
LOCUS       DAHEYX010000096        10353 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHEYX010000096 DAHEYX010000000
VERSION     DAHEYX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.097"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            6..305
                     /locus_tag="MB219_001638"
     CDS             6..305
                     /locus_tag="MB219_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8427355.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="MB219_001639"
     CDS             378..1739
                     /locus_tag="MB219_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCC8427356.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="MB219_001640"
     CDS             1761..2021
                     /locus_tag="MB219_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427357.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="MB219_001641"
     CDS             2035..2787
                     /locus_tag="MB219_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCC8427358.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="MB219_001642"
     CDS             complement(2881..3486)
                     /locus_tag="MB219_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8427359.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="MB219_001643"
     CDS             complement(3539..4513)
                     /locus_tag="MB219_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCC8427360.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="MB219_001644"
     CDS             complement(4891..5610)
                     /locus_tag="MB219_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8427361.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB219_001645"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="MB219_001645"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCC8427362.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="MB219_001646"
     CDS             7161..8558
                     /locus_tag="MB219_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCC8427363.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="MB219_001647"
     CDS             8639..9079
                     /locus_tag="MB219_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCC8427364.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="MB219_001648"
     CDS             9227..9553
                     /locus_tag="MB219_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCC8427365.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="MB219_001649"
     CDS             9554..10303
                     /locus_tag="MB219_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427366.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELIGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3287 a         1825 c         2145 g         3096 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg ataggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aag
//
LOCUS       DAHEYX010000097        10347 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHEYX010000097 DAHEYX010000000
VERSION     DAHEYX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.098"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            178..327
                     /locus_tag="MB219_001650"
     CDS             178..327
                     /locus_tag="MB219_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCC8427367.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            338..1852
                     /gene="dltA"
                     /locus_tag="MB219_001651"
     CDS             338..1852
                     /gene="dltA"
                     /locus_tag="MB219_001651"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCC8427368.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1849..3054
                     /gene="dltB"
                     /locus_tag="MB219_001652"
     CDS             1849..3054
                     /gene="dltB"
                     /locus_tag="MB219_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCC8427369.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3099..3332
                     /gene="dltC"
                     /locus_tag="MB219_001653"
     CDS             3099..3332
                     /gene="dltC"
                     /locus_tag="MB219_001653"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCC8427370.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3335..4603
                     /gene="dltD"
                     /locus_tag="MB219_001654"
     CDS             3335..4603
                     /gene="dltD"
                     /locus_tag="MB219_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCC8427371.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4744..5826
                     /locus_tag="MB219_001655"
     CDS             4744..5826
                     /locus_tag="MB219_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCC8427372.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5826..6629
                     /locus_tag="MB219_001656"
     CDS             5826..6629
                     /locus_tag="MB219_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCC8427373.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6699..7439
                     /locus_tag="MB219_001657"
     CDS             6699..7439
                     /locus_tag="MB219_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCC8427374.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7450..8454
                     /locus_tag="MB219_001658"
     CDS             7450..8454
                     /locus_tag="MB219_001658"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCC8427375.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8499..10343)
                     /locus_tag="MB219_001659"
     CDS             complement(8499..10343)
                     /locus_tag="MB219_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427376.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3336 a         1704 c         2088 g         3219 t
ORIGIN      
        1 atatttttta aaagtacgag aaattataaa gaaaaaagca gtgatttgat gtgctatttt
       61 tataaaagct atgtgacaaa actgttagta aaaaaacggt ttctgtaagc tagacacatg
      121 tttctttttg tcaaaattag ttacactttg tgtgaggtta tatgaggagg gaatccaatg
      181 aacaaatggc gttctaatcc aaaattgaat tattggggaa aattcatcat tcgaacaatt
      241 ttttatagtg cagtactgct gttcctgatt tatctgtatc actataaaaa tattcaagga
      301 ggaactttta tctataacga attttaggag gaagagtatg gaaatcaaaa cgattattga
      361 agcaatcgat gattggggaa taaatgaacc tgatcgagtt gcttatcagg cggatgagac
      421 gcatactttt ggtgaattga aacaagcatc agacgcactt gcttattatt tagaaaaaaa
      481 ggtagaaggc gatggaccaa ttgtcgtttt tggcaatttg gagtttgaaa tgattgtctc
      541 atttctaggg gttgtcaaat ctggccatgc ctatttgccg attgaagagc atacacccaa
      601 ggagcgtatc ctatcgatct ttcgggtggc taaaccatct atggtcatca gtatcgggga
      661 ctggatagaa gatattcctt cggttcctgt cataacaaaa aaagaatttc aagaaatcaa
      721 gaagatacca gtaggatttt atcccagaaa tagcgtaaaa ggtacagaaa atttttatat
      781 tatttttact tctggtacta ctggagaacc aaaaggcgta caaatcagtc atgataatct
      841 agtcagcttt gttcaatgga cgatggaaga ctttgggatc aagcctggta tgcatttctt
      901 agcccaagcg ccattttctt ttgatttatc ggtattttct atttatcctg cattagtttc
      961 tggcggaatg ctgaagccgt taaataaagc agttgttcaa gactttcgtc aattattcgc
     1021 gacattgcca gccttgaagt tgaatgtgtg ggtttctact ccatcattta tggatatttg
     1081 cttgatggaa cctacattca atggagaaaa tgtgccggaa ttggacatgt ttttattttg
     1141 cggagaagaa ttgacgaaaa aaactgcaga aagtctactt gatcgattcc caaatgcacg
     1201 gatatataat acttacggtc caacagaagc aactgtagct atttcaagta ttcagattga
     1261 ccagcatgta ctagatagtt atgaccgact accgattggc tacgtaaaaa aagacacgaa
     1321 agtttctatt gtccaagaag gtaaacctgt ttcaaaagga gaaacgggtg aaatcattat
     1381 tgcaggacca agcgtatcaa aaggttacct gaacaatccc gaaaagacag ctgctgcatt
     1441 cttttgtcaa aatgacttag caagttacca tacaggggat gcaggtagac tagatgaata
     1501 tgggctttta ttttatgaag gacgtatgga tcagcaggtc aaattacatg gataccggat
     1561 tgaactcggg gatatcgaac attatctgct ccaagacaac cgaatcaaac aagctgtcgt
     1621 cgtaccaaaa taccaaggaa caaaagtaca gcagctggtt gcttttgtcg tattagaaaa
     1681 caaaacgaaa cagccggatt ttcagttgac taaatctatc aaagaacaat tactagaagt
     1741 tgtaatggat tatatgattc ctcagaagtt ccattatgtc gatctgctgc ctcagacagt
     1801 taatggaaaa atcgatcgaa aaaaattaac agctgaggtg aatcctgaat gatgttacct
     1861 catatgatcc cttatgcaga gccgttttat ttttttcttt tagccattgc gcttgtaccg
     1921 atcgttctat ctttattgat atgggaaaaa agattaccag tttatcaatc agcagtgaca
     1981 cttttctttt tatttgtcag ctttggaggt aattattggc aacagggagc ggcgctgatt
     2041 gggtatgttt tgtggcagac gatacttgta tggggctatt ttgtttatcg gcaaaggaaa
     2101 aatcagacag gatggttttt tgccgccgtt attttagcga taattccact gtttttaacg
     2161 aaagtgactc ctttttttga acatggaaaa gcatcgttac ttggtttttt aggaatctct
     2221 tatttaacct ttaaatctgt acaaatgatc atggaaataa gagatggcat gatcaaaaca
     2281 tataagccat ttcgttatat ccagtttctg ctgttctttc caacaatctc ttctggacca
     2341 atcgatcgct ataggagatt tgaaaaagat cagctccatc cgcctgttcg tgataaatat
     2401 gttgatcttt tgggaaaagg cgtccaaaat atttttgtcg gttttttata taaattcatt
     2461 atcggttatt attttggtca agtactgctt cctgtagtgg gacgcattgc cttacagcat
     2521 ggtggtattt catgggcatt agtaggttat atgtacgtgt acagtatgta tttgtttttt
     2581 gattttgctg gctatagctt atttgcagtc ggtacaagct acatgatggg atatcagaca
     2641 ccaatcaatt tcaataaacc ttttttatca tggaatatca aagaattctg gaatcgttgg
     2701 catatgacgc tgtccttttg gttccgtgat tatgtatata tgcgattgat gttcttcatg
     2761 atgaagaaaa aattttttaa aagcaagatc gtaatgtcaa atatcggtta ttttgctttg
     2821 tttcttctaa tgggtatatg gcatggattg acgtggtact acatcgtata tggactgtat
     2881 catgcttgtc tgatctgtct gacagatgca tggatccgat ttaagaagaa gcataaagag
     2941 cagttgcctt ctaacaaatt tacgcatggc ttagccgtat ttttgacatt tcaagcagta
     3001 tgttttagct tcctgatttt ttcaggattt ttagataaac ttttttttag ctaacgaata
     3061 gtgacatatt aatttatata acaaaggaga aaaggaaaat ggaagaacaa attttatcaa
     3121 tcttagaaga aataacagga acagatgaag taagaaaaaa tcgtgatatt gatctatttg
     3181 atgaagggtt gcttgattct ttagcatctg ttcaattatt agtcgagcta gacggagagc
     3241 tggatatcca agtccctgta tctgaattcg agcgagagga ttggagcaca ccgaataaaa
     3301 tcattcaaca agcaactgca ttgaagggtt gagtatgaaa aagaaacttt ttgctatttt
     3361 cggcccggtt gttttagctg ttggcttact gtttgtgttt tttacctcct cctatcaagt
     3421 agatatctct aatccagaaa cgattagacg tgcctcttct tccatgtcag tgaacgtgtt
     3481 gaaaggagat gcgataaaaa attatgcatt atctgaaaaa caatatattc ctttttttgg
     3541 ctcaagtgaa ttaagcagaa tcagtccatt tcatccgtct gttctggcag aaaaatacca
     3601 gcgaaactat cgtccatttt tgctgggagc acccgggaca caatcgttaa gccaatatat
     3661 gatgatgcgt tctgcaggag atgctatgaa aaacaagaaa gtcgtcttta ttatttctcc
     3721 tcagtggttc gttaaaaatg gtgtaaaaac tgattacttt aatacgtatt attctgaact
     3781 tcaaacatac gactggttgt tttcaatgaa gaaagtaacg cctgctgatc gctatttagc
     3841 tagaagatta ttaactttct caaaagtaaa agagaatgat acgttgacgg cgattctgca
     3901 aacgatcaaa aaagggaagc tacctttacc tgaatctttg aatcagctgc gcagccaatg
     3961 gaacatgctg aagagagagg atgaagtatt cagcaatatt gggttgaaag atcgacagca
     4021 aaaaatcgat catgagtcaa aacgattacc aaaacaatac caagaaacag aattatccat
     4081 tttagcaaat caaattggag aacgagaaac gacgaataat ccattcgggt tgaaaaacga
     4141 tttttatacg catcgtatcc gagcccacga gccagagctc aaacagtcac aaaaaaattg
     4201 ggattatcgt ttttcgccag aattttctga ttttcagctg gtcttggatc aattagcaaa
     4261 aaaccataac gaagtattgt tcatcatccc accggttaac gagaaatgga gcgattatac
     4321 cggactatct caagaaatgt tacaaggatt tgccaaaaag atcaaatttc aattaaatag
     4381 ccaaggattc aatcgaatag ctgattttgt caatcaagcg ggtaccaact attttatgga
     4441 agacacgatc catctaggct ggaaaggctg gttggctgca gatcaacaga tcagaccgtt
     4501 tttagaagaa aatcatatta ctgctagtaa gtatcatctt gacgatgctt tttttagcaa
     4561 atcttggcaa catcaaatac cagataaact acaattaaaa taaagaaaac aggcgagtgt
     4621 tgctcgcttg ttttctttat ttttgaatat tctgaataaa agataaaaat accaagtttg
     4681 cctgaacaaa aatcataagc aatggtataa tcacaatatg aatatagaaa tgggggaaaa
     4741 catttggata caaaagaaga acagctactt attgatttaa cttctgcaaa aggagtacct
     4801 ggaaatgaag aagaggtaag agaagtattc agagaatatg ccaaaccatt cgctgatgat
     4861 attttttatg acggtttagg cagtgtgatt gccaaacatg gtgctggcgg aggccccaag
     4921 gtctttattt ctggacatat ggacgaagtt ggttttatgg taacaaaaat aaccgaaaaa
     4981 ggattcttgg aattccaaac tcttggaggt tggtgggggc aagtcatgct tgctcagcaa
     5041 gttgaaatca aaacacgtga agggaaaatc gtccacggtg tgattggttc caaaccacca
     5101 catgttttaa cacctcaagt aagaaataaa ccttatgaaa taaaggatat gttcattgat
     5161 attggcgcat caagccaaga agaagccaaa gagtggggca tacgtcctgg agatatggtc
     5221 actccatata ttgaatacaa acgaatgaat ggcagtaaat atttactggc aaaagcatgg
     5281 gataatcgta tcgggacagc tgtttctctg cgagtattag agaatctatc taaagaagca
     5341 cacccaaatg ttttattcgc aggaagcgat gtgcaagaag aagtaggctt gcgaggtgct
     5401 cgaacaagta cacaccttgt aaatcccgat attgcttttg ctttagatac tggtacagca
     5461 ggagacactc caggaatgac accaaaagaa gcagattcga ttttaggaaa aggcccacag
     5521 attttgattt ttgatgcatc tatgatcccg cataaaaaat tattgaattt tgttattgat
     5581 gtagcagagg aaatggatat tccattccaa tatacagtga tttctggcgg aggaactgat
     5641 gcaggacaaa tgcatttgac aagagatggt gttccgtctg tagccattac cgttcctgtt
     5701 cgttatctgc attcccatac atcagtgatt catgaagatg attatttgaa tacagtgaaa
     5761 ctagtcacag aattagtgaa acggcttgat agtgaaacag ttgaaaaaat tcggagttat
     5821 gaataatgaa taatagaata cctatttctg aatatcggaa aaaagcacga gtttctttgg
     5881 aaaatcaatg gggaatcaat acttggcttg ttttcttatc tataatattt acgagcgtca
     5941 tccagaactt ttttgacggt ttcagtcctt ttccggaaga aagcatgcag cagcacttat
     6001 tgagttttct agtcgataat ttacttttat tcgcattcaa ctatgcattg ttttacattg
     6061 cgctagtagt tgtacggggt ggcagaccaa aaagtaacct tttattttca gttttccaaa
     6121 gaaagtattt tggttcgata cttggcatca atttgatcaa ttcagtcatc aactggctac
     6181 tcaacagttt aatctttctg cccatgtttt taatcagcgg agtgagtact tatatgcagc
     6241 tgcttttcag ttcaaatgcg gtacctacgg gtgtcatgcc agaagatttt cttgatatca
     6301 gctttgtttt atcgatgata gggatgattt ttgtttcagc actcatcatg agtattgttg
     6361 gcggtttatt ccaatttgct gcatggacaa agttcgacta tcctgatttg tcggttattc
     6421 aatgtttgaa atatggctgg tttttattga aagatagaat tggtacgtat attttgttgc
     6481 agctgtcttt tatcggctgg tatttgctag gagcaatcgc tttgttcatc ggtcttttgt
     6541 gggtcattgt ttatgtgaat gtgtcagttg cagaatttta tgaacaagca cggattgaaa
     6601 aaggaggtcc agacgcctat tttacttagc atgtaagcag tttattcatc aaaaaaataa
     6661 cttttgtggt aggataaatc tttgaggggg aatgtacgat gaaattaact gttttaggat
     6721 gcttaggagc gtatccttat aaaaatcaag ggacaacagg ttatttgctt caatctgacg
     6781 ggttcaattt gttgatcgat gcaggtagtg ctacgctgat caaattacaa gaacaccttg
     6841 atccacttga tctagatgct gtgatcctta gccattatca tcatgatcat attgctgacc
     6901 ttggtgtatt acagtattac tggcaacttc atcctaagag aaatccggag acattgcttt
     6961 caatttatgg acacacaaaa gattccattc actttgaaga attgacgttg gaaaatgtct
     7021 caaaaggaca tccttatttt gaagctgaag aattaaaaat tggtcctttt ctgattactt
     7081 ttatggaaac aatccatcca gtagtatgtt acgccatgcg tatcgtagaa gaaacaacgg
     7141 ggaaagtatt cgtatttaca ggtgattcag gttatttgga aagtttcgct gattttgcga
     7201 aagatgctga tttgtttcta gcagatacct atctttttga aggaaatgaa catcatcatg
     7261 cccatttcac ttcaaaagaa gctggagaac ttgcgaaatc agctaacgta aagaaacttg
     7321 tacttacaca cctacctcag tttggtgatt tagaacaatt gaaaaatgaa gcgcaaaagg
     7381 cagcaggcaa agggattgat atagaacttg ctgcagttga caaagtgttt aacatttagg
     7441 aggaagaaca tgatttttgt accaaatgaa aaccacgacc caagaatcaa tttagcaatt
     7501 gaacaatatt tgctgcaaga aatgccttta gatgaaccta ttttgctctt ttatatcaat
     7561 gaaccttcaa tcatcatcgg cagaaaccaa aatacgcttg aagaaatcaa tcgagattat
     7621 gtggaagaaa atggcattca tgttgtccgt cgcttaagtg gaggcggagc tgtctaccat
     7681 gattttggaa acctgaactt tagttttatc atgcctgatg atggggattc attccgtgac
     7741 tttgcgaaag taacacagcc aatcatccaa gcacttcatg aattgggtgt tgctggggcg
     7801 gaattgaaag gccgaaatga tctagtcatt gatggcatga aattttctgg caacgcgatg
     7861 tatgctacca acggtcggat gtttgctcat ggaacaatta tgttcgactc tgatatcaat
     7921 gaagtagtca atgcattaaa agtaaaaaaa gacaaaatcg aatcaaaagg aatcaaatcg
     7981 atccgttctc gtgtaacgaa tatcaaacca tttcttccag aagaaaagca aaaaatgaca
     8041 acagaagaat ttcgtcaaga aatcttgttg aaaattttcg gcgtagagtc agtagaagaa
     8101 gtaaaaacgt acgaattgac tgatgaagat tggaaaaaaa tcaatcgaat ctctgaggaa
     8161 tattatcgaa attgggattg gaattacggg aagtctccag atttcaattt ctctcgccaa
     8221 aaacgttttt ctatcggttc gattgaagtc catttgaatg tctcagaagg aatgatcaaa
     8281 gaagcaaaaa tctttggcga tttcttcgga ctaggagata tctctgacgt ggaaaaaaaa
     8341 ttggttggac agaaatatga taaagcatct ctatcaaaaa tagtcagcca aattgatgtg
     8401 aagaaatatt ttggtgcgat agaaccagaa gaattgctta ccttacttta ttgaaaataa
     8461 aaaatagcgg aatccacata tatggattcc gctatttttt atacaggatt cgggtaattt
     8521 cgagagagtt gaacagtgat ccgatcaatg aaagtatctt tttcctgcgt tagtgttcct
     8581 gtgtaacgat ccgctacgtc tttcatcatc attaagtcaa aacgttcctg atcatctaat
     8641 tcttctgtga tgcctcgact aaataggttt gtatcgtaat gtgcactatg ttcgcaacaa
     8701 atgacgaaat gttcctcttg ttcgtgtatc gacaaataaa tttttcgata cagtggattt
     8761 tcgatggcat gagtttctct aaaggaatgc tctaaagcgt ggatcaaaag ttgagccaga
     8821 tcgtcatcgt tgataggaag ttcttcggga agatctgctt ggaaggttat ctgaatattc
     8881 cgtttggcag ctaacttctg gtaacgcgct aagataagat ttgtcacctg gtggttgcaa
     8941 aaagaacaca gcatttcgaa attttttgct tcttctaata tcttttccac atagttttta
     9001 gcttttgttt ggtccgtatg cattaaatct tgcagattct ccatctcttg gacaaattcc
     9061 aggcgaagtg tatttacttc ttggatatgc tgtcgaagct gctcataatg ttgctccaac
     9121 aaatcatttt tgatttttaa gaaatctact tgtctccgat ctttgtcggt atttaagatg
     9181 tgatcgatta gattgtagct gataataacg attagaatga taatatataa aatcgtagac
     9241 caattgatcg ttgtttgata gatacctaat gccgtttgga tgtaaatcaa gcaatggata
     9301 atggctacca agacgatcca aggtgtacaa gctacataaa aacggttatt cttatatgct
     9361 tcgccaatgc atgcaagtga agtgactaaa gtgcttataa cactagcaat attagcaatc
     9421 ggcattaatt caggaagttc tgcaggagta aaagttgcat aaagcagaac gccgataaaa
     9481 atcaaaagtt ggaacgtaca aaaaattccg taccattttt tatagtaaaa cattcggctc
     9541 caatagtagc agatgatgaa agctgacgtt aagataataa ataaatgaga taaaaaagaa
     9601 tgaacgaatg gttcaaaaag aataccactt aaaatatctt ctgatacagc ttcaaatcct
     9661 aatgccaaag caaagcaact taagagaata agtgagtatt caagacgttt ttgtttatac
     9721 atcaagtaga tcgtatagaa aaaggtccct atagcgataa aaacagcgat tatcatcgtc
     9781 aatacttgcg gcactgattg tgaataaata aaactaatca atggtccagt tgttcctaga
     9841 taaactttcg gtggcagtcc agcatagttc tgataaggag atgagactgt aagggtcaat
     9901 ttttttccga tataatttgc tggtaaatct atcacagcca atgagagtcc gggattgctt
     9961 tcttcctgat aatctttccg ttggtataaa atcttttcgc ctagatatac agtaatccat
    10021 tgatggtttc ctttgattag taaatttgct tcgtccaacg gtttttcaag tgtcgtttcc
    10081 ataatgaatg tttcgtcttt atccacggta ggaatcgtac gagtgtactt gctgtcaaac
    10141 ttaaaatcag gatcatcttt tgtatagaaa acccacgaat cttttatttc tttcatttga
    10201 ttgctgtccg caggagaaaa aataaaataa aagaaataag caattcctgt taaaaataac
    10261 aatgataata taatgattat gctccacttt atacgtaaat aaaaagattc ttttttatta
    10321 tatatgttat taaaaaaaga cataaag
//
LOCUS       DAHEYX010000098        10340 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHEYX010000098 DAHEYX010000000
VERSION     DAHEYX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.099"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(294..1142)
                     /locus_tag="MB219_001660"
     CDS             complement(294..1142)
                     /locus_tag="MB219_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCC8427377.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1141..2208
                     /gene="gnd"
                     /locus_tag="MB219_001661"
     CDS             1141..2208
                     /gene="gnd"
                     /locus_tag="MB219_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCC8427378.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2210..3211
                     /locus_tag="MB219_001662"
     CDS             2210..3211
                     /locus_tag="MB219_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCC8427379.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3208..3492
                     /locus_tag="MB219_001663"
     CDS             3208..3492
                     /locus_tag="MB219_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCC8427380.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3525..4322
                     /locus_tag="MB219_001664"
     CDS             3525..4322
                     /locus_tag="MB219_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCC8427381.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HVAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4336..5169
                     /locus_tag="MB219_001665"
     CDS             4336..5169
                     /locus_tag="MB219_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8427382.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5187..6044
                     /locus_tag="MB219_001666"
     CDS             5187..6044
                     /locus_tag="MB219_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCC8427383.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6066..6575
                     /locus_tag="MB219_001667"
     CDS             6066..6575
                     /locus_tag="MB219_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8427384.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYSVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6612..7013
                     /locus_tag="MB219_001668"
     CDS             6612..7013
                     /locus_tag="MB219_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427385.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7125..7478
                     /locus_tag="MB219_001669"
     CDS             7125..7478
                     /locus_tag="MB219_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427386.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7512..7955
                     /locus_tag="MB219_001670"
     CDS             7512..7955
                     /locus_tag="MB219_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCC8427387.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7972..9057
                     /gene="uxuA"
                     /locus_tag="MB219_001671"
     CDS             7972..9057
                     /gene="uxuA"
                     /locus_tag="MB219_001671"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCC8427388.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9050..9763
                     /locus_tag="MB219_001672"
     CDS             9050..9763
                     /locus_tag="MB219_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCC8427389.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9970..10119)
                     /locus_tag="MB219_001673"
     CDS             complement(9970..10119)
                     /locus_tag="MB219_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427390.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3458 a         1636 c         2243 g         3003 t
ORIGIN      
        1 aggaaatact taattcacca aaattttttt taatttttta gaaaataaga aacaaaaata
       61 ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta tcgcatctaa
      121 caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca tacgttctaa
      181 cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata
      241 tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg gtgctattct
      301 ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc tttttcgttg
      361 taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat acgggaagct
      421 aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac atcgccaagt
      481 ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc agtttttctt
      541 acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat caaaacagca
      601 cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc cgtgctgtga
      661 gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac aatctctgaa
      721 ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc cgcttttttt
      781 agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact agactcgaac
      841 accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg gatagaaata
      901 gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa tccattataa
      961 ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa
     1021 ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc ggcgatagcc
     1081 tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat ggagacgctc
     1141 atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat taatgttaaa
     1201 gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa
     1261 aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga aattggtgta
     1321 attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca ccatcacaaa
     1381 gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa tgacattcct
     1441 gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat catctggtta
     1501 atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc aaaactcgga
     1561 ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat ccgtcgagca
     1621 gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg aggaatcgaa
     1681 ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg
     1741 gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga
     1801 agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc
     1861 ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca
     1921 cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag
     1981 ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca
     2041 aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt
     2101 ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca
     2161 ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta
     2221 tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag
     2281 attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga
     2341 actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg
     2401 acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta
     2461 caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc
     2521 tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg
     2581 acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt
     2641 cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag
     2701 cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta
     2761 caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact
     2821 ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat
     2881 ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg
     2941 cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata
     3001 tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga
     3061 acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg
     3121 tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt
     3181 ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg
     3241 aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc
     3301 ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag
     3361 gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat
     3421 tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa
     3481 tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca
     3541 atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga
     3601 cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt
     3661 acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat
     3721 atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag
     3781 aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct
     3841 gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac
     3901 tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag
     3961 gattaggaga taaatacgca agaaatgcgg cagcacatgc tatgcatgtc gctatcagta
     4021 aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct
     4081 atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg
     4141 aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa
     4201 tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg
     4261 taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct
     4321 gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg
     4381 gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct
     4441 cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt
     4501 gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca
     4561 gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg
     4621 ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag
     4681 tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg
     4741 aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta
     4801 ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc
     4861 aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg
     4921 gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc
     4981 ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat ggttacttat
     5041 gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta
     5101 cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac
     5161 ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc
     5221 ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg
     5281 aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa
     5341 tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac
     5401 tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta
     5461 tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact
     5521 tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg
     5581 attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa
     5641 gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg
     5701 tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta atgcaaaaca
     5761 ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc
     5821 ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt
     5881 ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa
     5941 tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc
     6001 tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga
     6061 ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa
     6121 tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag
     6181 caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa
     6241 caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat
     6301 acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa
     6361 tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt
     6421 tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg
     6481 aacaatatag cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat
     6541 taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag
     6601 agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata
     6661 tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag
     6721 atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat
     6781 caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg
     6841 atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa
     6901 aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa
     6961 tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta
     7021 tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc
     7081 ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc
     7141 gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa
     7201 agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact
     7261 ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt
     7321 ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa
     7381 aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt
     7441 ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag
     7501 tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga
     7561 aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt
     7621 ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca
     7681 cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc
     7741 tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg
     7801 tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag
     7861 aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag
     7921 cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg
     7981 ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata
     8041 ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag
     8101 atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga
     8161 attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat
     8221 attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt
     8281 tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat
     8341 ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg
     8401 tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg
     8461 ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt
     8521 gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa
     8581 atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa
     8641 aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg
     8701 ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag
     8761 gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag
     8821 ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa
     8881 gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg
     8941 ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg
     9001 caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat
     9061 caagctcttc atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg
     9121 tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt
     9181 actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga
     9241 tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga
     9301 agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag
     9361 aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa
     9421 aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg
     9481 aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga
     9541 agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt
     9601 gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt
     9661 gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca
     9721 tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc
     9781 tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt
     9841 ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc
     9901 tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt
     9961 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
    10021 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
    10081 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
    10141 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
    10201 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
    10261 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
    10321 aatagaagac aatgaatttt
//
LOCUS       DAHEYX010000099        10137 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHEYX010000099 DAHEYX010000000
VERSION     DAHEYX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.100"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            121..627
                     /locus_tag="MB219_001674"
     CDS             121..627
                     /locus_tag="MB219_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCC8427391.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="MB219_001675"
     CDS             644..1543
                     /locus_tag="MB219_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCC8427392.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="MB219_001676"
     CDS             1630..2514
                     /locus_tag="MB219_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCC8427393.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="MB219_001677"
     tRNA            complement(2706..2777)
                     /locus_tag="MB219_001677"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="MB219_001678"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="MB219_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCC8427394.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4320..5411
                     /locus_tag="MB219_001679"
     CDS             4320..5411
                     /locus_tag="MB219_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCC8427395.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="MB219_001680"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="MB219_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCC8427396.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="MB219_001681"
     CDS             6563..7753
                     /locus_tag="MB219_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCC8427397.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="MB219_001682"
     CDS             7857..8612
                     /locus_tag="MB219_001682"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCC8427398.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="MB219_001683"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="MB219_001683"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCC8427399.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHHLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1870 c         2100 g         2619 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagct gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggtggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcaaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgcggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 catttttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 caccacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgactca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agatggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaatgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagtttt
//
LOCUS       DAHEYX010000100        10117 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHEYX010000100 DAHEYX010000000
VERSION     DAHEYX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.101"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB219_001684"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="MB219_001684"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HCC8427400.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB219_001685"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="MB219_001685"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HCC8427401.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB219_001686"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="MB219_001686"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HCC8427402.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="MB219_001687"
     CDS             complement(4028..5044)
                     /locus_tag="MB219_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8427403.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="MB219_001688"
     CDS             complement(5146..5343)
                     /locus_tag="MB219_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427404.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="MB219_001689"
     CDS             complement(5315..6634)
                     /locus_tag="MB219_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8427405.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWQERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="MB219_001690"
     CDS             complement(7375..8355)
                     /locus_tag="MB219_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCC8427406.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="MB219_001691"
     CDS             complement(8368..9246)
                     /locus_tag="MB219_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCC8427407.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="MB219_001692"
     CDS             complement(9262..10062)
                     /locus_tag="MB219_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCC8427408.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         2729 a         2348 c         1715 g         3325 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttgccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattctt
//
LOCUS       DAHEYX010000101        10065 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHEYX010000101 DAHEYX010000000
VERSION     DAHEYX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.102"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            101..1057
                     /locus_tag="MB219_001693"
     CDS             101..1057
                     /locus_tag="MB219_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCC8427409.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1130..1246
                     /locus_tag="MB219_001694"
     CDS             1130..1246
                     /locus_tag="MB219_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCC8427410.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1301..2983)
                     /gene="recN"
                     /locus_tag="MB219_001695"
     CDS             complement(1301..2983)
                     /gene="recN"
                     /locus_tag="MB219_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCC8427411.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3001..3450)
                     /locus_tag="MB219_001696"
     CDS             complement(3001..3450)
                     /locus_tag="MB219_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCC8427412.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3557..4375)
                     /locus_tag="MB219_001697"
     CDS             complement(3557..4375)
                     /locus_tag="MB219_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCC8427413.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4384..5274)
                     /locus_tag="MB219_001698"
     CDS             complement(4384..5274)
                     /locus_tag="MB219_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCC8427414.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5276..5500)
                     /locus_tag="MB219_001699"
     CDS             complement(5276..5500)
                     /locus_tag="MB219_001699"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCC8427415.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5505..6842)
                     /locus_tag="MB219_001700"
     CDS             complement(5505..6842)
                     /locus_tag="MB219_001700"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCC8427416.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6843..7697)
                     /locus_tag="MB219_001701"
     CDS             complement(6843..7697)
                     /locus_tag="MB219_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCC8427417.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7794..8243)
                     /gene="nusB"
                     /locus_tag="MB219_001702"
     CDS             complement(7794..8243)
                     /gene="nusB"
                     /locus_tag="MB219_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCC8427418.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8236..8661)
                     /locus_tag="MB219_001703"
     CDS             complement(8236..8661)
                     /locus_tag="MB219_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCC8427419.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8907..9971)
                     /locus_tag="MB219_001704"
     CDS             complement(8907..9971)
                     /locus_tag="MB219_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCC8427420.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
BASE COUNT         2820 a         2242 c         1659 g         3344 t
ORIGIN      
        1 ttaaatttat gcagttatat tctatatctt acaaacacat tccatgtatt ccaattccct
       61 atccgttata ataaaagaaa caggcaacag gaggaaaatc atgatcaaat attttacatt
      121 agataatgag aaactgcgta ataccggaaa ggaaactgac caaacgatct ggtattgtgt
      181 agaacgtcct aatcaagaag agattcatca gctgacacat caatttgaaa ttccgtcaga
      241 ttacatcact tccatattgg acgatgcaga aaattcccgt acagaagaat tcaatcaatc
      301 aaagttgaca aagccagcgt tattattgtt acaatatcct tttgctaaaa caagtcctag
      361 cggttatttt caagtggata catacccgct ctctattatt ttgacgccca aaaagaagct
      421 tatcacaatt acgaatcatg aaccactgtt tttaaagaaa gtctttagcc agacctttcc
      481 aaaaagtgat atttcgccta atctcaatat cttctttcaa ttcatttggc aggtcacact
      541 ctcttacaac agttatttag ctacgcttca atctcaatgc gatactttgg aagccaaact
      601 tcaagtatcc actgaaaaca aacaacttta tcaaatcatg gatatccaaa aaagcctcgt
      661 ttatttcaga gaagcgactt ctgctaattt agatgcattg aaagaactag cgcaaaataa
      721 acttttccaa gaaaatcatg ctttgaaaaa tcatttacgt gatgtattgg tggaaacaga
      781 gcaggcaatg actacttctc gcattcaatt aaaactagta gaacaaatga atcagacttt
      841 ttcagcaatc gtatcgaata atctgaataa tattatgaaa attttaactt ctttaacgat
      901 tattttaact ataccgacaa tcgtcggcag tatctatgga atgaacatta agttacctat
      961 tgctgaaagg gatgatgcct ttatatggct agttctatta acattattta tttgtatcgt
     1021 aactacctat tatttaaaaa aagggaagtt tttataaaaa agccctcttg tggaaatgga
     1081 aaaaagattg tacacttgaa tcaactaatt aatgacgagg ttgaaaacaa tgcgtgaaaa
     1141 aaaacaaaca agtacttttg ctaaagtaac aaaatttgtc atttggacaa tgctgatttt
     1201 aacgattgga tctgttgtat tgactgctgt aatgagtgtc atgtaaaaat aaaatatagc
     1261 tatcagctgc tgaaaaggac ctgaaccact gcgaggtggc tcaggtcctt tttgcgagtt
     1321 tcaatagttc ttttgcatgt tcgagtgtga gttttgtaat ctcacttcca gccagcatac
     1381 gtgcaatttc ttcttctctt tcttttgttt tcaactctga aacagaagtt tgcgtacgtc
     1441 catcaatcac tgctttcaca atataatatt gatagtcggc gactgcagca acttgaggca
     1501 gatgagtgat gcacaaaact tgagagtatt tcgatatttg agaaatttta tctgcgatgg
     1561 cctgtgcaac tcttccgctc acacctgtat ccacttcatc aaaaataata cttgtcacac
     1621 cttgctcaga agaaaaaata gttttcaaag caagcaacat ccgagacagt tcgccaccag
     1681 aagcgacttt caccaaaggc ttcagtggct ctcctggatt ggtcgtaatg taaaattcga
     1741 tttggtcaaa tccatcatga ttcaattgtc tattttcttc ttttaagaaa cgtacctcaa
     1801 actcagtgtt ttccatatac agacttttca attcatgcag aatggatttt tccaattctt
     1861 ttgcgatttt tttacgcgct tcatgaagat ctgcggcttg ttgctgaagt agaagctctt
     1921 tttgctctag ttcctctttc attttgtcca attgaccttc tgaaaaatca gaagaagcta
     1981 gctcttcggt gatttcgtca tagtaagcca atattgactc aatggattcg ccgtatttac
     2041 gtttcaactg tcggattaat tctaaacgtt gcgtaacttc ttctaaacga ctctcatcta
     2101 actccaataa atcaatctga cgactcatat cgccgattgc atcttgcagc aaatagtaag
     2161 cactttggat attttcataa attgcttcat actccacatc tagatgggcg atcccttgca
     2221 gttctgaaac ggccaagcca acaccatcta cactgttttg ttcattattc gtcagtgctc
     2281 cgtaacctgc agctaatcca tcagcaattt tttgataatt gctaagtttt tctctttctt
     2341 cttttaattt ctcttcttct ccgacttgca aatcagcagc agctatttct tcttgctgga
     2401 aatggagcat atctattctc tgaacatagg atttttcatt ttgctgcact tttcgtaccc
     2461 ttttttccag ctcacgataa gagatatatt cttgctgata actctttttc ttctgttgga
     2521 aagctacatc tccaaaacga tccaataaag caagatgtga ttcaggctgg agcagttcct
     2581 gatgttcgtt ttgtccttgg atatctacta aataactgcc gattcttctc agattagcta
     2641 atgtgataat atgaccattc actcgacaaa cattttttcc agttaaggac atatcccgtc
     2701 gaacaatcag attgtcctca tctgtttcga tccctaattc aaccatcagt tcagagaatc
     2761 cttcttgttt tggtagttca aacaaccctt caagcacaca tttttctgct ccttgacgta
     2821 tataatcgct tgaacctctt cctcctgcta acagacctaa agcatcgata ataatggatt
     2881 tacccgcacc agtctctccg gtcagcgcgg tcattccttc gtgaaaagaa agccgcaatt
     2941 ccggaataat cgcaaagtta gtaatactga tttcttgtag catcacattc gctcctcaac
     3001 ttaaaggtaa tgcaggaagt ctttcttcaa taaaactgcc tggtcttcag ttcgtgtaat
     3061 catcaaaaca ttatcgtcat cattgataat cgaaaataat tctttcttgt aacgtttatc
     3121 aatcaaattt gctgctgccg aagcatttcc cggcaatgtt ttcaaaataa caaatttttc
     3181 catttggtca actgccacga aagcgtcttt taatagtttt tccaatttag tatttacatc
     3241 ttcgtttgtt tcagctggta tgctataacg gtagccgcca tctgaagccg gtaccttgat
     3301 cagtttcagt tcctttatat ctctggaaat cgtagcttga gtaacagata ttcctttgct
     3361 tttcaggatt tcaacaaatt cttcctgctt tcggatatct tgctcactca acaatcgagt
     3421 aatcaaacga tgccgatctt tttttctcat tacgtcttcg ctccttagca tattcttctt
     3481 aatcaatgat agcttatttc cttggtataa taaagaagaa tccgcatact tctcacttga
     3541 ttttcacagt tacaatttat ttatggtcaa actgttcgtg tgcatttgaa accacttctg
     3601 ccatattaat agctgaattg attgttccgc tttcgggtac tttcctcaag tgagccaaga
     3661 actcgatatt tccttctccc cccgtgatcg gagaataact taattccagc acatcatatc
     3721 caccttgtgc cgcaaatgac aaaatgtctt ctaaaacttt ttgatgggtc tgagggtcac
     3781 gaacaattcc gtttttccct acagcttctc tgcctgcttc gaattggggt ttgattaaag
     3841 caatcacttc tccctcttct tttaaaatag catgtaatgg aggcagcatc agttttagtg
     3901 aaataaaaga aacatcgata acagcaactt ctggaattcc taaggtaaag tcttcgggtt
     3961 ttgcataacg aaaattcact ctttccatta cgacgactct aggatcttgt cttattttcc
     4021 aagctagctg attgtagcca acgtctaaag cataactcat ttttgcgcca ttttgcaaag
     4081 ctgcatcagt aaatccgcca gtggatgcac caatatctaa caatgttttt ccttcaacag
     4141 atagatcaaa tgctttgagt gctttttcta attttaatcc ccctcgagaa acataaggaa
     4201 gttttttacc tttgactctt aattcactac tcactggaat tttttctccc ggtttatcaa
     4261 accgctcgtt tttttcgttg taaatcaaac cagccatcac tgaacgtttg gcttgttctc
     4321 ttgtttcaaa taatccttgt ttgaccgcta atacatctac gcgttctttt tccattcttt
     4381 ccctcatttc tccacatcaa attgcttgat catctggcgc atcagcccgc catcaaaaga
     4441 aaaaacgtcc tcttctaatt tgtctatgat cttgttggcg gataacagct caatctccaa
     4501 cgcctcacgt gtcttttcta ggcctaataa acgcgggtaa gtattttttt ccatacgttc
     4561 atctttacca gctgttttcc ctaatgtttt cgttgtacca atcacatcga gtagatcatc
     4621 tcttatctga aaagctaaac ctaaatgttc ggctaatcgt tgaagtagca ataagatttc
     4681 ttcaggttgc ttcgctaata tgccaccagc cagcaaggca taacgaatca aatgccctgt
     4741 ctttctttca tgaataaatg ctaattcttc taaagaaagc tttttcgatt cgccctcgat
     4801 atctgcagct tgcccagcaa ccataccttg tgatcccgca caaacagcta attgctgaag
     4861 cagcagaagc ttaggagaat tacctaagtg tgccatactg attagctgga atgctcctgt
     4921 aagcaaacca tctccggcca atatagctaa tgcttcgccg aatactttat gattggtagg
     4981 ttttcctcta cgaagatcat cattgtccat cgcaggcaaa tcatcgtgga tcaaggaata
     5041 cgtatgcacc atttctaaag cagctgctac ttgatagacg ggaacatcaa taggctcatc
     5101 aaaggaagca acggccgtta aaagtaataa gggacgtatt cttttcccgc ctgctttcac
     5161 tgaataactc atcgcatcat agagtcctct atccgttgta tgctcatcta aaaaagacaa
     5221 gatttctttt tctacagcag gtagatgaat agctgaaaag tcagttaatc tcatctcagc
     5281 tttcctcgtt tccgtcaaaa tcaacttctt catttgattc tgtcatcatt ttcgtcaagg
     5341 tctcttctgc tttagctaat gtatcttggc aatgtttgct taattccatt cctcgtttaa
     5401 acgaatctag agctgattct aatggaacgt ctccttgttc taactgcatg acaatttttt
     5461 ctaattcttg cagtgactct tcaaaagttg gattttccat atttttattc tcctttgtct
     5521 atctcttgta cttgtccttt aacgattcca tcttgataat ggatcgtcag caaatcccct
     5581 tgatgaatct cggtgacact ttttactact tgatcattta acgtcgtata gctgtagcct
     5641 cttcccatga ttttcaatgg actcaacaaa tcaagagatt gaacagcttg agcaaaacgc
     5701 tgctgctgtt gtttcatata ttgttggatt ttctcttcta agcgtttttc tagataagca
     5761 gtttcttggc gagctgcttt aactcgataa agcggagcca cttgttccaa ttggtggctt
     5821 aatgcgatcg tttgtttttc tttcgcatga taaatttgct gtgtagcttg atataatcgc
     5881 tgtttcaact ggtctaattt gatggtctga ccttcataca atctttcagg ctgccgaaat
     5941 acataggaac gtcgagcacg ctcgaaccgt tcttgtttgc gctggatctg gcgtacaaac
     6001 gcctgctgca atctagcttg ccgttcttga atgcgcatca actcttctga caaaacagga
     6061 acagctaatt ctgcagctgc tgttggagtg gcagcccgaa catctgctac taaatcagcg
     6121 atcgtggtat ctgtttcatg tcctacagat gaaatcaccg gcgtattagc ttgataaatc
     6181 gcccgagcaa cccgctcttc attaaacggc cataaatcct caatcgaacc acccccacga
     6241 ccgatgatca ttgtatcgaa attgcccatc tcttctactc tctggatatt cctgacaaca
     6301 ttgtccgctg cttggtttcc ttgtacaact gttgggaaca aaaccaattg gacaattgga
     6361 tatcttcttt tcaccgtcgt gatgatatcg cggataaccg ctccgcttgg actggtaagg
     6421 accgcgattc gctttgggaa acgtggcagc atttttttag gcccttgaaa aaggccttcc
     6481 ttcatcagct tttcttttcg ttcttccaat tcttgatata aagcacctat tccatcaggc
     6541 tccatgtgct ctacatagat ttgataagaa cctcccgctt catataatga aatacgccca
     6601 acgacaagga ctttcattcc ttcttttggc tggaatcgta atttttggaa tgctcctttg
     6661 aacataatag cagatatttt tgcatggtca tcttttaagc tgaaatattg atgggcattg
     6721 gcacgtaaac ggaaattcga tatttcgcct gttaaatata cgcgttctaa ataaggatcg
     6781 gcatcaaatt ttcttttcaa atacttcgtt aatgtagtga cagttaaata ttcttgtgcc
     6841 atctattcac ctgctccaaa agcttcggca gattttacgg tttgatacat cagcatcgtg
     6901 atagtcattg gccctacacc ctttggcaca ggagtgatcc agcctgcaat aggctccact
     6961 tcatcaaatt tcacatcacc gatcagctta ccgttagcat cacgattcat ccctacatct
     7021 atcactacag cacctggttt tacgaattct tttgtgacaa aatgtccacg tcctattgct
     7081 acgactaaaa tatctgcttc ttgtgccagt tcagccagat tttcggtttt agaatgagca
     7141 acagtaacag ttgcatctgc catcaagagt agctgagcca tcggtttacc gacgatattg
     7201 cttcgtccga taactactgc tcgtttaccc gccggatcga tagaataagc ttcaaacatt
     7261 ttcatgatgc cgtatggagt acaaggaatc ttgtccggtt tacccgcaag gagtctgcct
     7321 aaattcatcg gatgaaaacc gtctacgtct ttttcagggc ggatagctaa caggatcttt
     7381 tcttcatcta tatgctttgg caatggtaat tggaccaata taccatgaaa actaggatct
     7441 tgattgtatt tttcaatttc tgcaagtagt tcgtcttcag aaatcgtctc aggataacgc
     7501 tctaccaatg aatggattcc gatttctttt gctacacgtt ctttatttct tacatagatt
     7561 tggctggctg gattttcccc taccaataaa acgactaatc cgggatggat accattatct
     7621 ttcaattttt ggattttttc agcaatctct gcctgcattt tttcagctaa ttcttttccg
     7681 ttaatcaatt ctgccatcta accactccca gatatttttt gtgttttcat tctaccatag
     7741 aattttcccg atttataaca ggtttatgat gattcataga aaaaagctag gacttacgcc
     7801 ccagcttcta attctttcaa tacattggat aaaacaccgt tgacgaattt acgtgaacgg
     7861 tcatcactaa atgtttttgc caactcgatt gcttcattta aagcaacagc cgctggtaca
     7921 tctgtcacat aagtcatttc aaaaatcgcg atacgcaaga tcaccaaatc catttttgag
     7981 atccgattga tgtgccaatt gttttttaaa tgtttttcaa tcactttatc caattcatct
     8041 ttttttgcac agacaccgcc aactagcgta tcaagataaa caggaacaaa ctctgattca
     8101 tcttcgttga tcatgtcgcg gtggtctaat tcgattgcgt tgaaaatcgc atcttctttt
     8161 gttaggtctg cattgaagtc taatggaaat aaagcctgca atgccatttc ccgaatctcg
     8221 tgtcttgata attctttatt cattctcttc ctcttcgtct tcaaaaagtt cttcgattgt
     8281 tggttgttct aatttttcag gaacaacagc tactacatgg atattgactt cagataaatc
     8341 gatatccgtc atgaaaagga cttgctggct gacccgttcc tgcatttcca ttgctacttt
     8401 aggaactgaa acaccatatt ctagataaca gtaaagatct actttcaagc catcttcctc
     8461 gtttgctaag taaacacctt ttccgtgagc tgcacgtcct aataattcag tgacattgct
     8521 ggcaaaagtt ccgcgcattc cgtatacgcc ttctacttta gaagctgcaa tcccgataat
     8581 tacttcgatg acttcaggag cgatcacgat ctctcctaaa tcttggtttg cgtttaaaat
     8641 caaatttttt tcatctgcca tttatttatt cctccctttg cctaagcacg agatacatcc
     8701 gaattctcta tgatgcggca tagtgatcac gtggtaagaa aactgccgcc gatcagctgc
     8761 aaactgctat gaattggaga aaacacccaa ctttattatc tcttacagct taaaagttct
     8821 tctatagtgt atcattaaat cgagtgattt tctataacaa agtctgtgaa cactgatttt
     8881 tacgcttact tcttctattg atgaaactat aagatgatca attcttttgg tgaacgagtc
     8941 aacacacgat ttccatcttc tgtgatcaac aagtcatcct caatacgtac tccaccgatt
     9001 cctggtaagt agatccctgg ttcatcagtg atcacatttc ctggtacaaa ttgcttttct
     9061 gcacgagaag aaacgtttgg tccttcgtgg atctctaaac cgatcccatg tcctgtagaa
     9121 tgtccaaatg cttccccgta accatgtttt gaaatataat ctctagcaac tgcatctaat
     9181 tggacaccag tcattcctgg ttttgcagca tcaataactg ctaattgagc atctaagact
     9241 atttgataaa tttcttttaa tttttcccca ggatcaccaa tagcgaatgt acgtgtcata
     9301 tcggaaacgt acccttcgta atagcaacca aagtccaatg tgatcaagtc accttgttcg
     9361 atgatttttt tgcttgctac accatgcggc atagccgaac gcaatccgct agcaacgatc
     9421 gtatcaaaag aaacacctga agcgccaaga gaacgcatat aaaagtctag ttgatttgcg
     9481 acttcaatct cagtcatgcc cggttggatc attttcagga tgtgatcata tgccatatcc
     9541 gcaatatgac atgctttttc gataatagcg atttcttctt catctttcac ttctctgagt
     9601 tcttcgatca tctgtgaaac aggtactaat tccgcatcga tgatttcttc taaaaccgaa
     9661 tattctaaaa atgagactgt tgtttcctca aaagctaatg agtgcagttc ttctttgcgt
     9721 actaagtttg ctacctcatc aaaaataggt ccgacatttt taataatttc aaacccttga
     9781 gcctgcgctg ccgcctgctc ggtataacga aaatcagtaa taaaaaatgc tttttccaaa
     9841 gtaataacag ctaatcctgt cgtacctgta aagttcgtta agtaacgaag attgtacgga
     9901 ctggtaacca aaaatgaatc caaattttct tcttgcattt ttttgcgcaa tttttctaca
     9961 cgtagcatca taatgaaact ccctctttct ttgcacgctt ttttccatta tagcaaactt
    10021 ttcttcgtac acgacagtta aataaaaatt tccggaaaac ttctt
//
LOCUS       DAHEYX010000102         9948 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHEYX010000102 DAHEYX010000000
VERSION     DAHEYX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.103"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            266..472
                     /locus_tag="MB219_001705"
     CDS             266..472
                     /locus_tag="MB219_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCC8427421.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(674..1675)
                     /locus_tag="MB219_001706"
     CDS             complement(674..1675)
                     /locus_tag="MB219_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8427422.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1680..3593)
                     /locus_tag="MB219_001707"
     CDS             complement(1680..3593)
                     /locus_tag="MB219_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCC8427423.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3761..4267)
                     /locus_tag="MB219_001708"
     CDS             complement(3761..4267)
                     /locus_tag="MB219_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCC8427424.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4427..4867
                     /locus_tag="MB219_001709"
     CDS             4427..4867
                     /locus_tag="MB219_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCC8427425.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4893..6050)
                     /locus_tag="MB219_001710"
     CDS             complement(4893..6050)
                     /locus_tag="MB219_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427426.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6053..6409)
                     /locus_tag="MB219_001711"
     CDS             complement(6053..6409)
                     /locus_tag="MB219_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427427.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6707..7681)
                     /gene="sgrA"
                     /locus_tag="MB219_001712"
     CDS             complement(6707..7681)
                     /gene="sgrA"
                     /locus_tag="MB219_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCC8427428.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7877..8716)
                     /locus_tag="MB219_001713"
     CDS             complement(7877..8716)
                     /locus_tag="MB219_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCC8427429.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8901..9788
                     /locus_tag="MB219_001714"
     CDS             8901..9788
                     /locus_tag="MB219_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCC8427430.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         2991 a         1990 c         1500 g         3467 t
ORIGIN      
        1 tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat cttttctatt
       61 atattcccat attttctatt attggtctag acgtatgatg gtaaactttg tacctatctg
      121 attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg ctagatgatc
      181 attttattca catcctaaca aacttgtcac atttatagat tcttaatcta tttatcatac
      241 aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc tttttttcgc
      301 tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca ataacaacgg
      361 aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc ttcttgcctc
      421 cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct gacttataaa
      481 atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc gaataaaagg
      541 tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa ccatgaaaag
      601 cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac aacctctttt
      661 gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt tcttgcgctt
      721 gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc tccgcataat
      781 ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct tccatttgtt
      841 tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa cgatccgcat
      901 gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta ttatcaaagt
      961 aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact tgagtaagtg
     1021 cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg ttctccaaag
     1081 tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac aaatattctc
     1141 cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa tctgcttgat
     1201 cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact ggtgtaggat
     1261 gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc gtgtctgaac
     1321 atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat gcgcctccat
     1381 gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag atatccatat
     1441 tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt tgttcgattt
     1501 tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg tcaaattgtt
     1561 ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat acagtgacta
     1621 aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat ttcattcatt
     1681 taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa aaatccacct
     1741 gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct tttttttgca
     1801 tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc acttgattga
     1861 tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc ttgctttttt
     1921 ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt ctcattaaaa
     1981 atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt cttttgctct
     2041 gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc tttgagtaga
     2101 cttttattgt tttcatccac atactttacg tttatctgat catggaacac tactttcaaa
     2161 aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt tccatgccct
     2221 gctccttcta ctaataaaag atctttatac cctcctgata actcatataa tttttgcgcg
     2281 ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg caatgaatct
     2341 gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg atcaaaatag
     2401 tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat gatgtctaac
     2461 tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt aaaacgtagt
     2521 tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt gacaggaata
     2581 tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata aagtaaaatc
     2641 ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc atctgactca
     2701 tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga atcaacaaat
     2761 aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat cccgttattc
     2821 caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg atcgacataa
     2881 tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg atgaagttgt
     2941 tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc tgtacctcca
     3001 agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag ataagtagca
     3061 atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag ctcagtgact
     3121 aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat ttctgtcatc
     3181 tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt accgatatca
     3241 tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc gtcgtttgac
     3301 atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt cgtttcttga
     3361 tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt ttgtgaagta
     3421 ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc ccgagattcc
     3481 gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt acttacagca
     3541 aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt cactcctttt
     3601 tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa ataaatggtt
     3661 tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc agaatggata
     3721 aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc aaaaatgtca
     3781 gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct ttcataaaag
     3841 gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct aatccatgag
     3901 caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat tcgatcgaat
     3961 gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa ttcgcatggg
     4021 tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc tcccatggtt
     4081 ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt ttcccaatcg
     4141 tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat tcgtgtatcg
     4201 gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg tcggctgtgt
     4261 ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt atctagctag
     4321 ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact tttcgactat
     4381 ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga tgattaaatt
     4441 gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta tcgtccatga
     4501 tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg aatttatcca
     4561 cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt ctattagtta
     4621 taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata ttgagattcg
     4681 tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa aacttttgac
     4741 tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa agcaagagct
     4801 caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc tttttgcaat
     4861 gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa tttttctaat
     4921 atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg cgcccaatca
     4981 ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc atatgccttt
     5041 ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga tgcactatat
     5101 tttattgggc gttcagtcat agataatatc cttttctttg cccataattc ctgtgtttta
     5161 ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa aatatccctg
     5221 actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt attaagctct
     5281 ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat cactggaaat
     5341 atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt cttttttgtc
     5401 ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata atttttttct
     5461 gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga cgattcaatc
     5521 cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc caaataggtg
     5581 ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat gtgattcatc
     5641 gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc ttcttttgtt
     5701 atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga gtggccttgt
     5761 ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc tatactacgc
     5821 gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt tatacttgat
     5881 aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa aaagtatcgg
     5941 atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga taaatgatag
     6001 tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa acttatataa
     6061 ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa tccagtaaaa
     6121 tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa aactgagaag
     6181 aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat tctggatacc
     6241 tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg gtgtagttta
     6301 gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa caatagttca
     6361 aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa gtacatactc
     6421 ctttttatct attatttaga cttattccga cagctacatg aataacgtac aaatagattc
     6481 gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta ttcttgctct
     6541 tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta aacaaaaata
     6601 gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa atattcactc
     6661 aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat ttcactcgat
     6721 ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca cccatccatg
     6781 taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt ctattatctg
     6841 tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc tgttttagca
     6901 cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg ataggtacca
     6961 aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac
     7021 tctcactact catgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7081 tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg
     7141 tgttttctgt gctactttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt
     7201 ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac
     7261 tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa
     7321 ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact
     7381 caataccagc ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac
     7441 ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt
     7501 tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag
     7561 ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa
     7621 tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca
     7681 tttctatcac tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa
     7741 tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga
     7801 atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc
     7861 ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat
     7921 atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg
     7981 cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga
     8041 aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccaat
     8101 tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc
     8161 tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca
     8221 atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc
     8281 taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa
     8341 taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat
     8401 acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga
     8461 gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc
     8521 tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga
     8581 ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat
     8641 tattgacaag aattcctaaa acaaaaccaa ctaagactat taaaacagat ccaatcaacc
     8701 gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc
     8761 ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact
     8821 aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga
     8881 ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta attatgtgcc
     8941 tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa
     9001 cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa
     9061 ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa
     9121 aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc
     9181 caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct
     9241 ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta acagataaag
     9301 atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt
     9361 cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca
     9421 agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt
     9481 ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa
     9541 acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt
     9601 atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg
     9661 agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa
     9721 tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat
     9781 tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga
     9841 aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt
     9901 atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatata
//
LOCUS       DAHEYX010000103         9873 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHEYX010000103 DAHEYX010000000
VERSION     DAHEYX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.104"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(184..1062)
                     /locus_tag="MB219_001715"
     CDS             complement(184..1062)
                     /locus_tag="MB219_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCC8427431.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1101..2219)
                     /locus_tag="MB219_001716"
     CDS             complement(1101..2219)
                     /locus_tag="MB219_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCC8427432.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2216..4315)
                     /locus_tag="MB219_001717"
     CDS             complement(2216..4315)
                     /locus_tag="MB219_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCC8427433.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4366..5187)
                     /locus_tag="MB219_001718"
     CDS             complement(4366..5187)
                     /locus_tag="MB219_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCC8427434.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5184..5993)
                     /locus_tag="MB219_001719"
     CDS             complement(5184..5993)
                     /locus_tag="MB219_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8427435.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6014..6508)
                     /locus_tag="MB219_001720"
     CDS             complement(6014..6508)
                     /locus_tag="MB219_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8427436.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6520..7008)
                     /locus_tag="MB219_001721"
     CDS             complement(6520..7008)
                     /locus_tag="MB219_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8427437.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7085..9781)
                     /locus_tag="MB219_001722"
     CDS             complement(7085..9781)
                     /locus_tag="MB219_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCC8427438.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         2978 a         2230 c         1634 g         3031 t
ORIGIN      
        1 agcgcacaac aaaaagaaaa aaggaaacgc agaagcattt cctttcagtg atttaatgaa
       61 aacaacacga tttgacttag agccttttta tttgaattca ttttcgcttt atccaacttc
      121 atttgatcat ttgattactc ggttaaatac ttttgacatg agtatcaatg aaacattcat
      181 taattatggt tgcttatttc ttaccgcttt tctggccaat gctaatccgc cgattattcc
      241 ggcattatca cctaattcgg gcgtaataat gtagctatca attggtggta tttcaacata
      301 tccatttata agctcaacaa atttatcctg aacttttttc ttcatgtgtt cttgtttcat
      361 tacaccaccg ccaaaaataa ttctatctgg cgaaaacatc agtgttgtat tgtatgcaca
      421 ttgggcaagg taaaaggctt caatctgcca aaacgaatcg tcagccaata aattctggcc
      481 ttttacaccc aaccgttttt caatcgctgg tcctgctgcc atcccctcta aacaatcttg
      541 gtggaaggga cagtttcctg aaaaattgtc ctttggatgt cgcttgatca acatatgccc
      601 catttccgga tgactgaacc cttccacgaa agtcccattg aacaaggcac cacctccgat
      661 acctgttccc acagtgaagt atgcaacacc tgctaaccct ttgccagaac cagcaacata
      721 ttcaccatag caagccgcat tgacatctgt tgtccattca atcggaatat tgaaatattg
      781 ctttagcgtt cctacaaaat tgaaattttg ccaagcaagt tttggtgttg atgtgatatg
      841 accgaaactc gttgatttgc attgaatatc aattggacca aagcagccga ccccgatgcc
      901 tgccaattgt tccttaaaag gtttaaaaaa atcaatgacc tgcttcattg tttcaactgg
      961 agttgttgtg ggaaaactga ctctttccaa aatcgttaat tcctcagtac cgactgcaca
     1021 gacaaattta gtccctccag cttcaattgc cccatatatc atcataggtt cctcccttcg
     1081 aatatgtgaa aaacacttca ttaacgcggc aagcgttctc gatcagtgaa atgacgttta
     1141 gtatttcctg aagcttctgt tgaataccag taagcgacac tagcaacatc gtcttgccgc
     1201 tcaaataagc gaacatcatc gttgccgatt tgttgtaaag tgaccgataa atcttcggta
     1261 aaacagatgg ggtcttgaag atgccaacga taaagaccat gtcgtggcat tgcgtcgtca
     1321 ccaaaggcat gaacggcatt caattttccc gtttcaaaac gatctcttgt ggcatctcta
     1381 tttgattggt aaggataccc taaaaaggct gttgaatagt ttttcgcatg tggacgtccg
     1441 aactcatcgt actcatgaaa ggcccacgcc ccgccaaaat aatcttctga acctgttgag
     1501 gttacggtcg gatactcact atccccatca atgtagaatt taaactcgcc ttcgccccac
     1561 caataacgtt ctaggcaggt aagcgcaagg taagtaccca cgtaatagcc ttgacccttt
     1621 aatcgatcaa ttaacttata atcctcctgt aatttggtaa tgcgttcccg atgccaattt
     1681 gcgtgaaaat ataggctatt ttcttccaat tcgtccacca aagcgtaatt tatcgtataa
     1741 aaaaatgagg acatatcctt cggatgtaaa ttctcgatcg taattttagc ggatgtttgg
     1801 aaaggcattt cgaaatagga attaaagccc cctgtaggat taaccactat cggcaacgag
     1861 ttcacctcgc aacgctctcc aaacccatta caaaagaaat cccctaaagg actttcaaca
     1921 gcagggctat ccgacccatc ccaataaatt cttaagatta aatcacgtaa aacaaaactg
     1981 ccaaattctg ttcgatcacg aatcgtcatc cacatatgac gaattattcc cgttccagta
     2041 atatccgcaa tcacagtttc ttcaccagaa gtcagcggaa ttgcgccacg acctttgcgg
     2101 cttggtccta gctcgcttgg caacatcgca gctttacctt tctcgcctgt tggattttcg
     2161 ggagtgattg ctcgactctg gattttttta aattgggtaa tattttttag tatactcatc
     2221 tttttctcct ttgaatgtga atcgtttttc ccttttctac gtgaagaatt tttttcgtat
     2281 cacttatttt caatggcttt tctgacaaat ttgaaatact caactcggat tgcgtacatt
     2341 caatcgaaat gaaggcttga ccgaacttca aattcttcca agaaaacgtt aaattatcaa
     2401 caggatcaat ttgcaatact ccttttttga atttcaatcc gaaatattga ctgatcatta
     2461 ggctgacaaa aagacccgat gcccagccgc atttgccgca gccaaagtct cgcacgactt
     2521 ctccttgttg agcctctaag cgatacggcc accaccacca cgaaccgtct aaatctgcca
     2581 aacaaattag ttcttctaat cgggttacga aagcttgagg gttgtgaagc tcgctcatta
     2641 aaacagtgat aaagccagga aaagtggcgc cggattctaa tccccaagtg atgcctttga
     2701 tttcctcgct ataggttgga ttgaaagaac tagcagaaaa ggccatggtg ttctgatata
     2761 aatgatcttt attatctaaa aattgataaa aaggcatcaa cgtagtgtct gattcttctc
     2821 catcatgcat caataaatta atttttccat ccacaatcac atcacttaaa aagattaacc
     2881 cttgctcaag aatcggtttt tgatagtgct tcactgaata ggttgttttc tcctcatcac
     2941 cgattccctc caaaaattgc tcaccaaaag gaccatcgat agtcatttgt ttacaaattg
     3001 attcctttag tttacaggca atgtttttgt atcgcttgct aagactcagt tgttctaggg
     3061 cttcaaaaat ttctgcaagt ccaatacagg ccttccaaag acaaagattg ctgccggtat
     3121 gatactttcc aagtgcaaag gcgtctgata accaaacaga tggaaaaagc attggttccc
     3181 ctgaccgacg ttgagctaat atgtcctcaa taattcttga agcattcagt agaacctgcg
     3241 gacaccgctc aaagaatttt gtatccccag tatgttcaaa ataaagacta gccagtaacg
     3301 cactggataa cgaattactc agagaatgat tgatacctcc tgtaaatttt gaaccaggaa
     3361 acttaacgcc atattgatca aaccatagaa ccgttttttg acataactcc ggttcaaaca
     3421 tggtgaatgg caatgatgcg taatacatat ccttattcca aattcgatta gtagcaggat
     3481 agctgcccca attgctgccg acgacctgtc cctgtcgatt cataccgaaa ctagaaaagc
     3541 tttgataaag tgcacgacga tagagatgga caactctatc gtttggcatt gacagctgcc
     3601 caaatagctg agcaaaatag ttcaatgttt ccttcagcca tttttcgata tcagattcct
     3661 gaaaagtcat tacttcttca aaagcatttg ggtccacaaa agcaatggcg aagcttgcca
     3721 cttcctgttg tgcgatcgtg tccttatttc ctttttgaga aatcaacaca ttttgctgat
     3781 ccgaatactt ctcttgatac gaaggcacat ctttcactga aacttgaagg ctttcgtgtg
     3841 tttcattcac aatgtaaacc tgttgaacaa gcatacttaa acgcttatta gccagtatgg
     3901 gagcaaagga tactacaatg accttaaacg tcgaataatg atgaatatat ctaggcaaac
     3961 agtcttgaat cacatcgact tcaatacgat gatccgattc tgacaaatgg tagacaatcc
     4021 cgtttacttc aacctttaag gataacgaat tggtttgaga gagctgttta ttgacccaaa
     4081 cacctggttt ttcctcagtt agtaaatttt ctcgataaaa ggtcaacgtt tgaatcgtcc
     4141 cgcaaacatc aaggtttgct aatgtctttt gattacttac atcgaaggtc aatcccttta
     4201 catcaggaat attccgatag tagctgtatt cgtttgaagt agtcacattt ccgtcgttta
     4261 actcagcatt aaaaaatggg tgtttgggtg aataaattac ttcattaata gtcatctttt
     4321 ccctccttgc taaaaaaact gcagcctagt agctgcagct tcctactatg cgattccaag
     4381 taatgacaat aaaattccca agacgaccat tccaaaaata attttgatga cgccgacttt
     4441 tttatctagt aatttgaaac agctcaatgt taccaataac ggtacaattc ctttaaaaat
     4501 actatcgagg atttcttgga cattcattag ttcttcgccc ttcattgaaa agctcaaaac
     4561 ggtattgaat ttgaccatac tgatcgtcat tgcacctacc atgatcagac ccaagatact
     4621 agcagctttt gtcagaattt caattaaccc gttcgcatac agcttttcaa tatatttaga
     4681 gcctaatgaa tagcctaaaa cgccgccata gtatcgcatc aagtaattag gtatattgaa
     4741 cataactaaa aagataattg gtgccaaaat atttccggca ttggctaagt tgatcgctag
     4801 tcccgcggta acgacacgcc aaactcccca gaacaatgaa tcgccaattc cagccaatgg
     4861 tcccatcaaa ctcgttttga tcgcgttgat actttccacg tcaaaaccag ctttttcaga
     4921 attttccttc tccatcgatg ccgcaatccc catgacaaag gacgcgcaag gaatggtcgt
     4981 cgaaaaatac tgtgtatgac gttccagcgc cgcagctcga tcttcttcac ttttgtagaa
     5041 atgattaatg aagggcatca tagaataggc gaaaccaccg ccgcccatcg tcaccggatt
     5101 caccgaagca tacatagtca tcgagcgcca aaacatcttc cgaaccaatt ttttctcatc
     5161 tttagaaatt ccatcagcta atttcattga aagaaatcct cctcttcttg gtcaagcgtt
     5221 gcatttacag cagcatcgct ttgaactgca tgaacagata tattttttag ttgtagatct
     5281 ctttgggcta taacaattgc aataatcgtt ccaactgcgg caattgcaat caatggtaac
     5341 tcgagatacg ccataacaac ggccccaaag aaaaaccaca cagcaatatt tttactccaa
     5401 agcatccgta acagcatcgc catcccaact gccggcagca gatttccagc gactgttaat
     5461 ccgttcatga tgacctctgg aatattatcc aagacgcttt tgacaacatc tgctccaaat
     5521 aaaacggcaa agaatggaat aagagcataa agcgcatagt ggacaaacca cataaagaag
     5581 tgcagattga tgattccttt ttgatcacca gattttgcaa atttgaaaaa tttagcagcc
     5641 atcggtccac atacaaaaga aaaaatcata ttcattaact gtaatcctaa tactgctagt
     5701 ggaaagacaa tgggaataat cgcatccacg tttcctccca ttttgattgc gaacgctgta
     5761 gccagcccag cagccaaaga aggctccgcc gtactagaga ttccgatatt gacgacaccc
     5821 ataaagatgg cttctagtga cgccccgaca atcaaccccg tctgcaaatc ccccatcaac
     5881 gctcctgcta acggagcaac aacaatcgga cgactcagca tggacatccc gaacatttca
     5941 tgtccagcaa ctccaataaa aacggccaaa gcgactacaa atgcatcata cataattttt
     6001 ctccttcctt tttctaaaaa tcactcaaaa tagagatagc tgaacgtttt tccatcgccg
     6061 gtgtaggctg aaaaacggta tctggataaa gttcaactaa ttcttttaag tcttgaattt
     6121 ctgacgtcgt taacatcact tcctttgata aggttttctt atcttccgat ggcggtgtca
     6181 ttttcccagc attccccacg ttcattaatt cgatttccgt tgggaaagct ttcgcaactt
     6241 ttaccgcatc tttgacatta gggactaaaa ccatcaagcg catgtccttc gcccgcggat
     6301 cttttaacaa atgaatcgcg tcatcaactg ttttaataat gactttgaca ttcgatggtg
     6361 cagccatctt caatgtcatc ttttgaatat cgttggtagc cgcctcatcg cttgctacta
     6421 ctaacccggt taaatttaga ctctttgtcc aagtcatcgc tacttgtcca tgaattaatc
     6481 gttcatcaat tcgcactgct ttaatcattt ttcttcctcc taaaaaaaat cttcctcttc
     6541 atttactaca tgtacctcat caagaatggt catctttaat tcctgttggc atttatttac
     6601 cagttcctga atatcctcct ttgttaattc cttggttgcc aatactattt cgagcagaat
     6661 tggcagatta aagccagaca aaatagttac agcaatctct ttctccaaaa aggttttggt
     6721 aatcttctga ttgacacttc cgccaaataa atcggtaaaa acaaaaatct gatcctgctc
     6781 gttcacctgg tttacaaata cattcaactc ctgttcaaaa tcacactcat ccacataagc
     6841 attaatcacc gtaatctctt gaaccttccc cgtcagtaat tgaatactgc taagaaagcc
     6901 tgaagctaag tgaccgtgag tcgctattag aattttcttc atatggttaa ttcctttctt
     6961 tctgttcact tttatataag caattcctgt gccaactttt aaaaacattg gtttaatcac
     7021 gttaattcac attaaaaaaa gtgtctaacc taaaaaaaac agattagaca cttttttaga
     7081 cactttattt ttgaatatac ggtcctaata attcatagag cagcgaaagc tcataagcat
     7141 tgactttaaa attatatttc atctccattg attgaaaaat acttctagaa acataataaa
     7201 attcatcttc atctggtttc aaatcataat tcggttcttc agatttttct gtccgagcca
     7261 agaataaacg ttccatcatc aatgccgtat gcatatagag atttagtttt acacggccat
     7321 ttaaagtgaa atgatagtaa ttttcatatt tagaaatgac tgtttccact tcactcatca
     7381 cgatcgttgg gttcagaaag ttcaaacggt ccgcaattcc ttccaacgaa aagaatttta
     7441 ctagctcctg aatcaagcgg ttaaattctg ccggcttcat ttcaggactc aaaatatgct
     7501 ccaaattggc tgctccatca ccgtctaaga tgtcataaac attaatatta ggaattgaaa
     7561 aggaacgtgg aagatccgaa gtcgtgatga ccaattttgt cttgtcaaaa taagtttcat
     7621 cattttggtc gattaattca cgtagttctc tgtagtcctt cgtgaaaact tcgatggatt
     7681 catccgataa atgatgacga aaaacatctt tcaatttgtc cgaaatcccc aaacccgaca
     7741 tacaggaaat gataatgttg ctactttgag aaaagccttc aaagtattga acattgattg
     7801 aatacttgtc tgctgcatcc tccgcaatct gtttaaatgg cgcattcatc gtcatcttca
     7861 agccaatatc aagtgcaatg gaagtcgtca aattattcag aagaagtaac tcccctttta
     7921 aatcgttctt gatctgtgtg taaatttgat ttaaagagcc catatctacg atcagcacta
     7981 gactatctgt attcacttgc cgtttcacga aattccttgt cgattcgaca atctctgaca
     8041 cattactctc tactggcata tccaacgctt caaaaacata attgccgcat aattgattca
     8101 ctactgcttg aatgctgcta gccgtactat ctccgtgagc aagcaacagg cccttcagct
     8161 cgatggattc atctacataa tcagataaag tcacgacaat aagcagtctt agaaagttct
     8221 cgtccttttc tgagagcggt ggtaaatgct ccaagaactg attacataag taagtcgtcc
     8281 gaggtagatt tgcagctaat gtacgattta tcccggttaa gtctgtctta tagcttgggg
     8341 aaatgacata ggatctgcta ataatttctg caacaggtcc actcgtcgtc aatccgtacg
     8401 tttttcctaa aacggatacc cagacctttc taaacatctg aatcatcaat tttctactca
     8461 ttgataaaaa agaatcctta tcaatgtgat gcaccaaatg ctgtacttca ttctttatcg
     8521 aagaaaaatt ttcattgaaa ttaccttcat ttatcagctg agtaattttt tccaaaatcg
     8581 aatcataatc aaacggtttg attcgatccg tttttatctt ttgatttttc tttatcaata
     8641 aactagcaat cggcaagctt tctacttcgg attccagcat atttaactga atcaagatct
     8701 catttttccc aatcgttaat ggttcttctt gtgttcgact atacacatcc gcacaactga
     8761 ttttaatgat attcttcagc ttaccaatat tcccactaaa gggatgagat aacattaaag
     8821 acaaggcttc tcttgaaaca ataatatctt tttgtaaaac atccgcttca ttttgaaaaa
     8881 ataattgaat cagctctaat cgttctcgaa tggggcgatc ttcaaaagtt ggcaacataa
     8941 cactaatttg aatacgccga tcaaaggtgt ctaataaata attttcacct ttttccgtcg
     9001 tggcgaaaat aaaacgaacc ttagcagaat gccaatttac attttcaccg actggacgat
     9061 agtttccagt atcaataaat agaaagagct tttcttgatt ttctttcgat aggcgatgga
     9121 cctcatcgag aaaaagatag ccgttgttgg ccgttgccaa taaaccatcg gtatcctttt
     9181 cagcccccgt aaaggccccc ttcttgtatc caaacagtgt tgccgaaagc agttcgggat
     9241 tgtccgcata atcggcacag tttaagatag caaatggtgc atctttttca ataatttgct
     9301 catggatagc gtattcaaaa atttttgtgg ctaaaaaact ttttccaaca cccgtggctc
     9361 catttattaa tacactgagt ccgttaggcg gatatttgac agcggctata catttttgaa
     9421 tgacctctct cagacttccg tcatgaccaa ctacagaatg aaaggcttcg tttatttgct
     9481 ccgtattatc tacaaccgca tattcatcta aaggcttcca caaaacaggc tttcccggta
     9541 atttctcgac ctttttatct tccactaatc tcgtcaaata atggctggca ttctgacgag
     9601 agatacctag attcttcgaa attttctcgg cagtgacaga agacatattc cctgttagtc
     9661 gtatcagctc atcaaaaact cgatctttag cgctcttttt cattttttcc tccagtttaa
     9721 tgtgtctaaa agcataataa actatcggct gcaatatgtc tattctatat ttgtattcca
     9781 atttttactt tgaaacacaa atagtcgaat atttgcctca gatatctgac aaaataattt
     9841 atctaccaat ttagttttta tctaatatga ttt
//
LOCUS       DAHEYX010000104         9807 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHEYX010000104 DAHEYX010000000
VERSION     DAHEYX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.105"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(28..2148)
                     /locus_tag="MB219_001723"
     CDS             complement(28..2148)
                     /locus_tag="MB219_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCC8427439.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2378..3556
                     /locus_tag="MB219_001724"
     CDS             2378..3556
                     /locus_tag="MB219_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCC8427440.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3572..4330
                     /gene="aroD"
                     /locus_tag="MB219_001725"
     CDS             3572..4330
                     /gene="aroD"
                     /locus_tag="MB219_001725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCC8427441.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVKE
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4353..4838
                     /gene="ybaK"
                     /locus_tag="MB219_001726"
     CDS             4353..4838
                     /gene="ybaK"
                     /locus_tag="MB219_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCC8427442.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4909..6162)
                     /locus_tag="MB219_001727"
     CDS             complement(4909..6162)
                     /locus_tag="MB219_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCC8427443.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6279..7628
                     /gene="gdhA"
                     /locus_tag="MB219_001728"
     CDS             6279..7628
                     /gene="gdhA"
                     /locus_tag="MB219_001728"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCC8427444.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7740..9086
                     /locus_tag="MB219_001729"
     CDS             7740..9086
                     /locus_tag="MB219_001729"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCC8427445.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9394..9780)
                     /locus_tag="MB219_001730"
     CDS             complement(9394..9780)
                     /locus_tag="MB219_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCC8427446.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2010 g         2887 t
ORIGIN      
        1 gtcgctgctc cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag
       61 ttcttggtac gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa
      121 gacactagtt gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt
      181 cttgtagtca taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag
      241 aagatcacca ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag
      301 tgaatcaatt tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt
      361 actatagatc gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa
      421 agaaacaagc tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt
      481 tgtatctaca cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt
      541 gtgattgatt ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat
      601 ttgtgcattg tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt
      661 tttatttctt gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc
      721 atacaagccg ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt
      781 ggctgtcgca aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc
      841 ttcgtcattg gtgaattttg aataaggata atggttagat acagcaatga attttgaata
      901 aaatggttgt tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg
      961 caacccgtat tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata
     1021 atcatatcct aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg
     1081 gaataccgca ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt
     1141 attttttccg cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc
     1201 catcagcgtt tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa
     1261 actgaacgtg ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag
     1321 accattctca tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat
     1381 ataaatcaca ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg
     1441 ttcttttaca taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg
     1501 gtttgtctgg taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt
     1561 gaccaaataa tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat
     1621 aaaaagattt cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc
     1681 tcggatcggg cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat
     1741 aaaatcgatc caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag
     1801 accactcgct actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc
     1861 tcgataataa gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat
     1921 cgtgatataa gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt
     1981 agttgcaata gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg
     2041 gaaatcaact aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa
     2101 aaatccgagc ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct
     2161 ttctgcactt cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc
     2221 atttatttta ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca
     2281 ttaaggtttt ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg
     2341 ttatacttgg gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag
     2401 agcaagcaat cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc
     2461 ccaaaaagaa accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa
     2521 aggatatcta ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc
     2581 atttgatcaa tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt
     2641 tttttcagat gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg
     2701 cggtctgatt gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt
     2761 atatgcacat cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg
     2821 aggctatgag aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg
     2881 aaaagaggca ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt
     2941 aaatgaagga ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc
     3001 agaaggtttc gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc
     3061 tgttgctgcc gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag
     3121 tttaaaaaaa acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat
     3181 cattgttatg gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga
     3241 catgattgta ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc
     3301 aaaagattat ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact
     3361 tattgcgtct acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga
     3421 ggaattccat gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga
     3481 tttccagacg acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat
     3541 taaaaataac cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg
     3601 aagcggtaga ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg
     3661 tgaagcagtt cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta
     3721 tcgtgaagtt ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct
     3781 tgatggactg ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat
     3841 atcgatccgt cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat
     3901 ggtcgatatc gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa
     3961 gtctttaaac attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc
     4021 tgtcttgctt taccgattga atgttaagga acattttgga gccgatatcg gaaaactggc
     4081 agtaactccc aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa
     4141 tgcttttgtc tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg
     4201 tctggttggc ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac
     4261 ttctgctcca ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga
     4321 accgttgtag aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga
     4381 atgcaatccg tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg
     4441 acgaaagcca cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta
     4501 tatttaaaac actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg
     4561 gagaagccga attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa
     4621 tgctccattt aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta
     4681 taggtatgaa aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa
     4741 tcattgtctc ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg
     4801 cattaacgag cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa
     4861 ggaggccggg atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg
     4921 cccggcattc attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc
     4981 agtataacca ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact
     5041 ctccaatgtt ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt
     5101 ctcaatagga aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc
     5161 ttgaatttgt ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag
     5221 aaagaaaact aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt
     5281 ggttgcttta cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa
     5341 acaatccaga gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact
     5401 tgaacaacgc agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt
     5461 atgttcagtc aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga
     5521 aagcccttga agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt
     5581 cgcataaaga ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt
     5641 agaaccagta gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa
     5701 ctcttcgaat tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac
     5761 tcgagtagag aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat
     5821 aatcgttttt gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg
     5881 tacgccttgt gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact
     5941 atataaaaac tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt
     6001 cgtaacattg caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt
     6061 ttcgatcaag agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct
     6121 tgttccagcg ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa
     6181 gtgtagcata tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga
     6241 gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg
     6301 ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag
     6361 caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg
     6421 aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt
     6481 ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag
     6541 ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga
     6601 aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag
     6661 gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt
     6721 gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg
     6781 gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga
     6841 aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac
     6901 gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa
     6961 aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg
     7021 caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt
     7081 acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata
     7141 gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat
     7201 ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa
     7261 tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg
     7321 ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt
     7381 gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga
     7441 acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg
     7501 aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc
     7561 ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt
     7621 tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa
     7681 aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa
     7741 tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag
     7801 ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag
     7861 gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac
     7921 gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta
     7981 tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca
     8041 atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca
     8101 gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg
     8161 ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc
     8221 ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata
     8281 aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc
     8341 ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg
     8401 ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact
     8461 caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt
     8521 accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg caatacttct
     8581 cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc
     8641 cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc caagaaggac
     8701 gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc
     8761 ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg
     8821 taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact
     8881 tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg
     8941 aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg
     9001 tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg
     9061 ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt
     9121 gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact
     9181 gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta
     9241 tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc
     9301 tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc
     9361 acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac
     9421 agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc
     9481 tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt caccagaagt
     9541 aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta
     9601 tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat
     9661 tggagctttg actaccgcat aataatcttt acctacataa gtagactgat agtagtccca
     9721 acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat
     9781 gaaagatctc cttttttatt tttatta
//
LOCUS       DAHEYX010000105         9797 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHEYX010000105 DAHEYX010000000
VERSION     DAHEYX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.106"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            175..1173
                     /locus_tag="MB219_001731"
     CDS             175..1173
                     /locus_tag="MB219_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCC8427447.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1285..1794
                     /locus_tag="MB219_001732"
     CDS             1285..1794
                     /locus_tag="MB219_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCC8427448.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1824..2483)
                     /locus_tag="MB219_001733"
     CDS             complement(1824..2483)
                     /locus_tag="MB219_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCC8427449.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2485..3249)
                     /locus_tag="MB219_001734"
     CDS             complement(2485..3249)
                     /locus_tag="MB219_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCC8427450.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3257..3934)
                     /locus_tag="MB219_001735"
     CDS             complement(3257..3934)
                     /locus_tag="MB219_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCC8427451.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3971..5356)
                     /locus_tag="MB219_001736"
     CDS             complement(3971..5356)
                     /locus_tag="MB219_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCC8427452.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5566..5650)
                     /gene="ffs"
                     /locus_tag="MB219_001737"
     ncRNA           complement(5566..5650)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MB219_001737"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5673..6188)
                     /gene="tadA"
                     /locus_tag="MB219_001738"
     CDS             complement(5673..6188)
                     /gene="tadA"
                     /locus_tag="MB219_001738"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCC8427453.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6295..7152
                     /locus_tag="MB219_001739"
     CDS             6295..7152
                     /locus_tag="MB219_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427454.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7233..7568)
                     /locus_tag="MB219_001740"
     CDS             complement(7233..7568)
                     /locus_tag="MB219_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427455.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7635..9005)
                     /locus_tag="MB219_001741"
     CDS             complement(7635..9005)
                     /locus_tag="MB219_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCC8427456.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9083..9619)
                     /locus_tag="MB219_001742"
     CDS             complement(9083..9619)
                     /locus_tag="MB219_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8427457.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2898 a         2014 c         1628 g         3257 t
ORIGIN      
        1 ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt tgttttcttt
       61 tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat tcaaacattt
      121 ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat ttttatggaa
      181 atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc tttttacgca
      241 ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg tggtcaatta
      301 gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc aataaaagca
      361 agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac attccattta
      421 aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac tacaaataaa
      481 ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt ccagccaaga
      541 aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta tttcgtgaat
      601 gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga ttctgctatc
      661 gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca tcgtcgcccg
      721 gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt caacttgctt
      781 actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga tatccggtta
      841 aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat cgtcaattat
      901 ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac cagaaatgcc
      961 attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc tggtgacatc
     1021 tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc acctactgca
     1081 gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg tcacagcaca
     1141 agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg catttcaagt
     1201 taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa atagagaaag
     1261 aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga agaaaaggca
     1321 agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact tgtcatgttt
     1381 ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag tgtcaacagc
     1441 gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt agatatatta
     1501 gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga cgaaggcctt
     1561 tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac gatcggcttt
     1621 acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact aaacagccac
     1681 gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc cgctgctgct
     1741 gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca ataattcaac
     1801 aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg tttcaagcta
     1861 ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa aaataagata
     1921 aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt aacgggatcg
     1981 aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa gcgatcaaat
     2041 cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg aaacggacga
     2101 atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat actcgggatc
     2161 aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc ataaaagtga
     2221 tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt gaagggatta
     2281 ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc taaagaagta
     2341 taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa gttgatcgtc
     2401 acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc atattccatc
     2461 cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt gtttcacata
     2521 ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg ataaaacgag
     2581 caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt tatctccaac
     2641 catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca taatgatggc
     2701 ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa aagagatgac
     2761 tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg ggttcgttcc
     2821 aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt cataggtcag
     2881 ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg taaatccggc
     2941 agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt tcccttttcc
     3001 atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct ctggagctac
     3061 atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact tagtcgtgtt
     3121 attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa atcgtttacc
     3181 ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta aatatccttt
     3241 atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt tttgtttttt
     3301 ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct gactctgttt
     3361 ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt ttctcatcga
     3421 tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt tcttttttac
     3481 cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg taaagatagt
     3541 cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct tggaaaaatc
     3601 cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat cgggctagta
     3661 catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa tcggacacaa
     3721 gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc actagctcgc
     3781 ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct gtcagttctt
     3841 cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct gttttatgct
     3901 caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc ttttttatta
     3961 taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc acacttcgga
     4021 taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga aaaaatggaa
     4081 caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg attggcttgt
     4141 tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag accatctgcc
     4201 caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa aagttacgat
     4261 ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg acatttaaca
     4321 agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc ccttttggaa
     4381 tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct cgttctttca
     4441 ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga ctcagagaaa
     4501 atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct agataactat
     4561 ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg gctttggctg
     4621 aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg acatactgcc
     4681 cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct ctgaataaat
     4741 gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca atttggaaat
     4801 catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa cgtaaatctt
     4861 cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc ggattataag
     4921 taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt ttcgttgtaa
     4981 tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg aataagttat
     5041 ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct ttggaatttc
     5101 ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc atcagcaaga
     5161 tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca aaatcaccta
     5221 gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta cgttgatcca
     5281 aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc gtatgtaaaa
     5341 tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc ataggcagct
     5401 ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag caaattgtat
     5461 aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat ttttacagta
     5521 ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata atcagacaca
     5581 tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca agcccaccat
     5641 tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga ccagtctttt
     5701 tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat catcccacat
     5761 tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc gtcctccaaa
     5821 agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa ataaacttct
     5881 gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa ggtaacaaaa
     5941 agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt gattgcaagc
     6001 atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc tcttccaatc
     6061 acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat ttctgctttc
     6121 tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc aagatttgta
     6181 tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca tatgataaaa
     6241 aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa caagatgaaa
     6301 atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc agcatcagaa
     6361 gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca agattcatta
     6421 tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga tcaaaaaaaa
     6481 cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa aagctttcgt
     6541 ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg gcaagatact
     6601 attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac agatgcgctc
     6661 ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg tatttttcta
     6721 tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt ccattcacct
     6781 ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac agagcaaaac
     6841 aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca ttactataca
     6901 aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat caaacaaaat
     6961 gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag ctctgctgca
     7021 aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt tcaagaacag
     7081 tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt attgcttcat
     7141 gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca accccttatt
     7201 tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac aaaatgcatt
     7261 agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac agagattttc
     7321 ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc actatctgaa
     7381 agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata gacgaatttt
     7441 tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc cgatacaacg
     7501 actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac ttttgttgat
     7561 gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac aaaaaaataa
     7621 acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt tactgttcct
     7681 tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac ccatccttga
     7741 ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt ttgagtgatc
     7801 ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata gctaggttca
     7861 aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt taaaccatcg
     7921 agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa aagtcttcca
     7981 gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg ctcttttgtc
     8041 atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt ttgtccatga
     8101 aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca tatacgtatt
     8161 gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac ggattgagtc
     8221 aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat gatctcttca
     8281 ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt ttttagcatt
     8341 tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg agattcttct
     8401 gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga acaaggagca
     8461 tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc gggtgcatga
     8521 tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa aatcttcgca
     8581 cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg agcagctaat
     8641 tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc gggttttgct
     8701 gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata cccagcttga
     8761 tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta gggagcgggt
     8821 tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt tagtggattc
     8881 caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc ggatgcttct
     8941 tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt cgcttcctcc
     9001 ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata aaggtggctg
     9061 atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt tccaagtatt
     9121 catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa tcttgagaag
     9181 aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc gacttttctt
     9241 ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat agcgaatcca
     9301 tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa aaaaaatctt
     9361 tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag tagttttcaa
     9421 aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat ttgaaatgtg
     9481 tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca gtaattgact
     9541 tcattccata ttcttccacg tataccatac ttttgaacag caaatattct ttactataat
     9601 ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata aataaataac
     9661 ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta tatccattaa
     9721 aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta ggaaataaac
     9781 aatagaataa acaaggt
//
LOCUS       DAHEYX010000106         9791 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHEYX010000106 DAHEYX010000000
VERSION     DAHEYX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.107"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            291..1430
                     /locus_tag="MB219_001743"
     CDS             291..1430
                     /locus_tag="MB219_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCC8427458.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="MB219_001744"
     CDS             complement(1497..2072)
                     /locus_tag="MB219_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCC8427459.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="MB219_001745"
     CDS             complement(2077..3051)
                     /locus_tag="MB219_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCC8427460.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="MB219_001746"
     CDS             3334..4302
                     /locus_tag="MB219_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8427461.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="MB219_001747"
     CDS             4415..4693
                     /locus_tag="MB219_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCC8427462.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="MB219_001748"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="MB219_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCC8427463.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="MB219_001749"
     CDS             5150..5905
                     /locus_tag="MB219_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8427464.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="MB219_001750"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="MB219_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCC8427465.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="MB219_001751"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="MB219_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCC8427466.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="MB219_001752"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="MB219_001752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCC8427467.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
BASE COUNT         3260 a         1727 c         2099 g         2705 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa a
//
LOCUS       DAHEYX010000107         9776 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHEYX010000107 DAHEYX010000000
VERSION     DAHEYX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.108"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..784)
                     /locus_tag="MB219_001753"
     CDS             complement(<1..784)
                     /locus_tag="MB219_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCC8427468.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEI"
     gene            930..1733
                     /locus_tag="MB219_001754"
     CDS             930..1733
                     /locus_tag="MB219_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCC8427469.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1911..2858
                     /locus_tag="MB219_001755"
     CDS             1911..2858
                     /locus_tag="MB219_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCC8427470.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2926..3219)
                     /locus_tag="MB219_001756"
     CDS             complement(2926..3219)
                     /locus_tag="MB219_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCC8427471.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3247..3912)
                     /locus_tag="MB219_001757"
     CDS             complement(3247..3912)
                     /locus_tag="MB219_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCC8427472.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4052..4684)
                     /locus_tag="MB219_001758"
     CDS             complement(4052..4684)
                     /locus_tag="MB219_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8427473.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4691..5758)
                     /locus_tag="MB219_001759"
     CDS             complement(4691..5758)
                     /locus_tag="MB219_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCC8427474.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(5755..6486)
                     /locus_tag="MB219_001760"
     CDS             complement(5755..6486)
                     /locus_tag="MB219_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCC8427475.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(6654..7133)
                     /gene="greA"
                     /locus_tag="MB219_001761"
     CDS             complement(6654..7133)
                     /gene="greA"
                     /locus_tag="MB219_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCC8427476.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7269..8444)
                     /gene="mltG"
                     /locus_tag="MB219_001762"
     CDS             complement(7269..8444)
                     /gene="mltG"
                     /locus_tag="MB219_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCC8427477.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            8609..9769
                     /locus_tag="MB219_001763"
     CDS             8609..9769
                     /locus_tag="MB219_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCC8427478.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         2957 a         2016 c         1539 g         3264 t
ORIGIN      
        1 caatctcgct tagtagctga tcttcaaaat caatatgtcc gtcgaaaata attatctttt
       61 cgctatactg ccaaatatgg acatgatgaa cattctttac cccatcgatt gcttcaattt
      121 cttgttccat tttttcatag tcaatagcag gtgcactttg tatcaaaata gcggcagctt
      181 ttttgatcac cattatcgct tctatcaata tatatatcga aatgatcagt gtaataactg
      241 gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc tccgataatc acacttaccg
      301 aagacagtgt atcacttaac aaatgcaaat aactagattt tatattcaaa ctttcatctg
      361 cttcttgtcg taataataat actgagaaca aattcgcgag caatccaata actgctactg
      421 ttagcatcaa atgactattt atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg
      481 ttaaaaacaa ggataatatg atcaatatga atgagttgat gaaagcactc aatatttcta
      541 aacgctggta gccatacgta cgttttttat ttgcttcttt tcgggccatt ttttgtgccc
      601 cgtaagaaaa aataattgcc aatgtatcac taaggttatg gaaagcatct gaaacaagag
      661 acaaactccc tgacataatc cctccaataa attctgataa agtgatcacc gcattcaaaa
      721 ccactactcc aaatagtttt tttgatgatc tctgtggctg tgtaggcact attccttcct
      781 ccattttatt tatattggat agtctttaga caagaataac atttttttga tatttcacca
      841 atcattcaga tgtttcccta aagcacttgt atttcacttg atacttcgct agaattgcaa
      901 gtgatgacac tactagatga ggagtactta tgacaacaaa acaattaatt atcagttcct
      961 ttatacgatt caaagaccta aaggaggcga gaaatgtccc attttggaaa gccattgtct
     1021 atcttttagt tttgagtata attatggctt tgccaatctc ttttcaagtt tttcaagtct
     1081 taaataacat tcaattggat gggcaaaaga ttgctactcg cattcctgat tttacaatcc
     1141 aaaacgggca gcttcaaacc gaagaaaaag aaggattcat ttaccaaaca aactccatta
     1201 tcttcacatt tgatccagag ggaaaacgaa cagaacagga catctctact gatttgatgg
     1261 ggaattttgt tagtgtcggg atgttaaaaa acaaacttat catagctctt cctaatacag
     1321 gcacgacttc tgctttgttg aacaataacc aattagaact tccctacacc aatgaatctt
     1381 taaaaaactt aacaggtaag cagctccgtt cttttttaag tgaagcatct ataccacttt
     1441 ggataaaagt cctgacattt atattttcta tttatcctag ttttctgaat ctgctgatca
     1501 ctttgctatt taccaacatt gctgcttttc tctatgcacg ctttcgacta acaaaagcta
     1561 cttttcttga ttgtttaaaa acattgatct attcaatttc attgcctact ataattgcaa
     1621 caatcttgat ggtctttctt ccttcttttg acactagcgc cttcattgct tttgcaggaa
     1681 tctttatttt tgctcaagct gtcaaaggat gggcaaaaat atcgatcaat taagaaataa
     1741 agacaactcg ttatttctac ttaaatagac ccttttagag gttgtgacat ttttttgtca
     1801 cagccccttt tttatttgaa aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag
     1861 ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg gaaattaact atgtcagaaa
     1921 gaatcattta tgcagatcgt ccatttcgtg atgaatttat cgaagaaatc catactatag
     1981 cgcctggtta tatctttaaa acagaattac aaccagagga tctacaaaat gtagagatca
     2041 gtttgggctg gaactcagac taccaagaaa aactattagc ctcttctacc ttgaaatggg
     2101 tccaatctat ctcagctggt gttgatcagc taccccttga gactttttca aagaaacata
     2161 ttttactttc aaatggtagt ggcatccaca gtgaatcgat tacggagcat gtcttaggaa
     2221 tcatccttgg gtattctcgt ggtctttttc aagcacaaaa ggcacagagt gacatgaaat
     2281 ggttaggttc atcggtccat taccaatcta tccaagggaa aaaaatacta atcattggaa
     2341 ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc attagacatg aactgctatg
     2401 gaatcaatac atccggtcat ccagcagaag gaatggaaaa gactttttct ttaaatcaac
     2461 taaaggaagt tcttcctgaa gtagatttcg tagtcaatat cttacctcta acggaggaaa
     2521 caactgggct ctttgatcag catatgtttg aacactttga tccaaaatct gtattcatca
     2581 atgttggccg tggtgcttct gttaagacac aagatctggt gcaggcttta aataaccagc
     2641 aactttcctt tgcagcatta gacgtttttg aggaagagcc tcttccacaa gatcatcctc
     2701 tttggaaaat ggacaatgta ctgattactt cccacattgc aggtttgaca cctgattttc
     2761 aaaagaaatt aatggctatt tttttaacca atctgaaaag ctactttgcc acccatgagc
     2821 ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata gctgaaaaca atattttcaa
     2881 aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc tttttttatt gttttttaaa
     2941 gtgataggtt ggctcctcca ggttgggatc ataatcaaca agtaaatcat atcctttaaa
     3001 gaaccattca tctgcttcct cgataaaaaa aagtgtccca tccatttcca ctttctttac
     3061 aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt
     3121 gccgtagact tttccaaaaa aacgtattcc gtaagtttct ggcagatcaa tttctgagtc
     3181 aaaccatttt tgtgcttttg gcgttaccgt taatttcatc ccaacaacct actttctctt
     3241 ttctttctat tgattcatct tatcgtctag aatatccaca tcgcctgtct ctccaataac
     3301 cagtagatta tcattttcac gaacatattc atctgctgca ggagaagcga taaccgtttg
     3361 atctactctt cgtatggcga ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc
     3421 taatgttttg ttgaaaaatt taggattgga gactcggatt tctgctaaag aatatttatc
     3481 tgacagctct aagtagtcta gaatattctt tgatactaag ttatgtgcaa tccgtatccc
     3541 catgtctcgc tcagggtgta cgacacgatc tgctccgatc ttctctagta cacgggcatg
     3601 gtactcattt tgggcttttg ctaaaatatt cggtacgccc atttccttta ccatcagagt
     3661 aactaagatg ctggcttgga tatcttctcc aatagcgaca attacatgat cgaaatttct
     3721 tatacctaat gaacgaagtg tcatctcatc ctgtgcattt gccacaacag catgggtggc
     3781 tatattcata tattcattga ctcgatcttc attactgtca atagccagta cttcttgtcc
     3841 agcttcgatc agtgtacggc agatgcttcc gccaaatcgt ccaaggccta caattgcaaa
     3901 attttgtttc accattttcc tcctttagta tcttcttttc tcagtataac aaagcctttc
     3961 caaaaacaaa agcaagcatc attattgtaa aaaagccaag aaaaataata gttattttct
     4021 cagcttttga ttatcctctt tatcatcgtt tttatttcac caaaccatgt ttaaacgcat
     4081 agatagctgc ttgagttcgg tcttctacct ctaacttagc taatatattc gacacatgcg
     4141 ttttgactgt tttcagtgtg ataaacaatt catccgcgat ttcctgatta cttttccctt
     4201 cagaaatcag catcaaaatt tcattttctc gattcgttaa ttcttcatgc aatactggat
     4261 catttcgccg actcattttt tccatcattt ttgttgtcac ttcaggttct agtactcgct
     4321 cgccgcgttg cgtagccctt atagcatcag ctatttcatg tgctgtagac gtcttcaaca
     4381 aatatcctgc tgctcctgct tcaattgcag gataaacttt ttcatcatca ataaaacttg
     4441 tgacgataat gattttggct tttggccaat cttttaagat tgcttttgtt gattcgatgc
     4501 catccatttc ttccatgact agatccatta aaatgacatc cggtcgaagt gccatcgcct
     4561 tttcatagcc ttgacgtcca ttttcagctt ctccaatcac ttcaatgtct tcttggatag
     4621 ataaataaga agagacgcct aagcgtacca tttcatggtc atctactaat aaaactttta
     4681 tcatctttat ctatgcctcc ttcatcaaag ggaccttggt ctccacgctc gtaccttgcc
     4741 ctttaaaact aatgattttt actgttccac caataccagc tactcgttct ttgatattgt
     4801 tcaatccata acttcccgtt ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga
     4861 tacgtaataa aagattgtta tctattttat gcaaatagac ttctaattca ttagccttag
     4921 catgtcttaa tgtgtttgat aacaattctt gaacgattcg gaacaagtga tcctcaatgg
     4981 aactagttaa ttttacatct tctacctccc attttagcga aatctggatt ttattctgca
     5041 attccttcaa taactgctca atgccttgtt ttaaactttt ttcttctaaa ttgactggac
     5101 gcagatgaag aagcagcgca cgcatctcag actgggaagc gttgatgatc tctgccacca
     5161 ttttcaactg cttacggaac atttctggtg tctcactttt ttctgcttgc tccgtcaatg
     5221 ccgacatcat catcatcgct gcaaataatt gctgtgaaac tgaatcatga agctcacgag
     5281 ctaatcgatg cctttctaat tttaaaatct cttctttggt ttgtccatct acatattgag
     5341 gacggctagt cataatctgt aattcactgg atacttcgat cattttttct tttattttag
     5401 taatttccat atcaatatct ttgatgtaca gatcatccga cgcgcttggg atcggctgat
     5461 gcaaaagttt ggattcataa ttgccagaag aaagtgcacg aagcttttct tcgattttcc
     5521 cgtattttgc cttttgaata agtgataaca atagaaatac gatcaatcct acacctaatg
     5581 aaattgccat caaatggaag ataagcggaa cgtataataa acgcgcacgc agcaattctc
     5641 cccaccaatg actttggtta ctggcataaa aataagtgaa aagtgaaaat agaataataa
     5701 ggaaagccgc aatccccgaa taaacagcta gcattgcttt ggatattctt cccatcatat
     5761 tcgtatcacc tcgacatctc caaccaaagt attggtaatg atcttcaatc tgcgaggatt
     5821 ttcatcataa tcactgctgc agattttgat ctgttcgttt tttaaggaaa actgctcttc
     5881 ttcaaacaag acatttccta ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg
     5941 tactaagatc cgtgttctgc ctatcccttt tcttacaatc acaatattat catcttttgg
     6001 caaaagtgta ttgcccaaat cgatgatagt atcaccagaa atgatggcaa tgttgatatc
     6061 atcccattca tagacgtcgt ttccaatacg ttggttacca aataattgct gtcttttacg
     6121 ctcattatta tgtgttttta attgctctgt ttggaccatc atgatctgtt tttttcgcca
     6181 aaaagcattt tttgtcaaat ctattcctga aatctctaca cctttcagcc cgataaataa
     6241 aacggccaat acaaccatca tccataaagc aggactgttg actaagctaa aaaaaatgac
     6301 caagctccct aagatcaatt gaaaattttg aaatttagta cgaggatatt tgcgcatagc
     6361 tagatatata ttgaaaatac cgaatattac taaaagcaaa agtccagtat tgtttacgat
     6421 ttgccaaaca gcaaataaca gcagcagtgc ttcgactacc acaaaaaaac gccaagaact
     6481 attcatcgct ttttctccct cctgacacat tctcatttca tcatacctat ttttttgact
     6541 gttggctata ggactttaga tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa
     6601 aagatctaga gtgcgtcatc agtaagatga actctctaga tctttcagtc agactacgct
     6661 tgttctactt tgacgatttt aactaacata tcgccacctg gtgtagcaat ggttacttca
     6721 tcatttttac gtttgccaat caatgcttgc gcgataggag aatcattgga aatctttcca
     6781 gaaaacgggt ctgcttcagc acttccgaca atcgtgtact cttcttcatc tccatcaggc
     6841 aactcgatga acgtaactgt tttaccgatc gatacctcat ctgcatctac gccgtcatta
     6901 tcgatgattt gtgcaaaacg gatcatattt tcaattgttg tgattcttcc ttcaacaaat
     6961 gcttgttcat ctttagctga ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga
     7021 gcgattttga ttcgttcaat gatttctccg cgtttaactg tttttagttc ttctaattct
     7081 ttttctaatt tttctttacc ttctagagtc attggataaa ctttctctac catgtgtaac
     7141 gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa
     7201 agattaccac gcttttctta aaatgtaaat gtttttctaa aaatgatcaa tgactagccc
     7261 ctgtttttct aagaattatt tacatatttt tgcactagtt ccatatgttc ttcatacgtt
     7321 ttagcgaaat aaacatttcc agtgctcgta tctgctacga aataataata gtcattttct
     7381 gctggttcta atactgcctt gattgcctct tcgcttggat tgtcaaaagg accaggccca
     7441 tagccggtat tggtatacaa attgtatggt gaatctacag ctgtatcttc atacgtcacc
     7501 aattctttat gttctcctaa tgcatataaa acagagatat ctgactgaag tggcatattt
     7561 gccttgattc ggttaaagaa gacttgtgca atatttttac gatcattttc ttttacgcct
     7621 tctttttcaa ctagagaagc caaagttaaa acttcttgta cagtcaaatt tttctgtttg
     7681 atagctgaat aatagttttg catgaccgta tttgttttgt tcaccatctc aatcacaaga
     7741 tccttaagcg aagtactcgt gtagtaatca taagtagctg ggaataagta gccttccaaa
     7801 cggtattttg tgttctcagc tttagaggca ctttccaaca attctgggaa tttttgatac
     7861 aactcgttaa agaaagtctc atcatttact aaatctaaaa attctttctt gtctttacca
     7921 gttaccttcg ctacccgctc agcaatctga tcgatgtcgt agccttctgg aatggcaatt
     7981 ttcccatctg caacttttgt tggctcagaa gtgccgcctt cttgcagctg tttgccaatc
     8041 tcgtcaagag tcatattagg cgctaactga taatagccag cttgaaagcc agttaaattt
     8101 ttaaattttg tataataatt gaaaacaatc ccgcttttaa taatattgtc cttctccaaa
     8161 atctcaccaa tctgcttgtt agaagagcca ctcgggatct ccacttggac taattggtca
     8221 tctgatttat ctaaaggctt caacccgcta tctacataac gatacacggt aaatccaaga
     8281 aatccgccaa taattaataa tgtaagagca atcactaaaa tgatcttcct cacaatctta
     8341 tcttcttttt tacgcagatt tctccgcttg tcgattatat ctgctgtagc tgccttactg
     8401 gcttcttttt tatctttact tgggtgttgt ttgtttcctg acaaaacaac tcctccttac
     8461 tatcatcttc gtaactctgt ttattataca tgaaatttct actgccgtga attattttac
     8521 caaatattat gtcaaaattg taagtggcaa atactctttt tgaggattac aatgaaaaat
     8581 agttaaaaat ggatagaaag gaattaacat gtcaaaagga aaacatcgaa aaaaacaaaa
     8641 aatttctatt tcttttcttg taattttatt attgtctgtc agcttctttt caacaggcat
     8701 cattatagga ggtcaattaa caaaaatacc tcattcccaa acttataaag cggagccaac
     8761 acaacttact tccaatttaa cgcccgatga gcagaggctt gctagcaaaa ttgatcaatt
     8821 actacaaaaa tcagattata tcggttcgat ttatgttcgc aaaaataatc gcgtactatt
     8881 agaaaaagga tatggatatg caaatctgcg cgtaaaagaa tcgaattcac cttctattta
     8941 ctatcaaatc ggttcgattc aaaaagcaat gactgcttta ctgatactaa aacaaatcca
     9001 agctggtaag ttaacttttg acacaaagtt agcagaattt tatcctcaga ttcctggaag
     9061 tcagcaaatc acaatcaaaa atatgcttta tatgcgctct ggtcttcatc gaactgcttc
     9121 tcccgacaaa cctatgtcgg atagtgaaat cattcagttc gcccttcatc atttgaaact
     9181 tactgattat gatacgtatc attatgaacc attgaattat acattgctta cagggatatt
     9241 gatcaaacta acgaatcagc cttatgaaac tcttctaaaa aaagaaatca ttcgtccact
     9301 ccatctagaa catacagatt tttatgacaa tgtaaaaaat tcgcctcaac atgctgtatc
     9361 ttaccagatg tctgcaactg acgattattc aaaagcattg atcgagcctg aaaccgacat
     9421 ccgaaatgaa ttaggtacag gaaacataag tatgtctgtt tatgacttga acaaattctt
     9481 taccagtgtt ctttctggcg gtatcatacc aaaagaacta cttttctcat tgtggcaaaa
     9541 taatggtgat aaacatcctt acagcggcgg tgtttacagt ggaaacgatt atatccttgc
     9601 tcaggggaat atcaatcgat tccatgcagt agtggcgatg aaaaaagaca ctaaagatgc
     9661 aatcgtcatg gaaagtaata tccaatcgga taagaacatc aaattgccgg ccactgattt
     9721 aagaaatcag atttatgaac tgatggaagg tgttcactta tcaaattaaa ttttga
//
LOCUS       DAHEYX010000108         9636 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHEYX010000108 DAHEYX010000000
VERSION     DAHEYX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.109"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(22..342)
                     /locus_tag="MB219_001764"
     CDS             complement(22..342)
                     /locus_tag="MB219_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCC8427479.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(490..2724)
                     /locus_tag="MB219_001765"
     CDS             complement(490..2724)
                     /locus_tag="MB219_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8427480.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3203..3469
                     /locus_tag="MB219_001766"
     CDS             3203..3469
                     /locus_tag="MB219_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCC8427481.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3469..5196
                     /gene="ptsP"
                     /locus_tag="MB219_001767"
     CDS             3469..5196
                     /gene="ptsP"
                     /locus_tag="MB219_001767"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCC8427482.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5360..6406
                     /locus_tag="MB219_001768"
     CDS             5360..6406
                     /locus_tag="MB219_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCC8427483.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6423..7646
                     /locus_tag="MB219_001769"
     CDS             6423..7646
                     /locus_tag="MB219_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCC8427484.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7735..8622
                     /locus_tag="MB219_001770"
     CDS             7735..8622
                     /locus_tag="MB219_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCC8427485.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8708..8938
                     /locus_tag="MB219_001771"
     CDS             8708..8938
                     /locus_tag="MB219_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCC8427486.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8995..>9636)
                     /locus_tag="MB219_001772"
     CDS             complement(8995..>9636)
                     /locus_tag="MB219_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HCC8427487.1"
                     /translation="VLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLAL
                     RAQLPDLQEMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIE
                     LVTYFIRRIQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDF
                     YYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         3069 a         1708 c         1967 g         2892 t
ORIGIN      
        1 attattatgt tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat
       61 cgagatttca acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc
      121 gtctttgatt ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt
      181 acgtttgtca ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt
      241 ggtatttccg gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa
      301 tacaagacgg gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat
      361 gtatttttaa ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt
      421 tgacttttct gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata
      481 aaactggatt tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga
      541 agtaacgatg atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg
      601 atcaagataa aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg
      661 acgagcaccc atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg
      721 aacttcgatg tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt
      781 gacgattgtc atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg
      841 gttcagaaat tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc
      901 ttcgcgtgcc gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt
      961 cataatgatg atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc
     1021 atcatctaag atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc
     1081 taacaagaca agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc
     1141 gtatccgaca taacctggag gagaaccaat caatttagag acactatgtt tttccatata
     1201 ttcagacata tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg
     1261 tttcgcaagc tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg
     1321 acgattctgt ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac
     1381 agcgttatct tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt
     1441 ctgttctttt tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc
     1501 catatctttt tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt
     1561 ttgaagttta ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga
     1621 agctaaaact ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac
     1681 gatttggatc gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc
     1741 cggcaagaag cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc
     1801 atcagtatat tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact
     1861 gatcgtttct tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc
     1921 agcgtctttt tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc
     1981 tcctcgagct aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga
     2041 accagcaccg acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg
     2101 gatctcttca atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg
     2161 gactaaagat acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc
     2221 accatctacg attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc
     2281 tccaatcaag acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat
     2341 ttcgtcatcg cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt
     2401 gattccgtat tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa
     2461 gttgttgttt ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat
     2521 ttggcgagac atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg
     2581 ggccatatta gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca
     2641 gtaatcgagt tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt
     2701 cttgccgcag ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc
     2761 catattttac atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt
     2821 tatgaggtag aaaaggtgaa atctctattg accattcctg acctttgact cttattatac
     2881 taaaaataaa aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa
     2941 agaatgataa acgtcaggag aagagaaagc gacaagaaaa agaaaataaa cacgtttgtt
     3001 ataaaatgtg aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa
     3061 attgaatgaa aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt
     3121 ggcatttgtt aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact
     3181 cacttaaagg agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg
     3241 gatccacgct cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt
     3301 aaacttagag tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt
     3361 aggtgtaggt caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc
     3421 aatggcagca atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg
     3481 ctaaaaggga ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa
     3541 ccggatttaa cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg
     3601 gatgctgcat tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa
     3661 agcttaggtg aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca
     3721 gaaatggtag gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg
     3781 ctaaaagaag taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat
     3841 atgcaagagc gtgctgcaga tattcgtgat gttgctaaac gtattttagc gcacttatta
     3901 ggcgttactt taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac
     3961 ttgactccaa gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac
     4021 attggtggac gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc
     4081 gttggtacaa aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg
     4141 atcgaaggcg atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca
     4201 ggtgcagatt acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc
     4261 acagctgacg gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt
     4321 ggtgtccata ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg
     4381 gattctccag acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa
     4441 ggtatggaag gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta
     4501 ccatatctgc aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc
     4561 agtttatctg aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca
     4621 tctgttcatg gtaacttacg tatcatgttc cctatggtag ctacactcaa agaattccgc
     4681 gcagcaaaag cgatctttga agaagaaaaa caaaaattga tcagtgaagg caaggaagta
     4741 tctgatacga tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac
     4801 aagtttgcaa aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca
     4861 atggcagcag accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca
     4921 attttacgtt tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt
     4981 atgtgtggag aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta
     5041 gatgagttct caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg
     5101 gacacagcga aaatggctga acttgctgat cgtgcattga aagaatgcga tacgatggaa
     5161 gaagtcgttg aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg
     5221 cttgtaaaag agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa
     5281 gaatagataa aaatagggct taagtttgag tgacagtttg taggctatgt tatactatat
     5341 aagtgaaggg ggacgtaagg tgaaggtatt attatatttt gaaagtgaaa agatgcttgc
     5401 aaaatctgga atcgggcgtg cgcttgatca tcaaaaacgt gctctgacgg aagtcggtat
     5461 ctcttataca ctagatgaaa aagaagatta tgacatcctg cacatcaata catacggaat
     5521 caatagtcat aatatggtca ataaagccag aagagaagga aaaaaagttg tctatcatgc
     5581 gcattctaca gaggaagatt tccgaaattc ctttatcgga tcgaaccagc tttctcctat
     5641 cgtaaaaaaa tatctggtag gattgtacca aaaagcggac tatctgatca cgccaacacc
     5701 ttattcaaaa cagttgttgg aaagctatag tatccgtgtg ccgattcaag ctatttccaa
     5761 tgggatcgac ttagagaaat atcgccctga tccaatgaaa gaacaaaagt tcagagaata
     5821 tttcaaactg tctccggatc aaaaagtaat catttgtgtc ggtttgtttt tcgaacgtaa
     5881 aggaatcatt gatttcgtag aaattgctaa gaagatgccc gaatacacat ttatttggtt
     5941 tggtcatgtt cccatgtact cgattcctcg gaacatccgg aaaattgtaa aagaagatca
     6001 tcctgaaaat gtcctgtttc ctggttacat tcgtggagag atcatagaag gtgcgtattc
     6061 aggtgcagat ctattctttt tcccttctta tgaagagaca gaagggatcg ttgttttaga
     6121 agctttagca agcaaacaaa acgtacttgt tcgagatatt ccagtgtata atggctggtt
     6181 agaagatagc aagaactgct atatggggaa gacaaacgaa gaatttatcc gactgatcca
     6241 acagattacc aatcaagaac taccgagtac gacacaagcc ggttatcaaa cagcaaaaga
     6301 gcgaagtatc aataaaatcg gtgaagaact taaaaatgta tacgaaagtg tactgaatga
     6361 aaaagtctct agcaaattaa aaaaagtaaa tcaggtcaag gactagtttg gaggcgaaaa
     6421 acatgaaaat cggctttttt accgatacct actttccgca agtcagcgga gttgctacat
     6481 cgatcaaaac attaaaacaa gaattagaaa gacatgggca tgaagtctat atttttacaa
     6541 cgaccgatcc taatgcggat aagttggaaa aagatgtgat tcgaatgcca agtgtaccat
     6601 ttatttcttt caaggatcga agagttgttg ttagaggcat gtggtatgct tatttggttg
     6661 ccaaagaact agaactagat cttattcata cccatacaga attcggagca gggatacttg
     6721 ggaagatggt agctaagaag ttgaaaatac ctctgatcca tacttaccat acgatgtatg
     6781 aagactacct tcattatatc ggtaaaggaa aagtgatcag gcaatcccat gtaaaatact
     6841 tgtctagatt atttgcaaat catacgacag gtgtagtttg tccaagtgaa cgtgttattg
     6901 atacattacg tagctatggt gtaatagcac cgcttcgcgt gattcctact ggtatcgaca
     6961 ttgaaaaatt caaacgttct gatatcactc agcaggatat cagcaaatta agggagtcac
     7021 taggtcttca ggaaaaccat ttgatgattc tttctttaag caggatttct tatgaaaaaa
     7081 atatccaagc cttgattaat gggtttcctc aggtcatctc cgcttatcct aacgccagat
     7141 tagttatcgt aggaaaagga ccttatgtag aagagttaga ggaattgatc tcagaactgc
     7201 agttagaaga atttgtgcag ttcacaggag aagtggacaa caatgatgta gcactttatt
     7261 ataaagcggc agattatttt gtcagtgctt ctacttcaga aacacaaggg cttacttata
     7321 cagaagcgat ggcagccgga acaccttgtg tcgtggaagg aaatgcttat ttgaaccgat
     7381 tgttcgatca tccttcatta gggaaaactt ttgaaaccga cgaagacttt gcaccagcac
     7441 ttattgatta tatttccagt gaagttccta aagatccaaa agttttagaa gctaaacttt
     7501 atgatatttc atctacacat ttcggcgaag ctatgatcga attttatcaa gacatggttg
     7561 tttatcatga agaagagatg cgaagaaaag aagaagaaga ctcgattgaa cgaatcaaaa
     7621 taaaattaaa ttcatttaaa agataaaatt caaacatttt ttatgaaaaa atccttgttt
     7681 ctagcgtata ctagcataga agcaaggatt tttgattttt aggaggtcga aaaaatgaaa
     7741 cttggtttta tcggaacagg cgtcatggga agtgctgtcg cacgccatct actagaagca
     7801 ggacacgaag tagctgtcta taatcggaca aaagcaaaag ctgatccttt agtaacagaa
     7861 ggagctatct gggcagatac accaaaagcg gtagcagaac aaagtaatat tctctttaca
     7921 atggtaggtt accccaaaga tgtagaagaa atctactatg gacagtcggg gattttttct
     7981 gcagatatct caggacatat tttagttgac ttgactacaa gtacaccaag cctggcagaa
     8041 aaaattgcaa aaacagcaaa agaaaaaggc gcagatgcgt tagatgcacc ggtatccggt
     8101 ggtgacttag gagcaaagaa tggtacactg acgatcatgg tcggtggaga agaagccgtt
     8161 tatgatcaag tactgccttt attcaaagaa tttggtacga catttacatt gcatggttct
     8221 gctgggaaag gtcagcatac aaaaatggct aatcagatca tgattgcagg tacgatgaca
     8281 ggaatgacgg aaatgcttgt ctatgcacaa aaaaacggac ttgatttaaa gaaagtgatt
     8341 gagacgcttt caggtggaag tgcagctaac tggtctttaa gcaattattc tcctcgtatt
     8401 ttaaaagaag attacacacc tggttttttt gtcaaacatt ttatcaaaga tttaaagatt
     8461 gcattagaag aagcagaaaa aatggacctt gtattgccag caactaccca agcattgaaa
     8521 ctatatgaag agttggcaga taaaggtttt gagaatgacg gaacccaagc tttgatcaaa
     8581 ctttggtggc ctgaaggaaa aataccagaa aaaaaatcat aaaaaacgag tttttttaga
     8641 gaaaatcaat gcaagtaggc tatgcttttg atacaatata acagaaataa gaaaaggagg
     8701 acctccgatg acacattcac aattagtcgc aatcataaaa cgactggagg ctatggtcga
     8761 gtcagcagat aatgaactgc aagttcgccg ttttgaaaaa gaaggcgtag agaagtgtac
     8821 agtaacttat gataaagcga ccgatacatt tgaattaact gaaacagata ctcatcagca
     8881 atacgaattt gataatatcg atatcgtagc gatggaaatc tatgacttaa tccaataaat
     8941 tttagaataa ttgacatttg aagttgaaga tgcctaaaag aagccgagaa gcaactaatt
     9001 tagttgcttt tcagcttctt ttttgtttaa cagttcaatt tcagactgtt ctttttctaa
     9061 ttttttgcgg taactgatca agatttgtac aggatcgatt tcttcttttg acaattgacg
     9121 tttcaaagat aaatgagctt ctttcttttc ataatagaag tcaggagaaa cgtcagcaga
     9181 ggcaatcatt gtttctgcaa gttgaccata acaaacgagg tagtgctttt tctgactctc
     9241 atctgctgct aatggcatgc tgaaggtgag cgatggtgtt cttaaaaaat gcttttgttc
     9301 ctggatccgt ctaataaaat aagtaactaa ttcgatatct ttttgaagca taccggcttt
     9361 ttcgaaattc aactgataga tgaaatgttg ttgtcttttt ttcaagtaag ttagtagctt
     9421 tttattttgg ccttgaaaca gcccttggag ttcctttttt ttctgttcaa aagtcatttc
     9481 ctgtaggtca ggaagctggg ctcgcaaagc taacaaacta atttcagaca accaaggcat
     9541 ttgataagtt tcttccatta tacgcaaaat agacggcatt tttttataga gacgaaaagg
     9601 cccgtatgtt cgagccgtag gagtatctgt taatac
//
LOCUS       DAHEYX010000109         9423 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHEYX010000109 DAHEYX010000000
VERSION     DAHEYX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.110"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            152..793
                     /locus_tag="MB219_001773"
     CDS             152..793
                     /locus_tag="MB219_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCC8427488.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            836..1528
                     /locus_tag="MB219_001774"
     CDS             836..1528
                     /locus_tag="MB219_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCC8427489.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMEQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQDFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            1525..2427
                     /locus_tag="MB219_001775"
     CDS             1525..2427
                     /locus_tag="MB219_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCC8427490.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQLG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     LDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2585..4291)
                     /gene="efbA"
                     /locus_tag="MB219_001776"
     CDS             complement(2585..4291)
                     /gene="efbA"
                     /locus_tag="MB219_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCC8427491.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIAELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFHRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKNFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTEYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4481..5104
                     /locus_tag="MB219_001777"
     CDS             4481..5104
                     /locus_tag="MB219_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427492.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIRQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5554..6603
                     /locus_tag="MB219_001778"
     CDS             5554..6603
                     /locus_tag="MB219_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8427493.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6600..7481
                     /locus_tag="MB219_001779"
     CDS             6600..7481
                     /locus_tag="MB219_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427494.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7484..8254
                     /locus_tag="MB219_001780"
     CDS             7484..8254
                     /locus_tag="MB219_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8427495.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCKEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8533..9387
                     /gene="aroE"
                     /locus_tag="MB219_001781"
     CDS             8533..9387
                     /gene="aroE"
                     /locus_tag="MB219_001781"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCC8427496.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
BASE COUNT         3031 a         1618 c         2021 g         2753 t
ORIGIN      
        1 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
       61 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
      121 gaaaaagaaa tctcaaaggg aggtaacttt tatgggattg aatgaattta ttttaggatt
      181 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
      241 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
      301 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
      361 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
      421 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
      481 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
      541 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
      601 caaaaataac ccggaaaaag tttataagga gatgtttatt gaggcagtga ctacgattaa
      661 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
      721 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
      781 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
      841 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
      901 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
      961 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
     1021 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
     1081 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
     1141 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
     1201 agtcgtgggt gtcatgtacc agctaatgga acaggaaggt tggctgtata atcaagaaaa
     1261 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
     1321 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
     1381 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagattttc ttctcccctt
     1441 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
     1501 ttattatttc gaggagtttg agcaatgaat atccagcaga tgaaatacgt ggtcgctgta
     1561 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
     1621 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
     1681 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
     1741 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
     1801 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
     1861 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
     1921 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
     1981 atcagaagat atttacaact aggagagcta agtggccgca gcattggaaa attcaaaacg
     2041 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
     2101 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
     2161 gaagacctta tcgagatcct tgatcaagaa acagtgattt atacgagtga tcgaggtact
     2221 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
     2281 gggattcgcg agcatttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
     2341 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
     2401 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
     2461 cgttaatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
     2521 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
     2581 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
     2641 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
     2701 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
     2761 ctaactcggc agcttctgta attgtttcat cggagggctt gtcactttta atgatgacgt
     2821 gcgaaccggg tatatttttt gcatgaagcc aatattcggt ttttttagct gtcttcattg
     2881 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
     2941 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
     3001 ggtatccttc tgaagtcaat tcttctttga ttgcttcgat atccattggg ccggcaattt
     3061 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
     3121 cgccaatcag cttaacagcg ttcttcagct tttggtaacg atggaaatat ttctgtgcat
     3181 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
     3241 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
     3301 gttcgccatc tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
     3361 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
     3421 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
     3481 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
     3541 gtgtgaaaaa atttttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
     3601 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
     3661 tcagctcttg agcggtatcg aaaccgattc cctgaaactg acgttggatt gcttttggct
     3721 ctagttcagt ttgggaaagc cgatgaaaga ttttttcttt ctcggagcta aatgggttca
     3781 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
     3841 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
     3901 agacgatcgt actatgacgt cccattaatt cagcgatcaa tatgatattt tgtagatcac
     3961 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
     4021 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
     4081 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
     4141 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
     4201 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
     4261 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
     4321 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agtaaaagtc
     4381 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
     4441 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
     4501 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
     4561 aagtaagtat tttcgacgtc ttgtgtttct gctcatctta tcattgctgt taacagttgc
     4621 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
     4681 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcagaca
     4741 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
     4801 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
     4861 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
     4921 atttattttt tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
     4981 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
     5041 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
     5101 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
     5161 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
     5221 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
     5281 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
     5341 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
     5401 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
     5461 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc taattataat
     5521 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
     5581 gtagtaatta ttttagttta tttagcaggc gcatttattt atggaaaagc ctcaggcgaa
     5641 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
     5701 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
     5761 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
     5821 aagctagcca caatgagtca acagctagta caatctgatc cagatgtctt gattggaatc
     5881 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
     5941 gctgtaacgg atcctgtggg ggctggatta gttgcttctt tagagaaacc tggtggtaat
     6001 gtaacaggtg tttctgatca gccgcctgtt gcttcacaaa tcaagcttgc ttctgatttg
     6061 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
     6121 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
     6181 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
     6241 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
     6301 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
     6361 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcagcagaa
     6421 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
     6481 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
     6541 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
     6601 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
     6661 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
     6721 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
     6781 gagtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
     6841 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
     6901 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
     6961 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
     7021 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
     7081 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
     7141 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
     7201 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
     7261 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
     7321 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
     7381 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
     7441 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
     7501 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
     7561 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
     7621 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
     7681 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
     7741 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
     7801 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
     7861 aaaagaagat tttttcagac tatgtaaaga agtcggtaat ggcctagagc aacacttgga
     7921 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
     7981 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
     8041 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
     8101 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
     8161 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
     8221 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
     8281 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
     8341 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
     8401 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
     8461 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
     8521 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
     8581 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
     8641 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
     8701 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
     8761 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
     8821 aagggtcgtt tgattggcta taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa
     8881 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
     8941 gcagtcactg ttcaagctgc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
     9001 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
     9061 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
     9121 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
     9181 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
     9241 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
     9301 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
     9361 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
     9421 cat
//
LOCUS       DAHEYX010000110         9384 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHEYX010000110 DAHEYX010000000
VERSION     DAHEYX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.111"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            35..1963
                     /locus_tag="MB219_001782"
     CDS             35..1963
                     /locus_tag="MB219_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCC8427497.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFSESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2101..2556
                     /locus_tag="MB219_001783"
     CDS             2101..2556
                     /locus_tag="MB219_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCC8427498.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3026..3295
                     /locus_tag="MB219_001784"
     CDS             3026..3295
                     /locus_tag="MB219_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427499.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3695..3949
                     /locus_tag="MB219_001785"
     CDS             3695..3949
                     /locus_tag="MB219_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCC8427500.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4038..5084)
                     /gene="holA"
                     /locus_tag="MB219_001786"
     CDS             complement(4038..5084)
                     /gene="holA"
                     /locus_tag="MB219_001786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCC8427501.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5122..7395)
                     /locus_tag="MB219_001787"
     CDS             complement(5122..7395)
                     /locus_tag="MB219_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCC8427502.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7388..7927)
                     /locus_tag="MB219_001788"
     CDS             complement(7388..7927)
                     /locus_tag="MB219_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCC8427503.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8063..8281)
                     /locus_tag="MB219_001789"
     CDS             complement(8063..8281)
                     /locus_tag="MB219_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCC8427504.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8278..8523)
                     /locus_tag="MB219_001790"
     CDS             complement(8278..8523)
                     /locus_tag="MB219_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCC8427505.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8639..9367)
                     /locus_tag="MB219_001791"
     CDS             complement(8639..9367)
                     /locus_tag="MB219_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCC8427506.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2902 a         1832 c         1645 g         3005 t
ORIGIN      
        1 gctatccttg ttttgacaag gaggtctaat aacgatgact aatcagaaaa aagcttttgc
       61 agcaacaatc ggctgccttt tatttatgat cttcggtttt atcttcgacc gattctctct
      121 taccatcacc ccactttttt atatcagtgc cattatcatc ggtggatata aacaaacatc
      181 ggaaggcttg aaagaattgt ggaatgatcg gacactgaat gtggatttat tgatggcttt
      241 agctgctatt ggtgcctgtt tgatcggtaa ttactttgaa ggagcaatgt tgacattcat
      301 cttttgttta agcggtgcat tagaggaata cacaactaat aaaagtcaaa aagaaattac
      361 tgctttgatg aatcttcaac cacaaaaagc ccaattatta aaagaaaatg gacaaatgca
      421 agaagtagat gtttctcaac taaaggtgga taacttggta tttgtcgcta aaggagcagc
      481 tgttccgata gatggctttt cagaaagcac acaggcaacg atcgatgagt ctgcattaag
      541 cggtgaatct gtgcctgtag aaaaatattt aggcgatgag atgtatgcag gaacgctgaa
      601 tcaaggaaat cccatcacga ttcgagtaac aaaaacgagt tccgaaactg tctttgctaa
      661 gattatccaa ttagtagaag aagcgcagaa tacgccaact caaacagctt catttatcga
      721 acgaatcgaa aataattatg taaaattgat tttgcttgct gttcctctaa tgatccttct
      781 cccccacttt ttcctaggtt ggagttggga tgaaagcttt tatcgaggaa tggtattatt
      841 agttgtggct tctccttgtg cgcttgtcgc atctgctaca ccagcttctc tagctgctct
      901 ttcaaattct gcaaaaaatg ggattttaat caaaggcggt atccatttag aaaagctttc
      961 tgaattaaaa gcgattgcct ttgataaaac aggaacgctc actaaaggaa aaccagttgt
     1021 aactgacagc tttttctttg atgaaaagga tttggcacaa caacttcttg ttgctatgga
     1081 acaaaaaacg acgcatccac ttgctcatgc gattttgcaa tatttcgatt gcacaatacc
     1141 tgaagaaatc caatacttac caatagaaga aattactggt tttggattac aaactaccta
     1201 tttaggtgcg caatggaaag ttggcaagca cgcatatgat cctgagacga tgataatcag
     1261 caaagagatt gcggaaatga tcgaacgttt ggaaaatcaa ggcaaaaccg tgatttatct
     1321 ttctaaagat cagcagttaa ttgctgtttt aggtctgcta gatattccaa aagcaaatac
     1381 ccaacaagta atttcctact ttaaatccca aaacatccat acaagtatga taaccggcga
     1441 ccattctggc acagcaaagg ccattgctga acaagtaggg attgaacatt actatgcaag
     1501 ctgtactccg gaagaaaaaa cacaactagt caaaaaagag aatgaacttt accaagtaaa
     1561 tgcgatggtt ggtgatggag tcaatgatgc gcctgcttta gcagccgctt ctttaggtgt
     1621 cgctatgggg caagggacag atgccgctat ggatatcgca gacatcgtct taatgaaaag
     1681 cgacttaaat aaatttgtct acagtcatcg gctatcttta aaaatgcggc ggatcatcaa
     1741 acaaaatatt attttttcga ttcttgttat tctcttgttg attgcttcta acttcctgca
     1801 atttttaaat ctacctcttg gagttgtagg acacgaagga agcacaatat tggttattct
     1861 gaatggcttg cgtttactaa gacctttacc tgatctttct gattcttcaa aaaaatgtaa
     1921 gtcttgtcca ctatacaaag cgcggctgtc ttctgtcaaa taagcaaagt gtttttttat
     1981 gattttgttt attttatgaa aaaaacagct tatgaatagg tcttgaagga tgaattccta
     2041 cttttgtaac tttctaaaaa catgttatga ttgtttcgaa gaaagtgagg aatatatttc
     2101 atggaaagtt ggcttttttt aggaattatt ttacttgtag caattttagc taaaaatcaa
     2161 agtctgatga tagcggcagc ttttgttttg attatgaagc tcataccagc ctcccgttcc
     2221 tttttccctt ggcttcaagg caaaggaatc aatattgggg tgacgatcat cacagcggct
     2281 attctaatcc ctatcgcaac aggagaaatc ggtctacgtg atctattaga agcctttaaa
     2341 tcccctattg gctgggtagc tgttttttgc ggtgggctag tggctgtctt atcttccaag
     2401 ggtgtaggat tgattgcttc tgatccacaa atcactgttg cacttgtttt cggcacgatt
     2461 ttaggtgttg tcttcttaag aggaatcgcc gccggaccgg tcattgcttc tggtatcact
     2521 tactgtatct tacaagtatt tgctttagga aaatagaaca aattttgtct attttaggag
     2581 attcttaaca cccccaattt actgatccgt gatatactga caacgaggtg atacaatgtt
     2641 cgaaaaatta aagtatttca taggaacaat cggctggttt cttctagcgg tacttttgat
     2701 tctgggctta gcttatctat tatttcctga tttgatacaa aatgtgtttg atactgcttt
     2761 ttgattgacc tattaaaacg atatagggga tgctcctgcg atgcaggggc tttttttaat
     2821 caatgttttg taataattta ggccgtgcca atgagacttc ctatagaatt cgatgctctt
     2881 ttttcttttt ggctcttctg ctcttttttg ttatactaga ttacagtgga caaaagaaag
     2941 aatgatcgca tccacttcaa cgaatatcaa aatggaagaa ttagtgcctg ttttcaggtt
     3001 actttgaaaa gaaaggaaga cgcttgtgga aacttttgaa ggtcttttaa ttgatacgta
     3061 cgttttacaa gaacagccaa tgatgctgac tgggatcaac ttgcagcttg aggaccgtgt
     3121 gatcaatggc ctgcttaaag atcgctattt ggcaaaatat atcttacgtt tgcccctaaa
     3181 taaatatgtg ctggaagtaa gtggcaaatg gaaccgtcgg aaccagttgg tcataaaaaa
     3241 aatgttgata aaaaatatcg atgaatacat taaaataatc ggtacaccag aatagccttt
     3301 actgatcaaa ggatactggt tataaaataa ctgcagtttc ttaatagaaa aacaaagaac
     3361 aacagggaat cgtatccctt ttcaaaaaaa actttgcaaa cggttctctt tgttatataa
     3421 taacttagta ccaaacattc ccattcaaac tttgtttaaa tagataaata aaaatctagt
     3481 ttggtttgac gctattgcct cccacaatag cgtctttttg atttttttca gcttgtacta
     3541 tccgttttta ctgaaaaaaa atcctaataa aagactgtta aggaaaaaaa cttgtcatgt
     3601 tcatattgca aagcacgctg cctttaggta taataatgaa tgttgaatta tgcggtaaca
     3661 cgcgtaagaa cttaagttat cggaggtgaa actaatgcca aacattgaat ctgcaatcaa
     3721 acgtgttcgt acaagcgaaa acgctaatgt gaaaaattca tctcaaacaa gcgctatgcg
     3781 tactgcaatc aaaaaatttg aagatgctgt agcttcaggt gctgacaacg tagatgcgtt
     3841 atataaagaa gctgttaaag ctattgatat ggctgaatca aaaggtctaa tccacaaaaa
     3901 caaagctaac cgcgacaaat ctcgtttaag caaaaaaatc gctaaataaa agaatcggct
     3961 tcggccgatt tttttatttg tcaaaaaaag gacaaaaaca aacgaccttc ccgcatgctt
     4021 tgccctcgtc tttcttctta tcgatttctt aacaactgcc ctgtgcgcaa aacgaatagt
     4081 tgaaaactta attctttttc gatttgtccc gttttgattt gataatccat ctcaactaat
     4141 tgatcaaaca actgtaccag caaatgttca tcgaattttt ttacttcctg catggcaagt
     4201 tttacacgat atggatggat tttcaatgtt tcagcaatat ttgcctgctg ataaccaatc
     4261 tttacaagaa attttgtctg taaaaataaa cgtaattgag aaagcaaaat cgcattaatt
     4321 ttgattgttt cttccccttg cataactaaa tcttcgtata aacgcaaagc ttgttccgtc
     4381 ttccctttta aaatatattg ggtcatgtca aaaatattat gttctaacgt tttaggaact
     4441 aattcttcta cttctcttac cgtgatttgc tgttgatttt gtccataaag gaccaatttc
     4501 tgtaactctc tcatcatttt ggataaatct aagtcggtca acctcaagaa taaatcaatt
     4561 gcacttcgtt ccattttgat tgaggaattc tctaaggtat tcatcagata ttgacgtact
     4621 tctttttcat tcaactgttg gacattgatg ataattgctg tttttttcaa ttgtttcgtt
     4681 acttttttcc gttcatccaa cttttcatat ggcgcaaaga atactaatac agtcgattct
     4741 aatggttctt tcaaataagc cgtcaataaa tcagtatttt gttcgattcc attgtttact
     4801 ttttcacctg ttaaaaaata aggattctct acgaatacta accgttgatc tccgaaaaac
     4861 ggcaacgtct ctgcttcagc tactacctca tctaaacttg cattttccat gtcaaaactt
     4921 aaaaaattta agtcatcttc gccctctaat tggatttttt tgacaatttc tgtccgaact
     4981 tgatcttgga gaaacttttc tgttcctaaa atcaagtaac aagatgcaag tttttcttta
     5041 cgtatttttt gtaactctgc ttgtatactc aaattactca ccactttcaa aaattatcct
     5101 accatgagca gatgagaatt actagtctat cattgtttcc actagcccat gaggatgaaa
     5161 aactggatgc cagcgatagg tgatcattcc ttgttgatcc gttcgccatg tttttatctg
     5221 attttcttct agtgtctcca atacttcgct gtgcgggtga ccataacgat tatttacgcc
     5281 gcaggatata atcccatgtt ttgcttcaat atgggaaaga aaatctgtgt tacttgacgt
     5341 acggctaccg tgatgaccta atttgactac atcggctttc agattaggat aaatcaaaag
     5401 caattgtttt tccattgctt gattcaaatc tcctaaaaat aaaaagcgcc tattgttata
     5461 ttccatccat agaccaatcg aatcttcatt tcttccctct cccttttcaa aaggagtcaa
     5521 tacataaaga tcataataac cgctgatcct gtccccatgt ttgatttcaa tgatctcagt
     5581 accatttttt tgagcttcag ataaatgcct ttgtaatggc tcatttcgtc cagcgcccca
     5641 accgatataa atttttttga tagtaaaatg ctgagataac gtgggtaatt ctcccacatg
     5701 atccgtatcg tcgtgtgtca agatgagttt atcgattaca cttatcccct gaccttttag
     5761 atatggaacg atatttcttt cagcaggagg acgtatcata ccttgcgccc acttttctct
     5821 ttcaaagtgt aactgacctc cagtatcgat caaaatcgtt tcttttcgaa aaggactttg
     5881 caaaaagata ctgtctcctt gaccgacatc gataaaagcg atcgttgaag aaaagcgtaa
     5941 aaatggcaaa gtcaaaaaaa taaaagaaaa aagaccaaca taaaacaagt gtttcacagg
     6001 ttgttctttc aacctatcca tacaaatcag taatccgaaa atatacatcc cagatagcac
     6061 aaggggcaaa cgtcctataa tgagagacga ccctgaagaa aaatcgatta ttcgctcaaa
     6121 taagattaat ccctcttcta tcagcaaaag taaagtttcc ggcagccagc gattgaagag
     6181 tgtgagaaat aacacaccag gaagaaaaag aaacttgaat accggtgcaa tcagtaatgt
     6241 aaaaagacta cctaaaatag gccactcata aaaatgccat acaatcaacg gcatcgtgat
     6301 catagtaaac aggacgtgag aggccgtttt tttcaaccaa tctccatcaa aagaaatcag
     6361 gataaacaaa aaactcatga aaaaactaag ctgtccaccc gcatgaaaaa gaaatcgagg
     6421 atcaaatagt aaaagaagcc aaaagacaac tgcaaataag tccatggtgg aataatgctt
     6481 attcagcaat ttgactagca atcgcaataa aaacagcaaa ctagcacgca aaacagaaat
     6541 agaacttcct gcaatagaaa cataacagat agttagtaga ctcaacggaa taatactgac
     6601 gtgtagtggc catccgatac gtcgaaataa ataatacagc caccctaaat aaaattggat
     6661 gtgtagaccc gatagactga acagatgcag caaaccactc tccctaaaaa tttcttctgg
     6721 ttctctgaat cctgcatctt tatagcccca taataaagaa ttaatatata cacttgtttt
     6781 ttgcggaaat tcagattgga caaaggagat aaaagatcct ctttttctct gaagccagtt
     6841 tcgccagtga ttttgcatgg tgatctgttc aatcttttga atttggaacc gtccagaaat
     6901 tctatggttc ctgaagtaag cttcttggtc aaaattataa agattccgct tttttgctgg
     6961 agagatgaac tcgccttcag cagtcaattc tattcccttt cctcggaagt taagccatgt
     7021 tttcaattct gcttctgtgt ttacttgata agaaagctgg agcttttgtt tattctctga
     7081 taaccttcct tccattgtca aaaaattacc atttgcacga atcgtatctg taaatacagt
     7141 aatttgcgca gatgttgttt tttcctctaa aaatgaatac tcttgtagcc tataagaaat
     7201 ggataccgtt atgctacaca aaatacacaa gtacgcgata aaacgtcctt cttttagcga
     7261 aaaccataaa aaaattccgg caagaagcaa acgataaccc caattgtcag tgtaatacaa
     7321 caaacagata atcatcgacg taattgccgg aaaaatcaac cgatttcgaa catgcctttc
     7381 taaaacgcta gacatgtttt cctgttaatt cattttgaag ttttgaaaaa tgctgtgagt
     7441 ctaaagcaac ttggtgtaca gatacaccta attgttcgat caacgcttgg gcataaggat
     7501 catttcgata gtcatggaga taattgatcc ctttcacacc agcttgcaac aaagcctttg
     7561 tacaggccaa acatggaaaa tgtgtgacat agatttctga accttccgtc gggataccaa
     7621 gttttgcaca ctgtaggagt gcattcatct ctgcatggat cgttcgcaag cagtgtccat
     7681 ccacgacgta acacccttca tctatgcagt ggacatctcc actgactgct ccattatagc
     7741 cgccagcaat gatccttttt tctcgaacga gtgtcgctcc tacttctaaa cgtgtacacg
     7801 tactacgtaa agataacagt actgcctgag ccataaagta ctggtcccaa ggaatgcgtt
     7861 cattatgtga cttatcagta actttttctt tattcgtatc gcatgctttt gaagattttt
     7921 ctaacatgtt ttatattcct ccgttttttc aagttgatta aaaagtactc attgcttaaa
     7981 caaaatattc tttaaatata acgaatcatc ctgtctgaca aaagatccta cccttcactg
     8041 ccgaaatgaa gtccgtcatc cattagccca ttataaaaat tcttcgaatc catttgttca
     8101 agaaattccg tataaaaatc gatacttcac aatcatggcg ttttgctact tctgtgagaa
     8161 cagagatata ttctttgatc aacttgttat tccgatattt ttgttttgtc tcatcgactg
     8221 ctggtggcgt gataaagaaa gcatgatcag aactatagtc ttttttcagc tgaactatca
     8281 tccactgcag attttttggt acgtttcttt agaaagttgt ttatgctctg ctagatcatt
     8341 cgtcccaatg agaataaata ctttatctgc tgtatagcta agtacatctt tggcaaagcg
     8401 ttctcgtaga gcttgactgt tattgccgct gacagccgta ttcataatat gaatcccaga
     8461 tattttattt tggagataga cattcagcat tggttttttt aatgtttctt tgcgaacaat
     8521 caagctattt cctatccaaa aatactacct cctcgtcctg ccaagctctt agaattggat
     8581 tattcttagt atccattagc gtccatttat tgctcgtgcc agtcgatttt gaatcattct
     8641 atttaattgt aacgaagttt tttagttttt caactgtctt ttcaccgata ccagaaatct
     8701 cctgcaggtc ttccactttc tggaaggatc cgttttcttc tctaaatcga atgatttctt
     8761 gcgctttgac tgatccgatc ccaggaatct gctgaagttc tgatggagtg gctgtattga
     8821 tatttatttt gggaggctcg gaatctgctg taggctgttt tccttccgag gatgccaact
     8881 gatcaagatg ttctggcagc tcttcaccta cagcagggat atataggact tgctgatctt
     8941 ctatcttcgc agaaaagttg attcgatcgc tatccgcttc ttctaataat ccacctgcac
     9001 gctttagtac atcatagacc cgttcttctg aagaaaagga atagatacca ggctccttca
     9061 cacttccttt gatatcggtg tacatgacct ttggacgagc atcctcttct acatcgtttc
     9121 cttcagctga tggatcttct ccactggaac taaacgaaga aagtatttct tttgcagcag
     9181 tctcttctgt ttctctataa aatagcaaat ttgataataa aaaaccactt gcgatacaaa
     9241 agataagaac tatcatagac atccaaatga tcaatttggg tgaagctagt attttcttgc
     9301 tgattttttc tatcattgtt cttttttcaa agaatgacca gtaaaaatca ttcattactc
     9361 ctttcattta aaattgaaga aaca
//
LOCUS       DAHEYX010000111         9331 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHEYX010000111 DAHEYX010000000
VERSION     DAHEYX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.112"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(84..1133)
                     /gene="recA"
                     /locus_tag="MB219_001792"
     CDS             complement(84..1133)
                     /gene="recA"
                     /locus_tag="MB219_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCC8427507.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1242..2483)
                     /locus_tag="MB219_001793"
     CDS             complement(1242..2483)
                     /locus_tag="MB219_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCC8427508.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2723..3301)
                     /gene="pgsA"
                     /locus_tag="MB219_001794"
     CDS             complement(2723..3301)
                     /gene="pgsA"
                     /locus_tag="MB219_001794"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCC8427509.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3510..4433)
                     /locus_tag="MB219_001795"
     CDS             complement(3510..4433)
                     /locus_tag="MB219_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427510.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5172..6458)
                     /locus_tag="MB219_001796"
     CDS             complement(5172..6458)
                     /locus_tag="MB219_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCC8427511.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6451..7719)
                     /locus_tag="MB219_001797"
     CDS             complement(6451..7719)
                     /locus_tag="MB219_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCC8427512.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7971..8432
                     /gene="mscL"
                     /locus_tag="MB219_001798"
     CDS             7971..8432
                     /gene="mscL"
                     /locus_tag="MB219_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCC8427513.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8491..8937)
                     /locus_tag="MB219_001799"
     CDS             complement(8491..8937)
                     /locus_tag="MB219_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427514.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9030..9275)
                     /locus_tag="MB219_001800"
     CDS             complement(9030..9275)
                     /locus_tag="MB219_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCC8427515.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         2572 a         2023 c         1659 g         3077 t
ORIGIN      
        1 acgatttctt tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca
       61 gccgtttttg tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt
      121 tcatcttctg ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa
      181 acttctgcca tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga
      241 ccaatccgat cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct
      301 actgccatgt ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact
      361 tcagcgattt tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta
      421 ccaacaatat ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt
      481 gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct
      541 actttttcac ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca
      601 gagagtttac gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc
      661 atttcgccgt cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata
      721 tctacagccc cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca
      781 ggctgtgaaa gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct
      841 aacgcatgct cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata
      901 gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt
      961 ccacgggggt aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt
     1021 gtagagattt gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc
     1081 ttttcaattt ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata
     1141 atcctcctta tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag
     1201 tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt
     1261 tttctcaaaa gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg
     1321 gcaaagtgat atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt
     1381 cctgcagatt gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa
     1441 tctgttcttg ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact
     1501 gttccatatt ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc
     1561 gagtaggtaa cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg
     1621 gcttggaagg cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc
     1681 agtagttcca ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc
     1741 cctactcgtt cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca
     1801 tcatttgttt tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc
     1861 gttggattga tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca
     1921 atcccataaa agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct
     1981 agtaaaggac gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga
     2041 aggatatacg catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt
     2101 ggtagaggaa ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt
     2161 cgatgggttg tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga
     2221 gacttaccga ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat
     2281 ccgccacata agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa
     2341 cgctgctgat tatctcctac aactgtttga taataaactt cgatacctag atcagctagc
     2401 tgttctgaca agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg
     2461 acagctatta tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt
     2521 tatatttttt tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct
     2581 tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa
     2641 aacatgaaaa gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca
     2701 caacctctcc actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga
     2761 agtaatccac acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta
     2821 ctggaatacc gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca
     2881 tttgtgtagc cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga
     2941 caagtagtaa acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc
     3001 aaccaggggc ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat
     3061 ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt
     3121 ttccatctaa ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg
     3181 tgacagccag tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa
     3241 ccaaaatgaa aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca
     3301 cactatcgac tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt
     3361 atttactttt ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga
     3421 attaacgaag aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag
     3481 taaacagatg cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca
     3541 ttttttggac tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg
     3601 atcgttgcat tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac
     3661 acggtactct gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact
     3721 agcacaccta accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg
     3781 tctgtgatat ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc
     3841 ttttcttcac ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt
     3901 tgctctgagg aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct
     3961 ccgattattg gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc
     4021 gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc
     4081 gattcttcct gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca
     4141 gggaccgatc ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct
     4201 tctcctacag cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat
     4261 tggtcaaaat catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc
     4321 tgtaattcat ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg
     4381 gtttcaaaag ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt
     4441 tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt
     4501 ccgaataaac attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata
     4561 taagaagctg ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac
     4621 ctctttctta aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa
     4681 tataaggaga tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca
     4741 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta
     4801 aaaacattaa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
     4861 tcacaacctc ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat
     4921 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     4981 ctgtcacaac ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga
     5041 gagcatgatt tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa
     5101 gctgattttg gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat
     5161 aaaatactgg tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat
     5221 gcttccccag ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca
     5281 aataaagttc gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa
     5341 tttaatgatt ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct
     5401 gacacttccg gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc
     5461 tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat
     5521 tcaaaaccaa agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa
     5581 gtattgccta aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct
     5641 gttccttctt gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta
     5701 atggctgctt ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt
     5761 acaatttctt gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc
     5821 aatttctcag gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac
     5881 gtccgatagc acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc
     5941 gcaatatcga tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc
     6001 cgccagttag ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg
     6061 tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc
     6121 ttttctacct gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca
     6181 ttggcggaag ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac
     6241 aacttatgtt ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca
     6301 tagggaataa attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg
     6361 tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa
     6421 agtgtttcat tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa
     6481 gaagattgcc tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct
     6541 ctccatacgt ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa
     6601 aaaagaagct tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag
     6661 cattgctttt gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa
     6721 ttgctcgtga atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac
     6781 actcatgaat cctcggaacg tatccacgct actagaggca taataagcta gactttcttt
     6841 ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac
     6901 cattagcgca aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag
     6961 ctttgcttga acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg
     7021 ggtataaaac agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc
     7081 ttcagctgct ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc
     7141 catcatttct tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac
     7201 tacgcccaac cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc
     7261 ataggaagca aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt
     7321 cttttccaag tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa
     7381 aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc
     7441 attcacaaaa ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa
     7501 tccaaagctt gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccggata
     7561 attcaaacta tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg
     7621 ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa
     7681 gacatgaaga ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca
     7741 gttgattgcc cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct
     7801 tgaaatttat aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta
     7861 tagcatagat gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat
     7921 tttttatttt tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag
     7981 aattcaaaga gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg
     8041 gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag
     8101 gttggtttgt cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg
     8161 actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta
     8221 catttctgat cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga
     8281 atttacgctc caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc
     8341 ttagtgacat acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag
     8401 agcaaacaaa tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat
     8461 tttcagtgtc ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc
     8521 gatatgtgga atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc
     8581 ataaaaagct tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt
     8641 ctggatcatg ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc
     8701 ctgatgaaca aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta
     8761 ggcaaggatc ttcccatcag tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc
     8821 atcaacttct tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac
     8881 gatcgcgtaa aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa
     8941 gcaaacgccc cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga
     9001 accttacgaa tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca
     9061 actgtccgag taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca
     9121 ggatccaaat caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct
     9181 gtttgacgct ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct
     9241 aaatctttct tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat
     9301 accacatatt tttaaaattt tcaagtttac c
//
LOCUS       DAHEYX010000112         9318 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHEYX010000112 DAHEYX010000000
VERSION     DAHEYX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.113"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(180..1547)
                     /gene="nhaC"
                     /locus_tag="MB219_001801"
     CDS             complement(180..1547)
                     /gene="nhaC"
                     /locus_tag="MB219_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCC8427516.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1656..3077)
                     /locus_tag="MB219_001802"
     CDS             complement(1656..3077)
                     /locus_tag="MB219_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8427517.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3320..5197)
                     /gene="tdc"
                     /locus_tag="MB219_001803"
     CDS             complement(3320..5197)
                     /gene="tdc"
                     /locus_tag="MB219_001803"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCC8427518.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5488..6744)
                     /locus_tag="MB219_001804"
     CDS             complement(5488..6744)
                     /locus_tag="MB219_001804"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCC8427519.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6856..7061)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7324..8625
                     /locus_tag="MB219_001805"
     CDS             7324..8625
                     /locus_tag="MB219_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCC8427520.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8694..9293)
                     /locus_tag="MB219_001806"
     CDS             complement(8694..9293)
                     /locus_tag="MB219_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8427521.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2816 a         1936 c         1662 g         2904 t
ORIGIN      
        1 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
       61 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt
      121 gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt
      181 caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca
      241 agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata
      301 atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct
      361 agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt
      421 tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag
      481 ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga
      541 acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa
      601 atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc
      661 tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc
      721 gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat
      781 gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat
      841 gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct
      901 agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat
      961 gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata
     1021 cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg
     1081 gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa
     1141 aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt
     1201 gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa
     1261 accataatcg tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa
     1321 agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc
     1381 tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg
     1441 atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac
     1501 acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta
     1561 tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa
     1621 tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct
     1681 acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg
     1741 accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca
     1801 aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt
     1861 ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc
     1921 aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt
     1981 gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata
     2041 acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat
     2101 ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca
     2161 gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt
     2221 ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt
     2281 gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc
     2341 aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct
     2401 ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca
     2461 gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag
     2521 tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg
     2581 aacatgatga ctactgggat atatacccct agccatacac cgatgttccc gccgactttt
     2641 gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct
     2701 aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt
     2761 cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca
     2821 acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct
     2881 tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa
     2941 actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc
     3001 cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt
     3061 acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct
     3121 ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag
     3181 tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag
     3241 gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt
     3301 tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt
     3361 tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt
     3421 ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt
     3481 tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt
     3541 ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca
     3601 cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt
     3661 tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa
     3721 cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat
     3781 gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga
     3841 catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat
     3901 aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag
     3961 agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat
     4021 agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct ttatacgcac
     4081 tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa
     4141 catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc
     4201 cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata
     4261 cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta
     4321 caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt
     4381 caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta
     4441 aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca
     4501 accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt
     4561 cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta
     4621 attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca
     4681 atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa
     4741 cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt
     4801 cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca
     4861 tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt
     4921 aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata
     4981 gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg
     5041 taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca
     5101 attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg
     5161 catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata
     5221 tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg
     5281 cctgattttt aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa
     5341 taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg
     5401 acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc
     5461 tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt
     5521 cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa
     5581 atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg
     5641 tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg
     5701 tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt
     5761 tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc
     5821 ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt
     5881 ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt
     5941 aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa
     6001 ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa
     6061 tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg
     6121 tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc
     6181 accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag
     6241 gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc
     6301 ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa
     6361 agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa
     6421 caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg
     6481 caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat
     6541 cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa
     6601 atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt
     6661 ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca
     6721 agttaattca tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag
     6781 gaacctgcta gcatgtgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt
     6841 cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga
     6901 tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc
     6961 tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac
     7021 attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat
     7081 catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt
     7141 ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa
     7201 ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat
     7261 tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg
     7321 tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt
     7381 ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct
     7441 gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact
     7501 caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa
     7561 gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa
     7621 gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg
     7681 atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg
     7741 gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta
     7801 gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt
     7861 gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa
     7921 tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa
     7981 aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc
     8041 gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc
     8101 ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat
     8161 gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac
     8221 ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa
     8281 gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca
     8341 aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa
     8401 aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct
     8461 tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc
     8521 gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc
     8581 aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat
     8641 gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc
     8701 ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt
     8761 tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag
     8821 aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca
     8881 agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt
     8941 tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga
     9001 taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca
     9061 atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc
     9121 agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca
     9181 gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct
     9241 gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga
     9301 gcctcccgta attttaaa
//
LOCUS       DAHEYX010000113         9242 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHEYX010000113 DAHEYX010000000
VERSION     DAHEYX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.114"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..970
                     /locus_tag="MB219_001807"
     CDS             <1..970
                     /locus_tag="MB219_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCC8427522.1"
                     /translation="IEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLVQRHDELYLVNR
                     TSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAENLYRNSLGADS
                     LKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFFKEYDYVFVCIG
                     DTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFNCLYTDSNSKNR
                     CSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKVNQNNMTNSTLF
                     SWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            963..1424
                     /locus_tag="MB219_001808"
     CDS             963..1424
                     /locus_tag="MB219_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCC8427523.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1437..2297
                     /locus_tag="MB219_001809"
     CDS             1437..2297
                     /locus_tag="MB219_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427524.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            2335..2724
                     /locus_tag="MB219_001810"
     CDS             2335..2724
                     /locus_tag="MB219_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCC8427525.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2708..5158
                     /locus_tag="MB219_001811"
     CDS             2708..5158
                     /locus_tag="MB219_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCC8427526.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            5163..7271
                     /locus_tag="MB219_001812"
     CDS             5163..7271
                     /locus_tag="MB219_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCC8427527.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            7268..8272
                     /locus_tag="MB219_001813"
     CDS             7268..8272
                     /locus_tag="MB219_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCC8427528.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            8291..9202
                     /locus_tag="MB219_001814"
     CDS             8291..9202
                     /locus_tag="MB219_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCC8427529.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
BASE COUNT         3056 a         1463 c         1974 g         2749 t
ORIGIN      
        1 aattgaagaa aagtcaaata atgtcataat tggatttctt tttacaaaac aatctggatc
       61 tataaaagat tataagggta cgcctaggac tgtattagtt cagcgtcatg atgaattata
      121 tttggtgaat agaacgagcg gagaaaacta tggattgggt gctaatatag ctattgttgg
      181 ggtagggtca gttggcagta aaattgctga aaatcttggt ccattaggtg tgagaaagat
      241 gacactcata gacaatgatt gttttcaagc tgaaaatttg tatcgaaatt cacttggtgc
      301 agatagttta aaaaatgccc gatctcagaa taattttttt aaagttgatt taatgcagga
      361 ggagctcgaa aaaaaattcc catacctttg tgttgaagtg gagtcaatga acgttttaga
      421 cttaattagt atgcaacctg gttttttcaa ggaatatgac tatgtttttg tttgcatagg
      481 agatacaatg gcaagcctgg ctttaaataa ttatttttcc aaaagtagtc aaaaagtttt
      541 ctattcttgg ctggagccat atggcattgg gggacacact ctatttataa attattctct
      601 aaatcaaaaa ggttgtttca attgtcttta tacagattca aactctaaaa ataggtgttc
      661 aaatagagct tctctggtaa aagaaggaca aatttttgaa aggactttag cgagttgccg
      721 ctcaaaattt ataccatata atgctattgc ctcgacggaa tgtgcaatta atgcaataaa
      781 acttttccat aaagtcaatc aaaataatat gaccaatagt actctttttt catggatagg
      841 agatgggagt acttttgaga atttaggata tcaatatagc gacagaaata cttttcttaa
      901 tggagtcaat aagtttgaag aaagattctt ttctaaatat tgcaaagtat gtggggagtt
      961 agatgattaa attttcgtgt gaccgtattg tcattgaagt aagggatgaa gttcttaaga
     1021 tatttgaaca atatcggcag ggaaaggatg acttgcctga agctggagga gttcttcatg
     1081 gaaaaagact taagaatggg agcataataa ttaccgatat aacaacacct caaataggag
     1141 atatacaaaa aagattactc tttaaaaaaa accgagaaat tcatcaacag ctttctaatg
     1201 agaaatggat agaatcaaat aagttttcta ttgcattggg agaatggcat actcatcctg
     1261 aagctgtacc aactccatca aatgttgata agaaaagctg gagactgaac gtgagcaagc
     1321 aacatgatga tagagtttat gtttttatta tcgttggatt ttctgatttg agaatgtggt
     1381 tactgtcaaa aaaatcagat attaggcagg ctataaaact ataaaggagt gattaggtga
     1441 aatcacaaaa gaatatttgg tggtgggggt ttttagtagg aaaggtaatg ataccagccc
     1501 ttctcttaat ttctctaccg atgattactt ttttctttac caataaaaaa atgagtaaag
     1561 aagccgtgat ttttttcttt ggagaccaaa aagctacctt tattgaaacg ttagtaacat
     1621 ctcttcagct taatttgaat tacatttttt caattattat tgttatatcg ttattgaatt
     1681 tttttaaaaa aagaaattca ggaaaggtgt ttaactctaa tggaaatgtg tactacaact
     1741 atttttattt tgtattttgg gtagcagcaa ctttacttgg ttatgataaa attcagattg
     1801 caggaattcc tatacacatg caatataagt tggttttgag tggaattttt tctgaggtgt
     1861 tgccagatat atacgatgat cattacgaca gtgatggaac atgtaaagta agcattgaaa
     1921 aagaaaattt cgatgacatc gatggatatg actctgtgaa cttactgatt attgatactt
     1981 acgatataaa aatgagtgaa ttatctatgg aaaatcaaac atatcctacg ataattgtta
     2041 gagggaattc aatagatgga gttcgtaaag ttaatagatc tttaatcttg gaaataaaaa
     2101 aaacaatgga tgaaattcaa aaaagtgact ttaaaaaagt atttgttgct tcaacatcta
     2161 atccaaaaaa tagtatcaat attattaatt catcgtttag atttttcggt cgatcacgta
     2221 ggtttaaact ttatgttctt caaaaggatt atgcaagcaa tggaaagtat agtaagaaat
     2281 atagaatttt tatttaagaa ggaatttttc cttcttttta ttttggagga aactatgaaa
     2341 aaaataaaaa gctacaccag tatttgggca gttgaaaaag tcatttatgc gattaatgac
     2401 tttcagctgc cttttcctgt gacctttaac cagatggctt ggtttgtttt atccttgatg
     2461 tttgtcattg tatttgccca tgtgcctccg ctatccatga tagagggggc ttttttaaaa
     2521 tacttgggca ttccgatagc ggtcacttgg ttcatgtcgc aaaagacctt tgacggtaag
     2581 aaaccattgg gatttttacg atcctttatc agctatcact tacggtttaa ggtcactttt
     2641 gcaggaaaga aagttaaaga acagaagaaa cgatgggacg aaccaatcac cttggtaagg
     2701 agtgtgaatt atgtacccga ttaagtatat tgaaaataat cttgtcttta accaagaagg
     2761 cgagtgtttt gcttactatg aattagtgcc ttacaattac tcctttttgt cacctgaaca
     2821 gaagtatcaa gtacatgata actttcgcca attaattgcg caaaatcgtg aaggtaagat
     2881 ccatgcctta caaattgcca cagaaagtag tattcgtgcc acacaagaac gttcgaaaaa
     2941 ggaaattacc ggtagactca aagaagtagc aaagcaacga attgatttac agacagatgc
     3001 attagtatcc atgattggcg atagtcagat tgattatcgc ttctttattg gctttaaact
     3061 gatttcgaca gatgaagagg tcaatctaaa gaatctcagg aaatctttct tttctggact
     3121 tcaagaattt gtctatggag tgaatcatca tttgatgggt gattttgttt ccttatccaa
     3181 tgaagaaatt cgtcgctata ccaagcttga aaaactaatg gaaagtaaat tggctcgaag
     3241 atttaaagtc aaacgagtaa cgccgagtga cttgacgtat ttaattgaac atatctatgg
     3301 tgaaaaaggc acaccgtttg aagaatatga attccagcta ccaaagaaaa agctgaaatc
     3361 agaaacgctg gtgaaacgtt acgacttatt acgtcccact cgttgtttga ttgaagaaaa
     3421 gcctcggtac ttacggatgg aacatgaaaa tcatgagtct tatgtagcgt acttaacaat
     3481 taataccatt gtaggggaaa tggagtttcc ttcctcggaa cttttctatt accagcaaca
     3541 acaatttacg tttccgattg atacttcaat gaatgtggaa attgtgacga ataaaaaggc
     3601 cctagctacc gttcgtaata agaaaaagga attaaaagat ttagacaacc atgcctatca
     3661 atctgataat gaaacgaact ccaatgtatt agatgctttg gattccgtgg atgaattgga
     3721 aaccaccttg gatcagtcga aagaatccat gtataaacta agctatgtgg ttcgagtgag
     3781 tgctaaatca gtggatgaat taaaacgccg ctgtgatgaa gtgctcgatt tttatgacga
     3841 taccaatgta aaactagtcc ggccctttgg cgatatgatg ggtctacaca aagaattctt
     3901 accctcaagt aagcgataca tgaatgacta tattcaatat gttacgtctg actttttggc
     3961 gggacttggt tttggggcta ctcaaatgct aggagaatta gaaggcattt actttggcta
     4021 taacgtagat acggggcgca atgtttacct gaaaccggca ttggcatcac aaggggtcaa
     4081 aggctcagta accaatgctt tggcatctgc tttccttggt tcgttaggtg gggggaaatc
     4141 ttttagcaac aatctcttag tctattatgc cgtccttttt ggtgggcaag ctgtgattgt
     4201 cgatccaaaa ggagaaagag gcggttggaa agaaacgttg ccagaaattg cggatgaaat
     4261 taatattctt aacttgacca gtaagccaga aaatcaaggc ttacttgatc cctatgtgat
     4321 tatgaagaaa aagaaagact cagaaagttt ggcgattgat atcctgacct ttttaacagg
     4381 gatttccagt cgggatgggg agaagttccc tgtgttacgc aaagccattc gaacggttac
     4441 ccaaagtgat cagcgaggat tattacaagt aatcgttgaa ttgcgtaaag aggactcacc
     4501 agtcgcggaa aatatcgcgg atcacatcga gtcctttacc gattatgatt ttgcccattt
     4561 gcttttctca gatggttcga ttacccagtc gattagttta gataagcagt taaatatcat
     4621 tcaagtagcg gatttggttt taccagatgc agaaaccact tttgaagaat ataccaccat
     4681 ggaattgctg agtgtttcca tgctaattgt tatttccact tttgccttgg actttatcca
     4741 tagcaaccga gaaattttca agattgtgga cctggatgag gcttggagtt ttcttcaagt
     4801 agctcaagga aaaacgttat cgaataaatt agtgcgtgct ggtcgggcca tgaatgccgg
     4861 tgtttacttc gtcacgcaaa acagtgatga cttgttggat gaacgtttga agaataatat
     4921 tgggctaaag tttgcatttc gcagtaccga tattcatgaa atcaagaaaa ccttggaatt
     4981 ctttggatta gataaagaag atgaaagtaa tcaaaaatgc atgcgagatt tggaaaatgg
     5041 gcaatgcttg atgcaggatt tgtatggtcg agtaggtgtg gtccaagttc atccagtctt
     5101 tgaagaactt ttccatgctt ttgatacccg tccgccagtt caagagagaa tggagtgagg
     5161 agatgaaaaa gaaacttttt cgtatctttg gaattgtttt aatcagtttg gtcactctga
     5221 taatactttt gagcttagtt ggaacggtag ctgaggcaac aggtctggtt gatgatacag
     5281 ttggagccgg aaatctttat tcccaatatt cacttggtaa ctatcagttg gatttctttg
     5341 tggatagttc gtgggattgg ctgccttgga attggggtga tggcctaggg aagagcgtaa
     5401 tgtatggact ttatgccatt actaatttta tttggacagt tagtctctac ttatcaaatg
     5461 ccacgggcta cgtggttcaa gaagcctata agctggattt catttcagat acggctgaaa
     5521 gtattgggaa gaatatccaa accttagccg gcattaccga aaatggactc cagacctcgg
     5581 gcttttactt tgggtttcta ttattgatga ttttagctct aggagtttat gtggcgtata
     5641 caggattatt aaaacgagaa actaccaaag cagtccgggc tgtcattaat tttgtgatga
     5701 tttttctact ttcgggttcg ttcattgcgt atgcgcccac ctatatcaca aaaatcaatg
     5761 attttagctc agatgtcagc gaagcggctc ttagccttgg aaccgagatt gtcgtgccga
     5821 attcagaaag ccaaggaaag gacagtgtgg atttaatccg agatagcttg ttttccattc
     5881 aagtccaaca accgtggtta ctcttgcaat ttgatgattc caatatagaa gaagttggcg
     5941 aagatcgtgt taacaaaatc ctgtctgtaa gtccagatga aaataaaggc aaggatcggg
     6001 aagaagcggt caaagcggag attgaagata atgacaatgc gaatctcagt attaccaaga
     6061 ccatgaaccg cttagggacc gtggtctttt tggtgttgtt caatattggc atatccttct
     6121 ttgtctttct cctaacaggg attatgttgt tctcgcaaat tctctttatt atctttgcga
     6181 tgtttttgcc gattagtttc ttattgtcca tgttgccgac ttatgagagt ttgggcaaga
     6241 aggcgattat tcgcttgttc aatacgatta tgatgcgagc aggagttact ttggtgatta
     6301 cgactgcatt tagcatctcc acgatgtttt tcaatatttc ggcaacctat ccatttttca
     6361 tggttgcttt tctgcaaatt gtgacctttg ccggtatcta tttcaaattg ggcgacatta
     6421 tgaatatgtt caatctccag agtaatgata gccaatcaat gggaagacgg gtcatgcgaa
     6481 aaccgcaaat gttaatgaat cgaacattgc ggcaactcaa tcgaaatgtg ggacggactt
     6541 tggcttttgg tggtggtgca gcagttggaa ataagttggc aaaggaacaa tcaaaatcca
     6601 aatttaagcc atccggttct tctctacgaa agaattcacg attaccgaac gatcacgaga
     6661 taccatctga cttgaataag gaaaagccaa ttccaaataa taagaaacaa tcccgaatga
     6721 atttggccgg gagaaaagtc ggcaaggttc tggataccca agctttggtg aaagataaga
     6781 ccaaacaagt aaaggatgga gtacggaata cgccaaccaa cttgaaatac aacttccata
     6841 aaggaattga aaaaacgaaa aaagcaccgg aagagtttaa gcgaggactg gtccaagaaa
     6901 aagcaaatcg aggagagttg cgagaaaaac aacaacaacg tagagacgaa aaaatagctg
     6961 aaaagcgcaa agtattgaac gaaataggga atcctcatgg gaaaagaagg gaaaatgttc
     7021 cgataaaagc aaaggtccaa tctcaaaagg atagagcacc aaagcggatt gtgagagcaa
     7081 attcgaatcc cgaaatcaaa cgaaaattcg ctagccaaga aatcatttca aaaggaataa
     7141 accaatcctc aagtaagaaa aattcttttc aacaagtgaa acggcggtct actctgtcca
     7201 aacgaaccaa tcgaaaagta caaaaaagtt tgaaccctca tcccaaacca aggtcaggtg
     7261 agaaaaaatg aacgtaaaga aattaagtgc catttcgtta actttcctaa caatctcttt
     7321 tatgggtatc ttgatgtgtg tgggcttatt gtttggtgaa gacagtgaag gaagtagtag
     7381 ttcaggtgct cctcctgggg ggagtagtgt ctcagaagag gttttgaaac accgtgcaat
     7441 ggtggaaaaa tatgccaaag aatcggatat aacagagtat gttccgattc ttttagcgat
     7501 tattcaagtg gaatctggtg gcacaatgga agatgtgatg caaagttctg agtcagccgg
     7561 tttaccaccg aattcattaa gtaccgaggc atccatcaaa caaggatgtc tctattttgc
     7621 gtctttggtt aagcgttcca aagaattagg ttgtgatcaa gatagtatca ttcaggccta
     7681 taactttggc ggtggttatt tggactatgt ggcaaaaaac gggaagaagc atagttttgc
     7741 tttagctgaa tctttttcaa aggaaaaatc ttgcggtcaa aaaatcgatt accctaatcc
     7801 catcgcgaat aaggaaaatg gtggatggcg ctataactat ggcaatatgt tttattgctt
     7861 attggtgaag caatacctaa ctacaacaca gtttgacgat aaaacagtcc aagggatttt
     7921 tgacgaggcg tttaagtatg aaggaacggc ttatgtattt ggtgggtcaa gacccgaaac
     7981 aggatttgat tgtagtggct tgactcaatg gtgttatgcc aaagttggtc tgaaacttcc
     8041 tcgggtggct caagatcagt atgatgccat gacccatatt gacctaaaag atgccaaacc
     8101 aggggatttg gttttctttc attcgactta tgatgccggt acttatgtga cccatgtcgg
     8161 tatttatgca ggggagaatc gcatgtttca tgcaggagat ccagtaggct ggacgaatct
     8221 aacagaaagc tattggcagc agcatcttat cggtgctgga cggtacaaat aagagaaaag
     8281 aggaaaaaga atgaagataa aaattgaacg gaatcaaaag gaaaagagcc taaaaaagaa
     8341 aaaagaacga gtcgtatccg tgggtaagca tcgaaaaatg gtgcttgccc tttggctttt
     8401 attatcttgt agtcttactt ttggcgttta taaaaatttc accgccattg atcaacatac
     8461 aactcatgaa aaagtggtta ttaaagagaa agtagtgaat acttctggta ttgaaaactt
     8521 tacgaaggat ttttcgaaag aatatttttc gtggaaaaat aacaaagaag caattgaaaa
     8581 gagaatgaac aacctcgaac agtaccttac agaagaaggt ttcgctttaa gtcaagatat
     8641 ggttcgagca gatattccca ctaattccga agttcagtca gtaaaagtct tagatgttga
     8701 aaagagctcg gaggaatttg tcgtttcatt tttagtggag caaaagatta cagagggaaa
     8761 aaaggcacaa tcgatttctt ctgcataccg agtgacgatc tttgaagatg aaaacaggaa
     8821 ttatatagta acgagtttgc caactatgat tgcaaaacct gatagggcaa aatacaagac
     8881 aaaacaagta gaaaatgatt cgaaaattga tgccaaaact acagaagaaa tcgctgagtt
     8941 tttggaaacg ttctttaaat tatatccatc agcttctgaa aaagaattgg agtattatgt
     9001 tgaaaatgat gcgatgcgac caatcaatat tgacttaaag ttttcgaagt tagaggatcc
     9061 agtttaccta caaaacggaa aaaaaattct agtgagtaca aagataaaat accttgataa
     9121 tgtatcaaaa accactagct actttcagta caaattaata ttacaaaaaa atggaaactg
     9181 gaaaatcatt gatgttaaat aataatgact caatctaaat ttatgagaag ttataagtag
     9241 tt
//
LOCUS       DAHEYX010000114         9196 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHEYX010000114 DAHEYX010000000
VERSION     DAHEYX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.115"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            3..1457
                     /locus_tag="MB219_001815"
     CDS             3..1457
                     /locus_tag="MB219_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCC8427530.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1533..2693
                     /locus_tag="MB219_001816"
     CDS             1533..2693
                     /locus_tag="MB219_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCC8427531.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2806..3813
                     /locus_tag="MB219_001817"
     CDS             2806..3813
                     /locus_tag="MB219_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCC8427532.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3837..4862
                     /gene="iolG"
                     /locus_tag="MB219_001818"
     CDS             3837..4862
                     /gene="iolG"
                     /locus_tag="MB219_001818"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HCC8427533.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4879..5769
                     /gene="iolE"
                     /locus_tag="MB219_001819"
     CDS             4879..5769
                     /gene="iolE"
                     /locus_tag="MB219_001819"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HCC8427534.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5792..7387
                     /locus_tag="MB219_001820"
     CDS             5792..7387
                     /locus_tag="MB219_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCC8427535.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7403..7717
                     /locus_tag="MB219_001821"
     CDS             7403..7717
                     /locus_tag="MB219_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCC8427536.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8178..8903
                     /locus_tag="MB219_001822"
     CDS             8178..8903
                     /locus_tag="MB219_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCC8427537.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9036..>9196
                     /locus_tag="MB219_001823"
     CDS             9036..>9196
                     /locus_tag="MB219_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCC8427538.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLP"
BASE COUNT         2809 a         1662 c         2150 g         2575 t
ORIGIN      
        1 aaatgacaag atttttaaga aattatattg atggtcgttg ggtcgctagt cgctcaacag
       61 agtttgaaga agtaatgaat ccggctacag aagaagcgct ggcgaatgta ccgttgtcta
      121 gcaaagcaga gacagccgaa gcagttaaag ctgccgatgc tgcgtttcaa acttggaaaa
      181 aagttcctgt tcctgttcgc ggacgttatc tgttcaagct gcatcagctt ttaacagatc
      241 ataaaagtga attggcgcgt ttgatcacgc tggaaaatgg gaaatcattg tccgagtcac
      301 tgggagaagt tcaacgagga atcgaaaatg tggagcacgc agcgggaatc accaatttaa
      361 tgatgggaga ttctttatca acagcggcga ctgatattga gatcacgaat tatcgttacc
      421 caatcggagt aatcggaggg atcacaccgt ttaattttcc aatgatggtg cccttctgga
      481 tcttcccgat ggctctggcg gcaggaaata cggtggtgct gaagccttca gaaaaaacac
      541 cattgttgat ggagaaaatt gttgagctgg ttgaaaaagc aggctttcct agcggtgttt
      601 ttaatgttgt ctatggtgcc cacgatgttg tcaacacatt gctgcaagat ccactggtaa
      661 aaggcatcag ctttgtagga tcaaaaaatg tcggtgaata cgtttaccgt gaaggaacaa
      721 aaaatctaaa acgggtacaa gcgttgactg gagcgaaaaa tcatgttgtt gtgctgaaag
      781 atgccaatat tgatcgggca gtcaaggacc ttattggcgg agcttttggt tctgctggtg
      841 aacgctgcat ggcagcctcc gtcattgttt tggaagagga aatcgcggat cagtttgttg
      901 aaaaatttaa ggctgcggca aaagcgatca agatcggcaa tggcttggac gaaggggtct
      961 ttttaggacc agtgattcga aaagatgcac agcagcggac ctttgactat atccaaaaag
     1021 gggtcgatga aggagcgaaa ctggtactag atggtcgcga aaacattcca gaaaaaggct
     1081 attttgttgg accaacgatt tttgataatg tcacgatgga tatgaagatc tggcaggatg
     1141 agatttttgc cccggtcgca tcaatcgtgc gggtaaaaga tttgatggca gccgttgagc
     1201 tgacgaatga atctgaattt gcgaatggtt cgtgtctgta taccaacaac gcatcagcga
     1261 ttcgctactt cagagaaaat gttgatgctg ggatgctggg ggtcaatctt ggggtgcctg
     1321 caccgatggc ttggtttgcc ttttcaggct ggaagcactc tttctttggt gatttgcatg
     1381 ccaatggcaa agacagtgtt gagttttata cccatcggaa agttgttaca gcacgttatg
     1441 cgcaaagtga gatttaaaga tttgcttcga agctctgtta aagagcttcg aggattctgt
     1501 tataggctat aagccagaga gaggtttttt ctgtgaatat tcaactgaaa atgcctgcca
     1561 atttaagaat tggcggcgga acaatccaat ttcttatgga agaatcagaa caactaggca
     1621 acaagccgtt aatcattagt gatacggtta tggaacagac cgggcatctt caaaagatca
     1681 aggagctgtt gggaaaaaca gatgatcaat tagcggagta tacagacatt caagccgaac
     1741 cgacgcaaga aaatgtttat gatgcgctga agctttttca gaagcatcaa tgtgatcata
     1801 tcattgcttt tggtggcggc agctgtctgg atacagccaa ggtggtagcc gttttggctg
     1861 ctaatcaatt aagtctcgaa gcgattcatg caagaaattt tgatcagctg caagcgggca
     1921 ttccgctgat tgcagtgtca acaactgctg gaactggttc ggaagtaacc gatgtggcag
     1981 ttattaccaa tacaaaaaca gcagtcaaaa tgatgatgaa ggatctcgtt ttcatgccac
     2041 agatctcaat cgttgactct gattttacga aaactgtacc accaaaagta accgcggcaa
     2101 cgggagtcga tgcgctatgt cacgctttgg aatcttatgt ttcagtcaaa gccaatgaag
     2161 taacgcaact attttcactg gaagcgatga agaaaatttt gaacacgctt tctcaggcat
     2221 atgaaaatag cgacgacact gaagccagag aacaaatgag cttagcttca acggaggcgg
     2281 ggattgcctt ttcgaatgcg tcagtcacat tgattcatgg aatgagtcga ccggtggggg
     2341 cactttttca cattcctcat gggatatcaa atgcgatgtt attgccggtc ttttttgaat
     2401 tctcaaagga tgctattcag actgagttag cgacgatcgc cagatttatt tatccggcag
     2461 ccgaagattt atcgctggaa gaggctgcgg attatctgat taaaaaaatc caagagctct
     2521 gtgagcagct gtcgatccca acgattcgag attatggtat tgatcagcaa gcgtttatgg
     2581 cagccttaga caaaatggcc gcggatgcgt tgaccagcgg aagtccgcaa aataataaaa
     2641 aggtgcctag tcatagtcaa attaaggaat tatacgtaca agcttatggg tagtaaaagg
     2701 cggagcaaag agagggctct accttttaag aataaaagct acacaggaat aattttgtaa
     2761 gcgcatacta tgtgtgctta aacaacagaa cagggggata agtaattgag agtaaaagta
     2821 ggcgttatcg gaacaggtgc aatcggcatc gagcatatcg acaggatcaa tcagaaaaca
     2881 caaggggctt atgtgacagc agtctcagat atcaatgtaa aagctgctaa agaggttgcc
     2941 aaacgtgtcg atgctaaatt ctttgaaacc ggagaagcat tgatcgcttc tgaagatgtg
     3001 gatgtcgttg ttgtgacttc ttgggacccg acccatgagc agtatgtgtt agaagcaatc
     3061 aagcatggga aatatgtttt ctgcgaaaag ccattagcga tggatgcagc aggctgccgc
     3121 cggatcgttg atgcggaagt tgcttatggg aagaaattgg ttcaggttgg cttcatgcgc
     3181 cgctatgacc gcggctacat tgaactgaaa gaagcaatcg aatcgaagca atacggagaa
     3241 gcattaatga tgcattgtgc ccatagaaat cctagcgcag atgaaaacta cacaacaccg
     3301 atggcgatca gcaacaccgc gattcatgag attgatgtgc ttcgctggtt gctaaatgaa
     3361 gattatgaat ctgttcaaat gatcttgccg aaaaaaacca gcaactccca tgccgatttg
     3421 catgatccgc agctgctgat ctttcaaaca aaatccggtg taacgattga tttggaaatc
     3481 tttgtgaatt gccgctttgg ctacgatatt aaatgtgatg tggttttcga aacggcagaa
     3541 gtaggaatga cggacccagc ctatacacga attaaatcgg aagaaaaaaa ttacacacct
     3601 gtttcaccag attggcagac ccgctttttg gatgcctatg attatgaatt caagctgtgg
     3661 atcgattcga tcaaaaatga tgcggttgtc ggtcctaccg cttgggatgg gtatgtagca
     3721 gcgatcacca tgatggcttg tcatgagtct cgtgaaacaa aaacaaaagt aatgattcaa
     3781 atggatgaac gaccagcact atacaatgag taattctcag aaaacggagg tatttcatga
     3841 acaacattag agtaggcatc gtcggtttag gaagattagg aaaagtacat gcacaaaatt
     3901 tagcaacagg tgtcagttgt tgtgagctta cggctgcgtg cagtttagtt gaaacagaat
     3961 tagcattcgc acagaagaaa ttaggcgtca agcatacttt ttccagctat gaagaaatgg
     4021 tcaaaagttc cgtgatcgat gcagtcttta ttgtttcacc atcaggcttt cactgtgacc
     4081 aagtccgctt ggcgatggaa aatggcaagc atgtctttac tgaaaaacca ttaggccttg
     4141 aagttgaaga tattaaaaag acccaaaaag tcatcgaaga gcatcctgaa caggtcttca
     4201 tgttaggctt catgcgccgc tatgatgagt cgtatcagta tgcgaagaaa atggtagaag
     4261 atggcgaaat cggcgaaatc acactgattc gttgttatgg gatcgatccg agcgaaggct
     4321 tggaaagctt tgtcaaattt gccatgaata acgacagcgg cggcttgttc ttagatatgg
     4381 cgatccatga tattgatttg gttcgctggt ttaccggcca agaagtcgat caagtgtggg
     4441 cgattgggaa aaatgcggcg tatccagaat tggatcaggc tggtgagctt gaaactggag
     4501 ccgcaatgat gagcctgagc gataaaacaa tagctttact agttgccggc agaaacgcag
     4561 cccatggcta tcatgtcgaa acagagatca tgggaaccaa aggaatgctg cgggtagcgc
     4621 aagcgcctga gaaaaatctt gtgacgatca tgaacgatca agggatcatc cgtccatgtt
     4681 cacaaaactt cccagaacga ttcagagaag ccttcattcg tgaagcccaa gaatttgtct
     4741 catgtattca agaaaaacgt caaccagcgg ttaatgcaga agatggattg caatcaacta
     4801 agatcgcgtt ggcttgtcaa gaatcattcg atacgaagag tttagtacaa ctaaaaaaat
     4861 aagcaggagt gtgagaatat gaccaaacaa attcaattag ccatcgcacc aatcgcgtgg
     4921 accaatgatg atatgcccga gcttggtagt gaaaatacct ttgaacaatg tatcagtgaa
     4981 atggcccttg ctgggttcac cggaacagag attggcaaca aatatccaaa agatccagag
     5041 gtgttggaac aatatttgaa tattcgcggc ttaaaggttg ccagtgcatg gttcagtgcg
     5101 tttctgacaa ctaaaccttt tgaagaaact gccgaagcct tcaaagcaca tcgggatttc
     5161 ttacatgcga tgggcgctaa agtcatcgta gtagctgagc agggtcatag tatccaaggg
     5221 attatgacag cgccagtctt tgatgataaa ccagttttca ctgatgaaga gtggaaaaaa
     5281 ctggcggaag gattagaaaa attaggggat ttggcccacg aaaaggatat ggagatcgtt
     5341 taccatcatc acatgggtac cggcgttcaa acgacggaag aaatcgatcg tttaatggaa
     5401 atgactgatc cagacaaagt taaattattg ttcgatacgg gacatttggt cttttctggt
     5461 gaaaatccaa tcgcggttta caaccgtcat caagatcgta tcaaacacat ccatttcaaa
     5521 gatattcgtc aagaaacggc tgaagaagta gaaaaggaaa aagacagctt cttaaacggc
     5581 gtgaagaaag gcgtcttcac ggttcctggt gatgggatga ttgatttcac tccaatctgg
     5641 acagcaatcc atgccagcga ctatgaaggc tggatcgtag ttgaagcaga acaggacccg
     5701 gctaaagcca atccatttga atatgcagtc aaagccagaa gctacattcg agaggtcacc
     5761 ggtctttaat ctattgaaaa ggagctagac gatgaatctt ttttctataa tcagtttcct
     5821 agtgattgtc atctgtgtat ggttatttgc ctattctaga tccagaagtg tcgatacttc
     5881 tggatcagaa gggttcttca tgggagggcg gagcttaact gctttgccaa tcgccggcac
     5941 gatcatcatg acgaacctgt ccaccgaaca aatagtcgga caaaatggtc aaagttatca
     6001 tgcagggatg gaagtaatgg cctgggaagt aactgctacg attgcgatcg tagctttggc
     6061 tgtgatattc ttgccaaaat attttaaata cggcatcaat acaatctcag atttcatcga
     6121 gattcgttat gatacggtta ccaaacgaat tatttcgatc ttatttattg tgacctatat
     6181 gacttctttc ttacccgtgg ttctttattc tggatcgctg gtgttcaata agatcttcca
     6241 tattgatgag ctgctgggcg tccagccgat cgtcgcgatc ataattgtag cgatggcaat
     6301 tggtattgtc ggcatcctct atctgctgat cggcggcttg tcgttaagtg ccaatagtga
     6361 ctccatttat ggagtcggtc tgctggcttg tggtctgctg gtcccaattt taggcttaat
     6421 gaagcttggt gacggcagct ttgttggcgg gatcgaacac gttgtggtaa acacgccatg
     6481 gctgcttaac tctgtgggat cgatcgactc ggctgttgtt ccatggccga cactatttac
     6541 agggatgctg tttaacaatc tgtatttctg gtgtaccaat caaatgatcg tccaaaaagc
     6601 attgtccggc aagaatttag ctgaggcaca aaagggcgct ttcctagtcg gcgtcttcaa
     6661 aatcttaggt gcgttattcc ttgtctttcc agggatcgtc gctcgcaata tttttggcga
     6721 tagtttaatg tcaaatccag ataacgcgta tcctttttta gtcagtgaag tattgcctaa
     6781 aatgatcttt ggtattttcg ccgctgttat ctttggcgcg atcttatcat catttgcggg
     6841 agctttgaac gcgacatcga ccttgttttc actggatttt tataaaccgc ttatcaataa
     6901 gaaagcaaat gataagcaaa tcgctcgtgc tggtaaaaca gtaacggtcg ttgtggggat
     6961 tatctccatt atcgtagcgc cattcatttc ttttgcaccg gctggattgt atcagttcgt
     7021 tcaagaattc aatggactat acaacatgcc gctgctcgtg atcattctct ttgcctttta
     7081 ttcaaaaaaa gcaactgctt ttggggcaaa aacgacgatt gccgtgcata tcgtcttgta
     7141 tgcactatca aaaatgcttt tccaaaatat ccatttcctt tatgttttga cggcattatt
     7201 cgttctggac attatcattt tgatggtggt ttccaaattg aaacctgatg gagaatttgt
     7261 gttgaactca ttcgatacaa aggtcgatat cgagccttgg aaatacacca aggtgactgc
     7321 tatagcattg gtagtcattg ttattttaac ttatgtggca ttttcaccaa ttggctttgc
     7381 aggctaaaaa caggagggta ttatgcataa ggatctattg attatttgcg agaccggaat
     7441 cagtgcgtcg ctgctcgtat caaaaatgct tgaaacgatc agagccaaaa atctatcgta
     7501 tgagatagat tatgcaacga tccgaaacgt tgaagaaaaa ctgaattata aagactatga
     7561 cgttttactt ttaacgccgc aggtcgcacg ttctgaggaa aaagtaaaag aaatcatcaa
     7621 aaaggaaaaa tgtaccgctc agatcgttta tatcaaacaa gaagattttc gttatatgaa
     7681 tattgaaaac atcattgcaa gcatcgaaga agattaacga gaatctttga ttattgagtg
     7741 tcgaggaggt ggtatatgaa aagatggcat gttcgccccg atcaccccaa tatgaagggc
     7801 ggacttatta gaaataccca atcttgtatt tattcaatag ggtaagcgga cctcaatagc
     7861 cagctcatcc ccaaaaagtc gaatggagac acttaaatga gtgtacctat tcgacttttt
     7921 gagactctaa gtcaaatcgt gttgttctca ttaaatcact gaaaggaaat gcttcggcgt
     7981 ttcctttttt ctttttgttt ttcttcgtgt tttgaaaatt acttactatt tactatacga
     8041 gtagtttcaa ataattttga catcctaatc ttcaacaaca gaaaaaaatc taaattataa
     8101 aatttagggt tgactaaaat ataatttttg tataccatat gatcgtgata atcattatca
     8161 tttagaagga gaaagttatg ttagaagtga aaaagcttaa tgtttgttat aacgattttt
     8221 tagcattgaa cgagatttct ttaaaaatac aagaaggtgc tttaacaggt attattggtc
     8281 caaatggtgc tggaaaatct acattgctta aagcaatgtt aaatattatc cctcatcagg
     8341 gagaggtctc cattaataat gaagatatca ataaaaaact ggtgaagatt gcatatgtag
     8401 agcaaaaagc tgatattgat tttacttttc caataaaagt aaaagaatgt gtatctatgg
     8461 gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt
     8521 caaaggcttt ggaaaaggtc gatatgggaa aatattctaa tcatcaaata ggagaattat
     8581 ctggaggaca atttcaacgt gtacttttag caagatgtct tgctcaaaat gcggatttca
     8641 tatttttaga tgaaccattt gtagggatag acttagttag tgaacgaata attatggata
     8701 cgttaaaaga attgaaacat cagggaaaaa cagtgctaat tgtgcatcat gatttaagca
     8761 aggtaaagaa gtattttgat aatattattc ttttgaatcg aaatctaatt gttcacggaa
     8821 gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc ctatggagat actatattta
     8881 ttggagaggg gggaacggct tagcctctca atccaataaa tatattatct caaaagaaat
     8941 gagttaatac caaattaaaa taacgatggt tcatagggtt atccaatcaa aaccggaagg
     9001 ttaaaaccct aactctaata tacaagggga ataatatgct tcaaaatttt attaatggtc
     9061 tgtatgactt tcacttttta cagaatgctc taattacatc tgtagttata gggattgttt
     9121 ctggaacagt aggttgtttt attattttaa gaggaatgtc tcttatgggt gatgctattt
     9181 ctcatgctgt tttgcc
//
LOCUS       DAHEYX010000115         9061 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHEYX010000115 DAHEYX010000000
VERSION     DAHEYX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.116"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..815
                     /locus_tag="MB219_001824"
     CDS             <1..815
                     /locus_tag="MB219_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCC8427539.1"
                     /translation="LLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTENPGKFIN
                     VQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIVNKDIGV
                     KDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGETAVQAQ
                     EQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWTIWAYEH
                     MYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE"
     gene            930..1850
                     /gene="pstC"
                     /locus_tag="MB219_001825"
     CDS             930..1850
                     /gene="pstC"
                     /locus_tag="MB219_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCC8427540.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1850..2734
                     /gene="pstA"
                     /locus_tag="MB219_001826"
     CDS             1850..2734
                     /gene="pstA"
                     /locus_tag="MB219_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCC8427541.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2749..3555
                     /gene="pstB"
                     /locus_tag="MB219_001827"
     CDS             2749..3555
                     /gene="pstB"
                     /locus_tag="MB219_001827"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCC8427542.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3571..4329
                     /locus_tag="MB219_001828"
     CDS             3571..4329
                     /locus_tag="MB219_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCC8427543.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4342..5019
                     /gene="phoU"
                     /locus_tag="MB219_001829"
     CDS             4342..5019
                     /gene="phoU"
                     /locus_tag="MB219_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCC8427544.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5257..6288
                     /locus_tag="MB219_001830"
     CDS             5257..6288
                     /locus_tag="MB219_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCC8427545.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6285..7052
                     /locus_tag="MB219_001831"
     CDS             6285..7052
                     /locus_tag="MB219_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCC8427546.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7152..8720
                     /gene="liaX"
                     /locus_tag="MB219_001832"
     CDS             7152..8720
                     /gene="liaX"
                     /locus_tag="MB219_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCC8427547.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
BASE COUNT         2933 a         1556 c         1968 g         2604 t
ORIGIN      
        1 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
       61 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      121 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      181 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      241 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      301 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      361 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      421 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      481 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      541 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      601 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      661 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      721 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      781 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
      841 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
      901 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
      961 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1021 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1081 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1141 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1201 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1261 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1321 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1381 gaagtatttt tggcgggact gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1441 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1501 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1561 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1621 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggtctta
     1681 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1741 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1801 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     1861 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     1921 tttagcagct ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt
     1981 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2041 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2101 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2161 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2221 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2281 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2341 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2401 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2461 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2521 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2581 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2641 cgcttctgcc gtgttgatta ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2701 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2761 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     2821 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     2881 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     2941 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3001 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3061 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3121 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3181 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3241 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3301 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3361 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3421 caagcagctc gtatcagcga ctatactgcc tttttctatt taggaaaagt aatggaatat
     3481 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3541 ggtcattttg gttaagacgg aggcctcaac atgacaaaag agattatttc atctaaagat
     3601 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa
     3661 ggggaaatca ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct
     3721 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3781 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     3841 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     3901 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     3961 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4021 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4081 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4141 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4201 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaatgat
     4261 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4321 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4381 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4441 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4501 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4561 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4621 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4681 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4741 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4801 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     4861 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     4921 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     4981 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5041 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5101 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5161 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5221 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5281 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc cgctttatat
     5341 gaagtacctg tcatcgagat agaaaaagta cgaataaaag aggatcatca attgattaca
     5401 ggaaaactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5461 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5521 ggagatgaat ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5581 atcttcattg gcgccatgtt gcttattggt ggaaaacagg gtctatttgc tttaggaagt
     5641 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5701 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5761 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     5821 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     5881 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     5941 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6001 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6061 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6121 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6181 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6241 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6301 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6361 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6421 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6481 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6541 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta
     6601 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6661 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag
     6721 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6781 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     6841 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     6901 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     6961 ccacattgtc cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7021 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7081 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7141 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7201 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7261 tcaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7321 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7381 gagaaagaca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7441 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7501 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7561 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7621 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7681 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7741 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7801 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     7861 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     7921 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     7981 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8041 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8101 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8161 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8221 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8281 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8341 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8401 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8461 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8521 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8581 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8641 gtgtttcaga tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt
     8701 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8761 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgatagtcta
     8821 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     8881 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     8941 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9001 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt
     9061 g
//
LOCUS       DAHEYX010000116         9029 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHEYX010000116 DAHEYX010000000
VERSION     DAHEYX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.117"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..132)
                     /locus_tag="MB219_001833"
     CDS             complement(<1..132)
                     /locus_tag="MB219_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCC8427548.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..2913
                     /locus_tag="MB219_001834"
     CDS             541..2913
                     /locus_tag="MB219_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427549.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2974..4434
                     /locus_tag="MB219_001835"
     CDS             2974..4434
                     /locus_tag="MB219_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCC8427550.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4445..6649
                     /gene="topB"
                     /locus_tag="MB219_001836"
     CDS             4445..6649
                     /gene="topB"
                     /locus_tag="MB219_001836"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCC8427551.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6665..6814
                     /locus_tag="MB219_001837"
     CDS             6665..6814
                     /locus_tag="MB219_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427552.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6895..7491
                     /locus_tag="MB219_001838"
     CDS             6895..7491
                     /locus_tag="MB219_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCC8427553.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            7504..8403
                     /locus_tag="MB219_001839"
     CDS             7504..8403
                     /locus_tag="MB219_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCC8427554.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            8406..8891
                     /gene="ssb"
                     /locus_tag="MB219_001840"
     CDS             8406..8891
                     /gene="ssb"
                     /locus_tag="MB219_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCC8427555.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         3397 a         1540 c         1913 g         2179 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaac
      541 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
      601 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaagaaag
      661 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      721 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaggta
      781 gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      901 gaatcgactt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     1021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     1081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgcacca
     1201 actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga gttgatcgat
     1321 caacttaaaa cgttggataa ccaatacgct gcagaatcag gttattataa attctatttt
     1381 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gttacgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaggac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcatgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaattgaa gtgatcaaaa aaccttacta cacgattgaa
     1981 aaagtgtttg atatcacaca aacacaattg aagcctgaag actatccgaa aatcttcccg
     2041 aaccgcgtct ttgattttca attggataaa acagggcaga cagaacttca agttgggatt
     2101 caagcggtag ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     2401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     2461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     2521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2581 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc tgttggttca
     2641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     2761 agttatggaa aagagattca aacggcgtta ccttcagaag aattttcgct aggaaatcca
     2821 ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga aatctattat
     2881 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact atctgaaaaa
     2941 cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat gagtcttatt
     3001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     3061 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     3121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     3181 tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc cacagatttt
     3241 attctaaaga atgaagacaa aacagaacga gtagaaaatc ttcattttga gacagaaaag
     3301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     3361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     3421 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     3481 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
     3541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
     3661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3721 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
     3901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3961 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
     4021 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
     4081 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
     4141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg ctcgaatggt
     4201 acgtacgatt ttgatcgttt ttatcaagga aaaggggata ttaatgctca gattcttttc
     4261 tcttataaag cagaacaata ctacaaaggc tataaaaagc atgaacatga atttgtacct
     4321 gaagtgaaaa aggattggaa cgatcaattg aagcatgaga tccagcaaca agaaattaga
     4381 aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt
     4441 cacgatgaaa actgtcattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     4501 cttttctaaa gccacccgaa aagacgggta ttttgagatc caagatcgat tgtttcctgg
     4561 tgaaactgtg attacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     4621 cgatgaaaat tggaaacaat ggtcattaga acatttacct attttcccta cacattatca
     4681 ctatcatgtt cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc
     4741 ggccgacacc attattattg cgacagatag cgatcgcgaa ggggaactga tcgcttggac
     4801 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     4861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     4921 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     4981 tagcctactg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     5041 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     5101 ccaaaaagaa ccatattttg aaggaaaatc ccaagtaatc gctcaaaatg gcgcatttga
     5161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     5221 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     5281 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     5341 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaaggaaa
     5401 atatctaagc tatccgcgaa cggatacccc ttatattacg gaaggggaat atgcgtatct
     5461 gttggatcac ttagacgaat acaagcattt tttaaaagcc gaagcgattc cgactcctat
     5521 tcatacaccg aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat
     5581 tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac tgcagcaggc
     5641 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     5701 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     5761 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     5821 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     5881 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     5941 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     6001 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     6061 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     6121 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     6181 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     6241 ctctcacttg ttggaagtcg taccaggaca aatccaatcc actgattttg gatcgacgtt
     6301 acaagaggtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag ggatttgtcc
     6361 aaaatgccag gaacaagaag ttttactcta tcataaggcg gccgcatgta cgtctgaagc
     6421 ttgtgatttt agattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     6481 ggaaatcatt caaaatggcc gaacaagcca gccagtgaaa ggattaaaag gtcaaaaggg
     6541 ttcgtttgaa gcaacgattg ttctaaaaga agacttcaca acgagctttg aattttctga
     6601 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat ggaggggtta
     6661 tcttatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     6721 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6781 acggattcaa aaagaaattg aattagatcg ataaaatctt cctggctatt gtatacgcat
     6841 aaacttatgt tatactatat ttataagttt atacgtatac aaaggaggga gaaaatgaca
     6901 aaagcaatgg tttacaagct gctagaagaa tttaacggaa ctttggcttt gaaggatgcc
     6961 aaaaaagcgg gcatttctcc tgtaacgatc aaacgtatgg tagatcgtgg agagctggat
     7021 cgtgagtatc caggtttttt caccttacct ggtcaatttc ctgatgaatt gtttatggca
     7081 caaaagaaat atgagcgcgg cattatttcg catatcaccg cgttggattt atatgattta
     7141 acggatatga tccctagaca gatcgatcta accgtacctt acggttatca cgtttctgaa
     7201 aaggggttga aagaattcgc agttgagtta cactattcaa aagcggaatg gtatgaatta
     7261 ggtaagatcg aaataaagag tcgttatggg aatcctgtta tagcttatga tccagaaaga
     7321 accttgtgtg acatttggaa tccctggtac aacgttgagg atgaaatcaa agtaaaagca
     7381 atcaaaaact acatggagtc ggacagaaaa aatcttagaa aattgaatga gtatcgacga
     7441 attttgccta cagacaaaac gatacgctct tatattatgg ctttaaatta aaggagaaga
     7501 cacatgacac ctacgcaatt aaaagcaaaa gtgaagaata tttccagaga aaaagaagtt
     7561 gatccgcagc ttgttttaag acattttatg atggaaaaat tcttggaaaa aatctcagag
     7621 tctccttatc gtgaaaattt tgtgttaaaa ggcggcttct taattggctc aaagtatggc
     7681 attgaaaatc gaacaacaaa agacattgat acgactttac gggaaatgaa agtaaccaaa
     7741 gaaacattga caacggttct aaacgatatt ttttcaacgc cgacgaaaga aggcattcaa
     7801 tttgagattc aaggaatgaa agaaacaagg gaagctgatt attatccagg gtttagctta
     7861 cgtgtactgg cacatttgga aaacatgcgg ccagatttta aggtagacgt gacgacaggt
     7921 gactcgatct atcctgcaac gatcactcac agtcataaat tgatgtttga aaatcgaacg
     7981 attcctttgg aatcttatcc tacagaacaa attattgccg aaaaattaag tgcaaccttt
     8041 gattttttaa cagataattc gagaggaaaa gatttttatg atctctatac tattcctaaa
     8101 atggaaaaga tcgatgagaa gaccttgaaa gatagtgtga gaaatacatt tacacgtcga
     8161 ggaaaaactg atccgttaaa gatttattac gaaaaggata tggatgtact gaaaaatagt
     8221 catgagctaa aagatacctg gaagaaatat caaaaaagca atccttatgc tgcgtcgatc
     8281 acgtacgaag agacaatgga ttcgattagt aatttgatgg aaaaagtgat tcacgtcgaa
     8341 gaaaaagagc gaaaaatagc ttatcaaaga caccaacaga tgaacagaga aatggagaga
     8401 taaaaatgat caataatgta actttagtag gacggttaac caaagatcca gatttacgtt
     8461 ataccgcaag tggcacagca gtggctactt ttaccctagc cgttaatcgt aatttcacga
     8521 atcaaaacgg caatcgagaa gctgatttta tcaattgcgt gatttggcgc aaaccagcag
     8581 aaacgatggc gacattagct aaaaaaggca tcttgatcgg cgttgtcgga cggattcaaa
     8641 cacggactta tgacaaccag caaggacaac gtgtgtatgt gacagaagtt gtagctgata
     8701 acttccaact attagaaagc aaagcagcga cagagagccg tgcgcacgct gatcaatcgt
     8761 ccacgagtcc aagcaccacc acttttgaac aacgggatac agcgacgcct aacaacaatg
     8821 gattgaacgc ttcacagaat ccatttggtg gacaatcaat cgatattagc gatgatgatc
     8881 taccgtttta agtagcattt tctattggtt gcatttgcta cggtttatgt tataatttag
     8941 tcaacaaaaa gaattttcaa tctataacca aaaaccacta gctggcgggc tagtggtttt
     9001 cttttacgtt tctattataa gaattaacg
//
LOCUS       DAHEYX010000117         8983 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHEYX010000117 DAHEYX010000000
VERSION     DAHEYX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.118"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            2..217
                     /gene="atpE"
                     /locus_tag="MB219_001841"
     CDS             2..217
                     /gene="atpE"
                     /locus_tag="MB219_001841"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCC8427556.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            308..832
                     /gene="atpF"
                     /locus_tag="MB219_001842"
     CDS             308..832
                     /gene="atpF"
                     /locus_tag="MB219_001842"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCC8427557.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            819..1361
                     /locus_tag="MB219_001843"
     CDS             819..1361
                     /locus_tag="MB219_001843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCC8427558.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            1384..2940
                     /locus_tag="MB219_001844"
     CDS             1384..2940
                     /locus_tag="MB219_001844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCC8427559.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            2954..3856
                     /locus_tag="MB219_001845"
     CDS             2954..3856
                     /locus_tag="MB219_001845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCC8427560.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            3879..5285
                     /gene="atpD"
                     /locus_tag="MB219_001846"
     CDS             3879..5285
                     /gene="atpD"
                     /locus_tag="MB219_001846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCC8427561.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     RGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            5299..5721
                     /locus_tag="MB219_001847"
     CDS             5299..5721
                     /locus_tag="MB219_001847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCC8427562.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            6190..6420
                     /locus_tag="MB219_001848"
     CDS             6190..6420
                     /locus_tag="MB219_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCC8427563.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            6517..7818
                     /gene="murA"
                     /locus_tag="MB219_001849"
     CDS             6517..7818
                     /gene="murA"
                     /locus_tag="MB219_001849"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCC8427564.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            7831..8007
                     /locus_tag="MB219_001850"
     CDS             7831..8007
                     /locus_tag="MB219_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCC8427565.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            8142..8438
                     /gene="yidD"
                     /locus_tag="MB219_001851"
     CDS             8142..8438
                     /gene="yidD"
                     /locus_tag="MB219_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCC8427566.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            8502..8885
                     /locus_tag="MB219_001852"
     CDS             8502..8885
                     /locus_tag="MB219_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427567.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
BASE COUNT         2953 a         1621 c         1949 g         2460 t
ORIGIN      
        1 tatgaattat atcgcagcag caatcgcaat catgggagca gcaatcggag ccggttatgg
       61 taacggtcaa gttatctcta aaacaatcga atcaatggct cgccaacctg aaatgtctgg
      121 tcaattccgt acaacaatgt ttatcggggt agctctagtt gaagctgttc ctatcttagg
      181 ggtagttatc gccttgattc tagtttttgc cgtttaattg aatcagtatt aaggcagaaa
      241 caacatcgtt tgttactgct tttttacatg gaaactagaa atactccaaa gaaaggaagg
      301 ctcgcctatg ctgaatcaat tggcaattgc agaagttggc aatgcgatgt taggtaatat
      361 catcgttgtc agcggctcat ttttgatact actggctctc ttgaagcact ttgcttgggg
      421 accaatcagc gatattttga aaaaacgtga agacaagatc gccaatgatt tagattctgc
      481 agaacaatct cgcatcaact cagcgaaaat ggaacaagaa cgcgaacaac aattgttagc
      541 ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa gaaagtggag aattaagccg
      601 ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt ctaaaaagca aagcccaagc
      661 tgatatcact gtagaacgtg attcagcgct gaactctgta aaagacgacg ttgcagaact
      721 ctctcttcaa atcgcggaaa aaatcttgaa caaagaatta tctccagaaa tgcatgaatc
      781 attaatcaat caatacattg aaggtctagg ttcttcaaat gaaactagat aaatatacag
      841 taggaagacg ttacgggaaa gctttatttg aactggcgat cgattcaaat caagcagaag
      901 aaatctatca agatctcgta agtttacgtc aaatctatga acaagtacct ggattaggga
      961 atatgttaag tgatgttcgt ctagaacctc atgaaaaacg agaaatcatg gacaagcttg
     1021 tcagcggtta tgatggaatc gttaaaaact ttttagaagt tgtttatgag tataaccgaa
     1081 tggatgacct tttgttcatg attgaagaat atgaacgtcg atatgatgaa catcaagggt
     1141 tactattggg aagtgtcaca acagcagtcc cactttcaga cgaacaacga atacaattag
     1201 aaaagaatgt tgcaaaaaca atggactatc aatcagttga attaaaacaa atcgttgatt
     1261 cttctattat tggtggcgcg attgttgaag ccaaccatcg agtaatcgac ggaagcattc
     1321 gcacgcaatt agaaaaaatg cgtaatcaat taaacagata gtacaaagag gtaggtgaat
     1381 cgaatggcca tcaaagcaga agaaattagt gccttgatta aagaacaaat tgaaaattat
     1441 caaaacgtgc tttcagtcga agaaatcggt accgtaactt atgtaggtga cgggatcgct
     1501 cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc ttgaattttc aaacggctca
     1561 tacggaatgg cgcaaaactt agagtcaaat gatgtaggta tcattatcct tggcgatttc
     1621 gagtccattc gtgaaggaga taaagtaaaa cgaacaggta agatcatgga agttccagtt
     1681 ggagaggcct tgatcggtcg ggtagtcaat ccgctaggtc aaccaatcga cggactaggt
     1741 gaaatcgtta ctgacaaagc tcgtccagta gaagcgatgg caccaggtgt tatgcaacgt
     1801 aaatctgtta acgaaccaat gcaaacaggg ctaaaagcga tcgacgccct tgtgccaatc
     1861 ggacgcggac aacgtgaatt agtcatcggt gaccgtaaaa cagggaaaac ttctattgcg
     1921 attgatacga tcatcaacca aaaaggtcaa gatatgatct gtatctatgt agcaatcgga
     1981 caaaaagatt ctacagttcg tacacaagtt gaaacattga aaaaatatgg cgcaatggat
     2041 tacacaatcg ttgtgaatgc cggtgcgtct caaccagcac cattacttta tatcgcacca
     2101 tatgctggta ctgcaatggg tgaagaattc atgtacaacg gtaaacatgt attgatcatt
     2161 tttgatgatc tttcaaaaca agctgttgct tatcgtgaac tttccttgct acttcgccgt
     2221 ccaccaggtc gtgaagctta tcctggggat gttttctact tgcactcacg tttattagaa
     2281 cgtgcagcaa aattaagtga tgatctaggc ggcggttcaa tgactgcttt gccatttgtt
     2341 gaaacacaag ctggagatat ctctgcttat atcccaacaa acgttatctc aatcactgac
     2401 ggacaaatct tcttagaaag cgacttgttc tacgcgggta ctcgtccagc ggttgatgcc
     2461 ggactatctg tctctcgtgt agggggttct gcgcaaatca aagcaatgaa aaaagttgct
     2521 gggactttac gtctagactt agcaagttat cgtgaattag aagcatttac acaattcggt
     2581 tctgatttgg atgcggcaac acaagcaaaa ctaaatcgtg gacgccgtac agtagaaatc
     2641 ttgaaacaaa aactgcatgc accgctagct gttgagaaac aagtagtgat tttgtatgcc
     2701 ttgacacatg gattcttgga cagtatccct gtagacagta ttttagactt cgaacatgaa
     2761 ttattcgaat atctggatac caatcattca gatatttttg aaacaatccg tacaacaaaa
     2821 gatcttccag aagaagaaac gttgaacagt gcgattcagg aatataaaga catgttctta
     2881 gctacaaaag gaaataattc ctctacagaa gataaattga aatccattca aaatgcatag
     2941 tgaggtgaga tgaatgggtg cttcattaaa tgaaatcaag acacgtatcg cttcaacgaa
     3001 aaaaacgagc caaatcacgc gggcaatgca gatggtgtct gcttctaagt tgacaaaatc
     3061 tgaagcttct tcacagaagt ttcaggttta tgcaacaaaa gtaagagaaa tcgttacgca
     3121 tttgactgct actcagttaa atgatattgc ctcagaaaat cctagagggg atatcaatta
     3181 caacagcatg ctgctcagcc gtcctgtgaa gaaaaccggt tatatcgtca tcactgcaga
     3241 tggcggtctg gtcggtggat ataacagttc gatattgaaa caaacgatgt ctattttaga
     3301 agaagaccac caatcacccg atgattacgt gatgatcgca attggcggaa ctggtgcaga
     3361 cttctttaaa gcacgcggta tcaacctagc atatgagctg cgcaatttat ctgatcagcc
     3421 aagctttgat gaagtccgca aaatcgtaaa tatggcaaca actatgtacc aaaacgaagt
     3481 attcgatgaa ttatacgtat gttataacca tcatatcaat tcgctaacta gtcagtttcg
     3541 tgtagaaaaa atgctgccaa tctcagactt agatccagaa gaagcaacga ctttcgaaca
     3601 agaatatatt tttgaacctt caaaagaaga aatcttgtct cagttattgc ctcagtatgc
     3661 tgaaagtttg atctacggtg cgatcgttga cgccaaaaca gcagaacatg ctgctggtat
     3721 gacagcaatg aaaacagcaa ctgataatgc tgctaatatc attgatgagc tgacggtttc
     3781 ttataaccgt gcaagacaag gggctattac ccaagaaatt accgaaattg ttgccggagc
     3841 ttcggcacta gaatagtatt gagattggag gaaaaaagat gagttcaggc aagattgttc
     3901 aagtaatcgg tcccgttgtc gacgtggaat tttctttaga ccaatcctta ccagatatca
     3961 acaacgcatt agttgtttat aaaaatgacg aaaataaatc aaaagttgtt cttgaagcag
     4021 cgttagaatt aggggacgga gtgatccgga ccattgcgat ggaatcaaca gatggtttac
     4081 aaagaggaat ggaagtcatt gatacaggca aagcaatctc tgttcctgta ggtaaagaaa
     4141 cattaggtcg cgtattcaac gtactaggag atacgatcga tttagaaaca cctttcccag
     4201 aagatgcgga aagaagcgaa attcataaaa aagcaccagc ctttgacgaa ttaagtacaa
     4261 gtacagaaat tttggaaaca gggatcaaag ttatcgattt gcttgcccca tatttaaaag
     4321 gtgggaaagt cggactattc ggtggtgccg gtgttggtaa aaccgtactg atccaagaac
     4381 tgatccataa tatcgcccaa gaacatggtg gtatttctgt atttaccggt gtaggtgaac
     4441 gtactcgtga aggtaatgac ttgtattatg aaatgaaaga ttcaggagtt atcgaaaaaa
     4501 cagccatggt gttcggacaa atgaacgaac caccaggtgc acgtatgcgt gttgctttga
     4561 ctggtttgac gattgcggaa tatttccgtg atgtagaagg tcaagatgta ctgttgttta
     4621 tcgacaacat tttccgtttc actcaagctg gatctgaagt atcagccttg ttaggacgga
     4681 tgccttctgc ggttggttat caaccaacat tggcaacaga aatgggtcaa ttgcaagaac
     4741 gtatcacatc tacgaaaaaa ggttcgatca catcgatcca agcaatctat gtgccagccg
     4801 atgactacac tgacccggcg ccggcaacag cattcgccca tttggatgcg acaaccaact
     4861 tggaacgtaa attaacagaa cgagggattt atccagcggt agatccattg gcttcttctt
     4921 caagtgcttt agcacctgaa atcgtgggag aagaacatta taaagtagca acagaagtcc
     4981 aacacgtatt gcaacgatat cgtgaattac aagatatcat tgctatctta ggtatggatg
     5041 aattatccga tcaagaaaaa gtgttagttt ctcgtgcccg cagagtccaa ttcttcttgt
     5101 cacaaaactt taacgtggct gaacaattta ctggtcttcc aggttcatat gtccctgtag
     5161 aagaaacagt gaaaggattc cgcgaaatct tggaaggcaa atacgatgat cttcctgaag
     5221 aagctttccg aagtgttggc cgaatcgaag acgtcgtaga aaaagcgaaa acactaggct
     5281 actagaaagg ggtgccccat ggatcaatat ttaacagtaa atgtggtaac tcctgccggt
     5341 ttggtctatg atcaccatgc agctatcgtc gttgcacgta cgacagctgg tgagcttggg
     5401 attttgccta aacatgcacc aatcatcgtt ccattgacga ttgatgaagt tcgtgtaaaa
     5461 cgaacagatt cagataccca cgtcgattgg atcgctgtta atggcggaat catggaagtt
     5521 cgtgataatg ttgtttcaat cgttgctgat agtgcggaac gtgaacgtga tatcgatgta
     5581 tctcgtgcgg aacgtgcaaa acaacgcgca gaacgccaaa tcgaagaagc aaaagcaaaa
     5641 caagatacga acgaattgaa acgggcaact gttgcgttgc accgagcaat caatcgtatc
     5701 aaagtatcta agcactcata aaatgagatg cggatgttta tccgtgttta agaacagtaa
     5761 aaagaggtta tgacagaagt ggtcagcttc aagacataag aaggaattta cgaaaattgc
     5821 ttctcaaatt tttgtgaaat ctcagcttat ttttcgaaga agctacttct ggaacaccgt
     5881 atatcagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa atggaaaata
     5941 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagatgatt gaacaccgtt
     6001 tatttggaat aagggactgc gacaagactt ttgtctcagt cccttttttt gcatattttt
     6061 agcatttatt tcctaaaaaa tgtaagggga ttcctatatt tttaagtata cttgtttttc
     6121 cttttataca ttttatggta ttatcatttt attagttatt ataaaacaat cgttagaaag
     6181 gacgtcatta tgcaagtata cggaattgat gcggtgcttc gaatcgtgag tcatttggcg
     6241 tttatctact tagccttctg gtctttacgt tcgttacgca ttgaaacttt tttcagacca
     6301 atgcataata cgcaaattag aatggcaatc gtactttttt caattttttt agggtacact
     6361 gcaagtaatt ttttcttaga agttatcgct ttatgccgaa atcttttcgt tagcctgtaa
     6421 attttaaaaa aaggtactat tttaaaagca aggatgttat aatagaaaag attttgagta
     6481 agtgtatgct cattggaatt ttttggaggg aacaacatgg aagagatcat cgtaagaggt
     6541 ggcaatcaat taaatggaac agtaagaatt gaaggagcta aaaatgctgt tttgcctatt
     6601 ttagctgcta gtttgttagc agaagaaggt attacgacat tagacaacgt tccgattctt
     6661 tctgatgtct tcactatgaa tcaagtgatt cgtcacttaa acgtagatgt agattttgat
     6721 gaacaaaaga atcaagtaac aatcgacgct tcaagacaat tagaaatcga agcgccttat
     6781 gagtatgtta gtcaaatgcg tgcttcaatc gttgtgatgg gtccgctact tgccagaaat
     6841 ggacatgcaa aagtagctat gcctggtgga tgtgcgattg gtaaacgacc aatcgatttg
     6901 catttaaaag gatttcaagc tttaggagca aaaattattc aaaaaaacgg ctatatcgaa
     6961 gcaattgcgg atgaattgat cggtaacacg atttatttag atttccctag tgtgggtgct
     7021 actcaaaata tcatgatggc tgccgtaaaa gcaaaaggaa caaccatcat tgaaaacgta
     7081 gctcgcgaac cagaaatcgt tgatttagca aacattttga ataaaatggg cgctcaagtc
     7141 tatggagctg gtactgaaac aatgcgtatt gaaggcgtgg atcatttaca tgcagtcaac
     7201 cactcaatcg tacaagatcg tatcgaagca ggtaccttta tggttgctgc tgcaatgaca
     7261 caaggcaatg tgctgattgc agatgctatc tctgaacata actgcccatt gatttctaaa
     7321 ttgatcgaaa tgggtgcaga aatcattgaa gaagaaggcg gagtccgtgt catcggacca
     7381 aaacatatct tacctacaga tgtaaaaacg atgcctcatc ctggtttccc tacagatatg
     7441 caagctcaaa tgacagctat ccaattagtt gcagaaggta caagtgtggt gactgaaaca
     7501 gtgtttgaaa atcgtttcca acatttagaa gagatgcgcc gtatgaacgc tcatgtcaaa
     7561 atcgacggaa atgtagcaat catggatgga aaccatgaat tgcaaggtgc agaagtatat
     7621 gcaacagact tgcgggcagc agcagcatta gttttagctg gcttgaaagc aaatggaatc
     7681 acacgtgtgc gtaacttgaa ttatctagac cgtggatatt ataatttcca tatcaaacta
     7741 cagcaattag gtgcagacgt cgaacgtgta gacatggatc aaacttctgc tgaaaaaaca
     7801 gcacaaacga ttgcttaagg aagtggctga atgagtaacc aacgatatat cttattaact
     7861 ttgataaaga ttttagtggt gatcttattg cttatccttc tttttgtggc cggaacgatg
     7921 atcggatatg gtgtcatcgg tggcggcaat ccatttaaag tattccagcc aagtttgtgg
     7981 attcatattc gagacttttt tcattgataa ataagaatca cagtggttct agtcgatatg
     8041 aagtgagcca tgaagcagaa catgataaaa gaaaacagaa gggaacgttg acgttagtcg
     8101 cgatcccttt ttttagtaaa agaaaagaag gaatctaatt gatgaagaag atattgattt
     8161 atttagttag aggatatcag cgctttattt cgcctgcatt tccacctagc tgccgttatt
     8221 acccaacctg ttccaattat atgataaaag caattcaaac ccatggtgcg ctaaaaggga
     8281 cggtaatggg gactgcacgg atcttacgct gtcatccttt tgtcaaaggc ggtatcgatt
     8341 atgtaccatt gacattccga ttaacgcgaa ataagaaaga taaaaaagaa ccagattata
     8401 aaaaggttgt gaaaaatacg ggtagcactg agcgataaag aaaaagagga aatttattgt
     8461 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     8521 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     8581 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     8641 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     8701 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacagc
     8761 tagcttcaaa ccaaactcgg atcattatac gtactggcac tactagtagc gctgcgttac
     8821 acctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     8881 attaatctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     8941 ttcacttacc taaaaatagc tttaactaat aaactttcaa ttc
//
LOCUS       DAHEYX010000118         8855 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHEYX010000118 DAHEYX010000000
VERSION     DAHEYX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.119"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            101..1162
                     /locus_tag="MB219_001853"
     CDS             101..1162
                     /locus_tag="MB219_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCC8427568.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1165..2061
                     /gene="murQ"
                     /locus_tag="MB219_001854"
     CDS             1165..2061
                     /gene="murQ"
                     /locus_tag="MB219_001854"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCC8427569.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2075..4057
                     /locus_tag="MB219_001855"
     CDS             2075..4057
                     /locus_tag="MB219_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8427570.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4122..4568)
                     /gene="nrdI"
                     /locus_tag="MB219_001856"
     CDS             complement(4122..4568)
                     /gene="nrdI"
                     /locus_tag="MB219_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCC8427571.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4779..6203
                     /locus_tag="MB219_001857"
     CDS             4779..6203
                     /locus_tag="MB219_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8427572.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6384..7157)
                     /locus_tag="MB219_001858"
     CDS             complement(6384..7157)
                     /locus_tag="MB219_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCC8427573.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7154..8059)
                     /locus_tag="MB219_001859"
     CDS             complement(7154..8059)
                     /locus_tag="MB219_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8427574.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8213..8533)
                     /locus_tag="MB219_001860"
     CDS             complement(8213..8533)
                     /locus_tag="MB219_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCC8427575.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2790 a         1583 c         1879 g         2603 t
ORIGIN      
        1 atgaaattta attattttaa taaaagtatt gaaattttgt ttctttagat atatactcta
       61 gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct tttcaatatt
      121 tatgaacgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg catccaatgg
      181 ctttgtcggt attttcactt ccatgcatat accagaagat gatgtttcgg catatcaaaa
      241 gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagaattga tggtcgatat
      301 ttcaggagag gcattggaac gagcgggttt ttcattagaa gatctaacgc ctttgaaagc
      361 aattggtgta acaggtttaa gaatggatta tcatatttcc aatcagcaga ttgccgactg
      421 gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag atatcgatga
      481 attgaaagaa gcagaagctg atttctcaca gattgaagct tggcataatt attatccacg
      541 tcccgaaaca gggctggaca aagaatggta tcagaaaaaa aatcaatggc tcaaaattca
      601 aggtctcctt gtccaaggtt ttgtaccagg agatacagag ttgagaggcc cactatatca
      661 aggattgcca actttggaag aacatcgagg gatccatcct cttgcaggcg ctctagatct
      721 attagcaagt aacacggata ttgtttacat cggagatgca ggtctgtcag aaaacgttca
      781 ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg aacctgtaga
      841 tgaagaattt tacgaatata tattagggaa gcatacgaat cggcaagacg atgcaagaga
      901 tgtcatacga agcgtagatg cacgttttcg agagattccg cctatcccag caagaaatac
      961 agctacgcgt atgaaagggt ctattacttt agataacgaa aagtatttgc gctatatggg
     1021 tgagatccaa ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg tagtagctaa
     1081 agttatcaaa gaagagctac cattgatcaa ccaaatcaaa gctggtatga actatcaatt
     1141 tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct aacaacggaa
     1201 cgcagaaacc aagaaacttt tggattagat gagatgagta tttctcaagc cgttcaacta
     1261 atgaacaaag aagatcagaa agtggccgct gcggttgaaa aagaactgac aagtatcgaa
     1321 ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat ttatatggga
     1381 gccggaacaa gtggaagact aggtgtgtta gatgctgctg aatgcgtacc gacattcggt
     1441 gtagaaccgg aaatggtcat aggtctaatt gctggtggag aagaagcaat gactgtagct
     1501 gtcgaagggg ccgaagattc ttatgagcta gggcaagaag atctaatcaa gctgaatttg
     1561 acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata tgtgatcggt
     1621 ggattagatt acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg caataaacaa
     1681 gccgaaatca gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc agaatttttg
     1741 acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa tatgatttct
     1801 actatcagta tgattggtat aggtaaagta ttcaataact tgatggtcga tgtcaaacca
     1861 acaaatgaaa aactaatcga acgctcaaaa cgaatcatca tgcaagcgac agaagctgat
     1921 tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct ggctatcgtc
     1981 atgattttga caaatagtga taaagaggaa gctcaagcaa aattagctga agcaaacgga
     2041 tttgtaaaag ggaccttatg agggggaaaa gaccatggca aaaaacgaac tgactttgga
     2101 agaactggca aaacaaattt ataaaggggc aggcggcatg ggcaatgtag aaagcattgt
     2161 ccactgcatg actcgtgtgc gtatgaagat catcaatgat gatcttgtaa atgtctctga
     2221 attaaaagcg attccaggag tgcttggtgt agtagaagat gaacagcttc aagtcatcat
     2281 cggacctggg aaagtgaaca aagtagcaca gcaaatggtt gatttggcag gcgtgaaatt
     2341 aggagaaaaa ctttcttctg taagtacagc tacagcaaat tcatcagcat ctggaaaaga
     2401 ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac aaaaaccatc
     2461 taaaatcaaa tcggtattaa aagacatttc taatatcttt gtgccaatga ttcctgcatt
     2521 tgtcggtact gggatcgtgg cagggattgc cgcagtttta gcaaacttag tcacagccgg
     2581 aacacttgat gcagcaacat ggcaacaata tatcgatgtg atgaacatct tgaaaaacgg
     2641 gatgtttagc tacctagtta tctatacagg tatcaatgct gcacaagttt ttggtgcaac
     2701 accaactctt ggtggagtta tcggggccgt tgtgatgctg acagggatga atccggaagc
     2761 accactgaaa aacttattta ctggtgaagc tcttgcagct ggacaaggtg ggatcctagg
     2821 tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa aaactgcata aagtagtacc
     2881 tgatgcggta gatatcatcg ttacaccaac tctttcattg attgccatcg gtctgattga
     2941 aattttcttc atcatgcctt tagcaggttt catttctgat ggcatggtcg gtgggatcaa
     3001 ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat tattcttgcc
     3061 gatggttatg ttcggactgc atcaaatttt gactccaatc catgtgcaaa tgattgatga
     3121 aactggtcgt acgctattgc ttccaattct tgcgatggct ggtgcaggac aagtgggtgc
     3181 agcattggca ttatggattc gctgcaaaaa agataaagaa ttaacagaaa tgatcaaagg
     3241 agctttacca gtcgggatcc taggaatcgg cgaaccattg atttacggag taacattacc
     3301 tctaggacgc ccatttatta ctgcttgtat cggtggaggt atcggcgggg cagtgatcgg
     3361 aatgatcgga aatgtcgggg ctatcgctat tggaccttca ggagccgctt tgatcccatt
     3421 gatctctgac ggtaaatggt taggctacat ccttggatta ttggcagctt atgctggtgg
     3481 cttcgttgct acattcttct tcggtattcc taaagaccgt ttagaaaaag aagaattagc
     3541 gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta gtacctcaaa cttcagctga
     3601 aagtgtgact atcctctcat cagttgctga cggaacagtt gtttctttag ataaagcaag
     3661 tgatccagtt tttgcacaaa agatgatggg tgaaggctac tttgtagaac cagaaaacgg
     3721 acatatctat tctccagtta ctggggtagt cagctctgtc ttcccaacga aacacgctat
     3781 tgggatcaca acgccaaatg gtttagaaat cttgcttcat atgggaatca acacagttga
     3841 cttaggcggt acgccatttg atgtgaaagt aacagaaggg cagcaagtga cacctgatac
     3901 gcttgtagca gaagctgact tagaagcgat caaagcagca ggcaaagaaa catccatgat
     3961 cgtcttggta accaatatgg atcgtgtaaa aaactttgtc ttggaaaaaa caggtaaagc
     4021 aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat gatagaaaca atagaaacga
     4081 aagaagaaac tgaaatctat ttttcagctt cttctttttt attatttatt gttttctgct
     4141 aaaatctgat aaattcgtgt cacatcagat ggtgttcccc gtaattcata atctgccaag
     4201 aaaggaaagc caaatgtttg tgcatattgt ttggctgtta gacaatactg attattgaag
     4261 tttttatttc cgctaccgac tacaccaaga caaagtttat gattgtcttc atgttccaga
     4321 tattcacgca tcgtttcggt caagatttct gtatcaccgt tatctaaacc atttccgccg
     4381 tctaaataag taggaacgaa agtaaaaaat ggttctgttt ctttcgtaaa atcactattt
     4441 tcatggattt cttttaaagt aacttcagga ttgacaggat cttctgcatg cattttttca
     4501 gcatattctg ctagatgttt ggcaaaggca cgcgtatttc ctgaaatcga tatatataaa
     4561 atgttcatgg atcatcctcc aattctttcc ttttagtata gcatcttttt taagtttttt
     4621 ggtggtgtaa tttggaaagg gactaggcgc tttgcttgta taatgagaaa aaagccggta
     4681 gaattatatt tctattaaag tttttattaa ttaagtaaaa ataaaaaagg tttgttatac
     4741 tttttgataa aagcggtttt attttggagg atgcttacat ggatacagag caacagaaaa
     4801 aaggaattgg tttaattcca cttgctgcct tagtaattgg ttcagcgatt ggaggtggcg
     4861 tatttggtat tatgacggac atctccggat cggctggcgg cccagctatt ttcagttggg
     4921 tactcgtagg aacagcaatg ttgatgctct ccttgtcgat caataatatc aataaaaaac
     4981 gtccagattt aaaaggcggg atctttagtt acgcagaaga aggttttggc cgtttcgctg
     5041 gttttatcag cggttggggt tactggctca catgctggtt aggcaacgta gcttttgcaa
     5101 cattgttgat gagcgcatta ggatatttct tcccagtatt tgaaggtggg caaaatctgg
     5161 catcgatcgt gatgagtaca gtctttttat ggggatatgc atggctagtg aatcggggag
     5221 tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg taaattagtg ccattatttt
     5281 tatttattgt agtagctatc agcacgtttt cgatccaaca attcattgat aacttttcta
     5341 gcgacattat attgaatgcg aatggacagc cgatcccgtt ttccagtcaa gtattgagtt
     5401 gcatcatggt gatgctttgg gtatttgttg gtattgaagg agcctctgtc gtaggttcac
     5461 gagcaaaaaa acaatcagat gttgggaaag caacgattct agggatattc ggcttgatta
     5521 ttatttacgt cttggtttcc atgcttccat atgggacaat gacgatgaat cagctgcaaa
     5581 cgatcaaggc gcagcctgct atgggctatg tgtttgaaat gatggttggt aaatggggag
     5641 cagtcgtgat caacggcggt ttgatcattt ctttagttgg tgtttggctc tcatggacga
     5701 tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg acttttgcct aaagcatggg
     5761 gaaaactcaa caaaaatggg gcaccaactt atgctatcgt attgacgacg ttatgtacga
     5821 atgtcttttt actttcatta ttattcactg acagtgctta taactttgct tacacacttg
     5881 gtacagcagc tattttcttc acgtggcttt tcttaggatt gtaccaaatg aaactttctt
     5941 atcagcggaa agaatgggtc caatttttag tagggctttt agcaagtgcc tttcaagtat
     6001 gggctatgct agtcgtagcc tttaatgaag taatgcttgt tcttgttctt ttcttgccag
     6061 gcatttattt ttacctcaaa gcacgtaagg agcaaaacga gcctgtacaa catatccact
     6121 tagataagat tttacttata ggtgtgacga tggtcggagt gattgcaatg gttttatttg
     6181 ccactgggat gcttcacgta tagaagtata aaagaagtat aaagagaggt tgtgagcctg
     6241 tcgtttattc aaaaacatac taggaaagga gctgtgacac aaatgttgtc acagctcctt
     6301 tttcagaata aacggtgttt aagagacagc caatcggcaa taaggctgaa cgtctctttc
     6361 acagcctctt ttttgatgag tcatcataca ttttttcgct taaatacgag atatcctaat
     6421 gctaagaaca aaacgatgta agctaaatta ccgaatagca tttgattagt agaaagatga
     6481 gtcaattgct tcatgatctc ttcgtttcca atttggtttt gcagattcaa catactgatc
     6541 ggattccatt tgatccactc ccatttttgg atgagtgcaa taaggatacc agatatgatt
     6601 gacgaagcaa aataaaagac gattcctgct gagatggatg caccggaact attgataaag
     6661 caagcaatca tcaacactaa acttaaaatc agccataaac cgatataact acccaaagta
     6721 taagtgaaca agctttggat cagtgtttga ccagtagaaa ccttctccgt gaaggaaatc
     6781 gaagggaaaa gaatcagttt catcaacaca gtgacaataa tagctaaaag gtataaataa
     6841 acagaataaa taactaatgt gatccatttg cttactaata tttctcctcg agaatacttt
     6901 cgataaagga gatttttcaa tgtaccatat tgtgcttcca tcgaaatgat cgtgcttgca
     6961 gctgcaatca tgataaagac aatccaagat gtggcactaa aaagctgagc agtcatttca
     7021 actggatcaa tgatcgtcgt gtattttttg gctagaagtg ctgttcctat gagcaaaaca
     7081 actaatagaa cagataaaac agcagttgac tttttcttga tcaatttaaa taattcttgt
     7141 tttacaagca atgtcatttt tcttcttccc cctttactga atccagaatt tccaatagag
     7201 aagattccaa atcattcgtc agatgctgga cgtctgtcac atggatattt tgtgcagaca
     7261 atagctggat cacggttcct aataattctt cgttttcttt taaaagcttg attttatcag
     7321 gatcttccaa agtatatcca gcttctgtca agatcgttcg agcagctaga tcatttgttg
     7381 tagagataac gatgaaatct tgcgctgttt ggttcaattc agccattgta cattgtttga
     7441 tgatctttcc ttgattgatc aggatcagct catcgaccat tttttcaagc tcgctgagta
     7501 agtgactgga gatcaagaaa gaggtgccct gagtagccaa gtcggtaatg atttttcgca
     7561 catctcttgt tgcttgtgga tccaaaccgt tcatcggctc atctaaaata acgatagacg
     7621 gatggttcag taaggcaaga gcaattccca atttttgttt cattcccaac gagtaggatt
     7681 tcgctttttt gtgaatgtac ttttccatct tgagctgatt cacgatagtg tcgatagcag
     7741 atacatcgtt ggtttcagag aataacttca gatgatcata gcctgttaag aatggataga
     7801 tacccgggta ttcgatcaat gctcctactt gttccaaccc tttatgacta gagggagaaa
     7861 tctcattttc accaatacag attgttccat ctgagtaatg gatcagtcct aaaatagctt
     7921 tcatgatcgt tgtttttcca gcgccattag gacctactaa tccaacgatt ttgccagggg
     7981 ataagctaaa actgacatcg ctgagggctt gtttgtgacc aaagttcttg gataattctt
     8041 ggaccgaaag acttttcaaa aatattcact ccttttctat agatagtaca agcatacacc
     8101 tgatgagtgg agaagtcgct aggtcattgg acttatttct tattttttca taaaaaaatt
     8161 gaatattaaa taaaaaggct tattttttct gatcagtact tattccaagt gtctagtggc
     8221 cgctagtaat cttgatgcca atgatactga tgagtagcat ccccacaaag aaccaagtgg
     8281 aaagattcag cttatcatga aacaagacca caccaattat gaccgaacca actgcgccaa
     8341 tccctgtcca gataggataa gagagactca tcggcaaact tttgattgcc tttgacaaaa
     8401 agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga ataattgatt ttagaaaaac
     8461 catcactcca tttcatcatc gttgaccaga atacttccaa aattcctgct atgattaatt
     8521 ccatccatgc catacttaca ttcctcctta actataaaaa aatacacaaa aagaggaaaa
     8581 tcatcccttc aaagacctaa tggaatgatc atcctcttta tcctacccgg tggcagattg
     8641 tatatctttt tctgtagtat agcacaccta tccagatatc ggcaatctca tactttttag
     8701 taaagtgtac caagcactga cggatatatc gttagcggaa aagaagatga atgctttgca
     8761 atttcataga aagaagttgt tatttattaa tcatttaaaa aaaacagtaa ctagacctta
     8821 tttctatata ttatcgggca ataaattaaa aaaag
//
LOCUS       DAHEYX010000119         8830 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHEYX010000119 DAHEYX010000000
VERSION     DAHEYX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.120"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(15..1379)
                     /locus_tag="MB219_001861"
     CDS             complement(15..1379)
                     /locus_tag="MB219_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCC8427576.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1376..2935)
                     /locus_tag="MB219_001862"
     CDS             complement(1376..2935)
                     /locus_tag="MB219_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427577.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2991..5471)
                     /locus_tag="MB219_001863"
     CDS             complement(2991..5471)
                     /locus_tag="MB219_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427578.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5455..5790)
                     /locus_tag="MB219_001864"
     CDS             complement(5455..5790)
                     /locus_tag="MB219_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427579.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5804..6838)
                     /locus_tag="MB219_001865"
     CDS             complement(5804..6838)
                     /locus_tag="MB219_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCC8427580.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6928..7608)
                     /locus_tag="MB219_001866"
     CDS             complement(6928..7608)
                     /locus_tag="MB219_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCC8427581.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7778..8737)
                     /locus_tag="MB219_001867"
     CDS             complement(7778..8737)
                     /locus_tag="MB219_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCC8427582.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT         2508 a         1753 c         1611 g         2958 t
ORIGIN      
        1 gaccgtgata tttctcacag tcttttatct actctattgc tatttgctct cttagctagt
       61 actttaaaag ctaactcatt gaggtagtga aaacgagagg tgtattcaga cagaaatcca
      121 aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg
      181 ttcaaatctg ataataagaa ttcttggctt atcagctgtc cgccaattat ttctaatgta
      241 tccggatggt agatcagctt tgcatttact gttttgtttt cttcgtcgta ttgtggaaat
      301 agatcaaatt ctcctgaaca cgaaccggta ttttgttcca agaatggcgc ttcatcttct
      361 gtcagcccta cagttcctaa aaagtagtct ataatgactg ctgctgttat cttttgtctg
      421 aggtcgtaag cggttggctg accactcaaa gttagcgcta ctgctcttgc catatgtaca
      481 gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt tgtcaaaact gactgggacg
      541 atatctccaa tcgcatagat gtccggatca cttgtctgca gatattcatt gactatcaca
      601 gtctcatcct catacaaagc cactttttct tttaacaatg tcgtattagg acgagtttgg
      661 gcagaaaaaa tgaccatatc tgcctttatt gtttgagaca gaagttgtac ggatactact
      721 ttctcttctg attttttcca atcgatcaag aattcatttg gataaaaatt tatttgctta
      781 gtttgcagct gattttgaaa actgttgatc atatccttat cgaaatatct tgataaaaca
      841 ttcggcatat aatcaactag atgaataatg tatcctttat ttctcaaagc atctgctaat
      901 tccaatccaa tgtaaccagc accgaagatc actacttctt ttgcttcttt taaagcggca
      961 agtacttgct tagattgcac tagattttta taggtgtaga cattttcagc ttcaatttgt
     1021 ttatacaagg tgacaggaga ggaacctgtc gctacgatta atttatcata ggttatggtc
     1081 tcgttttttc cttctttgga tgcgaggatg agctgatgtt tgtctggatc aagttcagtt
     1141 actctccatt cggtgatcag ttttgcccca ctttcttgaa gagtccgcat gttatgggca
     1201 acttcagcta aactacgtat ctttcctttt aaataaaggt tgattccatt tgaaacatag
     1261 cctagtccat ctttatgatc ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt
     1321 cttagattta gggcagctgt gatgccttca tgggaagcac caatgatcac gattttcatg
     1381 ttttattcat ccttaaattc aatttttcta aataacattc ttttaaaaat tggttcaaat
     1441 aggggaaatc ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
     1501 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
     1561 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
     1621 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
     1681 tgatcagcgt ccgtaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
     1741 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
     1801 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
     1861 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
     1921 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
     1981 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
     2041 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
     2101 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
     2161 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
     2221 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
     2281 atttcaatcg tccttcttct gtaatagcaa aactaaactc tttttgcagc agatctaaaa
     2341 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataggga cgtccaaacc
     2401 aaaataattg gaaataaaaa taacgaattt gactttctgg accggtcagc cgcccgcttt
     2461 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
     2521 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
     2581 acagataaag aagtatctga tattttgtag aatagcgcaa gtaacaatga taataaacat
     2641 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
     2701 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
     2761 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aacctctcct ttttcctcta
     2821 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
     2881 ccaagtccac tccttcaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
     2941 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
     3001 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
     3061 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
     3121 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgc
     3181 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
     3241 ttaaataaag aagtcaatgt acctgtcagt tgccaaggag acgtgcttgg aagccgagta
     3301 tcccgcacat caaaagaaaa atcgcctttt gattcataat aacttgtctt caacggtttg
     3361 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
     3421 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
     3481 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaaa
     3541 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
     3601 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
     3661 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
     3721 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
     3781 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
     3841 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
     3901 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
     3961 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
     4021 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
     4081 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
     4141 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
     4201 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
     4261 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
     4321 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
     4381 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
     4441 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
     4501 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
     4561 gttgaatcac ttggtgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
     4621 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
     4681 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
     4741 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
     4801 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
     4861 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
     4921 tctgtcaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
     4981 gctgataata aacttccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
     5041 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
     5101 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
     5161 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
     5221 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
     5281 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
     5341 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
     5401 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
     5461 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
     5521 ccataattct ataccaatga tcaaataaag aaaacggatc ttttgttcat tcgtctccgg
     5581 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct gttttgatgc
     5641 gccattgact gaaccacttg gatgcggaat tggacttaga tcataaaatg taatcgtacc
     5701 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
     5761 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
     5821 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
     5881 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
     5941 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
     6001 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
     6061 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat gcattagacg
     6121 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
     6181 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
     6241 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
     6301 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
     6361 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
     6421 ttgctgggat tttcacttct ataggtacag ttgtttctgc atgtgctgat acattgattt
     6481 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
     6541 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
     6601 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
     6661 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
     6721 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
     6781 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
     6841 tccaccacat cctttcaaca acaactgcgg taaaaaatcc ttcacctata gtattttcac
     6901 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
     6961 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
     7021 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
     7081 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
     7141 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
     7201 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
     7261 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
     7321 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcgggaac
     7381 tacatccagt gttaaaggac ctgtctggcc agttgctgga ttttccggat ctgctggctc
     7441 tactggtttt gaaggatcag tagggttaag aggctccacg gtatttgttg gttcagtaaa
     7501 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
     7561 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
     7621 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
     7681 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
     7741 acatgaatat tcttcctttt ttattaagtt tcgtttgtca aattaagtta gacaaatcat
     7801 cttttgtgac gtaactcaaa aatacttcca ttggtgtccg atagtgtaat gattttctag
     7861 gaatatgatt tcgtttcgat gccaccgatt ggataaaggt ttcagataca ccattaaaat
     7921 ccatagcttt atacaaacca tctttacgta ataagccatt tgaatgttca ttcaatcctc
     7981 gttgagaagg tgttcctgga tccgcaaaat aaattgaaat atcgttggta ttactaatct
     8041 ttttccaatt tgagaattct tttccacaat caaatgtgat ggatttaaat aagttttttg
     8101 ggacacaacg aaaccattga ttcagcctat tttcaatgtc tttagcctgt ctgccttcgg
     8161 gtttcaaggt aatgatgact ttagatagcc gttcaactaa tgtgatgacc gcgcttttat
     8221 gttttccacc cacgatagtg tcaccttcca ggtgaccaaa ttcatgatca aactgggtat
     8281 agtctgtctt tctgtctcgg attgaccggc gaaacccttg ttttcctctt ttttctttat
     8341 gaccgtttgg ttttcttttt cccttcattg gtaaatcgtg agtgtcaaag gttttatctt
     8401 taaacattcg atacagtgtg cgtaccgaac aagcaatagg aaactctgct cgaccaataa
     8461 ggacatcagg agcccaccct tgaacaacct ttttttgaat gtattcctgt tgctcatcag
     8521 gtaaaacaat cggatgtctt ccgcagttac gtttgttttt cttatattgt ttatagtagc
     8581 tcattgcgct tccaccagat ttaagaaaac gatagacctt ataaatagtt tgtcgagaac
     8641 gttgtaacag attggctact tttgaaacag gttgtgattg attgaaataa gcttctatca
     8701 ttacgagttc agttggtgta agatggttat aggtcatttg tactcactcc ttataattct
     8761 ttcgtcggaa ctattttgag tgtagcacaa atgacttttt ttagttgtct agcttaattt
     8821 tacaatcggc
//
LOCUS       DAHEYX010000120         8670 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHEYX010000120 DAHEYX010000000
VERSION     DAHEYX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.121"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(8..2389)
                     /locus_tag="MB219_001868"
     CDS             complement(8..2389)
                     /locus_tag="MB219_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCC8427583.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MB219_001869"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="MB219_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCC8427584.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="MB219_001870"
     CDS             3090..3629
                     /locus_tag="MB219_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCC8427585.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWDEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="MB219_001871"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="MB219_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCC8427586.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="MB219_001872"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MB219_001872"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="MB219_001873"
     CDS             4736..5902
                     /locus_tag="MB219_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCC8427587.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="MB219_001874"
     CDS             5948..7444
                     /locus_tag="MB219_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCC8427588.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMEDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="MB219_001875"
     CDS             7525..8208
                     /locus_tag="MB219_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCC8427589.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2724 a         1570 c         1842 g         2534 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagggt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactggg
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atccttgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgga agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaatttac ttctggaaca
     8281 ctgtttacca agaggtcatg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcatgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaata
     8521 aagatggaag ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt
//
LOCUS       DAHEYX010000121         8479 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHEYX010000121 DAHEYX010000000
VERSION     DAHEYX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.122"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            105..2714
                     /gene="clpB"
                     /locus_tag="MB219_001876"
     CDS             105..2714
                     /gene="clpB"
                     /locus_tag="MB219_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCC8427590.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2865..3551)
                     /locus_tag="MB219_001877"
     CDS             complement(2865..3551)
                     /locus_tag="MB219_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427591.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3529..4023)
                     /locus_tag="MB219_001878"
     CDS             complement(3529..4023)
                     /locus_tag="MB219_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCC8427592.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4043..5263)
                     /locus_tag="MB219_001879"
     CDS             complement(4043..5263)
                     /locus_tag="MB219_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427593.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5410..7245
                     /gene="lepA"
                     /locus_tag="MB219_001880"
     CDS             5410..7245
                     /gene="lepA"
                     /locus_tag="MB219_001880"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCC8427594.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7339..8466)
                     /locus_tag="MB219_001881"
     CDS             complement(7339..8466)
                     /locus_tag="MB219_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCC8427595.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2725 a         1623 c         1703 g         2428 t
ORIGIN      
        1 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
       61 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
      121 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
      181 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
      241 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
      301 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
      361 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
      421 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
      481 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
      541 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
      601 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
      661 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
      721 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
      781 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
      841 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
      901 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
      961 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
     1021 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
     1081 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
     1141 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
     1201 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
     1261 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
     1321 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
     1381 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
     1441 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
     1501 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
     1561 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
     1621 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
     1681 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
     1741 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
     1801 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
     1861 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
     1921 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
     1981 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
     2041 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
     2101 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
     2161 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
     2221 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
     2281 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
     2341 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
     2401 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
     2461 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
     2521 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
     2581 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
     2641 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
     2701 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
     2761 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
     2821 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
     2881 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
     2941 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
     3001 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
     3061 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
     3121 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
     3181 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
     3241 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
     3301 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
     3361 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
     3421 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
     3481 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
     3541 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
     3601 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
     3661 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
     3721 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
     3781 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
     3841 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
     3901 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
     3961 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
     4021 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
     4081 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
     4141 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
     4201 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
     4261 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
     4321 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
     4381 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
     4441 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
     4501 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
     4561 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
     4621 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
     4681 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
     4741 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
     4801 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
     4861 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
     4921 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
     4981 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
     5041 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
     5101 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
     5161 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
     5221 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
     5281 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
     5341 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
     5401 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
     5461 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
     5521 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
     5581 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
     5641 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
     5701 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
     5761 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
     5821 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
     5881 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
     5941 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
     6001 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
     6061 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
     6121 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
     6181 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
     6241 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
     6301 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
     6361 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
     6421 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
     6481 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
     6541 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
     6601 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
     6661 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
     6721 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
     6781 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
     6841 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
     6901 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
     6961 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
     7021 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
     7081 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
     7141 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
     7201 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
     7261 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtggt
     7321 ttttttcggt ggcaaaaatc aagaattctt tctaagattt tccatgatat cgaccgtttc
     7381 atttctcatc ttatctgtta catgcgagta agtatccatc gtaattgaaa ttctgctgtg
     7441 gcccaatcgt tcagagattt ctttcatttt tgcgccattt tcgagaagaa gtgtagcatg
     7501 agtatgtctg agagaatgga agttaaaaga gagggagagt gcattcgata tcctcctagt
     7561 attccatttc actacacttg gcgtaactag ctcaccgtct tccttcgtac atactgcatt
     7621 tgaatcaatg tagagctttc catacttcat tcgattttct aattgttgtt tcttatgttt
     7681 tttcagaatt gctagcaagg tttgtccaat aaaaatcgtt cgattggaac tgcttgtctt
     7741 tggcgtacca tatacccatg cgccgtcatt ctttaccatt tgtttctcta cagtaattgt
     7801 tccatttgaa aaatcgacat catcccacgt cagaccacaa acttcgccaa cgcgcattcc
     7861 cgtataaaat ccaatattca aaggaatata gaaaggatgt ccttcaggag taatttctag
     7921 catatgatca aagtcctcaa gagaaatgat ttttagatct tttttagtcg ttggtcgtgc
     7981 ttcatatttt ggtattttta catacagcat aggattttgc ttgattaatc cccaaggata
     8041 aaccgccata ttcagcgcat tcttaaggac agagtgagta atagtcattg ttttcttcga
     8101 gtaacccttt ttaaattcag cattgatgaa attttgtaaa agagcagggg agagatccgt
     8161 aagttttttc tttcctaaat aaccgtttat atgatttttg atggtaaatc ggtagttttc
     8221 ataggtattg tattttagat ttagtttaac gtattcctcc atccaaaaat caaggtattg
     8281 ttttactcga gtatccgtac ctaaaaagta ttgtcctgtt tcgtcaatat ctgataaaac
     8341 ttttcgtaaa gcagcttcgg cctctggtcg ggtgtctccg ccaactttct ccactttttt
     8401 tcttgagcca tcatcattga tatcttcgcc gtttgtataa ttaagcgaga caaataaaaa
     8461 agccatttat gttacactc
//
LOCUS       DAHEYX010000122         8442 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHEYX010000122 DAHEYX010000000
VERSION     DAHEYX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.123"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(777..2408)
                     /locus_tag="MB219_001882"
     CDS             complement(777..2408)
                     /locus_tag="MB219_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCC8427596.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="MB219_001883"
     CDS             complement(2422..4095)
                     /locus_tag="MB219_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCC8427597.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="MB219_001884"
     CDS             complement(4108..4425)
                     /locus_tag="MB219_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCC8427598.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="MB219_001885"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="MB219_001885"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCC8427599.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="MB219_001886"
     CDS             6087..6872
                     /locus_tag="MB219_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCC8427600.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="MB219_001887"
     CDS             6875..7567
                     /locus_tag="MB219_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCC8427601.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="MB219_001888"
     CDS             complement(7841..8212)
                     /locus_tag="MB219_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCC8427602.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2604 a         1837 c         1441 g         2560 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa ga
//
LOCUS       DAHEYX010000123         8439 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHEYX010000123 DAHEYX010000000
VERSION     DAHEYX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.124"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..438
                     /locus_tag="MB219_001889"
     CDS             <1..438
                     /locus_tag="MB219_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCC8427603.1"
                     /translation="KESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNC
                     LKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTL
                     RDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            514..732
                     /locus_tag="MB219_001890"
     CDS             514..732
                     /locus_tag="MB219_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427604.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            719..3397
                     /locus_tag="MB219_001891"
     CDS             719..3397
                     /locus_tag="MB219_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427605.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSETVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3388..3585
                     /locus_tag="MB219_001892"
     CDS             3388..3585
                     /locus_tag="MB219_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427606.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3589..4200
                     /gene="mobC"
                     /locus_tag="MB219_001893"
     CDS             3589..4200
                     /gene="mobC"
                     /locus_tag="MB219_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCC8427607.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4185..5576
                     /locus_tag="MB219_001894"
     CDS             4185..5576
                     /locus_tag="MB219_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCC8427608.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5792..6079
                     /locus_tag="MB219_001895"
     CDS             5792..6079
                     /locus_tag="MB219_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427609.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            6099..6593
                     /locus_tag="MB219_001896"
     CDS             6099..6593
                     /locus_tag="MB219_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427610.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6661..6975
                     /locus_tag="MB219_001897"
     CDS             6661..6975
                     /locus_tag="MB219_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427611.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7074..7319
                     /locus_tag="MB219_001898"
     CDS             7074..7319
                     /locus_tag="MB219_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCC8427612.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMDRLQLTRQKN"
     gene            7360..>8439
                     /locus_tag="MB219_001899"
     CDS             join(7360..7636,7636..>8439)
                     /locus_tag="MB219_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCC8427613.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT         3219 a         1181 c         1635 g         2404 t
ORIGIN      
        1 aaagaatcac ataatgatga tcttcttgaa tcactttcaa ctaaaaataa taaattagat
       61 gaagaattga tcgaaacatt tattgaagaa aatcttctta aacaaatatg gggagaaagt
      121 attgtgaatt gcttaaaact ggctagcaat agtgattatc gtcaattcga caactggtac
      181 aaaaaattca aatatgcgat tagatctgct gaaaaagaac aaaaaattca actgaaaatt
      241 atctatgaaa tttgtaataa caagtatttt gtagatcatg ttagagaaca gctatcaatg
      301 actttacgtg acttgatacg aagagcaaaa acagatcatc gtatcaaaca aaaagacgat
      361 tatatctttg cttctctaaa aaataaagcg ttagaactaa ttgaattgca aatcagcgaa
      421 ggaatagata agcaataaga atagaattga gtcaaatgaa atggaggggt atctccactt
      481 catttgagaa ctttgaatat aggaggggtt attttgatta aaaatcgttt aatagatcaa
      541 gtttggtgtc tagatcaaaa tacagatagt ctcgaattaa tcgagactat ttgcttcaac
      601 actatagttc tcgatttaag agctgaaaat gataattgtg taactcatgt cataattaat
      661 caaaaaatgg ctcagaagct ctctgagagc ctcagaaaat gggctaaggg aggtaacgat
      721 gaaaataatt gaaaaaaaga ttgccaataa aaaaataaat tggttggttg ttaattttct
      781 tgttctgata gctatgatta tttttacaag taatattgct catgcgagta ttttcaattg
      841 gtttgatgac acaaaaagtc aaacagagtt tttgaataat tctctttata gaccttacct
      901 aaaaaagtac aatggagatt tccttagtca gttcgggatt tggctcggat gggctgtggt
      961 aaaaggcatg tttactgtga cagattcgat tcaaaatatg attccagatg tgctggattt
     1021 gttcaatttc attgaaagta cagggctaaa taatgtttat caatcagccg taaatacaat
     1081 tgtcattgga ttaatgatcc tttcattgat gtttgttggt tacaaaatga ttactggaaa
     1141 aggaacagtg gatttaaaat cggttggaat gaacgtagtg atgtctatag cattaatact
     1201 tttaatgcca accatgattt ctagtggtat tcaatttgct aaaacatttt ataaggactc
     1261 tactacgatt acaaattcag atgacggagt cgcttggtca ttgataaaac aaggagtaac
     1321 agatttagca tatattaata aaacagatca gtactcaagc attgataaaa cggaggatcg
     1381 aaataattta acacgaaaaa attttcaaca gacggatctc acacaagtgt taacggataa
     1441 agtgattgat aaattggaaa aagaaaatcc aattgcggac aatcttcgtt atgaattagt
     1501 tgaaaattct aataatgaat ttgttgctac aaagttttct gacaacttct tatcaacatt
     1561 ttctgatagc ttgaaatcag gctattaccg ataccaagca aatctatggg ggatttcaat
     1621 tgggttgaca gcgttagcaa tcgcctatgt gttcagcgcg tttgtgatca ttacagcaat
     1681 actagaactt gcatttaaac gtgtgttagg tgttcttgtt tttgcaacag atattgagac
     1741 aggacagcgt tcaaaagttg ttctatcaga tattctacaa tgttacttaa ctgttgggtt
     1801 tcaaggattt gggttatcaa tgtttgctat gtttatcaat tttctgaact caggacaagg
     1861 tatctcaaca aatattttta taaaaacaat tgcttatatc tgtgccgttt ttgttttaat
     1921 caaaggcagt gaaacagtca tgagatactt tggtgttgat attggactca aagaaggcta
     1981 tgggcaactt gcttctgctt ttggaatggg cgcaatgctg tttagaaaag gatcaaatgg
     2041 atttaatcgt gctaaaggaa gcggagaagg agaagatcga aaaccagaaa agaatttcgg
     2101 cgaaactcta tcaaaaaaag ctggcaaaac tggaagagct ttgggatatg cccatgaacg
     2161 tggattatcc ggtttagctt cagatggagc tacaatggct agtgaaagag caacaaaacc
     2221 atttaagagt atgcgtgata tagctaatga taccaagaat aagtttaaag aaggtttaga
     2281 tgatggtaca gtgtccgcta ttaataaaaa tagcaaacct atgttagcta aaaataaaga
     2341 agatgaaact ggaaaatatg ctgattccgt gccaacacgt ctttcagata gaaaagatgg
     2401 tgctaaagtt gaaaatgccg atagaattat gtctagttct gaacgtatgc gtgaagcaat
     2461 gaaaaataat gctgaatcga ataataatcc tgcaagcgca attcagcaaa aagtccaaca
     2521 agatattgaa gaacgtaaaa atgctatgca cggtcaagct aagtctgctg aagaattaat
     2581 taatcagaag cgtcatgaag cgaaatacac gcctgaagca atgaatcgcg aggaaataat
     2641 gttaaggaaa cgtgtagaag ggcggacagg cgctaatatt gacggaaaag aagcgttgac
     2701 caaagaaaga attcaagaag ctaaaaattc aaatacagga ttagaaaaat tagtcaaaga
     2761 aaatccacaa aattctacaa gtagtcatgg tggccgatca gtagatgtcc gagaaaatat
     2821 tcaaggttca ctgaatggtc atgttggacg gaatgttgat gtaagagaga atttacaaag
     2881 ttcttcaaat gctcaaggta ctaagacaat tgatgttcga gaaaatctgc aaagtagcag
     2941 tacaagtcaa ccaaaaacag tagatgttcg agaaaatgta caaaattcta caagtagtca
     3001 agggtttaaa tcagtggatg tccgagagaa tgtgcaaggt agcagtgcaa gtcaaccaaa
     3061 aacagtagat gtccgagaaa atgtacaaag ttctacaagt agtcaaggat tcaaaacagt
     3121 agatgttcgg gagaacattc aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat
     3181 caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat
     3241 tattgatagt gaaatccgtg aaaatgataa gggatcaaaa tcaagacgaa gattcactta
     3301 tgaagacaat gagttattca gagatacact taatgatcct aatccacttt tcgataattt
     3361 attaagaaat aaaagggggg gattgtcatg caattaattc taaagattag tacgcatgat
     3421 ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg caacaaatga aaaaatcacg
     3481 gtcactaatt atattgaact gatgattcaa gaagaattaa agatgaaaaa tgaaatagag
     3541 agcaatttca aatgttgctc tctagaaaaa aatgtgaggg gataatcaat ggatgataaa
     3601 caaaaaatgt acgaaaaaaa tcgcaaacat agtgaggcct taaattttcg tgtcgataaa
     3661 aagacttatc ttgaactgac attgattgca aaacaaaggg gaatttctag agcagacgtt
     3721 tgccgtgagc ggttaaaggg actcaaatat gaaccgccgt tgttagctga agatgatagg
     3781 gtagttttaa ccaaacagtt aaatcaaatt ggtaacaatt tgaatcaagc ggtaaaggca
     3841 ttaaatatca ttgctaaata ttttagagaa gaatctgaag ataaaaaaga aaagagccta
     3901 cattttagtt ttgatgctag ccgttacaac atttttaaag atgaacttgc gaatagcata
     3961 gcaaaaaagt tgatgattag ttatccagat gacatttttt tggagtccaa tcggagtgcc
     4021 ccctcttttt ttaaattatc agaagtagaa aaagaatatt tcgaatattg gatagaaaag
     4081 caaactagac cgggtacgaa agaagagtat tcccaaaaag ttgatcggaa tttaattcac
     4141 tcaattcaga ttattgcaag tttaaaagat gaggtgaaac aactttggga gctactataa
     4201 catcgaaagg acgcaagaaa aatttatcct cgctattaca atatgcagct aatccttcaa
     4261 aaaaagaaaa atgtgttgca gtctcaggaa taaattgtag tgatgatagt gaggaagcac
     4321 tgcaagaaat gaatttagta cgtaccaaga tgaataaatc taaagatata gcacaagcgt
     4381 ttcatgttat tcattcgttt gataagagta cttctaaaga gttaagtatt gataaaatgc
     4441 acgaaatagc tgtcgaattt gcagagaaag cttttccaaa ttcgcaaatc attgttgcaa
     4501 gtcacaatga taaagatcat tttcatagtc atcttgtcat taataatatt aacatggaaa
     4561 catgtaaaag gttgcgtatt gaccctaaag atttagaact aatgcgaaaa attaacgatc
     4621 aagtcctcaa tgaacatggt cttgaatcag tgaataatga gtactatgaa aacttagaaa
     4681 agagaaccaa tctttattca aacgatcctt ctaagatgaa atatggaaaa attaattatc
     4741 aagagtttgt gcaaaatgcg atcgaagcaa cgttggaaaa atgcgaagtg aattcttttg
     4801 aagatttttc ggatcatttg gcaaaagaat ataacattga agtttatcaa ttttcaaaaa
     4861 caaataacaa acttggctat gttctttaca aacaaggtgt aaatttctat gagcgtagag
     4921 gtgagttaat tactttagga agagataaac aatctagaaa agaaaaaagt cagtatgtcg
     4981 aacgtacttt tagtgcaaga aagttaggga aaagatattc atttgaatct gtttctgaac
     5041 aacttgaaaa gaatttagaa gaatcacata taaaaaattt ttttaatatg gaaagagagc
     5101 aagttatgtc gctaattagt ggagtaaatt acaaaactgg tgagttaaat gtaccgtttg
     5161 tggaattaga ttccatcgca caaaagcaat ttagcgaccg tgaaggcgat gaaaaattca
     5221 ttaaatatga tgacttcttg cgagaggtag acaagcattt tgtaaataca atgtctttag
     5281 aagatgatca agaatacacg attaaacttc atacggatgt gaatcctcaa actttacgta
     5341 gtgcgaaaag taaaacaatt tctattaaaa agactgatca gaaagagtta tttctaccgg
     5401 caaagggttt tgaaaattgt tctcatacac cttctgctaa acagattcac tatcaaatca
     5461 gtcgcataat gaatgacgat gtaaaaatgt atcgtgctaa aaaggattat gaattcacac
     5521 aacaaatgat tgaaaatcag caaaaaaggc aacaaaattt tgaaatggaa ttctgaatgg
     5581 gcttgttagt ggactttcca gttttttgcc tatgaagagt tgcttggaaa gaagcagttt
     5641 ttaggacata cgccagctac ccatctactt gccagtcaat ccgccccgct cacgctaggc
     5701 tactttctgg caagtcagat ggtgggtaaa tctctgaggg agcaagctcc ctacagactc
     5761 cctcccaaaa aaagaaaggt ggaagaaaaa aatgaactat ccatatttta aagtctcggc
     5821 ttcagaagaa actaaagaaa tattcaacaa tttttacaac caaaacaaag gagtttttgg
     5881 ttcaaaggca aatatgtttc gtgttatggt ttcgaattta ccagtgttag ctagtccatc
     5941 aaataataaa tttaatgatc ctgaaagcat taaatttgaa caaaaaattt cagaactaga
     6001 atcaatgata agtaatgaag taattgaaaa gctggatgac attgatcaaa agttatcgta
     6061 ttctctaaaa aacaaataaa aaacggagga gaaaaaagat gtttaaagtt tacttcccgt
     6121 ttcagtggct cgttaaagga attcttttag tgctactagg attgattctt tttggaagtt
     6181 atctaacaat taaagccaga cattttccag aagaaaagcc aataaaaatt gtaacaaaag
     6241 atttaaaaat aggtgaaact tatcaggcag atggggttga actaacattt aaaaatattg
     6301 aagtgaccga aaaaagaaat gaatttgcac aacatgatcg tgtattacaa gtgacctata
     6361 cacttataaa tgatacagaa aatccgatac gtccacaaca atggtcgatt gaaaataagg
     6421 atggtgaaga actttatgag tatgatatga tattggataa tccagagtat attgaaagtg
     6481 gcaaagaaga aacagtaaat tactattatg aattaccgat tgaaaggcag gactaccaaa
     6541 tcgtagtacg tagagagaat ggttacatag ttgggttgcc atttacttta taagttttat
     6601 tttgttagta gtttttaagg taatatcagt tttgtgattt ttgaaaatgg aactgggaga
     6661 atggctgagg aaaataaagt tgttccgttt gaaacattag tgataaataa ggaagagttt
     6721 gagcaacttt ttcttgattt ttttattcaa tatgacatga tttatgattt tagttcgtca
     6781 ctagatttat ttgaattaga agcagaaata atagaaaaca gtcgagagaa gaatggtaaa
     6841 atcattattt cagtagaaaa attcaatgaa ttattctcac aaacatttga aatagatcca
     6901 gactattatc aaccacaaga agtttttcga gagtttagaa ataagattat tggttttagt
     6961 caaactacaa aatgaaagct taataaagca agtgttgata gaattattcc tcaacgcttg
     7021 ctttatttta tgggcacttc ataatagagg tatagagaag agaggtattc attttggata
     7081 ctattactcc aacaaacttc aaagaaaata taggtaaagt gttgcaagaa gtagttcatt
     7141 caaattctga aatagaaatt actagggatt ctaaaaatgg attgaacgat ggcgtcatta
     7201 tgatttctaa gagaaaatgg gagttgttac aagaagaact ttatttacaa cgaacgggta
     7261 catgggctta tgtatctgat ttaatggata ggctacaatt aactagacag aaaaattaag
     7321 gtgtgtagac tagaagaaaa cataccagga ggaattttta tgtctaagag aacacgaaga
     7381 actttttcac aagaattcaa gcaacaaatc gtcaatcttt acttagctgg aaagccacgt
     7441 gtagaaatca ttcgagaata tgaactaacg gcttcagcat ttgacaaatg ggtaaagcaa
     7501 tctaaaacga gtggttcatt caaagaaaaa gataatctta cgcctgaaca aaaagaattg
     7561 ttagaactac gtaaaagaaa ccagcaatta gaaatggaaa atgatatttt aaagcaagca
     7621 gcgctgatat tcggacgaag agacaagtaa tcgatgcgaa taagcatctt taccctatat
     7681 cagcgatgtg cagaatatta ggtctatcac gtcagtccta ttattatcaa tcaaaaccaa
     7741 agaaagacga atcagaactt gaagaagtag tcgctgaaga atttatccgc agccgaaagg
     7801 cctacggctc aagaaaaata aaaaaagcct tatcaaaacg aggcattcag atcagccgac
     7861 gaaaaattag tagaatcatg aaaaatagag gattaaaatc gagctatact gttgcttatt
     7921 ttaaagtaca tcattctact tgcaatgaag ccaaaacgac aaacgtattg aatcgtaaat
     7981 tcttaagaga caacccatta gaagcgatcg taacagactt gacttatgta cgagtcggga
     8041 aaaaatggaa ttatgtctgt ttcattttgg atctgttcaa tcgagaaatt ctcggctatt
     8101 cttgtggaga acataaagat gccgttctag taaaaaaagc atttagccgt atcaaacaac
     8161 ctctgacaga ggttgagatt tttcatactg atcgtggaaa agagtttgat aaccaagcta
     8221 ttgatgaatt attaacaact tttgacatca atcgatcatt gagtcataaa ggctgtcctt
     8281 ttgataatgc cgtagctgaa tcaacttata agtcgttgaa agtagaattt gtctatcaat
     8341 acacatttga aaccttacaa caattggatt tggagttatt tgactatgtc aattggtgga
     8401 accaccttcg gttgcacggt acacttggct acgagacac
//
LOCUS       DAHEYX010000124         8338 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHEYX010000124 DAHEYX010000000
VERSION     DAHEYX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.125"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     regulatory      187..266
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            264..1457
                     /locus_tag="MB219_001900"
     CDS             264..1457
                     /locus_tag="MB219_001900"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCC8427614.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="MB219_001901"
     CDS             1587..3059
                     /locus_tag="MB219_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8427615.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="MB219_001902"
     CDS             3142..3930
                     /locus_tag="MB219_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCC8427616.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="MB219_001903"
     CDS             complement(3997..4665)
                     /locus_tag="MB219_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCC8427617.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="MB219_001904"
     CDS             4780..6492
                     /locus_tag="MB219_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427618.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="MB219_001905"
     CDS             6495..8267
                     /locus_tag="MB219_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427619.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2553 a         1391 c         1829 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggcagaa
//
LOCUS       DAHEYX010000125         8338 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHEYX010000125 DAHEYX010000000
VERSION     DAHEYX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.126"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            15..794
                     /locus_tag="MB219_001906"
     CDS             15..794
                     /locus_tag="MB219_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427620.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            840..1784
                     /locus_tag="MB219_001907"
     CDS             840..1784
                     /locus_tag="MB219_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCC8427621.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1800..2579
                     /locus_tag="MB219_001908"
     CDS             1800..2579
                     /locus_tag="MB219_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCC8427622.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            2591..3289
                     /locus_tag="MB219_001909"
     CDS             2591..3289
                     /locus_tag="MB219_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCC8427623.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3317..4081
                     /locus_tag="MB219_001910"
     CDS             3317..4081
                     /locus_tag="MB219_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCC8427624.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4095..5914
                     /locus_tag="MB219_001911"
                     /pseudo
     CDS             4095..5914
                     /locus_tag="MB219_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            5925..6557
                     /locus_tag="MB219_001912"
     CDS             5925..6557
                     /locus_tag="MB219_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCC8427625.1"
                     /translation="MKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSLKMKNQELLG
                     KPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVNISGHVILGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            6595..7677
                     /locus_tag="MB219_001913"
     CDS             6595..7677
                     /locus_tag="MB219_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCC8427626.1"
                     /translation="MSEEGYEQEYVKEAFDTNWVAPLGANVNGFEKELAKYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPVFVDSDYETWNMK
                     PEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKNELSKLSNIQFMPENNWNEPNYW
                     LSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGDDVAETLFDLGVC
                     LPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            7667..8287
                     /locus_tag="MB219_001914"
     CDS             7667..8287
                     /locus_tag="MB219_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCC8427627.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
BASE COUNT         2963 a         1401 c         1719 g         2255 t
ORIGIN      
        1 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
       61 caggtgctat tgtgatctgt ttgtttggtt accatatcaa taatcagcgg caacatcagc
      121 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      181 cgaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      241 cagaagaaac aattacaagt ttgtccaata agctatcctc tgtcaaactt tcagcagatg
      301 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      361 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      421 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      481 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      541 atagtccttg gaaaacggtt gttatacaat atctaggatt cgctgatacc cagatcgcgc
      601 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      661 ccgctactta cgatcaatat ttgacggcgc tcagtcaaat agagcagatc cggaatgaaa
      721 aaataagcgt agcatacgca acaaaagcac aaactgttgc tcagcaaatg ggctatagca
      781 atataagcta ttgacagtta aagcgttgat tcaagcaagg ttagcaaacg gaggaaacta
      841 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
      901 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
      961 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1021 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1081 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1141 acaaacagac aacgatcgtc agcattcctc gagatacgta tgtcgacatc gtaggtagtg
     1201 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1261 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1321 taaaagaact tgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1381 aagatggcta tgactttaca atcggtaaaa ttactttgaa cggggatcag gcattatctt
     1441 attctcgtat gcgttatgaa gatccaaacg gtgactatgg tagacaagag cgtcaaagaa
     1501 agatcgtaga aggtgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1561 ccgttttgaa tgctctagag tcgaatatga aaacagacat gagctttgat gaaatgaaaa
     1621 aaattgcttt agattatcgg gaggcatttg gtacgatcaa gcaagatcag ctgcaaggag
     1681 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     1741 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     1801 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaacaa
     1861 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     1921 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacacagcaa
     1981 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2041 gtgatttagt actaaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2101 ctgctggaca aatcaaagat gcgatctccg tcaatcagtc acaaaattca caaatgttct
     2161 cgatccaggc aataagcacg aatgcagtga ctgctcaaca gatcgctaat acaacttcac
     2221 aagttttcca agaaaacgca aaagacgtga tgaatgtcga taaaatctcg attatttcag
     2281 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaatcta gtgattggct
     2341 tagtattagg aatgatggta ggcgtaggtt tggcattctt attcgaatta cttgatcgga
     2401 cagtgaaaga tgaaaaatat gtgacggata cattaggatt cccaatctta ggaacagttc
     2461 cagaaatgtc agcgaaagaa ttgaacgctg gtatccgtaa aaaaagaaca gctcctgcac
     2521 gaccaaaagc tacaaacgat aaaacagccc catcacgcag aagccgctca agagtctagg
     2581 aggaagaaaa atggcacgaa cacagaaaca aaaagcaaca caaactcatg cagtcagttt
     2641 gatcacatta acaaacccat cttcaccaat cgccgaacaa tatcgtacga tccggacgaa
     2701 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgtcgtga cttcatcagg
     2761 tcctggagaa gggaaatcca ccactgcagc aaacattgca gtcgtttttg ctaaatccgg
     2821 tcaacgagtt ctactagtcg atgcagattt acgtaaaccc gtcgtctata agacattcca
     2881 gttgaacaat gccagtggat taagtacagc acttagttca tcaggaagtg tagcagatgt
     2941 gatccaacga acgccagtcg agaacttgtc gattttgcct agtggaccta agccacccaa
     3001 cccatcagaa ttgttaagct cgccaagaat ggatcagatc ctagctgaag caagacagtt
     3061 atttgacgtg gtgatattcg atatgccacc agtagtagcc gtaacagatg cacagatcat
     3121 gtcttcaaaa acagatggca ctttattggt cgtacgtgaa aatatgtcac gtaaagaatc
     3181 cttgactaaa gcaaaagaac tgctagagat ggtacaagca cgagtcttag gtgtcgtata
     3241 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3301 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3361 gtcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3421 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3481 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3541 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3601 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3661 gcctttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga caccagtgat
     3721 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     3781 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     3841 catccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     3901 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     3961 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4021 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4081 gggggagtta aaaagtgttt atattaacaa gaagaagaaa aattgccaca ttggttgttg
     4141 tggacagctt gttattaatc gctgcaaata ttgcagcagc taaattcatg aagccgtttg
     4201 tatctatacc aatggatctt attttaatct ctatcggttt gtcgattggt ttttatttat
     4261 tttatggtag tttattcaaa gttttcactc ggatcaatcg ctatacaaat ttaagagaga
     4321 tcattgcaat ttttagttca ctatctgcaa gtgctgcctc aagtatcttc atactgctgt
     4381 ttattaatcg gcgatacagt ttacggttag ttatctttac ttatctgctt tcgctcttat
     4441 tgatcatcgg tagccgattg atctggcgta tctacatcga aaaaaaaaaa atatgagata
     4501 tgtatcagca gattctgcta aaaatacatt gattgttggt gctggcgaag gcgggcgtat
     4561 tttatacaac agctttcttg gatccaaaac ggctcaagat atccacgttg tcgggtttgt
     4621 agatgatgat ccaaataaaa gaaatacgta tctttcagga aaaaaagtat taggtgcatt
     4681 aaaagatatt ccggaattga ttgaaaaata tgacattcaa atggtaacga tcgctatccc
     4741 ttccttatca agaaaaaaat tacgacgaat cttcgagtta gtcgaatccg ctcgtgtgaa
     4801 agtcaacacg atgccttcta tagaagaact tgcttcaggg aaaattagcg tatcaaaatt
     4861 aaaaacaatt gacgtagtgg atttactggg aagagatgag gtaaagctgg atattgaatc
     4921 catcaaagat cagatcacag acaaagtcat tcttgtcacc ggcgcaggag gatcgatcgg
     4981 ttcagaaatc tgtcggcaaa tcattcaatt caatcctgca aagctattgc ttttaggaca
     5041 tggggagaac tcgatctact tgattgaccg tgagttacgc acgcatcatc aaaattgtcc
     5101 tacagaaatc gtaccgatca tcgcagatat ccaagatcgc gagaaaatca atgagatcat
     5161 ggaacaatat catccagata tcgtctacca tgcagctgct cataaacatg taccactgat
     5221 ggaatataat ccaaaagaag cagtcaaaaa caatattttc ggtacaaaaa atgtagcaga
     5281 agcagctaaa gcagcaaaag tgaaaaactt cgtcatggta tcgacagata aagctaacaa
     5341 tcctccaaat gtcatgggtt caacaaaacg aatcgctgaa atgattgtga ctggattgaa
     5401 tgaagaaggc tgtacgaaat tctcagctgt cagatttggg aatgtccttg gttctcgcgg
     5461 tagtgtgatt ccagtattca gagaacaaat tgctcaaggt ggtccaatca ctgtaacaga
     5521 ctttcgtatg actcgatact ttatgacgat tcctgaagct agtcgtttag ttattcaatc
     5581 tggcgcatta gcaaaaggtg gcgaaatttt tattctagat atgagtgaac cagtgaagat
     5641 tgtagaccta gcgaaaaaca tgatccgatt gagcgggtat agcgaagacg aaatcgaaat
     5701 cattgaaaca ggtatccgtc caggggaaaa actatatgaa gaactattgt tggataagga
     5761 aagaaatgat gaagcagttt atgagaaaat ctttgtagga aacatcaaag gctactcaat
     5821 acaaaaagtg atggattttg ttaagagcct gccgcaagac gatgaacaat tggctaaaga
     5881 cattgtcact tttgcaaatg cttcgaataa gtagggggag taaagtgaaa gcaattatta
     5941 taggagctgg tggatttggc aaagagattg cttttctatt acaaagtgtc tctagatatg
     6001 aattgattgg ttttgtcgat gattcgttaa aaatgaaaaa tcaagaatta ttgggaaaac
     6061 ctgtattagg aacaatagat tcattaatag aattagaaga agagactgct atttttctgg
     6121 gaattgcttc gccagatatc aaggagaaaa tatatcaaaa gataaataaa aataataaac
     6181 tgattttccc taatttagtt gcaccaagtg cactagtggg cataaatgtt cagttaggga
     6241 ttggcaatat tttgatgccc tatacgactt atactgctga cgtagtttta ggaaatttca
     6301 atatgatcaa tatcggctct actatcggac atgatgctca aatcggtaat tataattcaa
     6361 tttttccaag tgtaaatatt tcaggacatg ttatattagg agataaaaat gaaattggag
     6421 taggtactaa aataatacaa aatctaagaa ttggaaatat gaatattata ggtgcaggaa
     6481 gtgtagtaat aaaaaatatt aataataata ctaaaaatgt tggcgtgcct accaaagtaa
     6541 tagaaagatg ggattaagtt ggaaaataaa cgaatattat tagcatctcc tcatatgagt
     6601 gaggaaggat atgaacaaga gtatgtaaaa gaagcatttg atacaaattg ggttgctcct
     6661 ttaggtgcga acgtgaatgg ttttgagaaa gaattagcaa aatacgtggg agctagtcat
     6721 gctgccgcat tgtcttctgg aacagcagct atacatttag ctttgaaagc ggctggggtc
     6781 ggtaaagatg acatagtgat ttgtcagtcc ttgacgttct cagctactgc taatccaatt
     6841 ctctatcaag gagctatacc tgtatttgtt gatagtgatt acgaaacttg gaatatgaaa
     6901 cctgaacatt tgcgaacagc actagaaaaa tatcccaaca caaaggctgt attagtagta
     6961 catttgtacg gtttatcagc taatttagac gaaattacta aaatatgcaa agaatatgat
     7021 gttactctaa ttgaagatgc tgcagaatca ttaggaacaa cgtataaagg aaaatatact
     7081 ggtacatttg gcgactatgg aatttattct tttaatggaa ataagataat cacaacttca
     7141 ggtggcggaa tgcttgtatc taatgacaaa gaaaaggtgg aaaaagtgag attttgggct
     7201 acacaatctc gtgagaaggc acgccattat caacacaatg aaattggcta taattatcga
     7261 atgagcaata tcgttgcagg tatcggtcga ggacaattga aagttttaaa tgaaagaatt
     7321 gagaaaaaac gatatattca tagtttttat aaaaacgaat tgtctaaatt atcaaatata
     7381 caattcatgc cagaaaataa ttggaatgaa ccaaattatt ggttaagtgt cataacaata
     7441 aagggaataa atacgaatca tcttattgat gagttagata aaaataatat agaagcccgc
     7501 ccagtatgga aaccaatgca tttacaacct gttttcaaaa attatgatta tattggcgat
     7561 gatgtagcag aaactttatt tgacttagga gtatgtttac catcagatac aaagatgaca
     7621 gatgaagatt taaaatatgt agtatctacg attaaaagag tgatcaatga tgcgtaaaaa
     7681 aactttctat cagctttttg ggaaaagaat tttagatatc ctattgtcag gaattgctct
     7741 catcgtttta tcgccgataa tcttaattgt aggtttttta gtaagaatta aacttggttc
     7801 tccaattatc ttcaaacaag aacgaccagg aaaaagtgaa aaaatttttt ccatgtataa
     7861 atttcgtaca atgactgacg aaagagacca caatggagaa tatttacctg atgaaattcg
     7921 actaactaaa tttgggaaaa tgcttagagc aacaagtctg gatgagctac ccgaactttg
     7981 gaatattttg aaaggtgata tgtctatcgt aggacctcgt ccattattag tggagtattt
     8041 gccactatat agtgagaagc aacggaaaag acacaacgta agaccaggat taactggact
     8101 ggctcaggtc aatggaagaa atgctattag ttgggaagaa aagtttgatt tagatgttta
     8161 ttatgttgat aaaattagtt tctttaatga tcttattatt ataattcaga catgtaaaaa
     8221 agtaatcaag aaagagaata ttaatacaat caataacaat accccagaaa aatttaaagg
     8281 gagttaaata cagcagctta atgcaagttt tgtgacttgc attatattta ggtataaa
//
LOCUS       DAHEYX010000126         8136 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHEYX010000126 DAHEYX010000000
VERSION     DAHEYX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.127"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(128..976)
                     /locus_tag="MB219_001915"
     CDS             complement(128..976)
                     /locus_tag="MB219_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCC8427628.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1200..3140)
                     /gene="thrS"
                     /locus_tag="MB219_001916"
     CDS             complement(1200..3140)
                     /gene="thrS"
                     /locus_tag="MB219_001916"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCC8427629.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3477..3656)
                     /locus_tag="MB219_001917"
     CDS             complement(3477..3656)
                     /locus_tag="MB219_001917"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCC8427630.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3703..5526)
                     /gene="cadA"
                     /locus_tag="MB219_001918"
     CDS             complement(3703..5526)
                     /gene="cadA"
                     /locus_tag="MB219_001918"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCC8427631.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5804..8083
                     /locus_tag="MB219_001919"
     CDS             5804..8083
                     /locus_tag="MB219_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8427632.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2391 a         1717 c         1465 g         2563 t
ORIGIN      
        1 attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa cactttgatg
       61 gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat aaaaaatagt
      121 aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt tttttcactt
      181 tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg atcgtagcaa
      241 attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt ggtctagatg
      301 tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc gtcaacgcaa
      361 tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct gcacttctac
      421 ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt tgcgggagca
      481 agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat ttttgcagaa
      541 tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc tctgccgatt
      601 ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact gtttctttta
      661 tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac tcatttgctt
      721 ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc gtgattttga
      781 agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc ggcgcactca
      841 acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca atattaccga
      901 taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata ctttcaatac
      961 gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta agtataccat
     1021 atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat ccgcccaaca
     1081 gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt tgaggaatac
     1141 ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa atttacctat
     1201 tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa gatattcaaa
     1261 tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc atcttccaat
     1321 tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc acgtatctta
     1381 tagcccattt tttcattacg atcatcaact tctacacgca accccttcat ttgcaaacgt
     1441 tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat gattgttgct
     1501 tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa ataagcgaca
     1561 aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg cgtattttcc
     1621 ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc taattgtata
     1681 gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag ttttggtccg
     1741 taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc catagcggct
     1801 ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt atctgtattg
     1861 tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc agcatatact
     1921 gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg acgaacaaat
     1981 gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa agcacctgat
     2041 ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc acgataagaa
     2101 tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg aagaacaagc
     2161 atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg atcccagtgt
     2221 ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg ttggtaacct
     2281 agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt tgcccctttt
     2341 ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa gtcaagttct
     2401 tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat atattctttc
     2461 aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg attgtttgag
     2521 tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat acggccagtg
     2581 gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata agcagtaatc
     2641 acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact tgcaaaaatt
     2701 tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc tttgacgatt
     2761 ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt ttgtccatta
     2821 tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg gatatttgga
     2881 tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac taagccaagt
     2941 gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat tggacgagtc
     3001 aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa gctttttgaa
     3061 atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac tgcaccatct
     3121 ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg atcagatcat
     3181 caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg acgaaaagtt
     3241 cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa cggtccgacc
     3301 gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc tggaaaagtt
     3361 tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt tctcatcaat
     3421 caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa ctaaacttat
     3481 tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat gatgacatgg
     3541 atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc tttcatcata
     3601 ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa tggcataatg
     3661 ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta tgtttgtctt
     3721 gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac tcaaaataga
     3781 gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta gtcccgtact
     3841 tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact gtttcgctat
     3901 tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat catctttcaa
     3961 gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca ttgcaatacc
     4021 gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca tcgcaacagg
     4081 tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca agctacgggc
     4141 atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag atccatcccc
     4201 ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg tctttgctgc
     4261 ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat caattgttac
     4321 atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg cacgtccaat
     4381 ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt ctttcagttg
     4441 actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag ctaagatatg
     4501 gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt ttattccatt
     4561 gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg ttttatcaaa
     4621 agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta caacgatccc
     4681 gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta gaatcagtgg
     4741 acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg agaaccacca
     4801 agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac gatcagctaa
     4861 acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa tcatttgata
     4921 ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc catttaaaga
     4981 ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac ctgtcaaaga
     5041 agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta caagttcgcc
     5101 aggcttgact agtaactggt catcaatatt tatttcatcg attactatat cctgcaattt
     5161 tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact gcagttcttt
     5221 acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca tgatcaatat
     5281 catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg taatcgctaa
     5341 tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa acatcaagaa
     5401 ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct ggtgaaaaac
     5461 aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt ttttgaaatg
     5521 tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa atttccagct
     5581 gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata ggccttaatt
     5641 gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac aaaaatattc
     5701 agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa aaactatgct
     5761 atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg agaaacaact
     5821 agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa atatgctgga
     5881 tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca cagctaatca
     5941 caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat atcgccaaca
     6001 tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac gcctgcaaac
     6061 gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag aagaccgtga
     6121 aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag aaacaatcgt
     6181 tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg gtcccgcatt
     6241 ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac aatttaaaat
     6301 ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg atgacttttt
     6361 gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct ctacgataca
     6421 aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga ttgaaggaat
     6481 cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt tgtatcataa
     6541 tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt tgttttctga
     6601 ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac agacattcaa
     6661 aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc aacaagaaag
     6721 tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat tgaccttagt
     6781 cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt tccgtgatat
     6841 gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt ataaagaaac
     6901 aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc tcctgaaaaa
     6961 attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc tggcggaaga
     7021 acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac aaaaggagcg
     7081 ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc gaatccatcg
     7141 agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact ttctacaaac
     7201 tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc aggaacattt
     7261 gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg ctgtactatt
     7321 ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc gctatatctt
     7381 tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag ccttgttccc
     7441 aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa atgaaacgat
     7501 cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc aattaacgac
     7561 aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg tgaaagaaca
     7621 tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag gaacaacaga
     7681 tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa aagcccggta
     7741 ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt atgctcaact
     7801 gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact ttatgaaacg
     7861 ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc gagttttctt
     7921 gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc tttacaccat
     7981 gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa gtcctttgtt
     8041 aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa gacaactgct
     8101 atcattaatg ttagcagttg tctttttatt tacaat
//
LOCUS       DAHEYX010000127         8068 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHEYX010000127 DAHEYX010000000
VERSION     DAHEYX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.128"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..884
                     /locus_tag="MB219_001920"
     CDS             <1..884
                     /locus_tag="MB219_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCC8427633.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="MB219_001921"
     CDS             932..3061
                     /locus_tag="MB219_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCC8427634.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="MB219_001922"
     CDS             3036..3725
                     /locus_tag="MB219_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427635.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="MB219_001923"
     CDS             complement(4133..4819)
                     /locus_tag="MB219_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8427636.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="MB219_001924"
     CDS             complement(4955..5149)
                     /locus_tag="MB219_001924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427637.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="MB219_001925"
     CDS             complement(5199..5639)
                     /locus_tag="MB219_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCC8427638.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="MB219_001926"
     CDS             complement(5643..6488)
                     /locus_tag="MB219_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCC8427639.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="MB219_001927"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="MB219_001927"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCC8427640.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1514 c         1714 g         2359 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga atcggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAHEYX010000128         8051 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHEYX010000128 DAHEYX010000000
VERSION     DAHEYX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.129"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            476..1339
                     /locus_tag="MB219_001928"
     CDS             476..1339
                     /locus_tag="MB219_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCC8427641.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="MB219_001929"
     CDS             complement(1468..3090)
                     /locus_tag="MB219_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCC8427642.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="MB219_001930"
     CDS             3339..3860
                     /locus_tag="MB219_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCC8427643.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="MB219_001931"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="MB219_001931"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCC8427644.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="MB219_001932"
     CDS             5941..6759
                     /locus_tag="MB219_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCC8427645.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="MB219_001933"
     CDS             complement(6862..7059)
                     /locus_tag="MB219_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427646.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="MB219_001934"
     CDS             7228..7806
                     /locus_tag="MB219_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCC8427647.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="MB219_001935"
     CDS             7901..8038
                     /locus_tag="MB219_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427648.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2404 a         1466 c         1591 g         2590 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt a
//
LOCUS       DAHEYX010000129         8049 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHEYX010000129 DAHEYX010000000
VERSION     DAHEYX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.130"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            285..1823
                     /locus_tag="MB219_001936"
     CDS             285..1823
                     /locus_tag="MB219_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCC8427649.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1820..2785
                     /locus_tag="MB219_001937"
     CDS             1820..2785
                     /locus_tag="MB219_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427650.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2772..4094
                     /locus_tag="MB219_001938"
     CDS             2772..4094
                     /locus_tag="MB219_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCC8427651.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4157..4261
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4335..5078
                     /locus_tag="MB219_001939"
     CDS             4335..5078
                     /locus_tag="MB219_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427652.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5093..6085
                     /locus_tag="MB219_001940"
     CDS             5093..6085
                     /locus_tag="MB219_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8427653.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6097..6834
                     /locus_tag="MB219_001941"
     CDS             6097..6834
                     /locus_tag="MB219_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427654.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6824..7546
                     /locus_tag="MB219_001942"
     CDS             6824..7546
                     /locus_tag="MB219_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCC8427655.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7630..>8049)
                     /locus_tag="MB219_001943"
     CDS             complement(7630..>8049)
                     /locus_tag="MB219_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCC8427656.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2573 a         1392 c         1754 g         2330 t
ORIGIN      
        1 tactttattg gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc
       61 tgattccaag tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac
      121 caaaaagttg atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat
      181 ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag
      241 gaaagaaatg tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac
      301 aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt
      361 ctctcatttc tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca
      421 atcataaaaa aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt
      481 cacttgcctt attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga
      541 ttgcacttgt tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg
      601 aaattgaccc gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct
      661 tgctgaaagt agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct
      721 tagttttaat catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga
      781 catttatttt gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca
      841 gttctgcctt gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac
      901 ctcgatattc catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat
      961 atcagtctgc cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac
     1021 ctgaaatcct gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg
     1081 aattaaaacc gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa
     1141 tcgatatcta tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag
     1201 caatgaaaag tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga
     1261 ataagcaatt cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg
     1321 cagtcaagag atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta
     1381 tctctgatca gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata
     1441 aaggaatcca agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt
     1501 tgcgttacca agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata
     1561 gccagctcaa ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt
     1621 atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag
     1681 cactgaataa attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag
     1741 tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat
     1801 tagtaaagga ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta
     1861 tgatggaaag tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt
     1921 tttggttggt ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc
     1981 taaaccatca gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca
     2041 gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac
     2101 agtcaagcaa aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc
     2161 agaccgtgta gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg
     2221 tatgcctaga gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc
     2281 tgcaagccca gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag
     2341 aaactcatta caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt
     2401 cgttacgcac aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga
     2461 tggagaaatt atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta
     2521 cgttcggcat ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt
     2581 cgtcaatgga tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga
     2641 tacgcctatg aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga
     2701 aaagcaaaga agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga
     2761 ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa
     2821 gtgctgctta tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat
     2881 ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag
     2941 aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca
     3001 actgggaaga acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa
     3061 aaaaacaatt agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg
     3121 attcatctat tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg
     3181 ggcaaagacc tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact
     3241 ttttatcatt gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt
     3301 ttgagctagc tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc
     3361 aacggccatt aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag
     3421 catctggcat tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc
     3481 gctatcgttt agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg
     3541 ggagacagcc gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac
     3601 acggaattgc agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag
     3661 atattgtttc aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg
     3721 tagctaagcg gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga
     3781 tcgtctacgc cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt
     3841 cagatcaagt ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac
     3901 cagttgctaa ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga
     3961 ttagcgagca agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga
     4021 ctaaaaaaga gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct
     4081 atttattgaa ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta
     4141 cactttaaaa aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt
     4201 gaacttagac ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc
     4261 ttgaagcggg ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga
     4321 aaggaggtaa caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa
     4381 ttatttggca gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg
     4441 ctgctgtatt ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga
     4501 cattaaatga agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct
     4561 tggcaatgga ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac
     4621 aaatgttgcc aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc
     4681 ttccaaaagt gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg
     4741 atacattgag gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata
     4801 cagggaagat ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt
     4861 taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc
     4921 aagcaggctt agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt
     4981 ttgctgcgat catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat
     5041 tgaaaaaagg gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa
     5101 attatggctt ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac
     5161 aagtagcaaa caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc
     5221 gaatactaat catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg
     5281 tttaaatgtc caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc
     5341 ggacaaagca caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg
     5401 acagaatata ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc
     5461 caccctatca gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg
     5521 ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga
     5581 cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc
     5641 ctcagatatc caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat
     5701 tgaactaaac tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt
     5761 aattattacg aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga
     5821 tgccacgaaa aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt
     5881 gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc
     5941 aaaaaattat acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa
     6001 ttatacagct tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact
     6061 attcactaat cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg
     6121 aatgtagtca ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat
     6181 acctttacat cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta
     6241 acaggagtcc agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt
     6301 ctccatacag catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc
     6361 tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta
     6421 agggattatc tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta
     6481 tctggcggga tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac
     6541 ttgcttcttg atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac
     6601 tggatgttga agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc
     6661 gaagaagcaa tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg
     6721 attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca
     6781 gagttgaagc aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca
     6841 ttgtcattta gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac
     6901 gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa
     6961 gactcaagcc ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca
     7021 agttgagcct tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg
     7081 gacgaatgta ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc
     7141 cataaatgag aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta
     7201 tatccaagct taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca
     7261 agactatatt gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc
     7321 agtctggcaa ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact
     7381 acatgcagca gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct
     7441 tgaagaacag ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa
     7501 aaatacttat caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac
     7561 acaactagtg tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat
     7621 aaatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc
     7681 ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc
     7741 atttaagtaa gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta
     7801 agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt
     7861 agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt
     7921 ttccgtttga accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac
     7981 ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg
     8041 tcctgagta
//
LOCUS       DAHEYX010000130         7982 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHEYX010000130 DAHEYX010000000
VERSION     DAHEYX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.131"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            109..1200
                     /gene="phnW"
                     /locus_tag="MB219_001944"
     CDS             109..1200
                     /gene="phnW"
                     /locus_tag="MB219_001944"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCC8427657.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1193..1321
                     /locus_tag="MB219_001945"
     CDS             1193..1321
                     /locus_tag="MB219_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCC8427658.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1325..1870
                     /locus_tag="MB219_001946"
     CDS             1325..1870
                     /locus_tag="MB219_001946"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCC8427659.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2324..4015)
                     /locus_tag="MB219_001947"
     CDS             complement(2324..4015)
                     /locus_tag="MB219_001947"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCC8427660.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4435..5382)
                     /gene="arcC"
                     /locus_tag="MB219_001948"
     CDS             complement(4435..5382)
                     /gene="arcC"
                     /locus_tag="MB219_001948"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCC8427661.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5497..6516)
                     /gene="argF"
                     /locus_tag="MB219_001949"
     CDS             complement(5497..6516)
                     /gene="argF"
                     /locus_tag="MB219_001949"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCC8427662.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6607..7836)
                     /gene="arcA"
                     /locus_tag="MB219_001950"
     CDS             complement(6607..7836)
                     /gene="arcA"
                     /locus_tag="MB219_001950"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCC8427663.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2274 a         1624 c         1470 g         2614 t
ORIGIN      
        1 tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc
       61 agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta
      121 ttaacttctg gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat
      181 tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa attattggat
      241 cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag cagctccttc
      301 ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca
      361 aacggcgcat atagacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc
      421 gtttatgagg tatcggaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa
      481 gagaatccgt ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg
      541 aataatttgg aagagattgc tgctgtcaca caagaaagag atcttttgtt tatagtggac
      601 gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg aactagggat tgattttttg
      661 atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt acttgctaat
      721 aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga tctatatgac
      781 cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg
      841 gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga
      901 tattcacgtt atctcgccaa taatcatgtg ctacgacaaa aaatgacaga gatggggttc
      961 cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta ccctaaggac
     1021 tggcgatttg attttaatca tttttatcag tatttaaagc aaaatggatt tgtgatctat
     1081 cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg
     1141 gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa
     1201 aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca
     1261 cgcttttaga aatgcttttc tagaaaaagg aatccaactg actgacaaag aaatccgcta
     1321 accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc cttctgtcaa
     1381 aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg aggaattgta
     1441 tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct tgaaacccga
     1501 cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt ccacgaatgg
     1561 atttaatgct cctatgatga caatcgtagc agaaaccgca aaaaaagaaa ggtattcacc
     1621 agacttcatg gcaacttttg aagacgtcga agggtatggt cgaccgtttc cttatatgat
     1681 ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga
     1741 tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag gtgtaattga
     1801 aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga tggggaaaaa
     1861 gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ccggcgctga tatttgtgta
     1921 aacaatttaa aagagttaac agaatacatt ttgtacgctg acaagaatcc agaatgactt
     1981 ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc aagtaataaa aacagccaag
     2041 caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
     2101 gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc acagtcccct
     2161 tatttgcaaa caaacagtgt ttaagagaca acctctccac taaacggtgt tcattcagct
     2221 gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct cttttgtttc
     2281 tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc ttatctggtg
     2341 catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca taaactagag
     2401 ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca tagtatttgt
     2461 tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg tatttttccc
     2521 cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc cagctggtat
     2581 cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt tccaacaggc
     2641 tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca aatcgaacaa
     2701 cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga
     2761 ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca gggtttttct
     2821 cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct aataagacga
     2881 tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca aatggaatat
     2941 ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa gacagctttc aactgtttaa
     3001 aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg taagtacgtt
     3061 tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg tctgacttct
     3121 taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca cctttatcct
     3181 cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg tcattgtaga
     3241 aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt ttattgaatt
     3301 ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttta tcgccttctt
     3361 ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt tcagctactt
     3421 catggaattg gacgtataaa cgaagcaatt cattgattgg ttctgcttta acagcttctt
     3481 ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc cagtcaccta
     3541 aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg atcgaattac
     3601 cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta ggagaagaca
     3661 tgtcgattgg tactgttcct tgattcccga tgttgctatc cccataatgt tctttttctt
     3721 tcactacggt ttgcaaaact tttttgctga taagttcttt attcatgaaa aagttcaagt
     3781 aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct gctaaatctg
     3841 cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa
     3901 catctccgtg ttcagctgat tttggatttt ctagtaattg ctcgacttgt tccagtgtta
     3961 aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta ttcatcttta
     4021 ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa tctctagcga cggcatactt
     4081 agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc ctaaaaaagg
     4141 accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc tccaagtgcg
     4201 cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc caagaatgat
     4261 acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa ttatatcaga
     4321 aaaaaaaacg aaaggctagt cgtttcataa aaaaaaaatc cagatggcct aaaggagaag
     4381 aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa caaattaatc
     4441 agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt ctaaagaagt
     4501 gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgctt ctactttcgg
     4561 aagcatgctt cctggagcga attgcttttc atcaatgtat tgtttcattt ctgaaacagt
     4621 aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat tgtctactcc
     4681 tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg aagcaaaatc
     4741 tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa caggaatccc
     4801 accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga tgacacgtgc
     4861 ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac gtccagcatc
     4921 ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt cttccgtgta
     4981 gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat ccacaataac
     5041 ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta cttcgcccat
     5101 ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg tatctaaagg
     5161 cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt taccgacttg
     5221 cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt tcactaaata
     5281 ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg tagacaagat
     5341 ggcattgccc cctaaagcga caactacttt tcgtttggcc atttcattcg tcctttcttc
     5401 agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt tccctcaatg
     5461 ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt
     5521 acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg cttggtcaaa
     5581 ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag tgatgccaaa
     5641 gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg
     5701 caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat tgatttggta
     5761 aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg atacccatac
     5821 atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg taatcatcaa
     5881 ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt cttcaggttg
     5941 taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg tgactaataa
     6001 agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac cttctactgt
     6061 tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat gccattcatc
     6121 agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta ccattttttg
     6181 actgaatcca cggaattcga ttccgtcaaa catacgtcct agtactttag ctgtatcttc
     6241 tgtggattct tttttaccta attggatatc atttgcacct agatattcag gatgtgcacc
     6301 tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt tttcaaataa
     6361 aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt tcaagtcttt
     6421 caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt ctttttctgc
     6481 taataagctt cttccttgga atactgattc tttcatatta aacattctcc tttagattgt
     6541 ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagact gccgtcagga
     6601 aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg gaccaccacg
     6661 acctcgtgac aattcacttg aattgatttc taacactttg atgccatagc tacgaagcaa
     6721 ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga ttgccaatgt
     6781 gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat taccagttgg
     6841 gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta agttatctga
     6901 atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca tcttaccgtc
     6961 tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg taaatacggt
     7021 atccaaatgc atcattgcac ggttgttcgg aattttgatt gccaacactt tttcaaatcc
     7081 tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg
     7141 agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc cttcgatatg
     7201 atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc ttggatgatg
     7261 tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag tcatcttatt
     7321 gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat
     7381 tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcatatagac tgctagaacg
     7441 tacttcgata tcttttgtac gaaccccagc catgatttta tctaccattt cttgcgtttc
     7501 cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa tatgagattc
     7561 atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg cttctgccgc
     7621 taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg caaagttgtc
     7681 atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac gatccataat
     7741 atctggtgtc aaattttcca cttcttgtcc aggccgtttc agtaaaactg tctttaattt
     7801 ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc ttctttttga
     7861 ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct cattcaaaat
     7921 gagacattcc tcacattaat attcgataat taaataatta aacgtttttc tatttttaag
     7981 cg
//
LOCUS       DAHEYX010000131         7932 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHEYX010000131 DAHEYX010000000
VERSION     DAHEYX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.132"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            304..492
                     /locus_tag="MB219_001951"
     CDS             304..492
                     /locus_tag="MB219_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCC8427664.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            570..1583
                     /locus_tag="MB219_001952"
     CDS             570..1583
                     /locus_tag="MB219_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8427665.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1650..2684)
                     /locus_tag="MB219_001953"
     CDS             complement(1650..2684)
                     /locus_tag="MB219_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8427666.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2799..5024
                     /locus_tag="MB219_001954"
     CDS             2799..5024
                     /locus_tag="MB219_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCC8427667.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5042..6925
                     /locus_tag="MB219_001955"
     CDS             5042..6925
                     /locus_tag="MB219_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCC8427668.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6906..7880
                     /locus_tag="MB219_001956"
     CDS             6906..7880
                     /locus_tag="MB219_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCC8427669.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2560 a         1452 c         1679 g         2241 t
ORIGIN      
        1 tcattctgtc tcagattttt cgttatttca atttgattaa ctgattgatc aaaaagattt
       61 ttattttcat attctcaaat gattcaacac taaatataca tgtttgacgc ggaaattatt
      121 attcgatttt ggcggcatca ggggggatga cgacgtagca gacccattat tttactcaag
      181 aaaatgtccg tgcctattat ttagaatacg atagtgaacg tgctgggagt tttgttcttt
      241 tagagaaagt ctctgaagat aaattagttg tcttagattt tgactgtaac gaaaaatggc
      301 gaaatggaag agcaccagca agtgattgac cggatctacg aagcgacacg atatctgcct
      361 ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt caacagaaga agaaaatgtt
      421 ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag cattgcagaa gaagtttggg
      481 gaaaatcatt aaatcatcta ggagagcttg ataggaaata acactatggt ataatcagaa
      541 tgcttaatac taactactga gggagcctta tgaaaaagaa aattcttaaa tttatcggat
      601 ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt tcttgccttc cagctttccc
      661 ccagacctgg agcattcttg atcaaccata tgttttcaaa tactgttcaa atcactgata
      721 aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa aacagatttg acgtatgagt
      781 ccaagtataa agagaatact tatgacatct actatccaaa aaacagcaag gggcctgttc
      841 ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg agataaatcg ggtgtcaaag
      901 aatttgcgac aaaattagtt tcagacgcca acatcgcagt ggttgcgatg aattatgaat
      961 tagcgcctga ttcagaatac cctaatcaag ttcttcaagt gaatgaactg atcaaagatt
     1021 tattagctga agaaaaagac gacaaattgc tagacatgga acaattattt tttggtggag
     1081 atagtgcagg ttcacaaatc gcattgcaat atgcagctat acaaacgaat gatgcgtatg
     1141 ctaaacaaat gaaaatcaaa cagctacttc caaaagatag tctgaaagga accatttctt
     1201 attgcggacc tgttgatttg aagctaacag cacagcagca ttcggatgat cgatttatga
     1261 agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac aaaagattgg aaaacgagtg
     1321 agcaattgaa agaagctagt gtggctgacc atgtttctaa ggaatttccg ccaacgtata
     1381 taactgacgg aaatgcatac tcattccagg agcaaggtca agcattagaa aatcggttga
     1441 cagaattaaa ggtacccgtg caatccttgt tttatacaga tacaaaaaaa gagattaccc
     1501 atgaatacca atttgattac acgctaaaag aatcgcaaaa ttgttaccag cagacattgg
     1561 attttgtgaa taagtataag taagaggttg tgaaaaagcc gtttcgtttt gagtatgaag
     1621 aaacgaagcg gcttttcaca acctctcttt caataccttt ttgttcttgg gataaaactt
     1681 tccctaacaa tcaatcgtgc aggataagtc aaaatgattg gttcgtcccg tataccgtca
     1741 attcgctcca atgccattcg aacagctgct tgcccaattg atttcgcatt gatttgaaca
     1801 ctggtcaagc caggggagag gtttgcgatt gtctcgatat tatcaaagct tgctacaaca
     1861 atctcttctc caatttggat atttttttgt ttcaatgccc gatagacacc aattgccatt
     1921 ggatcgctcc cgacaagaat ggcagtagga agaggggatt cattttctaa tagattagtc
     1981 gcaagtacct caccttgttt ttctgtccag ccacctagat gataagacat atatttttct
     2041 aagtgatgat cgatcataaa atgctgatag gcacgcatcc tcttttcatt tatagaaatc
     2101 tgcttcccac cgaattcatc catatcgact ttatatcccc cgatataggc tattctttta
     2161 tgaccatttt ctaagaacaa ttccagtacg gttttagtca tccgttcaag atctgcatac
     2221 accatatcga cctcttgctg gatgtcaggg ttatccacga cgactatttg catgttttgt
     2281 tttaacaagg cggtaatcga ttctttcgta acagtcccaa caacgatcac cgcacctaac
     2341 tgatctagat cttcccattg tttaggatta tcagatagat tatagattcg attcatccct
     2401 aaatgcagac gtcgagcttc ttcttccatc cctactcgca aatgtttgta atagatatcc
     2461 tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa tcgaaggcat tctctttttg
     2521 atatatttac gagtactgat gtcataatcc aacgcagcag ctgcctctac tatccgttgc
     2581 cgagtagtct gagccacatg gatcgtctga tcgttattca acacacggga aactgtagca
     2641 ggagagacat ttgcctttcg tgcgacatct ctaattccaa ccatttttac acactcctca
     2701 aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa cagtatacag gaattataaa
     2761 aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat ggatattacg catagaaaaa
     2821 cagataaaaa tttcatctta tttgatgaaa agagccgagt cttccattta cgaaatacat
     2881 ttctttctta tctgataaag atagaagaaa gcaatgtatt ggcgcatatt tattttggca
     2941 aaccagtcaa gcagtacaaa gacaataaaa attatccccg aagagatcgt ggattttcag
     3001 gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga tacactaccg caagaatact
     3061 cttctcatgg cagcatggac tttcgaacgc ccgcatcgat cattcagcgc aagaatggat
     3121 cagacttact ggatctgaga tatgacagcc attatattac agatgggaag ccagagatcg
     3181 aaggcttgcc tcaaacttat gtgatggaca agtcagaggc acaaacgttg gtgatttcac
     3241 ttaaggatag agaaacagcg atttatttcg atttgttcta taccatattt actgatagag
     3301 cagtgatcac acgatctgta aagataagaa atgaaaccgg agaaacaatc aaactagaaa
     3361 aagcggctag tttccagcta gactttgccc atactaggcg atttgatgaa gtcattgctt
     3421 tgccaggagc acatgtcaat gaaagacaga tttcacggca atctgtttta tctggaacaa
     3481 aagtatttga aagccgtcga ggaacgagca gtcaccatat gaataacttt attgctttag
     3541 ttcatcatca tacgacagaa aatactggag aagcgatcgg acttcaattt gtttattccg
     3601 gcaaccattc tttcgaactt gaaaaagatc agatcaatca attgagagtc gtcggtggga
     3661 tcaacagcca tcgtttttcg tgggaattga atgcaggcca gtcttttcaa acacctgaaa
     3721 tgatcctttc ttactcaagt cagggattga acaaaatgag tcagatccac catgagttgc
     3781 tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga acggccgatt ctggtcaata
     3841 actgggaagc cacttacttt gacttcaata gtgaaaaaat caaggcgatc attgatgaag
     3901 caaaggagct aggcatcgaa atgttcgtat tagatgatgg ctggtttgga aaaagagatg
     3961 cggacaattc ttcattagga gactggttcg agtatgaagg caagttgaca aatggattac
     4021 gtgaaatagc agattatgct catagcaaag gattgaaatt tggtttatgg tttgaacctg
     4081 aaatgatctc ggttgattct gaactgtatc gaacacatcc tgattttctt atgcaggagc
     4141 caggtagaat gccttctgct tccagatcac aacatgtact ggattttaca aggttagatg
     4201 ttcggcagac gattgagaaa caaatgagaa agattttaga tacgattcct cttgactaca
     4261 tcaaatggga tatgaaccgt tcgctttcag atgtctattc gattaccctt gacccacaga
     4321 gacaaggaga agtagcgcat cgttatatgc tcgggcttta tgaactactt gaacatttga
     4381 caactgatta tccggaaatc ttatgggaag gctgttctgg tggaggtgga cggtttgatg
     4441 ccgggtttat ttattatatg ccgcagtcat ggacaagtga taacacggat gcagtggagc
     4501 gtatgaaaat ccaatatgga acaagccttg cttatccgat ttcgtccatc acagctcatg
     4561 tatcagctgt accaaatcat cagaccgaca gatctacttc gttgaaaaca agaggagaaa
     4621 cagctatgag cgctgttttt ggttatgaac tggatttgac aaagctgtct cctgaagaaa
     4681 aaaagcaagt aaaagaccaa attatatcct atcaaacgat tcgtccggtg atccagtatg
     4741 gccattatta tcgtttggcg agtccttttg aagaaaatat tgccgcatgg atgtttgtgt
     4801 ctcctaaaca agatgaagct attgtgtttt tagggaggat actagcttca gctcaaccag
     4861 cgttccatga agtatatctg atggggttag atgatgaggc actttatcag gaacagacct
     4921 cgaagcggat attttcgggg gccgaattga tgacagttgg actttacttc cccgattttc
     4981 aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt atgagagaga aggaaaaaag
     5041 tatgaaagca aatataaaat ggttagatga tccacaagtt tttagagtga accaatcaaa
     5101 tgcacatagc gatcatgttt gttatgcaac aagggaagaa gtagaaacca catcctccct
     5161 taaacagtct ttaaatggat catggcgttt ttgttattcg ccaaatcctt cggtacgacc
     5221 aaaaacattt tataaaaaag attttgatgc ctgttctttt gatgaaatca ctgttcctag
     5281 tcatattgaa ttagcaaatt atgacaagat ccattacatc aatacgatgt atccgtggga
     5341 gggacatgta tttcgccgtc cccctttcac gacaagccct gagaaagagg cggaagggat
     5401 cttttcagct gctaaagaca atccggttgg ttcgtatttg acgacttttc gattagaaga
     5461 aggtctaaag ggaaaacaaa tctcgattca ttttgctggt gtagaacaag caatgtttat
     5521 ttggttgaat ggtcatttta tcgggtatgc cgaagattcg ttcactccgt ctgaatttga
     5581 tttaacacct tatttggaag aagaaaatat tctagctgtg gaggtttaca aaagaagtac
     5641 agctgcattc ttggaagatc aggacttttt ccgatttttt gggatcttca gagatgtctt
     5701 cttagttgct cgaggacaaa accatttaga agatctttgg atcaaacctc gcttagatga
     5761 caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt gagcaagtag ccaaaaacag
     5821 tcagctgaat gtagcgatac tagatcctga tgggaaggaa atctatcaac agcaatatcc
     5881 gttaaaagag caaatgacga ttcgcatcga tggatttgaa aatgtggcac tgtgggggca
     5941 ttttcagccc aatttatata agcttgaatt ggagacgata gacgaagaaa aaagagtaac
     6001 ggagtatatt gactattcct tcggttttcg taaaatcgaa atcaaagata aaatcatcta
     6061 cttcaatgga aaacggatga ttatcaacgg ggtaaatcga catgagtggc atccgaaaaa
     6121 aggacgcgcc atcactatag aagatatgga aaaagatttg gctatcataa aaaatgcaaa
     6181 gatcaacggc gtacggacat ctcattaccc caaccagatc acttggtatt atttatgcga
     6241 ccaagcagga atccatgtga tggcggaaac gaacctcgaa tcccatggct catggcaaaa
     6301 aatgggcgca tttgagcctt cttacaacgt accaggtgca gtacctcagt ggcaggcggc
     6361 tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa aaccatccaa gtattctttt
     6421 ttggtctctt ggaaatgaat cttatgcgga agaaaacatt cgacaaatga acgattactt
     6481 caaaaaagta gaccccgatc gacttgtcca ttatgaaggg aattttccaa atccagcatt
     6541 cgaagattcg atttcagatg taaaaagctg catgtatgcg ccaccagaag agatcattga
     6601 ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt gaatatatgc atgacatggg
     6661 gaattcgtta ggtggaatgg attcttacat gacgttgctt gatcgttatg aaatgtacca
     6721 aggaggctat atatgggatt ttaaagatca ggcgatccaa gtgactgatc caatcacagg
     6781 cagagaagtg atgcgctatg gcggtgactt tgacgataaa ccaagtgact atgaattctc
     6841 agggaatggg atcgtttttg cagatggaca agaaaaaccc gccatgcagg aggtaagata
     6901 ttattatgaa aaatacagta aataaaagtc atatggatac ggaaaaagtt gcaatcgtct
     6961 tcggcgactg tacattaggt gtcaaatcgg ggaatacgca ttatattttt tcttatacaa
     7021 gaggcggact ggaatcgctc aataaaaacg gaaaagaatg gctataccgg gaaacacttc
     7081 cagttttttg gcgagcaacg accgataatg acagaggtag cggatttgga tttaaatccg
     7141 gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga aatagaagta ctggtggaca
     7201 ataaaaagat cgatttgccg attgctccag taaataatca atattcaagt aatgaatacg
     7261 ctgaaacggt tgaaatcatt tttacactgg aaatcaacac agtaccaaaa acccaagtga
     7321 caatttccta ccaaatcgct ggagagggta aaatgcttgt caatatgcat tattatggac
     7381 aaaaaggatt accggaatta cccgcattgg gtttaaggtt cattatgcca acgaaagcaa
     7441 aattttttga gtatgaaggc ctatcagggg aaacatatcc ggatcggatg gctggtggtg
     7501 tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc ataccttgtt ccccaagaat
     7561 gcggcatgca tatggaaacg aaatgggtag aactaacaag aaatcaaacg ttggataata
     7621 cggataaaga tcaagaagat ttttcgttgc gatttgaaca gcaggatacg ccatttgctt
     7681 tttccgcttt accattcaaa gcagaagaac tggaacaggc aacacatata gaagaactac
     7741 cgccagaaag acgaacggtt cttacgattt ttgcaaaagt aagaggagtc gggggtatca
     7801 atagctgggg agcggatgtt gagtcacctt accgaatcag cagcgaagag gaccatgaaa
     7861 tcggctttat tattcagtag caatctttag attattattt gaaaaacgga gtttacaaga
     7921 caaagctgct tt
//
LOCUS       DAHEYX010000132         7899 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHEYX010000132 DAHEYX010000000
VERSION     DAHEYX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.133"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..197
                     /locus_tag="MB219_001957"
     CDS             <1..197
                     /locus_tag="MB219_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427670.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="MB219_001958"
     CDS             314..811
                     /locus_tag="MB219_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCC8427671.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="MB219_001959"
     CDS             898..1290
                     /locus_tag="MB219_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCC8427672.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="MB219_001960"
     CDS             1274..3721
                     /locus_tag="MB219_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCC8427673.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="MB219_001961"
     CDS             3724..5907
                     /locus_tag="MB219_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCC8427674.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="MB219_001962"
     CDS             5904..6905
                     /locus_tag="MB219_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCC8427675.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="MB219_001963"
     CDS             6920..7834
                     /locus_tag="MB219_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCC8427676.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         2463 a         1522 c         1687 g         2227 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggaga
//
LOCUS       DAHEYX010000133         7806 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHEYX010000133 DAHEYX010000000
VERSION     DAHEYX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.134"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            203..2530
                     /locus_tag="MB219_001964"
     CDS             203..2530
                     /locus_tag="MB219_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCC8427677.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2656..2985
                     /locus_tag="MB219_001965"
     CDS             2656..2985
                     /locus_tag="MB219_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCC8427678.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            3025..4029
                     /locus_tag="MB219_001966"
     CDS             3025..4029
                     /locus_tag="MB219_001966"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCC8427679.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            4026..5455
                     /locus_tag="MB219_001967"
                     /pseudo
     CDS             4026..5455
                     /locus_tag="MB219_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5661..6833
                     /locus_tag="MB219_001968"
     CDS             5661..6833
                     /locus_tag="MB219_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCC8427680.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6995..7450)
                     /locus_tag="MB219_001969"
     CDS             complement(6995..7450)
                     /locus_tag="MB219_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427681.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
BASE COUNT         2663 a         1455 c         1537 g         2151 t
ORIGIN      
        1 tatctttttt tttcaataat cgtaaaaaaa gagtattttt tatttaaaaa taaaatcttt
       61 gtattaaagg acaagactaa aataacctta gaataaaaag agaaaaaaag ccccaaactt
      121 tctatacatc aataaaataa taaaaattaa atttagaata acgtgtaaag gattatttga
      181 tgatttttta ttgagaggag ggatgaaaat gattaagaaa actcaaaata aaatccaacg
      241 tatatgtcaa aaacattttg tttctagact caaagctgta tctaaaagaa aatacttaca
      301 tcccattatg atttcgatca tcttgatttt tactttctta gggagcttat caagtattta
      361 tgtaaacgtt tatacagaaa cgtcaaattt tgctaaccaa acgacagagg agacaaatag
      421 tccaccctat tctgaaatac agtccataga aagcaccaat cagtctagca acaacgagac
      481 aacctcaacc acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc
      541 aaaagcagca ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt
      601 tgcggccact gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc
      661 caaagatgga gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca
      721 aggcgtaacc aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat
      781 tggctccaca gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc
      841 taccgctgga aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac
      901 accctcaaca tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat
      961 acagttagca aatgatattc cattcacggg gtactcgatc aaaaatgggc agatcgttga
     1021 tggtaaggga aatgtttcaa attttccaac ctatcaagca gcaacggttc aggtcaatgc
     1081 gaatcggaca ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta
     1141 cgggactaca ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt
     1201 aacgggctat tcttttgcac aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga
     1261 tacggtgacc tatacaggca aacaaacacc aatcactatt aagtatcaag actattttgg
     1321 acagatgatt gctccttcta aaataatgaa tcttacttac ggaagtgctg cacaagacct
     1381 gacgactaat acgcctacta tcaacggata tacctttaca gctgtttcag cgacagaaac
     1441 aaagaaccaa tcagcaatga gtgttttagc tagtttagat acaaatggaa atgtaatcgt
     1501 aaaggacgca aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac
     1561 agtatcaatc aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt
     1621 taaatatgcc tttaatgata aaacagaatc aacatcactt ttaaacaatg atctcagtat
     1681 tgatcaaatc acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt
     1741 aactgcgcct actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc
     1801 taaactagct gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac
     1861 gattaagcaa atcagcggtg cggtagagct cagtggcagt aagacatatg atggaaaagc
     1921 aggtgtcccg tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt
     1981 acaagtggca cttagcagta tggattacac agcagatgca caatcagcaa aaaatatggt
     2041 taatgctggc agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc
     2101 aaatcccaag ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa
     2161 agctaacgca gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc
     2221 gatttcggtt ggaaatctcc acgttaccgg tgttgtctta ggcgaatcat tggattatac
     2281 cctaacagtt aacagccgga cagatattgg atcacaaata gtaacgatta gtttaggcga
     2341 aggaagtgta aacaataatt atacgattat cgcaacagat accgctaagt tgacgatcaa
     2401 tccgactctc ttgcttcctt cgacaggtgg gataggtcta ctgccctttg tctttttagg
     2461 actgatcttt attttttctg gcttctttta ttttatccat cgtaagaaaa aagcaggtga
     2521 acagcgatga aaaaaacaaa aaatgtacgt gtggatatat ttgctgttct cttttttatc
     2581 ctactggtct tttttatagg ttctccgact gtgtcagcta gagaatcacc tattacattt
     2641 actttgcata atcgaatgtt cgaaagcgat aagatgccaa gtatcagtca aaacgacgga
     2701 ataaatatcc cggataactt tcaaggagta aaagggttga acggcgttac tttcgctata
     2761 tatgatgtct cggatgaatt ttacaagctg cggtcagaag gaagtagtgt ggaagacgca
     2821 caaagaaaac tagcacaaaa aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa
     2881 acagtagacg aggaagaggg cattgcttct tttagtgcat cggacaaaga tgagcaaggt
     2941 cgtgacgctg tttaccgatt tgcggaaata aagacttcag attaagtaaa agaaaaatca
     3001 gcaccatttg tcgttgtgct tcctgtgaca gattcatcta atcataaatt gacgaccatc
     3061 cacttgtatc caaagagcga acaaaaaata cctgctgcac tcacattaac aaaaacagta
     3121 gagaataagc agacagattt tgcagatggg gataaagttc cttatctcat caccgtaaca
     3181 attcctgaga acatcaacga gataggaacc tatacgatca aagatacagc tgatcctcag
     3241 ttatggctag aaccagaaac gatcgatttt ttgattggtg gagacaagat tcatacattt
     3301 cacacacaaa aaacaaaaca tggttttgtc ttatcagatt ctggaaaaga tcttagttcg
     3361 tttgctggga aaaaattaac gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa
     3421 acgacatttg ataacgatgt acgtttgacg actgacaatc aaacagtaga aacacatctt
     3481 gccatccaaa caggaggcaa acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta
     3541 gcagatgctc aatttctagt caaaaaaaat gaccaatact tagcgcaaga aaacgggata
     3601 aatcattggg tagccaaaga tgattcaaga gcaacgattt ttacttccag taaagatgga
     3661 acattttctg tccatggatt gtcttttgga agttacgaac tagtggagat taaaccgcct
     3721 aaaggatata tattgagtca atcaaccatt ctctttgaag ttgaaaaagg cagttatgat
     3781 ccgcttcatc ctataccatt ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat
     3841 ggaaaaacgc cagttcagat gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc
     3901 tttccaaaaa caagcgaaga gatgcttgga ggtttctcaa ttttagggtt gttgctagtc
     3961 ctttcaacag ggacagcttg gttttataaa aaacaacaaa aaggaaacaa taggagggaa
     4021 ataaaatgaa aaacatcaaa acaaaaatat ggacgttttt aggaactgct atcatgctgc
     4081 ttcctttcgt tctcggccta ggaactgcag aggtttctag gccgtatcac ctacaccaga
     4141 aaatgttacg gttaatttgc ataaattgaa attcacttca gcaccagaaa atcaaataaa
     4201 caacggaacg gaattgacat ttccgaatag cgaaccgttg aacggcgttg aattcaacgt
     4261 atatgacatc actgctacgt actatccaag taaagacaca gcagttccag cagacgctac
     4321 acccttcgca agtgtaacga cttctggtga aggattagct aacctgactc ttccaggtaa
     4381 atctgatggg aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac
     4441 gtcgccaaat atcgtgttaa gcttaccctt ttataacccg aattcatcag accctgacaa
     4501 acctttagac acgattcacc tatatgctaa aaatgtcatc aaagaaagca atatcaaaat
     4561 ccagaaagct gggaactttt caggggtaga taagttagca ggggcgcaat tccagatcaa
     4621 aaattcagag aatcaattcg tgaccggttt tgatgaaaac actggtgtcg ctctctatga
     4681 tcctgctgag tcagcgttga cattcacaac tgatactgaa ggatccatca gtgtcaatgg
     4741 attaatagac ggcaattata ctttagttga aacaaaagca ccagatggtt atcaattaac
     4801 caatccacaa aaagaaacta cttttatagt aaaaaacgga aaattgcaac aaacaaccgg
     4861 tatcatccat ggcgcagatg aagccaatct tgggaatcca aacggtgtag tcaatcggga
     4921 aaatacaact acggataaca cgattactgt agaaaatgtc caaaactacg gtgatttcca
     4981 cttcatcaaa caagatgtga acacgcaaga aaaattagcc gatgcagaat tttctattct
     5041 aagagcaaaa gatccaactg ttaaattaat gaaaaaaata gcaaatcttg cctcaggaga
     5101 atacccatat gcttggtcag atgaagtcac aggttctgac tgggaagaag tgaaattgat
     5161 ttctgattca gaagggaaat tttcaatcga agggctgaaa caaggggcat actgccttca
     5221 agaaacaaaa gcaccagaag gctacgcatt acctaagaat gaagctgcgt acactgaatt
     5281 tacagtagat gatgatccaa caacttctga tgaaacaacg atcgataacc aacctaaagg
     5341 aatcttgcct tcaactggtg gtatggggat catcgtattc atcttggtag gtactgcttt
     5401 agttggagga gcagtgatct acttcaaaaa acgccatcaa gaaacagaag cttaaactat
     5461 tggaaacaaa aatcatacga taaataaacg atagtgattc aaatggggag aaaggttgtg
     5521 acaagtggtc ggtctcttgg tattcgctca acaaacggca aaataggagg aactattttg
     5581 ccgtttgttg agcgaatact caggtcaagg cgctgatttc acaacctctt catatacata
     5641 ttagaaaata ggtggacatg atgaataaaa gcagaataag aatactggac atcttcatgg
     5701 caatcatttt agtcgttggg atcgggatct tttcttatcc ttttgtagaa gattcactga
     5761 atgattttct tgcccagcaa atgatcatcc attatcaaaa gcaagcatcg aataagaatt
     5821 cagaggaaat aaaaaaacag caggaaaaaa tgacaaagaa aaaccaacag ttggcagagc
     5881 aaaacgtatc accagggata ggcagtttca accaagcagt tgatgcaaaa gcattaaaag
     5941 atttacctag taatgccttt tttatggaac atatgcttgg agtcatcgaa atcccaaaaa
     6001 tcaatgtcag cttacctata ttcgatcaga caacggaaat atttttgcaa aaaggaacgt
     6061 cattattgga agaaagttct tatccgactg gcgggaaaaa cacccatgcc gttctttctg
     6121 ggcatcgtgg actcccagaa gcaaaattat ttacagattt gcctaagttg aaaaaaggag
     6181 accagttttt catccagatc aatggaaaga cattggcgta tcaagtagaa aaaatccaag
     6241 tggtcttacc tgatgaagta gattctttgg gtatccaaaa aggcagagat ctcgtgactt
     6301 tgcttacctg tactccttat atggtcaata cacatcgact gcttgtgacg gggcatcgta
     6361 ttccttatca agcaaaagag gcaaaaaaag cgatccaagg aattgatcaa tggaaaaaat
     6421 ggaaattttt tatttggttt atcggtatac tacttggatc aatcggctta gtttggctac
     6481 tcatcgctta tttagattct ttggccatcg ctaagcgaaa ctatccttta tctttttatg
     6541 taaagaacac aaacggacga ccaatcgaag gaatggtctt ttctgtaaaa acgctcaacg
     6601 gcaaacacta tattactaga gaaaaagttc catttgtgaa agcttctgat gaatacggac
     6661 tagtgagatt ttctgattta aaaggcagga actatcgctt gcaacatgag gagctcctgc
     6721 ttaaaatcca tgtaaaacat aaacattcga aacagttttc aatgaaattg aaaaaaggaa
     6781 gatataaact gcgaaaagaa aaagaggtgt actatttgat tgagaaagaa tgaatgaaga
     6841 tgtccaaaaa agcagaggga acagactaaa tgaaaaaaga gagaagctga cgaaatggac
     6901 gtgtaaactg ctatttttgg tttactatct ggaacgccag cttctatttt tttatgtttc
     6961 agaagaattt tattattata gtataacttt tgaattagtt tccataacat ttggagcaag
     7021 gggttaaacc acgagcttga gcttcgctta aagtggcagg ggtatacgta ccatttccac
     7081 atttatgtgt atggtatttt gaaccggtcg gtgtgacata gacagtttgt tcattatttt
     7141 gctcaggagc tgcggcttgt gcctgctgag aggcctgttc agctgctgct tgctgtcgcg
     7201 cctgttctgc ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg
     7261 caatttcttc ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa
     7321 ttgcagcaag acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg
     7381 aagcagttgc atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg
     7441 cttctgtcac ttttttttct agtgcagctt tttccgcttt ttgtttctct tcttcttttt
     7501 tcttttcaat ttgttcttgt tctttcgatg aatctgccgt actttcggag atcgtggtac
     7561 tgctgctgga agcctgagct gaacttgtgc tttgagtagt agacgtagta gcaccacacc
     7621 cactcaacaa acttcctgcg accaaaaaca atgcaactaa ttttttcact aaatttcctc
     7681 cttaagtaaa aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta
     7741 taataattgt gttatgaata gtgattgagg aaacttaatc taatcccgtt ttgatacacg
     7801 ggattt
//
LOCUS       DAHEYX010000134         7672 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHEYX010000134 DAHEYX010000000
VERSION     DAHEYX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.135"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            9..1196
                     /locus_tag="MB219_001970"
     CDS             9..1196
                     /locus_tag="MB219_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCC8427682.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1177..2481
                     /locus_tag="MB219_001971"
     CDS             1177..2481
                     /locus_tag="MB219_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCC8427683.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2507..3520
                     /gene="citC"
                     /locus_tag="MB219_001972"
     CDS             2507..3520
                     /gene="citC"
                     /locus_tag="MB219_001972"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCC8427684.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3524..3817
                     /gene="citD"
                     /locus_tag="MB219_001973"
     CDS             3524..3817
                     /gene="citD"
                     /locus_tag="MB219_001973"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCC8427685.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3822..4727
                     /gene="citE"
                     /locus_tag="MB219_001974"
     CDS             3822..4727
                     /gene="citE"
                     /locus_tag="MB219_001974"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCC8427686.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4720..6279
                     /gene="citF"
                     /locus_tag="MB219_001975"
     CDS             4720..6279
                     /gene="citF"
                     /locus_tag="MB219_001975"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCC8427687.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6276..6833
                     /gene="citX"
                     /locus_tag="MB219_001976"
     CDS             6276..6833
                     /gene="citX"
                     /locus_tag="MB219_001976"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCC8427688.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6826..>7672
                     /gene="citG"
                     /locus_tag="MB219_001977"
     CDS             6826..>7672
                     /gene="citG"
                     /locus_tag="MB219_001977"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCC8427689.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMD"
BASE COUNT         2622 a         1224 c         1598 g         2228 t
ORIGIN      
        1 gtgaaggtat gcttgatgaa gttttagaaa tccataaaaa aaatgtaggt gttttacgga
       61 tggaaacaac agaagccgtg caagatgatc aagatttagc aaaaatgtac accccaggtg
      121 tagcagaact aagtttaatg attgagaaaa atcatgcttt ggcaagagaa ttaacaatta
      181 gtggtaaatt agtggccgtt attacagacg ggtcggcggt attaggtctt ggaaatgtag
      241 gaaaccaagc tggattacca atcgtcgaag ggaaagcgct gctttataaa aatttagcag
      301 gtgtcaatgc gattccatta gccatcgaac aaaaatcagt agacgagatt gtacaaacaa
      361 ttgtcaattt gcaaaatagt tttgcaggga tccatttaga agatattgca gcaccgaagt
      421 gttttgagat tgaagaaaaa ttacaagaaa aactttctat tcctgtgtat catgacgatc
      481 aagaaggaac agcgatagtt gttttagctg gattgatcaa tgctgctaag atccaaagga
      541 aaacattaac tgaacttcga gttttgatca acggcatggg agcatctggg gttgcaacag
      601 ctagactatt aatcgctgct ggtatcaaaa atctaacatt ggtggataaa caagggagat
      661 taaagaagca agatagtaca ttaaataaat atcaaagaga gttgttggat catatacatg
      721 atacaaataa taatgcaagc gttttggaag atgcaatcgt tggacaagat gtttttatcg
      781 gtctttcaat aggaaatgtt ttaacgcctg aaatgattgg aaaaatgaat gataatccga
      841 ttatttttgc tttagccaat ccagtaccag agatcacacc cgatttagca aaagaatctg
      901 gtgcaaagct aatcgctaca ggttcttcga aatatcctaa ccaagtaaac aatattttag
      961 tatttcccgg attgttcaag ggactattac tatcgaaagc gaataaagtc gatctttcat
     1021 tacaacttga agtagccaaa caattagctg ctaaggtcgc agagcctacg gtagaaaaat
     1081 tcatccctaa tgtttttgat gaaggagtcg caaacgtcgt ttcacaggca gttttggatt
     1141 atttacaaaa actaaaagaa aacgaggata atatacatgc tgaaaacatt aaatagaatt
     1201 aaaattggtt ttattccttt atctctttat atcattttgg tactcgcgta tcttgcaatg
     1261 acgataagca atatcgttcc agaaaatgat atgttagttt ctttgggtat tatgactgtt
     1321 tttgcttatt tattagaaga aattggtaaa actattccta ttttaaaaga tttaggtggt
     1381 aaagtattag ttgttacatt tttgcctgca tttctagtct ataaacattg gctacctcaa
     1441 agtactgtaa ctgtggtttc tgattttatg aataataaca atttcttgat gttctttatc
     1501 acactactag ttgtcggcag tattgcttca atgaatcggc aaacattggt taaagcaact
     1561 tcacgtatta tcattatttt aattttatgc gatgtgattg gttctttgat aggaacaggt
     1621 gtgggaatac tccttgggct atctgcttgg aatgcgtatt tctttatcgt tgcgccgatt
     1681 atggctggtg gtgttgggga aggagcgatg ccattatcga ttggttatgc agcaatatta
     1741 tctggtatgg gacaaaatga tatctttggg aagattctac cgtgtgtgat gttgggaagt
     1801 ttagtcgcag ttctactgtc tggtatgctc aaaaaaattg gagagaaata cccacaatta
     1861 aatggtcagg gtaatttgat cgatggggat gatgaacatt tagagaactt gaagggttca
     1921 gctaaaccag tcaatattga aaaaacattg ttagcaggcg tggtagcaat aacgatttat
     1981 tttgttgctg tctatgttga gcacttatta aaatcactcc ttggcttttc tattccttca
     2041 cctgtgatct tattagtctc tcttatgggg gctaaaatgc ttggttttat ccctaaaaat
     2101 atcgaagaag gtggaaattc gttgtatgga ttcactgttc gtgggatcac acctccctta
     2161 ctttttggcg taggggttgc aaaaacagat tggttagagc tgatcgaagt atttaaaaat
     2221 cctgcaacga tagtaacaat cattgtgacg gtagtagcta ttgtcgctac agcttttatc
     2281 gccgcaaaat ttttaaaaat gtacccagtt gatactgcca tcgcagtttc atgttgtagc
     2341 ggacaaggcg gtacaggtgc tttagctatc ttagctgcgg gtgatcgcat ggaattaatg
     2401 ccatttgcac aagttgctgt tcgactaggg ggtgctatga ccgtaacgtt cgctatcttt
     2461 ttaatgggtc tgctatcata agaacataaa gaaaggacgt gaccatgtgt ttgatcaaac
     2521 agatatccaa cgattatttt taaaaaataa ctatatcaaa gaccaatggg aaaatctttt
     2581 gcttgattca ggaatacaaa agaagcaaat cgaagatttt caacatttag atcaaacatt
     2641 gggtatatac cataaagaaa agttagtagg aacagtatct tatcaaaaca acgtgattaa
     2701 atatatcgcc gtgtctgaaa aatataaaga tggtggacat acatttaatg tactagtctc
     2761 tgcaataatt caggcaatga gtcaacaaga gatttttcat tatattgttt tcacgaaacc
     2821 gcgctatcaa aaaagtttta actttttagg ttttgaaaca atcgtatcta ccccaaaaat
     2881 agctgtatta gaaatgggag atagttctat aaaacagtac ctagagagga ttcctaaagt
     2941 gaatggtgaa aaaaaagcag caattgtcat gaatgcgaac ccatttacag aagggcatta
     3001 ttatttagta aaacaagctg ccaataacaa cgattgggta tatgtatttg tactcgaaaa
     3061 agaacaaaat cttttttcga cgaaggaccg actcaaatta gttcaacaag gattacaaaa
     3121 actgaaaaat gtcattgttc tatcaggtgg cgagtatatt attagccctg caacgtttcc
     3181 cacctatttt ttacgagaag atgatgaaat agcaaaggag caaatggtcg tagatgctac
     3241 tttattcaaa gaaagaattg ccacttattt agatatctct gctagatatg tgggagaaga
     3301 accactttcc ccaatgactc gttcatataa cgacatactg gaaacgattt taccccctga
     3361 aataaaagtt catgtccttg caaggaagaa aacggaacaa caccaagtta tttctgcttc
     3421 tcaagttcgt aaagcgtatc ttgctgggca attggaaaaa ataaaatata tggttccaga
     3481 aacaacctat caatacttaa aaaataaaag ggagcgttaa gtaatggaaa taaataaatc
     3541 agcgattgct ggaacattag agtcaagtga tgtccaaatc atgatttcac cgaatacaga
     3601 aggaatagaa atcgatttag acagtcaagt gattgagcaa tttggaaaac aaatcaaaaa
     3661 agttatctat gacacactag cagtttatca gattaaaaac gtaaaactca aggtagtaga
     3721 taaaggggca ttagattgcg ttattgttgc acgattaaaa acagcgatcc atcgttcctt
     3781 aggtatccaa caccaagaaa caccgtggga ggtttaatta gatgatgaaa gaacgtttga
     3841 gaagaacaat gatgtttgtt ccaggaaata atccagcgat gatcaaagat gcaggtattt
     3901 atggtgctga ctcgattatg ttcgacctgg aagatgcagt gtctatggca gaaaaagaca
     3961 gcgcaagaat gcttgtggct gaggcattga aaacagtgga ttatggtgac acagaattaa
     4021 ttgttcgaat aaatgacctg cactcagaat ttggacgtgc agacattttt gcaatggtta
     4081 aagccggcgc gcatgtatta cgtttaccta aaacagaaac ctctgaagat ataacagaag
     4141 tagaaaaagt gattatagaa gcagaaaact atcataatgt tccgataggg cgaacattga
     4201 tgatggcagc cattgaaggt gcttctggtg tattgaacgc acctgcgatc gccaaagcat
     4261 ctgatcgatt gattggaatc gccattgggg cagaagacta tgtgacaaat atgaaaacca
     4321 aacgatatcc agatggaaac gaattgtttt ttgctcgcag tatgatcgtt catgctgcac
     4381 gtgcagcagg catttccgca tttgacactg tttactctga tgtggatgat atggaaggat
     4441 ttttaaacga agtacgttta atccatcaat taggttttga tggaaaatct tgtatcaatc
     4501 cacggcaaat tccagtagtc aatactgttt atcagccgtc aatcaaagaa atcaaacgat
     4561 cactagacat catctcggca atcgaagaag caaaagaaaa aggttctggc gtaatttctt
     4621 taaatggaaa aatgatcgat aaaccaattg tagaacgtgc acaacggaca ttgatgttag
     4681 caaaagcatc acgattgatt gatgaggagg gaaatatcca tgcataataa acaaagacgt
     4741 agcattccat caagtgtttt aacaaagtat aaaaaagaat tccaaagtgt ttcattatcg
     4801 ccacaaacga ttcaacgttg tgcaccaaaa gtaacgactg taactaatca gacaaataaa
     4861 ttgatgcact cgatcaaaga agctatagaa aaatcgggct taaaagatgg aatgacgatt
     4921 tcatttcatc atcatttgag agaaggtgat tttgttttta accaagtgat ggccgtgatc
     4981 cgtgaaatgg gcatcaaaga attgacctta gcaccaagtt ctttaacgaa cactatgaat
     5041 gagatggtta ttgaatgcat caaggacggt acagtaactc atatcacaag cagtgggatg
     5101 cgcggcagct taggtgaatt tatttctcat ggaggaatgg acaatcctgt gattttacgt
     5161 tctcatggta atcgtccacg tgcaattgaa agtggtgaat tgaaaatcga tgttgcattt
     5221 ctaggagtac caacatctga tgattatgga aatgctaatg cgactgttgg aaaatccgtt
     5281 tttggttcat tagggtatgc aaaagtagat gcacaatatg cagataaagt cattcttcta
     5341 actgatcatt tagttcctta ccctaatacg cccattagta ttccgcaaac acaagtcgac
     5401 tatgtcgtgg aagtagaagc aattggtgat ccgaataaaa tcggtgctgg tgctacacgg
     5461 tttactaaaa acccaaaaga attaaaaatt gctgaaatgg tcaaagatgt cattgttcac
     5521 tcaccttatt tcaaagatgg cttttctttt caaacaggaa cgggtggtgc agctttagca
     5581 gtaacgcgtt acttagctag tgaaatggaa gaaaaacaga tcaaagcttc ttttgcatta
     5641 ggtggcatca ctaaaccaac ggttgattta ttggaaaaag gtcttgttga aaaagtgtta
     5701 gatgtccaag actttgacaa aggagcagct gaaggaatgg cgcggcttga gaaccaacag
     5761 gagatcgatg cttcctttta tgctgatcca agtaataaag gagcggttgt taaccaatta
     5821 gatgtcgcta ttttatctgc attagaaatt gacacaaact tcaatgtaaa tgttatgact
     5881 ggttcagatg gtgtgctaag aggtgcgatt ggtggacatc aagatgcagc aacggctaaa
     5941 cttactatca tctcagcgcc gttagtacgt ggccgaatac ctacagtagt aaatgatgtt
     6001 actacattga tcacaccagg gaaaagtatc gatgtattag tcacagaagt aggggttgct
     6061 atcaatcccc aacgtaaaga tctaatagct atttttgaaa gaattccaca aattcctgtt
     6121 tttacaatag aagaattaca gcaaaaagca gaaaaaatag taggcatccc ggaacctttg
     6181 caattcacag atcggatagt tgcctatgta gaataccgag atggttcaat tttagatgtc
     6241 gttcgtcagg tgaaagaatt tgaagaagag agatcatgat gtttgatact ggagaaattc
     6301 aagaaatcga ggatattttg atccataaag aacaacgtgc taacttgcaa agcaacctgc
     6361 tttccaagta ttcaaatagt tcattgatgg tctttacatt gaatattcct gggccgatca
     6421 aaaataatcc aatgattgag caggtatttc gtcagggaca gcagtcaatt cagcaaatgt
     6481 ttttaaaaaa aaaggtggaa atgacgtaca caaaagaaat caatttgcca agtggattgg
     6541 actggtacgt aatagtaaca gaagcacctt tgaatctcaa aaaatggttg gtcacgttag
     6601 aagagcaaga cgcattaggt agactttttg atttagatgt cttgttcaaa aaaggagagc
     6661 agataacaat tgtcagtcgc caagctctta atcatgggga acgtccttgt tttatttgtg
     6721 ggaaatcagc aaaggtatgt gcacgtaatc gtacacatac tgtgaaagaa atgcaaaaga
     6781 aaatcgaaca aatagtagcc gattccttaa aaggagcatt gaaacatgca tgataatatt
     6841 caaaaaatag cacaagaagc acaattagcg atgctttatg aagttacatg tatcaataaa
     6901 ccaggattgg ttgatccagt agaccccggg tctcatcaag atatggacat ttttactttt
     6961 ctacaaagta gtgtagtttt gactccctat tttgaagaat ttatccaaac aggattggac
     7021 cgacaacatc aaccaattga agaaaccttt atggcgattc gacagattgg tttagaggct
     7081 gaaactgcga tgttctcagt gacaaatggt ataaatacac ataaaggtgc tatcttttca
     7141 ttggggattt ttcttgctat ttgtggccgg ttaactattt ggaaagttcc ttgtaaaaaa
     7201 agtttctttc aaaaagaaat tcaaaaaatg acgaaaaatc tacttaacga ttttgggaag
     7261 attgatcaaa caaaaccaaa agcatggact tggggagaat atctatttgt taaccatgga
     7321 ttgacaggga tacgagggga agccaaaaga ggttatcctt gtgtttttga ccgaggattg
     7381 ccttattatc aagcatatca agggtcgcaa caagataaga tgatagacac tttacttttt
     7441 ctatcaatgg aagtagaaga taccaatttg atcaaacgaa gtaataatag acatgttttt
     7501 gaagagtatc aattacttat tcgtccttat tttgaattag ggggtgtaaa aaccatacaa
     7561 ggtaaaaaat atttggatta cctaaatcag caatttaaaa agaaaaattg gagtattggt
     7621 ggaagtgcag acttattgat ccttacaatc tttttagata aaatcatgga ca
//
LOCUS       DAHEYX010000135         7639 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHEYX010000135 DAHEYX010000000
VERSION     DAHEYX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.136"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            191..889
                     /locus_tag="MB219_001978"
     CDS             191..889
                     /locus_tag="MB219_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCC8427690.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            1067..1408
                     /locus_tag="MB219_001979"
     CDS             1067..1408
                     /locus_tag="MB219_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427691.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1401..2072
                     /locus_tag="MB219_001980"
     CDS             1401..2072
                     /locus_tag="MB219_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCC8427692.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2078..2764
                     /locus_tag="MB219_001981"
     CDS             2078..2764
                     /locus_tag="MB219_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427693.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2771..3622
                     /locus_tag="MB219_001982"
     CDS             2771..3622
                     /locus_tag="MB219_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCC8427694.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            3634..3903
                     /locus_tag="MB219_001983"
     CDS             3634..3903
                     /locus_tag="MB219_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427695.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3908..4072
                     /locus_tag="MB219_001984"
     CDS             3908..4072
                     /locus_tag="MB219_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427696.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            4065..4385
                     /locus_tag="MB219_001985"
     CDS             4065..4385
                     /locus_tag="MB219_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427697.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            4382..4771
                     /locus_tag="MB219_001986"
     CDS             4382..4771
                     /locus_tag="MB219_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427698.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            4768..5361
                     /locus_tag="MB219_001987"
     CDS             4768..5361
                     /locus_tag="MB219_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCC8427699.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            5358..5735
                     /locus_tag="MB219_001988"
     CDS             5358..5735
                     /locus_tag="MB219_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427700.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            5732..5935
                     /locus_tag="MB219_001989"
     CDS             5732..5935
                     /locus_tag="MB219_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCC8427701.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            5932..6141
                     /locus_tag="MB219_001990"
     CDS             5932..6141
                     /locus_tag="MB219_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427702.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            6138..6431
                     /locus_tag="MB219_001991"
     CDS             6138..6431
                     /locus_tag="MB219_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427703.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            6461..6901
                     /locus_tag="MB219_001992"
     CDS             6461..6901
                     /locus_tag="MB219_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCC8427704.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            6976..7443
                     /locus_tag="MB219_001993"
     CDS             6976..7443
                     /locus_tag="MB219_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCC8427705.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIIIQFASSLELVAFK"
BASE COUNT         2840 a         1166 c         1631 g         2002 t
ORIGIN      
        1 tattttttct atctgtatag gagatataaa aagcaaatgt tgaaaatact agagacgtaa
       61 taggcaaaat aaaatcttta atggttttaa caatgttatt ccagtcaaat tgtttaataa
      121 agtcaataag actcaattta taccaccacc attttttatc tacattatat caaagaggag
      181 agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagcat
      241 acaagtggca gaaagctttg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact
      301 aaaaaaagat gtgtccaatt ttggacagat gttttctgaa ggtaatgagc cagattccta
      361 cggaagaaat cgacgagttt tcttcattag cagagatgga tttttcttgt tagctatggg
      421 atttacagga aagaaagcta tttacttcaa acaaaaatac attgaagcat tcaacgaaat
      481 ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagacatga tgatctacca
      541 actagaagaa atgaaagatg ttaaaaaaga tgtctccatg cttaaagata ctatgcgaat
      601 tagcggacaa caagagtttg aaattaagca aaaaggaaat acgaaagtta tggaagttct
      661 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
      721 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
      781 cgatgatgct gtttcattta ttgaaatgtg gttaccagaa actgcgattc gcatggaaat
      841 cgatcaactg aacagacaac aaagactttt tggtgatgaa aatgaataga gctgaagcgc
      901 taagaatcgg gacggtaatt gctaatcgct ggtggagaca caataaacca atcatcctaa
      961 gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc
     1021 aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact
     1081 ttccactcta gatcaatatt tgactgatcc tagttggggc aaatcgaata tcaaggaaac
     1141 aaataatcga aaaatcagac gaaatctttt gacggatgaa gaactagcat gtgatcaaga
     1201 cgatttggga aattttgtga gtatttggga tcatgtctat cttatccatt tatcgaggaa
     1261 gtccaaaaaa cctgaatata tctatgtcat cgaagatggc ttgattgatg cgctagaaga
     1321 gtatgacaga gataacttga ttgatatctc ttattacgga tcaggtaaga aatacattgc
     1381 tgaaatggag gcagaatttg atgagtgaaa acaaagggac aacgactttt gaaaaacttt
     1441 ttagtcgcaa gttaaataaa atactcaaga aaaaaggaaa ttttgattat ttatcttggg
     1501 ctcacgcatg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
     1561 ataaacacta cagagttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
     1621 cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca
     1681 cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg
     1741 caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac
     1801 tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacgaaga
     1861 aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga
     1921 ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata
     1981 acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag
     2041 cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc
     2101 atattcagcg gttctgaaaa agttcaaagg aaaagacata ggtgcaacca tcaatgagga
     2161 agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc gagttattga
     2221 aattcgtttt attgatccta gacgtttcac agtacagcaa cgaaacttca tctatgcact
     2281 cataggcgat attttcatcg atacaggcat gccaacggac ttctggaagg aattctttta
     2341 cttccgcttt gaaggtgtca cagggcgcaa aataagcctc aaagatgaat cgaatacgac
     2401 tgtgagtgat gctaacgtct tagcaaatat catcttagat ttcatctttg aacatcatat
     2461 tcctttcaaa gaaggctatg agattttacc agcgaatcaa gaatattact tctacaaatg
     2521 catcacaaaa agagtctgtt gcatctgtgg caaaacagga gctgatatcg atcactttga
     2581 caaagcgcta ggaagacgaa agcgcaaaga agttgatcat tcagagtaca catttgcagc
     2641 actctgcaga atccatcaca cggagaaaca caagataggt gtgatcaatt tcaaaaataa
     2701 atatcaaatc aaagggatca agttaaacca ggaaacaatt aagaaactta gaataggagg
     2761 ataaaaaata ttggctgaca acaaacgata ctactattta aaactaaaag agaacttttt
     2821 cgatagcgac gagatggttc ttttagaaag tatgccagat ggatacattt attctaatat
     2881 tcttctcaaa ctttatttaa gaagtctgaa acatgaaggc aaactaatgt ttaatgacag
     2941 gattccattc aattctacaa tgcttgcgac tattacaaga cactctgtgg gagttgtaga
     3001 aaaagcagta caaatatttc gtgatttaca acttattgag gtattagata acggagcaat
     3061 ttatatgtct gatatacaaa gttttattgg gaaatcttca actgaagctg ataggaagag
     3121 aaaatacaga aaagaaattg aggaagcaaa acagaattta ataacaagtg gacaaatgtc
     3181 ggacaaatgt ccggacaaaa ctacaccaga gttagagata gagttagaga aagatataga
     3241 gttagagaaa gatatagaga atgtaacgcc ttcgaaaaaa tcgaaggcta agcccgtccg
     3301 tcataaatac ggagagtata aaaatgttct tttgtcagat gagcagatgg agaaactcaa
     3361 aatagaattc cctaatgact accaagagcg aatagaacgg ctatctgagt attgtgaatc
     3421 atctggtaag acttataaaa actatttggc aactattcga agctgggcaa gaaaagaaaa
     3481 aagtgaatct aagaacgcaa gcagtggata caagcgcaca ggaagacgag agaagcttcc
     3541 tgagtgggca atcgaccaag aagcctatct caagaaaaaa gcgctagaac gagctaatag
     3601 acaatcaaaa gcaccattct aagaggtggg aaattgaaaa tcgattatct agaactaatt
     3661 aatgaaatag caaagtataa aactggtgag gaaatagaaa tcctgagaga cgtatatgat
     3721 caactcgaag aagctggaat cgaaggaatt aagaatgatc gttcgagttg gagtaagctc
     3781 agatactatt tcgcacttta tatcgataca acacaattaa gaaatttagc atataccaaa
     3841 ttactatttg ttgattgtgt taaaggcttg caaaaacatc ttaatgaact tgagcaggtg
     3901 taatcagatg gacctaaaga catttacagc acagattgaa ctaatgcatc aagaagcttt
     3961 aagacaaagt gtatcgtacg aagacaagtg gctcaacacg ttccacggtg gacgtgagag
     4021 cgcacttgat caagtactca aattattgaa aggggaacgt cgggatggat aagaaagcgg
     4081 cttggcgaaa attaatgttg ctgattcaag atgagaactg gcaagaagat gaagcagtgg
     4141 ttgctgaagt tcagcgtcta gaaaagattg ctaacggacg tatacgaaaa aagcctgata
     4201 agcggaaact tcgcaaagga aaaatcatcg ttgttttgta tgaaggtaac attttgatgc
     4261 aaggaacggc aagagagttg tctgcagaaa ctgaatatac gagcggaact attcggacgt
     4321 acgcgtggcg gaatcacgtt gataaaaaag gacacgaata taagtatttg gagggcagca
     4381 aatgataccg aagtttagag cgtggtacac accatttaaa ggtaaaacaa ttggacaaga
     4441 aatgaaatat gggcaagcag gaaggttgat cactcatgct gaaatggctc cagataaata
     4501 tgtgcttatg caatccacag gactgaaaga caagaacggt gtggggatat ttgaagggga
     4561 tgtagttctt gtcaatgtaa gtaatggttt tgatcattta gttaatgaaa aaacggttgt
     4621 acaagaatcc gagtttcatt ctggattagt ttgtaaatca ttaattagtg gaatggaata
     4681 tagagtattc aaacacaaaa agatggatta tgaatatgaa gtcatcggaa atatttacga
     4741 gaattcagag ttattggagg aacagcgatg aataaacagg aaacaatcaa aaagttagaa
     4801 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tatgaatgca
     4861 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac acgttatgcc gaagtttttt
     4921 gacaaatggg caaaacaagt cctggaaaaa cgtgacaagt tttatgccat ttctcttatt
     4981 gcacgtgcag gttggggata tggtgttgat tttgaactta gcgagaacgg attatcatca
     5041 gaaaacaaag agctgttgta ctggcttgtt gataaatgca gcgacaatta tcctaataaa
     5101 aagaaagcaa tagaagcttt gttatacggc tacgaggtcg agaaagaacc gttgtatcac
     5161 gtgaagctaa aaataccagg cgtaacatac taccttatcc aaactttttt aatgccagga
     5221 gatactcata tgtgtttctc agcggctaca gaacgttatg gggctaaatg gaaaaatact
     5281 tttacggaat cagaaataaa agcaattgat gaaagatact ggccatttgc tgtgccagtg
     5341 gaagaggagg cagaaggatg aaaaaattgc accaactcat atctgagaaa gaaagcgaac
     5401 tccaaaattt agaagatagt ttgggattgg ggtttccaat tgttgagcaa gctaaaatga
     5461 ctcaaatcag tcatctacga cttgaattgg aagatttaag acaaatagaa aaatcaattc
     5521 aactcaacga caaccagcaa atcgtgtttg agtggttgaa actaactgcg ccaacaggaa
     5581 agccaatgca agttgtattt tggatgatga ataatgcagc atgggggcat ttagacgaat
     5641 taagggatcc tttgatggaa ttaacagaca aagagcaatt cgaagtccta gcagcattcg
     5701 ctcaatgggg attagaacag gaggaagcgg aatgaacaac agacatcgca gaataacgaa
     5761 actaagaaaa caggaactga atgtactaaa ggcaaagttc gaaaaagaat acggagtttc
     5821 tgcagaagaa gcatataaag tggcaagtca gtgtgttgct gatgcgagta atgctattcg
     5881 taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggatatag aatgaaggtt
     5941 tatgtagtaa ggttcgggaa taaattttat agaagtgatg aacgctctat aggcaataat
     6001 gtgttattcc ttgtagattt tcttcaaaac gcaagatggt ttgataatct cgaagaagct
     6061 aaccaagttt cacgacgact tggtggatta acgcaagtat atgaactggt cactatcgat
     6121 catgaggggg tagaaagatg aaactaaaag acggatttta cgctagtagt catggtatcg
     6181 gcggtttaat gctaggtatg ccgacaaaga accctaaaac acgtaagaaa ccaaaattca
     6241 aagtcggtga catggttcgc tgcgaagcag aggagttcat ctatccgttt cgtggatatg
     6301 tagagcatgt ttataatcac tcagcgatca ttcgcattga aaacacgatg gaatgtgaca
     6361 agcggttagc gaaaagcaaa gagaatttag cagtggtgag attggtggat atggaagtta
     6421 taaaatctta attttgcttt tttacaacta tcctacaaca atgagcgtta ttccacaact
     6481 ggaaggagaa gaggcaattg tgaacatttt agagaatata gatattaagc aaacaaggaa
     6541 aaatgctaga cgattactaa aaagatacag aaatttagaa cgtttggtcg gaccagtgaa
     6601 aatagacttt tccgtgatga ctgttaccaa aaatttgaaa ttcacaattg acagtcaaaa
     6661 cgaagaaatc attgaagcga taagtactcg agattcggtt atcgaagcat taacacggct
     6721 aagtagaatc catttccagg tactttatta tagttattgt tttcctaata agatgtcaat
     6781 gtatcaaata ggagaaaaat taggatattc cgatagaact atcgagagaa tgaaggcagt
     6841 agctttggtt gaatttgctg aggcgtataa atcaggagaa ctcatttcac gtacaaaata
     6901 aaaaaagtcg gattcctccg actatgaata atatttccga cataagtatt atatcataat
     6961 tgggggaatc agaggatggt acttttcgat gtaaagaaat atgagacacc aagcgcgaaa
     7021 gatgttgata tggaacgcac aaaacataat gtcgctgtgt ttctttcagc atatctatca
     7081 gctagatgta gagtaggtca acctcgagaa ccaaaagtga cagcatctta ttccttggtt
     7141 ccaccttcta cagctgatca tatatttgaa gcagaaagaa tgctgatcga taaagaagaa
     7201 gcacaggaag aatttgagta tctgcataaa ttgtttgtcc gaggctattc agcgatacag
     7261 catccgcata agcctgatgt gactgaaagg cgcaagaaga tattctatga tcgttatatc
     7321 aatggcatgt cgatttatgt aactgcccaa agaaataata cgagcgagga atctgtaaaa
     7381 gcagaatcaa acaaaatcat cattcaattt gcttcttcct tagaactggt tgctttcaag
     7441 tagccagttt ttacactttt tatacccttt tattaccaat ttggtttcca ttttatacct
     7501 tttttatacc aatcacttac ctattcaacg ttgtattatg atagtgtcga aagattagga
     7561 aacaggatcg acaaaataaa atgtaaggga ggaaatctcc ctcatcgttt aattaagctt
     7621 cgataggcag caacggaaa
//
LOCUS       DAHEYX010000136         7637 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHEYX010000136 DAHEYX010000000
VERSION     DAHEYX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.137"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            371..3178
                     /locus_tag="MB219_001994"
     CDS             371..3178
                     /locus_tag="MB219_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCC8427706.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLGTFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3369..3860
                     /locus_tag="MB219_001995"
     CDS             3369..3860
                     /locus_tag="MB219_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8427707.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3963..4952
                     /gene="manX"
                     /locus_tag="MB219_001996"
     CDS             3963..4952
                     /gene="manX"
                     /locus_tag="MB219_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCC8427708.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4985..5788
                     /locus_tag="MB219_001997"
     CDS             4985..5788
                     /locus_tag="MB219_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCC8427709.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5809..6723
                     /locus_tag="MB219_001998"
     CDS             5809..6723
                     /locus_tag="MB219_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCC8427710.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6806..7180
                     /locus_tag="MB219_001999"
     CDS             6806..7180
                     /locus_tag="MB219_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCC8427711.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7319..7564
                     /locus_tag="MB219_002000"
     CDS             7319..7564
                     /locus_tag="MB219_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCC8427712.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         2475 a         1263 c         1683 g         2216 t
ORIGIN      
        1 ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt
       61 gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg
      121 tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa
      181 aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat
      241 caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt
      301 ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga
      361 ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca
      421 aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa
      481 agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg
      541 ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta
      601 caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt
      661 tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc
      721 gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa
      781 gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga
      841 aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag
      901 tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat
      961 tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga
     1021 aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac
     1081 aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc
     1141 ttctctatta ggaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga
     1201 aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa
     1261 ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt
     1321 gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt
     1381 tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat
     1441 tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat
     1501 attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc
     1561 ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc
     1621 ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca acatccatct
     1681 gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt
     1741 tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt
     1801 accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt
     1861 gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt
     1921 tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt
     1981 tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg
     2041 ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc
     2101 aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga
     2161 catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga
     2221 agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa
     2281 ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc
     2341 tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca
     2401 tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga
     2461 agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac
     2521 agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt
     2581 agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga
     2641 atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc catttattcc
     2701 tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc
     2761 agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt
     2821 tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg
     2881 gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa
     2941 aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt
     3001 gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc
     3061 agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta
     3121 ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat
     3181 acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta
     3241 aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta
     3301 ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag
     3361 ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg
     3421 caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg
     3481 ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa
     3541 atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa
     3601 aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac
     3661 agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca
     3721 attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag
     3781 tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag
     3841 cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta
     3901 atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa
     3961 atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg
     4021 gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag
     4081 gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg
     4141 aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat
     4201 tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg
     4261 aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg
     4321 aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga
     4381 aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag
     4441 acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg
     4501 cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat
     4561 caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa
     4621 atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca
     4681 aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag
     4741 acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta
     4801 aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg
     4861 tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga
     4921 aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct
     4981 tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga
     5041 aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt
     5101 agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt
     5161 aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc
     5221 agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat
     5281 tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt
     5341 tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat
     5401 gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt
     5461 gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac
     5521 agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa
     5581 catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat
     5641 ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt
     5701 gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc
     5761 tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa
     5821 aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa
     5881 ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca
     5941 atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa
     6001 ttctttaata cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa
     6061 gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt
     6121 atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg
     6181 ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc
     6241 ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac
     6301 aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa
     6361 ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc
     6421 aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat
     6481 aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta
     6541 gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt
     6601 ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca
     6661 atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta
     6721 taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc
     6781 ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt
     6841 aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga taggtgataa
     6901 tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga
     6961 agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat
     7021 tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg
     7081 tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt
     7141 agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa
     7201 gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt
     7261 atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat
     7321 gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac
     7381 cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag
     7441 tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc
     7501 atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc
     7561 ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg
     7621 gctttattta tttaaaa
//
LOCUS       DAHEYX010000137         7615 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHEYX010000137 DAHEYX010000000
VERSION     DAHEYX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.138"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..692)
                     /locus_tag="MB219_002001"
     CDS             complement(<1..692)
                     /locus_tag="MB219_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCC8427713.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1129..1698)
                     /locus_tag="MB219_002002"
     CDS             complement(1129..1698)
                     /locus_tag="MB219_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCC8427714.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1772..2095)
                     /locus_tag="MB219_002003"
     CDS             complement(1772..2095)
                     /locus_tag="MB219_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427715.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2249..3061)
                     /locus_tag="MB219_002004"
     CDS             complement(2249..3061)
                     /locus_tag="MB219_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427716.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3415..4263
                     /locus_tag="MB219_002005"
     CDS             3415..4263
                     /locus_tag="MB219_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCC8427717.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4408..5349
                     /locus_tag="MB219_002006"
     CDS             4408..5349
                     /locus_tag="MB219_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCC8427718.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5339..7510
                     /locus_tag="MB219_002007"
                     /pseudo
     CDS             5339..7510
                     /locus_tag="MB219_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2270 a         1512 c         1364 g         2469 t
ORIGIN      
        1 tatcgttgtg gaatatttaa ataccaacgc ttgtcatgaa aatcttgtgc tccaccctta
       61 gtatttcctt ctggatcgtt tgtcgcacgc atcatgacgt atactttctt attagggaaa
      121 tcacgcatat tgaaagatac atgatagcca acatttccta aagtattata agcttgattt
      181 acgtctggtc tataaatccc atcagctctt actctagcta gttcttttcc agtattgtag
      241 tccataatga aaatatactc gtatttataa ttagcaatgt gccatccagc tacatgcaag
      301 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc
      361 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
      421 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacacct
      481 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
      541 aaatgcgatt tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat
      601 ttcattgctg aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct
      661 ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta
      721 ctctacatct tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt
      781 cagctagaac gagacaagca ggaatacctt tagaagatat caaaggtttt ttaggtcata
      841 aagatgtttc cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa
      901 tgaatcaact agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt
      961 tttaagctca ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat
     1021 cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg
     1081 gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg
     1141 tagaaaaatt ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc
     1201 tatgatttca gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt
     1261 cccagtttcc atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt
     1321 aataaaacct agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt
     1381 aatgagcgag tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac
     1441 ggaagctgtt ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt
     1501 cactgtttga cgaaggacat ctagttcttg caagagcgtt tcgtattgtt cttgggtcag
     1561 catcacgcca gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc
     1621 ttgagcaaat acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc
     1681 taatttcttt aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga
     1741 ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga
     1801 tatcggtaag aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt
     1861 cgttatggtc tagtggaaaa aaatgatcga tgatcactag atctgcatac cgaggcggct
     1921 tatttcgagt acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta
     1981 ccccagcaag tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt
     2041 caggagggtg gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc
     2101 actctactgc ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat
     2161 ctttggaatt gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc
     2221 ggtcatggat accaattacg taacgaagtc attcttggta aagtactttt gtcagctttg
     2281 atttgctttc gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca
     2341 caatcgtcaa tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa
     2401 tctggttaca gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct
     2461 gctcctctct aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta
     2521 aaatctcgat ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa
     2581 aggactgctc tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag caaaacagca
     2641 gatatcttac ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt
     2701 ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt
     2761 aaggttcatt caatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa
     2821 caaacaagag ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat
     2881 gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg
     2941 ttttaacatc gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca
     3001 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
     3061 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgggt tcactcctaa
     3121 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
     3181 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
     3241 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
     3301 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
     3361 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
     3421 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
     3481 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
     3541 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
     3601 tttgaatttc gtccgggacc gatctttacc acgatcttgt tagcagatga gatcaatcgt
     3661 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
     3721 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
     3781 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
     3841 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
     3901 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
     3961 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
     4021 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
     4081 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
     4141 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
     4201 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
     4261 tagattgagg tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct
     4321 ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt
     4381 gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa
     4441 gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt
     4501 tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg
     4561 cttcccagca atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa
     4621 gaagtccaat tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt
     4681 tttacagcct atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt
     4741 cctgtgactg ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg
     4801 gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct
     4861 tatcgtccag gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag
     4921 tggttatacc gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg
     4981 ggttcgccca gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg
     5041 agaaaagaaa agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac
     5101 cccggtcata cctattttgc ttctttggaa gctggcgatg agaaaacttt tttcgcacac
     5161 ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc
     5221 tcagaagcta ttgaaacatt gtcaaaagat cgaacggttt atcttgttta tttcgtagaa
     5281 aaacaactca tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt
     5341 gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg
     5401 tgctccgata tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat
     5461 tggtgtccta tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc
     5521 tttgccagta atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt
     5581 atggatctta cagttttttt cgtcagttac agaggaattc ttccatctat cccaaggtaa
     5641 actttattat ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt
     5701 tattacattg accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta
     5761 cttcatgatg cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt
     5821 aactgttttg ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcgag
     5881 aaacactcga ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc
     5941 tatcttgcct ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat
     6001 ccgagaatat cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg
     6061 aagtgcaaga acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa
     6121 tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa
     6181 aaatcattac accggttctg gatgaataga agatcaggac gcacccatcc aaccaatcca
     6241 agaattccct gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac
     6301 attgacccta aaaaaacaag ctttcttacc ttatccttat ggaaatacta ccttgactga
     6361 tttttcatta gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc
     6421 tgtcccgaat gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag
     6481 agaagaatta aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct
     6541 tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa
     6601 tgaagcaact ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa
     6661 tttccgctat tccaaaacgg ataccgggca tactccaaaa gaacacgatt atgtggatta
     6721 tttcctcttt gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct
     6781 tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac
     6841 aggcacagat aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg
     6901 gccagaggta tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc
     6961 caatccaatg ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga
     7021 acaaccaaat gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat
     7081 tccatcagaa gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt
     7141 gtgtttatcc gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag
     7201 ttatcttatc tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct
     7261 taaccctttg gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct
     7321 gcgaatagaa caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga
     7381 agcagaaata tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga
     7441 aataacgcat ataataagaa aaacggtata caaaaaaatt ctgacaaaag ggagccgctt
     7501 caaaaaataa gataaaattt ctggagaatc tacttctcga atgccgagaa ccagctgatt
     7561 gagtatcgat tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttt
//
LOCUS       DAHEYX010000138         7321 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHEYX010000138 DAHEYX010000000
VERSION     DAHEYX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.139"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            117..3644
                     /locus_tag="MB219_002008"
     CDS             117..3644
                     /locus_tag="MB219_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCC8427719.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3641..>7321
                     /gene="addA"
                     /locus_tag="MB219_002009"
     CDS             3641..>7321
                     /gene="addA"
                     /locus_tag="MB219_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCC8427720.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFL"
BASE COUNT         2522 a         1265 c         1538 g         1996 t
ORIGIN      
        1 aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag aacaaatgtt
       61 cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg aaaagcatgt
      121 ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg atcgacgaag
      181 catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt cccaactaca
      241 gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc ggaaaaaaaa
      301 ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg tatttgctgc
      361 aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca atgatcttgc
      421 gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa atcaataaaa
      481 gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga aatattcaac
      541 cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag caacttaaaa
      601 tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa cgtgcactaa
      661 caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat ttcaacaacg
      721 tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa ttactgcagg
      781 caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct tatacagcgg
      841 atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc cgtctttttc
      901 aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca gagcagactc
      961 gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc caaaagaaag
     1021 cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct gaaaatccaa
     1081 ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa gaaaactacc
     1141 gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat atccttcggc
     1201 cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg atggaaaatc
     1261 atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct tatcggctaa
     1321 gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca gaaaccgaac
     1381 ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc actgaaaatg
     1441 tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac tggcagtttg
     1501 tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa gaacagtcaa
     1561 atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa ataaagaaaa
     1621 gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa agtggcgtac
     1681 agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta gaagcggcaa
     1741 gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat gtagtaattt
     1801 atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt ggtctagaaa
     1861 atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat cgtttggaat
     1921 tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat caagtgtttc
     1981 cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta ttaaatcagg
     2041 ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct tttgagcctt
     2101 tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt tatgcacaaa
     2161 gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc acagaatatt
     2221 tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct aattgttatg
     2281 tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta gctttagatg
     2341 aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg aattcgtcat
     2401 ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt cctgtttcga
     2461 tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca atttcacgta
     2521 tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg aaattgaaag
     2581 aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat gaatcattgg
     2641 atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg tccgatgcgc
     2701 agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt cggtttgaga
     2761 tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag acgattcaaa
     2821 aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct cttcagacag
     2881 agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa ttaccgttga
     2941 gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta gcttctgaac
     3001 aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca ttcgatgtca
     3061 cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta gcattgatgg
     3121 atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat tatttacatg
     3181 ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg aaaaaattta
     3241 gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat cattcactgg
     3301 aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat gaataccaaa
     3361 aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta ttcatggcgc
     3421 ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa atcaaactaa
     3481 atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc agaagcatct
     3541 gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa atagataagg
     3601 aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa ttcctttaaa
     3661 accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc aaggagataa
     3721 tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc gaagagtgat
     3781 tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat ttactgaagc
     3841 tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg tcaatagcga
     3901 aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc ccacagcgaa
     3961 tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt acttaattga
     4021 tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga tgaaggagga
     4081 tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat ttttccaatt
     4141 aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg tgttttctct
     4201 ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt taagcgataa
     4261 ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa tccgtccttt
     4321 agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc agttagcaca
     4381 aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc agatcaagaa
     4441 tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt tggaacaact
     4501 aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt ccaatgaagt
     4561 caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa gttattttcc
     4621 tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag tcgaagaaat
     4681 gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg aaaaaggtgt
     4741 gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa aaacgaaaga
     4801 tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag aagtaatggt
     4861 agatgaatat caagacgtga accaattaca agaagccatt ttatactggc taagagaacc
     4921 cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca tctattcttt
     4981 ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa agaaagaagg
     5041 cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc tttcttttac
     5101 gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt atgacgaagc
     5161 agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg agccggaaat
     5221 catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg atgatatact
     5281 ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc aattaattga
     5341 ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag agtacaaaga
     5401 cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga ttttcaaaac
     5461 attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga cagaaattcg
     5521 cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc cgcttgcggc
     5581 tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca ttcgattagc
     5641 taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa acgaagatga
     5701 attagcagct aagctggaac attttggcaa gcaactagag cattggcgag aacttgctcg
     5761 ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat atctagagta
     5821 tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat tggttgatcg
     5881 tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc gtttcattga
     5941 aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag aagataatgc
     6001 tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg tatttttgtt
     6061 agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt ttgaagaaaa
     6121 acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg atacgttacc
     6181 gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa tgcgtaaatt
     6241 gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct ataaaaataa
     6301 agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag tttttgatcc
     6361 tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg gattgatccg
     6421 tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc ttcaacattc
     6481 aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac aaagacagtt
     6541 gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg aaactttatt
     6601 agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt cttcacaaac
     6661 cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag atgatacaca
     6721 agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc aatttcgtta
     6781 tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag tgtcagcagc
     6841 tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga tgccaactac
     6901 agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag acgaaaaagt
     6961 cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac ttggtcaaca
     7021 gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc tgcttcctgc
     7081 tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc acggaatcgt
     7141 cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa cagattttat
     7201 tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc aaggacagct
     7261 aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa atgttttcct
     7321 a
//
LOCUS       DAHEYX010000139         7308 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHEYX010000139 DAHEYX010000000
VERSION     DAHEYX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.140"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..380
                     /locus_tag="MB219_002010"
     CDS             <1..380
                     /locus_tag="MB219_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427721.1"
                     /translation="GIEYSSNLGDVLFQFSQTLARFIPTEVSEKKNEDQKEAMCFSLS
                     QSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYERLGSDTNLSFRY
                     SMNPDDEETDEGIIAKWTKNKIE"
     gene            452..847
                     /locus_tag="MB219_002011"
     CDS             452..847
                     /locus_tag="MB219_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCC8427722.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            831..3284
                     /locus_tag="MB219_002012"
     CDS             831..3284
                     /locus_tag="MB219_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCC8427723.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3281..5308
                     /locus_tag="MB219_002013"
     CDS             3281..5308
                     /locus_tag="MB219_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427724.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5305..6327
                     /locus_tag="MB219_002014"
     CDS             5305..6327
                     /locus_tag="MB219_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCC8427725.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6344..7273
                     /locus_tag="MB219_002015"
     CDS             6344..7273
                     /locus_tag="MB219_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCC8427726.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         2439 a         1287 c         1471 g         2111 t
ORIGIN      
        1 ttggtatcga atacagtagt aacttgggag atgttttatt tcaatttagc caaactcttg
       61 ctcgatttat tccgacagaa gttagtgaga agaaaaatga agaccaaaaa gaagccatgt
      121 gtttctcatt aagtcaaagt gattcagaag atcccagaaa aaaatattgt ttttcagtta
      181 gaagaaatcc tttaaaggga aatggtgagc tgggaaaacg aagtcctttt aatgacaata
      241 aaacgagatt gtatagaccc agcttatatg aacgattagg gtcagatacc aatttaagtt
      301 tcagatattc aatgaatcca gatgatgaag aaactgatga aggtattatt gcaaaatgga
      361 caaaaaataa aattgaatag ttaaagcatt gaaagagcaa tcacctattt tgattgttct
      421 ttttctattt ttacggaaag gacgtgaaga catgaaaaaa ataagaagtt acaccagtat
      481 ttggtcagta gaaaaagtga tttatgcgat taatgattta caactccctt tcccaattac
      541 ctttacgcaa atgacatggt ttatcgtttc tttgtttgtc gtcatactct ttggcagttt
      601 accaccactt tctctgattg acggagcact actcaaatac ttagggattc ccattgttct
      661 cactgggttc atgtgccaaa agacctttga cggaaagaaa ccctatggat ttctaaagtc
      721 tatcattacc tatgctctac gccctaaagt aacctacgct ggcaaacaaa tacgattaaa
      781 aaaagaaaca atgaatgaat cgattacgat tgtaaggagt gaacaactag atggcatatc
      841 cgattaaata tatggaagat aatctcgtat ttaatcatga tggcgaagta ttcgcttatt
      901 atgaattaat tccctataat tacagttttt taagttccga tgaaaaaatt caagttcatg
      961 ataatttctg tcaattgatt gcccaaaatc gtgatggcaa gattcatgct ctgcaactag
     1021 caaccgaatc aagtattcga gccacacagg aacaatcgaa aaaagaaatc actggacgat
     1081 taaaagagat agcttacgaa aaaatagatg aacagaccga agcgttaatt tctatgattg
     1141 gtgacaatca agtagactat cgctttttta ttggttttaa actctacctc aatgaacagg
     1201 aagtatccgt aaaaaatgtt gggaaagaaa ttcaaaaagc atttgcggaa ttctttcatg
     1261 gtgtgaatca caaattaatg ggcgattttg tttctatgcc tatggcggaa atcgaacgct
     1321 tttctaagat ggaacagttg ttagaaagta aattatccag acgctttcag attcgtaaat
     1381 tggagaaaga tgattttggt tatattattg aacatcttta cgggcaaaca ggtgtccctt
     1441 ttgatgacta cgactaccat ttaccgaaac gatacgaaga taaaaaagtg ttggtaaaga
     1501 aatacgatat tctcaaaccc actcgctgtt taatcgaaga aaatcaacgc tatttacgga
     1561 ttgaacagga agataccact acttatgtgg cgtattttac aattaatagt attgttggtg
     1621 aattggattt ccctaatagt gaaatttttt attaccagca acagcagttt actttccccg
     1681 ttgataccag tatgaatgtt gaaattgtcg ccaataaaaa agccttatct accgttcgca
     1741 ataaaaagaa agaactgaaa gatttagata gccacgcatg ggaaagcgac aacgaaacca
     1801 gtactaatgt ggtggacgct ttagaatctg tcgatgaatt agaaagtaat ttagaccaaa
     1861 ccaaagaatc tatgtacaaa ctaagctatg tggtacgggt atcagcaaag aatttggacg
     1921 agctgaaaca acgttgtgat gaagtaaaag atttttatga tgatctgtct atcaaattag
     1981 tacgaccttt tggcgatatg ctaggcttgc ataatgaatt tattccctca agcaagcgat
     2041 atatcaatga ctatattcaa tatgtcacgt cggatttttt ggctggtctt ggtttcggtg
     2101 ctacacaaat gattggtgaa aattctggta tttacgtcgg ttacaatctc gatacaggca
     2161 gaaatattta tctccaacct agccttgcaa gtcaaggaat taaaggctct gtaacaaatg
     2221 cattagcgtc cgcttttatt ggttcattag gtggcggaaa atcattctct aacaatatgc
     2281 tggtctatta ttcagtactc tttggcggac aggcaaccat tgttgaccca aaagctgaac
     2341 gtggtgaatg gaaagaaacc ttacctgata ttgcccatga aatcaatatt gttaatctca
     2401 cgagtgagaa cgacaataaa gggttacttg acccttatgt gattttaaaa ctcccgaaag
     2461 attcagaaag ccttgccatt gatattctca cgttcttaac aggtatttcc agccgagata
     2521 gcgagaagtt tcctgtcctg cgtaaagcta ttcggagtgt aactcaaagc aaacaacgag
     2581 ggttgttatt ggtgattgat gaattgcgaa aagaagatac gcccatcagc aatagtattg
     2641 ctgaccatat cgaaagcttt gtcgattatg attttgccca tctgttattc agtgatggca
     2701 gtgtcaccca atccattagc ttggaaaaac aactcaatat tattcaagtg gcagatttgg
     2761 tcttacccga ttccaatagc tcttttgaag aatacactac catggaatta ttaagtgtgg
     2821 caatgctgat tgtcattagt acctttgctt tagactttat ccactctgac cgttccattt
     2881 ttaaaattgt tgaccttgat gaggcttgga gttttttaca agtggcacaa ggaaaagctc
     2941 tatctatgcg attggttcga gcaggacgtg ccatgaatgc aggggtctat ttcgttactc
     3001 aaaacgcgga cgatttacta gatgaaaaga tgaagaataa tattggcttg aaatttgcct
     3061 ttcgttccac tgacctagtg gaaatcaaaa agaccttaga attttttggt gtggacaagg
     3121 aagatgaaaa caatcagaaa cgcttacgtg aattagaaaa tggacaatgt ttagtttcag
     3181 acatctatgg tcgtgtaggg gttatgcagt ttcacccgat ttttgaagag ttgcttcatg
     3241 cctttgatac cagaccgcct gtgcgaaatg aggtggaaga atgacacaaa aacaaaagaa
     3301 atttcttcac tatagttgga taacgctcct ttgtgtgggc gttattttac ttttattagg
     3361 cacgctgggc aatgcagtac aagcgacagg cttagttgat gaaaccattg atacaagcaa
     3421 tgaatactcc aagtatggtt tgaatcaata tcagcttgat tattatgtgg acaatagctg
     3481 gggctggctt ccatggaatt ggagtgatgg cataggacaa tcggtgatgt atggattgta
     3541 tgccattact aattttattt ggacaatttc tctttatcta tcgaacgcca caggttattt
     3601 ggtgaaagaa gcctatgcct tagatttcat tagtcaaaca tcccaagcaa ttggcgaaaa
     3661 catgcagacg attgcaggca ttacaccgaa tggcttttcc agttcagggt tttatgtcgg
     3721 tttcctcctt ctttttattc tcattgttgg aatctatgtt gcctatacag gattggttaa
     3781 gcgagaaacg acgaaagtga tacgagctgt tttaaacttt gtcgtggtgt ttattctatc
     3841 ggcttccttt attgcgtatg cacccgatta tattgcgaaa atcaatgatt tttccaaaga
     3901 tgtcagtacc gccagtctga atattgggac aaaaattgtt cttcctaaca cggatagtca
     3961 agggaaagac agtgtggaca tgatacgtga cagcttattc tctgttcaag taaagcaacc
     4021 atggctattg cttcaatatg gctccactga tgtagaagcg ttaggggaag aacgtgtgga
     4081 aagtttattg tctaccagtc cagatacaaa caatggtgaa gatagagaaa atgtagtgat
     4141 tgaagaaatt gaagatcatg acaataccta tttaacccta ccgaagacga tttccagatt
     4201 aggaacagtc ttctttttgt ttgtattcaa tattggtatc tcgatttttg ttttccttct
     4261 aacaggaatc atgatttttt cgcaagtgct atttattatt ttcgctatgt ttttgccaat
     4321 cagtttccta ttaagtatgt tgccgagttt tgatggcatg ggcaggcatg ccattaccaa
     4381 gttatttaat gtgattatga caagggctgg tattaccttg attattaccg ttgcctttag
     4441 tatttccact atgctttatt cgctgtcagc aagttctccc tttttcatga ttatgttttt
     4501 acaaattgtg acctttgcag ggatttattt taaactgggc gatttaatga gcctattcac
     4561 tttacaaagc aacgactctc aaaacatggg cagacaagtg tttcgcagac ctcgtcaaat
     4621 gatgaatcga caaaccagaa gatttcaacg cagtatgagt aagatttttc gtggaaaagc
     4681 agggtcaacg aagcaaaaga caatgacaac aaagcagaat caaaccaata aagctaacca
     4741 tactcgtcaa aatgaacgga ctacaccaaa gaaagaaaca gcactttcca atgtgaaaaa
     4801 gcaacaaggc ggtatcgggc aacgattagg tgaaaagaca gcccatgtat tggacacgaa
     4861 aaatcgtcta gtcaataaaa gaaagcaggc aaaacaacag atacaggcga caccaaccca
     4921 tgtcaaatat gcccttcatc aaggaaaaga gaatgtcact cataatgtaa cagactttaa
     4981 acaatctatg gcagatacca aagctcaacg caaacaacag cgtatgaacc agcaggagca
     5041 aaaacgaaag aacattgctc aaaagcgttt ggagctggat aaagcaaaag aaaaaagacg
     5101 aaaagaaaac ccatcatctc ctgttcagaa taaccagcag catgttcgca agcgtcagca
     5161 aaccgtcacg cctgttttgt cacaaaacac taaacgtgaa aatcatcagc gaccattgaa
     5221 aaaagagcaa aaacaaaaga caccaccgaa gcaattcaat cagaggaagc taaataaaag
     5281 aacggataag cgaggacaac aaacatgaag ctaaaaataa cagtgatttg tagtgtggtc
     5341 ttctctctct tcttttttct cttattattc ctagtcattt tctttgcaga tgatacggac
     5401 agtggcgaaa acaacaagga ttcttctatt ccacaaggcg gtgtgaccgt ttcaccagaa
     5461 gtgttggcac accgtccgtt gattgaaaaa tatggtaagg aatatgggat tgaagactat
     5521 gtttcttata tcttagccat tatgcaagtg gaatcgggcg gtacagcaga agatgttatg
     5581 caaagttccg aatctttggg tttaccgccc aacagtttga gtaccgaaga gtccatcaaa
     5641 caaggggtta aatatttcag tgaattatta accagtgcag aacaacaagg agtggatata
     5701 gattctgtta tccaatctta taattatggc ggtggctttt taaattatgt gaggagtcat
     5761 gggaaaaaat atacctatga attagccgaa cagttttcta aagagaagtc gggtggtcaa
     5821 aaagcagact atcccaatcc tatagccata cctgtgaatg gtggttggcg atataactat
     5881 ggaaatcagt tttatgttca gttggtttca caatacctaa ccgacacttc cccaacagaa
     5941 tttgatgatg aaaccgtgca agtcattatg gacgaagcct tgaaatacga agggtttccc
     6001 tatgtctttg gaggggcgtc gcctaccacc tcttttgatt gtagtggctt aatacaatgg
     6061 gtgtatgaca aagctggcat ttctctccct cgtgtcgccc aagatcagta tgacgctacg
     6121 caagaaatct ctatggaaga agcccaagca ggcgacctta tcttctttca ttcaacctat
     6181 aatgcaggca catatgtgac tcatgtggcg atatatttag agggaaatcg cttttatcat
     6241 gcaggcgacc caatcggtta tggcgactta agcagtcgtt attggcaaga ccatctgatt
     6301 ggggctagac gtgttattca taactaagaa aggaatgaat caaatgatta taaaaatcga
     6361 gcgaaaagaa aagaaggaga aaccaaagaa atctaaaaaa ccgagcaagc aaagaaaagt
     6421 tccgatgatt aaagttggca cgcataagaa attaaccttt gttttatggg ttttattgat
     6481 tggtagtgtc ggatttggaa tctataaaaa ttttactgcc attgacactc atactgtccg
     6541 tgaaacagaa atcattaaac agcagattgt cgataccaac caagtagaaa gttttgtgaa
     6601 atcctttgcg aaagactact tttcgtggca acaatcccaa gaagccattg ataaacgaaa
     6661 tgaaaaattg acccattatt tgacggaaga attgcaggta ttaaatgaag aaatgattcg
     6721 taaggatatt cctactagtt ctagtgtgaa cgatatacag gtgtggcagg tttctcaagt
     6781 gaatgagaac acctttgagg tactgttttc tgttgaacaa gttattaccg aagacaaaga
     6841 caaggaaact atatcgtcta gctttcatgt ggttgtccat atagatgaat cagataatat
     6901 ggttattatc aaaaatccga caatgagtaa gaaacctcaa aaatcggact accaacccaa
     6961 acagctagaa agtgaccata cagtagatac agaaacgatg gacgaaatca ctagcttttt
     7021 agaaacgttc ttccagctct atcctaccgc cacagaaaaa gaattgactt actatgtaag
     7081 taatcatgtg ttacctatga ttaacaagga atatgttttc gaggaattgg tgaatcctat
     7141 ctttaccaga aaagataacc aagtaatcgt gaatgtcgct gtgaaatatt tagatcaaga
     7201 aacaaaggca acccaaatct cgcaatttga gttgatatta gaaaaacaag ataattggaa
     7261 gattgtaaaa taacaaatat tggtacatga ttacagatac tttgtaat
//
LOCUS       DAHEYX010000140         7104 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHEYX010000140 DAHEYX010000000
VERSION     DAHEYX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.141"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(34..513)
                     /gene="rlmH"
                     /locus_tag="MB219_002016"
     CDS             complement(34..513)
                     /gene="rlmH"
                     /locus_tag="MB219_002016"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCC8427727.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            941..2230
                     /locus_tag="MB219_002017"
     CDS             941..2230
                     /locus_tag="MB219_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCC8427728.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2269..2874)
                     /locus_tag="MB219_002018"
     CDS             complement(2269..2874)
                     /locus_tag="MB219_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCC8427729.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3070..3534)
                     /locus_tag="MB219_002019"
     CDS             complement(3070..3534)
                     /locus_tag="MB219_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCC8427730.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3556..3876)
                     /locus_tag="MB219_002020"
     CDS             complement(3556..3876)
                     /locus_tag="MB219_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCC8427731.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4019..5098)
                     /gene="pepA"
                     /locus_tag="MB219_002021"
     CDS             complement(4019..5098)
                     /gene="pepA"
                     /locus_tag="MB219_002021"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCC8427732.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5298..5648
                     /locus_tag="MB219_002022"
     CDS             5298..5648
                     /locus_tag="MB219_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCC8427733.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            5833..6786
                     /locus_tag="MB219_002023"
     CDS             5833..6786
                     /locus_tag="MB219_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCC8427734.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            6799..>7104
                     /locus_tag="MB219_002024"
     CDS             6799..>7104
                     /locus_tag="MB219_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427735.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     "
BASE COUNT         2099 a         1369 c         1340 g         2296 t
ORIGIN      
        1 atttttatgc tatttttctc ttaaaaaata tcattatttg tggtatggag cccccgcgtt
       61 gatccggaag gcacggtaga tttgctcgac gaggatcagg cgcatcaatt gatgtggata
      121 cgtcattttg ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
      181 taaagaaccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
      241 ttgtttagca aaagattcgc ttgtgggatt tttcccttca attgctaagc catagacgta
      301 ttcttgttct ttgattttcg ctaagatccg ttctccttct ttttctttta cttgtctcat
      361 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
      421 cgcataggca ttcagccgtt tgacatactc attgatacct tggatcaagt atttttcttt
      481 taattttcct actgaaataa ttttgatatt catgttttct ctcctttgat cttcacgctc
      541 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttgactat ttttgttaaa
      601 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagcgttgt
      661 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
      721 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
      781 acacaatatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
      841 aaagtttttc ataaaaagtt caaagtctca aagtagacta aagtcaacaa ataagactat
      901 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
      961 accaaagatg aaaaaaaata aaaataatag cctctggcgt aagttgggtc tggggcttgt
     1021 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
     1081 caacaatagt tctactgcta caagcggcaa tcaaaattct gccggtgaaa cagttgtgga
     1141 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
     1201 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggattatt
     1261 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
     1321 aagcggcgta atctacaaaa aagacgggaa tactgcttat gtagtcacaa acaatcacgt
     1381 agtagatgga cagcaaggtt tggaagtcat gatgaaagac ggtacgaagg taaaagctga
     1441 attagttgga acagatgcct attcagattt agcggtgttg aagatcaatt cagacaaagt
     1501 cgaaacggtt gcctcttttg gggactcaag tgctttgaag gttggtgaac ctgcgattgc
     1561 gattggttcc ccattaggat ctgaatatgc gaattctgtc acttcaggaa tcatttcttc
     1621 cttgaaccga caagtaacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
     1681 ccaaacagat gcagcgatca acccgggaaa ttctggtggt cctttagtga atatcgaagg
     1741 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcga cttctaatgt
     1801 aagcgtagaa ggaatgggat ttgccatccc aagtaacgat gtagtgaata tcatcaacca
     1861 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
     1921 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
     1981 tgtcatcgtg acaaatgtcc aaacagctac accggcagaa aaagctgggt tgaaacaata
     2041 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
     2101 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
     2161 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
     2221 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
     2281 tacttctccg actacggcat cctcaggcaa tactaagatt cctttttttt ctggagcatt
     2341 tggcaaatgc aattcacgag cagaacaaat cattccatag ctggctaccc ctcgcaaagt
     2401 tcctggccag atcatcattc catctggcat catggctcct ggttttgcaa cgacaacttt
     2461 ttgtccagct tcgatatttg gtgcaccgca aacaatttgc aatactgtcc cattgtccac
     2521 ttctgtctca gtaatcgata aatggtcaga gtcaggatgc gggacgcatg tttttacata
     2581 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
     2641 aatcaatcga ttcaatttcg ctaattcttc atctgttaag cttacttggc cgttcccttc
     2701 aattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
     2761 tttgatccga gcaacattcc atttacgttc gacagtgttt tctgttcctt tatcgtcggc
     2821 tatgataacc atcaaagtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
     2881 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgagat
     2941 ttttttcatg ctttatttcc gaagaagctt cttctggaac accatttatt cgtaacaagg
     3001 gactgtgaca agacttttgt cacagtccct ttactgtttg attaaaaagg ttatacaagt
     3061 tccacttgct taattactcg gctgttcttc ttcgtcagaa acgatcaaga catcacaagg
     3121 tgaatgacgt atgacgtagc tggctacact tcctgtcata aagcgttcga ctgcatttag
     3181 tccagactgc ccaaccatga ttagatcgat tgcatatttt tttggcaatt tgactgccat
     3241 ggattcacga acagagccgt atgcaataat tccttctata tttgtgtaat caacagattt
     3301 tccatattct ttacattctt cgatcatttg cttggctgtt tcagtttgct gatcgactag
     3361 attttgatca ataggagcat agcccataaa attataaaat tgctgatcga ttacgtttgc
     3421 tacatatact ttcccattat tacggcgagc cacttctaac gcttttttga aagcagcaag
     3481 tgcttgttca ctaccgtcaa tacctactaa aatgttctta tattcttgat tcatcttgac
     3541 ccctcctaac agatgtcagc cttttattac actttctagg aacgattcga tttcctgttt
     3601 tgttttacga tctttattca ctagacgacc taattctttt ccgtcttgga tcacgacaaa
     3661 acttgggata ccaaaaatgt tccattcagc ggctacatct atgtacttgt cacggtctac
     3721 ttcaataaat tgccaatcag aaaagtcatt ttcgatttca ggcatttgcg gtttgataaa
     3781 acggcagtct ccgcaccagc cggcagtaaa gaaaaataca ctttttccag tttctacgta
     3841 gcctgctaat tcttctaaac tatttgggat aatcattctt tttcctctac tttctgttta
     3901 tgtatttttc aattaaatta tacacctttc tttcgaacta acaaacattg atcctctttt
     3961 tataaaaaaa atctgacaaa aggttgcttt tgccagattt tatagtttca ctatcgcgtt
     4021 atttcccgta tacgatcgtg ttcactgttg atttatctaa acctttcaat gtttgaatca
     4081 acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc gtatggatat
     4141 aacgtcccac tacgccaatc actgtacttg ggattccttc attttgtgta tgagcagcac
     4201 ctgcatctgt accgcctttt gaaacaaagt attgataagg gatgttgttt gtttcagcga
     4261 tatctagcaa gtactcacgc aaacgaggaa gcatgatcaa acctggatct tggatacgca
     4321 tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgtg tcatctgctg
     4381 cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa gctttagaac
     4441 cacgcaagcc tacttcttct tggacattcg cgcctgcaat caatgtatga cctaatgttt
     4501 cattttgtaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg ttgtcccacg
     4561 atttgctgat gatattcttc ccgttggctg ttttgatcgt ttcagcgtaa ggaacgattg
     4621 tgtctcctgg aagaacaccg taactcattg cctcttctct tgattcaaac ccagcatcga
     4681 acaaaatatc tgttacttct actgattttt gtccgcttgt tcctcgtaat aaatgtgggg
     4741 gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt gtaaaacgtt
     4801 gagcagaaac aacgtaaggg ttccatccgc ctagaggtac gactttaaaa agcccgtttt
     4861 cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc attacacgag
     4921 gtgcatccgc ttcttcatgg tggcgcagcc caaaaatgcc gcccaaacca tctaactgta
     4981 actcatctac cagcggttcc atatgacttt ccatataggc gcgaacgtct tgttcaaaac
     5041 cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt tcttccatat
     5101 gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct gcggataaaa
     5161 tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg gtagcggtta
     5221 tttatttcaa agaaaatatg agagtgtggt aaaataaaca caaagagtag caagtgagaa
     5281 gaaggaggta tatccacatg tatgaagaaa gcgaattaag ctattttaaa ggcgggttag
     5341 ccgttgggat cggtctagga ttattgagtg gggtagcttc aacgatttgg tatcatcgca
     5401 aaaaagtgtt atcagcagac caagtgcttg aaaatatcaa aacatctttt ttaaaagaag
     5461 gcgcaatcga aggtgcttgg atctcttttg aaaaagaacc tattcggaaa ttcgccgttc
     5521 ggtcgagtgc ttatcggggt gggctttccc ggatagaaga tggcgaaact gtttattatg
     5581 aatttttagc agatgctcat acaggaaccg tattagacat ttcaagaaaa aagggcgcaa
     5641 tagattaata cgtcaaaaaa atgaaataac gaatacgcac ccactatcta aaagagaggt
     5701 atcctatcct tcttttatag tgggtgattt ttataaaaat agggctattt cctcttggaa
     5761 tagctagtat ttgtagttat tatacggtat tcttgaaata agattagttt tattatataa
     5821 ggagagtgac aaatggaatc acgtgaaaaa attgccgtgc tcattccttg ttataacgaa
     5881 gaggcgacaa tttcaactgt tattgaagat tttaaaaggg aattaccaga agcagatatt
     5941 tatgtctatg ataataattc gaccgatcga acatacgaat tggccgttca aggcggagca
     6001 atcgtaaaga aagaaccacg tcaaggaaaa gggaatgtaa tccgtcaaat gttttttgat
     6061 attgatgccg attattattt gatggtagat ggtgatgata cttatcctgc agaagctgca
     6121 catggtttgc ttgagaaatt acgttctggc gaagcagata tggtcattgg cgatcgtcta
     6181 tcaaatggaa cctattttga tgaaaataag cgtcctttcc atgattttgg gaataatctg
     6241 gttaaaaata caattactta cttatataaa acaaaaattc gcgatgtcat gaccggctat
     6301 cgaggattca atcgaatttt tgtcaaaagc ttccctatta tgagttctgg ttttcaaatc
     6361 gaaacagaat tgacgattca tgctttagat aaaaaattca agttcgttga gattccaatc
     6421 gattatcgcg atcgtcctga aggtagcgaa tcaaaattga atacattctc tgacggattc
     6481 aaagtgatca tgatgatcat caaaatgtgc aaagattata aacctttgct tttctttggg
     6541 atctggacca tcttcttctt tttatttgga ttggtcatgg ggattcctgt gattcgtgaa
     6601 tatatgctga caagctttat tacacgcatc ccttctgcga ttttaagtac tggattgatg
     6661 actttagccc ttctttcatt agtaacaggg ttgattttgg atacggtcgt cacaaatgcc
     6721 aagaaagaat atgaattgaa tttataccat gtctaccacg aatatcaaaa taaagaaatg
     6781 aagtaggagg ccttttttat gcataagaaa aaggagaatc tatttcctac tttttttgca
     6841 actttatttg cgtcaggttt tgcttttttt acttcttatg gtctaggaac tttgaatcca
     6901 acagtaatca ttggtttagc catggttttg ctacttgctt ttaagcaata tggaatagag
     6961 ttgttttcaa taaaaaacaa agttgcagct attgcccgat tgtttttttc tgcagctttt
     7021 gctcttacac tttctattgg cagacagata caatttcagt atgtctcagc aaactataca
     7081 aagacaacga ttgctgatgg aagt
//
LOCUS       DAHEYX010000141         7102 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHEYX010000141 DAHEYX010000000
VERSION     DAHEYX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.142"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MB219_002025"
     CDS             complement(576..1592)
                     /gene="tsaD"
                     /locus_tag="MB219_002025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCC8427736.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MB219_002026"
     CDS             complement(1589..2053)
                     /gene="rimI"
                     /locus_tag="MB219_002026"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCC8427737.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MB219_002027"
     CDS             complement(2060..2602)
                     /gene="rimI"
                     /locus_tag="MB219_002027"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCC8427738.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MB219_002028"
     CDS             complement(2586..3308)
                     /gene="tsaB"
                     /locus_tag="MB219_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCC8427739.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3499..4479)
                     /locus_tag="MB219_002029"
     CDS             complement(3499..4479)
                     /locus_tag="MB219_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8427740.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MB219_002030"
     CDS             complement(4503..5987)
                     /gene="galT"
                     /locus_tag="MB219_002030"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCC8427741.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MB219_002031"
     CDS             complement(5999..6988)
                     /gene="galE"
                     /locus_tag="MB219_002031"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCC8427742.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1933 a         1553 c         1261 g         2355 t
ORIGIN      
        1 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
       61 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      121 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
      181 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
      241 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
      301 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
      361 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
      421 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
      481 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
      541 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
      601 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
      661 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
      721 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
      781 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
      841 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
      901 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
      961 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1021 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1081 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1141 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     1201 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     1261 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     1321 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     1381 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     1441 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     1501 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     1561 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     1621 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     1681 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     1741 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     1801 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     1861 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     1921 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     1981 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2041 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2101 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     2161 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     2221 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     2281 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     2341 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     2401 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     2461 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     2521 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     2581 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     2641 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     2701 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     2761 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     2821 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     2881 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     2941 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3001 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3061 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3121 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     3181 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     3241 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     3301 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     3361 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     3421 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     3481 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     3541 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     3601 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     3661 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     3721 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     3781 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
     3841 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     3901 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     3961 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4021 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4081 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4141 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     4201 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     4261 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     4321 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     4381 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     4441 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     4501 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     4561 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     4621 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     4681 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     4741 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     4801 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     4861 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     4921 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     4981 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5041 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5101 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     5161 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     5221 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     5281 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     5341 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     5401 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     5461 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     5521 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     5581 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     5641 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     5701 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     5761 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     5821 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     5881 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     5941 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6001 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6061 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6121 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     6181 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     6241 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     6301 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     6361 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     6421 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     6481 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     6541 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     6601 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     6661 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     6721 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     6781 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     6841 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     6901 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     6961 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt
     7021 ttgttgattc tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt
     7081 aaaatttcgt aaattcatat gg
//
LOCUS       DAHEYX010000142         6958 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHEYX010000142 DAHEYX010000000
VERSION     DAHEYX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.143"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(397..3102)
                     /locus_tag="MB219_002032"
     CDS             complement(397..3102)
                     /locus_tag="MB219_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCC8427743.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3408..4355)
                     /locus_tag="MB219_002033"
     CDS             complement(3408..4355)
                     /locus_tag="MB219_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCC8427744.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4545..4814
                     /gene="rpsN"
                     /locus_tag="MB219_002034"
     CDS             4545..4814
                     /gene="rpsN"
                     /locus_tag="MB219_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCC8427745.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4990..5352)
                     /locus_tag="MB219_002035"
     CDS             complement(4990..5352)
                     /locus_tag="MB219_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCC8427746.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5363..6484)
                     /locus_tag="MB219_002036"
     CDS             complement(5363..6484)
                     /locus_tag="MB219_002036"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCC8427747.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6500..6850)
                     /locus_tag="MB219_002037"
     CDS             complement(6500..6850)
                     /locus_tag="MB219_002037"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCC8427748.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         1848 a         1492 c         1273 g         2345 t
ORIGIN      
        1 ttgaattcta taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga
       61 tatcttaact tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg
      121 aagcatagcg cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg
      181 taataagctc aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac
      241 ttgatgcaga gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc
      301 ggtattagct caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat
      361 actcaggtca aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg
      421 cgccgcttct ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac
      481 gattccaaag acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca
      541 ataaattcct cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg
      601 attgatcatt tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga
      661 gatcgacagt actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc
      721 caagacatag acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta
      781 gaccggattt tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct
      841 ttgcgcaatc gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt
      901 aaaattgttt cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag
      961 aacagcgccg acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt
     1021 tgcgattgga taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc
     1081 ttttgttgcg tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc
     1141 ttttcggatc gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt
     1201 gatcaagtcc gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt
     1261 tgtcaatgta ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc
     1321 ggccaatcct aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc
     1381 attgatgcca ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat
     1441 ttcttgtcca attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt
     1501 tacagttgct ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag
     1561 tgcgccttgt gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga
     1621 actgatttga cctgtattga taccagcaat gatgccattg atattattaa caacggcatc
     1681 aatttgatct agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa
     1741 agcagatagg cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat
     1801 aattggctgt aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag
     1861 tttatcgatt gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct
     1921 gatgtttttc ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc
     1981 tattaacgaa cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact
     2041 gtttgtgatg cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc
     2101 tagatcactt gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg
     2161 gatgatcgtc aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga
     2221 agcaaaatcg ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt
     2281 ttcttgcaaa tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa
     2341 tgcaacgact ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc
     2401 tttattcagt ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt
     2461 gtagccgtcg atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt
     2521 gatacttact aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt
     2581 cgaactggct gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt
     2641 atcggtgatt ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg
     2701 tttcttgata tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta
     2761 aatacctgcg taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac
     2821 gaattgccat cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc
     2881 cacttcttta ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc
     2941 tccagtattt ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg
     3001 gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca
     3061 gtcaagcaaa tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct
     3121 tttttcagct gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa
     3181 aaagtcgaat ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag
     3241 aaataattaa ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat
     3301 gccaaagttt ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc
     3361 ccctacaaaa atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat
     3421 gacaggatga ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca
     3481 tgcttaagtg aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag
     3541 gctttgatta tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat
     3601 tcataaaagc cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca
     3661 tacctgccgc ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc
     3721 gcatcgatta caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa
     3781 gcagcaaatg ctgaagctcc taattttttt accttttcct catttttact ggatgcactg
     3841 gttaagattc ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct
     3901 gatgctccta gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga
     3961 ttataagccg taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct
     4021 ggaatttttg ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact
     4081 gcatggacag ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc
     4141 acaataccag cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc
     4201 atttctccta aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca
     4261 cgaacatgtc cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt
     4321 gcttctccgt aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa
     4381 gtttacgttt atgtctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc
     4441 aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa
     4501 gattacgatt tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa
     4561 agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg
     4621 aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag
     4681 aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg
     4741 ggatgtcccg tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa
     4801 aagccagctg gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc
     4861 cttggatcat caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca
     4921 gacgtatctt tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact
     4981 tcgctatatt tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc
     5041 tcggtcaact tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga
     5101 tttatcgatc gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc
     5161 agtagctttg atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc
     5221 cgcaacaatg atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg
     5281 gcgtgttccg ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc
     5341 tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc
     5401 cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt
     5461 ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca
     5521 atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta
     5581 ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg
     5641 ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg
     5701 ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat
     5761 aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa
     5821 tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat
     5881 atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc
     5941 aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta
     6001 atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc
     6061 cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac
     6121 ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata
     6181 aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg
     6241 gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc
     6301 ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt
     6361 tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta
     6421 cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa
     6481 ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg
     6541 tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac
     6601 cattttcatt ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc
     6661 aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat
     6721 gaaaaggcaa ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt
     6781 ttggtttttc ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc
     6841 cttttatcat atgcttttct cttttcaata aatttagaac actctcattc taccaaatta
     6901 taaccatttt gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaa
//
LOCUS       DAHEYX010000143         6942 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHEYX010000143 DAHEYX010000000
VERSION     DAHEYX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.144"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            182..1039
                     /locus_tag="MB219_002038"
     CDS             182..1039
                     /locus_tag="MB219_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCC8427749.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1032..1934
                     /locus_tag="MB219_002039"
     CDS             1032..1934
                     /locus_tag="MB219_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCC8427750.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1967..2317
                     /locus_tag="MB219_002040"
     CDS             1967..2317
                     /locus_tag="MB219_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCC8427751.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2366..3288
                     /locus_tag="MB219_002041"
                     /pseudo
     CDS             2366..3288
                     /locus_tag="MB219_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3366..4259
                     /locus_tag="MB219_002042"
     CDS             3366..4259
                     /locus_tag="MB219_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8427752.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4297..4860
                     /gene="amaP"
                     /locus_tag="MB219_002043"
     CDS             4297..4860
                     /gene="amaP"
                     /locus_tag="MB219_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCC8427753.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4874..5062
                     /locus_tag="MB219_002044"
     CDS             4874..5062
                     /locus_tag="MB219_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCC8427754.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5075..5977
                     /locus_tag="MB219_002045"
     CDS             5075..5977
                     /locus_tag="MB219_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCC8427755.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6009..6251
                     /locus_tag="MB219_002046"
     CDS             6009..6251
                     /locus_tag="MB219_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCC8427756.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6507..6878)
                     /locus_tag="MB219_002047"
     CDS             complement(6507..6878)
                     /locus_tag="MB219_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427757.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2530 a         1184 c         1386 g         1842 t
ORIGIN      
        1 tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt
       61 gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca
      121 aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt
      181 gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc
      241 ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa
      301 attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt
      361 aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga
      421 agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa
      481 cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact
      541 agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat
      601 cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt
      661 caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa
      721 aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt
      781 gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc
      841 gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg
      901 aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa
      961 agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt
     1021 aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg
     1081 taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg
     1141 ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct
     1201 cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct
     1261 ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc
     1321 cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg
     1381 ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg
     1441 ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc
     1501 gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta
     1561 cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc catgaccaag
     1621 atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac
     1681 aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg
     1741 aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca
     1801 aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc
     1861 gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg
     1921 tagttgttaa ataatagaag acaacaactg aagaggggga gtaggaatgg aaattacaat
     1981 gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact
     2041 cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag
     2101 cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca
     2161 tccattattg gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa
     2221 catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga
     2281 ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag
     2341 gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga
     2401 aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat
     2461 cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc
     2521 tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa
     2581 tctagatcag tcagataatc gtatcgaaaa aaattaacgc caatatcttt tgtactttcc
     2641 gataatctct tgattacttg tatcgataga aaaaccgaat ttgtggatcg gctgctgaca
     2701 aagcatggaa atcatttaaa ctcgtttgaa aagatcatca cgtatacatt attagatatt
     2761 tacacccatt acgtgaaaga actaagagag atgaaaaagc gaattgacgc tttggataaa
     2821 gcagctcgta aaacaacgga aaatgaagaa ctgtataaac aggcagatct tgaaagagac
     2881 atcgtatata tcgatcatac cttaagagat caacgggaaa caatggatca attatggaat
     2941 tctgaaactt tcatcaaacg tttagacgat gaacagctac tctatgatgt ccatttgaga
     3001 cagcgacaaa cagaaaaaat gatcgagatc tatcgagatt tattagagag tattggtgat
     3061 ctattcaacg ggatgatgga caataatcta aatcatatca tgaaatatct agattcagct
     3121 gcattaatca tttccgttcc ggcgttgttc gcagggattt ggggaatgaa tacaggagga
     3181 ttaccgggta aaagttctgc tctaggcttt atactcgtag tctttggctc tttgatagca
     3241 gcagtagttt taggttacca tttataccgg aaagattata caaattaaag aatctattta
     3301 aaacaggtat caaacaaaga aatttgtttt ggcaatacaa tttaaaaact aggaggaata
     3361 cagcaatgac agaaccaaaa ttaatcgacc cgcgaaactt gtaccatacg gagaaatttc
     3421 ctaaacaaga tcaggataca ccagctttac agcagaatat gattcctgtt cctgattgtg
     3481 gagaagaatc ctatgaaggc aataatcaac tagaaaaccg ccgtgtacta atcactggtg
     3541 gagattcagg gatcggtcgt gcagcagcga ttgcatttgc tagagaaggg gcagatatcg
     3601 ctcttcaatt cttcccagga gaagaaaaag atgcagagga agtagcaaaa tacgtgaaag
     3661 atgcaggacg taagattgtc ttactacctt ttgatcttcg tgacgaacaa gcacctaaag
     3721 aaatcataga taaagccgtg agtgaacttg gcgggctaga tacgatggta ctaaacgcag
     3781 gacagcaaat ttcttgtcag tctattgctg agttaccact tgagcaagtc aaagacacat
     3841 ttatgataaa catcatcgct atgtttgctt tagtgaaaga agctgttccg catatcccag
     3901 caggaggagc aatcgtaaca acaacttctg ttcaagcatt caatccaagc gaacatttat
     3961 tagattacgc agcaacaaaa gcttcaatcg ctaactttac gatcggttta gcaaaacagc
     4021 ttgcacctaa aggaatccgt gtaaatggtg tagcacctgg tccaatctgg acaccattac
     4081 aactagacga aggacaacct tctgattcga tccctgaatt tggtcagcaa agtttactag
     4141 aacgtgcagg tcagccagga gaattagcac cagtttatgt tttcttagca tcgaataaag
     4201 caagttatgt tacagcccaa atttacggag taactggtgg agaagcgatc aacttataag
     4261 aagttgatcg tttcacccct atgaggaggt gcgaaaatga ataaaggtgt aaaaacgatc
     4321 ggagtgatcg gaacattact tttgctatct gtgcttttgt ttgcgatact attaagtgca
     4381 acctatccat tgccttttca gctagagaag tttcgattct tttccatcac taattcatac
     4441 gtgcaacaat atgtattttg gcttgccgtt gcatttgcta ttttgacaat tatcacgtta
     4501 cttgttttcc tgttttatcc aaaaacaatc ggaaactttc tactgaaaca tggtgatggg
     4561 gagttgacgt tggataaaaa agcaattgaa ggaatggttc gttctcactt gcatgaagat
     4621 gaattcatcc attcaccaaa agtaaatgtc aaagcaacga aaaacaagat cgccgtcaat
     4681 gtaaaagggg agttgaaacg aacttcagca ctcattggta aaacaggaac attgatgaaa
     4741 gaaatcgaaa atgaagtaca acacatattg ggaacaaaag agccagtgaa agtatcggtg
     4801 aaatacacag gctttgaaga accagctaaa cgaagcaatg accgcaaacg agtagaatag
     4861 ggggggacca aaaatgaaag aactattttc gacatacaag ctccctatca tctttggggt
     4921 acttggattg atattagccg ttttatggat caccgtgggc tttttcaaga ccttattgat
     4981 cttgatcatg gcagcgatcg gcgtcgctat cggtttttat ctgaaacaaa cgaaaatctt
     5041 agatcactat tttaaatctt aggaggaaac atccatgaat aacaataaca aaaataataa
     5101 caataaaatg aacaaccaaa aaccagtgtc tgcacatcca gacccaacga caccaaacca
     5161 atcaaatggt gtgaacgatg aaaagaacaa aacagcacca gtatctgcac atccagatcc
     5221 agtagtccct aacgttcaac aaggagtaga agagaagaaa catgatgcag aaaagcatgc
     5281 acctcatcat ccaggaactc cagaatctaa agttccagaa caacatgatg caatcgatcc
     5341 ttctaaacct gtggaagagc aaaaacatcc ttctgaccat gcgaaagcac aagaaaaact
     5401 tagttctgac cacacaaacg gacaaggaaa acttggttct gatcatacaa ataaccaagg
     5461 aaagcatgat tctgaacagg aagattcaat gcgcggtgag ctgaattatg aagataaagt
     5521 tgtgcaaaaa atcatcggaa tcgcaattga acaagtagac ggtttattat ctgcaaacgg
     5581 tggtttcttc tctaatgtgg caggaaaact tgtgaataca gataatgtaa cggctgggat
     5641 cgaaacagaa gtcggtaaaa aacaagtagc agttgatatg gatgtcattg tggaatatgg
     5701 caaagatatc gaaaaaatct ttgaagaaat gcaagaagta atcggcaaag aagtaaaaaa
     5761 tatgacacat ttagaagtga tcgaagtcaa cgctaacgta gtggacatca aaacaaaaga
     5821 agaatttgaa aaagatcaag aaacggtgca agacaaagta actgacgcag cgaaaaaaac
     5881 cggtgaattt gcttcgaatc aaacagataa agtaaaatca gcagctggaa gcggcgcaca
     5941 aaaagtaaaa gaaaatacag aaccacgggt tcagtaataa ggaagtaaaa cattaggagg
     6001 aacgaaacat gggatttatt tggtcactta tcgtcggagg aattatcgga gcaattgcag
     6061 gagcaatcac gaataaaggt tcttcgatgg ggattattgc aaatgtcgta gcaggtttag
     6121 ttggatcagc acttggtcaa gcattattag gttcttgggg accaagtatt gcaggaatgg
     6181 caatcgtgcc atcattgatt ggtgccatca ttttagtact aatcgtatca ttctttgctc
     6241 gaaaactgta aaaagacttt taaaaagagg tcgtgaaaaa gctgttcttc atcaagtaat
     6301 aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttgggca tattatcgaa
     6361 gatgcaagct tttgaacact gtttatacgg aataagaggc tgtgacaaga cttttgtcac
     6421 agcctcttat ttatgcgggc aaattcccaa ttagattttt aaaagttttt ccttaaatgt
     6481 gtctttattg atacagagaa gcttctttaa taaaacaatg cctccctaaa ttttcttgag
     6541 ataggaaagg tgaacataca aaataatacg accactttga aaacccagat gatccacaaa
     6601 tggatcgtat gatactgatg aagtaaaaag caatggatca gaaactcaaa gagattgaaa
     6661 aagacaagga agaaataata tctctttttt actcttgtct gttttagcca ataatagctc
     6721 gtaaagtaat ctagacaaac aaatagaagt aaacaaagtt tcgtaagagc agcaatcgta
     6781 tcatgttgct ctgctagacg attgacgagc aggaaaaatg ttggtaatgg aatatagatc
     6841 acgagtaatg aaagaaaaaa gtaaacaata aatggcatac tattcctcct aattgtgtga
     6901 gttcgccctc attttaaaat aacgatattt gtttgtgaag ct
//
LOCUS       DAHEYX010000144         6817 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHEYX010000144 DAHEYX010000000
VERSION     DAHEYX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.145"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            50..940
                     /locus_tag="MB219_002048"
     CDS             50..940
                     /locus_tag="MB219_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCC8427758.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1013..1837)
                     /gene="nadE"
                     /locus_tag="MB219_002049"
     CDS             complement(1013..1837)
                     /gene="nadE"
                     /locus_tag="MB219_002049"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCC8427759.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1849..3321)
                     /locus_tag="MB219_002050"
     CDS             complement(1849..3321)
                     /locus_tag="MB219_002050"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCC8427760.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3958..4320)
                     /locus_tag="MB219_002051"
     CDS             complement(3958..4320)
                     /locus_tag="MB219_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCC8427761.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4543..5295
                     /locus_tag="MB219_002052"
     CDS             4543..5295
                     /locus_tag="MB219_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCC8427762.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5435..6358)
                     /locus_tag="MB219_002053"
     CDS             complement(5435..6358)
                     /locus_tag="MB219_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8427763.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6373..6645)
                     /locus_tag="MB219_002054"
     CDS             complement(6373..6645)
                     /locus_tag="MB219_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427764.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2088 a         1353 c         1163 g         2213 t
ORIGIN      
        1 ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata tgcaactaca
       61 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
      121 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
      181 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
      241 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atatcagttt
      301 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
      361 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
      421 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
      481 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
      541 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
      601 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
      661 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
      721 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
      781 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
      841 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
      901 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
      961 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
     1021 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
     1081 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
     1141 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
     1201 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
     1261 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
     1321 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
     1381 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
     1441 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
     1501 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
     1561 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
     1621 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
     1681 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
     1741 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
     1801 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
     1861 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
     1921 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
     1981 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
     2041 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
     2101 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
     2161 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
     2221 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
     2281 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
     2341 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
     2401 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
     2461 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
     2521 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
     2581 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
     2641 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
     2701 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
     2761 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
     2821 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
     2881 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
     2941 tactaattgg cagtgcgcta aaggcccttc cacttggatt aatggttcat ttgcaaaaac
     3001 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
     3061 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
     3121 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
     3181 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
     3241 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
     3301 gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca tttcctaccc
     3361 attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa aaagcacatg
     3421 ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc tttcttattg
     3481 gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag acacgaagaa
     3541 attagcgttt tctatttttc ttacttgata aaataagtag attttcctac tacaatcgta
     3601 gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac ataatcaatg
     3661 gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc ccaaagaaac
     3721 ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca agatatacct
     3781 tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac aaagtaagaa
     3841 agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct cgccttgttt
     3901 tttattgtat acattagata tggagcaggt taccataaag atgattatta gtcgttatta
     3961 aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg gacgtttgaa
     4021 ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga catcttctcg
     4081 tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga tttttttctt
     4141 cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca gttgtgttcg
     4201 atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga attccactac
     4261 tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga ttagcttcat
     4321 ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt taccatcttt
     4381 cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt taccttttat
     4441 cacttaatag tataacataa tttcgtgaga aaaaaataca attttttctt gaccatctcg
     4501 ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta cacaataaag
     4561 aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga tgagattgga
     4621 ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa aaaagaagtc
     4681 gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga tgatatccaa
     4741 gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca tcagcaaatg
     4801 ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca gacacttgat
     4861 atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa
     4921 aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata cggaaaaaaa
     4981 aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta tgaagaaatg
     5041 caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga accagaagat
     5101 gaaagcttag cagaccgtat cttccaagct cataagaagt ggttctctta cagctggcct
     5161 gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga tgaacggttc
     5221 actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc
     5281 caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa atgacctaat
     5341 aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc tgctccattc
     5401 tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac gtataaggta
     5461 ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct ccgcaagctt
     5521 ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc
     5581 caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct gcaaccatat
     5641 ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct gcttgttttc
     5701 ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt
     5761 gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt cgccccaacg
     5821 tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct gtaattgatt
     5881 gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca caaaaaacta
     5941 gaggtccata tttttgatat gccaataaat catctagtgt attggcacga gacgtaatga
     6001 tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct ttttctttat
     6061 gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct tcaatgatcc
     6121 cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact ccaatgcttt
     6181 tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg acttctttca
     6241 tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca ttgattacac
     6301 gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg tttgccactg
     6361 ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag ctggaccgca
     6421 ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc cgccccaata
     6481 tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta tattcctatt
     6541 caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa acgcataaaa
     6601 ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa gccctccttt
     6661 tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt tttgccaaag
     6721 aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg aatacattat
     6781 aatgatactg taaatatatc tttattatta gtgaatg
//
LOCUS       DAHEYX010000145         6799 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHEYX010000145 DAHEYX010000000
VERSION     DAHEYX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.146"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            130..1140
                     /locus_tag="MB219_002055"
     CDS             130..1140
                     /locus_tag="MB219_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCC8427765.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1209..2153)
                     /locus_tag="MB219_002056"
     CDS             complement(1209..2153)
                     /locus_tag="MB219_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCC8427766.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2137..4857)
                     /locus_tag="MB219_002057"
     CDS             complement(2137..4857)
                     /locus_tag="MB219_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8427767.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4854..6074)
                     /locus_tag="MB219_002058"
     CDS             complement(4854..6074)
                     /locus_tag="MB219_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCC8427768.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6355..>6799)
                     /locus_tag="MB219_002059"
     CDS             complement(6355..>6799)
                     /locus_tag="MB219_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCC8427769.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         2007 a         1289 c         1108 g         2395 t
ORIGIN      
        1 gtatttccta ggaatgtgat gaaattttga attttgtttg aaactaccac gacaacgttt
       61 ttaaaggaaa tgggatatgg tatagttaat atataaaaat aataactaat tacaggagtg
      121 caaattttaa tgaaaaaaaa atcaattatt ttggcagcta ctagtgcgtt ggctgtttta
      181 actctagcag cttgttcagg cgatactaat aaagatatcg caacgatgaa aggcggaacg
      241 atcactgtat ctgatttcta tgatgaagca aaacttgaat catcgaacca atccttagta
      301 caacggatga tcatttataa agtattcaat aacaaatatg gcgataaagt aaccgataag
      361 caagtggatg cagaatacga caaacaagcg aaatcattag gtgatacttt tgaatcacaa
      421 ttagaagctg ctggatatac gaaagatact tataaagaat atattcgtaa caatttagct
      481 tttgaagctg gcttgaaagc tcacgtagat attacagatg acgatttgaa aactgcttgg
      541 aaatctttcc atccagaagt agaagcacaa atcatcaaat tatcaagtga agatgaagca
      601 aaagatgtga aaaaatcagc ggatgatgga gatgatttct ctaaattagc caaagacaaa
      661 tcaactgata cagagacaaa agaagatggc ggtaaagtta aattcgactc aacgacaaca
      721 actattcctg cagaagtaaa agaagcagct ttcaaattga aagacggtga gatctccgat
      781 gttattacta caacaaatcc tacttcttat gcgacagaat actacgttgt caaaatggtg
      841 aaaaaccaaa ataaaggcaa tgacatggat aaatacaaag atcaactaaa agatatcgct
      901 actgaaacta aactaagtga caatgcattc acaactaaag taatcggtga agaattaaaa
      961 gatgcaaatg taaaaatcaa agatgatgcc tttgaaaatg tgttgagtgc attcacaaca
     1021 acttcatcaa gtacgaaaga ctcaagtgaa actacagctt caacaaagag ttctgacaca
     1081 aaatcaactg attcaacaaa agaatcttca actgaagaaa caactgattc atcaaaataa
     1141 aagaaaacaa aaaaagcgaa gacttatgtc tcgctttttt tgttttgaat gaaaaaaata
     1201 aatttttgct ataaatcttt tggtacatag aaacttcggt tgtccatacc gaaaatccga
     1261 tcggtgtatt ttcctggttc tgtttgacga atcgaagtca acatcatctg catcgaagca
     1321 tcgatattgt cgatttgatg taatatttct gcttccatta ttcttggacg tacaggtgag
     1381 ccatattcta ataagccatg gtgtgctaaa accatatgac gtaatacgag aacatcttcg
     1441 tcagcttcgc tgattttcaa cgcaagacag gctttagtta tttcttcgtc aatcaatacc
     1501 agatgaccga tcaaatttcc tgcaagcgta tattctgtcg acataggacc ggaaagttca
     1561 atcacctttc ctaaatcatg caaaatgata ccagcataca acaatgctcg attcagctca
     1621 ggatattcat cggcgattga tttccctaag tgaagcattg tcaaagtatg ataagctaat
     1681 ccactggcaa atgcatgatg gtttcttttt gctgccggaa actcaaaaaa ttgtttttga
     1741 tatttattaa gtagatagcg aacaatacga ttccaatgcg cattggtgat ctcaaacaac
     1801 aattgattga tttcttcttc catttcttct ttcttcaaag gggctctttc catgtataaa
     1861 ctaggctctc ctggctcatc tacccttgtt aagcgcatat gaagaatctt tacttgaggg
     1921 tttccttgat aaacctctct tttaccgttt agaaaaacga ctgtgccggc tgtaaattta
     1981 ttgatttcct cctccgaagc atcccagtat ttaccatcga tagtacctga agtatcttgg
     2041 aaagtaaaag caataaattt ttttccgttt ttagcgattc ttacatctgc acttttgatc
     2101 aggacaaatt gttcaaactg ctcatcgatt actaaatcac gaattttttt cattatttgc
     2161 tccttctaat tgttttacga tttggttcat ctcatggtaa tagcttacca tttctttatc
     2221 agaagaaaaa caaattattt gatacttttc tccaaattta gctaaaagtt cagctaattg
     2281 atactttcgc tgatgatcat aatgaagcca gccatcatca atgatgatcg ggcatatcga
     2341 acggttagct tctaatgaga ggtaagcaaa tctcatcgcc ataatcagct gatctttcgt
     2401 acctgtagat aactcataga tgggaaattc tgtatatccg tctgttaaag tcagtgtatc
     2461 atttttcagt aatatcttct gataatttcc ttttgttaaa atacctaaat aatcacctgc
     2521 tgctcttaac aactggggta attgtctttc actcatctct gttgaaagat cacctaagaa
     2581 tgtcccggcg atttcaagtg ctccccattc ctccagtaat tcctgtaatt ttgctttttg
     2641 ccggctagct tgttgataaa gttcgtccaa tgtgccgtct ctttgcaact cttctacttg
     2701 aagttggagt cgttgccgat tttcacttgc ttctttttct tcttgctgga gttgggattg
     2761 cttttctttc agttggcgca gtcttacaga taatttctct tttgtcagct cttcaggaaa
     2821 tagtggagta agcatttccg ataattcatt gtatcttttc atctgctgtt cggcttgttt
     2881 ggctttttgt aaaaagacag gtacttccga aggataagat aagcctgctt gagtcaattg
     2941 ttcgctgtat tcttgtatcc atactttttg tttctttttc aattgattga tttcttgctt
     3001 caataaagta ttttgctgat agcttctggc aaatttcatc tgttccatct cttgtataaa
     3061 acgggaaagc aataccattt tttctgaaat tgtcttatct tgtatcggta accattcttt
     3121 aacaaactca aatttttggt cgatttgtcg tagtttttct tgtatttcct gttctctttt
     3181 atgtttccgc tcaaatacgc catataattt ctgataatct atgacttcct gtccatattc
     3241 tttcatgagt ttgagcgtat ccatctttcc taagtgccga tgatccgcaa aacgatatac
     3301 ttcttgctga agaatctgtt tttcttctcc agttctcttt tgtttttctt ctgcttcagc
     3361 taattgagct aaggctagat caagttgtcc tagtttttct tgccacattc cttttatctc
     3421 agagaaatca tttttagttt taatttttgg ttgccttttg tagaagaaat agccaatcat
     3481 gcctgcacca attagtaaaa ggatcacttt caagggcatt ggtacaaata tacctgcaat
     3541 gattattgct ccgccagcca tcagcatata cggtttagat gaagagactg gtgctatctc
     3601 tgactgattt tgtaataatg ctggataggt ctcttccaag tcattcactt ctgcttcaat
     3661 catctcttgc tgttctttag ctatttggag acgcacagaa atctggcttt ctagatcagc
     3721 aatccttctt tcttcagcca gaagatgctt ccattcttct tcatataaaa attcttccgg
     3781 taacggattt ttctgccaat gccacctttg ttccaaaatc ttcaattctg aatttgtttc
     3841 tctagtctca atttgtaatc tttgccattc ttcgtttaat tgttccgctg taaattgaag
     3901 ttgctggaga tcttttagtt cctgctcctg ctctaaataa aaataatatc tggctgactg
     3961 ctgatccatc ccgctatgct tttccagtgt tgcttctaat tcttccaaac gctcatttgt
     4021 ttgctgatac cgtttggaga aagactccag atctaaaaga agttgctcat caggaagacc
     4081 tgtagtgctg ttttttttga gagattgcca ttcttcatat aaagaaaaat taagttcttg
     4141 ctgtctggct aatgaaaatt gtttgttcgc ctcttttaat tgtgccaata actgctgctg
     4201 ttgcgactcg tattcctgtt cttgttggat caacttgcca aaataagctt cctgttcttg
     4261 tttttgatga attttttctt gcagctcttt ccattcattt aatttttgat tgattagtaa
     4321 tttttggcct tttttcttga ataattgttg tgctttctga taatattctt gccgcttttg
     4381 gaatacttga gaactgcccg tgatgcctaa agaaatcagc gcatcatgta gctctttttc
     4441 tctcaaatga tctagttcca tcaactgctc ttgctggaaa gtaaatacct gctggaataa
     4501 ctctttcgtc aaaggtgcga tcagtttttc tagtaactca tcacttccca cttgatcccc
     4561 cagccacacc ttcgattttc cgcgattttg ctgcttgtag cgctcgacag caaattcacc
     4621 atatacagga tgtttaagcc atattttgcc gccataggct gagccgtctt ttggtgtata
     4681 atcctttttt ttacggcctt ttgaagggaa accaaataat attgcttgaa taaattgata
     4741 aatcgttgat tttcctgctt cgtttccccc atagatcagc tgattgcctg aataaaagtc
     4801 aaaacttttt tgacgccatt ttccaaaagc agcaatctcg atccttaaaa tcttcattcc
     4861 tgatcctcct caaataaaaa atcttgatta agcagctgct tggctttttc aagtgtttct
     4921 tcttgatatt ccggcagttc gctcaatatt ctagccgctt cttgatgact atatacttca
     4981 tttaaaattt gttggaaatc ttgaggtgtc tgatagtatt gaaataactg ttccatcaat
     5041 ttagcagaag cagcaagtgg ggtcttcgta taaaaagtat tctctatgag agaaatacgc
     5101 caaataaaga aatcatttat cctatctgag aattcctcta acaaatagtc taatagctct
     5161 cctgattgta tccgttccaa aacatctgta ttgagatggt cataatcttt taattttatc
     5221 tcaattaaac taaatccatc ctctactttt gaaagctgct gacgaatgtg aacaatgacc
     5281 tcttttgtcg ttcttgcttt cctcaatgat atttcctttg tcttccaata gacctctgct
     5341 actttaatcg gatgtatttg gcaacttcct ttacttaatt caactagtag aatagatgta
     5401 gaagcttgtt cctttttagt atgaccttga ggcgcacctg gataaacaat tgtttgcttt
     5461 tcatttaaga ccgttggcac atggatatgc cctaaagccc aataatcgta tcccttttct
     5521 tgcatctttg agggttggaa aggagcatag tttccttttt gagagacgcc aggttccccg
     5581 tggtacaacc caatatggta atctgtgagt tctctagaag gaaattccat cactttgtct
     5641 ttttgaatcc acggttgacg ataactaaat ccactgagag agacagtttc tccacttttt
     5701 atcgttattt tttttgtctc taccgtttcg gatgtaaata aatggacatt tttaggaaag
     5761 gagaaccaat accgctcttt ttgatagaaa tcatgatttc cgaatattat aaatacaggt
     5821 atatggttct tttctaaccg tttcatctgt tctgaaaaat gtttctgtat ttttaatgaa
     5881 ggacgatttt gatgaaaatt atctcctgca agtaaaacaa aatcaacgtt atttattatt
     5941 gcctgttcaa tgatatttga taaaacggtc agattcgtct tcaatagttt ttcctgtaca
     6001 gttttatcaa gagttgtcag tccttcaaat gaccgatcca tatgtaaatc tgccgcatgg
     6061 ataaatttca ccattaaaaa tttcctcctt tttttctttt atcataccat tttcaatgtt
     6121 ttttttcatc aataaccgaa aatttgttcg tattatgcaa agaaattacg ataaatggcc
     6181 gacttcaaaa aataagttta ttcgaaaaaa agattgtgac atgatttttg tcacaatcct
     6241 aaatatctat gttatattcg cttcgtttga cactgaagaa gtactatttg tactggaatt
     6301 tatgctccat ttttccgtat ttagaattct atgttctata ctaccttaat tagtttatat
     6361 ggtaagaatt ctttcaaatg cttgagatac tccgtcttta tcatttgaaa cagtgacata
     6421 atcagctttt ttcatgattt cttgatgact atttcccatt gcaaaactaa tatctgcact
     6481 ttcaaacata gataagtcat ttaaactatc tccgatcgcg attctcaaag gtttctttaa
     6541 attgtagatt gattccaaac gatcaaacac cacaccttta gatacatttt gaggtacgat
     6601 ttcaatattt acaggtgccg attcagtaac agacacattt agcatctttt tcaaatggtt
     6661 aaatatatta gctttcaatt gacgattagg aataaaaata aaaaatttgt aaatgctgat
     6721 agcatccttt tctataattt cctcaatatt attataaggg attgaacaaa tagatttttc
     6781 atactcaagc tcatagttt
//
LOCUS       DAHEYX010000146         6757 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHEYX010000146 DAHEYX010000000
VERSION     DAHEYX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.147"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..179
                     /locus_tag="MB219_002060"
     CDS             <1..179
                     /locus_tag="MB219_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427770.1"
                     /translation="IPASAEDIAIPDSLIIYLDETESEVATDVHVVEDKNDSLKINSS
                     DTLLRVSPIFSAVI"
     gene            488..682
                     /locus_tag="MB219_002061"
     CDS             488..682
                     /locus_tag="MB219_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427771.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            840..1122
                     /locus_tag="MB219_002062"
                     /pseudo
     CDS             840..1122
                     /locus_tag="MB219_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1366..2196)
                     /locus_tag="MB219_002063"
     CDS             complement(1366..2196)
                     /locus_tag="MB219_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCC8427772.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2404..3738
                     /locus_tag="MB219_002064"
     CDS             2404..3738
                     /locus_tag="MB219_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCC8427773.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3779..4690
                     /locus_tag="MB219_002065"
     CDS             3779..4690
                     /locus_tag="MB219_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8427774.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4687..5541
                     /locus_tag="MB219_002066"
     CDS             4687..5541
                     /locus_tag="MB219_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8427775.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5538..6155
                     /locus_tag="MB219_002067"
     CDS             5538..6155
                     /locus_tag="MB219_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCC8427776.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6157..6675
                     /locus_tag="MB219_002068"
     CDS             6157..6675
                     /locus_tag="MB219_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCC8427777.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2025 a         1178 c         1362 g         2192 t
ORIGIN      
        1 ctattccagc gtcagctgaa gatattgcaa ttcccgattc cttaatcatt tatctggatg
       61 agacagaaag tgaagttgca acagatgtgc atgtagttga ggataaaaat gatagtttaa
      121 aaattaatag ttcagatact ttactaagag taagtcctat cttttcagct gttatttaga
      181 tgcaagtagc aactaagtta ggtagtactc ctgcaatgga taggtatttg tgtgttcgat
      241 atataagtgg tcgaagtatc aaggttatgt atactggatg ggagatagaa gagttaaaaa
      301 ttggaggtca ccgccacaaa tggaatttag atttagaggt accttagatt tggtatagtt
      361 attaaacaca agatggcctg gtttgtaggg aaagtcgaag agtatttttc cacttctgcg
      421 cccctttaat ttttctagta atgccgtgaa tttgtgatta attggaaata tgaagaaagg
      481 agagtctatg aaaacaacac tagttctatt ctataaaaag catccttatt ttacacttct
      541 gataaatata ttgttggcta gtgttatcgg gatttctgtt gaatatctaa taaataaaga
      601 tttcataggt tcttgttttt atacggcttt atttcttgga cttcttgaag cattttccat
      661 ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca tttcattttt
      721 ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta ttgtctttag
      781 acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa
      841 taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt gtattcacac
      901 caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag gagatcatca
      961 agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt ggaaggatac
     1021 tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca agaacaagaa
     1081 aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc ttttaagggc
     1141 taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc aatatggctt
     1201 gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga gcttatagct
     1261 ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc agttttgctc
     1321 acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt tcttgaatgg
     1381 aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt
     1441 aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac tagcccaaga
     1501 accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa tgtcttttcg
     1561 ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt cttctaattc
     1621 tttcactgca cattcccata gattttgatg atagctatct cttgcaagga gaacagacag
     1681 catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt gaagtcttcc
     1741 ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac tcgcaatgat
     1801 ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta agccagctac
     1861 gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat ctttttgcat
     1921 cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa
     1981 catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt tatatttttc
     2041 ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca tctcgccata
     2101 ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac gatctggttt
     2161 tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta atctattttt
     2221 aatacattct cattctgcta cgaaagatta aaaaattcaa agaaagcgct cgcgaattat
     2281 ataaagtatt ggtatcatta ataatttttt tagaatcata ccttataaat tgttattttc
     2341 agaacaaaat gatatcgtta tattaaaacc ggcatcggtt acattaaaat ttggaggtaa
     2401 aatatgaaaa aattattgtt tacagggttg ctggcaagtt cagcattgtt tttattagga
     2461 ggatgcggat caagtaatgc aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa
     2521 acaagtgatg gaagaaaaaa tattgatttc tggtcctttt ggggatccgg tgcacgaaaa
     2581 gaagctatcg aagaaatcat tgaggatttc aatcaatcac aaaacgagat ccatgtaaat
     2641 tataagtatc agccatgggg tgacatctgg actaaatctt tatctgcaat aacagctgga
     2701 aatgcacccg atgttattgt acaggacata aattccgttg cccaaagagc agaagcacag
     2761 caagcaacta atctctccaa atatgtagaa aaagacactt ctaaggattt ttatccgcag
     2821 ctttgggata cagtagaata tgaaggtgat ccttatgcga tcccattcaa tacagataca
     2881 caagtcattt tttataataa aaagttattt aaagaagctg gaatcagtga acaagatcta
     2941 ccgacaactt gggatgaatt agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa
     3001 gaatttacac gcatcggttt ttatccatta tggaatttag gggctgatgt atgggcgcta
     3061 aacgcagata acgggactag ttggtttgac aaagatgaaa aagtcaagat tgatactaaa
     3121 aataaagtgg aagccttgga gtggatccaa gactggcaag attattatgg gaaaaatacg
     3181 atcaataaat tagaagccga atttgggtct ggtgtatctg atccatttat ttccggatta
     3241 gtagccatgc gtgcccaaaa tatcaattat tactctagtt tgatggaaaa tgcaccggat
     3301 gattttgatt ttggcgtgat gcagctccca gaaaaagaag aaggttcagg ccattggtcg
     3361 tggggtggcg gattcgtctt ggaagtacca tacggagcaa aagatcctga ggcctcttat
     3421 gaattcatca aatatctttc ttctccagaa gtacaagaaa aattcggcga aaaaagtttt
     3481 gatatcatgg cgagcaagac agccaatgag aatttagtga ataatgacaa cctaacggat
     3541 aaaggaaaaa tgatctatca aatggcggac gaaaacttta aaaatacggt gatcacacca
     3601 gttcctttag atgctccgga ttacacgaca ttagtgaatg aacagattga ccagattctt
     3661 ttaggaacaa aatcaccaaa agaaggattg gcagatgcac aaaaagcagt agaaaactta
     3721 gtcaagcaaa atcaataatc catttttgac tgtctaggta cagaggaggc aagaaaattt
     3781 gaagcgttta tctatccgaa agaaaaaaga agcaagatgg gcctatttat ttatctcgcc
     3841 ttttattatc gggttcttgg tatttatgct agggcctatg cttttttcag ttattggtag
     3901 tttcaccgat tataatttga catccaagat gaatttcatt ggtctagcaa atttcaaaag
     3961 aatgttcgtt cacgatgatt tattttggaa atcgttatac aacaccgttt atttcgtcat
     4021 cttcaatgta ccgctgacga cgattttcag tgtgttttta gccacagtac tcaatcaaaa
     4081 aatcaaagga atcagtgtat tccgtacagc tttctattta ccggcagtct tatcaggagt
     4141 agcagtctac atcttatgga tgcagctgct ttctccatct gcaggattga tcaacacatt
     4201 tctgggctgg ttccatatcc ctggaccagc ttggctgttt gatccacaat ggacgaaacc
     4261 tgcgttgatt ttgatgaaaa tatggagttc tggaggagca atgctacttt acttagctac
     4321 attacaaaat gttcctagat ctttatatga atcagcagaa atcgatggag caggcgtttg
     4381 gggaaagttc aaaagtatta ctttgccatt aatcactcct attatttttt acgatgtgat
     4441 cacctctacg atcggttctt tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg
     4501 aacaggcgga cctgcaaatt ctttgctttt ttataatctg catatgtgga acaaggcgtt
     4561 cgtagctttt gatatgggtt atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt
     4621 ggttctgacg tttgtcaatt tgaagcttgc acctaagtgg gtctattatt ctggaggtga
     4681 cggcaaatga tggattattt caaaaagacc agtgtgaatc ggcaaacaaa aaaaatacta
     4741 agttatgtcc tcatgacagt aataggaatc attttgatca ttccattgct ttggatggtg
     4801 tttacttcat tgaaaccaat ggaagaaatc gttcgttatc ctccgacctt tttcccagaa
     4861 aaaatcgtat gggaaaacta tctggatacg attgccgcct ttcctttttg gcgctatgca
     4921 agaaatacat tattcatcac tgtgctagta gtgatcggta atgtcctttc aaattctttt
     4981 attgcttatg gatttgcgaa gcttgatttt cctgggaaaa agctgatgtt tgctttggtt
     5041 ctttctacga tgatgatccc ggggtttgta acgatgatcc ctcaatatgt attattctcc
     5101 aagatcggct gggtaggtac ttatttgccc ctgatcgttc catcgttctt cggcaatgcg
     5161 ttcaatatat ttctgatgcg tcaattttac ttatcgatca ataatgaatt gatcgaagct
     5221 gctgaaatcg acggagcgaa tcatttgtat atttggagtc atttgatgct tccattgacg
     5281 aaacctgcct tgattaccat tgcgatcaat tccttcaacg cagcttggaa tgatttctta
     5341 ggtcctctgt tatatatcca agatcaagaa aaatacacgt tacagattgg cttgcaagtt
     5401 tttcagaacc aagcaacgac acaatggaat tatctgatgg caggagcaac acttgtcttg
     5461 atcccgacga ttttgctgtt cttttttgca caacgttatt tcattgaagg gatggatttg
     5521 acaggaggtt caaaaggatg atgaatcgat cgattggtaa aaaagccaat gattatttct
     5581 tagaaatcat tgaatggatt ccacgtctta tccatttaca gctactatgg ttcctgtgcg
     5641 tcttgcctgt tttcacatta ggaactgcaa gtcgaacagt tctgtatatg atttatcatt
     5701 acaaaaaaaa tgaagagaaa aagttcggtt cactattctg gaaaaagttt cgtgagaact
     5761 tctctcttta tggaaagcaa gatagtttag caagtgttta ttttcttctt ctattgatcg
     5821 attatcagat tttccgctat tggggtgggg gaattggcta tacattgatg tatactacgt
     5881 tgtttttgct tctattaagc gcgcttctct ttagttacaa aatcttgctt caactggaac
     5941 aaaaagaagc ttcttggatt actgcatttt tcttgttttt taatgatttc aaaagcgcgc
     6001 ttttctattt ggcaggcacg ttgatccttc ttgtcgcatt ctggtttgca ggaccaatct
     6061 attttgcttt attaggtttc agtttcttgt tttattttca agtcctgctt tttatccatc
     6121 gtacgcaaga aaaggttctt aaaaaggagg aataacatgt tgcgtttgat tgctaaaatc
     6181 gatgatagta ttatcaacaa gttcgccaag caacggaaaa cggatagtcg aaaaatcaat
     6241 tttttgaaat ttatcagtaa tatctctaat ggtggtttta tttggttgct ccttgctgct
     6301 gttttagtct tgatacctga tactagagaa tatggtctag ccattcttat cggcagtttg
     6361 atctattttt ttgttggttt gctttttatc aaaaaaacga caagacggaa aagaccttat
     6421 gaaaaaaatc gtcggcttgt cctttacaca agtaaaccga agacttcttc ttttccttca
     6481 ggacatacag cgctagcctt tttatcagta gctgcgttgt tcgaattaag tccagttgca
     6541 ggaatattag cctttttatt tgcttgtttg atcgcttatt cgaggatttt tttactttta
     6601 cattatccta ctgacatttt aggaggagct attttaggaa gtgttattgg catcctatgc
     6661 gctgccgtta tctgaattaa ttgtacgaat aaagcatgag tgcgtaaatg cgcatttgtg
     6721 ctttattttt ttgtttcgag gaaagcgtag ttgttta
//
LOCUS       DAHEYX010000147         6731 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHEYX010000147 DAHEYX010000000
VERSION     DAHEYX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.148"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..299
                     /locus_tag="MB219_002069"
     CDS             <1..299
                     /locus_tag="MB219_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427778.1"
                     /translation="DYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPV
                     RTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(396..830)
                     /locus_tag="MB219_002070"
     CDS             complement(396..830)
                     /locus_tag="MB219_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8427779.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1062..1772)
                     /locus_tag="MB219_002071"
     CDS             complement(1062..1772)
                     /locus_tag="MB219_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCC8427780.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1762..2622)
                     /locus_tag="MB219_002072"
     CDS             complement(1762..2622)
                     /locus_tag="MB219_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCC8427781.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2627..3265)
                     /locus_tag="MB219_002073"
     CDS             complement(2627..3265)
                     /locus_tag="MB219_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCC8427782.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3280..3579)
                     /locus_tag="MB219_002074"
     CDS             complement(3280..3579)
                     /locus_tag="MB219_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8427783.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3622..5106)
                     /locus_tag="MB219_002075"
     CDS             complement(3622..5106)
                     /locus_tag="MB219_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCC8427784.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5126..5587)
                     /locus_tag="MB219_002076"
     CDS             complement(5126..5587)
                     /locus_tag="MB219_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8427785.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="MB219_002077"
     CDS             complement(5605..6672)
                     /gene="ulaG"
                     /locus_tag="MB219_002077"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCC8427786.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
BASE COUNT         1966 a         1491 c         1068 g         2206 t
ORIGIN      
        1 tggattatac tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt
       61 tcatgtataa agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc
      121 gtataaagcc agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta
      181 ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata
      241 ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc
      301 tgagcattgg aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact
      361 aagctagaat tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc
      421 ttttttcttc tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat
      481 cctactgatt cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg
      541 actggtccat aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct
      601 tggcgctgat acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca
      661 gtgatccaac gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca
      721 gattgttcaa atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg
      781 ctcttatata attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc
      841 ctttcttata caattctccc tctaataata cagttctttg actatttaat ctatcacata
      901 taactctcct gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat
      961 actttttgat aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat
     1021 caagtgttat cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca
     1081 tactttccat gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata
     1141 ttggggttga tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg
     1201 ttttctactg ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt
     1261 actccaggga cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc
     1321 cctgtatgaa cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga
     1381 accttaccat aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct
     1441 tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa
     1501 gtttgataca aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct
     1561 attatatttc ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg
     1621 acaccgcttg gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc
     1681 caagtcaatt tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc
     1741 ttcattgctg caattttttc ttcacaagac atcatagcca gcctccttca atttagaact
     1801 taagtagtct tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat
     1861 ttcaatcaga aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa
     1921 atcaacacaa ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac
     1981 agggtaagta tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg
     2041 agccggatta ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa
     2101 aggtgaatga atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc
     2161 atccatgatt tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt
     2221 tattccttcg ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta
     2281 tccagctatt tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat
     2341 tagttgtttc gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg
     2401 cgcacttaaa caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc
     2461 agctatcttt tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga
     2521 catctcgata aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat
     2581 atctttgggt aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac
     2641 gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac
     2701 cacgaccagt gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat
     2761 ttaatccacc agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata
     2821 aatctttttc accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta
     2881 cctgccggat accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga
     2941 gttctacttg caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac
     3001 aacaaatgac tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc
     3061 cagcgtctgc acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac
     3121 agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta
     3181 ctacatcacc aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg
     3241 caatttgcaa attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca
     3301 ctttgtttca gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat
     3361 aactgtaact ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa
     3421 aactacatcg tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt
     3481 ggtaattggt acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct
     3541 tgttcctgat ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt
     3601 gttgtctaat cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc
     3661 tacaatcaaa aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa
     3721 accaatgaca actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa
     3781 tggccaagct gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat
     3841 ccatgtagcg aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag
     3901 gcaagcggct ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa
     3961 gaacaacgga atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc
     4021 aatcatcaaa aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga
     4081 accaaagcca aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt
     4141 tgagattcct tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa
     4201 gatcgccaga tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg
     4261 cccttcttgc atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat
     4321 cccaaagaaa aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa
     4381 gaatcctggt aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt
     4441 ttcatcccgc tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg
     4501 ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa
     4561 gttagaagct actgcccaat aacaacctaa aatcagtccc ataaaaatca atacttgtat
     4621 cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac
     4681 ctgaacgttt ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa
     4741 taagatattt aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc
     4801 aaatgtctcc attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat
     4861 ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa
     4921 accaccagag ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat
     4981 gcattcatga agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag
     5041 gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc
     5101 ccccatcttt tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat
     5161 cgatggagtt cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata
     5221 atttttggat attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac
     5281 tagccgtttt ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg
     5341 taaatgaaat agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta
     5401 cttgaggtgc gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca
     5461 catacgtttc atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt
     5521 cacgccaatc cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt
     5581 tcagcaagaa actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat
     5641 cgagaagaca tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg
     5701 atagatgaat ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc
     5761 ctttttgaat ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat
     5821 atcgtagtgg acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc
     5881 aacagaagta actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac
     5941 atcgattttg tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc
     6001 atgataaaga ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc
     6061 ctgaggcatt tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg
     6121 atcaaacgct tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt
     6181 tactgtaatc gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt
     6241 tggcccaatg aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc
     6301 tagatgatcg gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg
     6361 atcgattacg aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg
     6421 catcatctga tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca
     6481 ccatagatca cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc
     6541 cagccaccac atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa
     6601 gtatgtgccc cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac
     6661 agttttactc atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa
     6721 tgtgtatcag c
//
LOCUS       DAHEYX010000148         6541 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHEYX010000148 DAHEYX010000000
VERSION     DAHEYX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.149"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(100..963)
                     /locus_tag="MB219_002078"
     CDS             complement(100..963)
                     /locus_tag="MB219_002078"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCC8427787.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(1105..3678)
                     /gene="mprF"
                     /locus_tag="MB219_002079"
     CDS             complement(1105..3678)
                     /gene="mprF"
                     /locus_tag="MB219_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCC8427788.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3819..4625
                     /gene="proC"
                     /locus_tag="MB219_002080"
     CDS             3819..4625
                     /gene="proC"
                     /locus_tag="MB219_002080"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCC8427789.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4704..6260)
                     /gene="rny"
                     /locus_tag="MB219_002081"
     CDS             complement(4704..6260)
                     /gene="rny"
                     /locus_tag="MB219_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCC8427790.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         1931 a         1443 c         1127 g         2040 t
ORIGIN      
        1 agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata ccattttagg
       61 attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg ttcgtactaa
      121 ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc ttgaataatg
      181 ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat ctgctccgta
      241 cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg ttatatcttc
      301 taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga tggcttcagc
      361 aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa acactgtacg
      421 tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca tagaaggtaa
      481 tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga cacagtaagg
      541 aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac agcccagatt
      601 ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc cgcctaatcc
      661 agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga tattgtattt
      721 attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg gtaaaaatgc
      781 tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata gatgatctgc
      841 caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc gtctatataa
      901 tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca tcctctcacc
      961 cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag tatagcttat
     1021 gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat
     1081 aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg ccaaactcgg
     1141 aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa atccgaaatt
     1201 acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt ttaatttctt
     1261 ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga tatgcatgac
     1321 tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag ctaagcctag
     1381 attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta acagatagtc
     1441 agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg cctgcttctt
     1501 cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga caataccgac
     1561 accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc
     1621 accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt ccggcagttg
     1681 atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa gttgctgact
     1741 cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta ctttcgtgaa
     1801 gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat aaaacgcaag
     1861 ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc gcatcatttt
     1921 aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct gataaccaaa
     1981 aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg acaagcgaag
     2041 ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc
     2101 gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg ccactaaagc
     2161 tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg aaggaaacaa
     2221 aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat
     2281 tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag ctccccaaga
     2341 ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta acagaatcaa
     2401 gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta aaataccaaa
     2461 agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa gtgctctagc
     2521 aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa aattgaatga
     2581 gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga ttgttgatag
     2641 aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat gcgcaacctt
     2701 ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc ctgtatgtgt
     2761 gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc gataatataa
     2821 tagccaaact actgctacat cttgccgaac aggcaattga ctcattccta agatcatcaa
     2881 aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca atgtagcaat
     2941 catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact tgccgatgat
     3001 caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca gaccaagcaa
     3061 gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa aaaagaaaat
     3121 gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa aaatactttc
     3181 tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca aaccagtcag
     3241 cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg tctgctttcc
     3301 ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga gattgttgat
     3361 cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg gttttccttg
     3421 tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga cacccagcag
     3481 tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat
     3541 atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa agactaacac
     3601 ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt gcagcatcaa
     3661 gtatcgttta tttttcacat tttcccattc ttaacttgta tacttttgtg tcatacccca
     3721 atgttatctt atttatttta atttttccac tttagacctg ttctttgctt gcgatgatag
     3781 aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga tttatcggca
     3841 cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca gtctcgaaag
     3901 aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct gctgaaactg
     3961 gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc gttcttttag
     4021 ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat agccagaaag
     4081 tcttattcat ttctattgct tcaggaaaat cattaacaga tttagaaaca gcgcttgaag
     4141 atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg
     4201 tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa gctacgatga
     4261 tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt acattcattg
     4321 gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg ggacggactg
     4381 gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa gcaattatgg
     4441 gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta gaccaagttt
     4501 cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca ggattcatcc
     4561 cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg ttgaataaaa
     4621 aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg
     4681 cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt ttcccgaata
     4741 acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt acgaatatca
     4801 cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat tacccgaact
     4861 tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt cgaaatattt
     4921 tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc accaggtctt
     4981 gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt agcttccaca
     5041 tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta cttggcagct
     5101 aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc tttcccaata
     5161 tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc tgctgctaag
     5221 ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt
     5281 ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg agcaccgact
     5341 tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt acgagatttc
     5401 tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag tttttccaag
     5461 gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac tgcttccggt
     5521 gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat attacgacct
     5581 tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac tgaaacagtc
     5641 gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa gtttttcgct
     5701 ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag agttaactca
     5761 tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc ttttgataaa
     5821 ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac gtctttttct
     5881 cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc aagtgagttc
     5941 tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg aatcaaacga
     6001 ttctcttgag attttaattc taacttactt tctctcagct cactttcaac ttcagaacgg
     6061 tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt ttctgcttct
     6121 ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat ttctttctcg
     6181 tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc gatgatagcg
     6241 aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt tttatcatct
     6301 atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc atatatgtgt
     6361 acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa ctttaaataa
     6421 acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa atgacactag
     6481 tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc tttgtagatg
     6541 a
//
LOCUS       DAHEYX010000149         6495 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHEYX010000149 DAHEYX010000000
VERSION     DAHEYX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.150"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(588..1067)
                     /locus_tag="MB219_002082"
                     /pseudo
     CDS             complement(588..1067)
                     /locus_tag="MB219_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(1226..1927)
                     /locus_tag="MB219_002083"
     CDS             complement(1226..1927)
                     /locus_tag="MB219_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8427791.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(2035..3444)
                     /locus_tag="MB219_002084"
     CDS             complement(2035..3444)
                     /locus_tag="MB219_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCC8427792.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(3492..5357)
                     /locus_tag="MB219_002085"
     CDS             complement(3492..5357)
                     /locus_tag="MB219_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8427793.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(5478..6329)
                     /locus_tag="MB219_002086"
     CDS             complement(5478..6329)
                     /locus_tag="MB219_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8427794.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         1851 a         1462 c         1087 g         2095 t
ORIGIN      
        1 aatacttgtt tcttttatta acgaaaaaat cattttgttc tcgtgttatg tttcactgat
       61 aaatgatgtt tctctaaata aaaaagccga cgaactaaac gatcaaaatt tgatcatttt
      121 tgttcatcag cttctcctaa aaatcttttt gttaacgata aatagctgga tttgtaaaaa
      181 tatcacttga atcgtcactg ctggaagaaa attcagtgat aaccgcacct gttgcgcctg
      241 cctggaacta gtgtttgata ttgggctcga acgtgtactg atctctagca tgaagaataa
      301 tttctttttt tactgtataa taactttctt tcctttttgg aatagtacag gaaagttttt
      361 ttgtttcctt tccttcgaca taaggtatac cagacccttt cgacaacgaa aggttctcat
      421 ttttccttca tcgtcatttc aaggagggtg ccgatgctct ggacagtttc gatcgctcct
      481 aaaccaaaat ctgtatattc gattctttct aactctgatt ctttcatacc attcctgtct
      541 caaggtacat ctattgtact tttttcttgt aatccattct gcctctccta actaattgtc
      601 aaatcatccc aagatatgtc ttcttcctct ttttcttctt ggatgatttc tgcattcgga
      661 tctactggtt ttttcaataa taaaatggtt attcctgtga caaaagaacc agctaacaat
      721 gcaccaatga atgcccatgg tctggtcatc gtagcgactg caaacattcc cgatgccgga
      781 atacggctat ctgcgcccca aaacatgctt aatccgccac ctactgtccc accgactccc
      841 gttgctatga cattgcgtgc caagtcgttc aatacgatag ggaaacatcc ctctgtgatc
      901 tgacagatcc ccataggaaa agcggtcttc aatgtatcga tttcttgacg attgagtata
      961 tttttacgaa tgattttccc taagaaataa gcgatcgtca agccgaatgg ggttaccatc
     1021 gaagctaaaa tcaatacagt gattggttca tagattcctt ctgaaaatcg tgccacaccc
     1081 ccttatccag aataaatggt aatccagaaa cagccgtgta tctgcggtaa taagcctaac
     1141 caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg atgcagggca
     1201 gctttttcac aacctctttt tttatttatg tttcataatc aatatggtaa ggatgcagct
     1261 ccgtatggta gaccgcctcc gaatattcaa tcatttcccc aatttgcgta gacgatttcc
     1321 gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat tccttgttct tcttctgtta
     1381 aaaatactgc cgagaaatca tcagaaaatg agtcaatagt atatcctttt ttcgtcagca
     1441 aacgatacaa agattctttg ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa
     1501 gataatgaac aaaataaata taaggctgat tgttcaaata atataagcgc ttaaaacaaa
     1561 cagcttgctc accgaaaaag gaaaacaaag gatcttcctt ctttatatgg accaattgca
     1621 aatccagcgt ttctttccgg acttcatatc gggagttctc caagatttgc gtaaagcttg
     1681 ccgctttgga taatcgattg taaggacgat tgcttagaac cgtcgtccca cgaccacttt
     1741 tctttgcgac atattcatct gatgccagca gttcgattgc ctttcggaca gtgattttgc
     1801 tgacttgaaa cagttcttct aattcacttt ctgtcggcaa catcgaaccg actggatatt
     1861 ttcctgagaa aatatcttct ttaattttat ctgcgacttc caagtatagt acacgtgtcc
     1921 ttttcataac gtcacccttt gctttactat tactaaaaga atagcacata aaaaataaaa
     1981 aaagaacaca aatcacatgt gagaagtgtg ttctttttca ttcagctgct ttaattacaa
     2041 gtttacgcca tttgaggcaa ttacttcttt ataccaatca aaagattttt tctttgatcg
     2101 attcagtgtc ccatttcctt catcgtctag atccacatag ataaagccgt aacgtttact
     2161 catttctcct gttgatgcac tgacaagatc gatacatccc caaggcgtat agcccatcaa
     2221 atctacaccg tctgctactg cattagccat cgcttcgata tgacgacgta aataatcgat
     2281 ccgataatca tcaataatcg tgccatcctc ttccatatga tcaatggctc caagaccgtt
     2341 ttcaacaatg aataatggtt tctggtaacg attgtataat tcattcaatg cgattctcag
     2401 cccttctgga tcgatctgcc agccccattc acttgcctca agaaacggat tttttactcc
     2461 tcccagcaag ttcccttctg aagttgctgc ttgtggatca gtctcattga ttgccgtaga
     2521 catataataa ctaaatccga tgtagtccac tggatattct tttagcaact ggaagtcttt
     2581 ttccgtttgc tctaattttt ccggatcgac tccatatttt ttatgggtac gcgctgtgta
     2641 tgcagggtat tctccacgta cttggacatc cgtgcagaaa aaattgaatt cttggttttg
     2701 gatcattgtt gctgcctgat tgactggatt ggcatcgatg ctgtaagttg ttgcatagat
     2761 aatcatgcag ccgatttgga tatccgaacg caattcgtgg ccgattttta cggctttgct
     2821 acttgcaacg aattgattat gccatgcttg gaaaatattt tggtactctc ctgctccatt
     2881 gctttttact aatccttgac ttaaagctgg aaagtgaaag gccgaattga tttcattgaa
     2941 agtcatccaa tatttgactt tactgctgta tctctctaat actgtctgag caaatcgttc
     3001 atagaagtcg atcagttttc gatttttcca gccaccgtat tcttttgcca aatgaagagg
     3061 catttcgtaa tgagaaatcg tgatcacagg ttcaatatca tattttaagc actcatcaat
     3121 cagctgatca taaaattcta atcccgcttc gttaggagta ccttcgtctc catttggaaa
     3181 aattcgtgtc caggcaatcg aaaaacgata acatttgaat cccatttccg caaacaaggc
     3241 aagatcttct ttgaaacgat cgtaatgatc gattcctcga tgattcgggt aaatatattt
     3301 ttcttgatca atcgtccagt caaattcttc actgccgata atcgcaaagc gctgcttacc
     3361 tccaggcata gcatctgcaa cggatagtcc ttttccgtct cgatcataag ccccttctaa
     3421 ttgatttggc agcagttgct ccaccccata aaaatttttc tgaaaaacgt gattgcatcc
     3481 tgatttcctc cttattttac aattgccaga acatcttcgt ttgcaataat ctctttttgg
     3541 tctaattcaa gtatatcggc aaatgcatca gtattcgtaa tgacaactgg agtgattacg
     3601 ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa tcaataaatc tcctgctttg
     3661 acgagatccc cttgtttgac taagatctca aagccattcc catttaattc tactgtatcc
     3721 atcccgatat gaatcaaaat ctccgctccg tcagtcgttg tgatccctac agcatgtcca
     3781 gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa ctagttctcc tacttctgga
     3841 tcaatagcga taccttttcc catcacgcca gaagaaaaaa cttggtctgg tacttcactc
     3901 aatggcagta ctttacccgt taaaggactt tttaaggtat gccgtgctga agtaatcggt
     3961 tctcctgcat tcgcatgttt gttttctaat tgattgtctt gtgtttgttc gtcaaatcct
     4021 aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc ctgtagctat tactgctact
     4081 gtcacatttg actctacacc atccaccgta ttaatgaaag tcggaatcgt caataagctg
     4141 accaaactgc tggagaaagc ttttactccg gaaaatccga taatagctcc accaatacct
     4201 ccagaaatac aagcggcaat gaatggtttc ttcaaaggta atgtcacacc atacacagtt
     4261 ggttcagtga ttccaaataa cgaagtcact gtagaagaaa tccctaatgc tcgaagtttc
     4321 gtatcttttg ttcgcaatgc tacagctaaa gcagccccac cttgtgccaa gatggctgga
     4381 agaagcattg gcatcaatac atcaaaccca tattgttcga tattcaaaaa catgatcggt
     4441 acgaatcccc aatgcatgcc gaacatcacg aatacttgcc ataaacttcc catgattaga
     4501 cctgctacga ttggactaaa tccataaatc gagttgaaga gaccgcctaa agcattaccg
     4561 gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg gaaccatcac tactaaaacg
     4621 acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca ctttttttac gaatggctct
     4681 aatttacttt gaacccatac ggctagaatg attggtatga cagaagaagt ataaccagat
     4741 ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt ctcccgcacc agcaactgct
     4801 gtgatagctg gatacacaag tgccatagca attgctactg ctaaaaactg gttggctttg
     4861 aactttttag ctgctgtaaa agcaagcatg acaggtaaga aggtaaagac accatctgct
     4921 gcagcaaaca atatttgata agtaccgcta tcagctgtca gccagcccat caccgttgcc
     4981 aatgataaga atcctttcaa taccccggcg gctgccatcg cccctagaaa aggtgtaaat
     5041 atactagaga taatatcgat cagacgattc aaaatatttt tggactcttc ttttccatcc
     5101 tccgaaactg caagaccttc ctcatccacg atcgattgat aaacatctgc tacattactg
     5161 ccaatcacta cttgatactg accgccactt tgtacgactt ggatcacgtc cgggtcttct
     5221 tttaactttt gcgtatctgc taaagattca tcttttaatc tgaatcgcaa ccgcgtagca
     5281 caatgtacta aacttttgac attcttttgt cctcctaccg cttcccatac acgacgacca
     5341 actgcttgat tatccattgt tttttccttc tttctttttt atttaaaaaa taaaaaccca
     5401 gattgaaaaa aacagtcaca aacattcgtg aacctatttt ttcaatccag gttttgcctg
     5461 ccatacagta acaatcctta tgattggatg ctttttttct cacttactac tctttgaata
     5521 tgaatcgtta aataaatctg ctctgcgatc cccatttcct gtttttgcgt ttgtcgaaga
     5581 tactcattga tccgttcgac acaatgaaac gcttttggat actttccttg aactaatgcg
     5641 tagagaaatt catcttgctc gtcagatcct tcccctttga gataacgctg ggcaaaaaac
     5701 tgcagatgag taatgatacg ctgataattc aaagattctt catcgaacac gatgccgaag
     5761 aatttactga tgatactcag tatatctctt accattgtgg tagcggtcat cgtcacttgc
     5821 atgtcttcgt ttccttgttg gctattcaca aaatgaaagg caataaaacc ggcttcatcc
     5881 tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa gagcctcttt cgctgcttga
     5941 tattctttcg gataaaattt ttttgtttca aataataatg gattaggcag ttggatattt
     6001 tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca atgtcagata aatattaggc
     6061 gaaaattcaa cttttagttt atcttcagcc agttcgacta cttgtttgac ccattcgatc
     6121 tcctctgccg gaatatcagc catcagctgc tggaattggt ttttagcaaa agaatctctt
     6181 aaaacaaatt ctttttggac cgcttctggc gaaatctcgt ccccaatctt tttttgaaaa
     6241 gctagtcctt ttcccataca aacgacttct tccccgaatc catttttcga aatgacgaca
     6301 ttgttattca aaattttatc aattttcatt ttgcttcgcc ctctcaagaa aaataaaaaa
     6361 acctagaatt cgcctactgt ttcttagctg aattctggtt ttgcctgccg tacagtaaca
     6421 atccatttct tattcaaaat catcatacca tcgaattttt atttatgcaa gccttttcat
     6481 ataaaaaata aaaaa
//
LOCUS       DAHEYX010000150         6483 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHEYX010000150 DAHEYX010000000
VERSION     DAHEYX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.151"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..374
                     /locus_tag="MB219_002087"
     CDS             <1..374
                     /locus_tag="MB219_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427795.1"
                     /translation="IIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGLPT
                     QQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLVSQ
                     LESGELTFEDAQQIQAYLEDR"
     gene            395..544
                     /gene="rpmG"
                     /locus_tag="MB219_002088"
     CDS             395..544
                     /gene="rpmG"
                     /locus_tag="MB219_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCC8427796.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            568..1551
                     /locus_tag="MB219_002089"
     CDS             568..1551
                     /locus_tag="MB219_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCC8427797.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            1581..1703
                     /locus_tag="MB219_002090"
     CDS             1581..1703
                     /locus_tag="MB219_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCC8427798.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            1721..3244
                     /locus_tag="MB219_002091"
     CDS             1721..3244
                     /locus_tag="MB219_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCC8427799.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            3268..3507
                     /locus_tag="MB219_002092"
     CDS             3268..3507
                     /locus_tag="MB219_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427800.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3700..4335)
                     /locus_tag="MB219_002093"
     CDS             complement(3700..4335)
                     /locus_tag="MB219_002093"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCC8427801.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4356..4574)
                     /locus_tag="MB219_002094"
     CDS             complement(4356..4574)
                     /locus_tag="MB219_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427802.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            4700..5904
                     /locus_tag="MB219_002095"
                     /pseudo
     CDS             4700..5904
                     /locus_tag="MB219_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            5899..6306
                     /locus_tag="MB219_002096"
     CDS             5899..6306
                     /locus_tag="MB219_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCC8427803.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2275 a          975 c         1263 g         1970 t
ORIGIN      
        1 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
       61 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
      121 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
      181 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
      241 agttatttca atgtattgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
      301 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
      361 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
      421 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
      481 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttca aagaagtgaa
      541 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
      601 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
      661 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
      721 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
      781 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
      841 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
      901 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
      961 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcata
     1021 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
     1081 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
     1141 acgattcaaa aaaaggattt ttataatttg gaaaaattca atcatccctt agtttttgaa
     1201 agtgattcat cagaaaatca ctataatggc aaacactatc atcaacatca tcaagaacat
     1261 gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt tttattccaa
     1321 ttaattgacc ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt
     1381 gctttgcaag atcaagattt tatagttgcc atacaagggg taaacgaaca ggttaatttt
     1441 caatacacca ctcgacaaaa agaccaaacc actattattc taattggaaa aatgttggat
     1501 gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta
     1561 ttgattaggg gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta
     1621 aaagcccgaa tatcaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacaaa
     1681 ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg
     1741 acttattggg atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa
     1801 agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga
     1861 tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc
     1921 agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt
     1981 ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa
     2041 gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga
     2101 agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg
     2161 ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt
     2221 gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa
     2281 acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac
     2341 acaacatgca gcctttggat atttagcttt ggattatgga ttgatacaag ttcctattgc
     2401 tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt
     2461 taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata aaatcgcaaa
     2521 aactttggcc aatgaagctg ggataaaatt ggaagtattg aatcctcttg aaagtcttac
     2581 aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc
     2641 gttagagaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga
     2701 tgaaaagaca gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag
     2761 tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga
     2821 agttttcgat tacaaagcaa aactcaataa agacatgaca gctgccgagt acaaagatta
     2881 ttatactact ggctataaga cagatattga tactatcaac attaaagaca acaccattga
     2941 ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt
     3001 gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc
     3061 tggtcgtttt aaatatattc aatttagcga tcatggaatt gcaccaagta aggcagagca
     3121 tttccatatt ttctttggtg gagaaagtca agaaaaactt tataatgaaa tgcataattg
     3181 gcctactttc tatccagctt cattatctga gcatgagatt gctcaagaaa tgatggccca
     3241 ttaaaatcca atctttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc
     3301 aagaatattg atgcttatcg gcgttatcaa tttgcaaagc aaatgagatt aaatgggagt
     3361 acattagctg atattgctgc aactttacat gttagtattg acacagttca tagagtatta
     3421 aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca
     3481 aaacggcata atagcttggc caattaattg ataataccag agtagatcat ttttccctta
     3541 tttggatttg atctgctttt tattatttct tgaattgtca aatcaagcgc aacactttat
     3601 caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt
     3661 tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca
     3721 agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg
     3781 atctggaaaa taaaagggca tattattcaa tacttctgta actgcgaaat gattggagaa
     3841 ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag
     3901 ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa
     3961 aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccactag ttttgccagc
     4021 tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt ttggatggcc
     4081 atggatcgaa tgagtaaggc aaatcttccc acgagactcg acatgatttt ttgtatgtct
     4141 tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc
     4201 tgtatcgaaa agatcattgc aaattgcacg gtagatcgtg ataaaactaa ttgaaaagtc
     4261 catatgttca tgcttcaaac gattcgagat tttctctggc gaccactgaa aatcgacaaa
     4321 taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt
     4381 ttttcttcga taagcataat ttttttccgc tttggaaggt gattagtttt tttcttgttt
     4441 cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta
     4501 atagaaaatc cttcttcgtg aagcagaaag attctttctc gtttgagtat agtaagatga
     4561 caatactggc tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg
     4621 gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa
     4681 agaggttccc tatgaaaaaa tacctattac aatcttaact ggctatcttg gttcaggaaa
     4741 aacgatactt ctaaatcata ttctgtacgg tgatcacgga aaggaaattg ctgtcattgt
     4801 aaatgacata ggggaagtaa atgttgatgg ggtattagtc gacaagaaag gttttaaaag
     4861 aactggagaa aaacttgttt cgatgccgaa tggatgcatt tgttgtacat tacgcgaaga
     4921 tttgattgtt gaactagatc aattagtgca attttctgac ttgaatcaaa ttgttattga
     4981 agcttcagga atcagcaaac caattccaat tattcaagca attatattag ctaaaaaccc
     5041 tttagacatt gatttgaccc aacgattaga gattggtgcc attattatag tagtggatgt
     5101 aaaacgtttt tgggataatt tcgaaacggg tgaaaagatt caggaaagaa aagctgaagg
     5161 taatgtagtt gaagaagaac aggatattcg tggcttgtta attgatcaat tcgagtgttg
     5221 taacatatta cttttaaata atagtgactt agtgtcaacc aagacgattt acaaaattaa
     5281 cggtctaatt gctaagttac agtccaatgc caaaataatt aggacaatta aaggtgaagt
     5341 agacttatcg aaaattctaa atacaaatct ttttaatata gatgagactt cctattcaat
     5401 gggctggttc aaagagttag agcttggcta tcaaaatcat attcctgaaa cagaagaata
     5461 tggtatctca agttttatct atcgaaatcg gttactcctt gacgcacaaa aatttgccca
     5521 tcttacggat aaaaattttc ctggcaatat tacaagagca aaagggtttc tttggtttgc
     5581 ccaacatagt gatttttcga cgatctttga aacatcgggt agccataaaa gtattgaatc
     5641 attaaattat tgggtagcta gtttacctaa aattgagtgt caaatgatta tacaaaaaaa
     5701 cccgaattga aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt
     5761 attggtatga agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat
     5821 tctcttaatg cagataattt ccgaaaaatt tcaccatttc catggaaaaa ttctaatatg
     5881 tttagccaga tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc
     5941 ataattattg gcattttgat tattccctct tttattccct tatttgtatt ctctatagaa
     6001 aaggagaatg aaacaactga agatgctatc aaatatgcca tcgttcaaat tgatggcaag
     6061 gaaatggatc gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg
     6121 aagggacaat ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat
     6181 agtcctgatc aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt
     6241 tgtttacccc ataagttagt tattagtatt gagaaaaaag aaagcaaaga ttactacatt
     6301 tactaatctg gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat
     6361 agcaggggct ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac
     6421 aggcacaagt atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt
     6481 att
//
LOCUS       DAHEYX010000151         6406 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHEYX010000151 DAHEYX010000000
VERSION     DAHEYX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.152"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            359..640
                     /locus_tag="MB219_002097"
     CDS             359..640
                     /locus_tag="MB219_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427804.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            642..1058
                     /locus_tag="MB219_002098"
     CDS             642..1058
                     /locus_tag="MB219_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427805.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1680..2834
                     /locus_tag="MB219_002099"
     CDS             1680..2834
                     /locus_tag="MB219_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCC8427806.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2828..3250
                     /locus_tag="MB219_002100"
     CDS             2828..3250
                     /locus_tag="MB219_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427807.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4420..5388
                     /locus_tag="MB219_002101"
     CDS             4420..5388
                     /locus_tag="MB219_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCC8427808.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5405..5929
                     /locus_tag="MB219_002102"
     CDS             5405..5929
                     /locus_tag="MB219_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCC8427809.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            6105..>6406
                     /gene="tnpA"
                     /locus_tag="MB219_002103"
     CDS             6105..>6406
                     /gene="tnpA"
                     /locus_tag="MB219_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCC8427810.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT         2414 a         1095 c         1046 g         1851 t
ORIGIN      
        1 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
       61 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
      121 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
      181 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
      241 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
      301 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
      361 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
      421 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
      481 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
      541 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
      601 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
      661 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
      721 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
      781 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
      841 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
      901 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
      961 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     1021 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     1081 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     1141 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttgaggcgct
     1201 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     1261 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     1321 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     1381 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     1441 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     1501 gtgagcgtct ctatccattg gtagaggggc tacttggcat agtgtacatt atgttaacta
     1561 acgctataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     1621 ttatcgcata ttgtatattt taattatagg tgtataccac ttaaatttaa cacaaaggcg
     1681 tgattatttt gaaacccaaa aagaaaattt atctaggtct cctcttttcc ttgatgattg
     1741 gaatttttag tataggaatt acaaatacct atgcttcaga aaattctaat ttcaatatca
     1801 actcccaacc aattgaagaa caagcaatta attttgcgaa aactgatttt gaacactcca
     1861 taacttcaat tcttttaatg gaaaataaca ccgaaactga tattaactat actcttggtt
     1921 cgccatttaa aatgctcgat ggagaaaata cggcaaatag ttctattttc tttccagtat
     1981 tttcagacgc tgataatacg attaaatata tttataccgt caaaaaaaat acaaatggag
     2041 aatattcagg aagtatttca caatttttag caaaagaact tcaaaatcta gtagatagta
     2101 atataacaga tacaacctta ttttttcaag ataatagcat ttattattcc gataatggac
     2161 aaattaaact actttggaaa tcacctttaa gtacagaaaa aaatggtgtt tcttccttta
     2221 cagaaaaaaa tgatcttata tccgtaaata taaaaaataa aattaaaaat ttctctaaaa
     2281 aacaaaattt tcctgcggtt aaacgtgtaa aaagtgtctc ctccggagat aactttatta
     2341 tgattaattg gcgtatagca gaaacccaaa caagttttcc gtggtgtgct gcttacgtag
     2401 aagctgctat cttatgtaac aaaacagatg tacgtgctac ttcagctaga gccattatgc
     2461 attataccta tcctaattta aacaacagtc aaattgaatc aaaaagtctt aatcaagatc
     2521 aaataataaa atatgcaaat tactgtggtg tttatcctaa aaaagtagca cgaatactaa
     2581 acctttcaga ggtccaaaca caactaagaa aaggaaatgc catttatatg ggaactgctg
     2641 gttatggaaa ttatagtaac agccgtcatg cacttgcatt atttgggtgg gtcaatcaag
     2701 gtggttatca aacttactat gtgtggaatc cttggtataa ctacccaact gtaataagtg
     2761 caaatgcagg accagttaca attcctgtac ctggaggtgg ttttacttgg gacaatacca
     2821 ttacaaattg gtaaataaac ccattgcttt agttatgggt ctagtgttag ttcttttctt
     2881 aactagttgt tcaaaaaaac aagaaataca aaagactgat ctgattcctt ttctaggaac
     2941 ttggcaaact aaagatcatt cagaacgtct agtaatcact aatttaaata cttcaaaaaa
     3001 tatagaacaa tataccatct tagtaaaaga gaacaatcat aaaattcaaa tcattgataa
     3061 aaatttaaat ataaatgaac aagttttcat ttacaaagca acaaataatt tactaacact
     3121 gcgctctaat aaaaaacaat tagattacac ttttagtttg gattctccaa aaaagctcgt
     3181 atttattttg acaacgaatg atgggacaga agggacagct aaaccaattg aattttttaa
     3241 agctgattga tgaatagttg tttgatatta caaaacaata atttcaccga aggtgaaaca
     3301 agcgaaactt tacaaaaagt ggctagcatt tgctagcctt ttttgtattg tggagcgtgg
     3361 gttttgaggg ggattcaagg gggagcttgc gcccctttga aaagcggtta tttgcaccaa
     3421 atacgtaggt atttggctag caaatataga gcttgccaaa cactttagtg agttttgtag
     3481 aaagtacctt taaaggtact ttcacattac gaacgatacg tccgtaggac gtactgaaag
     3541 ggtgggattt atgagcgaac taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata
     3601 tgctaataat cgagcgaaat gaacactttt tcaagtgagc gaatccgcca tgacaaagct
     3661 gaagcagttt atttcagctt tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca
     3721 cgagcaaata tctttatcaa gctctaattt gcgttttaag acgtttgaaa cgtctaagta
     3781 tataaatata taagagtagg tttaagagtg cttaaatcga attttagagg gcattcagac
     3841 aactttaggg ttgttagggc ttgccctgac cgcctgtaag gcgctttaag aacaagataa
     3901 gggatttata ctaagactgt cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga
     3961 gttccttatg aacattttta gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag
     4021 cagagcgatc acaccttttt aagcgactgt agggagtaag attcccagtt gagaaaaatg
     4081 cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc gggaaatttt gtccccctct
     4141 tttataatct ctttttaaac ctctttttaa acctctttac cgtcggctat atccttactc
     4201 tcccaaggga atgacgcttt tatctacatc gttttgtctg tttatctaca tcgttttgtc
     4261 tgtttatcta catcgttttg tctgtttatc tacatcgttt tgtctgtttg tacattgaat
     4321 tagtacatcg acgatgttaa tataagatgt agataaacag gcattttgta tttcataacg
     4381 tgtatctctt gtgcgagtaa gaaaggagct attttaaata tgtctaatga aatcgtaaag
     4441 tatcatcatg aattaaatac aattccgttg cgaaaattta caccagtcga aatgaatttg
     4501 ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata aaaaagtaca atttacattc
     4561 gagcaattaa aagatttaag caattataaa gcaactgcaa atgttcgatt catagatgat
     4621 ttagaaacaa cgtatgacaa attaatggat ttacgttttg gtagaagaag tgctgacggt
     4681 ttgcaacgtg aacgttttgt tttatttaat caatttaaaa tcgacggaaa atctgatatt
     4741 ccttttgctg aaatacaagt tcatgaaaaa gccttaccct tacttaataa tcttgaagaa
     4801 tgggtgcgct attctctcca acaattcaat gaattagaaa gcagttattc aaaaacaatg
     4861 tttcgattac taaaacaata cagaaccaaa ggtttcgctt atttttcaaa agaagacttt
     4921 catgaattac ttgatattcc taaaagttac aaccaaggta cgattgatca acgtgtgttc
     4981 aatccaatac gacaagaact aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac
     5041 ggaaaaggac ggggaaagcc tgtcattggc tatcaattta catttaaacc tgagataaaa
     5101 aaatcagacg actttaacaa acatgcccca acaaaagaag aacaaaacaa agcattagaa
     5161 ctagctaacc aacaagttca atctgaaaaa gcacctactt cattacaaga aaaaatcaaa
     5221 gaacaaaagc gaaaattgga acttttacaa cagttagaaa agttagaaag agaacaaaat
     5281 acccaaaata aagaaaataa tgcacaaaaa tcagagataa attccgatat tccttcagaa
     5341 ctaaaaaaag acttattaga caatcttcaa aacttattca aagattgagg ggtttttaga
     5401 ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc
     5461 aattaacaaa aaagtttctg acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa
     5521 gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca atgtttttta acgaaaacga
     5581 aaactcaaat caacaactag ttggcgaaaa aacgaaaacc gaaaaccaca aaaacgccaa
     5641 ccaagttccc gaattagaag tcctttctct cctagaacaa cagttaaatc aaaaagatat
     5701 gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa aaattactcg accaccaaca
     5761 acaactcacc ctgcaagcca accaacaaat cgagaaactg caggaacaac tacagttgac
     5821 ttatacggaa gaatcttcgg aaaataaatc aacgactctt tctgaagaaa cagaatcgat
     5881 agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc tggcaataaa accagcaatt
     5941 taacagaatt aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccgttcggta
     6001 tgagggttac cgttggtaac tcgaagaccg acaactaagg cgtattgcct ttttattttg
     6061 tcccatgtta caatcagtct atacaagaaa tgagttgata taacatggaa ttagatacaa
     6121 ataatcattc agtatttctt ctttattacc accttgtttt agtaacgaaa tatcgtagac
     6181 aagtgattga tgatgaaata tctgattatg ccaaaactac ttttgaaaga atttcagaat
     6241 cgtatcacat tactttagtg gagtggaatc atgataaaga ccacgttcat attatgttca
     6301 aagctcaacc taaaacagaa ttgacaaaat tcatcaatgc ctataaaagt gccagctcaa
     6361 gattgataaa acgtgacttt ccaagagtca aacaatttct ttggaa
//
LOCUS       DAHEYX010000152         6360 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHEYX010000152 DAHEYX010000000
VERSION     DAHEYX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.153"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(309..3458)
                     /locus_tag="MB219_002104"
     CDS             complement(309..3458)
                     /locus_tag="MB219_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCC8427811.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3471..4670)
                     /locus_tag="MB219_002105"
     CDS             complement(3471..4670)
                     /locus_tag="MB219_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCC8427812.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(4660..6255)
                     /locus_tag="MB219_002106"
     CDS             complement(4660..6255)
                     /locus_tag="MB219_002106"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCC8427813.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1759 a         1325 c         1043 g         2233 t
ORIGIN      
        1 gtcattacag caaaaaatca ctttatctcc gtttcatttc agatacattt cacttaagcg
       61 gattccaaaa aaacaaaaga aaaaggaacc cctagttagc ctatgctttt tattggttcc
      121 tctcaaaaca cggtcatata acaacggggt ggggtaaatg aatgaaaaag gtggaggaaa
      181 cattattcaa tagctgtctt aaatacttcg ttataaaatg tgaacaaata aaagacatct
      241 atccctattt tctacacttc cactttacta ccatctttga atcgtacaat tttcttaact
      301 cttctcgttt acctcaattc attttcaagt ggaatcagct catcatctaa cattttacgc
      361 catgctcgct tcatttcttg tgggtattta aatggcttcg cttctggatg gttggcttta
      421 tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg atcttcacct
      481 ggcatatatt gcagattaga agcatgtaat tctttctcag aaacaaacca ctcattggaa
      541 aagacatgaa tcaaatcatc actcgcttgt gtaaaatagc gacgtttaat gacaaagata
      601 tcttcattac tatctaaagt ccccacatca aacgcattta aaatttgtcg gtaaacttct
      661 ttgactcctt ctgttttatt caatttagcc aaagcttttt caatttcttc tcgtgcatct
      721 gaactattcg ccgcataagt tcctagtaat tgatcaatat aagtagaatc aatatcatac
      781 aacttcgtgg tattttcact ataaaattcg atcgtggaaa aatcttctcc ctctttttct
      841 ggttcttcct ctattaatga acccttcacg gtctcgtaca cgccaatttg ttcttccaaa
      901 agttgaattt gattgttcaa ggttgaagat ttcaccatgt catcattgta atcgtcataa
      961 gtcactaacg attcataagc acgattcaat tcttggaatg ctttgacgta ctcgatacgg
     1021 atttcaagtg gcgtatgttc atcaatggat tccatggatg aaacctcttt taacttacca
     1081 tatgcttgct tgaatctttt tttggattcc tgataacttg gataaatcaa acccgattct
     1141 tcttttgctt ctgaatacag ttttgtcgca tcctcgacat tcttctccat tgtggctggc
     1201 tttcggaaag taacaattag tccttttgtt ttttctggat acgtacggtt cgtacgtgaa
     1261 aaagcttgga tcaatcctgc atactctaga ttacgatcca caaatagggt ttgaatcgta
     1321 ggtgcgtcaa aacctgtcaa tagacgatcc actacgatca ctaaatcaat ttgtttccca
     1381 aattgtttga attccgctct ttttcgtgcc aaacgattat tgatgtctcc gttataacgc
     1441 tcaatgtctg ctaatgacca ggaagtatca taatactgat tatattcctc aataattttt
     1501 tgcatttctt cttgttgggc acttgaatct cggctatttt cttctaatga ataggtaata
     1561 gcgattcgtg gaaaatcagg atcctccatc gttcgtcctt cacgaatgag ctgattggga
     1621 aactcttttg ttaaccaatc aggctctttc gtcatctctt tgatggcccg ataatacttt
     1681 ttggccatgt caatggaact cgtggttaag attgcagatt tcgttggcct gccatttcga
     1741 aaatcaaatt ttgtataggc attatctgga cggaacattt tatgaatgac tttttggata
     1801 tgttcatctg cttcaaaaac agctgggtcc aaataacttt cttttttgac aggttccatt
     1861 tgatcaatca tacgattgat ctgttctgat gaatactctg catatgtttc tacttctttt
     1921 aatttccgat agattttatt ctctagtgaa gtcggctcca cggtgttttc atgctctact
     1981 tgaaatccta agacagagcc atcttctagc gcattcttaa tggtataggt atgcaagacc
     2041 tctccatact gatcatgggt tgtacgtgcc aattgccctt tggcttgttt ccgattctca
     2101 ggaaaaatcg gtgtacctgt aaagccaaac caagtggatt tcggaaacat ttttttgatt
     2161 tcttccatat tttcagcact taacgctcga tgacactcat ctactacaaa cacgatatgt
     2221 tggcccatta atttttggaa gcgattggtt ccttttttct cttcttgttt ctttgcatat
     2281 ttcaacgccg catctagttt ttgacgggtt gtaatgatta caacatttgc attggcatct
     2341 gccaacaggg tttcacttaa ttctttggca cttcccgttc caacgatcaa gctattcgct
     2401 ttggcgttcc cggaagaaat cccagtatta aattccgaag caaattttgt aaactctgtg
     2461 gtggtttgat tatctaaatc tttgcggtcc actaacatga tcgttcgatc gatccccgat
     2521 tttcgtgcta aaagcttagt agatacaaaa ctagtcaacg tctttcctga accagtcgca
     2581 tgccacacat accctgattg atgtttattt gcggcaataa acaaggcttg aatcgcatgt
     2641 acttgataag gatgtaacac catgagggtt ttattatctt gatcttcact gacgattgta
     2701 taatccgcaa tcaaacgatg agcatccggg atatttaaca cttgtttaca aaactcatat
     2761 aagttttcta ctttttcatt gtctttcgtt cgccaactaa ataaaaactt agggtgcaag
     2821 tctttcggta aggcatttgc aaaataccga gtggtttgtt cattggaaac cacaaacaac
     2881 tgtaaggtcg aaaagatatt gttccgaaac ataccttctt ctgcatattt tttgatttgg
     2941 ttaaatgcct gataaacacc atcttttgca gttacttgtt tcagctcgat ttggacaatc
     3001 ggtaatccat tgattaataa ggtgacgtca aaacgtcgat cccgtgcttc tatgttactt
     3061 ccacgttttg caatttgatg gaccacttca taagtagaga ttcctccacc aatgtcttga
     3121 ttggaataaa ggataagtga gacagaaccc aattgcgaat cttcccgttc aatcgtgatc
     3181 cgagccatcc cattttcacc ttttaaccat ttcgcagcat caaatggggt tttcgttcgc
     3241 agcagtaatt ccgttttgat cgtttcaaat tctttatcgg tcaaaggaaa atcattcagt
     3301 tctgcttgat tatttgagat aattttttgg cgtaaattga cccaaaggtc ttcttctgat
     3361 tttagatccg gacgatacgt ccactgatta tgcccttctc caagtacacg aatcaactgt
     3421 tcttcgactt ctgcttcctc acggtgacta attttcattc ttcttccctc ctagacaaac
     3481 atcttttgta agaagctttt tttcgtttct tttagtaaat ctaacttacg ttgatgaaga
     3541 gcgatcgtat catctagctg tttgaagaaa gtaccgattt tttgttgttc atcctttaca
     3601 ggaaccttaa cactgatatt ctcaatatca gaactgttat aagaaggcaa tgcccctact
     3661 tgggagtatc ttggcaaatt tatagtttca aacaatattt taccaaaata gatatcccaa
     3721 gtattattaa ataaataagc catggtattg ttatctatta agaaagggct tgtgaccaaa
     3781 cgttttctat taagcattat tgctgcgcca actttagcaa atataatcgc tggcacatct
     3841 tttattggct tccatttttt acgctcaatt tgctcattag atacataatg atttgcattt
     3901 ctcatttcat actcattacc aatgttattc atgtcggaaa ctttaaaaaa tggagtacct
     3961 tcagaaccac cttgctcggc atcaggaaat ccaattcccg attgagtttt acctaattct
     4021 cccaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggcactttt
     4081 gctccgtttt cagggaacat tttttgtaag aatccttttt tcgtttcctt cagcaaatct
     4141 aacttacgtt gatgaagagc gatagtgtcg tcgagttttt tgaagaatat gccgattttt
     4201 tgttgttctt ttaaatcatt gggaatatag aatactgtat ctgaaacttt tttccagtct
     4261 gaacgaggca tttttgttcc agatgtatca tttgcagcct tttgatatct atcagcttga
     4321 ataagagaat aaataaagtc cgagtcgctc ttcttagctc taaaaaccca gaaatcccca
     4381 atcgctacgc ctgtaaaagt tgccttgagc caatttttta aatatggacg taactttcca
     4441 tatagaatat catttggtaa aaattcaatc ccttttctat tatcaaattt ctggctcaca
     4501 tctttattca gtctaccttc tcctgccacg atatcttcaa attcaacttt tggaagtcta
     4561 ctggagtttg tacttttatt aagtctaaca actaaatcaa ttaacttacg ctcttcccaa
     4621 tcttccgtaa atcctgggaa acggatttct ggtcgtgtat cattgctcat gtttgaacac
     4681 ctccagcgca ccttgtaacc acatttcgtt ttcaggtgtt gtttgcaggg aagagatagc
     4741 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4801 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tctacatagc gtgggatatt
     4861 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4921 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg atagtttatt
     4981 ttgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     5041 acgattcttc ttcaacacaa tcaccgtcgt tggaatcgat gtcccaaaga acaagttcgc
     5101 aggcataccg atcacagcat aaatactgcc atcttctagt aatttttgac ggatgacccc
     5161 ttctgcagcg ccacggaata aaactccgtg tggtaagacg atggccatgg tacctgtttc
     5221 cttcaaatga tagaacccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5281 tttcccataa cgattaaacc gagaatcatc taagaaagtc gtatctgctg accaattagc
     5341 cgaataaggt gggttcatta ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5401 cttattcaac gtatccccat tgcggagatt catttcttcg gcgtcgacac catgtaaaat
     5461 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5521 gttatctggg tgtgttaagt agttacgtac gtttaacatc aatgaacctg aacccatcgt
     5581 tggatcaaaa acactaaaga atcgtcgttc cttttgatcg agcgttacga tttgcgccat
     5641 catatcagaa accatatgag gtgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5701 aaattgactg atcagaaact catacgcatc cccgatcacg tccccatcgt gttctaaaac
     5761 atctacatca ttcaatttct tgatgacttc ggtaatcgtc acatttcgtt gttgctcatc
     5821 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5881 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5941 tgcttgatcc gctaagacgt tgaaaagata ctctggtgca attgcatatc ccaaaatatc
     6001 aacgattgtg gcaatcagat cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     6061 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6121 ttgttccaac aatcgatcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6181 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6241 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6301 tattttagca tacccccttt tataaaaagc aaaaaaaaaa gaaagaaact tattcttgct
//
LOCUS       DAHEYX010000153         6227 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHEYX010000153 DAHEYX010000000
VERSION     DAHEYX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.154"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(82..270)
                     /locus_tag="MB219_002107"
     CDS             complement(82..270)
                     /locus_tag="MB219_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCC8427814.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            540..902
                     /locus_tag="MB219_002108"
     CDS             540..902
                     /locus_tag="MB219_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCC8427815.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            954..2636
                     /locus_tag="MB219_002109"
     CDS             954..2636
                     /locus_tag="MB219_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCC8427816.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2753..4789
                     /gene="recG"
                     /locus_tag="MB219_002110"
     CDS             2753..4789
                     /gene="recG"
                     /locus_tag="MB219_002110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCC8427817.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4838..5839
                     /gene="plsX"
                     /locus_tag="MB219_002111"
     CDS             4838..5839
                     /gene="plsX"
                     /locus_tag="MB219_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCC8427818.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5961..6203
                     /gene="acpP"
                     /locus_tag="MB219_002112"
     CDS             5961..6203
                     /gene="acpP"
                     /locus_tag="MB219_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCC8427819.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2077 a         1084 c         1422 g         1644 t
ORIGIN      
        1 taggaatcaa aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt tcactatctg
       61 aacggtccaa gtatcattca attaaacgcg ttcaactttt cctgatttca aagcacgagt
      121 tgacacccaa acttttttag gttttccgtc gaccataacg cgaacttttt gtaagttagg
      181 tttaacagta cgttttgttg agttcatagc gtgtgaacgg ttgtttccac tttttgtctt
      241 gcgacctgta aagtaacaaa cttttgccat ggtaatttcc tcctttgcct tgatttggag
      301 caatagaata attcagctca taccagacta acatatcata tagaatggga atttgcaagt
      361 acattttcat agatacaatt gacaattgag aagaataatt gtaaattaaa caaggttata
      421 cctgaaattt tgcaaggttt ttgagtactt tgggtaactg ccttttattt caagttgcgc
      481 tatgataaaa tatgatagaa gaaaaatggg agttttttac ccgttaagga ggctcattca
      541 tggctgtaaa aattcaaacg tcagctggaa ccattgaaat taccaatgat gtaattgcaa
      601 cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc agcaaaaatc
      661 aaattaaaga taatattact gagattttgc gtaaagaaaa ttactcaaga ggagtagttg
      721 tccgccaaga agataatggt attgctgtag atgtttatac gattatcagc tatggtacga
      781 aaatctctga agtttcccgc aacgtccaag aaaaagtgaa atacaatcta gaggcattat
      841 taggcgtaac tgccaattca gttaatgtct ttgtacaagg tgttcgtgta ctgccagact
      901 aggcaggaga ctggctggta actcagcagt cgatctgaag gaggattatc taggtgagtg
      961 taacagaaat aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg aatcgtctgc
     1021 aagtaaatgc agaatacgtc aattcattaa atgtattccc tgtgccagat ggcgatacgg
     1081 gaacaaatat gaatctatct atgactagtg gagctaaggc agtagtagat tcaacctcag
     1141 aaaaagtagg agaattagca gcaatgcttt caaaaggatt gctgatgggt gcgcgcggaa
     1201 attccggcgt tattttgtct caattgtttc gtggattttc taagcaaatt ccagatgttg
     1261 tctcattaaa tgcttctgat cttgctgcag ccttcactca tggagttgaa actgcgtata
     1321 aagcagtaat gaaaccagta gaaggaacga ttttgacagt agctagagaa gcagcgaaag
     1381 ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga agtaatgaca gctgttgtaa
     1441 aaggagcaaa gcgtgcttta gctaaaactc cagacttgct tccagtccta aaagaagtcg
     1501 gtgtcgtaga cagtggaggg caaggactgc tgtttgttta tgagggcttt ttaagtgcat
     1561 tgaatggaga attccaagcg gatgaaacat atgaaccttc tccagcagaa atggatgata
     1621 tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa gatatccatt
     1681 ttggttactg tacagaaatc atggtaaaaa tcggagaagg acctacagtc gatagtacat
     1741 tcgattacga agaattccgt aactatttag atggtatagg agattcttta cttgtggtca
     1801 atgatgacga gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa gtcttgaatt
     1861 atggacaaaa attcggttca ttagtcaaaa tcaaagtcga caacatgcgc ttgcagcatg
     1921 agacgatttt ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa ataccacgta
     1981 tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg tttgaaagtt
     2041 taggcgctaa ttatgttatc agtggtggac aaacaatgaa tccaagtacg gaagacattg
     2101 ttaaagcgat tcaagaagta aacgcagata aagtgattgt cttgccaaac aataagaaca
     2161 tcttcatggc agctgatcaa gcagcagaag tagcagatat cccagtagca gttgttcctt
     2221 caagaacgat ttcccaagga ttgacagcta tgctttcatt caatgatcaa gcttctttag
     2281 aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt caagtcactc
     2341 acgcgatcag agatacctca attgacggtg tagagatcaa agaaggagac tttttgggca
     2401 tgatcgatgg aaaaatcctg atctctaacc cagatatcac agcaacagcg ttaagtacac
     2461 tagaacaaat gatcaatgag gacacagaga tcgtaacgat cttggcaggt gatgaaggaa
     2521 cagttgaaca agcaaatgaa tttgctgaga aactaacaga aaaacatcct gatttggaaa
     2581 tcgagatcca ccaaggagat cagcctgtct atccttatct attttctgct gaataatttc
     2641 tgtgtcagaa tatctttcta acacagtatt cattcataaa aggccttcgg ttagtgatca
     2701 ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat ttatgcgttc
     2761 tttagatgat ccaatcacta tgctggcagg agtcggagaa aaaagagcag ctagtcttgc
     2821 cagcttgggc atccaaacga ttgaagactt actgacttat tatccttttc gctacgaaga
     2881 tattcaggaa agaaatctaa acgagatcca ggatcaagaa aaggttactt tgaaaggaat
     2941 cgtagtctcg cctcctgtga tgaatcgatt tggttataaa aaaagccgat tgcaatttcg
     3001 tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt aatcaacctt atctaaaaga
     3061 aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa agcgtaaagc
     3121 cttgaacggg atgaagattt taggttctca aacaatggat gacttttcgc cgatatatca
     3181 cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta atcaaaaaag ggtttcaaga
     3241 atacggcaca ttgattgaag aaaatctgcc aaacaacctt gtggagaaat atcgactgct
     3301 tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag aaaaccatca
     3361 agccaaacga cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc ttcaaggatt
     3421 gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc ctttatgatg tccaaaaatt
     3481 gaaacaattt actcaaaagc taccttttga actgacaaat gcacaaaaac gtgtaacgaa
     3541 tgaaatctgt cgggatcttc gcagtccgca gcatatgcag cgacttttgc agggagatgt
     3601 aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta cagcaggttt
     3661 ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa caacatatgg aaagcttgca
     3721 acagctgttt gatcccaatg aagtaaccat cgcattgatt acggggtcaa cgaaagcaaa
     3781 agaacgacgt gagctccttg aaaaactaga gcaaggggaa attaacgtcg tgatcggcac
     3841 acatgcattg atccaagatg gagtcgaatt tcagaattta ggtctagtta ttacggatga
     3901 gcagcaccgt tttggcgtga accagcggaa agtacttcgt gaaaaaggct ggcgaccaga
     3961 tgtcttattt atgacagcga ctccgattcc tagaacatta gctattacag catatggtga
     4021 gatggatgta tctatcattg atgaacttcc agctggacgt atcccgatcg aaacacgctg
     4081 ggtaagaaca cctcaattgg acagtgtact ggattggacg aaaaaagaac tggcaagagg
     4141 acatcaaatg tatgtcatct gtccgctgat tgaagaatca gaagcattag atgtaaaaaa
     4201 tgcagtggaa atctatgaaa aacttcgtga actatttgca ccaaaatatg aagttggctt
     4261 gttacatggt aaaatgaaga atcaggaaaa agaagtaatc atggaagcct tcaaagacaa
     4321 ccagatgcag atattagttt cgactactgt aattgaagta ggggtaaacg tgccgaacgc
     4381 gaccgtcatg ctgatcatgg atgcggatcg atttggattg gcacagctgc accaacttcg
     4441 tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta atcctaaaaa
     4501 tgaactaggt gtggaacgga tgaaaatcat gacagagacc accaatggtt ttgtactaag
     4561 cgaaaaagat ctggaattac gtggtcctgg tgaagtattt ggcttccgtc aatctggtct
     4621 gcctcaattt gcgattgctg acttggtgac ggatggaaat gttttggagg tagccagaga
     4681 agaggctgca gccctttggc aaataaagga ttggcagatg cttcctgagt atcagccttt
     4741 agcgcaaagc ctccaaatag aaaatcagga taatcgttat ttcgattagc agactgtgct
     4801 atactttata ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg tagatgcaat
     4861 gggcggagat cacgcacctc aagcaattgt agaaggcgtg gctttagcgc agcaagattt
     4921 tccagaagta gaattcttac tttacggaaa agaagcagaa atcaagaaat acttaacaaa
     4981 tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc aatagtgatg atgagccagt
     5041 aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt gctgcacaag cagtgaaaaa
     5101 cggtgaagca gatgctgtat tttcagcagg gaatacaggc gcgctactag cagcagggtt
     5161 gttcatcgta ggacgtatca aaaatatcga acgccctggc ttgatgtcta ccttgccagt
     5221 agtagggaaa gaaggtgccg gtttcgatat gctggatctt ggcgctaacg cagaaaacaa
     5281 accagaacat ttgttgcaat acggtatttt aggttctttt tatgcacgta aagtccgagg
     5341 tatcggacgt cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa aaggtagtga
     5401 agtgacgaaa aaagcctatg aactccttca gaatgaaaca agcttgaatt ttattggaaa
     5461 cgtagaagct cgtgagctac tcaatggtgt ggctgatgta gtggtcacag atggatttac
     5521 aggcaacgct gttttgaaat ctattgaagg aacagcaatg aatatgatga atctattaaa
     5581 atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt taaaagacgg
     5641 cttacgttct ttgaaagcag aaatggatta ttccaaacat ggcggtgccg ttttgtttgg
     5701 cttgaaagca ccagtcatta aaactcatgg agcaacaggc cccgatgctg ttcgctatac
     5761 gatccgccaa atccacacaa tgcttgagac agatgtagtt ggacaattag tcgaacaatt
     5821 tgaaaaaagc gaagaatagt atttggatat ctgaagcgat ccgttccatt gaaaaaaagg
     5881 tggacattcc tgaaaaatgc cagtaaaatt agccatggtg taggtaattg acaagtgtat
     5941 gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta gcgaaaatta
     6001 tttcaaatca ttttgagata gataccgata aagtgacgga tgaattgaac atcaaagatg
     6061 atttaaaagc tgattcaatt agtattatgg aattcgtttt agagcttgaa gatgaattcg
     6121 gtacggaaat ttcagatgaa gacgctgaac aaattgaaac agtcggtggc gcagtagatt
     6181 atatcagcag ccatcttaaa taatttcaaa taaaagcaaa gaaggat
//
LOCUS       DAHEYX010000154         6190 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHEYX010000154 DAHEYX010000000
VERSION     DAHEYX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.155"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            281..637
                     /locus_tag="MB219_002113"
     CDS             281..637
                     /locus_tag="MB219_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCC8427820.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            647..1546
                     /locus_tag="MB219_002114"
     CDS             647..1546
                     /locus_tag="MB219_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCC8427821.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2063..2794
                     /locus_tag="MB219_002115"
     CDS             2063..2794
                     /locus_tag="MB219_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCC8427822.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2939..4966
                     /locus_tag="MB219_002116"
     CDS             2939..4966
                     /locus_tag="MB219_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCC8427823.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5074..6171
                     /locus_tag="MB219_002117"
     CDS             5074..6171
                     /locus_tag="MB219_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCC8427824.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2146 a         1012 c         1273 g         1759 t
ORIGIN      
        1 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
       61 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
      121 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
      181 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
      241 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
      301 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
      361 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
      421 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
      481 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
      541 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
      601 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
      661 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
      721 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
      781 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
      841 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
      901 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
      961 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
     1021 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
     1081 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
     1141 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
     1201 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
     1261 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
     1321 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
     1381 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
     1441 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
     1501 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
     1561 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
     1621 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
     1681 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
     1741 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
     1801 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
     1861 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
     1921 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
     1981 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
     2041 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
     2101 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
     2161 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
     2221 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
     2281 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
     2341 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
     2401 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
     2461 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
     2521 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
     2581 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
     2641 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
     2701 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     2761 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     2821 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     2881 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     2941 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     3001 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     3061 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     3121 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     3181 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     3241 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     3301 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     3361 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     3421 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     3481 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     3541 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     3601 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     3661 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     3721 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     3781 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     3841 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     3901 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     3961 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     4021 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     4081 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     4141 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     4201 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     4261 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     4321 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     4381 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     4441 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     4501 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     4561 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     4621 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     4681 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     4741 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     4801 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     4861 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     4921 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     4981 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     5041 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     5101 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     5161 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     5221 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     5281 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     5341 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     5401 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     5461 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     5521 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     5581 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     5641 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     5701 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     5761 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     5821 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     5881 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     5941 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     6001 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     6061 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     6121 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     6181 aacaaatagt
//
LOCUS       DAHEYX010000155         6173 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHEYX010000155 DAHEYX010000000
VERSION     DAHEYX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.156"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            725..811
                     /locus_tag="MB219_002118"
     CDS             725..811
                     /locus_tag="MB219_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCC8427825.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="MB219_002119"
     CDS             complement(932..1621)
                     /locus_tag="MB219_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427826.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="MB219_002120"
     CDS             complement(1866..2780)
                     /locus_tag="MB219_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCC8427827.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="MB219_002121"
     CDS             complement(2762..3142)
                     /locus_tag="MB219_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCC8427828.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="MB219_002122"
     CDS             complement(3592..3879)
                     /locus_tag="MB219_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCC8427829.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="MB219_002123"
     CDS             complement(3899..4123)
                     /locus_tag="MB219_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCC8427830.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="MB219_002124"
     CDS             complement(4578..4748)
                     /locus_tag="MB219_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427831.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="MB219_002125"
     CDS             complement(4763..5296)
                     /locus_tag="MB219_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCC8427832.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="MB219_002126"
     CDS             complement(5317..6159)
                     /locus_tag="MB219_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCC8427833.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAHEYX010000156         6154 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHEYX010000156 DAHEYX010000000
VERSION     DAHEYX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.157"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(39..1334)
                     /locus_tag="MB219_002127"
     CDS             complement(39..1334)
                     /locus_tag="MB219_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCC8427834.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1539..3347)
                     /gene="pepF"
                     /locus_tag="MB219_002128"
     CDS             complement(1539..3347)
                     /gene="pepF"
                     /locus_tag="MB219_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCC8427835.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(3523..4521)
                     /locus_tag="MB219_002129"
     CDS             complement(3523..4521)
                     /locus_tag="MB219_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCC8427836.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(4747..5400)
                     /locus_tag="MB219_002130"
     CDS             complement(4747..5400)
                     /locus_tag="MB219_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCC8427837.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(5732..6130)
                     /gene="spxA"
                     /locus_tag="MB219_002131"
     CDS             complement(5732..6130)
                     /gene="spxA"
                     /locus_tag="MB219_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCC8427838.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1814 a         1264 c         1025 g         2051 t
ORIGIN      
        1 ggacagaaca gaaagaagaa aacctctttc tgttctgttt atttggttaa aaatgttttg
       61 ccatgacgaa tcataattcg tagtttgaaa ttgtaaaagt tgcgaaagcc gtacgcattg
      121 cgttttaata ctttgatgtg gttgtttaag cattccaatg gtccgtttga atagctgtag
      181 tgaagcgcat tttggatctg tggcagaaac tttttaaacg ttcgtaaagt ggttgtatag
      241 gtttctggca gaccgctaat gtctctagtt aaacaatcct caaatagtga ctgatctctt
      301 ttctttaccg cataaagaat gtcttggtat aactgatagc cttggcgtaa gttgggacaa
      361 gcagctaaca gtcgatcggc aatctcactt tcagtcaaat aatctttaaa actggcacgc
      421 caaacccgtt tttcatagtt taactttgct tcttcttttt gtaggagttt ccaatagcgc
      481 ttcaattttt tccctaaccc tcgttgagag tgggaatttc ctttaagtcg taccgtggtt
      541 tctttcactc gatgattcaa gaaagttctg ccaatatgtt ggacgatgtg gaaacgatcg
      601 atgatcaact gagcattcgg gaaaagcttt ttcaccaacg aaacataagg cgcatacata
      661 tcaataacaa tcaattgaac ggcttcacga gtcgctaatg aaaaccgaga aaaatagcgt
      721 tctaaataag gcaagcgtcg attttcgaca atatccaata atgcatggct ttgtccgtcc
      781 atcatgataa aactcattgc actggcaaca cttcgaaccg atttgaattc atcaaagcat
      841 aagacctttg gcaaagtctg tttgaaagga ttcaatgggc ggtagaatcg cttcatgaca
      901 cgatagaccg aagaagtgga aatatttttc tgacgagcga tttccgtcat ggaagtattc
      961 tcttttaagc gagcaatgac agaccaacga actttttgac taatgaaaca gtgtttttca
     1021 gcaacagagg tatccgctac gaatgttctt tggcaagact tacatttaaa tcgttgtttc
     1081 ttcagctcca gataagtgcg atactcagac acatcattca gtaacaaccg aacggttgtc
     1141 gtcccccaac gaatgatcct agaggggacg acacaaagac aatgggaaca ctcttctggc
     1201 ttgtacagaa gagttccttt gtaaatcaag caaatttgcc ctttgtattt ttctttagtt
     1261 aaacaatttt cctcgaaata gatatttagg tctagaatat ttagggtatc tttgataagc
     1321 tgagtatgag acatcttgaa tcctcctatg aattggtagc caaattaatt ctaaaggatt
     1381 tcagggtgtc ttttctatat ttttttatac aaaaaagatg ttggtgaagt tcaccaacat
     1441 caaaaagtat agagccttaa aaaaatacgt cgactccctt acaagagagc cgacgtattt
     1501 ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt ataaacgatc gatcaattct
     1561 tctaattcat ttagacgttg ttcaaacatc gacattgcat cttcaatata tgcagcctgt
     1621 gtcatatcaa ctcctgcttt tttcattact tccacaggat aatcgctgtt acctgctttt
     1681 aggtaagcca agtagttctc tagtgcctct ggttcctggt ttagtatttt cttcgccagt
     1741 gctgaagcag cagaaaagcc agtcgagtat tggaaaacat agtaattata ataaaaatgc
     1801 ggtatccgtg accattcgaa tttaatttct gggtcttctt cgactgctgg accataatat
     1861 tttgcattca atttaccata actatcactt aggtattcac tagtcaatgg cacacctttt
     1921 tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt
     1981 cctttaaacc catccaagta atggttgagt acataagccc gtacacgagg gtctttttct
     2041 gtttccaata aatactccgt taggatattt tcattcgttg tcgaagcaat ttctgctaaa
     2101 aagatggagt agtcgccgta cacataaggc tggttcgaac gagtgaaata actatgaaca
     2161 ctatgtccca tttcgtggac aagcgtaaat agctgatcca gcgtatcatg ccaattcaat
     2221 aaaatatatg gatttgtgtc atagcttccc gaagaataag caccgcttcg tttcccttta
     2281 ttttcgacaa catcgatcca acgttcagag aatgcttttt ctacgatggc catgtattct
     2341 tcacccattg gtttcagtgc ttctaaagct ttttcttttg cttcttcgta cgtaaaggta
     2401 attggagctt cccctaagac cggtgtataa agatcataca tgtgcagttt ttccacttct
     2461 aataaacgtt tccgtaattc catgtatcga tgtaacaaag gcaaatgctt gtttaccacg
     2521 tctaccaaag tatcgtatac actttcagga atatgattat tgctcaaaga cgcttctctg
     2581 gcagagctgt aattacggac tttcgcttta aaattatgtc cttttatatg tgtgcctaaa
     2641 gtagaagcaa atgtatttct aaattgttcg taaacactgt acaacccctt aaatgctgct
     2701 tcgcgcaccc ttcgatccgt gctttctagc aactgaccat acacgccatg agataattgg
     2761 actatttcac cattttctcc ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca
     2821 gcaaatgtat cacttgaagc atcaaagatt tcacctgctc cagcaagaag agattcctgc
     2881 tcagcagata agacatgggc tcgattatct actatttgct ggatataatg gcgatagact
     2941 tccaattttg gttcttcttt gaaatattgc caaatctggt catctgacaa ttgcaatatt
     3001 tctggttcaa accaggaaac ggcttcacta actttcgaaa acagactgct tgctcttgca
     3061 taaagcgctt ggtaatctgt atttccagtg tcttgatcgt ttttaaggtg cgcatataca
     3121 taaatgactt cagtttggcg ataaaccctc aatacgaatt caatcgcatt taaaaattga
     3181 gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt gttttaactc ttctgataat
     3241 tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt
     3301 aaattttcag gcaattcttc tcgttttggc aactgcttta cttccatgaa atccactcct
     3361 tttcttccct tagctctatc tatcaaaata gatcatagtc taacatccac atgtcacagc
     3421 atctaacttt tctactcatt catgtgtctg cttccatcaa ttttagcata actaaatctt
     3481 gttttacata gaaaaccgat tcctagccta tttttatact tactattttt tcaaagaaga
     3541 caagtgtatg cattcgttaa aaaactgctg atagaccatc tcttcatcaa tcataggaaa
     3601 aagccattcc cttttatgtt cttttatttg gcaaagccat gagttgaaat caggaacttt
     3661 ctggttttcc aaggcttctt cgtacaacat tcgataaaga aaaacctgtt cttgtaaata
     3721 aaagaaaaac cgactttttt gatagatcca cgatgataga tgcaaaagat gtttatgacg
     3781 caagtagcat tgttcttgta tggatactgt attcttttgc ttgtgaataa gttggcgtgt
     3841 cagccatttc ttcccatctt cactcacttt ttctattttt ttcatttggt tcattttaat
     3901 ttcgttacta gtgaatattt ctttaaggtc cctactagaa aaagtccagc agctactgca
     3961 aaaggaaata tgccctgata cagtctctgt aatatgatga tacaaataaa gcttttgttt
     4021 cttgagatca gctttccagc aatgcactcc tctttttcta ttatagtagc agcaactttt
     4081 ttctatcaat gagaattgcg attgtccaag aaaagaatta ccaagtatcc accatacttg
     4141 gtaactgtta ttcaaataat tttgggttct ttcaataaat ctctggtgag acagtctaga
     4201 acattggatt tctattgcta gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg
     4261 caaataggct tccaaaaata ctggttctac tgactgtccc agccaatcca tgaagcattc
     4321 ctttaactgt aagtgctctt ttgattctct ttcactaaag accgaacagt tatctgattt
     4381 ttgatgggca aaatgagcac attttttctt tccctttttc aaaatcactc tttttcgaca
     4441 gccacgacaa taatatttct ttcctttttc tgcatgttta gcaaaacagc acttattata
     4501 ttcattcatt gcaagcagca tttttctccc tctgaactta tttacgtcta agaatttatt
     4561 tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca agtaataaga acagctaaac
     4621 aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt accgcagatg caagcttttg
     4681 aacacattta tacgaaaaaa agactgcgac acaatttttg tcacagtctc ttactataat
     4741 gctgatttat acaaaataaa atttcgttcg ctcgatagca ttacgcgcca tcagacattg
     4801 tccatattca ttcaatgttt ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg
     4861 agcaatctga ttctctacat ccgcttggtt gagattttcc atccagaagt gaacgactag
     4921 gtaatagcgc tcttcgaata aatacaaatc agtccaagca gactctagtt cgacatctgt
     4981 tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc
     5041 ttctagggcg cttccagata attcttcctc cggatcgctg ccaataatct gctgacgaag
     5101 aaagtcactc acatcttctg aatcaacagt agaatcttct gattgagaaa tttcttcatc
     5161 cggaagattt ttactgataa acagttctaa tccgtctcct tttggcaaaa cttggaaagt
     5221 gacagcttcg cttcctcgga actcctcttc gatatcaact tcttccaaaa tactataaaa
     5281 gaaattttcg atttctttgt gattgccaag taaatccaaa aatgaaacgc cccgttcctc
     5341 taaatcttca tgaccaatta gtacacggat cgtattttca ttaatatgtt ccatttccat
     5401 tttgctacac ctcactttgc cacaatgttt aatatagatt cattgtagcg aaatcagctt
     5461 aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa taaaaccagc ggatttttca
     5521 acctttcaag ctatcttatt taactataac atttttggca gacgaatact atatagccga
     5581 cttttcagct aattttccct ctctattttt cgtgcaatca tcatcttcta ctaccttttt
     5641 atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt
     5701 aatagtcttt tccaagtttc gtattatcct tctataatcc agccatcaat tgtgcttgac
     5761 gtaattctaa ttgtcgtaca tcgcgcggta ggaaccgacg aatttcatct tcattaaaac
     5821 caacttgcag acgtttatca tcaatcatga ttggacggcg aagcaatcca ggattgtttt
     5881 gcacaagttc caataattct tgtaacggca gatcatctaa atccatgttc agtttttgga
     5941 atactttgga acgtgtcgag atgatttctt ctgttccgtc ttctgtcatt tgtaaaatcg
     6001 cttttaattc agaactgttc aatggttctg aaaaaatatt acgttcaaca aatggaatct
     6061 gatgttcttg caaccacgca cgagcctttc tgcaggaagt acaacttggg gaagtatata
     6121 atgttaacaa cgtgcaccac tccttttcgt gata
//
LOCUS       DAHEYX010000157         6124 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHEYX010000157 DAHEYX010000000
VERSION     DAHEYX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.158"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..582)
                     /locus_tag="MB219_002132"
     CDS             complement(<1..582)
                     /locus_tag="MB219_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427839.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEE"
     gene            complement(1150..2085)
                     /gene="whiA"
                     /locus_tag="MB219_002133"
     CDS             complement(1150..2085)
                     /gene="whiA"
                     /locus_tag="MB219_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCC8427840.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2119..3117)
                     /locus_tag="MB219_002134"
     CDS             complement(2119..3117)
                     /locus_tag="MB219_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCC8427841.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3114..3998)
                     /gene="rapZ"
                     /locus_tag="MB219_002135"
     CDS             complement(3114..3998)
                     /gene="rapZ"
                     /locus_tag="MB219_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCC8427842.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4133..4864)
                     /locus_tag="MB219_002136"
     CDS             complement(4133..4864)
                     /locus_tag="MB219_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCC8427843.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(4868..6079)
                     /locus_tag="MB219_002137"
     CDS             complement(4868..6079)
                     /locus_tag="MB219_002137"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCC8427844.1"
                     /translation="MNVYGMARSFNEAYGKICQAYASDQLAPTRYSKIVNVEVIPGFD
                     KDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQHKQELSEYFICPYIDYSLF
                     ERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQELPFEFPVALKPANSVEYLS
                     VQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFIPGDDSNMRVLNAYVDEDH
                     QVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKAFLEKIEYTGFANFDMKYD
                     PRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFNKPFVETTYGTNQSDKARL
                     WMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKDRSIKRWLLMKYMFHNYIP
                     RFKKYFHVKEG"
BASE COUNT         1781 a         1320 c          987 g         2036 t
ORIGIN      
        1 ttcttcttca tttattgtat tttgatcaag cggaccaaat tgttctaata attctttgtc
       61 atgtaaaata tcccttctag taatgcctct ttccctttca atgaaagaag cactaggaaa
      121 agtatgatta taaccattca acctatctcc catgtaataa ttagcaggtc ctggaaaata
      181 aaatatttgc ttattatcac taacttctaa cagcaacact aaatcttctc cgctggctaa
      241 acgacttgtc catctacgat atctccctat gccttcgttc attccaaatc catcaaattc
      301 aactattaac gtgtcttcta tcacatgtaa ttcttcattc aattcatact caagtctaaa
      361 aggaaattct ccatatgtaa tctctggcaa tggtggtgct ggagttctcc aaataaatac
      421 aataactgtg aatagaaaaa atccaacgat cgatataaat gttatgagac ctgctcgaag
      481 taaaacccga tataataaaa atgctgtatt tcgctttgct atatctagaa aaataaaaag
      541 tactgcaatc ataatgatag taaaagttgt aaaaaaacgc atttttgccc tccctttata
      601 aacatcgatt ctcaaattct aaaccaaatc ttggtagttc aagttcaggt ataacgtatt
      661 ccaaccatac atctcaatta ctatttatga acttatgatt atcactgaat aaatcactcc
      721 atccccttgt taaaacttct acattaatta cacctggttg ttttcgacta gctaatccaa
      781 tgaacttatc aaccatcctt atccaccaac cacgagaatc ttgaccagaa gtaagaagag
      841 cagttctcaa ggatgatcga gcagttcctt ccagtttttt aattaatcag gccagttata
      901 gataaatact tgatcttatc tatatcagga cttctatatt aagcaaaaat ttatgtacaa
      961 aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta aaaacaatag
     1021 aagcctttcc aaattatgtt actccagtaa agatggaatt atagtttgac ggaagaaaag
     1081 caagtataac ttttaaaaaa aactgataca ttcgcatctc ctgcttcatg tatcagtttt
     1141 ttctttggat taaactgctt cttcacgcag tttttccgca aattcattta ttttgcgaat
     1201 tcggtgatta attcccgatt ttgatatcgc acctgaggga atcatttccc ctaattcttt
     1261 caagctgacc tctggatgtt gaagccgaag ttctgcgatt tcctgaagtt tttcaggaag
     1321 ggcatgtaga ccgacctttg cttcgatcaa ttcgatgttt tcgatttgtt tagaagccgc
     1381 atcaatcgtc ttgttcagat ttgctgtctc acaattgact agacgattga ccgaatttct
     1441 catatcccgt acgattcgaa catcttcaaa tcggagcatt gagttggttg caccaatcaa
     1501 tgttaagaaa tcagcaattt tttctgctcc ttttaaataa gaaatatagc cattgcgccg
     1561 ttcaagcgtt cgagcatgta gaccgtaata atttaacata tcgcaaatgt cttggttatg
     1621 ctgttcataa atagaaaata tttccaaatg ataacggctc gtttccggat tattgactga
     1681 acctgaggcc ataaaagcac cacgtaagta agaacgcatt ttttgcgaat ttcccataat
     1741 ctcgtccgaa acgtgtccat tgaacatcat accatccatg atatcaagat ccatcaacac
     1801 atcttttgtg ccttgcttca accggacgat atagacattg ttctttttta atttcatttt
     1861 tttacgaacg agtagctcac ttcgtacctg ataatgatct ttcaaaagag aatagatacg
     1921 tctggcaatt gcagcatttt ctgtttggac gttcaaaata aactgctggt tcacaatacc
     1981 taaagagccg ttcattcgca gcaaagcagc taattccgct ttcgcatgtt ctcgatgaac
     2041 ttccaaacca gttagttctt tcttcacatc tgcagcaaat gacatcctat tctcctcctt
     2101 tctccaacaa taaattgctt aatattttga accgaaaact aaacggaaca attcttctac
     2161 tactttgtct ccatcgtgga agacaccacc atctttcaat tccaaaaagt cggtggaaat
     2221 cactcgacat ccttcatttc tcaatccgtt gaagtcgtgt gcgacttgta ctagatactc
     2281 atcatagatc tctgggtcca tataatcttc aggcactttt tccgtattga ccaatactgt
     2341 atcgataaaa ggctgattca aatgcttgtg taaaacacgt atatgatcgg catctgtgaa
     2401 atgttccgtt tccccttttt gggtcatgat attacagata tagacggttt cagcagttgt
     2461 cttgagcatt gcttcgccga tttctggtat cactaaattt ggaaggatac tggtgaataa
     2521 acttccgggt cccaaaacga ccatgtcggc gtccataatt gctgaaacga cttttcgagc
     2581 cgctttggct tcacttttat catttgtgtt gcagacatac acacggtcaa tggttttacg
     2641 gtcgatcgcg attttagatt ctcctactgc agtcgttccg tctttaaaga cagcatgcaa
     2701 ggtcaaagga gtttctgaag aaggataaac atgtccatct acatgcatca tttttgctaa
     2761 taattgaatc gcctcataag tgcttccccg catctcggaa actgcggcaa tgatcagatt
     2821 accgatcgta tgattggcta aaaactgatc ttcctttttg aaacggtatt ggaaaatatc
     2881 ttcatagaat tgtggcatat ccgataacgc cactaagaca tttctcaagt caccaggagg
     2941 agccatagcg atcgattctc tgattgcacc gctgcttccc ccatcatctg caacagtcac
     3001 tactgctgtg atatctgctc cttgatttcg caaacttttc aggataactg gcagaccagt
     3061 gccaccgccg accactacga tctttggttt tcttatccgg taggttttca ttttcacgac
     3121 cggttcaccg tctctttcct tttatccttg tcccgatgtg tcacattgac tttatagttt
     3181 ttccctaaag catttgccaa acgttcagtc aatgccacag aacggtgttg accgcctgta
     3241 cagccgattg caactgtcaa gctgcttttt ccttctttaa tgtatccagg caatacagtt
     3301 tccagaagat ccaaaaattt atggtagaat gattgcgttt catcaaagga catcacataa
     3361 tcatagactg gctgatcttt tccggtcaat ggtcttagtt ccgggatata atgagggttg
     3421 ggcaaaaaac gcacatccat cacaatatcc gaatcgatcg gcaacccgta tttaaaacca
     3481 aatgatacaa attcgatccg aaatccttgg tctccctttg atttgaatga ctcattgatt
     3541 ttttcacgta attgtcgtgg tgtcaatgtc gtcgtatcaa tgatcacgga agcttgggct
     3601 ttcaagtcgt ctagtatcgc ccgttcttta cgtatccctt cagtgatcaa accgtccatc
     3661 gccattggat gtgctcgtct tgtttctttg tagcgagcca ctagttcttc gtctgaagca
     3721 tctaaaaata agatagaagt gtcgatgaag ttcgtatttt cgatttcgat cagcatgttt
     3781 tggatttctt caaagaagga acgagaacgt aaatccactc ctaaagccat ttttgtgact
     3841 ttgcctgatt ctttgattaa ttcccagaat tttggaatca atcgaggcgg catattatcg
     3901 atacaaaaat agcccatatc ttcaaaactt tggatagcta ctgttttccc tgcaccgctc
     3961 atacctgtta tgatgactaa ttgcaaatta tctgtcataa ttacgaactc ctctcacatt
     4021 gtgcatagta aaattatatc attccgggcg tatcattacc agttttcttt ttcttttaca
     4081 aaaagatttg gaccaaatat cttttaggat atttgcccca aatccaattt ttttactttt
     4141 ccgatgctgt atccaatgcc ttgcttcttt cagctagcgc tcgttcaatc gtccgatctg
     4201 ctaaaataga ttgggcatcg atcactggat aatggttatc ttcagcaaat tcatgcatca
     4261 atgacaattc tgtgcaacct aagataactt tttcacagtt taggcgctct accgcttctt
     4321 ctaagatttc gtaatatagc tcttgattca aataatccga ttccttgatc tcatgataga
     4381 tcagataatt gattttttct tgaagaacag tatcaggaat cactgtttcg aatccaagat
     4441 ttttgacttc gcgttcatag attccagctt tcatacttcc ttctgttcct agaatcgcta
     4501 ctctccctgt cgtatgctga cggacaagtt catttgctgc ttctcttggc atatgaagga
     4561 taggaatatc cgttgccgct tgtaattctt caaaaaagta gtgtgcggtg ttacaagtca
     4621 acacaatgaa gttcggctgc aataaatttt gtttctcaat atcatccagc agaaacggca
     4681 ttggattttc ttctgatcga tctaaaatat aagctgtacg atcaggaact gttgcatgat
     4741 tgaataagac ataattcaaa tattcttgat cttttgtagc tttcgttcgg tggttgatca
     4801 agcgaacaaa actttctgtc gccatcgtgc ccattccgcc taaaatactg aaaaaattct
     4861 ccatgaacta gccttctttc acatgaaaat actttttgaa tcttggaata tagttatgga
     4921 acatatattt catcaacaac cagcgcttga ttgaccggtc tttttcataa aacacagtag
     4981 taccgtaacg attttctttg atcatttgct cagctagttt tttgtcttca ttttcccgag
     5041 catattctaa aaagattttt ttgggcacac ccatccataa tcttgcttta tctgattgat
     5101 tcgttccata ggtcgtttca acaaagggtt tgttaaacac tcgatcttct gtgacgaaac
     5161 gagccagatt caagccgttc aatgtcacaa agaaactgct acgaccttgt ctcaaattga
     5221 tttcaaataa tttatattct ccatctctag gatcatactt catatcgaaa ttggcaaagc
     5281 ctgtatattc gattttttcc aaaaatgcct taatggtttg atagattttt tcgttataat
     5341 ctggcatgat cacaacgtaa ttaccaatcg atgctggtgt aggatcttct aacagtggat
     5401 gaccaagaca catcatccga acttgatgat cttcatctac atacgcattt agcacacgca
     5461 tattactatc atcacccggg ataaaatctt gtacaatcat ttcactttta taaccggctt
     5521 cgtatatccg gcctaaaatt aaatcgaact cttctctagt ctctaaaata aaagcttttt
     5581 ttcttccttc aaattggaca gaaagatact ccacactgtt tgcaggtttc aaagcaactg
     5641 ggaattcaaa aggcaattcc tgttccaaat gtccatttac taacatttct tctctgacga
     5701 tcaatgtttt aggatacgga agatcatatt cttcacaaac ttcataaaag cttactttat
     5761 tgatcagtct ttcaaataaa gaataatcaa tatatggaca gataaaatat tctgacagct
     5821 cttgtttatg ttgggagatc aattctgcat atccatcacc acaagcaatc aaaagatatt
     5881 ttttactctt atcagaatat ctttcttttg ccaaacgaag catcgtctcg ataaacacag
     5941 ggtctttatc aaatcctgga ataacttcta cattgacaat tttactgtag cgggtagggg
     6001 ctaactgatc cgaagcatat gcttggcaga ttttgccgta tgcttcatta aaagaacgtg
     6061 ccataccata aacgttcatg tcgcttccta ataatatagg gataaattct tcattttcaa
     6121 tact
//
LOCUS       DAHEYX010000158         6061 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHEYX010000158 DAHEYX010000000
VERSION     DAHEYX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.159"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            34..2631
                     /gene="adhE"
                     /locus_tag="MB219_002138"
                     /gene_synonym="adhC"
     CDS             34..2631
                     /gene="adhE"
                     /locus_tag="MB219_002138"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCC8427845.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2860..3966
                     /locus_tag="MB219_002139"
     CDS             2860..3966
                     /locus_tag="MB219_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCC8427846.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3983..5007
                     /locus_tag="MB219_002140"
                     /pseudo
     CDS             3983..5007
                     /locus_tag="MB219_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5013..5492
                     /locus_tag="MB219_002141"
     CDS             5013..5492
                     /locus_tag="MB219_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCC8427847.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5508..>6061
                     /locus_tag="MB219_002142"
     CDS             5508..>6061
                     /locus_tag="MB219_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCC8427848.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1949 a         1075 c         1368 g         1669 t
ORIGIN      
        1 aacaaataac catgacccat taggaggata atgatggcta aagtaataga aaaagaaaaa
       61 acgaagacaa ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc aaatgcagca
      121 ttgaaagaaa tggaaagctt tgatcaagaa aaagtagacc atatcgtgca tgaaatggca
      181 atggcagctt tggatcaaca tatgccatta gcgaagatgg ctgtagagga aacaggccgc
      241 ggtgtttacg aagataaagc aatcaaaaat atgtatgcgt ccgaatatat ttggcataat
      301 atcaaacatg ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa
      361 attgctgaac cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc gacttccaca
      421 acgattttca aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt tgctttccat
      481 ccaagtgccc aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct
      541 gccggagctc ctgaaaattg tatccaatgg atcgaacatc cttcgatcga agcgacttct
      601 atgctaatga accatccagg tatcgcgatc gtcttagcga caggtggagc tggaatggtc
      661 aagtctgctt actctacagg aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct
      721 tatattgaaa aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca
      781 tttgataatg gaatgatttg tgcttctgaa caagctgtga tcgtggataa agaaatctat
      841 gcagcagtaa aagcagaatt ccaagcacac caagtttata tcgtaaaacc agatgaattg
      901 caaaaattag aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt
      961 ggacattctg cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa
     1021 atgctgattg ctgaattgga aggcgtaggc ccagattatc cgctatcaag agaaaaactt
     1081 tctccagttc ttgccatgat caaagctaac aacacggacc atgcgtttga tttgtgtgaa
     1141 ggtatgttga atcttggagg attaggacat acagcagtta tccattcaga aaacgaagag
     1201 ttgcatgtca aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct
     1261 gcagaaggcg gcatcggtga tatctataac gaaatgattc cgtcattgac tttgggatgc
     1321 ggttcttatg gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc
     1381 aaaactgtag cgaaacggag aaataacatg caatggttca aattaccgcc aaaaatcttc
     1441 tttgaaaaga actctctatt gtacttagag aaaatggaaa acgttgaacg tgtcatgatc
     1501 gtttgtgacc ctggtatggt ccaattcggt tactgcgata cagttcgtga agtattggca
     1561 cgacgtaaaa atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca
     1621 aataccgtat acgcaggaac gaaactgatg gcagacttca agccagatac agtaatcgct
     1681 cttggaggcg gttctgcaat ggacgcagct aagggaatgt ggatgttcta tgaacaccca
     1741 gacacatctt tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg gacatataag
     1801 atcgacaaac cagaaaaaac acaattcgta tgtatcccga caacatcagg gactggttct
     1861 gaagtaacgc catttgcggt catcactgac agtgaaacac atgtgaaata tccgttagca
     1921 gattatgcat tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca
     1981 gcatctgtca cagcagatac cgggatggat gtgttaacac atgcaatcga atcctatgtt
     2041 tcagtcatgg cttcagatta tacacgtgga ttgagcctac aagcaatcaa gttagtcttt
     2101 gactatttag aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa aatgcacaac
     2161 gcttctacaa tggctggtat ggcgtttgct aacgcattcc taggtatttg tcactcagtc
     2221 gcacataaaa tcggcgggga atacgggatc cctcatggac ggacaaatgc gattctttta
     2281 ccgcatatta tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat
     2341 gactatttcc gtgcagatac agattacgca gatatcgcaa aattcttagg tctgaaaggc
     2401 gaaacaacag aggaattagt cgaagcttta gcaacggctg tttatgaatt aggaaaatct
     2461 gtcggcatca acatgagtct aaaagctcaa ggagtcacac aagaaacact agatacaacc
     2521 gtagaccgta tggctgaatt agcttacgaa gaccaatgta cgacagctaa tccgaaagaa
     2581 ccattgatct ctgaattgaa acagatcatc attgatgctt acaacggata agcaaaagaa
     2641 agcgtagaaa aaagataaga aaatagatga aaaagaactt ggaaaacgtt gtttctccaa
     2701 gttctttttt taatttccaa gtaactgaaa gttacttttg ttatttaaca tttaggtcaa
     2761 ttttagacag tttttgttca aaaagcgata tttatagtgg acggtattac tgctatatta
     2821 aggatataaa atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt
     2881 tttgtaccta aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg
     2941 atcaaagacg ataatggtga atttttgttg gactttgatg gactgaaagt agatgacaaa
     3001 agctggacag tgtggaattg gccacaaggt gtcggtctat atggtattta taaaaattac
     3061 cagatgacaa aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg
     3121 gaagaaggcg ctccgcccaa aaacgtcaat acaatggcac cgctattgac tatggcttac
     3181 ttgtatgaag acacgaaaga ttcacgctat ttgccttatt tagaacaatg ggcagaatgg
     3241 gtcatgaatg acatgcctcg gacaaatgaa gacgggttac agcatgccac atatggaccg
     3301 gaaaataaga atcagttgtg ggacgataca ttgatgatga cagtgcttcc tttggcaaaa
     3361 atcgggaaac tgttgaatcg cccagaatat ttagaagaag ctcgttacca gtttatgatc
     3421 catatcaaat acttgatgga taaacgaaca ggattgtggt atcacggctg gacgtttgaa
     3481 ggaaatcata actatgccga agcattttgg gctcgtggaa actgctggtt gacgattgct
     3541 attccagaga tcattgaaat cttagaatta tcgaaagaag atgcattgcg caaattattg
     3601 atcgaaacac ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga aagcggttta
     3661 tggcatacgt tgttggatga tccttcttct tacgtagaat cttctgcaac tgcaggattt
     3721 gcttacggta ttttgaaagc ggtccataaa cgttatttac cacaagaata taaagaagtc
     3781 gcttatcgtg ctatccaagg attgcttgaa caaatcgatg aaaaaggaga agttcaaaac
     3841 gtttcaatcg ggacaggaat gggggacagt ttagattttt atcggaatat cggaatcaca
     3901 gcaatgcctt atggacagtc gttaacggtg ctttgtttga cagaattgct cgtctcttat
     3961 tgttaaaaga aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag caagttcgct
     4021 tagcctaggt ctgctaggtc taagcggctg cggaaataat tctagtgtgg caactgtcgc
     4081 agatgcagac gtaaatgaga tagttacctt gcgctttgct tatgcaagca acagccagta
     4141 attgatgcaa tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg ttccgtacag
     4201 atccagtatt tcccagatgg acaactgggt ggagaacgtg aactgatcga attgacacag
     4261 tcaggagcag tagatttcac aaaagtaagt gcctcagcgt tggaaagttt ctctaaagat
     4321 tattcgattt tttccgttcc ttatctattc aatgatgaag aacatttctt taaggtgatg
     4381 gataatgaag aaataatgga gccaatctac caatcgacca aaaatttagg atttaccgga
     4441 ttgacctatt atgattctgg tcagcgcagc ttttatatgg tagacggtcc gatccatacg
     4501 ccggaagatt tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc tatcaaaatg
     4561 gtagagcttt taggcggttc gcccgtacct atgggaagtg atgaagtgta cacgtctctt
     4621 caatccaatt tgatcaacgg atcagaaaac aatgaatttg tcttgtatac agctggacat
     4681 ggcggggtag ctaaatacta ttcctatgat gagcatacac gtgtgccaga tatcatcatc
     4741 atgaatgatg ccatcaaaga acagctaact gatgaacagg aaaaagccat tgaagaagca
     4801 gcgaaagaat caacggtttt tgaaattgaa gcttttgcaa aagcaattga agaagaaaaa
     4861 gctttagcac aagaagaaca tggagtccaa ttcaatgatg tagataatac accgtttcga
     4921 gaggcagttg ccccacttca tgatatattc aaaaacgatc ctacctacaa agacctttat
     4981 caaaagatcc aagagcaagg agcgtagctg taatgaaaat gattttaaac aaggtattag
     5041 agatcctggg agctgttttg cttgttggga tggtcgctgt tgttttatgg caagtatttt
     5101 ccagaacggt tttaggaaat ccaaatacgg tcacggaaga gcttgttcga ttcggtcttg
     5161 tctggtttgc catgttgtct tccgcgtatg ttgtgggaca aaaagcacgt ttagccgtaa
     5221 cggttttaag tgaaaaatta actggcaaaa aaagtaaaat tttagaagtt gttgttcaaa
     5281 gtttatttct ggtatttgct gcgatgatca tggtgtatgg cggatggaat gctgtcacat
     5341 tgacaatggg gcagatctcg ccttctttaa gtattcctat gggatatgtt tatctgtcag
     5401 tacctgtatc gggggtactc atcattatct atagcctgat caatctaatc gatacattaa
     5461 gcaacaagcc agcaattcaa gaaaaaatat aaaaatggag gaataaaatg gcaatagaag
     5521 ccggattagt gttatttttt gtatttgttg tgttgcttgt attagggatg ccgattgcta
     5581 taagtattgc attctcatcg atgatgactt tattgttagt tataccgttt gatgtttctg
     5641 cttttagttc agctcaaaaa atggtgggaa gtatcaatag tttttcgctg atcgctattc
     5701 cgtttttcgt tctctcgggc attatcatga ataatggagg gatagctaag aaactagtgg
     5761 attttgcgat gttgttcgtc gggcgcgtgc cgggggcttt ggctcatacg aatgtgttag
     5821 ggaatgcttt atttggttcc atctctagct cggccattgc cgcttctacc gcgattggtg
     5881 gagtgttgat ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg acagcggtca
     5941 atatcgcgtc agctccgaca ggtatggtca ttcctccaag tactgctttt atcatgtatt
     6001 cacttgttgc tggtggtgct tcgatctctg ctttgtttat gggaggttat ctcgtaggcg
     6061 c
//
LOCUS       DAHEYX010000159         6055 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHEYX010000159 DAHEYX010000000
VERSION     DAHEYX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.160"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..476)
                     /locus_tag="MB219_002143"
     CDS             complement(<1..476)
                     /locus_tag="MB219_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCC8427849.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYY"
     gene            complement(617..1219)
                     /locus_tag="MB219_002144"
     CDS             complement(617..1219)
                     /locus_tag="MB219_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCC8427850.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1465..1671
                     /locus_tag="MB219_002145"
     CDS             1465..1671
                     /locus_tag="MB219_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427851.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1984..2703
                     /locus_tag="MB219_002146"
     CDS             1984..2703
                     /locus_tag="MB219_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCC8427852.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2784..3467)
                     /locus_tag="MB219_002147"
     CDS             complement(2784..3467)
                     /locus_tag="MB219_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCC8427853.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3699..5885
                     /locus_tag="MB219_002148"
     CDS             3699..5885
                     /locus_tag="MB219_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCC8427854.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2272 a         1018 c         1149 g         1616 t
ORIGIN      
        1 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
       61 tgaaaccaag tctctaacga ttttttaaaa caaaaagcta ataaagcaga taaaaaagcg
      121 gataaggcaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
      181 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
      241 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
      301 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
      361 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
      421 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
      481 tacagctccc attgattttt accaaattat agcacaatta gcgtattatt tttttaattt
      541 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
      601 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
      661 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
      721 tagaaaagta agaagctgag aaaaataaag taatgagaga aaaaattaga aacatgctga
      781 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
      841 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
      901 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
      961 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
     1021 atatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
     1081 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaaggca aattttactt
     1141 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
     1201 tcacaacttt atctttcatc tcatacgtac ctctaccaca aattttatgc taaaaaaaat
     1261 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatttccta
     1321 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
     1381 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaaat tactgacctt
     1441 ataaaaggtg atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca
     1501 tatgcattta aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat
     1561 gaaaatagtg tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat
     1621 aatctgcact ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata
     1681 aaatgttgag agcataaaga ggttgtgagt ctccgtttag ctctgagtta caaggaataa
     1741 tgttaaaaaa cagcttttca tgttttatga atattgccac ttgtgaccga ggtgttggca
     1801 ggagaatact gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt
     1861 tcatgaatag gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa
     1921 gtcaaggctt ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga
     1981 aggatgccga taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacactg
     2041 actgaaatgc ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat
     2101 gaagtattgc cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc
     2161 tttttgaaca aacgcttttt ggcaatcaaa gatgctttca gccatgatga caatgtacta
     2221 gaccgctcca tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt
     2281 tctgaagtag aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa
     2341 gaaatttcgc cacaaaagaa accagatcta ctggtttata ttcgtgtatc ttttgaaact
     2401 atgttagcga gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa
     2461 ctctattcct actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat
     2521 atatgtccca aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt
     2581 ggagaacaaa tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat
     2641 gcccagtcga tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa
     2701 taatataaaa actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc
     2761 ttagtttttt tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat
     2821 aaatgaaatg tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa
     2881 tgggcgccaa acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga
     2941 acgacactta gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa
     3001 tcatataagt tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca
     3061 cgcccgttga ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc
     3121 gttactaatg cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct
     3181 tcataatata gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt
     3241 agatactttg ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca
     3301 acacttagcc attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga
     3361 gcagaggcaa aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat
     3421 gtcagcaaaa gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc
     3481 ttttatgaat atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt
     3541 ttcagaaaac taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata
     3601 aagaaaaaat agacaacgaa actttaaata accaacttga aataaccaac ttgaaataaa
     3661 caaattcaaa gaagaaagat gggaaggaaa tcaatcagtt gaatgaaaaa gaaattctcc
     3721 gattacaaaa agaattgaaa cgctgggaga acaagtataa aaagcttcaa caagtctttc
     3781 ggctacaaga agaaatcatt gctacctatg aagcggctta tggcgaagca cccggactta
     3841 ttgaggaaga agtacaagta aaagaaagtg aaggaaacag ctctttcttt aaaatgatgg
     3901 agaaacgttc ctataatcgc ttcaatcaag aacaaaaagc agctatcaca tatgatatga
     3961 agaaacatct tcgaatcata gcaggagctg ggagcggcaa aacgcaaaca atctgtgcaa
     4021 aagctgttta tctgatgacg caaaaacaag tagacgaaga acgcatacta atgatcactt
     4081 ttacaaggaa cgctgcaaat gaactaaaaa aacgagtaga taatttcagc cagagaaaaa
     4141 ccgctgtcca tatcggaaca ttccatagta tcttttttag gatatacaat gaaatttgtc
     4201 gaaaatttcc tgaagtagca atgcaaggaa tacaaggaga cttttcaaag gatactgcac
     4261 aaaaagtgaa tgctgttttg caacagctga tacgtaaata taatctttat atatttgatc
     4321 aatatggaga aaaaacgatt gcttctcggt tagactattg gcagaatatg aacctttcag
     4381 tagaagaaat ggttcagtta gttaaagaaa aatatgattc cattgataag aatgcacgac
     4441 agccgatatc agaacgactg tatggattgc tcacggaact ccaagaacaa aaaagaagcc
     4501 agcaattgct agagttcaat gatgtactcc aaaatctgaa aagcgctttg gagaatgagg
     4561 agatccagca ttatatcggc caaaaatatg attatctatt cattgatgaa tttcaagata
     4621 ctaatccact tcaatgggaa atcgtgaaac ggatgacaaa ggaaaacagg atcaagttga
     4681 ttgttgttgg agatgatgat cagagtatct atggattcag aggatcagag ccagcataca
     4741 tcaagaactt tgaaaaagaa tatcctacca aaactctttt cttattaact aattatcgtt
     4801 ctcgggcagc tattgtccaa ggagcaaata gattgatcag ctataataaa aatgatcgga
     4861 taccaaaggc gatgattcca gcacaaaaag aagcaggtgt tatggaagcc tatttgtttt
     4921 ctgatactaa agcagaaagt cagtggctaa tcaaacaaat tcagacattt attcaaaaaa
     4981 atgggaaata taaggaatca atcatattat accgatcagc ttcacaaacg cagcagcttg
     5041 tccaatctct gctgaaaacg aatattcctt ttgtactgga agcagatagt ccatatgaag
     5101 gtatctttgg tatcaagtct ttccaacatt tctatcaaaa aattgttcat tggcaaacag
     5161 cactgaatct gcaaaagaaa aatcaggcct accagcaatt gctccggcag ctgatggcag
     5221 actgctacct gaaaaaaagt gaatgcgatc aatacttttc tcctaaatat caaaatatag
     5281 ccgttgcaga ttatattttg tcgataagcc ccaatttaaa atcaaaatct gcagaaatcc
     5341 gagagtttga ggaagcgttg cgtaaaacag accagcatca agaaatctat tcgcttgttc
     5401 aaagctatct tcatctgcca cgtatagcta aggaaatcga ccagagtgat caagaatgga
     5461 tacgagaaga agtaaagcag catgctactt tcagaagact ccaagcctta tcgcaagaaa
     5521 cccaagcgac agctaaagaa ttaaaaaccc gcttgattgc ttacagacaa ggaaaacttg
     5581 atgcactatg tatccagagc attcacaaaa gtaaaggtct tagttaccga aatgtctttt
     5641 tgattggttg taacgaagga agtttgcctt ataacggagc gacagaaaag aaagtaatcg
     5701 actttaatga aataaaagca gaaccagtga cgacgataga agaagaacga agactatttt
     5761 atgttgcgat gactagagca agaaatcgtc tgtatctatg tgtacctcaa atgaaaaatt
     5821 caagaaaatt gaaagtatcg agatttatca aagaaaccgg gagtcgtgtg aaaaaaggaa
     5881 agtgatagaa gagagggtgt gacagaagtg gtcagtttca agaaagaaga aggaatgtac
     5941 gaaaattgct tctcaaattt ttgtgaaatt tcagcttatt ttcgaagaag ctatttctgg
     6001 aacaccgttt attcggaaaa aggaggtgtg acaagaattt tgtcacacct ccctt
//
LOCUS       DAHEYX010000160         6037 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHEYX010000160 DAHEYX010000000
VERSION     DAHEYX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.161"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(701..1204)
                     /locus_tag="MB219_002149"
     CDS             complement(701..1204)
                     /locus_tag="MB219_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCC8427855.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1563..2588
                     /locus_tag="MB219_002150"
     CDS             1563..2588
                     /locus_tag="MB219_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCC8427856.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2911..3789
                     /locus_tag="MB219_002151"
     CDS             2911..3789
                     /locus_tag="MB219_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCC8427857.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4265..4381
                     /locus_tag="MB219_002152"
     CDS             4265..4381
                     /locus_tag="MB219_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCC8427858.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5309..5647
                     /locus_tag="MB219_002153"
     CDS             5309..5647
                     /locus_tag="MB219_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427859.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5741..5971
                     /locus_tag="MB219_002154"
     CDS             5741..5971
                     /locus_tag="MB219_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427860.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2064 a          898 c         1109 g         1966 t
ORIGIN      
        1 tatcgaaaaa tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa
       61 aaaattagtc aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga
      121 ataaatatta acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata
      181 acatatctca ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac
      241 cgtgtctcag atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt
      301 caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag
      361 gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat
      421 aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga gatattgact
      481 aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag
      541 gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt
      601 agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac
      661 attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc
      721 gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg
      781 ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac
      841 ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga
      901 ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag
      961 aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca
     1021 agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga
     1081 cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga
     1141 ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc
     1201 gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa
     1261 aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt
     1321 gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt
     1381 ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac
     1441 tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca
     1501 aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga aggagggata
     1561 gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga
     1621 atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat gattttttga
     1681 tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga
     1741 aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg
     1801 ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt
     1861 ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga
     1921 tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac
     1981 tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg
     2041 attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc
     2101 ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca
     2161 gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc
     2221 tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg
     2281 gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt
     2341 ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg
     2401 ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact
     2461 tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag
     2521 gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat
     2581 tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc
     2641 tctaattcaa ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg
     2701 tggaaaggaa ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg
     2761 gtaactgaat aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc
     2821 tttcttcata gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat
     2881 atggtgtccg gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat
     2941 ttgatgaaga aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa
     3001 gtcgttatag agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa
     3061 tatagtcatg aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa
     3121 gtctatgaag aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt
     3181 tggaaaaaaa tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat
     3241 gcttttacta gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt
     3301 ggtttttgga tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac
     3361 gaaatgacat attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt
     3421 tctcttcata ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa
     3481 agtgccattc gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca
     3541 gatacttggt tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt
     3601 ctttttgcag ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct
     3661 tggatctttg gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag
     3721 cgacaagcaa aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt
     3781 acttcataag tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca
     3841 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa
     3901 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg
     3961 agttaaacgc ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca
     4021 ataggtaaac tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag
     4081 gtagctaaaa cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg
     4141 atcgactgtt ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat
     4201 cgtaattgtt tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt
     4261 ccatatggaa aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa
     4321 aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta
     4381 agaagaagga aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa
     4441 tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga
     4501 taaattggta gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt
     4561 aactacagaa ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat
     4621 tccctttcaa cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg
     4681 ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg
     4741 cgaaatattt catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta
     4801 tcgtttaata agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc
     4861 ttttcttcaa aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta
     4921 aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa
     4981 catcaaggaa taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt
     5041 ttagatacag taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc
     5101 tttaataaaa ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt
     5161 cagtcgattg tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga
     5221 agataaaaaa tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt
     5281 ccctctgttt agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga
     5341 taaccttttt cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac
     5401 tccattagct agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca
     5461 gtacgaagtt atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct
     5521 aacaccgatt gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga
     5581 gactgagatc attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg
     5641 gatgtagaca ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta
     5701 gcacaagaaa tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa
     5761 agaaaatatt tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga
     5821 attcaagagt cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc
     5881 gatagaaaaa actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga
     5941 gtttttggat tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca
     6001 catattatgt tattgttttt tcgatatctt tgctgtt
//
LOCUS       DAHEYX010000161         6030 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHEYX010000161 DAHEYX010000000
VERSION     DAHEYX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.162"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(92..1222)
                     /locus_tag="MB219_002155"
     CDS             complement(92..1222)
                     /locus_tag="MB219_002155"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCC8427861.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1282..1509)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1871..3073)
                     /locus_tag="MB219_002156"
     CDS             complement(1871..3073)
                     /locus_tag="MB219_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8427862.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3133..3336)
                     /locus_tag="MB219_002157"
     CDS             complement(3133..3336)
                     /locus_tag="MB219_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCC8427863.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3478..4710)
                     /locus_tag="MB219_002158"
                     /pseudo
     CDS             complement(3478..4710)
                     /locus_tag="MB219_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4873..5685
                     /locus_tag="MB219_002159"
     CDS             4873..5685
                     /locus_tag="MB219_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427864.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1784 a         1244 c          967 g         2035 t
ORIGIN      
        1 taatataaaa ggtaaataaa aaagagtgga tttctccact ccatgaaaag ccgacttttt
       61 gggacgcttt tttttgccaa gcctcacatt tttatagcca aacttctttt gcgacttgct
      121 gcaataattg gattttcttc cattgttctt cttctgttaa atggtttcct tcttctgttg
      181 aagcaaaccc acattgagga ctgatactta gattttccaa tggtacaaat tgttgtgctt
      241 cttttattcg ttcttttatt ttctccgtat cttccaaatc tggaaactta gaagtaacta
      301 atcctaaaac aactttactt tctttgttac tccaatgtct taaaggagta aaatctcctg
      361 ctcggtcaga atcgtattcc aaaaagaaac catcataatt tgttgaaaac aactcttctg
      421 caatgggttc atatccaccg gaaaacagcc aagaagaagc gtgattcccg cggcagatat
      481 gtgttgtgat cgtcaaatct tctggtttgt cttccagtgc gttattgata atctctgtac
      541 aaatatgggc catttctttc ggatcaattc ctttttgaag aagtgcttct cttttagttt
      601 gatcagctag aaacgcccaa tttgtatcat cgaattgcag gtaacggcaa cctttttcat
      661 aaaaagcatg aatcgctgct ttgtaagtag aggacaagtc ctcataaaaa tcagatagat
      721 caggataaat ctccttgatt tttgaagaac catcattaga taggatctct tgtcgtaaaa
      781 tcatcgtggg actagggatc gttgcttttg caacaaaagt atcatctgtt tccactactt
      841 ctttcagaaa agcaaaatct tttaaaaatg ggtgatttgg attgaaagat aatttatcta
      901 ctacccgaat attataagct ggtgctgctt ttccgtggaa tgtttgatta tatccagttt
      961 ctggaacata gccttcgata ccatttagtt cttctaaaaa atcaagatgc caaaatccac
     1021 gccgaaattc accatctgta attcctttga tgccgactgc tttttggtct tccactaatt
     1081 tttttatttc ctggtcttcg attgcacgaa gttcttcttt tgaaagtaag ccttcttgaa
     1141 ctttttttct tgcttctttg atcgattgcg ggcgtaagaa acttccgaca tgatctactg
     1201 taaatggtct atttttgctc atttcactca ctcttttctt tgttttttgt aaaaaaaaat
     1261 ccttttacta gcaaatacta gtaaaaggac gattatactc gtggtaccac cttttttcag
     1321 aaaaaacatt ctttctctca gcctgacaat aaaatcagtg gtcttggtaa cgaaagactg
     1381 ttcgtttttg cctacctttc gacaaaaaat cctctaaggc cattttcgac agtcaacttc
     1441 tagcttttta caccaaccaa agcctctctt gaaccatttt ctgtctactt tccttctcat
     1501 cggatactat tagaataaaa ttagaatagc atgtttgaaa gaaaagtcaa tagggaataa
     1561 gaacaaacaa aaactaattt gttattctta gtaatttgtt tgtggaatac aataaagctt
     1621 ttctaatatg ataattgttc agtcgtcctt tcatcttaga tcacttcctt aaaatgatca
     1681 tacccgtttc aaccacataa aaaaggactt aattttgaaa aatttcccat caaaactaag
     1741 tcctttttat tatagttatg caaatttgct tattttctcg ctatttccga tacatcttga
     1801 acaggagcaa ttcgactgaa aacactggtg caaaagcatt tatttgtctt atggtagaac
     1861 cgtggaaaac tcagactggt aataaattgt cattttttga aagaatagcg ccctcttgaa
     1921 cattatcaat cgcacttacc acttgttgat ctgactttgc atataagtga ccgtaagttc
     1981 ctaaagtgat ttgaatattc gcatggccca atcgttcttg aatagatagc ggttgaatat
     2041 tctgctcaat tagaaatgca acatgagaat gtcgtagcgc gtgaagctta atcggtttga
     2101 cacccgcttt tttagcagct tcttttaacc agttcgcaaa agaataaggt ggcgtaaatg
     2161 ttccgtccaa ctgagcaaca tattcaaaat ttccaatacg tttttgcaag tcataccact
     2221 gtctgagttt taaggaaagc ttcttcccca acccaatcgt tcgataagca catttagttt
     2281 tgggagtcga aaagtaccaa tcatcttttg tattataaat caatgtcttc tcaatcgtta
     2341 tcgtgttttt ctcaaaatct acattcgacc aagacaaagc ttcaagttct ccaatccgaa
     2401 tgccagtata aaagagcatt tcatacacaa tcttccgatg gtgggtttga atcttatcat
     2461 ccttcaattc attcatcact ttttgaaact cactgtgttc ccaaaactca actttcttat
     2521 tctcagaagg aagcgaagaa acatcttcaa cgggattttt agcaatcacc ccctctttaa
     2581 cagcccgatc caatacaatt tttaggtgcc ctcgaatccg attgatataa gattttgata
     2641 acgtttcgcc attaactttt ttccgtgaat tacgtgactc tgtctggctt agttcaaata
     2701 accaatcctc aatatcgtga cttgtaatat ctcgaacacg aaaatgatag aaaaactgca
     2761 tacgcttttc gaaaatgaat tgcgcattct catatgtttg acttttgacc tgtctctcgt
     2821 accattttac aaacctatcc ttgtaaaact cttcgaaagt catttgattg gtagaacaca
     2881 gttgattagg aattgtcttg ttaaaatcat gtgtttccat gtacttgtca taagcaattt
     2941 tggcatcttt ttgtgaagca taacctcgtt tagtcttctg aacctttctt ccattttcat
     3001 ctatacctag atacgctcgg aagaaccatt tttttgtctt tttatcttgg taaatatttt
     3061 taatttttgg catcgttcca ttcctccctg tacttgagaa aagctacttt aatagtaccg
     3121 cgcttgtact gtttattccg caacttgaat gtgaaaaaga tgttcaatta ctcgggatgg
     3181 caccacacca acttggcgat tattataaaa atcaatacct tcaatccttg caagatatgc
     3241 cttgctctct ttaatcattc gtgcagcttg actaggttta aagcccatct ctatcaaaat
     3301 tgcacgattt accatgaaat cacattttgt ttccattctc tcacctcctg attctttact
     3361 ccttttaaaa taatcctctt tataatctat tgtttctcat tcgatggttg tgttatactc
     3421 gtcttgtcga ggcggagtac gttgcacaac ttgttgtgta agcagtactt tgctttttta
     3481 gtagcttgtt ttctcatttg atatactttg taattttcgt tcataatact caacctcctc
     3541 ctgataatac tccgggtctg ccttcatatt ttggagaagt ttgatctgtc ttggattcat
     3601 ttctccttgt tcaataatca aaggcaacaa ggaagaatga aacgtttgat cgacctgtgc
     3661 aataaaaact aatgatggtg aaacaccatg aattaaccaa ctcatagttt tctcaaaact
     3721 ttgaggaatt gtatctaaag aaatggtgat tgcgccataa ttgctaataa aggctgccca
     3781 tttggcatca acttttgcac ctagatcatt tggatcacgg tcataaaaac aaattgctcc
     3841 attaagtaac tcaaagaaca gtttggttaa atcatgggtt tttgaaataa tctttgccaa
     3901 gctttgcgct ttctcttttc gataccgtag ttcaaagcga ttctttactt ttgcctcttc
     3961 taaaggaatt cctcgtcgct tccgttgttc atggtccttc tgataaaagc aaaaacgaca
     4021 ctgactcttc tttgagccta aatacaaggt ctcaccattt gattcattgt ttatgatatc
     4081 cgtaccgcca tgagattcac tcacttgaaa agtggtccac acatggccta gtattacctt
     4141 atgcttaagt tctggaatgc tgagactccc cacaaaatca tctaaagtaa aatcaatccg
     4201 tgtaaagttc ccctgataat ctaatacctt acgaaaaaac tgttgccaat ttgtttttcg
     4261 tgtttttagc catcttgcta agtgtttaca gccttgacct gaaaactcca tcacagttcc
     4321 tttaaccgca tcgtcaatgg aataccgaat cgtgattact tgattccaca cgtattggcc
     4381 aatatagccc tttggcgcat aatccagttc ctcaaaacgt tcaatgggca tttcaataat
     4441 ctcttcaatt agctttaaat gttgattcga gaaaaagtga atggttagat aatcaatcaa
     4501 aacacggtta actggaacga cctcttcttg caaactatcc aacacaagat tcaatttttc
     4561 ttcacttaat tgtctcgttc ctttttctac tttcgataat aactatctcg aaatccccac
     4621 tttttctgct aaatatcgtt gtgtccaccc cctttcttct cgagaaactc gaatggcttc
     4681 accgagatca tgttgattaa cttggttcat agacaaacct ccttctgcta tttacttgtc
     4741 aaagaaccta ttgaacttat tttaagtaca tttttattgt acgcctgtat ttattttaag
     4801 tcaatatatt ttgacttttt ttaagtacac acgtatacta aatttaagaa cttatcctga
     4861 ggaggagttt tcatggagat agacaagcaa gcagtcggca atcgaattcg gcagattcgc
     4921 caagaattaa agttgagtat ggagaaattt ggcaaactaa ttggtgattt accgcgaagt
     4981 acagtcaata actgggaacg tgggatcaac cttccaaaaa ccgaaaccct tcaccaaatc
     5041 gcagagatag gtcatgtcac caatgaatat cttttgtatg gtgatcaaga gaatcaatac
     5101 attttagaaa tgctccaaaa aaaggcaggt aagcttgatc ctaagattga aggtctcata
     5161 gtagacgaaa tcaaacacgc tgaccttgtt agtgaaaaag aaatggatcg aatgattgaa
     5221 ttttttatta caaatctcat tccaccaact gaacaagacc agtttacttt ccaatgtatc
     5281 gatgaaaaaa agcagcttta tcttggttca actaattttg gtaaggaagc ccagctctat
     5341 ttgcaacatg ataatcaaaa tcatattctg cacatgatgc cctttacctt ctccaacttt
     5401 agtgtggatc gcatactcgt ctatttagca aatcaaaaat cctattccta ttttgggaaa
     5461 catttaccca aggaactagg tgagaaagcc atccttctat actcaattaa tcaattgacc
     5521 agcgatgtgc gaattgcccc gttggtctat tcagaagaaa ccgcttctta tcagtacaca
     5581 gaggacaatc aatatctact agaacagcaa tatctttatc taccattcgt gatggaagtc
     5641 gagaaagaac gcctgttaaa tgcagcgtat ccatcatcaa aatgaggtga caaaattgat
     5701 aagcacgtga caaagaaaat actgctagga cactccccaa tgggtagttg tcacagtttc
     5761 ctgttctttg ttagccccct gttagaaccg ggggctttat tcctgcagtc cggctacgcc
     5821 ttcctgcaat agacaaaact ccagatcggg taattcgctt gtggcgaaac ccgacctgga
     5881 gtttgtctct gtattttgaa ccctaaaaat taatcaaaca gactgatatt ctcataatca
     5941 gcgcgaaata agccattaag tacagttttc ctacctattt ttgataaccc gctggttagt
     6001 taatattcct tttccgagag agataaatga
//
LOCUS       DAHEYX010000162         5736 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHEYX010000162 DAHEYX010000000
VERSION     DAHEYX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.163"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            330..1253
                     /gene="truB"
                     /locus_tag="MB219_002160"
     CDS             330..1253
                     /gene="truB"
                     /locus_tag="MB219_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCC8427865.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1257..2198
                     /gene="ribF"
                     /locus_tag="MB219_002161"
     CDS             1257..2198
                     /gene="ribF"
                     /locus_tag="MB219_002161"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCC8427866.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2593..3378
                     /locus_tag="MB219_002162"
     CDS             2593..3378
                     /locus_tag="MB219_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8427867.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3391..4242
                     /locus_tag="MB219_002163"
     CDS             3391..4242
                     /locus_tag="MB219_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCC8427868.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4366..4818)
                     /locus_tag="MB219_002164"
     CDS             complement(4366..4818)
                     /locus_tag="MB219_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8427869.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5283..5651)
                     /locus_tag="MB219_002165"
     CDS             complement(5283..5651)
                     /locus_tag="MB219_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCC8427870.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1936 a          929 c         1162 g         1709 t
ORIGIN      
        1 gattaagaaa ctgaaaaaat aaataaaaaa aaacggaaaa aagactggaa gaatggacag
       61 tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa
      121 tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaaaacat
      181 aacatgattt ctatttacgt gagcatgaat tttgttacgt ttttattgcc gaaaaataaa
      241 aaccgttcat tatgatgtca aattcgtcag taattgatgc aatgtgttat aataattcag
      301 ttaaatcaaa aatgaaatgg agaaaagaca tggacgggtt attaacttta tggaaagaac
      361 gcggaatgac aagtcatgat tgcgtattca aattaagaaa aatcttacat acgaaaaaaa
      421 tcggacatgg gggtacgctt gacccagacg tagacggtgt gttgcccatt tgtattggaa
      481 aagccacaaa agttatcgaa tatttgacag attctgggaa aatctatgta ggcgagataa
      541 ctctaggttt cagtacaacg acggaagatc gatccggaga acgggtagaa acaaaaccag
      601 tagatatgtc tttatctgaa gaaaaaatag atgaagtgat ggcttcattt attggtgaga
      661 tcacccagat accaccaatg tactcagcgg taaaagtcaa cggtaagcgg ttatatgaat
      721 atgcccgcaa taatgaaaca gtggagcgtc ctgttagaaa ggctcagatt taccgtttta
      781 tccggacaag tgaattgaca agagacgaag tgacaggaac catttcttgg cgcttcgaag
      841 tagaatgcgg gaaaggaacc tatgtccgca cattagctgt agatacaggc aagaaacttg
      901 gttaccctgc gcacatgtct gatctgaccc gaacagcaag tgccggtatg tcccagaaag
      961 atgctctgac attagaagaa gtagctcaag ctgtgaaaaa tgggacaatc gatgattgtt
     1021 tgctgccaat agaaaaagga gtgaaacgat tcaaacgagt agatattact gatgaagtat
     1081 ggcagaaagt aaaaaacggt atgagactta attatactgt atttgcgctc ccagaaatgc
     1141 cgtcagaaga aattgctttg ttttataaag gcaaagttgt cagtatctat cagccaaacc
     1201 cgaaagaaaa aaacaaatta aaacctagta aagtattgag aaatgaggaa taacgaatga
     1261 atatcatcaa gatcagacat ccttaccaat cagaacaaat tccagaagac gatgtcgttt
     1321 tagtactagg attttttgac ggcgtgcata ttggacacca gaaagtcata aagacaggta
     1381 tggaaattgc acaaaaagaa ggattgaaat tagcattgat gactttcaat cagcatccat
     1441 cgattgtctt taaaaagatc gatcctagca gcgtgaaata tttgactagc cttaagcaaa
     1501 aagaagaaaa aatggccgct ttgggtatag attatttata tgaagttgat tttacttctt
     1561 cttttgctca tttagcaccg caggaatttg ttgatcaata catcgtaggg ttacatgcaa
     1621 aatatgctgt ttccggtttt gattatacgt atggaccaaa agacattgca gatgtcgctc
     1681 attttcctga atatgccaaa ggaagattca aaatcgtcac agtttctaaa gaagaagaac
     1741 aaggtgaaaa aatcagctct acccgtattc gaggattact agacagcgga caagtaaaag
     1801 aagcagcaaa actattaggt tgcacatatg aggtagaagg ggtagttgtc catggcgatg
     1861 cacgaggcag actactagga ttccctacag caaatgtcaa agtaaagagc acagttcatt
     1921 tgcctaaaga aggtgtttat gtatcggaaa taaaaattgg ggaaatttgg tatccagcca
     1981 tgggttcaat tggacataat gatacattcg gtgaaggacg ggagctgact gtagaattgt
     2041 atattttgga ctttaatcaa gatatctatg gtgaacatgt cgcagtacgc tggaatcaat
     2101 ttttaagaga tcaagtgaaa ttttctagtg tagaggaatt gattgaacaa ctcaaagagg
     2161 atgaacaagc aacagctgaa tatttttctc agcaataaaa cattatgcat aaaatctctt
     2221 gtttatgtat aactagcaat ttcaataaaa tgggtgataa ataagcccag gaaaggggct
     2281 gtgacaaaag tcttgtcaca gccttttttt ccaaataaat ggtttttcag aagtagcttc
     2341 ttcggaaata agccgaaatt tcacaaaaat ttgagaagta atttttgtaa attacttctt
     2401 atttcttgaa gctggccact tctgtcacaa cctctttctt tatagaaata aaaggacact
     2461 agcaattcat gaaaacgatt gctaaagcgt atcatttgct ttagactata tatcttggtg
     2521 tacactcact ttaagacgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
     2581 ggagtgggta ctatgtataa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
     2641 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
     2701 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
     2761 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
     2821 gatacggctt tagaaaattt tgggacactg gacgtttgga ttaataatgc tgggatggaa
     2881 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtatt aaaagtcaat
     2941 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaatgataaa
     3001 aaaggaaata tcatcaatat gtcttctgtt catgaacaaa tcccatggcc aacgtttgct
     3061 cattacgctg cttcaaaagg cggagtaaaa ctattcaccg aaacggtagc aatggagtac
     3121 gcaccaaagg gaatcagaat caatagtata ggacctggtg ctatccgtac acctattaat
     3181 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
     3241 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
     3301 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
     3361 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
     3421 gttgggtggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
     3481 atattaggaa ctggtattgg agccgtgctc gttggcttac ttgttcaatt attcgcttct
     3541 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attctgggtc
     3601 atcggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
     3661 atttcgactg cattacagtt agtcggaacc tcattgattg gtgtcttcgc ttttggagaa
     3721 tggtctggaa gtatgggaaa aatcattggt gcagcagcaa tcattcttct tgtatttggt
     3781 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatc
     3841 agtctattag catcaacaag tattggttat tgggtttaca gtgcattgcc caaactagtt
     3901 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
     3961 atttatgtga ttttccgaaa accaaccgca ttttctgaag acaagagttg gaaagcaaca
     4021 atagttggag ccatcttcag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
     4081 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
     4141 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
     4201 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
     4261 ctcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
     4321 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaagtga
     4381 aatatctgtc tgcatcaata atttttcatt gatccattga ctaaaattta cgacatgatt
     4441 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
     4501 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggatttg
     4561 attgattgct gtaaaatcac gatactgaag gttgaaacgc atcgtgattg gtgcatttag
     4621 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
     4681 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
     4741 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
     4801 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
     4861 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
     4921 gtcagaaaat tctaactttt ctttctctaa agaaattcgt tctctcctaa agtgatatta
     4981 tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt
     5041 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagtg
     5101 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
     5161 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
     5221 caaaacctgt ataagctgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
     5281 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
     5341 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
     5401 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
     5461 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
     5521 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
     5581 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tctgattgat gatcggctga
     5641 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
     5701 tagttatgta taaaaaacga acaaaacaca agtaat
//
LOCUS       DAHEYX010000163         5719 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHEYX010000163 DAHEYX010000000
VERSION     DAHEYX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.164"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            108..1004
                     /locus_tag="MB219_002166"
     CDS             108..1004
                     /locus_tag="MB219_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCC8427871.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1154..2095)
                     /locus_tag="MB219_002167"
     CDS             complement(1154..2095)
                     /locus_tag="MB219_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCC8427872.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2221..3342
                     /locus_tag="MB219_002168"
     CDS             2221..3342
                     /locus_tag="MB219_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCC8427873.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3600..5102
                     /gene="eat(A)"
                     /locus_tag="MB219_002169"
     CDS             3600..5102
                     /gene="eat(A)"
                     /locus_tag="MB219_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCC8427874.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5130..5696
                     /locus_tag="MB219_002170"
     CDS             5130..5696
                     /locus_tag="MB219_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCC8427875.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1875 a         1014 c         1208 g         1622 t
ORIGIN      
        1 atacaaatgt taaattttca tacaaatata ggaaacgttt tactggcttt ttactattta
       61 aacaggctat cataggaaaa agtgacagag aaagtgaggc ggaaaagatg aacaaaaatg
      121 acgtactgct agttgcaaaa aataaatcac tttatttttt gccttccatc cgctctttag
      181 atgtttggtc cggaaaagat ttactacagt cggaattcat taaatcagct agacaacagt
      241 tggcaaatta tcagctgatc gtttttttag attatggatt tgatatggag atggctcgac
      301 gcgtgcgccc atttaccaat gcaaaaattg tgttattttt ttggaatcac ttcaaagcag
      361 aacacgaggc gatgctgaag caggcacaag atgaaccggc aatcgatgag atttatcatt
      421 ttgatttttt ggaagcaaaa gaacttgggc tgaaacataa tagttcattt tattcgaaac
      481 atatgaattt ttcttctagc gaaccaaaga cggatctatt ttttggtgca acgaataatg
      541 ggagaaaaga acgagcagaa agctataagc aggaatttat aaaaagagat gttacgacca
      601 actatttcat tcttccacat cgagggaatg aacaacctgg atatcttact tatgaagagt
      661 acctgaaaaa aacaggggaa tcgcgcggga ttctggaact gttgcgggag ggtcagcaag
      721 gagtgacttt gcggacattt gaatccatgt attttcaaaa gaaactagtg acggataatc
      781 aagcgattgc tcattatcat ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg
      841 atttagatga attaccggaa tttctgcata caccatttaa gccaatccta tcagagctat
      901 tggaatttta tgatgcagaa aattgggcaa aacgatttat cactaacgat tccactattt
      961 atgagcaata cgaatatgac ctctcctctg ttgaaaacaa ataaaaaacc tacattttga
     1021 agagattatg tactgaaaca aacaagcaca attcttatgg catcatgaga gttgtgtttt
     1081 ctttttgatc ttttattggg cttaaaaaaa gagaaccagc tgctgattca gctgattctc
     1141 atcagagagc cttttattct gcgccaagga ttccttcttt tgcatggact aattgggtca
     1201 aaaaggcaca atatggtgta gcaatatcat atttttgtcc ttttcgccaa atggcaccat
     1261 tgatgtagtc gatttcagtc aaacgatggt ttttgatcag gtcttggtac atagatgggt
     1321 aatgcaaccc gatgttgtca ggatcataag tagaagcaat atgttggtac acttcttctt
     1381 gatctagtat gattccttct tttgctgcga tagaagcaaa ttcagatacg attgttcgaa
     1441 ccatgctctc tgccgctttt tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc
     1501 ataaaccatt caatgttcca tttacacaag cttttcgcca aatggaataa cgaacgttat
     1561 ggctatagat cggatttaaa tttgcttctt gaaagacttc tacaactttt tttgtaaatg
     1621 cttcgccttc tggttccaag ttctctagtt cgatttctcc atcacctaac aatgtgactt
     1681 ttccgggacc agctagacca gctgtccaca tcgtgatccc aaataagata tttttctttg
     1741 gcacatattt ttccagtaca tcttcatgac ctaatccatt tagtaaacac aaaacataag
     1801 tattgtcagt aataatggat tggatcgaac aaaacatatc atctaattga ttggcttttg
     1861 ttaatgctac gatcaagtcc acgtgttcgt tgctttcgat gatttcttct ggtgaataga
     1921 ttggcagttt tgcgaccact tctttaccgt taaagtcagc aatcaaccca tttgtacgaa
     1981 tggcttcaat gtgagcaggc caacgatcga tcaagaggac ctcatttccg ctttgatgta
     2041 gcatcaatcc gattcggctg cccattgctc cggccccagc tattgcgatt ttcatgtttt
     2101 ttctctcctt tattcaatga gttccctttt agtatataga aaaatggaag aaaatagcat
     2161 tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg agggatacag
     2221 atggatattt tggcggcaat tgattcattt aaaggaagtg cttcttcatt tgaactaaat
     2281 caggcagttc taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca
     2341 gatggcggag aaaatactgc agaagcaatc tattacgcgc tcaggggaac atggcgtacc
     2401 attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt gacaacttgg
     2461 caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat
     2521 aaaccaacgg atcaaactat ccgacttgct tccagttatt ccttaggaga acttattaaa
     2581 gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc
     2641 gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga
     2701 aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa
     2761 gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct
     2821 tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa
     2881 gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca
     2941 ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt
     3001 tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat
     3061 gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac
     3121 ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga
     3181 gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt
     3241 ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta
     3301 ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct
     3361 ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa
     3421 gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag
     3481 attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt
     3541 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa
     3601 tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat
     3661 ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg
     3721 gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag
     3781 taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa
     3841 cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc
     3901 aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag
     3961 agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg
     4021 atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa
     4081 agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg
     4141 accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct
     4201 atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa
     4261 aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag
     4321 aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata
     4381 aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc
     4441 ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt
     4501 ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc
     4561 aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc
     4621 gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg
     4681 gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt
     4741 atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc
     4801 aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt
     4861 ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat
     4921 ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct
     4981 tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg
     5041 aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct
     5101 aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag
     5161 agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag
     5221 tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag
     5281 cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac
     5341 gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg
     5401 aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact
     5461 ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg
     5521 tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc
     5581 tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta
     5641 atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat
     5701 gtttttgtgt tatttaaat
//
LOCUS       DAHEYX010000164         5710 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHEYX010000164 DAHEYX010000000
VERSION     DAHEYX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.165"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(40..342)
                     /locus_tag="MB219_002171"
     CDS             complement(40..342)
                     /locus_tag="MB219_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427876.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(562..928)
                     /gene="ssrA"
                     /locus_tag="MB219_002172"
     tmRNA           complement(562..928)
                     /gene="ssrA"
                     /locus_tag="MB219_002172"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1099..1872)
                     /locus_tag="MB219_002173"
     CDS             complement(1099..1872)
                     /locus_tag="MB219_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8427877.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2016..2366
                     /locus_tag="MB219_002174"
     CDS             2016..2366
                     /locus_tag="MB219_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCC8427878.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2347..3063
                     /locus_tag="MB219_002175"
     CDS             2347..3063
                     /locus_tag="MB219_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCC8427879.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3063..4511
                     /locus_tag="MB219_002176"
     CDS             3063..4511
                     /locus_tag="MB219_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCC8427880.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4581..5090)
                     /locus_tag="MB219_002177"
     CDS             complement(4581..5090)
                     /locus_tag="MB219_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427881.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5080..>5710)
                     /locus_tag="MB219_002178"
     CDS             complement(5080..>5710)
                     /locus_tag="MB219_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427882.1"
                     /translation="IACLTLYHLQSALANQVQDRLSFIGASASTELLPALELASRFYI
                     KGIFSLFFLFAFLFIFFFYYSVRQNKQELINWHLTGLSKTKLFLFIFWQLFFPLFLCC
                     ILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQQTFFTIDF
                     QNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1923 a         1010 c         1117 g         1660 t
ORIGIN      
        1 taaaatccgt tccgggctac cttttttagg tagcctactt taatctttat acctttctgg
       61 atcaacgaaa gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac
      121 atcagtcacg atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg
      181 aattttaaag tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc
      241 taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg
      301 atcaaattta aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag
      361 agtatacgaa ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac
      421 atatatatta aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa
      481 cgaaaatctg cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta
      541 gcgaaatctg ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca
      601 tcgccactta aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc
      661 cgagtgacag gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa
      721 agttgaaacg tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga
      781 ggatctacgc taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag
      841 ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga
      901 ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat
      961 acaaaattag tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt
     1021 atttatataa taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg
     1081 tagcttcaaa aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc
     1141 ttctgtattt ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc
     1201 catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa
     1261 tccaaaagta ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc
     1321 ttttgagatt cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag
     1381 ctcaggaaaa tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt
     1441 tactgtgttt tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc
     1501 gtaagcattg atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta
     1561 agaaagttca tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga
     1621 aaatgtttga gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac
     1681 cgcagaagta atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt
     1741 agcgatgacg gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg
     1801 tgtgattttt gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg
     1861 ataatttgac acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg
     1921 aacaaaagac aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag
     1981 agtatactat aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct
     2041 tattcttaat aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt
     2101 atgggtatca ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga
     2161 caagcggaaa ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa
     2221 agacatctgg gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta
     2281 tgcctatttt tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga
     2341 acgataatga atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg
     2401 tttttcttaa atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat
     2461 gcctttttag aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca
     2521 ctggacggta tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc
     2581 atgctgacag ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat
     2641 gattatgtca caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat
     2701 cgccgtagtg agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat
     2761 atcgagacta gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg
     2821 ccaattgaag gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca
     2881 cgtcaagtat tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt
     2941 gatgaacgga cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga
     3001 ccacaagtca tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc
     3061 taatgcgtta tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat
     3121 tgatcgttgg agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc
     3181 agcaactgtt tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc
     3241 tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac
     3301 tgacagaaca aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga
     3361 ctaatacagc tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg
     3421 gacgtcaaga aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt
     3481 acgcacttgt gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac
     3541 ctgcgcgaaa tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta
     3601 ttttttccag tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc
     3661 gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc
     3721 tccccgtaca tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga
     3781 attcattgaa agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta
     3841 tggcagatgc ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag
     3901 ggttagaata caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa
     3961 atgaaacaga gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta
     4021 cgaatcaaat cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg
     4081 ttgcacagtt agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg
     4141 atgatattga tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc
     4201 aaaacgcgat acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc
     4261 ttgaaacgat catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa
     4321 gtatctggga tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat
     4381 caggattagg tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag
     4441 tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca
     4501 aaaaagagta aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt
     4561 tttactataa aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga
     4621 gcaatagtag catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa
     4681 gaaagcaagt ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca
     4741 ataacaatat tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca
     4801 aaagacgtca actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct
     4861 tgataggcca aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct
     4921 ttaagattca gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt
     4981 tttgcaacaa gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga
     5041 cacaaaataa cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg
     5101 cctcctactt tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca
     5161 gcatccactg agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag
     5221 atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct
     5281 taccgaagtc aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt
     5341 gatcctttga agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa
     5401 gatacaacaa agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt
     5461 tttagaaaga ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata
     5521 gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat
     5581 ataaaatcgg ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat
     5641 aaatgaaagc cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag
     5701 gcatgctatc
//
LOCUS       DAHEYX010000165         5655 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHEYX010000165 DAHEYX010000000
VERSION     DAHEYX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.166"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            832..1566
                     /locus_tag="MB219_002179"
     CDS             832..1566
                     /locus_tag="MB219_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCC8427883.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="MB219_002180"
     CDS             1582..2751
                     /locus_tag="MB219_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCC8427884.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="MB219_002181"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="MB219_002181"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCC8427885.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="MB219_002182"
     CDS             3841..4767
                     /locus_tag="MB219_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCC8427886.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..>5655
                     /locus_tag="MB219_002183"
     CDS             4786..>5655
                     /locus_tag="MB219_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8427887.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAIL"
BASE COUNT         1882 a          931 c         1179 g         1663 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt aaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca tttta
//
LOCUS       DAHEYX010000166         5608 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHEYX010000166 DAHEYX010000000
VERSION     DAHEYX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.167"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(228..935)
                     /locus_tag="MB219_002184"
     CDS             complement(228..935)
                     /locus_tag="MB219_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427888.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1000..1836)
                     /locus_tag="MB219_002185"
     CDS             complement(1000..1836)
                     /locus_tag="MB219_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8427889.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1854..3263)
                     /locus_tag="MB219_002186"
     CDS             complement(1854..3263)
                     /locus_tag="MB219_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCC8427890.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3277..5100)
                     /locus_tag="MB219_002187"
     CDS             complement(3277..5100)
                     /locus_tag="MB219_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8427891.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1701 a         1233 c          927 g         1747 t
ORIGIN      
        1 tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga aaaaaaatac
       61 ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata acaacggggt
      121 agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt aaatacttcg
      181 ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatcttttta cccattttca
      241 agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact gatacctgtt
      301 agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt atcagtattt
      361 atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc tgtgtatgtt
      421 tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc tgtctcatta
      481 ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc tgtctcatta
      541 ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt agtgtgcata
      601 gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc aatagagact
      661 tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag cattttgttg
      721 tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg gaatacagct
      781 ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc tccaagtagc
      841 tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt agaggtcgaa
      901 acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt agcacaacca
      961 acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc ttgaaatatg
     1021 cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt cttgaacgta
     1081 ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca ctgaagccag
     1141 caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa taaaaaactg
     1201 taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt tttcgggaaa
     1261 cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca tggtttcttc
     1321 catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc cagcctcctc
     1381 ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc agccgatctc
     1441 aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa aaacaatgcc
     1501 tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa atagactttt
     1561 attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga cgcaaataat
     1621 tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag catcagaaag
     1681 tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc ctttctttag
     1741 ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat ctaagcaaag
     1801 aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc tctctaagct
     1861 aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt tattcgtttt
     1921 aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg cttattgaag
     1981 ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa aacatctaca
     2041 ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc aattcgatcc
     2101 tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc attttcagta
     2161 attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg tagtccagta
     2221 gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc tgcccggtag
     2281 acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac atctgatccg
     2341 tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat aaaatcgggt
     2401 ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa tccacgatca
     2461 ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc taagaaagac
     2521 cagcctctca atgtttccca attatgggct gcaattgcat cttctggact ggaggtagct
     2581 tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt caactgatga
     2641 tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg aaagagttct
     2701 ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg aaggatcatc
     2761 gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc agtaagcaat
     2821 acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc gccctgctcc
     2881 tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat tccctgattc
     2941 aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt tttattattt
     3001 tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa gcctaattct
     3061 ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac ttcaaaatta
     3121 gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc atcctcctga
     3181 taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt ctctggaaat
     3241 gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca tcaatggttc
     3301 gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac tgttggtcac
     3361 taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat cggcgttgat
     3421 taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa agggttcccc
     3481 ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt ctttagaacg
     3541 tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa caggtgaata
     3601 aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct tagaaaagac
     3661 agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt caacaatgac
     3721 tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc caattagata
     3781 agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt gaacaatatt
     3841 cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa caaaggcata
     3901 tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa tcatggaagc
     3961 aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag taattcccat
     4021 taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt tagttttcgc
     4081 ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc ctggcccatt
     4141 gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca ttgcccaagc
     4201 tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa ttaaccaagc
     4261 tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca acgttccaaa
     4321 cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca tcggcttagt
     4381 aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat aggacatgat
     4441 ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta ataccggaat
     4501 tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg gatgaagcaa
     4561 aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg ctgccccata
     4621 ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca aaatgatgta
     4681 agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa tggcttttac
     4741 catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa tgacactaat
     4801 caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa ttgaagttcc
     4861 cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca ccacttggta
     4921 ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg ctttcgtatc
     4981 ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg ttaagctgac
     5041 cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc tgtaatccat
     5101 tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca atcaacattg
     5161 aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata acgttatttt
     5221 gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt aattttttat
     5281 cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta gggtatatct
     5341 gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta aaaccattcc
     5401 tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat ataacggatc
     5461 acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag taaaggatgg
     5521 aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt tggtaaaaat
     5581 cagtaagcaa tagccaattt caatcatt
//
LOCUS       DAHEYX010000167         5605 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHEYX010000167 DAHEYX010000000
VERSION     DAHEYX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.168"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(183..581)
                     /locus_tag="MB219_002188"
     CDS             complement(183..581)
                     /locus_tag="MB219_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCC8427892.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(578..814)
                     /locus_tag="MB219_002189"
     CDS             complement(578..814)
                     /locus_tag="MB219_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCC8427893.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(974..1258)
                     /locus_tag="MB219_002190"
     CDS             complement(974..1258)
                     /locus_tag="MB219_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427894.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(1429..3657)
                     /gene="topB"
                     /locus_tag="MB219_002191"
     CDS             complement(1429..3657)
                     /gene="topB"
                     /locus_tag="MB219_002191"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCC8427895.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(3831..4241)
                     /locus_tag="MB219_002192"
     CDS             complement(3831..4241)
                     /locus_tag="MB219_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCC8427896.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(4358..5521)
                     /locus_tag="MB219_002193"
     CDS             complement(4358..5521)
                     /locus_tag="MB219_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCC8427897.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
BASE COUNT         1502 a         1168 c          908 g         2027 t
ORIGIN      
        1 gttctaaacc actatttcct actattcact cttttgtatt ccttttaaat caattatttc
       61 aaaattaatc catattttcc atatttttat gaaaaaacta ttttgtatga ttaaacataa
      121 aaaaatcagc atattcctta atgacatgct gatctaattg agaaataaag tttttaattc
      181 gttcatttta ttgcggtttg tcgtaaataa ctggttatct tggactttag tgaatcgaaa
      241 tccaatgaac tagtggtaat cattatgata aaatcaacag cttcttgacg agaaaaagca
      301 actggacaat tattcaattg aagaaacaac aacatggcaa caagagcagt tcgtttattg
      361 ccattatgaa aaggatgttt tttagctaaa ttaattgcta aaatcgccgc tttttcatag
      421 atttcaggat agagttcttg attgaatact gcttgtgaag gctgtttgac ggccatatct
      481 aatgcatttg catcttttac accgatcatt tcgcctggcg agtgacgtaa aataactttc
      541 gcgttgattt tgataatttc tttagtcgaa agatacctca taaatcaacc aaatctttta
      601 acgcgtcttc atattgattc atgatcgaat caactaatga atcaatatca ggttcttctt
      661 gtgctttgat cattcgtaca gaaccatcag attgaaaaac ataactgatt gcgtcacctg
      721 tctgcacacc catgaattct tttacttctg gtgggatagt tgttacatcg ctattgccag
      781 attttctaat tttatacttt ttcatttttt tcatatttcg ccaccctcta taccattctc
      841 attctatagt aagtacatta tatccaagta tatactttta atcaatgtat atacttttat
      901 tttgcttttt cttacgaaaa aatgacaaag aaaaaagttt gtgacatgac ttttgtcaca
      961 aactctcgta ttcttaacca aaatattgat gaaatacttg tgtaataact ggtagaaaat
     1021 aaataatgat catagccgct accaccacca acaattgttt tcgctgtttc tttcgcttta
     1081 gcatgcttgt cgttttatga aaaaaataag tagcgctttt agctcctgca gtcaaagtcg
     1141 ataaaatcaa cgttggtgaa tatgctgttt gattgtgcgt caatgacaac aacttcgtcc
     1201 cgtaatcagg atactttaga aatgcttggt acacagccac aatcgtgatt gctcccataa
     1261 gataatttaa aaatgaatgc acttttttca aataaaaaaa ctccttgcta tctatataaa
     1321 atcagcctac cccaacgttt ttgcttgatt cactgatatt ttttagtttt attggtattt
     1381 atattctcac ttttcggaat aaatattccg ctatttcatt gttttcattt actctacttc
     1441 gttttcatta ttttttcttg atgtttcttt gtttccatca actgcttttg ttccactgtt
     1501 ttcgatccat ggaaaagaaa aaccgattgt catatcttct tttaatacta aaactgcatc
     1561 gaatttccct tttttaccag taaaaccttt gacaacttta cttgtctttc cttttgaaag
     1621 caaatctctg agattcgttt tagtcagctt ctttttagct actgttggcc acaatttgaa
     1681 gtcgcattcc ggattcaagc atgtagcgat tttaggataa agcatgacta ttcccttttt
     1741 acattttgga catcttccta attgatcgtt tttcttctcc atcgctttta tctgacgtgt
     1801 cccgctaatc tcatctgaaa agtctacctt gtcgatttga ttaggaactt ctgccaataa
     1861 atgtaggatg aattttttga tattctctaa aaaaacagat ggtgtcccct cttgtttacc
     1921 aatttttctc aagtaccctt cccagcgcgc agtcatttct gcgctcgtca gtaatggttc
     1981 attggctact gccttacaaa gagtgattcc ttttggcgta acagttattt cgtttttatt
     2041 gacttcgatg taatgcttct gcttaagatt ttcaatgata ttggctcgtg tagcttctgt
     2101 ccctattcct tctacttctt tgagaatttc ttgcgcttca tcattatcca gcgtttttcc
     2161 cgcagttttc attgctgtga tcaacgttcc ttcggtatag ggttttggag gttgtgtttc
     2221 tttctccata cttttcagtt ctacttctgc ttcttcattt tctacgatag gaggaagagt
     2281 ctgagcttct ttcttttttg acttgatgcg taagatttcc tgccaacctt gctgtaatgg
     2341 gatctttccg atgcttttta gctttaattg ctgtacgccc gtcattacgg tcgtctcttc
     2401 gtagatataa tctgcagcaa acatagccac agtagtgcgc aaaacttgca aatagattgc
     2461 ttgctgtaat ttggaaagct tcgcaaagcg tgcaggagtc ggaatcgtct ttgtcggaat
     2521 aattgcatga tgttcttgaa cttttttcgc atcgacatat cgtttctttg ctttgatctc
     2581 agttgtctgt acttcttcta ttcctaaaaa ttttttataa ttttgcagat gtttaaccag
     2641 atactgatgc tcattttctg taatgtaaaa ggaatccgtt cttggatagc ttagaaactt
     2701 gctttcgtac aatccttgag cagcatcaag tgtagctttg gcacttgctt tcattagctg
     2761 attcatcttt gtctgcaaac tagatagcga aaataaacgt ggactatgca acctcttttc
     2821 ctttttcgta acttcaagaa tccgtccgtt ttgcttacct aaatgcgctc caagtttttc
     2881 tatttccttc gttaactctt cttgggtttt aaatgctttt tttggtacga tttttccaga
     2941 aaactgccct ttgtttgtcg taattaaacc ttctccttca aagtacgttt cttttttgaa
     3001 gtgtttgatt tcttcttgca gctgataaat catatagagt gtcggtgttt gtacacgacc
     3061 aagagaaaaa ctgccatcaa tcccttttcg ttgaagtagc aagctatata atggacttcc
     3121 attcattcca atcagccaat cagcgttctg ccttgcttgc gcttctttat attttggaaa
     3181 atagttcttt ccaggttgga gattcttaaa accttcatag attgcttctt tttcaagaga
     3241 attgatccat aaacgtaaat agttcttttc cttagaaaat gcgtttgctt gtcgaataat
     3301 cgaccacgcg atggcttcac cttctcgatc accatctgtt gcaataatga tggtatcggc
     3361 tgactgcaat tctcgtttta cgatcgaaaa ttgtgcacgt ttgtccattg gaacttcgta
     3421 acggaaggtt tctgggaaaa taggcaaatt ttctaaagac cattttgccc aacgctcttc
     3481 ataagctcct ggagcaacca tatctaccaa atgtccaaaa ccataagtga tggttacttc
     3541 accagcaaac aatggatcgc tgatttcata atacccttca tgccgctttt gctgttggaa
     3601 ggtttcggca taggcttttg cttgtgaagg tttttcagct agtatcaccg tcttcatctt
     3661 ctgacctccg ttacttaatt ttttgcggac ttctctatta tggaacatat tttcgcatct
     3721 ggcaataaaa aaaagatacc gtatagtcga aatagacaca aaaacatcaa gcaaacaaac
     3781 gatgatccgt ttgctgcttg atgtttccag aaaatattca tctttagctg tcactcttgg
     3841 ttaatctgcc agttgatgcc aaatcgatca gttaccatcc catacaactg agcccacgga
     3901 acagcatcca gtggcatctc aatcttacca ccctcagcta agctttcaaa taggcgcttc
     3961 gtttcttctt catttcttgt ctggatgact aaagaaacat tatttcccac aactaattca
     4021 ggaccaattc cttctggcgt atctgcaaac ataactaatg tatcataaat gttcagccgt
     4081 gcattcgtga ttagattttt tgcttcttct ggcaagttca tttgttctat tccaggcatt
     4141 tcaccgtatg tggtgatacc tgatgcttca gtctgaaaaa ctttttcata aaaagcgatg
     4201 gcttcacgcg actcattttt gaagttcaga tagatatcca atgtaaacag ctcctctctt
     4261 ggttaaatct accataagaa agcgtttttt acgagtgaat cgtttgattg attcatgaca
     4321 aaagccttgc gcagcttctt cttgatcttt tttatgatca tttattcaaa aaatctgtaa
     4381 tagcagattg attcatttga ttatcaattt caagaatact gccagcataa tcactttgcc
     4441 cataattcca cgaaccgtct actggcacac ttattcgatc aatgccacca gctcctcgtg
     4501 caatcgaaaa gaggtttttc gtcaagaacc aagcagtgac atctgtggat gtatatccta
     4561 aagctttacc agctgcataa ggtgcacgga tcagattcaa tggattcttc aattggctga
     4621 aaatagcgtt cattacttgc tgttggcgtc tgacacggcc aaagtctcct tgttcatcca
     4681 ttcggaaacg tgcgtactgc aaaagaacat gtccatccat cttctggacc ccttttttaa
     4741 tatctacacc atccagattc agatcttttt ctgcatcgat cttcactccg ttcatgaata
     4801 aggcatcgat cactttttca aacgatttga aatcgacttt cgcatagtat tgacaatcga
     4861 ttccaaaatt ttctactaaa gtttgacgca ctagatcggc tcctccgtat gcataagctg
     4921 aattgatctt attttgtcct acccctggga tcgtaacgaa gctgtcacgc ataaaagaaa
     4981 tcagtttcgg cttatgggaa ggaccatcca actgtaagac catgatcgta tcggctcgcc
     5041 cggcatcttc tccgcgtgta tcacttccaa ggatcaaaat attatgcgca ccattcgtcg
     5101 ctttttgccc atgaaaggtt tctgaagtcg ctgcctctaa gcttgtgtca tgttcagcta
     5161 catatttacc aataaaaaat gagatacctg aataaaggat caaaacaagc aaaatcgata
     5221 gaatgatttt tttccacgat cgctttcttc gaggttttaa cggtttgttc ttcttttgcc
     5281 gattgaaaaa ttttttattt ttttgttgtt cttctgctct tggtgcatct ctgtatatct
     5341 gttgctcgta ttggttcaaa tgttcaaatg aatgctgctc tctttcataa atacgggtag
     5401 catcttcctc attttccata cgcgttcgtt ttcgttctct tctagcaaaa atactccccg
     5461 tatcccgctt cagaggtttc gccttcttat ggatgtcctt atagcgatcc atccgactca
     5521 ttgtggttcc tcctcgtttc ttactgataa ataaaggata cgataaccaa tcgacaaaag
     5581 cctatcattt tttgataatt taata
//
LOCUS       DAHEYX010000168         5586 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHEYX010000168 DAHEYX010000000
VERSION     DAHEYX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.169"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..242)
                     /locus_tag="MB219_002194"
     CDS             complement(<1..242)
                     /locus_tag="MB219_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427898.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRK"
     gene            complement(229..756)
                     /locus_tag="MB219_002195"
     CDS             complement(229..756)
                     /locus_tag="MB219_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427899.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(779..2116)
                     /locus_tag="MB219_002196"
     CDS             complement(779..2116)
                     /locus_tag="MB219_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCC8427900.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2236..2715
                     /locus_tag="MB219_002197"
     CDS             2236..2715
                     /locus_tag="MB219_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427901.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(2830..3062)
                     /locus_tag="MB219_002198"
                     /pseudo
     CDS             complement(2830..3062)
                     /locus_tag="MB219_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3065..3382)
                     /locus_tag="MB219_002199"
     CDS             complement(3065..3382)
                     /locus_tag="MB219_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427902.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(3493..3675)
                     /locus_tag="MB219_002200"
     CDS             complement(3493..3675)
                     /locus_tag="MB219_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427903.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(3712..4512)
                     /locus_tag="MB219_002201"
     CDS             complement(3712..4512)
                     /locus_tag="MB219_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427904.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(4649..5068)
                     /locus_tag="MB219_002202"
                     /pseudo
     CDS             complement(4649..5068)
                     /locus_tag="MB219_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5064..5372)
                     /locus_tag="MB219_002203"
     CDS             complement(5064..5372)
                     /locus_tag="MB219_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427905.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
BASE COUNT         1680 a         1196 c          693 g         2017 t
ORIGIN      
        1 aatttcctac tttgttcctt aatttcagca tttcccaatt ctttgtattg agcttcctgc
       61 atagctttca ctttctggtt attcttgact acttcgattt gtttactttt aaggtcttcc
      121 aattctttga cattattatc ataagcattt ttcttttgaa atagtaccac acaacaaagg
      181 atcacaatta tggaaagtat tccgatcgcc tttttagcag tagtgatttc atgattttgc
      241 ataaaaagca cccccagtca ctttcttatc attatttagt gtgatggtta atgtggtatc
      301 atacactttt tttccattgt attgaacttc aaaatcggtc aatgcttcat acaacccact
      361 actagatgtc ttaattccaa agtttgttag cttaaaagta aaaggcttat agtactcgat
      421 tttttctttc tcactactct tatcagtaat tggtggttta cctgttacaa taaatagttt
      481 tctcaaaaat tcttcacctg attgatctgc aaacttattt tgaactgatc cattcaacat
      541 ttcttgaata tcattttcta aatctttccg taatccatcc acctcatttg gtttaacctc
      601 tttaaagaat gtcagcttat cttcaatatg cacactgtgt ttttcagctt ctatgtcctg
      661 attctgctta ttgaagaata ggattccaac aagcagtgct aataacatac cagaaagcaa
      721 agaaatgata attgtatctt tcactttact attcatctat atcgtccttc attccaattt
      781 atttttcgat ttcaaattta gcttttccta caccgctaaa gaaatgcaca ttatctgatc
      841 cgtccactcc atcaatcaac gttaccgaaa ctccccttgt tggagcttgg gttggtggaa
      901 cattaaagtt agcacataga aatgatccat cttcaaacac cgctataact acaccagtat
      961 gccctgcatc tccaacagcc cataaccatc catccgaagc actaaaccca taacctacga
     1021 ttggtttgtt cgttattttt gctcctgccg atttgtagct ttcccataca taaccaccat
     1081 ttccagtagt cggttgattc ccatcgtata attggctcat ataagcccac gtcaactctg
     1141 tacactgacc ttgatatatt ccgttaaaag gtgaagttga ccaatctggt tctttgaatt
     1201 ttggtagcgt gatcgctttc ccatattttt tcaaagcttc ttcataagtc atattcgcta
     1261 ttacttgacc ttttttaccg ttaatttttc cttctttccc aattgatcca gaaccagaat
     1321 tagcgtcatc cgtagttgca caggcatctt ctactgttcc tgatgctgaa cttgatgaat
     1381 tattcgatcc atctccggta cttgaacctc cggatatttt attgatctta ctttcatctt
     1441 ttgcaccgtc catacccttg cttttcatgt acttccagat ttcttttgca ttttcaccac
     1501 gtacagctat tacagctgat tcggaatcag ctgaaatttc ccagttttta tgaaaagtta
     1561 ctgcattttc tacgacatca tcagaagcat ttaaagcata atcttttaat attcctacga
     1621 atcccccatc acctttgaac ataaagtcca attgggcttc tgtagtccac caagaaccgc
     1681 cattattttt cttagaccaa tcaactaatg ctgtatgtcg cccagctgtc cattgaccat
     1741 agccaatacc catcagacca tcagtaaatt gtttgatgaa gtcaacttct ctctgtccat
     1801 ctccgtaata cccttctact gctcgaggat caatacctga ttcttgtttc cagttaccta
     1861 aaatacctgc aatactagca cctgataagc catattctcc attcatgtat ttgtaaatat
     1921 cttcaatatt tttggctaca tcacctgtta cagcgccact tccagtatcg tttccatcgc
     1981 tatctgttcc tgacccacta ccttcagtag tacagttaaa ttgtgcatca taagtagcta
     2041 tcccatcagc atgtatcact tgcggattta ttgtgattac actcattgca atccctgtaa
     2101 attgcaccat taacataagc aatgtggcaa tctttctctt agattttttc aataactacg
     2161 cccccaaaaa gtgtactttt ttatgttatt atgataatat aataacataa attaatagaa
     2221 aggaaaagct aataaatgac tgataaaaca agtgatttac ctaaactagc tccaaaaaca
     2281 tctcgagtaa agagcaaaaa agatggaaaa acaaaaaatg ctgtaaagaa aaagtcagta
     2341 aaagaaacat cctcgttact acttgatatt ccaatgagtt cttttgatga caatacggac
     2401 gaaacttctg ttaaaattgc aactcacgtt ggaatgaaca atcaaaatga ttctttttac
     2461 aacttgttaa acagcctaag agagttggaa gataaaaaaa atcaggtaat cgcatcaaaa
     2521 gctcctgatc tcacaaccga tttggatgct gaaattatcc aaatcaaaca agatgagcga
     2581 tcagttaaat cgctactgaa aacctacttt gatggttttg ataaagaact agctccttat
     2641 agccaagcaa tcctaactaa accaatacaa gctccacata tttcaatttc cctacaaaaa
     2701 aagagtgatg attaaacaat cattactctt ttttagattt ctggtaagcg ctgatcgcaa
     2761 aaaccatcaa caatattgat tcaaaaaata tgttcattcg tgtctctttt ttccattata
     2821 gatttcccac tattccatta aatcgaaagt cacaccgatt actacaaaga ttaacgtgac
     2881 cacattcaaa aacattaaaa gatatgctga taatctcatc atttttttag ttcacttact
     2941 ggtaatctaa acatttgaac gattgcactg gaaataccat aattagcaat gacaaacaaa
     3001 aaatacgcaa taatttcact tacacctaga tagatcagta aaataaataa gtcacttaac
     3061 attgttagtt tcctcccagt atatgatttt catttttggg aagtctttta taatccaatt
     3121 cctcaaaaaa aggaaccata gtgtcactaa tataggtttt agtttcaggt accgccaaaa
     3181 ttccccaatc aagaatttct tcccatgata catcatcaaa ttctaaaata ttatgatcaa
     3241 ctgacctatt accaagttca tatccaccaa agatcaccat attatcaatc atgtatagaa
     3301 tttcatcagg cgaaccatga gttaatgttt cgtcctcttc aagcagttcg tttaatcttt
     3361 gtttgtattc ctctcttttc atacttttat cccttctgac tcattttttt gaattttaac
     3421 atttttaacc caagcatgac tatctttaca tgctattaat ttttatctcc attttgatat
     3481 ttctgttttt ttctactcac caatcaaccg taaatcctca atattctcac cttcaagcga
     3541 atatgaatat acttcgccgt aaatattttc tataactacg acttcttctt caaagtctat
     3601 ggactgcaca tcaaaccatt ctcctccgcc aaattcgtct atgattagta cctgcaatat
     3661 tttatttact gccatttcaa tttcccctta taccattcta tgtcttccaa actatttttc
     3721 ttctttccat accaaggttt cacgtatctc aaaattttct ttaaataagt gttccggtac
     3781 ttttataatt aactcttttc ctacttttat cagatagtct ttttcaccta atgagatttg
     3841 accagtcggt aactgtatag aaactctttt agttttatta aattttttag ttttcaatac
     3901 agtatacggt ctacagaatt tctctaagcg ttcaaatctt ttactatcgt attggaaata
     3961 agctttagga acagataaag tacaaaaact atcggtaaca gcacttatgt cttttttaga
     4021 aattatccca actttagaac ttctaaggaa agttgctaat ttcttatatc caccatgact
     4081 tgtttcaata tagatgctat tgtattggct gtcgtctatc tcatttattg tttcaacctt
     4141 tttcctgaca ggtgtttcaa cgagttctcg aacctcttct tcactgtacg ctaatacaat
     4201 taactcttcc ccatttacca tctttagaac atacgcttgc ttcttttttc ttggcttatt
     4261 ttgagattca acataaccag acatggaatc tttatacttc caagcatgta cttttttctc
     4321 taatttctgt atctcttttt tcagctgtag atattcagac attttttcat tgtaacgttc
     4381 gcaatataaa caatattcat cttttccaaa atcatgatca agtccttctt caatatgttt
     4441 attcaacaaa ccggaaaaat actgatcata ctgcttcttt cgtaacttca gctctcgtaa
     4501 atcttcagac atttcatttt tcctttccaa ttattggtgc catttcacaa tttgggattg
     4561 actaaacacc ataattaatt tgtaagctaa cacaattaca actgttggta attgtgttag
     4621 tctccatcaa cttgtctggt ggcttttttt attgcacaaa tttcatagct ctcaaaaagc
     4681 gcttggcttg aaaaggtgtt attctacgaa ccttattcgc caatttccaa tctgataaag
     4741 caatttgcca ttcgtgcatt tcaaaatccg gcatgatttc tttgatatac tgtagctctt
     4801 tcttttctct ttcttttaag aaacggctca tcgagtttaa cattggaact aaggatgcgt
     4861 aggtacttga ataacttcca atgcgtttcg tagtaattac ataatcaata aaaacactat
     4921 taagaaaggt caagtttata aactctgatt catacactct aaaatttgca gttaatcgct
     4981 tcgccttgtc accccaatag tcacttttgt tgtaggcttt ttcccctcga atatagcact
     5041 ctgtttctct ttcgtaataa tagtcatact tttttcatca tcaaatctga gagtttttga
     5101 ttatcttttt cactgtaaaa taatctactc gttaaacttt taggataatc tttgatagaa
     5161 tatgcatccc aaacaattct ttgaacatat tcacgatcta tattttctct gacttcccca
     5221 gtccaaaaat gttgaccata atggcatctt tctattacat tgttagcaga aacttcaaaa
     5281 taggattgtt ccctttgtat cctcatacca atcatcatag cgcaggaaat aactattaat
     5341 aacaatcctg tttttaatct attattccac atgcagtttc cacatcctta tcttgataaa
     5401 aatcctcttg accctctcca attactgttt taaatataac caccaaaaca agatcatgct
     5461 aagaaatcca agagtatata agatgataca ttctttgtta attatccaaa aaactctgat
     5521 ttctttgagc ttttcacgca ttaggaaaca ccttgttata tcttttttgt atttctttca
     5581 attgtt
//
LOCUS       DAHEYX010000169         5479 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHEYX010000169 DAHEYX010000000
VERSION     DAHEYX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.170"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            38..379
                     /locus_tag="MB219_002204"
     CDS             38..379
                     /locus_tag="MB219_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8427906.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            688..1731
                     /gene="pheS"
                     /locus_tag="MB219_002205"
     CDS             688..1731
                     /gene="pheS"
                     /locus_tag="MB219_002205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCC8427907.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            1736..4156
                     /locus_tag="MB219_002206"
     CDS             1736..4156
                     /locus_tag="MB219_002206"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCC8427908.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(4326..5336)
                     /gene="trpS"
                     /locus_tag="MB219_002207"
     CDS             complement(4326..5336)
                     /gene="trpS"
                     /locus_tag="MB219_002207"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCC8427909.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         1820 a          965 c         1187 g         1507 t
ORIGIN      
        1 taaggattat gctatactct gggaagaggt gagtatgatg gagctaacac agacagaatt
       61 cgttttatgc cctaaatttg aaaaaacatt ctctatttta ggaaaaaaat ggaacggatt
      121 gattatcgat gtactactcg aaaacggtcc gcaacgcttt gttgagcttg cggcaaaaat
      181 cccgatggtc agtgatcgag tattagtcga acgcttgaag gaattagaaa aagaaggaat
      241 agtcactcga aactttgcct gtagcgaaaa gagccgttca gaatatctct tgactgaaaa
      301 aggagcagac ttgcaaaaag cgatgcgtca aatacaagtt tggagcgaaa aatggatcac
      361 agaggaagaa tgcggttgat tttgtaaaaa aaatcttgta aactatagtc catacgaata
      421 agtaaagaac gatgacgaaa aagagtagtt tggctaaacc agttcaggga gatctgtcgc
      481 agactgaaag cagatcctgt gtttgaaaaa tgaagttcac tttccgagtt cgtctgataa
      541 aatcagaccg gcaattgtcg ttaacgaagt aagagtgcgg tgtaagtcat ttatccgcaa
      601 attaggatgg taacgcgaag acctcgttcc ttaacaggaa tgaggtcttt ttgttttgta
      661 tttggaattt aaaggaggaa caagcacatg tctctacaag cacaattaga gagcttgaaa
      721 gaagcaacgc tcgtcaaaat cacagcggca caagatttaa atgaattaaa tcaaatacgt
      781 gttgaaacgt taggaaaaaa aggaccgatc acagaagtat tacgtgggat gaaagatcta
      841 agtccagaag agcgaccaaa agtcggaagt ttcgcaaatg agatccgaga tcttttgaca
      901 gaagagatcg agcgaaaaaa acaagtatta gaagaagaag cgttaaatgc agctttggca
      961 aaagaaacaa tagacgtgac actacctggt agaaaagtaa aacatggtac acgtcatatc
     1021 gtgacacaaa tcatggaaga aattgaagat atttttgtgg gtatgggcta tcagatcgta
     1081 gaaggatatg aagtagaatc tgatcattac aatttcgaac ggatgaatct gccaaaagat
     1141 catccagcaa gggacatgca agatactttc tatatttcag acgagatatt gattcggaca
     1201 catacttcac cagtccaagc acggacaatg gaaaaacatg atttctctaa gggtgctcta
     1261 cgaatgatct cgccagggaa agtcttccgc agagatacag atgatgcgac ccacagtcat
     1321 cagttccatc aaattgaagg gctagttgtt gataaaaaca tcacaatggg cgaccttaaa
     1381 ggaacattag aagtcgtaat gaaaaaaatg tttggggaag atcgtgaaat tcgtttgcgt
     1441 ccaagttatt tcccatttac ggaaccatca gtagaagtag atgtcagctg tttcaaatgt
     1501 ggtggtgccg gttgtaacgt atgtaaatac actggatgga tcgaaatttt aggagctggc
     1561 atggtacacc cgaatgtgtt gaagatgtca ggaatcgatc cagaagaata ttcaggcttt
     1621 gctttcggcc ttggaccaga ccgcgtagct atgttgcggt atggagtgaa tgatatccgt
     1681 aatttctatc aaaatgactt acgtttctta agtcagttca agggggagta agaaaatgtt
     1741 agtttcatat aaatggctca atcgttatgt agatttatcg aatgtaacac caaaagaatt
     1801 agcggataag atgtctgtga caggaatcga agtagaggga gtaacggttc cagaagaagg
     1861 cttgaaaaaa atcgttgtcg gtgaagtcaa ggaatgcgta ccacatccag attctgatca
     1921 cctttctatt tgtcaagtcg atattggcga agacgaactg tctcaaatcg tttgtggagc
     1981 gcctaatgtg aaggttggaa tcaaagtgat tgttgcactt cctaactcac gtatcgctgg
     2041 taatgtaaaa atcaaaaaag gaaaaatgcg tggccaagta tcaaatggca tgatttgctc
     2101 attacaagaa attggctatt cagatagtgt tataccgaaa gaatacgctg aaggtatcta
     2161 ttatatgcca caagaagctg taaatggcga tccagttttt ccttatttgg atatggatga
     2221 ttcgattatt gaattatcta tcacaccgaa tcgtgcggat gcattgtcaa tgcgtggcgt
     2281 agcttatgaa gttggggcaa tttatcgtca gactcctaca ttcgatgacg aaaagctggt
     2341 agaatcctca cgtcctgcta gcgataaaat tcaagtggaa gttgaagata aagaagcagt
     2401 tcctgcttat caaatcagaa tcattgaagg agtaagaatc cagccaagtc ctcaatggct
     2461 gcaaaatctt ttgatgaacg aaggcattcg tccaatcaac aatgtagtag atgtgacgaa
     2521 ctatattttg cttttattcg gacaaccact acatgcattt gactacgaca aattgaaatc
     2581 aaaagagatc tttgtccgcc gtgcaaaaga aaatgaaaca ttagtaacac tagatggaga
     2641 atctcgggaa ttgacaactg acaatctcgt cattactaac ggcaaagaac cagttgcttt
     2701 agctggtgtc atgggagggt tagactcaga gatcacgaat gaaacaacaa cagttgcact
     2761 agaatctgct ttatttgatc caatttcgat tcgtcgtaca gcaaaacaat ttaatttgcg
     2821 tagtgaatcc tctgcacgtt ttgaaaaagg aatcaaccat gcgactgttg gagaagccag
     2881 tgaggttgct gcagcaatga tcgtttctct tgcgggtggg gaagttctac aaggcgcggt
     2941 caaaggctca gagatcaaag cagaagacgt atcagtgaag atttcacttg aacgcatcaa
     3001 tcaatattta ggaacatctc ttgtcgttaa agaagtaaat gagatttttg aagcacttgg
     3061 atttagctat gaagaaaagg agtatgttta ttccgtagta gtaccaccaa gacgctggga
     3121 tatccaaatc gaagcagata ttatcgaaga agttgctcgt atttatggat acgaccagtt
     3181 gccatctaca cttccaagtg gagaaacggt tgctggaagc ttgtcaaaag aacaagaaac
     3241 tactcgtaaa attcgtacaa tctttgaagg aagcggattg agcgaagcaa tcagttatgc
     3301 attgacaaca gaagaaaaat caagacagtt tgttataaat gaaagtaata ttacacgttt
     3361 ggactggcca atgagcgaag aacgttctgt attacgaatg aatttgatca gcggactatt
     3421 agatgatatc agttataaca ctgcccgaaa aaatacagag attgctttat atgaaattgg
     3481 tcgggtgttc ttccaagaaa atgatccacg tacacatctg ccgcaagaag taaaacatgc
     3541 agcaatcgct atttctggta tttgggaaga aaaagattgg cagacgaaag ctcaaaatgt
     3601 agactttttc accatcaagg gtcttttaga aaacttattt gaacaactag gcatcactga
     3661 agatgtccat tatcaaacag tgacagaaat gaaggaaatg catcctggaa gaactgctgc
     3721 gatttacctt ggcgaaaaat ttattggttt tgtcggtcaa gtgcatccaa cgattacaaa
     3781 aagttatgat attccagaaa catatgttgc tgagtttgat ttatctgcta tagtggaagc
     3841 tgctcaaaaa gggatcgttt tcgaagcagt gacaaaattc ccagcagtca gccgggacat
     3901 cgctttgctt gtaaaagaaa ctgtgacaaa tcaggaatta acagaagtta tccgttctgc
     3961 tgccggaaga tttttaacag atatccaatt atttgatgtc tatcaaggag aaaacattga
     4021 aaaaggacat aaatccatgg cgtatagcct gacattcgtc aacccagaag caacattgac
     4081 agatgaagaa atcaataaag caatggaaaa agtcgaaaaa gcgttggtag aaaacctaga
     4141 ggcaagtatt cgctaagaag gaaaatatag taagtaacga tcaagtagct tttgaagacc
     4201 atacatttga aaaaaagatg tgacacaact tcgtcacatc tctttttttg attttaaaaa
     4261 aggaatagca cagagacgag ataaaaaaac aggcctttac agcctgcttt tttagagtaa
     4321 atgaattaat ggaagtaatt gattcccatt gctgatttta cttcagaaag tgtttgtgca
     4381 gctactttag cagcttcttc actaccttta cgtaagattt ccataacagc tgaaggatct
     4441 ttttccaatt cttctcggcg cgcacggatc ggtgcaaatt cagcttccaa tacatcaatg
     4501 aggtatcgtt tgatctttac atcaccaagt ccgccgcggc ggtaatgagc tttcatttct
     4561 tcaattgctt ctttatctgt tccgaataca tccaagtagg tgaagaccat attcccttct
     4621 acttgtccag gatcttgtac atggatgtgg tttggatcag tatacatact catgactttt
     4681 ttctgtaaga catcggcagg atcggagata taaataccgt ttcctaacga tttactcatt
     4741 ttgccatttc catcgattcc aggtaatcta cccattcctt ttggtgggaa tacgcctttt
     4801 ggttccacca agacttcacc atatgtatga ttgaagcttt gtacgatttc ctgtgtctgc
     4861 tctagcattg gtttttgatc ctcaccaaca ggtacaagat tcgctttgaa tgcagtgata
     4921 tctgctgctt gagaaacagg atagataaag aatcctgtag gcacgctttc accgaatttt
     4981 ttttgttcga tttctgtttt gacagtcgga ttgcgccgaa cgcgtcctac gctgactaag
     5041 ttcaaataat acatggttaa ttcagctaat tccggaattt gtgattggat aaataaagta
     5101 gattttgcag gatctaatcc cacggctaaa tagtctaaag caacttgcaa gacatttgat
     5161 gaaaccttct caggattttt agcattatct gtcagtgctt gcatgtcagc aatcatgaca
     5221 aacagttggt tgtcttcatc tgcctgcata gccactcggg tttttagaga accgacgtaa
     5281 tgccctaaat gcagcttccc agttggtcga tctcccgtta aaataatatt tttcaaaaaa
     5341 gacacctctt tacgatttgt aagtctgagc acttaatatc ttaccgtttt tcctgaaaag
     5401 aaacaacagt tcctaaggaa aaaacaagga aagaggataa aataactaat agattagaaa
     5461 tggaagaaat atttatggg
//
LOCUS       DAHEYX010000170         5466 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHEYX010000170 DAHEYX010000000
VERSION     DAHEYX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.171"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..271)
                     /locus_tag="MB219_002208"
     CDS             complement(<1..271)
                     /locus_tag="MB219_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427910.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLIT"
     gene            complement(313..534)
                     /locus_tag="MB219_002209"
     CDS             complement(313..534)
                     /locus_tag="MB219_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427911.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(531..821)
                     /locus_tag="MB219_002210"
     CDS             complement(531..821)
                     /locus_tag="MB219_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427912.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(818..2002)
                     /locus_tag="MB219_002211"
     CDS             complement(818..2002)
                     /locus_tag="MB219_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCC8427913.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(2184..3587)
                     /locus_tag="MB219_002212"
     CDS             complement(2184..3587)
                     /locus_tag="MB219_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCC8427914.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3609..4382)
                     /locus_tag="MB219_002213"
     CDS             complement(3609..4382)
                     /locus_tag="MB219_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427915.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4392..4769)
                     /locus_tag="MB219_002214"
     CDS             complement(4392..4769)
                     /locus_tag="MB219_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCC8427916.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4790..5104)
                     /locus_tag="MB219_002215"
     CDS             complement(4790..5104)
                     /locus_tag="MB219_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCC8427917.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         1613 a         1083 c          924 g         1846 t
ORIGIN      
        1 gggtaatcaa atctttatga ctttcaaaaa agtctaaatc atcaaaagga actcttattt
       61 taagatatgt gcaaagcgta ttccagccct ttttatatcc tttataataa gtaaaatcgt
      121 cagtctcatc taaagttacc tctgatattt tcattttaga aacaatctcg ttacctcctt
      181 cataataatt tgaagagctc tttctttcta atgttttaat taaaagagat ttaaattgat
      241 catttgtaaa catattcata aatcctttca taacaaaata tatacttcct attttatcat
      301 gaaaaatatc tgttaagcac caatgattcg gttaaataag tttaataaca catctttcac
      361 gccagctgca ttgaacacaa gtccgacggc gattagagca ataatcaaga agccaatgag
      421 ttttgaaaat tctcgtttaa aacctaaaaa cagtccaatg acaacaatcg ctaacaatac
      481 caatgactgt gcattatcca agaaccagtt gtacaaattc tgcccaaaat tcatgtgatt
      541 gtcctccttt ccttaagata tttttctcga taatagatat agctttttac acgccttaat
      601 aattgaaaag gggttaaatg ctctccatag acggttactc gattggtata attattataa
      661 aatctcacct tgtaaaaatc ataggtatag atggtatcac cagcttgccg ttttccctca
      721 ataatgagag acgtatattt ccctgtattt tgtatatacg cttgaaactc aatgccacac
      781 tgaaacccta acttctttaa ttcttttgtt aaaaggctca tgtaatcaaa tcctccatgg
      841 ctagatgttg ttgttcaatt aatttctcat gtttttctgt cagtttggct tcctgaatca
      901 tggtggagat aatggttgta ccgttgagaa catctaaaat gcttgcgatt tttaatgtag
      961 gggcgacttg atgatgtaac cagttcaatg ttcgttcaaa agaataaggc tctggctgtg
     1021 tggttaggcg taatgctcca cgatttttac cgataaacca ttcccaacgt tcattagttt
     1081 tccaatcact ccgacgttta gtgtcgtctt tatctgcaaa gcggatatag cgattgataa
     1141 tttcaaaggc tgttttttcc gcctgttgat gtgccaataa atctttcatg gcttgggtgg
     1201 ctcggtcatt ttttaaccga atttcaaacc gattctttac ttctgcgtct tctaagggaa
     1261 catcattttt cacgtattgc tcataatctt tctcatatat acagaaatag acttcacttt
     1321 ttaatgagcc aatataaagt gtgtttccca tgtatggttt ttcatcacgg tgaaccaatt
     1381 caccactgcg ataattttta aagcttcgaa agacggaaat gcattcttcc tgtttacatt
     1441 tctttgccaa atctggaata ttcaaaatcc ctgttttatc gttgatagct aaatccaagc
     1501 gtttgaaaat acctttatgt tccatgcaat cttgaaagaa atcataccag ctccgatgtt
     1561 gagccaatag aaagttttca aattgccgac agccttttcc tttcagttct aataaaacac
     1621 ctttacttac atcatgagag actaggacaa caatatcacc gaaacgatag tgttcgggat
     1681 aagaataata gccaaagtct tcgtgtagca tataatccat attcaaacgt aaaacctcac
     1741 cgataatttg agccacatca ttggtaggga atcgaatccg tacatagtcg aataacatct
     1801 ccaatgggtt atcgggattg aagcgttcca aagcgttcag taaatcttct ttcacctgtt
     1861 gagtagggac tgccttgcct gtttcaatat ccgataggta aggtctggta atatgagaag
     1921 caaccgccag tttattttga gaaacgccat aagccagtcg cttttctttc aattccttaa
     1981 tccaaatttt ttcgttttcc attcgcaaac ctccaatcta aaaaagatgt aaactatgac
     2041 ccttgagttt acacctttca tattttccgc aatcctttgt accatcaaca ttggttgatg
     2101 agaattgcct gttttcagtt gtatttgtgc ccccctgtta gatagagggg gctaaaggat
     2161 tggcgtggct aacgccacac caatcaaagc aagtctgaac ctgtaccttt cacttcgtgc
     2221 ggtacaggct cagattgctt tttttgaatg acttttccta tttctcctaa gaaatcgtac
     2281 ctttttggta caaggggtgt ataaaactca ctaatcacac tttttcctgt atccacgtaa
     2341 cctcttccct tgatgggttt taagaaaaat tgtttttgtg tatcgcttcc gaacatcata
     2401 ccaaagccca tttcagacat acgacccaaa gccacacgga agttaaagtt gtcccgaatc
     2461 ccatcactaa aatattttgc gtctggacgt tgacaggcaa gaattaaaaa gaagcctgct
     2521 tgtcgtccta acattacaat ttgtttgagt ttatgaataa cggtggtact ttcacgacct
     2581 agcatatcca tcattgccac atattcatca aaaataagaa aattggcaga caaaccaaga
     2641 taagcataat tttctcctgt tttatagtta gggtgttgtt tcatggtttc actgcgatca
     2701 atcatatctt cgtaaaattg attgatacat gccaacatat cttctttacg gtaatagaca
     2761 ttattcatga ccgcccctaa gtctgccaaa tcagcatttt tagggtcaag aatataaagc
     2821 tttgcatttg tatgcaggag agattcaatc aatgtgagaa taaaatagga ttttccacta
     2881 ccagtgcccc cagcaattaa catgtgaggg agagaatcga actcccaata gaccgattcc
     2941 atcaatttga cacgtccatc ttgagctacc acgtcttcaa tgccaattcg attgccaata
     3001 gtatcataaa gtaggacata ctctacataa gaatctttca acgttttttc cactaattca
     3061 caatacaatc ctgtttctag tttgttttct aggtgcaaaa gctgatcttg atatttccct
     3121 aaggtgattt ctacatgaat atggattagt ccatctttca ttcgataata tattttagga
     3181 aaatggcgga ttcgttgttt ggttttcttg ctgggtaaat ctttaaagaa tccctctgat
     3241 tgagtttctt cggcttcata ccaatgattt tctaaaatca tacgagctaa cttttgacgg
     3301 tgctctaact gttttataga atctatacga tagtgataat aaagccagct gataagtccg
     3361 catacaaaaa gagcagtgag aatactgctc caaaagtatg gcgtatgaag ttgatagaaa
     3421 aatgtatcta actgaactgc ttgccagttc accgattgta acgctctcca atgaaaaggg
     3481 agtaggacga ctagaaaaac taccagcaat gaaattagta caaaatgata gaccaagtgc
     3541 ttatcgttgg ctttgattcg ctctcctctg tcatgaagca atggcataaa aatcctcctt
     3601 tccacctatc aaaataattg tgtattgtag atacgagcct gtttatcaaa agttggttga
     3661 gaaatggctt catatttctg ataagcgatt cgcttttcaa agcgtaaatt tttttctttc
     3721 ggataataat gacgtcgata gagagtgtgt cctaacagtt ttttcgcttc ttttgtcaac
     3781 ggatagagat agcgaaacat gcgtccattg attttctcaa tccctttgta ttcgcaaaat
     3841 gcctgggaaa gccaatgctt tttctctacc tgttcaaagc gagcattttc ctctaataaa
     3901 aggcgagcac ttcgagggtg tatcttttcc catgactgct tatcacgata gacacttgtc
     3961 ttgaaatacc cgcagtaaaa gaagttggac gcttgataga catagccaca ttttccctca
     4021 attccatctg ccagtgtata taaaaaatga cagtccgtat gttctttcaa ccacttaatt
     4081 agagctgaaa gagcttgact gccaaaataa ttggtttgat tcatttctgg aagaaaacac
     4141 atctttccaa tttctaaata atctgtggtt tgaagggaac tgtcgggaaa gagtttgcgt
     4201 atggtttgta ggggttgcgt accccagcct aattccacaa cgcctaataa tttttcttct
     4261 gaaaaaatcc ctagaaagta tttacacaat ctagggagta tctttgaata atggtattgc
     4321 cgaataaaag aaatggcttc atccttcgga atttctttca aaatcaatgg tgtgtttgtc
     4381 ataagcaaca cctatttttc actttcggac gcttcttttt tatatttctg atgaggtttt
     4441 gtctgtcctt ttaatacaag atcgtctgct ttcacgtacc aatcaacgtc tgcccctcga
     4501 aaagtagccg tcgctacggt atctgccaca ggattgatta attccacttc tgcgttgtag
     4561 tcaaattctt tcaaaggaac agaagcgggg atactgactt gtatcatgcg tccttgtcct
     4621 tttgatttta agtcataggt acgttcttta atttcttcgg aaaccgtacc atcttcattc
     4681 tggatacgaa cctcacgacg caaggcggaa aatttcaatg tgccaaatgt ctggtctttt
     4741 tcaatcacaa taccttctgc taatctcata ttttttcacc tttcctttct tatgctttca
     4801 ccatatcgtc tgcgtgcata atgtaattgg taaatcctcg attgccaatt ttataacctt
     4861 ctgcggtaat atgtgggttg atgagtttga ctttttcgtc ggattcaaaa tgtttctcgc
     4921 cagcttcgga gggaagaatg acaacaatat catctgctcg ttgaatcgtt gagtataaat
     4981 tgtaactacg agagagaatg gttggtctgc catttattcg acgttgttca atgttccctt
     5041 caccagcata ttctaattgt ccaaatgttt tttccatatt gggaatgaca tatttaagtt
     5101 ccatattttt acctatcctt tctgttttta tgtttgaatg aatcttattt gtgtgatggt
     5161 tttaaagggg ggagagcgac cttttgattc ttgatttttt attttcataa gttcacttcc
     5221 tttcaaaatt gggtaaaaaa atagacgctc cgtactgagc gtccgattcc tactatattt
     5281 aaaacaacga ctacctaggt attttaaata ttataatcaa attctatttt atctaataat
     5341 tgagtgggat aaagcaagtc tcctttactc gtaaaataac ccacctctga gaaaagaaat
     5401 aaattatttt ttgctcttga acaaattacg tatagtagtt ttctcgctga actaattcct
     5461 ttatca
//
LOCUS       DAHEYX010000171         5455 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHEYX010000171 DAHEYX010000000
VERSION     DAHEYX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.172"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(68..688)
                     /locus_tag="MB219_002216"
     CDS             complement(68..688)
                     /locus_tag="MB219_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCC8427918.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(764..1201)
                     /locus_tag="MB219_002217"
     CDS             complement(764..1201)
                     /locus_tag="MB219_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCC8427919.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1364..2194
                     /locus_tag="MB219_002218"
     CDS             1364..2194
                     /locus_tag="MB219_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCC8427920.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2178..3545
                     /locus_tag="MB219_002219"
     CDS             2178..3545
                     /locus_tag="MB219_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCC8427921.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3564..4373
                     /gene="yidA"
                     /locus_tag="MB219_002220"
     CDS             3564..4373
                     /gene="yidA"
                     /locus_tag="MB219_002220"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCC8427922.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4416..4859)
                     /locus_tag="MB219_002221"
     CDS             complement(4416..4859)
                     /locus_tag="MB219_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCC8427923.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4993..5364
                     /locus_tag="MB219_002222"
     CDS             4993..5364
                     /locus_tag="MB219_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCC8427924.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
BASE COUNT         1663 a         1136 c          986 g         1670 t
ORIGIN      
        1 cgtgtcacac ctccttattc tgataggctg atcgtctctg tcacaacctc tcgtaagaaa
       61 ataatgttta ttctttttca gagtcagaaa attcttcctc ttcataatct tcatcatcat
      121 cgtcttcgtc tatgatactt aaatcttctt cgatatttcc tggcaagtct tcttcatcat
      181 ctgtcgggtc atctgctccg atttcttgca agtcagaatt ataagcagcg atttcttcat
      241 cttcatcatc atcgtcatca aacagatcat cgtcatcttc ttctgtcaga tctgtatctt
      301 ctgggtcatc gtcattgtaa tcgatcgcgt cttcatcatc attgataaag gcgttgactt
      361 tcttgcgttt acggcgacgt ggagtatctt catcctcttc gtccattcca tggtttactt
      421 cttcatcgat cgaatcgata gcataccatg aacgcaagcc ccaacgatta tcccctaatg
      481 agataaagct gccgtcaata ttcaagtcag tataaaattg tgcgagtgaa tcacgaattt
      541 cactgtctga tttcccaaga tagttttgga tctggttagc taaatcagaa aaatccatga
      601 catcgccacg ttgttctaag atcgcgtgtg ctacttcgat cattgataat tcgcttttgt
      661 ttgcaccttc aaatacacta atttccaatc aggacacgtc ctttcacagt ctactcctta
      721 tcatacaaaa tcagacacaa aaaatcaaac ttttctttta tatttacgct gtaaaatcca
      781 aggtcaaata atattcgccg attttttccg gtcccatgaa cagatcataa tcgattcgaa
      841 ccttccctgc tgtgggacgg tctttcaaac taacatgcag ttctttcgta aaagtagcaa
      901 tctggatcat accgtatggt gttcgatagg atgtttctaa ccgttcttta tagccaaatt
      961 tcaaacgcat ccgcatttct cctgcacgga tcagctgtac atgaccatct ggaactaatt
     1021 tgatcgttac aggaatctca tcgcccgtct cctcctgaat ctctttataa cgtatgtaca
     1081 gcgtatcgcc aattgtcacc atttgccctt tcaaatcaaa ataaaaatct tgcacttccc
     1141 cttgctgttt tacttttgta cgcaatttga ttgaaatcgg tgttccttgt gataattcca
     1201 ttattcattc tcctttactt ctcatagcta cattgttcct agtttctttt gttttgtcaa
     1261 ccaacttgtc catttttaca gaaaaccttt tttccaaaac aactctttga ttttagagta
     1321 taatattgta gaaaatatga atttatacaa aggagccggt tgaatgaacc ctgcgattcg
     1381 actagatcag cacatttatc aaaaaaacga cttttcccct tttgcattaa cagatatatt
     1441 aacggaatat tctaaagaac accagcaaat ctttttacat ttctggcaat atgactgcac
     1501 agtcatctta ggtatgaagg atgttcgaac tccttttcta agtgacggga tccaaacact
     1561 ggaagatgca ggttacgctc cagtgatcag aaattcaggt ggattaggga tcgtatcaga
     1621 tgaaggaatt ttgaatattt ctttgatttt cccccaaagt gacacaaaaa aaataaccat
     1681 agatgacgct tacgagcaaa tgctcctatt gactaaatac gcttttccaa aggcaccgat
     1741 acaagcattc gaaatcaccc attcttattg tcctggaact tatgatttaa gcatccatgg
     1801 caaaaaattt gccggtatcg ctcagcgtcg gatcaaaaat gggatttctg ttatgatgta
     1861 cttaagtgtc aacggtaatc aggaaaaacg tgggcaagtc atgaagcgat tttatcaatc
     1921 tgctttaaaa gaagactttg gtacaaatgg ctatcctgca gtggatcctg ctacaatggc
     1981 aacacttgaa gaactgcttg accaacccct ttttgtaaaa gaagtcaaag aagcatttgc
     2041 aaaagctttt gaaaagctgt attctgcaag tgatttacag gattctaccg actggctaaa
     2101 aaaagaaatc cagctggagc aatggaacaa ccaaatccag agaatggaac aacgtaataa
     2161 cataaaggag ctacattatg acaacccctt ataaatatca aatgctgcct atggaaaaag
     2221 tttttcgtga tccagtccat aactatatcc atgtccagca tcaagtaatc ttagatttga
     2281 tcaattcgaa agaagtccaa cgtctacgac gcattaaaca attaggcact tcttctttca
     2341 cttttcacgg agcagaacat agccgtttta ctcattcatt aggcgtttat gagatttcaa
     2401 gacgaatctg cgatatcttt tctcgaaatt tttcaaaaga aaaaataggc aacggaggct
     2461 gggatgatca ggaacggctg gtgactttat gtgcggcact gttgcatgat gtcggtcatg
     2521 gtccttactc tcatacattt gaacatatct tccatacgaa tcatgaagcc atcactgtag
     2581 agatcatcac ttctccagaa acagaagtgt atcagattct taaccgagta gaagaaggtt
     2641 ttcctgaaaa ggtagctagt gtgatccaaa aaacttaccc caacccacaa gttgtccaaa
     2701 tgatttctag tcagatcgat gcagatcgaa tggactattt gcttcgggat gcttatttca
     2761 ctggaacaga atatggaaca tttgatctta cccgaatatt acgtgtgatt cgtccttatg
     2821 aaggcgggat cgctttttcc atgagtggca tgcatgcagt ggaagattat atcgttagcc
     2881 gatatcaaat gtatgtgcaa gtgtacttcc atcctgtttc ccgtggtatg gaagtaattt
     2941 tggagcatct gcttcatcgc gcacacgagt tgtatcaaga agatgccagc agttttgaac
     3001 tccattctca attacttctg ccatttcttg acggaagctt tactttagaa gaatacctca
     3061 aacttgatga tggggtcttg ggcacttact ttactcaatg gtctgaatct gctgatccta
     3121 ttttaaatga tttagctaaa cgttttttga atcgtaaacc actgaaatct gctacttttt
     3181 ctggcaaccg ggattcaaaa ttagtccaag aactcacttt gttagtagaa aaggtcggat
     3241 ataatcctgt ttattacact gctgtgaatt ctagctacga tctaccttat gatttttatc
     3301 gcccagaaca aggcagacat cgcacacaaa tcgagatttt gcgaaatgac ggaactttga
     3361 tagaattatc acaggttagt cagcttgttg ctgcattagc gggacaagaa caaggagatg
     3421 agcgattttt ctttccaaaa gaaatggttg atccgtcctt gcgtgatcat tatgatttat
     3481 ttgacgaaac ttatcaagaa tttgccagcc atatccgtaa tggcgcattg attgaaataa
     3541 attaaaatat attggaggaa attatgtcta tcaaactagt cgctatcgac atcgatggta
     3601 ctttattaaa tgagaaaaga gaagtaacaa aagaagtaaa agaagcgatt gccgctgctg
     3661 ttgcaaaagg tgtttcgatc gttttatgca ctggccgtcc cttaccaggt gtccaggaac
     3721 agttaaacga gttgaacctt tttcaagata atgattacgt tattacttat aacggggcac
     3781 tcgtacaaca aacgaaatca ggtaaaatca tcgcccgtca cggactgaca catgaagatt
     3841 tcctagagat cgaagtaatg gcacgccgtg tcggcagtca tctccattcg atcgatgaca
     3901 aagcgatcta cacgcctaat cgagatatca gcgcttatac gatccacgaa gcaagtttag
     3961 tcaagatgcc gctaaaatat cgaacacctg aagaaatgac accagacatg aacatcgtca
     4021 aaatgatgat gatcgacgaa ccagaaattt tagatgcagc aattgcgcgt ttaccgcaaa
     4081 ctttccgtga taaatacaca acggtcaaaa gtgcgcccta ttacttcgaa gtactaaata
     4141 aagaagcaag taaaggtgcg gcagttgcga atctcgcaca gcaccttggt attgatcaag
     4201 atgaaatcat ggctatcgga gataacgaga acgacttatc catgattgaa tatgctggtc
     4261 taggagtagc aatgggaaat gccgtttctc ttgtcaaaga agcagcaaat gtcatcactt
     4321 catcaaacga cgagcacggt gtagctgaag caatcaaaaa atatgtactg taataaataa
     4381 aaaaactgag agaaagctga tctactcgct ttctttcagt ttttattttt ttcgatccat
     4441 ctctcgactg cccatcggtg gctaccacgt ttcaatttca caagcgcttc ttgagggctc
     4501 acccaccaaa ttttattggt cttttcagtt ggttctccta tacgcttcca agaatcagca
     4561 acaaagaaat atcctggatt ataataataa gttgcacgat aattcgagta aaaatactca
     4621 tcagcttctc ccagataaga agaaatttct gccacaattc ccatttcttc tatcagctca
     4681 cggacaatcg cttcttcttt cgtctcttct ccttcaattt ccccgccagg aagaaaaaaa
     4741 gcaccatttg gagcctcgat tactgcaatc tgctgatctc tttctatgac aatatatgct
     4801 gcataacggg tttgataatc agcaaattgc tttttttcac caaaaattgg tttatccatg
     4861 aatagtttcc accttaacag ttttattttc tatccatttt acatgagaaa tttctcatga
     4921 acaagcacaa atcaactatt tccttacttc tttttgttat gattgagtaa aaggaaaagg
     4981 agagatgaat ctatgaaaat ggctcacaca tgtgttcgag tgaaagatct tgaagcatca
     5041 atcgaatttt atcaaaaggc atttggtttt gaagaaagcc gccgccgtga ttttccagaa
     5101 aacaaattta cacttgttta tttgacactt cctggggatg actatgaatt agagttgact
     5161 tacaactatg atcatggtgc gtatgatcta ggagacggct atggtcatat cgctatagca
     5221 gctgatgatc tagaaaaatt acacgagcaa cataaagctg ctggtcttga gattactgac
     5281 ttaaaaggat tacctggcac tgcgccttct tactactttg tcattgaccc tgatggttat
     5341 aaaatcgaag tcatccgagg atagttagta tggggcattt tactcttgat tgaataaaaa
     5401 acaaacacca ataattaaaa tgagacgcaa acttttcatt aaattgaaaa agttt
//
LOCUS       DAHEYX010000172         5408 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHEYX010000172 DAHEYX010000000
VERSION     DAHEYX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.173"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            193..1302
                     /gene="pdhA"
                     /locus_tag="MB219_002223"
     CDS             193..1302
                     /gene="pdhA"
                     /locus_tag="MB219_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCC8427925.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1305..2282
                     /locus_tag="MB219_002224"
     CDS             1305..2282
                     /locus_tag="MB219_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCC8427926.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2307..3950
                     /locus_tag="MB219_002225"
     CDS             2307..3950
                     /locus_tag="MB219_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCC8427927.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            3958..5364
                     /gene="lpdA"
                     /locus_tag="MB219_002226"
     CDS             3958..5364
                     /gene="lpdA"
                     /locus_tag="MB219_002226"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCC8427928.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         1794 a         1024 c         1241 g         1349 t
ORIGIN      
        1 tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa aatttgtgct
       61 tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt taaatagtga
      121 tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag atgagagagg
      181 aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact gctagatgaa
      241 gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt agttaacaaa
      301 gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca tatggtatgg
      361 tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt
      421 gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt tgataaagaa
      481 gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg tttaccttta
      541 tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc ggaagaattg
      601 aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc cggtgttgca
      661 ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg tgatggcggt
      721 tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc
      781 gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa acaaactgca
      841 gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca agtcgatgga
      901 atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc gatcagtgga
      961 aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac actatctggt
     1021 gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa aaaagatcca
     1081 ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa
     1141 caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc cgacaaagta
     1201 ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc acaatcaatc
     1261 aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa
     1321 tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag aatgtcgttg
     1381 tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa ggattgcagg
     1441 aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga atcgcaggat
     1501 tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa ttctttggtt
     1561 ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga
     1621 gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt gttcatacac
     1681 cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt attcgagtag
     1741 tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt agaagtaacg
     1801 atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa gaagtgccgg
     1861 atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga acagatgtat
     1921 ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa
     1981 aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta gatgtagaaa
     2041 cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac
     2101 aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc ctttcattag
     2161 aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga caagcagaaa
     2221 atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc gcggaatttt
     2281 aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaattgccg gatatcggtg
     2341 aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg
     2401 aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg
     2461 ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat gttggggatg
     2521 tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt ggtgtagccg
     2581 cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca gaatcagctg
     2641 gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa ggaatcgcag
     2701 aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat
     2761 tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc acagggacag
     2821 ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta ttagtagaaa
     2881 tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct gaagcaccaa
     2941 aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat ccaaataaac
     3001 gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta gatatctcac
     3061 aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa aacttcttgg
     3121 caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag
     3181 aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc aagtccaact
     3241 taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc gctaaagcaa
     3301 tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta gaagtgtcta
     3361 aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt acgaaactta
     3421 ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac ccagtattga
     3481 atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac aatatcggta
     3541 ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca gaccgcaaag
     3601 gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat gacggcaaac
     3661 tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga tctgtaggtg
     3721 gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt gtaggaacga
     3781 ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac
     3841 tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata
     3901 atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga taaacaaatg
     3961 gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg acctggtggc
     4021 tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag
     4081 tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact gatagctgca
     4141 ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag tgaaaacgtc
     4201 aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca tacgttgact
     4261 tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc
     4321 ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac ttattcgttc
     4381 aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt taaattcgga
     4441 ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa gaaatttgtg
     4501 atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca
     4561 gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa agatctagta
     4621 aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg
     4681 aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa
     4741 gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat
     4801 atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac ggtggataaa
     4861 caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc aggggcggca
     4921 ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa
     4981 gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga attggcttct
     5041 gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc atataaattc
     5101 ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt catccgtcta
     5161 gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt
     5221 gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga agatatcgcc
     5281 ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc ggaattagca
     5341 ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca
     5401 gttgcgtt
//
LOCUS       DAHEYX010000173         5374 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHEYX010000173 DAHEYX010000000
VERSION     DAHEYX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.174"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            219..1493
                     /locus_tag="MB219_002227"
     CDS             219..1493
                     /locus_tag="MB219_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8427929.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1547..2974
                     /locus_tag="MB219_002228"
     CDS             1547..2974
                     /locus_tag="MB219_002228"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCC8427930.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2987..3859
                     /locus_tag="MB219_002229"
     CDS             2987..3859
                     /locus_tag="MB219_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCC8427931.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3954..4949
                     /locus_tag="MB219_002230"
     CDS             3954..4949
                     /locus_tag="MB219_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8427932.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
BASE COUNT         1857 a          742 c         1043 g         1732 t
ORIGIN      
        1 attgacattt atttataaaa aatagtgcca ttaaaaaaga aaagtgacac accaataaaa
       61 gtcatatcct gtgacattat atggcattat ccaaatagaa taaccggttg acatgtaagc
      121 ggattccttt tataatttta ggagtataag tccttttcta aaaatattaa ccggttatca
      181 tgaaagggga tacaaatata tcgttggagg ataaaaatat gtcaaaggtt aacgtattta
      241 aaaatcaatc ctatttacaa agttcagcta cattattact attttttgct tcttggggtg
      301 tttggtggtc attttttcaa ctttggctaa catctgaatc aaatggttta gggttatctg
      361 gcagtgctgt aggaacagta ttctcggcaa attcgttagt taccttaatt ttgatgttta
      421 tttatggaac attacaagat aaattgtata ttaaacgaaa tttattaatt tttgcttctg
      481 tattagcgac acttgttgga ccatttttta tatggatata cgggccattg ctagataaca
      541 attttaattt aggcattatt atgggagcac tatttttgtc agctggatat ttagcttctg
      601 taggagtttt tgaagctgtg tcagaaaggt ttagtcgttt atttggcttt gaatatggac
      661 aagcaagggc gtggggatca tttggttatg ccttggtagc gcttttggca ggatttttat
      721 ttgtaaaaaa tcctcattta aacttttggg cgggatcttt ctttggttct ttactattgt
      781 taaatttatt attttggaac cctaaagttg aacgggaagc aaatcaaaat tttaatcaag
      841 aacaagctga atcaaatagt attccttctt taaaagaaat gtttgatcta atgaaactgc
      901 ctcaattatg gacgataatc atctttattg tttttacatg gacattttat acggtattcg
      961 atcaacaaat gtttccggga ttttatactg gtttgttttc aacatcagct aatggtgaaa
     1021 aaatatatgg gacattgaat gctattcaag tattttgtga agcgttaatg atgggaattg
     1081 ttccaatcat tatgagaaaa ttaggggttc gaaatacttt gttattaggt gtaaccatta
     1141 tgtgtgtacg aattggattg tgcgggtttg cctcgacacc attatctgtt tcatgcataa
     1201 aaatgttgca tgctttagaa gtaccattat ttacattacc aatgtttcgc tattttacac
     1261 ttcattttga tacaaagcta tcagcaaccc tctatatgat aggatttcag atagctgctc
     1321 aaattgggca agtgatttta tcaacaccat tgggaatatt aagagacaac gttggctatc
     1381 aaccaacatt taaaattatt tctcttattg tattactagc aggcatatat gcattcttta
     1441 ttcttaaaca agatgataga gatgttcaag gggatccatt tattcgagga taatggaaac
     1501 caacagaata aaaaacaatt ttatctagaa agtgagcgaa tatattatga caaaaagagc
     1561 tatagaatta acaaatacac gttatcgttt gggatatcat gtgtctgcac catcaggatg
     1621 gattaatgat ccaaatggat tttgctattt tgatgggtat tatcatgtgt tttatcaaca
     1681 tcatccatat agtgctgaat ggggaccaat gcactgggca catgctagaa gtaaggattt
     1741 agttcattgg gaatctttac cacttgcttt gactcctgga gatcaagaag atgaaggtgg
     1801 ttgtttttca ggaagcgcta ttgaaaaaaa tggtgtgttg tatctgttct atacaggaca
     1861 tcactattat ggagacaatg atccagatca tttttggcaa aatcaaaaca tggcgtatag
     1921 tacggatggc attcacttta ctaaatatga aaaaaatcct gtaatagcaa aagcaccaga
     1981 agataacaca catcatttca gagatccaaa agtatggaaa cacgatgaaa aatattacat
     2041 ggttttgggt agtcaagaaa aagatggatt aggtcgtgct attgtttatc agtcgttaga
     2101 tttattaaat tgggattaca ttggaccaat ttctaaagcg aaggaagtac ttacagaagg
     2161 atttatgtgg gaatgcccag acttttttga attaaatgga aaatctattc ttttattatc
     2221 ccctcaggga attgaagcaa atggaaaaga ttatttaaat ctatatgaaa caggctactt
     2281 tatcggtgat tatgattatg agacagcaaa attcattaga ggaaattttt atgaattaga
     2341 taagggacac gatttctatg caacacaaac aactttatct ccagatggta gacgtattgt
     2401 tatggcttgg atggatatgt gggaaagccc tatgcctgaa aaagtagacg gatgggcagg
     2461 agcattaacg cttcctcgag aattagagtt aattggagat catttgtata tgaaacctgt
     2521 gagagaatta gaacaacttc gagttggcag cggcgttgaa acaaaagtta atttggcagg
     2581 agaaataata atcgctcaag atgcttcatt caaagaagct ctaattgatg tactattgac
     2641 taaatcaaaa aatcaagaaa tcaaattttc attaaaaaca aaagatgaag agttaattaa
     2701 tattttttat tctaaggata aaaatgaatg taccttaact cgtaacggca aaaatgatcc
     2761 aaggtatgga accattcaga tgtgtgatag attatcactt cgagtgttta tcgataaaag
     2821 ttcggcagag atttttatta atgatggcga gcttgttttt acagagcgtt attatacaga
     2881 aaatcaacca agtatgcatt tgagtgttag tgaagaagct tttgtctcga gtacaattta
     2941 tcatctaaaa aaagatgcaa tttcttacga gtaaggaggt tttatgatgt taattggtag
     3001 catagaagca ggtgggacta aatttgtatg tgccgtggga gacgaggatt accgagtaca
     3061 agaccaaata atttgtccaa cgaccacccc cagggaaaca ttgaaaaaaa ctgttgacta
     3121 ttttaaacag tttgatataa atgcattagg tattgcatca tttggtccaa tagaaattcg
     3181 aaaaaattca ccaaaatcag gatatattac gtctacacca aaggtgggat ggcgaaatgt
     3241 taatgtgtta ggttatttac aagatgagct gaatgttcca atgtcttgga caacggatgt
     3301 gaacggatca gcttatggag agtacatcat gtccacttta tcgaatgaaa aaataaattc
     3361 attagtttat tacaccgttg gtactggcgt tggtggtgga gctgttgttg acggaaaact
     3421 attaggatcg tttggtcatt cagaaatggg ccatacgttt gtaaaacgcc atcctgatga
     3481 tattgatttt aaaggaattt gtccttatca tggtgattgt ttagaaggct tagttgcggg
     3541 accaacgttt gaagcacgtt ttggaaaaaa gggtgaagac gtttctttaa cggatcctat
     3601 ttgggatgta atggcctatt atctagcaca agctgtgatt caaacaacat tgattatacg
     3661 accggataaa atagtatttg gtggaagcgt agtaaatgaa aaattgttag aaaaaattcg
     3721 tgttaatgtt aatgaattat tggatgatta cgtggaaata ccaccgttaa gtaaatatat
     3781 tacgcgtcct ttaattaaaa ataatggatc ggcaacacta ggaaactttg ctttgggctt
     3841 gaatcaatta caagaataaa acatagaaaa aatatcgatt atcaggtata gtagttctat
     3901 agagttcttt ttttcgatag ttaattattg cattatttag gagagacaag agaatgcgcc
     3961 caaaattaga agatgttgcc aaaagagcaa atgtatcaaa aacaactgtt tcacgtgtat
     4021 taaacaatcg tggctattta agtcaaaaaa cgattgataa tgtttataag gctatagaag
     4081 aactcaatta tcaaccgaac gttgtagcgc gtcaattatt tcaaaagaaa actaacattg
     4141 tcgggttact atttcctaca gtagccaatc ctttttttag tgaactcgtt gaggcattgg
     4201 aaaaaaagtt atatgaaatt ggttataagg tattaatagg caattcgatg aataataaag
     4261 aaaaggaaac aaattattta aatcagttat tgtctgatca agtagatggt ttaattgttg
     4321 gtacacataa ccaagggatt caggaatata aatatcagaa tttaccgatt gttgcaattg
     4381 atcgagtgat gaatgaagat attccagttg ttgaatccga taactacaat ggtggaaaac
     4441 ttgcaaccaa gttattaatt gcacaaggtg caaaaaatat tattcataca aacggtccaa
     4501 ttgacttaca gactccggct aatcgtcgga gactagccta tgaagatact atgaaagcat
     4561 atcagttaat tccacgaact gtgacattag atttcaatat ttcttatgta aagaaaaaac
     4621 agattttttt ccaaatgttt gaggattatc ctaaaataga tggtattttt gcttcaaatg
     4681 acatcgatgc ggccctaata ttacaagtag caaaagaaaa ggggttaaat gtaccagctg
     4741 atttattagt agtcggatat gatggtactt taatgactcg gagtatatta ccagatctga
     4801 caacagtaat acaaccaatt aatgatatag cagacacagc tgttgctatt ttgatgaaac
     4861 gaataaataa agaagaaaca aaaaaggaat atattttacc tgtcacatta tggatgggga
     4921 agacatcagt aaagaaaaag gacaattaaa tttataaaga ctatcaatga taatttcatt
     4981 attaattcgt gatataaaaa gttgttaaat ctcttataat agactatgat acaaaatcat
     5041 gcttttaatc tgtgtagatg ttgccaaata tttgaaagac atagaacttg tcagaaaaac
     5101 cttaacaatt cctcaatggg cggataagct aggcaaaagg gctggcataa acttttcagt
     5161 ccttctaaca gaatcaattg cagataaagc agataacatt cttctttctg gaagaaataa
     5221 ttagaactca aaagggtcag aaacttaaaa gtttttgatc ctttattttc aagtctgatt
     5281 tgacagattt tcggcagctc atttatttat ggtcagaaat agttgctgaa attttaatct
     5341 attagcaaat agtccagaac gtatgaagag gatt
//
LOCUS       DAHEYX010000174         5362 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHEYX010000174 DAHEYX010000000
VERSION     DAHEYX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.175"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(76..474)
                     /locus_tag="MB219_002231"
     CDS             complement(76..474)
                     /locus_tag="MB219_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCC8427933.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(471..1244)
                     /locus_tag="MB219_002232"
     CDS             complement(471..1244)
                     /locus_tag="MB219_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCC8427934.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1241..1924)
                     /locus_tag="MB219_002233"
     CDS             complement(1241..1924)
                     /locus_tag="MB219_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCC8427935.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2091..2978)
                     /locus_tag="MB219_002234"
     CDS             complement(2091..2978)
                     /locus_tag="MB219_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8427936.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2975..3844)
                     /locus_tag="MB219_002235"
     CDS             complement(2975..3844)
                     /locus_tag="MB219_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCC8427937.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3981..5267)
                     /locus_tag="MB219_002236"
     CDS             complement(3981..5267)
                     /locus_tag="MB219_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCC8427938.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1640 a         1206 c          893 g         1623 t
ORIGIN      
        1 aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct tcttgtttgt gtttatttcc
       61 tcttcaaacg cttgtctatg attcagactt ttctctatcg ttattttcat ctaaaagttc
      121 ttgcattccg ataatttctc gatattgatg aatcttagtg ataccaatga ctttgtctac
      181 atcaatacta aaacaaatac cattacctgg aatatcgatg ttgatgcctt gaccaatcgc
      241 atccattact ttattcgtca aactatcggg tactaatgtc aacacaatat ctttttctgg
      301 cgaaatgtcc aaacctaaaa atttccctga atcatgtaca ccagaacctc ttccatgtaa
      361 gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg ataacatctt cgcccattcc
      421 tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct acaagatctt tcatttgctt
      481 tcccccaatt tttgcaatct ttctttttcg attctgtctt tttcatcttt tcgtttataa
      541 atatatccta gtactaaagt ggataatatt ggtgtcaatg caatcattgc aaccacacca
      601 aacccgtcaa tgatcggatc tcttccttca atttgtgcag cgattgccac gctcattgat
      661 aaaataaatg tcgaacacat cgggcccgtt gctactccac cgttatcaaa agccattgct
      721 aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta gcccgtatcc tgggatcaag
      781 aaataataaa gagagaatcc gacgagcaaa cgccacatag acaatcctac tgctgcgccg
      841 atcccaattg aaattaccat cagcatgatt ttcgatcgga ttctcccctc gctcaattct
      901 tcaacctgtt tgatcattac atgaatcgcc ggctcagcaa accctactaa gaaccccatt
      961 aaaaagccaa ggggaaccag tatataattg taaggcaatc ccgcaatagc actgcctaaa
     1021 tgctgtccta ccggtacata agccatattg actccgtgca ggaaaaggat caacccaata
     1081 gtcgttatca agaaaccttt cataatgtca aagaaacgct ttttgcgcat acgaaaacta
     1141 atgatattca ttaaaacaaa taggattacg ataggtgcca ctgccaaaat gacttccatg
     1201 gtaacgattt cgattccatc tatcacttgc tgccagaaac tcattggaag atcaccccca
     1261 aaatcatcac ggcaagaatc ggccctaacg aagcaatacc gaccattcca aaactgtcat
     1321 tttcattttt cttttgtttg atcatactag tcatccctcc ggcaagggcc aagataaatg
     1381 gaacagttac tggaccagta gtcactcccc ccgaatcaaa agcaatcggc ataaattcgg
     1441 aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc tatagccatc aattgataat
     1501 aggatagctt gaatacgacc cgaagcagcg caaaggctag aaatactcca gtgccgacgc
     1561 tgacgatccc aatcagcaac actctgccaa tctctccgtc agatatatca tatacttgtt
     1621 gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac gatcccgata gcaaatccca
     1681 aactgaccaa aacggtcaaa cttttctttt tgatcatata tttccctact agatctccga
     1741 cttccatcat agaataatct gctccaaaca aaaagagcgt cattccgatc atcatgattg
     1801 ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa cggtgcaaaa acaaacgtta
     1861 aaatgacaat caggacagtc atcggcaata tagcagcaat cacttcttta aaatttttta
     1921 gcatgtatcc tcctccttta ctctttaagt ttaccttaaa atgaaagagt tttcgagcac
     1981 aaacttaaaa cgttatcatt taatttttct caacataaaa taacacacgt cccaaaagta
     2041 tctttttact tggaaaaatg actttgaagg aacgcgctta ttgtgttttt ttattttaca
     2101 gagccgatca ttccttgaac gaattgcttt tgcaaaacaa aaaatacgat ggcagttggt
     2161 aaagtagcga tgactaatcc agccatgatc attccatagt ctggtgtata ggaagcaccc
     2221 atagcagata aaacaagcgg aaccgtccgt ttatcaggag attgcaaagc tactaagggc
     2281 cataaataat tgttccagct actcatgaat gtgatgattg cagccgcggc ataggtattt
     2341 ttagccgtcg gcatatacac tctaaaaaaa atacttagtt cttttagacc gtctaatcga
     2401 gcggcttcta ctagatcttt tggaaaagat ttaacatttt gtcgaaagaa gaagatcaaa
     2461 aaagcagtgc taacagatgg caggatgaca accaagaaac tgttgattcc caaaaccgtt
     2521 ccgttcaatt gtgaaaacat tcgatataaa gggatcatca atgccgcaaa aggaaccatc
     2581 atcgatagca gtagaatatt gaaaattagg tcttttcgtt tactttgata aatctcaaat
     2641 ccatatcctg ccattgacga aatcagcaat gcaaacacag tggtcaccaa agcgatcatc
     2701 gctgaattcc aaagggaacg agtaaaattc agctcattgg aaaacaaatt ttgaaaattg
     2761 atgatcaagt tactgccaat ctttattttt cccgatgtga tatccacggg tgtattcgtc
     2821 attcccaaaa ccatccagac aaaagggaaa atggaaataa atgataggat gcttaatgct
     2881 gtatacttta atactgaccc aattgtcgaa cgaagttcta atgcttgttt tttgacgttg
     2941 gggcgttcga ttgtttcttt ttctaccata tttttcatcg actttctcct cctactttcg
     3001 tttggattat cgacaatatc acaataaagg aaacaataac aaaggaaact gctgccgcat
     3061 atccaaaatt aggcgtgaat ttataactca aattatagat gtattgagat agggtggtcg
     3121 ttgcatttgc tggtcccccg ccagtaatat tttgtacttc atcaaaaagc tgcaatgtcc
     3181 cgatcgtcga agtaatggaa gtaaacaaaa tgattggttt taaatttggg atcgtcactt
     3241 tgataaactg ctgccattta gaagcaccgt caatagatgc tgcttcataa atctccgaat
     3301 caatattttg catgcctgaa aggaaaaaaa tcatattgta tccagtccaa cgccaagtga
     3361 tcgaaagaat gatcagaact ctcgcccaaa ttggatgagt caaccagcca atcggtgtgt
     3421 caatcatacc aatgcccaac aacaagttgt ttacaaggcc agattgagaa aatagacttt
     3481 tcatgatcag agaataactg accatcgaag tgatacatgg aagaaagata gcggttcgaa
     3541 acaacccttt atatttcaac tttttatcat ttaaaaggtt ggaaataaaa agtgctagaa
     3601 acaacatgat cggtacttgt acaagtaaat acaggaatgt attaaaaaaa gcttttttaa
     3661 aagttgcatc tgtcaacatc cgctgataat tacctaaccc gttaaatgtc atattgacag
     3721 ggggaccact ttgaaaagac gtgatcaaag ccaaaaccat cggaataaaa accatgattg
     3781 atatcaaaat gactggcaag attaaaaaag catgcccgtt tttgaataaa tctttctgct
     3841 tcattctttt ccctccaatt ctatcattga ttatctacat gaaaagacaa gaaagacaat
     3901 atctgtaagg cagacgtgat cttttttgtc ttttcgtttg tctgatgaaa aagcgaagaa
     3961 attcactttt ttaccaccga tcaattagcc aattgtgctt ctacttgttt ttgtgtatct
     4021 gctagtacct tgtctgtctc ttctccattg atgattcgat gaagagcttc agctacgact
     4081 gcttcaattg cataggtctc atgtccgtaa ttcacttctg gtatttcaga tgtccatgtt
     4141 gaaaaatcgt caaagatctt ttgaccactg taaaattcag aagcttcttg ataagcttct
     4201 tgttcattcg cgctaagcat cgtagatact agtccgattt ctttcgcaag agtgcccatc
     4261 agttcttcat tcgaagcaaa cgttgcttct aggaaatctt tcgctgaatc ttctccagct
     4321 actcctttga tcacatacca gccagcgccg ccaaggttag aagcttgcgc tttttgcaga
     4381 ttttctggaa gtgcaggtat tggtgcaatc ttccattttc cagattggtt ttctgctcct
     4441 tggatcgttg aagaatacca agcgccagtt gggctagaag ctactgctcc tgattgtatc
     4501 gcagttactc cagcattcca gtcagaggtt tgttcgacta gatcttcttt caaaagagtg
     4561 gcaaacaatt caagaccata ctttaaagat gcattgtctt ggatcgtgac tgtctcccca
     4621 tcttcagatg tataccattc tcctgcctgt tgcataatca tccttactcg accaaggtca
     4681 gaaggattga cttcagcgat tttctttccc gtcttttctt tgacatctcg cgctacttga
     4741 atatactcgt cccacttcag attctccata tcctcttctg tataacctgc ttctgccatc
     4801 agatccgtac gatagaagtt tgctgttacg cccgaatcga aaggaacacc gtatatttta
     4861 tctcctactt tgtttaccgc aaatttatac ggtgcaaaat gatcttcctt tacaatagca
     4921 gacaaattag cgaatgcatc tggataagaa gtcaaatagc cttgtatcct ataatcttca
     4981 atcaagacga tattgggcaa tccttctgta ttcccgctcg ctaaagcggt gttcagtttc
     5041 tgaacgatat catcctgaga catcgtaaca acttctatct ctgtttcttc gttctcataa
     5101 actttttttg cttcattcac tgctttgata ttgaatgtct catcccacgc ccagacagtg
     5161 actttttctt ctttgtctgc tgtactacta gcacttccgc atcctgttaa gaaacctgat
     5221 gctgccgcta aacaaacgac tccagcaatt aactttctga tgttcatttt tatccgctcc
     5281 ttttccatcc attagttgtt tacgtttgct acattcatca taccaaaatt tgaaaacggt
     5341 ttctttatta ttcagatact tc
//
LOCUS       DAHEYX010000175         5348 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHEYX010000175 DAHEYX010000000
VERSION     DAHEYX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.176"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(24..578)
                     /locus_tag="MB219_002237"
     CDS             complement(24..578)
                     /locus_tag="MB219_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCC8427939.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            831..1106
                     /gene="rpsP"
                     /locus_tag="MB219_002238"
     CDS             831..1106
                     /gene="rpsP"
                     /locus_tag="MB219_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCC8427940.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            1118..1369
                     /locus_tag="MB219_002239"
     CDS             1118..1369
                     /locus_tag="MB219_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCC8427941.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            1494..2285
                     /locus_tag="MB219_002240"
     CDS             1494..2285
                     /locus_tag="MB219_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427942.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            2455..2976
                     /gene="rimM"
                     /locus_tag="MB219_002241"
     CDS             2455..2976
                     /gene="rimM"
                     /locus_tag="MB219_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCC8427943.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2966..3727
                     /gene="trmD"
                     /locus_tag="MB219_002242"
     CDS             2966..3727
                     /gene="trmD"
                     /locus_tag="MB219_002242"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCC8427944.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3863..4210
                     /gene="rplS"
                     /locus_tag="MB219_002243"
     CDS             3863..4210
                     /gene="rplS"
                     /locus_tag="MB219_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCC8427945.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            4468..4761
                     /locus_tag="MB219_002244"
     CDS             4468..4761
                     /locus_tag="MB219_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCC8427946.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1861 a          946 c         1036 g         1505 t
ORIGIN      
        1 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
       61 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
      121 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
      181 acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
      241 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
      301 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
      361 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
      421 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
      481 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
      541 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
      601 atctataata atcttacaaa agataagtaa ctggagcaaa taaaaacact tactgacaaa
      661 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
      721 tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
      781 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
      841 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
      901 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
      961 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
     1021 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
     1081 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
     1141 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
     1201 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
     1261 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
     1321 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
     1381 gtactcgaat tttcgagtgc agcttttttg ttttttttat taattgaaga aatgattatt
     1441 tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag ccaatgagaa
     1501 agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg gttactcaag
     1561 aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa
     1621 gtagtaccaa aacggcttcc accactgaac aaacaatggc tacctcaaat aatgagaaaa
     1681 agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct ctagatgtac
     1741 cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt tattctattt
     1801 tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat caagaaacgc
     1861 ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca atccaagttt
     1921 tgcagccatt tgagatcgac acaaatggtc agcaagtgga cttaggttct cgaatcacag
     1981 ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga aactggcgta
     2041 ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct aaagaaatcg
     2101 tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa attactgttg
     2161 atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct
     2221 atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct atgaagaggt
     2281 tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag gaagtaatca
     2341 aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga aaactagaat
     2401 tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata taagttgact
     2461 gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt
     2521 atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgct aacactattc
     2581 caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt
     2641 gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata tcgggatggc
     2701 atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt ctattaccat
     2761 gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt aaaagaaatc
     2821 ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg
     2881 cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta
     2941 gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat ttccaagaat
     3001 gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag gattattaga
     3061 tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag tagatgatta
     3121 tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg ataacatcca
     3181 agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg acccagctgg
     3241 taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact tagtattcat
     3301 ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag atgaagtttc
     3361 cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga tcgatgcaac
     3421 agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg attcttattc
     3481 aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag ggatgaaagt
     3541 accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat taaaagaatc
     3601 actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat ggacacctca
     3661 aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc
     3721 caagtgactt tttctaaaat cttctttaca gtaggttgat aatatgttag tattttatgg
     3781 tgagtgcaaa agcacttaac tattacgata ttccgctgtg agaaaagctc ataagaatat
     3841 ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa aagaacaatt
     3901 acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg caaaagttgt
     3961 tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc
     4021 tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac
     4081 attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg
     4141 tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat
     4201 ccgtcgttaa tcattgttat cagactgaga aagaaccctt ggtattcaag ggttcttttt
     4261 tcttagcatg taatttgaac accctgatcc agttttttct ttaattattg gtaggggaga
     4321 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
     4381 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
     4441 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaagatg
     4501 aaagttttag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
     4561 aagaaataca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
     4621 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
     4681 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
     4741 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
     4801 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
     4861 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
     4921 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
     4981 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
     5041 ctaaaattag attttggaca gatgcccttt aaaaagataa caagatttcg ttcttgaaac
     5101 aatatatatg cttttattcc aatgaggttg tgaaaacagt attttgtcct gagcgttaga
     5161 aaattgaaaa tggggaatag caactcgtac tttaccattt tttaagtgaa tacaagatca
     5221 gctaattgaa caccgtttat tcgtgatgag ggacacaaca ctgcagtcga cacatttctc
     5281 ttttatgttc aaagataaca aatatttaaa acaaagaaaa atagtagata atcccgtcct
     5341 aactcttt
//
LOCUS       DAHEYX010000176         5256 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHEYX010000176 DAHEYX010000000
VERSION     DAHEYX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.177"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(180..725)
                     /locus_tag="MB219_002245"
     CDS             complement(180..725)
                     /locus_tag="MB219_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCC8427947.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEARLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(748..1233)
                     /locus_tag="MB219_002246"
     CDS             complement(748..1233)
                     /locus_tag="MB219_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCC8427948.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(1223..2035)
                     /locus_tag="MB219_002247"
     CDS             complement(1223..2035)
                     /locus_tag="MB219_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCC8427949.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(2174..3706)
                     /locus_tag="MB219_002248"
     CDS             complement(2174..3706)
                     /locus_tag="MB219_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCC8427950.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRACQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPDLSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEVHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQNAVFLQVYGQSECGPMILKAHTLESLKISD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(3815..4846)
                     /locus_tag="MB219_002249"
     CDS             complement(3815..4846)
                     /locus_tag="MB219_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8427951.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFRTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFELTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVQVSSI"
BASE COUNT         1620 a         1035 c          924 g         1677 t
ORIGIN      
        1 cagatcagtc ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag
       61 gcatactagc accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta
      121 aagttcatta gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt
      181 caataattcg ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga
      241 gcttgtccaa gggttttcaa acgaggtctg acaggtactt ggacatgttt gatatgcata
      301 ccgattgatg tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca
      361 ggatcattga actggtcaaa agcagcaaca gaacacgcac ctccagcatg tagtgcagga
      421 acaacagaga caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct
      481 cggttcaaat gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct
      541 tttaacgtac ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct
      601 ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagcgtgcc
      661 tcagccaata tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac
      721 ttcattttct aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag
      781 acttgcagtt ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg
      841 tatagaagcc actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat
      901 gatcataact aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct
      961 gaaagaacca gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg
     1021 cggatatcct gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc
     1081 cgttggacca taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc
     1141 attcgtagcc ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag
     1201 catagcatag atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc
     1261 cagaccagac gcttccggta tgatcgttca tcagcacacc attagaaata caatgggcaa
     1321 caaatttttt actttctgct actgctttgg taaagtctgc ccctaacaca agattcgctg
     1381 ttattgcaga agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat
     1441 gagaaaatac ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca
     1501 tccttgtgcc gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga
     1561 gatggatcat ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg
     1621 ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat
     1681 acaattgatt gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg
     1741 ggatattttg ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgagttca
     1801 ataacccaat tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt
     1861 taagcaattc caaagatata gattccagca taaatcgctc attaatatct agtgccccta
     1921 tagaagtcaa ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt
     1981 ggattcctcc accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat
     2041 tttcctccat gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat
     2101 atttttatct catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc
     2161 tttccttaaa tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca
     2221 ctttcattgt agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc
     2281 gaggaagttc atggacttgc tcccaccatc gattccaatc catttctttg tcaggagcta
     2341 aagcaataat cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa
     2401 aatctaacga atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca
     2461 aatccacttg ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc
     2521 cgctatccca ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag
     2581 tcaaagcccg tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat
     2641 tcccaactga atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc
     2701 ttgcatcact tattttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt
     2761 cactttggcc atatacttga agaaataccg cattctgtgc catcgacgtt cgcaaaaatg
     2821 tcttcattgt ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa
     2881 aagcttctgg atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct
     2941 ctagtgcaat tggccggtga acttccaaca tatgaacaac ttttgaactt tgtgcgtttg
     3001 ctaaaggcat cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg
     3061 aatgcactgg agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg
     3121 ttttttgcca tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga
     3181 taccagttgt accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt
     3241 gagaaacaga caaatcaggt tgttccatta aataaagaat aggaatctgt tttgattttg
     3301 ttccatttga aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt
     3361 cttctaaacg atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga
     3421 caggcactgc accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac
     3481 tcttataaat aataactttg tctccggatt tcactccaag atgagccaat tgacaagccc
     3541 gtttcaacac actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag
     3601 ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa
     3661 aattcgtaaa taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat
     3721 cttttgttaa gtaatacaca aattcaagat aatcgtaaca taatccttta tatagtaaca
     3781 tagtttattg tgaaaaaaag atcatacatt tgttttatat ggaagaaacc tgaacgaatg
     3841 ccgctaattc gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg
     3901 atgagaggta tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt
     3961 ctgcataata acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa
     4021 ttttgctcaa aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa
     4081 aaacaaaaga attttcgtcc gtattttgtg ctgttaactc aaaatagttc tccagaaacg
     4141 gcatcgcttc ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa
     4201 gaatctgctt ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta
     4261 atccgcaata cagaagaatg gcagtaattg caaggttcct cggtcgattc ttttcttcaa
     4321 agctagcttc tctgcttgtt cgaaagcctt caatcgggat aaacaaatgc tcaaaatctt
     4381 tgaaaagttc tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac
     4441 ccaaaagaga aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat
     4501 ttaatgaata ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt
     4561 cttggttcgc tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct
     4621 gataatcttg tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt
     4681 ttctcaaata atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg
     4741 aaagggaaga acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac
     4801 gttgctttaa cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc
     4861 aatagtgttc attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct
     4921 tgatcttagg aagcaaattt ttaactgtct tttaaaagta ctagctaaaa gatttgaagt
     4981 aagaagatac atttttagtt attttattag tgcagtcatt cttttgatat tttaattgat
     5041 ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt ggttcgttct
     5101 aatggcagga ggctttttct tttatttccg aaaaattaga aaacattttt tcagaatcga
     5161 ttggctctcc atttattcaa taatagtttg tctaacgata ttgttgggat aattcttaaa
     5221 taagtattag aaatagttgg tacttcatta ctttac
//
LOCUS       DAHEYX010000177         5240 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHEYX010000177 DAHEYX010000000
VERSION     DAHEYX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.178"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            1..357
                     /locus_tag="MB219_002250"
     CDS             1..357
                     /locus_tag="MB219_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HCC8427952.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="MB219_002251"
     CDS             373..723
                     /locus_tag="MB219_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HCC8427953.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="MB219_002252"
     CDS             752..1531
                     /locus_tag="MB219_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HCC8427954.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="MB219_002253"
     CDS             1542..2219
                     /locus_tag="MB219_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HCC8427955.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="MB219_002254"
     CDS             2238..3344
                     /locus_tag="MB219_002254"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HCC8427956.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="MB219_002255"
     CDS             3334..4425
                     /locus_tag="MB219_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HCC8427957.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="MB219_002256"
     CDS             4438..5178
                     /locus_tag="MB219_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HCC8427958.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1676 a          861 c         1251 g         1452 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt
//
LOCUS       DAHEYX010000178         5218 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHEYX010000178 DAHEYX010000000
VERSION     DAHEYX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.179"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            42..713
                     /locus_tag="MB219_002257"
     CDS             42..713
                     /locus_tag="MB219_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427959.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            772..1212
                     /locus_tag="MB219_002258"
     CDS             772..1212
                     /locus_tag="MB219_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427960.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1224..1892
                     /locus_tag="MB219_002259"
     CDS             1224..1892
                     /locus_tag="MB219_002259"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCC8427961.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1956..2579
                     /locus_tag="MB219_002260"
     CDS             1956..2579
                     /locus_tag="MB219_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCC8427962.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2635..3021
                     /locus_tag="MB219_002261"
     CDS             2635..3021
                     /locus_tag="MB219_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCC8427963.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3234..4625
                     /locus_tag="MB219_002262"
     CDS             3234..4625
                     /locus_tag="MB219_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCC8427964.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAVAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1736 a          861 c         1097 g         1524 t
ORIGIN      
        1 aaatatgcta ttttaaaaat gatgaaaaaa gggggagaaa aatgaatttt ttagttgttt
       61 taaaagatac tctgattgag cgatctacat ttctatatgt tcaattgcag gaaaataaaa
      121 cattgctgca ttttttacca gagttccatc tagaggggct aacattagga gaaatctatc
      181 aagcagaagg gctgtcggct gttttgcact tttttaaggt agaattggaa ttaccagtaa
      241 aagattatat cgagctttct cttgaatctt gggatcgagc agtggttgaa ctgtttccag
      301 aaggtttaat gatcgataca aacgatggga agatccatct gactgcagca gaactgcaaa
      361 aacaaatgcg ttatcaaata gatgacgaaa attctttttc tatttttaga cgtcagcaaa
      421 agattctaaa aagcttttta aaagtgatca gtaaaaaacg gaatctgatc aaaacgacac
      481 agctgttgaa aaaatatcca ggtctgcttc acacgtctat tcaatttcct caattgatta
      541 cgatgatcca tcgttttata cgtatgcaac agctaccatc taagaagctg tttcttccat
      601 tgacggacac atttcgagta gtcaacagag aagataaaaa gaaagtgatt gtgattgact
      661 ttaccagaaa tcggaatgtc cttcaccaag ttgcaatgga aaaggccaat taaggctttt
      721 tctttttgga tttttttgtc ataataaata ttagatgatg gaggagataa tatgatgcaa
      781 acatggttag aaaggctaat acgatttcag cagaactatc acaaacttat gcagggaaga
      841 tatgcgaaat ttgatcagtt gaatcgcggt ctattgatcg ctgcgcttgt ctttagtttg
      901 ctaagtcgtt ggttgcccta tagaatcgga caattgttgt tcatcgtctt tttcggatgg
      961 atgatatttc gtttcctatc aaaaaaaatc taccccagac taaatgagaa tcaacgttat
     1021 ttgaaatatc ttgatagagt caaagaaaaa tatcatcaga taaaaaacaa aaagcaagtc
     1081 aaaatggatc gaaaaagtta tacttttttt gagtgcccga attgtcatca aaagcagcgt
     1141 gcacctaaag gaaaaggacg aatacgtgtg acatgcaaaa catgtggtat aaaattcgaa
     1201 acgaatgttt aagggagaaa agaatgaaat tagtattatt acgtcatggc gaaagcgaag
     1261 caaattttga aaattactgg acaggatggc tagatgtcgc cttaacagaa aaaggaaaag
     1321 aacaagcagc agttgccggt caaaaaatgc ttgaagccgg acttcaattc gatgccgttt
     1381 atacctctgt tttgaaacgt gcgattctta ccggacaagt cgcactagaa gaaatgggtc
     1441 agttatggct tcctataata aaaacatggc gtctaaatga acggcattat ggggatcttg
     1501 tgggtaaaaa caaagataag atgaaagaaa tatacggaaa agatcaagta aaaacatggc
     1561 gcagaggata tacggaagtt ccgccgttaa ccgcagaaaa tagatttgac cgcagatatg
     1621 atcacttgga tccgcatttg atcccatacg gagaaagtct ggaaatgaca gtgaatcgga
     1681 tcattccctt atggcaagat catctagcac caaaactaaa agatcggcaa gatttactag
     1741 ttgtgggtca tggtaatagc atccgagcac tgacaaaata tttagaagat gttcctgaag
     1801 atcaaatgga tacgatcgat atcccaaatg cacaaccaat ccagtataca ttagcagatg
     1861 atttgacaat tctggacaaa aaaatacttt aaagaacacc tttatctaaa agtgagagat
     1921 agaaaaaaga agcaaaggaa tggaacaaaa ggaacatgaa aaattatata tgggactttg
     1981 atggtacatt gtttgatacg tatcccgcaa tggtagatgg tgcatggcaa gcactaaaag
     2041 attttggtat cagtatggac aaaaaagaaa tctacttcaa aatgaaaaag tattctacta
     2101 gttatttgat caatgaatca aacttgaatg ccagagagtt taatgaatta tttcaccgat
     2161 atgaaaaaga aagtacggaa gtttcccgtc catttccaga aacgaaacaa gtactggaaa
     2221 tgctaaaaga caacggcggt cgtcatttca tcctgaccca ccgtttgaca gaatcaacat
     2281 ggggcctgtt aaaagaacat cgattggcac atctgattga ggaagtagta gggatcgatc
     2341 aggactttcc tcgtaaacct gatccagcat ctttaaatta tttgatcgat acttttcatt
     2401 tagaaagaac agacacgatg atgatcggcg atcgtcgttt agatatcgaa gcagggaaaa
     2461 atgcaggagt cgctacttgt ctttacgaca tcgatcattt tctgggtgag atcccggcag
     2521 attatgtggt aggaaatctg aatgaaatcc ttaccttgca gagcatcatt aaaaagtagt
     2581 atcccctttc aaaggagata tgttataatt ttcataaagg agtgataaaa ttatatggaa
     2641 atcagaagac aaaaagaagt tgtagaagca tttcattatg atatgagatt gccgaaccaa
     2701 gaagtagaaa cagatttgaa agtaggcttc tcgcctttaa aagaaacgat cgaaaactat
     2761 ccgaaagaac atagcatcgt aggagcacgc ttagagttcc gtttagtgtt tgatacatat
     2821 gttttatctg gaagtgtgag tcagatcaat catatcctca atcggaaaat tgaaggtcaa
     2881 aatgatgtca cacaagaaga agtggacgag ctagtagcac cgttattcga tatcgtacgc
     2941 cgaatgtctt atgaggtgac agagatcgcc ttagatcagc ctggggtaaa attgaatttt
     3001 caatcagaaa aacaagaata gacagtaaaa acatacctgc gatagattaa agctatcaca
     3061 ggtatgtttt tttattacta attgtaagca aaaagaatgt catttcgata aaaatcgtct
     3121 aaaatataaa agtcactgaa aaaaattttt tcaggaggat ggttgaagaa tcattctcgg
     3181 gaacctttac ttccttcgat ggacttgtca ttaataatat gaggaggaga aaaatgacaa
     3241 cactcatcac agttttagtg gttctagttt ttgcggcatt attatatgtg ttctatcgta
     3301 tgcaaaagcg ccatacaaaa ttttcaacac gcgtctttac agcattagga gttggtatcg
     3361 tctttggcgg tctgcttcaa ttgatctttg gtgtagacga caaagtcgtt actcaatcaa
     3421 tggaatggat cgggattgta ggtcaaggat atgtatcatt cttgcaaatg ctagttatgc
     3481 ctttagtgtt tatttcgatc attggtgcat tcacaaaaat gaaagtttct gaaaagctag
     3541 ggaaaattag tgcgactgta ttgacttctt tacttggaac aacagctgtt gcagcattga
     3601 tcggaattgg ttcaacaatg ctgttcggac tccaaggagc taaattcaca caaggagctg
     3661 cagaaacagc acgaatctct gaactggcaa cacgtcagga aacagttgaa aacttatcga
     3721 ttccacagca gattttatcc tttatcccta aaaacgtttt tgctgattta gcaggtagtc
     3781 gtgcaacaag tacgattgga gtcgttattt tcgcggcatt tgtcggaatc gcttatctag
     3841 gcgtgaaaag aaaagcgcca aaagaagcag cattcttttc agatttagtc gacagtctgt
     3901 ataaaatcat tatgcggatc gtgacgcttg ttttgcgttt gacaccttat ggagtatttg
     3961 ctttgatgac aaacgttttg gcaacaagtg attttgcagc tatcgtcaat ttaggtaaat
     4021 ttgtcgttgc ttcttacgta gcattgcttg ctgttttgat tgtccactca ctgattttga
     4081 ttggtgcaaa agtcaatcca atcaactatt tcaaaaaagc ctttccggta ttgagctttg
     4141 cgtttacttc acgttcaagt gctggagctt tgccattgaa tatcgagact cagacaaaag
     4201 ctttaggagt agatgatgca acagctaatt tctcagcaag ttttggtttg tctattggac
     4261 aaaacggctg tgctggcgtt tatccagcta tgttggcaac gatcgtcgca ccagcagctg
     4321 gagtgaatgt ctttagtttg gaatttattt tgatgcttgt ggcaattgta acaatcagtt
     4381 cttttggtgt agctggagtc ggtgggggcg caacatttgc ttcactgatc gttttagggg
     4441 caatgaactt gccagtagca atcgttgggt tagttatctc tgttgaacca ttaatcgata
     4501 tggcacgtac agcagtaaac gtcagcggta gcatggtagc tggtatcgtg acaagtgcac
     4561 gcatcaaaga tttagatcgc gatgtcatca atgatgattc taaagtgatc gatgtgaatg
     4621 catagtcaaa aagaggttgt gatagaagtg gtcagcttta agaaataaga agaagtttcc
     4681 taaaaatagt ttctcaaatt tttgtgaaat ttcggcttat ttccgagaag ctacttctga
     4741 aacaccgtga ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     4801 acaaaaatag ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt
     4861 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgtttc actctgagtc acaaagagca
     4921 atttcaaaaa atagcttctc atgtttgttg aatattgtgt cttgtgaccg aggagttgtc
     4981 aggagaacac cgtttaccag aggttgtgac ttgtttttag tcacgacctc tttttttatt
     5041 tattccatat aaatacacat tatttattcc gaaaagtgaa agcgcaaata acaaaaacaa
     5101 ccaacttatt tattattaaa gaaaagcgta tttaagtagg acgattctgt tgagagcttt
     5161 cgtagatttt cccattttcc tatggtaaac tgacaaagtt gagaatgtta gaacagat
//
LOCUS       DAHEYX010000179         5136 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHEYX010000179 DAHEYX010000000
VERSION     DAHEYX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.180"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            47..2341
                     /locus_tag="MB219_002263"
     CDS             47..2341
                     /locus_tag="MB219_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCC8427965.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            2334..2999
                     /gene="pgmB"
                     /locus_tag="MB219_002264"
     CDS             2334..2999
                     /gene="pgmB"
                     /locus_tag="MB219_002264"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCC8427966.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            2992..3999
                     /locus_tag="MB219_002265"
     CDS             2992..3999
                     /locus_tag="MB219_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCC8427967.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            4021..5040
                     /locus_tag="MB219_002266"
     CDS             4021..5040
                     /locus_tag="MB219_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8427968.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         1672 a         1058 c         1033 g         1373 t
ORIGIN      
        1 ttgcgtttat tatgaacgta taaaacagct cattaggagg agaaaaatga aacagatcaa
       61 acgactattt gctattgatc catggaaaat cagcacgaca caattagata aaaaaaatct
      121 tcgactgcaa gaatctttaa caagtattgg aaacggttat atggggatgc gcgggaactt
      181 tgaagaaact tactcagggg accaccatca aggtacttat ttggcgggtg tctggtatcc
      241 cgataagaca cgtgtcggct ggtggaaaaa tggttatcca gaatactttg gaaaagtgat
      301 caacgccttg aactttatcg cgatagatat ttcgatcaac gatcagctga tcgacttggc
      361 ttctttggaa ccagaagact tttactggga attagatatg aaaaatggtg tattatctcg
      421 tacattcaca atcactactt ctactaataa agtacgcatt tccttcgaac gttttctgag
      481 cattgtcaaa aaagaatcag cttatatcta tttaactata gaaatgcttg aaggtagcgg
      541 aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca gcaactatga
      601 tgaacatttt tggaatgaga ttgatcgtgg aactatagaa gaaatcggct atttgacgac
      661 taaaactatt ccaaacaact ttgggattga acaatttact gttactgcag caatgcgcca
      721 tttcgttaat ggaaaatcag tcatccctga ctatcacgaa gatactttag cattacatgg
      781 ttgcttgtct gcagaattat ctcaaggtga gacatggtca gtggaaaaag aaattatcgt
      841 tcttactagt agagatattc cagaagcgca gcaagcagaa aaagctgtct cagaattaac
      901 gaaccagaca aacaattata tccaagcaaa agaagagcaa acagcggctt gggcagaacg
      961 atggaagctt gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag gtattcgatt
     1021 caatttgttc caactattct ctacttacta cggcgaagat gaacgattga atatcggccc
     1081 caaaggattc acaggagaaa aatacggcgg agccacttac tgggatacag aagcgtatgc
     1141 tgtccctctt tatcttgctt tagccaaacc tgaagtaact aagaatctgc tgaaatatcg
     1201 gcataatcaa ctgccgcaag caatccacaa cgctcagcaa caaggattaa aaggcgcgct
     1261 ttatcctatg gtcaccttca ctggtgtcga atgccataac gagtgggaaa tcacattcga
     1321 ggagattcac cgaaatggcg cgattgccta tgcaatctac aattatacga attacaccgg
     1381 cgatacggaa tatttgaaaa atgaaggact ggaagtttta gcagaaatcg cacgcttctg
     1441 ggcagaccgt gtccattttt caaaacgagc aaataaatac atgatccacg gtgtgaccgg
     1501 cccaaacgaa tatgagaaca atatcaataa caattggtat acaaatacga tcgcagcttg
     1561 ggtcttgcgt tacacgcgag aaaattattt aacttatcaa aaagaagctg ctatcactat
     1621 cagcgaagaa gaactagcca aatggaaaga tatcgcagag aacatgtact tcccagttga
     1681 cgaagaatta ggaatctttg tccagcatga cacattctta gataaagatc tcatgcctgt
     1741 atccgatatt cccacaagcg atcttcctct gaatcagcat tggtcttggg acaaaatcct
     1801 tagatcttgc ttcatcaagc aggcagatgt actacagggg atttatttct tcaatgatac
     1861 ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat gaaccaatga ctgtccatga
     1921 gtcttccctt tcgccaagta tccattcgat tttagcagca gaattaggaa tggaaagcaa
     1981 agctgtagaa atgtaccagc gaacagctcg cttggatttg gataattaca ataacgacac
     2041 agaagacggc ttgcacatca cttccatgac tggtagctgg ttgtcgattg tccaaggatt
     2101 tgctcaaatg aaaactgccc atgatcagtt gagattcgca ccatttctgc caaaaacttg
     2161 gaaagcttat tctttccata tcaattatcg cggacgttta ttgttcatct cagtcacaga
     2221 tgaacaagtt actttcgagt tattagaagg ttctcctcta gaaatttacg tctatgaaca
     2281 gtcttattct ttagcggatc gtctagaaat aacgctaaga aaggaggaag caaatgttta
     2341 aaggggtatt gtttgattta gacggtgtca ttaccgatac tgctgaattc cattatcgtg
     2401 cttggcgaaa gcttggtcag gaaatcggca ttacgatcga ccacacattc aacgaacagc
     2461 taaaaggcgt cagccgagaa gactcactct ctttgctttt agcttatggc ggaaaagaac
     2521 actctttttc aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat tattatttag
     2581 agatgatcca gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt
     2641 ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac gggccattcc
     2701 tacttgaaaa aatgggtctt acaccttact ttgatgctat tgccgacccg gcagaagcaa
     2761 caaacggaaa acctgcgcca gatatcttcc tccttgcagc aaaagctgtc ggactaaaag
     2821 cagaagaatg tatcgggatc gaagacgcgc aagccggtat ccaagccatc ctctcaagtg
     2881 gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc
     2941 ctgatacgac ttttcttacg atcgattact tgaaagaaaa gtggcatgac catggataat
     3001 ccagagatct ccgtcgtacc agttccagag actgaaataa agaaaatcac attgaagaac
     3061 gactacttag aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca
     3121 gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga agatgttcta
     3181 acagacaaaa gcttttttgg agcaactgtc ggacctgtag ccggaaggat ccgggatgct
     3241 tcttggggtg agcatagatt ggagaaaaac tgcggatcac accatatcca cggtggcacg
     3301 aaaggctggt ctttccaatt ttgggaagta gaagttttca aaactccatc atcaataggt
     3361 gtggtctttt acttaaaaga tgaattttct acctatccag gaccaatcac tgctacgatc
     3421 acttaccgtt taacaaaaaa cgaattagag atgacaacct ctgctagtag ccttgttgaa
     3481 accatctgca atccgaccaa ccatgcctac ttcaaccttt caggaaatgg aacaagagac
     3541 atctatgaac atcagttaac ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa
     3601 ctgcctactg gaaattggac aaaaaaagaa aaactgccaa tcgactttag aaaatcccca
     3661 acactgcaag agatccttgc atgttatcca gatggactag atgatgtatt tcttttgcat
     3721 catcctaggc tttccaaaac ttccttacaa ttattcgaga agcattctgg tcgccaaatg
     3781 actatagcaa cttctaataa aagtatggta ttattttcta cgactggatt cgaagctgat
     3841 ttctctgtca atggaaaaca gatgcattca aactacggat tagcaatcga accgcaagaa
     3901 atccctgata tcgttcattt ccccaaattt ggatcgatca acctccatcc tggtcaagaa
     3961 cggatcagtc agacgattta tcggttctct gcacaataac aagagaaagg tgtttttatc
     4021 atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct
     4081 cgtgtcatca acgatcatcc tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt
     4141 atggatgaac taggttatgt tcctaacatg actgcccgca atctaggaaa acgcatttcc
     4201 agtgccatcg gcgtaatctt accccctctt gattcaaaag agcggatcgg taacccattt
     4261 tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga attatgatat gactacagca
     4321 atcgctactg ctaaaaattt tgaattgctg ttagagaacg tccagcgtat gcatttacaa
     4381 aaacaagtag atggttttat tctagtgtat tcagatagtc aagatactgt cattgattat
     4441 ttataccaaa accacatccc tttcactttg atcggacagc cttatcatca tgagaacgaa
     4501 atcgtttatg tagataatga taaccagctt ctaggtaaac aagcaacaga atttttgatc
     4561 cagaatggac ataagaatat cctgtttgtg acaaacacga ctcatgaaag tttgtatttc
     4621 gaacgatatt tcggttatca aaaagcaatg atgatggctg gtcttcctgc gttatccagt
     4681 gtagtactag agcatccgga ggattatctg tcctttgaga aaacgttgaa agaaaacgaa
     4741 gccacagcat tggtggtcat tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa
     4801 atgtatggct atgtcattcc agagacactc tccgtcatta gcttcaacaa ctcgatcttc
     4861 tcaacattaa cacaccctta ccttacaagt atcgatatcg atatctccga attaggacgt
     4921 gtcgctatgc aaaagctgcg cgaattgatc catgaagaat tgagcagtgg cgtacgtttg
     4981 gtcatccctc atcgcttgat caaaagagag acagtcgttc ctttagaaaa cccatactaa
     5041 atagctgact cactaaatac gctgaccatt tatcctatgg tcagcttttt atgtaaaaga
     5101 agatactact tctaacacat tgtttatctg taaaaa
//
LOCUS       DAHEYX010000180         5075 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHEYX010000180 DAHEYX010000000
VERSION     DAHEYX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.181"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            60..977
                     /locus_tag="MB219_002267"
     CDS             60..977
                     /locus_tag="MB219_002267"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCC8427969.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(1355..2266)
                     /locus_tag="MB219_002268"
     CDS             complement(1355..2266)
                     /locus_tag="MB219_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCC8427970.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(2667..3245)
                     /locus_tag="MB219_002269"
     CDS             complement(2667..3245)
                     /locus_tag="MB219_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8427971.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(3265..3933)
                     /locus_tag="MB219_002270"
     CDS             complement(3265..3933)
                     /locus_tag="MB219_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8427972.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(3948..5018)
                     /locus_tag="MB219_002271"
     CDS             complement(3948..5018)
                     /locus_tag="MB219_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8427973.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         1531 a         1054 c          839 g         1651 t
ORIGIN      
        1 agaaaacgaa aagtcgtgag ttgcggtata gttttatgta taaaaacgag gagaatttta
       61 tgagcaacgt cattatcaaa gcaacaaacg accaacttag tcaaatgaaa gcatactatc
      121 gctcttattt gttaaataaa cagattcctt atactagctt tgctgcaaaa aaagacggga
      181 cgaccattac cgcttataca tccggaaaag tgatgtttca aggtacgaat gcagcaatag
      241 aagctgataa atggggggag gcacaggata aacaagccaa gccagcaaaa acagcctctt
      301 ctttgccaaa aaatatcagt caattatccg ttttaggtag tgatgaggtc ggaaatggca
      361 gctatttcgg tccgctaaca gtatgtgctg cttatgtgaa acgtgatcag cttgcagaat
      421 tacgtaaact tggtgttaga gattcaaaag agttaaaaga tagccaaatc atccagcttg
      481 ctgacatctt aaaaaaaacc atcccctata aactgcttgt cttatctcca gaaaaataca
      541 atgagatcca accaaaatac aatgccgttc ggatgaaagt cgctttgcac aatcaagcga
      601 tccatttgct gctaaaaaaa atcgagccag aaaaaccaga agcgattttg atcgatcagt
      661 ttacgccgga agcaaatttt aaaaaatatg cgcgtttaga aaaaaatcaa ttacaagaaa
      721 aactgtactt cgttactaaa ggagaacaat atcatttagc tgttgctgct gcttctatca
      781 tcagtcgtgc cagctttttg gaagagttag acaaagcatc agctgaagct ggaatcaccc
      841 ttccgtcagg agctggaacg aaatctgacc aagtagcggc aaaactattg gagaaaggcg
      901 gcatgcctat gcttgaaaaa tatgccaaac ttcattttgc caatacagaa aaagctttga
      961 agctactaaa aaaataatgg aactgtgaca aaagtcatgt cacagtccct tgttccgaat
     1021 aaacggtgtt caatcatctg atcctcggta ttagctcaac aaacggcaaa atatgaggaa
     1081 ctatttttcc gtttgtttct ctaatactca gaacaaaacg ttgatttcac aacctttttc
     1141 ttaaacggtg ttctcatgcc aactcctcgg tcacaagtca caatagtcat caaacatgag
     1201 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctct
     1261 tccttaaacg gtgtttaaaa gcttgcatct gcgataataa gcccggccaa acgaaaaata
     1321 tgaggagcta tttttgtttg gctgggctta tttattactg aaaataagtg gtcaatgctt
     1381 gtgtcaaact tgcagcaatc tgctgctgat aactgtctgt tactaattcc tttaaatcac
     1441 tatcattatt catataaccc atctcgatta aaatagaagg ttgaagattt tctcgaagaa
     1501 cttgatagtt cccagtggag attcctcttg aatccagagg cagatcagtg aaggagtcag
     1561 caattgtctg tgctaatgtt tcctcttgtc catagtaata atatgtcgtg attccagttg
     1621 cttcattcgc atattccgta gagtctgcgt gtaaactgat aaagacatct gccttttttt
     1681 cttcactgta gtcgcatata tctccaagct gtactaattc atctgtagta tgggtcaaat
     1741 aaaccgttgc tccagcatcc tctagcgcct tttttacttt tatggctgta cttaatgtta
     1801 tatctttttc ttctgtttgt tcatcattgc ttaatgcccc agtatcattt ccgccatgcc
     1861 caggatctaa tacaatcact gcatcagata gctcttccgg ttttgtttgt atattctttg
     1921 tgactttggt tccatcttcg agggctaaat atccagtata gccatcattt gtcttgatct
     1981 gataagcatt ttttgtttca gaaagaaact ccgccagctc tccgccgttt atagttgcaa
     2041 gtggggtagt tagcgagtca tctgtatata gtgtacggtc attttttccg attttgatta
     2101 atgtctcttg ttctgtatca tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac
     2161 tgctgaccgt ttgtccaact atcttttttt gaccaaaaac atctatttga aaacagataa
     2221 taatgataat gattgcaaat acaaaagctc caattatttt tttcattctt gactctcacc
     2281 tcgtagatcc agactaaggg ccaaaactta aaatcagctt aagtaagcaa aaaaggggct
     2341 gtaaaagata taaccgtaga aaataacatt actgctaaaa tgattttttt catttttggc
     2401 atatcctgaa atctcctttt gactattcgt ggtgattcat ctacccaata aatagtttcc
     2461 ttcattctac aaacaattaa tttcttcgtt attaaattaa aacacttcct ggaacaccga
     2521 cataataaat aaaaatatac cctgataatc atgagagaat aacaaatttg tatttatgaa
     2581 aaactaccta tataaagaag caagatataa cgatcttcgt catatcttgc ttcttcacct
     2641 aactattttc tccctatata acagctttac gcttctccta atacaccatt caaatataaa
     2701 tcgatcaatt gcgccacatt tagcggttct tccacaggtc ttttcgaccg agtgatgtaa
     2761 ggtgctaaca ctaagaaaaa atgccttgct gcgatttctt ttgaaatccc atttcttagc
     2821 acagattggt ttgctgaaaa gatttcttca aaaggttcac gataattttt cttccataga
     2881 aagaaaagat gatctcttgt ttcctttgaa agattgcttt caagatcgtg aagcatcaga
     2941 aacaaatcga ccagatgtgt ctcaatcaaa aactgcgcca tttttgtcaa ggttacttga
     3001 aatcccgttt cgcttgtttg aacgatttct ctcaaagctt ttccaacatc ttttaagtgg
     3061 ttttctaaaa ctgccgtata tagtttttct ttatcaccga aataatgata gagattgggc
     3121 tgggtgatcc caactttttg tgcgatttct cttgttgaag tgccgagata tcccttggac
     3181 ataaacaagt cagtcgcttc tttcaaaatt ttttgctgtg tatctttgtt gtatcgatgg
     3241 accatttttc cacctgcttt ttggttattc tttttctttc aatacaccat cttccatcat
     3301 aaatattcta tcgcatttgt caatcagccg tgtatcgtga gtaaccatga tcgttgcttt
     3361 atttttgtct tttgattctt ttgccaagat atcgactact tctcgtgcct tttctgaatc
     3421 caaactagca gttggttcat ctgctagaat aatggacgga tcattgtaca atgcacgagc
     3481 gattgcgacc ctttgccgtt ctccaccaga tagttcttct ggatacttgt tgatcaactt
     3541 atccacgcca agctgctcaa ataattcgtt tgcttctttt tgacgatgtt catgtttaat
     3601 cttatctact aataataact gcttttttac tgtcagaaaa ggaaccagat tggaagcttg
     3661 caaaataaag ccaatttctt taaaacgtag tttaacgcgt tcgctttctt tttgattact
     3721 gaattcttga tcattgataa atacagagcc actgctaggc gtctgcaatc cgcctgccaa
     3781 agtcaaaaaa gtacttttac ctgaaccaga aggaccgatg attgcaacaa attccccttt
     3841 atcgatactc atggttgttt ctttcaatgc tttaactact tggtcgccat cttggaaact
     3901 tttttctact tctttaaatt ctattgcttt catgttcttc ctccttctta tccaattgcc
     3961 ttcaacggat cgatttttac gattgtccga acagaaaata gtgcgcctaa cacagctact
     4021 actaacatca acaagctaat gacgccaaaa aacagccaat tgctttggaa tggaaccgca
     4081 tcaggtagaa caagggatgt tcccactgtt cctaatagac caagaagaat accaacaaat
     4141 gataaaatga atgtttgtgc cactacagaa cgcgcaataa atcctcctgt gatcccttgt
     4201 gctttcatta caccaaagat attaatcttt tgcatcgtca gtacataaat gaaaatcccg
     4261 atcacaatag ccgcaataac gatcaaaaat ccgatcataa atccaaaagt caatacttgc
     4321 gcattatatc caggtaattc attgatgaat ttagaaattt tgatctgttc taaatcttca
     4381 ggtaaattac tgattttacc tctgacaact attgcgttaa tccgtgcatt ttcacttgta
     4441 tcttcttttt cgaagcggat ttcttggtaa gcattgatgg ttgtatagag aactggtgag
     4501 acattgaatt tggcatgatc cgtaaagcca gttagtttaa acgtcttgtc actaccagat
     4561 aacttaactg tatctcctat ggctagacca tattcttctt tcaaactaat atcgcctact
     4621 gcctcatcat cgttatcaaa agcttttcct tcaacaagat tgggcatgat gaattgattt
     4681 ttatcgatac cgaagaagct aacattgatt tttccttctt ctgtactgtc tttactagtc
     4741 acgactccag gtgtttgggc aagatacgcc actttcccac cttcgacttc ttctgctatc
     4801 ttcttagtaa tcatggacat ccctagattt gaatttgcat ctttcgaaag cacaatcgaa
     4861 tctgcctccc acttgtcgac tgctgttcga ttatcttgag ctaagccata agctaaccca
     4921 gtcaaaaaaa ataccaggta ggcgatcaaa aacatcaccc ctgcaactaa cgcataacgt
     4981 aactttgaat gcataatttc atttaatgct aaaaacattt aactcctcct cctgaaataa
     5041 tcattttata caacaatttt atcacttgat aaatt
//
LOCUS       DAHEYX010000181         5065 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHEYX010000181 DAHEYX010000000
VERSION     DAHEYX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.182"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(93..1997)
                     /locus_tag="MB219_002272"
     CDS             complement(93..1997)
                     /locus_tag="MB219_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCC8427974.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTDMAPESALVQTEDGEFEEVDVDDVDEGDVLLVKTGAKVPVDGTVL
                     TGEGHINEASITGESVPVTKTEGSEVFAGTILENGTIQIWADKVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPTVLVLAFIVWAMTQNVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVINDFSKVDTIVFDKTGTLTVGNPEVAETEVYGDNVE
                     KAYSYLASVETESDHPLAKAVLQHTGTVELFPVEDTDVVKGGGVVAQVDGHRIAVGNV
                     ALMEKENVQLSKKAQKDVKQFEKNGNSLVLTAVDGELKILMGIRDQIRPGVKQDLQEL
                     KNLGVKDLVVLSGDNQGTVDRVARELNLTEAHGHMLPEDKAAYIEKLQQKGHTVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIY"
     gene            complement(2195..2428)
                     /locus_tag="MB219_002273"
     CDS             complement(2195..2428)
                     /locus_tag="MB219_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCC8427975.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            2667..3707
                     /locus_tag="MB219_002274"
     CDS             2667..3707
                     /locus_tag="MB219_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCC8427976.1"
                     /translation="METERQIRELEKAMNTIDNRRLYERFLAVKLVLEGHTRKETGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKAGSKKQQVFLE
                     ETFPSLKKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIG
                     TLDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLE
                     ENARRLALIFLPPYRPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNER
                     PLEVIDRLCVRM"
     gene            complement(4022..4621)
                     /locus_tag="MB219_002275"
     CDS             complement(4022..4621)
                     /locus_tag="MB219_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8427977.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFLEQ
                     LQVQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTVVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
BASE COUNT         1386 a         1153 c          955 g         1571 t
ORIGIN      
        1 aaaaaagcgg aaaatcccag atttaaaaat ctgagtttcc gcttttgtca tttcaaggat
       61 ggactattgt ctcagcctct tctaatttat tcttaatata tttttacttt ctctgcaggt
      121 gctgatactt ctttttgttc tttccgacct ttcaacttat acttcattaa tctcatgcca
      181 ttgaaaatta ccaccaagat actaccttca tgaaccaaca ttccgataga catattcatc
      241 cattcgctga agaagacgct ggtcaacaag accaacacta ctccaactga aatcacgata
      301 ttttgtttca tattatttgc ggtggctttt actaatccta atgcgtgggg caagttgctg
      361 aagttcgagt tcattaaaac gacatcggat gtttcaatcg ctacgtccgt tccgcttccc
      421 attgcaatcc cgatatctgc tagcgccaag gacgggctgt cattgacacc gtccccgaca
      481 aaggcgaccg tgtgcccttt ttgctggagt ttttcaatat aagccgcttt gtcttccggc
      541 agcatatgcc cgtgcgcctc ggtgaggttc agttcacgcg caacccggtc cactgtgcct
      601 tggttgtcgc cggaaagaac cactaaatct ttcacgccta aattcttcaa ttcctgcaag
      661 tcttgtttca ctcctggacg gatttggtcg cggattccca tcagaatctt caattccccg
      721 tcgactgctg tcaatactaa ggagttccca tttttctcga attgtttgac gtctttctgc
      781 gcttttttac tcaactgcac attttctttt tccatcaacg ccacatttcc aaccgcgatg
      841 cggtgtccgt ccacttgagc gacaacgccg ccgcctttga cgacgtctgt gtcttctacc
      901 gggaacagtt ctaccgtgcc ggtatgctgg aggacggcct tggctagtgg atggtctgat
      961 tcagtctcca cgctggctaa ataactgtag gctttttcta cattgtctcc gtacacttct
     1021 gtttccgcga cttcaggatt cccgacagtc agcgttcctg ttttgtcgaa cacaatcgta
     1081 tcaactttgc tgaagtcatt gatgacttcg cttcctttca aaagaactcc attgcgtgca
     1141 ccgtttccga ttccggctac gttggagacc ggaaccccaa tgaccaatgc acctggacag
     1201 cctaggacta agatcgtaat cgccaattca acgttttggg tcattgccca gacaatgaat
     1261 gccagaacca aaacagttgg ggtataccat ttagaaaagc ggtcaataaa gcgctctgct
     1321 tctgatttag aatcttgcgc ttcttccaca agttcaatga ttttaccgaa tgtggtgtct
     1381 tctccgactt tgtctgccca aatctgaatg gtgccatttt ccaatatcgt accggcaaag
     1441 acttcactgc cttctgtttt tgtgacagga acggattctc ctgtgatact ggcttcgttg
     1501 atatgccctt cacccgtaag gactgtacca tctactggaa ctttcgcacc agttttgaca
     1561 agcagaacat ctccttcatc tacatcatct acatccactt cttcaaattc tccgtcttct
     1621 gtttggacca aggcactttc tggtgccatg tcggttaatt ctttgattgc tgaacgtgtc
     1681 tggttcaatg tacgctgttc caaataatgt ccgaacaaga acaagaacgt aacgatagcc
     1741 gattcttcat aattttgaat aaaaagcgca ccaatcgctg caatactgac taagacatca
     1801 atactgatga ctttgacttt cattgcttga aacgcttgaa tggcaattgg cgtaatccca
     1861 aagacagagg cgatgatcaa agaccactct gaaagtccta cgttttctaa aacaaagtga
     1921 ctgaagaaac cgagtacgat taacaatcca ctgatgacgg tgataacatt tttcttgctt
     1981 aatatatatt gttgcatgct ttttccctcc aaatttaatt tctcttttat tgataaacac
     2041 agtataagat aattgcctaa gtaattaatt gactgacatc aagtttagaa agttctcttt
     2101 tatcgtttct aagcacctcg aatcggattg caacaaaaaa agcaatcctg gaaaaatagc
     2161 ttttcttcca gaattgccta gttcatttaa tctgttaagc ggccttgact tttgctttaa
     2221 ggaccggata gcctacgttt tcgatagctt tttgaatctc ttcaatcgaa gtgacagctg
     2281 aatcaaaatt ggctttgact ttgctggagt tgaataatac tttaatgctg tctttatcaa
     2341 caccgttaac tgattttacg gcaccttcaa ttttttgcat acaacttgga caagacaacg
     2401 tttctaattg caatatagct ttttccataa ttcctatctc ctttatgtgt ttttatttta
     2461 tcggcagcag attaatgaac actgcctttt cctttttaca actgtagtat agctcacttt
     2521 tagcaaacga aaattgacac atattaagtt ttgcacttct ttttatcttt ttgctcaatt
     2581 caccatctta tacaaattga gttaaattgt attcgttttt cctcctatag gcgtatacta
     2641 aagaaaacac ttgaggagtg gggaacatgg aaacagaacg ccaaatccgt gaattggaaa
     2701 aagcgatgaa tacgatagac aaccgccggt tgtacgagcg tttcctggca gtcaagttgg
     2761 ttcttgaagg ccacacccgg aaagaaaccg ggcacaccat cggccgcgac gagcatactg
     2821 tcagccacta tgtgaaaaac tactgccata atggaatcca aggtttagtc tccaaaaaac
     2881 aaagcggacg accttcacgg ctgacagatg aacaggaagc ggaactggtc caaatcgtcg
     2941 cctaccacac tccggaggaa gtaggtttca aatcccgtgc caattggaca ttggcgattg
     3001 tgtgtgacct gattctccgg gaatggagtt atggatatac gcaaaaaggc gtggcggacc
     3061 tccttcaccg gctaggacta agctggaccc gtccgacgta taccctcaaa aaagcgggtt
     3121 ccaagaaaca acaggtattt ttggaagaaa ccttcccgag cctaaaaaaa aaattgctga
     3181 atagcgatat tgaccacctc ctattcgagg acgaaaccat gatccgggat taccaagcca
     3241 ttggctgcac ctggtttctc cgcggccacc agcgaaaaat ccccacttat ggacaacaca
     3301 aaggggtaaa attgataggc accttggatt atgaaaccgg tcggatcatt tgtacagaag
     3361 aaggggcata cgacgcagcg gccttcctcc gttttctgga gcggacgttg cgggaatacc
     3421 ccaccggaaa aatcgtgatg gtattggata atgcccttat ccaccacgcc aagctgatcc
     3481 agtcttttct tgaagaaaac gctaggcgcc tggcactcat cttcctccct ccctacagac
     3541 ctcagttgaa cctaatggaa ggcgtttgga agtggctcaa ggaatcggtc atcaacaatg
     3601 ttttttttga ccacgtccag aaaatcaaac agtcggtccg cggtttctta gccgatgtaa
     3661 acgagcgtcc ccttgaggtg attgaccgac tgtgcgtgcg gatgtaaagt atttaattca
     3721 acctatatag aaagaagtct tgtacttcat tttagtctaa tgacagtcat aacatcatcg
     3781 acagccccgt tattgagtca attctattca ataatttctt ctacttgtaa taacgttgta
     3841 ttcaacgctt aaaatattcc agcattaaac accagttcca cactgttctt tttcaataga
     3901 tacaccgatt ctttgcgaga taattggtat ttatcaggag atttaggacg gttttctacg
     3961 ttcattttcc cacctcttga atttttcctt ctattatata ggtatatagg gcgttattct
     4021 gtcatagatc caaactcttt actgcggtat ctttgtcttc ctgggtgatc ccaatatagc
     4081 gtaaggtttc ccgttgtgaa gaatggttta gtagtgactg aatgatctct gttttggttc
     4141 cttgcttgta caaaaaatag ccaaaggtct tacgcatcga atggggacca atttctttca
     4201 acccaaccac cgttcctgct tcatgaatga tccgccaaag gctttgtcgg gtcaaaggcg
     4261 tatctgctcc ttttcgactt ggaaacaaat acttgctgaa gtcttgtact tgcagctgct
     4321 caagaaattc tgattgaaca taatcaagaa tcacttttcg taatttgttc gaaatgaaaa
     4381 ccgaattgtg tttagctgtt ttttgttcct tcatggccag acgttctttg acgatcaccc
     4441 ggccacggga aatttctaac acgtcactta atttcaactg tcttaaatcg gagattcgat
     4501 acgccgtatt gatcccgatc gaaaaaagca ccaaatttcg ttgcccatat tttccggaag
     4561 ccaaaattgc cttcatggcg tcgatctttt ttttatcgcg aatcggttcc acgctcttca
     4621 tttttagtcc cctctcaaat gttacttaat atgattataa cagataataa gtcacattta
     4681 aacaagctat agaaaaaagg ccctaaaaga aaagtgattt cctcttagag cctctaagga
     4741 tggagcgatt ttgatgaatg tgacagaatc cgattgtgtc acattaggct tttgatattt
     4801 tcggtaagat ttgattatct cagaacatta atattgcccc tttaaaaaat tttgtcagta
     4861 cttttaagtt attctattct gaaaattcaa aattttcagt ctatataatt aacttatatt
     4921 cagttttacc aaatgataag ctttaaaaag tattcagaat ttagtcatgt tatggatcca
     4981 ataaagtaga tatggaatat ttattttctc ttgtctaatt tataagaata tgatttgtca
     5041 aagaattaaa gctatgaatt attca
//
LOCUS       DAHEYX010000182         4990 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHEYX010000182 DAHEYX010000000
VERSION     DAHEYX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.183"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            44..1185
                     /locus_tag="MB219_002276"
                     /pseudo
     CDS             44..1185
                     /locus_tag="MB219_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1311..2390)
                     /locus_tag="MB219_002277"
     CDS             complement(1311..2390)
                     /locus_tag="MB219_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCC8427978.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2493..2933)
                     /locus_tag="MB219_002278"
     CDS             complement(2493..2933)
                     /locus_tag="MB219_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCC8427979.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2990..3400)
                     /locus_tag="MB219_002279"
     CDS             complement(2990..3400)
                     /locus_tag="MB219_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCC8427980.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3580..4014)
                     /locus_tag="MB219_002280"
     CDS             complement(3580..4014)
                     /locus_tag="MB219_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCC8427981.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4114..4962)
                     /locus_tag="MB219_002281"
     CDS             complement(4114..4962)
                     /locus_tag="MB219_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCC8427982.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1817 a          961 c          842 g         1370 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     1321 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     1381 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     1441 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     1501 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     1561 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     1621 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     1681 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     1741 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     1801 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     1861 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     1921 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     1981 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2041 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2101 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2161 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2221 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     2281 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     2341 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     2401 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     2461 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     2521 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     2581 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     2641 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     2701 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     2761 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     2821 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     2881 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     2941 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     3001 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     3061 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     3121 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     3181 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     3241 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     3301 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     3361 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta
     3421 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     3481 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     3541 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     3601 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     3661 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     3721 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     3781 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     3841 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     3901 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     3961 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     4021 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     4081 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4141 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     4201 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     4261 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     4321 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     4381 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     4441 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     4501 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     4561 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     4621 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     4681 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     4741 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     4801 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     4861 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     4921 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     4981 cattaataat
//
LOCUS       DAHEYX010000183         4938 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHEYX010000183 DAHEYX010000000
VERSION     DAHEYX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.184"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            37..723
                     /locus_tag="MB219_002282"
     CDS             37..723
                     /locus_tag="MB219_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCC8427983.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            725..1669
                     /locus_tag="MB219_002283"
     CDS             725..1669
                     /locus_tag="MB219_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCC8427984.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1868..2512
                     /gene="pcp"
                     /locus_tag="MB219_002284"
     CDS             1868..2512
                     /gene="pcp"
                     /locus_tag="MB219_002284"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCC8427985.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2591..3868
                     /locus_tag="MB219_002285"
     CDS             2591..3868
                     /locus_tag="MB219_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427986.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4052..4801)
                     /locus_tag="MB219_002286"
     CDS             complement(4052..4801)
                     /locus_tag="MB219_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCC8427987.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1590 a          868 c         1057 g         1423 t
ORIGIN      
        1 attaaaaaaa tattgtcatt ttatggagga aaatatatgg aaaattatct tagcctttta
       61 ggcgtcttga ttgttattct cgggtttgca tttaaattag actcgatatt gatcgtaatg
      121 gttgctttag ttgttacagc tttgactggc ggtcttggca ttcaaggaat gttagaactt
      181 ttaggtacaa gttttgtaaa caatcgcggg atggcgattt ttatcatcat tatgttggca
      241 acaggaacgc ttgaaagaaa tggtctgaaa gaatcagcgg cttccttgat caagcgattc
      301 aagaaagtat ctgctggtat gattgtagat atttatggga tctttcgaat gatctttgcg
      361 gcttttaacg tgagttttgg tggagttgct ggatttgttc gtccaatcat tttaccgatg
      421 gcattgggga ccatcgaatc aaaagggtta gaagtccatc ctaagcatga agaagaacta
      481 aaaggtatgg cttcagctat ggaaaatgtc tgctggttct ttggccaagt actattcatc
      541 ggaggagcag gggcgttgct agtccaatca acactgaaag atctaggata tgacgtaaca
      601 ttgggacagc ttgctttagt ggaagttcca gtggctatca cggctttagt tgtggcaagt
      661 gtctacttct atcttaaaga aaagaaatta gcgaagaaat attatccagg aaaggggcaa
      721 taagatggac ttttttacaa atcctgatat tttattaggc gataaattac tggagatcct
      781 ttatatcgtg atgggactcg tattgattta cactggagta agaaatttaa cagataaaac
      841 caatcctcat cgttatgggt cagccttttt ctggacggtc ttaggggtag ttattgcagg
      901 aggaagatgg cttccggcaa tcgtgaacgg tggcttgatc tttgcgatga ccataccagc
      961 aattgctaag aaagtcagca aaggagaaag ccggcttcca tcaaaagaat atatggaaaa
     1021 aatgtcagac aaactaggaa tgaaaatttt tattccagct ttgagtatcg gtgtctttgc
     1081 gatcttgttt gctctattta ccaatttagg tgctttagtc ggcgtgggcg tcggtgtttt
     1141 tgcagccatg ctaatcttga tgttcttttc tcgagataat cgaccaagta catttttaga
     1201 tgatgcagca gatatgctgg gcacagtagg gccgttaagc atgttgccaa tgcttttagc
     1261 ttctctaggt gcggtattta caagcgctgg agttgggaca gtgatttcca atgcagtagg
     1321 tacgatcgtt ccagaaggaa atgtgaacat cggcatcatc atttacgctc ttggcatggt
     1381 gatatttacg gtaatcatgg ggaatgcatt tgcggccatc accgtgatga cggttgggat
     1441 cggtgcacca ttcgtattta gtcatggagc gaatccagcg ttaataggaa tggttgcatt
     1501 gacttgcggt ttttgtggga ccctgctgac accgatggct gctaacttca atatcgtgcc
     1561 tgtagcgatg ctggagatga aagacaaata cggagtgatc aaaaaccagt tgtttattgc
     1621 gttatttatg ctagttttcc aaattgctta tatgattcta tttaaataat agaagagagg
     1681 ggttgtgaca gaataattgt cgcaacctct tctaacattg tagtggaaca ctattttttt
     1741 ataagaatat attaagtttg atctgttatg ttatttaaac tattgtttcc tttatttacg
     1801 ctgttcaagg aaaatggtat gataaaagat aaccatatgt taaatcaatc ggacggagga
     1861 acaacagatg aaagtactag taacaggttt tgatccattt ggtggggaca aagtgaatcc
     1921 agcctatgaa gcagtaaaaa aaatgccaga tgaaatcagc ggtgctgaaa tcatcaaagt
     1981 ggaagtaccg accgtattcg aaaaaagcag tcaagtagtc aaagaagcca tccaacagca
     2041 ccaaccagat gtcgtgatct gtgtcggaca agcaggtggg cggtcagcag tctcgttcga
     2101 acgtgtcgca atcaatctag cagaagcaag aatccctgat aatgaaggaa atcagccatt
     2161 cgatacggct ttagaagaaa acggtccagc cgcttacttt acctcattgc caatcaaagc
     2221 aatgacaaaa aatgtacaag atcatggtct accagcgtac atctcttata cagcaggaac
     2281 atttgtctgc aatgatatca tgtatcgttt gcttcatttg atcgaaacac aattcccggc
     2341 aattcgaggt gggttcatcc acgtgccatt ctcacccgat caagtgatcg atcgtccagt
     2401 gggtacgcct tctatgtctt tagaagacat tgcttcttct ttgacttatg cagttgaagc
     2461 agcagtagaa aataaagaag atatttccgg aaatgccgga acaactcact aatttagcaa
     2521 gaaacactgg ctggttgcta acgttatgtt agtagccagc tttttgtaat tagaaaggaa
     2581 agaaacagcc gtggaagaat ggcaatccgt ctttgaagaa tggtttccaa aagaaatcag
     2641 caaaagctat cccatcaaaa tttctaaaca atacacaagc agtcaacgtt gggagatcta
     2701 tgcaaaatta acgaaaaaac agcgggaact agtcgataag caccgccgat atctaatcag
     2761 ctctcgtttt atggaagaac attatttagc tgcaacagat tgggtgtttt ctgacttcaa
     2821 gatcaatccg ttttttcgaa caaaacgaag ccagcaaaaa ttgtattgtg agtgtggtag
     2881 agaattaaaa gtgcaataca tcgttaaatc gccaaaaacc ggaaagatcc taaaactagg
     2941 catcaaccac tttgccgatc acctgcatgt ctctccgaca gtggcagctt cgatccatca
     3001 gggaatgaca aaagttgatc tggccttaga cgaattatta tggcttaagc agaagaatat
     3061 cgattttcct gaaggactat ggcaaaaata ctgttttgtc ttgtaccaaa atcgtcggat
     3121 gaaacagcca tatttaccgg atataaaatt agctcagcgg ctcgcagaat ttcgccaagt
     3181 agaaatgccg atttacatag cggattatca agcattggaa aatgaaatca agaaaataag
     3241 tgaacacatc aatgggcagt caaaaaagcg acagataaaa aaagaactat ttgacgactt
     3301 tgcagaagaa ttagtaaagg atgtagaaga attcttgatc aactatcgcg catttttacg
     3361 taaagattgg caatccatcg tatatgaaga agtgccagta catcctaatg cttattttga
     3421 aacatttatt tccgtgttga gaaagacaaa acgtcaacga acaccagaag tcactgctca
     3481 gatggaatat tttgcgaaaa accaacgatt catccaacca aaaatctatc tatttatttg
     3541 gaagcaatat tgtcgctatg gttttacaga agggttcttc gacagcattc ctcggatagt
     3601 cagaaatggt tttttaaaag tattgcgtaa agaacgagaa gctatacaat ctgctgataa
     3661 gaaagaccgt acagtctcaa aagaaaaatg gcaactagta gtgaaagata tccaatctgg
     3721 aaatgtgcaa gagacaatcg acaaatggaa aggaaaacat tatcgtttta ctgaagctca
     3781 gaaacaagcg ttggaatatt atcagaagtt agaagaatca ctgcgtttca atgatgaggc
     3841 aagaaagtat ctgaaggaat tactataaaa aatagaggct gggacagaag cgtttaactc
     3901 caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat ttcagcttat
     3961 ttccgaagga gttgcttctg attccgccgt ttatacgtgt ttagagcgtg agacaaaagt
     4021 gttctttact tttgtcccac gctcagtttt tttatgcgtg ttgttgtgcg ttgtagtatt
     4081 cttcttccat tttttgcata cgttcacgtt ctttttggtt ataccaaggt gaaacggtaa
     4141 atgcaaggac atcattcagc aagtaaacag agctgttcac gaacatcgcc aaacttgcag
     4201 atccatgcgt aaatgaaata taccaaagga ccatttgagc aagccctgaa gctaaccacc
     4261 agtaatattg gttgttgtaa cgcaagaaac tgatgatacc agcagtcaag ctaatagaaa
     4321 aagcaatggc atctacccac ggccgaggat catctgtata acgaccaatc aggaatcctg
     4381 aaacaagata gacaaggaaa gtactgatga ttgcgataaa ccagcttttt ccagtgaatt
     4441 ttcggatttt tgaagccata ttatgattcc attctttact taacaatacg gggatatcca
     4501 atgtgatcat ataagcaatc tgttcaccga tacttaaata gttctttgct gaaaacccaa
     4561 cgataataaa acaaatcgca gacaaaatac ctaaaatacc gttgactgat tttgccgcat
     4621 tgatggataa aatacacaat acaccaagcg tggtaccgat aaatgtaata acggacagcc
     4681 aagtgatggg actattgaca agagtcatga cctggcagcc aagactgaaa aagaaaagat
     4741 aataattttg ttgcggccag ccttttagct gatgagctaa aaactgaaaa tatgacttca
     4801 tgtatgagga ctccttaaga cactaaatgt agtgtgttct gtatttacta acacaatcta
     4861 ttgtatgctt ttcttgaaaa aaaactagtc tttcaattat aaataatttg tgttatgata
     4921 attttctgtt tgttttct
//
LOCUS       DAHEYX010000184         4880 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHEYX010000184 DAHEYX010000000
VERSION     DAHEYX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.185"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            23..841
                     /gene="purR"
                     /locus_tag="MB219_002287"
     CDS             23..841
                     /gene="purR"
                     /locus_tag="MB219_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCC8427988.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1344..2717
                     /gene="glmU"
                     /locus_tag="MB219_002288"
     CDS             1344..2717
                     /gene="glmU"
                     /locus_tag="MB219_002288"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCC8427989.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2811..3782
                     /locus_tag="MB219_002289"
     CDS             2811..3782
                     /locus_tag="MB219_002289"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCC8427990.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3788..3890
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3956..4525
                     /gene="thiT"
                     /locus_tag="MB219_002290"
     CDS             3956..4525
                     /gene="thiT"
                     /locus_tag="MB219_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCC8427991.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4606..>4880)
                     /locus_tag="MB219_002291"
     CDS             complement(4606..>4880)
                     /locus_tag="MB219_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8427992.1"
                     /translation="KKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIY
                     VIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1582 a          864 c         1072 g         1362 t
ORIGIN      
        1 ttcttaaaag gagttggaat aggtgaaagt acgtcgcagt gaacgtctag ttgacatgac
       61 acagtattta ttgaatcacc cgcatgaatt gatttcctta acaagctttg ccgaacgtta
      121 tgaatctgct aaatcttcta tcagtgaaga tttgggaatc atcaagaaaa catttaaaga
      181 acgagggtat ggaaccttgg aaacaatccc gggtgctgca ggtggtgtat tgttcatccc
      241 tgaaatttca tatgaagagg cgaaaaaata tattgaatct ctagcagaac gtctatcaga
      301 acaagaccgt ctgcttccag gaggatatgt ttatctctct gatttgttag gagaacctga
      361 actgctgcgt caagttggaa gaatcattgc ttctaaatac cttggtgaaa aaatcgatgc
      421 tgtaatgact gtagccacaa aaggcgtacc tattgcccaa gctgtgtcct attatctgaa
      481 tgcgccattc gttattgtac gccgggactc aaaaataaca gaaggatcaa cagtaagtgt
      541 caactatgtg tctggatctt ctgaaagaat tgaaaagatg gaactgtcta agagaagttt
      601 gaaacgtggt tcacgtgtcc ttgtagtgga tgatttcatg aaaggcggcg gaacagtcaa
      661 cggaatgaag agcttgattg aagaattcga atcagaactt gttggcatca ctgtatttgc
      721 cgaatcaaaa ttcaacggtc atcgcgccat cgatgattat acgtcactgc tttatgtcaa
      781 agatgtagat actcaaacga aaaatattac ggttgtgcca ggaaattatt tttcagaata
      841 agaggttgtg aaaaagctgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
      901 tccacatttt ttcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
      961 ctagaggttg tgaaatcagc gtcctgtcct gagtattagc tcaataaacg gaaaaattgc
     1021 tcctcatatt ttgccgttta ttgagctaat accgaaggtc agctgattga aaaacgttta
     1081 atgaagaggt tgtaagccta ccgtttaact ctaagtcaca agaaacgatt tcaaaaaaca
     1141 gcttttcatg ttttatgagt agtgtgactt gtgaccgagg agttggcagg agagcaccgg
     1201 ttaccaaata gataggcccg ttatggtcta tctatttttt tatccagaaa acgctgggat
     1261 aaaagaaaat cacttgtttt gttgaaaaaa tgggtatact gggataaact gaaatcagca
     1321 aactatattt aggagtgacg cagttggacg cacgttatgc aattattttg gcggcaggga
     1381 aaggtacccg catgaagtca aaattatata aagttttaca tcccgtttct ggtaaaccta
     1441 tggtagaaca tatcattaat cgggtcagtg aaacgaaacc agatgaagtg attacgatcg
     1501 ttggacacgg agcagaacaa gttaaagcac aactaggaga acgcagtaaa tatgcgttac
     1561 aagcagaaca attgggaaca ggacatgctg ttttgcaagc tgcttctttc ttggaaggaa
     1621 aaaaaggaac aacgttagtt atcagtggcg atacaccatt attgactact gaaacgctga
     1681 acaatctttt tgaatatcac caaggaaaaa atgcaagtgc aacgatcttg acggcacaag
     1741 cagaaaaccc aacaggatat ggacgaatca tccgtgacca tattgggatc gtagaaaaaa
     1801 tcgtggaaca aaaagatgca acaccagaag aagctcttgt acaagaaatc aatacaggaa
     1861 cttactgttt tgacaacgaa gctttgttcg atgcgttaag caaagttgga acaaacaatg
     1921 ctcaaggtga atactatcta acagatatca tcgaaatctt aaaagaagaa ggccatacag
     1981 ttgctgctta tcaaacggat gattttgaag aatcaatggg agtcaatgat cggatcgcgt
     2041 tagctaaagc aaatgagatc atgagaaagc gaattaacca gatgcatatg gtcaatggcg
     2101 taagctttgt cgattcagca acgacataca tcgatgcagg cgtagaaatc ggtccagaca
     2161 cattgatcga agcaggtgta caaatccaag gaaatacagt cattggttct gattgtgtca
     2221 tcgggtcaca ttcaaaaatc gtagacagta ggattgagga tcacgtcgtc attgaaaact
     2281 cagtcattga aagcagccat gtcaagaaac atgcagatgt gggaccttat gcacatttac
     2341 gaccaaaagc tgaaattggc gaaaacgtcc atatcggaaa ctttgtggaa gtgaaaaatg
     2401 ctcaaatcgg caaaggaact aaagtcggtc acttgactta tgttggtgat gctacgctgg
     2461 gagaagaaat caatgtcgga tgtggtgttg tcttcgtcaa ctatgatgga aagaacaaac
     2521 atcatacgac tgtcggcgac cacagcttta ttggttctag cacgaatatc atcggacctg
     2581 tcgaggtagc taaaaacagt tcgattgctg ccgggtcaac aattacagac aatattccag
     2641 aatatgcgct ggcaattgcg agagcaagac aagtcaataa agaaggatat gcaaaaaaac
     2701 tcccttatct aaactaagac ttttgcaaaa tcacttgatt tatttaaaaa atatgactac
     2761 tatttattag gtagaattct agagttgtga aaaagaaagt ggaggtcccc atgtcaaaac
     2821 attactttga cccaagatta aaaatttttg cgttaaattc caatcgccca ttagcagaga
     2881 aaattgcagc agcagtcggc gtcgagttag gaaaatcatc tgttactcaa tttagtgatg
     2941 gagaaattca agtaaacatt gaagaaagta ttcgtggatc gcatgtctat gttattcaat
     3001 caacgagcag cccagtcaat gataacttga tggagttgct gatcatgatt gacgcactga
     3061 aacgagcaag tgcaaaaaca atcaacgtag tcatgcctta ttatggctat gcgcgtcaag
     3121 accgtaaagc acgtgcacgg gaaccaatca cagcgaaatt agtagcaaac atgattcaaa
     3181 aagctggtgc aacaagaatg ctgactttgg accttcatgc agttcaaatc caaggtttct
     3241 ttgatattcc agtcgatcat ttgatgggtg cgccacttat tgccaattat ttcttagaaa
     3301 aaggaatcaa aggcgatgat gttgtcgttg tttcaccaga ccatggtggt gtcacacggg
     3361 cacgtaaatt agcggaattt ttgaaatctc cgattgcgat catagacaaa cgccgtccaa
     3421 aagcaaatgt tgcagaagta atgaatatca ttggacaagt caaaggcaaa acttgtgtac
     3481 tgatcgatga tatgatcgat acagcaggta cgatcacatt ggctgccaat gctttgaaag
     3541 aagctggtgc aactagtgtg tatgcttcat gtacacatcc agtattatcc ggccctgctt
     3601 tacaaagaat agaagattca gctatcgagc gtctagttgt tacagactcg atctatcttc
     3661 cagaagatcg taaaatcgac aaaatcgatg aagtaagtgt tggcggacta atgggagatg
     3721 caatcaaacg tatccatgaa aacaaaccag ttagtccatt gtttgaaaca aaaaataaat
     3781 aataaataaa ctcacaaagg ggagtccaat tgggctgaga ttgaatctat ttctaaaccc
     3841 ttcgtacctg tatcggttat gcgagcgtag gaattgtgaa taaacagctg tttgactatt
     3901 ttcgaaagct gcttcctcct ttgtgacttt tttattcaaa ggaggaagtt tttttatggg
     3961 aaaaaataga gtttgggtag aaggaacgat tgttgcagcg ttagcgatgg tgttgtcgtt
     4021 gattccgatc cagatcggca gcagtttttc tatttcatta gggcagattc cattgactct
     4081 ttttgcgtta agaagaggga caaaggcagg gatgctggca ggtttcattt ggggcatcct
     4141 acattttccg ttgggacaag tttattattt aagtgtcgtt caagtgttga ttgagtaccc
     4201 gattgcctat acttttgcag gcgcagcagg gctcatggct tcaaatgtcc aaaaaggcta
     4261 tgtagacgat aatacgaaac aaattagaaa aagcatcgtg tttggtgcat tgattggcgc
     4321 actttccaga tatttttggc attttattgc aggagtggtt ttctggggag cgtatgcact
     4381 atgggggatg aatccttggc tcttttcttt cgtaatgaat ggtgccagcg ggttgagtac
     4441 agctattgta acgatcatcg ttttattagg gatccaacaa acgactcctt cgatttttgt
     4501 gccagataaa cgacaggagc tgtaaataaa aaagaagcaa gaaacttgag acagaatcat
     4561 gtcctagttt cttgtttcct ttttattttt tgataagaaa tagatctact tcaatggcag
     4621 ataagaaaga tcggcatctt ctaccaattg ccagtgtcga tcggtgacag agtagattaa
     4681 agtagcgatg atttcctctt ctcgataccg ggtgcctttt aatttccaaa tataatcttc
     4741 aatcacatag attgggatac gatatttacc atcgatcaaa ccaacttgat caaaagaaaa
     4801 aatgatccct tcctgcatca aattacgcat ttctttcaat aattccacac tgattttttc
     4861 aacaggtgtt tcttttttga
//
LOCUS       DAHEYX010000185         4869 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHEYX010000185 DAHEYX010000000
VERSION     DAHEYX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.186"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            144..944
                     /locus_tag="MB219_002292"
     CDS             144..944
                     /locus_tag="MB219_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCC8427993.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            963..2831
                     /locus_tag="MB219_002293"
     CDS             963..2831
                     /locus_tag="MB219_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCC8427994.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2824..3315
                     /locus_tag="MB219_002294"
     CDS             2824..3315
                     /locus_tag="MB219_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCC8427995.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3303..3857
                     /gene="srlA"
                     /locus_tag="MB219_002295"
     CDS             3303..3857
                     /gene="srlA"
                     /locus_tag="MB219_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCC8427996.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3875..>4869
                     /locus_tag="MB219_002296"
     CDS             3875..>4869
                     /locus_tag="MB219_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCC8427997.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1693 a          762 c         1027 g         1387 t
ORIGIN      
        1 gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac attttttagc
       61 tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg gatttgtaag
      121 aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga aaagtagtaa
      181 ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga
      241 atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa aacctactat
      301 ctttaaaagt cgatgtgtct tctaaaacgg acatagaaga agggatatat aaagtaatga
      361 aacatttcaa gacgatcgat ggtctagtca ataatgcagg aataaatatt ccttctcttc
      421 taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt tttgataaga
      481 tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga agaatcttgg
      541 tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg gaaggatcag
      601 aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact cgttcttggg
      661 caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg atactggaag
      721 aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga ggtataagcg
      781 tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga aggagtggta
      841 agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca agttatatca
      901 ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata ggggggacgc
      961 acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact cacaagaaag
     1021 tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata aaaaaagata
     1081 ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa aacaaacatt
     1141 ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga aaattgaaaa
     1201 cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat aggctgatcg
     1261 aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc agcagaggaa
     1321 cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat gtaaagatta
     1381 caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta cgactacttt
     1441 tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt ctagaagaaa
     1501 aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa atcacccttt
     1561 taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg ttgaagaagc
     1621 ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa ctgatcaatc
     1681 agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt atcagttttc
     1741 ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct gatttgcgct
     1801 tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat ctggatgagt
     1861 ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg gtaatttttc
     1921 gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct ttttcttacc
     1981 aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag atctctaaag
     2041 tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa aaagagcatt
     2101 tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca ttaatgatcc
     2161 aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt tcagaagaac
     2221 aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa
     2281 atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac tcttggttaa
     2341 gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct actgtttttt
     2401 tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc
     2461 ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg aaaagagaat
     2521 atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc aaccagctat
     2581 ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca gaaacaggag
     2641 gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa gttgagaatg
     2701 aattatttga gctatacgat atcctatttg aactcataaa aatgggaata gatgagttta
     2761 ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa ggagagagac
     2821 ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt gctgcaagca
     2881 gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact gcgaagaaaa
     2941 ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat tgttatgcta
     3001 gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt tacatctttg
     3061 gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat cgatcgttac
     3121 catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa tgccagagaa
     3181 gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt gccttttatt
     3241 gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag gagtgacaaa
     3301 cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa acaggtgcag
     3361 aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg atggttgcaa
     3421 tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc aaattatcag
     3481 cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt atgttgggaa
     3541 atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca agctattatg
     3601 ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt aatcctggag
     3661 aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta aattcgatgg
     3721 agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt ggttggataa
     3781 ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc aaaaaagtag
     3841 agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta agatcgtaag
     3901 aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata aacacaagtt
     3961 catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag aagaactgac
     4021 aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc
     4081 aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag ggataccaac
     4141 catcaatatc gtttccacag gaaaaagtgg cccattagca caatatatca ctgaagatat
     4201 ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg atgataacaa
     4261 agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt ctaaaaagat
     4321 aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa
     4381 agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga tccggacagt
     4441 gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt ctgggatcgg
     4501 taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat taacaatcat
     4561 tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg cagttattag
     4621 ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc ctccccaatt
     4681 agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg
     4741 tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat cagttcttta
     4801 ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa gttttggatt
     4861 atatagctg
//
LOCUS       DAHEYX010000186         4861 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHEYX010000186 DAHEYX010000000
VERSION     DAHEYX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.187"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1219)
                     /locus_tag="MB219_002297"
     CDS             complement(<1..1219)
                     /locus_tag="MB219_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCC8427998.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1280..1855
                     /locus_tag="MB219_002298"
                     /pseudo
     CDS             1280..1855
                     /locus_tag="MB219_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2036..3229)
                     /locus_tag="MB219_002299"
     CDS             complement(2036..3229)
                     /locus_tag="MB219_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCC8427999.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3313..3513)
                     /locus_tag="MB219_002300"
     CDS             complement(3313..3513)
                     /locus_tag="MB219_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCC8428000.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3937..4170)
                     /locus_tag="MB219_002301"
     CDS             complement(3937..4170)
                     /locus_tag="MB219_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428001.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4177..4608)
                     /locus_tag="MB219_002302"
     CDS             complement(4177..4608)
                     /locus_tag="MB219_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCC8428002.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1003 g         1423 t
ORIGIN      
        1 acatttctac aaaaaaatag tgattctgtt ttgaaaattc gatttttagt tctgcatttt
       61 cgtaaacatc caacattttt ttcgcactgt atagacctaa cccgccattt tttttagagg
      121 tttttcctct tttgaccatc tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta
      181 tactaaaacg tgatccctct ttttcttcca gcagattgat cgtaatcgga ctctgatcgg
      241 ctacacgatg atcgaatgcg ttagaaataa aaatagataa acagcgaata aaaacaacca
      301 gatcaatcgg gattgttttc acttcgacag gaataactac ttggatcggg attttctccg
      361 attctgcttt ttctagaaat tttaaaaaga cacccctgac agctggggat acaatatttg
      421 ttagctgttg tatttgcggt ttttcaaaaa tagctgcaga atgatcttct aatatttcta
      481 aaaattccaa cgtttctttt ttattttcct gaagagtaat ttttaatgaa aacagtagac
      541 ttttataatc atgtttgaat tcctctagat gatggtaata ttcttcattt atttgataca
      601 gttgaagcga ttcctgatgt tgtctttctt ttacttggaa actgatcaac ataatggaaa
      661 aaaaaaaaga aaaaaaactg atgactatcg acaaaagaga tagaagcaga taattagatg
      721 agcggaagtg aacacttccc aataccaagt aaatcacgcc ccagcttaat aaaatacaaa
      781 tacctccaaa atagtgttca gttggataag taaaaagcaa gttgagaaat ctcgtctgtt
      841 tgtctatact ttttactaat agatagaaaa taaatgcgat aagtagtgca aaaaaacttg
      901 ctgttccaag atgtctcttt cctaaataat ccatcagatc aaaaaccaga aaaaagctga
      961 gtccagtgat agcaaaaaaa agattactga tcaaacagct gataaacagg tttttcgtag
     1021 taaaataaaa aagaaacatc aataataagc caactaggta aggaaagctc actttaggga
     1081 taggaagtaa aagtgtcacc accatcaaag ctatacaacc aaagctgttt tttactagca
     1141 gttgtttcga aaagaataaa tagataccta aatctaaaat caaagtaaag gtcaaataaa
     1201 gtgtcactgt tgcgaccatc ttattcctcc attgttttgt ttcaaaatgt acacatattt
     1261 ttttaatagg agcctgcata tgaaaaagta ttttatcata gaagataacc agtttcacag
     1321 cgaatggatc atcagagaat tggtcaatca tcatatcgag aatatcgtgc agctcacttc
     1381 tatctctcaa atcaatcagt tgatccagtt gggcactatt gataatcacg atgttttctt
     1441 tatcgatatc gacttgaagc aatcactcac aggtattgat gtcgccgaac aaatacgtaa
     1501 aaaaaatgca catagtaata ttgttttttt caccgcttat acgaattttg cagtagagat
     1561 catcaatcgg aaaatccttc cgattggata tatcagaaaa gacaggaatt tatctgaaca
     1621 aattcgtcaa gtgctagaac agatcaattg gaaagaacag cagatcgtaa atgtcgataa
     1681 aatctttatt gatcaaaaag gtggaagtct cgtcctgtat cctcgggaaa ttttatatat
     1741 tgaaagtatc aaaggtttga aaaaccaagt tatggtcaaa acaactacag aagataaatt
     1801 agtgaatact tctttaaaac aattgaagac agagttagaa aagtatgctt actttttctt
     1861 aaaaattata tgggatttta gattatcttt ttagaagttc ttgaatgtcg gtatagcccg
     1921 tgtttccggg cttttccggc atttttgata ttgttagata cttacaaatg ttcttattac
     1981 ctctcaccat ttccccctca aaagtagtaa atggagtagt aaaaccggcc ttgtgtcagg
     2041 ctgccagccg ttccatttct gcccttgcgg aatcgaatgt ggcgtgtgca taatagttca
     2101 gcgtcatggt gatattggaa tgtcccataa tgtactgcaa ggctttgggg ttcattcccg
     2161 cattggctaa ccgggtgcag aatgtatgac gcatagcatg gggcgtcgtc gttttcggca
     2221 acgcttcttc atggctcttg ttgtacttct tcacaagcct gccaaacatc ccgccataat
     2281 ctaccgccgt catgggatag ccgttctgtt tgagaaacag gaaattggca tacccgtcaa
     2341 taatgaacgg ctttgcacct ttgcggtttt ttatcactcg ctggaacgcc tgatacactt
     2401 cctcattcat ggggattttt ctgacgccgc tttgggtctt gggttcgtca atatagtaac
     2461 cgacgccggt atttctcaaa agctggtggc tcacgatgat tacccggttt tcaaaatcca
     2521 aatctttgtc cgtcagtccg cacagttccg aaatgcgaag ccctgtcccc agcagaataa
     2581 cgattgcgtc ataatgcttt tggtaaacct tatcaccttg aataaaggac agaaaacttt
     2641 cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc gtcgtccaga acgtcactaa
     2701 gctggaagtc aaacgggttt tttcggatgc aatcgtcctg tatggcggtg taaaaagccg
     2761 ctttcaggga acgcttgtcg ttattgatgg tcttataaga aaggcccttt tctttcatcc
     2821 gaagcgccca ttcctttgcg tcggacagct tcacactgtc aatcgggcag ctccccagcc
     2881 gatcttgttc cagaatacgc atcagccgtt cccggcccag tttggtgttg tgcttcacat
     2941 tgccccggtg cctgatctgc ttggcgtaga gctggcagac ggtcattttc ttgccgatgg
     3001 tgtctatccc gtcgtcaagg tctttctgga tttctttctc cttttcccgg agtgaaatat
     3061 cctcccgttt acctgccggg gtcttatccg taggcactaa cttccatgag taaacaaact
     3121 gcggttttcc aaaggtatca acatatttgt aagcatatcg tccgtctttt ctctggctct
     3181 ctccagaccg caaaacgcga tttttgctgt ctcgtctttt ttctgacatc tcgtcatgct
     3241 cctttccatg acggaaagag ccgcatatat gcttataaat tattataaca tatctgcggc
     3301 cctttttcac tattagattg tgtccaaagt gtcaattatt ttctcaaact gcttgcgttt
     3361 gatctggatg cggttgccat tcataatgac ccagccagag gccggatttt cctcggccag
     3421 cttacgcaat ttgttttctc cgatacggaa atatttagac gcttcctcaa tcgtcagcgt
     3481 gtatttttcc caaataggca catcatggtt cataatgcag ccccctttca caaattcaaa
     3541 ggtttgtgtg atttaggggc atagacggac ggctgctggc acagctccac gggactttca
     3601 cccccgcgtg gttctcacgc ggccctaccc attgcctgcg acgcttttaa cgctcggact
     3661 gtggctataa gggagtatca tagtcctgcc tgtacgtcgt cgcccgcaag ccgccacgct
     3721 cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta gggaaaagta tcatggcgca
     3781 ccagccgtcc ggctcggcac agacagaatg tgtccgtatg ccctatgatg cgccgccgtt
     3841 atcttgcggg catatttatc aaggtgcgat caggccaccg ggaaaacacc gaaacgggga
     3901 aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag ttcgtgacga tagcgtggat
     3961 gagcttgatt tccagacggc gcttcatggc ctcgtccaga cacacatgcg gatagccgtt
     4021 ttcgtcatag agcgtccggg tacacagctt gttcatgtaa ccgctgtaat actgcaaaac
     4081 gcgctctacg gctccggcat ccccagcgcg ggcggcctcg atcaccgaaa ggggcaaaag
     4141 ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa cctcccttcg gcatcaacgc
     4201 ctttagctgt ttccgcagct ccgataaggt atttgtcctg tgccgctgaa cggcactccg
     4261 ggacattccc acaaggccgc cgatctcacc atctgccata tccagaacac agtgcaggat
     4321 taaaatactc tgctccatgc tgggcagcgc ggcaaaggct tcggcaacaa gctcattgtt
     4381 gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg tcgctggggt agtgatctac
     4441 tgtacagagc ttgtccaaat ctgcctgtga caaagagctt agtgacactt cacggtcacg
     4501 gcggcgcttc atttcagcca agtagctttt ggcctcgtta cgcagtaccg tcttgcaaaa
     4561 agcatcgaac atgcaccgcg cttcatagtc gcgggggata gcatccatct tctcaccccc
     4621 tttctgtggg ggacttaggt ggttctctcc ctttccgcta acaagacttt gacaaggccg
     4681 ccagctaccc gctaaatacc tcctttttga aaatttctca aaaaattttt ttggggacag
     4741 ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc aaacctaccg ggcgagaaaa
     4801 tgctttgcta tgctgctgat ttacatggta tagtacatac tcataaccgg aatagccctg
     4861 t
//
LOCUS       DAHEYX010000187         4847 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHEYX010000187 DAHEYX010000000
VERSION     DAHEYX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.188"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..269
                     /locus_tag="MB219_002303"
     CDS             <1..269
                     /locus_tag="MB219_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428003.1"
                     /translation="FQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHL
                     IEFMYQHPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(435..1628)
                     /locus_tag="MB219_002304"
     CDS             complement(435..1628)
                     /locus_tag="MB219_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8428004.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            1852..2229
                     /locus_tag="MB219_002305"
     CDS             1852..2229
                     /locus_tag="MB219_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCC8428005.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2217..2555
                     /locus_tag="MB219_002306"
     CDS             2217..2555
                     /locus_tag="MB219_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCC8428006.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2689..3132
                     /locus_tag="MB219_002307"
     CDS             2689..3132
                     /locus_tag="MB219_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCC8428007.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3394..4209
                     /locus_tag="MB219_002308"
     CDS             3394..4209
                     /locus_tag="MB219_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCC8428008.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4219..>4847
                     /locus_tag="MB219_002309"
     CDS             4219..>4847
                     /locus_tag="MB219_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCC8428009.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYF"
BASE COUNT         1608 a          852 c          926 g         1461 t
ORIGIN      
        1 tgttccaaaa atatcctgat aaggtcattt tgaagaatga gaataatcgg cgcttttcta
       61 tggaaaagca ggagaacttc ccaatctaca ttatttatct tcatttctac agaaaatcat
      121 ataattatca tttgattgaa ttcatgtatc aacatcctga gaaaaatttg gaagattatg
      181 cagacgctca atatacgagt gtatccacag tttttcgtta tgctaagtta ttagtaaagt
      241 actttgaaat gacggacgtc aaagaatagt agccgccaga tagaaggaaa ttagcagttg
      301 aaaaagctgc cctgcatcaa gtaatacgac cagccaaacg gaaacagtgt ctcatgtttt
      361 tgatttggct ggtcttatta ccgcagatgc aagcttttgc cacacctctt tcttgtcatt
      421 tacagactca actatcaatg ctgcaagctt tcccttaatg ctgtaacacc agtaagagtt
      481 gtcaagacca gcagactgac taggtaaata ccgctcaaaa acaagattac ggcagttaaa
      541 ctataatgat ccatcaagaa accgatgacg atagaagaaa aaccaccaac agctcgtcca
      601 aggttcataa tgaaattatt cgccgtcgcg cggatttctg ttggatacaa actactaatc
      661 actgcgccat atccaccata catgccattg ataaagtagc cgactatcac agcggctgct
      721 accaaactcc actggctatg ggctaacaca agtagataga cagataagga tgaacaaatt
      781 aaaaacaaag aaaaggctag tttaggcccc aatttatcca tgattatgcc aaaagtcatc
      841 atccccaaag acatgcctaa aatagtactg accatccaca atgaagaccc agaaaccgaa
      901 agccctaatc gattttgcat aattgaaggc agccaattca ttaacccaaa atagccggca
      961 atttggacag ttaccatgat actaaggcca catgtttgcc aagcaagatg ttttgattca
     1021 aacagtctgc taagtttggt tttcttttcg gcagattgta cagatttgaa ttcctttggt
     1081 tcagtcaagc cgcgacgaat gaacaagacc atcaacaccg gcagtacacc tacgacatac
     1141 aacatcttcc aaccaaacgt tggaatgatc acagcagcta aaatcgcagc caaagccgca
     1201 ccgacctgcg caccaatacc agcaaccgaa gaagctcgtc caatttgttt ttttgaaaaa
     1261 ctttcggaaa tcaaggacat acacgccccg tattctcctc ccgctcctac acctgcgata
     1321 aagcgaaaga gataaaccaa gtagatgttt gaagcaaaat acatcaataa agaagcaatc
     1381 gaaaacagaa ggaccgtttg agaaaatact ttcacacgtc catatttatc agctatgatg
     1441 ccaaaaaaaa tacctccaat gagcatcccc aaattagtga tcgttgaaat gaaacccgct
     1501 tgcgtaccac taatatgaaa agaagtaatc attgaagata atgaaaaagc taaaaacata
     1561 atatccatat tctctaaagc aattcccgcc gatgtcgata ataataactt tcgctgatac
     1621 gttgtcattt ttatacccct tttcctaaaa tttccctaaa ttgttaaaag tatatccaaa
     1681 aaagaataaa aaaacaagac ttttttattt gaaataatac acaaacaacc gatatagata
     1741 atccagctta gttttaatag aaatgagtat tgacttggag tacacttaaa gttgtagaat
     1801 aaataaaata cataagtaaa gtggcaagaa attaagagaa agagtgatca gatgaatatc
     1861 aaacaagtca gtgaagaaaa gggcatttcg gccgatacat tgcgctatta tgaacgaatc
     1921 gggttgattc caccagttaa tcggacaaaa ggcggaatcc gtgattatac agaagaagac
     1981 ctaaaatggg tcgactttac gatctgtatg cgaaatgccg gattatcgat cgagtctttg
     2041 attgaatata ttcgcctatc tagcgaagga gaagcaactg tatttgagcg gcgtaagctt
     2101 ttgattgaag aaagtgaact tttgacgaaa aaaattgctg aaatgcaaga atgtttggaa
     2161 cgtttacaag gaaaaatcaa gaaatatgat cgtgtgttat ctagaaataa aatggaatgt
     2221 atcaagtaaa aaaatcaagg agcatcgcta tggagttacg cttactaaaa tatttttgga
     2281 caattgcaga agaaggaaca atctctcaag ccgcagaagt tctacatctt acacaaccaa
     2341 ccttaagccg acaactgaag gagttagaag aggaactagg aacagaactg tttgtacgcg
     2401 aaaaccgcaa aatgatcttg acagaagcag gttattttct aaaaaagccg agcagaagaa
     2461 attttagatt taacgaatca aaccactcaa gaattcgaga acagaaaaaa acaattattc
     2521 agcggccgat ttacgatcgg ctgtgtggaa gctgataatt cgaatacatt ggcactatta
     2581 ctagaaaagt tgatccgtga ttatcccagc gttcaatttc atatccacag cggaacaagt
     2641 gatgatatca cggagtgact agaaaaaagc ctgttagatt tagcgatttt gctagatcca
     2701 gtagatacag agaaatacga aacgattttg ttaccaagaa aggaaaaatg gggattgctc
     2761 attttagaaa attctgtttt agcaaaaaaa gacgtgataa caaaagaaga ctttgcgaat
     2821 gtacctcttt tgatctcaaa gcgtccagaa atacagcgaa tgtttgctga ctggttaggc
     2881 ataaaaaagg agcagttgct gatcacagga acgtacaatt tgattttcaa tgtatttgcg
     2941 ctaattgaga atcaggtagg tgctgcttta gtcatagaag gtgctgtaag tcatctttca
     3001 aagcgagtga cattcgttcc gcttgaccca atgattgaaa cgaactgcat gctagtctgg
     3061 aaaaagaatc ggattttatc acctgtagtc aatgaattga ttcaccgttt caaagaagct
     3121 tttgaacagt aataagtatc tctaatcgaa gaaatttcaa tacaaaagcg agacgtatga
     3181 cagtcttgct tttgtatttt ttcattttta aatcaaaaaa aataaagaga tttacataac
     3241 ttgttaaata agcgcttaca aacataagaa gaacggaaat gaatcagcta taataaccgt
     3301 tgttctggat tgtaatcatt acaaagaaat gaagattata actttggagg tgttcgaaat
     3361 gaaagaaaaa aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg gttgggtatg
     3421 attctgctga ttgtattagc tttattgacg atcattcctt ttttatggat ggtttctgct
     3481 tcattcaaag caaataatga agtgttcacg attccgattc aatggatccc taaaacttgg
     3541 catttagaga attatctggt tatttggtct cgtattcctt tagtgacatt ttttaagaat
     3601 acagcttttt taagcgttgt gatcacgttg attcagttgt ttacttctag ttttgctgct
     3661 tacgggtttt ctaaactaaa ttttaaaggg cgcgatattt tatttttagc atatatcggc
     3721 acgattgctg tcccttggca gtcttatatg atcccgcagt tcattatgat gagaaaagtg
     3781 ggtttaacat atacgttgtg gtcgttggtg ctattacaag cgttcaatgc ttttggtgtg
     3841 ttcttgttga agcaatacta cagtagtatc ccagattctt tatgtgagag tgcgcggatc
     3901 gatggcttat cagagtgggg aatctatcgc aagatcattt tacctcttac aaaaccggcg
     3961 attgccagtt tgacgatcat cacattcgtt aatacatgga acgattatat ggggccattc
     4021 atttatttgt cttctacgga aaataaaaca atccagttag ggttgaaaat gtttattggg
     4081 ctatttgatg cagagtacgc attaatcatg gctgcctcag ttgtttctgt tcttcctgtt
     4141 gcttttatat ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc atcaggaatg
     4201 aaggaatagg aggtgattat gaaagagcag cgttttgaca aaaatgttta tatggaattc
     4261 ttttattggt tgtatcaatc attggcaagt agcctttgtt tttggtgtgt caatatcttg
     4321 tatatagcag ctgcgttcct tttgtcgata gatggaagga atgtcttttc ctttattttt
     4381 tctttacttt ttattggtcc aggttgcatc gtgatgatag gtatgacaga agaattagca
     4441 aaagaaaaaa gtttttcacc agtacgtttg ttcataaaga cgtttcgcca attttggcta
     4501 aagggatttt tttattggct atttgcttgg atagttagtg tgataatgat ttttgattgt
     4561 ttctttttta tacgtttttc ttatggaaaa tggttgattc cgttgtttgt tttacttgct
     4621 tgtttatcag tttcctttag catcaactac tggtattttc aggtgagaaa tccagcgtcg
     4681 aaaccaaatc aagttctacg gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt
     4741 ttactagatt ttttactatt gacaagtttg tttctcttct ttttcgtaaa gccccaatgg
     4801 tgtattctac taggtccttc tatcgtattt ggtttgattt acttcaa
//
LOCUS       DAHEYX010000188         4838 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHEYX010000188 DAHEYX010000000
VERSION     DAHEYX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.189"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="MB219_002310"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="MB219_002310"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCC8428010.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="MB219_002311"
     CDS             2714..2938
                     /locus_tag="MB219_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428011.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3454
                     /locus_tag="MB219_002312"
     CDS             2951..3454
                     /locus_tag="MB219_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCC8428012.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            3581..4618
                     /locus_tag="MB219_002313"
     CDS             3581..4618
                     /locus_tag="MB219_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428013.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            4633..>4838
                     /locus_tag="MB219_002314"
     CDS             4633..>4838
                     /locus_tag="MB219_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HCC8428014.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEK"
BASE COUNT         1774 a          696 c         1009 g         1359 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacggga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcctactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tgcgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc tgtcgatttt gatgaagtga aagaacagat
     3061 tggattaaat gacgaatatg aagagtacgc gattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acgccaattg aagagattaa tcggttgtgt aacttagcgg aggaactaga
     3181 aggaacgcca attggtgaag ctgcttctga gattcaacac gctttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatattgt ctattatcca gattgcgatg atatgagtga
     3301 tgtggcgcat tacttgattg tagaagctgg tgattttggc gaagttccag aacaattgaa
     3361 gccgtatatt gattttgatg cttgcggtcg agatttggag attggaggaa attatttggt
     3421 tactagtcgt ggagtttttg agtatccaga atgaatcaaa cactatatat agtgtttgat
     3481 tagtatatgt aagcaaaaaa acacaatata tagagtgcaa actgaagggt aatgtgatat
     3541 aatatttgcc gaagaaatat ttttgaaatg aggtaatatt atggcaaata ttcaaacaag
     3601 ttttattgat ttccataatt caattagatt ggacgtagaa gacaatacgc tacttaagga
     3661 ctataaagac caagtaattg atggattaaa agattactta cctgatgatg tgaagtttga
     3721 aactttttta cagggaagct attctgttta taccggaata aaaagctgtg atgaaaaaat
     3781 tgattttgac atcgatattg cagttgcttt cgaaattgat catactgttt atgaagatcc
     3841 aagggagcct aaactgtggg taaaagaggc gctagttgaa atttttccta atgctcaagt
     3901 taatttaaaa gtaccttgtg taactgctac atttactgga aaaaagacaa aaaaaaatgt
     3961 gcacgttgat gttgcggttt atgctaaaga agatgaaaat tatttcttag caaaagcaaa
     4021 agaattcagt gctcccgaaa accgatgctg ggaggaagcg gatcccaaag tattaaaaga
     4081 gaaaattaat agtcatgtag cagattcaga tgatcgaaaa caatttcgaa gatgtattcg
     4141 ctatttaaaa agatggaaag ataacaattt taaccaagaa tataaaccaa caggaattgg
     4201 acttacaatt aatgtgatgg atacgttcct agttaataag tccactgatt ttttaactag
     4261 aaaagttcag tataatgata tggaatgtat gaagcagatt gtttcttcgc tgaaagattc
     4321 atttgtgtat gagtacagtg aaacagatgg ttggcattat cgtctacatg catatcttcc
     4381 agtgaagcct aacagtgaca cctattcaaa aatgacagtg aatcagatga gtgattttaa
     4441 aaacaaactt tctaaattat atgatgattt gattttcgct atcgatactg aagatgagta
     4501 tgaagctaca aaacgattaa ataatcaatt tggggaggat tttttaatta tttcagaaga
     4561 agaagttaca gaaaaaaact taagaaatgc atttgttaca gactatccga gtgcttaggt
     4621 tggtgttaaa gattggatta tactgatatt ttaaaagaaa agctaacaga tgttgtgcgt
     4681 ttattcgatg gtaattacta tggggatttt tcatttgcta caattggaaa gttgcgttta
     4741 aaaattgaaa taccaaaaca ttttccattg gtacttccta ggatttacat tcataatttt
     4801 gatcatttag gactttacct accacatgta gaaaaaga
//
LOCUS       DAHEYX010000189         4806 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHEYX010000189 DAHEYX010000000
VERSION     DAHEYX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.190"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..411
                     /locus_tag="MB219_002315"
     CDS             <1..411
                     /locus_tag="MB219_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428015.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="MB219_002316"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="MB219_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HCC8428016.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /locus_tag="MB219_002317"
     CDS             2446..3708
                     /locus_tag="MB219_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCC8428017.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4806
                     /locus_tag="MB219_002318"
     CDS             3953..>4806
                     /locus_tag="MB219_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCC8428018.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaaa
//
LOCUS       DAHEYX010000190         4687 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHEYX010000190 DAHEYX010000000
VERSION     DAHEYX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.191"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(29..1177)
                     /gene="nagA"
                     /locus_tag="MB219_002319"
     CDS             complement(29..1177)
                     /gene="nagA"
                     /locus_tag="MB219_002319"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCC8428019.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1253..2089)
                     /locus_tag="MB219_002320"
     CDS             complement(1253..2089)
                     /locus_tag="MB219_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428020.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2273..3490)
                     /locus_tag="MB219_002321"
     CDS             complement(2273..3490)
                     /locus_tag="MB219_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCC8428021.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3639..3818)
                     /locus_tag="MB219_002322"
     CDS             complement(3639..3818)
                     /locus_tag="MB219_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428022.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3873..3956
                     /locus_tag="MB219_002323"
     tRNA            3873..3956
                     /locus_tag="MB219_002323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3907..3909,aa:Leu,seq:gag)
     gene            complement(4196..>4687)
                     /locus_tag="MB219_002324"
     CDS             complement(4196..>4687)
                     /locus_tag="MB219_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428023.1"
                     /translation="ALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTEL
                     GTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGF
                     SPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITV
                     INR"
BASE COUNT         1402 a          986 c          761 g         1538 t
ORIGIN      
        1 tttagtttta aggttaactt aaattatatt atgcgtggaa acgttccacg ccatctagat
       61 aagttgcttc caatgtcata tcagggttca atacgataaa gtctgcatcg cgtccttgag
      121 cgatttttcc acatgtatca tcgattttac agctgattgc tggaatcaga ctagccatca
      181 tgactgcttg ttctggtgtt gctaatcccc agtctacaac atttttaatt gcttctttca
      241 atttcaatat acttccagct aagttgccag attctagacg tgctgttcct tcttttacta
      301 ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat cccaccggcc atcatacagt
      361 ctgtaattaa tgcgacatga tcatgtcctg ctttttccat taggattccc gcagcatttg
      421 gatgtacatg atggccatca caaatcaatt cagaaaagac atgttctaaa gataataacg
      481 cacccaccat acctggttcg cgatgattca atccacgcat accattgaaa gcatggacaa
      541 atacacttgc gcctgcttca acagcttttt gcgcttcttc tagtgtagca ttactgtgtc
      601 ctagagaaac aactactcct tcttcagtca cttgtttgac aaactcttct acaccttgac
      661 gttccggtgc taaagcaatt tttttgatta aaccacctga agcttcttgc cattcatgga
      721 aagtatccaa atcaggatcc ccgaaataac ttgggttttg tgcccctttg tgttcttctg
      781 tgaagaaagg accttcaaaa tagattcctt tgattttagc tcctggtgct tcctgataca
      841 ttttgccgat tgtctcagct acatctttta aacgttcttt gcttgacgtc aatgttgttg
      901 gtaaaaatga agtcactcca caagataaca gaccttcaga catcactttg atgccttctg
      961 catcattatc cattacatca tgattcatat agccgtgaat atgtgtatct accaaaccag
     1021 gagcaatcca tttgccgctt tggtcaatga tgttgacttc tccctcaggt agttctttgg
     1081 tgtattcacc aaaaagtcca tcggtaattt caagatagcc tgcatgtttg acacctgacg
     1141 ctaaaaagaa tttgtcagca tagataaaag ttctcattct attctctccc tttgtttttc
     1201 tacctctaaa gtactcttat ttagagaaga agtcaagaat ggtctacacc atttacaaat
     1261 tttttgaaat ttgtttcaca gcttgtgcta agccttcata gaatgctgct ctttctggtt
     1321 cttctttacg cttgtatatt aagcttttaa gcacaaataa atcattgagg taatagtagc
     1381 tattcttctc ttgagcccaa tagattcctt gattcacctg ttctaacgct tcgtcgattt
     1441 cattttgatg gaataagaaa tttccataat tataaaaaat ttgcgaaaat tcactttttg
     1501 atcgttcatc agctgattga tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt
     1561 cagccgtttt tcctgccaca ccatatattt ttccgataca actaatcagt tgtatttctg
     1621 tgtcagaaac atggattctt tctttttggc tagagtagct taatgcttct ttcaaataat
     1681 aaagggactg agagatattt tgacgcaacg tgaattcgca aacaccgaga taataataat
     1741 agcgttgaaa gtcagttgct tgatacagat tttcagaaat atcagaagat tttagtactt
     1801 gttctaactt ccgataatca cgtttttgga aacaactatc caacagttca aataatttat
     1861 cgtttgtccg agtagttttt cgagagagat tcatcacatg atcaatcgtt acccctagcc
     1921 gttgacaaat ctgcatcatc accaatacat tcggcagttc ctcattattt tcgatccgac
     1981 ttaatacact ttgtgaacaa atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt
     2041 ttcgaatctc tttaatgatc gttccaaagg actcgattaa cgtttccata agtatcctcc
     2101 ccaaaatatg cgtattcgca ttctattcgt attttacttt aggaagtcgc aaacttcaag
     2161 aaaagacttg caactaaata atatacttta gttatttaag aaaactccac tagaaagaga
     2221 gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt tggtttttaa aatcattttt
     2281 ggcgataggt tgctaggaat tctctcattt tcttagctgt ttgtttcaaa tcatccattt
     2341 taggaagata aacgattcga aaatgatctg gctggttcca attaaatcca ccaccatgta
     2401 caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat
     2461 tgaatcgctt tgtatcaatt tttgggaata tataaaaagc agcttttggt tttacggctg
     2521 aaagaccagg aatatcattg attgcattat agatatattc tctttgttca tagatccttc
     2581 cgcctggcag gagcagctca tcgacacttt gataaccgcc tagcgcggtt tggataattt
     2641 gctgagacaa tacattggaa cacaaacgca tggaggacaa catattcagc ccttctatat
     2701 agcctttgac gtgtgactta tctccactta ggaccatcca gcctactcga aatcctgcta
     2761 cacgatgaga tttggataga ccgttcagcg taacaacgaa caaatcaggc gctaatgtag
     2821 caatcggaat atgttctaga ccatccatca ctaagcgatc atagatttcg tcggaataaa
     2881 taatcaggtt attttgtcga gctacttcaa cgatttcttc taataattct tttggataaa
     2941 gtgccccagt aggattatta ggattaatga taacaatagc ttttgtccga ctcgttattt
     3001 ttgatttgat atcatcaata tcaggatacc attctgcctc ttcatcgcaa atataatgaa
     3061 caggtgtgcc tccagctaat gcaatagaag ccgtccacaa cggatagtcc ggcattggaa
     3121 ccagtacttc atctccattg tccagcaacc cctgcataca catcgtgatc aattcgctca
     3181 ctccgttgcc ggtgtagata tcattgatcg tcacattcgg aaaatgtttc agttggcaat
     3241 attgctcaat tgcttttcta gcagaaaaga tccccttgga atctgagtag ccttctgagt
     3301 cacgggcatt catgatcaaa tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg
     3361 cgggatttcc cgtgttcaat tttaaaatac taatgccctc ttcttgcatc cgctccgctt
     3421 cttctagaac gggaccgcga acatcgtaac tgacaccttc taatttgttg gatttttcaa
     3481 attctctcat cacttagcca atccctcatt tcaaaaagtt agaactttcc aaattttttg
     3541 ttatttacta tttcaatatc atactccatc ctagaaaatt ttgccactta tttaggaaaa
     3601 acgtaagtat taaaaaaaca caaacaagcg ttgagaactt aattgtcatg atagtcattc
     3661 catagattcg ctgcccaatc cattgcaatt ccacaaatga ttcctagcat aaaaccaagc
     3721 aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga tcattcccag tcctgaaaat
     3781 tctccgtcat atttctccat cttaataagt cctcccattt ttgagcttga caactatgta
     3841 aaacctcgct atcattagtg tgttattatt acgcagatgt gttggaattg gcagacaagc
     3901 atgattgagg gtcatgtgag cgttagctcg tgtgggttcg agtcccatca tctgcactac
     3961 aactcgtgaa gttaattttg cttcacgagt ttttttgttt gaacgtttct ttatgccatt
     4021 atatacatat cactgtatcc tttacaaaaa gaacgtacaa tcatgtaaac agcctgaggt
     4081 gagtatatga aggtcggaaa caagctactc gtagaaactt gcaagtgtta aaacaattaa
     4141 tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga gggatttttt gtatattatc
     4201 tgtttatcac cgttattata aaggtacaaa taccaagcac ggttaatcca aattgcataa
     4261 attcattttt ttgtacactc atgcttttca ttagctcttt aactgccatt agtactaaga
     4321 tggcgataac aacataaagt atatagtacg tccgcattcc ggaagcccat actgtgggag
     4381 aaaatcccat aattaacctt gaacaaaatc ccattatcat caaaataact aaaaaaatag
     4441 aagttttctt attgtcaaaa cttaaataaa ttccaacaat taaacatagt aataatgcca
     4501 agactactaa agtagctatc caagtacctg gataaaaaag tgacagtttc gttcctaact
     4561 ctgtaaagag attattcagg ttactactac tccaaatcat tgccctttta tttccattga
     4621 cgtaagtaaa gctttgaccc attgtattac caaaataaat aatcaggttt aagaaaaacg
     4681 gtaatgc
//
LOCUS       DAHEYX010000191         4636 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHEYX010000191 DAHEYX010000000
VERSION     DAHEYX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.192"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..519
                     /locus_tag="MB219_002325"
     CDS             <1..519
                     /locus_tag="MB219_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8428024.1"
                     /translation="FAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHRYQT
                     ELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLTYHR
                     LMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQENE
                     IIYLAIHLARIY"
     gene            623..1387
                     /locus_tag="MB219_002326"
     CDS             623..1387
                     /locus_tag="MB219_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCC8428025.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1374..2816
                     /locus_tag="MB219_002327"
     CDS             1374..2816
                     /locus_tag="MB219_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCC8428026.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            2827..4157
                     /locus_tag="MB219_002328"
                     /pseudo
     CDS             2827..4157
                     /locus_tag="MB219_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4170..4628
                     /locus_tag="MB219_002329"
     CDS             4170..4628
                     /locus_tag="MB219_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCC8428027.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1577 a          699 c          979 g         1381 t
ORIGIN      
        1 tttgctattc aacgaaaaag agaagataga gatgtcacag caattatgtt gccagaaatg
       61 agattccttt atccaaatga atatgatata gcaatagaag tggtcgagcg aatcaatcat
      121 cgatatcaaa ccgagttagg gtcaaatgaa gtaggattca tcactatgca tatcattaat
      181 gctgagctag gagagcctaa tagtttaaag agcttaaaaa tagtagaaat gacgaccacg
      241 attttgtccc atctagaaaa atcctattca aacgtttatg ataaagaatc actgacttat
      301 catcgcttaa tggttcatat caaattcctg gttaaacgct tgatttatca ggaagaaacg
      361 ccagtagaaa atattggttt ttttactgaa acatttaaac atagcaaagc gtattgtaca
      421 gcactaaaga ttaaagatat catcgaacag caatttcaac ttgccattca agaaaatgaa
      481 atcatctatt tggcaattca tctagcacga atctattaaa ataagggcgt aactatctat
      541 aggcgcaacc aaatgacaca tagtaaatgg actatgatta tttggttgcg cctattttag
      601 tagaaaaaag aaggagaatg aaatgggaaa agtgattttt aattcggatg attttggcta
      661 tagccatggt gtgaattatg ggattatgga tgcttatcag cgaggaattt tgacatcaac
      721 tacattgatg gcaaatatgc cgggtttcga gcacgctgtg aaactaagaa aggaaatgcc
      781 taggctaggt gtaggagtcc atttaacatt gacgtgtggc aaaccacttc tcaaaactgt
      841 cgatactctt atcgaaagcg gagagttcag atccttatct tattatcagc atccatttaa
      901 cattgacaaa gatcaactgt atcaggaatg gaatgctcag attcaaaaaa tttatcatgc
      961 ggggatcata ccaactcatt tagacagtca tcatcatact catactttgg atgaaaacca
     1021 ggaggtagtt gttacattag caaaaaaata tgatttgccg gttcggagca actttgaacg
     1081 aaaagatgaa gttcgtcatg tcgattattt tgagccttgt ttcgatgtcg taggagaaac
     1141 tgatgaaaaa aaacgacaaa tcaatacgag tgtggaagaa tatttggcaa gtttactcat
     1201 gatattgaga gaagataaaa cgatcgaagt catgtgtcac gcagcgtatt tggatcagga
     1261 attgttaaat ggttcaagtt ttgtgtatcc tcgtactcac caagtcgaat ttttaattca
     1321 ttcagcattt gctcaaaaag tgaaaaatga tcctttgatt gagttagtaa catatgagga
     1381 tatttaatag aggaggaata aaaatgaatg ttaaatcaaa tgtgcagaag cttggaagag
     1441 cgatgctact tccagttgca gcgatgccat tagcgggatt gatcatgcgt ttatcagctg
     1501 atgatatgct gaacatccca gtaattggtg cagctggaaa tgccgttttt ggaaacttgg
     1561 atatcttatt tgctatagga gttgcaatcg gttttgtcaa aaataaggac aaaggaatac
     1621 ctgcacttac tggtttttta gcgattgcta ccttgaaaaa aggcttagaa atcatggacc
     1681 cagaagtgaa tatggggatt tttggtggaa ttatttccgg tttagtagcg gcatggacgt
     1741 ataatcgctt taaagaacaa aaattaccag tagttttttc ttattttgcg ggcgaaaaat
     1801 tcccattgac tatggtgatg attctacaaa caatcgcagc tatttttttg ggctttatct
     1861 ggccgctcat ccaaattggg attgataatt ttgcaaaact gctcgttgat atgggggcat
     1921 taggtgtggg gatatttatg tttttgaatc gtttgctgat cccctttgga ctgcaccatg
     1981 tgttgaatac gtatatttat tacgatttag gaagctatac atctcctagc aaggaagttt
     2041 tcagaggaga gatgacacgc tttatcaacg gagatcctca ggctggactg tttttatcag
     2101 gtttttttgt agtaatgatg tttggtatac cagctatatg tctagctatc tataaagcag
     2161 cttataaaga aaataaagag atggtcaaag gaatcatggg aagcggtgca ggaacttcat
     2221 ttattgcaaa tatcactgag cctgttgaat ttagtttcat gtttctttct cctatgttgt
     2281 acgtgatcca tgcacttttt gccggtttag cagggattgt ctgttactta ttcaatatac
     2341 gaattggttt tacattcgga gcatgtattg ttgattatct catcaatttt cgaatcgcta
     2401 ccaatgctat tttgattctt ccaatcggaa ttttcttttt tgccttgtat tacgttactt
     2461 tttattattt gatcaataag agaaacattc agacattagg acgtgaagct aaggcagagt
     2521 ttggcaatga agtaacctta gaagaaacag agttagggtt agcaagtaaa aattatgaat
     2581 atatggcaac aaaaatgcta caagcgtttg gaggaaaagc caatatcctg gatgtctata
     2641 gttgtaatac acgcctacgg gtagaggtag tggatcccac catggtcgag gaacagcgaa
     2701 tcaagcaatt agggataagc gggatcatta aaccaactga aaaaaattat caaattatca
     2761 tcggcttaga agtgacttat gttatggcag aattcaataa attattagaa gaatagaaag
     2821 gaaaagatga tgatgaaaaa acagattatc actatagctg gaggcgggag tacgtataca
     2881 ccaggaatcg ttcaagccat tttgaacaat gaaaaacggt tgccattatc tgaaatacgt
     2941 ttatatgata ttgatgaaga aagaaatatg gatatgtact tgattgttaa atttatgctg
     3001 aaaaaaaagg tttttcaaat attcgcattc gtgcaacgga tgatccaaag cttgctttta
     3061 caggatgcga ttttgttttc tcacaaattc gtgttggagg attggagatg cgagaaaagg
     3121 acgaaaagat tccacttaaa catggattag taggacagga aacgtgtgga ctcggaggat
     3181 ttgcttatgg gatgcgctca atcaaaggtt tattggaaat tgtcgatcat atacaagact
     3241 atgcccccaa agcatggatt ttgaattata caaatccaga gtcgatcgtt tctgaagctg
     3301 ttcgtcgtaa atatccgaat gtaaaaatga ttaatgcctg tgatatgact atttcaattg
     3361 aagaaacgat tgcagtgaat tacggttatg atcgtaagaa ttggatcgtt acgtattatg
     3421 gtttaaacca ctttggctgg tatacatcga tttatgataa agaattgcaa cgagaaatta
     3481 tgccagagat tattgaaaaa ctaataacaa aagaaatgca agtcgccgat tttaatatag
     3541 gagacaaaac ttggcaaaaa acctttcaaa tgatgtcagt catcacgaaa aattttccaa
     3601 gtaatatccc gaataattac ttagagtatt acttatatcc tgatatggtc gttgagcata
     3661 ctgacaaaga gtatacacgt gctaatatgg tcatggatgg acgagagaaa aataccaaag
     3721 aaatggctga taaaatacgc cgcggtattg tggaagaagt attgaacttt aattttgggg
     3781 agcatggaca atatatcgtc gatatagcta tttccttgtt gaatgatgat cgtcgtcgct
     3841 ttatgctaat cgttcctaat caaggggcga ttccgaatct acggtcagat gcagttgttg
     3901 aaataccggc atatgttggt gcaactggtg tggagacaat tacactaaga aaaccaatca
     3961 gtgattttca taaaggattg atggaggcac aagttgcagc agagaagtta ctagtagatg
     4021 cttactttga aggttcttat caaaaagctt tacaagcatt tacattgaat caaaccgtac
     4081 caaatgccag agtcgcaaaa aaaatactag atgaaatgat caaagtaaac aaaaaatatt
     4141 ggccagcatt aaagtagagg tgataaaaag tgcatttctt taaaacaaga aacattctaa
     4201 aagctgtagc tagtggtcaa attattccat taacagcagt aaaagacgag gtcttttctt
     4261 caaaaatgat aggagttgga tacgctataa cgaatcatga tggatatatc catgcgccag
     4321 ttaaaggaat aatcgataat atatttccta cccgacatgc cataacgatc gaaagtgaat
     4381 ctggcagaaa aatactgatc catatgggtt tagatacagt tgaactcgaa ggaagtccgt
     4441 ttcagatacg ggttgaaagt aaccaggagg taaaagaagg gacactgtta gctattatag
     4501 atttgaagaa gttggagcaa gcacaaaaag atacggtgat cattgtagtt tctccaggca
     4561 tttttgaagg aaatttatta aaacaaaacc aagaagtaaa gattaaagat agttgttttg
     4621 aattttagtg tacgat
//
LOCUS       DAHEYX010000192         4484 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHEYX010000192 DAHEYX010000000
VERSION     DAHEYX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.193"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            47..1123
                     /locus_tag="MB219_002330"
     CDS             47..1123
                     /locus_tag="MB219_002330"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCC8428028.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            1276..2628
                     /locus_tag="MB219_002331"
     CDS             1276..2628
                     /locus_tag="MB219_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCC8428029.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            2950..4461
                     /locus_tag="MB219_002332"
     CDS             2950..4461
                     /locus_tag="MB219_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCC8428030.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
BASE COUNT         1558 a          854 c          969 g         1103 t
ORIGIN      
        1 atgagagtaa atcactgaac gatttagaat acaggaggac aatcttttga agattacttt
       61 actatatggc ggacgcagcg cagagcatga agtgtccatt ctttccgcat tttcagtttt
      121 aaatgccatt tattataatt attaccaagt tcaactcgta tttattacaa aagaaggaca
      181 atgggtcaaa ggtccattac taacagaaaa acctgccagc aaagatgtct tgcatctttc
      241 atgggaccca agtggacaga cagaggaagg ctttacagga aaagtgatca atccgggcga
      301 aatcaaagaa gaaggagcca tcgtttttcc agttttacat gggccaaacg gggaagatgg
      361 aacgatccaa ggcttcttag agacattgaa tatgccttat gtcggcgcag gcgtattgac
      421 cagtgcatgt gccatggata aaatcatgac caagtatatt ttacaagctg ctggtgtgcc
      481 gcaagttcct tatgtaccag tacttaagaa tcaatggaaa gaaaatccta aaaaagtatt
      541 tgatcaatgt gaaggttctt tgctttatcc gatgtttgtc aaaccggcga atatgggttc
      601 tagtgtcggc attacaaaag cagaaaaccg agaagagctg caaaatgctt tagcaacagc
      661 ctatcagtat gattctcgag caatcgttga acaaggaatt gaagcgcgcg aaatcgaagt
      721 tgctgtatta ggaaatgaag acgttcggac gactttgcct ggtgaagtcg taaaagacgt
      781 agcattctat gattatgaag caaaatatat caataataaa atcgaaatgc agattccagc
      841 cgaagtgcca gaagaagttt atcaaaaagc gcaagagtac gcgaagttag cttacacgat
      901 gttaggtgga agcggattga gccggtgcga tttctttttg acaaataaaa atgaattatt
      961 cctgaatgaa ttaaactcta tgccaggatt tacggagttc agtatgtacc cactcttatg
     1021 ggaaaatatg ggcttgaaat acggtgattt gattgaagaa ctgatccagt taggaatgaa
     1081 tcgataccat cagcgtcaat ctttttttga aaaaaatgaa taaagagaaa taaagaagag
     1141 gctggagtga ttgcgtaacc gcgttcattc tagcttctaa atacgaatag agaaatttac
     1201 ttggaagtga gagtcatgcg aacccgccgt ttaggccaaa cgcttttttc acaacctttt
     1261 ttaggagaga tctcaatgaa tctaacaata caagaaattg cacaagccgt gggcagcgaa
     1321 tgctctgcac tgacaaagat taccaatgtc gaatttgaca gccggaagat aaccgaagga
     1381 agtttattcg ttcctcttgc tggtacgcga gatggacatg aatttatcca gcaagcgatc
     1441 acaaacggag catctgcttc attttggagc ttgcctgttg aacaagctcc aaaaaatttt
     1501 ccagttattc ctgtagcaga tcctttgaaa gccatgcagc aattggcaac ctattacttg
     1561 gaaaaagtag gcgcagaagt cgttgcaatt acaggaagca atggtaaaac caccacgaaa
     1621 gatctaaccg cgtctgtctt gtctgaaaaa tacaaaacat ataagacgca aggaaattac
     1681 aacaataata ttggtcttcc ttacacgatt ttgcacatgc cccaagatac agaaaagatc
     1741 gttttggaaa tgggcatgga tcacgccaat gaaatcacgg aactttcact tatggctcaa
     1801 ccaaaagctg cagcaatcac aatgatcggt gaagcacaca tagaaaatct tgggtcgcga
     1861 gaaggaatcg ctaaagccaa aatggaaatc actgacggac ttgtatcaga cggattactc
     1921 gttgtgcccg cagatgaacc gttgctgaca cctttaacgg caacattagt acaaacagtc
     1981 acaacattcg gtattggaaa tggcgatgtt catgcggaag tgacagcaaa aggaaaagca
     2041 caaaccgatt ttgtagtaga aggagaaaca tttacgatcc cgctgcctgg aagttataat
     2101 gtaacgaatg ctttgattgc gtatacgatc gggcgcttct ttggactgtc catacctgag
     2161 atacgccaag ggctcgccca tgtttctatc actcaaaaca gaaccgaatg gctaacggca
     2221 ggaaatggtg cagctatttt gagcgatgtc tacaatgcaa atccaacagc catgggactg
     2281 gtattagata cttttaaaaa tctgccaaca aaaggacgca gaatcgctgt gttagcagat
     2341 atgctggaat tagggaaaga gtctttaaat atgcatgcgc aaatgagtga acatatcgat
     2401 cctgagaaat tcgccatgat tttcctttat ggggaacaaa tgcatgcact aaaagaagaa
     2461 ttgaaccaaa aataccccca actacctgtg ttctattttg aaaaagaaaa gaataaattg
     2521 attgatgcta tcaaacaaaa tcttgcagca acagatagta ttatgctaaa aggcagcaac
     2581 agcatgggct tatttgaagt ggtagaacaa cttcagcaaa tgaaataaaa atctgtaaac
     2641 ttgcagaaaa cgaaaaacta tgttagaata acctattgcc gaaagatgaa gagaaattgg
     2701 actggttttg ccatagaaac aacttgttga agccagatgt cataataatg ataaagctga
     2761 acgcgacccg tttggctttt ctaatgccta aacttcgcaa agtgattttt cggtggtgcc
     2821 acaggggcta agaatggata aaaacgtgcg tggccactgt caaaaagaaa caatacactt
     2881 tgaccgaaca aatcgacggg gtagcaaaaa agctacatcc tcagttaaca accatatagg
     2941 aggattcatt tgaaatttaa agaactagaa ttatcaccag aattgttgaa atcagtcgaa
     3001 cgcgctggtt ttgaagaagc aacgcctatc caagcagaaa cgattccact agcattagct
     3061 ggaaaagacg ttattggaca agcacagaca ggtacaggga aaacagctgc atttggatta
     3121 ccaatgttag aaaaaatcga tccagatcgt cacgaattac aaggtttagt tatcgcacca
     3181 acacgtgaat tagctatcca aacacaagaa gaattatatc gtttagggcg cgacaaaaaa
     3241 gtacgtgtac aagccgttta cggcggagca gatattggcc gtcagatccg tggcttgaaa
     3301 gatcgtccac atatcgttgt gggtacacca ggacgtatgc ttgaccacat taaccgccac
     3361 acattgaaac ttggaaccgt tcaaacatta gttttggatg aagcagatga aatgttgaat
     3421 atgggcttct tggaagatat cgaaaaaatc atttcacaag ttcctgatca aagacaaaca
     3481 ttacttttct cagcaacgat gccgccagct atcaagaata ttggcgttaa attcatgaaa
     3541 aatccacatc atgtgaagat caaagcgaaa gaaatgacag ctgacttgat cgatcaatac
     3601 tatgtccgcg caaaagaata cgaaaaattc gatatcatga ctcgcttgtt tgatgttcaa
     3661 acacctgaat taacaatcgt cttcggacgt acaaaacgcc gtgtagacga attggcacgc
     3721 ggattagaag cacgcggtta ccgtgcagaa ggaatccatg gcgatttgtc tcaacaaaaa
     3781 cgaatgagcg tattgcgttc atttaagagc ggtcatttag atatccttgt agcgacagac
     3841 gttgctgcac gtggattaga tatttcaggt gtcactcatg tgtacaacta tgatatccct
     3901 caagatccag aaagctacgt tcaccgtatc ggacgtacag gccgtgccgg aaaaggcggt
     3961 atgtctgtga ctttcgttac accaaatgaa atggactact tgcatgtgat tgaaaacttg
     4021 acgaaaaaac ggatgacgac tttacgtcca ccaaccgaaa aagaagcatt caaaggtcaa
     4081 ttaggtgctg cagttgaaca aatcgaaaca aaacttgcag aaaatggtct agataaatac
     4141 ctgcaaacag cagataaatt attagaagaa tattctgcac aagatttagt tgcacttttg
     4201 ctaaaaacga cagcaaaaga tccagctgat gcagtgccag tgaagatcac accagaacgc
     4261 ccattgccaa tgcagaaaaa aggatacaat aaaaatggca aacgtggtgg cggaaacaac
     4321 cgtaatcgtc gtgacggcgg taattatcgt ggaaacaaat caaaaggcgg ttatagcaaa
     4381 aataataaca gccaaaaaga tggcggtaaa cgtcacaatg ataaaaaacg cggatttgta
     4441 atccgttcaa ataacgatta aacataaaat gaaagtttct cttt
//
LOCUS       DAHEYX010000193         4464 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHEYX010000193 DAHEYX010000000
VERSION     DAHEYX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.194"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            573..1007
                     /locus_tag="MB219_002333"
     CDS             573..1007
                     /locus_tag="MB219_002333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428031.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2297..3220
                     /locus_tag="MB219_002334"
     CDS             2297..3220
                     /locus_tag="MB219_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCC8428032.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3236..3775
                     /locus_tag="MB219_002335"
     CDS             3236..3775
                     /locus_tag="MB219_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCC8428033.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          667 c          756 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca ttggtagagg
      421 ggctacttgg catagtgtac attatgtcta ctaaccttta aaataacacg ttttataaaa
      481 taaaccctct tgacaatatt taattttctg attattctaa atatatacag aaagcgcttt
      541 cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaattatt aaaattatta
      601 cttgtctatt tttgttcggt atgattgttg gagcaagtac tccgttaaat ctgtttcctt
      661 ttagtgctag aggtataaaa gtggaagctg caagttcacg atataatcat aatcatcgtg
      721 gatttacttg ctataaaatg aactattatg ttactaataa aatgtgtaga gatttgaaaa
      781 aaaattataa taagctaaag actcctgctc aaattgcatc ttttattcct attggtggag
      841 ttggaacatg ggttattaca aatgctttta gtagtgctgt tgatgcaatg aatgtctttg
      901 taagagctgc aaatcaagga aaaggtgtac aattaacata taatgctcac tttagcaata
      961 caacatcata tcaatacaat gattacgctc gttatgtaat aaaataatca gaaagtgaaa
     1021 ctatctcatg aataaaaaga ttttagctgt aattatttca ataatagttt taatcactat
     1081 gttcttcata tttaggctaa ttttcaatat aagcttacaa aaaagtattc tttatctaat
     1141 tcctatagca tttatcttag caatttttag aagtatttac ggaaataaaa actctaaata
     1201 gctaaaaata aatcatacta aattttacaa aaagtggcta gcattttcta gccacttttt
     1261 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1321 cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct
     1381 tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc
     1441 gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt ggctagcaaa
     1501 aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa gtgagcgaat
     1561 ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg ccgacttttt
     1621 taaaagtcta ggcacacgag caaatatctt tatcaagctc taatttgcgt tttaagacgt
     1681 ttgaaacgcc taagtatata aatatataag aataggttta agagtgctta aatcgaattt
     1741 tagagggcat ttagacaatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgt
     1801 tttaagaaca agataaggga tttatactag gactgtctta aaatgtctta aacgaacgct
     1861 cagagcttct caaagggttc cttatgaacg gcttttagca aaggagccgg aggcgactgc
     1921 gcagtataat gtgagagcag agcgatcaca cctttttagc gaaccgtagg gagcaaggtt
     1981 tggaattggc agttttgata aattcgcgaa cctgtttaga gaaaaaagga gtgatttttt
     2041 aggcgggaaa attagcctcc cctcttttta tatctctttt tatatctctt tttatatctc
     2101 ttttatgtcg gctgtgtcct tactctccca agcaattcag agttttttca agtaggtttt
     2161 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc ttttcaagta
     2221 ggttttccga cattattgag tatttttata aagtatgtta cttttatttc atactttata
     2281 aaagtaggag aagcttatgg caaatgaaat tgtaaaatat gaaaatagat taaatcatat
     2341 tcctctgaga aaatttaata gtcgtgaaat gaacttattt ttttcaattg cttcacgtgt
     2401 acgtgaaaaa ggtgtagatg agattatttt ttcatttcac gatttgaaaa aactaagcgg
     2461 ttatacagaa cgtggggaac aatttataaa agatcttagt agcacgtatg acaaattatt
     2521 aagtattaat gcttggaatg atgatggaaa tactttaact aaatttgttg ctttcacaaa
     2581 atatagcatt atacgagata aacaacttgt tataatttca gttaatccag aatttaaagg
     2641 actttttaat caattaaaga actggacaag atttaatcta gagcaattta ctaatttgga
     2701 tagcacatat tcaaaaacta tgtttcgatt attaaaacaa tatcgaactc aaggctggac
     2761 tgaattttcc aaagaaaaat tttttgattt actagatatt cctaaaagtt attggaatag
     2821 tccgtcaaat attgacaaat ttatcttaag gccaattaaa gaagaactca ctcccttatt
     2881 taaaggatta actattcgta aaaaatatgg taaaggtcga ggtaaacctg ttatcggtta
     2941 tcgttttact tggaaagctg aaattaacca tgctgacgat ttttccaaag ggaaacaaga
     3001 agatttacgt ataaaattat ttaatattga tcacaatgga gaattaacac gagaagaaaa
     3061 atggcgcgct aaagatcgaa tactaaatct tcccttaggc actcatgaag ctgattttaa
     3121 acaacaacaa caaactgaag aaaaacaggc agaaaaacga gaactttcaa acaaactcaa
     3181 acaagattta ctaggaaatt tacaaaactt attcgattaa gcttaaggag tatttatggc
     3241 agataaaaca atcaaagaac ttgcggaaga attaggagtt agtaaaacag caattaacaa
     3301 aaaagtttct gacaaaaatc gaaaactttg gtttgcaaaa aacggaaaca agtttgtgat
     3361 taacgaaacc ggtcaaaaag ctataaaatc aatgttttta gtcgataacg caaacttaaa
     3421 tcgaaaacta gttggcgaaa aatcaaaaac caaaaaccat aaaaacacca accaagtttc
     3481 cgaattaaaa ctcctttctt tcctagaaaa acagttaaat cgaaaagata tacagctaca
     3541 agaaaaagat aatcaaataa aacaaatgca aaaattactc gaccagcaac aacaactcac
     3601 tctacaagcg aaccaacaaa ttgagaacct acaagaacag ctccatttaa cttatacaga
     3661 agaaactcca acaaccgaat caactgcctt ttctgaaaaa aaagcagaca atacacaacc
     3721 acttattgaa aaaaagtggt ggcaaatttg gaaatctaat agaaccaatc tctaaaattt
     3781 tctagaagtt agttcggata gtagaggaaa aagttgtact acctgatggg gagatcactc
     3841 aaatttttat gagaaagaaa aaatagttta caactgtaaa caatcaatgt ttatagttgt
     3901 aaactatcaa actatatggt gacttattgc ttaaaatagg ttgaaatctc actcaatttt
     3961 tggtggaaaa acaaaataaa ttctaatcga ttttgttgaa aaagtcataa aaaaacaaat
     4021 gtgattttaa caaaatttca aaaaaatcgt ttgaaatttc gtttattttt tttgagaatt
     4081 ttgatcatcg tagggtcatt gtagaatcat tatgagatca tcgtgggatc atcgaataaa
     4141 gtatgggaaa ggcttgcggt gcttggtatt aagggcattc tccgcctccc aggttcttga
     4201 ccattttctc tcaacgtggt aaaaatccca cttataagag aaaatggtca agaacctaaa
     4261 cggtcggcga agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DAHEYX010000194         4435 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHEYX010000194 DAHEYX010000000
VERSION     DAHEYX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.195"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            93..974
                     /locus_tag="MB219_002336"
     CDS             93..974
                     /locus_tag="MB219_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8428034.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            987..2597
                     /locus_tag="MB219_002337"
     CDS             987..2597
                     /locus_tag="MB219_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCC8428035.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2674..3333)
                     /locus_tag="MB219_002338"
     CDS             complement(2674..3333)
                     /locus_tag="MB219_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCC8428036.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3616..4281)
                     /locus_tag="MB219_002339"
     CDS             complement(3616..4281)
                     /locus_tag="MB219_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCC8428037.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1195 a          861 c          873 g         1506 t
ORIGIN      
        1 taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag
       61 tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt
      121 tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag
      181 gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac
      241 ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg
      301 aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg
      361 gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg
      421 ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa
      481 actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg
      541 atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc
      601 aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc
      661 tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg
      721 aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag
      781 aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc
      841 acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca
      901 aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac
      961 attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt
     1021 tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg
     1081 ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg
     1141 gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt
     1201 cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca
     1261 gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag
     1321 aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag
     1381 ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga
     1441 tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt gggctatctg
     1501 taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa
     1561 gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag
     1621 caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt
     1681 tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc
     1741 taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac accggctata
     1801 tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat
     1861 tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg
     1921 cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga
     1981 aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg
     2041 tcgtcttgat ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag
     2101 aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt
     2161 attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg
     2221 gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga
     2281 tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg
     2341 gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact
     2401 cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag
     2461 ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta
     2521 cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag
     2581 acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt
     2641 caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc
     2701 aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa
     2761 ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat
     2821 tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac
     2881 tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt
     2941 ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga
     3001 gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga
     3061 ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg
     3121 taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac
     3181 gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg
     3241 atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg
     3301 aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta aacagctatt
     3361 tctaataaag aaacataact tcccttattc caactgataa ttttctttta tcatagcata
     3421 aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat
     3481 tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt
     3541 tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg
     3601 cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag
     3661 ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa
     3721 tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt
     3781 tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg
     3841 ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa
     3901 ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa
     3961 atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg
     4021 agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct
     4081 ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg
     4141 aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc
     4201 tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata
     4261 atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga
     4321 attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa
     4381 cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attggctcta taatt
//
LOCUS       DAHEYX010000195         4355 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHEYX010000195 DAHEYX010000000
VERSION     DAHEYX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.196"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..330)
                     /locus_tag="MB219_002340"
     CDS             complement(<1..330)
                     /locus_tag="MB219_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428038.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADL"
     gene            complement(538..1464)
                     /gene="glsA"
                     /locus_tag="MB219_002341"
     CDS             complement(538..1464)
                     /gene="glsA"
                     /locus_tag="MB219_002341"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCC8428039.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1487..2914)
                     /locus_tag="MB219_002342"
     CDS             complement(1487..2914)
                     /locus_tag="MB219_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCC8428040.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2945..4159)
                     /locus_tag="MB219_002343"
     CDS             complement(2945..4159)
                     /locus_tag="MB219_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCC8428041.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1370 a          925 c          774 g         1286 t
ORIGIN      
        1 aagatcagcc agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa
       61 aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa
      121 tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc
      181 ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt
      241 ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa
      301 tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg
      361 attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct
      421 ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc
      481 tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta
      541 ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc
      601 gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac
      661 agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa
      721 ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact
      781 ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc
      841 agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc
      901 gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga
      961 gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt
     1021 aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc
     1081 tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg
     1141 atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc
     1201 cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac
     1261 gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag
     1321 ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa
     1381 tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa
     1441 ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat
     1501 gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag
     1561 aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa
     1621 caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt
     1681 ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca
     1741 gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg
     1801 acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc
     1861 agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt
     1921 cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag
     1981 gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa
     2041 ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa
     2101 tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc
     2161 caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct
     2221 ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta
     2281 aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt
     2341 tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa
     2401 acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag
     2461 tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg
     2521 ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac
     2581 cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga
     2641 ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt
     2701 tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata
     2761 gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat
     2821 attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt
     2881 tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt
     2941 tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt
     3001 ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat
     3061 aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc
     3121 ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga
     3181 atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac
     3241 gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt
     3301 ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta
     3361 gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca
     3421 tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc
     3481 gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag
     3541 cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa
     3601 gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag
     3661 cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag
     3721 ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc
     3781 tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa
     3841 gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat
     3901 gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg
     3961 ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt
     4021 gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac
     4081 caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat
     4141 tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg
     4201 tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa aagacagaca
     4261 attaataaaa gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa
     4321 ataaacggtc ttctttttat agaaaaccgt ttatt
//
LOCUS       DAHEYX010000196         4240 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHEYX010000196 DAHEYX010000000
VERSION     DAHEYX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.198"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            108..2048
                     /locus_tag="MB219_002344"
     CDS             108..2048
                     /locus_tag="MB219_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCC8428042.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2135..4057
                     /locus_tag="MB219_002345"
     CDS             2135..4057
                     /locus_tag="MB219_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCC8428043.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1428 a          717 c          880 g         1215 t
ORIGIN      
        1 gcgaaatttc tatttattcc aagaatttca gttattacgg aatcattttg tgcttaataa
       61 aaagaaaccg tttttatata ctataaatgt agaaaggcgg taggaaaatg aaaaaaatta
      121 ttgcaatcac atcctgtcca gttggtattg cccatacata catggcagca gaaaacttag
      181 agaaagcggg gaaagcagtt ggcgccgagg taaaagtcga aacacatggt tccatcggta
      241 ttgaaaatga attgactgca agagatatcg aagaagcagc tggtgtaatc atcgcagcag
      301 atactaaaat agataaatca cgattcggcg gtaaaccttt gataaccgta ggtgtgcaag
      361 aaggaatcca ccatgcagat gagttggtga gagatatttt agcaggaaaa gcgccagtct
      421 ataaaagtac ggaggcaatt atttcttccg aaaataaaag tgaatcagaa aatttaggta
      481 aaaaaatcta taagagtttg atgaatggcg tttcgtacat ggtacctttc gtagttacag
      541 gagggttatt gatcgcgatt tctttaacat taggaggaac agctacacct gaagggatca
      601 agataccaga aggaacgatt tgggcaacga tgaatagtat cggaagtatc gctatggggt
      661 tgatggttcc aattctatct gcatttattg cgcaaagtat tgcagatcgt ccaggacttg
      721 ttcctggatt tgttggtggg atgttagcgg caaacggagc attatatggt agtgatgcga
      781 atgcgggatt cttaggcggg atcatcactg gatttttagc aggattcatt gctttaggaa
      841 tcaagaaagt gcgagtaccg aaagctttac aatcgatcat gccaattatt gttatcccaa
      901 ttttaggatc attggcagtt ggttttgtgt ttatctatgt tattggagaa ccagtagctc
      961 ttctatttag ttcattgact cactttttag cagggatgca aggaggaagt gaaattgttc
     1021 tagctatgat cttaggagcg atgattgctt ttgatatggg ggggccgttc aataaagtag
     1081 cctttttgtt cggatctggc ttgattgcag aaggcaatta ttccattatg gggccaattg
     1141 ctgtagctat ctgtatccct ccattagcat tggggctggc atcattgatt ttaaaaacca
     1201 aatttacgaa aacagaacga gaagctggta aggcgtcctt agcaatggga ctgtttggga
     1261 ttacagaagg cgctattccg tttgcttcaa cagatccact acgggtgatt ccaagcattg
     1321 taatcggggc aatgacagga agtgtgatcg cgatgctttc aggagtgaca gatcatgtag
     1381 ctcatggcgg tccgatcgta gcggttttag gagcagtgga tcacgtagcc atgttttttg
     1441 tagcagtgat tgctggtatt gctgtaaccg tgctaatgtt aagaatcctt aaaccgacat
     1501 tacaacaaac tgcggttgct gcagcaaatg gtgtgacagt agcagatcta tcagagactg
     1561 aaacaagtga tgtttatacg aaaactgaaa cttttgattc tatcacggat attatgtcag
     1621 atgaagcaat cgttttaggt gaaacagcaa caacaaaaga cggagtaatc gaagatttag
     1681 tccaacgttt agaaaaaaca gcggcgatca cagataaaac cagatttaaa gaagtcatct
     1741 atgatagaga acatgaaagt acaacgggaa tcggtatggg gattgctatt ccacatggaa
     1801 aatcagctta tgttcttaaa ccaacagtgg catttgccag aagtcaatct ggggtagatt
     1861 ggcattcttt agatggaaaa cctgcacata tgatctttat gattgctgta cctgaaaaca
     1921 gccaaggtga tatgcacttg aaaatactgc aacgtctatc acgtaaattg atggatgatg
     1981 acttccgtca agcactgatg gaagcgccag ataaaacaac agttcaccaa ttattaagcg
     2041 aaatgtaaca taagatgaaa aatcgaccgt gatgagacat aatagatgtg ttatcatgat
     2101 cgatttttta tttagaaggg agtttctgca cattatgaat cagcgtcagc aatctttgat
     2161 ctatcaatta agtgagaaca ataattatat aacaacaaaa aagctagcag agcatttcca
     2221 tgtgtcagaa agaacgattc atagcgattt gaataccatt gaaacttggt tgagagacaa
     2281 acatctgcct ttaatgtttg aaagaaaaaa aggtacaggt atcttgttga atggtctgcc
     2341 tcacgaaagg gaacaactga aacgagcgct taatgaagag actcaaacag aaatggataa
     2401 aaaacagctt cgtcagttat tgattttcca tctgctagta gcaaaagacg gatttagtct
     2461 tgaagaactt tcagaaaaac tatatgttgg aaaacgtacg attcgtaaag aattgacaga
     2521 gttgcaaagc ttttttgagt atcaccattt acagctagta tccaaaacaa aactaggaac
     2581 ttttcttaaa ggcgatgaac aagaaaaaag acagttgctt gttaagacat tacgaaatat
     2641 gcagaaagaa gatccacaaa gtccgtcgtt gaaagaattc ttcgctaaag atacattaaa
     2701 agttatccaa catacattac aagatgtttt ttatgaaaac catttagaac agccgaatgg
     2761 actggctagt gtggagattc acatctactt tatgctcgag cgaatgaaac agtaccagaa
     2821 agtgaaatta agtaaagacg aaaacgaagt cgtggaacac acccaagcac aaaagttaag
     2881 tagtcaaata ttagcaaaac tagccaccat ctatccaatt gaattttctc ctgatgaaat
     2941 caattatctg gctttgcgta tcgccaatct ttttacaaat tcacaagcag ccacacgttt
     3001 tcaaaaggaa tctagcgcac ttgctgatca tttgatcgaa caagtggaac agtttctagg
     3061 ctattcatta aaagaagatc agctactaaa acaaaattta aggtctcatt tatcttcaac
     3121 ttattttcga ctccattatg gtttgcagat ttctaatcca ttgacgaaaa atgtgtttag
     3181 tacgtacaca caacttttct tagttttaca gttaatttta gaagattatt ttgaaaagga
     3241 aaatttttat gttccacaag aggagacagc ttatttaacg atccatttcc aagcagcgat
     3301 cgagcgacac aaacatcaga aaagtagaag atatcaaacg gttttagtta gtgaatattc
     3361 aaaagctatg gctacttttc tcgaagcacg gctgaacaga gaacttccag aattaacgat
     3421 tatggatcta gttgaatata agccagaatt aaagagacaa gattttccct ctgttgattt
     3481 tatcttggct acgttaccat ttaaacatat aagcattcca gtaatcgaga tctcaccaat
     3541 gatcacagaa actgatctag cctatttgac aaaatatatg ctggagcatg tgcctatcaa
     3601 gaagaaaaaa acatttgatc tagcaagctt tactcatcca tttctgatct ttcctcaact
     3661 tgaacggaca gacccagccg atattttaaa ctttatggga aacgtcttag tggaacatca
     3721 ttatgtggag ccggaattca tcgattctgt cttggaaaga gacagtcact ccagtactag
     3781 agtagcacca tttgtaacaa tccctcacgg taatccgctg tatgtgaagc attcgatgat
     3841 ttctattgct acaatgaaag aaccagtcct atggcacggg gaacagatac gtatcgtact
     3901 gctattagca ataaagaaaa atgatttgaa acatgcagag ttcaaaaaaa tattttctgt
     3961 tattcactat ctagaaaagc agccagaaca aatggctcag ttgatgcaat ccaatcatcc
     4021 gctagaaatc ttaacgttgt tatctagtta tgaataagta gcgattaaaa aactgccttt
     4081 atatttataa taattctatc tatttatttg gaagtagaaa actaaataca aaaaaagagg
     4141 atgtggccaa agttgtggca catcctttta ttttgagttt cttataattt tttatagttg
     4201 cttacgatgt cttgactact ggtaaacggt gttcaagaga
//
LOCUS       DAHEYX010000197         4170 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHEYX010000197 DAHEYX010000000
VERSION     DAHEYX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.199"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(88..1854)
                     /locus_tag="MB219_002346"
     CDS             complement(88..1854)
                     /locus_tag="MB219_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8428044.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1814..3568)
                     /locus_tag="MB219_002347"
     CDS             complement(1814..3568)
                     /locus_tag="MB219_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8428045.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3678..>4170)
                     /locus_tag="MB219_002348"
     CDS             complement(3678..>4170)
                     /locus_tag="MB219_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCC8428046.1"
                     /translation="SSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVL
                     LLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIP
                     IWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKK
                     YKK"
BASE COUNT         1400 a          793 c          584 g         1393 t
ORIGIN      
        1 ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat atcccaaaaa
       61 ttgtaggtta atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa
      121 tttctcagat agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa
      181 ctcactatga gtaccttgat caactatttt tccttgatcc atcactacaa ttttatttac
      241 cttttcaata gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg
      301 atttaaactt tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc
      361 taaaattaaa attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc
      421 tcctccagaa agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt
      481 ttcaataaag tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc
      541 ttgactattt ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg
      601 aaaaacaata gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc
      661 atcaattaaa atacagcctt catcataatc atacagtcta gacactaatt tcaagatact
      721 agttttcccg gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa
      781 agagatatga tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc
      841 aatgtcaaaa gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc
      901 tctaattcgc tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga
      961 atcgaaggaa tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt
     1021 cacttcaccc gttaacagta agtgaacacc tactagaatg actactgcta aactaacata
     1081 tgagaaaata gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat
     1141 cctttcactc tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa
     1201 gctattaatc tcttgctgca attctatagt ttcttgaaat tcttccgagt tttctctcaa
     1261 agtatcgtaa tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac
     1321 aaaactaaat aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag
     1381 catcactgaa ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact
     1441 catcgcatgc tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt
     1501 atgttttgaa aaataggata atggtaaatt tgacaaatcg tctgcaattt gcattcttaa
     1561 atgcgcactt tctttataag tactattata taagttttca tactccctat ctaacaacca
     1621 atacatgaaa agcaaagtaa cgaacgatac tgtaaaataa agccaaggcg gctttacatt
     1681 ttctaaaact aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca
     1741 gtatacaaaa aagctagaaa tagatgcttt aaccaaatct tctgcgccct gattagttaa
     1801 agcgaatttg tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat
     1861 aattagtgac caattgttta tacacttgat gtttagccat taattcatta tgatttccac
     1921 gttctactat tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata
     1981 atcgatgagc tatcataata actgtttttt ctttcatcag atgttgaaaa gccttttgta
     2041 gttcatactc attatctgga tcaatagatg cactggcttc atccataata acaatctttg
     2101 catcttttaa aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa
     2161 ctcctttact accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc
     2221 aaccggctaa tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag
     2281 aaacgttatc gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg
     2341 tgttaataat tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc
     2401 ccttatccac attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg
     2461 attttcctac taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa
     2521 cacactcttc tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct
     2581 gtgtctcgtt cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg
     2641 tttcgactgc atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag
     2701 aaacaaaaat aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg
     2761 gagtggtaag agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa
     2821 aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa
     2881 acttagcata atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa
     2941 atatctttat aacttgaatt cctcggatat attctaccgt ttcagagctt aacacatcta
     3001 acgattcttg atatttttga ataaaatttg tattctctgt cattttcttt aacaagaata
     3061 caccaattac taacatggcg attagaacca atccaatttt aaaattaatg aaaaagccta
     3121 aggcaactga taaaacaggt gttactatgg cttgagcact atctggaatc aagtgagcaa
     3181 ttgccatatg tgttttagcc gcgttgtcat caattgtctt tcgaatatat cccgaagaat
     3241 ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt tttctcaaat
     3301 tagtctctaa gcgaaaccca aggatatgag aaaataagcc agaaaaaata taaaataaag
     3361 ctcctaaagt taaccaagca acagtttgaa ccgcataact cttagctaaa tattcatctc
     3421 ctacaataat tagagactta agaaatttgt aaatactgta atatccatag actgttaata
     3481 cgacagaaaa atcagaaaat aaagtggcta accatccgta gtatttcatt ttagggacat
     3541 aactaaataa ttttttgtat actttcattt tttcctcccc cgcattcaat ttcttctctt
     3601 attcgtttac aactgttaat gctttccgta atagcgtaaa ttagagatgt acaaacagat
     3661 agaaggtaaa ctacgttcta ttttttgtat ttttttaaga cttttcttcc aatcaataca
     3721 ccaatcatag ataaacttgc tacaactaac actgaaatga ttccccatat acctaccgca
     3781 tagtggatca ttgcatccat tgttgcagca gattgtcctt tatctatcca atcttgacga
     3841 aaacgatctg taaagaacca tataggaatc cactgtccgg cagcactgcc taattgcatt
     3901 agagcccaag acgcaacttg aggaattgct cgatcatatc caaatacaga tagaataata
     3961 tcagctaaga tcgccatact aatcgaactg ataaaccaag gtaagtacgc cccgcccatt
     4021 aacgtaaaaa ataacaataa aacaattgtg taaatagtaa aaattccttt tctagggtag
     4081 ttatagctaa tataaacaaa tatgattcca gataataatc caaaaatccc tggggatatg
     4141 ctgtgaccaa acgccccacc aatactacta
//
LOCUS       DAHEYX010000198         4040 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHEYX010000198 DAHEYX010000000
VERSION     DAHEYX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.200"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(16..495)
                     /locus_tag="MB219_002349"
     CDS             complement(16..495)
                     /locus_tag="MB219_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCC8428047.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            854..1636
                     /gene="rpsB"
                     /locus_tag="MB219_002350"
     CDS             854..1636
                     /gene="rpsB"
                     /locus_tag="MB219_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCC8428048.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1735..2616
                     /locus_tag="MB219_002351"
     CDS             1735..2616
                     /locus_tag="MB219_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCC8428049.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2752..3474
                     /locus_tag="MB219_002352"
     CDS             2752..3474
                     /locus_tag="MB219_002352"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCC8428050.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3477..4034
                     /gene="frr"
                     /locus_tag="MB219_002353"
     CDS             3477..4034
                     /gene="frr"
                     /locus_tag="MB219_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCC8428051.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1437 a          717 c          880 g         1006 t
ORIGIN      
        1 gaatacgtgc tctgattaaa aaagtgatac ttcagttgga tggtgaagaa attcttctac
       61 tcgttttgcg tcctcctcat tttccgagat gaccatgatc gtatcgaatc cagcaattgt
      121 actgacaata gagggaggtt tgatctcatc taaaacagat gcaaataaat gagcattgtc
      181 tggcaaggta ttgacgatcg tcaaaaagtg gactcgttcg actttcaata caatgtcttc
      241 aatcatttga accagccgat cctcttcctc agtcctagtc tcaggagttc ctttttcgcg
      301 aaacaatacg aagcgggaaa cgccattttc atcaggtgcc ttcacgattt ttagatcacg
      361 gatatctcga gaaatcgttg cctgcgtggc ggtaacgcca taatgcccaa gtttgtttaa
      421 tagttcttct tgcgtacgga tcgggaattc ttcaatgact tgtttaatca gcaaatgtct
      481 ttgagctttg cgcattttct ttctccttta cgaaagattt cacatgtatc ataacaaaaa
      541 aaagaaggat tgcccaataa tattcgtctt ttatacctaa aattatttta tggaaaaagg
      601 ataataaaga aacaaagaga aatctactgt ttttcttgaa ttctttttat ctttcaggta
      661 aactattgaa tgttgagcag aaaagtatat ttgctcttgc gatacggatc ttttgtctgt
      721 ataatagctg agggtgcttt atgcatcagc aaatccacat tcttattgga ttgtcggtag
      781 gtgcttgaaa aagtttgccg gcaaggtaga agataagaag aggaaaaata aaaccaaatc
      841 ggaggaaaca aacatggcag taatttctat gaaacaatta ctagaagccg gcgtacactt
      901 tggtcaccaa actcgtcgct ggaatccaaa aatgaagaaa tatatcttca cagaaagaaa
      961 cggaatctac atcattgact tgcaaaaaac agttaaatta gtagatgcag cttatgatta
     1021 catgaagagc gttgctgaag aaggcggcgt agcattattc gtaggtacaa aaaaacaagc
     1081 acaagaagca atcaaagaag aagcaactcg tgcaggacaa tacttcgtta accatcgttg
     1141 gttaggcgga acattgacaa actgggatac tatccaaaaa cgtatcagcc gtttgaaaca
     1201 aatcaatgca atggaagaag atggaacttt tgaagttcta cctaaaaaag aagttgctgg
     1261 tttgaacaaa gaacgtgaac gtcttgaaaa attcttaggc ggtatcgctg atatgccaag
     1321 aatcccagat gtaatgtaca tcgttgaccc acgtaaagaa cgtattgctg ttcaagaagc
     1381 acacaaatta aatatcccaa tcgtagctat ggttgataca aactgcgatc cagatgaaat
     1441 cgacgtagta atcccttcaa acgacgatgc aattcgtgcc gttaaattga ttacttcaaa
     1501 aatggctgat gctttcatcg aaggaaacca aggtgaagat caagtagttg aagaagattt
     1561 cacagaagaa aacacagcaa catcaatcga agaaatcgta gatgttgttg aaggcgacaa
     1621 ttcttcagca gaataatttt gaatgatgga gctgtttcaa aggctgggcg acaagcctca
     1681 ctctcctttg aagcagcttt ttttaaaaag aaaaacacac aggaggagtt aaacatggca
     1741 gacgttacag ctaaaatggt aaaagaacta cgcgaaatga ctggcgtagg aatgatggat
     1801 gcgaaaaaag cattagttga agtagaaggc gacatggaaa aagcagttga tcttttacgt
     1861 gaaaaaggaa tggcaaaggc tgcgaagaaa aatgaccgca ttgcagctga aggattggct
     1921 tcagttgctg tgaaaggtaa cactgcagca atcgttgaag taaactcaga aacagacttt
     1981 gtttctaaaa acgaaatgtt ccaagattta gtaaaagaaa ttgcagaact tgttgctgaa
     2041 aacaaaccag cagacatgga agctgcaatg aaaatcaaaa cagataaagg tacaattgaa
     2101 tctgacttga tcgaagcaac tcaagttatc ggagaaaaaa tcagcttccg tcgctttgaa
     2161 gttgtagaaa aagacgacaa tgctgcattt ggtggatact tgcatatggg cggacgtatt
     2221 gccgtattga ctgttctaga tggaacaact gacgaaacag ttgcaagaga cgtagctatg
     2281 cacgtagcag ctatcaatcc tcgttatgta aacgaatcac aaattccaga agctgaatta
     2341 gaacatgaaa aaactgttct tacagaacaa gcattgaacg aaggcaaacc agcgaatatc
     2401 gttgaaaaaa tggtagaagg acgcttgaag aaattcaaag cagaaatcgc tttggtggac
     2461 caaccattcg ttaaagatcc tgatatgact gttgaaaaat acgttgcatc aaaaggcgca
     2521 actgttaaaa cattcgtacg tttcgaagtt ggcgaaggta tcgaaaaacg cgaagataac
     2581 tttgtagaag aagttatgag ccaagtgaaa aaataattct gttgaataac agacgggaac
     2641 gcacggtttg tgcgctccct ttttttaagc aaaacaagaa aaaccagtct tagagtgtcg
     2701 aaatcatcta agtatggtaa aatggcaata gaaaatattg gaggaatgaa catggtcaca
     2761 ccgaaatatc agcgtgtagt cttaaaattg agtggagaag ctttagctgg ggacgcagga
     2821 tttggaatca aaccaccagt aatcaaagaa atcatccaag aaatcaaaga agttcacgag
     2881 ctgggcgttg aaatggcaat cgttgtgggc ggcggaaata tctggcgcgg acaaatcgga
     2941 gcacaaatgg gcatggaacg tgctcaagct gattacatgg gtatgttagc tacagttatg
     3001 aatgccttag ctttacagga tacgttggaa aacgtgggtg taccgacacg cgtgcaaact
     3061 tcgattgaaa tgcgtcaaat cgcagagcca tatattcgcc gtaaagcaga acgtcattta
     3121 gaaaaaggac ggatcgttat ctttgcaggc ggtaccggca acccgtactt ctcgacagac
     3181 acaacagcag cattacgcgc ggcagaaatc ggcgcagatg ttatcctgat ggctaaaaac
     3241 aatgtagacg gtgtttattc tgctgatccg aaagtagatg caaatgcagt aaaatttgaa
     3301 gaactgactc atctggaagt tattgccaaa ggattacaag tcatggattc aactgcaagt
     3361 tcactaagca tggataatga tattccactg cttgtcttca atttaaatga acacggaaat
     3421 attcgccgtg caatcttagg cgaaaatatc ggaacaactg taagggggaa ataaaaatgg
     3481 cagatgcaat tatgacagaa gcaaaagaaa aaatgaaaaa agcagcacaa aatcttcaac
     3541 gtgaacttgg gcaaatccgc gcaggccgtg cgaacgctag ccttttggac cgtattactg
     3601 tgaactacta cggtgcgcca acaccattga accaaatggc atcaatccaa attccagaag
     3661 cacgtgtttt aatgatcaca ccttttgaca agagcattct tcaagatgtt gaaaaagcta
     3721 ttatggcaag tgatatcgga atcagtccaa ccaatgatgg aaatgtcatt cgcttagtta
     3781 tcccgcaact gacagaagaa cgcagaaaag aattagctaa agatgtgaaa aaagaagcag
     3841 aaaatgcaaa aatcgcgatt cgtaacatcc gtcgtgatgc gatcgatgaa tataaaaaac
     3901 aacaaaaaaa tggtgatatt accgaagatg atttgcgcgg attagaaaaa gacgtacaaa
     3961 cattgacaga taacagcatc aaagaagtag ataagatcgc tgctgataaa gaaaaagaat
     4021 tattggaagt ttaataaaat
//
LOCUS       DAHEYX010000199         3909 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHEYX010000199 DAHEYX010000000
VERSION     DAHEYX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.201"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..382)
                     /locus_tag="MB219_002354"
     CDS             complement(<1..382)
                     /locus_tag="MB219_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428052.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYF"
     gene            649..2349
                     /locus_tag="MB219_002355"
     CDS             649..2349
                     /locus_tag="MB219_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCC8428053.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2319..2537
                     /locus_tag="MB219_002356"
     CDS             2319..2537
                     /locus_tag="MB219_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCC8428054.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2557..3447
                     /locus_tag="MB219_002357"
     CDS             2557..3447
                     /locus_tag="MB219_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCC8428055.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3465..3749
                     /locus_tag="MB219_002358"
     CDS             3465..3749
                     /locus_tag="MB219_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428056.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1368 a          646 c          797 g         1098 t
ORIGIN      
        1 cgaagtatac aaaattattc tgtagtttat aaaaaaacgg cgaatgaaga tgtgttttca
       61 tatgaccaaa atataataat tctgaaattt ctaaaggtgt caattctttc agcatcgtta
      121 aatcagtaaa gtcgatccac ttcaattcat cgaatccacg cctgttacga gaagaggaaa
      181 agaaacgctg aatattttct gtaccacgga ttactttcag ccctgtatgg gattcatatt
      241 ctccagcaga agcagatgga tcaagcaaga gcaagttttg aggaaaatgc acgatactgt
      301 ttgcaaagtc agtagcagtc agcccttttg ttaaaacagc attacttgtg gtatcaatat
      361 gtacataaac tgactcagtc atcatgttcc tcctcttatt tcctcttaat ctcttttatt
      421 ttacaacatt tgcattaaaa aagcttgttt cttttatttt aaaattccat tacaaaatca
      481 cgttttcatt gaaaacactc tcattttcaa ttaatttttg tgatttttgt atttttttat
      541 ttaaaggagg ttttatgaat tgttttctta tggaatctac aaagtcgaat acgagactca
      601 tgaccttttt atgacgtact ggtatacttg aagaaaaagg agagcgcgat ggagataaaa
      661 aaagtttact tttcacagaa aatgtcgctg tattcgccta tccagcaagc aaaacaaccc
      721 cttccatttt ttcaaagtca tcaagaaagt agagacgctt tttggcgttc tgcaaaaaag
      781 caatgtgctt ttaaagcagt atcgaaaaaa tataccgtct attttactat tgctaaaagt
      841 agaggagatg ttatttttaa taatctgttg attgaaggtg aaacttttga atggaaaaaa
      901 ttttttcggt atatggaagc ttgcgctcgt ttttttatta aagaaaactg ttgtttttta
      961 tttcaatctc ctctatcaga agaatggttg cgtatttttc ataaaaacgg ctatcaagga
     1021 accacacaat taaataaaaa gctggtctat catacagcac ttgttttagg tggaggagga
     1081 gcgcacggtg cttatcagat tggtgtgtgg caggcgctga aggaacatga tatcaacttt
     1141 gagattatca caggtacctc ggtaggtgcg ttgaacggcg cattgatttt acaaggagat
     1201 atgaaaaaag ctgtgaattt atggaaaaag ctgtcgacaa gacaagtgct tgcgttacca
     1261 gaaatggctg cagttgaaga ccttcgtgaa cgcttctatc gagaaagacg ccaaatgacg
     1321 aaaacagcac taattgaagg cggggtttct tctgctccat tggaaaagat ggtcaaagac
     1381 aatttggatt tgtccttact caaacataat cccaaaatac gtttatatac ggtgtctact
     1441 aaactgccag aactagcgga agtagtgact gatatccaac agacaaaaac agaagaaata
     1501 ccagattgga tactcgcctc tgcttcattt tatcccgcta tggcttatcg aaagatcgga
     1561 aaaaataaat atgcagacgg tgggtatcgc aacaaaatac ctattgatat tgcgattaac
     1621 aaaggagcaa cagaggcatt tgtcgtagat gtgcaagggc caggcccagc gaaaaaaatt
     1681 cgtatgccag acatttttat ccattggaaa tgtcaaacat tgtggacact aggcagcttt
     1741 ctgctttttg atagccaaag gaaccaatta aatttacaat taggctatct ggaaatgaaa
     1801 aaacgattag gctattacta tgggaactgg tatacatttc attctgttaa acctgcaaga
     1861 acatactggc gaggattttt gtcttactta acaaaagaaa ttcagttaga attatctttt
     1921 ttcaaaagca taaaattttg gcaaaaactg cgtaatttat ataagaacag ggtcgtacca
     1981 gagacatgcg gattggcgat gctagaactt ttagcaaaaa aacatttttt actgcccaat
     2041 gaaatctatc aagtagacat gatgattcaa atgatctgcc aacaagatat aaaaagtatg
     2101 ccggatgatt tattggcaca aattggtcaa ttaagtactg aagaatggcg aaggtatcga
     2161 aaataccagc aaaaaataca aaatgagcgc acgaaaactg tttattttga ttatctgcta
     2221 caaggaaaac gcaaggatcg tttgcagcat gaattgctta agcaaccagt tgacacatta
     2281 ctgatcctat atttatatta tctcaaggag gaacaaccat ggcacaagaa ttttcatatg
     2341 aaatcgtaga agaaatcgct atattgtcag aaaacaacaa ggggtggcgt aaagaactga
     2401 acctagtcag ctggaatggt cgcccgccaa aattcgatct tcgagattgg gcgccagacc
     2461 atgaaaaaat ggggaaaggc ctgacattga cgaacgaaga gtttgaacaa ctgcaaaaag
     2521 caatagaaaa tatgtaatga ggaaggaccc tgtcgaatga aaagtatgac tggttttggc
     2581 aaagccatcc gcgaaacaaa agcgtatcag ttagatattg aaataaaaag tgtcaaccag
     2641 cgttttctag atgttcaaat acgaagtcct aaaatcctga actttgttga aaacgatttt
     2701 cgtcagctta ttaaacagca gttaagccgc ggtcgaatcg aagtatttgt gaatctagct
     2761 tatattggca aaaacgaaaa acagatcgtc atcaactggg aattgattga ttcacttatc
     2821 actcaattga cagacggaat tcaacaacga tacggtagtc aaaatcaagt gtcaattagt
     2881 cggatggcgg aacgcttcgc gctggacgaa aacttcgtaa cggtagaaga aaaaccggaa
     2941 gagagcatgg aagaattggc acagttagct ctagatacat tgaaaagcgc acttgattct
     3001 attgaggcga gtcgagaaaa agaaggtcaa gctttagctt tagtgttgaa aaagaatact
     3061 gaagaattca aagaagtact aaaaaaactc gatagttttg tagaaatcta tgaaaaggaa
     3121 tttcaaataa ggtatcaaaa aaagctggag gaatttcttg gagcgaaagt cgatgagcaa
     3181 cggttgttga cggagctagc catcttgttg gaacgcggag atattcacga ggaactggat
     3241 cgtctggcga tccatgtaca aaaactagat gaacttatcg aagcacaatg tcctgtaggt
     3301 cgggaattag atttcctgat ccaagaaatg aatcgggaaa tcaacacgat cggttccaaa
     3361 tcaaatgcaa tcgaaattaa aaatcaagtc gtccagtcta agacgatctt agaaaaaatc
     3421 agagaacaag tacaaaatat tgaatgacga aagaggtaga gataatgaaa tattatgacg
     3481 taacgttcca cgaattaagt ggaaaaactg tggttaaacg ggatatccct tctgaaaaaa
     3541 acggctttga cgtatggaaa gatgcctgtg cagactataa tgagaacgaa ctgtttattt
     3601 tgatcaacga cggtgcttac gtgacgatga atcggaaatt tattgttcga attgacacag
     3661 aagaggtaga agatccgaca gaaaaagcac gttctcgaaa agatgagatc atgggtgtag
     3721 tgaatacttt gtccaatatg ggcttttagc aaggatgctg aacaaagaaa tacaataaat
     3781 aatcagaatt ggatttcttc gcttagcttc tttgctctag gaatacattt aaaggttgtg
     3841 acaaaattcg tgtcacaatc tttttttatt ttgaataagt gatgttcaaa aaccattttc
     3901 tcggtatta
//
LOCUS       DAHEYX010000200         3819 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHEYX010000200 DAHEYX010000000
VERSION     DAHEYX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.202"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            326..943
                     /locus_tag="MB219_002359"
     CDS             326..943
                     /locus_tag="MB219_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCC8428057.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            984..1283
                     /locus_tag="MB219_002360"
     CDS             984..1283
                     /locus_tag="MB219_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCC8428058.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1317..1484
                     /locus_tag="MB219_002361"
     CDS             1317..1484
                     /locus_tag="MB219_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCC8428059.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1529..2092)
                     /locus_tag="MB219_002362"
     CDS             complement(1529..2092)
                     /locus_tag="MB219_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCC8428060.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(2120..2800)
                     /locus_tag="MB219_002363"
     CDS             complement(2120..2800)
                     /locus_tag="MB219_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HCC8428061.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
     gene            2875..2964
                     /locus_tag="MB219_002364"
                     /pseudo
     CDS             2875..2964
                     /locus_tag="MB219_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3041..3370
                     /locus_tag="MB219_002365"
     CDS             3041..3370
                     /locus_tag="MB219_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCC8428062.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3360..3647
                     /locus_tag="MB219_002366"
     CDS             3360..3647
                     /locus_tag="MB219_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCC8428063.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT         1259 a          617 c          717 g         1226 t
ORIGIN      
        1 atagtgttaa aaaaattgta aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata
       61 taccatggaa gacataaaag aaaaactgga taactagttt atttaatggc agaccgttaa
      121 ggcctgccat ttttatgtat tttagcggaa taaatataat catgcgggag tgtaaactta
      181 ctgtttcaaa aagtatactt tttgacatgc cttgattttc tttgtttatg tatggcataa
      241 taaccacata atacaatttg aaacgtttct gatttaatct gattgttttg aaacaatttt
      301 agtgaaaaaa gggtagataa aaacgatgag aaaatttttt tatatccggg tcagtagtta
      361 tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa ttaggaatcc ctgaagaata
      421 tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc cctgaatatc aattaatgaa
      481 acggatgttt cgaaaaggag atttactcta tctacaatca ttagatagac taggcagaaa
      541 taaacaaatg attttagaag aatggcgaga attaactaag gaaaagcaga ttgatatttc
      601 tgtgttagac atgcctctgt tagatactgc tagttacaag caactagatg ggttagagac
      661 gcttatctct gacttaattc tgcaattact cagctatatg gccgaagacg agcggaaacg
      721 aattaatagc cggcaaagag aaggcgtaga agctgcgaaa caaaagggtg taaaattcgg
      781 cagaaagaaa attgctttac ctcctgaatt tcctgcgatc tattctgact ggaaagcagg
      841 aaagataact gcagtctatg ctatggatca attgggcatg aaacgaaata ccttttatcg
      901 acgggtgaaa gaatatgaac ataccttgca ccacttttct tgatagatat aacagctatt
      961 agacgggaag ggagagtttg aaaatggact taacgaatca aagggtcgtt tttactggaa
     1021 cgctgcagca aatgacacga acccaagcca tcggtttggt tcactcttta aacggccatt
     1081 gtcaatcaag tgttacttca aaaacaaatt tcctggtttg tggtcagtat tctaagtcgt
     1141 tgtttgatga ttctctctat acaaaaaagg aacaaaccgc gcgggacttg attgcgttag
     1201 gtcatgagat taccatttta tcagaagtcg aattttatgc gttaattagt caacagttga
     1261 aagaatggca acggtattta taatcgttag taaaatttac aaggaggaaa atttttatga
     1321 agcgaaaatc ttatgatcgt tggggcattg aaataactag ttttaatcga aatattgctt
     1381 ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt ttatcaaaaa aattactttt
     1441 actgtcctaa atgccatcgc cgatacgggg tacatggaac atagaaacga aatagtgcgg
     1501 tagaaaagcg aatgaatgta gtatatttct acaaatcatt cgctttcttt attttataaa
     1561 tagttggtcg agagatcccg ttttcttttg caatctctgc aactggaatg ccttgcttta
     1621 acatgtccac cacggtttta aaaactaagc gtttttgcgg atctgttgag ttaatagaat
     1681 actcgattgg tcgtccctta tatcggccgg ctttttgagc caataatact ccttgccgct
     1741 gacgttcacg aattttctca cgctcgtttt gtgcaatata acttagcagt gataaaaaca
     1801 tatcgaacat agcggtatcc aggagttcat ttccagtatt aaaatctaaa aactgtgcat
     1861 ctaatatttt taacgctact tttttctgtt tcataaaagc aactgtattt tttatatctt
     1921 cataatctct tcctaaacga tctagtgatg taacgatgac gcaatcacct tcacggatat
     1981 aatttagtaa tttttgaaac tcaggacgct cagtatcttt accactggct ttttcttgga
     2041 ataatttatc aatgttaagt ccttcggttc tgttgcgaag tttgaaaatc aaacgcgccc
     2101 tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt tcagtagaca
     2161 ccgaaaagcc gaagagcgtt ccatttcttc ggttcttttt atatattcct cgaatggtct
     2221 ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta tttcgtcgtt
     2281 taataggtcg atggtcttgt tctattaaat tgttaagata cttcacagtt cggtgctctg
     2341 tcttagtata taaacccaca ctctgtaact ttctaaaggc ggagccaaga gaaggtgctt
     2401 tatcggtcac aattgctttc ggctcaccaa actgtttatg gagtcgtttt aagaaagcat
     2461 aggctgcttg cgtatcccgt ttctttcgta accagatatc taaggttaag ccgtccgcat
     2521 caattgcacg ataaagataa tgccaacgtc ccttaatttt gatataggtt tcgtccattt
     2581 tccatgaata gaaggattgt ctatttttct tcttccaaag ataatagagg actttgctgt
     2641 actcttgcac ccaacgataa atcgtagtat gacaaacatt tattccacga tcatataaca
     2701 attcctgaac ttcacgatag cttagattgt aacgcaggta gtaaccaaca gcgacaataa
     2761 tgacgtcttt tttgaattgt ttgcctttaa aatgattcat tactctgtcc tctctgtctt
     2821 ttttctcaat tttacactaa aatagatttt ttggaaaact ttgcaacaga accgaataga
     2881 caacaccggg ttgtttataa ggtagatgaa gatgaacacg tggttgaaat ttattcagca
     2941 aggacgcatt acgagtcaca gtaattactt gtaaatacat tatctcgtgg tataatgtat
     3001 ttactaagta attacatact gttagaaatg agggatttaa atgattacaa aaacaagaaa
     3061 acaaggcaat tcaatcatgt tgacagtacc taaagatttt aatgttccta atggagtcga
     3121 agttgaagca aagttggtag agaatggtat tctttatgaa tttgttgaac cacaaaaaga
     3181 attttatgat tttagcgaag atattttgtc tgatataatt gcagaaggct acgataaaga
     3241 tgagattctt gtagaattta agaatagaaa aaacaaaatg cattcttcct ttagagatat
     3301 tgctgaagat acacttacta attctaaagt aatgactaaa gaggagttgg caacagaaat
     3361 tggcttatga aatcgttcct tcaaaacatg tcattaagta tttaaaaaag ctaaaagaga
     3421 agccattgaa agaaaaattt ttgaatttaa tttatgatga aattgcgatt gatccatata
     3481 agggtgaaca aaaaactggg gatttatcag gtatttggtc tagtgggttc aaatatgcgg
     3541 gcacaacata tagagtagct tacgagatta tagaaaacaa ggttattcca gtattgttat
     3601 gtggaacgca tgaaaacttt tatgaacagt tgaaaaaatt aatctagtga tttgttgtcg
     3661 ttcttaagaa ggacggcttc ttttttatcg aaaatctgat tcgtctacat gactaaagtc
     3721 gcgagtattc tctgcttctc gataaattaa ataacgtcat tactttgagg aaagctataa
     3781 atcaatatat ttcaactttt ccttatttat gtactacct
//
LOCUS       DAHEYX010000201         3816 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHEYX010000201 DAHEYX010000000
VERSION     DAHEYX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.203"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..944
                     /gene="bsh"
                     /locus_tag="MB219_002367"
     CDS             <1..944
                     /gene="bsh"
                     /locus_tag="MB219_002367"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCC8428064.1"
                     /translation="HYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKNLDKHYAMIGIA
                     AGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFIPWILGQCSTID
                     EAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNN
                     PPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATF
                     TKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSCCNVDKGIYYYR
                     TYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            1269..3164
                     /gene="asnB"
                     /locus_tag="MB219_002368"
     CDS             1269..3164
                     /gene="asnB"
                     /locus_tag="MB219_002368"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCC8428065.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3359..3805)
                     /locus_tag="MB219_002369"
     CDS             complement(3359..3805)
                     /locus_tag="MB219_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCC8428066.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         1265 a          629 c          722 g         1200 t
ORIGIN      
        1 accattattt tggaaggaat tttgattatg agatgtctta taatgaagtg gtcactatta
       61 ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa tttagataaa cattatgcaa
      121 tgataggaat agcagctggg gtatccaact atccactcta ttatgaggct acaaatgaaa
      181 aagggttgag tatggcagga ctgaattttc ctggcaatgc tgattataaa gaattacaag
      241 aaggaaaaga taacgttgca ccatttgagt ttattccatg gatactaggg caatgctcaa
      301 ccatagacga agctaaagaa ttattggcta ctatcaatct agtcaatatc gattttagtg
      361 aaaaactacc cttgtcacct ttacactggt tattagctga taaagaaaaa tcgatagtaa
      421 ttgaaagtat gaaagatgga cttcatttat atgataatcc tgtcggtgtg ttgacaaata
      481 atccgccatt tgattatcaa ttatttaatc tgaacaatta tcgctcgcta tcaaatggaa
      541 caccagaaaa tcatttttca aaccagatca gcttgaatgt ttatagtcgt gggatgggag
      601 gattgggctt acctggagat ctctcttctg tttctcgatt tgtaaaagcc acatttacta
      661 aaatgaatgc tgcatcaggt gattcagaat ctgaaagtat cagtcaattt tttcatatac
      721 taggctcagt cgagcaacaa aaaggagtat gcgatactgg agaaagaaaa tacgaatata
      781 cgatttattc ttcttgctgt aacgttgata aagggattta ttattatcgt acgtatgaag
      841 acagtcaaat tactgctgta aatatgaata aagaagactt ggatcgtcat gaattggtca
      901 gttatccagt cataaaagag caacagataa actatgtcaa ttaattaatg aatagatccg
      961 tttggtatca tattttctga tacagtcata attagacacg atctttaggt cgtgtctttt
     1021 tttgctggtt tttttggaaa tagcatccac taatattttt ttaacacgaa gagaataagg
     1081 tagcctcaaa aaagaaaaca tggtaaatta gatattggga ctttattttc tgtaaaaaca
     1141 gaaactttca tcttttgttt tcacgtgacg aaattgtaag agagaaaata ttcgtccgct
     1201 atttattcta tgataagatg catatgaatg aaataaacag taattttata ttaggatgtg
     1261 aaagttaaat gtgcggtatt gtaggttttg ttaacgacaa ggacaacaaa aaaacaatta
     1321 ttaatacaat gatggatcga atcgttcacc gtggacctaa tagttcagga gaatatatcg
     1381 ataaacatgt tgctttagga ttcagaagat taagtattat tgacttagag ggcggtactc
     1441 agcctattta caatgaagat cggacaaaga tcattatttt taatggcgaa atctataatt
     1501 accagccttt gagagaagaa ttgattgcag ctggccacgt ttttcaaaca catgcagata
     1561 cagaagtcct tttgcatggt tacgaagaat ggggaacaga attattgcaa aagatccgcg
     1621 gaatgtttgc ttttgctatt tgggataatg aaaagaatga gttattcggt gcaagagacc
     1681 actttgggat caaaccatat tattatgcag aaatgaacgg aacatttatg tttggttcag
     1741 aaatcaaaag ttttctgcct catcctgatt ttaacaaaga attgaacaaa gaagcgttga
     1801 aaccttatat gactttccaa tattctccat taaacggcga aacattcttc aaagatgtct
     1861 atcgtttgcc tgaaggccat tattatacgt ataaagatgg aaaaatagac attcagcaat
     1921 attgggatgc agattttgaa acaaaagaaa cgcattcccg acaagaatgg atcgaaaaaa
     1981 tcgacgaaac cgttcaagct tctattgaag cacatacggt cagtgatgta gaagttggtt
     2041 ctttcttatc cagcggggtt gattccagtt atgtaacctc tgtattgaaa ccagatcatt
     2101 ctttctcaat cgggtttgat gataaaacct ataatgaagc gattgaagcc agaaaactaa
     2161 ccgaactatt ggacttagat aatacagcag cagtgatcga cggagacatg tcgttcaaag
     2221 cattcccttt gatccagtat cacttagatg aacctgattc aaatccttct tgcgtacctt
     2281 tgtacttttt agcaaatctt gcttcacaaa gtgtgcgggt agtccaatct ggagaagggg
     2341 cggatgaact gtttgcggga taccaaacgt atggattcca tacgaattca aaattcatcc
     2401 gtgtgattgc tcaaggattg aaaaaattac caaaaggaac acgctataat ttaggacgta
     2461 aaatcggaaa aatgaaaaat ttccatggac ggatccattt atatgaatca cttgctcctg
     2521 caaaagaata ttttatcggt catgctcgag tgttcgaaga atcagaagca tcagaagtgt
     2581 taacaccaaa atatcaaaca gcaccatctg tggatgaaat catgactgtt cattacgaga
     2641 aaacagaagg aatcaaagat gaagtcaata agatgcaata tgtcgatctt catcaatgga
     2701 tgccaaaaga tatcttgtta aaggcagata aactttctat ggcaagttct ttggaagtcc
     2761 gagtaccttt attggatatc gaagtcatga aattggctca acaaatacca agtaagtttt
     2821 tattgaatca aaacaacaca aaagatattt tcagacaagc tgcgaacaaa catcttccgg
     2881 aagaatggtc gaatcgtgtg aaattaggat tccctgtgcc aatcaaagct tggttgaaag
     2941 aagaacacgg ctacgaacaa gtcaaagcat tattcgaagc agattttgca aaagaatttt
     3001 ttgatcaaga aaaaatcatg aagttgctag atgaccatca cgaaggacgt aaagaagagc
     3061 aaagaaaaat ctggacaatc ttcagtttct tgacatggta taaagtctac tttgtagatg
     3121 aaacgattcc gcaagcagaa gcaatcgact atgtgactgt ttgatgaaaa tcaaatatca
     3181 ataaaaaaga ggttttctcc taccgtttaa cactgagtga taagaaacaa tttcaaaaaa
     3241 cagctcttcc cgttttatag atattatgac ttatggttcg gagttggcag aggaacacca
     3301 ttgtttcgaa cataaggggg ttgtgacacg attttcgtca caaccccttt acatatgttc
     3361 aataatcgat ttctttttct tttgtcccat cttttttata aatgaacaaa gttgattctt
     3421 tgttttgtgc tacttgcttt cctttttcga ttgcttcttt ttttgtatca aaatggttgc
     3481 ttgccttttt tgccccttta gatttcacga tccattgttc ttcctcatat tctacgatca
     3541 catctgaatc aagcagtttt ccagcacgag gattcgtatc gtgttcatcc gtcttgcttg
     3601 gatttttttc tttttcgaaa gttcgttttt ctgattcact agcatcttca taccattttt
     3661 ttgcttgatc aatggcaatt gggatcgcac ggttatcagg gtacccttca tctaataatg
     3721 catttgcgat gtcgattgct ttttttcttg tcaatttatc taagtttttc attgatgcag
     3781 gaaagtcctt catgttccat ggcatattag tatccc
//
LOCUS       DAHEYX010000202         3785 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHEYX010000202 DAHEYX010000000
VERSION     DAHEYX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.204"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..171
                     /locus_tag="MB219_002370"
     CDS             <1..171
                     /locus_tag="MB219_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCC8428067.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="MB219_002371"
     CDS             406..642
                     /locus_tag="MB219_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428068.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="MB219_002372"
     CDS             649..2601
                     /locus_tag="MB219_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCC8428069.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MB219_002373"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="MB219_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCC8428070.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="MB219_002374"
     CDS             3400..>3785
                     /locus_tag="MB219_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCC8428071.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAHEYX010000203         3759 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHEYX010000203 DAHEYX010000000
VERSION     DAHEYX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.205"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            183..497
                     /locus_tag="MB219_002375"
     CDS             183..497
                     /locus_tag="MB219_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCC8428072.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            513..899
                     /locus_tag="MB219_002376"
     CDS             513..899
                     /locus_tag="MB219_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCC8428073.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            928..2313
                     /locus_tag="MB219_002377"
     CDS             928..2313
                     /locus_tag="MB219_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCC8428074.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2316..2469
                     /locus_tag="MB219_002378"
                     /pseudo
     CDS             2316..2469
                     /locus_tag="MB219_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2492..3697
                     /locus_tag="MB219_002379"
     CDS             2492..3697
                     /locus_tag="MB219_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCC8428075.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1238 a          685 c          817 g         1019 t
ORIGIN      
        1 caataaaatt tgaatattta ataggtagac acttcaaaaa atgaggtgtc tattttttta
       61 cccgattttg aaaggaagtg aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc
      121 cccactcttt aagaccatca aacattcatt cagccaataa aaaagaaagg ataggtaaaa
      181 atatggaact taaatttgtg attcccaaca tggaaaaaac attcggcaat ttagaatttg
      241 ctggcgagga taaagtcgtt cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa
      301 gctataatct ctattctgat gttcaaagag cagatgatat tgtggtggtg cttcctgctg
      361 aagctggcga aaaacatttc ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta
      421 ccgcagaggg ctacaaaatc ggcactcgtg gttttacaaa ttacctttta catgctgacg
      481 acatgataaa agaataaaga aagagaggaa aaatgatgag attagcaaat ggcattgtat
      541 tagataaaga cacgactttt ggagaattga aattctctgc tctacgtcgt gaagtgagaa
      601 tccaaaatga agacgggtcg gtttcagatg aaatcaagga acgtacctat gacttaaaat
      661 ccaaaggaca aggacgcatg attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt
      721 ttgattataa cgcacgggtg gaacttatca atcccattgc ggacaccgtt gctactgcca
      781 cctatcaagg agcagatgtt gactggtata tcaaggcaga cgatattgtg ctgacaaagg
      841 attctagttc attcaaagct caaccacaag caaagaaaga accgacacaa gacaaatagt
      901 cgctaggtag aaaggagact ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct
      961 ggtaaagatt tagtctttca ttttacgata gcgtcactcc tgcctgtttt cctgctggtt
     1021 gtcggactgt ttcatgtgaa gacaatccag cagatcaact ggcaggattt taacctatca
     1081 caagcagata agattgacat tccctattta attatcagtt tcagtgtcgc aattcttatc
     1141 tgcttgctgg tagcgtttgt attcaaacgg gttcgctatg atacggttaa acaactttac
     1201 caccgtcaaa aactggcaaa gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc
     1261 aaaacagagg gtttctttaa agatagtgct ggtcgtacaa aggaaaagat aacctacttc
     1321 cctaaaatgt attatcgact taaaaatggc ttgatacaga tacgggtgga aatcacgctg
     1381 ggaaaatatc aagaccaact cttacacttg gaaaagaaat tagagagtgg cttgtactgt
     1441 gagctgacgg ataaagagtt aaaggattcc tatgtggaat atactttgct ctatgacacc
     1501 atagccagtc gtatttctat tgatgaagta gaagctaaag atggtaaact tcgcttaatg
     1561 aaaaacgtat ggtgggaata tgataagctc cctcatatgt tgattgctgg tggtacaggt
     1621 ggcggtaaaa cttactttat actgacactg attgaagcct tgcttcatac agattcaaaa
     1681 ctgtatattc ttgacccgaa aaatgctgac cttgcggact taggttctgt gatggcaaat
     1741 gtctactata gaaaagaaga cttgctttct tgcattgaaa cattctatga agaaatgatg
     1801 aaacgtagtg aggaaatgaa gcagatgaag aactataaga ctggcaaaaa ttatgcttac
     1861 ttaggtctcc cggcacactt cttaatcttt gatgaatacg tcgctttcat ggaaatgctg
     1921 ggaacaaaag aaaacaccgc agttatgaat aagctgaaac agattgtcat gttaggtcgt
     1981 caagctggct tctttctaat actggcttgt caacgtccag acgcaaaata tttaggcgac
     2041 ggaatccgtg atcagtttaa tttcagagtg gctttaggtc gtatgtctga aatgggctat
     2101 ggcatgatgt ttggcagtga cgtacaaaag gatttcttct taaagcgaat caaaggtcgt
     2161 ggctatgttg atgtaggaac aagtgtcata tcagagtttt atactcccct tgtaccaaaa
     2221 ggatatgatt tcttggagga aattaaaaag ttatccaaca gcagacagtc cacgcaggcg
     2281 acgtgcgaag cggaagtcgc aggtgtggac tgatcttgct ggctggtgtg gcaatagcca
     2341 cgccagcact taacccctcg gtatctaaca ggggggtaca aatcgacagg aaacagtcaa
     2401 aaaaacatta gaaaatcctt tggttacaag ggatttacaa aatttcagcg tatgtcaaat
     2461 gggctttaaa agttgacata cgcctttttg attggaggga tttttactga atgaacaaac
     2521 ttggttacag catttaaaag aaaaacgctt ggcttatgga ctatctcaaa accgtttagc
     2581 tgttgcgact ggtattacaa ggcagtatct aagcgatatt gaaacaggaa aagtcaagcc
     2641 atcagaggat ttacagcagt ccctttggga agctctggaa cgcttcaatc ccgacgctcc
     2701 ccttgaaatg ctgtttgatt atgtaagaat tcgctttccg acaacagacg tacagcaggt
     2761 ggtcgaaaac atcttacaac tgaaactgtc ctattttctt catgaggact atggtttcta
     2821 ttcttattca gagcattatg ctttaggcga catattcgtc ctttgctccc atgaactgga
     2881 caaaggagtt ctggtggaat tgaaaggtcg tgggtgcaga caatttgaaa gctatcttct
     2941 ggcacaacaa agaagctggt atgagttctt tatggacgtt ttggtggctg gcggtgtgat
     3001 gaaacgcctt gaccttgcca ttaacgataa gacagggatt ttaaatatcc ctgtactcac
     3061 tgaaaagtgc caacaggaag aatgtatctc cgtcttccgc agttttaaaa gctatcgcag
     3121 tggcgaactg gtacgcaaag aggaaaagga atgtatggga aacaccctct atatcggttc
     3181 attacaaagt gaagtttatt tctgtatcta tgaaaaggac tacgagcagt acaagaaaaa
     3241 tgatattccc attgaagacg cagaagtaaa aaaccgtttt gagattcgat tgaaaaatga
     3301 gcgtgcctat tatgcagtcc gtgatttact cgtctatgac aatccagagc ataccgcctt
     3361 taaaattatc aatcggtata tccgttttgt agataaagac gattccaaac ctcgttctga
     3421 ttggaaactg aatgaagaat gggcttggtt tattgggaac aatcgtgaac gattaaaact
     3481 aaccacaaaa ccagagcctt actccttcca aaggacgctg aactggctat ctcatcaagt
     3541 tgccccgacc ttaaaggttg cgattaaact tgatgaaatc aaccagacgc aggttgtaaa
     3601 agacattctc gaccatgcga aactgacaga ccgacacaag cagattttga agcaacagtc
     3661 agtaaaagaa caggacgtga taacaacaaa aaaataactc aaatacaaat tcattgaata
     3721 tagagaggag aacattttta tgaattttgg acaaaacct
//
LOCUS       DAHEYX010000204         3746 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHEYX010000204 DAHEYX010000000
VERSION     DAHEYX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.206"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..408
                     /locus_tag="MB219_002380"
     CDS             <1..408
                     /locus_tag="MB219_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428076.1"
                     /translation="IGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEE
                     LYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNT
                     DSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            422..1111
                     /locus_tag="MB219_002381"
     CDS             422..1111
                     /locus_tag="MB219_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428077.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1236..3587
                     /locus_tag="MB219_002382"
     CDS             1236..3587
                     /locus_tag="MB219_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCC8428078.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1279 a          484 c          779 g         1204 t
ORIGIN      
        1 attgggggct attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat
       61 caaagagtag ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg
      121 tcagatgaag aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt
      181 attccaggaa ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg
      241 ggagatggca aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt
      301 aatactgatt ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga
      361 aatgaattaa agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat
      421 tatgaaaaag atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc
      481 agtatatgct gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt
      541 tgctgatttt gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg
      601 ggaagctaaa attgtatcta tggataatcc agatataatt caaggttcat ataattcaga
      661 aactgatcct gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa
      721 aaatgatatt gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac
      781 tagcggacac cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg
      841 tgcgggatgg cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt
      901 acgttggaaa tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac
      961 taaatctggt tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc
     1021 accaaacggg cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc
     1081 aggtacgtat tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc
     1141 tgttttatta tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa
     1201 cgaacgtatg ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc
     1261 agaaatggct aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg
     1321 ggcgaacagg gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta
     1381 ttactaatct agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta
     1441 ctcctaaatg gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt
     1501 ggtgtaaggg gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg
     1561 ctgtaaaaga gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg
     1621 atgcgaaatg ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag
     1681 atagtaaaat tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa
     1741 taatgccttg tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac
     1801 aagctgaact aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta
     1861 gtaaaactta tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg
     1921 gattatatgt taatggttat tttgaagacg gtacattcga tggtattttc actaaatata
     1981 tgggtttaga agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag
     2041 cagatgttgt tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag
     2101 atggcgcaga tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg
     2161 atataaaaat agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa
     2221 ataagtatct aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc
     2281 ctatctatca agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag
     2341 atggtaagaa ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt
     2401 attttgctgt tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt
     2461 ttaagggggt atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa
     2521 gaaatgtttg ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata
     2581 tgtcgactgg ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc
     2641 aatttgttga atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta
     2701 catcgggtag agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca
     2761 ttgccaaata caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac
     2821 aagctggtct tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt
     2881 ctggtttgtc atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg
     2941 atgaatatag gaataaatta actaatagcg agggcctgta taattatttt atagatccta
     3001 atactggttc aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc
     3061 tagccgacac aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga
     3121 cttgctgggg tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag
     3181 taggtacggc tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg
     3241 taaatgttta taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa
     3301 cgtactattg tgataagggt gctattgata gatttaatca atttgttaag aacagatttg
     3361 gttcttcaga taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg
     3421 atatcttagg tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg
     3481 ggacagattt tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt
     3541 ctgaaaaaat tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta
     3601 tttaaacagt gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa
     3661 aaggacatga tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat
     3721 aattctggat gtggaattat ctttat
//
LOCUS       DAHEYX010000205         3731 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHEYX010000205 DAHEYX010000000
VERSION     DAHEYX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.207"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..312)
                     /locus_tag="MB219_002383"
     CDS             complement(<1..312)
                     /locus_tag="MB219_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428079.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..1018
                     /locus_tag="MB219_002384"
     CDS             434..1018
                     /locus_tag="MB219_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428080.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1029..1274
                     /locus_tag="MB219_002385"
     CDS             1029..1274
                     /locus_tag="MB219_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428081.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1280..2002
                     /locus_tag="MB219_002386"
     CDS             1280..2002
                     /locus_tag="MB219_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428082.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2133..2876
                     /locus_tag="MB219_002387"
     CDS             2133..2876
                     /locus_tag="MB219_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428083.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2882..3247
                     /locus_tag="MB219_002388"
     CDS             2882..3247
                     /locus_tag="MB219_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428084.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3263..>3731
                     /locus_tag="MB219_002389"
     CDS             3263..>3731
                     /locus_tag="MB219_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428085.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFM"
BASE COUNT         1454 a          498 c          656 g         1123 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg aacttaggga
      961 atatgtgcag aacccatata attttcaata tttagcagga agaataaatt tagtttagga
     1021 gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt gaaggtgtta
     1081 ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc attcaaaaaa
     1141 atccacaata cctattattt agtctttatg cttggattgt acaaatggtt tgggtttcca
     1201 tattgatgct gttgttttat tggatagtac gggttattct tttattgtta aacaaaagaa
     1261 aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt tatcgacagc
     1321 agcattaata acccttacag catgtggtac aactaatagt gctaataata acaaagacac
     1381 aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa caacaatgtc
     1441 ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta ataaaggtca
     1501 aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg aaaattatgg
     1561 gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg attgtccaga
     1621 ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta gttatgctcc
     1681 aacattaatg catgatatta ccataaataa accagcttac gagtattacg cagttgctgt
     1741 agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg ctgatgagaa
     1801 caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta acggcacact
     1861 atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg aaagtacaac
     1921 taaatattat atggatttgg gagcatataa attatatcaa caatcagagc ctgttatgga
     1981 cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt aaaattaagt
     2041 gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga aacacacaag
     2101 ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca aatgaagctc
     2161 atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa ggaatttata
     2221 aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta aaggcaacaa
     2281 gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca agtgaagaaa
     2341 tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa aaacatctac
     2401 tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa aaattaagaa
     2461 ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc ttaaatgact
     2521 acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac acacaataca
     2581 aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg tatgagatga
     2641 aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg aatcaagcga
     2701 ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg atcgtaaatg
     2761 aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat
     2821 caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg agatgagtta
     2881 gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa aaatacagat
     2941 gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg atcatattgc
     3001 taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga tacatcacaa
     3061 tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat atgatctatt
     3121 agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg cagagttaaa
     3181 aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga taatgcaacg
     3241 aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac aattaataga
     3301 agaagattta gagaccacag ttcagagatt agcggaacga atccattttg caaattatca
     3361 aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa tgaaattttt
     3421 ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga agaccatgat
     3481 ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag aggcaaaaga
     3541 aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga
     3601 tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa tagattttat
     3661 tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag atcatgaaca
     3721 gttgtttatg a
//
LOCUS       DAHEYX010000206         3699 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHEYX010000206 DAHEYX010000000
VERSION     DAHEYX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.208"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(5..916)
                     /locus_tag="MB219_002390"
     CDS             complement(5..916)
                     /locus_tag="MB219_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCC8428086.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1373..1801)
                     /locus_tag="MB219_002391"
     CDS             complement(1373..1801)
                     /locus_tag="MB219_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428087.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1829..2467)
                     /locus_tag="MB219_002392"
     CDS             complement(1829..2467)
                     /locus_tag="MB219_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8428088.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2471..3241)
                     /locus_tag="MB219_002393"
     CDS             complement(2471..3241)
                     /locus_tag="MB219_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCC8428089.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1104 a          708 c          555 g         1332 t
ORIGIN      
        1 ccgcttattt actacttgct gtactagaat ctgaagtgaa tgtactcaaa acattttcaa
       61 agtctttgtc tttgattttt acattagcgt ctttcaatac ttcgccaatt actttattct
      121 ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc tttcaattct tttttgtatt
      181 tatccatgtc gttgcctttg ttttggtttt tcaccatttt aaccacataa tattccgtag
      241 tataagttga agcattggta gaagtgatca catcagaaac ttgaccatct ttcaacttaa
      301 aggctgcttc tttgacttct gccggaaccg ttgtagtagt ggaatcaaat ttcacttttc
      361 cgccatcttc ttttgtcgta gtatcagttg acttgtcttt ggctaattta gagaaatcgt
      421 ctcccttatc agcagctttc ttcacatctt ttgcatcatc ttcacttgct acttggataa
      481 tttgagcttc cacttctgga tggaaagatt cccaagccgt ttttaaatct tcatcggtca
      541 atttgatatg tttctttaga ccttcttgaa aggctagact ttgtttgata ctgtctttga
      601 atgtttgttc tgtgtaacct gcagatttca attgtgaatc aaatgaatca cccaattgtt
      661 tctttgtttg atcatattga gaatcgattt ctttggttga tacgtcatct ccatatttat
      721 cttcaaatac ttgataaacg atcaactgtt gcacagcgga atctgctcct tgcatttttt
      781 tcaagctatc cattacttct tgttcgctga ttgtaccgcc tttataacta acggctgttt
      841 tttcaccggc attcgtacag cctgacaata agccagtgcc tagtacgatg gcaagtgcta
      901 aaaatctttt tttcatttat caattctcct acttttcgtt ttttgcgaat tgctaaaaac
      961 aaaggtgttc aagatgaaaa tcatcaagaa cacctttact aatctgctcg atacgtcctg
     1021 aatagatata aggctacaca atccagaaag ttcgctcagc agaataaatg tcaagtagca
     1081 catcacacct taatatacta cttcttttgt taccgttttt caagtctatc ttgcgttccg
     1141 tatttaacgt aactaattct tagaacaaat gttatcgatt ttgtttttct tcttccgctg
     1201 ctgggatatt caaatcagac acttttatgt tcgattttcc agacattttt ttgttgtttt
     1261 tattatgata tatttttata caactaaaaa tgaatatatt aaaactacca aaaataactt
     1321 ttaacgctgt ttcattatat gttttttcta ttttatataa acttttggat acttaatacc
     1381 aaccggttgg attgtaccaa catgagaatg tgtcagttct atttcctgtc gcacttgcat
     1441 aggcccatac tccttttgtt gcatttgctg atcgaataga gtttccaact ttcgcttttg
     1501 caccgtgacg attatagaca cgactatagt ggttagaatg acctgttttt ctcgtacaag
     1561 ttgtatcact ataataaacc catcttccag agctatgatt aattgctgtt ctagaaattt
     1621 cccggtctac tggtaaattt gacttaataa tattaccttt agcatcaaaa tgaatctctc
     1681 ctggctttaa agatttgtaa tagatttcac cgtgaactga atttgcttcc acttcagatg
     1741 acatagcgtt tcctccaaca aaacctatcc ctagtgcaca aataataact gcttttttca
     1801 taaaacgaca tcctttcctt tttgattttt aaagcatgat cttcttgttt cctattttgg
     1861 tcagatatgg atcatgcgtg actacgataa ctaacgtacc ttgggaagct tgttgcgata
     1921 agatttcaac tactttatcc ttatttcttt tatccaatga cccagtcggt tcatcggcta
     1981 aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac
     2041 cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct
     2101 catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta
     2161 aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagatatgg
     2221 tatttctttt tatcaagcgc ttctctctcc cagttaattg tttcgtctct tgatcgaaca
     2281 aatagaattg tcctgaactg aactgatcat tcaatcctaa tatattcaat aacgtacttt
     2341 tacctgctcc actttctcca acaacagtca caagatcccc agcatccgca gtgaaacgat
     2401 ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg
     2461 gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa
     2521 taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt
     2581 taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt
     2641 tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag
     2701 cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga
     2761 aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt
     2821 tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact
     2881 gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta
     2941 acggtggata gatttctccg ctgagaatat ttctttccaa atacatactg tttttcaatg
     3001 tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa
     3061 tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat
     3121 attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca
     3181 atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca
     3241 aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt
     3301 cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat
     3361 ataattcctg atctgctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt
     3421 tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct tttggtggtt
     3481 catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa
     3541 cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga
     3601 acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccat
     3661 atattccgct gaacaagaaa acgtacatct ggaacaaat
//
LOCUS       DAHEYX010000207         3589 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHEYX010000207 DAHEYX010000000
VERSION     DAHEYX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.209"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            507..713
                     /locus_tag="MB219_002394"
     CDS             507..713
                     /locus_tag="MB219_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCC8428090.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            713..937
                     /locus_tag="MB219_002395"
     CDS             713..937
                     /locus_tag="MB219_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428091.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            952..1389
                     /locus_tag="MB219_002396"
     CDS             952..1389
                     /locus_tag="MB219_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428092.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            1382..2089
                     /locus_tag="MB219_002397"
     CDS             1382..2089
                     /locus_tag="MB219_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428093.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            2470..2649
                     /locus_tag="MB219_002398"
     CDS             2470..2649
                     /locus_tag="MB219_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428094.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2742..2927
                     /locus_tag="MB219_002399"
     CDS             2742..2927
                     /locus_tag="MB219_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCC8428095.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2961..3392
                     /locus_tag="MB219_002400"
     CDS             2961..3392
                     /locus_tag="MB219_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCC8428096.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         1273 a          595 c          746 g          975 t
ORIGIN      
        1 gttattgcta gcatgtatgt tagaaatccc gatcatttta cacgatcgat gggcatataa
       61 ataaaagaag agttgtagtc ctaaatgagg attgcagctc tttttgtttt acatagaaac
      121 gggaacagga aaaggcctgt ttaggaaggc acaagcgcct tctcaattaa aaaacctttc
      181 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta
      241 agatatggtg tcttgcaaag caagacaaaa taggaaaagc ttcctctcac caaaaggttc
      301 gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac
      361 caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt
      421 cgttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc
      481 tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattattgg ggtgttccct
      541 tcagcgaatt attcccaaac tatcatgcac cacatactgt cgaatgcgat tgtggagaaa
      601 gagcgaaatg tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata
      661 ccttgtctta tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac
      721 acaacacaaa ttattgaatg taaaagaagt catttctgaa aaagtagcag aagctttact
      781 tattccgcgg tactggacga tagaaacccc tttagtagag gacgcaccga ctatgctatt
      841 tgaagaccta gtgaagattg caggatatga actgcattat caagaaaact tggaaactta
      901 tgaaaacgaa tattggttaa ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag
      961 aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat
     1021 atgacgttcc tataccatga aacacgtgta cccaaaaaac attacaagga aaaattgtca
     1081 gtcgcagtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat
     1141 cgaacattgg aagaactgaa aaaacaaaat ggaaaatggt ttttccaagc cttactatgc
     1201 ttggaagcag gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta
     1261 acgtatggaa aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttggac
     1321 tactttgaaa atgtcaacaa gtatggcgca tgttacatca tgaaaaagga ggacaacgaa
     1381 aatgagtaat gtggtttatc aactaacagt ggaagatgtt tctcactttt taacaaacaa
     1441 agaacatttg aatgaagtag tcagacggat tcaaaaagac tttagttgta tggaagaaat
     1501 gaagtacttt attcaaagag tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac
     1561 gaaggtcaca gtcagccata atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa
     1621 aaaaggaaca gagcttctac gactagaaac agaaaaatac atcatgaatg tgtacctaga
     1681 aaggagtgag ctggtcgaaa actttgtcag ctacacgatt tgtaggaaac actattactc
     1741 agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag aaatggaaga
     1801 gctaaacagc aaaaatgcga cggtagacga ttattacctt gtatttacta gatttctgac
     1861 gtgtgggacg gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagc
     1921 atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt
     1981 aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa atgagcttga
     2041 ctgtaaacga atgccatatc aacgatttga aaggaaaaaa ggtcaataac catttatacc
     2101 gaaaagtgag aatgtgtgaa ataagaacaa aaaaccttga aattaaggga atggttctta
     2161 tttcgcacca atcgctccaa ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac
     2221 gaccgataac ctaagagaaa gaaggaaatc catatgaata aaatcattcc tctgttgttc
     2281 gtactgtttg ttggcgagat acttatgcgt catgctgcag ctgtacattt aaacttcgta
     2341 gtattcttag gaatactggt attttgtttg atcttttggc gatttgtatt agcccttgtg
     2401 agtctactat tcaagatgat tgcagccata tttttcatag ggttatttcg atgaaaggag
     2461 agagtactaa tgccaattgc aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag
     2521 agacaaaaag aattgcagga actcaaacag aagtacggca aaagtgaatc caggattatg
     2581 tgtattgcgt tagatttatt gattgcacaa gaaaaagcag ggtttgatgt accagcactc
     2641 aaaaagtgac gtcacttttc atttgaagaa acaaaagtga tagcacttta cgcacgtgtc
     2701 gatacgtgtt atgattattt tgtaaggagg tatagtgcac aatgccactt acgggaaaag
     2761 aaatgcttaa gttattgaaa aaaaatggct gggttgagcg aaggcaagag ggatctcatc
     2821 atcatcttta taaggatggt gttagaatca ctgttcctgt tcatgccaat caagatcttg
     2881 gccgaggact tgagcaaaaa attttaaagg atgcgggatt gaaataatct cgcattcgat
     2941 taaagaaagg attgactgaa atgttaagtg agcctttagc aaaaacctat cctgctattt
     3001 ttagcccaga agaaggtggt gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca
     3061 caggaattaa cgaagacgat atatcttatg gaattgctat ggcacaagag gtgttaggta
     3121 tggttttagc agattatatt gagcatgaag atctacttcc agaaccaaca ccaatcaata
     3181 agatttctgt tgaagatgat tcttttacta ctttaattcg tgtagatgtt gccaaatatt
     3241 tgaaagacac agaacttgtc aaaaaaacct taacaattcc tcaatgggcg gataagctag
     3301 gcaaaagggc tggcataaat ttttcagtcc ttctaacaga atcaattgca gataaagcag
     3361 ataacattct tcgttctgga agaaataatt agaactcaaa agagtcagaa acttaaaaat
     3421 ctttggcttc tttttttact acaaagatgg cttctttaaa ttatttgcct gattgattta
     3481 aaaaaattgc gcaaagacta cgagctaatt aagttgctcg tggtctttct ttatgaaaac
     3541 tttttagatc tagataaaaa cattgagaca ctttgaaaaa tttttttaa
//
LOCUS       DAHEYX010000208         3563 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHEYX010000208 DAHEYX010000000
VERSION     DAHEYX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.210"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            70..2132
                     /locus_tag="MB219_002401"
                     /pseudo
     CDS             70..2132
                     /locus_tag="MB219_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
     gene            2215..2784
                     /locus_tag="MB219_002402"
     CDS             2215..2784
                     /locus_tag="MB219_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8428097.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2959..>3563)
                     /locus_tag="MB219_002403"
     CDS             complement(2959..>3563)
                     /locus_tag="MB219_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428098.1"
                     /translation="QIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWDTFLHAFSGFL
                     AGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSADKLLQFDMQK
                     DTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGYLDVGIMDTMK
                     DLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         1274 a          644 c          662 g          983 t
ORIGIN      
        1 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
       61 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      121 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      181 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      241 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      301 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      361 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      421 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      481 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      541 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      601 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      661 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
      721 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
      781 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
      841 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
      901 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
      961 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1021 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1081 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1141 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatatagatt
     1201 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1261 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1321 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1381 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1441 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1501 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1561 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1621 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa gaagaaagct
     1681 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1741 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1801 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1861 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1921 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     1981 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2041 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2101 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2161 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2221 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2281 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2341 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2401 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2461 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2521 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2581 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2641 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2701 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2761 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2821 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2881 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     2941 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3001 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3061 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3121 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3181 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3241 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3301 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3361 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3421 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3481 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3541 ttctagcata tttggaatct gta
//
LOCUS       DAHEYX010000209         3483 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHEYX010000209 DAHEYX010000000
VERSION     DAHEYX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.211"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(128..688)
                     /locus_tag="MB219_002404"
                     /pseudo
     CDS             complement(128..688)
                     /locus_tag="MB219_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369693.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            922..2214
                     /locus_tag="MB219_002405"
     CDS             922..2214
                     /locus_tag="MB219_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCC8428099.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            2472..>3483
                     /locus_tag="MB219_002406"
     CDS             2472..>3483
                     /locus_tag="MB219_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HCC8428100.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSTIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KST"
BASE COUNT         1165 a          649 c          627 g         1042 t
ORIGIN      
        1 attttagata atatttctat taattatgtt taattgattg cattttgtag agagtgggac
       61 aatagtcctt tctccaaaag aaaacaagtc cgattttgat ggttaatttc aaaatcggac
      121 ttgttttttc ttcttttaaa taaggcaaga tttcttcttt ataaagggca taatagaatt
      181 cgtttgattg cttatccaaa gcagtttcat aacgattcgt ttctttacgc cagaagaaac
      241 tgaacttgtt ggcatctgct tcaatttttg taccatcaat aaatattgct tcattatcaa
      301 ttacttaatt ggtgatcgat tggcggctaa ataagacaaa cgcttcagct aaaaggtcag
      361 cgggtgtttc ctgacttcgg aatcgattga tggtccgata gctgacttgt tcgtggttag
      421 ccaaccaaca catacgatag ctgtcatcta atagaaattc aattttccga ccagaaaaga
      481 cacgctgcat ataagcatat agaataattt ttaacatcat tttggggtgg taggccggtc
      541 gtcctagtta ttcttataat tcaagaaaga gtgtttgtgg cattgctacg actaaagcgt
      601 tgactgcaaa agcgacatct gtttcaggaa tatattgttc catatctaat ggtaaagttg
      661 tttgattgat gggataattt ttaaacataa agtactcgct tcctttctga ttgggcttgg
      721 tcactttaat cttacaggga tgagtgcttt ttgtgtacct taaaatcaaa aaaagtgggg
      781 caataatcag attttggctc tatacttttt gatgttggtg gatgacacca gcatcttttt
      841 tattttccaa aacatataga aaagacacct tgaaatcctt tagaattaat ttggcgaacc
      901 aacccaaaag gaggaatcaa gatgtcttac actcacctta tcaaagaaac attagatatt
      961 ctagacttat cagttacttt taacgaaaat tgtctcacaa aagaaaaata caaagggcaa
     1021 atctgtcata tttatcgtgg aaacttgatt tatacagccc aagaatgcat ccactgtaaa
     1081 caccagattg cttcagatat tgtgcgttgg ggtacaacaa ccgtccggtt gttaatgaac
     1141 gatgtttctg aataccgtac ctacctcgaa ttgaaaaagc aacgcttcaa atgcaaagct
     1201 tgtcagcgga cctttgtcgc agatacttct gttgcgaaaa aacattgtct catcagtgag
     1261 aaagtccgct ggtctgtcgt gactcgtctg aaaaaaaata cctcaatgac agagatcgcc
     1321 gctcagaaaa atctatcggt ctcttctgtt taccgtatta tgaagcgatt ttatcgtccg
     1381 ttgaatcctt ttcggacacc gttacctaag gtactttgtt ttgatgaatt caaatcagtt
     1441 cggggtgttt caggtgctat gagtttcatc atgatggatg gacaaacaca gcgcttattg
     1501 gacattgtcg agaaccgaca actgcctttt ttaaaacgtt attttagtca tttttcacga
     1561 gaaattcgtg aagcggtcga atggatcgtg attgatatgt acgctcctta tgtatcgctc
     1621 gttaaaaagc tgtttccaaa agcacagctg attattgacc ggtttcacat cgtccaacat
     1681 attggtcgta cctttcgcaa tcatcgaatc aaagaaacga accaactatt aaaaagcaaa
     1741 gaacaaaaac actatcaatt aggtaagcaa ttaaaaagat actggaaact tcttcaaaaa
     1801 gatgaacgca agcttgatta tattcgacgc ctttggcgac ctggcttcaa agctcattta
     1861 acagagaccg atattgtgga tcgcttactg aaaaggagcc ctgctttgcg tgtaggctat
     1921 cagttatatc aagactttct ttacgctgta aaggaacgag actacgtatc gtttgaagaa
     1981 ctacttacca ataatatcat gcttcccgaa ggctatcaga cgaccttgag aacgttccag
     2041 aaatttctgc cacaaatcaa aaatgcttta caacaaagtt attcaaatgg cccacttgaa
     2101 tgtctgaaca accatatcaa agtgttaaaa cgaaatgcat atggctttcg tagcttttat
     2161 aatttcaaat tgcgcatcat gattcgtcat ggcaacgcat tgattttcaa ctaaaaaaga
     2221 tcaagaggaa ctgatccact tgatctccaa ttattctaag atttctcttt accaacatca
     2281 tttgacaaag agccacataa aatagcaaat aatatcctaa aacagctatg attttttcct
     2341 tacaaagtat acatttctga ttgaatttat gataaagtcc gtataattgt gtaaaagtaa
     2401 aaaggccata taacagtcct tttacggtac aatgttttta acgacaaaaa catacccagg
     2461 aggactttta catgacccaa gtacatttta cactgaaaag cgaagagatt caaagcatta
     2521 ttgaatattc tgtaaaggat gacgtttcta aaaatatttt aacaacggta tttaatcaac
     2581 taatggaaaa tcaacgaaca gaatatattc aagcaaaaga atatgaacga acagaaaacc
     2641 gacaaagtca acgaaatggc tattatgagc gcagctttac gacacgtgta ggcacgctag
     2701 aattaaaagt acccagaaca cgtgatggcc atttttcacc cacagtgttt gaacgttatc
     2761 aacgaaacga aaaagccctc atggcttcaa tgttggaaat gtatgtatca ggcgtttcaa
     2821 ctcgtaaagt atcaacaatt gtggaagaac tttgtggtaa atccgtctct aagtccttcg
     2881 tttctagctt aacagaacag ctagaaccta tggttaacga gtggcagaat cgtttattat
     2941 cagaaaaaaa ttatccttac ttaatgaccg atgtactcta tataaaagta cgagaagaaa
     3001 atcgagtact ctcaaaaagc tgtcatatag cgattggaat aaccaaagat ggcgaccgtg
     3061 aaattatcgg cttcatgatt caaagtggcg aaagcgaaga gacctggaca acattttttg
     3121 aatacctaaa agaacgcggt ttacaaggta cggaactcgt tatttctgat gcgcacaaag
     3181 gattagtctc tgccattaga aaatccttca ccaacgtaag ttggcaaaga tgccaagttc
     3241 acttcctaag aaatatcttt accaccattc ctaaaaaaaa ttcaaaatct ttcagagaag
     3301 ctgttaaagg aatttttaag ttcacagata ttaacttagc gcgtgaggct aaaaatcgat
     3361 tgattcatga ttatatcgat caaccaaaat attcaaaagc ttgcgcatca ttggatgatg
     3421 gattcgaaga cgcctttcaa tataccgtac aaggaaattc ccacaatcga ctaaagagta
     3481 cca
//
LOCUS       DAHEYX010000210         3469 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHEYX010000210 DAHEYX010000000
VERSION     DAHEYX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.212"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(92..769)
                     /gene="nth"
                     /locus_tag="MB219_002407"
     CDS             complement(92..769)
                     /gene="nth"
                     /locus_tag="MB219_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCC8428101.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(776..1474)
                     /locus_tag="MB219_002408"
     CDS             complement(776..1474)
                     /locus_tag="MB219_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCC8428102.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1742..2926
                     /locus_tag="MB219_002409"
     CDS             1742..2926
                     /locus_tag="MB219_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCC8428103.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2932..3375)
                     /locus_tag="MB219_002410"
     CDS             complement(2932..3375)
                     /locus_tag="MB219_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCC8428104.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          884 a          702 c          587 g         1296 t
ORIGIN      
        1 tactttttca tgcaaaaaag ataaacgaaa cagcttttct ccataataac aagataattc
       61 atacatttat ttgcaattaa agaaaccacg tctacaggcg tggtttcttt tttagcgcct
      121 tttcttttag acgcatccga tttttcccat cttgacatat tgatagcaaa ggacatacct
      181 cgcacttggg atttcttgcc gtgcaatgat atctaccaaa aaaaatcagt gtatgatgtg
      241 tctttaccca taattcttgt ggtactttcc gcatcagtgt ttcttctact tccatgaccg
      301 tagcatctag tttgcagatc ctcaggcgtt ttgagacacg ttccacgtgg gtatctactg
      361 cgattgctgg tatcccgaat gcatccccca atacgacatt tgctgtcttt ctgcctacac
      421 caggaagaga catcaattct tctcttgacg ttggcacttg cccatcgaaa cgttcaatca
      481 gctgctgtgc acacgctttg atgttttttg ctttatttcg atacaaacca atcgttttta
      541 ttttaagaat gatttcatcg attgaagctt ctgctaacgc atcgggtgtc ggaaaagagg
      601 caaacaaatc tggcgttgct ttattcactg atacatctgt cgcctgggca cttaagatca
      661 ctgcaatcaa taactcaaat ggattattgt gcttgagttc tccatgtgct tcgggaaaca
      721 ttccatacat cgtttctaga gcttccattg ttttttgttt gcttaacata ttccgctact
      781 cctccaacca atttttcagt gagactttcg gcaattcttc ttgttgagct gactgttcta
      841 cttctctttg aagaagctgc tgttttcttc gtttttggtc ttcttccact tgttgttttg
      901 tacgtagatt ttttctttcc cacgatagca aaatgcggtc aacgtaatta aaactataag
      961 cctgatttaa tacggcttcc cgcagtgcta gcttcaaaat ttccggctga taatggtctt
     1021 cttctagcca ttgtccgatc cgctggaact cgatcgaaga aagcgggcgt ccaaattctt
     1081 gttcaaataa ttgatacact gactggattt ctttttcttg tgttttttct tcgtattttt
     1141 tttcttgtgt ttttaaatac tcacctaata gatcatagat ggggtaaaga ttatatcgat
     1201 ctgctttttt accgttgaca atcgtcgttt ctatcttgat aaatccattt gttacgagac
     1261 gatccagtat ttgaaaaatc tgatcttgtt tgattcccat atccatagca atcatctgca
     1321 aatcagggaa agtatctcct tcaagctgtg ccatatgcaa ctgtagaaca aataaaaatt
     1381 cttcagattg tagaccgatc ctatgataat ttttaagtaa aagattagat atgactgttt
     1441 gtccagcgct taagtaattt tctaaattca acatgatttc tcctccgctc aatcacagta
     1501 tacgtaaaaa aaacatggga agcaagaaca ggtttctttt ctatttactt cactgatcgg
     1561 tcagatttac aatgtcgtct acttgatttt tgtttgttca tgtttttgat gcgtatcttc
     1621 acttttcgga ataaataaat taagtagatt tcgctgttcc ttagaatgtt gacaaggaaa
     1681 caaaattact atacactatt tttgtttcca agttaacatt attgcataaa ggagttttta
     1741 catgtacagt ttcacaaata aacattctaa agtttttgga tacgtctctg tcttattcac
     1801 tggattagga tttactatcg tcagtcctgt acttccgttt ttaacgctgc cttattctca
     1861 ctccatacat cagcaagctt tctatatcac tttgctcatg tctgtttatg ctctagctgc
     1921 cttcttatct gcccccattc tcggtagctt aagtgatcat tttgggcgtc atccgatcct
     1981 tattatcagt ttattaggat caagtatcgg atatttgctt tttggtttag gaaattcgat
     2041 atggatgttg ttccttggtc gtatcattga aggattgacg gcaggagaaa tctccacctt
     2101 atatgcttac tttgccgaca tcacggaacc taatgaaaga accaaagttt ttggatggat
     2161 gggtgcttta gtcggtatag gaacaacgct gggtcctatt atagggggtc tattagctga
     2221 gttggggaat tctgttccta tatttattgg agcactcttt actttcttaa atgccgttta
     2281 tggctacaca tttatgctag aaagccttcc aataaaaaaa cgttcagtag atttaagttt
     2341 ctcccatgta agaccttttc accaattaca acaattattc aagatttcat ccgttattcc
     2401 tttattgaca gcaggttttg ctgtttggtt ggcagctggc tctcttcaat ctatcttttc
     2461 tcaattttcg attgacgcct ttcagtggaa ggctgggttg gttggtttaa gcttctcttt
     2521 gattggaatc ttggatactg tctcgcaatt gttcattatg cctcgattgc tcaaaaaatt
     2581 ttcagaacag cagatcacaa gaatagggat gttcagtgaa atcctcgcct atctattcat
     2641 cacattatct ggaattcttc tactgccgat tttgttttta tttgggatta tctgttatgg
     2701 tttcggtgat tctattttta ctcctgtatt caatggccaa ctatccaatt ctgtctcaga
     2761 taaccagcaa ggtcttgtaa tgggcggtac ccaaagtata caatctctat cgcgcgtcat
     2821 cggcccgcta atcgcaggac agctttatgc tgcagcacca tttcttccga cggtattcgg
     2881 atttttgctt atgatttttg cctattatca ctacaataaa aaatagtagc ttcatttgct
     2941 acttcgtata aattctctgt atcgaccgat aaaacgaaat actgtgtcaa attcttcttt
     3001 tgacatattt tcaaatacag tagcatctcg ctgtacccat ttttgatgga gttgctcgtg
     3061 tctattcctt attttttgtc cgattggcgt caattgaaaa tagatttctt ttttattctc
     3121 ttcgctttga taagtgttta tgatccctct agctatcagt ttttttgtta gcttactgat
     3181 ggctcctcta gtcatgtaac tagcattcgc taattttgtc acgttaggat cagggatttt
     3241 cccgatatgt tcaatcactt ctagttcatt tagagtaaat cctgcaaatt ctttttccat
     3301 ttctggttta ttacgatgaa taatctcatt agctaaatcc agatattctt ctattagcag
     3361 ttgtctttta tccatttcta caccttcttt ttctttcatt atattgtttt tttgtttcct
     3421 attcaacaaa gataaaaata ttttgtagga aaacccaatc tgaaaccaa
//
LOCUS       DAHEYX010000211         3396 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHEYX010000211 DAHEYX010000000
VERSION     DAHEYX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.213"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(291..1127)
                     /locus_tag="MB219_002411"
     CDS             complement(291..1127)
                     /locus_tag="MB219_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCC8428105.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1138..2067)
                     /locus_tag="MB219_002412"
     CDS             complement(1138..2067)
                     /locus_tag="MB219_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCC8428106.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2259..2612
                     /locus_tag="MB219_002413"
     CDS             2259..2612
                     /locus_tag="MB219_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCC8428107.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2686..3342)
                     /locus_tag="MB219_002414"
     CDS             complement(2686..3342)
                     /locus_tag="MB219_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCC8428108.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
BASE COUNT         1074 a          700 c          570 g         1052 t
ORIGIN      
        1 caaaaaccaa actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc
       61 gatattttac tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt
      121 gtaattctct tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat
      181 gctgcttcta aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc
      241 gacgtccttg cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa
      301 cgatacacat actgattttt cggagcagag actttctcag cgtttgtaac aactacagat
      361 gggatttcac gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac
      421 cattcatcat ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct
      481 gaatctgcca cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg
      541 ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg
      601 taattataaa ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc
      661 ttatccccat acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa
      721 taaatactgg tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct
      781 ttacttactg ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt
      841 ggaaacaacg tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga
      901 taaggttttg acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg
      961 tttttgaccc acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga
     1021 atagctaagt agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag
     1081 tacatcatca attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta
     1141 aataaataat gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa
     1201 gcgtcctgta aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa
     1261 gaccaaaaag gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa
     1321 cgcatctgct tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc
     1381 taaaatcttt gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc
     1441 agctgcgatt aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga
     1501 atgtgtcaac acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact
     1561 ttgttcttct tcatggacat gatgatggtg attttctgct actttagacg tgattttttc
     1621 tttcaataca gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa
     1681 aaagctgcct atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt
     1741 ggaaaaaatc acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg
     1801 aactggtacg ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga
     1861 aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag
     1921 ccatctcttc acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc
     1981 taataatacg aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa
     2041 aaccgaattt ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc
     2101 tttgcaaata tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact
     2161 cttatttttt accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc
     2221 tcatacatat cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga
     2281 ctgaagttca agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta
     2341 attgcagtgt ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata
     2401 ttccggcact tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc
     2461 tgactgcgcc agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt
     2521 tcatcgactt taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc
     2581 tttcttatga agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg
     2641 aacaagtgaa agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt
     2701 ttcggaatgc gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt
     2761 caaatcaaca ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt
     2821 caagattcct ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc
     2881 gggtatcgtt aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc
     2941 cattggataa acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa
     3001 agcacaactg atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc
     3061 caagttccct acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc
     3121 aaaaaagtgc ataagatctt ctacatcata accataccca cgttttccta attcgccaaa
     3181 ataagaaaaa tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt
     3241 tgaagacact tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg
     3301 cagtctttta gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat
     3361 tgtctaatta catctttgat ttctccatca tagcag
//
LOCUS       DAHEYX010000212         3328 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHEYX010000212 DAHEYX010000000
VERSION     DAHEYX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.214"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            255..1994
                     /gene="yfcC"
                     /locus_tag="MB219_002415"
     CDS             255..1994
                     /gene="yfcC"
                     /locus_tag="MB219_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCC8428109.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2151..>3328
                     /gene="iadA"
                     /locus_tag="MB219_002416"
     CDS             2151..>3328
                     /gene="iadA"
                     /locus_tag="MB219_002416"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCC8428110.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT         1060 a          547 c          768 g          953 t
ORIGIN      
        1 atgaaaaaaa caggacgaga ttttctctaa tctaaaaata aaaaaagaat tttaaggaaa
       61 aacagagaaa taaaatactt aatataaatg gaaagagaaa aaaataatca tacataggag
      121 aaaaaaaaca aaatagaaaa aagtgatttt ctaataaatt attcatgtgg aaatgtcatt
      181 tttactgtat aattcatttg attatccatt ttttattcat caaaattact gaaaaaagaa
      241 aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc gacttcacta
      301 aattatcgac accccatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac
      361 tgacttttgt cgttccagca ggaaagttca gcacgcaaga tattgaatac aaagatgcaa
      421 atggcgaaac aagtactcgt actgttttac gtcaagattc cttccgttat gcttacgagc
      481 tggataagtc gtatgtgttt gatcaattag aagaactgca agatcatcca gcagaacgtg
      541 aaaaactgga tgttccggaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc
      601 ttacgcagga aaaactagat gaaatctcct tgacagatga tgtgctttac gatcagtacg
      661 gcgaaaatat ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag
      721 atgattttgg cggctttggt ttcttaaatt ttgtttttga aggactagta tctggagata
      781 agtatggatc tgctgtaggg atcgctgcat tgatccttgt tgttggaggt gcttttggta
      841 ttattatgcg taccggagcg atcgacgcag gtatctatgc gtttatcagt aaaactaagg
      901 gactggagcg tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat
      961 tcgggatggc agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt
     1021 taggatatga ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg
     1081 cgacctcttg gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg
     1141 tgttgtctgg tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg
     1201 gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca
     1261 aatcagatga ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca
     1321 tgcttgggca taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg
     1381 gcgtgactca aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat
     1441 tagttgctgg aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg
     1501 cttcttcttt ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg
     1561 ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata
     1621 cgattttgca tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg
     1681 cgatgtacat cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag
     1741 ctttgactat gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag
     1801 cagttcttgc ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca
     1861 gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa
     1921 tcaagatgca aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa
     1981 taggtctgca gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg
     2041 actccaagaa ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt
     2101 attttttaag atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca
     2161 ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg
     2221 gtgggaagat tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag
     2281 aagaactttc aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata
     2341 ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc
     2401 aattgactac agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta
     2461 gagatatgac tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt
     2521 atgtatacga aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag
     2581 actttttgac aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt
     2641 cttctcagcc aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca
     2701 tgttatccgg aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag
     2761 agctattgac acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc
     2821 atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca
     2881 cgattgactt cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc
     2941 gcttcagcaa agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga
     3001 tgacctcaga tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt
     3061 taggagtagg aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa
     3121 gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat
     3181 tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag
     3241 aaacgcttga tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag
     3301 tcaaagtatg ggggactttc gaaaaaag
//
LOCUS       DAHEYX010000213         3316 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHEYX010000213 DAHEYX010000000
VERSION     DAHEYX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.215"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            217..429
                     /locus_tag="MB219_002417"
     CDS             217..429
                     /locus_tag="MB219_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428111.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(707..2506)
                     /locus_tag="MB219_002418"
     CDS             complement(707..2506)
                     /locus_tag="MB219_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCC8428112.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2630..3226)
                     /locus_tag="MB219_002419"
     CDS             complement(2630..3226)
                     /locus_tag="MB219_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCC8428113.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1213 a          600 c          587 g          916 t
ORIGIN      
        1 caatataaag aaaaaaacgc acgaacaaca taggaatata aacaaacgtt tttgtcacaa
       61 cgtctaagat cgtgttaaaa aatagttatc taaaaagcag aaagatactc aaaataaaaa
      121 ataaaaaaat ggataaaaac gttttcaaat acaaggtaat ttgttattat aaaataaaga
      181 aaaaacaaaa taaaaataaa gaaaagaggg aatgaaatgg taaaagcaga agtaggaaga
      241 tcttatatat gcaaaccgat cggagtatct catgaactaa tcggctgtat tgaacgtaca
      301 tatatcaaca cggctgtcgt gtatgttgaa agttatgacc aacgagacta tcaacttatc
      361 aatgaaagca agtatcgcat gctcgtaaaa ttctgtgata ttagcgcacc tgctgaattg
      421 gtttcttaaa cagtattttt gagaattcgc aagaactccc ttataatata aataaagaca
      481 gaagggagcg agaagatatg ccaaaaagaa aaagttcagg gaaaatggga attattcttg
      541 cagtgatctc tgcaattcct gctattttag ctattgttcg tgcgatgaag gaatcaagag
      601 aaagttaagc acacattatg agcgtcaaac aaaaaacgca tatacctgcg gtggcaggtt
      661 atgcgttttt tgttgtataa agactttttt cattaacgat ttttgattag ccgactccca
      721 aaacaaaatt tagcagttta tctgaataag tgatttcttc atctgtttgt ttcacacttt
      781 catttagtag tcccatctca tcggttatga taccatccac accataaaac atcatgcgac
      841 tcatagcatc tgaatcgttg attgtccata catatacttc ttttccgtct tggtgggcag
      901 aattaatgaa gttacggttg atcgtactat attccattgt cagaaaatca gcaggcgtca
      961 cagggggccc aacaatattg aaaggtaaga tgtagcccac ataaaagttt ggttctgctt
     1021 cttttaaatc tgaaacggtt tggtacgtca aactttgcaa aatatgatgg ttctctaaga
     1081 taactttgcg gtatttatgg acaaatcgtt cgacaaggtc atccgaatct tttttagagg
     1141 tcttgatctc aataagtaat ttttggttta gcttatgtgc tttttccaaa taagaatcaa
     1201 aagaacaaac aggggcttgc actccgtttt cagtaactgt aagatttgtc aattctttca
     1261 atgtaagatc ctgtggtgtt ccatttaccc ctgttaagtg acgaagattt gcatcatgca
     1321 tcactacgaa atgaccatct tttgtttcct gaacatccat ctctacataa tcaggatgat
     1381 agtctctgct tgttttttca agtgcttcta aagtattttg cgccccattt tgggtactca
     1441 cacctctgtg agagacagtc actggttttg tctgaacagc tgaagtcaga taatccatat
     1501 tgtaaagaca gacgccaata ccgaaaaaaa ctgcaaaaag agtcaaacca gtgtttttca
     1561 gcgcagagaa acgcaagttg ggggcttgtg ggataaacca cttagggatc tcaggcaaga
     1621 agccttcgtc atccatgaaa tcaacaatga tataaaaaat ccctacggta gacatcacga
     1681 tattgaataa taaaatccct tgtaaaagcg tcatagcaaa cacagcgctg atcaatgcat
     1741 agtctgggaa aaattgttca accatacttt gtgccaaaat aacaacgata tatcctaaag
     1801 aagaaaggag taaaatcgta ccgccaatca caataaattg gcccgtgatg gccaaaagac
     1861 gcgatttggt caatgaccag ctttcccgaa tggctgctcg aaaaggacga tcccgtagaa
     1921 tcatttccgg taaagcaaaa atcagtcgaa tcccaatata gccaaggaaa atataaacga
     1981 gtaaaaagga actgacgatg atccaacgat tagcaaaaat aaaatccata atgaaagcgg
     2041 ggatttttat ttttgacagt agatcagaat taaaacttaa tccgctgatc ggtagaatca
     2101 ataaaaaata agcaaggaaa aacaaaaaag taatgggtct cactttcttc agctgaagta
     2161 tcgtcaagtg taagagttgc ttcaaagaga ttggttcttt ctttttaata aaaaaaacac
     2221 tcatcaaaag aaaagtaaat tcgaaataaa ctaacagtaa aatcgtcatc aaaactacga
     2281 ttaacgagag tagaacccca ggatgttgag aaaaaattga aggaatattg tcataagaca
     2341 aatagtcaat tgcccctcgg tgtaaaataa aacgggtcgt actgctcaat aagggtaaac
     2401 aaataaataa aataaagcca tgcatcagca agacatcacg gaaataagct tgtgttcctt
     2461 ttaaaaagtc ccaagcatta cctaagctac gtttcaaata attcatccta tcacctgctc
     2521 ttcacattat agcgcaaatc taatgaaaaa ggaaagggtg cagtgtgttt tgcagaccct
     2581 ttcccagatt taacattgtc aatcaatgac taattttaca aaataaatat tatccaagaa
     2641 gaccacttag atattgacct ccataaatca aggccatact atataagaaa atcgacagtg
     2701 gtttagcaat caaaatgatt agtgtatatc gtttcactga catgtttgtc aatccagcca
     2761 tcaaacataa cgcatcgtca ggagcaactg gaagaaaaat agctagagca aacagcttct
     2821 caaaacggtt ttgattatcc agccagccaa tatatttgtt atatgtctta tcgctaatca
     2881 tggaaagtat gaatggcttt ccataacgac gccctagcag aaaaatgata atcgaaccaa
     2941 tgcagattcc aatatagtta tagataaatc cagcataagg gccaaatatc aggacaccag
     3001 ctgcagtact gatccctcca ggaatgattg gaataactac ttgcaggatt tggatgaaga
     3061 caaaaataat cggtcctaaa atgatggaat cacctactaa tcctcttaac gcattcaaat
     3121 ctttgaaaac acctaaatta ataaaataaa tcgtcagcgc gattgatagg cctaatccga
     3181 taatggatat gaaatttatc agttttcgag aggcagcaat attcattatt catcaactcc
     3241 ttagaggttc tttgttaact atactttact agaaaagcaa ctaactgcgc cattttttga
     3301 tatgacagaa aacaaa
//
LOCUS       DAHEYX010000214         3218 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHEYX010000214 DAHEYX010000000
VERSION     DAHEYX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.216"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            146..1105
                     /locus_tag="MB219_002420"
     CDS             146..1105
                     /locus_tag="MB219_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCC8428114.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            1144..1590
                     /gene="dhaM"
                     /locus_tag="MB219_002421"
     CDS             1144..1590
                     /gene="dhaM"
                     /locus_tag="MB219_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCC8428115.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            1565..2575
                     /gene="dhaK"
                     /locus_tag="MB219_002422"
     CDS             1565..2575
                     /gene="dhaK"
                     /locus_tag="MB219_002422"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCC8428116.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            2603..3208
                     /gene="dhaL"
                     /locus_tag="MB219_002423"
     CDS             2603..3208
                     /gene="dhaL"
                     /locus_tag="MB219_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCC8428117.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         1092 a          554 c          739 g          833 t
ORIGIN      
        1 tgggactgat gaatcagaat tgattcttca gccccatttt tattttaggt atcgccgatt
       61 gtaaaattaa gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgtcg
      121 aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaccaa ctgaactcgt
      181 aatgatagaa gcttatttca atcaatcaca ctctgtttca aaagtagccc atctggtaca
      241 acgttctcga caaaccattt ataatgtcta tcgttttctt aaatctggtg gaagcgcaat
      301 aagctactat gaacaatata agaaaaacaa acgtaactgc ggaagacgtc cgattgtgtt
      361 accagatggg caacaagaat acattcaaaa aaaggtggct caagggtgga ctccagatgt
      421 ccttattggt cgagcagagt ttcctattgc ttgttcggta cgtacactat atcggatgtt
      481 taaagataaa acctttgacc ctcacgattt accaatgaaa gggaaaagaa aaccaaacgg
      541 acataaagaa aaaagaggaa aacaagcttt tcgccgttcg attcaggata gaacaactga
      601 ttatatacag ttcaatcatg agtttggtca cctagaaggt gacaccattg tcggtggaaa
      661 acataaaagt gcggtgatca cgttagttga gcgattatct aaagtgatca tcaccttgaa
      721 acccgctggt aggcaagcta tagatattga gaatagactg aatcaatggt ttcaatctgt
      781 accgaaacac ttgtttaaat ccatcacatt tgattgtgga aaagagttct cgaattggaa
      841 aaagattagt aataccaatg atatttcgat ttatttcgct gatccaggaa caccttctca
      901 acgaggacta aatgaacatt cgaatggctt attacgtaaa gatggcttat ataaagctat
      961 ggattttaat aaggtatccg aaacctttat ccaatcggtg gcatcaaaac gaaatcatat
     1021 tcctagaaaa tcattacact atcgcacacc attggaagta tttttgagtt acgtaggtaa
     1081 agatgatttg tctagcttaa tttgacaaac gaaaaattta ttatatactt gaattgttca
     1141 ttaatgaaag cgaaaccaat cgttagcaac tatgtaaaag aaaggaagat tcttgtgaaa
     1201 aaaagtattt tgcttatttc tcatagtcaa aaagtaacag atggtattaa agaaatgatt
     1261 gagcagatgc aaaaatccga agaggtgcaa attttttctc tcggcggaat aaatggcgag
     1321 atcggatcag acccgatgaa aatcgtagaa gcagtcaatg aatcaagcga tgcagctagt
     1381 tattttgttt ttgcggatat cggtagcgcg gtattgaact cggaactggc aaaagacatg
     1441 ttggaagaag agcagcagca acgttatcat ctggtggatg cgcctttagt ggaaggagct
     1501 tttgcagcag ccatcactgc cggtataaca gatgatactc agcaaatctt aacagaagca
     1561 caaaatgccg gaaagaaagg atgggaataa attgaaaaaa atcatgaatg atccaagcaa
     1621 tattgtcgaa gaaatgctgg aaggattagt aaaaagctat ccagaacttg ttcatcgtgt
     1681 agaatcttcc cgtgttgtag ccaagaatca gaaagcggaa caagttggtt tggtttctgg
     1741 tggaggaagc ggacatgaac catcccatgc cgggtttgta ggggaaggga tgttaagtgc
     1801 agcggtgctt ggtgatgtgt ttacttctcc aactccagat cagattcaga cggctattaa
     1861 ggaagccgat agtggaaaag gagttttgct gatcgttaag aattatacag gggatgcatt
     1921 gaacttcgat atggcaaagg aattagctgc tatggacgat attgaagtag aaagtgtgat
     1981 cgtagatgat gatatcgccg ttgaaaatag tacgtatact gctggaaaac gcggagttgc
     2041 aggtactgtc cttgtacaca aaatcgtggg cgatgcagca agaaacggtg cttccctagc
     2101 tgagttgaaa gaactgggag aaaaggtcgt acaggcaact aagacgatcg gtctagcgct
     2161 tcgagcagca actgtaccag aagtagggaa accaggattc gaactaggag aagatgagat
     2221 cgagtatggt gttggtatcc acggtgagcc aggctatcgt cgagaaaaga tgcagccttc
     2281 taaagtactg gcaaaggaac ttgttactaa gattttagat gattactcga atttaccaaa
     2341 agaagcaggt gtactagtca acggaatggg cgggacgcct ttgatggaac agttcgtgtt
     2401 catgaatgat gttttagctc tgttggaaga aagaggcgtc aatgtagttt tccgaaaagt
     2461 cggtaatttt atgacctcac tagatatgca aggtctttct ctaacactga tcgacttgac
     2521 ggagacacaa tggaaagatt ctttagaaag caatgtacag acgatttcat ggtaaaaaat
     2581 ctcaagataa aagggtgata atatgaaact aaccgttcaa gaaatccaag aatggctcac
     2641 gcaatttgca gaggcaatca atcagaacaa acaatatctc agtgatctag ataccccgat
     2701 tggcgatgga gaccatggaa ataacatggg aagaggggtt tcagcttatg aagaagcttt
     2761 tcaagccgat catcctgaaa caatcagcga tacattcaaa gtattctcta tggcgatgat
     2821 atcgaaagtc ggtggggctt ctggtccgtt atacggatct gcattcatga acatgatgaa
     2881 agcgacaaaa gaggtcgatt ctattgattc tcaagaaaaa ttaggcgaag tgatcgagca
     2941 gggaacagcg ggtattcaaa gccgaggaaa ggcagaagct gacgataaaa cgatgttaga
     3001 tgtttggctg ccagtcacag aggcattgaa ggcagggaat ttaacaaaag aaatcattga
     3061 ccaggcaaaa gaacacacca aagtcttggt agctaaaaaa ggacgagctt cctatctagg
     3121 agagcgtgca atcggacata tcgacccagg agctgcttct agtgccattt tattttcaac
     3181 gcttttagat gtgatcaatc aaccttaagt tcaataaa
//
LOCUS       DAHEYX010000215         3142 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHEYX010000215 DAHEYX010000000
VERSION     DAHEYX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.217"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(87..584)
                     /locus_tag="MB219_002424"
     CDS             complement(87..584)
                     /locus_tag="MB219_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCC8428118.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(714..1424)
                     /gene="budA"
                     /locus_tag="MB219_002425"
     CDS             complement(714..1424)
                     /gene="budA"
                     /locus_tag="MB219_002425"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCC8428119.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1437..3101)
                     /gene="alsS"
                     /locus_tag="MB219_002426"
     CDS             complement(1437..3101)
                     /gene="alsS"
                     /locus_tag="MB219_002426"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCC8428120.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          861 a          707 c          517 g         1057 t
ORIGIN      
        1 aaaaaaaccg aaaaacagaa atgcccactg tctttccggc tctttttatt cttgatttct
       61 cttctttttt gtttcaatag acgattttag ttattaataa atgaaaaagc atttatatta
      121 aaatttgcta ataaaagatc tacatatttt tctagaaatg cttgatcgat ttcatcatac
      181 cctttcgtta ctccgcaatc gatatctaaa acaccgatta aattaccttc ttttaccatt
      241 ggcactacga tttctgattg tgcggctgaa tcgcaggaaa tgtaatttgc atgggttttg
      301 acatcatcaa cgatcaatgt ttcttgtttt tcagctgatt ctccacaaac tccttttccc
      361 atcttgatac gtgtgcaaga aactcgccct tggaacggtc ctaaaatcaa ttcattacca
      421 tcgaataaat aatatcctgc aaagacagtc tcaggcaaag tctcattcaa caatgcagat
      481 gaatttgcta aatttgctaa ccagttatct tctgcttcta ataatccttg ttgctgtaat
      541 aataaaattt cgtatgcatc ttttttctgt ttttctgtcc acatgataaa aaccgctcct
      601 ttttgatcta tggagaagat tatagctcag tttttttgta aacgctaggt ttcctcctca
      661 aaaaaacgag atacgtcagg ttaatcacct tgaacgcatc tcgctgtctt tttctattct
      721 gcttgttgga tttctgctgc taaatcagca taatcaatcg tttcgtgaac aaaagtcgga
      781 tcatcaactg gaaaatgctg tacaagggac gagatgcggc taatctcaac ttttccttca
      841 gccacttcaa aatccattac atgtccgcca aatgtatgtg tatcatcgat aaaatgcaag
      901 tgaaatcccg ctgcagcaac tccttggaat aattcaggcg taaagaaccc gactaatgtt
      961 ccttggacat tgctttgttg gaattctggt tgagactctg aaatttttgc tagtcttctg
     1021 taaggttttt cttgcttcgg catgatacgg atgtgcatat ttttgaataa tccagttatc
     1081 ttcactgcca aaaataggtt ttccccggct tctcttacta atatgtcttt tttcaattgt
     1141 tctccatctg cagtttttga gacagaaaaa ctagtatctg gcgtaaaatc tgtaattgct
     1201 gcataggggg taagttcact tccatctaat tgggcaactg agccatctga tcttccttga
     1261 taagctacgc catctaaaaa gatgacttca ccatcaagcc catgcaatgt tcccaatccc
     1321 agagttcctt tttctaatat atgagcaatc gtttctgttc catccatcaa gccagccatc
     1381 aaagctccta atgtcccgtg ctggtataag attttttcag tcatgtcgac acttccttaa
     1441 tagaattgat ctggtaagat tgtttctccc aatttctcat tatcacggta atcaatcgga
     1501 atatcaataa ttacgggtcc ttctgtcgca aatccttctt ctaaagcttt ttctaattcc
     1561 tctacacttg tagctcggat tcctttagcg ccaaaagctt ctgcatattt cacaaaatca
     1621 actggaccaa agtctacacc tgacgcccgc tgatatttca ttttttcttg gaactctacc
     1681 atattgtagt gtccatcatt ccaaatcaaa tgaacgatat tcaatttttt acgtaccgcc
     1741 gtttctaaat cctgggcaga gaataaaaat cccccatcgc cggaaactga tacgatttgt
     1801 gtatttggac gcaccaatgc agcagaaatc gcccatggca atgcaacacc aagcgtttgc
     1861 ataccattac tgaataacaa atgacgaggt tcataacttc taaagtggcg tgccatccaa
     1921 atataatgac tacctacatc tacggtaaca gtcatatcat ctgttacttt tgactgaagc
     1981 gtgttaataa cttctaaagg atgcaagata cctgcttctc cttttgattg aacaatgtcc
     2041 cgctccgtta attctgcttg aagagatgct aaatattctt ttgcatcttc tgacacttgt
     2101 tgaggctcta atgaacctgt caataaatca agagtggctg agatatcacc gatcaattct
     2161 ctttcaggct gcataaaagg atcgatttca gctggcgctt catcgatcac aatgatccgc
     2221 gcatcttttt ccgcattcca gtttcttgct tcatattcga ttggatcata gccaatagca
     2281 ataactagat cacttcgttt caaaagcata tctcctggtt ggtttctaaa taaacccaca
     2341 cgaccaaaga aatgcgcttc taattcacgt gagatcactc cagctgcttg gaatgtctct
     2401 acaacaggca atgctgtctt tccaactaat tgcctgatgg ctaacgtttc tttttcactt
     2461 gatcctcgca tacctactaa tagtaccggt agttttgctt ctctaatgcg ttcagctagg
     2521 taacggatat catctgcaga agcagaacct aatttaggat cgctcaatgg cttgatgaca
     2581 ttcccttgga caggtgcatc cactacatct tgcgggatac tgataaagct cgcgcctttt
     2641 tttgaactct tagccatacg ataagcattg gcaatcactt ctgaaattgt ttctggatct
     2701 tggatttctg cactgtattt tgtgattggc tggaacagtg ctgcattatc catgctttga
     2761 tgggtcaact tcaatagatc gcttcgtttt acttgtccgg caattgctaa aactgggtca
     2821 ccttctgctg tcgctgttac caaacctgta gctaagttac tagcaccagg accacttgtt
     2881 gcgataacta ctcctggctc tcctgtaata cgtccgattg cttgtgccat aaatgcagca
     2941 ttttgttcgt gacgagtcac gattaattca ggaccttgat cctctaattc attaaataca
     3001 ccatcgattt ttgcacctgg tataccgaat acataatcaa catgatgatt aatcaaactt
     3061 tctactacag atttcgaacc ttgtttcatt ttttcgtcca ttgaacatcg tcctttttat
     3121 gctttatttt taattattag tt
//
LOCUS       DAHEYX010000216         3127 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHEYX010000216 DAHEYX010000000
VERSION     DAHEYX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.218"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..333
                     /locus_tag="MB219_002427"
     CDS             <1..333
                     /locus_tag="MB219_002427"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCC8428121.1"
                     /translation="FYKDKKAIVIEGPLVRTFRHCLFCKESAKQIEKNGKKISMIILN
                     RSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCFISKQVHYTILEELTERQALKT
                     IAKRCDFQ"
     gene            490..747
                     /locus_tag="MB219_002428"
     CDS             490..747
                     /locus_tag="MB219_002428"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428122.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            834..2072
                     /gene="istA"
                     /locus_tag="MB219_002429"
     CDS             834..2072
                     /gene="istA"
                     /locus_tag="MB219_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCC8428123.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2062..2802
                     /gene="istB"
                     /locus_tag="MB219_002430"
     CDS             2062..2802
                     /gene="istB"
                     /locus_tag="MB219_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCC8428124.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT          991 a          644 c          630 g          862 t
ORIGIN      
        1 ttttataagg acaagaaagc catcgtaatc gaaggtccat tagttcgcac ttttagacat
       61 tgtctatttt gtaaagaatc tgcaaagcag atcgagaaaa acggcaagaa gatttcaatg
      121 attatcttaa atcgcagtgg gaataaacaa acctatttgc gtcttagaaa acaacattac
      181 cactgccaat catgcgggaa atattttact gctcatacgt atcttgtcgc tccttcatgt
      241 tttatttcga aacaagttca ttacacgata ttagaagagt tgaccgagcg tcaagcatta
      301 aagacaattg cgaaacgttg tgattttcag taacgacagt tcaacggaca ttatctgctc
      361 tatcggcaac gctgcgacag aagctagact atttacctca ttgtttacta ttcgacgagt
      421 ttcgctctct ttcaattttc catgggaagt tctccttttc ttgtatgaat tgagagacag
      481 ggaagctctt tgacattttg tccagccgga gaaaaaaaag atttgattgc ttatttcatg
      541 cgttttacaa gaaaagctca tatgggggtt cgatacattg tcacagatat gaatgcccct
      601 tatttttcat tagtcaaaga acccttccca aatgcacaaa tcatcattga ttgctttcac
      661 attgggcaac acttgaacaa gtttttcgat cgtgttcgaa aacgtgtgat gaagcagttg
      721 aatcaaaaat gtcaacggca cctttaaaat gtaggaaaac ggcaatttaa aattgttggt
      781 ttacctacat ttttgtatgc tcttcttatc ccaaatgaaa tggaggctca gggatgagaa
      841 aagacatacg cgaaggagtg atgatctacg tgatcaatga aatcaaaccc aattatgctg
      901 cactagcaaa acaatacgac tgcgactacc gaacagttaa gcatgcttac gaagaagccc
      961 aagttaaaga atcaaaacct ccagaacgta agaagcgtcc aagtaagtta gatccctaca
     1021 gagaaatcat ccaagacaaa atcaaagacc aatgcagtgc ttacagcatt ttcaagttta
     1081 ttgagcacaa aggatttgga ggaagctaca gtttagtaaa gacctattgt cgccagatta
     1141 agcaggcgaa agtacacaag gcaaccgtcc gagtcgaaca tagccctggg ctttctgctc
     1201 aggttgactg gaaggaagat atgcgactaa tcagtaatca gggcgaaatt tttgagttca
     1261 atatcttttt gtacgtcttg ccatattcca agaaaaaata tgtcacacta actttcgacc
     1321 gaaaacaaga cacactcttt gagtgcttag atgatgcgtt ttatcaaacc ggtggcgttc
     1381 ccaaagaaat ctggttcgat aatatgaaga cagtcgttga ccattctcgt agtcagtttt
     1441 ccaaaccgca tttcaacaaa cgtttttatg ctttcagtaa ggatgccggc ttcacaccaa
     1501 ttgcctgtcg gccatttcgt ccgcaaacaa agggctgtgt cgaagcttta gccagaactg
     1561 ttgaccgcct acgggtctat aatcatgaat tttatgatgc cgttgagctg tgtcatttag
     1621 tgaataacct ttgtgttgag ttaaataacg aggtttctca atcaactgat caaacaccag
     1681 atgaacgttg gcagacgaac gaaaaagagc acctgcatcc ataccaaaaa aaccttttga
     1741 atccctattt tgaagacttt attcgccgta aagtcaccaa ggaagaaatg gtcattttcc
     1801 gtaaatgcag gtactctgtg gcgcctcgct atatcggtaa agaagtcgaa atcgaactct
     1861 ccgataaaga agaccaagta caaatctatt ataacggaga actgattcgg tcgcatccat
     1921 taacgacaaa acagctgaac taccacgagg aagactattt ccaaatcttg aaatccgatg
     1981 tcatgagtca caaagaagac gatggaatca gagcgtatac tcgtgaaagt ctcaagcaat
     2041 atgatctggt ggaggttgcc gatgaagaat agccattacc aagaactgat gaccaactta
     2101 gagactcttg gtttgaatca tatgcgggaa tatgttccta actacatcga agtggccaat
     2161 cgagaggagc tttctttaac ggaagctctt ctcgagctga cacgcagaga agttgatcat
     2221 caaaataagc aaaaaatgaa tcgtgtggtg gcacgcgccc gcttttccaa gagaaccagc
     2281 ctggaagagt ttgattttgc cttccagcca agtatcaata agcgcgaagt gctagactta
     2341 aggcacatgg gctttgtgga aaaacatgag aacttggttt ttatcggcag tcctggtgta
     2401 ggcaagacac atctgacgat tgcattggga atcgaagcct gtcgtcaagg ctatcggaca
     2461 ctattcatca cctgtcatga gctgcttttg cgtctgcgaa cagcctatga aaaaggtacc
     2521 ctagagcgtg ttctcaaacg atacgctcgc tatgacgtct tgattattga tgagattggc
     2581 tatcttccga ttcaaaaaca ggaagctgat ttattctttc agcttcttgc catgcgctat
     2641 gaacaccgct ctacgatcat tacgacgaac attgtcttat ctcgttgggg ggagttgttc
     2701 caaagttctg agatcgcagc tgccatcctt gaccgtttag tacatcacgt caaggtgttc
     2761 aagatcacag gcaagtctta tcgtatgaga gacaagatct aaagtgtcga ttgtcgacaa
     2821 ttactattgc cgaaaaccta catttttatc ctgcccaaaa cctacaattt taagctgccc
     2881 ttgacatcaa aaagacactt cacaagctaa gtgctatcga caattgaaac ctttgtataa
     2941 attacttcta taatcagatg acgagttgga ttatacaaca ttcaagaaat ggcagaattt
     3001 tcaatggcgc tatttaacag aaagtgaagt agttgattgt ctaccctcaa tctcttatga
     3061 acttaaaatg gcttagcagt attatcaaaa gctgcttaac aattatcaca agaaacagct
     3121 agctact
//
LOCUS       DAHEYX010000217         3124 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHEYX010000217 DAHEYX010000000
VERSION     DAHEYX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.219"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            107..676
                     /locus_tag="MB219_002431"
     CDS             107..676
                     /locus_tag="MB219_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8428125.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(854..1294)
                     /locus_tag="MB219_002432"
     CDS             complement(854..1294)
                     /locus_tag="MB219_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCC8428126.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1452..2216
                     /gene="map"
                     /locus_tag="MB219_002433"
     CDS             1452..2216
                     /gene="map"
                     /locus_tag="MB219_002433"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCC8428127.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2248..>3124
                     /locus_tag="MB219_002434"
     CDS             2248..>3124
                     /locus_tag="MB219_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCC8428128.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSR"
BASE COUNT          985 a          544 c          638 g          957 t
ORIGIN      
        1 gcttgtgaag aaaatcaaaa actaattttg catatcaaaa aaacatgtta caatgaaatt
       61 aatcaatggt ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa
      121 gacgattact agagaacaga ttttggcagc tgcttatgaa gtagctgcca ccgatggatt
      181 tacacgattt actgcgagaa acatcgcgaa caaaatgaat tgttccacgc aaccgattta
      241 tcttgagttt aaaaatatgg atgacttgag acaagctctt tttgagcaga tctatgatca
      301 tttacaatat gaagtgttca ctgttgagca tacagggaat acgattatcg atcttgcttt
      361 taattatatc cattttgcaa gaagagagaa gaagttgtat cgttcactgt acttagaaga
      421 atatggtgga ggaaaagaga tgcagaattt ctcttatact tactttgtga atacagtgaa
      481 acgcgatcca gcttatgtga atctttcaga agagcagatc gaatctttgt ataatgggac
      541 atggatcata gctactggga tcgcagcttt gatgtcttca agcatcatcc atccaactga
      601 tgaacagatt gaaaagatga tccaagacag tatagatgct atcttgaaat tcaaagaacc
      661 aatcgaaatc gattgaatat actcaaaagt tccacaaatc agtaggtaac aagagatgtg
      721 tcaaaataag gaaaagttcg tttttcttac agcgtatgca tttttaactc ccgaagaact
      781 cttccatgaa tgcaatgaat aaaagagcca tacagattga ataaatctgg atggctcttt
      841 tattgcaagt atattaaatt tttgcgacta gttctttagc gaaagcttct aaattttcga
      901 tatcttcttc ttcagcattc agatccactt taacattttc ggctcctttt gatgcgcctg
      961 tttttgcaaa agcagcatcg aaatcatcca ccgatttgca gaattcatcg taaaaagtat
     1021 caccagatcc gcagacaccg taaatttttc ccaatagatc caattcttgc aagtcttcat
     1081 aaaagtctac gatctcatca ggcagatcgc catcatcgta tgtataagta gctactacgc
     1141 aaatatctgc ctcctcgaat tcatctgcat ctacttgggt acattcgttg atttcgactt
     1201 cgatatccag attttcaaat gcttctgcaa caatatcggc gatttcttca gtgtttcctg
     1261 tcatgctagc atatacgatt tttgctaaag ccatgatgtg cctcctcgtt tataattatt
     1321 taacaattat gaattatacc aaaacatcat tgaaaagaaa ataaaaccta aagacacctg
     1381 gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag
     1441 gagacgaaaa tatgattact ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg
     1501 gagctttatt agcagatgta cataaaaatc tgcgtgattt tataaaacca ggaatcacaa
     1561 gttgggatat tgaggtattt gtcagaaact atatcgagag tcatggcgga attgctgcgc
     1621 aaattggctt tgaagggtat gaatatgcga cttgtatcag tataaatgat gaaatttgtc
     1681 atggattccc tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta
     1741 tcgatttgaa aggcggcatt tctgattctt gttgggccta tgtagttgga gaatctacac
     1801 ctgagatcga ccgattgatg gaagtaacga agaaagcttt gtacatcggt attgagcaag
     1861 cacaagtagg gaatcggatt ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg
     1921 aaaatctagc gatcgttcgt gattttattg gtcatggcgt tggtcctacg attcatgaag
     1981 aaccagcagt tcctcattac ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg
     2041 ttatcacgat cgaacctatg gttaataccg gaacttggaa aatgaaaatg gatcctaacg
     2101 gctggactgc gtatacaaga gacggcggac tttcttgcca atatgaacat acattggcta
     2161 ttactaaaga tggtccaaga attttgacat cacaaggaga agaaggcact tattgatcta
     2221 tccagtcaat aggtacggga gaggtacatg aaagtttgga acaaaataaa acaaaatgaa
     2281 aatctgatgc gctttattga gacgactcaa aagcgcatca ctgattcaga aatgagtacg
     2341 acctctgtcg tcgttgctta ttatttactc ttgtctttat ttccattgat tattgctttt
     2401 ggtaatattt taccgtttct ccacatcgat caagagactg tactgaccta tttacgagaa
     2461 gtgattcctg agaccgttta ccagtttatc ggtcctgcaa tcaaagacct tttgacacaa
     2521 agttcaggag gcttgttatc cgtttctgca ttagcggctt tatggtctgc aagtcaaagt
     2581 atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt
     2641 ttgatcgtcc gtttcttctc actaattgtc attgtcttgt tcatgattgc tattagtggg
     2701 gtaactatcg ttcttgggtt gggacagctg attttggatg ctattcagcc aatcgtacaa
     2761 atacccgtga actttatcga tcagttccaa actttgaagt ggccaatcac attggtagct
     2821 ttatttgtca tcatgttctt gatttattta attgtcccta atgctcagtt gaagttgaag
     2881 acgatcattc cgggcacgat cttcgctact gtcggctgga tgctgttatc acaagtgttt
     2941 agtatctatg ctaggtattt tgcaacgagg atcagcggtt atcagatcat aggaagtttt
     3001 attgtactca tgctttggct taattttgct gctacgatca tcattttggg cggaatcatc
     3061 aatgcagttg ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg agatccaagc
     3121 cgaa
//
LOCUS       DAHEYX010000218         3112 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHEYX010000218 DAHEYX010000000
VERSION     DAHEYX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.220"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            30..305
                     /locus_tag="MB219_002435"
     CDS             30..305
                     /locus_tag="MB219_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCC8428129.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            311..1078
                     /locus_tag="MB219_002436"
     CDS             311..1078
                     /locus_tag="MB219_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCC8428130.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1246..3075
                     /gene="typA"
                     /locus_tag="MB219_002437"
     CDS             1246..3075
                     /gene="typA"
                     /locus_tag="MB219_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCC8428131.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT         1088 a          527 c          672 g          825 t
ORIGIN      
        1 aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag
       61 attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga
      121 atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg
      181 tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt
      241 ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg
      301 ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga
      361 gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct
      421 agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga
      481 tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg
      541 aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa
      601 tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga
      661 cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga
      721 gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta
      781 tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg
      841 tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta
      901 tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg
      961 tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt
     1021 agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa
     1081 aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa
     1141 aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa
     1201 aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac
     1261 gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat
     1321 gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg
     1381 gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta
     1441 gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc
     1501 gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa
     1561 ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca
     1621 atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa
     1681 gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt
     1741 tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca
     1801 accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc
     1861 gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc
     1921 ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa
     1981 ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa
     2041 cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat
     2101 atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac
     2161 attgacgaac caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga
     2221 gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca
     2281 gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt
     2341 ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa
     2401 gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa
     2461 cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta
     2521 cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc
     2581 ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt
     2641 tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc
     2701 ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc
     2761 atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga
     2821 atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa
     2881 caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa
     2941 caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca
     3001 ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct
     3061 aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta ta
//
LOCUS       DAHEYX010000219         3108 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHEYX010000219 DAHEYX010000000
VERSION     DAHEYX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.221"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            26..517
                     /locus_tag="MB219_002438"
     CDS             26..517
                     /locus_tag="MB219_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCC8428132.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(541..2916)
                     /locus_tag="MB219_002439"
     CDS             complement(541..2916)
                     /locus_tag="MB219_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCC8428133.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
BASE COUNT          927 a          641 c          529 g         1011 t
ORIGIN      
        1 ttatatgtat atcaggaggg ttcctatggc aaaagatgct agcttcgaca ttgtatcaga
       61 agtcaataca gaagaagtaa aaaatgcgat tcagcaaaca acaaaagaat tgtccaatcg
      121 ctttgacttc aaggattcta cagtagaaat caaattagag aacagcaatc tagttgtatt
      181 gagcgatgat gatttcaaaa tcgaacagat caaagatgta ttatttggta agctgatcaa
      241 aagaaatgtg ccaatcaaaa atatccattt ttccgaatca gaacacgctc taggtggcaa
      301 agctcgacaa actgccgatt tggtcaacgg aatcgatcgt gaaaacgcga agaaaatcac
      361 tacagctatc aaaaatgaaa aactgaaagt aaaagcacaa atccaagaag atcagattag
      421 agtgacaggt aagaaccgtg atgatctaca agctgtgatt gctttattac gtaaacttga
      481 cttaccaatc gagttgcaat tcactaacta tcgttaatca aaaaacgagg ctgacgtctg
      541 tcattcctcg tttttttgat ttactgcttc ttttgataac caaaaacgct gtattccttc
      601 ttccaattcc ccaacacttc gtacttctct tgctgtttgc caatgttgtg ggaataaacg
      661 tcgataagaa aaactagaaa atcgttgatc tgccaataca acgattcctt gatccgccat
      721 gccacgaatc actcgaccac cagcttgcaa gactttgttc attccaggaa gctgataagc
      781 gtaagcaaat ccaaggtgtt ccttctcatc ataatatgat ttgatcagtt cctgctcatg
      841 attcatctgc ggcaaaccta cacctacgat catactgccg atcaatcggt cccctctcaa
      901 atcgattcct tctgaaaaaa tcccgccaag tacgcaaaat ccaatcaatg tttcagcggg
      961 atttgtttga aattttgtta aaaatcgttc gcgttcttcc tcattcatgt ccgtttcttg
     1021 gattagcaca ttgctgtcgg gatattgctg tctgaatgct tccaccgcaa gatctaaata
     1081 ttgataagaa ggaaagaaaa cgaaataatt cccgaccttt ccatcagcaa aagcatgaat
     1141 cgtctctact aattttgcca gactgtgttc cctttttcga taagtcgtct ctaggtaatt
     1201 tgcgatgaaa acttgctggt ttccttctgg gaaaggactc ggcagtttgt aagccagact
     1261 tttttgtccc cccagtgttt cctgataata agagagtggt gaaaaactag cagaaaaaag
     1321 aatactgctt tttcctttat ccaacgactg ctcaagaaac aagcttggat caatacaaaa
     1381 ctcttttact atcagatcgc gatacgtttt ttcgactgtt gtttcgtagt gatcatcata
     1441 aaattcactg accttcaaaa aatgaagcaa atcaaaataa taaggaagca attgttcttg
     1501 tgatgggtgc tcaggaaatt ctgccagcca ttctttaatt ttttcgctta attgataacc
     1561 tgcctttact aaagattcag ccggtgcttt ttgatgatga taatcccaat gatcttcttt
     1621 tgctatttct cggatcgaat caaattcttt taatagtttg ttgaaacggc gatgaagttt
     1681 tttgaattcc tttggtatag cttccttact tcgtttcgtt ttttcataag atagttctgc
     1741 cgaatacatc tctcgggaac gattcaccaa attatgtgct tcgtcaatca aaaacagata
     1801 gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa acagttggat caaataaata
     1861 attgtaatcc ccaatgatta cgtcacacca gatactgaca tctaatgata gttcgaaagg
     1921 acaaagtgta tgctttcttg cataagtttc gatgacttca cgagtgatct gattctcatg
     1981 attcaaaaga tcccataaac cttcattaat ccgattgtaa taaccattgg catatgggca
     2041 ctgatctgga ttacaggtcg tctcatctaa aaagcagatc ttgtctttcg ccgtcaaagt
     2101 gacacttttt acttcagctc ctgtatcttt taatgctgtc atagcatctt cagctacttg
     2161 tctagttatt gttttagccg ttagataaaa gatcttttct ccctcttctt cccctacagc
     2221 tttcaaagcg gggaataacg tagagattgt ttttcctgtt ccggtaggtg cttctacaaa
     2281 caatttctgt ttcgtacgga tcgttttata agcagctacc gctagttcac gctgaccttt
     2341 cctatactca tcatggggga atttcaaagc catcaaagag gcattgcgta cagtgcgcca
     2401 gttttcttga aaaacaagcc attctttata atcttcagtc agctttttaa aaaaagtttc
     2461 taactgctct cttgtctgat gttcaacctt tctcgtgatc acttcttctg ccgtctggaa
     2521 gtaagtcaat tgcagattta tttcttgtaa atcatgttct tgactataga tataggcata
     2581 cacccttgct tgatgaaaga aaagttccac ttgttctggt tctaaatctt caaatcgtgg
     2641 ttcggaggtt ttgatttcat ctatataata aataccgtct cttttgaaaa taccgtctgc
     2701 acggccttca acgatcaggt aatattcatc taattgaact gttgttttta aaaagacttc
     2761 tttttcatag ttttctcccg catctttttg gagcttgcgg tggattctcg ctccttctaa
     2821 tgcagtatgg ttactttttt tacgattgtc gatacttcca cgtcgcaaaa taaattctac
     2881 taagtgccgc acagatattt ttgactgttt gatcatttcc accacctctt tctctctaag
     2941 attacctgaa aaaacctagt ttagcaaaaa gagctctctt tccaaacata aaaaaacacc
     3001 ctgctgatta ggcagggata taggatatag aatttggatt tttggatgaa tctaatgtaa
     3061 caaggacata ttaaaatgct atgaaaactt actttccttc tctttaga
//
LOCUS       DAHEYX010000220         3027 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHEYX010000220 DAHEYX010000000
VERSION     DAHEYX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.223"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(162..1463)
                     /locus_tag="MB219_002440"
     CDS             complement(162..1463)
                     /locus_tag="MB219_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8428134.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(1475..2092)
                     /locus_tag="MB219_002441"
     CDS             complement(1475..2092)
                     /locus_tag="MB219_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428135.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            2133..2825
                     /locus_tag="MB219_002442"
     CDS             2133..2825
                     /locus_tag="MB219_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCC8428136.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          962 a          562 c          584 g          919 t
ORIGIN      
        1 ataaaggaag tagccttttg aacaagggcg cttcctttat ttgttgtggg acaccagagt
       61 atactttttt ataaaaaata ggttgatggg aaaccctaag atttcccatc aacactaaat
      121 agtatagaac catattttcg ttacaaggtt aagaatgacg actatagttt ttcatctact
      181 aattttgttt tattgctctc catgacttta aaaaatggat agtagatgaa gatgctgatg
      241 acgatcaaca gtaggcaaaa gagaatattg cgccagtcca tacttgaaag ataagcttgt
      301 gcaaaaaaag gtgtgaagga aggatcgacg ataaatccaa tcccaatcca tcctagttgc
      361 tgagcaaagt aggtcagcaa taatgaaatg atgggcgtaa tcaaaaatgg aattgccaaa
      421 atagggttaa aaacgatagg taatccgaaa atcactggtt cgttgatgga acagattcca
      481 ggaaccaatg acaattttcc aattgtgcga tattcgggta ctttgcttcg catcatcaat
      541 aaaaccaaac ccaaagtacc accggctcca ccaagtacag aaatacggaa catttgtaag
      601 ttcattaagt gtgtcatggt ttcaccattt gccagttgtt cagcattcaa accagtttga
      661 gccattccca atgtaaagac gatagggaaa ataattgacg agccattgat accaaatagc
      721 cataggatgt tacccaaagt cacgatcaat aagaaaccac ctagactgcc agcgatgttc
      781 gttgctggag tcaggacttc catcaccgct tcagggaaga ttttgtttgt tgctgccaag
      841 aaaatcagat tgatgccata aaagatgaat atatttgtca gcagtggaac gagcatattg
      901 ataaaggttg ctaccatggg tggaactgta tctgggagtt tcaacttaat attttttact
      961 tcaaatagtc ggttgatttc cactactagc aagccgatga taattgcgac gaacaatccg
     1021 tttgtcccaa ggaaattaag attgatggca ccttcttcca ctggtgtaca aaccattaga
     1081 taaatgacag atgcaaccat gccattcatg gaaggattaa ttttgtattc attggcaagg
     1141 gaaaatgcaa tgccaaaaac actaatgaat ccgatgattc ccattgtcaa attataaggg
     1201 gctgtgatga gatcataatt tgctacggca aaatctttcc agctggccat aaatttcatg
     1261 aaaatattag ctgtatctgg attgtattta tcaatattga tgggtggatt tgcaaaaatc
     1321 agaaaaaaag aaccgataac aataaagggt aggccaaaca tcataccgct ggatatagct
     1381 tttaggtggc gctgattacc tatcttggct gcgatggggc taagtatgtc attcagtttc
     1441 atgataaacg cagaattttc catataactt cctcctatac ttgccaagga ttaaatgaaa
     1501 acgtacttgg cacgacgtga tctggatcat aggtctcgat gattttttcg tattgttcct
     1561 tgtagtcatt atcaactttt gcttggggga tcactttact tgcttcatgc aaaaagtgtt
     1621 ctgaaccacg ccccattgcc tgtccccgaa cagtatgttt atccaacgca atatcaggaa
     1681 tttctggaat atatcccata gcgaaactct tgataacaat gttcttaagt aaatccgatg
     1741 aacgatcttt ttcagattgg cataggtaac gaatcgcgtg gaaaaaatac atcgcacgat
     1801 cggattcatt gtattggaat tctttgcgca tggcgtttaa gttattgatc atgatggcgg
     1861 ccataggatt gcccatgccc acatcctcaa cactgatcgc caatagcctt ctccagagtt
     1921 tctcttcaaa ttgtggtgag gtgatataca tttcataagc aaattcacat gcttcttttt
     1981 cttttcctcg acgaatcgat ttctgtaatg ctgagatagc ttcatctcca cgtaagccgt
     2041 tacgggtagt catttttgac cacggatctt gaataaattt cgtttctgtc ataatataat
     2101 actcctatta gataaaattt gaggtgaatc ttatggatgt tgaaatgctt atagataaat
     2161 accaactgaa tgactcagaa gctgaagtct tacgatttat gaaaaaaaat cgtcagtctt
     2221 taaaaaatat tgggattcgt gacgttgcca aagcatcgta tgtttctact gcaacgatta
     2281 tcaatatggc gaagaaaatt ggatattccg gttatagtga gttggtattt gctctaggaa
     2341 acagccaatc tgacattacg atgccaagca cattctccaa tgaggagaag gaagcattca
     2401 tacatttaat gtctgcgtat cgcgataaac ggattatgat tcttggctca ggtttttcgc
     2461 aaaatctagc caattatttt tcggaatctc ttaatctata tggatttcgt gcgacggcca
     2521 atagtcattt agaatttctt agacagcaag tgcaacaaga tattctgctt tttattattt
     2581 ccaattctgg tgatacagtt cgattagccg aattagctaa aattgcgaac gatcacttta
     2641 tcgatgtgat cgcatttgtt gggcaaaaaa attcaaaaat cggtacgctg gcaacattaa
     2701 ccattagcac ggaaacctat attccacaaa aaatatctga ttaccaacca aatcttttct
     2761 ttggcacgac actgaatcaa tttgagcttt tgttgagcgc ggcgctgaag gctatttata
     2821 aatagaagaa cgctgtggcc actgtttctt caaaatctag tataaagaat cttctttaga
     2881 atgtaattgt tttctgtgtg ttctgaacat gtttaggaat ttccaaactt gtttagaaaa
     2941 ctttctcatt ccctccgctg tagaattgaa ctcacataaa aaacatctaa accaaaatat
     3001 ctacaagctt ggaaaggata ataggtt
//
LOCUS       DAHEYX010000221         3027 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHEYX010000221 DAHEYX010000000
VERSION     DAHEYX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.224"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            165..959
                     /locus_tag="MB219_002443"
     CDS             165..959
                     /locus_tag="MB219_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCC8428137.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            949..2991
                     /locus_tag="MB219_002444"
     CDS             949..2991
                     /locus_tag="MB219_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8428138.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          979 a          484 c          610 g          954 t
ORIGIN      
        1 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
       61 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
      121 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
      181 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
      241 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
      301 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
      361 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
      421 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
      481 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
      541 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
      601 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
      661 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
      721 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
      781 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
      841 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
      901 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
      961 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
     1021 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
     1081 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
     1141 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
     1201 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
     1261 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
     1321 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
     1381 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
     1441 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
     1501 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
     1561 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
     1621 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
     1681 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
     1741 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
     1801 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
     1861 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
     1921 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
     1981 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
     2041 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
     2101 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
     2161 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
     2221 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
     2281 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
     2341 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
     2401 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
     2461 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
     2521 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
     2581 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
     2641 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
     2701 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
     2761 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
     2821 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
     2881 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
     2941 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
     3001 taagacaaga taataaatag aactaaa
//
LOCUS       DAHEYX010000222         3007 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHEYX010000222 DAHEYX010000000
VERSION     DAHEYX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.226"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(276..692)
                     /locus_tag="MB219_002445"
     CDS             complement(276..692)
                     /locus_tag="MB219_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428139.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="MB219_002446"
     CDS             complement(695..979)
                     /locus_tag="MB219_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428140.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="MB219_002447"
     CDS             complement(1679..1774)
                     /locus_tag="MB219_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCC8428141.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="MB219_002448"
     CDS             2427..>3007
                     /locus_tag="MB219_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCC8428142.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DAHEYX010000223         2945 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHEYX010000223 DAHEYX010000000
VERSION     DAHEYX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.227"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            6..977
                     /locus_tag="MB219_002449"
     CDS             6..977
                     /locus_tag="MB219_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCC8428143.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="MB219_002450"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="MB219_002450"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCC8428144.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="MB219_002451"
     CDS             2004..2612
                     /locus_tag="MB219_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCC8428145.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2678..2851)
                     /locus_tag="MB219_002452"
     CDS             complement(2678..2851)
                     /locus_tag="MB219_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428146.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          780 a          625 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctcc
//
LOCUS       DAHEYX010000224         2874 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHEYX010000224 DAHEYX010000000
VERSION     DAHEYX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.228"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            111..695
                     /locus_tag="MB219_002453"
     CDS             111..695
                     /locus_tag="MB219_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCC8428147.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1066..2391
                     /locus_tag="MB219_002454"
     CDS             1066..2391
                     /locus_tag="MB219_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCC8428148.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2384..2734
                     /locus_tag="MB219_002455"
     CDS             2384..2734
                     /locus_tag="MB219_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428149.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            2731..>2874
                     /locus_tag="MB219_002456"
     CDS             2731..>2874
                     /locus_tag="MB219_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428150.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYH"
BASE COUNT          994 a          427 c          604 g          849 t
ORIGIN      
        1 ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg ttcagttaaa
       61 ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag
      121 gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata
      181 aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag
      241 gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag
      301 atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg
      361 gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac
      421 agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg atcctggaac
      481 gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa
      541 aattgtctga acaagatttg gaattactga agactttagt ggatagcggt acacctatta
      601 agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa
      661 tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat gctgaaaatt
      721 tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt gtctactgat
      781 aacttattat caaatgttgt aaggatttta ttcatttgtt aatattaaag aacggaactc
      841 caaaaaagtc cgtactctaa tttgcagtct ctcactcttt tagagtgaga gtttttatta
      901 gattttcaaa ttaattagta aagtgataga ataaattgat catacaaatc ataagaagaa
      961 gcagggtgat agcataccta tgcttctttt tttatgtatt tagctatttc ttctttacaa
     1021 catcgaacat atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     1081 aaagagccag taaatgatta ctttttaatc gatatgaaaa gcttttacgc aagtgtcgaa
     1141 tgtatagaaa gaaatttaga tccattaaca acagaacttg ttgttatgag tcgatctgat
     1201 aatactggtt caggattgat attagcttct tctcctgaag caaaaaagcg gtatggtatt
     1261 acaaatgtga gtagaccacg tgacttacca caaccatttc ctaaaacact acacgttgtt
     1321 ccaccacgta tgaacctata tatcaagcga aatatgcagg taaataatat tttcagaaga
     1381 tatgtggctg atgaagatct actgatttac tcgatcgatg aatcgatttt aaaagtgaca
     1441 aaatcactga atctttttac gactgaagaa acacgaagtc agcgaaggaa gaagctcgct
     1501 caaatgatcc aagaacgtat taaagaagag ttaggtttga ttgctacggt aggcgtcggc
     1561 gataatcccc ttttggctaa attagcctta gacaatgaag caaagcataa tgaaggattt
     1621 attgcggaat ggacgtacga aaacgttcct gagaaagtat ggaatattcc cgaaatgacg
     1681 gacttttggg gaattggttc tcgaatgaaa aaaaggctca accaaatggg aattttgagc
     1741 attagagacc ttgctaactg gaatccttat acgattaaga atagattagg tgtgattgga
     1801 ttacagctgt acttccatgc taacggaatc gatcgaacag atatagcaat cccgccagaa
     1861 ccgacaaagg aaaaatctta tggtaattca caagtgttac cacgagatta tactagaaga
     1921 aatgaaatcg agctggtggt aaaagaaatg gcggaacaag tagccattcg tatcaggcag
     1981 cataattgta aaacaggatg tgttcattta aacattggta cgtcgatctt agaaacgaga
     2041 ccaggatttt cacaccagat gaaaatacct atcacagata atacaaagga attgcagaat
     2101 tattgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     2161 acctatagca agcttgtgta caccgattcg cttcagctgg atttatttag tgatccacaa
     2221 aagcaaatta acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     2281 tttacgtcga ttgtacatgc ttcttcaatg ttagaaagcg ctagaagcat tactagaagc
     2341 acacttgttg gtggtcatgc gggcggaaat gggggaatta aaaatgattg attacaatga
     2401 tcgtggacgt ttaaagtggg cagggtttta tttatctgat catactgaag aaattgatgc
     2461 cgacaatgaa caacgaacca gtcaaaattt agcaaaagaa caaatgacga ctgaagagat
     2521 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatctattc aaaaagaaga
     2581 acgaaacgct gaaggatact atccagaaga tattgttggc aagatcttag gtagtgatga
     2641 gctggggatc tacgttggaa aagacaaaat tcactacgac gaaatacgtc atatcgaatt
     2701 ttaccaggat aataaatgga gtcgattaga atgaacatta tccagcaata tgagctaaag
     2761 tatataacct ttgatcagct atcagaagag atttggggtt atggccaacg tttgataaat
     2821 gaggttggtg ttgagcgctt ctctttctat gtagaagctg cagcaggtta tcat
//
LOCUS       DAHEYX010000225         2860 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHEYX010000225 DAHEYX010000000
VERSION     DAHEYX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.229"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            28..987
                     /locus_tag="MB219_002457"
     CDS             28..987
                     /locus_tag="MB219_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCC8428151.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1120..1326
                     /locus_tag="MB219_002458"
     CDS             1120..1326
                     /locus_tag="MB219_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCC8428152.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            1337..2356
                     /locus_tag="MB219_002459"
     CDS             1337..2356
                     /locus_tag="MB219_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCC8428153.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2545..2618
                     /locus_tag="MB219_002460"
     tRNA            2545..2618
                     /locus_tag="MB219_002460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2579..2581,aa:Ile,seq:cat)
BASE COUNT          966 a          480 c          570 g          844 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
      901 attcctagaa aatcactgga ttatcgaaca cctttggaag tatttttgag ttacgtaagt
      961 attgatgatc tgtctaactt aatttgacaa ttaagaaaga taaaattgaa caactggacg
     1021 ggcaaattga tgccttgctt tcagaaagag aaatattgat agaagaaaac aagcgattga
     1081 aaaagaaagt agcccaacca atgaagggag gtggatagta tgaacaatag aacctttgat
     1141 attttgaaat ggatcgctat agtagttatc cccgctttag cgacttttgt cggtcttgtc
     1201 ggaaaaggtg ttaactggcg gtacacagat ataacggtga tgattattac aggttttgga
     1261 gcatttctag ggagtatttt aggtgtatca aatcgaacgt ataagaaaat ttcatcagat
     1321 aaataggagg atagatatga aaaagaaaat tactattact gcgatgagcc tgttaacggc
     1381 tcttttttta ttgccaatta acggatttgc ctatactatc aacaatgaat ttaatttggg
     1441 cgcaaatgaa ggtagctcac aagtagcaaa taatcagtat attttactgc atgaaacggc
     1501 taatgaaaca gcaacaggac gcaatgaagc gcagtatatg caacgttcat ggactagcgc
     1561 ttatactgct tatattgtgg gagacggcgg aattgtttat caagtcggtc aacctggtta
     1621 tgtacagtac ggtgctggtt cgtatgctaa tgccaacagt cctgtgcaga ttgagttaca
     1681 acacacacat gataaagcaa cgtttgagaa aaactacaag gcatacgttg aattggctag
     1741 agattcagca atgaaatatg gtattccatt aacgttggac actccttata accaaccggg
     1801 aatcaaatcg catttatggg taacacaaaa catctggggc gatcatacag atccttacgg
     1861 ttatctttct gaaatgggcg taagtaaaga aaaattagca tatgatttag ctcatggatt
     1921 taccgatgaa aatccaacaa cttctgaaaa caagcctgtc attgatccaa cacgagctgg
     1981 tgcagctaat cctacactga cagatggaac gaattacgcc cacattgatc agtttggaga
     2041 aatcgaaaat gcaaatttgc atgtagctgg atggcacatt gctaactata aatacgagta
     2101 tatcttcatt atggattaca atactgggaa agaattagct cgagtaagag ctgatggaat
     2161 ttatagatca gatgtaaatc aagcttataa tacttctgga aatgttggct atcatgtatc
     2221 ttttaacatg cgtaacttcc ctaataagaa agtctatgtc atgatgcggg caacgaatga
     2281 tccagaggga aacactaaag gcggtgcgca agatttccat gacaaacgtt ggtatttaaa
     2341 tattcctaaa cgataaaaat agctcctcgt tgaggagcat tacataacta tattgacaac
     2401 tataaaaatc attcgataaa atagtgatgt tatcgcatat cttcactatc acccataata
     2461 gtcacactcc aagctatgcg ataacaggtt tgttgccaca cattctactg gttgattgtt
     2521 tatggcttta tgtggcaaca accagtaccc ttagctcagt tggtcagagc agacggctca
     2581 taaccgtccg gtcgtaggtt cgagtcctac agggtacatt aacgtagcca tttgaatcgt
     2641 tctgtgttag aattttttga agagtattat acaagctaaa gcttttcttc attgccactc
     2701 aaatgagtgg cttttttatg tatcctttta tggattaatg aaaggatgtt tcacatagtt
     2761 atatttctgt atatttgaaa agttttactt tgatttttaa atagaaagac atttgggtta
     2821 aattgtgaga tgataataaa gaagagttta aagcgcaccc
//
LOCUS       DAHEYX010000226         2854 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHEYX010000226 DAHEYX010000000
VERSION     DAHEYX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.230"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(121..483)
                     /locus_tag="MB219_002461"
     CDS             complement(121..483)
                     /locus_tag="MB219_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428154.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(557..937)
                     /locus_tag="MB219_002462"
     CDS             complement(557..937)
                     /locus_tag="MB219_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCC8428155.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1001..2194
                     /locus_tag="MB219_002463"
     CDS             1001..2194
                     /locus_tag="MB219_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCC8428156.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2271..>2854)
                     /locus_tag="MB219_002464"
     CDS             complement(2271..>2854)
                     /locus_tag="MB219_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCC8428157.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          889 a          554 c          482 g          929 t
ORIGIN      
        1 gaaaataaaa tgcactaaaa aactatcttt agcagaacat ttcattgtat tttttggacc
       61 aaacaataaa aaaagaagtt tgaaaaaaat tcaaacttct ttgttcgctt aatctgtatt
      121 ttagaagaaa ggcactccct ctacttcgta agttccaaga acagagcgca tttgacctgg
      181 ttcttcccaa atatatcctt cgcctaattc ttctctttga ttcttttgtt cagtttcaaa
      241 gacaaacaac ggaacatttt cctctttaat atgtgtattt gtcaattcca tcaaactaat
      301 cttgctaaca tcgatatgaa ctatttcttt taagaaatta agtatattcg ctaaaccagt
      361 ctttccttca gaaagttccg ctaacgcaaa ttccaacttg tcatcaattg aatggaccaa
      421 aaatttttta gtaccatcct ccaaatgcaa cataattgta ccagcaactc ttttgcttgc
      481 caaaatttcc acctcgatca acttgttcta tggcttacat aaaaattcta gcacattttc
      541 agaaaaaata cactttttat ttcttatttt ctttccgttc tagttcagat aatgcttctt
      601 ctatccaaat aggtaactgc tccgcaatag tggtaaatcc gatcttacga tttttattcg
      661 tgaaatacct ttttctgcga tatggaacag ctggatttgt tttttctaac gttcttaaag
      721 ataaactgat ttttttggaa tattcatcaa tgtcgatgat ctgaactgtt actggctgtc
      781 cgacttttaa caatgaatgg atatttttcg tataaccgga ttgtacttct gaaacatgaa
      841 taagtccttg cgtctcgtta tcaagagaaa cgaatgcgcc ataaggctgt atccctgtca
      901 cttttccttc aattatttgt ccaattttat aagtcatcta cttccctctc ttcgcattta
      961 tcattatact gtaaatagct caaaaaaaga agcgacgaaa atgactggag gtcttaagat
     1021 gatcgaattt gataaacaga tttcaagaaa agatacagaa agtgtaaaat gggacgcaat
     1081 tgctgagaca tatcaaatgg atgacttact tcctttgtgg gtagcagata tggacttctt
     1141 gtctcccggt ggggtagcta aagcgtttag tgaatacatc aaacatggaa tctttggtta
     1201 tgccactctg tctgataaac tatatcaggc tatcattcat tgggaacgcc aacaccatgc
     1261 tgtggagttg acgaaagaaa atatcgtttt tacaagtggt gtgttaacca gtttagctgc
     1321 agcagtacaa acttttacaa aaccaggcga ttctattttg attcatgacc cagtttatcc
     1381 tccattttcc tctattatag aaacaaatca acgtcacatc gttcgaagct ctttgctaga
     1441 aaaaaataat catttcatca tggacttgtc tgacatggag aaaaaaatcg aagaaaacaa
     1501 tgtaaaagca atgattttat gcaatcctca caatcctgga gggcgtgttt ggagcaaaga
     1561 agaattgaaa cagttaagcg aattgtgttt aaaacatcaa gtgattctgt ttagtgacga
     1621 gatccatcaa gatttaactt tattcgatca tacatttact tccatgctaa cgattgatcc
     1681 taatttagat gatctactag ttgcttttac ttccgcaacc aagactttca atctagctgc
     1741 aatcaaaaat tcaatggtat ttatcaaaaa tccagagatg aaagctgatt ttgaaaaaca
     1801 tttagtgatg aaccaacagc acgaaatcaa cacttttgga ctgattggaa cccaagcggc
     1861 ttatgagacg ggtggagaat ggttaagcca actgattcct tatctagaaa aaaatgttca
     1921 aacagttaat cagttttttg aacagtatct tccgaaagtg agaataatga accctgaagg
     1981 aacttactta atgtggttag attttaccgc ctatatttca gacgaccaag aattggagaa
     2041 cactcttgtt cggaaaggaa aagtcgtatt aaatcctggc atcacgtttg gtccttccgg
     2101 tcatgggcat atgcgcttga atattgcttg tccagaagaa actttactgg aaggacttaa
     2161 tcgaatcaag tatgctttag agacaatcga ttaaatcatt tattcaaaat aaaaggacga
     2221 gaccttacta ggtttcgtcc tttttcataa ttactccgta ccataccaaa ctattctgaa
     2281 atttcaattg tttcaatcac tacatcatgt acaggtcggt cttgtggacc acgttgtaca
     2341 tttgcgattt catctactgt gtccatccct tctagtacat gtccaaagac tgtatgtcgg
     2401 aaatctagcc aaggagttcc gccaccttta tacgcttcaa tgatttctga tgggaatcca
     2461 gcaccttcta gttgtccaag catattagct ggcacatttg tgttatttac aataaagaat
     2521 tggcttccgt ttgtgtttgg acctgaattt gccatagata aagctccgcg aaggttgaac
     2581 agttcacgac tgaattcatc ttcaaaactt tcgccataaa tactttcgcc acccattcct
     2641 gttccagttg gatcgccgcc ttggatcatg aaatcaggaa taacgcggtg aaaaatcacg
     2701 ccatcataat aacctttttt ggaaagttca atgaaatttt gaaccgtctt cggtgctagt
     2761 tcagggaata attgaacagt aatgtcacca cggtttgttt tgataacagc ttttggtcct
     2821 ttttcacttt ttaaatctag ttgtgggaat gcca
//
LOCUS       DAHEYX010000227         2803 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHEYX010000227 DAHEYX010000000
VERSION     DAHEYX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.231"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            535..>2803
                     /gene="esp"
                     /locus_tag="MB219_002465"
     CDS             535..>2803
                     /gene="esp"
                     /locus_tag="MB219_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCC8428158.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1012 a          420 c          548 g          823 t
ORIGIN      
        1 acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa ctccaatcat
       61 tcgtttaaga ttctaggaat cattcaggaa tgctgtgaaa ccgatacttt ataattataa
      121 agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact tatgtcacag
      181 tctcattcag taatgcggga taaggctcct tattccgcta tttgaatact ctgaagttta
      241 atctaagaaa gagtaatatt ttgaaaacat gcttcatcgc atagaaatga acttaatttc
      301 agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt aaaaagcaca
      361 aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgtgca tgaaattctt
      421 taagtaaact agttaacata agtttagtaa gattcctatt ctgtgaataa tacgtatcag
      481 atttatcttt tattcttact aagaaaataa ctattaaggg agttgatttg aataatggtt
      541 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
      601 atcaaaaaat taagtgttgg ggttgcatct gttttggttg gtgttggtct tgtcttggga
      661 acaactgaac tagttcaagc acaagatgaa atttctccaa gcaccccact tgaaacagcg
      721 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
      781 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
      841 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
      901 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
      961 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
     1021 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
     1081 ggattatttg ctggggctga aacaactgat acacaaactg ctcctaatgg ggtaattacg
     1141 tttgaaaaga gcttagatga aatttttaaa gagataggca tagacaatct caagaccgat
     1201 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccaggt
     1261 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
     1321 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgaggaacct
     1381 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
     1441 cataagttaa cgaaaaatta taatttctca tattcagctt ccgcaaaggg cacgccttgg
     1501 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
     1561 atggttcagg ctgacaaagt aacatatgat gtttcttttg aatcagggaa aaaagtggct
     1621 gatttagcaa ttgaacggcg caaagatcat gaaaattatg gtgtaggttc aattactgat
     1681 aatgatctta ccaaacttat tgattttgct aatgcaagtc cacgtccagt cgttattcga
     1741 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
     1801 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
     1861 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
     1921 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
     1981 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
     2041 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
     2101 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
     2161 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
     2221 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
     2281 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
     2341 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
     2401 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
     2461 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
     2521 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
     2581 ccagatgttc ctttagctaa agatgccttt tcaattttag atgtctataa taaggacttc
     2641 ggtaatgcaa gtgttgatgc aaatactggt attgttacat tcactccagc taaaggtgta
     2701 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
     2761 gtaggcacga ccgatttagc agtaactgta agtaaagata ttt
//
LOCUS       DAHEYX010000228         2785 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHEYX010000228 DAHEYX010000000
VERSION     DAHEYX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.232"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            43..303
                     /locus_tag="MB219_002466"
     CDS             43..303
                     /locus_tag="MB219_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCC8428159.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(1302..2537)
                     /locus_tag="MB219_002467"
     CDS             complement(1302..2537)
                     /locus_tag="MB219_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCC8428160.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT          832 a          451 c          570 g          932 t
ORIGIN      
        1 agtccaaact gtctaagttt ggtttttaga aggtgggaaa caatgtattg gctgatttta
       61 ttttttgttt ttatcttttt attaacggct agtcacctta tattgaatat gctagctaca
      121 tatcatattc aaatcaatcg atggatttgg gcactggcat catttttgat cgtgattctt
      181 ccaaagatca ttgtaccgca tatgaatgtt ttgttttcat gggggacata tgtactttgt
      241 ggaatttttg ctatcaattt tatgatcgag cagcatagat ggtttgtcac atcgaagctt
      301 taaatgttct aaaagtggtt gtgacagaag tggttagctt caagaaataa gaagaaattt
      361 acgaaaattg cttcttaaat ttttgtgaaa tgttggctta tttttcgttt ggttgaactt
      421 attaccgcag atgcaagctt ttgaccaccg tttattcgga atatgggact gtgacaaggc
      481 ttttgtcaca gccctttttt acataaagat tattattgtt ttaactgttt tggcagaaag
      541 aaggaggcaa aaatgaaaat tttatcagca atggattaag aattagtcga tctactacca
      601 aagtgatcaa ggcaaatata gatttgggag cttacaacca tatagtaggg gctgcagtat
      661 ggtgtatgaa tggtaatatg tatgatgaga gtgtttattc aaatcatgaa acgtgtatag
      721 aaataattgc gctgggaata gcaatcagtc atggtgaacg tgacttttag tttatcgttg
      781 ctgacgacat agaacattct gacatgatct actcagcatg tggggattgc tggcagttta
      841 tataggagta tgcatcggat tgcgagatca ttgtctcaac agaagaggga ggttgattat
      901 caaagataaa gtggtaaatc ctacatttga tttagtaatt gttgaaggga ctagcagcac
      961 aaatctctac aaagcgatgc ttctagatct atatgacaca ttttaagaaa atgtatttgc
     1021 ctaattatta tggtctttct tttgaagaaa cgttgagatg atccaaccag agaaaggtgt
     1081 caaagaattt gactaagaat aaatggcaga cgctacctat gctcatttgg attgcgtcag
     1141 atcttttatt ttaaggatga ttttgtgttt tttagacacc gtaatttaag taactctaac
     1201 ttttcaatcc actatttact ttttttgtat tttcactgtc tgggtctcga atagcgaaat
     1261 tactgatgag tctctattaa aaaagctttt tctacttgaa actatattgg gtggttgctc
     1321 aggaaggttt cgatttcttg aattgcttgt tgtggaccat ttattggaaa aacggcttgc
     1381 agcgtgcgtg cctgctgacg aatcttgggt gaagaaactt cgcgcagagc aatctgcagt
     1441 tttctttcag tcactggttt tgtgagggcg attgcgtttc cgtattttac acaatagtca
     1501 atattacagc tttgctcaaa ttgcatcccc acaccgataa acggtttgcc ggattcacag
     1561 gcagtttgga cggtcccttc cccgccgtgg ataaaagaaa aatcaatgaa ttcatgcaaa
     1621 agatgactag acagatattc tgttattaaa agatcttctg agtcagattg aatgtcatca
     1681 agcagatagt caataggtgc aataaccgct agatccggtt gtttttgtaa gaagaacaaa
     1741 attttgcgta acatacgcgg attgcctgaa ctacccattg cgaaatagat aatacgtttt
     1801 ctttcttgtt gctttcgctg tataaaagtt agcacttctt caggcaagga agtactgagc
     1861 ttagcaaaaa ttggaccaac gattcgataa ttttccggca gctgttcttc ttccaaaaat
     1921 agtttaggcg ttgtgatgag atttagatca gcatccatca tttgtaaact tgtttttggt
     1981 aaaggaagca tttctttttg tgcagcgtgt ttgaaggcat tggggatcca tcgggtattt
     2041 actatacgct tttttgtcca gtgccaaagg ggattgctta aagggccgaa gaaacgtaac
     2101 tcgtggggca tcagcgcatg ctctgattga aaaaaaggtt cactcaaagc atagggttta
     2161 acatatacta aaggaatctt tttattgcgg gccgataaaa aaattgtcac attcgaacct
     2221 gtcacaatca gtcgaggctc aaacttagca atcagactct gttcactagt gatacgagag
     2281 gcaaccattt ctttggtaaa agggttttta agtgtacgca actggtcaaa ttttatgatt
     2341 tgttcttctt gttttttcgt aaggatcggc gctagtaaat gatactcaaa gccactttct
     2401 gtgatcaaag gagcaaaagt tttagaaaag ccaaagaata cacagtaata acggtctttc
     2461 atagctttgg caacctcaat cattcgtgtt gtctcagcta aattgaatac tgcaggagca
     2521 aaaagaattg tcttcatgaa atcctcctta ttggaatgag tctatccctt cgtttcttac
     2581 tagtatacta aaaaacacag cgttcgagta gcctatgtat attttgaata ttaaactgtc
     2641 tctcgctgtt ttcacaactt ctaaatttaa attttgtaat caggttgaaa ctccaatttt
     2701 caaaaaaaca cgcaacaatt ttttgatcga tttatatgtt gtgccagata aatacgagaa
     2761 gttgtgtcat taaaaataaa catga
//
LOCUS       DAHEYX010000229         2771 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHEYX010000229 DAHEYX010000000
VERSION     DAHEYX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.233"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            172..516
                     /locus_tag="MB219_002468"
     CDS             172..516
                     /locus_tag="MB219_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCC8428161.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="MB219_002469"
     CDS             517..936
                     /locus_tag="MB219_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCC8428162.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="MB219_002470"
     CDS             complement(1028..1378)
                     /locus_tag="MB219_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428163.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="MB219_002471"
     CDS             complement(1344..2675)
                     /locus_tag="MB219_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCC8428164.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1031 a          500 c          533 g          707 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc a
//
LOCUS       DAHEYX010000230         2768 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHEYX010000230 DAHEYX010000000
VERSION     DAHEYX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.234"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            74..931
                     /locus_tag="MB219_002472"
     CDS             74..931
                     /locus_tag="MB219_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCC8428165.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            932..1480
                     /locus_tag="MB219_002473"
     CDS             932..1480
                     /locus_tag="MB219_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428166.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1777..2724)
                     /locus_tag="MB219_002474"
     CDS             complement(1777..2724)
                     /locus_tag="MB219_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCC8428167.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT         1030 a          414 c          515 g          809 t
ORIGIN      
        1 tttggtagta actaaaaata aatatgttat aatagtatca aatgaatatt gctaaaagaa
       61 aagaggtagg aacgtggtag cgaagaaata tgtaattggt aattttaaag gtggcgtagg
      121 taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca gttaatgaag gaaaaagaac
      181 cttgataatt gacttggata tgcaaggaaa tacttctgat gtgatgaact taacgcatat
      241 gaacttcact aacgaagaag gaggcggaga aggtgaaccg attgtttacg aaaatactgt
      301 gactgacgtg ttgataaaca atcttccacc taaagaggct gtctataaag taatcaataa
      361 cctctatata cttccagcag atatgtcctt tgaaatgtat gatgattgga tcaaagacca
      421 atataatgat tcattatccc aattcaaata tatgaaagaa aagctagaac cattatttga
      481 tgagtttgac gtcatctatt tagatgtccc accttctatt tctgtatata gtaaatccgc
      541 gatgtacatt gcggactggg caatcgtcgt cttacagacc caagtaaaat cgatgagaaa
      601 tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa gaatttgata caaatttata
      661 tgttgctggt gttattcctt tcatgttgga aagcggtgac gcagtagatc aggaaatgta
      721 taaacaagcc caagagattt atggggaaca tttgattaaa aatgttgtac tcaaaaatgc
      781 gcgcttaaaa cgatacgacg gatcaggtat tacgatggag aagacaaaga aaggtctctt
      841 aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt attttgaaag aattggaaga
      901 acacgagaat tggtatgcgg aggtggagta attggctgac atcaaaggac ttgatatttt
      961 tgggaacaac aaaggactcg ccaaaataaa aaaagaagca gaagcaaaga aaaagcagca
     1021 agcaattaca ccatctgaaa ataagcaatt agaagtgcac caagttcaag aaataaaaac
     1081 ttcctccaat gttgaaagac agagtagagg aagaacaaaa aaagttggcg caccaatcag
     1141 aaagtttgat aaggtttatt ccaccaagca tcctttaaaa ctgtcgactt tgttaaatgc
     1201 gacttccaga gttttagtag aaaagtatga gactaatatg acaagagatg aattgcttag
     1261 aaaagcttta gatgagtata ttaaacagaa tcttacgcaa gaagataagc aagatttgtt
     1321 taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa tatccaattg ttccagaatt
     1381 agatgaccgt ggagaaataa taagaactac cagtcagata aaacaagata ccgaagactc
     1441 actgcgcaaa gggtggggaa tgaataataa agttagatag aacccccttt ttatagcata
     1501 aaaaagattt agaaggatat atgatcctat tgcatgagaa gtacaataaa attaataaga
     1561 agtaacatta acgaaaaaac gtggaaatta ttcagtgaat acaaagaaat taaatcttga
     1621 aaagtaatgg agtatggctt ggaaaagttc aaaaaatatc aagacaagct tctcaaaaaa
     1681 agggattttg atatccaata aaaagagtga atcattgtac tgccctccaa aaaagtagta
     1741 ccaaaaatct aaattttttg gaggatttta catgactcac tcttttttta tcaaagaaag
     1801 tattttccaa gcagtttttt catccaaaaa taaatctgta aaaaaattac cattcaaggc
     1861 gcctaaacaa ctctccgctt tgaacttact tttacaaata ccaattcgtt ttggaatatc
     1921 gaaaatttct tttaaagaaa gaccaaaagt cttatcctca gatgctttga tgaattcacc
     1981 attgatattg aagggtcttc cgtaaagaaa tccaacagta tccgtcatgc ttggaaatag
     2041 gattttacga tgcttgttcc agggttcgac agaatcaata gaggataagg tgccaattcc
     2101 agaaattaac atatctgcat gcgaagcggt tgctaaggta ttagctaaag aaggttctag
     2161 agccattagt tcacgtgcag tttcattgat gacatataaa ggaatactga gagtcaaata
     2221 agaagactca taacgttcag cagctttttg aactaatcgt gtggagccag ctaaggaatt
     2281 atattttccg atttcgccaa tgaattgagt aaaaattaat tcttcacgag cacccatttt
     2341 aaactgatca atcactttgt aaacggtatc tccccaagat agtgcaacca cattggcttt
     2401 cttaattaat tcttgaatgt aatcggcagc aaattttgaa aataataaat cgtgttgtga
     2461 taagtcttca ctacttttta atatatagat attcgtttta aagtaatttt tgaagatttc
     2521 ttctagctca taattcctag catacggaga attaattgaa atactaacta attgtttttc
     2581 acgtgcatct tcaatatatt ttaaagcttt atatctggtg atcccatatt ttttagaaat
     2641 ttcactaata ttgatatcct cgagataata gtcttcacaa atttttacaa ggagtgattc
     2701 atagtcagag aattccagat gcatagtata gctcctaact ttttcaaata gatatttttt
     2761 ctcattat
//
LOCUS       DAHEYX010000231         2737 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHEYX010000231 DAHEYX010000000
VERSION     DAHEYX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.235"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1481
                     /locus_tag="MB219_002475"
     CDS             <1..1481
                     /locus_tag="MB219_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428168.1"
                     /translation="FYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKY
                     FLFFTVFLVLCWLPYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVH
                     DLPTDTINHGVFLFSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPI
                     YALNMQKDTLFSIACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVV
                     TGTGLICGLIILKQKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQ
                     ISRTIVMDGDLSKKDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFL
                     SLWARNLPSNIKIYTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLT
                     GLSGMKKIIDARDFFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATP
                     VAFSLRYVFVLALGLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            1468..2670
                     /locus_tag="MB219_002476"
     CDS             1468..2670
                     /locus_tag="MB219_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428169.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT          775 a          486 c          469 g         1007 t
ORIGIN      
        1 ttttttactt tcttgtttat ttctccattt ttgtgtattc ttttttattt ttacaatcga
       61 tttatttatc aatgccaaaa ctggaaacat tttttactgc tcaaaacaca caggctttct
      121 cttcaagaaa atatttttta ttctttactg tctttttagt tctgtgttgg cttccttatc
      181 tgttggcatt gtatcctgga gtggttttgc ctgattcgtt aagttcggtc gcccaatcta
      241 tgggtgatgt tcctataagc aatcatcatc cagtcttatt caccttactg gtcaagctat
      301 ttgttcatga tttaccaact gatacgatca atcacggtgt atttttattt tcgatttttc
      361 aaacattgat aacagcttca gcgataagtt attctctttg ctggctgtta aaaagaaaca
      421 tccatttttt tccggtactc attagtttat gctattttgc atttgcaccg gtttttccaa
      481 tctacgcctt gaacatgcaa aaagatactt tgttctctat cgcttgcttt ttattttcct
      541 tagcggtttt tgactttatt tttcaacgag aaaagccaac gattctcaat cagatttacg
      601 taatcatcct tgctttactg accacctatc taagaaataa tgggatttat gtagtaaccg
      661 gcacaggttt gatttgcggc ttgattattt tgaaacaaaa aaggtatcaa gcattctttc
      721 aagtggtgct gggttataca gtgattgctg gactactcat ccacccgttg atggctcgtt
      781 atgctattcc tacagaaact gctgaacaat taggtgttcc tttgcagcaa atttctagaa
      841 ctattgttat ggatggtgac ttgtctaaga aagataaagc attcatgaat cagctactcc
      901 cgataaaaga ttatgaagcg tattctccta gtcttgcaga ctcaatcaag tggcataaaa
      961 attttaacaa tgagtttttg aatacacata aaaaagaatt tctatcacta tgggcaagaa
     1021 atttaccaag taatataaaa atctatacag atgcttatat tttagaaacg ttcgggttct
     1081 gggtacctgg tacaaaaaat agttacggct ttttagatac tcgtgtaaac acgaataatt
     1141 atggtatgaa gcaagtagat ttgattgaac atcttactgg attgtcagga atgaaaaaaa
     1201 tcatcgatgc tcgtgatttc tttggttccg gcacattact gtggttagct atcggcagtg
     1261 cacttttagc aatctttaag aaaaaatatc ggtatttact cctttttctt cccggtatgc
     1321 ttgtttttgc tactattatg attgctactc ctgtggcttt tagtttacgg tatgtgtttg
     1381 ttttagcatt aggtctacct gtttatatcc tctctccttt tttactagat aaaagagaaa
     1441 aaatgaaaac agatatagga gatactcatg aaactattta aaaagatatt ttttgctttt
     1501 gttctcttga tcgctagcta ttgcgtacta ctgacaatta cttttttcat tccgcagcac
     1561 tctattgaat cgaatgcaga aaaatctcta cagatggttg aaaaagaagg aatgtatcct
     1621 tctatcaatc aaggaaatat gctaggcact cggttagata attttactga tcatttgatg
     1681 atacgaaaaa caaaagcaga tcctgaattg aactccttgg agaacgcgat gtctatggcg
     1741 gattatcccc gttattggca tggttatcag ctttttttac gacctttact gctagggatg
     1801 agtttgggga gtattcgaat gatttacgca gccattcttt ttctgctgat tggattgacg
     1861 agctactttt taaccaagtg tagcgacatc tatttaacga cagccttgtt gatttcgctt
     1921 gctataggaa atgctgcaac ctttttcttc agtatgcaat tttcaaatat ttggattttg
     1981 actttattgt ctgtattact atttttaaca aaaccacaac tctccaacac caaccaacaa
     2041 ttattcttgt atttctttgt cattggttct ttagctaact tttttgactt gttgacaaca
     2101 ccggtcatct cttgggggat acctgtgatc attatttatt atgtaacaaa taagtattta
     2161 atggataaac gcagttcatt gagcaaacaa gtaggcagtt ttgtttttac cggtatcttt
     2221 tggggattag gctatggttt gacatgggtg gctaaatgga tcctatcatc gatcatattg
     2281 aacaaaaaca ttgtaaaaga tgcaatcaac caaatcctct ttcgaacgga aggaaacgac
     2341 aaatacccgt tgcatcgatt agagatgctg aaaagcaata ttagattgat gtatccaaaa
     2401 gtagcaatat tattgctatt gattacttgt cttgtttttc tctattttgc ttatcgtaaa
     2461 cgaaatagct ggcgtccgtt ccaacttcta gttttgtttg ttttagctgc tgtaacgccg
     2521 tatatttggt acaatatttt agctaatcac tcacagattc attattggtt tacgtatcgt
     2581 acgcaaatca tcacaagttt tgctatactt agtgctttcg cctttttgat ccctcctgag
     2641 aaatcaaaag acgaactaaa ttacttttaa gaataccgat gtactaattc atttttaaac
     2701 ataaaaactc cagctgtcag agatagccgg agttttt
//
LOCUS       DAHEYX010000232         2648 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHEYX010000232 DAHEYX010000000
VERSION     DAHEYX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.236"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(31..711)
                     /locus_tag="MB219_002477"
     CDS             complement(31..711)
                     /locus_tag="MB219_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCC8428170.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            682..1005
                     /locus_tag="MB219_002478"
                     /pseudo
     CDS             682..1005
                     /locus_tag="MB219_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1134..>2648
                     /locus_tag="MB219_002479"
     CDS             1134..>2648
                     /locus_tag="MB219_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCC8428171.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGNGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPEF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLA"
BASE COUNT          869 a          466 c          531 g          782 t
ORIGIN      
        1 aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat
       61 ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc
      121 tcgaatggct tccatgcctt taatcgtggt agaggcagtg cgtaaacttc gatagaattt
      181 attgcgtctc tttactggac gatggtcttg ttcaatcaaa ttattcaggt atttaatggt
      241 acgatgttct gtcccttgat aaaagccgta ttcttttagt ttcttaaagg cacttgtaat
      301 agagggggct ttatctgtga ctacaacctt cggttcatca aactgcttca ctaaccgctt
      361 aagaaaagca taggctgctt gtgtgtcccg ttttttacgt aaccaaatat ccaaggttaa
      421 accatctgca tcgatggctc gatacaaata atgccatttt cctttaattt tgatgtacgt
      481 ttcatccatt ttccatgaat aaaaggattt tttatttttc tttttccaaa tttgatagag
      541 tagtttgcca tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg
      601 atcatataag atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac
      661 ggctacaata atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc
      721 tcctgctatc tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca
      781 gaaccgccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
      841 gttttgaaag aatatgagcg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
      901 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
      961 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
     1021 cgaacgcact tagcagattc attgagcttt tcgtttctag ttaccggtaa caaggatact
     1081 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
     1141 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
     1201 atagcaacgg caatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
     1261 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
     1321 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
     1381 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
     1441 tcaatcatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
     1501 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
     1561 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
     1621 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
     1681 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
     1741 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
     1801 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
     1861 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
     1921 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
     1981 aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc gcttggcaaa
     2041 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
     2101 ccgtttcagt gtttggagat actcgtcccg agttttgggc tatgtctgca aaagcaatcg
     2161 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
     2221 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
     2281 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
     2341 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
     2401 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
     2461 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
     2521 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
     2581 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
     2641 cacttgct
//
LOCUS       DAHEYX010000233         2580 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHEYX010000233 DAHEYX010000000
VERSION     DAHEYX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.237"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..962)
                     /locus_tag="MB219_002480"
     CDS             complement(<1..962)
                     /locus_tag="MB219_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8428172.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1078..1713)
                     /locus_tag="MB219_002481"
     CDS             complement(1078..1713)
                     /locus_tag="MB219_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCC8428173.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(1796..2218)
                     /locus_tag="MB219_002482"
     CDS             complement(1796..2218)
                     /locus_tag="MB219_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCC8428174.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2236..2556)
                     /locus_tag="MB219_002483"
     CDS             complement(2236..2556)
                     /locus_tag="MB219_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428175.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          738 a          462 c          374 g         1006 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttaac ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagta
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgatgc tctaatatgg ttattaaact taataacggt ggattttaca
      601 tggtctttcg catacttatt taaaaagcgt tgatatccaa ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttcgccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ctactgtata
     1021 ggtttgtttt ttcatagaac acgcttgctt tggacggtgg gcgtgttttt atatcaatta
     1081 gttatgaaga gcaattgaaa ctgtctctgt tccaaagtaa cctgtttgtg cttgcaatac
     1141 attattttgt gcatttgctt gggcttcaga gatatcaaaa actattttac cagtttgttg
     1201 catatcagga tttaagcttt ccataaagaa agagttagtg atattaccat tttcatcttg
     1261 atttgccgac atagatgcag ccgaatcagc ttcgaaagtt tttccatctg cttttaattt
     1321 gaagaatgag ctatcaacag tcacagcctt atcacctgca ttttttacag ataagtcaac
     1381 tactaagaaa gtgtcttttg cgttagtagg aagtacagat gggccaactt gttttactac
     1441 ttcaacggag tttactttgt attccatttt ccctacagca acgtcatcac cgattttata
     1501 agtcttttct tcttttgctg tagtttcttt tgtagtttct ttattagtac ttgttgatgt
     1561 tgcagtagct gaatctttgt tttctccgcc acttagagca ccaccaatac caaaaaacaa
     1621 aagtaatatt aatacccaaa accacacttt tttgtaaaaa ggtttactaa ctttatacat
     1681 tttcccatct tgacccataa ctttttttgc catttaaata ttcctcattt cttgttataa
     1741 tatatttgtg atctcagaaa tgaggtatga gtccgtgttg cagcacgggc tttttttact
     1801 gtgcataaga gtattttttc ttgaaataag actggcaaac attaaaacat tctgttctta
     1861 acttattatt gatagaatag aactccatga aattttctaa tttgaactga gattcatcag
     1921 tcagttcgtt ctcaataaag atatttagta aaatcataat tgcgattcta tcagcttcag
     1981 tttcgaattt tgaatgaaaa gttgtagagt tatcgtacag tactgaatat tcaaaatgtg
     2041 aagcaatgaa atgaccgagc tcgtgggcta aatgaaaagc ttcagaactg tcttcgtgta
     2101 gtttttcatt cagaaatact attcttggtt ttggataata aaaacctggt tcttccattt
     2161 ccatatagat taactttaaa ttatactcac tcagcatttc tttcaacttt aaatacatac
     2221 aacccatcac tccaactatt cattttcctc taaagcttta gcaattgcaa tcgctttacg
     2281 cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt cttctgataa
     2341 atccacatgt ttaggggttt cttttttatc tcttcctaga agatagtcta tagaaacacc
     2401 aaaataatct gatacttttg ccaaaccttc ggagttaggt gatactgatt tccatttact
     2461 gaaatatcca tttgaatatc ccaatctttt ttctaattcc cttacggata ttccgttttg
     2521 ctttgttaat tcttttattt tttcgtatgg attcattgat atgtcaacct ttctagactg
//
LOCUS       DAHEYX010000234         2576 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHEYX010000234 DAHEYX010000000
VERSION     DAHEYX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.238"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            87..713
                     /locus_tag="MB219_002484"
     CDS             87..713
                     /locus_tag="MB219_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCC8428176.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            736..1440
                     /locus_tag="MB219_002485"
     CDS             736..1440
                     /locus_tag="MB219_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCC8428177.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            1632..2465
                     /locus_tag="MB219_002486"
     CDS             1632..2465
                     /locus_tag="MB219_002486"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCC8428178.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT          771 a          453 c          525 g          827 t
ORIGIN      
        1 gaaataagta cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa
       61 gttttggcta tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg
      121 aagaaaactg ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag
      181 atgcggaacg aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc
      241 ttacccatac tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa
      301 tcccagttta tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt
      361 ctggactcgg tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat
      421 ttgaaatgaa aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc
      481 attctatagg aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt
      541 ttagaggaag tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata
      601 gtattcggac ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg
      661 atgcaacgac gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact
      721 taggaggaga ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg
      781 ttcaatacaa ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa
      841 gaaggcattc attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct
      901 agtgacagtg gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga
      961 aaagaaattc cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa
     1021 ggtggatatg acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat
     1081 gatgaaatgt ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta
     1141 gataccgctg ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt
     1201 gaagatatcg cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat
     1261 ggtttgacga tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg
     1321 cttttaaatg gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc
     1381 atcaatgata cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa
     1441 tggggatatt ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg
     1501 tcttcttttt tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa
     1561 aaaacagatt gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa
     1621 ggaaggaatt tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag
     1681 gaataacgga atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca
     1741 tcaaactgga tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg
     1801 cgatcttggc tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat
     1861 caacggtaga aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac
     1921 cagccatcat cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct
     1981 taccagttgc cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca
     2041 acaaaaacaa aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc
     2101 ttgttgggtt attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga
     2161 tcctaggatc tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct
     2221 tagggattcc tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag
     2281 ggaattcatt caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg
     2341 tggtttccgt cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg
     2401 cttttggctg gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa
     2461 tgtagaaaaa accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt
     2521 aataataatc attataaata ataaaaacaa catcaagctt aatcaccaaa tgataa
//
LOCUS       DAHEYX010000235         2576 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHEYX010000235 DAHEYX010000000
VERSION     DAHEYX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.239"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            84..419
                     /locus_tag="MB219_002487"
     CDS             84..419
                     /locus_tag="MB219_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428179.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(495..2492)
                     /locus_tag="MB219_002488"
     CDS             complement(495..2492)
                     /locus_tag="MB219_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428180.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT          828 a          489 c          345 g          914 t
ORIGIN      
        1 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
       61 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
      121 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
      181 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
      241 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
      301 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
      361 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
      421 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
      481 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
      541 tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc
      601 aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta
      661 cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct
      721 tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca
      781 ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct
      841 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
      901 ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
      961 atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga
     1021 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     1081 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     1141 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     1201 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa
     1261 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
     1321 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
     1381 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
     1441 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
     1501 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
     1561 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
     1621 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
     1681 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
     1741 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
     1801 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
     1861 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
     1921 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
     1981 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
     2041 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
     2101 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
     2161 atagataaaa ccttttctat gcttaagtat ctactatcat ctttactcaa atctactacc
     2221 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
     2281 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
     2341 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
     2401 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca
     2461 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt
     2521 aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt tttagt
//
LOCUS       DAHEYX010000236         2571 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHEYX010000236 DAHEYX010000000
VERSION     DAHEYX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.240"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(1133..1798)
                     /locus_tag="MB219_002489"
     CDS             complement(1133..1798)
                     /locus_tag="MB219_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428181.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1924..>2571
                     /locus_tag="MB219_002490"
     CDS             1924..>2571
                     /locus_tag="MB219_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCC8428182.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFF"
BASE COUNT          828 a          407 c          462 g          874 t
ORIGIN      
        1 ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc tatatggaag ggtctgtggg
       61 gtaatactta aataaaggct aggaacctga taacatcaag gttcctagcc tttttataat
      121 aaaatgaagc gaaaataatt tcttttaaag agccgagatt atcagctata taagagtgaa
      181 tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg tagctctgct tatatgtttc
      241 gaatttaaga caaagagttg gcaaaagatg ttgataagga agataagata actgtgttat
      301 ataaactagg taactaactg taacaagcta attttaagag attcttatta gaagacgttt
      361 aggaatataa ctaagaaaat gattatacag gtaattgtca tttcaataat gttttctaat
      421 tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt cgtacgatag
      481 tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa cggccaggtt
      541 ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat taacaactga
      601 acaattttta caatgaaaaa attgttctgc cattactcaa agtctcttcc tccttgtatc
      661 gtttatttgt ttcattttat cattatgata ggcgcttgaa caatccattt tgtattttat
      721 tgagtactct tggataaact accagcgatt atcatattta tttatgttac tgtaagccat
      781 catataaaag taaatgagca gatcatagaa atataaaaaa gaactaaaat tgatctaaat
      841 tatgtgcagc ttgactacgt accaatctat attacttact agattggtcg ttaatctatt
      901 cggatcgaat gagcaaaata tttttgtgaa tgtccatcct tgattatgat tccaaaaaga
      961 atcattggga ggctgtggcg taaatattat actataagcc agaaatacct atcattctga
     1021 acttttttat ttaggaactt tcatctcaag gctttttcaa ttaatttgta cccctgtttt
     1081 ttggataaga tcatgtgcat tgattttttc tatctctacg ttcatctcct acttaatgcc
     1141 tatctggctc ctgagggctt ctttttaagg cgtcgtcata gtcttggctg aggatttctt
     1201 ggtaatctga gggaatggct tcccaagtga gcaggcgaac gttcatgacg tgttttcctt
     1261 tgtgtttgat tacagcatgg tttttctctt tttctacctc tccagcgtga aaagacagcc
     1321 atgtaccatg gccaaagata tcattacata cccaacgttc gatggaattg tccacgtatc
     1381 ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg
     1441 tttcattttt gtattgataa ttgataagaa aaagatagac caaacaaaag acgagcagaa
     1501 agaaagatag tgtctttttg agtgagaagg gttgttttga tgaataagat ttcttgttaa
     1561 caagaggttc atctggcaat gttggttcta ttgattcggt agctgattca atagaaggaa
     1621 aatcaatcgg cagacctaaa gtatgatgta aaagtgacag catttcaagg tctggcattg
     1681 tacgagaatt ttcccaacta gaaatcgttt tatcggaaac accaattttt tcgcctaatt
     1741 ctttttgcgt aagcttacaa tttctgcggt aagttcgtat tttttctccg gtgttcatct
     1801 tcttctcctt tcgattctac agtatataga tttattctac atgacctata cacttgatag
     1861 atacgttttt ttgatgaaaa acggtatgat tttatcaaag aatcatttaa gtaagggaga
     1921 gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac cggtatcatg
     1981 agtggttttg ccttggttag tataaataaa caggtactct atcggacaaa tgaatttttc
     2041 catcaaacag atacttatac tccgattcga ttgccacaac attttattgg gcaagaagaa
     2101 tatgagcagc taatcatggt atttgaaaat gtctcatcag aaactgagct ttcttatatg
     2161 aaaaaaacag taaatgaatc caatgaagta gatacatctt ggaacttcaa gatacagcca
     2221 aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc
     2281 tattctaata cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt
     2341 gaaggtgaat ttttcttgat gacgagagaa aaagaagcct atgaacggtt catttcacta
     2401 ttcactgatc gattcaatca agcatttcac actgattatg ccactaatat ttttgaagac
     2461 tttgaaaata gctattcgta ttcggttatt tcaaatacag acgtatataa ttatcgagtg
     2521 tatttaacta ttggattgat tcttttattc cttttgttat catccttttt c
//
LOCUS       DAHEYX010000237         2556 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHEYX010000237 DAHEYX010000000
VERSION     DAHEYX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.241"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            4..1455
                     /locus_tag="MB219_002491"
     CDS             4..1455
                     /locus_tag="MB219_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCC8428183.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1804..2475
                     /locus_tag="MB219_002492"
     CDS             1804..2475
                     /locus_tag="MB219_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCC8428184.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
BASE COUNT          953 a          366 c          504 g          733 t
ORIGIN      
        1 caaatgaaaa aatctttaaa acttgcatca gcagttatat taatgtccca actctttata
       61 gcaacacctt tccatgtgtt ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga
      121 accgagacga ctgaaatgag taattccgat gtaacagcag agagtagtga acttttgaca
      181 actttatcaa gtgaaagtag tacgacttca aatgctgagt cttcaacaac ttcatcaagt
      241 gaaagtagta cgacttcaaa tgccgagtct tcaacaactt cgtcaagtga aagtagtacg
      301 acttcaaatg ccgagtcttc aacaacttcg tcaagtgaaa gtagtacgat ttcaagtgcc
      361 gaatcttcaa caacttcaag tagtacaagt gaaataactt cttcgaaagt gacaagacca
      421 aaccatatag agtcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac aaaaaatttt
      481 tcgacaccac agcaaataga atcaacagaa tctttccaat cacaaccaat actatctggt
      541 aatgaagaaa ttcattttga caaaaatgaa tctgtagaaa attttattat gaaaattggt
      601 gagtctgcga gaaaaattgg tcagaaatat gatttatatg cttctgttat gattgcacaa
      661 gcgatcttag aatctgccag tggtcaaagc cagttagctc aggcaccgaa ttacaattta
      721 tttgggataa aaggaactta taatggaaac tttgtcatta tggtaacaaa cgaagatttg
      781 ggtaatggaa cgctttatac gacccaatca aaatttagag tgtatgaaaa ctatgaggaa
      841 agttttgaag attatgccaa attattaact aagggaatta gtggaaataa agatttttat
      901 gcaggagcat taaaagctaa tagtaaaaca tatcgagaag caactaaatt ccttacagga
      961 cggtatgcaa cagatacaca atactatttg aagctaaatg aattaattaa aacctatgat
     1021 ttaaccaact atgataaaga agttaaaggt ccacaaatga gtaagactgg ataccaagtg
     1081 cctattcata attattcaat aaccagtttg tttggaaatc gtggaaatga atttcaccga
     1141 ggacttgata tggcagctac acagggtgag ccgattcatg cagttaaagc tggtaaggtt
     1201 gttaaagcag agtttcacta ctcttgggga aattacgtgg tgatacaaca cgaagatgga
     1261 tcaaccgcgt tatatgctca ccagcaacaa tacctagtaa aaactggaga taatgttttg
     1321 caaggacaaa taattggcta tgtaggaagt actgggaata gtactggccc acaccttcat
     1381 ttggaaattt gcaaagatag tagtctttct cagagcatgt tagttgatcc gaaaacctta
     1441 ttattcggag actaaaaaag gtaatcaatc tgacaaagta acaagaaaat ggttggattc
     1501 ttagaccttt gatcaggata ttgagactgg aattgatatc tcgattacga tgagtttgac
     1561 aaataaaaca agtccattat cgaatatcga gtgtattatt gccgtcttta tgctcatatt
     1621 ttgagcaggt tttactagat gaaaacaatt tatctatctt gaggatttcg tgtacgtatc
     1681 gttcagtctt gtattataaa ttagtcataa aatcagacta agaaaaattg aaaatatttt
     1741 tttctgattt attaaaagtt aattgaaatt atatggtact tctttcttgg gagggtaggt
     1801 gcagtgaagg aaagatatgg tcaccttact gttgttgagg agattactca taatggatat
     1861 aaaaaatatc gttacctatg tgactgtggg aatgaaacga ttgtatatcg atcaaactta
     1921 ctatcaggta gaaccaaaag ttgtggatgt ggaaattata aaaataggca taaatataaa
     1981 aatttgacaa ataaaacttt tggtaagtta gttgcaatca agccaactaa actgcgaagt
     2041 gaaggaactg ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca
     2101 aagcgacttc taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac
     2161 attgaaaata aaaaattcgg attacttact gcaaagtatc ctttggacaa taactgttca
     2221 tggaaaacaa aatgggtttg tcaatgtgag tgtggcagta tgtgtgagat aagttatagt
     2281 aacttatttt ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca
     2341 tgtgttgaag ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa taatacaagt
     2401 ggtgttaagg gagtttatcg ttctaaagag aaatggattg cgtatataac tttaaaaaaa
     2461 agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagtt agaaaagatg
     2521 ctgaaaataa attgtttcaa cctttgattg acaaat
//
LOCUS       DAHEYX010000238         2515 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHEYX010000238 DAHEYX010000000
VERSION     DAHEYX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.242"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..2232)
                     /locus_tag="MB219_002493"
     CDS             complement(<1..2232)
                     /locus_tag="MB219_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428185.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT          686 a          586 c          434 g          809 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagtt
//
LOCUS       DAHEYX010000239         2488 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHEYX010000239 DAHEYX010000000
VERSION     DAHEYX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.243"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            2..1147
                     /locus_tag="MB219_002494"
     CDS             2..1147
                     /locus_tag="MB219_002494"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCC8428186.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="MB219_002495"
     CDS             1318..1776
                     /locus_tag="MB219_002495"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCC8428187.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="MB219_002496"
     CDS             1935..2405
                     /locus_tag="MB219_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCC8428188.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          826 a          470 c          469 g          723 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaataaa aaaagatgaa ctatttctag ttcatctttt tgcctttgcg
     2461 tggcgacgtc ctactctcac aaggggca
//
LOCUS       DAHEYX010000240         2454 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHEYX010000240 DAHEYX010000000
VERSION     DAHEYX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.244"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            42..980
                     /locus_tag="MB219_002497"
     CDS             42..980
                     /locus_tag="MB219_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCC8428189.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            1003..1683
                     /locus_tag="MB219_002498"
     CDS             1003..1683
                     /locus_tag="MB219_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCC8428190.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            1656..>2454
                     /locus_tag="MB219_002499"
     CDS             1656..>2454
                     /locus_tag="MB219_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCC8428191.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRIL"
BASE COUNT          760 a          428 c          557 g          709 t
ORIGIN      
        1 aatcgtaaaa ttccgatttg ttcattgaaa ggagcatata aatgaaaaaa tatcttttga
       61 cagctacggt aatgataggt gctttactgt ttgcagcatg tggaaatacg aacaaggaag
      121 cagacaaaaa agaggatttg acgatcgtta cgacttttta tccgatgtat gattttacaa
      181 aagagatcgt aggggatgaa ggaaacgtga aacttttgat ccctgctgga acggagccgc
      241 atgatttcga accaagtgca aaagaaagag cagaaatttc cgatgcagat gttttcgtgt
      301 ataatagttc agacatggaa ttttttgtgg attcgttaaa ggattcagtg gatagcaaac
      361 agaccttgat gatcgaagca gctaaaggaa tcgaccgttt agaatcgcaa gaagcagacg
      421 aacatgaaga atcagaagaa gggcatggtc attcacatga atacgatcca catgtttggc
      481 tcgatccggt attagctata aaagaagtca gaacgattgc tgaagaatta ggcgaaaaat
      541 atccagataa aaaggaaact tttacgaaga atgccgatgc ttatatcaaa aagcttgaag
      601 cactggatca aaaatactcc gatgagctaa aagatgcaac aaaccgcaca tttgtcactc
      661 agcatgctgc ttttgcttat ttagcgaatc aatacgactt aaaacaagta gcgatttcgg
      721 gcgtttcacc agatcaggaa cccacaccaa gtcgcttagc tgagctaaaa gaatttgtga
      781 agaaaaacaa catcaaagtg atttattttg aagaaaatgc ctcttcgaag gtagctgaga
      841 cattgtcgaa tgaaacagga gtgaagctag aagtgttgaa tcctttagaa agcttgacta
      901 atgaacaaat caaagcaggc gaaaattata tttctgtcat ggaaaaaaat ctgaaagctt
      961 taaaagaaag tatcaattaa gttttagaat ggaggttgta aggtgcgtta tatcgaaata
     1021 tctgatttga ccttttacta tgatgatgaa cctgtactag aagatgtctc ttatcatgta
     1081 gatgcgggtg aatttgtcat tttaactgga gaaaatggag cagccaagtc aaccttggtg
     1141 aaagcatcgt taggattact gaaaccttca aaaggaatga taaagattgc tgagaaaaat
     1201 caagaaggaa aaaaattaag tatcggttat attcctcaac aagttgcatc tttcaatgca
     1261 ggttttccta gtacggtgat cgaattagtc cgttcaggaa gatatccaag aggccgctgg
     1321 ttcaagcgtc tctctgaaaa agatcatcaa catgtacagc gtgctttaga agctgtaggg
     1381 atgtgggata tgcgaaatcg gcgaataggt gaattgtctg gcggacaaaa gcagcgaatc
     1441 agtttagcgc gtatttttgc tactgaccct gacttatttg ttcttgatga accaacaaca
     1501 ggtatggatg aagaatccag aaatgaattt tatcgtcttc tccgacatag tgcgcatgtc
     1561 catgggaaat cagttctaat gatcacccat gatcatgaag atatcaaaca gtatgcggac
     1621 agacagatcc gactggtgag aaaggaggat tctcaatggc gttgctttca tatgaattca
     1681 tgagaagagc gtttcttgcc gctatcttca ttgccggaat cgccccgatg ctaggtgtgt
     1741 ttttagtgat cagaagacaa tcgctgatgg ctgatacgct ttctcatgtc tctttagcag
     1801 gggtagcgtt aggattcttt ttgaacctta atcctacact tacgaccatg ctagtcgttg
     1861 tattagcagc tgtgatattg gagtatctgc gaacgatcta ccgaacctat tcagaaatct
     1921 ccattgccat tttaatggct ggtggtttgg cgctagcgtt agtattgatg aatttgagcg
     1981 gagggaattc agcaacaagt atccaatcct atctgtttgg ttcgatcgtg accattacat
     2041 gggagcaagt catcttttta ggaatcttat tcgttgccat cggtatattg ttctttttat
     2101 tcaaacggcc gatgtatgtt ctgacttttg atgaagatac cgcccacgtg gatgggctac
     2161 ccgttcattt gatgtcgatg atgttcaatg tgattactgg tgtggctatt gctgtgatga
     2221 tcccgattgc cggtgcactt ttgatatccg caatcatggt tcttcccgca gcaatcggta
     2281 tgcgattagg caaaagcttc aatgtcgtga ttgctatcag tgtttgtgta ggatttctag
     2341 ggatgctgtc aggactaact agctcgtttt atctagatac acctccagga gcaacaatca
     2401 cattagtctt tattgctttg ttcctgattg taaatatcat caaacgaata ttgg
//
LOCUS       DAHEYX010000241         2440 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHEYX010000241 DAHEYX010000000
VERSION     DAHEYX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.245"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(185..751)
                     /locus_tag="MB219_002500"
     CDS             complement(185..751)
                     /locus_tag="MB219_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCC8428192.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(751..1707)
                     /locus_tag="MB219_002501"
     CDS             complement(751..1707)
                     /locus_tag="MB219_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCC8428193.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1707..2363)
                     /locus_tag="MB219_002502"
     CDS             complement(1707..2363)
                     /locus_tag="MB219_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCC8428194.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          732 a          517 c          455 g          736 t
ORIGIN      
        1 cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgatttggtt
       61 ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa
      121 cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt
      181 ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt tggttgataa
      241 accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca
      301 tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc aatcgtccgc
      361 gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct ggcatagtga
      421 tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct ttgagatttt
      481 gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat gagacacgtt
      541 cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt tgctggatat
      601 caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc
      661 cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct tgcagtaatg
      721 tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc gtacatcata
      781 ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca agacttccca
      841 ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg
      901 atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat tgacatagtc
      961 aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg
     1021 catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa tggtgcgtct
     1081 gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca aatgcgtaca
     1141 aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt gataatcatg
     1201 tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa gttctaccca
     1261 taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg gcagacaatc
     1321 cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt gctcaaatcg
     1381 ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta
     1441 ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga attggattgg
     1501 aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc aaaaagtaca
     1561 accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat cgatcggcac
     1621 cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat gatatctttt
     1681 atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga gttatctatt
     1741 tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag actgagtaag
     1801 tagccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct tatcccgaca
     1861 cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct tttttgaaca
     1921 aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc tgcatgtcca
     1981 ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg ctctcgcatg
     2041 aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc agaaacgaac
     2101 caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca agcaaataaa
     2161 atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct gttcagatca
     2221 ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag ccacgacaga
     2281 tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct accagccacc
     2341 tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt attatagcat
     2401 ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
//
LOCUS       DAHEYX010000242         2431 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHEYX010000242 DAHEYX010000000
VERSION     DAHEYX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.246"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            355..1794
                     /locus_tag="MB219_002503"
                     /pseudo
     CDS             355..1794
                     /locus_tag="MB219_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2139..2306
                     /locus_tag="MB219_002504"
     CDS             2139..2306
                     /locus_tag="MB219_002504"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428195.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
BASE COUNT          685 a          434 c          545 g          767 t
ORIGIN      
        1 tagcaatgta tttgaaaagc aaattggaaa acaaataggt gcaacaccat aaatatgaaa
       61 aaattgggaa aaagtagaat ggaaattttc ttacgtctca cgagatagct cacactatta
      121 atctgaagaa caatgaagtc gacttttaga aattgctaga agaaagtaaa gcgaattatc
      181 actagttact atagagtaag ttcaaataaa gcatctcaaa cttacatggt tacatgagaa
      241 taacaaatcg aaattactca agcttcctat ggctagagtc gcgagaagtt gttgggcagt
      301 agacataggt tatattgttc ttagctacac gatcattaga aaggaaatcc tattatgcat
      361 agttatcaag aagatgcaaa agtccaaaaa aatcgctggt ggattttgat tgctatatct
      421 atgtttacct tcatgtccac attggatggc agtattgtca atattgcact acctaccatc
      481 tcagatgata tgggcatacc aatgaatcaa gcggaatggg tagtgtccgt ttatctgatg
      541 attgtctgtt cttgtttgtt gctgtttggt aaaattggcg atagctgtgg gaaaatcaaa
      601 gtatttcgga ttggtacgct gatctttgtc ttgggctcgc tgttgtgtgg gttcaatcat
      661 tcgctaggta tttaattgtt cgcacgggtg gtacaagcca ttggtgctag tatgactatg
      721 gctacaaatg ccggaatcat cacggaagtc tttcccatac atgaacgtgg tcgggcattg
      781 ggagcaatcg gtgcgcttgt ttcattaggc tcgattgctg gaccaggtct tggcggcttg
      841 attttgtccc aattttcttg ggcgtacatt ttttggatca atgtaccggt gggaatcgta
      901 acgatgttga ttgctgaaaa attcttacca aaagatatca ccatgagcgg ccatacaatc
      961 gatggagggg gctttgctgc cattgcggct acgattatca gcctttttgg aacaatcttt
     1021 ttggggcaag aaagtggctt tttccatcca gtcccccttg tactgttcgt aatttcagtg
     1081 attgcctttg tcagcttttt aaaagttgaa aagaaagcag cctctccatt gatttctttc
     1141 ggtattttta agaacaagat ttttaccatg agcttgatta cagccttgtt gattttttct
     1201 tccaacttct ttgttaatgt agtgattcct ttttatctgc aaaatgctcg cgggttgagt
     1261 cctagctatg ccggactgtt gatgatggtc tttcccttga tgatggtcgt cggggcgcct
     1321 ttaagcggat acctgaccga ccgggttggc ccggagattt ttgtattagt aggcttgggt
     1381 ttattgtcgg taacccaagt catgtatatg tttttgtcag tggactctcc tttgtggctg
     1441 tacattgtcg ctactgccat catgggtgcc ggcaatgcct tgttccagtc cccaaataat
     1501 acgatggtta tgagttctgt cactatcgaa aatttgggac tggcaggctc catgaattcc
     1561 tttgcccgaa atttggggat ggtcatcggg atttcgcttt ctacgacggt cttatatcaa
     1621 gcgatgtctg ctaagatggg ccaacgagta actaactata tcgcagatcg gccggatgtc
     1681 tttttatatg gaatgaagat taccttttta ggcagctttg ttttgtgttt tgttgccttt
     1741 ttcctgacta tctggcgtat caaaaaacaa ggaggtattc agaaaaatgt ctgaaaaacc
     1801 attgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     1861 agatgaagtg aacttctgaa aaccgaaaaa ctcaagtatt atcttcaaaa tactagtact
     1921 caattagatg ctcgatttga ttacaagaag actgcgaaag ccactggcaa ccgcaagcta
     1981 ggtatccacc gatggactac gttttagagg caactggtta tgaatacgga gctaacgctc
     2041 tcatctgcat aagaatgcaa cacccagatt atgtaaagat tttcgatact tctgtcaaaa
     2101 aaatcatcaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtggacaca
     2161 cagatggtaa gaagtcattc acaattcgag aatggacttg tcctatttgc cgtacacatc
     2221 atgatcgaaa cgtcaacgtt agtatcaata ttttgatcaa gggctatgta tacaagcatt
     2281 gccttagaaa acatcaagaa ccttaggaac aacggggata gattggttaa taagagatat
     2341 cgctgttagt aaagacatag gcttgcaagt atactctttt atcaagaatt ccgtggcttt
     2401 agtcatgata ggtttaatac tatgaaaatt t
//
LOCUS       DAHEYX010000243         2420 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHEYX010000243 DAHEYX010000000
VERSION     DAHEYX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.247"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            120..2273
                     /locus_tag="MB219_002505"
     CDS             120..2273
                     /locus_tag="MB219_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCC8428196.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVLVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          826 a          424 c          536 g          634 t
ORIGIN      
        1 aaagttgatt ttatcttgaa attaggtaaa gaatttcttg ctccgcccat tttttctttt
       61 ttattaagaa gtttttgggt acaatgaagc atgaatgtaa ataggcggaa ggataggaaa
      121 tgatgaaaga ttttatgaaa aaaatattaa atacttcttg gttagaaaaa ataagaaaag
      181 gtgcacaaga acctaagaac aaaacgtttc gccggtccca tgttcctttt cgcttaaatt
      241 ttcttttttt cattatattt acgttatttg ttgcgttgat tgctagattg ggttatttgc
      301 agatcgtgaa cggcgaagag atggaggcgc gagtgaagtc tacttctacg attacggtcc
      361 aagaaagtac tcctcgcgga atgatctacg actccacagg aaaagcttta gttacgaata
      421 aagcgaatca ggccatcaca tatacgagag gaacacaagt tacggctaaa gatttgcttg
      481 aaatcgcaac gaagctgaac tcgctgatcg atgttctggt agatgaaaat ctgacagata
      541 gagataaaaa agatttttgg ctggctgatc ccaaacattt gaaagaagcc acaaaaagac
      601 tttcggcaaa agaaaagcaa ttagggaaca gcgaacaata tagtgcgatg gtcaataaag
      661 tcacagctga tgagattaat ttaaacgagg agcaattaaa aattgctacc atctttaaac
      721 ggatgaatag tgcatatgaa ttaaacactg tctatatcaa aaactctggt gttactgatc
      781 aagagttagc tgttgtggca gaacgcgctt ctgaacttcc aggagtttcg actggcacag
      841 actggacacg agaatataat gcagcaagtt cactgaaaag tattcttggc agtgtgacga
      901 ctgaaaagca aggacttcca gcagatgaag cagagaaata cctagcaaaa ggttattctc
      961 ggaatgaccg tgtaggacaa agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa
     1021 ccaaaaccca atatgaagtt tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa
     1081 tcttctctgg ggaaaaaggt tctaatttaa tgttatctat gaatgcagaa ttccaatcaa
     1141 aagtagaaga aattttgaaa aggaattatc aaacattgat caacaacggc aaagcacagt
     1201 attcgcctgg tgcttatgca gtggctatga atcctcagac aggagaagtg ctagcaatga
     1261 ctggtttcag tcatgaacaa ggttccaaag aaatcaccga gaatgcttta ggcacgatta
     1321 ctagcgcctt tgatccaggg tctgtagtca aggctggtac tttgacagca ggctgggcaa
     1381 gtaacgcgat cagcggaaat caagttttga ttgatgagcc gattcgtctg caaggtgctt
     1441 cagaaaaaag ctctgtcttt aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat
     1501 cggaattgtc ttctaatacg tatatgatca aagtggcgtt aaagatgtta ggactagact
     1561 atacacctgg aatgggacta cctagtttag atgaagaagc taaagcttat cagcaattga
     1621 gagactcttt caaagagttt ggtcttggta caacgactgg gatcgatttg ccgaatgaat
     1681 ctcctgggat ctcacgttct gttgattata tgaagaagtt caatgcggat aatggcaaag
     1741 aatggtatgc tccaggtaac tttaccgatt tggcatttgg acagtttgac acatatacac
     1801 caatccaatt agctcaatat gcttcaacag tcgctaatgg cgggaaacga gtacagccgc
     1861 acctagtcaa agcgatctat ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag
     1921 aaattggcac aacagtagaa aatacggtca atatttctgc agaaaatatg gcgatcctcc
     1981 gtgaaggatt ccaccaagtt gtccatggta ccgatcctta cacaacagcc aaaccattag
     2041 ccagtgcgaa aatggacctt tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc
     2101 atccagatat tgcgacagtc aatagtaata tcgtggcata cggaccaaca gacaatccgg
     2161 aaattgccat cagtgttgtc ttgccgaact tattagacga acaagaccac ataaacttga
     2221 cgattgctaa agaaataatg gacgcgtatt atgatatgtt catggcaaaa taaaaaagag
     2281 gtcgtgaaaa agccgcttag tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca
     2341 catttttgct taaatttgac ttcataccga gaaactggct tttgaacacc gtttactcgg
     2401 caaaaaaggt tgtgacaaaa
//
LOCUS       DAHEYX010000244         2398 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHEYX010000244 DAHEYX010000000
VERSION     DAHEYX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.248"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1192
                     /locus_tag="MB219_002506"
     CDS             <1..1192
                     /locus_tag="MB219_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCC8428197.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1326..>2398
                     /locus_tag="MB219_002507"
     CDS             1326..>2398
                     /locus_tag="MB219_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCC8428198.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYI"
BASE COUNT          943 a          322 c          472 g          661 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggttatcga
      421 agtaacgtat ccagatggta cgaaagatac agtgaaagtt ccagtagaag tgacagacaa
      481 tcgttccgat gccgataaat acaatcctga aggacaaaaa gtgactacag acttaaataa
      541 agagccagac gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta
      601 tacttggaaa gagaaagtag atgttagtac agctggaaac aaaaaaggga ttgtagtagt
      661 aacatatcca gatggatcta aagaagaagt agaggttact atttctgtag aagataaaaa
      721 agcaccgaat aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa
      781 aactgaacca aatgcagagg tgacagttac attaccaaac gggagtcaat accatgggac
      841 agctgataaa aatggtaatt ttacagttaa agttcctaaa ttagaggcag gaacaaaagt
      901 gatagtaact gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc
      961 ttcaaacgaa aaagacagtg aaaaagccgt tagtaaagat aataagactg acaatcaagg
     1021 tagcaagcaa aacaaaaata gaggcaaaag cagtcctcaa aagcagtcct caaaagctta
     1081 tcctaagacg ggagagattg atagtaatat ctttactatt agtggtggtt taatcctgtt
     1141 aggaacttta gggttattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1201 tttcttaaat cttctctgaa aagaggaaga atctttttct tcctcttttt gttttttatt
     1261 ttgggagata ggttgttagt taaaaatgaa gatacttttc atagaaggga aaagaatgtg
     1321 attaagtgaa aaaaatagta attattggat caaaccattc ggggctagct gcagctcgat
     1381 ttctaaactt atgtgattca aattatgaag ttattctgat agataaaaaa tcagaaatta
     1441 gttacttaga atgtgggaca tcgttgttca taaaaagaga agttagcaat ttagaagatc
     1501 ttttttatca aaatagaaga gaactaaatt ctaagataaa aaagttgttt ttggagacag
     1561 cagttgacaa aattgatatt caaaataaaa agattatttg tgtaaaaaaa gatggcacaa
     1621 gattttccca agaatatgac aaacttatac tagctacagg ttcaaagcaa attatgttag
     1681 atgtaccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgctaaag
     1741 agatttttaa agctttagat gataatacta ttaaaaaagt aggaattatt ggagcaggat
     1801 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
     1861 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
     1921 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
     1981 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
     2041 tcgtacaagc tataggattt ctatctaata gtccaattgg caaaaaggaa ctcttgagag
     2101 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
     2161 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
     2221 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattgac aatcaagatt
     2281 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
     2341 cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat tatataga
//
LOCUS       DAHEYX010000245         2326 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHEYX010000245 DAHEYX010000000
VERSION     DAHEYX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.249"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            135..1997
                     /locus_tag="MB219_002508"
     CDS             135..1997
                     /locus_tag="MB219_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8428199.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1997..>2326
                     /locus_tag="MB219_002509"
     CDS             1997..>2326
                     /locus_tag="MB219_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCC8428200.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          835 a          345 c          457 g          689 t
ORIGIN      
        1 gtagtcgtct ttataataga taaactctgt aatattagtt aaaaagctat aagttgcact
       61 tagttaagtg caaacatttc atattgtcgt tagtttagaa aacgcttata ttagcaatgt
      121 aaggagagat gataatgcag cagctaacaa aaagagaaag gcagcttttg atttacttgg
      181 tggaaatgtc tgaaccggta acaatcaaaa agttggctga aaaagaaaat gttagtgttc
      241 ggacgataaa atacgatcta gatgatataa gagagtggct gaatgagcgg aagcaagtcc
      301 tatatagcaa acgtagtcag ggaatttgga tgaatattag tagctctgat cgaatcaaat
      361 taaaaagcga attgatggat gttaatcgtt ttgaaatatt tgcagatcaa agtctccgaa
      421 tcaaacgttt actaattatg cttttagtaa caaaagaagc tattactgga atgaaaatgg
      481 ccaacgcatt agatgtcagc aaagatacga tcatgaatga tttagatgtg ctggaaaata
      541 attttcttaa ggaaggattg accttgaatc gtcaggcgcg aaagggtttt tggatcatag
      601 gagaagaacg tctagttcga ttaacgattg aagagattct tcaaaaagag ttcacggatt
      661 atgacattta taaattaatg tcattgttat tgaatggcgg tgagactgaa tactttgaaa
      721 tgtattctgc tactgctaca cctatccaag aggtatttaa tcaagtaatc attcgaatgc
      781 gacacttact ggaatttgaa aatctagaga aactgaatta cgcggaatta ctaaatattc
      841 tgattcgagt gacaattgct accgttcgct tacgcaaaga ggctacgatt ggtcgttatc
      901 aattggttgc tgaacaagaa ttagacaaaa acgatctttc ttatcgcgtc atgcagcagg
      961 tctttgatta ttatcagttg ccactgttcg aggatgaata tcattatatc tatagtgata
     1021 cattcgacac gattcaccag caagacgtca tgaaactagt tgagaatgtg atttataaag
     1081 taagtaaagc aatcaaccat tctttttatc aagatccaca attgttgacg aatctgtatg
     1141 ctcatttatc catgcgatta tcgagaaaga aaaaatttgt caatgaatat aatcctttta
     1201 aagaagatat caagcagaaa tacccggaat tatttgatgc gatcaagcaa gcagttcgac
     1261 aagaaataag tgaacaaaat attctgatca atgattcttt cgtggcatat attgccttac
     1321 actttttggt ttcctacgaa agagaagaag atctacgaag cgttagagcc gtctacattt
     1381 gttcaacagg gttgggtgtg actagtctga tcaagcaaaa gatcaatgaa gaattaaata
     1441 atatcactat cgtggcattt gcttcagtat tgaatgcgca agaaatgatt gctaccaaag
     1501 atcctgattt ggtgattagt atttttccaa ttaatggcat cgatcgtcct ttcatcaaag
     1561 tccatccatt acccactgaa caggacttga aaaaaattca acaaataata aaaaaaatac
     1621 ttgcttcttc caatcagcag gaaaggcata acttgaatct tagagaaacg tccatcaata
     1681 aagatacttt agaagacttt agcaaggaat tgattttgaa agcttatacg atttatgaac
     1741 gtttacttgt cattttggaa gcagaattga tccaagaata tcgagaagct tttttgcttc
     1801 atgtattctt aatggttcat cgtatcacat ttgatcaaca atatatggca gaaggtcgtc
     1861 aagtagacca gattatttta gacaaaggaa cactggtagg aaaaatcgaa gcagtttttg
     1921 cagaaaataa tttaggaatc aatcagtcag aaatcagtgc attattctca tatataaaag
     1981 aaggagcgtc aagttagtga aaatgtcaga cgaggcttta aaaattatcg aggaaagtca
     2041 aaatcgggaa aagttaaaac aagtgattga tctaattgaa gagcgattag tagaacatgc
     2101 tattgtacca accgagttgc agtggaccat tctaattaat cacttgaatg agatgctgaa
     2161 acgttctcaa agaaaggaaa cgattcccgt agtcgatcgg gatttgttca aagaagtatc
     2221 caaagaagcc ataactattt ctgatgaaat agttagaagt atcggtaatt taaccgatga
     2281 tgaaatatat gttttgtcga ttcattttga aacagcaaaa aataat
//
LOCUS       DAHEYX010000246         2316 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHEYX010000246 DAHEYX010000000
VERSION     DAHEYX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.250"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            230..1006
                     /locus_tag="MB219_002510"
     CDS             230..1006
                     /locus_tag="MB219_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCC8428201.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1021..1224
                     /locus_tag="MB219_002511"
     CDS             1021..1224
                     /locus_tag="MB219_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428202.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1240..1578
                     /locus_tag="MB219_002512"
     CDS             1240..1578
                     /locus_tag="MB219_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCC8428203.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1565..1735
                     /locus_tag="MB219_002513"
     CDS             1565..1735
                     /locus_tag="MB219_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428204.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(1833..>2316)
                     /locus_tag="MB219_002514"
     CDS             complement(1833..>2316)
                     /locus_tag="MB219_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428205.1"
                     /translation="LIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSISGI
                     PDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITIPPY
                     ESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLDEFD
                     "
BASE COUNT          894 a          346 c          427 g          649 t
ORIGIN      
        1 gagttaaacc tctatacttg ttcttgtaaa caaatttaac aactaaaaag acaacaaaaa
       61 acacttttga ttattaaacg ccaaccgcca aaaaagcttt taaaatcaat gtttatatgt
      121 cttatttaac tatgtacaaa gtatagaata aaactctatg ctttgtcaat tgaatttaga
      181 aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata tgaacacacc
      241 gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg atgaaccata
      301 ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag atgctttgtc
      361 gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag gtcaatcaag
      421 agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat caaaacttcc
      481 ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca
      541 tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag atacaatcat
      601 tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag aacaaaagat
      661 tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta cagattcagt
      721 aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga ataaattact
      781 tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat
      841 gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg gtggcactaa
      901 aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg aattactaaa
      961 aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag gagttttagt
     1021 atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga atatgtcgaa
     1081 agttcaaagt tcttaactta caccatgctc gctgaaagat tcggcattag caaaagctac
     1141 ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaatagaat catagattcg
     1201 attattacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct agaagcgaaa
     1261 atcccagttc cagaaggcta tgtaattatc tcccaagtgg attatgagga gttaaaaaaa
     1321 gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat taacagaaaa
     1381 tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat tatcgatatc
     1441 gaaaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac atatctattt
     1501 ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt aaggaggcaa
     1561 gcggatggga aaatttaata gagcgctagt attcagcgca ccgctaatca tctacgcttt
     1621 aggactttgg ggaagcagac aagcgttgat aggaacgatt gtttacatgg tttggatttt
     1681 tatggggctg gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg actgactaat
     1741 gaaaaaaagt ttaatgacta taaacgaaaa acaattgaaa gaaaaattta atgatctcat
     1801 caaagaattt gtaaaagaaa ctggagaatt tcctaatcaa attcatctag ttgcggaggg
     1861 gcatagccgg tatcaagctg tgaaatttga gatgaagaaa caaatctatt gaatttagca
     1921 gtacctctac ttgtaagaac atttattgta atgttttttt cgactaaaga ttcatcgtat
     1981 ctaaaaacca acgtagtaat ttttgactca tacggtggaa tggtaatagg taatgacaac
     2041 ggaggaaatt ttgatagatc agcacctttt tttaaagatt taccagaata cgcacgtata
     2101 ccatgtggaa gttcgtcata cattgatttt accgttacag aataattatc accaatcatt
     2161 gtaaaagcat ttaaaggatc aggaattcca gaaatcgaaa actcaataat tgtaacaggt
     2221 aaagaactgt tatttgttaa caatacagaa tctataatcc tgaatttatt ttgatggtaa
     2281 acatctggtt tggaatcact ttctctatct atcaac
//
LOCUS       DAHEYX010000247         2309 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHEYX010000247 DAHEYX010000000
VERSION     DAHEYX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.251"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(1004..1294)
                     /locus_tag="MB219_002515"
     CDS             complement(1004..1294)
                     /locus_tag="MB219_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428206.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1502..2194)
                     /locus_tag="MB219_002516"
     CDS             complement(1502..2194)
                     /locus_tag="MB219_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCC8428207.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          664 a          435 c          334 g          876 t
ORIGIN      
        1 agtaatacta caattcctaa cataatcgta gaatttaata atttccatag cataaagtgt
       61 ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt cttataatgt
      121 tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac tacgtcatcc
      181 gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat ccctattagt
      241 aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat ttttatactc
      301 cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa gataacttaa
      361 aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag aaatctacga
      421 tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
      481 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
      541 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
      601 gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt ttacgactat
      661 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
      721 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
      781 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
      841 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
      901 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc
      961 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     1021 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     1081 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     1141 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     1201 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     1261 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     1321 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     1381 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     1441 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     1501 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     1561 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     1621 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     1681 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     1741 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     1801 tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat
     1861 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     1921 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     1981 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     2041 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     2101 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     2161 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     2221 ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     2281 aattttaata taaatactat caaatactc
//
LOCUS       DAHEYX010000248         2274 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHEYX010000248 DAHEYX010000000
VERSION     DAHEYX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.252"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            89..2254
                     /gene="acm"
                     /locus_tag="MB219_002517"
     CDS             89..2254
                     /gene="acm"
                     /locus_tag="MB219_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCC8428208.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          893 a          348 c          447 g          586 t
ORIGIN      
        1 aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt
       61 tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat
      121 gttatttatt attactaata taacatcact gattcctgtt catgtctatg cggatgcagg
      181 cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg
      241 aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga
      301 ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga
      361 aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc
      421 gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt
      481 tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt
      541 gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc
      601 tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg
      661 gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga
      721 aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg
      781 tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag
      841 cgctacattt aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt
      901 tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat
      961 aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg
     1021 agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt
     1081 tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt
     1141 gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt
     1201 gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt
     1261 aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa
     1321 attctccata aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa
     1381 ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat
     1441 ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac
     1501 aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga
     1561 tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt
     1621 cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt
     1681 catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc
     1741 gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag
     1801 tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga
     1861 aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta
     1921 taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag gtagccatat
     1981 aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc
     2041 tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat
     2101 tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac
     2161 aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc
     2221 tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttg
//
LOCUS       DAHEYX010000249         2271 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHEYX010000249 DAHEYX010000000
VERSION     DAHEYX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.253"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            45..980
                     /locus_tag="MB219_002518"
     CDS             45..980
                     /locus_tag="MB219_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8428209.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(1065..2075)
                     /gene="gap"
                     /locus_tag="MB219_002519"
     CDS             complement(1065..2075)
                     /gene="gap"
                     /locus_tag="MB219_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCC8428210.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT          722 a          407 c          476 g          666 t
ORIGIN      
        1 taattcaagg ttatatgaaa aaagagaaat gagatggatg atttatgaaa ctttggatga
       61 aaatcctttt aggcgtcgct ttagctctga ttgttgcgtt agtgtttgca gggaattatt
      121 tttataatta tgctgttgtg ccttctgaga aagacttttt agacgaagat acacctggta
      181 caacacagac atccaaagaa acggatgcac ataaatggtt tagttcaaaa gaaaaccgaa
      241 gcgattggac catcacttca aaagacggtc tgaaattatc agcgatttat cttcctgctg
      301 aaacgaaaag tgaaaaaaca gctcttgtgg ctcatggcta tatgggaaac gctgaaacga
      361 tgaccaatta cgcaaagatg tttcatgata tgggttacaa tgtccttgtt cctgatgcaa
      421 gaggacatgg aaaaagcgaa ggggattaca ttggatttgg atggccagag cgtaaagact
      481 atgtacagtg gataaacaaa gtgctagaaa acaatggaaa atcgcaagaa atcgttctat
      541 acggtgtcag catgggagca gcaacagtta tgatgactag cggagaaaaa ctaccagata
      601 atgtgaaagc aatcattgaa gattgcggtt attcttccgt tcatgatgag cttgcttatc
      661 agctagacga tatgtttagt cttccagcat ttccgcttat gcaagtaacg agtctagtga
      721 caaaagtccg tgctggctac tttttcggtg aagcaaatgc agtggaacaa ttgaagaaaa
      781 accagcgccc aatgctcttt atacatggag acgaagatac atttgtacca tttgaaatgt
      841 tagataaggt ctatcgtgca acgaagggac caaaagaaaa gtatgttgta aaaggagccg
      901 agcatgcgga agcatacaag acagatcctg aaaaatatca gcaagtagtc caacaatttt
      961 taagccaata tgttcattga acgagtttaa aagtagggga tgaaaagaaa gaagcctttg
     1021 actggatcca gtcaaaggct tcttttatcg attgatctat caaattacaa gttagcgaat
     1081 ttttctagta agcgaatcat ttggcaagta aatccatact cattgtcata ccatgcaaca
     1141 gttttaacaa gttggaaatc accagcagtt gttacttctg tttgcgttgg gtcaaagatc
     1201 gaaccttgag ttgttccaat gacatcgcta gaaacgattt gacggtcatc atacccgaat
     1261 gaaggattgt caactgtgtg ttttttgatt gcttcgttta cttgatcagc agttactttt
     1321 gttttcaata ctgaaaccaa ttcagttaat gaaccatcaa cgactggaac acgttgtgcg
     1381 tgtccttgta attttccttg tagctcaggg ataactaagc cgattgcttt cgcagcacca
     1441 gtagaatgtg ggatagtatt gtcagcagct gaacgagcag cacgcaaatt accgcctttt
     1501 acaggtccat ctaagagcat ttgtgtggaa gtgtaggcat gaactgttgt cattgtacca
     1561 acttcgatcc caaattcatt atttaagaaa taagccatag gtgctaaaca gtttgttgta
     1621 catgaacctg ctgagatgat tttgtcgttt tcatctaatg tatcatcatt gacgttatag
     1681 acgatggttt tcatagcacc agctggggca gaaataacta ctcgtttgac accagcatct
     1741 aaatgcgctt ttgctttttc ttcagatgta taaaaaccag tacattctag tacgatatct
     1801 acaccgtttt cttttgccca agggattttg cttgcgtcgg gttctgcata aacacgagtt
     1861 gcttcgccat ccactacgat tgagtcttct gttgcagtaa ctgtgccagg ataagtacca
     1921 tgagtagagt caaattgcaa taattgtgct aacatagttg gacttgttaa gtcgttgatt
     1981 gctactactt caatatcatc tgagacttct ttgatgcggc ggaaagccag acggccaata
     2041 cgcccaaatc cgttaatacc tacttttact gtcatatcaa aagttcctcc tttattaggg
     2101 gtgctgtgaa acggcatcct caattaatac aattaataca attaatacac tgttattctg
     2161 ctacgcaaag aatattttgt caaaaaatta tacttggtag cagaaaaaag gttggaaacc
     2221 ttaccaaatg ataaaaaaag aagaaggtaa acaatctttt tgctgaaatg a
//
LOCUS       DAHEYX010000250         2209 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHEYX010000250 DAHEYX010000000
VERSION     DAHEYX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.254"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(56..129)
                     /locus_tag="MB219_002520"
     tRNA            complement(56..129)
                     /locus_tag="MB219_002520"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93..95),aa:Ile,seq:gat)
     gene            complement(142..212)
                     /locus_tag="MB219_002521"
     tRNA            complement(142..212)
                     /locus_tag="MB219_002521"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Gly,seq:tcc)
     gene            complement(217..289)
                     /locus_tag="MB219_002522"
     tRNA            complement(217..289)
                     /locus_tag="MB219_002522"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Phe,seq:gaa)
     gene            complement(293..365)
                     /locus_tag="MB219_002523"
     tRNA            complement(293..365)
                     /locus_tag="MB219_002523"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Asp,seq:gtc)
     gene            complement(384..457)
                     /locus_tag="MB219_002524"
     tRNA            complement(384..457)
                     /locus_tag="MB219_002524"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Met,seq:cat)
     gene            complement(470..559)
                     /locus_tag="MB219_002525"
     tRNA            complement(470..559)
                     /locus_tag="MB219_002525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(521..523),aa:Ser,seq:tga)
     gene            complement(582..653)
                     /locus_tag="MB219_002526"
     tRNA            complement(582..653)
                     /locus_tag="MB219_002526"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(618..620),aa:Glu,seq:ttc)
     gene            complement(675..762)
                     /locus_tag="MB219_002527"
     tRNA            complement(675..762)
                     /locus_tag="MB219_002527"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(726..728),aa:Ser,seq:gct)
     gene            complement(787..860)
                     /locus_tag="MB219_002528"
     tRNA            complement(787..860)
                     /locus_tag="MB219_002528"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(824..826),aa:Ile,seq:gat)
     gene            complement(874..944)
                     /locus_tag="MB219_002529"
     tRNA            complement(874..944)
                     /locus_tag="MB219_002529"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(910..912),aa:Gly,seq:tcc)
     gene            complement(946..1021)
                     /locus_tag="MB219_002530"
     tRNA            complement(946..1021)
                     /locus_tag="MB219_002530"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(986..988),aa:Asp,seq:gtc)
     gene            complement(1040..1113)
                     /locus_tag="MB219_002531"
     tRNA            complement(1040..1113)
                     /locus_tag="MB219_002531"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Met,seq:cat)
     gene            complement(1126..1215)
                     /locus_tag="MB219_002532"
     tRNA            complement(1126..1215)
                     /locus_tag="MB219_002532"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1177..1179),aa:Ser,seq:tga)
     gene            complement(1239..1312)
                     /locus_tag="MB219_002533"
     tRNA            complement(1239..1312)
                     /locus_tag="MB219_002533"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1276..1278),aa:Ile,seq:cat)
     gene            complement(1326..1399)
                     /locus_tag="MB219_002534"
     tRNA            complement(1326..1399)
                     /locus_tag="MB219_002534"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1363..1365),aa:Met,seq:cat)
     gene            complement(1427..1499)
                     /locus_tag="MB219_002535"
     tRNA            complement(1427..1499)
                     /locus_tag="MB219_002535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1464..1466),aa:Ala,seq:tgc)
     gene            complement(1519..1592)
                     /locus_tag="MB219_002536"
     tRNA            complement(1519..1592)
                     /locus_tag="MB219_002536"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Pro,seq:tgg)
     gene            complement(1602..1675)
                     /locus_tag="MB219_002537"
     tRNA            complement(1602..1675)
                     /locus_tag="MB219_002537"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1639..1641),aa:Arg,seq:acg)
     gene            complement(1686..1771)
                     /locus_tag="MB219_002538"
     tRNA            complement(1686..1771)
                     /locus_tag="MB219_002538"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1735..1737),aa:Leu,seq:taa)
     gene            complement(1788..1859)
                     /locus_tag="MB219_002539"
     tRNA            complement(1788..1859)
                     /locus_tag="MB219_002539"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1825..1827),aa:Gly,seq:gcc)
     gene            complement(1871..1943)
                     /locus_tag="MB219_002540"
     tRNA            complement(1871..1943)
                     /locus_tag="MB219_002540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:Thr,seq:tgt)
     gene            complement(1961..2042)
                     /locus_tag="MB219_002541"
     tRNA            complement(1961..2042)
                     /locus_tag="MB219_002541"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2006..2008),aa:Leu,seq:tag)
     gene            complement(2050..2122)
                     /locus_tag="MB219_002542"
     tRNA            complement(2050..2122)
                     /locus_tag="MB219_002542"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2087..2089),aa:Lys,seq:ttt)
     gene            complement(2126..2198)
                     /locus_tag="MB219_002543"
     tRNA            complement(2126..2198)
                     /locus_tag="MB219_002543"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2163..2165),aa:Val,seq:tac)
BASE COUNT          591 a          616 c          527 g          475 t
ORIGIN      
        1 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaataaa ataaatgggc
       61 ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc
      121 tataggccca taaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga
      181 aggctgtggt tttaccacta aactacaccc gcataatggc gcgggacaga atcgaactgc
      241 cgacacatgg agcttcaatc cattgctcta ccaactgagc taccgagcca aacggttccg
      301 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
      361 gaaccaatgt ttttagtttt taattgcggg agcaggattt gaacctacga ccttcgggtt
      421 atgagcccga cgagctacca gactgctcca tcccgcgata atatgaaaaa ggaggataag
      481 ggattcgaac ccttgcacgg ttttacccgc ctgacggttt tcaagaccgt tcccttcagc
      541 cggacttggg taatcctcca tgtaaaacta tattaaatta atgacccgta cgggattcga
      601 acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg gccaggtaaa
      661 acaaaatatt gaaacggaga aggagggatt tgaaccctcg cgccggtttc ccgacctaca
      721 cccttagcag gggcgcctct tcagccactt gagtacttcc ccaaaaccaa taagattttg
      781 tactaatggg cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta
      841 accagctgag ctataggccc ataaaaaact aaaagcgggt gacgagaatc gaactcgcga
      901 caacagcttg gaaggctgtg gttttaccac taaactacac ccgcatggcg gttccgacgg
      961 gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac accacggaac
     1021 cagtgttttt agtttttaat tgcgggagca ggatttgaac ctacgacctt cgggttatga
     1081 gcccgacgag ctaccagact gctccatccc gcgataatat gaaaaaggag gataagggat
     1141 tcgaaccctt gcacggtttt acccgcctga cggttttcaa gaccgttccc ttcagccgga
     1201 cttgggtaat cctccatatg aaacttacaa cgccacaatg gaccttgtag gactcgaacc
     1261 tacgaccgga cggttatgag ccgtctgctc taaccaactg agctaaaggt ccaggctctt
     1321 aaaaatcgcg gcggagggga tcgaaccccc gacctcccgg gtatgaaccg gacgctctag
     1381 ccagctgagc tacaccgcga aaaagatatt cattatttaa atgaaatgga gcctagcggg
     1441 atcgaaccgc tgacctcctg cgtgcaaagc aggcgctctc ccagctgagc taaggccccg
     1501 tgtttaaatt tataattatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa
     1561 ccaagtgctc taccaagctg agctacttcc cgtttaatta acgcgcccaa gaggagtcga
     1621 acccctaacc ttttgatccg tagtcaaaca ctctatccag ttgagctatg ggcgcatatt
     1681 tgaaatgccg aggaccggaa tcgaaccggt acggtgatca ctcaccgcag gattttaagt
     1741 cctgtgcgtc tgccagttcc gccaccccgg cgcgattgct ttggcaaagc ggaaaacggg
     1801 gttcgaaccc gcgaccccca ccttggcaag gtggtgctct accactgagc tatttccgcg
     1861 tgtttaaaga tgccggctaa aggacttgaa ccctcgaccc tctgattaca aatcagatgc
     1921 tctaccaact gagctaagcc ggcatgtaaa acttaaatta tgcgggtgaa gggacttgaa
     1981 cccccacgcc gtaaggcgct agatcctaaa tctagtgcgt ctgccaattc cgccacaccc
     2041 gcaaaaatat gagccgtaca gggctcgaac ctgtgaccct ctgattaaaa gtcagatgct
     2101 ctaccaactg agctaacggc tcatatggag gttaacggga tcgaaccgct gaccctctgc
     2161 ttgtaaggca gatgctctcc cagctgagct aaacctccat ggataactt
//
LOCUS       DAHEYX010000251         2202 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHEYX010000251 DAHEYX010000000
VERSION     DAHEYX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.255"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..956
                     /locus_tag="MB219_002544"
     CDS             <1..956
                     /locus_tag="MB219_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCC8428211.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
     gene            complement(1593..2002)
                     /gene="tnpA"
                     /locus_tag="MB219_002545"
                     /pseudo
     CDS             complement(1593..2002)
                     /gene="tnpA"
                     /locus_tag="MB219_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          712 a          396 c          343 g          751 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcattgcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc aatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 taacattttt tgaataccta aaagaacgcg gtttacaagg tacagaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgca agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gctcgtgagg
      661 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
      721 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
      781 gactaaagag taccaatcta attgaacgac tgaatcaaga agtgcgcaga agagaaaaaa
      841 ttattcgtat ctttccaaac tgcgtttcag ccaatcgttt aattggagca gtccttattg
      901 atcaacacga tgagtggctt tcttcatcaa gaaaatatat taaatttgcc aagtaaaaaa
      961 tagtgagagc attgtatagt attttacaca ggattgtgga cttgactttt ttttcattta
     1021 ttatctatta ttctctttta aaaatagccc caatgatctt atgtaagtaa acggacggac
     1081 aatttataat atagcacttt gcttcttgtt gcttgtatgg ttatttttgt gtttaaaatc
     1141 taaaagtcta actaaaattc tacctttaaa tttcagattc ttgaatgccc aatttatata
     1201 gagcactatc atgcttcttt tgttcttgga cgcaatttct tctggtctat tcattcaatc
     1261 ctagtgtatt tacacaatat tcctttggcc taaaactttg ataaatacgt atatataaat
     1321 ttacgtattt atcaaaaata taaatgcaat ttttactttc agataactct taaaacaacc
     1381 tactatcagg tagttttaag agttcactca ctttgcgaat ccaagcgggt cttgaagtaa
     1441 taatagtaaa atcccctaaa aaaattgtca ttctatagac ccttaaatgg acttatctcc
     1501 aaaagttaaa ctatttttcg gtattttact ggaggtagtc catttttacc cttttgttat
     1561 tgcaacatct actctctttt acttatacta tctcaaatgc gatcatcagc ttcttgttca
     1621 cggatgtatt tttcaattgt cttttgattc aattcgactg tactcacata atattcctcc
     1681 gaccaaaatg atttatttcc gtatctatat ttgtgattta cgtgctgctc gtggattagc
     1741 acagcactac gatctttcag ataactataa aactggatat acttattttt gatggtactt
     1801 gaacaagcat gtgaatatga tctggcgttg catgtgcttc tataatttcc acgtttttta
     1861 ttttgcacaa cttcctaagt atttttccaa tatcttttcg taattgccta tagattactt
     1921 ttcgtcgata ttttggtgta aatacaatat gatacttaaa attccaacga gtatgggcta
     1981 aacttttatc gtcatttgac atgaaaaaac tcctttcgat atgctagttc ttccacgtgt
     2041 agaaacgcat ggttttcttg tttcgcacgc tttgcgcacg aaactgactg aagtcattaa
     2101 caaaaaaatt gtagacaaag aaatttttct ttgtctacaa tctgaaagga taggcactac
     2161 cattttttat aaaatatttg tattgaacca ttttttagtt at
//
LOCUS       DAHEYX010000252         2200 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHEYX010000252 DAHEYX010000000
VERSION     DAHEYX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.256"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            731..1249
                     /locus_tag="MB219_002546"
     CDS             731..1249
                     /locus_tag="MB219_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCC8428212.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          765 a          311 c          415 g          709 t
ORIGIN      
        1 aaacaatcac aaacgtaggt tttaatgtca taatcgttag atattttagt tatttttcat
       61 tcaaaaaaac agggaactag tgtttgcata tgtatttttt tgagttataa taattcatga
      121 agagaggtaa ttcacttatt tacccaagct gaaaagcctg atattacttc ttttcaggca
      181 gtgctgctaa tattcaatta cacttttttg tataaattta gttgttttaa atttaattgt
      241 ctgctatgct tgtgatgaat tgattttcgt tctgcacttt tggacgataa atttggacaa
      301 ttgaaaatac aaaaaaaaag aaaggccctg tgagactcgg aaggctacca accttcgtcg
      361 acgtccacag gtggcattaa gatcactccc ttaatgcgtt tacctttcag tgttacggta
      421 ttacccgtaa cggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt
      481 gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcataa tgttataaaa
      541 atgtccgtgt ggctctttgc cgtatgacga acgctgaaat taggagacgt tgggatattt
      601 tataaatcat tcacatatga gttagacagt caatgcatat cagatatgca ttgtagaacg
      661 gattaaaata tcgtggatga aattatagat gaattaaagg agacaattag gaatttatgg
      721 aggtaatata atgagaacat ataaaaataa agaagaatta aaaaaagaaa taaataggac
      781 ttatactaaa tatattttag aatttgaaac tattcctgaa gaattgaagg ataaaagagt
      841 tgaggaagtg gatagaacac ctgctgaaaa tttagcatat caagttggtt ggacgaattt
      901 aattctaaaa tgggaaaatg atgaaagaaa tggattatct gttaaaacgc cttctgatca
      961 atttaaatgg aatcagttag gagagttata taagtggttt acaaatacct atgcacatct
     1021 accattgaaa gaattagagg aaattctcaa tcgtaatatt gatgatatta acatgatgat
     1081 tgattcaatg agtgatgaag atctattcac tgcacacaaa agaaaatggg cagacgaggc
     1141 aaccaaaacg gctgtatggg aagtatataa atttattcat gtaaatacag ttgctccatt
     1201 tgggactttt agaacaaaga taagaaaatg gaagagatta gctttgtaaa ttcccgttta
     1261 ctgctaagca attgtggaaa ggagacctct tgtatgttaa cagtaagaac atacgactac
     1321 ataagttctt tgctaactgc cggtttttat tgggtgaatg caagtgcact cccctagaat
     1381 gccgtagagg gtgcatcgtt agattgtatc aattttggtt ggccaacaga taaaccatga
     1441 ctcacttcga taaaatgaga ttatggtgcc atatccgcta caaagggact taggagacta
     1501 gcaggggaaa gattttttta tttttgggat gtttttcaga ccgccgatag taccgacaat
     1561 tccctttaaa tatggaatct caacaaccca catgaagggt aaggcaagtc cggtaattat
     1621 atcttctgac tttgggttag gaagaattga aattgttaag aacgattcaa cgcttatggt
     1681 ttataatttg gagatagcct ttgttaatat gctaaagcca acatacgata cagattttga
     1741 gcagtttatt ctaaccatca aagggtatgt ggcctatcgc ctatcctatt actgatactg
     1801 aagagatctt aaataaattc tatacgggat ttgaagatcc tgaaggtgaa atcgtcattt
     1861 atcagaataa ataaatcaaa tagtagaagt tcaaagaatg aggggaacat tagtttttat
     1921 tgcttagttt aatcccagta ccgatttctg caagatgtaa aaggtctttc atttcataag
     1981 ttttcagaag caagttcata cgcactaact cactttcgat tgagtagcaa tcgtcaacaa
     2041 actttactat cagctgttat ttttattctt tcgtgttatc taaataccat caatttataa
     2101 gttgtaggtg tatttaataa aagaacaaat aactttagta tttttattaa tctaacttac
     2161 aaactagtag tataggggta aatttgcgat tattcataaa
//
LOCUS       DAHEYX010000253         2155 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHEYX010000253 DAHEYX010000000
VERSION     DAHEYX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.257"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            28..492
                     /locus_tag="MB219_002547"
     CDS             28..492
                     /locus_tag="MB219_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCC8428213.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            506..1255
                     /locus_tag="MB219_002548"
     CDS             506..1255
                     /locus_tag="MB219_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8428214.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1255..2085
                     /locus_tag="MB219_002549"
     CDS             1255..2085
                     /locus_tag="MB219_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCC8428215.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          688 a          289 c          432 g          746 t
ORIGIN      
        1 attttaaaaa ttgaaggagg aaatagaatg attaaattag tgcgtgtaga ccacagatta
       61 cttcatggac aagtaatatt ttcttggact aaacagtatg atattaacca tattattgtg
      121 gctgataaca aagttccagg agatccaatg tctgtgatgg ctttgacaat tgctaaacct
      181 gctgattgca aattagacat tgttaaaatt agtgatgtta aagggattat ggaaacatcc
      241 ccaaatgata attacatgat tttaattaaa ggacctaagg aagcttttga attagttcaa
      301 aatttaccag aaattaaaga aattaatttt ggcggtgtgt ctaagaaaag cgagtccaaa
      361 caatatggaa aggcagtctt tttaactcct gaagaattag aagccacaaa aaaaattctt
      421 gatcaagaag tgaaaatatt tgtgcaacaa gtcccatctt caagtattga aaatgtagat
      481 tttagtaagt aaggggggaa aataaatgtt attaaaagcc attttattag gtttagttgg
      541 tgtggtagct gttattgact ccaggttaat tggtcgacaa aatattggta gaccactaat
      601 tttgagtaca cttgccggac ttgtgctagg tgacttgcat acagggattg ttcttggggc
      661 atctttagaa ttaatttcta tgggattcgt ctcaattggg gcggctggac ctcctaatat
      721 gcaacttggt agtattattg ccactgcttt tgcaatttta actaatagta gtacagaaac
      781 agccttaaca atagctatcc cagtggctgt agctggtgaa tttatgagta ttatcatgcg
      841 tatgataatt gctcaatttg gacatgctgc agataaagcc attgaaaata ataaatttag
      901 aaaagctcaa attattcata tttattggtc atttattttt aatgcttttg tttactttat
      961 tccaatcttt ttaacagtat attttggtgc tgacgttgtg gcagatttag tagataaaat
     1021 tccagaaatg attacagacg ccttgactgt ctctggaaat atgctttccg cattagggtt
     1081 tgcaatgcta ttaagcacaa tgttgagtaa aaaattatat ccttacttta tttttggatt
     1141 ctttattgtt gcctattcag gtctttcact aattggcgtg acaatttttg cagctctgat
     1201 tgcctttgta atggatcaag ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa
     1261 aatagtacag aaaaaaaacg tttaatcaca aaaaaagatc ttcataagat gtgtcttcgt
     1321 tcatggacaa tcatgtgttc gtggaattat gaaagacaaa tgcatatggg atttatgtat
     1381 gggatggcgc caattttaaa taaagtttat aaaaatgatg aagagaaaaa aagagaagct
     1441 tatactcgtc atatggcatt ttttaactgt acgcctcaaa tgactccatt tattatggga
     1501 ctttgtgctt ctatggaaga acaaaatgct caagcagaag aaggaaactt taatactgaa
     1561 tctatctcca tgattaaaac cagtttaatg ggtccttttg ctggaattgg tgattcattt
     1621 ttccaaggta ctttaagaat tattacattt gggattggat tgtcttttgc taagcaaggt
     1681 agtattttag gaccgatttt agctgttggc ctttttgcta taccttctat tttagctgtt
     1741 ttttacggcg gtaaattagg atatgagtct ggcaataaat accttactaa attgtatcaa
     1801 gaaggtctta tggatcgagt aatgcaattt gcttctatca ttggtttggc tgtagtaggt
     1861 ggaatggtgg ctagcatggt gccaattgtc acccctttaa aattcactac agggggaaca
     1921 gaacttgttt tacaagactt acttgattcc attattcctg gattattacc acttgtatgt
     1981 actttgggaa tatataaatt gattaagaaa aatgttagta caaatatgct cttattaggt
     2041 tgtattttgg cagggtttgt tttaagtata ataggtatct tataatgatt aaggctctaa
     2101 tttttgatat ggacggagtt ttaattgata gcgagattat gtatcaaaag tgggt
//
LOCUS       DAHEYX010000254         2141 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHEYX010000254 DAHEYX010000000
VERSION     DAHEYX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.258"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(342..1043)
                     /locus_tag="MB219_002550"
     CDS             complement(342..1043)
                     /locus_tag="MB219_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCC8428216.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1059..1499)
                     /locus_tag="MB219_002551"
     CDS             complement(1059..1499)
                     /locus_tag="MB219_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCC8428217.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1499..2074)
                     /locus_tag="MB219_002552"
     CDS             complement(1499..2074)
                     /locus_tag="MB219_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCC8428218.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          640 a          452 c          323 g          726 t
ORIGIN      
        1 atgaccaagg aagcttgagc atatgcgaca actgtcacaa actagtcaaa gattcattta
       61 aaacatttga atcttatcct gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc
      121 caagcaaaac tcactatggc tgatttttat gaacttcaaa ctcagcttct tcctttgatc
      181 aagcttcatc agtaaaacaa acagtggatc atttcagttt acaaatcaaa gagtaaagat
      241 ttatttattt caaatatttg aatataaaat aaccaaacga tagaaaggct tattctacat
      301 cacgtagtaa tttttctttc ccgtcgtttg gttttttatt tttagcctaa ttcttgacct
      361 tttgcatgct ggttatccgt aaaatatttt ccagcaggct ttccttccaa ttcagctaat
      421 ttttcttgga tctgacggat cacctttttt gcatcttctg gattttctac aaaatcgaat
      481 ttatccccat ccacttgtat tttaggacat acatcaaaat cttcatacca ttgattatag
      541 cgggtgttca acgtttcata ataggtatat aacgtctcat cgctttctag ctgttcatag
      601 tcacgcccac gttttttgat acgttctagc atcgtgtcca aagaaacttt gatatggacc
      661 atcaaatcag gccgtttttt tggagcaaca tcatctaact cgtttagcat cgtatctaat
      721 aagctatcgt actgctgtac ttcgatatcc gttacccgac ctaaatcagc gtttagatgg
      781 aacaacaggc tatcttcata gatggatcgg tctagcacat tgtcatcgtt cactaaagca
      841 tttttcattg ctaaaaatcg cttgttcaaa aagaagattt gcaataaaaa agcgtactgc
      901 tcaggatttg aataaaagag cggcaatact tcgttatctt cgacattttc ataaaatggt
      961 ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc tcttacctgc accaatcgtt
     1021 cctgaaatca aaatcactga catacaaccc ttcctcttct ataagtactc gcctacgtaa
     1081 gtttcaacag gcaatgtttc aaaatcatat tcacgtacag cttggtctga ttttaaatac
     1141 gtatggagac tttgtgtaat catcaagttc gtaggtaccg tctcttcttg tacaatgatc
     1201 atcggttttt tcaacatagt tgccacacct aattcgtaag ccgttcctgg atcgattgtt
     1261 tgatgctcaa agtcaaggat agcaacaaca aattctgcat ttgtcagttc ttccatatct
     1321 ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt cataattgct ttcttggtga
     1381 tgacgtggag agtagaaact tgtgactgtt ttgttttcct ccaacgcttt ttcgatacga
     1441 cttactcgat cgatttgctc ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt
     1501 attttgcctc cttcaataat aactgatttt taatagatac tgctcgtaat tcattgacca
     1561 gttccaatgg attgttggaa aagattactt gatccaacca ttttttatcc ataaaatcct
     1621 cttttgcaaa agtttctaat aattgttgca agccgctgta ataaccgcca atattcaata
     1681 ttccgattgg cttgtcgggc gtcagaccga ttgctatcca agaaagaagt tgtgagaatt
     1741 cctccaatgt acctagacct ccagggaaaa cgaggacagc atctcccttt tcaaataata
     1801 atacctttct ttcatcgatc gtttctgtag gaataaatgt agtaacatct tttccaggca
     1861 attcagtttc aaaaagccca ttcggataga tacctgtgac ttttccgtta ttttccagta
     1921 ctgttttact aatcactccc ataatcccgg agtatgaacc accgtaaacc agttcgattt
     1981 cttcttgtgc catgtatttt cctaacgttt gcgcaataaa tttgtaactt tctttattac
     2041 caaaacggga accgcaaaaa acagtaagtt tcataaaaaa acactcccta actcaaaagt
     2101 ctatcttcat tctacacgtg tttttttaaa ataaaagact a
//
LOCUS       DAHEYX010000255         2126 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHEYX010000255 DAHEYX010000000
VERSION     DAHEYX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.259"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..977
                     /locus_tag="MB219_002553"
     CDS             <1..977
                     /locus_tag="MB219_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCC8428219.1"
                     /translation="SLYWFVIQYGIKIPIKYKYYPVRLGWLGNRLFGVYTDPNFGAVI
                     SLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFSFIALSGSRTTLVCLLITTFVFSF
                     FTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVTDITAHAWAHVPHITEVKKDTSDS
                     SGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEIFKSKPISGGSPGYYIEYAHNKLP
                     YTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLFIQCFKALRYCFTQPNLVKDKHFF
                     AILMILTIAVSALFITELILVSTIGSFIFWGYLGSIQKTISKEEGVTN"
     gene            1111..2055
                     /locus_tag="MB219_002554"
     CDS             1111..2055
                     /locus_tag="MB219_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCC8428220.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          716 a          309 c          385 g          716 t
ORIGIN      
        1 tatcattata ctggtttgtc attcaatatg gcataaaaat tccaataaaa tataagtatt
       61 acccagttcg tttagggtgg ttaggcaacc gtttatttgg tgtatataca gatccaaatt
      121 ttggtgcggt tatatcatta atcgtcatca ttattgctat atattatttg gtcacttaca
      181 agcatttaaa tcttctattt aaaatatttt ttgtaattaa tatttttatt caattctcgt
      241 ttattgcttt atcgggttca cgcaccacat tagtttgttt attaataaca acttttgttt
      301 ttagtttctt tacaagttat agtagtcgtt ttcttaaaac taatacttta aaaagatggc
      361 taactgcatt agtagtagga ttgctaagta taattgcgat gtattatgta acagatataa
      421 ctgctcatgc atgggcacat gttccgcata ttacagaagt aaaaaaagat acatctgatt
      481 catcaggaac aactagcagc aataaagttg atttaaaacg ccctgacact gatagcgggg
      541 atgtttctaa tttaaggttt gaactttgga aaagtgcaat tgaaatattc aagagtaagc
      601 cgatatccgg aggttctcca ggctactata ttgaatatgc acataataaa ctaccatata
      661 cattaatggg aaaagatact ttaactgctc ataattttat tttcttagtg atggctgcta
      721 caggaggatt aggtctattg acattgttaa ccttcttatt tatccaatgc tttaaagcct
      781 tgcgttattg tttcacacaa ccaaatctag taaaagataa gcactttttt gctattttaa
      841 tgattcttac aatagcagtt tcggcattat ttatcacaga actcattttg gttagtacga
      901 taggttcatt tattttttgg ggatatctgg gttctatcca aaaaactata tctaaagaag
      961 aaggagtaac taattgaaac tatttttagg tatatgaaag tagaaaagaa aactagttat
     1021 cccaacttca acaaatttta tagtttttac aatataaaaa tggtaaacta atattatttg
     1081 atacgaaaat gtgcaattgg agtgaatttg atggacacta tattgattac tggcggagct
     1141 ggttttatcg gttcaacgct agcgaattat ttgggaaaag aaaacaaaat cgtagtagtt
     1201 gacgatttat caatgggaaa aaaagaaaac ttggatatgg ataaacaagt gactttcatt
     1261 gagggggatg tatcagattc tcaattgatg gaaagaatca tgaaagaata tcagtttgcc
     1321 tatatttttc atctagctgc tgtagctagt gtggcagatt ctgtagaacg gcctgtagaa
     1381 acacatcgtg taaattttga cagtgcgctt ttattgcttg agttagcacg gaaatatcaa
     1441 tctaatttaa aacgattagt atttagttca tcagctgcag tttatggtga tgaaccaaca
     1501 ctgccaaaaa aagaagaatc agtcatccgt ccattaacac catatgctat cgataaattt
     1561 gcagcagaac aatatgtttt ggattactgt catttatatg atgttccgac tagtgctgtc
     1621 cgatttttta acgtttatgg tccaaatcaa aatccaaact ctccatatag cggagtcatt
     1681 tcgattctag tggatcgtta caaaaaacaa ttggcaggag aaaaagcgga atttacttta
     1741 tttggcgatg ggagtcagtc tagagacttt gtttatatag aagatgttat acaagcactt
     1801 ttacttgttg ccaaggaaga aaaagcttta ggacagcaat tcaatgtcgg tacaggaaaa
     1861 agcactacgc tacttgaatt gatccattca attgatcaaa tcttaggaac agagctagcg
     1921 cttaaatatg aagcggagcg ttcaggggat attcgtgatt ccttagcgga tatttcaaaa
     1981 attcgttctt taggctatca gcctaaattt gatatattga gcggaatgga acggtattta
     2041 aagacagaaa tttgatgaaa aaatgattgc atctttggat aaataattct aaaaagtaaa
     2101 acaagaatta aagttgtata accaac
//
LOCUS       DAHEYX010000256         2123 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHEYX010000256 DAHEYX010000000
VERSION     DAHEYX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.260"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            13..498
                     /locus_tag="MB219_002555"
     CDS             13..498
                     /locus_tag="MB219_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428221.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            954..1367
                     /locus_tag="MB219_002556"
     CDS             954..1367
                     /locus_tag="MB219_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCC8428222.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          664 a          338 c          356 g          765 t
ORIGIN      
        1 ggaggagcta tcatgcataa agggatttac gcaggcttgt tgggaactgt aatacttttt
       61 tctgcctgtg gatataaaac aaagaattct gttaaagaat cttcagcagc catttcaaca
      121 actgaggttg gttcaactaa aagtacttct actacttcca ttactaatac ttctactggt
      181 aatgtagatt cgcaacatag ccaatcaagt attcagtcgt ttagcatttc ttcaacatct
      241 gaaatgcaag aaagcactgt accttctctt ccattagaaa actctgacaa agcacttcta
      301 tttcttattg cccaccgttc cgaattacaa agtgacgata ttgttattag cttttatcaa
      361 aaaattgatc gagattattt gttcacagct tccagcaaac aaatccgttc ccaaggagga
      421 tctggaagcg ttggttttta tcgagtgtca cccgaaggat cgattacgat gacagatgca
      481 aatggaacac cattttgatt agatgatatt atacggattt acagtactag caagtcatta
      541 ttggtatcat tttttctgag aaagttatac ggagtataaa taagcatttg cattaaccta
      601 ataattcgtt acagctattt ctcttatgaa tatactcact tctctacttg ctctcttgat
      661 aaatttttgg tatctacttc attttcggtc aactgagtta atgaaattta ttgtcgggcg
      721 gttttctaga ttcaattttt ctatatttgg atagaagaat ttactttttt accttttgac
      781 atattttctt cttattattt taatgatccc tttttcaaat tagaaagata gcctttgtat
      841 cgtattagat gaaatattca caaagctaac cattatactt gcattttttt caaaatagag
      901 ttattacgta aatagggata aataaaaaat acttattgaa aggatgatca aaaatggcaa
      961 atgtacctag cattcgcgac atgttccctg atttcaatga cgtgtttagt ccagcattca
     1021 atgatttttt aggtgtttca agttatccaa aagtagattt agtcgaaaat gaaaaagaat
     1081 acaaactaac tgctgatatg cctggctgtg acaaagaaga cacgacagtt gaatattcag
     1141 ataatacttt gacgatctcc gccaatcatg agtcccatac agaagataaa gaagatggca
     1201 actatgtacg gaaagaaaga cattcggtct cttacaaacg ttcattctat cttcctaatg
     1261 ttgatgaaga aaaaatcact ggaaccttta aaaacggtgt gctgaaattg gtattaccta
     1321 aaacagctta tcagccaaaa gaaacaaaaa aaattgagtt aaattagaac ttatcgcctc
     1381 tcgatgaatg aggggcgatt tttatatatt tggataagaa cgagtatgtt taaattttca
     1441 aaatgagcaa aaaaatattt tgtttttacg ataaagatgt tatgatctct cttggtaaaa
     1501 aatatgggag ttgatcattt gattgtctta agtaaacttt tgttttggat accatttatc
     1561 ataatgacca gtgctttctt ttacttattc aaaaggccta acaaatactt actcttatct
     1621 gcttgtccga tgatctattt tatccataag attagaggat tttattatta cgactctggt
     1681 atctcattgt tttgctacga aatcggctta ttagcttcgg ccatcttttt catatttgtg
     1741 ttagaatatt tcagaaaaaa ataaacttcc agtcagtttt ggctggaagt tttttatttg
     1801 gtgcagatct tttcagctgt tattcataca agttgatcca tttttctatt attaagtaca
     1861 taccattgtt actctttgaa tgtgggggag aaaaaaataa aaggagctgg tattttgtta
     1921 gttatgagta aagctttgtt tttacttccc tgcacgttca tgtttattgt tttatgttat
     1981 ttcaaaagaa gaaaaaaatg gtgctggatc gatgttctac tattccttcc ctttttcccg
     2041 cttatttatt ttcttgaaga aatatgggat gatatggcat gtgattctgc ttttcttttt
     2101 agtcattcat tggggcttat gat
//
LOCUS       DAHEYX010000257         2105 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHEYX010000257 DAHEYX010000000
VERSION     DAHEYX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.261"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..104)
                     /locus_tag="MB219_002557"
     CDS             complement(<1..104)
                     /locus_tag="MB219_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCC8428223.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIR"
     gene            496..1518
                     /locus_tag="MB219_002558"
     CDS             496..1518
                     /locus_tag="MB219_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCC8428224.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            complement(1643..2077)
                     /locus_tag="MB219_002559"
     CDS             complement(1643..2077)
                     /locus_tag="MB219_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCC8428225.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
BASE COUNT          682 a          377 c          414 g          632 t
ORIGIN      
        1 cttcttatct caaacattga aggagacaag tatttacgcg cgtagccttc tgctatttga
       61 ctgcggcatg aattgcccgt acataagaaa tagatttttt tcatttgatt tcctccgtta
      121 tggattagta aatgtcactg tttatctaaa gtaaactcta tgtgatggag cgaaaaaaca
      181 atgggtttct tagtttatat atttcttttt gttcagcgta taaatcgatg tgtacaaaga
      241 actaatcttg tcttttttac cataaataca tgttagaaac aatcattttg agtcaaaaat
      301 caacgatgta acagactgtt aaaagagatt aagacacggc agatgatgaa gaaagtaggg
      361 gtgtagtaag ccgatacatt tcttgattgt gaggcgagaa caattattcg tcttttaaga
      421 aaaaaagtag tagaatcagt tcatattaaa aataaaaaac cttccatttt tgtaggttta
      481 aaggaggtca ttaccttgaa aaaaatcggc tttttaagct ttggacattg gatggagcaa
      541 ggatcgcttg tgaaatcagc tcaagatgct tatttgcaat cgattgattt agcggtggaa
      601 gcagaaaaaa tcggagtgga cggagcgttt ttccgcgtac atcattttgc ccctcagatt
      661 gggtcgccgt ttcctttgct agcggcaatc ggcgcaaaga caagtaagat tgaaatcggt
      721 acaggactta ttgatatgcg atatgaaaac ccttattata tggcagaaaa tgccggattg
      781 acagatatca tcacgcaagg aagagtgcaa ctaggagtgg ggcgtggttc acctgaacaa
      841 gttctagatg gctggaaata ctttggttat gattacagtg aagaagaaat caaagaggta
      901 acacgggaac gcacccttga attcttggaa ttacttgaag gaaaaccatt tgaagaacca
      961 aatcctcaac caatgttccc acaaggatca ggaaaattac ggatagaacc ttattcagaa
     1021 ggattacgac aaagaatctg gtggggagct ggttcgcagc aaacggcaat ctgggcagca
     1081 caacatggaa tgaatctgca atcatctaca ttagtcaatt acgaatccca cgagccattc
     1141 catatccaac aagcaaacca attacgtgcg tataaagaag cgtggaagca agcaggtcat
     1201 gattttgaac caagaacatt agttactcgc tcgattcagc caatcaccac tgatttggat
     1261 cgacgcttat ttggacaaga cggtagtccg caacaagatg ctgtcggcta tcttgcttat
     1321 caccgaaacc caacgatttt tggaaaaact tttgctggtg aacctgatca attaatcaag
     1381 gagctagctg aagatgaagc aatcaaagaa gcagacacag tacttgtcac gattcctaat
     1441 actttggggg tagaatataa catgcacttg cttgaaacaa tcgtaaaaga tatcgctcca
     1501 gaattaggat ggcggtaaaa aggatatttt tgtaagtgca ggatacttga gtgaaaaaat
     1561 aaacacttct aacaatcagt tagaggtgtt tattcatgtt ttcatgaagt tcatagagaa
     1621 ggagagtgag ttatccgttt gttcaatgag aatgtacata agcttctttt acatgttggg
     1681 gtaaatcatc aggaatcatt gtcaatacgg cttcttcatt gccggctttt attgcttctt
     1741 catagtacca gcttttgtag tttagcatag cgagtactct ttctaatttt gctatttctt
     1801 tttctactac ttctttttgt ttttcaaata gttctttacg aacctcaaat gtcttactgc
     1861 cttctttgct ccattccatg aattgcttaa tgtcttttat ttctaaacca gattttttca
     1921 aacagtcaat gactcttaaa gcctctaact cattctctga aaattttcta ataccagaaa
     1981 cacgctggat atcaggaaat aatccctctt tgtcgtaata acgtaaagta gaaacaggca
     2041 atccaaacat ttcagacact tgtccaatgg aatacataaa aaaatcctcc taaaataaaa
     2101 ttttt
//
LOCUS       DAHEYX010000258         2103 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHEYX010000258 DAHEYX010000000
VERSION     DAHEYX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.262"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..507
                     /locus_tag="MB219_002560"
     CDS             <1..507
                     /locus_tag="MB219_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCC8428226.1"
                     /translation="INYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNS
                     IEQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFL
                     FLKSILEDSFEYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREIT
                     ARLISKLQ"
     gene            562..1728
                     /locus_tag="MB219_002561"
     CDS             562..1728
                     /locus_tag="MB219_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCC8428227.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          792 a          337 c          334 g          640 t
ORIGIN      
        1 attaactata caactcttac gaactattac cgtggtgagc gaaatcctag aaatactgaa
       61 acatggaata tccttgctga ttatttccat gttcctgtaa gttatttgat gggattagac
      121 gaaacaaatt ccatagaaca acctaatctt gatagaataa aaactgctcg tttagaaaaa
      181 aaggacaaaa tagtcaagaa tattgccagt cttgactcag attattttac tgaattatct
      241 aatctatact tttccatgac ttctttactt gaagtcaaac ttcaagaaga tgactccgac
      301 tttctttttt taaaaagtat tctagaggac tcctttgaat acacgattct atttgccaaa
      361 caaaagaagt ttattaataa agctttaaac ttaaatatcg atgaaagaaa agagttagaa
      421 tctgagttat ttggcaactt aacccaagta caggttaaca atctaaaaag agagataaca
      481 gctcgattaa tatcaaaact tcaataaaac taattattca atcagccaac tcgccaagtt
      541 atcagattgg agagattagt tatgtcaata caaaagtaca caacaaaaag cggtgcacga
      601 tatagtgtcc gcttgtatgt tcgtaaagat gaaaatggaa agcaagaata ctatgtaaaa
      661 aaagggttta aaactgaaaa agaagccaag ttacacgaag caagaaaaaa ggtcgagatt
      721 gaagataccg gctatcataa gccttctaat gacttgttta taacagtgta tgaagaatgg
      781 ttgccttctt ataggaatcg tgtacaagaa acgacatatc aacgtacaaa agaccttttc
      841 cgcttgcata tactaccaaa attcgggaat aagtccattt ctaagattac tcctgttcac
      901 tgtcagaatg cagtcaatga atgggcttta aaattcaaga atatcaaaca gcttaagtct
      961 tacagctctc aaatatttga gtatgccatt tttgcagagc tattacaccg aaatcctatg
     1021 gcaaatacaa atctaccaaa gcgggaaaag aaagaaaatg aaaattactt ttcattagaa
     1081 gaactaaaag ctttttggga tattctcaat aaagaagagc ctttaaaaca tattttaatc
     1141 ttccagcttt tgattactac tggtatacgt aaaggagagc tagctgctct tcattggtct
     1201 gacatttatt ttgaagaaca gctaatgtat gttagaaaga gctatgcaac tattcgaaat
     1261 gaagagaaga atgcaagaag aaaaactgtc aggattcaaa aaaatacaaa aaatatatct
     1321 tccgaacgaa tattaccaat tgatgcacaa actattgaaa tgttaaaaat ctggaaaaaa
     1381 gaacaagctc gagagctttt gcagtttggc ataaatacaa atacaaaaaa tcaacttatc
     1441 tttacctatg taaatgcaga cggtgacatt aatcaacctt tgcatgcgga ctattcaaat
     1501 aatatattga aacgtttaga gaagaaatat aaatttaagc acgttactat tcatggattc
     1561 cggcacactc acgctactct tctactagaa ggtggagcaa gtatcaaaga aacacaagat
     1621 cgattgggtc ataaaaatgc tgaaactacc cttaacactt attctcatgt tactgaaaaa
     1681 gctcaacgaa atgcgttaga aaaattcatt gcttacactg gtttataata ttgtcattac
     1741 ctttctcatt accagaacga aaaaaagaca ccttctcgca atgagaaagt gtctgaaatc
     1801 gttggtataa caacgattaa cgttttgaaa actgtgaagc tttacgtgct ttcttaagac
     1861 ctggtttttt cttcgtatca ttgaataagt ttgaaaagcc ttgatttata aaggtttttc
     1921 cgtaaatata ttttgttata tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc
     1981 taatttaaag gcagtatatt tcgtatttta tattaaattt ttaggctcat tcattctcaa
     2041 atccattcta tttagtcaat aaatatttta aaaagacttt ataaacaaag atgttcaagc
     2101 gag
//
LOCUS       DAHEYX010000259         2103 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHEYX010000259 DAHEYX010000000
VERSION     DAHEYX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.263"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            169..1539
                     /locus_tag="MB219_002562"
     CDS             169..1539
                     /locus_tag="MB219_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCC8428228.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="MB219_002563"
     CDS             1560..1844
                     /locus_tag="MB219_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428229.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cat
//
LOCUS       DAHEYX010000260         2062 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHEYX010000260 DAHEYX010000000
VERSION     DAHEYX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.265"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(25..951)
                     /gene="cysK"
                     /locus_tag="MB219_002564"
     CDS             complement(25..951)
                     /gene="cysK"
                     /locus_tag="MB219_002564"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCC8428230.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1091..1978)
                     /locus_tag="MB219_002565"
     CDS             complement(1091..1978)
                     /locus_tag="MB219_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCC8428231.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          569 a          466 c          352 g          675 t
ORIGIN      
        1 ctatctggag aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg
       61 ttcaccattg tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac
      121 ttttagcgcg gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg
      181 cccaacttct ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata
      241 cacagaagta tccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg
      301 tcccgcttct ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat
      361 tttcggatac acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat
      421 acctgcaaca aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt
      481 cgtcttctca tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc
      541 gttttctttt gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg
      601 agtcaaaatc aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt
      661 ctcaggcatc acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat
      721 tcctgtattt ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg
      781 ttctgctttt tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa
      841 aaactcaagc tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac
      901 gattggcgtt ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc
      961 ctccttgtct ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat
     1021 tcgtctttga ctagcaaaaa aaagacaaag atataccata tatggtaatt tctttgcctt
     1081 tttatctaaa tcaaacgttt agttcttgac caactaataa agaataactg gacatagaca
     1141 tgccattttt cgccattaga tcatcaacag acatgccaaa tttttgtgca atatcccata
     1201 gcgtatcacc tgacacgact gtatatgtct gaccattatt tgtatttaca tctgttgttg
     1261 aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt tgtacttgtg gttgttgtag
     1321 ccgaacctgt tgatggcgta tcaaaacggg ttaaattata agtattaatc aatgagttca
     1381 atttggcccc ataatttgga tcggtagcat aacgacctgt caagtacgct gttgcatcat
     1441 aaaaacttgt tgtattgctt ttccacacgc cagcataatg ataatcgccc gtggaaaaac
     1501 tggttgaacg aagaacattc gcatggtctt ggaaggattc ccaataagaa gaatattttc
     1561 ggaatggctc attcatcgtt acccattgtc cgtttacata ttctttggtt gccatataaa
     1621 cgacctgtcc gttatagctt cctttcacac cgaatagatt ataatttggc gcactagcta
     1681 atccggaagt gccatatgca ctttccaaca acgcttgagc gatcatgact gaagcataca
     1741 agtcattact tgcagcaact tctgacgcag accagccgat ttgatcgata aacgcttgag
     1801 gatcagcgac tgtttgagat ccttgttcat ctgcatgaac atgttgcggt accgctgtaa
     1861 ccatcatcgg cccaactaaa agactcgttc caattaaagg agcaatcgct tttttattga
     1921 tcaccatttt tcttttaggt ctatggtgcc gggcactgcg ggtcttcagc tcttgcattc
     1981 aaattcctcc tcaaaataaa aaaacttaat aaaaagttct cttcttaaaa caaacttaat
     2041 taattaaatt atacttttaa tt
//
LOCUS       DAHEYX010000261         1992 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHEYX010000261 DAHEYX010000000
VERSION     DAHEYX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.267"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(61..354)
                     /gene="rpmA"
                     /locus_tag="MB219_002566"
     CDS             complement(61..354)
                     /gene="rpmA"
                     /locus_tag="MB219_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCC8428232.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(367..711)
                     /locus_tag="MB219_002567"
     CDS             complement(367..711)
                     /locus_tag="MB219_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCC8428233.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(738..1046)
                     /gene="rplU"
                     /locus_tag="MB219_002568"
     CDS             complement(738..1046)
                     /gene="rplU"
                     /locus_tag="MB219_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCC8428234.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1066..1142)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1328..1969
                     /locus_tag="MB219_002569"
     CDS             1328..1969
                     /locus_tag="MB219_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCC8428235.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
BASE COUNT          602 a          436 c          351 g          603 t
ORIGIN      
        1 tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca taagattcaa
       61 ttaagcttct tgagctactg gataaacaga cacttgtttt ttgttacggc ctttacgttc
      121 gaaacgtact acgccatcaa cttttgcaaa taaagtgtcg tctccaccga tacccacgtt
      181 tacacctgga tagatttttg taccgcgttg acggtaaaga attgatccac ctgtaactgt
      241 ttgtccatca gcacgtttag cacctagacg ttttgattct gagtcacggc cgttagatgt
      301 agaaccgcca ccttttttgt gggcaaataa ttgtagattc atagataaca acatggtctg
      361 cacctcctat ttatcattaa ttgttttaat ttggatatat tctgagtttt cagcttctat
      421 cgattgtaat cctaataaaa gattttctaa tagaatctca gcaatattgt tttgttcttg
      481 tgtcattcct gaagtgactt cggtataaag ataaccgcct tcttcttcat tcatttcaac
      541 aatcggctca aaaccggcta atgaggcaat cccgttgaca gtgctgatcg ctaacgcaga
      601 aacacccgca caaacgatat cacttccata aggaccagca tcagcatgcc cggtcaaagt
      661 gaatgaaacg atctgacctg cgtcatttcg cttaaatgtt cctttgatca tatataggcc
      721 ttctttctca aacgctatta agcgttgatc gcgtcgataa ccacttttgt atatggttga
      781 cgatgacctt gtttacggtg tgaatgtttt ttaggtttgt atttgaaagt aacgactttc
      841 ttttgtttgc cgtgtttttc aacagtacct tcaacagttg cacctgcaac agttggagcg
      901 cctacttttg tagattctcc gcctactaag ataacttcgt caaaaacaac tttttctcct
      961 gcttcaacgt caagtttttc aacatagatt gcttgtccca cttcaacttt tacttgttta
     1021 ccaccagttt taataattgc gtacatgcta taaagcacct ccttctgata tacttagact
     1081 cgccatccga agtgacagca tatgctgtgc ttattacttg ttctgagcgg ttgtagctgt
     1141 ggagtccaca attacaacag tactagctta ccagaacagc aaccgttagt caactaaatt
     1201 taaaatcaaa aagtaaaaca aaaattcaag caatacttct gcttttctat cctcacagtt
     1261 ctaccaaata aaatagtcgc attgtgtttc ttttattctt ataataaaag aaaaaggagg
     1321 agcataaatg gaatacccaa aagaactgat acaagaagct tctttaagaa tgaacggccg
     1381 tcctgtagaa ggatttatcg ctgggcaagg acctcttcat cctaaactga tgctcgtggg
     1441 agaagcccca gggaaaacag agattgatac gcatgttcct tttagcggtc aagctggaaa
     1501 agaattgatg caggcacttt cttcaactgg cttaacacga gaagaggtgt atatcaccag
     1561 tgctgtaagg agccgcccgt atcgcgtgac tcatcggatc aataaacgga cacaacagtc
     1621 agaaactgtt tatccaaatc ggacacctac tcgttccgaa gtatttgcgc atgcaccaat
     1681 attggattac gaactgatga aggtccagcc aaaattaatt gcgacacttg gaaacattgg
     1741 tcttcaacgt ttacttggca aagaatatac ggttaccaaa aatcatggtc aactctatac
     1801 agggcctgtc ttacaattaa cagaacaaaa ggatgcctac gaatggtcag aaaaaaacta
     1861 tcgaattctt ccactttttc atccagcagc tattttctac aatcgctccc ttgtacctgt
     1921 tatccatcaa gactggcaag tactagcttc acttttacag caatattaaa aagaacagac
     1981 aagaccataa aa
//
LOCUS       DAHEYX010000262         1974 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHEYX010000262 DAHEYX010000000
VERSION     DAHEYX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.268"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..820
                     /locus_tag="MB219_002570"
     CDS             <1..820
                     /locus_tag="MB219_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428236.1"
                     /translation="VDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPGINI
                     LSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKVSNS
                     DLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLSKGE
                     LDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEYNKK
                     HKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1093..1863)
                     /locus_tag="MB219_002571"
     CDS             complement(1093..1863)
                     /locus_tag="MB219_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCC8428237.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
BASE COUNT          652 a          309 c          348 g          665 t
ORIGIN      
        1 agtagatgga gcattactaa aagaataccg aaagaaaaag gggttaacac aagtacaatt
       61 agcagaaggc atttgtgaac aagcaactgt cagcaatatc gaaaacaaga atatgtcgcc
      121 aggcatcaat atcttaagtg cgctttgcaa aaagctgaat gtcgagatct ccgatatatt
      181 agtagaaaca gaagaagaca aaatcaggaa aaaaataaat gagattcaat cattgaattc
      241 tgtatcaaaa taccaagaat cttataaaat tttacaaacg atcgatcagg aaaaagtaag
      301 taattctgat cttttaaatc aaattttatt ttatcaagga ctgcttgatc atctttatgc
      361 gaaaaaacca gatgcagcat tattttattt taatcaagtg ttggaacaaa cgaccgaaac
      421 agatatccat cacttacaag cagctgcaaa cgtggcaatg atctacttgt caaaaggaga
      481 attagatttt gcgaaagttt atgtggagcg tacacttaaa atattgagtg agactgattt
      541 tgataatctg atggtctgca ttgtatatta tgatatcgcc acctattacc gtaaaatcga
      601 ggattatgat aaagcaatcc aattatgtga gaagggtatt gagtataata agaaacataa
      661 atcgacttat gcattggaat acttgcttta tgaaattgct tcttgccata agcaattagg
      721 agaagatgat tatttagaaa gatatatgga cgcaaaaaaa atagcgcgtt tcaacggaaa
      781 tgattacgcg gtaaaggtga ttgaaaacga cctgaaataa agtggttgtg aaatcagtgt
      841 tctgatttga ataccgttta ttcgaaaaaa ggaggtgtga cttttgtcat acctcctttt
      901 ttcgatattg tttgattcct atttaggcaa catataacgg ttataaaaga agcaatagat
      961 tatgatggta tcgattggat ggatacatct tgggattcct ttatcaattt gagcgtagcg
     1021 tgtttttcat ttatcgataa atttgttatt catttttttg ataatcctat ttacatattt
     1081 acgtcctggt tcttaaaatg aatctacttg aatggttttt atccccgtat ttctgatctc
     1141 ctctaatact ttttcagaac aaaatttatc agtgatcaaa taatcaaaga ctgaaatatc
     1201 agcaatcttg aactttgatg tcagtccgat ttttcgatga tcagcgacta aaatcttaca
     1261 tttgattgtc ttgttgacca tcatttcgtt gatcgttgat tcatttaata tctttgttgt
     1321 gaccccatgt actacatcga ctccgctaca gccaatgata caaatgtctg cattgactga
     1381 agaaatcgta tttaatgcga tgtcgcccac tagtgcttct tttggaaagc gcatctctcc
     1441 accagttaaa atataactac aattatcttt gtgcggagag taattgattt tcacgttgtt
     1501 tgtaatgact gtcaaatggt tagattgaag aaaatccagt gttttcaacg cagtcgagct
     1561 agaattgata aacagcgtat cataatcgtt aatgtattta ctagcttctc tggcaatcgc
     1621 ttgtttaatc ctttcaggac cagattgatc aaaatcagtc tggtttttgt aattataaga
     1681 acaataaccg taattgcgta tgatctcgtt tttttcttct aataaattta aatctcttcg
     1741 tatcgtacta atagatacat caagggattc agctaattcg aaagttgata gttgttcagt
     1801 ctgctgaagc agttccaaaa tttctttttg acgtttttca atattgacaa gtgagttttt
     1861 catgactttt gcctcctgag ggacatcttg atccatggat ttatttgact agttgttcat
     1921 ttaaacattc atatctgtct ttattatact gaaacaatca ttgattactg aaag
//
LOCUS       DAHEYX010000263         1956 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHEYX010000263 DAHEYX010000000
VERSION     DAHEYX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.269"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            571..903
                     /locus_tag="MB219_002572"
     CDS             571..903
                     /locus_tag="MB219_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCC8428238.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1522..1863
                     /locus_tag="MB219_002573"
     CDS             1522..1863
                     /locus_tag="MB219_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCC8428239.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          680 a          264 c          417 g          595 t
ORIGIN      
        1 gagagataat ttcattattt aaacaagcta aaaatcttga tattacttct tttcaggcaa
       61 tacgaatatt attcgcttac attttttgta taaatttaat tgtctgctat gcttgtgatg
      121 aattgatttt cgttctgcac ttttggatga taaatttgga caattgaaaa tacaaaaaaa
      181 agaaaggcgg tatgaactcg gaaggctggt aaccttcgtc agcgttcata cacggcatta
      241 agatttggcc cttaatgcgt taacctttca gtgttacggt attacccgca atggatttag
      301 tataacagat attcaaaagt gataccactt tttttggctg tgttaaggtt aaaaatacgt
      361 gatattacga tgaatctatg cctaagcaca atgttataaa aatgtccgtg tggctctttg
      421 ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg
      481 tttgtcatga acgaaaatca attctctatt tttgaaaaat aacaagaaaa acttgttaaa
      541 aatatttaaa gaaattgggt ggtttatttt atgtcatata caggaatttt gagtctcaaa
      601 gatatttgtc actacggtaa acggtgtacc gctacagaga aaatcacgaa gaaactatca
      661 actggccaaa gtaaaaccgt tgtgcagtgt aagaaatata taatccagaa agacaaagtc
      721 tcagaggaaa tgatttatta cattgggaaa caaaagcaaa taattttaaa agatcctatc
      781 cccttaaaag agctatatcc aacaatcaaa catgtttatg accaaaacgg tgtactgatt
      841 ggtcgaagaa aaaatggagt cttgcgttgt accgcaaaag ggatggggcg tttgatcggc
      901 tgaattgatc attctcataa cttgtatttt atcgtttttc gcttgcttga aaatattgtc
      961 gagacttcta aaggaactta gagtcactag aaaaataagg agtagacttg atctatcaac
     1021 aattcccttc ggggagtgac ggggtaggtt ttaacgattg gtaacgtaaa aaattggtca
     1081 atcgagtaaa tgcgcttgtt tggatagtgg tgaaatgaat ttgtgtaaat aataagcgca
     1141 gagtaaacaa gagggaattt agaagttaga caagttagaa tagcgtctag attaatcatg
     1201 agcagctact aagggatcta gactttgacg caaactcatg gaataaggag taggtcttct
     1261 cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg cagtacatgt agatacttgc
     1321 gaggacgttg attattggat caatcgagca tttaccgctg tctactaggg tttctcgttt
     1381 cggacgccat aaacgagtcg aaatttttta tgtattttta agtaaacgaa aaacgataaa
     1441 atatgaacaa gtcctgaata aagtgaaaaa attgatataa aacatagaga aaatgaaatt
     1501 tacgtaatgg aggaatgaca aatgaaaaat tatgatccga atatacgatt gggcacacat
     1561 acgattaagg tttctttcca acgttgggat tacaaaggat ttgtgacgtt tagaagaggc
     1621 ggaaattgta aaggattaga tgttttagca ttagacgagg atgatttata cgatcaaaca
     1681 ttgactgata atccaattgg attcggcttg ctacctgaag atgatgaagg taatgaatgg
     1741 tttaaaatga cgcttatgaa tgataatgga gacgaattat ctgttgaaga tacttggtca
     1801 tatttgagtg attatattgt cagctttgaa atcattgaat ttgtagcgga taaagaagaa
     1861 taaaaactgg aggaataaat tttacagata aaggcttaga aggagtcacg atgattaata
     1921 aaaaggtagt attaccttag gcattgagta aggaat
//
LOCUS       DAHEYX010000264         1950 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHEYX010000264 DAHEYX010000000
VERSION     DAHEYX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.270"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..159)
                     /locus_tag="MB219_002574"
     CDS             complement(<1..159)
                     /locus_tag="MB219_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428240.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINA"
     gene            complement(490..723)
                     /locus_tag="MB219_002575"
     CDS             complement(490..723)
                     /locus_tag="MB219_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCC8428241.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(738..1685)
                     /locus_tag="MB219_002576"
     CDS             complement(738..1685)
                     /locus_tag="MB219_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCC8428242.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1685..>1950)
                     /locus_tag="MB219_002577"
     CDS             complement(1685..>1950)
                     /locus_tag="MB219_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCC8428243.1"
                     /translation="QSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGACSSLLG
                     LFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
BASE COUNT          594 a          373 c          251 g          732 t
ORIGIN      
        1 cgcattaata tgcggctgaa taatttcttc aaatagcgct tccttagaag taaaatgttt
       61 ataaaaagca cccgtagtta cgcctgcggt caagcacagt tgccttatct tagtttgctg
      121 aaatcctact ttttcaaaat ggcttagtcc actttctatt aatttttggt gagttgtaat
      181 ataatctctg gtttctatgg gtacttcctc ctaataagat gaattaacta tattatctta
      241 cacaggactt gaagtttcaa tcgttaacct ctattactga actcattcct tagagctaaa
      301 ctaatgagaa agagcaaaat aactgataca aaaagcaatc ctacaagaat taatagactc
      361 atctaaatca ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag
      421 ctattaggat aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa
      481 taaagccttt tacttaaaac cttttttgta atcgtcaata aattttttcc ctttatcagt
      541 taattgaaat cctatatcta aatagtcctc atatctaagt ttagtcagca tctcacttac
      601 agatgctgga ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct
      661 ttttgataat tcctgaattg ctagtaaata aattagtata gtagttgttt taatttgtac
      721 catacctacc ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc
      781 atcatcgcat agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc
      841 gttgaataga taggaatatt ggtatctttt gatactgttt tcattggtct atcgtccaca
      901 ctgctttcta cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt
      961 atttgatctg gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca
     1021 ttatacgctt tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca
     1081 ttcggaattg aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata
     1141 tacttatcta gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt
     1201 ttttcaatat ttttagcgta aataataccg ttttctaaat ttaaccaagc atggggatct
     1261 tccttccctt tctcactctg accctctaag taaataacat ctatgccatc acttgctgca
     1321 aaatagtctt tgttttcctc tttgttcgca ttttttacta attttgtaaa ccaagcattt
     1381 cctccagttt ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga
     1441 acatcttcag gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt
     1501 aaatcaattt tatcttttgc tatattttca gtaatatctg ctaaaatcga attagtaact
     1561 actaccttta atttttcgtt cgaagccact gtatctttgt ctttactatt tgttgaacat
     1621 gccccgaata taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt
     1681 ctcatcataa ctttctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac
     1741 taattacaaa taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac
     1801 tataaccaat gaacaaacct agcaatgatg agcacgctcc gactgtcgcc gaaataaata
     1861 tcatagtttt taaactgttt gcatataaat atgctgtagc agctggtgta attaacattg
     1921 caacaattaa tatagttccc acactttgca
//
LOCUS       DAHEYX010000265         1939 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHEYX010000265 DAHEYX010000000
VERSION     DAHEYX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.273"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(241..855)
                     /locus_tag="MB219_002578"
     CDS             complement(241..855)
                     /locus_tag="MB219_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCC8428244.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1461..1838)
                     /locus_tag="MB219_002579"
     CDS             complement(1461..1838)
                     /locus_tag="MB219_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428245.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          638 a          387 c          294 g          620 t
ORIGIN      
        1 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
       61 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
      121 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
      181 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
      241 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
      301 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
      361 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
      421 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
      481 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
      541 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
      601 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
      661 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
      721 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
      781 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
      841 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
      901 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
      961 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
     1021 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
     1081 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
     1141 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
     1201 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
     1261 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
     1321 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
     1381 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
     1441 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
     1501 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
     1561 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
     1621 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
     1681 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
     1741 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
     1801 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
     1861 taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc
     1921 ttatgtaatc attttcttt
//
LOCUS       DAHEYX010000266         1937 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHEYX010000266 DAHEYX010000000
VERSION     DAHEYX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.274"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="MB219_002580"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="MB219_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCC8428246.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="MB219_002581"
     CDS             852..1040
                     /locus_tag="MB219_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCC8428247.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="MB219_002582"
     CDS             1053..1610
                     /locus_tag="MB219_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCC8428248.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="MB219_002583"
     CDS             1642..1884
                     /locus_tag="MB219_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCC8428249.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          713 a          298 c          399 g          527 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtag
//
LOCUS       DAHEYX010000267         1928 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHEYX010000267 DAHEYX010000000
VERSION     DAHEYX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.275"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..367
                     /locus_tag="MB219_002584"
     CDS             <1..367
                     /locus_tag="MB219_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCC8428250.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="MB219_002585"
     CDS             567..770
                     /locus_tag="MB219_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428251.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1928
                     /locus_tag="MB219_002586"
     CDS             1330..>1928
                     /locus_tag="MB219_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCC8428252.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          625 a          293 c          454 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DAHEYX010000268         1868 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHEYX010000268 DAHEYX010000000
VERSION     DAHEYX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.276"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            180..1793
                     /locus_tag="MB219_002587"
     CDS             180..1793
                     /locus_tag="MB219_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCC8428253.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          595 a          340 c          382 g          551 t
ORIGIN      
        1 gggttatccc cttcaaagta ttatctatcc ttaaaataga aaaaaatttt taggttttat
       61 ccacaaatta tttctctata ttttctgtct attcaaaata aacaataatt ttccggcaca
      121 attatttgct gttttttacc ataacatagt acgatcaacg tagaaaagga gatgttttta
      181 tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc atttgccact cgtttccgtc
      241 gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt tcttatgcaa
      301 gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg ctgatcgaac
      361 gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa cacgaagtaa
      421 cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca aatgaagaaa
      481 taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat tatccggata
      541 ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg gatgcggatc
      601 gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt ggtgcaggtg
      661 ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg actttaatcg
      721 atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta gtttatgaca
      781 aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag atccgtgata
      841 aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg ctaatttttg
      901 ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa ttatcagata
      961 caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc tatgcgattg
     1021 gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt atgctttcca
     1081 gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg cctatgcgct
     1141 atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca agttatgcgg
     1201 gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact gtttttatca
     1261 ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta aagctgattt
     1321 ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa ggtgtcgata
     1381 aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt gatttgcctg
     1441 atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg aatatagctg
     1501 gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca agtgatatac
     1561 ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat aaagcacctt
     1621 ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat cgaatcaatg
     1681 aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat acttatactt
     1741 ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa taaaaaaacg
     1801 accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa aatgaaaaag
     1861 tatatagg
//
LOCUS       DAHEYX010000269         1850 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHEYX010000269 DAHEYX010000000
VERSION     DAHEYX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.277"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            343..1161
                     /locus_tag="MB219_002588"
     CDS             343..1161
                     /locus_tag="MB219_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCC8428254.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1234..1593)
                     /locus_tag="MB219_002589"
     CDS             complement(1234..1593)
                     /locus_tag="MB219_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCC8428255.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          611 a          286 c          343 g          610 t
ORIGIN      
        1 gttatgaatt atccagatca aatttgtgta ttacattcag taataaggtg cttatcttag
       61 gatcaactta tgatactagc tgaccaagga tgtaccagtt atagaaaatc agttaaagta
      121 aaaatgataa aaatcttatg tcaacactga tttaggctga cctgtgggtt aggagaaaga
      181 caggtcggta gcttttaata caaaaaatgg cttgtatgta tctagatagt taactgaaat
      241 tagaaatgtt cttattttta acggagtaag aaaaataggc tgctttaggt ctgaatactt
      301 tttcaatata atgaagatat caaaggcgac aggagtgttt ttatgaataa gaatcctcat
      361 aatctaaagt tggtagcagt agatatggat ggaacatttg ctcgtactga ttacacatac
      421 gatatcccaa ggttcaaggc tgttttatcg cgaatgaaag ctgttgattg tcaatttgtt
      481 gttgcaagtg gaaatcaata ctaccaattg agatcgttat ttccaaatta ttatgatgaa
      541 ttaagttttg ttgctgaaaa tggtgctttt gttaaagata aaacagaggt actattttca
      601 gccgatatgc ctagaaaagc gatcgaggtg gtccttgatt tatgtaaaga ctatcctgaa
      661 gtaaaaaatg tgatgtgtgg tctgaatagt gcctactgtg aaagaggaaa ggtgagtcaa
      721 gcattttttg atctaaccaa tatctattac catagattag aatgggtagc tgattttgca
      781 gaagttcaag ataaaatttt aaaatttgct ccgactatgc ctgttgaaaa aaccgaattt
      841 tacgttgaat tgttccgtga aaaattagcc gggctattag tacctaccag tagtggtcat
      901 ggttctgtag acttgattct ttctgagtgc cataaaactt tcggcttgaa aatgttagtt
      961 gagcgcttgg ggattaatcc tgaccagtgc gttgcttttg gagatggggg caatgacatt
     1021 gagatgttgg agtattgtgg tctgagttat gaaatggata atgcaacgga agctgttaag
     1081 caagttgcta agcatcaatg tccttcaaat gacgaagacg gtgttttagt gacgctcgat
     1141 cgattgttcc ctaacacgta aacactaggt aattcatcaa aaaacctcga tataagaaat
     1201 attcgaggtt ttaaaactat atttagaaca gatttacgaa gcgtctgatg agtcaaaata
     1261 gacttggaca ttgctatcta aaataccact ttcattcttt aaacgaattg tttgatttga
     1321 tggagttacc tctaatacta aatgcccatt aataaaggta gtatcgtatt cagatgtttt
     1381 aactgttgct tcttcgcaag gtattgttaa atcatatgct ggaatataat gttgtgtgga
     1441 aactaataaa aaatgatcac caatcataca gcatccctct gctgaagaaa attgattaga
     1501 gccatcattt aaagttaaca agaatccatc ctttaaacga ggtacttgct taaatacttc
     1561 tggttgtact tctaaaaata aattatcgct cattttcacg accaccatcg acttccacag
     1621 ttgaaattga cattttattt aacacaccta ttccccctta actataactc aattctatat
     1681 aaaaaatatt ctattctcaa ataattgaac ttttcaaatc gccaatttac ttcggcttta
     1741 tcagagctca ttcatatcaa gagcgattaa cgctgtaaaa attaaaccga agatctgata
     1801 aattttctct attttctaca atatttaaaa cgcttcctta gtctattata
//
LOCUS       DAHEYX010000270         1823 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHEYX010000270 DAHEYX010000000
VERSION     DAHEYX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.278"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..953
                     /locus_tag="MB219_002590"
     CDS             <1..953
                     /locus_tag="MB219_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCC8428256.1"
                     /translation="QEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPKEVSEKKLP
                     AIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVASPDINTEDF
                     QAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYDMTRVTANG
                     YFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKDYNAYYKTD
                     RGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRYFGEDAFKE
                     LKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            complement(1351..>1823)
                     /locus_tag="MB219_002591"
     CDS             complement(1351..>1823)
                     /locus_tag="MB219_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCC8428257.1"
                     /translation="PSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQ
                     KSDIKHHLKTNPLFKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATV
                     GGFAHSLEQNYPLKECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
BASE COUNT          564 a          334 c          382 g          543 t
ORIGIN      
        1 cacaagaatg ggacaaagta tttccaaaaa gcaatcaagt caatcatcga aaggtgagct
       61 tcccgaatcg ttttggtatc acattagtcg ctgatttata tgaaccaaaa gaagtaagtg
      121 aaaagaaatt gccggcaatc gctgtgagcg ggccatttgg tgcagtgaag gaacaatcgt
      181 ctggtctata tgcgcagacg atggctgaaa gaggcttttt aacgattgcg tttgatccat
      241 cgtttacagg agaaagcgga ggagtacctc gatatgttgc ttcgcctgat atcaatacgg
      301 aagatttcca agcggcagta gactttttat ctgtacaaaa aaatgtaaac cctgagagaa
      361 tcggaatcat tggtatttgt ggatggggag gtatggcatt gaatgcagca gctatcgata
      421 cgcgggtcaa agcaacagtt gtttccacaa tgtatgacat gactcgagta acagcaaatg
      481 gctacttcga tgttatggat gaaaatgccc gatatgaaat gcgtgaagaa ttaaatgcac
      541 aacgcatcat tgatttcaaa caaggggact acgcacgtgc tggaggtgtg gttgatccgt
      601 tgcctgaaga tgctcctcaa tttgtcaaag actataatgc gtattataaa acagaccgcg
      661 gatatcatgt ccgttcttta aactccaatg atggttggaa taggacatca gggttgtcac
      721 ttatgaatgc aaaactattg cattatattc cagaaatccg tcatgcagtc ttagtcattc
      781 acggggaaaa agctcattca cgttactttg gggaggacgc tttcaaagaa ctaaaaggtg
      841 acaacaagga actcatgatc attccaggag catcccatac agatctatac gaccagacag
      901 acatcattcc atttgaaaaa atggcaacat tctttgaaga gaaccttgtg taaccaaaag
      961 gatttaatct aaaagagatt atgaaaaaac tgccctgcat caagtaataa ggacagccaa
     1021 aaaagctcct catttttgac ttggttggac tcattagcgt agatgcaagc ttttgaacac
     1081 cgtttatacc aagaggtcgt gagcctgccg tttaactctg agtcacaagg agcaatttca
     1141 aaaaatagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt tggcaggaga
     1201 acaccgttta ttcgaaaaaa ggactgtgac aatgtatcga ctcccaaaag ttagaccaaa
     1261 aaatctaact tttgggggtc ggtacacacg ttttttgtca cagtctcttt gtgtctcatt
     1321 tcatataaac aattatttac tttgtactat ttatttcatt tctgtgattt taatgctttt
     1381 tgctaaattt tttacatcag ctggaataac tttcccgact tctgattgct gtgcattcga
     1441 catgccgcaa gccattgcgt accttacaca ttcttttaaa ggataattct gttctaatga
     1501 atgagcaaaa cctccaacag tggcatcccc agagcctgtt gtattaatga taggaatctt
     1561 agggatctca acattccaaa attcttcttt tactttgact actgcaccat acgagccgca
     1621 agaaatcatg ataacatcga tatctttaaa tagtggattt gtttttagat ggtgttttat
     1681 gtcgcttttt tgagtaattt tttgatggat gatatctgaa aactcatgga tattaggttt
     1741 gatgaacatc ggtttatact tacttgctaa gacttcacgg agatgttgtc cagatatatc
     1801 cattaatata taggtggaag gat
//
LOCUS       DAHEYX010000271         1822 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHEYX010000271 DAHEYX010000000
VERSION     DAHEYX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.279"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            109..579
                     /locus_tag="MB219_002592"
     CDS             109..579
                     /locus_tag="MB219_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCC8428258.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            664..1272
                     /locus_tag="MB219_002593"
     CDS             664..1272
                     /locus_tag="MB219_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCC8428259.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1406..1606
                     /locus_tag="MB219_002594"
     CDS             1406..1606
                     /locus_tag="MB219_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCC8428260.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT          607 a          261 c          367 g          587 t
ORIGIN      
        1 agagacaaag gcaaaggatc aaaaaaaatt aattttttta aataaagatg aaagttattg
       61 aaatatagag tatgataaat aagaattttt aataaggtag gttggctcat gaagcagtat
      121 cgacagttaa aaggatattt agaagcaaaa ggatattggg agatactagt ctatctcttt
      181 tttggtggtt tggctacgat tgtaaatttt gttagttttg ctctcgcacg ccaatatttt
      241 gatttaagta tggccttatc caattctatt tcgtggtttt gttctgtatt gtttgctttt
      301 gtaacgaaca aactttgggt attccattca aaatcaccga attttacaca tgcacttatt
      361 gaatgcggta agttcttctt ctaccgtatc ctttcttacg gattggacat gggggcaatg
      421 gtattgttga tcaatgttat gaacagcaat gagtatgtgg cgaaaatcat cactcaaatt
      481 attgttatcc tagcgaacta tattttcagc aagttgttta tttttaaaga aactgaggtt
      541 tttgaagaag aagtaggtca aagtgaaaaa gacgactagg cataacaatt gaagaatttc
      601 cttctctttg ttactctttg taatgtagac attataaaga aaacattgag gaggaatttt
      661 tttatgactt acacattacc agatctacct tatgcatatg acgcattgga accttatatt
      721 gacgaagaaa cgatgcatct gcatcatgat aagcatcaca atacttatgt gacgaattta
      781 aatgcagcaa ttgagaaata cccagaatta ggcgaaaaaa caatagaaga attattatct
      841 gatatggacg ctattccaac agatatcaag acagctgtac gtaacaatgg tggcggacat
      901 gctaaccatt catttttctg ggaaatcatg gcaccaaatg ctggtggcga acctacagga
      961 gaaataaaag aagcgattaa tgaagctttt ggtgattttt cttcttttaa agaagaattc
     1021 aaaaaagcag ccgctggacg atttggttct ggatgggctt ggcttgtaat ggaaaatgga
     1081 aaattagcta ttacctctac tgcaaatcaa gattctccat tgatggaagg aaagacacca
     1141 attctaggtt tggacgtttg ggaacatgct tattatttga aatataaaaa tgtccgacca
     1201 gattatattg cagcattctg gaatgttatt aattgggatg aagtaaacaa acgtttcgaa
     1261 gcagcaaaat aaccatttcc tccgtcatat cagtcttaaa aataatttgg aaaataaaag
     1321 agcgaaagct tgctagcgtt ttctaaattg ggtataatgt tcttgcaagg ttacttgcaa
     1381 gaactttttt ggaggctata catttatgga acacggaacg gttaaatggt tcaacaatga
     1441 aaaaggattt gggtttatta cagtagacgg cggagatgac gtatttgttc atttctcagc
     1501 aattcaagga gacggcttca agagcctaga agaaggtcaa gaagtagaat tcacgattgt
     1561 tgaaggtgca cgcggacctc aagctgctga agtgactaaa ttataataat ttgttgaata
     1621 aagacacgac ttcttagaga gtcgtgtctt tttgtctatt tatgaagttt ttagattttt
     1681 tggctaagta aatgcatttg ttcacttgac atgtcctaac taattatcaa gcaaggaaca
     1741 ttttcttcgt tagtaatgga ttttaaagaa atttcagttt ttggatgttg aataaaagac
     1801 catttgtgaa accactagta ta
//
LOCUS       DAHEYX010000272         1796 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHEYX010000272 DAHEYX010000000
VERSION     DAHEYX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.280"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            71..880
                     /locus_tag="MB219_002595"
     CDS             71..880
                     /locus_tag="MB219_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCC8428261.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            920..1417
                     /locus_tag="MB219_002596"
     CDS             920..1417
                     /locus_tag="MB219_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCC8428262.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          631 a          315 c          337 g          513 t
ORIGIN      
        1 ttttttaaat tctcatttaa tcttaaggta acaggaaaat agatatgtaa aaagaaagta
       61 ggaagataaa atgaaactag cagcgattga tttagacggt accttgcttg atcgaaatgg
      121 aaagatacca gaagaaaata tccaagcact tcggaaattt gatgaaaatg gcggaatcgt
      181 aaccgtcgct accggaagga acagcatttc tgctaaagat attttttccg aattaggaat
      241 cggtggctat ttgatttctt ccaatggttc actgattgct gaaatgaagg atggaaaaat
      301 ggcgaaagtt ctcagacgtt caaaaataga tagcgataca ctgagaaaag ccttcttcta
      361 tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa acccaagatg atcagctgac
      421 attcgatgaa tcaaatctag tcaaagatga cccttattat gaacatttca atttacagaa
      481 ccattctttt gatcaaattt taaatatgtt ggaagatcct tctcttagct atcttaaatt
      541 agcattaaca gataagagtg aagaaaaact acagaagcta caaagagatc tgcaagcaga
      601 tggtatccaa tcagtattct ctgatccgca tttcttagaa atcacgccta caggtatcac
      661 taaagctcat tcacttgttt ttctggcgga ttaccttgga atcgaatcga gtgacatcat
      721 ggcttttggc gatcaagaaa acgacattgc tatgttgaat tacagcgggt tgagtattgc
      781 gatggggaat gcacagcgac aagtaaaaga aattgcaaac caagtaacac tgacgaatga
      841 agaagctggt gttgcttata ttttaaaaaa cttctattaa aaagaataaa atgacgatat
      901 gaatgtagga ggaaagaaaa tgttagttat tattgatatg caaaatcata ttcttgatcc
      961 aaacagcgaa ttttatattc cagagtcaga tcaattggca gaacgtattg ctcaaagatt
     1021 agctaaagca cgccaaaata atgaatatat tctcttcaca cgggatatac cagttgaact
     1081 gaaggacaaa gaagaagaac gagaagattt gcaattgatc cctcttcttt ctccacaaga
     1141 aaatgaacga gtgattaaaa agtattactt tacgattcca cctgaaactt tagctgaaat
     1201 ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag atcgaagtag tcgggattga
     1261 aacaaacttg tgtgtactct ctaatctgat aggcctgcag agtgcttttc ctgaagcaga
     1321 cttttttgtt gatccaatgt tagtctccag tagaaaacac ggagaatccg ctttagagct
     1381 gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa tatctatctt catttacgat
     1441 atttaacaca caataaaaaa agcacgcaga actgttttag tcctacgtgc tttttctatc
     1501 aaagtagatt ggctttttta tgacacagag tgaaacggca ggctcacgac ctctttctta
     1561 aatggtgttc aatcagctga ccctcggtat tagcttaaca aacggcaaaa tatgaggaac
     1621 tatttttccg tttgttaagc taatactcag gtcaaaacgc tgatttcaca acctcttcct
     1681 taaacggtat tcaaaagctt gcatctgcag taataagaac agccaagtaa aaacataaga
     1741 agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc acaacc
//
LOCUS       DAHEYX010000273         1788 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHEYX010000273 DAHEYX010000000
VERSION     DAHEYX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.281"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..182)
                     /locus_tag="MB219_002597"
     CDS             complement(<1..182)
                     /locus_tag="MB219_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCC8428263.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="MB219_002598"
     CDS             517..951
                     /locus_tag="MB219_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCC8428264.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="MB219_002599"
     CDS             974..1294
                     /locus_tag="MB219_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428265.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="MB219_002600"
     CDS             1435..1626
                     /locus_tag="MB219_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428266.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..>1788)
                     /locus_tag="MB219_002601"
     CDS             complement(1697..>1788)
                     /locus_tag="MB219_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCC8428267.1"
                     /translation="SVLITHIIELTLSVIFLIIWIYFLYKNQN"
BASE COUNT          679 a          266 c          374 g          469 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacg
//
LOCUS       DAHEYX010000274         1786 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHEYX010000274 DAHEYX010000000
VERSION     DAHEYX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.282"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            113..1744
                     /locus_tag="MB219_002602"
     CDS             113..1744
                     /locus_tag="MB219_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCC8428268.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT          516 a          313 c          395 g          562 t
ORIGIN      
        1 aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg
       61 atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa
      121 aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa
      181 agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg
      241 ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt
      301 tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc
      361 tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt
      421 agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat
      481 cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca
      541 gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag
      601 ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat
      661 tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc
      721 tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa
      781 gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc
      841 cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt
      901 gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt
      961 aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc
     1021 ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat
     1081 gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat
     1141 tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga
     1201 aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac
     1261 gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg
     1321 ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg
     1381 gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag
     1441 tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca
     1501 tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc
     1561 agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc
     1621 cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca
     1681 acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca
     1741 ttaaaacaat gaaaaagaac agacaagact gtaaactgaa gatttt
//
LOCUS       DAHEYX010000275         1774 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHEYX010000275 DAHEYX010000000
VERSION     DAHEYX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.283"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            197..424
                     /locus_tag="MB219_002603"
     CDS             197..424
                     /locus_tag="MB219_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCC8428269.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="MB219_002604"
     CDS             547..873
                     /locus_tag="MB219_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCC8428270.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="MB219_002605"
     CDS             941..1654
                     /locus_tag="MB219_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCC8428271.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          374 g          441 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt gttt
//
LOCUS       DAHEYX010000276         1763 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHEYX010000276 DAHEYX010000000
VERSION     DAHEYX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.284"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            11..1350
                     /locus_tag="MB219_002606"
     CDS             join(11..452,452..1350)
                     /locus_tag="MB219_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCC8428272.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            1537..1610
                     /locus_tag="MB219_002607"
     tRNA            1537..1610
                     /locus_tag="MB219_002607"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1571..1573,aa:Ile,seq:cat)
BASE COUNT          698 a          256 c          308 g          501 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag ttcaactttt ggggttcagt tcaatgaggg
     1381 gcggtacata actatattga aaactataaa aatcattcga taaaatagtg atgttgccgc
     1441 atatcttcac tatcactcat aaatagtcac actccaagat atgcgataac aggtttgttg
     1501 ccacacattc tactggttga ctgtttatgg ctttccgtac ccttagctca gttggttaga
     1561 gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acagggtaca tactctaagt
     1621 gatttaaaat gcgaataaaa tataaaaatg actccccttt tgactccccc aagcagtata
     1681 tggatgtctc taaaagttgt ttttttagat aataaaaagc tgataataag cgaaaaacac
     1741 ttaaaatcag ctttaaaccc tat
//
LOCUS       DAHEYX010000277         1750 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHEYX010000277 DAHEYX010000000
VERSION     DAHEYX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.285"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            21..1007
                     /locus_tag="MB219_002608"
     CDS             21..1007
                     /locus_tag="MB219_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCC8428273.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDTLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVVREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHMEDRNKKS"
     gene            complement(1205..>1750)
                     /locus_tag="MB219_002609"
     CDS             complement(1205..>1750)
                     /locus_tag="MB219_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCC8428274.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTATVV"
BASE COUNT          603 a          298 c          376 g          473 t
ORIGIN      
        1 atttgctagg aggaatttac atggaagaga aaaatttaac ggtcatcgga agctatagta
       61 cgagggaaga agcattatca gtaattgaac ggttgcgtaa cgaagggtat gaacgagatg
      121 atatcgttat ttatactact gatgaagcag catctagact aggatttgac ggattgtctg
      181 gtatagatgt tgaaacagat gaaaatcgga tggatggtga agaagaccgt tctttatggg
      241 aaaaaataaa agatactttt tcttttgata cctatgattc tgagacggct acaccagaaa
      301 atgatacttt atatcaatat agaggagata tttctgatgg aaaatttgtc atcacagtaa
      361 aaggctatcg tcagccagaa acaacagaag atacaatgga tacacaagat actgttagcc
      421 cagcaagcac aatggggact ggattagaaa ctggcactgg aacagatcca atggacgtag
      481 gtggcacgac aggctttcaa aatacaacgg atccattgaa tatgacacca ggaactgatc
      541 cgatggacgt gcctggcgag actgatacaa tgggtacaag caagactgaa gacatcgatc
      601 gcaaaccaaa tctagatgat gatacaattc aactaaaaga agaaaaatta gatgtaaata
      661 cacatgatgt gacaactgga gaagtcgaca tccacaaaca tgtggtcaac gatactgaaa
      721 cggtagaagt accggtcaaa cgtgaagaaa tcgtcatcga acgcaaacca gtaacagatc
      781 aatcttcaca gggaacagac gaaaacttgg aagatgacac gattactatc ccaatcaaag
      841 aagagcaagt agatgtaagc aaacatacag ttgttcgtga agaagttggg attcataaag
      901 aagaacatga agatgttgaa aaagtgacag aagatgttag ccgagaagaa ttggatattg
      961 acacaagcgg tgatgtacac atggaagacc gcaataaaaa atcgtaagcg taagctaaag
     1021 aggttgtgac agaagtggtc agcttaaaga aatttcggct tatttccgaa gaagctactt
     1081 ctggaacacc gtttattcgc aacaaaaaag ggctgtgaca agattttttg tagtgacccc
     1141 caaaagttag acttttttgg agtggagaaa tccactcctt ttttcatgct ctaaaatgat
     1201 atttttacac aaccgttgcg gttgttttac gaaattggac gggactttgc cagtttaatt
     1261 tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct gattttaatt
     1321 cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag ccaaaaaaat
     1381 tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga aagattttgt
     1441 actccttcaa tttgtttcta taagctttca tttgatacgc ccaaccttga tctgagtgaa
     1501 acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa ccttccatta
     1561 ccgctaatgc attgggcttt tcagagatac gataggataa aatctcacta ttgaacatat
     1621 ccataaaagg atctagatac aatttctttt gacggataat gcctttggca tccacttcat
     1681 ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg gcagatactg gtgtagaaac
     1741 gacgatggat
//
LOCUS       DAHEYX010000278         1738 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHEYX010000278 DAHEYX010000000
VERSION     DAHEYX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.286"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..899
                     /locus_tag="MB219_002610"
     CDS             <1..899
                     /locus_tag="MB219_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCC8428275.1"
                     /translation="PKTDFKLFFNYCNILKKIKPDMVIAYTIKPNIYGGLACRLRKVS
                     YALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSKVIFFENFENMKTFQTLQIAKQSKY
                     KLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLD
                     VLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPFVERAHCFVLPSWHEGMANTNLESA
                     ASGRPIITSDIPGCKEAVIESKSGYLCERKNSCSLYNSMNKFLKLSYEQRINMGKMGR
                     NYMIKKFNKKKVVDETIKSLFS"
     gene            945..>1738
                     /locus_tag="MB219_002611"
     CDS             945..>1738
                     /locus_tag="MB219_002611"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCC8428276.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPIISAIIPKYIMYRYQ"
BASE COUNT          684 a          188 c          331 g          535 t
ORIGIN      
        1 atccaaaaac tgactttaaa ctatttttta actattgtaa tattttgaaa aaaataaagc
       61 cagatatggt cattgcttat actatcaaac caaatatata cggtggattg gcatgtcggt
      121 taagaaaggt ttcttatgca ttaaatataa ccggattagg aacggcattt caaaaaaagg
      181 gggttttgaa aagtcttatt gtatttttgt atagattagc agctaaaaaa tctaaggtca
      241 tatttttcga aaattttgaa aatatgaaaa catttcagac tttacaaatt gctaaacaaa
      301 gcaaatacaa gctattaaac ggtgcaggag tagatgtaga acactttagt tatttagaat
      361 atcctaaaga tgagcaagtt cattttttat ttattggaag aattatgaaa gagaaaggtg
      421 tagatgaact ttttgaagca atgaaaaact tatatagtga agagaaaaat tgtgtacttg
      481 atgtccttgg gtattttgaa gaagattacg gcataaaaat tgaaaaatac gaaaaagagg
      541 gatggttaaa atatcatgga tatcaatctg atattcgtcc ctttgtagaa agagcacatt
      601 gctttgtttt gcctagttgg cacgaaggaa tggcaaatac taaccttgaa tcagcagcat
      661 cagggagacc aattattaca agtgatatcc cagggtgtaa agaagcagtt attgaatcga
      721 agtctggtta tttatgtgaa agaaaaaata gttgtagtct ctataattcg atgaataaat
      781 ttctaaaatt atcatatgag cagcgcatta acatgggaaa aatggggaga aattatatga
      841 ttaaaaaatt taataagaag aaagttgtcg atgaaacaat taaatcatta tttagttaaa
      901 aagatatgtt ttgcaaagta tgtgatagaa aagagggatt ctgtttgagt aacgtaagta
      961 atccattagt aagtgtaatc atgggagcct acaattcaga gaaaacaatt ggaagagcaa
     1021 ttgattcaat tataaaacaa acatatttac attgggaaat gataatttgc gatgatggat
     1081 caactgatgg aactgtaaaa ataatacgga aatatgtaga aaaggataaa cgtattaagc
     1141 ttgttcaaaa taaaaaaaat agtggattag catattcatt aaatcattgt ttgaaatatt
     1201 catcgggaaa atatgttgca aggatggatg cagatgatat tagtttacca gaaagatttg
     1261 cataccaagt tggattcttt gaaaaaaatc cagaatatga cgttgtagga tgtaatcgaa
     1321 tagtattcga tgaaaatggt gattctggaa tacgaaaatc aaaggaaatt ccaactatta
     1381 atgacatgta taaaggttct ccatttgctc atccgacaat aatgatgaaa cgaagtgttt
     1441 atactattct aggtggttac agggttaaca aagatacaac aagatcagaa gatttagaaa
     1501 tgtggtttcg attcttcaat gaaggattta gaggttataa tttacaacga gtcttattta
     1561 aatatcaaga aacaagcgaa gattttcata aaagaagttt aagagcagct tacggaataa
     1621 caaaagtatt gttgagaggt tataagttaa taggagttag taaatataaa tatatttacg
     1681 catttaagcc gattatttcc gcaataattc ctaagtatat tatgtataga tatcaaga
//
LOCUS       DAHEYX010000279         1718 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHEYX010000279 DAHEYX010000000
VERSION     DAHEYX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.287"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            250..1542
                     /locus_tag="MB219_002612"
     CDS             250..1542
                     /locus_tag="MB219_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCC8428277.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          551 a          292 c          349 g          526 t
ORIGIN      
        1 ttcttataat gtgtatcaat attaaaactg aaaaagatat ggataatagg atcaaagccc
       61 tcattggaac actcaatata ttatcagatt taaatttcaa atagataatt tcattgttta
      121 gtaatatcga aagatacttt tttaatgatg agagtgtaat ataaaccgtg taagtaacca
      181 ttaatcctag gcaggtggtt gcttcgcggt ttttcttttt ctacaataag atcaagaggt
      241 gattacacga tggctagaaa gaaaagaaat cctgatgccg aaaagttagc tgaatccatt
      301 ctgaatgcct atcaacctga atctgtcgat gacatgcaag atgctttgaa agatgtgttt
      361 gggccccttt ttgaaaaaat gcttcaagga gaattgaata atcatttagg ttatgatgcc
      421 cattctaaag agcctaagga acacgataac cgtcgaaatg gctatggaac taaaacgctt
      481 aaaaccagtt ttggtgaagt agctattgat gttcctagag accgggaagc ttcctttgaa
      541 ccagagttaa ttcctaagag aaaacgagat gtctccgaca tcgaagggaa ggttctttcc
      601 atgtatgcac gaggaatgag tcaacgagat attgccgcaa ccgtcgaagc tatttatggc
      661 tttgatattt cccatgaaat gatttcagat atcactgacg ctgtccttcc tgaattggaa
      721 gaatggcaag cccgccccct agcaaagtgt tatgcctttc tattcgttga ttgtatgtat
      781 gttactttac gagaaaatta tgaagccaaa gaatatgctg tatacaccat tcttggctat
      841 gatctcaaag gaaataagga gattttagga ttatggttaa atcaaacaga atctaaaaat
      901 cgctggatgc aagtatttga tgaactgaaa gcacgtggtg tcgaagatgt ctttttcatt
      961 tcgatggacg gtgtttctgg cctcgaagaa ggcgcaaaag cgatctttcc atcggtaatt
     1021 gttcagcgct gtattgttca tcttgttcga aatgcattgc gctatattcc aagtaaggac
     1081 tataaggaag tctgccgaga tatgaaaaag ttctacggtg cttcttctct aaacgctgca
     1141 catgctgctt ttgacagttt tcaaaatcgg tggtctcatt attctggtgc tgtagatgtc
     1201 tggaaacgga actttgcaca tgttgaacaa ctatttgatt atggtagtgc cattcgaaaa
     1261 attatgtaca ctaccaatgc ggttgaaagt gtccactcca gcttcagaaa agtcacaaaa
     1321 aaaggagcgt tctctaatga gaatgcactc ctaaaactac tttatttacg cacgaaagaa
     1381 ttacacgcga agtggtcagg tggtcgaatt caaaactggg ctatggtgct taatcagtta
     1441 atgatcaacg agaccttctc ctctagaatg aaaagtatgc gatttacctt ccgtaacaac
     1501 ggtaggttta ttcaactatg tgtccgatca aaatttatct gatttcaaaa tagccgagag
     1561 aattatggtg gttaaaaact cttaactatt taattacggt tttcatagtt tttggattca
     1621 ggaggattaa aacgaattct ataaaaaaag atttggttta aatgaatgat taaacgacaa
     1681 tttagcaacg tagtcagagt aaaacagctc tttctttt
//
LOCUS       DAHEYX010000280         1716 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHEYX010000280 DAHEYX010000000
VERSION     DAHEYX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.288"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..125)
                     /locus_tag="MB219_002613"
     CDS             complement(<1..125)
                     /locus_tag="MB219_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCC8428278.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAV"
     gene            283..546
                     /locus_tag="MB219_002614"
     CDS             283..546
                     /locus_tag="MB219_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428279.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            570..758
                     /locus_tag="MB219_002615"
     CDS             570..758
                     /locus_tag="MB219_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428280.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(841..1026)
                     /locus_tag="MB219_002616"
     CDS             complement(841..1026)
                     /locus_tag="MB219_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428281.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1241..1612
                     /locus_tag="MB219_002617"
     CDS             1241..1612
                     /locus_tag="MB219_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCC8428282.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT          633 a          236 c          325 g          522 t
ORIGIN      
        1 tatacagcag gtaaagaggc cagtaaaact gaaaacttct tttttgtcca ttttatgtaa
       61 tagaaaagaa tgcttatcat aataaaaaaa ggaatataaa aaacaaattg accgatcgcg
      121 atcatactat tgcctccgat aaataaagtc catgatacaa aataacactt tgtgcgaaat
      181 ggcgcaagca gttttgtgga tttatccggt aaaatctgta tcgattttga aattagagga
      241 gaaacttgct ataatcaaag tgaaagaagg agtgggattt tcatggatca tgtagtgtta
      301 gaaatcgatt tgcatttgat caataatatt cgggtgattt actatgttgt aagtaacagt
      361 gtcgaacaac gtgtactgac gaataaaata aatggtattt tagcaaagaa agacgttcac
      421 cgattcaata atggtgaagg aagttattat agtatcccag tagaaaaaat tatttatacg
      481 acagtcaaag tcagagaaga tctagatacc aaaaaagcat atgaacctat ttttacaact
      541 tattgattga cagcagatgg aggaaaaaaa tgaatccata tgaacaactt attcaagatt
      601 tgatcgatgg aaaaatcgaa aagattgatg taaaaagaga agaattaatg gcttttcgag
      661 aagcgtggct gaagttggag aatcgtaaat atgtggtggg aaaagcctca ctgaacggca
      721 atgtgactta tcattacgat ccgacacgtt tattttagta tgaagtattc agaaaagaac
      781 caaaggagga cgatgcagca gcattgtcct cctttgattt tacaaaagtg aaagttttac
      841 ttatttgaat agatttttaa atttgttttt gacactgtcg atatttacat cttttaaaag
      901 attttgggct ttctctttat aaggtcctaa ttgttcttta tgttcttcga taaatcgttt
      961 tgcgccatcg aaattgccgt tcttaatcat ttccttgact ttttgaaata attcttcttg
     1021 attcattttg aacacatcct ttgctatcaa catgattttt tacatggaca aaacgagaaa
     1081 aactgattta tccacacgtc aataatatca aataacgatc atttttacta aaaaaacact
     1141 cgattctcgc ttctaaagta ggagaaaaat ctccaacttg ggacggatgg aatttcttta
     1201 actgacccat atactgaata tataacaagg aggtagaaag atgaaagtga ttaaatattt
     1261 gattggactg gcggcgatta gtggtatttt gctatttgga ctgaaatatt atactgaagg
     1321 aagttatgga gaaatccctg gtatacttga tcagctaaat ccattagttg aaaaaggaga
     1381 ggtctatgta aagacacaaa aaccagagga agtaaatgaa tatggtacag caaggtatgt
     1441 gcagaaagct gctgatgcca atgggaaaga acgaacggtt gaatttaatg gtctttctgt
     1501 gttaaaagaa aatcattatt tgaaaattag caataaagga gcacacatag aaacgtatga
     1561 ggaagttcct agagaaaaag ttccggagaa agcattagca attattgggt aaatcagttt
     1621 ttgtattaag aatacgcccg attttattat tcataattta attatgtaaa ctaaatacaa
     1681 gtagtaaaaa taatacaata atccccctgc aaaaag
//
LOCUS       DAHEYX010000281         1708 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHEYX010000281 DAHEYX010000000
VERSION     DAHEYX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.289"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(537..902)
                     /locus_tag="MB219_002618"
     CDS             complement(537..902)
                     /locus_tag="MB219_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428283.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(978..1223)
                     /locus_tag="MB219_002619"
     CDS             complement(978..1223)
                     /locus_tag="MB219_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428284.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1293..1547)
                     /locus_tag="MB219_002620"
     CDS             complement(1293..1547)
                     /locus_tag="MB219_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428285.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT          611 a          302 c          312 g          483 t
ORIGIN      
        1 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
       61 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
      121 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
      181 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
      241 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
      301 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
      361 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
      421 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
      481 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
      541 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
      601 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
      661 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
      721 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
      781 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
      841 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
      901 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
      961 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
     1021 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
     1081 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
     1141 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
     1201 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
     1261 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
     1321 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
     1381 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
     1441 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
     1501 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
     1561 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
     1621 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
     1681 gcaggaactg ttccgctgaa tgtgtctt
//
LOCUS       DAHEYX010000282         1705 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHEYX010000282 DAHEYX010000000
VERSION     DAHEYX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.290"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..366)
                     /locus_tag="MB219_002621"
     CDS             complement(<1..366)
                     /locus_tag="MB219_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8428286.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSI"
     gene            complement(500..1690)
                     /locus_tag="MB219_002622"
     CDS             complement(500..1690)
                     /locus_tag="MB219_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCC8428287.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          487 a          355 c          314 g          549 t
ORIGIN      
        1 aatactacct acacctttat ctgcatttga aatcaattct tctaagattg tttgactttc
       61 agaatttaaa gaaatggaag cacaaatctc attgtcaaat tcttcttcgc tgattccttc
      121 aaatgtcgca gagataataa tccaatgccc ttgccaccca ttttgaattc catgaaaaaa
      181 atctgattct tttagtgttt tactactata gtaataacta tcatctaata agattctcaa
      241 tgcagtaaga gcattggttt tccctgaatc attctcacct ataatagtat ttgtctcatt
      301 atcgaatcga aagatttcgg attcataatt tctaaaattt cgaatttgaa gagtagatat
      361 aaacataagc agatctccta actatttaat taatatgatt atatagtaag tttatcataa
      421 attatgttag taaaagttct cattataagg gagcgtcaat aattttgtgt aaatgattct
      481 tccctactgc agattgtttc taattctcaa tcagttggct tagcatttgt tcgagctcgc
      541 cttcggcctg ttggaaacct ttatgactgc gacctaggaa tttttggttg taagtatcaa
      601 aagacgagac taagaaacgt tccatcgact cttcattttg gaactgttcc ttgcggtggc
      661 tgtatttctt gatttgttta ttaaaggatt caatcaagtt cgtagaataa atactcttgc
      721 gtatggccaa ggggaaatca tagaaagtga gcaagtgatc gttcgaaaga atcgattcaa
      781 ccacttttgg atagctggtt ttccattttt ccgcaaaagt accacgtgct tccaacgcca
      841 cctctttaga tgaggcttga tacaccattt taaaatcatc acagacttcc ttacgatcat
      901 cgacacgcac tttatgactg atattacggg aaacgtgtac acaacaatgt tgaaaacgag
      961 ctttgggata gaaccgacta atcgctccta ccatcccctt taagccatcc gtgatgaaca
     1021 ggaggacatt tttcaaaccg cgctcttgaa ggtccaataa aatttcctcc caaatcgtga
     1081 tggattcagt cggtgcaatc gcatagctca atacttcctt tgatccgtcc gggcgaatgc
     1141 caactgcgat atgaatagct tccttggcga cggttttccg ctttaacgga atatacgttg
     1201 cgtccatata aatagcagca taacggtcat gaagctcccg cccattaaac gccgttacct
     1261 cttctgtaaa tgattttgtt atattggaca tggtttgggg cgtgtagtaa tgcccataca
     1321 ttttctcaat caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga
     1381 cggtctcctc taacgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact
     1441 caccattgcg gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac
     1501 ggtcatagga gccgttacga gaattacccg tgttaaaacc aatgcgatca tacttttcgt
     1561 aatctaggaa agccgttaac tccgttttga gaagcgtatt gactgctttc tccaaatgga
     1621 cacggaataa ttcattcaaa tcgcctttgt tcgctagagt ctttagaatt tctgtagtaa
     1681 aatcgttcat agggaagtcc tctct
//
LOCUS       DAHEYX010000283         1681 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHEYX010000283 DAHEYX010000000
VERSION     DAHEYX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.291"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            49..423
                     /locus_tag="MB219_002623"
     CDS             49..423
                     /locus_tag="MB219_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCC8428288.1"
                     /translation="MENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYKKSLIE
                     NNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLLPLVGI
                     ITTGICLFKKGKDNKTDSSQVS"
     gene            922..1581
                     /locus_tag="MB219_002624"
     CDS             922..1581
                     /locus_tag="MB219_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HCC8428289.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
BASE COUNT          601 a          187 c          303 g          590 t
ORIGIN      
        1 gtaaataaat acataaagta gcattagtag gttttggggg atatagattt ggaaaatatt
       61 tttgatttaa acaagggatt atcaagaaga aaatattggt attatcagtg tatactgttt
      121 gtgtttggat tagggataga aactttttca atgtttgtat acaaaaagtc tctgattgaa
      181 aacaattatt taaaatattt cgtgataaat ggtccatttg atttttataa ttttcttagg
      241 atcgtgttct ttataatgat tattataggt tttctgttcc aagcaagaag gtttgttgat
      301 attgggtggt cgaaatttgt agctttacta agtctgttac cgttagttgg tattatcaca
      361 acagggattt gtttatttaa aaagggaaaa gacaataaaa cagatagcag tcaagtttct
      421 tgattgctat ctgttttatt tattaagagt attgtctatt aaatgaatga caagaacttg
      481 cattgtaagc agcttgaatc gcagctgacc atgagagttt atggaaggct actgcagacc
      541 aaattccttt cataatacaa gcaccttgtt ttgctttagc ttgttgatat ttattccata
      601 aatgtttagg gactttactt tttgcaaagt tgtcctaata accatcgtca ctttgggcat
      661 atgcagtagt aaagtttact aacggataat acatgataaa tttaaaaggt agggtacttt
      721 ttcgtacaag aatgattttg taagtatgaa acaagctagt tatatttcac tagctattca
      781 atgaaataag tttttaaagt agattgttga tagatatatt aaaatttttt ttcatgtaag
      841 agtatgctaa aattgatgtt aagaatggtt ttccctagta ataggtaaca tcaaaataaa
      901 ggaaggtgta attctatacc tatggaagaa aataatatag aaagcaaaga caatctgttt
      961 aaagagattg gggaaaaaat gtatccgctt ttttttaatc gtaattttgg attagcaaca
     1021 aaaagtgaaa ttgaaagcat gctcttttac tcgtatctaa aacataagat aaatagtaat
     1081 agtgattact cagatagaac cttgtctaga gagctaggaa tatcggaagt taaagtaagg
     1141 aatcttaaga gaacatgtta tgcaagatat gaagatgcaa ttgattttcc taaattacta
     1201 acctcgctgt ccaaagaagg ctcaaatcta atgtttttta aaatctatac tgacaataat
     1261 gatgtattat gtaggatatc tatttccgag gtagtttatt atgatgaact tcgaacacaa
     1321 ttaaacaata gtggtattct ttttaaaaaa aatccaggat caaattatgt agagttatca
     1381 atggcagatg caataatttt ctttgatcag gattctatca gcaaagaaag cttagatttg
     1441 aaaaatttaa tagataagat gaaacagttg aattttaaaa ctaatactaa aatggatatt
     1501 gataaaatat tagaaggaac taaagctggt agaatgaaaa atgtcgttga ggctatgatg
     1561 aagctttcca actcaatata aacaaagagg tcagaaactt cctaagtttt ttgacctatt
     1621 gctttttcgt gtgtaacttt tttatttaaa tggcataagt ttttatcgat gaatggcaaa
     1681 a
//
LOCUS       DAHEYX010000284         1665 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHEYX010000284 DAHEYX010000000
VERSION     DAHEYX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.292"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            114..1034
                     /locus_tag="MB219_002625"
     CDS             114..1034
                     /locus_tag="MB219_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCC8428290.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            1188..1532
                     /locus_tag="MB219_002626"
     CDS             1188..1532
                     /locus_tag="MB219_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCC8428291.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          567 a          261 c          331 g          506 t
ORIGIN      
        1 ctgtttgcta aaattcttct catttgagtt cgaagtattt tattttaata gataaactgt
       61 tattcttaca tttggaatac caaagtaggg gagaggtgct tgtgagtaaa attatgactg
      121 cgtatcataa aaatgaaaga ttaaagaaaa tagtggttat tttgataacc ggattgaccg
      181 ctgcggtcgc tttaaattat tttttgattc cagccaatgt cttttctgca gggatgaatg
      241 ggatcgctca aatcatttcc tcgcttttat atgaatggtt tcatatcaag atagacacag
      301 gaacatttat ttttcttttg aatatccctg tgtttgtatt agggtttctc aaagtaggaa
      361 aacgagcaac gatccttagt tttgtgaatg ttgtatgtgt atcggtgatg acgatgctgc
      421 ttccagtcgg acagatcact gataatattt tgatgaatgc cctagtaggc ggtgttttat
      481 taggtcttgg cgtaggatta tctttaaaaa tgggctttac aactggtggg atggatatca
      541 tctctcttgt actttcacaa acaactggaa aaacagtcgg aaactacatg ctgattctaa
      601 atggtatcat tgtagtcatc gcaggcttct tctttaactg ggaaagtgcc ttgtatacga
      661 ttatttccat ttatgcaatg actcaagtaa ttgatgccat ccacacgagt catcaaaaag
      721 taacagcaat gatcgttacc gtcaagccag aagaggtaac agaagaacta gcaaaacgga
      781 tggtacgtgg aatgacatta ctgccgtctg tcggtggcta ttcaaaaaaa caagggaaca
      841 tgatcatgat ggtcatcacc cgctacgaaa tgtatgatct tgatcagatc gttcatgaaa
      901 tcgacgacga tgcatttatc aatattttac caactcattc agtatttggc cggtttgcaa
      961 atgaaaacga acaaaaaatg tatcgaacaa ctggcgtatt gccagaaata aaaaatcgtt
     1021 cacaaaaaaa atgaggaatc aggtttattt tagaataaat ttaatctatt acatctaata
     1081 agaacatttt tataaattta aacgagaatc ttacttctaa attctcattt acaagcagct
     1141 aaaaaaagag tatgataaat aaaaatcgtg ttaaggagtg tcgtacgatg agcgaagcaa
     1201 accaagaacg gtctggacaa gaaatagaac taatcaaaga acgtattctt gctgccttgg
     1261 aaatggtgat tgatcctgaa ttaggaatcg acatcgtcaa tttaggcttg atttatgata
     1321 tagaattcaa tcctgaaaat ggagaaacag tcattaagat gactctaaca actatgggct
     1381 gtccattagc agatatttta acagaatcta tccatgacgc attaaaagaa gtgccagaag
     1441 taacgaaagc agaagtcaaa ctagtctggt atccagcatg gacgacagat aagatgagcc
     1501 gttatgcacg gattgcgttg gggatacgat agaaatcttg gctgactagc tatgagaagc
     1561 aaataggaaa aacaaaaaag ccttttatcc tttctagaag aaaagagata aaaggtttct
     1621 ttgtttataa aattttgtct gctatattat aagttaatta aataa
//
LOCUS       DAHEYX010000285         1633 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHEYX010000285 DAHEYX010000000
VERSION     DAHEYX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.293"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            612..1424
                     /locus_tag="MB219_002627"
     CDS             612..1424
                     /locus_tag="MB219_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428292.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          546 a          238 c          273 g          576 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tat
//
LOCUS       DAHEYX010000286         1611 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHEYX010000286 DAHEYX010000000
VERSION     DAHEYX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.294"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            84..1562
                     /locus_tag="MB219_002628"
     CDS             84..1562
                     /locus_tag="MB219_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428293.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          577 a          222 c          295 g          517 t
ORIGIN      
        1 tcataaaatc agtataggtg aaaagatgaa ctttcatctg catatcaagt aattggtatg
       61 aggcattttt tgaggtgtag gatatgaagt tggattacct tatagaagag gaagatagag
      121 aaaaactgct gctagctaga tatttagaat ttgagaaaaa gaattatgta gtgctgccta
      181 agttatatga aacattaggt ttgtctaaat ttaaaacaag aaattatatc atagaattaa
      241 ataaagaatt tactgaattc aaaacaaagc cagagatcca aatttcagaa gaagcagaga
      301 tccatgtcga acgtctgaat ttgacggata tcaaaaattt aagaagaatc tactttcaac
      361 gatcaaaaat tgctcgactg ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat
      421 ttgcagagaa tcatttttta agcatatcaa gagcctatgt aaaaagaaga gaattagtct
      481 cttttcttga acagcatcaa ataaagttaa agaaaaacat attagttggg gaagaaataa
      541 acatacggaa cgtattattt ggggtatatt tcggggtctt taatggtttt tctatgccat
      601 ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa tatctgttca
      661 atttaaaact gtctgcaaca aaacgaatca aattcaattt gctaaccact attagtttgt
      721 tgagggccta tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa tactttaaga
      781 gctcagaagg aatgaagatc atcaaggatt tatcacatat ttcttttctt catatcccta
      841 aagagcagat cattgatgag atttcttata tcatgttgtt tttgatcacg gaagaagaat
      901 ttgaattaga tggcgcattt ggacttgcgg aagctcattt tcatgatatc cacatgatta
      961 gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt ttcgattgaa atctctgaag
     1021 aagaaagact atatatacga aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa
     1081 attggatatt cgactttgag atgactagct ttacgtctaa aaagcagcta cagtttttct
     1141 tcgagacgta tccaatattc agtacagcaa tccaaaaggt catacaagag aataaacgct
     1201 atttcttggg aaaagatgaa cgtttcatga ttcgcttatt ttatgattat ctctttttat
     1261 tggtcggtat ttgtccggta tcatattttg aagcaccgat tcatgtctgt attgattttt
     1321 cgcaaggatc tgcttatacc gattacatta tttctcaagt gaaaggattt aaaaattaca
     1381 atattgtgat tgagagtcga atcacttctc gtacgaatat ttatttatct gattgtttac
     1441 ttgaaaaatt taataaagaa cagataattt ggaaaaatcc accgactcca gatgactggg
     1501 aatattttgg aaataccatc atcagagaaa aaaatagaca tttaactgaa aaaatcagct
     1561 agtatttatg gatggcagct ttgtctcaca tatttattag ctgatttatg t
//
LOCUS       DAHEYX010000287         1594 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHEYX010000287 DAHEYX010000000
VERSION     DAHEYX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.295"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            122..952
                     /locus_tag="MB219_002629"
     CDS             122..952
                     /locus_tag="MB219_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCC8428294.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            924..>1594
                     /locus_tag="MB219_002630"
     CDS             924..>1594
                     /locus_tag="MB219_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCC8428295.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSF"
BASE COUNT          577 a          201 c          330 g          486 t
ORIGIN      
        1 atgtcctcac aaggactaga tgaagatgaa aatatcagtt cattagcaga aagtgcattt
       61 ccaaaagaaa aaacgtatcg ttagattgga aaaattttga gcaaagaagt aggagagaac
      121 attggaaaat aaaaatataa gtatactgct ttctttgtat gataaagaaa agccggaata
      181 tttaaaggaa gcattagcta gtatctttga gcaaacggtg atgccggcag aaatcgtatt
      241 ggtttatgat ggccctataa atgagaagct tcaggcagtt gtcacttatt tccagcaaaa
      301 agttcctcac ttgtttacag ttgtaaaatt agaaaaaaat caaggattgg ggattgcttt
      361 agcaactgga ttagaacata ctaaaaatcg cttagtggcc agaatggata cagatgacat
      421 catggaacca cacagattgg aaaaacaatt agagatcttc caagaatatc cagaaatagc
      481 gattgttggg agtaatattg aagaatttac tggcacactt agcaatgtta tcggaagaag
      541 gattgtacct gagtataatg atgaaatccg tgtgttttcg aaaagaagaa acccttttaa
      601 tcatatgaca gtcatgtttg ataaaaatgc tattttagaa gtgggaaact atcgcccatt
      661 aattggattt gaagattatt atttgtgggt aagactattg aaggcgggat ataaaggata
      721 taatatccaa gagattctag tacatgctag agcaggtgct gacatgtatg ctagacgtgg
      781 aggaagtaaa tatcttttac caggaattaa aggccgatat cgcgtatgga aagatggtct
      841 aggatcacta aaagattttc tgatagttat ttgcggacat cttgtaatta gtttattacc
      901 gaataaaatt aggggaaaag tatatgaatc aaaacttaga aaaacaaact aaactttcta
      961 taatcatacc tttttacaat gcaaagaata ctttaagaag agctgtagac agcgtcttaa
     1021 cgcaaggtta tcccaatatt gaaatcttgt taattgatga tggatctaca gatggtagta
     1081 gtgacattgg tgacgaaatt gctaatctag agagtaaagt agcatgttat cacttggaaa
     1141 acgctggtcc atcagttgct aggaactatg ggataaaaga agcaactgga aatttggtag
     1201 gatttttaga tagtgacgat tattttttac ctgattgttt taataatgtt ttgaaagact
     1261 accataggaa tcaccccgat atcattgctt ttggattaag taaaggaaaa aatatagaaa
     1321 atgcaaattt atttgttccc gaaaagaaga ttggttgttc aagtgaagaa gcaattgagc
     1381 aaatgtttag aagtaaggca gtggactttt acgtatggaa taaatttttt aaaagggaat
     1441 tattcaatca tatagttttt ccagaaaata aattatatga ggatatggtt cctatgtatg
     1501 aagcttttag ggcggctagt aaaattgata ttttgccatt ttcaggtatt ttttattatc
     1561 aaaataacga tagcatcgtt tatcagtctt ttaa
//
LOCUS       DAHEYX010000288         1547 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHEYX010000288 DAHEYX010000000
VERSION     DAHEYX010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.296"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            25..303
                     /locus_tag="MB219_002631"
     CDS             25..303
                     /locus_tag="MB219_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428296.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            322..1530
                     /locus_tag="MB219_002632"
     CDS             322..1530
                     /locus_tag="MB219_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCC8428297.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
BASE COUNT          458 a          257 c          293 g          539 t
ORIGIN      
        1 tttttaagaa tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta
       61 gaaacagcat atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg
      121 tatcaaaact cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa
      181 gaatttttaa atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc
      241 tatccatcca attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct
      301 taaggggaat gtataacata catggaaaag aaattattta ataaatattt tattggaatc
      361 acattgatca attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc
      421 attgcgcaag acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat
      481 atcatcggaa cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg
      541 aagtttacct taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat
      601 acgccaacta tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca
      661 gtttctacag ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa
      721 ggcatcaatt attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga
      781 atgattttgc tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc
      841 ttctttgtta cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa
      901 caaaaaatat ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc
      961 attacaggaa ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct
     1021 tcttatacaa aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct
     1081 ttggtcatca catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa
     1141 tacgtgatgt atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg
     1201 acgaatagtg tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt
     1261 atgtcaaatg gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct
     1321 ttatctacct actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg
     1381 atgctaaaat ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg
     1441 atatgtacag cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa
     1501 ttgattgaat cattgaaaaa tgaaatttga tcttgatgaa tagataa
//
LOCUS       DAHEYX010000289         1532 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHEYX010000289 DAHEYX010000000
VERSION     DAHEYX010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.297"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1342
                     /locus_tag="MB219_002633"
     rRNA            <1..1342
                     /locus_tag="MB219_002633"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1532
                     /gene="rrf"
                     /locus_tag="MB219_002634"
     rRNA            1437..>1532
                     /gene="rrf"
                     /locus_tag="MB219_002634"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          450 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tg
//
LOCUS       DAHEYX010000290         1520 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHEYX010000290 DAHEYX010000000
VERSION     DAHEYX010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.298"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            9..1475
                     /locus_tag="MB219_002635"
     CDS             9..1475
                     /locus_tag="MB219_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCC8428298.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          321 g          493 t
ORIGIN      
        1 tatagattat ggaaaaacag caagacaagg gcttcttcgg gcagccgaag ggattgtcaa
       61 ctttgttctt cactgagatg tgggaacgat ttagttatta cggtatgcgt gcattactta
      121 tctattatat gtacgataca gttgccaacg ggggacttgg attaccacga gcaacagcat
      181 tagctatcat gtcgatctac ggatctttga tctacatgtc tagtatcgtc ggcggatggg
      241 tatctgaccg agtgttagga gcaaaacgca ctgtattctt cggtggagtt ttcattatgc
      301 ttggccacat cgtcttggct ttaccatttg gtgtaagtac attgtttgtt tcgatggcat
      361 tgattattat cggaaccgcc tttttgaaac caaatgtttc tggcatggtc ggacatcttt
      421 attcaaagac tgacttgcga agagatgcag gattttcgat tttttacatg ggtgtcaact
      481 taggggcatt attagcacca ctaatcgttg gtacaatcgg tcaagaatat aactatcatt
      541 tagggttctc tttagctgct attgggatgt tctttggatt attacaatac atcatccaag
      601 gacgcaagac attagacgga gtgggtacaa caccgccgaa tcctttgaca actgaagaac
      661 gtaaaggatt catccgtaac ttgatcatcg cacttgttgt tatcgctgca gtattcggcg
      721 gggcacaact aactggtcat ctgacaattg acttttttgt caatacaatc agtgtattgg
      781 ggattatctt accgttgtat tactttatca aaatgctgac ttcaaaagat gtaactgctg
      841 aagaaaaacc aagagtatgg gcttatatcc cattattcct tgcagcaatc attttctggt
      901 cattacaaga acaaggttca tctatcttag ctttatttgc tagtgaacgt acacaatcta
      961 cgctatttgg tgtaacgatc caagcaagct ggtatcaatc attgaatcca attttcatct
     1021 ttattctgac accagtattc gtaacgatgt ggacgaaact tggtgaacgt caaccatcta
     1081 cagtagtgaa attctctctt ggacttttat ttgcaggatt atcgtacttg ctattgatgt
     1141 tgccgggtat gctttatggt acaagtggaa aagtaagtcc attgtggttg ttgggaagtt
     1201 tcttcatcgt ggttattgct gaattgtgtc tttcaccagt tggtttgtct gtgacaacaa
     1261 aattagcacc acgagcattt gaatcacaaa caatcgctat ttggttctta gctgatgctt
     1321 cttcacaagc aatcaatgca caaattgcac gcttctacac accgggaaca gaatctgctt
     1381 acttcggtat tatcggtgca gtagcaatcg tcggtggtat cttgctattt actgtgaaag
     1441 aaccaatcaa aaaactaatg ggctctattc attaatcaat gaacataaaa acaggaattg
     1501 cttatttgca gcagttcctg
//
LOCUS       DAHEYX010000291         1520 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHEYX010000291 DAHEYX010000000
VERSION     DAHEYX010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.299"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            124..1476
                     /locus_tag="MB219_002636"
     CDS             124..1476
                     /locus_tag="MB219_002636"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCC8428299.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          537 a          270 c          312 g          401 t
ORIGIN      
        1 ttgaaaagga atgaaactac aaacaaacct ttgtgaattc acaagcatta aactgttgta
       61 tccgttttat ttctgttata gtagttttgt cgaaggacaa aatgttaatc ggagggacca
      121 taaatgaaag tagtagtaat tggctgtact catgcaggta ctgcagctgt aaaaagtatt
      181 ttaaaaaatc atccagaagc agaagtgaca gtatatgaaa gaaatgacaa tatttctttc
      241 ttatcttgcg gtatcgcact ttatgtcggc ggagtagtaa aggacccagc aggcttgttc
      301 tactcaaatc ctgaagaatt atcttcatta ggagcaacag ttaaaatgga acacgatgta
      361 acagacgtcg atacagaaaa taaaacagta acggcaaaag atctgaaaac aggagaaaca
      421 gaaacggttt cttatgataa attagtgatg acaactggtt catggcctat tatcccaccg
      481 attagtggaa tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca agcaaatgaa
      541 attattgcac aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg atatatcggt
      601 attgaattag tagaagcttt tgtcgaatct ggaaaacaag tgacattgat tgatggatta
      661 gatcgcatct tgaataaata tctagataaa ccattcacag atatcttaga aaaagaattg
      721 acagaccgcg gtgttactct tgcgctaggc gaaaatgtta ctgaatttat cacagatgaa
      781 gaaggaaaag tgaaacaagt agctacacca acagatacat ttgatacaga tatggtcatt
      841 ttatgtgtgg gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga tacactgcca
      901 aacggggcaa tcaaagtgaa tgaatacatg cagacaagta atccagacat ttttgcagct
      961 ggagactctg cagtagtcaa ctacaatcca agtggcacgc aaaactatat tcctttggca
     1021 acaaatgccg ttcgtcaagg tttgcttgtg ggaaataact tgacagatca taaaatggcg
     1081 tatcgtggta ctcaaggaac ttcaggactt tacctatttg gctggacgat cggttcgaca
     1141 ggtgtgacca aagaaagcgc aactttgaac gatctagaag tccaagcgac tgtatttgaa
     1201 gataattatc gtccagagtt catgccaact actgaaaaag taatgatgga gctagtctat
     1261 gaaaaaggaa cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga cattacacaa
     1321 tcagcaaata ccatgtcttt agctgttcaa aataaaatga ctgtagaaga tctcgcactt
     1381 tcagatttct tcttccaacc acattttgac cgcccatgga attacttgaa cctacttgca
     1441 caagcagcgc taggcgaaat cgaaactgca caataaagaa aaagggttgt gacacaattt
     1501 tgtcacgacc ctctgtcttt
//
LOCUS       DAHEYX010000292         1515 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHEYX010000292 DAHEYX010000000
VERSION     DAHEYX010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.300"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            36..632
                     /locus_tag="MB219_002637"
     CDS             36..632
                     /locus_tag="MB219_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCC8428300.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(690..1172)
                     /locus_tag="MB219_002638"
     CDS             complement(690..1172)
                     /locus_tag="MB219_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428301.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1278..1475)
                     /locus_tag="MB219_002639"
     CDS             complement(1278..1475)
                     /locus_tag="MB219_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428302.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          513 a          319 c          241 g          442 t
ORIGIN      
        1 aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga
       61 aaaaccgccg ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa
      121 caactttgat caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac
      181 gcgcagttat ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac
      241 ttcgcccatt gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca
      301 aaaatggtta tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag
      361 aacaatttga attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg
      421 caacagctaa tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt
      481 acaacccagt tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac
      541 tacgttcgca attagtattc ggttcaccag aaacaccggc tggggaaaaa gaatacatga
      601 acgatgccga tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg
      661 tgacaaacat cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct
      721 gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat
      781 ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct
      841 tttggctgat ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag
      901 ctattcaaaa caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa
      961 atcattgctg acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta
     1021 taccaaatga ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca
     1081 gtccgataaa taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat
     1141 aaaaacaata gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca
     1201 gaaaatagga aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc
     1261 acgtccaatt gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt
     1321 tcatctaata attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt
     1381 gtcatttttg atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca
     1441 tattgttctc ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac
     1501 ttttctccac attac
//
LOCUS       DAHEYX010000293         1501 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHEYX010000293 DAHEYX010000000
VERSION     DAHEYX010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.301"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            17..901
                     /locus_tag="MB219_002640"
     CDS             17..901
                     /locus_tag="MB219_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCC8428303.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            1015..1251
                     /locus_tag="MB219_002641"
     CDS             1015..1251
                     /locus_tag="MB219_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCC8428304.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            1339..1419
                     /locus_tag="MB219_002642"
     tRNA            1339..1419
                     /locus_tag="MB219_002642"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1373..1375,aa:Tyr,seq:gta)
     gene            1426..1497
                     /locus_tag="MB219_002643"
     tRNA            1426..1497
                     /locus_tag="MB219_002643"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1458..1460,aa:Gln,seq:ttg)
BASE COUNT          509 a          206 c          367 g          419 t
ORIGIN      
        1 aaaggagaat atttttatgg gaatattagt ttttgatttt ggtggatcgg ctgtcaaata
       61 tggttgttgg gatggcaaag agattaaagg taagggacaa tttgcaacgc ctgaatcttg
      121 ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa atactcgttt cttttgaagt
      181 cgaaggaatt ggaatcagct ctcctggagt agtgaataat gagaaacagg tgattgaggg
      241 aatcagtgcg attccttata ttcatggatt taatattttc cgagatttgg aaactctgtt
      301 tcaactccca gtaacgattg aaaatgacgc caactgtgcc ggaatggctg aattttatga
      361 aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg gcaggaactg ggattggcgg
      421 ggcgttgttc acgaaaggac aaataaataa aggggcacat ctttacggtg gagaatttgg
      481 gttgatgttc ttagatggcg ataagacatt cagtaaactt ggtacagcgg tacagatggc
      541 ttggcgttat tgtgaacgca aaggtctcga taaaaatgct tataccggaa aagatgtctt
      601 tgaattagca gaaactggag atgcaatagc aaaagaagaa gttgagagtt tttatacgta
      661 tcttacaaag ggattattca gtattcaatt ttcgtttgat ccagaagtga tcgtattagg
      721 cggcggggtt tctgcaaaag aagggctcat tgatgagatc aaaagccgaa tgaagaagct
      781 gacagaacaa ttcgatctgc atgatttcga accgcagatt ttattatgtg agtacagaaa
      841 cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag gagagaatta
      901 agagctttag agaaggtgca gcttgcataa aagaagagaa tgaaatgaat aggagatttt
      961 tacctaagga atgttttatt tgctgcagta tgataaaata tctgagaggt gaaaatgatg
     1021 tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt
     1081 aagaattatg aagtcactca gttatttata gggaaaaaaa tgattggtga gatcttagcc
     1141 tatgggccaa aagaatatga aattttcttt ggtgaagaag attttggaaa agaaaaaagt
     1201 cttgaaaatg cgatagaaac agtgattcga cactggaatc tgcacgaata aaaaaatatc
     1261 aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat taggtattat aactaagtca
     1321 ggttgataca aacctttcgg aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc
     1381 gttccttagg gttcagtggt tcgaatccac tctcctccat ttcattgggg tatagccaag
     1441 cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct accccagtat
     1501 a
//
LOCUS       DAHEYX010000294         1490 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHEYX010000294 DAHEYX010000000
VERSION     DAHEYX010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.302"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            115..666
                     /gene="lepB"
                     /locus_tag="MB219_002644"
     CDS             115..666
                     /gene="lepB"
                     /locus_tag="MB219_002644"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCC8428305.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(749..1249)
                     /locus_tag="MB219_002645"
     CDS             complement(749..1249)
                     /locus_tag="MB219_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCC8428306.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT          544 a          246 c          272 g          428 t
ORIGIN      
        1 gtccaatagc ctaattgaaa gaattatttt tatttcagct atctagtcgc atttttttga
       61 tatttttctt ataataaaga aagaatgaaa aaaagaggga tggattgata gagattgaca
      121 aaaaaacaac gctatattaa tcgcttctgg cttgtattca aatacttact tgtttctgta
      181 ttccttgcgt tcatgttgcg gggattttta ttcatcccgg taccagtgga aggcaattca
      241 atggaaaacg tgttgaagca aggtgatatg gttgtgatgg aaaaattttc ggagatcaga
      301 cgatttgata ttgttgtttt tcaattagca gatggcacga tttatatcaa acgagtaatc
      361 ggtcttccag gagaaaatgt cagctaccaa aatgatcaat tgaagatcaa tgggaaagta
      421 gttaaggaac cttatctaac taaaaacctg aaatcagatc atgcaaatgc atcctatacg
      481 acggacttta cactacaaga gctaacggga caatccaagc ttccggaaga taattatttt
      541 gtacttggag ataatcgaag agtttcaaaa gatagtcgtt cgtttggcac gatcaataaa
      601 actgatattc taggtaaagc aagatttgtc tattatccac ttgatgaaat aaaatggatc
      661 cagtgagaaa caataaagcg tgcagtctgt tgagactgca cgctttattg tttaatccaa
      721 aatcagttta ctaccttagt acgtaaaatc aaacagctcg tttaaacact tttctagcct
      781 ctaaagtttt gataaatgaa taaacaacca ttatttgaat agggatttgg atcatattcc
      841 tgatgaatct acctagaaaa attactttga aatctgcacc aaacaaaaca gacatcagcc
      901 aagaattgat aaaaaggctg actgggatca taatcaataa ttgggcagcg atgacggaag
      961 gcaggtttat ctttttatga tataaaaata agccattaat aaatccacca aggaaagccg
     1021 tgataagaaa agcagcttga aacgttcctg aaccaaatat cgtgaaattt aaagtgttcg
     1081 acaacatttc aagcaaacca gcccagatcg gtccatatag ataacccata agcgccatac
     1141 ttaaaaatcc gaatgtgatt tgaaaataag caggaccaat cgctatcttg tataagatga
     1201 catcaagggc taataaaaga gcagaggtcg acaacaatct tgcattcatt tcgggcaatc
     1261 taaaaacgtc ttttgcaatc aaagtgttca aaatattctc tcctttgtca gttaataata
     1321 aaaggatacc ataaaatcac caatgtgtca tgacacattg gtgatttaca tacactggca
     1381 tctaacaatt aaaaaaaggg tgagaagact gcttttataa actgtaaaaa agagattgta
     1441 cgaataaaaa aaatagaggc ggagaatact tatcgtatca tccgcctttg
//
LOCUS       DAHEYX010000295         1486 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHEYX010000295 DAHEYX010000000
VERSION     DAHEYX010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.303"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            65..505
                     /locus_tag="MB219_002646"
     CDS             65..505
                     /locus_tag="MB219_002646"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCC8428307.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            633..1316
                     /locus_tag="MB219_002647"
     CDS             633..1316
                     /locus_tag="MB219_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCC8428308.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT          497 a          240 c          308 g          441 t
ORIGIN      
        1 aaaaataact tttataagtt ctataacgtt gattatattt ttaattctta ttcttctcgt
       61 cgtcttggtt ccccgaattt ttggtttgac tccttatatt attgaggaca aagaaatgag
      121 tccatattat gcacaaggaa atctgatata tgtacgaccg aaaaatacaa atgagatttt
      181 agtcggagat attattacat attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt
      241 aatagccgtt gaaaacaacc acgaagattt ttatacaaaa gctgatacaa agacttatat
      301 tgagcctgga gtagtaaaaa agagaaatat tatcggtagt cccatctttc agattccata
      361 tattggacta gtcctttcaa aaacggcatt tgcatggatt caggggttat tctttacgat
      421 tgctttttgc ctaacaggaa tcacagcatg gaataccttt gtagagttga aaaagaagcg
      481 gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc cagagatcca
      541 acgactcttc tgaacatgaa acacgagaaa acactcatgc agcttgtaga tttagtcagt
      601 tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg
      661 tagtcaaaga agaactattg gattttatgc gtacccagca aaaaaaatta ccgggtgagc
      721 taggcaaact ggaagaagaa gctcatgtag cagaagtacc agttattccc catgaaacag
      781 ttgtattttt gaaattttta cttggacagc ttcagcctga acgaatcttg gagatcggag
      841 cagctatcgg tttttcatca agtgtcatgg ccacgacaat cggagaaaac gctcatgtta
      901 cgacaattga tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat gaacgtctag
      961 gactgacaga taaagtgact ttgcttgaag gtcaagcagc agatatcttg ccggagttgt
     1021 ctggtccgta tgactttatt tttatggata gtgcaaaatc aaaatatatt gaatttttac
     1081 ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat ggtagatgac atcttccaag
     1141 gaggaacgat cttgctccca gatgaagaga ttccccgtgg caaacgagct atccatcgta
     1201 aattaaatga atttcttcgt gttgtgatgg atcatcccga tttgacatcg acaatcttgc
     1261 cattaggtga tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag ttgtgataca
     1321 tgattatggc agcctgtaga taatcgattg aatcgagggc tcaattcgaa cttgatgtaa
     1381 aagtttctat atttgtaagt caattaaagg atacaggaaa tctaaataaa caggagtagc
     1441 atgaagtcaa tgtagctcct gttttcaatt gataacctta gaacag
//
LOCUS       DAHEYX010000296         1485 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHEYX010000296 DAHEYX010000000
VERSION     DAHEYX010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.304"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..360
                     /locus_tag="MB219_002648"
     CDS             <1..360
                     /locus_tag="MB219_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCC8428309.1"
                     /translation="ETKSLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSE
                     RMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKII
                     QLLEMPIFLMPANEKNN"
     gene            397..1407
                     /locus_tag="MB219_002649"
     CDS             397..1407
                     /locus_tag="MB219_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCC8428310.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          525 a          241 c          323 g          396 t
ORIGIN      
        1 gagaccaaaa gcttattctt aaaagataaa aaagataata actacgtata tctgactttt
       61 actacgaaac aaacggattt caaagcatta gggaagatcg tcggtaaaag gctttctatt
      121 gtttcatcag aacgcatgga agaacaaacg ggacaaaagc agggcgctgt ttctccgttt
      181 ggttatgaaa aacagattcc gattattatt gatgaagagc tcttatcaaa tgaaaaactt
      241 gtttttgcac caggtagacc agatcaaacg atggtcatcc aaacggttga cttaaacaaa
      301 atcatccagc tacttgagat gccgatattt ttgatgccgg caaacgaaaa aaacaattaa
      361 ccaagtaaaa tagtgcatac ttggaggtga agcaacatga taagttatcc attattagaa
      421 agaaatgcaa cgattggggt tactgcacca tcctcaggcg tatcagagga acaccattcg
      481 ttactcgaat cagctttaaa gagaatggaa gaaaaatctt tccaagtagt tactggaaag
      541 acgctttgga cgcaagataa ggaaaaatca ggtccagcaa gaaaacgagc agatgaattc
      601 aatcaaatga tgcaagatga aaatatccaa atgatctttc cgccttgggg aggagaatta
      661 ttgattgaga ttctcgattt tgtcgatttt gatgctctac aaccaaaatg gatagtaggt
      721 tattcagata ttagtacgtt gctacttgct gtcacattaa aaacagggat agccactgca
      781 catggaacca accttattga tttaagagga gagtacttgg atgaaacgac agcaatgtgg
      841 gaaagtgtac tgaaaacaag agaaggagaa tcgattctac agcaaccttc tgaaaagtat
      901 caaaaagagt ggccccatga gcattcatca tcttctgttt tccatctcac tgaaccaaca
      961 aattggcaat cagtcagcgg agatgcagta aggatgaagg gacgattatt aggtggctgt
     1021 attgatacga tcagtcattt aatcggtacc ccttatggag atgtccaatc ttttaaaaga
     1081 aagtataatg atggaaagcc gatcttgtgg tttctagaaa attgtgagct atcgtcagtt
     1141 gatttaagaa gatcacttgt tcagatgaaa ctggcaggat ggtttgatgg cagctcgggg
     1201 atcatgttcg gtagaagtgc agcagaggat ttgttagatt ataaaatgaa ggatgtatat
     1261 gaagatctgt caaaggaatt aaaactaccg atcttgtatg atattgattg tggtcatgta
     1321 ccgccacaac tgacattgat caatggtgcc tatgcagaag tagcggtaga agatagaaaa
     1381 gcgactgttt tgcaaacgtt tatttaaaaa attttttcct gccttggagt ttatcaaaga
     1441 ccaacaaatc taaatagaaa agaaaatgaa agaagctgac agtat
//
LOCUS       DAHEYX010000297         1477 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHEYX010000297 DAHEYX010000000
VERSION     DAHEYX010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.305"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(74..958)
                     /locus_tag="MB219_002650"
     CDS             complement(74..958)
                     /locus_tag="MB219_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCC8428311.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1053..1400)
                     /locus_tag="MB219_002651"
     CDS             complement(1053..1400)
                     /locus_tag="MB219_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428312.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
BASE COUNT          463 a          315 c          231 g          468 t
ORIGIN      
        1 aaaagtcttg tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt
       61 cacaacctcc attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag
      121 gtaatttatc cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg
      181 acacaactaa ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt
      241 ttaataggtc tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca
      301 aatggagctt accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg
      361 agttagttag tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt
      421 ctacgtgaaa cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag
      481 ctagttttcc aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag
      541 agccaattgc gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct
      601 tgactgattc aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg
      661 ctcgagccaa gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt
      721 gttcagcgat tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac
      781 tatgataccc ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg
      841 tatgcaagac gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa
      901 attcctttgc ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca
      961 ctttctttcc cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg
     1021 ctgtgatgaa gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat
     1081 actatgaaac tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat
     1141 tgtataccta ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga
     1201 atgtaatagc gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca
     1261 aatgcataac tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat
     1321 gcgacaatcc ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat
     1381 tttatataca aatgatccat tgtacttcct gcctttttat ttgtgagaaa aacaaatccg
     1441 tatacgtgaa taacttctag aaataaaaga aatttgc
//
LOCUS       DAHEYX010000298         1477 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHEYX010000298 DAHEYX010000000
VERSION     DAHEYX010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.306"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1352
                     /locus_tag="MB219_002652"
     CDS             <1..1352
                     /locus_tag="MB219_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCC8428313.1"
                     /translation="DEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLL
                     PLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKK
                     AIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIK
                     AVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYY
                     HHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKK
                     NTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAK
                     SKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGART
                     SGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          492 a          267 c          312 g          406 t
ORIGIN      
        1 gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt ccagtaaaag
       61 gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct ataacagaga
      121 aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca cagatgaaag
      181 atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg ttggattgtc
      241 tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga ctttcttctt
      301 ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc aatagtttag
      361 tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg caagagaact
      421 attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat tggcatatta
      481 aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat cttaatcaag
      541 aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc aaaacagata
      601 cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt tattatcacc
      661 acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt acagggtatt
      721 tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt gtctattgtt
      781 tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa aacacggata
      841 ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa aagaaatgga
      901 aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt ccattattta
      961 ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag ttggatgcag
     1021 ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa gatggtcgtt
     1081 tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata ggacggaaaa
     1141 attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt ctgagtgtat
     1201 ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt ctatttgata
     1261 agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg ccttggaaca
     1321 aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg attctattct
     1381 ataggtcggg ttatttgacg tacaggtact gttctatggg gcgcttacga actttataaa
     1441 attcaattat gacataaaag ccaaacttaa tgatcag
//
LOCUS       DAHEYX010000299         1465 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHEYX010000299 DAHEYX010000000
VERSION     DAHEYX010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.307"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            65..>1465
                     /locus_tag="MB219_002653"
     CDS             65..>1465
                     /locus_tag="MB219_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HCC8428314.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISH"
BASE COUNT          550 a          257 c          240 g          418 t
ORIGIN      
        1 aagctattct gttatagaag aaaaaattgt ttgttattat tagcaacaaa aaaaggagtg
       61 catcatgaaa aagttatctc aaaaacgttt agctcaaatc attattgatt ctagaaaatc
      121 tctgaaaatg acacaacaac aacttgcagt agctactggt atcaaccgtg ccttaattag
      181 tcgagtagaa tctctcgatt ttatcccgtc aatacaacaa ttagaatcat tagcagaagt
      241 tctaaatttt gaaccaacag atctatttat aaatgaatca aataaatcaa acagaacaaa
      301 acatcagtcg aagaatattg ctgtagccgg tactggttac gtaggtcttt ccattgctac
      361 tcttcttgcc cagcacaatc aggtaacagc agtagacatt cttcctgaaa aagtaaaaca
      421 aattaacaat aagaaatctc caatacaaga cgattacatt gaaaaatatc tagctgaaaa
      481 aaatcttaat ttaattgcaa cgactgatgg tgcagccgcc tatagagaag ccgattttgt
      541 tgtcattgct gctccaacaa attatgatag taaacaaaat tattttgata cttctgctgt
      601 agagtcagta atcaaattgg tgttagaagt caatccaaag gcaataatgg tcatcaagtc
      661 gacggtaccc gtaggctata ccgaaagtat tcgtaaaaaa tacaatacta aaaatattct
      721 attcagccca gaatttctca gagaatctaa ggcattatat gacaatctat atccttcgcg
      781 aattattgtc ggcacggacc aaactgacaa aactttacga gaagcctctc acacttttgc
      841 agatttactc caagaaggag caatcaaaaa agatatcgaa actcttttca tgggattcac
      901 agaagctgaa gcagttaaat tattcgctaa tacttacctt gcacttcgag tatcttactt
      961 caatgagctg gatacttatg cagaaatgaa aggactaaat acacaagaaa tcattcacgg
     1021 ggtttgctta gatccaagaa ttggcacaca ttataataat ccatcttttg gatatggagg
     1081 ctattgttta ccgaaagata caaaacaact tctaactaac tataacgata tacctcaaaa
     1141 tatgatgagt gcaattgtcg atagtaaccg aacaagaaaa gactttatag cagaccgcgt
     1201 gcttcaaaaa actggatatt attcttacga tgatgacaat actttcgatt tgtctctaga
     1261 aaaagaagtg attattggag tctataggct tacaatgaaa agtaattcgg ataacttccg
     1321 tcaatcttct attcaaggta ttatgaaaag aatcaaggca aagggagcca ctgtgataat
     1381 ttacgagcca acacttaaag atggtagtac attttttggt tcaaaagtaa taaatgattt
     1441 agaaaaattt aaaagcataa gtcat
//
LOCUS       DAHEYX010000300         1464 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHEYX010000300 DAHEYX010000000
VERSION     DAHEYX010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.308"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            208..1398
                     /locus_tag="MB219_002654"
     CDS             208..1398
                     /locus_tag="MB219_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCC8428315.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
BASE COUNT          560 a          238 c          259 g          407 t
ORIGIN      
        1 tttataagcg aacgtaagag ataatagaca cctaaaaata gaaagaaaaa taaaggtaat
       61 acacgtagta aaacaaatgg agtttcaaaa ataaatggat agcataaaat atcttgtgta
      121 aattcgtttt tctagaaatg tagaaaaaaa ggtatatcct tagtaaaatt agttcgacca
      181 aaaacaattt acgaaaggaa tatacccatg aactatttta ctacatcttt aatgaaaaac
      241 ctattttcta atgacaacaa tcttccagct tttgaacagg ctgtaaccga actgtttcgt
      301 tcagagttgg agaaatctct caatgaaata ctcgcatatg agttgacgga ttttcttgat
      361 tatgaaccat acgctagaac caataacgaa aattctagaa atggttccta tcaaagaaag
      421 tttgatacaa aatatggaac tattacactc aatattccaa gagatagaat gggagaattt
      481 ttcacttctc ttctgcctaa atacagaaga cgagatctat ttaccgaaac aacgattttg
      541 gatttatttg aagaaggaat gagccattca gaaatttgtt ccatgatcca aaaattatgt
      601 ggagctcgct attctaaaca gaccatatct aatattacgg acaaagctct ggaatgtgta
      661 gattccttca aaaacagaat attgaacaaa gagtatgccg ttgtatatct tgatggaaca
      721 tccatgcctt tacgccgtga tactgtatcc agggaaatgg tccatatagc actaggaatc
      781 acagtagaag gtacaaaaga aattcttggc tatatgattg caccaaacga gtccgctgag
      841 atatgggaag aacttctgaa agaccttaaa tcaagagggg ttgaacgtat ttctttattt
      901 tgtacagacg gattgtctgg gatggaaaat gtaatacagg acattttccc gggctctaaa
      961 attcaaagat gcctggtcca tattcaaaga aatatatgtg caaaagtaag agtcagtgac
     1021 agaaaagaag tggccaatga atttaaagag gtatatcggt ccaaatcaaa agaagaagct
     1081 ttaacttctc taaagaaatt catcgatagg tggaaacaca aatatccatc aatggtgaaa
     1141 agtctcagca acaatgacta tctgtttaca ttctatgatt atccagaaag tgttaggcat
     1201 acgatttatt caacaaatct gattgaagga aacaataaac aattaaaacg aagtttcaaa
     1261 aagaaagaac aatttccaac ggaaaaatct gaagagaaat accttgtgat tcagttcaat
     1321 cgttacaacg agaaaaacat gaatcacgta catagaggtt ttggacaaac aaccagagaa
     1381 gattggttca agagctaatc tgctttacca aggatatact tttacacata attcttgacg
     1441 ctccctttta tttagtgcca cttc
//
LOCUS       DAHEYX010000301         1425 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHEYX010000301 DAHEYX010000000
VERSION     DAHEYX010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.309"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            87..701
                     /locus_tag="MB219_002655"
     CDS             87..701
                     /locus_tag="MB219_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCC8428316.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(725..1075)
                     /locus_tag="MB219_002656"
     CDS             complement(725..1075)
                     /locus_tag="MB219_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCC8428317.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1069..1332)
                     /locus_tag="MB219_002657"
     CDS             complement(1069..1332)
                     /locus_tag="MB219_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCC8428318.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          457 a          264 c          228 g          476 t
ORIGIN      
        1 agacacgacc gcgacattga aaaatctggt tttctcgtgt atgattaggg tgttttttaa
       61 atagttgcag aaagtaggta aaaattttga gagttgcata catacgtgtc tcatccatcg
      121 atcaaaatga acaacggcag attgaagaaa tgaaaaaatt cggtgcagag agaattttca
      181 ttgagaaaca atctggcgca acgattactc atcgacctgt ttttcaagaa gcactggatt
      241 tcgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattacg
      301 acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgcagctg atcatcacaa
      361 gtctaccgat catggccgaa gcaattggca atcctctcct ggataaattc attaaagatt
      421 tgatcattca aattttggcc atgatcgctg aacaagaacg taccgaatct aaacgacgtc
      481 aggcacaagg cattaagatt gcaaaagcga acggtgttta taaaggacgt cctaagctat
      541 acagtgccaa tgcaaaagat ccccaacgtc gtcttgttta taaaaacatc gttgaggatc
      601 taaaaaaagg tgttgcgatc gctaagattg caaaagacta caatgttacg agacaaaccg
      661 tatatagaat aaaaaaagac agcatggtca atcacgaata gttgatcatg ctgctgtttc
      721 tattttattt gcttactttt tgaaaaactg ttctaacttt catcaataac tcctcaacta
      781 aactatcatg cgctttttcc aaaaaaatac actctctggc ttcataatca attgtcgtta
      841 gttgatctaa taatactttt cctgttgtct tcatttcata ttctgagttt atatttacat
      901 atagcggata gtttctagtt gtactagaaa ttggagcaac tgtaacaacg ttactataat
      961 ccgcaataat atcatgactt aaaacaatat aaggtctttt acctgtctgt tcgtgtccag
     1021 atcgaggtgc tgtattcaat aatagaatat cgccttgtct aggttttctc accatttttc
     1081 attacctact gcttcaccta aattttgaat cgtcacatga tttctttcat cagtgtaatt
     1141 ttcaaataac tgttcaattg ttaattcatt ttctatatta gccgcttcga taattagccg
     1201 cttctgaccg tttacttctt cttgtattat cttcactttg tcttctgtat gaattccaag
     1261 atattccata atatactttg gcaatctaag gccattacta tttccccatt tttttatagg
     1321 taacatttcc atagtagaca cctctttctg tatatacata gtataacata cataagaaaa
     1381 aacgtatata cttaattatc cttttctatt aaattagatt aacat
//
LOCUS       DAHEYX010000302         1422 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHEYX010000302 DAHEYX010000000
VERSION     DAHEYX010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.310"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(52..124)
                     /locus_tag="MB219_002658"
     tRNA            complement(52..124)
                     /locus_tag="MB219_002658"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Gln,seq:ctg)
     gene            complement(170..598)
                     /locus_tag="MB219_002659"
     CDS             complement(170..598)
                     /locus_tag="MB219_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428319.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(658..1416)
                     /locus_tag="MB219_002660"
     CDS             complement(658..1416)
                     /locus_tag="MB219_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCC8428320.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          409 a          268 c          272 g          473 t
ORIGIN      
        1 aaaaagattg ttcttttaca gggggttcat tttttcagca agcacatcaa attgggatgg
       61 cagggttcga acctacaatt gcttgattca gagtcaagtg ccttaccagt ttggctacat
      121 cccatttatt caacaaaata atcatagcac tttcctaaaa aaacgcaagt tatttttctt
      181 gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac tagtttcttt tctaatccag
      241 caggaagggc aatcagttct gctactgaca agacggtgtc caataactga tattctttga
      301 caggttcatt cataatatga tagatgtttg ctaaacgttc aatatattca aagcgagtat
      361 actctgttcg agcaattaga aatttcaata aatcggtggc tttctcgtac tcttgatctg
      421 cgtaataaac aagagattga cgatacaatt tgatttccag ttcattgagg gtagcaggtc
      481 tgcgacgctc gttccactca ttaatagaga ctttaccttg aacatttccc ttgcagtcac
      541 ggacaaaaat aatcggaatt tcatttggat ccattttttt atgtgtgaat ttttccatta
      601 tctacgctcc cttcaaaggt ttggtatctt gagtataagg gatttttgtt tgaattacta
      661 gcttttctcc aaagttagca tctgtccacc cattgcagtg aaaatcgccg aaaaatcgtt
      721 gatcggtact gctcgatttt ttacaccata gggatcgttg acataaatat tttctttgtc
      781 atatccagtg actacacaag catgaacaag aggagtgact cttacttctc cattatttgt
      841 ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa ttgatagtca cagaagtcca
      901 gacaggattt cctttagata gtgctttttt gatatcatcg agttgagcat cggtttttgg
      961 aaaagtgtga taatccgttt ggacgatttg ctgagcaagg gaggcaactg ggggaacata
     1021 gacgaccata gcccagtctc cgccactgat atcaccaaca aatccttcat taggatcgcc
     1081 atgagtccca tctgcattga aaacagggac gtaatccaat tgctcagcta actggttttt
     1141 agttgtttca taaccataaa aagataaaag catagaaagc gaagtgactt cacagccatt
     1201 ttctaaagaa ggagaatcaa actggctctc taatggaaca tctagtaaaa cttctgcttt
     1261 ctcttttgtc tttttcgttt tttgtgttga acttttttgt gcagtgttca ttgtatgttc
     1321 tggagaatgg atatgtaggt aataacttgt accgcttaat aaaagagcag ctgtgccccc
     1381 tagaaacaat agttttttga acgttttttt cttcatttga aa
//
LOCUS       DAHEYX010000303         1420 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHEYX010000303 DAHEYX010000000
VERSION     DAHEYX010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.311"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            170..1375
                     /locus_tag="MB219_002661"
     CDS             170..1375
                     /locus_tag="MB219_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8428321.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
BASE COUNT          562 a          230 c          241 g          387 t
ORIGIN      
        1 tgtagataca ccattagaaa gcgatcaata aaaaaagcac atttaccgcc cgaccaagaa
       61 cgcaaatgtg caaagagcta cggtaaagta ggtctatttg ttgtacccta ttttaccatc
      121 tcgaaatccg ctgtacaacc gaacaaacgt acgaaaggaa agataaaaga tggcaaaaaa
      181 agaaattgat aaacgaatta aaaagcactt aacaaaaaaa ggagaagaaa aatatcagtt
      241 tagtctgtac ttaggagttg atccgctaac aggaaaaaaa agaaaaacaa cgagacgagg
      301 ttttagtacg ccattagccg ctgagagagc tttaaaaaga ttggaggcag atatacagga
      361 aaaaggactg caagcttcta cagctcaaaa gagcaagaaa ttcgaatatg tttacggctt
      421 atggtttgaa aattataaaa agacagttaa agaaagcact tggtcctcca ccaaacaaat
      481 ttttgacaca cacatcttaa aagtgtttgg agataagttc attgacaaaa tagacgtttt
      541 tttctgtcaa gaagcagtaa acacttggtc cgatagccat cctaaaattt ttaagaaaat
      601 aaaaaattac acatctaatg tttttgatta cgctgcttct ttacaaatta taactagcaa
      661 tcctatgaaa ttagtttcta ttccacgtgg agaagctcta gatatcgaag ataaaaatat
      721 cgaattttat actaaagaag aattaatcga atttttagaa gcaataagaa acgatgatga
      781 tgaaagatat ttattctttt ccctattggc ttttacaggt ataagaaaag gagaagcttt
      841 tgcactgacc tggtcggata ttgattttaa gagcaaaaca ctcaatatta ataaaactgt
      901 aacaagaggt tatcaaggga gattgatcgt aaatactcct aaatcaaaat ctggaaaaag
      961 aaaaatttat ctggacaatg atttgatcag tttattgaga aagtattata caaaaaacaa
     1021 aacgatcgtt acaatccaaa gcgaaaactt aatttttcac catgatggtc ttctctataa
     1081 tcctacagtt tcacgttcgt ggcttaatgt catatataag catcatccag aattaactaa
     1141 aagaatcact actcatggtt ttcgacatac tcatgcctct ctcctttttg aatcaggagc
     1201 ttctttaaaa gatgttcagg aacgtttagg gcatgcagac attcaaacta cttcaaatat
     1261 ttacactcat gtaactgaaa ctcaaaacaa aaaagtcatc aataattttg tagcatttat
     1321 gaagaatagt actcaggggg agtcaaaagg ggagtcaaaa aacattttag gctaaattac
     1381 aacaaaaaaa gacacgatcg aaatcgtgtc taatcgttga
//
LOCUS       DAHEYX010000304         1364 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHEYX010000304 DAHEYX010000000
VERSION     DAHEYX010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.312"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            82..>1364
                     /locus_tag="MB219_002662"
     CDS             82..>1364
                     /locus_tag="MB219_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCC8428322.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAHEYX010000305         1352 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHEYX010000305 DAHEYX010000000
VERSION     DAHEYX010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.313"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(15..422)
                     /locus_tag="MB219_002663"
     CDS             complement(15..422)
                     /locus_tag="MB219_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428323.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(441..1259)
                     /locus_tag="MB219_002664"
     CDS             complement(441..1259)
                     /locus_tag="MB219_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCC8428324.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          395 a          228 c          190 g          539 t
ORIGIN      
        1 ccgcatgaaa aaaattattt tggtaatttt tcaaactctt cttgtatttc ttctcttatg
       61 tgttcttctt ttgctactcc tagtctatgc tggatatatg agtctgctaa tgtattaaca
      121 attttttcga aagaaattct ccctttttca ctttgtaact ccgtcatata gtcttgtaaa
      181 attgagagtt tgctatttaa tgccggagag atctttttag gaactgatac cttgtttctt
      241 ttatcaggaa ttgaaactgg tcggcctctt tttttcaatg taatatttct tgtgttactc
      301 aatgcactat tgctaaagtt atattcaccg cttattttca catcttggcg tttgttattg
      361 ggtaataatg gctcaaattc accattctcc atcattttag gaatatcaaa atctttaggc
      421 attacttatg caactcctta ttatttattt aaaaactctt gtctagcatc tagttctgcc
      481 aaaatattta taaatacttg gtgtgcttta taatcccatt gatcaaaatt tccatttttg
      541 tattttctta aatgtatacc aattttagaa taccttctca atcttttttg ataatgaatg
      601 agatttttgg ataccacggt tccgtatttt tcaattgctt cttctaggat ttcttcatca
      661 aaatcatcat ttggttctac catacaaggt acaatatcta taatttgcaa atcaagatcg
      721 tatctatcga ccatgaattt ttgataattt acatatttgt tgactccctc taaggattct
      781 tcagctgttt gaaatgaaat tatcgaatag tctgaagctg ccatagcatt atcggaaaat
      841 tcgctaatgg ttggtggtac atcaataaat atttcttgat aattgttctt tattggaaga
      901 agtaattttt tcaagacagt aacttggtct aattcatttt catgtttatt agcaaaagcg
      961 gagaatgatc gaaaactagt gtcacatgcc agaaggtcaa gattttcatt taccttaata
     1021 atactacttg ctaattctcc atttgcgaca ccatttgtta tagtggtttt gatatcggtt
     1081 ataccgaaag ttcttgctat gacttgtgtt aagttcgctt gagggtctaa atcaataata
     1141 agcgtttttc tatttcttat cattgcattg tcataagcaa gcatgctgac tatcttagtt
     1201 ttacctactc ctcctttaaa attgccaact gtaaatgtag tcccttgaaa agtatccatg
     1261 taaatcctcc caatctttaa ttgataatta aagtataact tatatatcgg aaatggtcaa
     1321 cattaaaagt ataacttaaa taataatatc ta
//
LOCUS       DAHEYX010000306         1341 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHEYX010000306 DAHEYX010000000
VERSION     DAHEYX010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.314"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            66..1298
                     /locus_tag="MB219_002665"
     CDS             66..1298
                     /locus_tag="MB219_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCC8428325.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          443 a          252 c          301 g          345 t
ORIGIN      
        1 gaatcataat taaatggtat catgctaata gttaccagaa aaaatggtga ggaggaattt
       61 ttattatgtt atctgatttt aatgaacttc taaaaaaata tgcacgctta attgcagaaa
      121 ctggcgtagc cacagaaaaa ggacacacag ttgttttaca gatcagcgtg gaacaggcac
      181 cccttgctcg attgatcaca caagaagctt ataaactagg agccgctgag gttatcgttc
      241 aatggactga cgaccagatc caaagagaat ttcttttaca tgctgcaacg gatcgtattg
      301 agaatgtacc acaatctaaa atcgatcaag cggatgaatg gcttgaaaaa ggagcaagca
      361 ggatcagcgt tgtttctgcc gatcccgatg catttgctgg tgtagatagc catcgagtag
      421 cgtcttatca atcagcgaat ggcaaagcac taatgaattt acgcaaggca actcaagcaa
      481 ataaagtcag ttggactgtc gtggctgcgg ctggtaaaca atgggcagca aaagtcttcc
      541 cagaactgcc agaagaaaaa caagttgagg cattatggga tcagattttc aaaacgactc
      601 gtgtctatga agaagaccct gtcttagcat ggaagaagca tgatgaaaaa ttagcaaaaa
      661 aagcagaaga attgaatcga gaacaatttt ctgcccttca ctatactgct cccggtacag
      721 atatcattat cggattgcct aaaaaccatc tttgggaagg cgctggaagc tataatgctc
      781 gtggagaaaa attcatggca aatatgccga cggaagaagt gttcacagca cctgacagtc
      841 atcgagtaga tggttacatt tcaagtacaa agccactgag ttatgctggc accatcatct
      901 cgggtatgaa atttactttt aaagatggaa aagttgttga tttttctgcc gaacaaggag
      961 aagatgtctt agcaaaactg cttgatacag atgaaggtgc gcgtcgttta ggagaagtcg
     1021 cacttgtacc tgacccttca cctatttctc aatctggcat catctttttc aatacattat
     1081 ttgatgaaaa cgcatcgaat cacttagcac taggttctgc ttatgccttc agtgtgaaag
     1141 gcggtacaga aatgtccgat gaagaattag cagaagctgg attgaataga agtcaaaccc
     1201 acgtagattt catggtagga tctgacaaga tggatatcga cggaatccgt gaagatggct
     1261 ctacagtacc gattttccga aatggcgatt gggcttgagt atactttcgc acacatgcaa
     1321 aaaataatat aaagcataga a
//
LOCUS       DAHEYX010000307         1316 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHEYX010000307 DAHEYX010000000
VERSION     DAHEYX010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.315"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..440)
                     /locus_tag="MB219_002666"
     CDS             complement(<1..440)
                     /locus_tag="MB219_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCC8428326.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYEL"
     gene            664..>1316
                     /locus_tag="MB219_002667"
     CDS             664..>1316
                     /locus_tag="MB219_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCC8428327.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYT"
BASE COUNT          452 a          215 c          194 g          455 t
ORIGIN      
        1 agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa tgtttcatcc
       61 atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc tttgattcct
      121 aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata aaaataaatt
      181 gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca ttccgaatgt
      241 ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct ttctcttgca
      301 atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg ttgtgcagcg
      361 attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa actaggaacg
      421 aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt taatttattg
      481 ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt tgttttgtca
      541 acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa aatcgtttat
      601 ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa aggagtaatt
      661 catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa agatttttat
      721 ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat acgccgagat
      781 gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt tgaacgaaag
      841 gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt agccgaaaaa
      901 aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat ttatgtcggt
      961 gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct atttgtaatt
     1021 acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg gattttactt
     1081 actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc agaaagatct
     1141 tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt tgataacaat
     1201 gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa tgcagcaaaa
     1261 atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta tacttt
//
LOCUS       DAHEYX010000308         1300 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHEYX010000308 DAHEYX010000000
VERSION     DAHEYX010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.316"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..1250)
                     /locus_tag="MB219_002668"
     CDS             complement(<1..1250)
                     /locus_tag="MB219_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCC8428328.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDK"
BASE COUNT          476 a          279 c          213 g          332 t
ORIGIN      
        1 tttttatcat aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc
       61 atgatcaaga taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct
      121 ggtaatgtcc aagttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa
      181 taactgataa tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc
      241 aacatcatag gagctccaaa aatcatgggc tcattgatat tgaagatacc aggaataata
      301 gaaaaacgac caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat
      361 accacgatcg ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga
      421 gcgatatagg gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc
      481 aacatgatca gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca
      541 aatgaccaca acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct
      601 aaaatattgg ttaaaggctc gaataacgat aataaggtag cgtttacatc aaagcccgcg
      661 acgcttcgaa tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa
      721 ctacgggcaa cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta
      781 aagaaacgat atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat
      841 gctcccaaag aactcatagg taagacccag ccaagaggtg ccttttcagg agttaaaacg
      901 tttacaggaa cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta
      961 tctaatccat atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc
     1021 aatcccatcg tcatcctaaa aggtaaaaca atcgttgccg aataaggggc aataaattca
     1081 ttccatccag gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat
     1141 gacaatgttg aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt
     1201 gccattggtg ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact
     1261 ccttttcaat aaaatatgaa ccattcattg catgacaacg
//
LOCUS       DAHEYX010000309         1279 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHEYX010000309 DAHEYX010000000
VERSION     DAHEYX010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.317"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            5..403
                     /locus_tag="MB219_002669"
     CDS             5..403
                     /locus_tag="MB219_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCC8428329.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="MB219_002670"
     CDS             387..1082
                     /locus_tag="MB219_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCC8428330.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          484 a          192 c          258 g          345 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaa
//
LOCUS       DAHEYX010000310         1255 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHEYX010000310 DAHEYX010000000
VERSION     DAHEYX010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.318"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            27..1130
                     /locus_tag="MB219_002671"
     CDS             27..1130
                     /locus_tag="MB219_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCC8428331.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          407 a          209 c          297 g          342 t
ORIGIN      
        1 attttttata gagggaaggc attgaaatgg aaaaaacgta tcaagcaggt acaaatgaag
       61 gaatagtgga ttttatcaat atggaagatt tagaaatagc cgcttcacaa gtgattccag
      121 ctggtggtta tggttacatc agcagtggtg ctggcgactt atttacttat caagaaaatg
      181 aacgggcatt caatcatcag ctgattattc ctcatgtatt aagagatgta gaattacccg
      241 atacaactac acattttgac aaagaaacgc tgactgcacc aatcatcatg gcaccagttg
      301 ctgctcatgg cttagctcat gtgaaggcgg agaaagcttc tgcaaaaggt gtagcagatt
      361 ttgggacgat ctatacagca agttcttacg cttcttgtac attagaagag ataagagaag
      421 caggcgggga gaaagcaccg caatggtttc aattttatat gagcaaagat gatgggatca
      481 atttggatat tcttgaagtg gccaagcgta atggagcaaa agcaatcgta ttgacagcgg
      541 atgcaacagt tggcggaaac cgtgaaacag accgcagaaa tgggtttacc ttcccactgc
      601 caatgccgat cgttcaggct tatcaatcag gagtaggcca aacgatggat gctgtgtaca
      661 aatcttccaa acagaagctt agtccaaaag atgtagaatt tattgctgct cattctgatt
      721 tgccagtcta tgtgaaaggt gtccagtcag aagaagatgt ctatcgttca ttagaatctg
      781 gtgctggcgg aatctgggta tctaatcatg gtggccgtca actagatggt ggtccagcag
      841 cgtttgattc gttgcaatat gtggcagaag cagtagataa acgtgtacca atcgtgtttg
      901 acagcggtgt acgacgcgga cagcatgttt tcaaagcaat cgcatcaggt gcagatcttg
      961 ttgctattgg cagacccgtt atttatggat tgtctttagg aggaagtaca ggagtccatc
     1021 aagtgtttga tttctttaaa acagaattgg aaatggtcat gcaattagct ggaacacaaa
     1081 cagtagaaga tatcaaaaaa ataaaattgc gtgagaatcg ttttatttaa gaaacaaatg
     1141 acgaaaaaaa taggagtgcg gcagatgatg ccgtactcct atttttatag ttcaaggatg
     1201 tcactaatag aataatttga aacgaaaaaa taaaattgaa atattttttc atact
//
LOCUS       DAHEYX010000311         1246 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHEYX010000311 DAHEYX010000000
VERSION     DAHEYX010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.319"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            200..742
                     /locus_tag="MB219_002672"
     CDS             200..742
                     /locus_tag="MB219_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428332.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="MB219_002673"
     CDS             898..>1246
                     /locus_tag="MB219_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCC8428333.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          237 g          354 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAHEYX010000312         1236 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHEYX010000312 DAHEYX010000000
VERSION     DAHEYX010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.320"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(636..977)
                     /locus_tag="MB219_002674"
     CDS             complement(636..977)
                     /locus_tag="MB219_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCC8428334.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(977..1207)
                     /locus_tag="MB219_002675"
     CDS             complement(977..1207)
                     /locus_tag="MB219_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCC8428335.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          370 a          212 c          179 g          475 t
ORIGIN      
        1 taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg
       61 acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa
      121 actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca
      181 aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa
      241 actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga
      301 tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg
      361 tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag
      421 atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc
      481 tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat
      541 cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta
      601 gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg tataatctga
      661 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
      721 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
      781 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
      841 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
      901 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
      961 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
     1021 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
     1081 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
     1141 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
     1201 caagcattat catcactccc ttaataaaag tatact
//
LOCUS       DAHEYX010000313         1215 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHEYX010000313 DAHEYX010000000
VERSION     DAHEYX010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.321"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(61..144)
                     /locus_tag="MB219_002676"
     tRNA            complement(61..144)
                     /locus_tag="MB219_002676"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Leu,seq:cag)
     gene            672..1172
                     /locus_tag="MB219_002677"
     CDS             672..1172
                     /locus_tag="MB219_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8428336.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          398 a          211 c          206 g          400 t
ORIGIN      
        1 atatgttatt ttctttctca tcgctccagg tgtaccttct ctttataaaa aaacaaatta
       61 tgcggctaag aagactcgaa cttccacgag gtcaccctca ctagcgcctg aagctagcgc
      121 gtctgccaat tccgccatag ccgcatcaca tcttttagtt taggattaca tgtgtttttt
      181 gtcaagaaca tatttttgtt ctttttcatt tctgtacgcc ctcccttatg ccatctacac
      241 aaataattca tccgagcaac tccatcatct attgagggtt ggtgaaaaaa tctgttttat
      301 ccatatgatc gatttcaatg ttaaacgaac gactctggga ttagtaacta ttttattttt
      361 ttcttcattt cttttttcct ctttttctcg attcagctat cttctttcat ttggatattg
      421 attatttctt ctgttgatta ctatccttct ggaattctag acgctgaagt aattcacgtt
      481 ttctttcttc tagagatcat aagtaacgaa tgaacgaact tcaaataatt tttttattgt
      541 aattcatatt tgtattgttc gatgattctt aatacgatat gacacaataa ataaaaatag
      601 gcatggctat ttacaacaat agtgctatag tagaaaacaa atgaataagg ttacttaaag
      661 aggtgaatac gatgaaactg atcaaactat gggaagcaga tttaaagaaa gcttatcagc
      721 tacaaactag ctttggacca aatgagagtg gatttatcaa caatgcttat ggctatacat
      781 ttgctgaatt tttagattat gtaaaaaaat gcgaaaaaac gtctttagga atcgatcttc
      841 ctgagagtta cgttcccgca accgtcttta ttttagaaag tgatgaacat gagtatgtag
      901 gaatttttaa tttgcgccat aaactgaacg aaacattaag aaacggcgct ggccatattg
      961 gttacggcat ctcccctaat tatcgtaaaa aaggatatgc cacacaagga ttagcattag
     1021 tactacaaga ggcaaaaaaa atcggcattg atgaggcact tttgtctgta aataaaaaaa
     1081 atcttggcag tcttaaagca caacaaaaaa atggggctgc gatttatcgt gaagatgaga
     1141 aagaatacta tacgaaaatc aaactgatgt aaaaaagaag gattccaaaa ggaaagggat
     1201 ttgaatgtcc tttat
//
LOCUS       DAHEYX010000314         1192 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHEYX010000314 DAHEYX010000000
VERSION     DAHEYX010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.322"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>1192
                     /locus_tag="MB219_002678"
     CDS             <1..>1192
                     /locus_tag="MB219_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCC8428337.1"
                     /translation="EWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNLETFQH
                     SAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRWFAFPR
                     GVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVTPAIQNFQSKKNNKH
                     KVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNTTFENL
                     TMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLITAILVK
                     TTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVGKFIRA
                     VRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGYAQVYG
                     KYVS"
BASE COUNT          390 a          200 c          223 g          379 t
ORIGIN      
        1 gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt gtattaaatg
       61 tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg agaaacttag
      121 aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg aatattttgt
      181 taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt actgttattg
      241 gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga tggtttgcat
      301 ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt agtgtttacc
      361 ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc cttatcgtgg
      421 gtttggagaa agatgtcaca ccagctatcc aaaattttca atctaagaaa aacaataaac
      481 ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa ttgatcgatg
      541 atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc aacatttatc
      601 aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag aatctgacaa
      661 tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct tctgggttca
      721 gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt tcgcttattt
      781 tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa acaacttcac
      841 ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa ttttcaatct
      901 ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc ttggctaaaa
      961 gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt tttgacgaat
     1021 tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca aggccagaaa
     1081 gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta agacacaatg
     1141 tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca ga
//
LOCUS       DAHEYX010000315         1190 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHEYX010000315 DAHEYX010000000
VERSION     DAHEYX010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.323"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            98..313
                     /locus_tag="MB219_002679"
     CDS             98..313
                     /locus_tag="MB219_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428338.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            350..712
                     /locus_tag="MB219_002680"
     CDS             350..712
                     /locus_tag="MB219_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCC8428339.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            758..1084
                     /locus_tag="MB219_002681"
     CDS             758..1084
                     /locus_tag="MB219_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428340.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT          452 a          143 c          256 g          339 t
ORIGIN      
        1 aggaactttg agatattctt taggatatta aaattaacta gcaccacggg tgaaatatat
       61 catattgttg tatataataa aacaggaggg gagaatcatg aaaaaagtaa gtaatctcag
      121 ccgatacaga aaagaaaaag gactttctca aacggagtta gcaaaaaaaa tgaatgttac
      181 acagcaatgt gtaagttcgt ggcaaacagg gagaacgatt cctaaaccct atcaaatgaa
      241 gatgctttct gagatattaa gtgtaccaat gaatgagctg ttttctgatg tatttaataa
      301 agtaaacagt taactaataa gcaattgaat gattttagga ggagaagaca tgtattgtag
      361 aaaatgtggc aaagaaataa cagatgaaag taagtattgc cagttttgtg gagcactaca
      421 agaaacagat gagacaaccg gaacagacac agtaaagaaa agtactgagc agacaattaa
      481 tgaaatgaag gaacaaatcg tacatggaca agagacaggg aagctgtatt tgattattgg
      541 ttggatttct atggtagtat cgttagcatt tattcctgtt gtatttggtg ctgttgctgt
      601 tattatgggc tacttatatc gagaaaaaga tgagaaaatg ggtacgattt tgatgatagc
      661 aggggtagct ggagcaatct ttggtgtact tttagggatg tcaatgtact gataagaggg
      721 ataagaacaa attattttgg agataggaga taaaaagttg aataaagtaa atatgaacaa
      781 gaagtttgtc attataggta ttgttgtctt ggctattata ggtattattt tttcaaaaat
      841 gacaggtaca tctggattag agaagtcatc aaaaccattg gtaacacaga ttttacaaga
      901 acagtacaac ttaaatcgat catgtgatga tgtgacgatt actcatgaaa atggagataa
      961 cacataccgg gctaaagcta tattagacaa tggaagtgct atcaacataa atattgagta
     1021 ctatccaaag aaagaccgta tatatgtgga gataccatat gctgaggtgt taatgttaaa
     1081 ttgatttaaa tgtattatgc ataccgtcca aacaggaact cccaaaggtg taaattcact
     1141 agggagtttt tatagtttcc tataaattaa atactaatac aggtgacaaa
//
LOCUS       DAHEYX010000316         1184 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHEYX010000316 DAHEYX010000000
VERSION     DAHEYX010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.324"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            132..1064
                     /locus_tag="MB219_002682"
     CDS             132..1064
                     /locus_tag="MB219_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428341.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
BASE COUNT          426 a          166 c          222 g          370 t
ORIGIN      
        1 attatttgaa aaataaagaa atcattatcc cagaatttga ctcgcggaac atcttgctag
       61 ctttagtacg ttatgtcaat tgggaaggct atgaaaataa aaatccgtat gtagaagaag
      121 cttgtaacga gttgattgat tatgtagaga ctcatttttt taaaagaaga tattcggaag
      181 atgaaaagtc ctttatcata cgaggaatag agatcgcaat cggaagaaag gcatttcccc
      241 ttggactgaa agaagaagat aagaaaagcg cagaaacgaa gccgttattt caattagtct
      301 gtcaaggatt ggaagagcaa aaaggaattc tagacttaca agaagaagat gtctattata
      361 ttttttcttt gttcaacagt agaaattata ccaatgagaa catggagctg ttaagaaaag
      421 atttcgaagt tgtttataaa aattttgttc tcaatgacct atcactgaat gaattgattt
      481 tacagattat tagtatcgct gaaatcgacg atgcagaaaa ttttattttc aagcaatcct
      541 ttttacaatt tgtccgtacg ttgtgggcag atggacaagt atttttaccg gaaaggatct
      601 atcttctatc agaaaaagaa caacttctgt acggaaaaat tgtagaaata ttggaagagt
      661 ggaaaaagaa aaatcaaatc caactaagat ggaatgaaaa tttcattcga aaattcgtca
      721 aaagtctttc tttgatcaac agtgaacaag cggggagaca ggaaatagaa atttttgttg
      781 tttcagaaag ttcagtcaaa caattttttt atcgaaccca atttcatttg tatgcggaag
      841 aaggcatcgc tgaaataaat cctattttat ttcgttcatt agaagaattg cctgatgaat
      901 gtctatgtgc gaaaaaaaga gtggttttgt gcgatgctgc tagctatcaa aatggattag
      961 aaactgaaaa gacaaagatc tatccaatct cattaaaagg tgcaaacatc catttgtatc
     1021 atattgctca ggaacttcac ctattaaaaa aacatatttc ttgatgagac cttaattagg
     1081 agaccttcta caaagctttt taattgtctg tagaaggttt ttatttgagt cctgattcgt
     1141 gcgaagatct acttatataa caagctttct ctgaaatttt ttta
//
LOCUS       DAHEYX010000317         1163 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHEYX010000317 DAHEYX010000000
VERSION     DAHEYX010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.325"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..358
                     /locus_tag="MB219_002683"
     CDS             <1..358
                     /locus_tag="MB219_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCC8428342.1"
                     /translation="LEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGD
                     IAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISS
                     FNILRRNPKELLIDNN"
     gene            complement(644..>1163)
                     /locus_tag="MB219_002684"
     CDS             complement(644..>1163)
                     /locus_tag="MB219_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428343.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          368 a          205 c          214 g          376 t
ORIGIN      
        1 attagaaatt tttggtcaat tcctaattga gatggtattt atatcaatcc ccgcattcgt
       61 tggttcctat ttcttagctc aatatacagc tgacaagctt gggaataata tattgaataa
      121 ggttactggt gatatagcta aacaaatagc taaacaatct gcatctagcc aattaggtgg
      181 tggggcagaa gctgaaggat ttaacaagac attatcaagt ttagatatca atgtattacc
      241 taaattcatg atttatgtag ttatatttat gagtttagta cttcttgtat cattgattat
      301 ttcttcattc aatatattaa gaaggaatcc aaaagaatta ttaattgaca ataattaaaa
      361 ttttcggtgc tttttagcac caaaaatgag agtgtaatat aaaccgtgta agtaaccatc
      421 aatcctaggc gggtggttgc ttcacggttt ttatttttct aaaataagat caagaggtga
      481 ttacacgatg gctagaaaga aaagaaatcc tgatgctgag aagttagctg aatccatttt
      541 gaatacttat caacccgaat ctgtagagga tatgcaagat gttttaaaag atgtgtttgg
      601 tcccttattt gaaaaaatgc ttcaaggaga attgaataac catttagggt atgacgccca
      661 ttctaaagag ccaaagaaac atgacaatcg acgaaacggt tatggcaata agacgcttaa
      721 aacgagcttt ggtaaagtaa acattgatgt tcccagagac cgggaggctt cttttgaacc
      781 tgagttgatt cctaatagaa aacgagatgt ctccgacatc gcagactgta gacaaaagct
      841 gttccaaact cgtaactcga gcttggaaca gctttttatc tacttcgttt cgaatttttg
      901 aacaaaaaaa taaaggctcc ttgcctttcc atagccaggt ggctaatttc tttaaattca
      961 ttgcagcaaa cactagcatc gtatgcgtcg cgactttttc caagcctcga tacttggtcc
     1021 aacgcatccc atgcttttct tttgcatccg caaatattcg ttcaatcgtt tgtttccgtt
     1081 ttttgtaaat tgatttattc agatctgtta ggcgaagatg ttccacttca tccatcagat
     1141 ctccccaaac atgtctcgtg atg
//
LOCUS       DAHEYX010000318         1160 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHEYX010000318 DAHEYX010000000
VERSION     DAHEYX010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.326"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            44..895
                     /locus_tag="MB219_002685"
     CDS             44..895
                     /locus_tag="MB219_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCC8428344.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            912..1157
                     /locus_tag="MB219_002686"
     CDS             912..1157
                     /locus_tag="MB219_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428345.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT          399 a          228 c          232 g          301 t
ORIGIN      
        1 gcgctacact tatagttgaa cgaataaaaa aggagctgaa agtatgaatt cattaacaag
       61 cacttatcgt ctatcaaatg gttacgaaat cccggtagta ggattcggca catggcaaac
      121 acctgatggg gatgtcgctg tttcctctgt taaagaagcg ctggctgcag gttatcgcca
      181 tatcgatact gcccaaggat acaagaatga agaaagcgtc ggccaagcga ttaaggaaag
      241 tggaattcct agagaagaga tattcttgac aacaaaatta tggaatgcaa accacagcta
      301 tgaacttgtg atgtcttctt ttgaagaatc attgaagaag ctccaaacag attatttaga
      361 tctgtttttg atccactggc caaaccctgt tgcttttcgt gataattggg aacaagcaaa
      421 tgcagataca tggcgagcat ttgaagaact gtatgaagca ggtaaaatca aagcaatcgg
      481 tgtgagcaat ttcttacccc accatctaga tacactagca aaaacagcaa aaattatgcc
      541 gatggtcaat caagttttcc ttgcaccagg cgaattacag acaactgtag ttgagtatgc
      601 aaaaaaacat gatatgatcc tcgaagcata tagtccatta ggcactggta aaatcttcga
      661 tgtgcctgaa atgaaacaaa tcgctgaagc acatgacaag acgattgcac aagttgcttt
      721 gcgttggtcg ctgcaacacg aattccttcc tcttcctaag tcagtgacac caagcagaat
      781 caaagaaaat actgaattat ttgattttga attaacagaa gaagaaatga aacaaatcga
      841 tcagttagat ggtgtagtcg gcaaagcaaa agacccagat actacccaat tttaagtcga
      901 aaggtgattt tatggatcca atcgaacgct tgaattctct ctcggaagaa gttacccaga
      961 cttttcattc cgattttgtc tttttgatcg atgcagagaa aatccaacat tttcctgcaa
     1021 gaaactggac acatgatcaa atcatcgaag aactgaaaaa acggttcgat cattcgctaa
     1081 tggtcaaacc ctggcacgaa catgaagtca tttattcacc tgaactacct gtttttgcct
     1141 tgataccgaa aaaatagaaa
//
LOCUS       DAHEYX010000319         1155 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHEYX010000319 DAHEYX010000000
VERSION     DAHEYX010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.327"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            7..1077
                     /gene="xerS"
                     /locus_tag="MB219_002687"
     CDS             7..1077
                     /gene="xerS"
                     /locus_tag="MB219_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCC8428346.1"
                     /translation="MYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFY
                     EWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLS
                     TSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARANAIEKK
                     LFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSEVVNIN
                     LEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIY
                     QGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHTS
                     TNTTTLYTHIDNAATIDALNAL"
BASE COUNT          463 a          193 c          180 g          319 t
ORIGIN      
        1 acagaaatat atcaaaaaat agatacaatc atacttcata tgcccgatta tataaaagat
       61 tttgttcaag atcgtgagga taaagatcaa tcacctcgaa ctactttaga atatctaaaa
      121 aattataagc tcttttatga atggcttctg agtgagtcta tcgttcccga tacttcaatc
      181 acctcgataa gacagttaac tgcctatgat ctaaacttgt acaaatctca tttgaaacgt
      241 cgcacaaagg aaaatgtaaa aaaagagaca acaaaaacta agctcaaaga taataataac
      301 ttaggtctat ctacttctac tatcaataga aatatcacag cattaaaggt tctatttaaa
      361 tatctttcga aaagttccaa taatccttta ggaaaaccat acttagaaaa taatcctatg
      421 gatcaagtag caactatcac agataaaact acgttagctg cccgtgcaaa cgcaatcgag
      481 aaaaaattat ttctagacga agatacacaa aattatctag attatattgc caatgaatat
      541 aaaaaaactt tgagcaaacg agctttgatc tattatcaac gagatgtaga aagagacctg
      601 gcgatcaatg cattgatttt aggatctgga ttaagactct ccgaggttgt aaatattaat
      661 ttagaggacc tatctttaga aaagaataat gtggtagtaa ctagaaaagg aaataagaga
      721 gacgcagtaa atatagcagc atttgcaatg gaatatttag ctaattatct agctattcgg
      781 aaagaacgat acaaagtaac agaaaacgaa aaagctttat ttctagcaat ttatcaagga
      841 gaagccaagc gtatatccgg tattgcaatc gaacggatgg tagcaaaata ttcaaaagga
      901 ttcagagtcc aagtcagtcc acataaatta cgtcatactt tggctactag actctatcaa
      961 cagacaaatt cattggtatt aactgcccaa caattgggac atacaagtac aaatacaaca
     1021 actttatata ctcatataga caatgcagct acaattgatg ctttgaacgc tttgtaaatg
     1081 tagtaaaaaa tagtataata atgaagcctg tctctaaaat tagataaaag cccaacttta
     1141 aaggcaagct tcatt
//
LOCUS       DAHEYX010000320         1154 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.328, whole genome shotgun sequence.
ACCESSION   DAHEYX010000320 DAHEYX010000000
VERSION     DAHEYX010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.328"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            45..320
                     /locus_tag="MB219_002688"
     CDS             45..320
                     /locus_tag="MB219_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428347.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            747..>1154
                     /locus_tag="MB219_002689"
     CDS             747..>1154
                     /locus_tag="MB219_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCC8428348.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNL"
BASE COUNT          434 a          200 c          174 g          346 t
ORIGIN      
        1 gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa aaaaatgaaa cttgaggata
       61 aaaaagtcat tttgaagaaa atcaaacgac taccagacca aatagatggc tatacgatct
      121 gttctttcaa aaaagacagg agtataaaag cttatacgca aaacacagaa aaaggaataa
      181 tctatttcct acatgaagaa ggttacgata cccaaacctt tatgtttact gaaaaaaaag
      241 aatttcataa acagatgaaa aaactgattg aaaaagaatt tccaagaagt cataaactct
      301 atgtgaatca gcaattttaa cttctaactg ttctttatta attaaaaatg ttctgtaata
      361 aaataaaact ttataacaac tataacaaaa ccgaatgcaa aacagaagtt ctataagtta
      421 taatcatagt tgttaggtga ttcatctagt aaaaacacca gatttcaaat ttcacacaat
      481 ttaaactaga tcaatcatca accttcttca aattgttaga accctttccc gaggagctgc
      541 agcataagca aaaaaacaaa caggaagcca aatggctaag acattttttc tattagtcta
      601 gttgtttctc gtcgcaagtt taagacttta gaaagttatt tctaatgatg tttgggttgt
      661 tttgatcaat gcttatgcac agctccttac ctattattta tctcaacaga tctaacaaat
      721 taatggacta tttaaggagt ttttttatgc ttacgatcta ttcttctcgc acacatagtt
      781 accatctgat ggcaaactgg ctgaaagacc atcaaatgcc ttacagagaa cgaattttca
      841 cacctaaaca gcctttaaca aggaaagaga tattttattt attgtcatta acagaaaacg
      901 gatttgacga tctattagct aaacgctcga ttgattataa aaatttgaaa gaccaaatcg
      961 accaatgtac gacctcagag cttgtcgatc tgatcgtctc gcattttact ttgttacaac
     1021 gtcccatcat tactgacgga aagaaattat tagtcggatt caatgaagat agcatgcgtc
     1081 tttttatccc tgtacaatgg cgcagaagtc aactgaaatc tgtctataac gattttacat
     1141 gggtaagcaa tcta
//
LOCUS       DAHEYX010000321         1152 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHEYX010000321 DAHEYX010000000
VERSION     DAHEYX010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.329"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>1152
                     /locus_tag="MB219_002690"
     CDS             <1..>1152
                     /locus_tag="MB219_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCC8428349.1"
                     /translation="LVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRD
                     VLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQ
                     LNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIV
                     EFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSE
                     ISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKG
                     VILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAI
                     VTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTT"
BASE COUNT          430 a          165 c          199 g          358 t
ORIGIN      
        1 aattggtagg aagactcaat gacaatatag ctagtaatcc tgaagaagac gatgaagctc
       61 tgttgggctc aagaattgac atgtcagata ttttcaagca tatttcggtt gtttatgttg
      121 acgctcttcg agatgttctt agagaaatga aaaacaatcg aaacccagtc aaaagaatca
      181 tggaaacaat tgaatcaaaa atatcatcag ataatgttga gtcattaaaa acaattatac
      241 agcaattaaa tgagactatt accagtgttc ctgaaattag gaaaattggc gaaaatatta
      301 atcggcaact aaatagcata attggttcag tgtattctcc taatcttctt ctttcctcaa
      361 ctatgtcaga tgatatgggt tctttagcaa aatttctttc aatgaagcca gaacaaaata
      421 tcgacttaga cttactaggg ttaggacatc taaatatgat ttatttagct ttgaaaatag
      481 tcgaatttga agcgtgtcgt tcaagagaac tacttaatat tatgttgatt gaagaaccag
      541 aagctcatat tcatcatcat attcaaaaaa ctctgttcga aggattaaat cttcaaaaaa
      601 actatactca gattctcatg accacacatt ctgtacattt agctgaagtg tctgaaatat
      661 ctcggatgaa tgtattaaaa tcgtttaatg gcaaatctat cgttatgaat ccagcaaata
      721 atctgaactc atttgctaaa gaaaagttaa aaaagaacaa tctaaatcta ataaacgcag
      781 tagagagata tttagatgtt aaaagaaatg gattgctttt ttctaaaggt gttatcttag
      841 ttgaaggaga tgctgaagaa atacttattc ctcaaatagt aaaaaaatta tttggtataa
      901 acttagacga acttggaata ggtttgatta atgttggtag tacttcgttt gaatatatcg
      961 ctagcttgtt tgacgatgat agaatacaga gaaagtgtgc tattgtaact gatttagatg
     1021 aacaggcaat cccatcagaa catcgattgt ataaaagcac tgcacaagag aaaggtcaag
     1081 caagaaaaga aaaattatcc tcccttttta atgaaaacaa ttgggttaat tgtttttatg
     1141 ctaatacaac at
//
LOCUS       DAHEYX010000322         1145 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHEYX010000322 DAHEYX010000000
VERSION     DAHEYX010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.330"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>1145)
                     /locus_tag="MB219_002691"
     rRNA            complement(<1..>1145)
                     /locus_tag="MB219_002691"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          237 a          352 c          229 g          327 t
ORIGIN      
        1 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
       61 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
      121 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
      181 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
      241 ttctcacttc taagcgctcc agcagtcctc acgatctgcc ttcaacgccc ttagaacgct
      301 ctcctaccaa tacacctaaa ggtgtactcc acagcttcgg taatatgttt agccccggta
      361 cattttcggc gcagggtcac tcgactagtg agctattacg cactctttaa atggtggctg
      421 cttctaagcc aacatcctag ttgtctgtgc aaccccacat ccttttccac ttaacatata
      481 ttttgggacc ttagctggtg gtctgggctg tttccctttc gactatggat cttatcactc
      541 acagtctgac tcccggatat gaatgaatgg cattcggagt ttatctgaat tcggtaaccc
      601 gagatgggcc cctagtccaa acagtgctct acctccatca ttctcaattc cgaggctagc
      661 cctaaagcta tttcggagag aaccagctat ctccaagttc gtttggaatt tctccgctac
      721 ccacacctca tccccgcact tttcaacgta cgtgggttcg gtcctccagt gcgttttacc
      781 gcaccttcaa cctggacatg ggtagatcac atggtttcgg gtctacgact acatactcaa
      841 acgccctatt cagactcgct ttcgctgcgg ctccgtctct tcaacttaac ctcgcatgca
      901 atcgtaactc gccggttcat tctacaaaag gcacgccatc acccattaac gggctttgac
      961 ttgttgtagg cacacggttt caggttctat ttcactcccc ttccggggtg cttttcacct
     1021 ttccctcacg gtactggttc actatcggtc actagggagt atttagcctt gggagatggt
     1081 cctcccggat tccgacggaa tttctcgtgt tccgccgtac tcaggatcct cctaggtgtc
     1141 ttcca
//
LOCUS       DAHEYX010000323         1143 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHEYX010000323 DAHEYX010000000
VERSION     DAHEYX010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.331"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            27..500
                     /locus_tag="MB219_002692"
     CDS             27..500
                     /locus_tag="MB219_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCC8428350.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            509..736
                     /locus_tag="MB219_002693"
     CDS             509..736
                     /locus_tag="MB219_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428351.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          364 a          189 c          196 g          394 t
ORIGIN      
        1 ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta aaatttgtcg
       61 ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact cagatcgcta
      121 caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct gtcatttact
      181 ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg aaaaaaatcg
      241 aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc gaacgaacca
      301 ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa gtagcggatt
      361 tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt ccttttgcag
      421 tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta cttaagacag
      481 atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag atgtagtatt
      541 agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta cgatggcaaa
      601 catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc tcgaaaaaat
      661 ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg actcgaatac
      721 tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat ctgaatgata
      781 gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta tttcgattga
      841 ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc cttttcttct
      901 ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag ttggatctgc
      961 ttttagtata atactttatt cattccacca gactgatagc agtttgagca gtctaaataa
     1021 tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac ttcagctaat
     1081 ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat atactgtaaa
     1141 gcc
//
LOCUS       DAHEYX010000324         1120 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHEYX010000324 DAHEYX010000000
VERSION     DAHEYX010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.333"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..1004
                     /locus_tag="MB219_002694"
     CDS             <1..1004
                     /locus_tag="MB219_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCC8428352.1"
                     /translation="KKLNTLVTIENPFRVKQFSRMFDENKTDRNDALRIADFLRIQRF
                     TTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFS
                     ATLISLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGA
                     LAQESINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGL
                     IAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSV
                     KNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          400 a          219 c          195 g          306 t
ORIGIN      
        1 taaagaagct caacacactt gtaacgattg aaaatccttt tcgggtgaaa caatttagtc
       61 gcatgtttga tgagaataaa actgatcgaa acgatgcatt aagaatcgct gatttcttgc
      121 gtattcaacg attcacaact tcccctatca aagaagagaa atacatggcc ttacagcgtt
      181 taacacgtac tcgctatcaa cttattaagc aattgattcg tacaaagcaa cattttcttg
      241 aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa tcgacgagtc
      301 tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat gaacttgctg
      361 agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc cgtttcaagc
      421 aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac cgcctaggtg
      481 ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa atacgagcac
      541 ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt gtcattccag
      601 aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc ggtttaatcg
      661 ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa tacgctggat
      721 taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct ctgacaaaac
      781 aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta aaaaattatc
      841 ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag catcaacaca
      901 aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg ctactacgta
      961 aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc gttattgacg
     1021 caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt tcgtgcgctt
     1081 caaaacatat tactcaataa agaaaatctt aaatttcatc
//
LOCUS       DAHEYX010000325         1110 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHEYX010000325 DAHEYX010000000
VERSION     DAHEYX010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.334"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..905
                     /locus_tag="MB219_002695"
     CDS             <1..905
                     /locus_tag="MB219_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCC8428353.1"
                     /translation="YFTACGGSQAVLMAGQYLFDKELTVPCHVYTANEFNYAIPKGLS
                     ENSLVITCSHSGTTPETVEATKKAREFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDV
                     DASDLNKGILYGLLFNILLVLDGSKKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKN
                     QKRDKIIYTIGSGINYGEIYSTAACWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFI
                     LVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQ
                     MVEAIAFERGHSLDVRRYMWQMKY"
     gene            887..>1110
                     /locus_tag="MB219_002696"
     CDS             887..>1110
                     /locus_tag="MB219_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCC8428354.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHH"
BASE COUNT          382 a          148 c          214 g          366 t
ORIGIN      
        1 tatattttac agcatgtggg ggttcacaag ccgttttaat ggctggtcaa tatctatttg
       61 ataaagaatt aactgtacct tgtcatgttt atactgctaa cgaatttaac tatgctatac
      121 caaaaggatt atctgaaaat tctttagtta tcacttgttc ccattctggc acaacaccag
      181 aaactgtaga agcaacaaaa aaagcaagag aatttggagc aatgactatt tgtttatcta
      241 atgaagaagg atctcctcta tgggaagcag ctgaatatcc tgttcactat gattggggta
      301 aagatgttga tgctagtgat ttaaataaag gaattcttta tggtttatta tttaatattt
      361 tacttgtttt agatggctct aaaaaatggg aaacttgctt gaatgaattt gataagttaa
      421 atgatcttgt tattgctgaa aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa
      481 accaaaaacg agataaaatt atttacacaa ttggtagcgg tattaattac ggagaaattt
      541 actccacagc agcttgctgg ttcatggaaa tgcaatggat taattctaat gctattcatt
      601 ctggggaatt tttccatggt ccttttgaaa tcactgatta tgatgtacca ttcatcttag
      661 tgaaaagtat tggagataca agatttttag atcaacgggt agaagacttc actaaaaaat
      721 tcagtaataa attaatggtt ttagaccaag ctgattttga cttagaatct atagatagtg
      781 tagcgaaaga atatattgca gctgtactta gtggcgttgt tattcgccaa atggtagaag
      841 cgattgcttt tgaaagaggc cattccttag atgttagacg ctatatgtgg caaatgaaat
      901 attaattaat aggaggttgc aaaagatggc aaatcaatta tttcacattg tgattacaac
      961 acattctacc ctttgtgagg gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc
     1021 cacaggtatt agtgctgttc catttaaaga aaatactagt ccagatgatt ttgaggagag
     1081 tatgaaagaa ataattagtc agcatcattt
//
LOCUS       DAHEYX010000326         1096 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHEYX010000326 DAHEYX010000000
VERSION     DAHEYX010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.335"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            191..445
                     /locus_tag="MB219_002697"
     CDS             191..445
                     /locus_tag="MB219_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCC8428355.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="MB219_002698"
     CDS             446..763
                     /locus_tag="MB219_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCC8428356.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          386 a          170 c          220 g          320 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaat
//
LOCUS       DAHEYX010000327         1084 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHEYX010000327 DAHEYX010000000
VERSION     DAHEYX010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.336"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MB219_002699"
     CDS             195..>1084
                     /gene="aph(2'')"
                     /locus_tag="MB219_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCC8428357.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIR"
BASE COUNT          410 a          157 c          204 g          313 t
ORIGIN      
        1 aaatggccat tagaagatag gaacttagca gatactgcac tttggctaaa tgcagaattt
       61 atgaaaaatg cagcagagat aggttatgca agatttttat atggaaatag catgaaacaa
      121 aaatgaatta actataattg ctaaattctt atttacagtc aatcagattt ttgattaaag
      181 ggagtagaga agacatgaca acttatactt tcgaccaggt agaagaggca atagagcagt
      241 tatatcctga ttttactatc aatacaatag agatttcagg agaaggcaat gactgtattg
      301 catatgaaat aaacgggaat tttattttta aatttccaaa gcattcaaga gcttcgatta
      361 atctcttgaa tgaagtaacc gtactcaaaa caatccacaa tgaattatca ctacccattc
      421 ccgaggtggt ttttacagga atgccatcag aaatgtgcca aatgtctttc gcaggtttta
      481 caaaaattaa aggagtacct ttgacacctc ttctactcaa aaatctgccg aagcaatctc
      541 aagatcaggc agctaaggac ctggcccgat ttttaagtga acttcacagc ataaatatct
      601 ctggattcaa aagtaatctg gtattagatt ttcgagagaa gataaatgaa gataataaaa
      661 aaatcaaaaa gttactatcc agggaattaa agggtcacca gatgaagaaa gtggatgatt
      721 tttacaggga tattctagac aacgaaatct acttcaaata ctatccttgt cttattcata
      781 acgatttcag cagcgatcat attttatttg ataccgaaaa aaataccatt tgtggaataa
      841 tcgattttgg agatgcagct atttctgatc ccgacaatga ttttataagt ttgatggaag
      901 atgatgaaga gtacggcatg gaattcgtat caaaaatatt gaaccattac aaacataagg
      961 atataccgac agttttggaa aaatatatga tgaaagaaaa atactggtcg ttcgaaaaga
     1021 ttatctatgg aaaggaatat ggttatatgg attggtatga agagggatta aatgaaatca
     1081 gaag
//
LOCUS       DAHEYX010000328         1075 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHEYX010000328 DAHEYX010000000
VERSION     DAHEYX010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.337"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(64..134)
                     /locus_tag="MB219_002700"
     tRNA            complement(64..134)
                     /locus_tag="MB219_002700"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:Cys,seq:gca)
     gene            complement(180..251)
                     /locus_tag="MB219_002701"
     tRNA            complement(180..251)
                     /locus_tag="MB219_002701"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Gln,seq:ttg)
     gene            complement(263..335)
                     /locus_tag="MB219_002702"
     tRNA            complement(263..335)
                     /locus_tag="MB219_002702"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:His,seq:gtg)
     gene            complement(338..411)
                     /locus_tag="MB219_002703"
     tRNA            complement(338..411)
                     /locus_tag="MB219_002703"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(377..379),aa:Trp,seq:cca)
     gene            complement(418..498)
                     /locus_tag="MB219_002704"
     tRNA            complement(418..498)
                     /locus_tag="MB219_002704"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(462..464),aa:Tyr,seq:gta)
     gene            complement(500..575)
                     /locus_tag="MB219_002705"
     tRNA            complement(500..575)
                     /locus_tag="MB219_002705"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(540..542),aa:Phe,seq:gaa)
     gene            complement(611..683)
                     /locus_tag="MB219_002706"
     tRNA            complement(611..683)
                     /locus_tag="MB219_002706"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(648..650),aa:Asp,seq:gtc)
     gene            complement(693..765)
                     /locus_tag="MB219_002707"
     tRNA            complement(693..765)
                     /locus_tag="MB219_002707"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Val,seq:tac)
     gene            complement(781..852)
                     /locus_tag="MB219_002708"
     tRNA            complement(781..852)
                     /locus_tag="MB219_002708"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Glu,seq:ttc)
     gene            complement(867..955)
                     /locus_tag="MB219_002709"
     tRNA            complement(867..955)
                     /locus_tag="MB219_002709"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(919..921),aa:Ser,seq:gga)
     gene            complement(995..1068)
                     /locus_tag="MB219_002710"
     tRNA            complement(995..1068)
                     /locus_tag="MB219_002710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Asn,seq:gtt)
BASE COUNT          302 a          284 c          243 g          246 t
ORIGIN      
        1 tggattttga gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta
       61 ctaaggcggt aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac
      121 ttggctatac cgccattata ctattatcaa tctacaaaat aaagaataaa ttatagaaac
      181 tggggtagct ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg
      241 gctatacccc aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc
      301 acaatccggt gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa
      361 cccacactaa cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg
      421 aggagagtgg attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag
      481 ccacttcgct actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct
      541 tcaatccgtt gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata
      601 acaagaacga cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat
      661 gttaacccct acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac
      721 cctctgcttg taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa
      781 tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt
      841 gaccaacggg ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt
      901 tgcaccatct acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt
      961 gaaagaagct caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg
     1021 acatcatgat taacagtcat gcgctactac caactgagct atgccggaat aacat
//
LOCUS       DAHEYX010000329         1070 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.338, whole genome shotgun sequence.
ACCESSION   DAHEYX010000329 DAHEYX010000000
VERSION     DAHEYX010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.338"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            25..>1070
                     /locus_tag="MB219_002711"
     CDS             25..>1070
                     /locus_tag="MB219_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCC8428358.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     MSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DAHEYX010000330         1067 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.339, whole genome shotgun sequence.
ACCESSION   DAHEYX010000330 DAHEYX010000000
VERSION     DAHEYX010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.339"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..478
                     /locus_tag="MB219_002712"
     CDS             <1..478
                     /locus_tag="MB219_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCC8428359.1"
                     /translation="DNVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNG
                     EYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQHTELADIV
                     KTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            545..1039
                     /locus_tag="MB219_002713"
     CDS             545..1039
                     /locus_tag="MB219_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCC8428360.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
BASE COUNT          384 a          143 c          214 g          326 t
ORIGIN      
        1 tgataatgta ttagtggata ctattttagt accacttcca gagaattacg aagtaattaa
       61 ctttagtcag ttgaaaatta gtgatgtggt ttcacaagct attgaagacg ctgagtcttt
      121 tatgagtaat ggtgaatatc aacgtgcctt tgacagagtt cacactgctt ttcatggata
      181 tttaattgaa atactaaaaa aatatgaaat tacagttcct agagacgaaa atctatctaa
      241 attatatagt aggatacaac aattaattga aaaagaaatt caacatactg aacttgctga
      301 tatagtgaag acaacgataa ggtcatcaaa tggaatgatt agctctctta atgaagcaag
      361 aaataggcat tcacttgcac accctaatac aaatattatt ggaaagagag aagcaaaact
      421 aataataggt atttcaagta cagttacaga ttatattagc ggataccttg ataagtagcg
      481 tcattaactg attgctactt tttttatgac tatttttata aaaaggatgt gataagaatt
      541 ggaaatgaaa gtctatgtgg caaatttagg acggtataat gaaggtgaat tagtcggagc
      601 atggtttact ccaccaattg atgaggaaga aatggcagaa agaatcggtt taaatgagga
      661 ctacgaagaa tatgcaatac atgattttga gcttcctttt gatgtggacg aatacacgcc
      721 gattagtgaa atcaaccgtc tatgtgaagc cattcaagaa atcgaaggta cacctattta
      781 taatgaactc aaagaaattc aaggcatgtg gtttagtagc ttggaagaat tactggaaaa
      841 caaagaggat attcattgtt atagtgattg tgattcgatg gaagatgtcg cccgttatta
      901 cgtggaagaa acaggacagc ttggtgaagt cccttccaat ttacaaaatt atattgacta
      961 ccaatctttg ggacgtgata tggaaataga gggtaattac ctagttacct ctcatggggt
     1021 atttgaatat tgtcagtaag tttaacaatc cgtgttataa ttaaatt
//
LOCUS       DAHEYX010000331         1064 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHEYX010000331 DAHEYX010000000
VERSION     DAHEYX010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.340"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            1..797
                     /locus_tag="MB219_002714"
                     /pseudo
     CDS             1..797
                     /locus_tag="MB219_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            complement(914..1064)
                     /locus_tag="MB219_002715"
                     /pseudo
     CDS             complement(914..1064)
                     /locus_tag="MB219_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT          374 a          190 c          184 g          316 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaaggacga gttatttaca caaaattatt
      841 gacgctccca ataaaaaaac aaacaaagta aaataatcta ctttgtttgt ttttatttta
      901 attaatattg aatttataaa attgtcttat cgcttggttc cacaaccaag tagcgataag
      961 gctcatcccc ttcagaatga gtggtaacat aaggattttt acttaaaaca gaatgaattt
     1021 gttttctttc aaaagcgggc attggttagt caaagactag ttca
//
LOCUS       DAHEYX010000332         1045 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.341, whole genome shotgun sequence.
ACCESSION   DAHEYX010000332 DAHEYX010000000
VERSION     DAHEYX010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.341"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            107..>1045
                     /locus_tag="MB219_002716"
     CDS             107..>1045
                     /locus_tag="MB219_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCC8428361.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRET"
BASE COUNT          348 a          158 c          239 g          300 t
ORIGIN      
        1 acgatagagg attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg
       61 gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc
      121 agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc
      181 ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta
      241 ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt
      301 tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc
      361 taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt
      421 cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga
      481 ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga
      541 cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca
      601 aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg
      661 ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca
      721 ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg
      781 ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg
      841 aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat
      901 cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg
      961 agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt
     1021 tcctgaacta gttatcagag agaca
//
LOCUS       DAHEYX010000333         1044 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHEYX010000333 DAHEYX010000000
VERSION     DAHEYX010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.342"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..174)
                     /locus_tag="MB219_002717"
     CDS             complement(<1..174)
                     /locus_tag="MB219_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428362.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEET"
     gene            complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MB219_002718"
     CDS             complement(276..629)
                     /gene="tnpB"
                     /locus_tag="MB219_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCC8428363.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(619..813)
                     /locus_tag="MB219_002719"
     CDS             complement(619..813)
                     /locus_tag="MB219_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428364.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          301 a          211 c          181 g          351 t
ORIGIN      
        1 cgtttcctca aagagagtct ctttagagcg tccaaataat ttgttggtta aataattgac
       61 cttttcattc aatagttgga tttcagttgt aagattctga atggtttgtt gttgtgcttg
      121 attggtttta tgggcttctt gaagcaactg aagcaacgta tctgtagttt ccaaaatttt
      181 tcaccacctt gaatcgaact gattttgtta cagaatacca caagaaacgc acaagaaata
      241 ggggttcaac acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc
      301 aaaatagctc gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc
      361 gttaaggttc gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc
      421 ttataacaga gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg
      481 ttcccgcaaa ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata
      541 agactggcaa gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata
      601 tgtttggttt gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag
      661 aatataattg ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg
      721 catcgctaac ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa
      781 ttggacagga acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct
      841 caagtatatc ttgaggtgca tttgttagta aggtactgtt ctatggggcg cttaccttgt
      901 gtcttcctaa aaaggtcttt tcctgtttct atgtaaaaca aaaagagctg caatcctcat
      961 ttaggactac aactcttctt ttatttatat gcccatcgat cgcgtaaaat gatcgggatt
     1021 tctaacatac atgctagcaa taac
//
LOCUS       DAHEYX010000334         1037 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.344, whole genome shotgun sequence.
ACCESSION   DAHEYX010000334 DAHEYX010000000
VERSION     DAHEYX010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.344"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..645)
                     /locus_tag="MB219_002720"
     CDS             complement(<1..645)
                     /locus_tag="MB219_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCC8428365.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLD"
     gene            779..>1037
                     /locus_tag="MB219_002721"
     CDS             779..>1037
                     /locus_tag="MB219_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428366.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGV"
BASE COUNT          384 a          184 c          173 g          296 t
ORIGIN      
        1 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
       61 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      121 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      181 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      241 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      301 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      361 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      421 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      481 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      541 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      601 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      661 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      721 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      781 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      841 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      901 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
      961 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1021 tcctctttat ggagtga
//
LOCUS       DAHEYX010000335         1008 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHEYX010000335 DAHEYX010000000
VERSION     DAHEYX010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.345"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..746
                     /locus_tag="MB219_002722"
     CDS             <1..746
                     /locus_tag="MB219_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCC8428367.1"
                     /translation="GNLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRN
                     LENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQ
                     ENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEI
                     ANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSS
                     HFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          378 a          113 c          178 g          339 t
ORIGIN      
        1 caggaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta tcacaaaatt
       61 acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat gttaaaaatg
      121 gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata attccaagtg
      181 ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt gaaaagttag
      241 cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt agagatgctt
      301 tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg agggattctg
      361 ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt tctcccttaa
      421 tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact gtaaaggaaa
      481 tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag aatgaaatgc
      541 atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa ataatgttga
      601 aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat tcttcacact
      661 tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag
      721 tggataattt caacaacatt agctgagagg ttttatttta ttatgagact atatcattta
      781 cgtagtttgg aggagttgag gaaattggat tttaacaaaa aattttttgg gaaatttctg
      841 atttgagttg tttcgtagaa tctgagcgtc taagtaatgg taataatagt gtgttaaatg
      901 aatatgacaa agtagtaatt aataaagtaa aaagtttatg tgaagaagct agagaatcta
      961 tcagtcatat taatggtagc aattttaagc aaacattatt taatctga
//
LOCUS       DAHEYX010000336         1002 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.346, whole genome shotgun sequence.
ACCESSION   DAHEYX010000336 DAHEYX010000000
VERSION     DAHEYX010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.346"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            364..834
                     /locus_tag="MB219_002723"
     CDS             364..834
                     /locus_tag="MB219_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCC8428368.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
BASE COUNT          349 a          153 c          216 g          284 t
ORIGIN      
        1 agctctttca ctaggacgag acccgaatca ctcgataatt gaccacctgt atgagaaata
       61 gtgatatttg aattgaattt tacttggttt ttgtgtaagc tagtcatgaa aagaactcct
      121 ttcttttggt tggtttagtc actttaacca tagcagaaag gggttctttt ttcatcactt
      181 aacgggtgaa gatgaaaaag taattgtaag ctgccgtgag gcagtttcac atggttttaa
      241 ttaaaagtat gaattattca ggtagaatct ctgaagaata aatcacgtga tcgtgacgat
      301 aaaatacact tacgtcaggt gataaaaaat acataagtag aaaactaaga ggaggaacag
      361 aaaatgaaag aagttcaatt gatcagaaaa agcgaactat cagaaggtgg ttgcaatgca
      421 tgcggcgtag tagaagcaac aagctataca ttaaagcttg gtgccaacaa agcgataatc
      481 tctgagttga cagtaggcgg tcttgtggac agtctggcgc ttgctgaagg ttttattgga
      541 gaagatattt atgagatgtt ttcagaaatc cgtcagttaa aaaaagggga aaactgcata
      601 gaggttcatc atgagagccc gaatgtgaga ttcaaacgtg gtgataatga gatgatcttc
      661 aataatcatg tgtccgatca caccgagcta tatggaatcg tgaatcaaat actaactgag
      721 ctttttggat tgggacctta tgcgttcaag gaagaaaacg gtaatccaaa attaaacgaa
      781 gaatggcaag aaacgattga aatacaaaga aacaacccgc acttatttca ataggcatcc
      841 acagtatttc gtaaaaggga ctttgacacg acttttgtca aaatcccatc gttttttatc
      901 tatttgtgtt ctaaacattt ctttcagagt agtaacaact ggaatgactg aaatgtaaac
      961 agattatttg cctgtatcaa tacggaagag taatataaag tg
//
LOCUS       DAHEYX010000337          998 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.347, whole genome shotgun sequence.
ACCESSION   DAHEYX010000337 DAHEYX010000000
VERSION     DAHEYX010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.347"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            98..838
                     /locus_tag="MB219_002724"
     CDS             98..838
                     /locus_tag="MB219_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428369.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          367 a          178 c          195 g          258 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
      901 tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt
      961 ttctcccaat aagtaagaaa ataatggcaa aataaaaa
//
LOCUS       DAHEYX010000338          991 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHEYX010000338 DAHEYX010000000
VERSION     DAHEYX010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.348"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            398..988
                     /locus_tag="MB219_002725"
     CDS             398..988
                     /locus_tag="MB219_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCC8428370.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          327 a          153 c          179 g          332 t
ORIGIN      
        1 gatatgaatg tacgcagaat ccttgttttc tacggcattc gctgtttttt cattggacta
       61 gttatatatt tctagggttg ttttcatgtc tgagtgcccg atatgattta tttttcgttt
      121 ctataattac tttttgcctt ccagtttctt ttaagattgt actcaagcaa gtgggaattg
      181 gttcttttgt catccatgta tgcaaaatgt tataatgctg gtaaacaaaa atgattacct
      241 gtcattttta tttagttcgt atttaattaa gttgactgtc tgtatactct gtctaaaact
      301 taagaattgt tgttttcgct gtcgctatcc aaatttaatg aatgtctgta tgttaaaact
      361 gttgtccttg tcatagatag taaaggagag cttgaaaatg aaaactgcga ttacgtttct
      421 attagaggaa tatgccgatt gggaaggtgc ttatattact agcacactaa accaaaaaga
      481 gggatggcaa aacaaaactg cttctatcca atcagaggtc acttccattg gaggattaaa
      541 aacaaaaatc gattacttaa tcgaacaaat acctgaaaaa gttgaccttt ttattatgat
      601 cggtggcaat tcatgggaca taaaaaatca tcatttatat caaataatcg ataaattgtt
      661 aaaaacagac gtaccagtag ctgctatttg tggggcagtt gattatttag ctcagagcgg
      721 attattgcaa ggttacaaac acaccggaaa tgcgcaattt ccttggttag aatcggaaaa
      781 ttatcagaat aaagaagatt ttatagaaca acaagctgtg aaagatcgta atttgattac
      841 tgcgaatgga acagctccta tagaattcac agacttgatt cttcaagcag tccattttga
      901 aacagaagaa ataagagaaa aaaagattcg tctatacaaa cttggattct atgattacta
      961 taaaaagtac ggagatcctt tctcctaatc a
//
LOCUS       DAHEYX010000339          985 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.350, whole genome shotgun sequence.
ACCESSION   DAHEYX010000339 DAHEYX010000000
VERSION     DAHEYX010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.350"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..916
                     /locus_tag="MB219_002726"
     CDS             <1..916
                     /locus_tag="MB219_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCC8428371.1"
                     /translation="YSKMKDSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLM
                     DKLNCYQGKVKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQN
                     SFDDDLSETIDKSTSSRNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDT
                     ETDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHK
                     AEELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIE
                     KAIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          415 a          150 c          158 g          262 t
ORIGIN      
        1 atactctaaa atgaaagaca gctcaaaaat agcctatatg ctttttaaaa atgaatttcg
       61 tagagcctta catgaaaaat ggatcgacga tgatggtcta ttatatttgg aatttacaca
      121 agcagaccta atggacaaat taaattgcta ccaaggcaaa gtaaaaacca ttttaaacga
      181 gttagaaaca tataatttga tttcagtcgt aaaaggagaa tttaacccaa aaacgaaaaa
      241 aaatgaaaaa aataaatact atttacttca acctgaaata agtgccgatg atctttttat
      301 tcaaaattct ttcgatgacg atttaagcga aactattgat aaatcaacct cttctcggaa
      361 tgcggaaatc gcacgtcgag aaaaaacagc tgaaaccctt gataacactg gaaatgcgga
      421 aatcgcacaa gattattata ttaataaatc tttagacact aaaagacact taaaagacac
      481 tgaaacagac caattacaaa atcaagtttt gctagataac ttcgttgaaa tcatgaaaga
      541 agacagcata aacacatttg taccagaaaa ggtattaagc ttgataaaaa cattttctaa
      601 cacttacgaa gaagcccaac aaaccgtcac cacaatacac aacgcaaaac ataaagccga
      661 ggaacttgag aagacggtag ttgtgtatga agaacttgaa tcttacggaa ttgatgcgga
      721 taaaggatta tatatgacgt tgctgaaagc ttatcaaaaa caaaagactg agaaagtaaa
      781 taatttgcag aatttgattt ttgtgtatgt aaaaaactgg tttattgaaa aagctattgc
      841 ggcaaagctt gcaaacgaac aaagagaaac atttaacgaa ttaccaacag ttagtacgga
      901 aaattggcta gagtagttga taaaaagcga acactataaa aatgatcaat tttaaattag
      961 aactttttta caattttaat tttat
//
LOCUS       DAHEYX010000340          970 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.352, whole genome shotgun sequence.
ACCESSION   DAHEYX010000340 DAHEYX010000000
VERSION     DAHEYX010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.352"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(53..787)
                     /locus_tag="MB219_002727"
     CDS             complement(53..787)
                     /locus_tag="MB219_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCC8428372.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          334 a          200 c          181 g          255 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc
//
LOCUS       DAHEYX010000341          947 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.353, whole genome shotgun sequence.
ACCESSION   DAHEYX010000341 DAHEYX010000000
VERSION     DAHEYX010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.353"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..105
                     /locus_tag="MB219_002728"
     CDS             <1..105
                     /locus_tag="MB219_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCC8428373.1"
                     /translation="PRLGVFTLESPSGSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(219..848)
                     /locus_tag="MB219_002729"
     CDS             complement(219..848)
                     /locus_tag="MB219_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCC8428374.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          317 a          149 c          159 g          322 t
ORIGIN      
        1 ccacgtctag gtgtatttac tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa
       61 ttagtagatc tacgaccaga aaaattctct ggaataaaac aataactttc tctctattgt
      121 ccataaaact agtctcaatc aagctgtatt gaaaaaacaa gacaacattc ttaccatggt
      181 aaataaaaag acgacacatt ttgtcgtctt tttttaaatt aactattttc gataaaagat
      241 cgcatagctt gtgccattat ttctggcttt tctctattca attcatgttt tccgttttta
      301 ataaagagta gccggccatt cgttgaaagt ttatataaac gttttgctgc tggtttattc
      361 gcatgatctt tggtaccgca taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt
      421 ttgcagtcta atgtttcaag tgaacgaagt agggtaaggg tttgtttttt agatagacct
      481 aatttttcaa atatcttaga aggtacgcag taaaaataaa tattctggaa agtaagcaat
      541 aagcgattag gagactcaaa ttgtggagcg actaaaacaa ccttttttaa atatggagaa
      601 ggagttgcaa gatagtttaa agcaataaca gcgcctaaag ataatccaca taaaataaaa
      661 ggttccttta tctgattcaa ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca
      721 gtgtctgtgc tgtttaaatg attgaacaat gaaagacttt tgcaagaata actattgggt
      781 agataagaga ttacatcttt ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca
      841 attttcatat tgctctcctt tttatagtta tcatatagac tttctttgta ctattaaaat
      901 ataaacattc atttcttacg tcataaaata aaaaaacagc gcagaca
//
LOCUS       DAHEYX010000342          933 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.354, whole genome shotgun sequence.
ACCESSION   DAHEYX010000342 DAHEYX010000000
VERSION     DAHEYX010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.354"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(633..>933)
                     /locus_tag="MB219_002730"
     CDS             complement(633..>933)
                     /locus_tag="MB219_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCC8428375.1"
                     /translation="LMTEVDISILSKIPREYFHPKPRVNSSLIVLKRHPSKISLKDRK
                     QYENFVMKWVNKEYIKLFSKNQFYQALKYARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          311 a          124 c          171 g          327 t
ORIGIN      
        1 gagagatgac tggtaaattt aaaaatgata gaaatggaga gtatgtatga gtaataaaca
       61 atttttatgg tcttcttttg catttattgt tgttgatatt tttttccgat attttaataa
      121 cgatatgata tttaactggc aaagaattat aatcgattac tgtatttttt gtataatata
      181 ttttttagtg aaaacatttc taaaatacgc aaaaaaataa aattgaagat gttattaaat
      241 cgcgattatt tacggagatt tatataaata aaaaccccct gacgaagtcg aagggggttt
      301 ttattttggt ttgaggtttg catacctacc gaaaaggtag ggcagcaaat cgtcaaacag
      361 gtatcagcta attgcaccat aagggtgcgc tgatactcgg ctcaaaagag ccgaaagagc
      421 cgtctgcaag acccctcggc gagcccacac tttgcttgca aagtatagtg ggttatactt
      481 tacatggaag taattgccgg aaatagtgaa aatgcctcac atttgtgcca cctaaaaagg
      541 agcgatttac atatgagtta tgcagtttac gggccaagat tgtttaagca aaataagata
      601 agttaaggga tgcagtttat gcacccctta acttatctat taaataattt atagctattg
      661 aaaagagaca agaattgttc aaagctaata ttgtttaaat cgtcaattct tgcatatttt
      721 aaggcttgat aaaattggtt tttggaaaat agttttatgt attctttgtt aacccatttc
      781 ataacaaaat tttcatattg ttttctatct ttgagtgata tttttgaagg gtgtcttttt
      841 aatacaatta acgagctatt aactctaggt tttggatgaa agtattctct agggatttta
      901 cttaatatgg atatatcaac ttctgtcatt aaa
//
LOCUS       DAHEYX010000343          929 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.355, whole genome shotgun sequence.
ACCESSION   DAHEYX010000343 DAHEYX010000000
VERSION     DAHEYX010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.355"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="MB219_002731"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="MB219_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCC8428376.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          271 a          192 c          139 g          327 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaat
//
LOCUS       DAHEYX010000344          916 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.356, whole genome shotgun sequence.
ACCESSION   DAHEYX010000344 DAHEYX010000000
VERSION     DAHEYX010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.356"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            86..496
                     /gene="spx"
                     /locus_tag="MB219_002732"
     CDS             86..496
                     /gene="spx"
                     /locus_tag="MB219_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCC8428377.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            510..866
                     /locus_tag="MB219_002733"
     CDS             510..866
                     /locus_tag="MB219_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCC8428378.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          321 a          142 c          177 g          276 t
ORIGIN      
        1 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
       61 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
      121 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
      181 ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga ctgaaggagg
      241 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
      301 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
      361 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
      421 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
      481 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
      541 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
      601 ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag
      661 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
      721 acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat accaacctcc
      781 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
      841 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattc
      901 catacagttg taacag
//
LOCUS       DAHEYX010000345          912 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.357, whole genome shotgun sequence.
ACCESSION   DAHEYX010000345 DAHEYX010000000
VERSION     DAHEYX010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.357"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(336..604)
                     /locus_tag="MB219_002734"
                     /pseudo
     CDS             complement(336..604)
                     /locus_tag="MB219_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          274 a          175 c          112 g          351 t
ORIGIN      
        1 aaatcgttga taccaaggtc agactctaga atttcatcag tgagtccctg tattggttaa
       61 ccttcatcaa tcccaaaaaa atatatcaat gcttagcatc aaaatgaata tatagtttag
      121 aatacttggg attcatcgat aactgatcgc ttatttggcc atattatttg gcacttggtt
      181 caactaaacg catttaggat gttccttcat tctcgtcatg gatgttctca aaccgtcatt
      241 actaataaac tgtccaccga atcgatggtt catttcttgc attttccacg tccaattact
      301 ctttttgctc tttatttggg cgtagtttca ctttcttaac ttgtatcgtc ttccatcaac
      361 tccttaatca tcttcttagc tttattggct cggtttcctt gtaaaaggta tctacaaata
      421 gtgacaatct gtattaaatc ttcatcattt tctgtaatgt caataatttc tatattctgt
      481 accatatatt gcaaagggtt tcactaagtt cataaccgaa gcgaattaaa caacttttgt
      541 atagaataac taccttttct atttttccat tggtaattct atctattaat tggtttaatc
      601 cttttgttgt agttgattcc actaccaaga tgattctctc gaattttaaa gcttgaagat
      661 gtattgctat ttttgctaac tggcgtattt gcaactattt ttcccttcta ttcttccttc
      721 attgattccc ctctaaaatt actatccata aaaaattgca taaatgacaa tagtatttat
      781 attagtcata ctgcttttta acacagcaaa ttccagaaaa acattcgtta catatttgtg
      841 aatttttcca aaaaataaca tttttataaa acgacgaaaa ataaatcact accagtcaac
      901 ttctataata tt
//
LOCUS       DAHEYX010000346          900 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.358, whole genome shotgun sequence.
ACCESSION   DAHEYX010000346 DAHEYX010000000
VERSION     DAHEYX010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.358"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(68..229)
                     /locus_tag="MB219_002735"
     CDS             complement(68..229)
                     /locus_tag="MB219_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428379.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            complement(226..897)
                     /locus_tag="MB219_002736"
     CDS             complement(226..897)
                     /locus_tag="MB219_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCC8428380.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
BASE COUNT          258 a          172 c          133 g          337 t
ORIGIN      
        1 atctatttaa tagtcttaat tcattaaaac aaaaaactca actttacaag gatatggttt
       61 aaaaaattta tttatccaac ccaactagtt ttcgctgctt acgatttgct tcatctgcta
      121 cgtacttcat tactgtttta aattctttcg ctgaggcgtt ttctgataaa tcagcgatac
      181 ttttattata cttcttcacg atttgttgta cttgttcttt ctcagtcatg taattcctcc
      241 tccaactctt ttgaagtata ttgtattggg tagtactaat tgaatattat ctgctgcata
      301 atgcacttca gaaatgtcgt tttgataatc cttaatcatt atgtgaagga ctacttcagg
      361 atgtctattt ttgacttttt caatattctc tctggactta aaaaacgcat tgataaacat
      421 ttcttttggc acatgacgtc cttcagctag ttcacgtttt cgagtgaaat cccaggcaac
      481 tttgggatct tggtaaacat agaaaatcgt aacgcgaaag ttatttttaa gggctcgaat
      541 aatatttttc tctgcactaa ggatagcaaa agtatcctct agtatgaaag aatagccatg
      601 ttccagtaca tattttaatg attgttcaac taaccaagaa gaagctttct gaaaaacact
      661 tgagttcttt ccattgtatt ctggaaattg tgctctaaaa tagtccgcat caatcacaac
      721 atggttgtca taattttcag ctaaacctag tgcaacctct gttttacctg ctcctgggct
      781 tccagccatg aataccgcat ctttttcatc atcagggatt tttcccttga tgattgtttc
      841 aataaattta tctttattga ccctagcgta atctaaatac ttgtctttag gttccaagat
//
LOCUS       DAHEYX010000347          853 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.361, whole genome shotgun sequence.
ACCESSION   DAHEYX010000347 DAHEYX010000000
VERSION     DAHEYX010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.361"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            283..522
                     /locus_tag="MB219_002737"
     CDS             283..522
                     /locus_tag="MB219_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428381.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          288 a          159 c          127 g          279 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt ctt
//
LOCUS       DAHEYX010000348          851 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.362, whole genome shotgun sequence.
ACCESSION   DAHEYX010000348 DAHEYX010000000
VERSION     DAHEYX010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.362"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>851)
                     /locus_tag="MB219_002738"
     rRNA            complement(<1..>851)
                     /locus_tag="MB219_002738"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          271 c          188 g          217 t
ORIGIN      
        1 catctcacga cacgagctga cgacaaccat gcaccacctg tcactttgcc cccgaagggg
       61 aagctctatc tctagagtgg tcaaaggatg tcaagacctg gtaaggttct tcgcgttgct
      121 tcgaattaaa ccacatgctc caccgcttgt gcgggccccc gtcaattcct ttgagtttca
      181 accttgcggt cgtactcccc aggcggagtg cttaatgcgt tagctgcagc actgaagggc
      241 ggaaaccctc caacacttag cactcatcgt ttacggcgtg gactaccagg gtatctaatc
      301 ctgtttgctc cccacgcttt cgagcctcag cgtcagttac agaccagaga gccgccttcg
      361 ccactggtgt tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc
      421 tcttctgcac tcaagtctcc cagtttccaa tgaccctccc cggttgagcc gggggctttc
      481 acatcagact taagaaaccg cctgcgctcg ctttacgccc aataaatccg gacaacgctt
      541 gccacctacg tattaccgcg gctgctggca cgtagttagc cgtggctttc tggttagata
      601 ccgtcaaggg atgaacagtt actctcatcc ttgttcttct ctaacaacag agttttacga
      661 tccgaaaacc ttcttcactc acgcggcgtt gctcggtcag actttcgtcc attgccgaag
      721 attccctact gctgcctccc gtaggagttt gggccgtgtc tcagtcccaa tgtggccgat
      781 caccctctca ggtcggctat gcatcgtggc cttggtgagc cgttacctca ccaactagct
      841 aatgcaccgc g
//
LOCUS       DAHEYX010000349          831 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.365, whole genome shotgun sequence.
ACCESSION   DAHEYX010000349 DAHEYX010000000
VERSION     DAHEYX010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.365"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(506..706)
                     /locus_tag="MB219_002739"
     CDS             complement(506..706)
                     /locus_tag="MB219_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCC8428382.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          258 a          160 c          110 g          303 t
ORIGIN      
        1 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
       61 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
      121 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
      181 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
      241 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
      301 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
      361 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
      421 attcagccca ttcaacgacc aatgaatttt ttcgtacaaa aaaaccgtct tttctttcga
      481 aaagacggtt aaaagtcaat ttttattaag ctttaacaat gtttgttgct tgagggccac
      541 gttgaccatc ttcaacatcg taagacactg cttggccttc ttctaaagtt ttgaagccgt
      601 caccttggat agctgaaaaa tgtgcaaata catcatttcc atcttcacca gtgataaagc
      661 caaaaccttt gtctgcgttg aaccatttta ctgtaccgtt attcataaat aaattcctcc
      721 tgatacgtat caatgtacgt tttttcgttg caataaatat ttgataggca aaaggagttt
      781 tatctatgta gatatatcgc tcaaattacg tttcaaaata gattactcat t
//
LOCUS       DAHEYX010000350          825 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.367, whole genome shotgun sequence.
ACCESSION   DAHEYX010000350 DAHEYX010000000
VERSION     DAHEYX010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.367"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
BASE COUNT          319 a          119 c          132 g          255 t
ORIGIN      
        1 agagactacg gttgatttga tattaacaag cgtatatgaa tgtatataca aataaaaaag
       61 gggagggaga agttaaagaa aattcagaga agtggggcac tcagtagtat taacaattta
      121 aaaggaattt cttaaagaaa atgggttgca tcctggagat acaatttttt tagataaaga
      181 aaaaattcaa gcaacaattc aaaaaaatct taactgctga gattgatgta gcaatatctc
      241 aatcatcaac aaagtacgat cctgcctttc aaaagcttat tgataaatgt ttattcatta
      301 taaaaataaa agaaaaattt atgtcagctt ttttcatcaa agtggtaatg ataattcgtc
      361 tattaattgt attttttagg aaatatggta taattgtttt aacgaaaagg taattcatga
      421 tacaccaaaa accactagct ggcgggctag tggttttctt ttacgttcta ttcagattaa
      481 cgggtaggct tactgtcttt tttggcaata attttgcaat caagccattt acagatgtaa
      541 tgcgcggata cttctgctaa gattgcaaaa aacatatcga aaaggtaatt cataatacaa
      601 cctcctcccc ttgccataat aggccggggt agcagacagc ataaatatta taccataact
      661 gaaaatttca ttttagtaca tttcaatata ttgatgaacc agcacaataa aacatgccat
      721 gactaacaga aaaaaactta ttaaaccaga tataacttgt aaacatccaa ctatatagaa
      781 aacgtaaatt ttctatatag ttggattaag ataacgacta tttat
//
LOCUS       DAHEYX010000351          820 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.368, whole genome shotgun sequence.
ACCESSION   DAHEYX010000351 DAHEYX010000000
VERSION     DAHEYX010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.368"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>820
                     /gene="bsh"
                     /locus_tag="MB219_002740"
     CDS             <1..>820
                     /gene="bsh"
                     /locus_tag="MB219_002740"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCC8428383.1"
                     /translation="SYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGIADYPLYYD
                     ATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGETKKLLKNINL
                     ANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLN
                     NYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSE
                     SESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQIT"
BASE COUNT          278 a          114 c          150 g          278 t
ORIGIN      
        1 tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt tcgaaaggta
       61 aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc tgactaccct
      121 ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa tttttctggg
      181 tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt tgaatttatt
      241 ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt aaaaaatatc
      301 aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca ttggctatta
      361 gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca tatatatgat
      421 aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt taatttaaac
      481 aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca aataagtttg
      541 aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc ctcagtatct
      601 cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc agagtcagaa
      661 agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg tttgtgtgat
      721 gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt tgacaaagga
      781 atctattatt atcgaacata tgaagacagt caaattactg
//
LOCUS       DAHEYX010000352          796 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.370, whole genome shotgun sequence.
ACCESSION   DAHEYX010000352 DAHEYX010000000
VERSION     DAHEYX010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.370"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(127..681)
                     /locus_tag="MB219_002741"
     CDS             complement(127..681)
                     /locus_tag="MB219_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8428384.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          243 a          154 c           84 g          315 t
ORIGIN      
        1 tctatctctg gtattctatt gtaaatgtta ctaaacgttt taaatacttc cgaaaattca
       61 gttatcttca ttaatctcac cttctaagtt ttcattatac acaaaaaata tatcctaata
      121 tctcaattag cctcccaatt taaaaccacg taacgatgcc cctatttcat cttcagttat
      181 accaatgtac ctttttgtaa ttgcttgcga gctatgatta aaaataaaca ttaaggtagc
      241 aatgtctcta gttttcttgt agtagtgata cccaaaagtt tttcttaatg tatgtgttcc
      301 tatatcatct cttcctaatt tttctgctat atctgcatat atctgataga cacgatccct
      361 tgttatatgt cgaccttgtt gccgacttgg aaataaccaa tcattttctt ctttaccttc
      421 agtatataat aaaatctctt ctctcaaatt atctataaaa atcggttttc gcttacctgt
      481 tttatgttca acaataatag gcttactttt acctcgtatg tctccaactt ttaatcctat
      541 aatatctgac atacgtaacc cattattgat tccaaacaaa aataaaaaac tatctctttc
      601 tggattactt gttcttctta gccaaaactg aaaatcttgt atctcttcat ttgtccttaa
      661 cggttgtaca ttatagctca ctattgtttc ccttcttaaa aatattctga gttcatgatt
      721 atatatatat tttaccataa tcatgaactt atatatatac tctgttaaaa gtgaagaaaa
      781 tcaaggggtt tagttt
//
LOCUS       DAHEYX010000353          778 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.371, whole genome shotgun sequence.
ACCESSION   DAHEYX010000353 DAHEYX010000000
VERSION     DAHEYX010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.371"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            331..600
                     /gene="rpsN"
                     /locus_tag="MB219_002742"
     CDS             331..600
                     /gene="rpsN"
                     /locus_tag="MB219_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCC8428385.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            615..>778
                     /gene="rpmF"
                     /locus_tag="MB219_002743"
     CDS             615..>778
                     /gene="rpmF"
                     /locus_tag="MB219_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCC8428386.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVI"
BASE COUNT          288 a          107 c          159 g          224 t
ORIGIN      
        1 taaacataaa agattgaata aaaagttata gtttctttca tattgccgtt ttaagtagag
       61 gaaaagctac ttaaaacggt atttgtgttt gattggacta ataaaagtga attcttattt
      121 ttcagagctt aaactgcata ttcttttaaa tgatttgatt atctctatgt accgataaat
      181 tcgttttcaa atagttatct attcatatga gttttatttc atgttattta ttgttgacaa
      241 gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa cgattacgat ttgagatgtc
      301 taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa agtcgaaaat tgctaaagca
      361 aagaaacaaa tggcgatgat tgaaaagtac gcagataaac gtcaggaatt aaaagccgca
      421 ggagatcgca ccgctctcgc taaattgcca agagattcaa atccgaatcg tctgaggctt
      481 cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa agttcggtat gtcccgtatt
      541 aagtttagag aacttgccca tcaagggttg attcctggag taaaaaaagc aagttggtaa
      601 aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc taaggcgaag aaacggttaa
      661 gaagaactca tcaaactgta gtaaaaccag aaatttcatt tgacgaagcg aatggtgatt
      721 atagacgtag ccatcatgta tctttaaaag ggtactataa tggaaagaaa gtaattaa
//
LOCUS       DAHEYX010000354          768 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.373, whole genome shotgun sequence.
ACCESSION   DAHEYX010000354 DAHEYX010000000
VERSION     DAHEYX010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.373"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            29..595
                     /locus_tag="MB219_002744"
     CDS             29..595
                     /locus_tag="MB219_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8428387.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT          268 a          110 c          161 g          229 t
ORIGIN      
        1 actgcagtaa agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat
       61 tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa
      121 tattgctaaa aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga
      181 agatttaaaa aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc
      241 tattgaacgt actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa
      301 aaagcatcat agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat
      361 gcatgatttt tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca
      421 attatcggat gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt
      481 agcgtcattg atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat
      541 caaacggaca attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc
      601 tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc
      661 taaaaaaagc gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa
      721 gtttaaaaat agtacttacg gaaggttggc agagtggtaa tgcaccgg
//
LOCUS       DAHEYX010000355          749 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.374, whole genome shotgun sequence.
ACCESSION   DAHEYX010000355 DAHEYX010000000
VERSION     DAHEYX010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.374"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            15..716
                     /locus_tag="MB219_002745"
     CDS             15..716
                     /locus_tag="MB219_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCC8428388.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          261 a          142 c          133 g          213 t
ORIGIN      
        1 gagaggactt tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc
       61 aacctgattt tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc
      121 ttcgttctta taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt
      181 atatcttgac caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact
      241 actatgacta tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga
      301 cctacactta tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct
      361 actatgaaaa attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt
      421 ctaaagtagt tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg
      481 accgggtaaa acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg
      541 gtcatatcac cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga
      601 cacgtgaaac tgctggacaa gtaatcaaac aactacgtga ttccgggctg ttagattatg
      661 acaaaaagcg ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg
      721 aacaaaaata agcagtgacc ctgataaat
//
LOCUS       DAHEYX010000356          743 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.375, whole genome shotgun sequence.
ACCESSION   DAHEYX010000356 DAHEYX010000000
VERSION     DAHEYX010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.375"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            514..>743
                     /locus_tag="MB219_002746"
     rRNA            514..>743
                     /locus_tag="MB219_002746"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          273 a          113 c          147 g          210 t
ORIGIN      
        1 ctgtaatatt cgtgtttaaa aataataaat aaaaataggg aattttctat tgcaatttta
       61 taaagagatt gctatattat tatttgttct aaatgattcc tttaaggtgt cactaaaaat
      121 tataaaaaat agggttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat
      181 gaggtagcta ataaagcttg attttatcga aaaaaacttc gaaaaagttg ttgacaaata
      241 acaataaaga tgatatgata atcaagtcgc tgctaatgag tgacaaagac gtttagaaaa
      301 aataaacaaa gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttga
      361 aacaaaaaga ttagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
      421 tcttgattca attcaagaca acaaacattt ttaacaagca agcaatatgc tagcaaacaa
      481 attgagctta acaatcgcaa gattgttcga actttttatg agagtttgat cctggctcag
      541 gacgaacact ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt ccaccggagc
      601 ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca
      661 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatca aaaccgcatg
      721 gttttgattt gaaaggcgct ttc
//
LOCUS       DAHEYX010000357          721 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.377, whole genome shotgun sequence.
ACCESSION   DAHEYX010000357 DAHEYX010000000
VERSION     DAHEYX010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.377"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            172..597
                     /locus_tag="MB219_002747"
     CDS             172..597
                     /locus_tag="MB219_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428389.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT          233 a          120 c          136 g          232 t
ORIGIN      
        1 caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat
       61 tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt
      121 tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa
      181 ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt
      241 ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa
      301 gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg
      361 ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga
      421 actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc
      481 aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt
      541 acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc
      601 cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat
      661 gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa
      721 c
//
LOCUS       DAHEYX010000358          718 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.378, whole genome shotgun sequence.
ACCESSION   DAHEYX010000358 DAHEYX010000000
VERSION     DAHEYX010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.378"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(256..456)
                     /locus_tag="MB219_002748"
     CDS             complement(256..456)
                     /locus_tag="MB219_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCC8428390.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          262 a          112 c          125 g          219 t
ORIGIN      
        1 tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg
       61 aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat
      121 tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa
      181 caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct
      241 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
      301 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
      361 atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga
      421 aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg
      481 atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg
      541 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
      601 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
      661 aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttat
//
LOCUS       DAHEYX010000359          715 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.379, whole genome shotgun sequence.
ACCESSION   DAHEYX010000359 DAHEYX010000000
VERSION     DAHEYX010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.379"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            117..>715
                     /locus_tag="MB219_002749"
     CDS             117..>715
                     /locus_tag="MB219_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCC8428391.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILP"
BASE COUNT          290 a           93 c          119 g          213 t
ORIGIN      
        1 aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaattat atcttcttaa
       61 acaatgataa tattaaaatg caagcgcttg taaaaaataa ggagaagagg taaaaaatga
      121 agaaaaaact attaacttta tttcttgtga tgtctttctc aatcgttttg agtgtgtact
      181 acaatactat tgttttttca gaacaaataa actacacaga agaacagtat aatatagaaa
      241 aagaaaaagc tttaaaagtt ggttatagtg aagaacagtt taagcagatt atggaaatac
      301 ctacaaattt atcaaatgaa aatagtgaaa caagaatagt aaattatact atgacttcta
      361 atcaaaccaa agtcattaat aaagcaatgg agcaaattgg aaagccatac gaatggggag
      421 catcgggacc aaattctttt gactgtggtg gtttagtgaa atatgtttac aaacaagctg
      481 taaatattga actgcctatg ggaaccacca atcaagagca atacggtaca gaggtaagtt
      541 taaattcact taagcctggt gatttattat tttacggcaa tcgaggtgca acttatcatg
      601 taggtatata taaaggtaac ggtgtaatga ttcatgcacc gcaacctgga gaaactgtaa
      661 aagaggttaa catacaatat ttttatccta actttgcaaa aagaattttg cctga
//
LOCUS       DAHEYX010000360          707 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.381, whole genome shotgun sequence.
ACCESSION   DAHEYX010000360 DAHEYX010000000
VERSION     DAHEYX010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.381"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            187..>707
                     /locus_tag="MB219_002750"
     CDS             187..>707
                     /locus_tag="MB219_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCC8428392.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPC"
BASE COUNT          277 a           92 c          119 g          219 t
ORIGIN      
        1 atatttattt aaagtagttc ttaatgactt taaaggtagt acataattca ttttatttct
       61 gattttaggt agtatttaat taaaaaacag ttttaatgcg tttgcatttt ttatttatct
      121 ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga aagtagagga
      181 tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga attattttat
      241 caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag
      301 attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat
      361 gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt attaaactgc
      421 caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt attattacaa
      481 aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact atatctagca
      541 gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag agagaatagg
      601 gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga aaaatcagac
      661 gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgcca
//
LOCUS       DAHEYX010000361          690 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.383, whole genome shotgun sequence.
ACCESSION   DAHEYX010000361 DAHEYX010000000
VERSION     DAHEYX010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.383"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..549
                     /locus_tag="MB219_002751"
     CDS             <1..549
                     /locus_tag="MB219_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428393.1"
                     /translation="IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQ
                     KRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSW
                     SKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHD
                     RDINASINILTEGLRLHSMGLA"
BASE COUNT          256 a          111 c          132 g          191 t
ORIGIN      
        1 attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga acaacgcaaa
       61 ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga agctaaaaat
      121 tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa tcaacgtact
      181 gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat ctgtattgaa
      241 gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat ttctgatgtt
      301 tcttggtcaa agtttgtgac taaattacaa tacaaggctg actggtatgg acgaaaaatc
      361 atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg acataaagat
      421 ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac tcatcatgat
      481 cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca ttctatggga
      541 ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg gtaaataaga
      601 gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa gaagctccca
      661 cttcaagcgt taagaaccgt caggtttagc
//
LOCUS       DAHEYX010000362          689 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.384, whole genome shotgun sequence.
ACCESSION   DAHEYX010000362 DAHEYX010000000
VERSION     DAHEYX010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.384"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            222..478
                     /locus_tag="MB219_002752"
                     /pseudo
     CDS             222..478
                     /locus_tag="MB219_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          237 a          105 c          131 g          216 t
ORIGIN      
        1 aggcttcttc aaaagtacat cctccagtaa attctaagta ctggaggatt ctttttctag
       61 tgttgcttta ctcatgaaaa agtagttggc tacttcatca aaatttctga acttttttaa
      121 tgtataataa agagctcgat catttactac aaggatgcta gctcctacat tttcacagtt
      181 ttttagcaag taaatttttc taaaatcaca tcaggaaatg catgagattc tttaagaaag
      241 gcaattatag ggctatgatt acggttcgtg taagtgacgc caaaaaagaa tggctttctt
      301 atatggctga attttatggg attactttat ctgaacttct aaaaaactat tcgatggaac
      361 aactagaaga tgaatacaat cgtcaaacgg ctgaaattgc ccataaacat tggataagaa
      421 gtgggaaaac aacagtattc atggaagaat ccttaatgaa tttggtgggc tggaatgacc
      481 tgccgtttaa aattcacgcc cgaaattcag aagcagctct gattggcaaa tacgtgtatt
      541 ggtagagata gaagataatc aattcgttgt taccgctatt caagtagggc accgaagaaa
      601 tgtctatgat taagagactg tttattttta caaaaaaata ataactggaa tctagttaaa
      661 actcttgaag tgagagattt gactagatt
//
LOCUS       DAHEYX010000363          689 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.385, whole genome shotgun sequence.
ACCESSION   DAHEYX010000363 DAHEYX010000000
VERSION     DAHEYX010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.385"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(4..681)
                     /locus_tag="MB219_002753"
     CDS             complement(4..681)
                     /locus_tag="MB219_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCC8428394.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT          206 a          131 c          120 g          232 t
ORIGIN      
        1 tttttaatta gaaagcaagt gtaaatggct agttcttgaa tggaactgag aatcatttgt
       61 atgtaaatgt tcaccattaa aaacttgata tgagttatct gttgttttac acagctctgt
      121 gattctccaa aggctacttg taccttttac ttctactaaa acaccaaaat cacggtcgca
      181 ggcataattg taaacgactg cagaagggtg ttttgtgatg cccatttccc gagcaatttt
      241 ttgatcagaa aagaatactt ctttaacgaa gtcagactga cgatcttctt taaacatatt
      301 caaatcgccg ccgatttctt caaatatgga ttcagcaagt tcttgagaat atggtatgtc
      361 attacagccg actgcttctt gcaatttgat caaaaaacgg cggccttttt tctttccttg
      421 aaactgcgcc gctttgcagt ccaatgctgc agaataagtc attgagaata agtgattacg
      481 ttcctcaaga ttgttctgag aaattccttt ttgttgcatg acatccccaa ttgtctgcaa
      541 attgaccaaa ggaagaaagc gaaattgcat cttttctttt gatggtgtcc cgtactcttc
      601 ttcgataaat tgtaataact gttgttccat ttcgaaacag tattccccta aaggattaac
      661 aaataaatag atttcgatca tatgaatct
//
LOCUS       DAHEYX010000364          676 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.387, whole genome shotgun sequence.
ACCESSION   DAHEYX010000364 DAHEYX010000000
VERSION     DAHEYX010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.387"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(122..499)
                     /locus_tag="MB219_002754"
     CDS             complement(122..499)
                     /locus_tag="MB219_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428395.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          208 a          113 c          117 g          238 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatg
//
LOCUS       DAHEYX010000365          672 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.388, whole genome shotgun sequence.
ACCESSION   DAHEYX010000365 DAHEYX010000000
VERSION     DAHEYX010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.388"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>672
                     /locus_tag="MB219_002755"
     CDS             <1..>672
                     /locus_tag="MB219_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428396.1"
                     /translation="VSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAEKSSTQKSAST
                     ETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLAGYSDLQIEYA
                     RVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVMLTGKYSYQGVL
                     VYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAIPTGNPNDVKQ
                     LINVIV"
BASE COUNT          219 a          141 c          120 g          192 t
ORIGIN      
        1 gtctctttat tccttgtagg atgtacgtct cctagtaaag aaaatagtga tccatctgcg
       61 accacagaca aaagttcaat caaacaaacc agttcagctg aaaaatcaag tactcagaaa
      121 tcagcttcta ctgaaacgaa tacatcttca acaagcagta aagtctcaga gcccaatact
      181 tcttctacat ctagtagtac agttgagaca aatatttctt cttcaacaat agacacaact
      241 tcctcacagt cttctcagtc aatagatccg ttagcaggat attctgatct acaaattgag
      301 tacgcacgtg tttggctagc aattagaggt aatactttta gagatacatc gggtgatttc
      361 gaactacatg ttattcatta tcctgcagga acacctatcg ccccttatga tgcagggagt
      421 gcagtctatc cgaaagatgt ggttatgctc actgggaaat acagttacca gggagtttta
      481 gtgtattcaa gtaaccatga cgggacaatc actagttatc cagttccttc acattggcaa
      541 atgcctgcag atcaaacatc ttatccagcg ttcattagag aaacaactca agaaattatt
      601 gataatgcat ctgtagtagc tattcctaca ggtaatccaa atgatgtgaa acagctgatc
      661 aatgtgatag tt
//
LOCUS       DAHEYX010000366          649 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.390, whole genome shotgun sequence.
ACCESSION   DAHEYX010000366 DAHEYX010000000
VERSION     DAHEYX010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.390"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>649
                     /locus_tag="MB219_002756"
     CDS             <1..>649
                     /locus_tag="MB219_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCC8428397.1"
                     /translation="IYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFI
                     ILTPQLFMQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISK
                     AQAEGKSENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLIS
                     CLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATI"
BASE COUNT          199 a           92 c          115 g          243 t
ORIGIN      
        1 gatttatgtg ttcattactt caattactaa ctttttagga agtttagttt tctggttgag
       61 tatccacgga aaagtaggta aaccactctt taactctaat gtttttaaac agttgtggaa
      121 accagctttt attattttga ctcctcagtt gtttatgcaa gtgtacacaa ctttagacaa
      181 aacgatagtt gggtattttg tggatccaac agcattatct tattatgatc agtctcaaaa
      241 aattgctcgt attatattgg cagtcttaac atcaattact attgttatgt tgcctaaaat
      301 atctaaagca caggcagagg gaaaatcaga aaatatttta gtttacacaa aaaaatcttt
      361 tgattacacg attattattg cattaattct ttttagtgtg gtgtttatca atacaaaaga
      421 atttgttcca tggttttttg gggagaattt caaaccgatg acaataaaca tgcttatttc
      481 ttgtctaatt atagttgtta gtccaattgg tggagttttt gctaaccaat ttgcaattgc
      541 tatagaaaaa gataaggagt atgcttatcc aatgatcatt ggtgcgattg taagtattat
      601 tggaaatgta gcttttgtgc ctacttatgg cgctgtagcg gctacaatc
//
LOCUS       DAHEYX010000367          634 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.393, whole genome shotgun sequence.
ACCESSION   DAHEYX010000367 DAHEYX010000000
VERSION     DAHEYX010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.393"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            19..234
                     /locus_tag="MB219_002757"
     CDS             19..234
                     /locus_tag="MB219_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428398.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(452..634)
                     /locus_tag="MB219_002758"
                     /pseudo
     CDS             complement(452..634)
                     /locus_tag="MB219_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          213 a           91 c          116 g          214 t
ORIGIN      
        1 aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat aaaaaaaacc
       61 cttaatgatt tcacttccaa ttcaatatca aaaggtactg gtatcaattt aagcacaata
      121 aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga tgctataaaa
      181 ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag ataattatcg
      241 tctagtgaac tacttgccac ttagctgaat ttaacgatcc ttagtgcttg aagtgggaga
      301 ttcttgggaa tagagcatac ttgcaagctt atgtctttat taacagcgat atctcttatt
      361 aaccaagcta tccccgtagt tctaaggttt ttacttttat ctaagctaat gatcgtattc
      421 aaaggaaatt tttgaccctt agaaagtctc attttatcag acatgagctt aggcattgct
      481 gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt agtctttatc
      541 acgatttcac tcaactaagg taatgtgatg tgattctgat attctttcaa agatagtttt
      601 ttgcataagc taacattaca tcatcaatga gatg
//
LOCUS       DAHEYX010000368          630 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.394, whole genome shotgun sequence.
ACCESSION   DAHEYX010000368 DAHEYX010000000
VERSION     DAHEYX010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.394"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(223..588)
                     /locus_tag="MB219_002759"
     CDS             complement(223..588)
                     /locus_tag="MB219_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428399.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          241 a           87 c          114 g          188 t
ORIGIN      
        1 tcaacacttt attctttgaa tattatagat gtttccacct caaattcaat atctattatt
       61 ggaatcatta ttgctgcaat tattacacaa ctgtttttag tgtttggaga gaagaaaaga
      121 agtaaaaaat agaaaaacta gtgatagttt tggtgatata agaaaaacat atatgtataa
      181 aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt
      241 tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc
      301 tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt
      361 aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga
      421 tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt
      481 gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg
      541 aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca
      601 cctaacaagt gttattattt tgttagtttt
//
LOCUS       DAHEYX010000369          622 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.395, whole genome shotgun sequence.
ACCESSION   DAHEYX010000369 DAHEYX010000000
VERSION     DAHEYX010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.395"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..362
                     /locus_tag="MB219_002760"
     CDS             <1..362
                     /locus_tag="MB219_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428400.1"
                     /translation="TIPTFADSTNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTEN
                     YLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYK
                     SAGNNWIATYSGTLTRK"
     gene            372..575
                     /locus_tag="MB219_002761"
     CDS             372..575
                     /locus_tag="MB219_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428401.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          249 a           95 c          100 g          178 t
ORIGIN      
        1 ctacaattcc tacatttgct gatagtacaa atgaaccaac tactgtagta gataataacc
       61 aagaattaac tcctttagat aggaacggaa tagccattgg taacccaact aacgacttaa
      121 ctgttactga aaattattta atagtttctc cattacttgc tcgagcagct tctaaaacga
      181 tacgagtgac aggaacgatg atttttgata aagttggttg gccttctaga cctcctcaat
      241 cacggaattg gaaagaaaaa agaaatggtt attggtacta tggaaaattg tatttacaaa
      301 gttataaatc agctggaaac aattggattg cgacttattc aggaacctta actagaaagt
      361 agggattttt tatgccagaa tttgtgaaca caccaaattt ttatcaagat agcgatattc
      421 taagtaaaat taatttaccg aaagaagaat cagctttaca agttcagcga actttacagg
      481 agacacaaaa aattcaaaaa tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa
      541 aaagagacca aaaaacatat caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt
      601 cgacattctt tttttctaat tg
//
LOCUS       DAHEYX010000370          620 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.396, whole genome shotgun sequence.
ACCESSION   DAHEYX010000370 DAHEYX010000000
VERSION     DAHEYX010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.396"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..181)
                     /locus_tag="MB219_002762"
     CDS             complement(<1..181)
                     /locus_tag="MB219_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCC8428402.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLF"
     gene            complement(312..>620)
                     /locus_tag="MB219_002763"
     CDS             complement(312..>620)
                     /locus_tag="MB219_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCC8428403.1"
                     /translation="FENKRIFDLVLFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTI
                     GMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS
                     "
BASE COUNT          235 a          102 c           80 g          203 t
ORIGIN      
        1 aaaacagaac tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca
       61 atactaaaac tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa
      121 aaataatgac cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca
      181 ctctttcacc tccaaaaaat aagatcgcac tgttatctta agctgataaa aatgcaccgt
      241 tattcagata acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag
      301 ctttttttta attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt
      361 ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta
      421 gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg
      481 atcattccga tagtctttaa cttatctaga caacagactg tcttttttgt cacaaatgat
      541 gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa aaccaaatcg
      601 aatatacgtt tattctcaaa
//
LOCUS       DAHEYX010000371          618 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.397, whole genome shotgun sequence.
ACCESSION   DAHEYX010000371 DAHEYX010000000
VERSION     DAHEYX010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.397"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            42..>618
                     /locus_tag="MB219_002764"
     CDS             42..>618
                     /locus_tag="MB219_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCC8428404.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT          229 a           92 c          122 g          175 t
ORIGIN      
        1 ataagattcc tccataaacg aatttatgga ggaattattt tatgtctgag gagttattgc
       61 gcgctgcacg taatggtgac gaagaagcat ttgctgccct gtatcaaaaa tattttccac
      121 ttttactacg tttgaaaaac acctattatt taagaaatta tgtttctgag gattgggaac
      181 aagaagcaag gatcgcttta tatcaggcat tgagaactta cgaaaaaagc aaaggtgtta
      241 gctttggaag ttattataaa agcattataa gcaatcaaat ctattctctg ttgaggaagc
      301 agaacgcgct gaagcgtcgt gatcaaaaag atgaagtctc aatcgaccaa aaaatagaga
      361 cagaaggacc agacttttta gcagagatca cattgcaaga acctttagcg attcagcatc
      421 ttttattaaa agaagcaatc gaaaattgcg aaatccagtt ttcaaaaata gaagctaaaa
      481 tttttcatta ttatatgcaa ggcgttgatt ttgtagacat ggctgaatgt ttagcaatgg
      541 atcacaagca agtaatgagc gcttacggga gagtaaaact caaaataaaa aaacacttga
      601 aattttataa tgaggaat
//
LOCUS       DAHEYX010000372          617 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.398, whole genome shotgun sequence.
ACCESSION   DAHEYX010000372 DAHEYX010000000
VERSION     DAHEYX010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.398"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..473
                     /locus_tag="MB219_002765"
     CDS             <1..473
                     /locus_tag="MB219_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HCC8428405.1"
                     /translation="NILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDSIATFVPERVL
                     NLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDSYYVNAEQELYTTLLKA
                     YQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYESSPSTITKDWLE"
BASE COUNT          258 a           87 c           89 g          183 t
ORIGIN      
        1 ataatatatt agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc
       61 aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc
      121 ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa
      181 ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtggt atttcgatag
      241 tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt
      301 taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg
      361 tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac
      421 gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta
      481 ctgacaaagt gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct
      541 aattatatta cgaatattac aaaataatag ctattttaat agaatttata agttaaaata
      601 ttatgataaa aatcaca
//
LOCUS       DAHEYX010000373          588 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.400, whole genome shotgun sequence.
ACCESSION   DAHEYX010000373 DAHEYX010000000
VERSION     DAHEYX010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.400"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..445
                     /locus_tag="MB219_002766"
     CDS             <1..445
                     /locus_tag="MB219_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HCC8428406.1"
                     /translation="KLNKYVTFSVNNEDFWSSLDVNYSKGKTERGRVFYISISYNTAD
                     NQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKSVKVTKSGYTVWGDLNFWTKSG
                     ISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYINSEAAVEMN"
BASE COUNT          223 a           68 c          109 g          188 t
ORIGIN      
        1 aaagcttaat aagtatgtta catttagtgt taataatgaa gatttttggt cttctttaga
       61 tgtaaattat tcaaaaggaa aaactgaaag aggccgagta ttttacatta gtatttcata
      121 taataccgca gataaccaac cgatttattc agtatataca gatgaaacat gtacggaatg
      181 gcgagggtat tataagggaa acaactttga agacactcaa attactatgc ttgagaataa
      241 aagcgtgaaa gtaacaaaaa gtgggtatac agtatgggga gacttgaatt tttggactaa
      301 gtcaggaata tcgaacacag gagatatata caccactgac cgaaaatttt ataattttac
      361 caacaatgct tactattatg aattgaaaaa agatgggagg gtctatggct atataaatag
      421 tgaagcagct gtagagatga actaaaatat tgctgtccct taatcttatg gggcagcaat
      481 atttttatat tattaatcat ttttctgaag cgagggtaga agaattaatc aatagttttt
      541 cttgttttgt aatataatag tttaattacc aaaaattatt taagtaaa
//
LOCUS       DAHEYX010000374          583 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.401, whole genome shotgun sequence.
ACCESSION   DAHEYX010000374 DAHEYX010000000
VERSION     DAHEYX010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.401"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
BASE COUNT          201 a          102 c           74 g          206 t
ORIGIN      
        1 acaagtagtc attaagtctt cataagatgt acaatatgac attaaaaaaa taaaagctta
       61 cgaccatccg ttaaatctga cactaaataa tggactgctt tcttttgtat tagcaggtta
      121 cgttgactat gaatattcac cagattattt ggagaaacct cttcaacacg agaacaatga
      181 tattaatttt agatttgtct acgagtcaaa aagtaaaaca tggttagttg atcaattaag
      241 cacgataaac acaagcttcc atgaattacc taaacctgaa aatgaagttg agatggtcat
      301 taaaatcttc taaaaaagaa gcgttgaagg ggaaatattc ctttcaacgc tcttttttat
      361 atgcttagaa acataaataa attgtatact ttctttttag cccctattac ttttcacgaa
      421 aaatatcttt ttttcgacgc tagcgtttct ctacttctaa tactgtacac agtttttgta
      481 ttattgaaaa attactcatt atttagcatt aatccacatc gttttatcaa tcgaccttca
      541 tactaaacta ctttaaagtc atcttccttc tcgtttttca tta
//
LOCUS       DAHEYX010000375          583 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.402, whole genome shotgun sequence.
ACCESSION   DAHEYX010000375 DAHEYX010000000
VERSION     DAHEYX010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.402"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(243..392)
                     /locus_tag="MB219_002767"
     CDS             complement(243..392)
                     /locus_tag="MB219_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428407.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          182 a           92 c           90 g          219 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tac
//
LOCUS       DAHEYX010000376          572 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.403, whole genome shotgun sequence.
ACCESSION   DAHEYX010000376 DAHEYX010000000
VERSION     DAHEYX010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.403"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            17..463
                     /locus_tag="MB219_002768"
     CDS             17..463
                     /locus_tag="MB219_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCC8428408.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          190 a           86 c          116 g          180 t
ORIGIN      
        1 aacgtggggt gtttacatgg aaaataagtt agtcgaagag gttatcgaaa agttaaaaaa
       61 agagaacatt cgaatcacac ctcaacggta cgcggtttta gaatatttga ttgaacatca
      121 ttcccatcca acggctgatg agatttataa agcacttgaa catcggtttc caaatatgag
      181 tgttgcgact gtttacaata accttcgctt atttacttcg attggatttg ttcaagaaat
      241 gaagtacggc gatgcttcta gccgatttga tttcagttca aagcgtcatt atcatgtaat
      301 ttgtgaaaac tgcggtaaga ttgttgattt tcattaccca gggcttgaag atatagaaat
      361 ggctgcaagc agactgactg attttgagat taatgaacat cggttggaat tgtatggact
      421 ctgtccggat tgtaaagagt cagcaaaaaa ggcgcaaatg taaaaacagt ttctacaagt
      481 gttataccta aaaaaagcta gctgtgtaaa aaaaacgcta gcttttttat ttttttgtct
      541 tttattcaca atcttagata taaaagtgaa ta
//
LOCUS       DAHEYX010000377          565 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.404, whole genome shotgun sequence.
ACCESSION   DAHEYX010000377 DAHEYX010000000
VERSION     DAHEYX010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.404"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..448)
                     /locus_tag="MB219_002769"
     CDS             complement(<1..448)
                     /locus_tag="MB219_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428409.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          174 a          101 c           75 g          215 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atata
//
LOCUS       DAHEYX010000378          546 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.406, whole genome shotgun sequence.
ACCESSION   DAHEYX010000378 DAHEYX010000000
VERSION     DAHEYX010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.406"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            131..418
                     /locus_tag="MB219_002770"
     CDS             131..418
                     /locus_tag="MB219_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428410.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          196 a           87 c           99 g          164 t
ORIGIN      
        1 ctttttttgt gaaaagtaca attagttgtc tttcttcgaa ttttttgtca caataagcag
       61 caaagataaa gctgttacat aaaagaacaa gcagctttat ttgattttga aaagaaaggt
      121 gagaaagaag atgaataaag aatattttgt ccaacaagat gcatttttag aagattataa
      181 aagtgcttcg aagaaattaa cgatcaatat tgatgagttg gcagatcaag tactctctca
      241 gttgaatgaa acgcaagagg aatattacac gatgccagca aatgacgcca aagataatgt
      301 ttctcatcgt tttcctttca gtagacgcat ctactcagat gaaaatgacg gcacattgaa
      361 tacagagtac acatatgaag gtcagccata tgtgctagaa actgatgatg atctttaaag
      421 acagtttttg aaaaggccgc tcatccttaa gtgatgacaa aaaatcatca caattaccga
      481 tgactgtctc tttactatct tttaatcaga aaaaggggtt atgacaagct ttgtcaaccc
      541 cttttt
//
LOCUS       DAHEYX010000379          542 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.407, whole genome shotgun sequence.
ACCESSION   DAHEYX010000379 DAHEYX010000000
VERSION     DAHEYX010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.407"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(32..313)
                     /locus_tag="MB219_002771"
                     /pseudo
     CDS             complement(32..313)
                     /locus_tag="MB219_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            424..>542
                     /locus_tag="MB219_002772"
     CDS             424..>542
                     /locus_tag="MB219_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCC8428411.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNR"
BASE COUNT          190 a           96 c           78 g          178 t
ORIGIN      
        1 cacattagct tatttgtcct ataaaaataa attacggtat tttagaaaag ctattttctc
       61 ttttttcaac gatcttcagc aatttttctt tatcgtcagg taactcaagt tcatgaattc
      121 tagcatgaca attgggacat aacgcgatac agttttcaat cacatctttt ccaccttgcg
      181 acaaatattc tatatgatga caatgtaaat aaggtacacc gtcttttata aatggccctt
      241 tttcttggca aagttgacaa ataccttttg caagtcgttt aacatattca gatacgaagg
      301 gattacgttt atacccgtgg tgctagttaa ttttttccta gaggaaaatg tctaaacatg
      361 ttggtaaatc aacaaaaaaa cgcatatagt agtctcaaaa ctcccaagaa atgaggacta
      421 catatgcgtt catcaaaata tactgaacat tatacagata cttttataac attcaaggaa
      481 attatgagaa ctgtttctaa catataccac aattgtgtac cagataagat taaaaaccga
      541 ag
//
LOCUS       DAHEYX010000380          537 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.408, whole genome shotgun sequence.
ACCESSION   DAHEYX010000380 DAHEYX010000000
VERSION     DAHEYX010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.408"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            241..>537
                     /locus_tag="MB219_002773"
     CDS             241..>537
                     /locus_tag="MB219_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428412.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT          201 a           90 c           96 g          150 t
ORIGIN      
        1 agaaacgggg aaaagcgttt attgtctgtt tatatgtgta tttctgcata acagaaagtt
       61 gtctcagaag tcccatcact tcctttatgc tgaactagta ttctcctgct aattccttat
      121 tcacaagtta caatattcat ataacgcgaa ttgtaaaatt aagttagaaa aataaaaagg
      181 catttatggt acactcaaat tgtatttccg acgaaagaaa acaaaggagt gtaaccataa
      241 atgacctaca aacatcttac catagacgaa ctgacaatga tagaatcata ttatcttcaa
      301 cataataagc cggttgaaat cgctaaccga atgggacgtg ctatacaaac tatttataat
      361 gtagtcaata agttcaagca aggcaagact gctcttgatt attggcacca gtataaagaa
      421 aataagaaaa aatgtggtag aaaagtcatt caattacctg ctcatgaagt agattacatt
      481 aaagagaaag tcactcttgg ttggacgcct gacgtcatta tcgggcgaaa agaaagg
//
LOCUS       DAHEYX010000381          534 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.409, whole genome shotgun sequence.
ACCESSION   DAHEYX010000381 DAHEYX010000000
VERSION     DAHEYX010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.409"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>534
                     /locus_tag="MB219_002774"
     CDS             <1..>534
                     /locus_tag="MB219_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HCC8428413.1"
                     /translation="SNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGDIDQAI
                     YGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNK
                     NDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKE
                     LPYCNFDAINISPIKVN"
BASE COUNT          201 a           77 c           94 g          162 t
ORIGIN      
        1 ctagtaatat aagggcgatt tatgtggatg aatttcaaga tactagagaa ttacaataca
       61 atgtaatagc taaattactt caaaacaacc ctcaaattca gtcaatgttt gttggagata
      121 ttgaccaagc aatttatgga tcattagatg gtatagctaa atcagttgaa gaactagaaa
      181 tgctaactag tcacactttt caatcaaaga cgctacatgg ctgttacagg tctaatcaga
      241 gattagtaga cttctactct aattttcaga gttgtcctta cgagattgaa agcagaggga
      301 ataataaaaa cgaccgtggt tttataagtt atgattgcaa agttactaag gaggatttac
      361 ccaacataat aaaaaatata ataaaggaaa agattgaaag cggcattcat gaaaaagata
      421 tttgtgtact tgctcctcaa cactatcctc tgttttcact gggtgagtat ttaaagaaag
      481 aattaccgta ttgcaatttt gatgctataa atatatcacc aattaaggta aata
//
LOCUS       DAHEYX010000382          531 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.410, whole genome shotgun sequence.
ACCESSION   DAHEYX010000382 DAHEYX010000000
VERSION     DAHEYX010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.410"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            268..>531
                     /locus_tag="MB219_002775"
     CDS             268..>531
                     /locus_tag="MB219_002775"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCC8428414.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          180 a           99 c           80 g          172 t
ORIGIN      
        1 gtattaaaaa tatatacaaa aagtattatt tcgagaaaga taaatatttt caaaaacctt
       61 ttcataatcc tcaccctttc ttacactgat aaatcatgcg tctaatattc aaattatact
      121 cgatatgctt tagctaatca gccaactctg tttgtctact atcattatat caatagacaa
      181 tatctgctcc cgtctttatg atcgtgccaa agcaatcaaa agaaaattgc gaatcactta
      241 gatcgaaagt ttactcaaac aaaagcattg tcagcggttg tcacggattt aacttatgtt
      301 cgggtcggga caaattgggc ttatatttgt ttgatgattg atttattcaa tcgtgaaatt
      361 attggccatt cttgcggaaa taaaaaagat gcgcagcttg taaaaagggc cattcaaagc
      421 attccttact cacttcaaga aatcgaactc ttccatacgg atcgtggaaa agaatttgat
      481 aaccaaacca ttcagaatct catgaatggt ttggttatca aattcctttc c
//
LOCUS       DAHEYX010000383          527 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.411, whole genome shotgun sequence.
ACCESSION   DAHEYX010000383 DAHEYX010000000
VERSION     DAHEYX010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.411"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            47..400
                     /locus_tag="MB219_002776"
     CDS             47..400
                     /locus_tag="MB219_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428415.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
BASE COUNT          176 a           81 c           87 g          183 t
ORIGIN      
        1 atttatcagc gagtggtccg ctgaaataat ttaaggtggt aaaaaaatgg caaaaattga
       61 tgattaccgt tcagacatga ttgaatatat tgaactcttc gctaaaaatt ctaacaataa
      121 tattattatt caaactggag gagcaacaat ttgcggaact cctatagact ttgatgctga
      181 agtaaaagta aatcctctta ttgatgctat gatggattct tttgcagaat ttcgctcaaa
      241 aaaaattgat gatatagaaa aagatgatga gcaaagtttg gtagtcaaat ctatctttct
      301 gaaagatgtt accatcatag gcgagagaac tactaatatc ccatttttag ttgtttttgc
      361 tgatcagatt tctgcaattt ctctcgggaa cttggagtaa caatagattc atttaaatta
      421 tctatattga ttaaattgct aatctctcgg ctattacaaa tagctgagag gatttctttt
      481 atttctcctg aagtaccttc aatcgataat ttcatattct taccctc
//
LOCUS       DAHEYX010000384          523 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.414, whole genome shotgun sequence.
ACCESSION   DAHEYX010000384 DAHEYX010000000
VERSION     DAHEYX010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.414"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..332
                     /locus_tag="MB219_002777"
     CDS             <1..332
                     /locus_tag="MB219_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428416.1"
                     /translation="SEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(386..>523)
                     /locus_tag="MB219_002778"
     CDS             complement(386..>523)
                     /locus_tag="MB219_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCC8428417.1"
                     /translation="EFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCL
                     A"
BASE COUNT          178 a           81 c          105 g          159 t
ORIGIN      
        1 tttctgaacc agtaaagatc atcatcgatt tttcttatgg tgctgcctat aatcaattta
       61 tcaagaaaaa tttaagcttg tacattaatc tgaacacgga agtgatagag gcaggaaagc
      121 ctgagttccc ggatgttata atcacgaata ggaataatct atatgaagaa agtgcatctc
      181 aagtagtggt ttggctcgat ccgccaagag cagtagactg ggggaatctt acaaagattt
      241 tgttgactat ccaagaagag aaatacaaag acagcaaaat tgacaagcaa atagacgatt
      301 ttcctgaaga aatcatcgac aggatcgaat agtgaacttc ttttagaaag tccgactgct
      361 ttttctaaaa atattatttg tcaaattaag cgagacaaaa ctttccattt acgtaactca
      421 aaaaaacttc taatggtgtt tggtaattta gtgattttct agggatatgg tttcttttgg
      481 acgcaacgga tgagatgaat ccttgattga cttggttgaa ttc
//
LOCUS       DAHEYX010000385          522 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.415, whole genome shotgun sequence.
ACCESSION   DAHEYX010000385 DAHEYX010000000
VERSION     DAHEYX010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.415"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            417..>522
                     /locus_tag="MB219_002779"
     rRNA            417..>522
                     /locus_tag="MB219_002779"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          198 a           91 c          101 g          132 t
ORIGIN      
        1 tataaaatag ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa
       61 ggaccgcttg cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac
      121 aaacgacaag ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac
      181 ttttaaaaaa gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca
      241 gccaaaaacg aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta
      301 agacaaacca aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa
      361 gcaagcaata tgctagcaaa caaattgagc ttaacaatcg caaggttgtt cgaacttttt
      421 atgagagttt gatcctggct caggacgaac gctggcggcg tgcctaatac atgcaagtcg
      481 aacgcttctt tttccaccgg agcttgctcc accggaaaaa ga
//
LOCUS       DAHEYX010000386          516 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.417, whole genome shotgun sequence.
ACCESSION   DAHEYX010000386 DAHEYX010000000
VERSION     DAHEYX010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.417"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(227..>516)
                     /locus_tag="MB219_002780"
     CDS             complement(227..>516)
                     /locus_tag="MB219_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428418.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          180 a           81 c           76 g          179 t
ORIGIN      
        1 atttgaagct attaaaaggc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca
       61 attggagtgg tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat
      121 atagatatca tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag
      181 tatggtgata aaattcgttc cgggctacct ttttaggtag cctactttaa tctttgtatc
      241 tttctggatc aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt
      301 ccggcacgtc agtcacgata tcaaacaaaa agtatacatc cttcttcatt ctagttttcg
      361 cagcaggaat tttgaaatag ttcttattag aatagtagag attgattaat aagctatctt
      421 cgattgctaa aaagaaaact tctgaattcc atactttata aaaatctttg ataaatctat
      481 tcgaagggtc aaatttaaac cataattgtg tctcat
//
LOCUS       DAHEYX010000387          503 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.419, whole genome shotgun sequence.
ACCESSION   DAHEYX010000387 DAHEYX010000000
VERSION     DAHEYX010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.419"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            15..>503
                     /locus_tag="MB219_002781"
     CDS             15..>503
                     /locus_tag="MB219_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCC8428419.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          183 a           91 c           84 g          145 t
ORIGIN      
        1 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
       61 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
      121 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
      181 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
      241 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
      301 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
      361 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
      421 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
      481 agtatgtcgc tatttttatg gac
//
LOCUS       DAHEYX010000388          502 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.420, whole genome shotgun sequence.
ACCESSION   DAHEYX010000388 DAHEYX010000000
VERSION     DAHEYX010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.420"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(9..359)
                     /locus_tag="MB219_002782"
     CDS             complement(9..359)
                     /locus_tag="MB219_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCC8428420.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
BASE COUNT          177 a           97 c           59 g          169 t
ORIGIN      
        1 ttcatgaatt atttttttac taaatgcgtt atagggtatg gtttaccaaa accatctaag
       61 tcttcttcag attcaatttc aaatccatgg cttaaataaa aatgttttgc atatttattt
      121 tgactattaa catcaattct ttttatatct tcagtttcaa tcagctcaat taatatttga
      181 ctaccatatc gcttaccaat aaaatctgga tcaagaaata gcataactaa ttcttctgca
      241 cttacaccac taaaaccgac aatttctccg ttatcttccc ataaataaag tttaacctca
      301 tctaagttct caggaatgat tgctttataa aacgccgttt gtataattaa gcgagacaaa
      361 taaaaaagcc atttatgtta cactcaaatt gtaatacccg ctaaagcata caaggagagt
      421 gaacataaat gacttatacc catcttacac caaacgagct tgtaatgata gaagcatatt
      481 ttcatcaaga aactccggtt gc
//
LOCUS       DAHEYX010000389          493 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.421, whole genome shotgun sequence.
ACCESSION   DAHEYX010000389 DAHEYX010000000
VERSION     DAHEYX010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.421"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..168)
                     /locus_tag="MB219_002783"
     CDS             complement(<1..168)
                     /locus_tag="MB219_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCC8428421.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSIS"
     gene            complement(187..348)
                     /locus_tag="MB219_002784"
     CDS             complement(187..348)
                     /locus_tag="MB219_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCC8428422.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          170 a           95 c           57 g          171 t
ORIGIN      
        1 tgatattgaa gaattagaac tttcatataa agccttttct atttcattgc ttgatttcat
       61 tattgttaaa caagaataca agcaagaaag aactatagtt aatatgataa aaattattaa
      121 agttctattt ttctttcttg taatatatgc tatagcattt tttatcactt tcctaactac
      181 tcctctctat tttcttactc cataactaat tgcatccact ggacaagcat ttttacatcg
      241 tccacatctg atacactcca gatgattgca attctcaact gaatctttat tcatttggca
      301 aactttttta cattttccac aattaataca cttatcctta tcctgaatcg gctacactag
      361 actagacaga aaaaataagg tgtgtagaat aataaaaaac acactggagg atttttcatg
      421 ccaagacgtc aacgaagaac ctattcaaaa gaattcaaac aacaaatcgt cgatctctat
      481 ctcgctggta agc
//
LOCUS       DAHEYX010000390          487 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.423, whole genome shotgun sequence.
ACCESSION   DAHEYX010000390 DAHEYX010000000
VERSION     DAHEYX010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.423"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            108..302
                     /locus_tag="MB219_002785"
     CDS             108..302
                     /locus_tag="MB219_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428423.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          206 a           63 c           82 g          136 t
ORIGIN      
        1 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
       61 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      121 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      181 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      241 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      301 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      361 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      421 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      481 aatcaaa
//
LOCUS       DAHEYX010000391          472 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.424, whole genome shotgun sequence.
ACCESSION   DAHEYX010000391 DAHEYX010000000
VERSION     DAHEYX010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.424"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..76
                     /locus_tag="MB219_002786"
     CDS             <1..76
                     /locus_tag="MB219_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCC8428424.1"
                     /translation="HYSKYLTILKEPKNDTDNHDEPIH"
     gene            42..>472
                     /locus_tag="MB219_002787"
     CDS             42..>472
                     /locus_tag="MB219_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HCC8428425.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNP"
BASE COUNT          160 a           81 c           87 g          144 t
ORIGIN      
        1 tcattattca aaatacttaa caatattaaa ggagcccaaa aatgatactg acaatcacga
       61 tgaacccatc cattgattcc atttatttta caaaacactt tgaattaggt acgatgacac
      121 gctttgattc tccggtacgc tatgtaggcg gcaagggaat caattgcgga cgagcttctg
      181 ctctactagg ttcatcagtt attttaacag ggtttttggg cggttatttt ggcagtatga
      241 ttcaagatat gctgaaaaat gaaaatttgt ttgatttgca ttttttacct attgcagaag
      301 aatcaagagg tgccgtcacg atcatgcatg atgatgaaac gcagacagag ctagtggaag
      361 ctggtcctga aatagcagat actactatca aaaaattaat gaatgatatc aaactaatca
      421 gtaaaaataa ttacctttca gtcattacaa taaatggttc tgttaacaat cc
//
LOCUS       DAHEYX010000392          467 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.427, whole genome shotgun sequence.
ACCESSION   DAHEYX010000392 DAHEYX010000000
VERSION     DAHEYX010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.427"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(1..306)
                     /locus_tag="MB219_002788"
                     /pseudo
     CDS             complement(1..306)
                     /locus_tag="MB219_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
BASE COUNT          156 a          108 c           49 g          154 t
ORIGIN      
        1 gatcctagct gttatcagtt caatcacttc ttccgcaaaa tccataaatt cgattggaat
       61 ctgcgagact atctcactga atcgtttgat cttttcttta gaatctaata caaataattt
      121 gtcagcatcc tcaacatcaa atggcttact cactttagca gaaaagccaa tccctttccc
      181 catcgcaatt tattctattc catcgtcatc gatcaataca gcattatgat tcagcttttt
      241 catcacatac accgttaatt cacctccact atatgataaa acaaaacaac ttgcagactc
      301 cttcatacat atttttgtct ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa
      361 ccctttctta ggtcgtgcct aattgaatag tagcacccta cataatttac aaaaatagtt
      421 taacacataa taaaagcgtt atcaatatct tttttactcg ttttatg
//
LOCUS       DAHEYX010000393          450 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.429, whole genome shotgun sequence.
ACCESSION   DAHEYX010000393 DAHEYX010000000
VERSION     DAHEYX010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.429"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(73..270)
                     /locus_tag="MB219_002789"
     CDS             complement(73..270)
                     /locus_tag="MB219_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCC8428426.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          144 a           83 c           67 g          156 t
ORIGIN      
        1 attacggtga ggaatttttt ctaccgacat aaacaaaaaa accacctcat atgaggcggt
       61 taacgttcaa cattatttta cgatatttac tgcttgagga ccacgttgtc cttcttcaat
      121 atcataagta actgcttggc cttcatctaa agacttaaag ccttctcctt ggattgctga
      181 gaaatgagcg aatacgtcat ttccatcttc accagtgatg aaaccaaaac ctttatctga
      241 gttaaaccat tttactgtac cgttattcat atatatttcc tcctgatacg tatataatac
      301 gtgttttatt gcaattaata ttacttggta aaaggagttt ttatagtgtg aatatatcgc
      361 tcagattacg tttcaaatta gattacttat ttactataac acggatgtaa tgctaacaca
      421 aggataatca ctcgatatac ggtttaaata
//
LOCUS       DAHEYX010000394          450 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.430, whole genome shotgun sequence.
ACCESSION   DAHEYX010000394 DAHEYX010000000
VERSION     DAHEYX010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.430"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..191)
                     /locus_tag="MB219_002790"
     CDS             complement(<1..191)
                     /locus_tag="MB219_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428427.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(291..377)
                     /locus_tag="MB219_002791"
                     /pseudo
     CDS             complement(291..377)
                     /locus_tag="MB219_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          150 a           87 c           75 g          138 t
ORIGIN      
        1 cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt gattttgttt
       61 ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg cttgattgca
      121 aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa acgtaaaaaa
      181 aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc tctttgttac
      241 tcataattgt accataatta gtcacgaatg caaccaatag aaaaaactac ttagaacggt
      301 agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc attaaataca
      361 ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata aaagaagata
      421 tatatcattt cttaattacc tactacctta
//
LOCUS       DAHEYX010000395          423 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.434, whole genome shotgun sequence.
ACCESSION   DAHEYX010000395 DAHEYX010000000
VERSION     DAHEYX010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.434"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
BASE COUNT          137 a           77 c           88 g          121 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa
      241 atcaggtgca tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc
      301 agtgggagca tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta
      361 cactcttagt aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac
      421 ttt
//
LOCUS       DAHEYX010000396          415 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.437, whole genome shotgun sequence.
ACCESSION   DAHEYX010000396 DAHEYX010000000
VERSION     DAHEYX010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.437"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..339)
                     /locus_tag="MB219_002792"
     CDS             complement(<1..339)
                     /locus_tag="MB219_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428428.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAHEYX010000397          405 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.439, whole genome shotgun sequence.
ACCESSION   DAHEYX010000397 DAHEYX010000000
VERSION     DAHEYX010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.439"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..382
                     /locus_tag="MB219_002793"
     CDS             <1..382
                     /locus_tag="MB219_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428429.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           72 c           63 g          113 t
ORIGIN      
        1 aaaagaaatc tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa
       61 taccttctta tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc
      121 actcatagaa aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac
      181 catttactca accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag
      241 aaaagagcag tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac
      301 atacaatcaa aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt
      361 agtttcaatg tttactgagt aactaattat tttgcaggag gacaa
//
LOCUS       DAHEYX010000398          402 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.441, whole genome shotgun sequence.
ACCESSION   DAHEYX010000398 DAHEYX010000000
VERSION     DAHEYX010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.441"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            129..>402
                     /locus_tag="MB219_002794"
     CDS             129..>402
                     /locus_tag="MB219_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428430.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLF"
BASE COUNT          110 a           57 c           56 g          179 t
ORIGIN      
        1 acaagtttca aaatataagt aaaataatat tgttattctt atttctttta cttgtgtcat
       61 aactattttg ttactttaat ttttatttgc ttattttaga aaaaatgtta ttcttttatt
      121 tattctcaat gttaggagtg attattttgc catttattgc gattttattc gatttagttg
      181 cggcagcagc ttattttctc caattaaatt atcaaacctc aggaattctg tttattggaa
      241 tgatttttca aggtgtgatt acccttttgc ttcttgtcat gatgctcagc tataaaggaa
      301 aaagatatgc ccgtattcaa acaaaagtat ttgttaaata tgtttccatt cgctatggga
      361 tcatcattct ttccttttta gtgaatgcga tcgtcctctt tt
//
LOCUS       DAHEYX010000399          402 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.442, whole genome shotgun sequence.
ACCESSION   DAHEYX010000399 DAHEYX010000000
VERSION     DAHEYX010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.442"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>402
                     /locus_tag="MB219_002795"
     CDS             <1..>402
                     /locus_tag="MB219_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCC8428431.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          140 a           77 c           65 g          120 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa tccatgaaaa gcttgccctt aa
//
LOCUS       DAHEYX010000400          401 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.443, whole genome shotgun sequence.
ACCESSION   DAHEYX010000400 DAHEYX010000000
VERSION     DAHEYX010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.443"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            123..341
                     /locus_tag="MB219_002796"
                     /pseudo
     CDS             123..341
                     /locus_tag="MB219_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          135 a           58 c           71 g          137 t
ORIGIN      
        1 atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga gttactttca
       61 atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt gatgaaattg
      121 ccattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat tggtctaatg
      181 gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa aataaagtta
      241 ttccagtatt gctatatgga acacataaca atttttatga attagccgag aacactcgca
      301 actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta tatcaatcaa
      361 ccgaccgaca tatgttaaat ctatatataa taaaaatacc t
//
LOCUS       DAHEYX010000401          401 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.444, whole genome shotgun sequence.
ACCESSION   DAHEYX010000401 DAHEYX010000000
VERSION     DAHEYX010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.444"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>401
                     /locus_tag="MB219_002797"
     CDS             <1..>401
                     /locus_tag="MB219_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCC8428432.1"
                     /translation="KTIKYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKD
                     SESITYLLSGNRYSSEKYVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVL
                     FAPTFRNNIEDNGITQLEWLGVDNLREFFKK"
BASE COUNT          156 a           56 c           70 g          119 t
ORIGIN      
        1 gaaaacgatt aagtatgcaa aaaggaaaaa acaattttat tttcaaacat ggcacggtac
       61 gcctttaaaa aaaattgaat ttgatgcaaa agataagtta cctaaacgtt acctaagcta
      121 tgcgaaaaaa gatagtgagt caattacata tttattatct ggtaatcgct atagttctga
      181 gaagtatgtt tcagcattca atatcgttcc ttctaaaata ttagaagtag gaactcctag
      241 aaacgatgaa ttagcctcta gtaaagagca agtgtttgac ttgaaaaaga aacaaatcaa
      301 tgtcttattt gctccaactt ttcgaaataa tattgaggac aacggaatta cgcaactaga
      361 atggttaggt gtggataact tacgtgaatt ttttaaaaag c
//
LOCUS       DAHEYX010000402          382 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.447, whole genome shotgun sequence.
ACCESSION   DAHEYX010000402 DAHEYX010000000
VERSION     DAHEYX010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.447"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
BASE COUNT          128 a           68 c           52 g          134 t
ORIGIN      
        1 ttacttagaa tatcactatc ttgataaaaa atggtgtatt cacaaattct ggcataaaaa
       61 atacctactt tctagttaag gttcctgaat aagtcgcaat ccaattattt ccagctgatt
      121 tataactttt taaatataat tttccataat accaataacc attacttttt tctgtccaat
      181 ttcgttattg agaagatcta gaaggccagc caactttatc aaaaatcatc attcctgtca
      241 ctcgtatcgt tttagaagct gctcgagtaa gtaatggaga aactgttaaa taattttaag
      301 taacagttaa gtcgttagtt gggttatcaa tggctattcc gtccttatct gaacctcgaa
      361 acccaatcat agaaaaagtt tt
//
LOCUS       DAHEYX010000403          374 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.448, whole genome shotgun sequence.
ACCESSION   DAHEYX010000403 DAHEYX010000000
VERSION     DAHEYX010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.448"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>374)
                     /locus_tag="MB219_002798"
     CDS             complement(<1..>374)
                     /locus_tag="MB219_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCC8428433.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSR"
BASE COUNT          120 a           73 c           68 g          113 t
ORIGIN      
        1 atccgactat ttagacgacg ggtctggcaa acaggttcgc cagtggtaac ctgatatcct
       61 tttagctctg ctaaacaaac actaagccca tttgtaaaaa aagttaaatc attgcgataa
      121 tcttgaatac atcgagcagg aatttctcca ataataatga cctcattatt tttcagttga
      181 gtatttacga tatttgcaca atatttggga gcatcgttat atgcccgtga aagatattcc
      241 tgtggtgcat aaactttaaa actaagatat ggctctaaca attctgttcc agcttttcta
      301 aaggcttgct ccagtacaat aggagtaagc atccgaaaat ctgctggagt actaacaggg
      361 ctatagtata aacc
//
LOCUS       DAHEYX010000404          369 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.449, whole genome shotgun sequence.
ACCESSION   DAHEYX010000404 DAHEYX010000000
VERSION     DAHEYX010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.449"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            124..>369
                     /locus_tag="MB219_002799"
     CDS             124..>369
                     /locus_tag="MB219_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCC8428434.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRN"
BASE COUNT          125 a           74 c           72 g           98 t
ORIGIN      
        1 attttatata ataaaatgca atacttttcg agagtgtaaa atattttgtg taaatagaaa
       61 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc
      121 catatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
      181 gaattattcc gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg
      241 gctttcctcg attacgaaaa gtacgatcgc attggtttta acacggggaa ttctcgtaac
      301 ggctcctatg accgtacggt caagaccgag tacggggaac ttcatctcca gattccgcgc
      361 gaccgcaac
//
LOCUS       DAHEYX010000405          368 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.450, whole genome shotgun sequence.
ACCESSION   DAHEYX010000405 DAHEYX010000000
VERSION     DAHEYX010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.450"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(19..312)
                     /locus_tag="MB219_002800"
     CDS             complement(19..312)
                     /locus_tag="MB219_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCC8428435.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT          107 a           60 c           62 g          139 t
ORIGIN      
        1 ttttcgaaaa tagctattct agtctatctg tttctttact tttcgctgct gatttgaatt
       61 gaattgatca tattcatttt ctagtattaa taacttttcg tatacatcgg tcatattcat
      121 actagaagca agacttagat catatacaaa tgaagcaggt aatgatttta ttctacgttg
      181 tcgggttatc cagcttgcaa ccgttccttg tgggtaggca tataactcac aaaattgttc
      241 tacagtgatt cctaaattct tgataatgta aacattgatc gggtgcgagt aaacaaatgt
      301 attgatagac atggtgcgta agcctacttt cttttattat tgcgtttgca ataatagttt
      361 aacacacg
//
LOCUS       DAHEYX010000406          363 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.453, whole genome shotgun sequence.
ACCESSION   DAHEYX010000406 DAHEYX010000000
VERSION     DAHEYX010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.453"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
BASE COUNT          135 a           46 c           61 g          121 t
ORIGIN      
        1 gataccatgt aacccaaaat agtttattga gaaaatagga gagtatttga tactaactag
       61 taggaaaaaa tgagataaaa agtaaatgga atcatttaag gtaatctgaa ataaaaaata
      121 tacagccctg tggtacttta cctacaaggc tgtatatatt ttttactctc agcgtttttg
      181 actccgtgtc tgactccgtt ttattgattt tgaatgttat tcaatgacca tttaacaggt
      241 caaatttgat gtttttgatt ccgtatgacg ccgtttgata tctagtgata tagtgatttg
      301 agtctacatc gaataaggga ataaaccaat aaaaaatgat ttagaatatt aaaataacaa
      361 aaa
//
LOCUS       DAHEYX010000407          356 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.454, whole genome shotgun sequence.
ACCESSION   DAHEYX010000407 DAHEYX010000000
VERSION     DAHEYX010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.454"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            178..>356
                     /locus_tag="MB219_002801"
     CDS             178..>356
                     /locus_tag="MB219_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428436.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTG"
BASE COUNT          121 a           58 c           67 g          110 t
ORIGIN      
        1 tatagtgcgc ggctacttct gccatgattg caaaaacaaa atcaaaaaga attttcaaac
       61 ctataacctc ctccccttgc cagattaggc cggggtagca gacagcaaga ctattatagc
      121 ataattgaaa tatccaaatg attggatttt tgctataatt ttagcgaggt gattgtgatg
      181 aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacgcaacc gttattggtt
      241 cgctttactt tacaaacgat tcatgaatcg attaattgtg tagttgcaga tattgaaatt
      301 atcgataaat tgctaattat ggatgatgga aaatacaata ttgccgtcac aggtca
//
LOCUS       DAHEYX010000408          354 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.456, whole genome shotgun sequence.
ACCESSION   DAHEYX010000408 DAHEYX010000000
VERSION     DAHEYX010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.456"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            109..312
                     /locus_tag="MB219_002802"
     CDS             109..312
                     /locus_tag="MB219_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCC8428437.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT          153 a           55 c           73 g           73 t
ORIGIN      
        1 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
       61 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
      121 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
      181 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
      241 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
      301 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccg
//
LOCUS       DAHEYX010000409          338 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.458, whole genome shotgun sequence.
ACCESSION   DAHEYX010000409 DAHEYX010000000
VERSION     DAHEYX010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.458"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(57..173)
                     /locus_tag="MB219_002803"
     CDS             complement(57..173)
                     /locus_tag="MB219_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCC8428438.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT          127 a           64 c           42 g          105 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aaacccaaaa gggcataaaa atcccagtga taaatacttt tatcactggg atttttatgc
      121 ataaccatag gtatacaaag catacagata ttctctggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tctaattatt
      241 tgttcccgct atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gtcacttaaa taatagcacg taagagca
//
LOCUS       DAHEYX010000410          333 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.460, whole genome shotgun sequence.
ACCESSION   DAHEYX010000410 DAHEYX010000000
VERSION     DAHEYX010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.460"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(<1..>333)
                     /locus_tag="MB219_002804"
     CDS             complement(<1..>333)
                     /locus_tag="MB219_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428439.1"
                     /translation="ELAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLL
                     RQDPVFKAVLDRKELASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELII
                     DLDSTHSDT"
BASE COUNT          101 a           56 c           72 g          104 t
ORIGIN      
        1 tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc gtatcattac ggatgaggcg
       61 tgctttatcg ataagttctt ggttcaaagc ttgaagttca tggatattct cctcagatag
      121 tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt tctttcctat ctaaaacagc
      181 tttaaacaca ggatcttgtc tcaggagatt agctgcagaa tcagctgagt aaccagcaat
      241 taattgcata atgaactgct caagtatgga taagttgtca tgagtaaaat acgctcgttc
      301 gtcttcaatg tgaatgtgtt gcttagccag ttc
//
LOCUS       DAHEYX010000411          332 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.461, whole genome shotgun sequence.
ACCESSION   DAHEYX010000411 DAHEYX010000000
VERSION     DAHEYX010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.461"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>332
                     /locus_tag="MB219_002805"
     CDS             <1..>332
                     /locus_tag="MB219_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428440.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAHEYX010000412          330 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.462, whole genome shotgun sequence.
ACCESSION   DAHEYX010000412 DAHEYX010000000
VERSION     DAHEYX010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.462"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            245..318
                     /locus_tag="MB219_002806"
     tRNA            245..318
                     /locus_tag="MB219_002806"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:279..281,aa:Ile,seq:cat)
BASE COUNT           97 a           72 c           54 g          107 t
ORIGIN      
        1 tttttgaatg caaaatcaat gcaaaaaatt ttgtatttct ctacttttct ctccttgacc
       61 aaaataaaaa aacgttgatt taacaacgtt tttcatacat gtttatctgt ctcaaccata
      121 catatatgga gacgcatatc ttcactatca ctcataaata gtcacactcc aagatatgcg
      181 ataacaggtt tgttgccacg cattctactg gttgattgtt tatggcttta tgtggcaaca
      241 accactaccc ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt
      301 cgagtcctac agggtacatt aatgtagcca
//
LOCUS       DAHEYX010000413          322 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.463, whole genome shotgun sequence.
ACCESSION   DAHEYX010000413 DAHEYX010000000
VERSION     DAHEYX010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.463"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            complement(44..>322)
                     /locus_tag="MB219_002807"
     CDS             complement(44..>322)
                     /locus_tag="MB219_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCC8428441.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DAHEYX010000414          322 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.464, whole genome shotgun sequence.
ACCESSION   DAHEYX010000414 DAHEYX010000000
VERSION     DAHEYX010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.464"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            138..>322
                     /locus_tag="MB219_002808"
     CDS             138..>322
                     /locus_tag="MB219_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCC8428442.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINV"
BASE COUNT          111 a           58 c           49 g          104 t
ORIGIN      
        1 ttgaaaataa acatcagcag tcctatcaat gcatgtgagc ttatagaagt tattcgcagc
       61 gcagcataat tgcattagct tcttttcgtg ttttttgcct cttttccctt taaatcttaa
      121 tggaggaggt gtgcaatatg tcagataacg acgaacgcgt aaatgcccag ttgattcgat
      181 tctttgaaaa ggtcattttg aacaccgcta atacttatta taaaaaacaa gaagaaattc
      241 aaaaacatga acaacttgat caactcccac catatttgtt tactaaagat actataaaat
      301 ttaaacaacc gattaatgtt tt
//
LOCUS       DAHEYX010000415          297 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.473, whole genome shotgun sequence.
ACCESSION   DAHEYX010000415 DAHEYX010000000
VERSION     DAHEYX010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.473"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
BASE COUNT          125 a           45 c           41 g           86 t
ORIGIN      
        1 gatatttctc ctttattaaa tctgaagtac ttatattaaa ataattctaa actatttacc
       61 tcaaaaagtg caataaatta tttctgaaca ataaaatgat agtcaaaaca aaaaaatatt
      121 gttaatcgtt cagagccatc cgagagtgta aaatattttg tgtaaatgaa aaaatccata
      181 caaaaaagga aggcgcttct gtagaataaa gttaacgaca accaattcac agaaaagagg
      241 acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcg
//
LOCUS       DAHEYX010000416          290 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.denovo.475, whole genome shotgun sequence.
ACCESSION   DAHEYX010000416 DAHEYX010000000
VERSION     DAHEYX010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.denovo.475"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..>290
                     /locus_tag="MB219_002809"
     CDS             <1..>290
                     /locus_tag="MB219_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HCC8428443.1"
                     /translation="IKTIEEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIK
                     SVDTIMYGKSLVALDKENELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNI"
BASE COUNT          109 a           31 c           59 g           91 t
ORIGIN      
        1 ctataaaaac aattgaggaa ggtttgaaag ggttaaataa aaatgatttt cgaaaagagt
       61 ttgttgcttt ttgggacaaa caagagaatc aaatttcgtt attctacctt tgtgaaccag
      121 cgttgtttat caagtcagtt gatacaataa tgtacggtaa gtctttggtt gctttggata
      181 aagagaacga attacaatta aatgcagcga gtagattgtt taatggagag aaagaagata
      241 ctcatgaatt agatgcatac tatgttccaa ttagaaacaa gaataatatt
//
LOCUS       DAHEYX010000417        16073 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.049, whole genome shotgun sequence.
ACCESSION   DAHEYX010000417 DAHEYX010000000
VERSION     DAHEYX010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.049"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..322
                     /locus_tag="MB219_002810"
     CDS             <1..322
                     /locus_tag="MB219_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCC8428444.1"
                     /translation="NCPVCKTNLTECSGPEPQEPQPEEPEAPEEEPKGGAGGLIVFLL
                     VALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPETEFDPEADFVSEQ
                     EDEE"
     gene            319..2400
                     /locus_tag="MB219_002811"
     CDS             319..2400
                     /locus_tag="MB219_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCC8428445.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            2549..6469
                     /locus_tag="MB219_002812"
     CDS             2549..6469
                     /locus_tag="MB219_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCC8428446.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            6470..7414
                     /locus_tag="MB219_002813"
     CDS             6470..7414
                     /locus_tag="MB219_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428447.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRNQLSDSKQAASPKKAAARTKKNELEV"
     gene            7418..7621
                     /locus_tag="MB219_002814"
     CDS             7418..7621
                     /locus_tag="MB219_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCC8428448.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(7921..8157)
                     /locus_tag="MB219_002815"
     CDS             complement(7921..8157)
                     /locus_tag="MB219_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428449.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(8340..8726)
                     /locus_tag="MB219_002816"
     CDS             complement(8340..8726)
                     /locus_tag="MB219_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCC8428450.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(8708..10036)
                     /locus_tag="MB219_002817"
     CDS             complement(8708..10036)
                     /locus_tag="MB219_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCC8428451.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(9997..10326)
                     /gene="mobC"
                     /locus_tag="MB219_002818"
     CDS             complement(9997..10326)
                     /gene="mobC"
                     /locus_tag="MB219_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCC8428452.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(10584..10955)
                     /locus_tag="MB219_002819"
     CDS             complement(10584..10955)
                     /locus_tag="MB219_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCC8428453.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            11100..11285
                     /locus_tag="MB219_002820"
     CDS             11100..11285
                     /locus_tag="MB219_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCC8428454.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            11443..11610
                     /locus_tag="MB219_002821"
     CDS             11443..11610
                     /locus_tag="MB219_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428455.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            12228..12887
                     /gene="vanR"
                     /locus_tag="MB219_002822"
     CDS             12228..12887
                     /gene="vanR"
                     /locus_tag="MB219_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCC8428456.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            12890..14233
                     /gene="vanS"
                     /locus_tag="MB219_002823"
     CDS             12890..14233
                     /gene="vanS"
                     /locus_tag="MB219_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCC8428457.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            14404..15210
                     /gene="vanY"
                     /locus_tag="MB219_002824"
     CDS             14404..15210
                     /gene="vanY"
                     /locus_tag="MB219_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCC8428458.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            15223..16059
                     /gene="vanW"
                     /locus_tag="MB219_002825"
                     /pseudo
     CDS             15223..16059
                     /gene="vanW"
                     /locus_tag="MB219_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         4029 a         4102 c         4365 g         3577 t
ORIGIN      
        1 gaattgcccg gtatgtaaaa ccaacctgac cgagtgcagc ggcccggagc cgcaggaacc
       61 ccagccggag gaacccgaag ccccggagga agaacccaag ggcggcgcgg gcggcctgat
      121 cgtgttcctg ctggtggcgc tggcgggcgg cggtgcggcc ctttactatt tcaagttcaa
      181 aaagcccaag gctgaaacca ccggcagcga cgatttgggc gagtatgatt ttggggagga
      241 cgaggatttg gaggacgagg aacccgaaac cgagtttgac ccggaagctg attttgtgag
      301 tgaacaggag gacgaggaat gacatataaa cttgtaatcg cggaaaaacc cagcgtggcc
      361 cgcagcattg cgggcgtgat cggggccacg gagaaacacg acggctattt ggaggggaac
      421 ggctatctgg tgagctggtg catcggacat ctggtgtcct ttgccgacgc cggacgctat
      481 gacgagcgtt tcaaaaaatg gcgctatgag gatttgccca ttctcccgga aacatggcag
      541 tacatcgtcc ccgacgacaa gaaacagcag tttgacgttc tctgttcgct gatgcagcgc
      601 cccgacgtga ccggccttgt gtgcgccacc gacgccgggc gcgaggggga attgattttc
      661 cgtttcgtct accagatggc gggatgccaa aagccctttg agcgcctttg gatttcctct
      721 atggaggaca gtgctattaa ggacggcttt gcccacctga aaccgggcgg cgactatgat
      781 ccgctgtatc aatcggccct gtgccgggcc aaggccgact ggctgatcgg cattaacgcc
      841 acccggcttt tttccgtcct gtaccacaag aacctgactg tgggccgcgt ccagacgccg
      901 accttaaaaa tgctggtgga cagggacgcc aagattaccg atttcaagaa agagaaatat
      961 cacatcgtcc atatcacggc gggcggcgcg gacgcggtaa gcagccgctt ttccgacgct
     1021 gccgaggcaa acaccgtcaa ggcggcttgt gcaaaagcgc aggccgtttg cgtttccgtg
     1081 acgcgggaga aaaagacgga acagccgccc aagctctatg accttaccac cttgcagcgg
     1141 gaggctaacc ggcttttcgg ctataccgcc aagcagaccc ttgactatgc ccaggcactg
     1201 tatctaaaga agctgctgac ctatccgaga acggatagtc aatatcttac cgacgatatg
     1261 ctgcccgccg tggaaagcct tgtgtccggc ctgtgggagc tggtgccgtt tgccaagggc
     1321 ctgaaccttt ccccgcagtt tgaccgcatc ctgaacagca aaaaggtgtc cgaccaccac
     1381 gccattatcc ccaccgctga atttgtgaag cagggctttg gcactctggc cgagagtgaa
     1441 aaaaagctgc tgtccctgat ctgctgcaag ctcttatgtg ccgtggccgg gccgcatatc
     1501 tatgaggccg tcaccgccac atttacctgt gccgggcagg agtttaccgc caagggcaag
     1561 accgtccttt cccccggctg gaaagagatt gaacgccggt tccgttcttc cttaaagacc
     1621 gacgccgacg aggacgccga ggcggtgcgg gagctgccgg agctttgcga gggccagacc
     1681 tttgcagacg tggccgcctc tgtcaccgag catttcacca cgccgcccaa gccctacacc
     1741 gaagatacgc ttttgtccgc tatggaacgg gccggggccg aggatatgcc ggaggatgcc
     1801 gagcgcaagg gattaggcac accggccacc cgcgccgcca ttctggaaaa gctggtgcag
     1861 atgggctttg tccagagaaa aggcaagcag ctcattccca ccaaggacgg catcaatctg
     1921 gctgtggtgc tgccggaggc gcttacttct ccccagctta ctgccgaatg ggaaaaccgg
     1981 ctgaccgaga tcgccaaggg gcaggccgat cctgacgaat ttatggacgg catcgagacg
     2041 caggcgcggg agctggtgca gacctattcc tgtatcagtg aggaaaagca gaaattgttt
     2101 caggccgagc gtgttcctat cgggacgtgt ccccgctgtg gtgaggctgt ctatgagggc
     2161 aagaaaaact actactgcgg aaaccgggcc tgccagttcg tcatgtggaa gaatgaccga
     2221 ttttttgagg aacgcaagaa agcgttcacc ccgaagatcg ccgctgcgct gctcaaaacc
     2281 ggcaaagtca aggtaaaggg cctctattcc gtaaagaccg gcaaaaccta cgacgggacg
     2341 gtgcttttgg ctgataccgg gggcaagtac gtcaactacc gcattgaaca caggagctaa
     2401 aggacagtaa cacagcaagc atagggagtg tggctacact cccggtaaaa tcccccggcc
     2461 gcgtccctgc cgggccgggg atttttgctt gttggggaag tccccaaccc cctatttttt
     2521 gaaacagaac agagaaagga gtgaccgcgt gaacgaagta ttttctatcc aagtccataa
     2581 ccggcagttt ttggaacagg gcaaacaagg cgtctggctg gatttgccca ccacaaccga
     2641 aacgctgcaa gccgccctgc gggatgttgg cattactgcc gacaatccgc aggatttttt
     2701 tatcaacggg tattcctgcc cggaggacag acaccttgcc attccctatg acatggtgct
     2761 ggccgccgac gtggacgagc tgaatttcct tgccgcccgg ctgggccagc ttgacgccgc
     2821 cgagatcgca gagctgaacg ccgccctgca aaatccccgt ggcggctttg agaatatcgg
     2881 ccagatcatc gactacaccg aaaatgtgga ttactatgtg catcttcccg acgttagggg
     2941 ggatggacag ttgggcgact actacctgaa ccgttccggc atggtggata tgccggagga
     3001 atggaaagcc gggattgata cggcgcagtt tggacgccac attgcccagc aggaacaagg
     3061 cgtttttacc cagtacggct atctggtgag aagcggcgac gaatggcagc gcgtccatga
     3121 gggccagccg gtgccggagg aataccgggt gatgcactac ccccagccgg aagctgaccg
     3181 ggaggaaccg caggcccagc agccggaggc atccgaaagc cccgacaaag cgccgccccc
     3241 ggttatcccg atcatgctgg acggcatgaa cagcaaagaa cgcatgaaag tgattactga
     3301 ccgtctggaa accggcgtac aggagctttt tgaaagtagc cgctatatgg cttacttatc
     3361 cgctatgtcc aagatgcacg attacagtct gaacaatacg ctgctgatcg ccatgcaggg
     3421 cggttcgatt gtggcgggct ataacaggtg gcaggatgaa tttgaccgcc atgtgaagcg
     3481 gggcgagaaa ggcattaaga tactggcccc ggccccgttc aaggttaagc agcagatgga
     3541 gaaaatcgac ccggccaccc agcagcccgt gatcggcgcg gacggcaagc ccgtgaccga
     3601 agaacaggaa gtgacggttc cggcctttaa ggtggtgaca gtctttgaca tcagccagac
     3661 cgagggcaag gattttccca tgccctatat cagtgaactt accggcgacg tgcagcagta
     3721 tcaggatttc tttgccgcgc tggaacggac atcaccctat cccataagca ttgagccgat
     3781 ttcagacggg gccaagggcc gctgcttcta cgcaaaacag cgcattgcca tcaacgaggg
     3841 catgagcgaa ttgcagaccc taaagaccgc catccatgag attgcccatg ccaagctcca
     3901 cgccattgac cgggaggccc ccgccgagga acaggcggcc cggccagacc gcagcacccg
     3961 cgaggtacag gccgaaagcg tggcctatac cgtctgccag tattacgggc tggacacgtc
     4021 ggactattct tttggctatg tggccgattg gagcagcggc agggagttat ccgagctgaa
     4081 agcctccctt gaaaccatcc gggccacggc cagcgaattg ataaccgaga tcgacggcca
     4141 ttttgcccag cttcagcagg agcgcgaggc ccggcaggaa caggaggccg tcacggttga
     4201 cgttaccccg gaggcagcgg ccccggcgca ggccgaggaa gaacaggccc cggcccttga
     4261 tccggcagcc gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg
     4321 ccagcaaatg ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc
     4381 ggcagaggca ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc
     4441 ggacagctac gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat
     4501 ccagagctac caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac
     4561 aaccggcata gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt
     4621 tgtcccctat atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg
     4681 cttacagtcc ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc
     4741 ttatgtgcag gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc
     4801 gaaactggct gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat
     4861 cgcgcaggag gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga
     4921 agcaccccaa caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga
     4981 ctatcggttt gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa
     5041 ctatgacctt gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg
     5101 cacattcaac atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt
     5161 ggtggtgctg caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat
     5221 ggaagtgccg gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca
     5281 gaacgaaaac atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga
     5341 acccgaacag ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc
     5401 cccggaggct gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc
     5461 ccgccgcgcc aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga
     5521 ataccggcac tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga
     5581 gccagagtat cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga
     5641 gaacatgaat aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc
     5701 gtccaatttc ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga
     5761 ggaatttcag tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat
     5821 cagcgccgac gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc
     5881 gtcgcaggag ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg
     5941 atgcccccat ctgaccgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg
     6001 gcactatgag aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg
     6061 ccggttaaag ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg
     6121 ggagtttgac ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt
     6181 tgaggggaag ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc
     6241 gcccaaggcc ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg
     6301 tatcaagtgc atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg
     6361 cagggagaaa gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc
     6421 caagcagcca ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt
     6481 tgaaaatgtg gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta
     6541 tcaaagtgac tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc
     6601 cgcagacaag accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg
     6661 tgatgttttc ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac
     6721 ccgtgacaag atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg
     6781 gggcaatctc tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca
     6841 gccggtgagt gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca
     6901 gcgttttgac ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc
     6961 ccatgacccg gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc
     7021 cgtccccggc gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga
     7081 ggcccggcgt attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac
     7141 gcatttcatg gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga
     7201 ccggctcttt gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca
     7261 tggcgtgtat gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg
     7321 gccctctatc cgcaatcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc
     7381 ggccagaacc aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga
     7441 agaaaacctg atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac
     7501 cgccgtcatg ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa
     7561 gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata
     7621 acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt
     7681 tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct
     7741 tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg
     7801 taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt
     7861 ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt
     7921 ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg
     7981 ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt
     8041 ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc
     8101 accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct
     8161 gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga
     8221 aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag
     8281 gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt
     8341 tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg
     8401 cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac
     8461 gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac
     8521 agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa
     8581 atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa
     8641 tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt
     8701 ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca
     8761 cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat
     8821 acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg
     8881 ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg
     8941 tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca
     9001 cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc
     9061 ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca
     9121 ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct
     9181 ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg
     9241 ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc
     9301 tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca
     9361 atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg
     9421 gcttgatctc atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct
     9481 gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct
     9541 gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc
     9601 cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg
     9661 aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt
     9721 ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg
     9781 caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt
     9841 gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa
     9901 attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag
     9961 ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta
    10021 tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta
    10081 caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg
    10141 tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat
    10201 gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc
    10261 aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt
    10321 tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg
    10381 gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc
    10441 ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa
    10501 atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct
    10561 aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca
    10621 attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt
    10681 ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta
    10741 aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg
    10801 ggaatggccc cgtcgttttc gatgttggca agatagcgcc agtcaatatt tattttctct
    10861 gccattgctt tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc cgcgccgaaa
    10921 gtttcaaagc cgggacaatc ttcaatattc gccatatcgc acctcccact tacattgtat
    10981 atttcatact ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt tatatttcac
    11041 attttgtttc ttttattctt cgagcaaaag aattataatg taccctgtgg aggtgtacta
    11101 tggactacat gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca cgatggataa
    11161 actactattg tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg atctggctca
    11221 ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag gcggttactg
    11281 aatagtataa tgaaaccggc gcattttgga tatggttttg aagagagaga aagcatggta
    11341 ttttgtcacg gaaaaactat aagatgatag atggcaggct gctccagacc gataaaaagt
    11401 attctcatct gaaacagaaa gaaaagattt acacatggat gtatgaagaa gcgaaacttt
    11461 atcatgacag aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc tgtatcagcc
    11521 gtatatggta gaattgaaaa ggccggcatc tggattcttt atggtgaagt ctccagacat
    11581 tggataactg ctggtctaat gcgcctttga gtatggggtg attgatttta ataccgggga
    11641 ggtacggctt tttgaggaaa atattctggg atttacattt ggcgattatt acatttcctg
    11701 taactgcaag gacacggatg atgaagatat gaggaatata gacttgctga ttttctactg
    11761 tcttgaacgg tattaggact gaatctgctg cgcggagtgc ggcaagagcg accggaggga
    11821 gtacacacag gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg caaaaaagag
    11881 gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa cgccgcaggc
    11941 gggcaagtgg tggatcagaa tatccgtaaa ggatattctg atccattgct tgccagtatt
    12001 atcgggatgt tttgacaacc gctgtaatcg aaacatccca aacattaaga aggtgttaat
    12061 tattattatc ttgatttaat tcgtttaaac caataatata gatacataca ccatgaaaag
    12121 aagcgagtat cccaatgacg gatgattaaa atccgaaaag aaatcgcttt gaaactacag
    12181 ggaaactaca gactgttatg ttatcttctt aaaatggagg gatttttatg tcgatacgaa
    12241 ttctacttgt cgaggatgat gatcatatct gtaatacagt aagggcgttt ctggctgagg
    12301 caggatatca ggtggatgcc tgcacagatg gaaatgaggc atacaccaag ttttacgaaa
    12361 acacttatca actggttatt cttgatatta tgctgcccgg tatgaacggg catgaacttt
    12421 tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat gatgacagcc ctgtcggatg
    12481 acgaaaacca aatccgggcg tttgatctag aggcagacga ctatgtaaca aagccattca
    12541 agatgcagat tttactaaag cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa
    12601 aggaaatccg tgtcggcagg ctgacacttc tgccggagga tttcacggta ctttgtgacg
    12661 gtacagagct gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca
    12721 aaggcagaac cttaacgcat gaaatcattt tgtcacgtat atggggatat gactttgaga
    12781 gtgatggcag cacagtccac actcatatca aaaatttgcg ggccaagctg ccggaaaata
    12841 tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa tggaaagaaa
    12901 agggattttc attaaggttt tttcctatac gatcattgtc ctgctactgc ttgtcggtgt
    12961 aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg aattacagca
    13021 aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata ggcttgatat
    13081 tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt atattgaaga
    13141 taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata gttttagacc
    13201 cgactttctt tatgtggtac atagagatga taatatttcg attgttgctc aaagcaaggc
    13261 aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga ttgcgataat
    13321 ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa
    13381 agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga
    13441 gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg tcaggctgaa
    13501 agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg aggaaacaca
    13561 gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt
    13621 tctgttggag ggaatgcttg aaaatatcgg tgactacaaa gatcattcta agtatctgcg
    13681 cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac tggagcttgt
    13741 cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag ggcgcacggt
    13801 tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac
    13861 agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc
    13921 caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac ggatatggag
    13981 tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc acattgatga
    14041 tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa
    14101 aagtgggcga aacggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
    14161 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
    14221 atcaacattg taaatattta aaacttaaat gattttgacc gacaggtata accctgccgg
    14281 tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt
    14341 gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat
    14401 caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa
    14461 tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg
    14521 tttttggggc ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca
    14581 actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc
    14641 gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc
    14701 atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt
    14761 cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg
    14821 attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt
    14881 gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg
    14941 gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac
    15001 ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt
    15061 ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg
    15121 tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc
    15181 cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatggacag aaaaagattg
    15241 acacagcgtt tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat
    15301 gcgggaatga gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc
    15361 catttgctct ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata
    15421 taccaggaaa acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg
    15481 ataagaccgg gggaaacctt ttctttctgg tggctggtac gccatgcgga caaagatacc
    15541 ccctataaag acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt
    15601 atgtgccaga tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc
    15661 cagcgcagag gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg
    15721 gtggatgcaa ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc
    15781 acctaccaaa tatgagtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac
    15841 aaagagcccc aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt
    15901 ggcgggattt atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa
    15961 ataatagatt gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa
    16021 agtgtggata ttcagtctaa tggtgataag tcaagatgaa atttaagatt ttc
//
LOCUS       DAHEYX010000418         4276 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.197, whole genome shotgun sequence.
ACCESSION   DAHEYX010000418 DAHEYX010000000
VERSION     DAHEYX010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.197"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            259..723
                     /locus_tag="MB219_002826"
     CDS             259..723
                     /locus_tag="MB219_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCC8428459.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            707..1336
                     /locus_tag="MB219_002827"
     CDS             707..1336
                     /locus_tag="MB219_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCC8428460.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1690..3168
                     /gene="msr(C)"
                     /locus_tag="MB219_002828"
     CDS             1690..3168
                     /gene="msr(C)"
                     /locus_tag="MB219_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCC8428461.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3215..3661
                     /locus_tag="MB219_002829"
     CDS             3215..3661
                     /locus_tag="MB219_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCC8428462.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3860..>4276)
                     /locus_tag="MB219_002830"
     CDS             complement(3860..>4276)
                     /locus_tag="MB219_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCC8428463.1"
                     /translation="HSQIYTTLAKLDQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEW
                     IKEETPAPTQKDEFLAKIYAFSTLDKTTATGLLFTREQQLNKIMQKNQKKLNDLSLDK
                     KQEFGRYVVIQRRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT         1383 a          714 c          883 g         1296 t
ORIGIN      
        1 gaaatttttg gcggtgtaaa attttctact gcagaagagc tatcttctat agtttgataa
       61 gaatcaataa tcttggtttt agcgttgtgc tttctttatc attttttccg ttcattctac
      121 aaaaaatttc ttttctctat acttttctat tgagagcgtt ttttttatta cacagctgat
      181 tctttgactt ttttcttttt ttattctatt attgacaagt caatcgttga cgagtcaatt
      241 attagaaagg aggaaattat gtctttagaa gagattagtc accttttata tcaaatcaaa
      301 ttagccgatc aaaaaatcgt ccagatcttt gaagaaaata tcggtataag tcttactaga
      361 tacgagatca tgatgagact gaaagaagaa aatccattgc tacaaagcag tttgcaagaa
      421 aagttgcaga ttgatcaagg agcagttact cgtcatctga aggtattgga agcaaatggc
      481 tatgtttcgc gtcagagaaa tcctcagaac aaccgggaag tgtatgtagc gctaacagaa
      541 aaagcccaga agaaaatcga gcattgtgaa atggaccata aagatacagg ttccgtgcta
      601 tccccggctt tcagcgatgt agaaatgaaa caatttctaa cacttttaga taaattgaat
      661 acccaacttt gcaatataac aacagaaacg aaagaggtta ataataatgg aacaaaaaaa
      721 tgatttttca acaattatga ctggtagaag atccgttcgc gtatatgatg aaacatacaa
      781 aatcccacac gaagaaatgc tggaaatgat caaagaagcg acgactgcac cttcttctgt
      841 caatatgcaa ccatggcgct ttgtggtcgc tgaaagcgag gaagcaaaag aaatcttgcg
      901 tcctttgatc cgtttcaata cgagacaaaa tgatacttct tctgcaatgg tcatgatttt
      961 tggcgatatg caatgttacg aatatggcga agaaatctat aaccaagctt acgaatcagg
     1021 gaagatgcca aaagaagtca gagatcaaca attagctgcg atcattcctt attacaagag
     1081 tttttcaaga gaagaaatga acgatgtagt caaagtcgat tctagcttag cggctatgca
     1141 attcatgtta gttgcgcgtg atcatggcta tgaaacaaat ccaattggtg gattcgaagc
     1201 agatcagttg gcagaggcat ttggtttgga taaagaccgt tatgttccag ttattatttt
     1261 atcaatagga aaagcggtag aagaaggtta cgagtctgtt cgtttagcac cagaaaagat
     1321 cactacgttc gaataaaagg ttttcagtta ctattcagta gctattgctt tttttgaatc
     1381 gtgtgtggta acctaataaa aaaggaggtc ggaaaaatga ctgcatcgat gaaattacgt
     1441 ttcgaacttt tgaataacaa ctaaataaat tcaaaagaca tgtataaaca acaatttcat
     1501 ggatggagta tgtccatttt ttcgaacgac agtagaatag gtgaaagcta tgacagattt
     1561 ggtgatttag tcatagttat ttcgttcaga gtaggaaaag atgtgatgca gtttttttgc
     1621 aaacagattt ttctgctctt ttttagtatc gataattgtt gttttacatg aaataggagt
     1681 gtaaaacata tggaaaattt agcagtaaat ataacaaatc tacaagtcag ctttggcaac
     1741 cagctagaac tatctattga ttctcttcgt gtctatcagc aagatcgtat agggatcata
     1801 ggagaaaatg gtgtcggtaa atcgactttg ctcaaactaa tagccggtga actttttccc
     1861 gatcatggga aaatccaaac agagatcacc ttcaactacc tgcctcaatt aacctatctc
     1921 gctgaagcaa aggacctaaa tttggaatta gccagtcatt tccagttaag actggaagaa
     1981 acttcagagc ggaaatggag cggaggggaa gaacgaaaga tcgagttgat acgtcttctt
     2041 tcttcttatg aacaagggat gcttctagac gagccaacaa cccatctaga tagaaaaagt
     2101 attgatcgac tgattgaaga gcttcgttat tattatggta cgctggtttt tgttagtcat
     2161 gatcgctatt ttctagatga gctagcatcg aaaatctggg aagtaaaaga cggagaaatc
     2221 cgagagtttt cggggaatta tagtgcctat ctcactcaaa aggaattgga gaaaaagact
     2281 cagctacgag aagcagagtc gatcatgaaa gagaaaaaac gattggaaaa atcgatccaa
     2341 gaaaagaaaa aacaagcgga aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa
     2401 atcagaccgg atcggttgtc ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa
     2461 aagaatgcga aaacattaga gagaagactc caaaaaatag gagaaacaac caagccgcag
     2521 cagatgaaac aaatccgttt tccagtacca aaatctcttg aactccacag ccgctatccc
     2581 atcatgggac aaaatgtcca attggaaaga agtgggagaa cattactggt aaatggtgat
     2641 tttcagtttt ctttaggtaa aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca
     2701 accttattag aacatatccg caaacaagga gaaggaatcc ttctctctcc gaaagtaagc
     2761 tttcaagtat atcagcaaaa gggttatcaa atgacatctg aagaatccat cattcgtttt
     2821 gtcatgagac aaacagagtt ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg
     2881 tttgctcagg aaactttgac gaaaccgtta tgtacattaa gtgggggaga agcgacccgt
     2941 ctgacgattg ctttgctttt tactaagcca agtaatgtgt tgctgttaga tgaaccgact
     3001 aattttattg atatggcaac gatcgaagct ttagagaagc taatgcaaat atatccggga
     3061 acgattttgt ttacttctca tgattcttac tttgtcgagc gtacggctga tgaagtttat
     3121 gaaataaaag ggcagaaaat aaaaaaagta cttacgagaa atttttaaat gtgctacgat
     3181 gattttcgtg acaaatttaa aggagtggta cagattgcct acaaataaaa aagaaggaat
     3241 aatttttaca acaatgatgt gttttttgat ggtcttgggg atgagcgcgt ataatttatg
     3301 gctgcatcaa gatcttcatt tatcaagttt gttgattggt ctagtgcctg gattcgtggt
     3361 ggctttcatt ttggatgtct tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact
     3421 accagtaaat ccagaaagca aggtccaaat gatcttaact atttcatgct tgatggtttt
     3481 aggaatggtg acatgtatgt ctttattcgg tgtgatcatg gaacaaggaa tacctgatca
     3541 attattctct gcttatatga atgcttggaa aatgaatgtg attgccgctt taccgttgca
     3601 gttgatcatc gttggcccgc tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg
     3661 atggaacact gttatttgaa aaaaggcctt ggcatgcttt ttgtcatagt ctcttgaatg
     3721 cagttctaga aaaatagtga tccgaacgat aggttttggc ctgtcgttcg gatcgctttt
     3781 tcttttccaa ctgtattgct cttttattga agataatttg ctcttcaata gatatttcta
     3841 gaaaggttct gtttatccct tactcaaaaa actgctgcat ttgttctctt acttgctgac
     3901 accattggag ttcctgctga caaagcataa ttctgcgctg gatcacaaca taacgtccaa
     3961 attcttgttt cttgtctagc gagaggtcat tcaatttttt ttgatttttt tgcatgattt
     4021 tgttcagctg ttgttctcta gtgaatagca gtccagtagc tgttgtttta tcgagcgtcg
     4081 aaaaagcata gatttttgct agaaattcat ctttttgcgt aggagcggga gtttcctctt
     4141 tgatccattc ctctacaaga gcacgtcctt catcagtcaa gtggtagatc tttttttgag
     4201 agtgctcgtc tttgtccaaa acggtcacat aaccctgctg gtctaacttg gcaagcgttg
     4261 tatagatctg actgtg
//
LOCUS       DAHEYX010000419         3029 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.222, whole genome shotgun sequence.
ACCESSION   DAHEYX010000419 DAHEYX010000000
VERSION     DAHEYX010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.222"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            <1..701
                     /locus_tag="MB219_002831"
     CDS             <1..701
                     /locus_tag="MB219_002831"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCC8428464.1"
                     /translation="GGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLII
                     AADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSG
                     AAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYG
                     VAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKI
                     LGQTLSRFFTILPK"
     gene            959..2461
                     /gene="eat(A)"
                     /locus_tag="MB219_002832"
     CDS             959..2461
                     /gene="eat(A)"
                     /locus_tag="MB219_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCC8428465.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2528..>3029
                     /locus_tag="MB219_002833"
     CDS             2528..>3029
                     /locus_tag="MB219_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCC8428466.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVD"
BASE COUNT         1036 a          532 c          679 g          782 t
ORIGIN      
        1 atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa
       61 atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag
      121 taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt
      181 ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag
      241 caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag
      301 gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt
      361 ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg
      421 tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg
      481 gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag
      541 accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc
      601 caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag
      661 gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt
      721 tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag
      781 tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga
      841 ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg
      901 catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat
      961 gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt
     1021 tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg
     1081 tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt
     1141 aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac
     1201 ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca
     1261 attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga
     1321 gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga
     1381 tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa
     1441 gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga
     1501 ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta
     1561 tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa
     1621 agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga
     1681 aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa
     1741 aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg
     1801 gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc
     1861 tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca
     1921 attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg
     1981 ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg
     2041 ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta
     2101 tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca
     2161 agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt
     2221 tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc
     2281 tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt
     2341 caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga
     2401 acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta
     2461 acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga
     2521 gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt
     2581 ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc
     2641 agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg
     2701 agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga
     2761 acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt
     2821 tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt
     2881 gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct
     2941 gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa
     3001 tgaggggttc tttacattca tggtagata
//
LOCUS       DAHEYX010000420         3027 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.225, whole genome shotgun sequence.
ACCESSION   DAHEYX010000420 DAHEYX010000000
VERSION     DAHEYX010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.225"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            47..1018
                     /locus_tag="MB219_002834"
     CDS             47..1018
                     /locus_tag="MB219_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCC8428467.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="MB219_002835"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="MB219_002835"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCC8428468.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="MB219_002836"
     CDS             2045..2653
                     /locus_tag="MB219_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCC8428469.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2719..2892)
                     /locus_tag="MB219_002837"
     CDS             complement(2719..2892)
                     /locus_tag="MB219_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCC8428470.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT          814 a          635 c          828 g          750 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgtt
//
LOCUS       DAHEYX010000421         2098 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.264, whole genome shotgun sequence.
ACCESSION   DAHEYX010000421 DAHEYX010000000
VERSION     DAHEYX010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.264"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            203..1243
                     /locus_tag="MB219_002838"
     CDS             203..1243
                     /locus_tag="MB219_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCC8428471.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1341..1529
                     /locus_tag="MB219_002839"
     CDS             1341..1529
                     /locus_tag="MB219_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCC8428472.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1587..1940)
                     /locus_tag="MB219_002840"
     CDS             complement(1587..1940)
                     /locus_tag="MB219_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCC8428473.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          691 a          331 c          374 g          702 t
ORIGIN      
        1 tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa aatgaaaaaa
       61 gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact acttacttgc
      121 atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag tatcagaaaa
      181 agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat gtaagattga
      241 tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt taaatagtgt
      301 tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc accctctact
      361 acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg atgccctttt
      421 ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga cacatgaaat
      481 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
      541 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
      601 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
      661 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
      721 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
      781 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt acggtttata
      841 ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg tactggagca
      901 agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta aagtttatgc
      961 accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg caaatatcgt
     1021 aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg ctcgatgtat
     1081 tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt gtttagcaga
     1141 gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc gtctaaatag
     1201 tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt tatgttgtta
     1261 tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat ttgaattaaa
     1321 gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct atttgtggaa
     1381 ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct ttatactgcc
     1441 ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata acaatcaaag
     1501 agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat cttatcggct
     1561 ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc attgatacct
     1621 tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat gtatttttct
     1681 acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa ttcatcaata
     1741 gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg ttgtcctttg
     1801 ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac ttgttcacga
     1861 gtgagacccc gtttcattcg agcctcttta atcgctaaac caacggctct aaaatcgtat
     1921 ttatcttctt ttttacgcat actaaatcac ctctatacat tttactgttc ctatataacc
     1981 ataaacaggt atagaaaaac atgttatata gtttatgagt tcctatagaa cactgtggaa
     2041 tttattaatg cgatgaatcc aatttcaaat agaaaggtcg ctgattttat ttacctat
//
LOCUS       DAHEYX010000422         2028 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.266, whole genome shotgun sequence.
ACCESSION   DAHEYX010000422 DAHEYX010000000
VERSION     DAHEYX010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.266"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            97..1470
                     /gene="tet(L)"
                     /locus_tag="MB219_002841"
     CDS             97..1470
                     /gene="tet(L)"
                     /locus_tag="MB219_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HCC8428474.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          588 a          302 c          413 g          725 t
ORIGIN      
        1 gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat attttttaat
       61 aaagatttga attaaagtgt aaaggaggaa ttaattatga agtgtaatga atgtaacagg
      121 gttaaataca accaaatttt aatttggctt tgcgttttat ctttttttag cgtattaaat
      181 gaaatggttt tgaacgtctc attacctgat attgcaaatg attttaataa accacctgcg
      241 agtacaaact gggtgaacac agcctttatg ttaacctttt ccattggaac agctgtatat
      301 ggaaagctat ctgatcaatt aggcatcaaa aggttactcc tatttggaat tataataaat
      361 tgtttcgggt cggtaattgg gtttgttggc cattctttct tttccttact tattatggct
      421 cgttttattc aaggggctgg tgcagctgca tttccagcac tcgtaatggt tgtagttgcg
      481 cgctatattc caaaggaaaa taggggtaaa gcatttggtc ttattggatc gatagtagcc
      541 atgggagaag gagtcggtcc agcgattggt ggaatgatag cccattatat tcattggtcc
      601 tatcttctac tcattcctat gataacaatt atcactgttc cgtttcttat gaaattatta
      661 aagaaagaag taaggataaa aggtcatttt gatatcaaag gaattatact aatgtctgta
      721 ggcattgtat tttttatgtt gtttacaaca tcatatagca tttcttttct tatcgttagc
      781 gtgctgtcat tcctgatatt tgtaaaacat atcaggaaag taacagatcc ttttgttgat
      841 cccggattag ggaaaaatat accttttatg attggagttc tttgtggggg aattatattt
      901 ggaacagtag cagggtttgt ctctatggtt ccttatatga tgaaagatgt tcaccagcta
      961 agtactgccg aaatcggaag tgtaattatt ttccctggaa caatgagtgt cattattttc
     1021 ggctacattg gtgggatact tgttgataga agaggtcctt tatacgtgtt aaacatcgga
     1081 gttacatttc tttctgttag ctttttaact gcttcctttc ttttagaaac aacatcatgg
     1141 ttcatgacaa ttataatcgt atttgtttta ggtgggcttt cgttcaccaa aacagttata
     1201 tcaacaattg tttcaagtag cttgaaacag caggaagctg gtgctggaat gagtttgctt
     1261 aactttacca gctttttatc agagggaaca ggtattgcaa ttgtaggtgg tttattatcc
     1321 atacccttac ttgatcaaag gttgttacct atggaagttg atcagtcaac ttatctgtat
     1381 agtaatttgt tattactttt ttcaggaatc attgtcatta gttggctggt taccttgaat
     1441 gtatataaac attctcaaag ggatttctaa atcgttaagg gatcaacttt gggagagagt
     1501 tcaaaattga tccttttttt ataacaggaa ttcaaatctt tttgttccat taaagggcgc
     1561 gattgctgaa taaaagatac gagagacctc tcttgtatct tttttatttt gagtggtttt
     1621 gtccgttaca ctagaaaacc gaaagacaat aaaaatttta ttcttgctga gtctggcttt
     1681 cggtaagcta gacaaaacgg acaaaataaa aattggcaag ggtttaaagg tggagatttt
     1741 ttgagtgatc ttctcaaaaa atactacctg tcccttgctg atttttaaac gagcacgaga
     1801 gcaaaacccc cctttgctga ggtggcagag ggcaggtttt tttgtttctt ttttctcgta
     1861 aaaaaaagaa aggtcttaaa ggttttatgg ttttggtcgg cactgccgac agcctcgcag
     1921 agcacacact ttatgaatat aaagtatagt gtgttatact ttacttggaa gtggttgccg
     1981 gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgatt
//
LOCUS       DAHEYX010000423         1941 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.271, whole genome shotgun sequence.
ACCESSION   DAHEYX010000423 DAHEYX010000000
VERSION     DAHEYX010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.271"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB219_002842"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB219_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCC8428475.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          666 a          311 c          405 g          559 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta g
//
LOCUS       DAHEYX010000424         1941 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.272, whole genome shotgun sequence.
ACCESSION   DAHEYX010000424 DAHEYX010000000
VERSION     DAHEYX010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.272"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="MB219_002843"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="MB219_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCC8428476.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          665 a          311 c          406 g          559 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta g
//
LOCUS       DAHEYX010000425          975 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.351, whole genome shotgun sequence.
ACCESSION   DAHEYX010000425 DAHEYX010000000
VERSION     DAHEYX010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.351"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            45..956
                     /gene="aph(2'')"
                     /locus_tag="MB219_002844"
     CDS             45..956
                     /gene="aph(2'')"
                     /locus_tag="MB219_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ie/IVa family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCC8428477.1"
                     /translation="MTTYTFDQVEEAIEQLYPDFTINTIEISGEGNDCIAYEINGNFI
                     FKFPKHSRASINLLNEVTVLKTIHNELSLPIPEVVFTGMPSEMCQMSFAGFTKIKGVP
                     LTPLLLKNLPKQSQDQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKL
                     LSRELKGHQMKKVDDFYRDILDNEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDF
                     GDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYMMKEKYWSFEKI
                     IYGKEYGYMDWYEEGLNEIRSSELLTT"
BASE COUNT          360 a          152 c          182 g          281 t
ORIGIN      
        1 atttacagtc aatcagattt ttgattaaag ggagtagaga agacatgaca acttatactt
       61 tcgaccaggt agaagaggca atagagcagt tatatcctga ttttactatc aatacaatag
      121 agatttcagg agaaggcaat gactgtattg catatgaaat aaacgggaat tttattttta
      181 aatttccaaa gcattcaaga gcttcgatta atctcttgaa tgaagtaacc gtactcaaaa
      241 caatccacaa tgaattatca ctacccattc ccgaggtggt ttttacagga atgccatcag
      301 aaatgtgcca aatgtctttc gcaggtttta caaaaattaa aggagtacct ttgacacctc
      361 ttctactcaa aaatctgccg aagcaatctc aagatcaggc agctaaggac ctggcccgat
      421 ttttaagtga acttcacagc ataaatatct ctggattcaa aagtaatctg gtattagatt
      481 ttcgagagaa gataaatgaa gataataaaa aaatcaaaaa gttactatcc agggaattaa
      541 agggtcacca gatgaagaaa gtggatgatt tttacaggga tattctagac aacgaaatct
      601 acttcaaata ctatccttgt cttattcata acgatttcag cagcgatcat attttatttg
      661 ataccgaaaa aaataccatt tgtggaataa tcgattttgg agatgcagct atttctgatc
      721 ccgacaatga ttttataagt ttgatggaag atgatgaaga gtacggcatg gaattcgtat
      781 caaaaatatt gaaccattac aaacataagg atataccgac agttttggaa aaatatatga
      841 tgaaagaaaa atactggtcg ttcgaaaaga ttatctatgg aaaggaatat ggttatatgg
      901 attggtatga agagggatta aatgaaatca gaagcagtga actactcacc acttagctaa
      961 acctgacggt tctta
//
LOCUS       DAHEYX010000426          899 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.359, whole genome shotgun sequence.
ACCESSION   DAHEYX010000426 DAHEYX010000000
VERSION     DAHEYX010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.359"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            286..834
                     /gene="aac(6')"
                     /locus_tag="MB219_002845"
     CDS             286..834
                     /gene="aac(6')"
                     /locus_tag="MB219_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCC8428478.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          321 a          129 c          186 g          263 t
ORIGIN      
        1 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
       61 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
      121 ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag taagataaat
      181 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
      241 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
      301 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
      361 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
      421 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
      481 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
      541 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
      601 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
      661 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
      721 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
      781 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
      841 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAHEYX010000427          739 bp    DNA     linear   BCT 17-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium strain M00680
            SAMN25126087-rid13882913.guided.376, whole genome shotgun sequence.
ACCESSION   DAHEYX010000427 DAHEYX010000000
VERSION     DAHEYX010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25126087
            Sequence Read Archive: SRR17671386
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242173.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242173.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/08/2022 00:47:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,846
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,733
            CDSs (with protein)               :: 2,733
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 26 of 55
            Pseudo Genes (incomplete)         :: 20 of 55
            Pseudo Genes (internal stop)      :: 18 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25126087-rid13882913.guided.376"
                     /strain="M00680"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Brisbane"
                     /collection_date="2020-01"
                     /collected_by="Pathology Queensland"
     gene            194..691
                     /gene="dfrG"
                     /locus_tag="MB219_002846"
     CDS             194..691
                     /gene="dfrG"
                     /locus_tag="MB219_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCC8428479.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          291 a           71 c          152 g          225 t
ORIGIN      
        1 attacataca aaatgattta taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg
       61 attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa ctaacgggca
      121 ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa
      181 ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg
      241 caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac
      301 aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc
      361 tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat
      421 tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt
      481 cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa
      541 aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa
      601 tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca
      661 tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa
      721 agataaagca caagagctt
//
